{"data":[{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":163,"idioma_id":5,"titulo":"Estudio clínico de felinos infectados naturalmente con Aelurostrongylus abstrusus.","resumen":"Aelurostrongylus abstrusus is a nematode, with an indirect life cycle, that infects the respiratory tract of wild and domestic cats. It may cause bronchopneumonias of several degrees. Cats are infected through ingestion of slugs and\/or snails with the infective larvae of A. abstrusus. The aim of this study was describes five cases of aelurostrongylosis in stray cats in Buenos Aires City. Clinical symptoms were evaluated and thoracic radiographs were obtained from all cats.  Stool and blood samples were taken for parasitological diagnosis and haematological studies. Samples of the lung tissue were obtained for recover larvae and perform histopathologic postmortem studies. The symptoms observed were: cough, dyspnea, sneezing and bodyweight loss. Two cats showed severe disease and respiratory failure. Radiographic findings included bronquial thickening and peribronchial infiltrate. Larvae of A. abstrusus were detected in fecal samples of three cats. All cats were eosinophilic. The necropsy showed numerous yellow-white granulomas distributed throughout the surface of the lungs and the histopathology study showed extensive parenchymal areas and intralesional cross-sections of larvae with abundant cellular infiltrate. This research highlights the importance of carry out a differential diagnosis of aelurostrongylosis with others diseases that affects cats.","palabras_clave":"Aelurostrongylus abstrusus|diagnóstico|Felinos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":273,"idioma_id":5,"titulo":"Revistas, consumos, alimentación y saberes femeninos. La propuesta de Damas y damitas, Argentina 1939-1944","resumen":"      El presente artículo aborda un aspecto  puntual del abanico que se extiende en torno a la problemática de la  transmisión de los saberes femeninos: la transmisión escrita del saber  culinario y sus connotaciones teóricas, prácticas, performativas y económicas.  Específicamente, nos concentraremos en la Argentina en el cruce de las décadas  del ?30 y ?40, recorte que coincide con la consolidación de la sociedad de  consumo y con la irrupción en el mercado editorial de una revista femenina  llamada Damas y damitas, que tomaremos como referente empírico de  nuestro análisis. En dicha publicación recuperamos el accionar de la ecónoma  encargada de enseñar a cocinar pero también de prescribir un deber ser y un  saber elegir (la marca) a sus congéneres.   ","palabras_clave":"MUJERES|SOCIEDAD DE CONSUMO|TRANSMISIÓN|ECONOMÍA DOMÉSTICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":352,"idioma_id":5,"titulo":"Lecturas literarias: desde las huellas en el cuerpo a los significados ideológicos","resumen":"Cuando hablamos de la lectura en términos de decodificación, la entendemos  como actividad de búsqueda de información, pero no de trabajo con los sentidos ?del texto-  ni como experiencia de resignificación de la subjetividad. Todo eso que falta, tiene que ver con una  lectura  encarnada en el texto, en la palabra y también en el sujeto, a través de la interpretación, tanto de aquellos elementos sistémicos como los no sistémicos.  Desde ?el convencimiento, según Larrosa (2007:86), de que las palabras producen sentido, crean realidad y, a veces, funcionan como potentes mecanismos de subjetivación? nuestra hipótesis es que la lectura de un texto literario deja huellas o rastros no representacionales en el cuerpo de los lectores.Nos proponemos dar cuenta de los efectos de una lectura ?sobre el texto? y también sobre el sujeto mismo, a través de las informaciones recabadas luego de la implementación de un instrumento aplicado en el último año del nivel medio, en una escuela privada de la ciudad de Catamarca.","palabras_clave":"CUERPO|INTERPRETACIÓN|LECTURA|SENTIDOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":360,"idioma_id":5,"titulo":"Estudio del efecto de bisfosfonatos y aceite de oliva en un modelo experimental de hueso en ratas.","resumen":"Introducción: El éxito de cualquier implante dental depende de una serie de parámetros incluyendo las condiciones generales de salud, biocompatibilidad de los materiales del implante, la naturaleza de la superficie del implante la microscópica y macroscópica, el procedimiento quirúrgico y la calidad y cantidad del hueso maxilar. Objetivo general: evaluar la fosfatasa alcalina en sangre para poder analizar la respuesta terapéutica del tratamiento con bisfosfonatos administrados por vía subcutánea y de Aceite de Oliva extra virgen suministrado con la dieta en animales de experimentación. Materiales y Método: Las fórmulas farmacéuticas fueron preparadas con una dosificación para Alendronato (AL) de 0,5 mg\/Kg de peso, y para Pamidronato (PA) de 0,6 mg\/Kg, Aceite de Oliva (O) se administró junto con la dieta, 50 g\/ Kg de comida. El control (C) fue solución fisiológica. El efecto se evaluó en ratas machos Wistar normales, las cuales se dividieron en cuatro grupos, uno control y tres problemas, y tres grupos más para la combinación droga (O). Se midió fosfatasa alcalina en sangre para analizar los cambios a nivel óseo. La comparación de los datos se realizó por análisis de la variancia a dos criterios de clasificación (tratamientos y tiempos de tratamiento: 0, 7, 15, 30, 60 y 90 días). Se consideraron diferencias significativas si p<0,05. Resultados: Todas las drogas presentaron niveles de fosfatasa alcalina significativamente mayores que el (C). También fueron significativas las diferencias entre PA y las otras drogas. Por otra parte no se encontraron diferencias significativas entre AL+O y O. Las diferencias de niveles de fosfatasa en relación al tiempo, es evidente que a los 7 días, los valores son significativamente menores. Las medias sufren un incremento con picos a los 30 y 45 días. A los 90 días, los niveles decaen en la mayoría de las drogas.Conclusiones: el modelo experimental con ratas machos sana y tratamiento semanal con AL y PA y (O) produjo variaciones significativas en las concentraciones minerales en sangre, revelando una acción inhibitoria en la actividad osteoclástica en todos los casos.","palabras_clave":"Marcadores óseos|bisfosfonatos|fosfatasa alcalina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":460,"idioma_id":4,"titulo":"The use of a nerve stimulation test to confirm sacrococcygeal epidural needle placement in cats","resumen":"Objective To determine if a nerve stimulation test (NST) could act as a monitoring technique to confirm sacrococcygeal epidural needle placement in cats.Study design Prospective experimental trial in a clinical setting.Animals Twenty-four adult cats, scheduled for a therapeutic procedure where epidural anesthesia was indicated.Methods Under general anesthesia, an insulated needle was inserted through the S3-Cd1 interverte- bral space guided by the application of a fixed electrical current (0.7 mA) until a motor response was obtained. The NST was considered positive when the epidural nerve stimulation produced a motor response of the muscles of the tail, whereas it was considered negative when no motor response was evoked. In the NST positive cases, 0.3 mL kg1 of 0.5% bupivacaine was administrated before nee- dle withdrawal. Ten minutes after injection, epidural blockade was confirmed by the loss of perineal (anal), and pelvic limbs reflexes (patellar and with- drawal).Results The use of a fixed electrical stimulation current of 0.7 mA resulted in correct prediction of sacrococcygeal epidural injection, corroborated by post bupivacaine loss of perineal and pelvic limb reflexes, in 95.8% of the cases.Conclusion and clinical relevance This study dem- onstrates the feasibility of using, in a clinical setting, an electrical stimulation test as an objective and in real-time method to confirm sacrococcygeal epidural needle placement in cats.","palabras_clave":"cat|epidural|nerve stimulation| sacro- coccygeal."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":491,"idioma_id":5,"titulo":"Analisis multicriterio: aplicacion al estudio de la sustentabilidad en sistemas de produccion agropecuarios en la Region Pampeana.","resumen":"El interés en el análisis de la sustentabilidad de los sistemas de producción agropecuarios se ha fortalecido a partir de evidencias de que la rentabilidad de corto plazo trae como consecuencia el incremento de la vulnerabilidad de los sistemas de producción (Manchado, 2010). En tal sentido, se han desarrollado numerosos estudios buscando compatibilizar la protección de los SE de los agro-ecosistemas con los altos niveles de producción (ya sea alimentos, fibras o biocombustibles) (Rossing et al, 2007).   En este trabajo se propone considerar y analizar los conflictos entre múltiples criterios de decisión (económicos y ambientales) para dos sistemas de producción de distintas zonas de la provincia de Buenos Aires. El enfoque con que se desarrollaron los estudios de caso fue adoptado con la intención de contribuir a generar una visión más amplia de los problemas a resolver en un sistema de producción que la meramente económica   Se aspira a generar información que sea de utilidad para formular e instrumentar incentivos o regulaciones a una mayor escala, que promuevan el equilibrio entre los factores económicos, sociales y ambientales implícitos en el concepto de desarrollo sustentable (Cisneros et al, 2010)  En ambos trabajos, La posibilidad de considerar simultáneamente más de un atributo - entre ellos, el resultado económico con un ?logro aceptable? - ha arrojado como denominador común una combinación relativamente más equilibrada entre agricultura y ganadería.    En base al conjunto de resultados de estos trabajos, es interesante plantear que los actores económicos podrían modificar sus criterios de asignación de la tierra, que den como resultados de producción más sustentables en un sentido amplio, sin provocar cambios sustanciales en la estructura productiva de sus empresas.   Para aportar una mirada hacia el futuro de los sistemas agropecuarios, considerando la posibilidad de adoptar distintos sistemas de producción que impliquen profundos cambios en el uso de los recursos naturales, probablemente sea necesario incorporar nuevas actividades y tecnologías innovadoras como opciones para el uso de la tierra (e.g., producción orgánica, cultivos de cobertura, riego complementario).    En tal caso, habría que afrontar este desafío mediante un abordaje interdisciplinario que permita - en primer lugar - arribar a coeficientes para cada uno de los atributos económicos y ambientales de estos usos alternativos del suelo, y en segundo lugar encontrar una combinación entre ellos que posibilite una mejor contribución al interés común.  Adicionalmente, existen otros atributos económicos (como el nivel de riesgo) y ambientales (como la emisión de gases con efecto invernadero) que sería importante incluir en futuros estudios con modelización multicriterio.","palabras_clave":"SUSTENTABILIDAD|ANALISIS MULTICRITERIO|SISTEMAS DE PRODUCCIÓN|REGION PAMPEANA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":540,"idioma_id":4,"titulo":"Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells","resumen":"Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide.E2 is an immunodominant protein and a promising candidate to develop subunit vaccines.To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody namedAPCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed inthe baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2vaccine was the best one to evoke a humoral response, and for this reason, it was selected fora cattle vaccination experiment. All the bovines immunized with 1.5g of APCH-tE2 developedhigh levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstratingits significant potential as a subunit vaccine. This novel vaccine is undergoing scale-upand was transferred to the private sector. Nowadays, it is being evaluated for registration asthe first Argentinean subunit vaccine for cattle.","palabras_clave":"E2 glycoprotein|Single chain antibody|Subunit vaccine|Bovine viral diarrhea virus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":565,"idioma_id":4,"titulo":"Spray drying formulation of albendazole microspheres by experimental design. In vitro-in vivo studies","resumen":"Both an experimental design and optimization techniques were carried out for the development of chitosan?pectin?carboxymethylcellulose microspheres to improve the oral absorption of albendazole as a model drug. The effect of three different factors (chitosan, pectin and carboxy methyl cellulose concentrations) was studied on five responses: yield, morphology, dissolution rate at 30 and 60 min, and encapsulation efficiency of the microspheres. During the screening phase, the factors were evaluated in order to identify those which exert a significant effect. Simultaneous multiple response optimizations were then used to find out experimental conditions where the system shows the most adequate results. The optimal conditions were found to be: chitosan concentration, 1.00% w\/v, pectin concentration 0.10% w\/v and carboxymethylcellulose concentration 0.20% w\/v. The bioavailability of the loaded drug in the optimized microspheres was evaluated in Wistar rats which showed an area under curve (AUC) almost 10 times higher than the pure drug.","palabras_clave":"ALBENDAZOLE|BIOMATERIALS|MICROSPHERES|ORAL DRUG RELEASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":578,"idioma_id":5,"titulo":"Evaluación Genética de Reproductores Holando Argentino en los ámbitos nacional e internacional","resumen":"La presente publicación se enmarcó dentro de las actividades establecidas en el Convenio Marco y Carta Acuerdo firmados entre la Asociación Criadores de Holando Argentino y la Facultad de Ciencias Veterinarias de la Universidad Nacional del Centro de la Provincia de Buenos Aires. Conforme a lo estipulado en el Decreto N 688\/81 con Resolución del 22 de agosto de 1982 y Expediente N 2644\/82 de la Secretaría de Agricultura, Ganadería, Pesca y Alimentación del Gobierno de la República Argentina. La Asociación Criadores de Holando Argentino (ACHA) y la Facultad de Ciencias Veterinarias de la Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), mediante, el Área de Mejoramiento Genético del Departamento de Producción Animal y el Área de Bioestadística publicaron esta revista con las evaluaciones genéticas de los reproductores Holando Argentino. Las evaluaciones genéticas fueron realizadas mediante el método BLUP, Modelo Animal. Los caracteres productivos que se evaluaron fueron: Producción de Kg. de leche, Producción de Kg. de grasa, Porcentaje de grasa, Producción de Kg. de proteína y Porcentaje de proteína. La evaluación genética por producción consideró los datos del Control Lechero Oficial disponibles desde el 1° de Enero de 1988 al 25 de Noviembre de 2014. Se utilizaron lactancias terminadas o en curso con una duración igual o superior a los 305 días, correspondientes a 465.683 vacas. El número de toros evaluados, con hijas con dato, fue 7.013. Para la obtención de los valores genéticos se tuvo en cuenta la información que aportaron los familiares, mediante la genealogía, y se incorporaron en el modelo los siguientes efectos fijos y aleatorios: Manejo, Mes de parto, Número de lactancia y edad, y Efecto ambiental permanente y Animal. Los valores genéticos se estandarizaron a una base común y se expresaron como Habilidad de Transmisión Predicha (HTP). Los caracteres morfológicos que se evaluaron correspondieron a 15 caracteres lineales principales y 5 generales: estatura, angulosidad, pecho, lomo, declive de grupa, separación de isquiones, ángulo de pezuña, calidad de hueso, patas vistas de costado, textura de ubre, inserción anterior, altura inserción posterior, ligamento medio, colocación pezones anteriores, colocación pezones posteriores, puntaje final, grupa, sistema mamario, patas y pezuñas y fortaleza lechera. La evaluación genética por morfología consideró 250.001 calificaciones realizadas por la Asociación Criadores de Holando Argentino (ACHA) mediante el Método Lineal. Se utilizaron calificaciones disponibles desde el 1° de Enero de 1988 al 25 de Noviembre de 2014. Fueron evaluados 7.611 toros. Los valores genéticos se estandarizaron a una base común y se expresaron como Habilidad de Transmisión Estandarizada (HTE); de esta manera todos los caracteres están presentados en una misma escala de variación. Los efectos no genéticos considerados en el modelo fueron: Rodeo-fecha de calificación, Número de lactancia y edad al momento de la calificación y Estado de lactancia en el momento de la calificación, incorporando al animal como efecto aleatorio. El carácter reproductivo evaluado fue Tasa de preñez de las hijas (días abiertos transformados). La evaluación genética por reproducción se basó en los datos de días abiertos del Control Lechero Oficial disponibles desde el 1° de Enero de 1988 al 25 de Noviembre de 2014. Para la obtención de los valores genéticos se utilizaron lactancias terminadas o en curso con una duración igual o superior a los 305 días, correspondientes a 373.247 vacas. El número de toros evaluados, con hijas con dato, fue 6.316. En el modelo se consideraron los efectos fijos y aleatorios Manejo, Mes de parto, Número de lactancia y edad, y Efecto ambiental permanente y Animal. Los resultados de la evaluación genética por el caracter Tasa de Preñez de las Hijas, de la misma manera que para los caracteres de producción, se publicaron como Habilidad de Transmisión Predicha (HTP) desviados de la base genética establecida. Su valor se interpreta como el porcentaje de vacas vacías que se preñan por período de 21 días. Por lo tanto, es un porcentaje que indica la habilidad de las hijas de cada toro evaluado para preñarse. Asimismo, se elaboró un índice denominado IPM. El IPM es un índice desarrollado por la Asociación Criadores de Holando Argentino con el objetivo de brindar un valor genético que combina las características de producción, tipo y reproducción de relevancia en el sistema de producción nacional. La fórmula de cálculo, con los pesos relativos, fue la siguiente:  IPM: 5 x [(33xP\/DEP)+(11xL\/DEL)+(11xG\/DEG)+(19,25xSM\/1)+(8,75xPP\/1)+(5,25xGR\/1)?(1,75xES\/1)+(10xTPH\/DET)].   Donde P: kg. de proteína. DEP: Desvío estándar para Kg. de proteína (4,6 kg.). L: kg. de leche. DEL: Desvío estándar para Kg. de leche (146,7 kg.). G: kg. de grasa. DEG: Desvío estándar para Kg. de grasa (5,0 kg.). SM: Sistema mamario. PP: Patas y pezuñas. GR: Grupa. ES: Estatura. 1: Desvío estándar de las características de tipo. TPH: Tasa de preñez de las hijas. DET: Desvío estándar para tasa de preñez de las hijas (0,49 %). Los valores mínimo, máximo, promedio y desvío estándar observados para la población de toros evaluados en los distintos caracteres fueron: -892, 601, -43±156,6 para kg de leche, -21, 23, -1,1±5,2 para kg de grasa, -0,26, 0,26, -0,01±0,05 para porcentaje de grasa, -27, 22, -1,3±5,0 para kg de proteína, -0,15, 0,27, -0,01±0,03 para porcentaje de proteína, -4,94, 3,08, -0,52±1,06 para puntaje final, -2,9, 2,5, -0,2±0,6 para tasa de preñez de las hijas y -1218, 967, -63±312,3 para IPM. Los valores para la población de hembras evaluadas fueron: -1080, 849, -85±161,1 para kg de leche, -36, 30, -2,9±5,4 para kg de grasa, -0,35, 0,39, -0,02±0,05 para porcentaje de grasa, -36, 27, -2,8±5,1 para kg de proteína, -0,26, 0,24, -0,01±0,03 para porcentaje de proteína, -6,80, 2,66, -0,67±0,98 para puntaje final, -2,2, 3,3, 0,11±0,51 para tasa de preñez de las hijas y -1689, 1698, -117±326,6 para IPM.","palabras_clave":"EVALUACIÓN GENÉTICA|MEJORAMIENTO ANIMAL|HOLANDO ARGENTINO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":592,"idioma_id":5,"titulo":"Programa  de  educación y capacitación para la conservación del arte rupestre de Sierra de la Ventana, Provincia de Buenos Aires (Argentina)","resumen":"Las investigaciones arqueológicas sistemáticas desarrolladas desde fines de la década del ´80 en el Sistema Serrano de Ventania (provincia de Buenos Aires, Argentina), permitieron detectar 32 cuevas y aleros con representaciones rupestres. Estas pinturas están siendo alteradas tanto por factores naturales como culturales, principalmente por la acción de los visitantes. A través de la implementación de campañas educativas enmarcadas en programas de preservación del arte rupestre,  se trabaja para unificar acciones entre instituciones de diferentes niveles jerárquicos. Se estableció como prioridad la divulgación de las medidas de prevención sobre los  sitios arqueológicos, la  identificación de los factores culturales que intervienen en el deterioro de estos sitios, y la elaboración de una propuesta de plan de manejo de los recursos culturales, generando estrategias de beneficio mutuo entre cultura, turismo y educación, desde la revalorización patrimonial. Entre las actividades ejecutadas pueden mencionarse las charlas informativas, la confección de folletos destinados a informar sobre la diversidad y variabilidad arqueológica de la región y la necesidad de preservar y cuidar ese patrimonio cultural, y el dictado de cursos de capacitación dirigidos a docentes, así como a guías de turismo y guardaparques; la realización de talleres plenarios con el fin de establecer criterios ecuánimes en educación y conservación, a través de políticas de consenso entre diferentes actores de los distritos de Tornquist y Saavedra.","palabras_clave":"ARTE RUPESTRE|PATRIMONIO ARQUEOLÓGICO|PROTECCIÓN|VENTANIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":614,"idioma_id":4,"titulo":"Notes on taxonomy and distribution of Myrsidea serini (Séguy, 1944) (Phthiraptera: Amblycera: Menoponidae) on Southern South American passerine birds (Aves: Passeriformes).","resumen":"Myrsidea serini (Séguy, 1944) is recorded from three different passerine hosts from Argentina and Chile: Carduelis barbata (Molina, 1782) (Fringillidae), Chrysomus thilius petersi (Laubmann, 1934) and Agelaioides badius badius (Vieillot, 1819) (Icteridae). Somatic features and body measurements from available specimens belonging to these host-populations are compared with those recorded from Old World hosts, finding only some slight differences in certain body measurements (here interpreted as correlated to differences in host sizes), but none in chaetotaxy. This species was synoxenic with Myrsidea psittaci Carriker, 1955 on C. t. petersiin at least two localities in Buenos Aires Province, Argentina. Comparative studies of external chorionic architecture of the eggs, preferential sites of oviposition, prevalence has been carried out for both species, along with some remarks concerning the still problematic species, Myrsidea argentina (Kellogg, 1906), originally recorded off Carduelis magellanica (Vieillot, 1805).","palabras_clave":"Myrsidea serini|Myrsidea argentina|Icteridae|Fringillidae|synoxeny|egg descriptions|Carduelis|Chrysomus|Agelaioides"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":620,"idioma_id":5,"titulo":"Modelos de explicación en psicología cognitiva y neurociencias","resumen":"En el presente trabajo nos proponemos analizar tres modelos de explicación propuestos  recientemente en filosofía de la psicología y filosofía de las neurociencias: la explicación uncional, el reduccionismo ruthless y el mecanicismo constitutivo, con el objetivo de determinar cuál de ellos resulta más adecuado a la hora de dar cuenta de las explicaciones en psicología y su relación con neurociencias. En esta dirección revisaremos alcances y límites de los modelos, y en qué medida estas propuestas actualizan o modifican discusiones tradicionales acerca de la autonomía\/reducción de dichas disciplinas.","palabras_clave":"AUTONOMIA|REDUCCIÓN|MECANICISMO|FILOSOFÍA DE LAS NEUROCIENCIAS|CIENCIAS COGNITIVAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":628,"idioma_id":4,"titulo":"Effects of fire on arbuscular mycorrhizal fungi in the mountain Chaco forest","resumen":"The aim of this study was to evaluate the fire effects on the AMF spore communities and soil chemical  properties as well as the existence of possible correlations between them in the Chaco Serrano Forests  of central Argentina. Our hypothesis is that the fire has a negative impact on the community of AMF  spores (i.e. density, diversity, richness and evenness) and soil chemical properties. In addition, we expect  to find a high correlation between changes in the communities of fungi and soil chemical properties. We  selected five areas in the ??Sierras Pampeanas?? mountain ranges within the Chaquean region in central  Argentina. In each of them we selected adjacent burned and unburned forest sites. Burned sites have  all the same time since fire occurrence (August?November 2009) and soil samples were collected in  autumn (April) and spring (November) in 2010. The fire events had direct negative effects on AMF spore  communities. Evenness, and notably diversity and richness of AMF spores decreased in the burned sites.  Density of AMF spores was not affected by fire. With the exception of C:N, nitrate and electrical conductivity,  soil parameters showed significant differences between burned and unburned sites. The changes in  AMF spore composition were not significantly correlated with most of the soil variables measured here.  The results of this study suggest that fire occurrence negatively affect AMF communities. These effects do  not seem to be mediated by changes in soil abiotic properties. Rather, they suggest direct effects of fire on  soil fungi.","palabras_clave":"Spores communities|Soil nutrients|Glomeromycota|Chaco Forest"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":640,"idioma_id":5,"titulo":"Educación y  religiosidad en los medios","resumen":"En Argentina con la sanción de la Ley Nacional de Educación N° 26.206 en el año 2006, las provincias tuvieron que modificar su legislación jurisdiccional. La provincia de Salta inicio en Octubre de 2008, la modificación del marco normativo con la sanción de la ley provincial N° 7.546. En este marco, uno de los aspectos más controvertidos de la nueva ley, implicaba la ratificación de la enseñanza religiosa en las escuelas salteñas.  La ley de educación provincial sostiene la enseña religiosa en escuelas primarias públicas desde finales de la década del 70. Si bien la ley de educación nacional sostiene la laicidad educativa, Salta se mantiene al margen, continuando prácticas instaladas a lo largo de su historia en materia educativa.  Nuestro trabajo analiza este debate, sobre religión y laicidad, a través de la visión de los principales medios de comunicación salteña, y como estos medios reflejaron los distintos posicionamientos, poniendo en el centro del debate la educación religiosa católica.  Fuentes como El Tribuno, El Nuevo Diario, El intransigente.com, Informatesalta.com.ar, Salta libre, y la selección de notas de estos diarios en su versión on line,  permiten pensar el rol de los medios al señalar el cruce de intereses en materia educativa.","palabras_clave":"Educación religiosa|Laicidad|Medios de comunicación"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":660,"idioma_id":4,"titulo":"S100A7 in the Fallopian tube: a comparative study","resumen":"        The oviduct is a dynamic organ in  which final gamete maturation, fertilization and early embryo development take  place. It is considered a sterile site; however the mechanism for sterility  maintenance is still unknown. S100A7 is an antimicrobial peptide that has been  reported in human reproductive tissues such as prostate, testicle, ovary,  normal cervical epithelium and sperm. The current work reports the presence of  S100A7 in the Fallopian tube and its localization at the apical surface of  epithelial cells. For comparison, porcine S100A7 is used for antibody  development and search of the peptide in reproductive tissues. Although present  in boar seminal vesicles and seminal plasma, S100A7 was not detected on female  porcine organs. Also, in contrast to human protein, porcine S100A7 did not show  antimicrobial activity under the conditions tested. Phylogenetic analyses  showed high divergence of porcine S100A7 from human, primate, bovine, ovine and  equine sequences, being the murine sequence at a most distant branch. The  differences in sequence homology, Escherichia coli-cidal activity, detectable presence  and localization of S100A7 from human and pig, suggests possible different  functions in both organisms.&nbsp;&nbsp;   ","palabras_clave":"oviducto|S100A7|psoriasina|Falopio"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":662,"idioma_id":4,"titulo":"Evaluation of the abnormal grain growth in an ASTM 213 grade T91 steel","resumen":"A study of the austenite grain growth of an ASTM A213 T91 steel held at 1050ºC for increasing times is presented. The prior austenite grain size distribution (PAGSD) at various holding times was determined using scanning electron microscopy. Taking into account the critical role played by MX precipitates (M: metal, X: C and\/or N) on grain growth, transmission electron microscopy on replicas was used to characterize the carbides or carbonitrides present during holding in austenite; thus, the particle size distribution and chemical composition of these precipitates were determined for each austenite holding time. Results  indicate a change in the chemical identity of the MX precipitates -from V-rich to Nb-rich- during the first minutes of austenite holding, the M23C6 dissolution and the further austenite grain growth in a heterogeneous mode.","palabras_clave":"FERRITIC-MARTENSITIC STEEL|GRAIN GROWTH|ELECTRON MICROSCOPY|MICROSTRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":670,"idioma_id":4,"titulo":"Dehydration affects cardiovascular nitric oxide synthases and caveolins in growing rats","resumen":"Purpose During the postnatal stage, cardiovascular nitric  oxide (NO) system and caveolins (cav) may be regulated  differentially in response to hypovolemic state induced by  water restriction. Our aim was to examine the effects of  water restriction on NO synthases (NOS) and cav in the  atria, ventricle and aorta of growing rats.  Methods Male Sprague?Dawley rats aged 25 and 50 days  were divided into (n = 15): WR: water restriction 3 days;  WAL: water ad libitum 3 days. Systolic blood pressure,  NOS activity and NOS\/cav protein levels were measured.  Results Dehydration induced a larger increase in SBP in  WR25 group. Ventricular NOS activity, endothelial NOS  (eNOS) and neuronal isoform (nNOS) of WR25 pups  were increased, and both cav were decreased. In the WR50  group, NOS activity remained unchanged. In the atria,  NOS activity, eNOS and nNOS decreased in WR25 associated  with increased cav-1; in the WR50 group, NOS activity  was increased without changes in NOS isoforms. In the  aorta of WR25, NOS activity and inducible NOS (iNOS)","palabras_clave":"CAVEOLINAS|OXIDO NITRICO |DESHIDRATACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":672,"idioma_id":5,"titulo":"?Distinción, consumo y clases sociales. Elementos para una sociología de la práctica de consumo cinematográfico?","resumen":"El presente texto se aboca a la construcción de herramientas teóricas para el abordaje del cine como práctica social, específicamente en lo referido a la dimensión del consumo. En este sentido, y desde una perspectiva anclada en la sociología de la cultura, procede al análisis de la problematización del consumo en algunos desarrollos sociológicos,  antropológicos y de comunicación contemporáneos.  Luego, pasa revista a los estudios que tomaron al cine como objeto de indagación y profundiza específicamente en aquellos que se detuvieron en el lugar del sujeto como espectador y en su función en la producción de sentido.  Finalmente, intenta proponer a partir de este estado del arte, algunas líneas y categorías teóricas que permitan la aprehensión del cine como práctica ?enclasada y enclasante?, es decir, como producción de sentido anclada y (re)productora, material y simbólica, de las posiciones en la estructura social.","palabras_clave":"Sociología|Consumos culturales|Distinción|Clases sociales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":673,"idioma_id":5,"titulo":"Volumen monográfico 'Juan Benet: Bifurcaciones, mitos y reformulaciones de la Literatura española dentro y fuera del canon'","resumen":"                    El objetivo de este monográfico es recuperar la                    figura de un autor clave de la generación del 50 en lo que concierne a                    sus aspectos teóricos sobre la novela, la memoria, la historia y la ficción.                    Algunos temas posibles (no excluyentes) comprenden:                    -el análisis del ciclo Región                    - los ensayos benetianos                    -su teatro                    -su pertenencia a la generación del 50                    -novela y subgéneros                    -metaficción, usos del mito y reformulaciones de la literatura clásica                    -Funciones ideológicas de la memoria histórica                    -postmodernismo o\/ y modernidad                    -nuevo historicismo: novela histórica, novela de la memoria                    -ruptura del canon                    -crítica del estado                    -identidad y nuevos modelos de héroes y heroínas          La edición e introducción estarán a cargo de Adriana Minardi y Jorge Machín Lucas, editora y co-editor, del presente volumen. Los críticos invitados y confirmados son: Gonzalo  Navajas, Malcolm Compitello, John Margenot, Marzena Walkowiak, Ben Frazer,&nbsp; Robert Simon y Marcelo Topuzian.            ","palabras_clave":"CANON|MITOLOGÍA|REGIÓN|ENSAYO\/NOVELA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":681,"idioma_id":5,"titulo":"Influencia sobre la productividad de diferentes densidades de raíces en plantación adulta de espárrago verde Asparagus officinalis var. altilis","resumen":"Influencia  sobre la productividad de diferentes densidades de raíces en plantación adulta  de espárrago verde Asparagus officinalis var. altilisEl espárrago es un cultivo perenne, cuya        productividad puede        ser influida por el marco de plantación utilizado. Con el        objetivo de evaluar        la productividad obtenida mediante el empleo de dos marcos de        plantación en un        cultivo iniciado por el sistema tradicional de arañas (raíces),        en su etapa        adulta, se efectúo un ensayo en la Chacra Experimental, Facultad        Agronomía,        UNCPBA (36°48´,lat.S-59°51´,long.O). El mismo se inició el        12\/09\/2003, en        bloques al azar, utilizando arañas de aproximadamente 100g, a        dos densidades:        D1= 25.000pl\/ha (1,60x0,25m) y D2: 17.857pl\/ha (1,60x0,35m),        correspondiendo la        presente evaluación al período 10\/09\/2012-16\/11\/2012. Se        efectuaron 25        cosechas, día por medio, evaluando kg totales y comerciales        (PFTyPFC), Nº        turiones totales y comerciales\/ha\/cosecha (NTTyNTC); calibres:        Jumbo:(J),        Extra-Large:(XL), Large:(L), Medium:(M), Small:(S) y        Asparagina:(A) y defectos:        turiones espigados (DE), cortos (DC), plagas (DP) y otros (DO).        Se efectuó        análisis de la varianza (ANOVA) y las medias fueron separadas        con LSD test        (P&#8804;0.05). El factor densidad influyó en el resultado productivo        obteniéndose,        en PFT: D1: 10619(a) y D2: 9625(b) kg.ha-1; en cambio, no se        encontraron        diferencias significativas en para NTT, siendo el promedio:        962375 turiones. En        PFN: la D2, superó estadísticamente a&nbsp;        D1: 8611(a) y 9968(b) kg.ha-1. NTC no presento        diferencias        significativas, siendo el promedio 468125. Respecto de los        calibres, el factor        densidad&nbsp; influyó        estadísticamente en L        (D1:94250 y&nbsp; D2:127250),        no observándose        diferencias significativas para los demás calibres estudiados,        siendo sus        promedios: J: 23625; XL: 52625; M: 162500; S: 96750; A: 14875.        Pudo observarse        también mayor número de turiones espigados en la D1: 235750(a)        respecto de D2:        166000(b). En DC, DP y DO no se encontraron diferencias        (promedios: 217375;        10625 y 59760, respectivamente). Estos resultados indican que al        llegar al 8º        año productivo la densidad comienza a influir negativamente en        la productividad        comercial, por efecto de la mayor competencia entre plantas.","palabras_clave":"DENSIDAD|PRODUCCION|TURIONES|CALIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":692,"idioma_id":4,"titulo":"Pararotor dynamics: center of mass displacement from the blade plane. Analytical approach","resumen":"The pararotor is a biology inspired decelerator device based on the autorotation of a rotary wing whose main purpose is to guide a load descent into a certain atmosphere. This paper focuses on a practical approach to the general dynamic stability of a pararotor whose center of mass is displaced from the blade plane. The analytical study departs from the motion equations of pararotor flight, considering the center of mass displacement from the blade plane, studied over a number of simplifying hypotheses that allows determining the most important influences to flight behavior near equilibrium. Two practical indexes are developed in order to characterize the stability of a pararotor in terms of geometry, inertia and the aerodynamic characteristics of the device. Based on these two parameters, a stability diagram can be defined upon which stability regions can be identified. It was concluded that the ability to reach stability conditions depends mainly on a limited number of parameters associated with the pararotor configuration: the relationship between moments of inertia, the position of the blades, the planform shape (associated to blade aerodynamic coefficients and blade area), and the vertical distance between the center of mass and the blade plane. These parameters can be evaluated by computing practical indexes to determine stability behavior.","palabras_clave":"decelerador|alas rotantes|dinámica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":699,"idioma_id":4,"titulo":"Behavior of Impatiens wallerana Hook. F in alternative pot substrates: mechanisms involved and research perspectives","resumen":"The approach to select new growing media, has been focused on selecting materials only from the physical point of view. The objective of this study was to describe the physiological mechanisms involved in I. wallerana growth when cropped on a broad range of growing media created from alternative components. Results showed a close relationship between I. wallerana growth and fine particle size at the beginning of the experiments. Shoot fresh weight was determined mainly by the root system size. There were small differences in the relative growth rate (RGR) between the control substrate and the thirty alternative substrates tested. The lower RGR values resulted from a decrease in the net assimilation rate and the leaf area ratio. The mechanism involved would be associated with a change in photosynthate partitioning, which favored root growth. A close relationship between growth (as total dry weight) and nitrogen content was found as well.","palabras_clave":"ornamental plants|peat|river waste|nitrogen"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":712,"idioma_id":5,"titulo":"Caracterización histológica del tejido conectivo sometido a fuerzas ortodóncicas.","resumen":"La investigación histológica ha permitido establecer conexión entre los hallazgos histológicos y el planeamiento profiláctico o terapéutico sirviendo  de sustento como principio básico para la toma de decisiones en la clínica. Este proyecto de tipo descriptivo-analítico tiene por objeto  describir las características del tejido conectivo periodontal y de los fibroblastos sometidos a fuerzas ortodóncicas mediante microscopía óptica con técnicas de coloración histológica convencionales (Hematoxilina-Eosina).  Las muestras de tejido periodontal se obtuvieron de pacientes que concurrieron al Servicio de Ortodoncia de la Facultad de Odontología de la UNNE y en los cuales como parte del tratamiento está indicada la extracción dentaria. Las  fuerzas aplicadas fueron de tipo leve con arcos iniciales elásticos, arco de Niti 0,12; o arco de acero trenzado, twinflex o coaxial. El tiempo de aplicación de la fuerza varió entre 15 y 30 días. Los preparados histológicos obtenidos a partir de las muestras evidencian a los fibroblastos dispuestos paralelos a los haces de fibras lo que hace suponer su intensa actividad remodeladora  durante la aplicación del movimiento ortodóncico manteniendo su morfología celular. Hay presencia de aislados focos de hemorragia y focos inflamatorios leves.","palabras_clave":"ORTODONCIA|CÉLULAS DEL TEJIDO CONECTIVO|PERIODONTO|HISTOMORFOLOGÍA CELULAR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":727,"idioma_id":5,"titulo":"Producción Participativa del Hábitat Popular. Aportes metodológicos a partir del estudio del Gran San Miguel de Tucumán 2000-2010","resumen":"La investigación busca caracterizar el proceso de producción participativa del hábitat popular tomando como caso la conformación del aglomerado Gran San Miguel de Tucumán. Dicha temática requiere del estudio de políticas públicas de producción del hábitat, para comprender las lógicas de acción naturalizadas en estos procesos.  Para esto analiza la conformación del área metropolitana de Tucumán, reconociendo en la dinámica de los grupos vulnerables las posibles formas de condicionamiento a la participación en la producción del hábitat.   Se toman dos casos concretos de producción participativa del hábitat en barrios populares, uno de gestión estatal, y otro autogestionado.  De esta forma es posible detectar coincidencias y diferencias entre la teoría de la participación y las prácticas concretas institucionalizadas en el medio, reconociendo los obstáculos que condicionan la concreción de la práctica en un proceso participativo. Finalmente se elabora una categorización de variables que implican diferentes instancias de participación.","palabras_clave":"Participación|Expansión Urbana|Hábitat popular"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":739,"idioma_id":5,"titulo":"Docudrama y autoficción en el teatro argentino de la posdictadura","resumen":"En el marco de la posdictadura, la dramaturgia argentina ha desarrollado múltiples lineamientos estéticos. Entre otros, podemos destacar el pasaje de las experiencias del docudrama hacia las escenas sostenidas en los relatos autoficcionales, esto último, entendido como un proceso poético específico y evidenciado en diversas regiones del país. Para comprender esta estrategia poética, en este artículo abordaremos tres casos de estudio de regiones distintas: CABA, NOA y Patagonia Argentina. Este trabajo es la ampliación y desarrollo exhaustivo de la ponencia dictada en las I Jornadas Internacionales de Narrativa Actual y ha sido seleccionado por un comité evaluador internacional para integrar el número monográfico de la revista Pasavento, dedicado íntegramente al tema.","palabras_clave":"DOCUDRAMA|AUTOFICCIÓN|TEATRO ARGENTINO|POSTDICTADURA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":744,"idioma_id":5,"titulo":"Marcelino Ugarte. Sus pretensiones presidenciales y el intento de restablecer la hegemonía política de Buenos Aires.","resumen":"El gobierno de Marcelino Ugarte  en la provincia de Buenos Aires debe ser estudiado porque, en forma muy marcada, fue un claro intento de un gobernador bonaerense de romper con el presidente y tratar desde La Plata de volver a colocar a la situación provincial en clara posición de incidir en la política nacional y simultáneamente, de preparar el campo para poder así llegar a la presidencia de la Nación.   Es claro que la dirigencia política provincial de Buenos Aires perdió protagonismo desde 1880 en adelante; los intentos esporádicos de participación activa o de pretensiones presidenciales, tales como los de Rocha y Costa, fueron rápidamente abortados. Así, la provincia vivió en sujeción directa de los presidentes de turno y sus gobernadores llegaron a serlo tras el consentimiento de aquellos. El presente trabajo ha de estudiar el proceso que se inicia con la llegada al gobierno de Ugarte y se frustra con la renuncia al cargo del presidente Manuel Quintana ocurrido en enero de 1906.  Palabras clave: Ugarte - Política ? Elecciones - Partidos políticos","palabras_clave":"Ugarte|Política|Elecciones|Partidos políticos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":752,"idioma_id":5,"titulo":"Auditoría Financiera en Entornos de Computación en la Nube: Revisión del Estado del Arte.","resumen":"El uso de la Computación en la Nube (CN) en las organizaciones representa un  nuevo  desafío  para  los  auditores  financieros,  dado  que  posee particularidades  que  deben  ser  revisadas  por  su  impacto  en  el  encargo  de auditoría. El propósito de este trabajo consiste en realizar una revisión de  la literatura  y  normativa  vigente  en  la  República  Argentina  respecto  de  los efectos  de  la  CN  sobre  la  auditoría  financiera  y  plantear  retos  de investigación  que  resulta  importante  afrontar.  Al  respecto  se  abordan aspectos  de  la  auditoría  considerados  relevantes  en  este  entorno:  el conocimiento  del  cliente;  la  identificación  y  evaluación  de  riesgos;  la evaluación  del  sistema  de  control  interno;  las  características  específicas  de las  evidencias  y  las  habilidades  y  competencias  requeridas  al  auditor  y  el uso de especialistas. La investigación de estas temáticas permitirá fortalecer los  conocimientos  en  esta  área  y  contribuir  en  la  elaboración  de  normas  y recomendaciones que sirvan de guía para los  profesionales en el ejercicio de su labor.","palabras_clave":"AUDITORÍA FINANCIERA |ENTORNOS DE COMPUTACIÓN EN LA NUBE |RIESGOS  DE  LA  COMPUTACIÓN  EN  LA  NUBE  |ASPECTOS  DE  AUDITORÍA POTENCIALMENTE AFECTADOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":787,"idioma_id":5,"titulo":"Las deserciones en el Ejército Auxiliar del Perú durante las Guerras de Independencia en el Río de la Plata, 1810-1820. Una aproximación cualitativa.","resumen":"Los sucesos de mayo iniciaron el proceso revolucionario que iba a culminar con al declaración de Independencia del Virreinato del Río de la Plata de la corona española. En este contexto, el Ejército Auxiliar del Perú, se transformó en uno de los principales instrumentos con los que contó la elite revolucionaria para consolidarse en el poder. El presente trabajo pretende abordar uno de los fenómenos más recurrentes dentro del ejército, las deserciones, a partir de las Memorias y Autobiografías de aquellos que se desempeñaron como oficiales en dicho ejército entre 1811 y 1820","palabras_clave":"Memorias|Deserciones|Oficiales|Tropas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":792,"idioma_id":5,"titulo":"De la revolución a la nano-intervención: tonos, inflexiones y acentos en la escena teórica contemporánea?","resumen":"El artículo plantea una hipótesis respecto de los cambios de tonos, inflexiones y acentos que se observan en la escena teórica contemporánea. Una variación que, lejos de una claudicación, supone una mirada atenta a los cambios de la cultura y, en particular, a cómo la literatura registra estas transformaciones. Se sigue para ello la producción de Julia Kristeva, Tzvetan Todorov y Jacques Derrida en diálogo con la de Avital Ronell. Finalmente se plantea una pregunta respecto de por qué las categorías que surgen con sello latinoamericano se inscriben sin dudar en la crítica pero no en el campo teórico: una pregunta que recobra valor en el marco general del viraje de la escena teórica internacional antes descripto. Se trabaja para este último punto con algunos ejemplos (regionalismo no regionalista de Beatriz Sarlo, nacionalismo no nacionalista de María Teresa Gramuglio, cuento de Josefina Ludmer y de Rossana Nofal, populismo de Ernesto Laclau, acefalía de Raúl Antelo, ciudad letrada de Ángel Rama, transculturación de Fernando Ortiz y de Ángel Rama, modernidad periférica de Sarlo, operaciones de Jorge Panesi y política de Eduardo Rinesi.","palabras_clave":"REVOLUCIÓN |NANOINTERVENCIÓN|TONOS|ESCENA TEÓRICA CONTEMPORÁNEA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":801,"idioma_id":5,"titulo":"Combustión de vinaza con materiales celulósicos en calderas bagaceras","resumen":"-\tFranck Colombres F. J., M.A. Golato, E. Feijóo, W.D. Morales, M. Octaviano y D.Paz.  Combustiónde vinaza con materiales celulósicos en calderas bagaceras. Presentado RIAT julio 2013.","palabras_clave":"calderas|residuos insdustriales|combustion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":808,"idioma_id":5,"titulo":"Política, medio ambiente y democracia, un debate que apenas comienza","resumen":"Ensayo acerca de la relación entre sociedad ambiente y ciudadania","palabras_clave":"Historia|Ambiente"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":812,"idioma_id":5,"titulo":"La Actitud de los Docentes frente a las Tecnologías de la Información y la Comunicación.  El caso de la Facultad de Ciencias Económicas de la Universidad Nacional de Jujuy","resumen":"La incorporación de las tecnologías digitales en los contextos educativos es un tema que, desde más de una década forma parte de la agenda y el debate de los foros académicos. la temática conlleva a repasar todas y cada una de las variables que intervienen en el acto educativo. Los docentes, como parte fundamental del mismo, se enfrentan al desafío de modificar los paradigmas tradicionales que sustentan la forma de transmitir el conocimiento del cual disponen.Precisamente, el presente trabajo tiene como objetivo presentar los resultados del análisis efectuado sobre los 'docentes'.&nbsp;Específicamente, el proceso indagatorio abordó, en una primera instancia, la denominación de las actitudes de los mismos respecto al uso de las Tecnología de la&nbsp;Información y la Comunicación (TICs) en un contexto educativo particular. en la segunda etapa, se indagó sobre las capacitación pedagógica-tecnológica de los docentes en el manejo de las aulas virtuales y la implementación de las mismas en las diferentes cátedras de las carreras de Contador Público y Licenciatura en Administración (Facultad de Ciencias Económicas-FCE- de la Universidad Nacional de Jujuy -UNJu) y la Licenciatura en Economía ( Ciclo de articulación con la Facultad de Ciencias Económicas de la Universidad Nacional de Tucumán)&nbsp;","palabras_clave":"Aulas virtuales|Actitud |Docentes|Ciencias Económicas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":824,"idioma_id":5,"titulo":"Mucho más que sólo aprovisionamiento lítico: Actividades en canteras y prácticas sociales en las fuentes de Pampa Oeste, Quebrada Seca y Punta de la Peña (Antofagasta de la Sierra, Catamarca)","resumen":"En este trabajo se abordan las  canteras y talleres asociados de Punta de la Peña, Quebrada Seca y Pampa Oeste,  en Antofagasta de la Sierra (Catamarca), con el objeto de explorar la  multiplicidad de actividades y prácticas sociales involucradas en la  utilización de dichas fuentes. Se trata de tres fuentes de vulcanitas  intensamente explotadas a lo largo de la secuencia prehispánica del área.De esta forma, se toma a las  canteras no sólo como un depósito de rocas útiles para la subsistencia, sino  como un escenario social donde confluyen y se materializan diferentes prácticas  y expresiones sociales. Así, a la explotación de las rocas se suman toda una  serie de tareas vinculadas, por ejemplo, con prácticas de enseñanza\/aprendizaje  y actividades de subsistencia, procesamiento\/consumo y rituales. Esta aproximación considera a las  canteras desde una perspectiva orientada a abordar las elecciones y prácticas  llevadas a cabo por los agentes en contextos de aprovisionamiento, partiendo de la base de que estos actúan desarrollando una diversidad de actividades conectadas en torno a la apropiación de un recurso específico.","palabras_clave":"CANTERAS|APROVISIONAMIENTO LÍTICO|PRÁCTICAS SOCIALES|HABITUS|PUNA ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":845,"idioma_id":5,"titulo":"Publicación de la Maestría en Bioseguridad","resumen":"500 EJEMPLARES","palabras_clave":"BIOSEGURIDAD|ARGENTINA|VETERINARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":848,"idioma_id":5,"titulo":"Procesos analíticos para la elaboración del perfil de mortalidad de las series esqueletarias del punto arqueológico La Caridad (Área Fundacional de Mendoza).","resumen":"El perfil de mortalidad constituye un paso básico para los posteriores estudios bioarqueológicos. En este trabajo se presentan los procedimientos metodológicos seguidos para su elaboración, ya que se trata de una estructura de entierro compleja por presentar inhumaciones primarias y secundarias. Se analizó una muestra de 34 individuos recuperados en el  templo colonial La Caridad correspondiente a los siglos XVII-XIX. Por un lado se presenta la metodología utilizada para las determinaciones sexuales y de edad de muerte y por otro, los estudios de error intra e interobservador de las observaciones como parte del proceso de construcción del perfil de mortalidad de la muestra. Los estudios de error, la puesta prueba de diferentes metodologías y de distintos marcadores óseos y dentales constituyen una base metodológica para conocer el error intrínseco de las observaciones y la variabilidad en la exactitud y eficacia de determinadas variables anatómicas para realizar las determinaciones de sexo y edad. Este conocimiento resulta relevante para los posteriores estudios sobre conjuntos esqueletales desagregados  correspondientes a los entierros secundarios propios de estos templos.","palabras_clave":"metodologia|entierros primarios|entierros secundarios|perfil de mortalidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":900,"idioma_id":4,"titulo":"Barriers and the limits of neodevelopmentalism: Lessons from Argentina's experience, 2003-2011","resumen":"In this paper we study the development of contradictions, barriers and limits in Argentina?s new pos-neoliberal hegemonic project: neodevelopmentalism. In the following section we will briefly describe the constitution and basic foundations of this new process. We?ll show how neodevelopmentalism has worked as an attempt by dominant fractions of capital to take advantage of the neoliberal legacy while at the same time attempting to surpass its limitations. Later on, we present the basic contractions and barriers of this new project as they manifest themselves as forms of class struggle. Finally, we?ll discuss how neodevelopmentalism in Argentina manages the impact of the first crisis in world capitalism in the 21st century. Finally we present some conclusions.","palabras_clave":"neodesarrollismo|limites|argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":909,"idioma_id":5,"titulo":"Una cartografía de los afectos en el arte argentino contemporáneo","resumen":"Con cierta inspiración del Atlas of Emotion en el que Giuliana Bruno  traza un ?viaje emocional? a través de la arquitectura y el arte a fin de construir una geografía de la intimidad, este texto pretende configurar un mapa de las emociones involucradas en el contexto actual desde su superficie estética. Para ello, parece inevitable establecer algo así como un punto cero del presente: hay que comenzar con el 2001.   La crisis del 2001 revela un ?desfondamiento? del Estado ?como propone Ignacio Lewkowicz en Pensar sin Estado. La subjetividad en la era de la fluidez -, que los artistas acompañaron con estrategias que configuran las sensibilidades estéticas y políticas de la actualidad. En la poscrisis se vuelve sobre la última dictadura, pero se piensan también las sexualidades, las reivindicaciones políticas y la experimentación artística como modo de habitar el mundo. La puesta en evidencia de la descomposición de una cierta política como soporte y referente de lo colectivo compele al arte a recuperar lo público como espacio contradictorio y a hacer suya la promesa de un agonismo estético.","palabras_clave":"ARTE ARGENTINO CONTEMPORÁNEO|GIRO AFECTIVO|ESTUDIOS DE GÉNERO|ATLAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":920,"idioma_id":4,"titulo":"LEGUME (MIMOSOIDEAE) FOSSIL WOODS FROM THE LATE MIOCENE (SALICAS FORMATION) OF NORTHWESTERN ARGENTINA","resumen":"ABSTRACT  ?  Six pieces of fossil wood from the late Miocene of northwestern Argentina (Salicas Formation) are described. They are all assigned to  Prosopisinoxylon anciborae Martínez based on the presence of the following diagnostic characters: vessels of two distinct diameter  classes, simple perforation plates, vestured pits, abundant axial parenchyma and wide rays. Significant anatomical variation exists among specimens but they are regarded as a single taxonomic unit because usually a gradual transition is observed among most variable   characters. A principal component analysis (PCA) was performed and results agree with the taxonomic placement. The analysis also shows discrete clusters of other related Leguminosae fossil-genera. Wood anatomy suggests a warm and dry climate for  the zone supporting   previous studies.","palabras_clave":"Fabaceae|La Rioja|Prosopisinoxylon anciborae| PCA."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":927,"idioma_id":4,"titulo":"Adaptive Simulation of the Internal Flow in a Rocket Nozzle","resumen":"This work is a first step in the understanding of the interaction process between internal shock waves and the flow transition inside of a rocket nozzle that develops during the engine start-up phase or when the nozzle is operated at overexpanded conditions. In many cases, this transition in the flow pattern produces side loads in the nozzle due to an asymmetric pressure distribution on the wall, being harmful for the rocket?s integrity. To understand this phenomenon, a numerical simulation is performed by solving the three-dimensional Euler equations on unstructured tetrahedral meshes. With this model the computational cost to solve the equations significantly increases, therefore parallel processing is required. Also, an unsteady h-adaptive refinement strategy is used jointly with a Streamline Upwind Petrov-Galerkin and a discontinuity capturing scheme, both to keep the size of the fluid flow problem bounded and to sharply resolve the shock wave pattern. The mesh adaptation strategy is introduced. Since its performance is a major concern in the solution of unsteady flow problems, some implementation issues about the data structure chosen to represent the mesh are discussed. Average pressure distributions computed at the wall and the axis of the nozzle for various pressure ratios are analyzed based on experimental and numerical results from other authors.","palabras_clave":"Mecánica Computacional"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":932,"idioma_id":5,"titulo":"Deíxis y contacto en la región del Gran Chaco: los demostrativos en las lenguas guaycurúes y mataguayas","resumen":"Las lenguas guaycurúes y mataguayas (Gran Chaco) constituyen casos interesantes y tipológicamente inusuales por la complejidad de sus sistemas de demostrativos. Si bien la deíxis es un fenómeno bien conocido, su combinación con clasificación nominal, tiempo, modo y evidencialidad no es común en la mayoría de las lenguas del mundo.     En la familia guaycurú los demostrativos son de naturaleza mixta, en tanto amalgaman deíxis con forma y posición del referente (parado [vertical]; acostado [horizontal] y sentado [tridimensional]), contribuyendo así a la clasificación nominal en estas lenguas. Las lenguas de la familia mataguaya presentan sistemas similares, aunque en ellas la deíxis se combina con rasgos como percepción táctil y visual, así como movimiento, entre otros. En las lenguas de ambas familias los demostrativos contribuyen también al señalamiento pragmático del tiempo, el modo y la evidencialidad, no necesariamente codificadas en el predicado.  El trabajo muestra las semejanzas entre los sistemas comparados y propone que ellas no pueden explicarse por causas universales o mera coincidencia, sino que el contacto juega allí un papel importante. Por otra parte, aun cuando la hipótesis de una relación genética entre ambas familias sea razonable, esta no parece suficiente para explicar las similitudes en los sistemas de demostrativos, que van más allá de las formas presumiblemente cognadas.","palabras_clave":"SISTEMAS DE DEMOSTRATIVOS|DEÍXIS|GRAN CHACO|LENGUAS GUAYCURÚES Y MATAGUAYAS|LENGUAS EN CONTACTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":933,"idioma_id":5,"titulo":"Ética, cuerpo y subjetividad actual.  Algunas perspectivas sobre el cruce discursivo entre la Bioética y las Ciencias de la Salud.","resumen":"Este artículo presenta entre sus objetivos principales conocer el modo en que se configura el campo de la Bioética actual, como se despliegan los significantes que atraviesan dicho campo y cómo se entrelazan y yuxtaponen con otros discursos. Así, se trabajan los resultados obtenidos en cuanto a las perspectivas del concepto de Bioética y su entrecruzamiento discursivo con el campo de la Medicina y de la Salud Psíquica. Se plantea, desde este lugar, la necesidad de pensar los diferentes dilemas bioéticos atendiendo fundamentalmente a la dimensión del sujeto.Para esto se elaboró una base teórica sustentada en algunas categorías que sostuvieron la investigación (Ética, Cuerpo, Hipermodernidad, Biopolítica, Posthumanismo). Dicho marco se produjo basado en pensadores de la Filosofía, la Sociología y la Historia, entre otras disciplinas, para desde allí establecer cruces interdisciplinarios con otras perspectivas, entre ellas, el Psicoanálisis. En segunda instancia, se analizaron diversos dominios discursivos de la ciencia y la cultura actual, como son la Medicina, la Psicología, el Psicoanálisis, la Prensa, el Arte, el Derecho, en un corpus configurado a tal fin, siendo el método utilizado el Análisis del Discurso sobre una selección de materiales significativos.","palabras_clave":"Biopolitica|Bioetica|Cuerpo|Inconciente"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":950,"idioma_id":5,"titulo":"Algunos aportes para entender y prevenir la violencia escolar en adolescentes","resumen":"Esta presentación propone ciertos vectores para un mayor entendimiento de la violencia escolar en adolescentes y su prevención. Es preciso aclarar que sólo tomamos algunos de todos los posibles y nos enfocamos en el ámbito escolar","palabras_clave":"VIOLENCIA ESCOLAR|ADOLESCENCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":951,"idioma_id":5,"titulo":"Cantar: resplandores tardíos de la civilización y la barbarie.","resumen":"La voz nos permite hablar. Y cantar. Es capaz de convertirse en susurro o alarido. Callamos con la voz. No es posible concebir el pensamiento sin la resonancia tangible de la palabra. Lo primero es el llanto. Se llora con la voz. Y ese gemido es la prueba irrefutable que parteros y obstetras exigen en cada alumbramiento. Escuchamos a nuestros niños en soliloquios y laleos que se sitúan entre el canto y el monólogo. Reímos a carcajadas que se contagian a partir de su sonoridad. La emisión integra parte de las experiencias más íntimas e intrínsecas de la vida como un rasgo esencial  en las relaciones sociales, en el arte y en la comunicación, desde los lindes de la historia.","palabras_clave":"CANTO|CIVILIZACIÓN|BARBARIE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":952,"idioma_id":4,"titulo":"A Robust Adaptive Path-Following Controller for a Robotic Wheelchair","resumen":"This article proposes a path-following controller for robotic wheelchairs (RW) used to transport people suffering of severe muscular diseases, taking into account velocity bounds and dynamic effects. A parameterized dynamic model, which considers the person on board the RW, is used. The model parameters normally change, generating structured uncertainties. Moreover, the dynamic model is proposed under some simplifications, introducing unstructured uncertainties. Finally, time-varying dynamics, caused basically by user movements, are also considered. Hence, the dynamic controller proposed is adaptive and robust. Experimental and simulation results show the effectiveness and the good performance of the proposed control system.","palabras_clave":"robotic wheelchair|path following|dynamic uncertainties|robust adaptive control"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":957,"idioma_id":4,"titulo":"Linking thermo-tolerance of Chagas disease vectors, Rhodnius prolixus and Triatoma infestans, to their geographical distribution","resumen":"Understanding  the relationship between geographic range limits and physiological traits of  vector species is under increasing demand to predict the potential effects of  global warming, not only in terms of geographic distribution of vector species but  also in terms of the risk of disease transmission. Like in many other insect  species, the geographical distribution of Chagas? disease vectors is affected  by temperature. This study examines, for the first time, the relationship  between the limits of geographic distribution and thermo-tolerance of the most  important vectors of Chagas disease, Triatoma infestans in southern South  America and Rhodnius prolixus in northern South America and Central America, to  test the climatic variability hypothesis (CVH). We applied species distribution  modeling (SDM) using bioclimatic variables and identified the most important  limiting factors of the habitat suitability. Then, we measured and compared:  the critical thermal maximum (CTmax) and the upper lethal temperature (ULT)  (measured by thermo-limit respirometry), chilled coma recovery (i.e. the time  to recovery from 4 h at 0°C) and the critical thermal minimum (CTmin). For both  species the minimum temperature of the coldest month was the most important  abiotic factor restricting their geographic distribution. By taking a  correlative approach and testing predictions with thermal tolerance traits, it  was possible to explain the southern limit distribution for both species in  terms of physiological constraints. The greater temperature tolerance of T.  infestans compared to R. prolixus supports the CVH.&nbsp; ","palabras_clave":"CHAGAS DISEASE|ECOPHYSIOLOGY|RHODNIUS PROLIXUS|TRIATOMA INFESTANS|THERMAL LIMITS|BIOGROGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":981,"idioma_id":5,"titulo":"La Odontología Basada en la Evidencia: autoaprendizaje de la farmacología centrado en la asociacion de contenidos y la construcción del conocimiento.","resumen":"El paradigma basado en la evidencia tiene como modelo jerárquico a la información basada en la evidencia, que consiste en la revisión sistemática en la que se lleva a cabo una exhaustiva búsqueda bibliográfica para seleccionar sólo una cantidad limitada de estudios valiosos. Objetivo: Desarrollar competencias en los alumnos que tiendan a la construcción del conocimiento a través de la odontología basada en la evidencia para resolver problemas relacionados al uso racional de medicamentos. Materiales y métodos: Se realizó un estudio descriptivo observacional, sobre una muestra constituida por 150 alumnos que cursan actualmente la asignatura Farmacología y Terapéutica B. Los alumnos realizaron un autoestudio guiado, con búsquedas en bases de datos informatizadas, eligiendo temas correspondientes a la Unidad de Dolor. En la actividad de cierre se presentaron las producciones en formato póster. Se realizó una encuesta anónima autoadministrada con preguntas estructuradas para evaluar la actitud de los estudiantes frente a esta nueva modalidad de trabajo.  Y otra encuesta de opinión sobre aspectos positivos y negativos de esta forma de cursado. A partir de las mismas se analizaron las frecuencias de respuestas. Resultados: Los datos obtenidos fueron: al 52,23% de los alumnos le resultó una actividad muy interesante; al  56,71% le pareció buena la metodología aplicada; el  80,59% relató que todos los integrantes del grupo participaron, mientras que el 16,41  % afirmó que sólo algunos lo hicieron; el 83,28% consideró que esta actividad se podría aplicar a todas las unidades de la asignatura; el 76,26% piensan que podría aplicarse la metodología en otras asignaturas de la carrera. Conclusiones: De los resultados obtenidos, concluimos que esta propuesta pedagógica brindaría al alumno las herramientas necesarias para tomar decisiones terapéuticas en el marco de la odontología basada en la evidencia con el objeto de contribuir al uso racional de medicamentos.","palabras_clave":"Odontología basada en la evidencia|Uso racional de medicamentos, |Enseñanza de la Farmacología"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":988,"idioma_id":5,"titulo":"La educación ambiental (EA) como 'saber maldito'. Apuntes para la reflexión y el debate","resumen":"Este artículo se propone reflexionar y debatir sobre el saber ambiental como una praxis subvertidora de los contextos educativos institucionalizados y de las prácticas educativas instituidas; un nuevo lenguaje para comprender el mundo, una nueva alfabetización. Estos atributos convierten a la Educación Ambiental (EA) en un 'saber maldito' ubicado en las fronteras institucionales de los sistemas educativos. Concebir y esforzarnos en crear escenarios de transformación fuerte, dentro mismo de los contextos educativos instituidos lleva a practicar lo instituyente para destituir lo hegemónico, privilegiando la duda y anteponiendo las preguntas a las certezas, comunicando la incertidumbre y debatiendo la complejidad. Las TIC, por otra parte, pueden desde un sentido crítico potenciar la EA. Destacar el valor que aún tienen los espacios educativos públicos estatales para sembrar las semillas de una rebelión desnaturalizadora de las visiones hegemónicas de mundo, es por fin, la mayor de las deudas que quienes nos sabemos educadores ambientales debemos asumir.","palabras_clave":"EDUCACIÓN AMBIENTAL|NUEVAS ALFABETIZACIONES|TIC|SABER AMBIENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1002,"idioma_id":4,"titulo":"TOXICOLOGICAL AND BIOCHEMICAL RESPONSE OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA AFTER EXPOSURE TO DELTAMETHRIN","resumen":"BACKGROUND The chemical control of the Chagas disease vector Triatoma  infestans is endangered by the emergence of pyrethroid resistance. An effective  alternative control tool is the use of the entomopathogenic fungus Beauveria bassiana.  The effect of deltamethrin on fungal growth, gene expression and enzyme activity in  relation to detoxification, antioxidant response and oxidative stress levels was studied to  evaluate fungal tolerance to deltamethrin.   RESULTS The mean inhibitory concentration (IC50) was 50 &#956;g deltamethrin\/cm2.   Cytochrome P450 genes were differentially expressed; cyp52X1 and cyp617N1  transcripts were > 2-fold induced, followed by cyp655C1 (1.8-fold). Minor effects were  observed on genes encoding for other P450s, epoxide hydrolase and glutathione S5  transferase (GST). Superoxide dismutase (SOD) genes showed induction levels &#8804; 2,  catalase (CAT) and glutathione peroxidase genes were also induced ~ 2-3-fold and < 2-  fold, respectively. The activities of enzymes participating in the antioxidant defense  system and phase II detoxification were also evaluated; SOD, CAT and GST activity  showed significant differences with deltamethrin concentration. Lipid peroxidation  levels and free proline content were also altered.  CONCLUSIONS B. bassiana GHA can be used combined with deltamethrin without  significant metabolic detrimental effects. This combination will help optimizing the  benefits and increasing the efficacy of vector control tools.","palabras_clave":"ENTOMOPATHOGENIC FUNGI|OXIDATIVE STRESS|ANTIOXIDANT RESPONSE|PYRETHROID DETOXIFICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1003,"idioma_id":4,"titulo":"Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development","resumen":"In this work, histological and histochemical features of the larval digestive system of Argentine anchovy Engraulis anchoita were described. Structural changes during ontogenetic development were also characterized, and comparisons between the beginning and the end of larval development weremade. Histological sections of larvae were subjected to histochemical and routine histological techniques to  localize and differentiate glycoproteins (GPs). Both at an early and a late larval stage, the oesophageal goblet cells reactedmore intensely than those of the rest of the digestive tract, and only the oesophagus exhibited metachromasia with toluidine blue techniques at different pHs, thus revealing diverse GPs at different concentrations. The GPs histochemical composition in the intestine varied with the developmental stage and the intestinal zone. The absence of goblet cells characterized the foregut; however, they started differentiation at an advanced stage in the midgut. These cells could be detected in the hindgut both at the beginning and at the end of development.The attached glands showed a varied glycoprotein composition. The digestive tract of E. anchoita presented a high level of complexity, related to the multiple functions of mucus in the digestive tract, such as lubrication, protection, antimicrobial function and ionic and osmotic regulation.","palabras_clave":"ENGRAULIS ANCHOITA|HITOCHEMISTRY|GLYCOPROTEINS|DIGESTIVE SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1004,"idioma_id":4,"titulo":"Preclinical development of novel Rac1-GEF signaling inhibitors using a rational design approach in highly aggressive breast cancer cell lines","resumen":"Rho GTPases play a key role in the regulation of multiple essential cellular processes, including actin dynamics, gene transcription and cell cycle progression. Aberrant activation of Rac1, a member of Rho family of small GTPases, is associated with tumorigenesis, cancer progression, invasion and metastasis. Particularly, Rac1 is overexpressed and hyperactivated in highly aggressive breast cancer. Thus, Rac1 appears to be a promising and relevant target for the development of novel anticancer drugs. We identified the novel Rac1 inhibitor ZINC69391 through a docking-based virtual library screening targeting Rac1 activation by GEFs. This compound was able to block Rac1 interaction with its GEF Tiam1, prevented EGF-induced Rac1 activation and inhibited cell proliferation, cell migration and cell cycle progression in highly aggressive breast cancer cell lines. Moreover, ZINC69391 showed an in vivo antimetastatic effect in a syngeneic animal model. We further developed the novel analog 1A-116 by rational design and showed to be specific and more potent than the parental compound in vitro. We also showed an enhanced in vivo potency of 1A-116 analog. These results show that we have developed novel Rac1 inhibitors that may be used as a novel anticancer therapy","palabras_clave":"Breast Cancer|Docking |Rac1 inhibitor|Rational Design"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1006,"idioma_id":4,"titulo":"Osmotic dehydration dynamic of common carp (Cyprinus carpio) fillets using binary and ternary solutions","resumen":"In this work, water activity (a w), moisture loss, and salt uptake as mass transfer in osmotic dehydration (OD) of Cyprinus carpio fillets were analyzed. Two highly concentrated solutions were used for the OD: binary (NaCl-water) and ternary (NaCl-sucrose-water) solutions at 20°C. Both solutions had the same NaCl concentration, but the second also had sucrose (2:1) which improved water loss and reduced NaCl gain. Among the OD models analyzed, Peleg´s equation presented best fit with the ternary solution (R2&gt;0.900), while the Zugarramurdi and Lupín did so for the binary solution treatment (R2&gt;0.950). Page´s model presented good fit for water release in both cases (R2&gt;0.950).","palabras_clave":"OSMOTIC DEHYDRATION|COMMON CARP|MASS TRANSFER|EMPIRICAL MODELS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1007,"idioma_id":4,"titulo":"Comparative study of the thermal fluctuations effects on the classical Stoner-Wohlfarth and analytical vector hysteron models","resumen":"&nbsp;In this work we present a comparative study of the classical Stoner Wohlfarth model and the analytical vector hysteron model. We analyze the hysteretic behavior description when the thermalfluctuations are included. We found a different behavior in the evolution of the coercivefield as function of temperature for the vector hysteron model when the anisotropy easy axis is non-parallel to the magnetic field orientation. At low temperatures a plateau is observed as consequence of the switchingfield behavior. At high temperature both models give identical description of the magnetization behavior, which merges with the superparamagnetic approximation.&nbsp;","palabras_clave":"STONER-WOHLFARTH MODEL|ANALITICAL VECTOR HYSTERON MODEL|HYSTERETIC BEHAVIOR MODEL|MAGNETIZATION INVERSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1008,"idioma_id":4,"titulo":"Osteometric Analysis of South American Sea Lions (Otaria flavescens) Pups From Patagonia. An Assessment of Their Use as Indicators for Seasonality in Archaeological Sites","resumen":"Zooarchaeological analyses of pinniped remains have been scarce in South America because of lack of specific studies of species determination and estimation of age and sex. Nonetheless, the presence of small and unfused bones (assigned as pup remains) of Otaria flavescens (O. flavescens) in the Patagonian archaeological record has been frequently used as an indicator of summer occupation accomplished by ecological analogues. For this reason, we carried out a postcranial skeletons osteometric study of modern pups of South American sea lions (O. flavescens) with known sex and aged 0?12months, allowing a more precise age estimation of archaeological samples. Then, we revisited the evidence for seasonality in the Cabo Virgenes 6 site at Cabo Virgenes locality, one of the archaeological localities in Patagonia where the seasonality has been determined only on the basis of the presence of unfused pup bones. Our metric data study on amodern sample shows that the regression analyses generated a complementary and dependable age estimation model for unfused appendicular bones of O. flavescens between 0 and 12 months old that can be applied to samples recovered from archaeological sites. These osteometric studies of postcranial elements allow us to adjust the age estimation of the animal?s death and re-examine seasonality of theCabo Virgenes 6 site (SantaCruz, Argentina).Copyright©2013 John Wiley &amp; Sons, Ltd","palabras_clave":"SOUTH AMERICAN SEA LIONS|OTARIIDS PUPS|POSTCRANIAL OSTEOMETRY|SEASONALITY INDICATORS|HUNTER-GATHERER|COASTAL ARCAEOLOGICAL SITES|PATAGONIA|HOLOCENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1010,"idioma_id":4,"titulo":"Transference of Wheat Expressed Sequence Tag-Simple Sequence Repeats to Paspalum Species and Cross-Species Amplification of Paspalum notatum Simple Sequence Repeats: Potential Use in Phylogenetic Analysis and Mapping","resumen":"The genus Paspalum L. includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat (Triticum aestivum L.) expressed sequence tag (EST)?simple sequence repeat (SSR) (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum Flüggé genomic SSRs (PnSSRs) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty-two accessions including 10 species were used. Moreover, 65 F1 hybrids of P. notatum were used for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSR data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both wEST-SSRs and PnSSRs mapped scattered in the genome. One hundred two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219, and PN03 F2 generated markers linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23 to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.","palabras_clave":"Paspalum|EST-SSR|mapping |phylogeny"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1011,"idioma_id":4,"titulo":"A-type zeolite containing Ag+\/Zn2+ as inorganic antifungal for waterborne coating formulations","resumen":"The biocide cations Ag+ and Zn2+ were hosted in the cavities of an ordered aluminosiliceous framework.Starting from sodium A-type zeolite (NaA), LTA containing Ag+ (AgA), Zn2+ (ZnA) and Ag+\/Zn (AgZnA) at  different cation exchanged levels was obtained and its antifungal properties were evaluated. To determine the minimum inhibitory concentration (MIC) of the exchanged zeolites against Aspergillus niger,  [Ag+] and [Zn2+] values ranging from 50 < [Ag+] < 1000 mg L&#8722;1 to 650 < [Zn2+] < 2000 mg L&#8722;1, respectively,were used for NaA, and for AgZnA: 30 < Ag+ < 250 mg L&#8722;1. The zeolite sample having [Ag+] = 100 mg L&#8722;1,  [Zn2+] = 90 mg L&#8722;1 produces a growth inhibition comparable to that achieved with 230 mg L&#8722;1 of Ag+1 (MIC value obtained for the single cation). The antifungal activity of these products after incorporation  in waterborne coating formulations was also determined. Results indicate that Ag+ and Zn2+ supported on A-type zeolite could be a beneficial tool for the development of waterborne coatings with a longer protection against microbiological attack when compared to traditional organic biocides.","palabras_clave":"A- type zeolite|Inorganic biocides|Waterborne coating|Aspergillus niger"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1012,"idioma_id":4,"titulo":"Interplay between postcranial morphology and locomotor types in Neotropical sigmodontine rodents","resumen":"Sigmodontine rats are one of the most diverse components of the Neotropical mammal fauna. They exhibit a wide ecological diversity and a variety of locomotor types that allow them to occupy different environments.To explore the relationship between morphology and locomotor types, we analyzed traits of the postcranial osteology (axial and appendicular skeletons) of 329 specimens belonging to 51 species and 29 genera of sigmodontines exhibiting different locomotor types. In this work, postcranial skeletal characters of these rats are considered in an ecomorphological study for the first time. Statistical analyses showed that of the 34 osteological characters considered, 15 were related to the locomotor types studied, except for ambulatory. However, character mapping showed that climbing and jumping sigmodontines are the only taxa exhibiting clear adaptations in their postcranial osteology, which are highly consistent with the tendencies described in many other mammal taxa. Climbing, digging and swimming rats presented statistically differences in traits associated with their vertebral column and limbs, whereas jumping rats showed modifications associated with all the skeletal regions. Our data suggest that sigmodontine rats retain an all-purpose morphology that allows them to use a variety of habitats. This versatility is particularly important when considering the lack of specialization of sigmodontines for a specific locomotor mode. Another possible interpretation is that our dataset probably did not consider relevant information about these groups and should be increased with other types of characters (e.g. characters from the external morphology, myology, etc.).","palabras_clave":"ECOMORPHOLOGICAL ANALYSIS  |POSTCRANIUM|LOCOMOTION|SIGMODONTINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1013,"idioma_id":4,"titulo":"Self-Assembled Redox Polyelectrolyte-Surfactant Complexes: Nanostructure and Electron Transfer Characteristics of Supramolecular Films with Built-In Electroactive Chemical Functions","resumen":"The mesostructural and electrochemical characterization of a redox-active polyelectrolyte-surfactant complex formed by polyallylamine tagged with an osmium complex and dodecylsulfate is presented. X-ray reflectivity (XRR), grazing-incidence small-angle X-ray scattering (GISAXS), X-ray photoelectron spectroscopy (XPS), contact angle goniometry (CA) and cyclic voltammetry (CV), including the numerical simulation of the voltammetric response, were employed to analyze the structure, stability and the electrochemical response of these supramolecular films. In contrast to redox-active polyelectrolyte multilayers (PEMs), the self-assembled system presented here shows a mesoscopic order  yielding a film of layered structure very stable to an aqueous environment where the hydrophilic moieties (amino and sulfate groups) are not exposed to the solution since a contact angle of 95º is observed upon exposure to water. However, the film shows a self-exchange electron transfer mechanism with an apparent diffusion coefficient of 2x10-9 cm2 s-1 for a film of 300 nm of thickness. This behavior shows that the film exposed to an aqueous solution undergoes a fast electron transfer process to\/from the electrode surface and ions to\/from the electrolyte solution.","palabras_clave":"Supramolecular Assembly|Polyelectrolyte-Surfactant Complexes|Self-Organized Materials|Electroactive Materials"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1016,"idioma_id":4,"titulo":"SMALLANTHUS MACROSCYPHUS: A NEW SOURCE OF ANTIDIABETIC COMPOUNDS","resumen":"The aim of the present study was to analyze the in vivo hypoglycaemic effects of both decoction of Smallanthus macroscyphus leaves and pure crystalline polymatin A isolated from its leaves. Phytochemical analysis of the leaf decoction showed that its major constituents were caffeic, chlorogenic and three dicaffeoilquinic acids, together with thesesquiterpene lactone polymatin A. Oral glucose tolerance test in normal rats was performed to evaluate the hypoglycemic activity and to choose the minimum effective dose of the decoction and polymatin A. They have effective hypoglycemic activity at the minimum dose of 140 mg dry extract and 14 mg crystalline powder\/kg body weight, respectively, and were selected for the following experiments. Oral administration of a single-dose of decoction produced a moderate lowering effect in fasting glycemia of normal rats, whereas polymatin A had no significant effect. We also assessed the effect of a single-dose on post-prandial blood glucose, resulting in an inhibition of the hyperglycemic peak after sucrose overload. Daily administration of decoction or polymatin A for 4 weeks produced an effective glycemic control in diabetic animals, with a decrease in urinary glucose excretion and a significant reduction in the HbA1c levels. Although there were no significant increases in plasma insulin levels, both treatments improved the fasting blood glucose\/insulin ratio. In vivo acute toxicity studies were performed in adult Wistar rats. There were no deaths or signs of toxicity observed after oral administration of decoction or polymatin A at any dose level up to the highest dose tested (14.0 g \/kg and 2.8 g \/kg, respectively).","palabras_clave":"SMALLANTHUS MACROSCYPHUS LEAVES|POLYMATIN A|HYPOGLYCEMIC ACTIVITY|ANTIDIABETIC PRINCIPLES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1018,"idioma_id":4,"titulo":"The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina","resumen":"American tegumentary leishmaniasis (ATL) is a group of zoonotic diseases caused by kinetoplastid flagellates of the genus Leishmania. A total of 66 patients diagnosed as positive ATL cases from northwest Argentina were included in this study. Leishmania stocks were isolated in vitro and analyzed over promastigote cultures sown on FTA through nested PCR and sequence of cytochrome b (cyt b). The molecular analysis resulted in the incrimination of L. (Viannia) braziliensis as the predominant species in the studied area, identifying two genotypes of L. (V.) braziliensis, 24 cases of Ab-1 cyt b and 41 cases of Ab-2 cyt b. One L. (V.) guyanensis strain was obtained from a traveler from the Brazilian Amazon. The prevalence of different genotypes was in agreement with previous studies, suggesting the necessity for new systems to study the genetic diversity in more detail. Most of the cases typified in this study were registered in the area of Zenta Valley (Orán, Hipólito Yrigoyen, and Pichanal cities), pointing a link between genotype and geographical origin of the sample. Sex and age distribution of the patients indicate that the transmission was predominantly associated with rural areas or rural activities, although the results might not exclude the possibility of peri-urban transmission. This work represents, so far, the largest isolation and molecular characterization of ATL cases in Argentina.","palabras_clave":"LEISHMANIA|ARGENTINA|CYTOCHROME B|TIPIFICACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1019,"idioma_id":4,"titulo":"Microcystis aeruginosa strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina","resumen":"Objective: To show the toxicological and phylogenetic characterization of a native Microcystis  aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine.   Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive\/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC\/ESI-HRMS microcystins analysis. The number of cell\/mL and [D-Leu1] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation.  Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa   with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS\/Orbitrapas as [D-Leu1  ] microcystin-LR ([M+H]+=1 037.8 m\/z).   Conclusions: [D-Leu1] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.","palabras_clave":"Cyanobacteria|Microcystis aeruginosa|[D-Leu1 ] microcystin-LR [M+H]+|Harmful algal blooms"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1020,"idioma_id":4,"titulo":"First finding of genetic and antigenic diversity in 1b-bvdv isolates from argentina","resumen":"nfection with Bovine Viral Diarrhea Viruses (BVDV) in cattle results in a wide range of clinical manifestations, ranging from mild respiratory disease to fetal death and mucosal disease, depending on the virulence of the virus and the immune and reproductive status of the host. In this study 30 Argentinean BVDV isolates were characterized by phylogenetic analysis. The isolates were genotyped based on comparison of the 5' untranslated region (5' UTR) and the E2 gene. In both phylogenetic trees, 76% of the viruses were assigned to BVDV 1b, whereas BVDV 1a, 2a and 2b were also found. Eight of the BVDV 1b isolates were further characterized by cross-neutralization tests using guinea pig antisera and sera from bovines vaccinated with two different commercial vaccines. The results demonstrated the presence of a marked antigenic diversity among Argentinean BVDV isolates and suggest the need to incorporate BVDV 1b isolates in diagnostic strategies.","palabras_clave":"Virus|DVB"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1021,"idioma_id":4,"titulo":"The influence of soil properties on denitrifying bacterial communities and denitrification potential in no-till production farms under contrasting management in the Argentinean Pampas","resumen":"The aim of this work was to investigate the response of the structure, abundance and potential activity of denitrifiers to contrasting agricultural management in no-till production fields, across a regional scale within the highly productive Argentine northern Pampas. Treatment categories were grouped according to the sustainability of the soil management, in terms of crop rotation, fertilization, agrochemicals use and pest control, as good no-till agricultural practices (GAP) and poor no-till agricultural practices (PAP). Non-cultivated soils in each geographic location were also evaluated as reference groups. Mixed\tmodels,\twith sites\ttreated\tas random factors,\tindicated\tthat\tthe\tpotential denitrification activity and the size of the nirS community differed significantly between non-cultivated and PAP soils. Soil properties were separated into dynamic and inherent according to their variance components. The former had the largest part of their variances explained by agricultural management, while the latter were more affected by edaphic-climatic differences between sites. Both inherent and dynamic properties could explain the changes in potential denitrification activity, whereas changes in the abundance of denitrifiers were only related to inherent soil properties. Results from principal components analysis suggested site-specific response of most dynamic soil properties. Among the latter, only aggregate stability indices were strongly associated with potential\tdenitrification activity after removing the geographical effect.","palabras_clave":"No tillage|Denitrification|Structural stability indices|Sustainable agriculture"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1022,"idioma_id":4,"titulo":"Dissolution properties, solid-state transformation and polymorphic crystallization: progesterone case study","resumen":"Progesterone is a natural steroid hormone and a poor soluble drug which presents two  polymorphs (forms 1 and 2). Different methods to obtain form 2 were tested and a complete  solid-state characterization of both polymorphs (forms 1 and 2) was conducted. X-ray powder  diffraction, hot stage microscopy, Fourier transform infrared, dispersive Raman, 13C solid-state  nuclear magnetic resonance spectroscopy, thermal analysis, scanning electron microscopy  techniques and intrinsic dissolution rates (IDR) were applied to investigate physical?chemical  and dissolution properties of these two polymorphs. Form 2 was obtained from diluted  solutions and from melting after cooling at room temperature. Form 1 was obtained from  concentrated solutions and, a mixture of both polymorphs was crystallized from intermediate  solutions. The crystal habit was not a distinctive characteristic of each polymorph. The effect of  mechanical stress was evaluated in the metastable polymorph (form 2). We observed that  grinding form 2 produced seeds of form 1 that induced the transformation of form 2 into form  1 at high temperature. The polymorphic quantification from XRD patterns of ground samples  were carried out by the Rietveld method. After grinding and at room temperature conditions  (25 C), it was observed the transformation of 17% of form 2 into form 1 in 10 days.","palabras_clave":"Intrinsic dissolution rate|phase transformation|polymorphism|progesterone"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1024,"idioma_id":4,"titulo":"Fabrication of ferrogels using different magnetic nanoparticles and their performance on protein adsorption","resumen":"Magnetic biomaterials were prepared using magnetite and chitosan-coated magnetite nanoparticles (CSNPs) dispersed in  poly(vinyl alcohol) gels. Two different methods were developed to obtain ferrogels: in situ co-precipitation of magnetite  (Ferro-IS) and by adding previously synthesized CSNPs to the neat matrix (Ferro-CSNPs). In both cases, the crosslinking was  carried out by freezing thawing. The as-prepared materials as well as precursor CSNPs were characterized by Fourier  transform infrared spectroscopy, electronic microscopy (scanning and transmission), X-ray diffraction, zeta potential, dynamic  light scattering, thermogravimetric analysis, differential scanning calorimetry and magnetic properties. The performance of  these gels as protein adsorbents was evaluated. Batch adsorption experiments were carried out using bovine serum albumin  (BSA) as amodel. Substantially different adsorption behaviour was found using Ferro-IS and Ferro-CSNPs. This was assigned to  dissimilar bonding mechanisms of BSA to the ferrogelmatrix. Hence, biomaterials potentially useful in drug delivery as well as  in protein purification fieldsmay be prepared by a relatively simple, non-toxic and low cost method.","palabras_clave":"biomaterials|iron oxides|magnetic materials|ferrogel"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1025,"idioma_id":4,"titulo":"Density functional study of acrolein adsorption on Pt (111)","resumen":"The electronic structure and bonding of acrolein (propenal)  adsorption on Pt (111) were studied by density functional calculations (DFT).  We optimized different adsorption configurations on the surface using the VASP  code. The energetic study indicates that acrolein trans isomers are less stable  on the Pt surface than cis configurations, which have a similar stability in  all cases. We found that the inclusion of Van der Waals (vdW) corrections does  not change the obtained adsorption geometries or preferential sites relative  energies. We also analyzed the evolution of the chemical bonding changes in the  adsorbate and the metal surface by crystal orbital overlap population (COOP)  and overlap population (OP) analysis of selected bonds. Except for the &#951;1 configuration, the adsorption of acrolein on the surface is mainly due  to the formation of C-Pt bonds. We also found that the acrolein C pz orbital,  perpendicular to the surface, play an important role in the adsorption, as well  as Pt pz and dz 2 orbitals, whose lobes are well oriented to overlap with the  adsorbate orbitals.  ","palabras_clave":"Acrolein|Pt (111)|Adsorption|DFT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1027,"idioma_id":4,"titulo":"Enhancing vibration measurements by Mössbauer effect","resumen":"The measurement of the Mössbauer effect in a system excited with a periodic perturbation can provide information about it. For that purpose, the Mössbauer absorption of a source-absorber set which hyperfine parameters are well known, is measured at a constant relative velocity (i.e. at a defined spectral energy). The resulting Mössbauer absorption periodic signal provides information of the sample ac perturbation response. This approach has been used time ago to measure small tympanic vibrations (mechanical perturbations). In this work we present an extension of the vibration experiments, by measuring them at various absorber-source relative velocities within a constant-velocity strategy. As a demonstration test, the frequency response of a piezoelectric diaphragm in the 100 Hz?5 kHz range is obtained with a custom electronic counter. The experiments are performed using a 57Co(Rh) source and a 25-um-thick stainless-steel absorber fixed to a piezoelectric diaphragm. Phase shifts and amplitude vibrations with velocities in the range from 1.5 um\/s to 20 mm\/s are well characterized, extending the linearity limit well beyond the earlier suggested one of 1 mm\/s.","palabras_clave":"Vibrations|Masuremenst|relative velocity|Mössbauer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1028,"idioma_id":4,"titulo":"The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer","resumen":"Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNã, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.","palabras_clave":"transcriptome |low-abundance |biomarkers|advanced gastric cancer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1031,"idioma_id":4,"titulo":"Reassessment of MLST schemes for Leptospira spp. typing worldwide","resumen":"Leptospirosis is a neglected zoonosis of global importance. Several multilocus sequence typing (MLST) methods have been developed for Leptospira spp., the causative agent of leptospirosis. In this study we reassessed the most commonly used MLST schemes in a set of worldwide isolates, in order to select the loci that achieve the maximum power of discrimination for typing Leptospira spp. Global eBURST algorithm was used to detect clonal complexes among STs and phylogenetic relationships among concatenated and individual sequences were inferred through maximum likelihood (ML) analysis. The evaluation of 12 loci combined to type a subset of strains rendered 57 different STs. Seven of these loci were selected into a final scheme upon studying the number of alleles and polymorphisms, the typing efficiency, the discriminatory power and the ratio dN\/dS per nucleotide site for each locus. This new 7-locus scheme was applied to a wider collection of worldwide strains. The ML tree constructed from concatenated sequences of the 7 loci identified 6 major clusters corresponding to 6 Leptospira species. Global eBURST established 8 CCs, which showed that genotypes were clearly related by geographic origin and host. ST52 and ST47, represented mostly by Argentinian isolates, grouped the higher number of isolates. These isolates were serotyped as serogroups Pomona and Icterohaemorrhagiae, showing a unidirectional correlation in which the isolates with the same ST belong to the same serogroup. In summary, this scheme combines the best loci from the most widely used MLST schemes for Leptospira spp. and supports worldwide strains classification. The Argentinian isolates exhibited congruence between allelic profile and serogroup, providing an alternative to serological methods.","palabras_clave":"LEPTOSPIRA SP.|MULTILOCUS SEQUENCE TYPING|REASSESSMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1032,"idioma_id":5,"titulo":"Memoria Autobiográfica, Sentidos y Fenomenología: Recuerdos de Tipo Traumático en Ex-combatientes y Veteranos de la Guerra de Malvinas","resumen":"Desde la psicología cognitiva, la memoria autobiográfica se encuentra implicada en tareas de consolidación, evocación y olvido de información personal y episodios de vida. Los recuerdos autobiográficos conformados a partir de vivencias traumáticas, tales como un combate bélico, asumirían propiedades distintivas. El objetivo del trabajo fue analizar las características principales presentes en auto-reportes escritos de ex-combatientes y veteranos hombres de la Guerra de Malvinas respecto de vivencias traumáticas ocurridas durante el combate. El estudio, de tipo exploratorio cualitativo, adscribió al paradigma interpretativo bajo los principios de la teoría fundamentada en los hechos, utilizando un muestreo teórico intencionado. Fueron analizados de modo descriptivo, axial y selectivo 141 formularios. Se identificaron 3 grupos de categorías emergentes del discurso: componentes fenomenológicos, muerte\/riesgo de muerte y perdurabilidad. El entrecruzamiento de los aspectos cognitivos y temporales pone en evidencia, a modo de categoría central, el sentido de vigencia que para ex-combatientes y veteranos asumen las vivencias reportadas. Se sugiere la implementación de intervenciones de carácter psicosocial orientadas al reconocimiento de los recuerdos traumáticos en pos de su integración al relato colectivo de Malvinas.","palabras_clave":"MEMORIA|RECUERDOS AUTOBIGRAFICOS|MALVINAS|RE-EXPERIMENTACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1033,"idioma_id":4,"titulo":"Diversity of Potassium Channels in Human Umbilical Artery Smooth Muscle Cells: A Review of Their Roles in Human Umbilical Artery Contraction","resumen":"Through their control of cell membrane potential, potassium (K+) channels are among the best known regulators of vascular tone. This article discusses the expression and function of K+ channels in human umbilical artery smooth muscle cells (HUASMCs). We review the bibliographic reports and also present single-channel data recorded in freshly isolated cells. Electrophysiological properties of big conductance, voltage- and Ca2+-sensitive K+ channel and voltage-dependent K+ channels are clearly established in this vessel, where they are involved in contractile state regulation. Their role in the maintenance of membrane potential is an important control mechanism in the determination of the vessel diameter. Additionally, small conductance Ca2+-sensitive K+ channels, 2-pore domains K+ channels and inward rectifier K+ channels also appear to be present in HUASMCs, while intermediate conductance Ca2+-sensitive K+ channels and ATP-sensitive K+ channels could not be identified. In both cases, additional investigation is necessary to reach conclusive evidence of their expression and\/or functional role in HUASMCs. Finally, we discuss the role of K+ channels in pregnancy-related pathologies like gestational diabetes and preeclampsia.","palabras_clave":"human umbilical artery|K+ channels|patch-clamp"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1035,"idioma_id":4,"titulo":"Aerosol optical properties in Central Argentina","resumen":"This work presents the analysis of the long-term observations of aerosol optical properties in the central region of Argentina. Monitoring of aerosol parameters was carried out at the Cordoba-CETT AERONET site (31º 31? S, 64º 27? W, 730 m.a.s.l.) from November 1999 until December 2010. Long-term measurements of aerosol optical depth, Ångström exponent, fine mode fraction and single scattering albedo were analyzed and compiled to describe the climatology of the optical properties of the aerosols of the region. The knowledge of the optical properties of aerosols and their spatial distribution is required to evaluate aerosol effects on the climate system. This information provides an opportunity for understanding how aerosols might influence the regional radiation budget. Results show that aerosol optical depth at 340 nm is characterized by low values from February to April (monthly average of 0.15 ±0.05), very low values from May to June (monthly average of 0.08 ±0.03) and a sustained increase from July to September (monthly average of 0.20 ±0.09) reaching a value of 0.26. From this dataset, no long-term trends are observable. Results of the inter-annual variations of the Ångström exponent between 440 and 870 nm reflect an important difference in the year 2004 compared to the other 11 years of the study. A possible explanation of this fact is elaborated with the help of back trajectory analysis. Finally, three episodes are described and analyzed, as they produced important increases of the daily aerosol optical depth value. We explained these episodes with a combination of air mass trajectory analysis, meteorology and the MODIS fire counts product.","palabras_clave":"Cordoba region|AERONET|AEROSOL OPTICAL PROPERTIES|BACK TRAJECTORIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1036,"idioma_id":4,"titulo":"Triacylglycerol content, productivity and fatty acid profile in Scenedesmus acutus PVUW12","resumen":"A detailed lipid characterization of Scenedesmus  acutus PVUW12, with emphasis on the evaluation of triacyl-  glycerols (TAGs) as a biodiesel feedstock, is presented.When  algal cells were grown in nitrogen-free medium (N stress), a  lipid increase was detected that was mainly due to TAG  accumulation. In situ fluorescence measurements allowed  the kinetics and extent of neutral lipid accumulation to be  followed. Under N stress, the productivity of total lipids and  TAGs increased significantly (80.99 and 63.74 mg L&#8722;1  day&#8722;1  ,  respectively) compared with controls (29.51 and  16.23 mg L&#8722;1  day&#8722;1  , respectively). Monounsaturated fatty  acids were the major fraction and increased further","palabras_clave":"MICROALGAS|BIODIESEL|GRASAS|LIPIDOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1037,"idioma_id":4,"titulo":"Optimization and in vivo toxicity evaluation of G4.5 PAMAM Dendrimer-Risperidone complexes","resumen":"Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (.90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence onsolvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50:50 v\/v solution pH 3. In addition, to explore the possible effects of this complex,in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiologicalchanges were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.","palabras_clave":"G4.5 PAMAM dendrimers|nanocarriers |Zebrafish|in vivo studies"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1038,"idioma_id":4,"titulo":"Nitric oxide sets off an antioxidant response in adrenal cells: involvement of sGC and Nrf2 in HO- 1 induction","resumen":"Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in  different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production  as well. As nitric oxide (NO) has been also regarded as an autocrine\/paracrine modulator of adrenal  steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism  involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA\/NO) on HO-1  induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional  level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by  DETA\/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress  as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the  nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated  by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of  the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific  inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2.   2013 Elsevier Inc. All rights reserved.","palabras_clave":"Nitric Oxide|HO1|antioxidant response|adrenal cells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1039,"idioma_id":4,"titulo":"DFT  studyofbenzeneandCOco-adsorptiononPtCo(111)","resumen":"Co-adsorption of benzene and CO on PtCo(1 1 1) surface at low coverage is studied using density functional theory calculations. We investigated the PtCo FCT alloy surface with a uniform distribution. The most favorable site for CO is top on a Pt atom whereas for benzene is an HCP hollow site (formed by 2 Pt atoms and 1 Co atom). The co-adsorption energy is &#8722;1.62 eV. The calculations indicate a CO molecule with a &#8764;4&#9702; tilt angle with the normal to the surface. The most important bond is Pt CCO, as revealed by overlap population analysis. A very small CO?benzene interaction is also detected. The vibrational frequencies of adsorbed benzene and CO were also computed.","palabras_clave":"benzene"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1040,"idioma_id":4,"titulo":"Cluster B personality symptoms in persons at genetic risk for schizophrenia are associated with social competence and activation of the right temporo-parietal junction during emotion processing","resumen":"Personality disorders are common in nonpsychotic siblings of patients   with schizophrenia, and some personality traits in this group may be   associated with an increased risk for full-blown psychosis. We sought to   establish if faulty right-hemisphere activation induced by social   cognitive tasks, as previously described in patients with schizophrenia,   is associated with specific personality symptoms in their unaffected   siblings. We observed that cluster B personality symptoms in this group   were inversely related to activation in the right temporo parietal   junction (rTPJ, a structure critical in social cognitive processing) in   response to a basic emotion processing task and also to social   competence, whereas in contrast to our initial hypothesis, cluster A   traits were not associated with right hemisphere activation during   emotion processing or with social competence. These findings suggest the   existence of clinical traits in at-risk individuals which share a   common neurobiological substrate with schizophrenia, in regards to   social performance.","palabras_clave":"fMRI|Schizophrenia|Siblings|Cluster B"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1041,"idioma_id":4,"titulo":"A Simple and Efficient Procedure for the Synthesis of Ferrogels based on Physically Crosslinked PVA","resumen":"Ferrogels with well dispersed single-domain magnetic NPs were obtained by infusion of   iron salts in physically crosslinked PVA hydrogels followed by coprecipitation.   Freezing thawing (F-T) was used as a cryogenic technique to form mechanically strong   and highly swellable hydrogels. Networked structure of the final material was used as a   constrained environment useful for the precipitation of iron oxide NPs and formation of   the magnetic gel. A homogeneous, single domain ensemble of more than 15 wt% of   iron oxide NPs (in only one cycle of absorption) could be obtained through this easy   technique. Moreover, capacity of these magnetic ferrogels to absorb high amounts of   ethanol\/water solutions allows impregnation of these materials with ibuprofen and   subsequent release of the drug at physiological pH. Biocompatibility of the components   and the use of a non-toxic PVA crosslinking strategy (F-T) make these materials   promising for drug delivery applications.","palabras_clave":"Ferrogel|Magnetita|PVA|Freezing thawing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1042,"idioma_id":4,"titulo":"HYDROGEOCHEMISTRY AND ISOTOPE TECHNIQUES TO DETERMINE WATER INTERACTIONS IN GROUNDWATER DEPENDENT SHALLOW LAKES, WET PAMPA PLAIN, ARGENTINA","resumen":"This paper gives an account of the implementation of hydrochemical and isotopic techniques to identify and explain the processes that govern solute exchange in two groundwater-dependent shallow lakes in the Southeastern Pampa Plain of Argentina. Water samples (lakes, streams, spring water and groundwater) for hydrochemical and stable isotopic determination were collected and the main physical?chemical parameters were measured. The combination of stable isotope data with hydrogeochemical techniques was used for the identi&#64257;cation of sources and  preferential recharge areas to these aquatic ecosystems which allowed the explanation of the lake water origin. The hydrochemical processes which explain Los Padres Lake water chemistry are evaporation from groundwater, CO2 input, calcite dissolution, Na? release by Ca2? and Mg2? exchange, and sulfate reduction. The model that best aligns with La Brava Lake hydrochemical constraints includes: mixing, CO2 and calcite dissolution, cationic exchange with Na? release and Mg2? adsorption, and to a lesser extent, Ca\/Na exchange. This model suggests that the fractured aquifer   contribution to this water body is greater than 50 %. An isotopic-speci&#64257;c &#64257;ngerprint for each lake was identi&#64257;ed, &#64257;nding a higher evaporation rate for La Brava Lake compared to Los Padres Lake. Isotopic data demonstrate the importance of these shallow lakes as recharge areas to the regional aquifer, becoming areas of high groundwater vulnerability. The Tandilia Range System, considered in many hydrogeological studies as the  impermeable bedrock of the Pampean aquifer, acts as a &#64257;ssured aquifer in this area, contributing to low salinity waters and with a &#64257;ngerprint similar to groundwater isotopic composition.","palabras_clave":"ENVIRONMENTAL ISOTOPES|HYDROGEOCHEMISTRY|SOLUTE EXCHANGE|GROUNDWATER\/SURFACE WATER INTERACTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1043,"idioma_id":4,"titulo":"Automatic classification of legumes using leaf vein image features","resumen":"In this paper, a procedure for segmenting and classifying scanned legume leaves based only on the analysis of their veins is proposed (leaf shape, size, texture and color are discarded). Three legume species are studied, namely soybean, red and white beans. The leaf images are acquired using a standard scanner. The segmentation is performed using the Unconstrained Hit-or-Miss Transform and adaptive thresholding. Several morphological features are computed on the segmented venation, and classified using four alternative classifiers, namely Support Vector Machines (linear and Gaussian kernels), Penalized Discriminant Analysis and Random Forests. The performance is compared to the one obtained with cleared leaves images, which require a more expensive, time consuming and delicate procedure of acquisition. The results are encouraging, showing that the proposed approach is an effective and more economic alternative solution which outperforms the manual expert?s recognition.","palabras_clave":"LEAF VEIN FEATURES|LEAF VEIN IMAGES|LEGUME CLASSIFICATION|LEAF VEIN ANALYSIS|UNCONSTRAINED HIT-OR-MISS TRANSFORM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1044,"idioma_id":5,"titulo":"Adultos mayores y monitoreo de derechos. Alcances y limitaciones de las fuentes de información en Argentina y Uruguay","resumen":"El proceso de envejecimiento en los países de la región ha configurado una demanda de investigación que representa nuevos desafíos teóricos y metodológicos; entre ellos, la posibilidad de realizar un seguimiento basado en el enfoque de los derechos humanos. En este marco, se plantea cuáles son las posibilidades de avanzar en el conocimiento de la problemática de los adultos mayores con base en la información disponible en Argentina y Uruguay. El trabajo pretende explorar las fuentes de datos producidas en ambos países durante la última década al tomar como referencia la propuesta de indicadores de CELADE (2006), con mirada desde los derechos humanos.  Los resultados indican que existe la posibilidad de construir el sistema de indicadores sugerido, aunque con ciertas limitaciones en lo que hace al Enfoque Basado en los Derechos Humanos (EBDH). También evidencian la necesidad de generar nuevas fuentes de datos, que permitan captar información orientada al seguimiento de los derechos humanos de los adultos mayores, en todos sus ámbitos (y mejorar, particularmente, la recolección de datos sobre salud y sobre entornos favorables).","palabras_clave":"FUENTES DE DATOS|ENVEJECIMIENTO|DERECHOS HUMANOS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1045,"idioma_id":4,"titulo":"Inhibition of nitric oxide is a good therapeutic target for bladder tumors that express iNOS","resumen":"Bladder cancer is the second cause of death for urological tumors in man. When the tumor is nonmuscleinvasive, transurethral resection is curative. On the other hand, radical cystectomy is the treatment chosenfor patients with invasive tumors, but still under treatment, these patients have high risk of dying, bythe development of metastatic disease within 5 years. It is therefore important to identify a new therapeutictarget to avoid tumor recurrences and tumor progression. Nitric oxide (NO) is an important biologicalmessenger known to influence several types of cancers. In bladder cancer, production of NOand expression and activity of inducible NO synthase was associated to recurrence and progression.The objective of this work was to analyze if inhibition of nitric oxide production could be considered atherapeutic target for bladder tumors expressing iNOS. Using a bladder cancer murine model with differentinvasiveness grade we have demonstrated that NO inhibition was able to inhibit growth of bladdertumors expressing iNOS. Furthermore, invasive properties of MB49-I orthotopic growth was inhibitedusing NO inhibitors. This paper also shows that levels of NO in urine can be correlated with tumor size.In conclusion, inhibition of NO could be considered as a therapeutic target that prevents tumor growthand progression. Also, urine NO levels may be useful for measuring tumor growth.","palabras_clave":"Nitric oxide |Bladder cancer |iNOS |Orthotopic murine model"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1046,"idioma_id":4,"titulo":"Minor Anomalies: Can They Predict Specific Major Defects? A Study Based on 23 Major and 14 Minor Anomalies in Over 25,000 Newborns with Birth Defects.","resumen":"Background: Minor anomalies (mAs) are morphologic features with little clinical relevance that have been mentioned as possible predictors of major defects (MDs).  Objectives: To identify preferential associations between selected MDs and mAs and to establish if mAs can serve as predictors for specific MDs.  Study design: Information of infants with birth defects was obtained from the ECLAMC (Latin American Collaborative Study of Congenital Malformations)database. The sample consisted of 27,247 live- and stillborn infants with multiple malformations that included at least one of the selected MDs or mAs. Odds ratios and predictive values were calculated for significant associations, and concurrence  rates in first degree relatives.  Results: Thirty-three significant minor-major associations were identified. Single umbilical artery (SUA) and preauricular tags were the most frequent mAs; the former was associated with ten MDs, the latter only with microtia. The highest positive predictive value was shown by SUA for anal atresia. Infants with preauricular tags had significantly more relatives with microtia than expected.  Conclusions: No new relevant associations between MDs and mAs were  identified and few mAs seem to serve as predictors for specific MDs in the same infant. However, preauricular tags can predict the occurrence of microtia in other family members","palabras_clave":"epidemiology|minor anomalies|birth defects|predictors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1048,"idioma_id":4,"titulo":"Female mate choice by chemical signals in a semi-terrestrial crab","resumen":"Information about the roles of both sexes in pair formation is required to better understand the mechanisms involved in sexual selection. Mate choice could depend on the courtship behavior, involving chemical, tactile and visual signals. We determined if Neohelice granulata mate choice is based on female or male choice,considering visual and chemical with contact and without contact signals between partners and different  categories of individuals: receptive and unreceptive females; and large, small, mated or unmated males. Experiments showed that mate selection was based on receptive female´s choice using chemical signals,but not visual ones. Since copulation occurs during high and low tides, water-borne chemical signals would be preferentially used during high tide, while contact ones during low tide. Females preferred large and unmated males, while males did not seem to recognize receptive females using chemical neither visual signals. Females were capable of detecting the presence of the chemical signals released by large and unmated males, but not its amount. It is proposed that small and mated males are probably releasing different types of chemical signals, not attractive to females, or that they are not emitting any signal.","palabras_clave":"mate choice|chemical signals|visual signals|varunidae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1050,"idioma_id":5,"titulo":"Trabajo infantil en la ciudad de Córdoba, Argentina, en el primer tercio del siglo XIX","resumen":"Durante el primer tercio del siglo XIX la ciudad de Córdoba experimentó la transición del régimen borbónico a los primeros intentos de un gobierno republicano. El objetivo de esta investigación fue examinar la situación de la infancia cordobesa de entonces en el sentido de en qué ocupaban los niños su tiempo, específicamente en el aspecto laboral y a qué pudieron deberse las variaciones temporales respecto de aquél. Para ello se analizaron los censos de 1813 y 1832 en relación con la población infantil y la ocupación por franja etaria, grupo etnosocial y sexo. Se observó una tasa de empleo elevada, sobre todo en edades más avanzadas y entre no españoles, y mayor peso relativo de oficios en varones y servicio doméstico en mujeres; entre censos, el trabajo fue más conspicuo en 1813, cuando la profesión estudiante tuvo menor incidencia. Se notaron pervivencias de lo aquí encontrado en épocas posteriores.","palabras_clave":"HISTORIA DE LA POBLACIÓN|NIÑEZ|TRABAJO INFANTIL|GRUPO ETNO-SOCIAL|CIUDAD DE CÓRDOBA |CENSO DE POBLACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1051,"idioma_id":4,"titulo":"Modeling total volatile basic nitrogen production as a dose function in gamma irradiated refrigerated squid rings","resumen":"The behavior of a chemical spoilage index for marine products (total volatile basic nitrogen e TVBN) was modeled as a function of the irradiation dose in Illex argentinus rings stored in refrigeration. The effect of gamma irradiation at 0, 1.8, 3.3 and 5.8 kGy on TVBN was analyzed in vacuum-packed squid rings during storage at 4   1  C. The modified Gompertz model satisfactorily described TVBN behavior for each irradiation dose (R2 > 0.980; RMSE < 5.7). Gompertz model parameters (m, A, L) were modeled with dose-dependent second order polynomials, for doses ranging between 0 and 5.8 kGy, in order to develop a complete model that is useful for predicting TVBN as a function of the dose in squid rings. Model validation was carried out using independent data of TVBN in squid rings irradiated at 4.8 kGy. The developed model can be used to predict TVBN production in gamma irradiated squid meat.","palabras_clave":"IONISING RADIATION|MATHEMATICAL MODELING|VOLATILE BASIC NITROGEN|SQUID"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1052,"idioma_id":4,"titulo":"Effects of pH and sugar concentration in Zygosaccharomyces rouxii growth and time for spoilage in concentrated grape juice at isothermal and non-isothermal conditions","resumen":"The effect of pH (1.7e3.2) and sugar concentration (64e68 Brix) on the growth of Zygosaccharomyces rouxii MC9 using response surface methodology was studied. Experiments were carried out in concentrated grape juice inoculated with Z. rouxii at isothermal conditions (23 C) for 60 days. pH was the variable with the highest effect on growth parameters (potential maximum growth rate and lag phase duration), although the effect of sugar concentration were also significant. In a second experiment, thetime for spoilage by this microorganism in concentrated grape juice was evaluated at isothermal (23 C)and non-isothermal conditions, in an effort to reproduce standard storage and overseas shipping temperatureconditions, respectively. Results show that pH was again the environmental factor with the highest impact on delaying the spoilage of the product. Thereby, a pH value below 2.0 was enough to increase the shelf life of the product for more than 60 days in both isothermal and non-isothermal conditions. The information obtained in the present work could be used by producers and buyers to predict the growth and time for spoilage of Z. rouxii in concentrated grape juice.","palabras_clave":"ZYGOSACCHAROMYCES ROUXII|CONCENTRATED GRAPE JUICE|SURFACE RESPONSE|SPOILAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1055,"idioma_id":4,"titulo":"TECHNOLOGICAL EVOLUTION OF HORMONE DELIVERY SYSTEMS FOR ESTROUS SYNCHRONIZATION IN CATTLE","resumen":"  \t&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;!--  \t\t@page { margin: 2cm }  \t\tP { margin-bottom: 0.21cm; direction: ltr; color: #00000a; text-align: left; widows: 0; orphans: 0 }  \t\tP.western { font-family: 'Nimbus Roman No9 L', 'Times New Roman', serif; font-size: 12pt; so-language: en-US }  \t\tP.cjk { font-family: 'DejaVu Sans'; font-size: 12pt; so-language: zh-CN }  \t\tP.ctl { font-family: 'Nimbus Roman No9 L', 'Times New Roman'; font-size: 12pt; so-language: ar-SA }  \t\tA:link { so-language: zxx }  \t--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;  \t                        \t  \t  \t  \t  \t&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;!--  \t\t@page { margin: 2cm }  \t\tP { margin-bottom: 0.21cm; direction: ltr; color: #00000a; text-align: left; widows: 0; orphans: 0 }  \t\tP.western { font-family: 'Nimbus Roman No9 L', 'Times New Roman', serif; font-size: 12pt; so-language: en-US }  \t\tP.cjk { font-family: 'DejaVu Sans'; font-size: 12pt; so-language: zh-CN }  \t\tP.ctl { font-family: 'Nimbus Roman No9 L', 'Times New Roman'; font-size: 12pt; so-language: ar-SA }  \t\tA:link { so-language: zxx }  \t--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;This  paper reviews the evolution of hormone delivery systems and discuss  future technological trends in the field. From intravaginal devices  to recent advances in nano and microcapsules will be reviewed. The  description of delivery systems manufacture processes, their  advantages and disadvantages and the reasons for their success and  eventual decline will be described. The increment of the Global  demand of animal products, the need for cost reduction, the limited  fertile land, the competition with agriculture and the preservation  of biodiversity were identified as the driving forces for  technological evolution of hormone delivery systems. Micro  and nanoencapsulation processes, biomaterials and subcutaneous  delivery routes dominate actual research topics. Neuronal targeted  drugs administrated through non-invasive routes are speculated as one  of the most promissing future drug delivery system for estrous  synchronization in livestock.","palabras_clave":"DRUG DELIVERY|ANIMAL REPRODUCTION|ARTIFICIAL INSEMINATION|OVULATION|MICROTECHNOLOGY|LIVESTOCK"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1056,"idioma_id":5,"titulo":"Cóndor 2, Un sitio del periodo del Desarrollos Regionales 1 en el sector norte de la Quebrada de Humahuaca, Jujuy","resumen":"En este trabajo se presentan los primeros resultados de las investigaciones arqueológicas recientemente iniciadas en la Quebrada de Cóndor (Dpto. Humahuaca, Jujuy), una quebrada alta tributaria del río Grande, localizada en el sector norte de la Quebrada de Humahuaca, que no cuenta con antecedentes de investigación previos. En este trabajo nos concentramos en el sitio Cóndor 2, que se ubica en la terraza fluvial de la margen derecha del arroyo Cóndor, en el tramo superior de la quebrada. Si bien los dinámicos procesos geomorfológicos locales han cubierto completamente el sitio, diversos vestigios de ocupación humana son, debido a la acción de proceso erosivos naturales, claramente visibles en la barranca del arroyo Cóndor. La intervención arqueológica en los perfiles expuestos (limpieza, refilado y excavación) ha permitido determinar la existencia de dos ocupaciones bien definidas, separadas por eventos de sedimentación aluvial. Dichas ocupaciones han sido fechadas radiocarbónicamente en 1130 y 960 A.P. respectivamente, lo que las sitúa en el Período de Desarrollos Regionales 1 (ca. 900-1250 d.C.) de la secuencia regional. Se presenta aquí la descripción de los diversos materiales recuperados, avanzándose en la caracterización preliminar de ambas ocupaciones y  situándolas en el marco del contexto cultural regional.","palabras_clave":"QUEBRADA DE CÓNDOR  |PERÍODO DE DESARROLLOS REGIONALES 1 |SECTOR NORTE DE LA QUEBRADA DE HUMAHUACA|PROCESOS GEOMORFOLÓGICOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1057,"idioma_id":4,"titulo":"Reassessment of mid-Carboniferous glacial extent in southwestern Gondwana (Rio Blanco Basin, Argentina) inferred from paleo-mass transport of diamictites","resumen":"Late Paleozoic glacial diamictites occur in many localities in western Argentina,indicating that the region was strongly affected by glaciation during the mid-Carboniferous (late Serpukhovian-early Bashkirian). In most instances these diamictitesare found in steeply walled paleovalley settings in the Andean Precordillera. This studypresents new data from a locality north of the Precordillera that suggests an additional,distinct, volume of ice existed in the region during the Carboniferous. The glacigenicdiamictites in the Rio Blanco Basin were ultimately emplaced as gravity flows,precluding inferences of paleo-ice volume. Fold nose orientation and soft-sedimentgroove orientations within the diamictites indicate that the deposits were emplaced fromnorth to south, suggesting that glacial ice was most likely not sourced from the proto-Precordillera at this locality, requiring the need for another ice center to the north of thebasin. Diamictite facies indicates that the sediment was initially supplied to the study areaby a warm-based glacier.","palabras_clave":"CARBONIFEROUS|PRECORDILLERA|TILLITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1058,"idioma_id":4,"titulo":"GEOGRAPHICAL ORIGIN CLASSIFICATION OF ARGENTINEAN HONEYS USING A DIGITAL IMAGE-BASED FLOW-BATCH SYSTEM","resumen":"In this paper a geographic origin classification of honey samples from Argentina was proposed. For this reason,a conventional flow-batch system with a simple webcam to capture digital images was employed. In thismethodology, analytical information is generated from color histograms obtained from the digital imagesemploying different color models (RGB (red?green?blue), HSB (hue?saturation?brightness) and Grayscale).Three chemometric tools were employed for geographic origin classification (SPA-LDA (successive projectionsalgorithm-linear discriminant analysis), SIMCA (soft independent modeling for class analogy), and PLS-DA(partial least squares-discriminant analysis)). The proposedmethod is a good option to be used in quality controllaboratories for the classification of honey samples according to their geographical origin.","palabras_clave":"HONEY|CLASSIFICATION|GEOGRAPHICAL ORIGIN|FLOW BATCH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1059,"idioma_id":4,"titulo":"Acellular pertussis vaccine based on outer membrane vesicles capable of conferring both long-lasting immunity and protection against different strain genotypes","resumen":"Despite high vaccination coverage rates, pertussis continues to be a global concern, with increased incidence widely noted. The current pertussis epidemiologic situation has been mainly attributed to waning immunity and pathogen adaptation. To improve the disease control, a new generation of vaccines capable to overcome those weaknesses associated to the current vaccines need to be developed. Previously we have demonstrated that the outer membrane vesicles obtained from the recombinant Bordetella pertussis strain expressing PagL enzyme (OMVs(BpPagL)) are good vaccine candidates to protect against pertussis. In this work the OMVs(BpPagL) formulated with diphtheria and tetanus toxoids (Tdap(OMVsBpPagL)) was used to evaluate its capacity to offer protection against Argentinean clinical isolates and to induce long-term immunity. To these aims BALB\/c mice were immunized with Tdap(OMVsBpPagL) and challenged with sublethal doses of the clinical isolate Bp106 selected as a representative circulating isolate. Comparisons with a current commercial Tdap vaccine used at a dose in which pertussis toxin level was equivalent to that of Tdap(OMVsBpPagL) were performed. With the normalized doses of both vaccines we observed that Tdap(OMVsBpPagL) protected against the clinical isolate infection, whereas current commercial Tdap vaccine showed little protection against such pathogen. Regarding long-term immunity we observed that the Tdap(OMVsBpPagL) protective capacity against the recommended WHO reference strain persisted at least 9 months. In agreement with these results Tdap(OMVsBpPagL) induced Th1 and Th2 immune response. In contrast, commercial Tdap induced Th2 but weak Th1 responses. All results presented here showed that Tdap(OMVsBpPagL) is an interesting formulation to be considered for the development of novel acellular multi-antigen vaccine.","palabras_clave":"ACELLULAR VACCINE|PERTUSSIS|OUTER MEMBRANE VESICLES|WHOOPING COUGH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1063,"idioma_id":4,"titulo":"'Autophagy in inflammation, infection, neurodegeneration and cancer'","resumen":"In its classical form, autophagy is an essential, homeostatic process by which cytoplasmic components are degraded in a double-membrane-bound autophagosome in response to starvation. Paradoxically, although autophagy is primarily a protective process for the cell, it can also play a role in cell death. The roles of autophagy bridge both the innate and adaptive immune systems and autophagic dysfunction is associated with inflammation, infection, neurodegeneration and cancer. In this review, we discuss the contribution of autophagy to inflammatory, infectious and neurodegenerative diseases, as well as cancer.","palabras_clave":"ATG|AUTOPHAGY|BECLIN-1|NEUROINFLAMMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1064,"idioma_id":4,"titulo":"Reproductive biology of the Magellan skate, Bathyraja magellanica (Chondrichthyes, Rajidae), in the Southwest Atlantic","resumen":"The knowledge of reproductive parameters is essential to develop effective management and conservation  plans. The present study provides the first information on the reproductive biology of an abundant and important trawl  by-catch skate, Bathyraja magellanica, along its geographic range in the south-western Atlantic (43?558S). In total,  81 males (205?738 mm total length, TL) and 104 females (157?768 mm TL) were analysed. Length?frequency  5 distributions were significantly different between sexes, with females being larger than males. However, no sexual  dimorphism was observed in TL?total body weight relationship and liver size. Length at maturity was similar between  males (638 mm TL) and females (653 mm TL), ,85% of maximum observed size. The mean number of vitellogenic  oocytes per skate was 14.37 (4.43), increasing with female TL. Egg cases ofB.magellanica had striated surface, broad  lateral keels, extremely long posterior horns, and its wall consisted of three layers. An important area for the reproduction  10 of B. magellanica was found between 488500  S and 508300  S. The present results indicated that this species has a low  reproductive efficiency, suggesting that the B. magellanica population should be monitored closely to avoid large-scale  changes in skate community structure of the south-western Atlantic","palabras_clave":"EGG CASES|EGG-LAYING AREA|ELASMOBRANCHS|MATURITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1065,"idioma_id":4,"titulo":"Spatial distribution of cephalopod paralarvae in San José Gulf (Northern Patagonia, Argentina): the role of tidal circulation in larval dispersal","resumen":"The spatial distribution of cephalopod paralarvae was studied in waters of San José Gulf (SJG), a shallow basin connected to a larger gulf (San Matías, SMG) through a narrow mouth located on its northwestern margin. The gulf is characterized by a remarkable tidal circulation pattern providing contrasting hydrodynamic conditions between the western and eastern domains. Zooplankton net tows (n = 104) and depth-temperature profiling (n = 70) were conducted on a monthly basis at fixed stations forming a regular grid from October (mid spring) 2011 to March (late summer) 2012. Temperature-depth profiles showed that while the western domain seawater column remains well mixed by tidal currents during the whole period, stratification develops from November to March on the eastern domain. Positive zooplankton net tows were sporadic for early-life stages of Semirossia tenera (n = 1) and Robsonella fontaniana (n = 3), and more frequent for those of Doryteuthis sanpaulensis (n = 9) and Illex argentinus (n = 9). The latter were found almost exclusively in the western domain whereas those from D. sanpaulensis were mainly sampled in the eastern domain. Low abundance of I. argentinus paralarvae in the eastern domain suggests that buoyant egg masses of this squid are not released in SJG; instead they, or the hatching paralarvae emerging from them, could be sequentially advected from and expelled to SMG by tidal currents. On the other hand, the spatial distribution of D. sanpaulensis paralarvae suggests that those hatching on the shallow marginal bottoms of the eastern domain are retained in this region while those hatching at the western domain are flushed out to SMG by tidal currents.","palabras_clave":"Fine-scale spatial distribution|Early-life stages dispersion|Coastal waters|Water temperature profiles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1066,"idioma_id":4,"titulo":"Phenol methylation on acid catalysts: Study of the catalyst deactivation kinetics and mechanism","resumen":"          The kinetics and mechanism of coke formation and  catalyst deactivation during the synthesis of cresols from phenol methylation  were investigated on SiO2-Al2O3,  tungstophosphoric acid (HPA) supported on silica, and zeolites HBEA, HZSM5,  HMCM22 and HY. The nature, density and strength of surface acid sites were  probed by temperature programmed desorption of NH3 coupled with  infrared spectra of adsorbed pyridine. Coke formed on the catalysts during reaction was characterized by  temperature programmed oxidation. All the samples deactivated on stream. A  linear correlation was observed between the initial catalyst deactivation and  the amount of coke, thereby indicating that coke formation was responsible for  the activity decay. Coking kinetic studies showed that a significant part of  coke was rapidly formed from the reactants. When methanol was feeding alone,  significant amounts of carbon were formed on the catalysts (between 0.6 and 6.2  %C), particularly on samples containing mainly strong Brønsted acid sites. Nevertheless,  the coke amounts formed during phenol methylation were clearly higher (between  3.7 and 14.9 %C), which showed that phenol was also responsible for coke  formation. More insight on the role of phenol and methanol on coke formation  was obtained by characterizing the coke nature using infrared spectroscopy.  Coked samples recovered after methanol decomposition reaction exhibited IR  absorption bands characteristics of olefinic species formed on Brønsted and  Lewis acid sites. The IR spectra of coked samples recovered after phenol  methylation showed the presence of phenolate, aromatic and polyaromatic  species&nbsp;&nbsp; adsorbed mainly on Lewis acid  sites.  ","palabras_clave":"PHENOL METHYLATION|CRESOL SYNTHESIS|ACID CATALYSTS|CATALYST DEACTIVATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1067,"idioma_id":4,"titulo":"Agronomic performance and Essential Oil Composition of Ocimum basilicum L.: Effect of Genotype and Date of Harvest","resumen":"        Un estudio en Ocimum basilicum  L.(albahaca) con dos genotipos (verde y morado) se llevo a cabo para determinar  el comportamiento productivo del cultivo y&nbsp;  la&nbsp; composición y estabilidad&nbsp; de compuestos aromáticos en&nbsp; ciclo otoño-inviernal, bajo cubierta en  sistema hidropónico. Para estudiar los aspectos productivos se aplicaron  tratamientos según genotipo verde y morado. Para el estudio de composición de  aceite y su estabilidad, los tratamientos aplicados fueron según genotipo y  fecha de cosecha (primera y segunda cosecha). Se realizo una poda entre las  últimas cosechas. Los resultados indican una gran diferencia en&nbsp; parámetros productivos como peso fresco y  seco (g.planta-1), rendimiento (g.planta-1) y tasa de  crecimiento absoluta (g.dia-1) y relativa (g. g-1.dia-1),  en relación a un cultivo de época óptima. La poda, si bien provoca  rejuvenecimiento, causó un rebrote y recuperación menor en el genotipo morado.  La composición de aceite esencial varía para ambos genotipos y según la fecha  de cosecha. El linalol predomina en la primera parte del ciclo con&nbsp; condiciones ambientales favorables y en  albahaca verde.&nbsp; El metil-eugenol&nbsp; aumenta para la segunda fecha de cosecha,  marcadamente en albahaca morada. La radiación y temperatura con tendencia  decreciente durante el ciclo influyen en la producción de biomasa y la  composición de aceite esencial. El genotipo verde tiene un mejor comportamiento  productivo durante todo el ciclo. La estabilidad entre cosechas sucesivas varía  para ambas variedades. Si bien hay diferencia entre genotipos en composición de  aceite, se cree que la poda habría influido marcadamente en la composición de  la variedad morada. Este estudio demostró que es posible realizar el cultivo en  época desfavorable bajo cubierta. A pesar de que el rendimiento se resiente se  pudieron obtener&nbsp; plantas con calidad  aromática elevada.  ","palabras_clave":"Ocinum basilicum|rendimiento|genotipo|aceites esenciales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1068,"idioma_id":4,"titulo":"A comparative study of zirconium and titanium implants in rat: Osseointegration and bone material quality","resumen":"Permanent metal implants are widely used in  human medical treatments and orthopedics, for example as  hip joint replacements. They are commonly made of titanium  alloys and beyond the optimization of this established  material, it is also essential to explore alternative implant  materials in view of improved osseointegration. The aim of  our study was to characterize the implant performance of  zirconium in comparison to titanium implants. Zirconium  implants have been characterized in a previous study  concerning material properties and surface characteristics  in vitro, such as oxide layer thickness and surface roughness.  In the present study, we compare bone material  quality around zirconium and titanium implants in terms of  osseointegration and therefore characterized bone material  properties in a rat model using a multi-method approach.  We used light and electron microscopy, micro Raman  spectroscopy, micro X-ray fluorescence and X-ray scattering  techniques to investigate the osseointegration in  terms of compositional and structural properties of the  newly formed bone. Regarding the mineralization level, the  mineral composition, and the alignment and order of the  mineral particles, our results show that the maturity of the  newly formed bone after 8 weeks of implantation is  already very high. In conclusion, the bone material quality  obtained for zirconium implants is at least as good as for  titanium. It seems that the zirconium implants can be a  good candidate for using as permanent metal prosthesis for  orthopedic treatments.","palabras_clave":"TITANIUM|ZIRCONIUM|IMPLANTS|OSEOINTEGRATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1069,"idioma_id":4,"titulo":"Geographical differentiation in floral traits along the distribution range of the Patagonian oil-producing Calceolaria polyrhiza: do pollinators matter?","resumen":"Background and Aims: The underlying evolutionary and ecological processes of pollinator driven floral diversification are still poorly understood. The Grant-Stebbins model suggests that differences in geographical ranges of the more effective pollinator could result in ?pollination ecotypes?. To test this hypothesis, we studied geographical phenotypic  variation of Calceolaria polyrhiza and its pollinators, two oil-collecting bee species that differ significantly in body size and geographical distribution. This system conform a specialist oil-flower\/oil-bee plant-pollinator interaction endemic to Patagonia.    Methods: We measured six floral and seven vegetative traits in 46 populations distributed across the entire species range. We selected 24 sites and retrieved their climatic and edaphic parameters; we captured 2-16 bees\/site of the most frequent pollinator species and measured a critical flower-bee fitting trait involved in effective pollination. We examined geographical pattern of phenotypic variation and its relationship with biotic and abiotic factors using uni- and multivariate analyses. Mantel permutation test was used to explore  decoupled geographic variation between corolla area and fit-related floral traits.   Key Results: Body length of pollinators and floral fit-related traits were strongly correlated  with one another. Geographic variation of mechanical-fit related traits is decoupled from  variation in corolla size. This latter floral trait has a geographical pattern concurrent with that of vegetative traits and is mainly affected by climatic gradients.   Conclusions: The relationship between floral operative distance and bee length remained significant even in models that included various environmental variables and an allometric predictor one. We conclude that in this system pollinators play a key role in shaping floral phenotype at a geographical scale, promoting the differentiation of two floral ecotypes. The abiotic environment also has an important role, mainly affecting floral size. Decoupled geographical variation between fit-related traits and floral size is suggested to represent a strategy to maintain plant-pollinator phenotypic matching in this environmentally heterogeneous area.","palabras_clave":"Abiotic environmental gradients|Calceolaria polyrhiza|Centris|Chalepogenus|coupled\/decoupled variation|floral ecotypes|local adaptation|oil-collecting  8  bees|Patagonia|specialized pollination|vegetative morphology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1070,"idioma_id":4,"titulo":"Electrochemical ultra-micro sensors  for the determination of synthetic and natural antioxidants in edible vegetable oils","resumen":"Edible vegetable oils Tocopherols Tert-butyl hydroxytoluene Square wave voltammetry Ultramicroelectrodes a b s t r a c t We describe the application of square wave voltammetry at ultramicroelectrodes for the determination of natural antioxidants (&#9251;, &#9254;, and &#9253; tocopherols), and tert-butyl hydroxytoluene in edible vegetable oils. Tocopherol determinations were performed in benzene\/ethanol (1:2) + 0.1 mol L &#8722;1 H 2 SO 4 + oil samples at a carbon fiber disk ultramicroelectrode, and tert-butyl hydroxytoluene was determined in acetonitrile (ACN) + 0.1 mol L &#8722;1 (C 4 H 9) 4 NF 6 P at a Pt band ultramicroelectrode after performing its extraction from the oil sample with ACN. Recovery percentages determined by the standard additions method were in the range from 92% to 102%, with variation coefficients between 0.5% and 4%. Antioxidant concentrations calculated by this methodology were in good agreement with those values declared by the manufacturers.","palabras_clave":"EDIBLE VEGETABLE OILS|TOCOPHEROLS|SQUARE WAVE VOLTAMMETRY|ULTRAMICROELECTRODES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1071,"idioma_id":4,"titulo":"Prey selection and food habits of breeding Burrowing Owls (Athene cunicularia) in natural and modi fi ed habitats of Argentine pampas","resumen":"Understanding the way animals respond to novel habitats and fluctuations in food resources can provide insights into speciesâ?? abilities to settle in modified habitats. We studied the patterns of arthropod selection by the Burrowing Owl in natural and modified habitats. We assessed the diet of this owl by means of pellet analysis and, simultaneously, we estimated arthropod field relative abundance by trapping. A similar pattern of prey selection and avoidance was found among habitats, being coleopterans the main prey consumed. Food-niche breadth was relatively low at all habitats, reflecting the dominance of a few prey items in the diet of the Burrowing Owl. Its wide trophic spectrum as well as its ability to take advantage of dominant resources suggests that from a feeding perspective the Burrowing Owl has no limitations to colonize and adapt to new human-modified habitats.","palabras_clave":"No consigna"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1072,"idioma_id":4,"titulo":"Physiological stratification in electricity-producing  biofilms of Geobacter sulfurreducens","resumen":"The elucidation of mechanisms and limitations in electrode respiration by electroactive biofilms is significant for the development of rapidly emerging clean energy production and wastewater treatment technologies. In Geobacter sulfurreducens biofilms, the controlling steps in current production are thought to be the metabolic activity of cells, but still remain to be determined. By quantifying the DNA, RNA, and protein content during the long-term growth of biofilms on polarized graphite electrodes, we show in this work that current production becomes independent of DNA accumulation immediately after a maximal current is achieved. Indeed, the mean respiratory rate of biofilms rapidly decreases after this point, which indicates the progressive accumulation of cells that do not contribute to current production or contribute to a negligible extent. These results support the occurrence of physiological stratification within biofilms as a consequence of respiratory limitations imposed by limited biofilm conductivity.","palabras_clave":"biological activity|DNA|electrochemistry|energy conversion;fuel cells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1073,"idioma_id":4,"titulo":"Dual role of astrocytes in perinatal asphyxia injury and neuroprotection","resumen":"Perinatal asphyxia represents an important cause of severe neurological deficits including delayed mental and motor development, epilepsy, major cognitive deficits and blindness. However, at the moment, most of the therapeutic strategies were not well targeted toward the processes that induced the brain injury during perinatal asphyxia. Traditionally, experimental research focused on neurons, whereas astrocytes have been more related with the damage mechanisms of perinatal asphyxia. In this work, we propose to review possible protective as well as deleterious roles of astrocytes in the asphyctic brain with the aim to stimulate further research in this area of perinatal asphyxia still not well studied.","palabras_clave":"PERINATAL ASPHYXIA|ASTROCYTES|NEUROPROTECTION|REDOX SIGNALLIG|CELL DEATH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1075,"idioma_id":4,"titulo":"Analysis of a chitinase from EpapGV, a fast killing betabaculovirus","resumen":"The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA from Epinotia aporema Granulovirus (EpapGV) was identified and characterized. The 1,713 base pairs long open reading frame encodes a protein of 570 amino acids with a predicted molecular weight of 63 kDa. EpapGVV-CHIA sequence alignment resulted 62 % identical to Pieris rapae GV and Blastp search revealed a high conservation among all baculovirus chitinases. Amino acid sequence analysis indicated that the C-terminal KDEL present in most alphabaculovirus chitinases is absent in EpapGV V-CHIA, as well as in the rest of the betabaculoviruses. Phylogenetic analysis was performed with bacterial, lepidopteran, and baculoviral chitinase sequencesavailable in databases. Using an AcMNPV bacmid (bAp-GOZA) a recombinant Ac-chiAEpapGV was obtained in order to overexpress EpapGV V-CHIA in cell culture. The presence of chitinase was detected in purified AcMNPVchiAEpapGV OBs. Peritrophic membranes of Anticarsia gemmatalis larvae fed with recombinant OBs showed an altered structure. The results presented in this study show that EpapGV chitinase overexpression in recombinant baculovirus can cause association of this protein with OBs, and suggest that this could be used to evaluate the protein role in early stages of baculoviral infections.","palabras_clave":"chitinase|EpapGV|Baculovirus|peritrophic membrane"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1077,"idioma_id":4,"titulo":"Growth responses and ion accumulation in the halophytic  legume Prosopis strombulifera are determined by Na2SO4 and NaCl","resumen":"Halophytes are potential gene sources for genetic manipulation of economically important&nbsp;crop species. This study addresses the physiological responses of a widespread halophyte, Prosopis strombulifera (Lam.) Benth to salinity. We hypothesised that increasing&nbsp;concentrations of the two major salts present in soils of central Argentina&nbsp;(Na2SO4, NaCl, or their iso-osmotic mixture) would produce distinct physiological&nbsp;responses. We used hydroponically grown P. strombulifera to test this hypothesis,&nbsp;analysing growth parameters, water relations, photosynthetic pigments, cations&nbsp;and anions. These plants showed a halophytic response to NaCl, but strong general&nbsp;inhibition of growth in response to iso-osmotic solutions containing Na2SO4.&nbsp;The explanation for the adaptive success of P. strombuliferain high NaCl conditions seems to be related to a delicate balance between Na+ accumulation&nbsp;(and its use for osmotic adjustment) and efficient compartmentalisation in vacuoles,&nbsp;the ability of the whole plant to ensure sufficient K+ supply by maintaining high K+\/Na+discrimination, and maintenance of normal Ca2+ levels in leaves. The three salt treatments had different effects on the accumulation of ions.&nbsp;Findings in bi-saline-treated plants were of particular interest, where most of the physiological parameters studied showed partial alleviation of SO42--induced toxicity by Cl-. Thus, discussions on physiological responses to salinity could be further expanded in a way that more closely mimics natural salt environments.","palabras_clave":"CHLORIDE|HALOPHYTES|SALT MIXTURE|SULPHATE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1078,"idioma_id":4,"titulo":"Distribution of (-)-Hamanasic Acid A in South American Species of Flourensia and Phytotoxic Effects of Leaf Aqueous Extracts","resumen":"The presence of the phytotoxic sesquiterpene (-)-hamanasic acid A {(-)HAA; 7-carboxy-8-hydroxy-1(2), 12(13)-dien-bisabolene} isolated from Flourensiacampestris (FC), was investigated in the South American species of the genus, together with the evaluation of the phytotoxic activity of their leaf aqueous extracts. (-)HAA was identified and isolated from F. fiebrigii (FF) and F. oolepis (FO), being chemically (GC-MS, NMR, [á]D) and biologically (bioassayed on lettuce) indistinguishable from that of FC, while no (-)HAA was found in F. hirta (FH), F. riparia (FR) and F. niederleinii (FN). Its leaf content in FF was similar to that found in FC (ca. 15 mg g-1 WT) and significantly higher than in FO (0.8 mg g-1 WT). The screening for the presence of (-)HAA in other species of Flourensia communities showed that its natural occurrence is restricted only to Flourensiaspecies. No (-)HAA could be detected in any of the 37 -most representative- species of these communities (26 natives, 11 exotics), despite many of them belonging to the same family and tribe as Flourensia spp. Leaf aqueous extracts of all Flourensia species exhibited strong inhibitory effects on lettuce germination and on root and shoot growth, regardless of the presence and content of (-)HAA. These results strongly suggest the existence of other powerful phytotoxic compounds in those Flourensiaspp lacking (-)HAA. Our results clearly show that (-)HAA only pertains to some species of the genus Flourensia. Relative to previous exomorphologic groupings of the genus, our chemotaxonomic data would give support to the close link described between FC and FF, but not with FR. In addition, the fact that (-)HAA was also found in FO, which belongs to a second different line, also points out that species position in this lineage would deserve to be revisited. The restricted production of (-)HAA by Flourensia in their communities suggests its special link with the genus, and sustains its putative allelochemical role.","palabras_clave":"FLOURENSIA|(-) HAMANASIC ACID A|PHYTOTOXIC ATTIVITY|SESQUITERPENES|CHEMOTAXONOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1079,"idioma_id":4,"titulo":"Bionomics of Opius bellus (Hymenoptera: Braconidae), an endoparasitoid of Anastrepha fraterculus (Diptera: Tephritidae) in fruit growing areas of Northwestern Argentina","resumen":"Opius bellus is a neotropical larval-prepupal parasitoid known to attack thepestiferous fruit fly, Anastrepha fraterculus. Due to interest in the use of nativeparasitoids in forthcoming fruit fly biocontrol programmes in Argentina, O. belluswas colonised for the first time using laboratory-reared A. fraterculus larvae. A20 series of experiments were conducted to (1) best achieve an efficient parasitoidrearing by determining optimal larval host age, host:parasitoid ratio and hostexposure time and (2) assess their potential as biological control agents bydetermining reproductive parameters. The most productive exposure regimen was:7?9 d-old (early and middle third-instars) A. fraterculus larvae for 4 h at a 4:1 host:25 parasitoid ratio; this array of factors was sufficient to achieve the highest averageadult emergence (48%) and an offspring sex ratio at equitable proportion.Increasing both host:parasitoid ratio further than 4:1 and the host exposuretime beyond 4 h did not significantly enhance parasitoid female offspring yield.Females produced eggs for 29.5 ± 1.4 days. At 32 days of age, 50% of the females30 were still alive. The majority of the progeny were produced by females between 20and 24 d-old. At 26°C, gross fecundity rate, net reproductive rate, intrinsic rate ofincrease and mean generation time were 20.7 ± 4.2 offspring\/female, 9.6 ± 2.5females\/newborn females, 0.06 ± 0.01 females\/female\/day and 8.4 ± 0.2 days,respectively. The long lifespan and reproductive parameters suggest that this35 parasitoid species has suitable attributes for mass-rearing.","palabras_clave":"FRUIT FLY|PARASITOID|BRACONIDAE|REARING|DEMOGRAPHIC PARAMETERS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1080,"idioma_id":4,"titulo":"Active speed role in opinion formation of interacting moving agents","resumen":"            We propose a general non-linear analytical framework to study the  evolution of the opinion state of a population of moving individuals. This  novel scheme allows us to study a broad range of social phenomena, like, for  example, the influence of agent interaction dynamics in the opinion formation  or the inclusion of different individual?s idiosyncrasies. We consider  societies composed by agents who adopt one of the n possible opinions or  internal states. The opinion state may only be modified while the agent keeps  contact with another one. In general, this framework could be solved numerically,  and, for some special perturbative cases, it is possible to find analytical  steady states. In order to check our scheme for different social conventions, we  implement computational simulations of an ensemble of self-propelled agents,  finding a good agreement between theory and simulation results. We found, for  slow society kinetics in all the cases studied, that there exist a shift of the  opinion populations towards the moderate opinion states. This suggest that  active speed can be understood as a parameter measuring the social temperature  of the community.  ","palabras_clave":"Stochastic processes|Dynamics of social systems|Nonlinear dynamics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1081,"idioma_id":4,"titulo":"Genetic basis of triatomine behavior: lessons from available insect genomes.","resumen":"Triatomines have been important model organisms for behavioural research. Diverse reports about triatomine host search, pheromone communication in the sexual, shelter and alarm contexts, daily cycles of activity, refuge choice and behavioural plasticity have been published in the last two decades. In recent times, a variety of molecular genetics techniques has allowed researchers to investigate elaborate and complex questions about the genetic bases of the physiology of insects. This, together with the current characterisation of the genome sequence of Rhodnius prolixus allows the resurgence of this excellent insect physiology model in the omics era. In the present revision, we suggest that studying the molecular basis of behaviour and sensory ecology in triatomines will promote a deeper understanding of fundamental aspects of insect and, particularly, vector biology. This will allow uncovering unknown features of essential insect physiology questions for a hemimetabolous model organism, promoting more robust comparative studies of insect sensory function and cognition.","palabras_clave":"Behaviour|Sensory physiology|Olfaction|Genes|Triatomines"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1082,"idioma_id":5,"titulo":"Nuevos registros de Leptodactylus furnarius Sazima & Bokermann 1978 (Anura: Leptodactylidae) en Argentina","resumen":"En el presente trabajo presentamos una actualización para la distribución de L. furnarius, con cuatro nuevas localidades, dos procedentes de la provincia de Misiones y dos de la provincia de Corrientes; siendo éstos los primeros registros para la provincia. Estas citas amplían la distribución conocida de L. furnarius más de 90 kilómetros al oeste del único registro conocido en Argentina. Como fuera señalado previamente, aunque la distribución de L. furnarius refleja cierta discontinuidad, este hecho podría deberse simplemente a una escasez en el esfuerzo de muestreo y a la dificultad para capturarla.","palabras_clave":"Leptodactylus furnarius| Corrientes|Misiones|distribución geográfica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1086,"idioma_id":5,"titulo":"ADAPTACIÓN DE UN MODELO GRAVIMÉTRICO TRIDIMENSIONAL AL ÁREA DEL MACIZO NORPATAGÓNICO","resumen":"El Macizo Norpatagónico es una altiplanicie que casi no presenta deformación rodeada de cuencas con distintos grados de deformación terciaria sobre las cuales sobresale entre 500 y 700 metros. Dicho macizo sufrió un levantamiento repentino de más de 1200 m en el Paleógeno, hipotéticamente, a causa de movimientos epirogénicos. Esta última afirmación se supone de este modo ya que se puede observar la presencia de unidades formacionales de origen marino no deformadas en el macizo, las cuales se pueden encontrar en las cuencas circundantes con distintos grados de deformación. Con el fin de aportar al conocimiento acerca de la formación y el estado actual de dicha zona, se realizó la adaptación de un modelo gravimétrico tridimensional (Tassara et al., 2006 y Tassara y Echaurren, 2011) al área del Macizo Norpatagónico. Para realizar la adaptación y el análisis de los resultados se utilizó el programa de modelado gravimétrico y magnético tridimensional e interactivo IGMAS+ (Götze, 1978 y 1984, Götze y Lahmeyer 1988 y Schmidt y Götze, 1998) y se extrajeron datos de anomalías de Bouguer del modelo geopotencial EGM2008 (Pavlis et al., 2008). Se observó un buen ajuste entre las superficies calculada y observada para el modelo. Se extrajo y mapeó la superficie de la discontinuidad de Mohorovicic para el modelo, pudiéndose notar una corteza de espesor aproximadamente constante y superior a los 36 km. para el área del macizo rodeada de zonas de espesor cortical inferior hacia el Este y Norte mientras que en su límite Oeste se observan áreas de corteza más potente. La adaptación deja una pequeña zona del sudeste del macizo fuera del modelo con lo cual no se tiene información de lo que ocurre en el límite sur del mismo","palabras_clave":"Gravimetia|Modelado|Patagonia|Macizo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1087,"idioma_id":5,"titulo":"Escherichia coli con \t\t\tresistencia a múltiples antimicrobianos en granjas de producción porcina de la República Argentina","resumen":"Resumen: Los objetivos del presente trabajo fueron: i) monitorear la resistencia de E. coli frente a diversos antimicrobianos frecuentemente utilizados con fines  terapéuticos y profilácticos en explotaciones porcinas; ii) aislar y caracterizar fenotípica y genotípicamente E. coli toxigénicos provenientes de cerdos con diarrea pre y posdestete; iii) determinar la presencia de integronesclase 1 y 2 como posible mecanismo de diseminación de resistencia. Se procesaron 216 hisopados rectales de cerdos clínicamente sanos y con diarrea, de 15 granjas de producción porcina. El 46,6 % de los aislamientos presentó resistencia a múltiples antimicrobianos. El 93 % fueron resistentes a tetraciclina, el 59 % a ciprofloxacina, el 52 % a florfenicol, 8 % a amoxicilina\/ác. clavulánico y 0,6 % a gentamicina. No se observó resistencia a colistina. De 56 E. coli, 34 portaron al menos uno de los genes int1 o int2. Se aisló E. coli toxigénico a partir del 53 % de los cerdos con diarrea. El uso inadecuado de antimicrobianos con finesprofilácticos o terapéuticos en medicina veterinaria, implica un riesgo para la salud pública.","palabras_clave":"Escherichia coli|Resistencia antimicrobiana|Integrones|Cerdos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1088,"idioma_id":4,"titulo":"Memory destabilization is critical for the success of the reactivation extinction procedure","resumen":"It has been suggested that, unlike pure extinction which typically results in the return of the fear response under a variety of  circumstances, memory reactivation followed by extinction can attenuate the reemergence of conditioned fear. The reactivation  extinction procedure has attracted the attention of basic and clinical researchers due to its potential clinical value for  the treatment of psychiatric conditions, such as anxiety and drug abuse disorders. However, mixed results have been achieved  so far in replicating and understanding this paradigm. It has been proposed that memory destabilization could be critical in  this sense. Using contextual fear conditioning in rats and midazolam as an amnesic agent, we first determined what reactivation  conditions are necessary to destabilize the mnemonic trace. After establishing the conditions for memory destabilization,  a series of experiments was conducted to determine if destabilization is critical for the success of the reactivation?extinction  procedure. Data confirmed the importance of memory destabilization prior to extinction inside the reconsolidation window  to attenuate spontaneous recovery and retard reacquisition of conditioned fear. The present report offers a candidate explanation  of the discrepancy in results obtained with the reactivation?extinction procedure by different laboratories.","palabras_clave":"MEMORY RECONSOLIDATION|FREE-DRUG PARADIGM|EXTINCTION|MIDAZOLAM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1089,"idioma_id":4,"titulo":"Solar oven with downward and upward solar gain for children?s dining rooms in developing countries","resumen":"In Argentina there exist children?s dining rooms where infants and their mothers take lunch atnoon and glasses of milk in the afternoon. These dining rooms are established in rural ormarginal urban areas. Solar ovens are an interesting option for cooking with less expense ofmoney, and they diminish environmental impact in rural zones. A solar oven with downwardand upward solar gain was built and tested. Upward solar gain is through Fresnel reflectors. Theuse of Fresnel reflectors resulted in higher heating power as compared to a solar oven withupper reflectors only. The performance with different numbers of Fresnel units was comparedwith a single-reflector solar oven with no lower Fresnel reflectors. Oven power was analysedand the result indicates that power grows as the number of Fresnel reflectors increases: it growsby 55% using 3 to 9 Fresnel reflectors, and by 34.6% with 3 to 6 Fresnel reflectors. Thesevalues are enough to cook several rations of food, and make this oven appropriate for use indining rooms in developing countries.","palabras_clave":"SOLAR  dining rooms, Developing countries.|OVEN|COOKER|FRESNEL|REFLECTOR|THERMAL |PERFORMANCE|TEST|CHILDREN |DINING|ROOM|DEVELOPING |COUNTRIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1091,"idioma_id":4,"titulo":"Impairing squamous differentiation by Klf4 deletion is sufficient to initiate tongue carcinoma development upon K-Ras activation in mice","resumen":"Impairing  squamous differentiation by Klf4 deletion is sufficient to initiate tongue  carcinoma development upon K-Ras activation in mice                        Abrigo M,  Alvarez R, Paparella M L, Calb D, Bal de Kier Joffe E, Gutkind J S and Raimondi  A R.                        Abstract                        Oral squamous  cell carcinoma (SCC) is among the most prevalent                        cancers in the  world and is characterized by high morbidity                        and few  therapeutic options. Like most cancers, oral SCC arises                        from a  multistep process involving alterations of genes responsible                        for balancing  proliferation and differentiation. Among these,                        Krûppel-like  factor 4 (Klf4) suppresses cell proliferation and                        promotes  differentiation and thus helps to maintain epithelial                        homeostasis.  However, the prevailing role of Klf4 in maintenance                        of normal  homeostasis in oral epithelium has not been established                        in vivo. Here,  we used an inducible oral-specific mice model                        to selectively  ablate Klf4 in the oral cavity. We generated K14-                        CreERTam\/Klf4f\/f  mice that survived to adulthood and did not present                        overt  phenotype. However, histologically these mice showed                        dysplastic  lesions, increased cell proliferation and abnormal differentiation                        in the tongue  4 months after induction, supporting                        a homeostatic  role of Klf4 in the oral epithelia. Furthermore,                        by breeding  these mutants with a transgenic line expressing at                        endogenous  levels K-rasG12D, we assessed the role of disrupting                        differentiation  gene programs to the carcinogenesis process. The                        K14-CreERTAM\/K-rasG12D\/Klf4&#8722;\/&#8722;  mice rapidly develop oral SCC                        in the tongue.  Thus, our findings support the emerging notion                        that  activation of differentiating gene programs may represent                        a barrier  preventing carcinogenesis in epithelial cells harboring                        oncogenic  mutations, and thus that molecules acting upstream                        and downstream  of Klf4 may represent components of a novel  tumor-suppressive  pathway.","palabras_clave":"Oral Cancer|KLF4|Ras"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1093,"idioma_id":4,"titulo":"QM\/MM study of the C-C coupling reaction mechanism of CYP121, an essential Cytochrome p450 of Mycobacterium tuberculosis","resumen":"Among 20 p450s of Mycobacterium tuberculosis (Mt), CYP121 has received an outstanding interest, not only due to its essentiality for bacterial viability but also because it catalyzes an unusual carbon?carbon coupling reaction. Based on the structure of the substrate bound enzyme, several reaction mechanisms were proposed involving first Tyr radical formation, second Tyr radical formation, and C?C coupling. Key and unknown features, being the nature of the species that generate the first and second radicals, and the role played by the protein scaffold each step. In the present work we have used classical and quantum based computer simulation methods to study in detail its reaction mechanism. Our results show that substrate binding promotes formation of the initial oxy complex, Compound I is the responsible for first Tyr radical formation, and that the second Tyr radical is formed subsequently, through a PCET reaction, promoted by the presence of key residue Arg386. The final C?C coupling reaction possibly occurs in bulk solution, thus yielding the product in one oxygen reduction cycle. Our results thus contribute to a better comprehension of MtCYP121 reaction mechanism, with direct implications for inhibitor design, and also contribute to our general understanding of these type of enzymes.","palabras_clave":"Tuberculosis|Bioinofrmatica|QM\/MM|Hemoproteinas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1094,"idioma_id":4,"titulo":"First molecular detection of Ramularia leaf spot (Ramularia collo-cygni) in seeds and leaves of barley in Argentina.","resumen":"The fungus Ramularia collo-cygni B. Sutton & J. M. Waller (Rcc) was identified as the causal agent of this emerging disease on barley (Hordeum vulgare L.) based on symptoms and signs on leaves and attributes of the fungus. The common name given to the disease was ?necrotic sprinkling?. This disease was found for the first time on barley in fields of Buenos Aires province, Argentina, in 2001with severities ranging from 60 to 100% (1). During the spring of 2012, the disease spread throughout most barley growing areas of the Pampean Region, affecting almost all varieties of barley in the Buenos Aires, Entre Ríos and Santa Fe provinces. The disease showed typical symptoms of small, brown spots on leaves, sheaths and awns and caused rapid loss of green leaf area and significant economic damage. The diagnosis of this disease is difficult by conventional techniques and has caused some confusion. In order to obtain appropriate information about the incidence of this pathogen in the most important barley growing region of Argentina, as well as to confirm its presence on seed, 39 seed samples, containing 200 seed each, and eight leaf samples were analyzed, using a real-time PCR diagnostic test (2). Thirty-five of the 37 seed samples had Rcc DNA levels above the minimum detection level (0.13 pg DNA). Ramularia has been described as a seed borne fungus previously (3) and seed infection could hasten the spread of the fungus to new geographically distinct areas. Moreover, leaf samples were microscopically assessed for conidophore presence and then ground for DNA extraction. Rcc DNA was detected by PCR in all eight leaf samples. To our knowledge this is the first report about molecular detection of Ramularia collo-cygni in barley seed of harvested samples from Argentina. Seeds infected\/infested may also lead to new strains of the fungus arriving in barley fields and provide a source of inoculum for future epidemics (4)","palabras_clave":"Ramularia collo-cygni|barley |Ramularia leaf spot|molecular detection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1095,"idioma_id":4,"titulo":"Stability of LaNi(5-x)Sn(x) cycled in Hydrogen","resumen":"In this work we study the effect of cycling in hydrogen with a purity grade 4.5 of six alloysbelonging to the LaNi5xSnx family for 0  x  0.5. Measurements consist in the alternaterepetition of absorption reactions at a temperature of 316 K and an initial pressure of800 kPa, each followed by a desorption reaction at the same temperature and a maximumbackpressure of 2 kPa. All samples present good stability, preserving at least 98% of theirinitial capacity after 100 cycles. Samples with composition LaNi5 and LaNi4.55Sn0.45 weresubjected to 1000 cycles, after which we observe a higher stability from the Sn-containingalloy (96% of the initial capacity preserved versus 92% for LaNi5). Absorption characteristictimes do not suffer important changes in either case. Desorption is gradually retardedwhen Sn content is higher than 0.4 at.","palabras_clave":"HIDRÓGENO|HIDRUROS|AB5|PURIFICACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1097,"idioma_id":4,"titulo":"Remote state preparation of a photonic quantum state via quantum tele- portation","resumen":"We demonstrate an experimental realization of remote state preparation via the quantum teleportation algorithm, using an entangled photon pair in the polarization degree of freedom as the quantum resource. The input state is encoded on the path of one of the photons from the pair. The improved experimental scheme allows us to control the preparation and teleportation of a state over the entire Bloch sphere with a resolution of the degree of mixture given by the coherence length of the photon pair. Both the preparation of the input state and the implementation of the quantum gates are performed in a pair of chained displaced Sagnac interferometers, which contribute to the overall robustness of the setup. An average fidelity above 0.9 is obtained for the remote state preparation process. This scheme allows for a prepared state to be transmitted on every repetition of the experiment, thus giving an intrinsic success probability of 1.","palabras_clave":"Quantum information processing|Teleportation|remote state preparation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1099,"idioma_id":5,"titulo":"Entre la confrontación y la funcionalidad. Poder Ejecutivo y Poder Judicial en torno a la política habitacional de la Ciudad de Buenos Aires","resumen":"El artículo analiza el proceso de movilización legal respecto de la política de subsidios habitacionales del Gobierno de la Cuidad de Buenos Aires. Lejos de representar casos de litigio estructural orientados a modificar la política pública, que son aquellos que captan la atención del mundo académico, este trabajo ilumina sobre un conjunto de casos de carácter individual, recurrente, rutinario y masivo que ocupan una gran parte del tiempo y de los recursos de las burocracias judiciales y del poder ejecutivo. Durante más de una década de litigio se establecieron circuitos políticos institucionales y prácticas adaptativas entre ambos poderes. Por un lado, la justicia local es accesible a las demandas individuales ofreciendo evidencia del acceso a los tribunales por parte de la población de bajos recursos; por otro lado, en sus interacciones con el poder ejecutivo, la justicia se consolida como ?la otra ventanilla? al facilitar la continuidad de los subsidio habitacionales para los esos sectores sin efectuar revisiones de la política que mejoren la calidad de vida de la población demandante de manera sustancial.","palabras_clave":"subsidios habitacionales|marginaciones sociales|judicialización|Ciudad de Buenos Aires"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1100,"idioma_id":5,"titulo":"Valoración de anticuerpos IgG asimétricos en suero sanguíneo y extractos placentarios porcinos","resumen":"La placenta porcina es epiteliocorial y no invasiva. El sistema inmune juega un papel durante la gestación que aún no se comprende. El objetivo del trabajo fue valorar el porcentaje de anticuerpos (Ac) IgG asimétricos en extractos placentarios y suero porcino de diferentes períodos de gestación. Se determinó la presencia de Ac IgG asimétricos por ELISA diferencial. Fueron procesados 45 muestras de suero sanguíneo, extractos placentarios maternos (HoPM) y fetales (HoPF), de cerdas gestantes de 30 (n=17), 65-70 (n=9) y 95- 114 (n:=6) d de gestación, así como 13 úteros de cerdas vacías (HoU). No se encontraron diferencias significativas en suero, entre cerdas vacías y cerdas gestantes en los porcentajes de Ac IgG asimétricos, (38±3 vs. 37±2, P&lt;0,74), aunque sí aparecen diferencias significativas entre cerdas de 30 y 95 d de gestación (32±3 vs 43±3, P&lt;0,01). Solamente a los 65-70 d de gestación se encontró anticuerpos asimétricos (45±2) en HoPM. Se hallaronvalores altos de Ac IgG asimétricos en HoPF durante toda la preñez, a los 30 d (52±5), a los 65-70 d (44±3) y a los 95-114 d (47±4). En conclusión, se postula que la presencia de Ac IgG asimétricos posibilita una preñez exitosa en porcinos.&nbsp;","palabras_clave":"INMUNOGLOBULINAS|PLACENTA|PORCINO|SUERO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1101,"idioma_id":4,"titulo":"Determination of the substitution degree of modified chitosan by cyclic voltammetry at the water\/dichloroethane interface","resumen":"The electrochemical behavior of the cationic polymer chitosan-gtmac (Ch-GTMAC), at the polarized water \/ 1,2-dichloroethane interface was studied. This polymer was obtained by nucleophilic reaction of chitosan with glycidyltrimethylammonium chloride groups (GTMAC). The voltammetric response was found to be dependent on the concentration of the polymer, on pH of the aqueous phase, and on the nature of the organic electrolyte. The results suggest a weak adsorption of the polymer coupled to the transfer towards the organic phase. Desorption process is quasi-reversible, and there is not interaction between adsorbents. From the analysis of desorption charge, it was possible to determine the substitution degree of the polymer.","palabras_clave":"Quitosan|voltametría cíclica|modificación química"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1102,"idioma_id":5,"titulo":"Pueraria montana var. lobata (Leguminosae) en la Argentina: estado actual de su naturalización","resumen":"En este trabajo se discuteel estado actual de naturalización de Pueraria montana (Lour.) Merr. var. lobata(Willd.) Maesen &amp; S.M. Almeida ex Sanjappa &amp; Predeep (Leguminosae,Papilionoideae) en la Argentina, considerada invasora en la provincia deMisiones y naturalizada en la de Buenos Aires, sobre la base de estudiosflorísticos y etnobotánicos. Además, incluye la descripción de la variedad,iconografía, distribución geográfica, biología reproductiva, nombresvernáculos, usos, mecanismos de expansión, observaciones y material dereferencia","palabras_clave":"Invasiones biológicas|especies exóticas|etnobotánica|Flora argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1103,"idioma_id":4,"titulo":"The use of electrical stimulation to guide epidural and intrathecal needle advancement at the L5 -L6 intervertebral space in dogs","resumen":"Objective To determine the minimal electrical threshold (MET) necessary to elicit appropriate muscle contraction when the tip of an insulated needle is positioned epidurally or intrathecally at the L5-6 intervertebral space (phase-I) and to determine whether the application of a fixed electrical current during its advancement could indicate needle entry into the intrathecal space (phase-II) in dogs.  Study design Prospective, blinded study.  Animals Thirteen (phase-I) and seventeen (phase- II) dogs, scheduled for a surgical procedure where L5-6 intrathecal administration was indicated.  Methods Under general anesthesia, an insulated needle was first inserted into the L5-6 epidural space and secondly into the intrathecal space and the MET necessary to obtain a muscular contraction of the pelvic limb or tail at each site was determined (phase- I). Under similar conditions, in dogs of phase-II an insulated needle was inserted through the L5-6 intervertebral space guided by the use of a fixed electrical current (0.8 mA) until muscular contrac- tion of the pelvic limb or tail was obtained. Intrathe- cal needle placement was confirmed by either free flow of cerebrospinal fluid (CSF) or myelography.  Results The current required to elicit a motor response was significantly lower (p < 0.0001) when the tip of the needle was in the intrathecal space (0.48 ` 0.10 mA) than when it was located epi- durally (2.56 ` 0.57). The use of a fixed electrical stimulation current of 0.8 mA resulted in correct prediction of intrathecal injection, corroborated by either free flow of CSF (n = 12) or iohexol distribu- tion pattern (n = 5), in 100% of the cases.  Conclusion and clinical relevance Nerve stimula- tion may be employed as a tool to distinguish epidural from intrathecal insulated needle position at the L5-6 intervertebral space in dogs. This study demonstrates the feasibility of using an electrical stimulation test to confirm intrathecal needle position in dogs.  Keywords dog, electrical threshold, epidural, intra- thecal, nerve stimulation test.","palabras_clave":"electrical threshold|epidural|intrathecal|nerve stimulation test"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1104,"idioma_id":4,"titulo":"Incentive relativity in middle aged rats","resumen":"Response to a reinforcer is affected by prior experience with different reward values of that reward, aphenomenon known as incentive relativity. Two different procedures to study this phenomenon are theincentive downshift (ID) and the consummatory anticipatory negative contrast (cANC), the former is anemotional-cognitive protocol and the latter cognitive one. Aged rodents, as also well described in agedhumans, exhibit alterations in cognitive functions. The main goal of this work was to evaluate the effectof age in the incentive? assessment using these two procedures. The results indicated that aged rats hadan adequate assessment of the rewards but their performance is not completely comparable to that ofyoung subjects. They recover faster from the ID and they had a cognitive impairment in the cANC. Theresults are discussed in relation to age-related changes in memory and emotion.","palabras_clave":"Age|Incentive relativity|memory|emotion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1108,"idioma_id":4,"titulo":"Identifying design and reduction effects on lithic projectile point shapes","resumen":"Since lithic tools are intended to accomplish certain functions as a response to environmental demands, their original design varies considerably from its very inception. Thus, exploring variability on the original designs can be informative of cultural adaptive processes on past populations. However, the complex life-cycle of a stone tool includes loops of damage due to use followed by breakage and resharpening that dramatically blur the size and shape attributes defining the original design. Here we the Factor Model, a statistical approach recently modified to be used in landmark data, to evaluate original design attributes versus changes attributed to maintenance activities on a sample of Southern Patagonia lithic stemmed points, including arrows and spears, likely throwing and thrusting ones. The model enables the separation of shape aspects that tend to covary because of common factors affecting simultaneously the two fundamental modules of a classical stemmed weapon (blade\/stem), from those shape features explained only by local factors affecting modules independently. Our results show that original design differences explain most of the total shape variation, and also indicate that maintenance patterns differ among point types considered as different weapon systems (arrows and spears). Whereas arrow reduction is focused on tip modifications, spears present a broader array of shape changes including the tip and the shoulders. These results demonstrate that disentangling the sophisticated interaction among original design and maintenance activities of lithic projectile points enables a proper and independent exploration of adaptation to functional demands and cognitive abilities of past populations.","palabras_clave":"LITHIC PROJECTILE POINTS|SHAPE VARIATIONS|GEOMETRIC MORPHOMETRICS|FACTOR MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1109,"idioma_id":4,"titulo":"Tree plantations on a grassland region: effects on methane uptake by soils","resumen":"We assessed the rates of methane  uptake (CH4 by soils of different tree plantations and a control to provide  information regarding potential greenhouse gas sequestration by tree  plantations of pines, mixed deciduous species and eucalyptus in a  natural grassland around 37 S, 59 W (Argentinean Pampa).  A  naturalized pasture was used as control.  All sites had been  agricultural and livestock lands. Each site was sampled three times  from December 2012 to May 2013, using 5 static chambers randomly  distributed across an area of ~100m2 in each site. In the control, methane fluxes were very weak; both  negative (uptake) and positive (emission) values were found. Below  tree plantations, fluxes were always negative, with statistically  significant intersite differences. The highest uptake rates were  observed in the mixed deciduous plantation (~10 ng m-2 s-1),  followed by pines and eucalyptus plantations. Intrasite differences  associated with spatial variation were also found. A  significant inverse correlation between CH4 uptake and  soil water content was found in the pine and deciduous species  plantations (R2 &gt;&gt;0.94, p&lt;0.1).","palabras_clave":"SOIL|TREE PLANTATION|METHANE UPTAKE|STATIC CHAMBER|LAND USES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1110,"idioma_id":4,"titulo":"On the sensitivity of extrasolar mass--loss rate ranges:  HD 209458b a case study","resumen":"We present a 3D hydrodynamic study of the effect that different stellar wind conditions  and planetary wind structures have on the calculated Ly&#945; absorptions produced by a cometary  tail during transit. We concentrate, as a case study, on the known HD 209458b case.  By comparing the numerically obtained Ly&#945; absorption with the observations, we find  that the planetary mass loss rate does not change dramatically for large changes in stellar   &#775; p range found  wind speeds &#8764;[250 &#8722; 800] km s&#8722;1 and temperature &#8764; [3 &#8722; 7] ×106 K. The M  is &#8764; [3 &#8722; 5] × 1010 g s&#8722;1 , depending upon the efficiency of the stellar wind to transport heat,  (polytropic index &#915; &#8764; [1.01 &#8722; 1.13]), leading to different stellar wind speeds. Several models  with anisotropic evaporation profiles for the planetary escaping atmosphere were carried out,  showing that both, the escape through polar regions, resembling the emission associated with  reconnection processes, and through the night side, produced by a strong stellar wind that  compresses the planetary atmosphere and inhibits its escape from the day h","palabras_clave":"EXOPLANETAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1111,"idioma_id":5,"titulo":"Hiatos de regeneración del bosque de Araucaria araucana en patagonia: vinculaciones al uso de tierras y desertificación regional","resumen":"  &amp;lt;!--   \/* Font Definitions *\/  @font-face  \t{font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';  \tmso-font-charset:78;  \tmso-generic-font-family:auto;  \tmso-font-pitch:variable;  \tmso-font-signature:1 134676480 16 0 131072 0;}  @font-face  \t{font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';  \tmso-font-charset:78;  \tmso-generic-font-family:auto;  \tmso-font-pitch:variable;  \tmso-font-signature:1 134676480 16 0 131072 0;}  @font-face  \t{font-family:Cambria;  \tpanose-1:2 4 5 3 5 4 6 3 2 4;  \tmso-font-charset:0;  \tmso-generic-font-family:auto;  \tmso-font-pitch:variable;  \tmso-font-signature:-536870145 1073743103 0 0 415 0;}   \/* Style Definitions *\/  p.MsoNormal, li.MsoNormal, div.MsoNormal  \t{mso-style-unhide:no;  \tmso-style-qformat:yes;  \tmso-style-parent:'';  \tmargin:0cm;  \tmargin-bottom:.0001pt;  \tmso-pagination:widow-orphan;  \tfont-size:12.0pt;  \tfont-family:Cambria;  \tmso-ascii-font-family:Cambria;  \tmso-ascii-theme-font:minor-latin;  \tmso-fareast-font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';  \tmso-fareast-theme-font:minor-fareast;  \tmso-hansi-font-family:Cambria;  \tmso-hansi-theme-font:minor-latin;  \tmso-bidi-font-family:'Times New Roman';  \tmso-bidi-theme-font:minor-bidi;}  .MsoChpDefault  \t{mso-style-type:export-only;  \tmso-default-props:yes;  \tfont-family:Cambria;  \tmso-ascii-font-family:Cambria;  \tmso-ascii-theme-font:minor-latin;  \tmso-fareast-font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';  \tmso-fareast-theme-font:minor-fareast;  \tmso-hansi-font-family:Cambria;  \tmso-hansi-theme-font:minor-latin;  \tmso-bidi-font-family:'Times New Roman';  \tmso-bidi-theme-font:minor-bidi;}  @page WordSection1  \t{size:595.0pt 842.0pt;  \tmargin:70.85pt 3.0cm 70.85pt 3.0cm;  \tmso-header-margin:35.4pt;  \tmso-footer-margin:35.4pt;  \tmso-paper-source:0;}  div.WordSection1  \t{page:WordSection1;}  --&amp;gt;      Los bosques de  Araucaria araucana (pehuén) son influenciados en su dinámica de regeneración por  elementos naturales o antrópicos. Cambios climáticos recientes contribuir.an a  declinaciones del crecimiento y probablemente en la intensidad de regeneración.  Sin embargo, aún luego de extraer madera, vastas extensiones con fisonomía de  parque resultaron afectados por acción del fuego en combinación con la  sobrecarga de ganado introducido desde fines del siglo XIX.    Este ganado,  condicionado a reducidos corredores de trashumancia y .reas de pastaje, impacta  sobre la regeneración del bosque de pehuén en el noroeste de Patagonia  argentina. El ganado trashumante, desplazándose desde campos bajos y áridos de  invernada a los más húmedos y de mayor receptividad durante el verano en valles  cordilleranos, consume retoños y plantas recién germinadas de pehuén, mientras  que regresando a campos de invernada consumen la semilla.    Como consecuencia,  sectores de bosque de pehuén se encuentran escasamente representados respecto  de clases etarias menores a 100 años. En este trabajo revisamos los  antecedentes relativos a esta ruptura en la estructura etaria que conduce al  envejecimiento de las poblaciones de pehuén. Los factores de disturbio  mencionados en sociedad con la sobrecarga animal, se revelan como importantes  factores de desertificación en territorios del noroeste patagónico.","palabras_clave":"REGENERACION FORESTAL|DESERTIFICACION|PATAGONIA|PEHUEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1112,"idioma_id":5,"titulo":"Redes para la promoción del desarrollo territorial en el cinturón hortícola platense Reflexiones y aportes","resumen":"En el cinturón hortícola platense, un conjunto de instituciones y organizaciones del sector público y privado  establecen vínculos entre ellas y con el sector productivo con distintos fines, y así conforman redes. El trabajo  tiene como objetivo conocer y analizar las redes sociotécnicas que contribuyen al desarrollo territorial. Se utilizó  una metodología de tipo cualitativa, con entrevistas semiestructuradas a técnicos del sector público y privado, y a  representantes del sector productivo. Los resultados muestran una diversidad de redes sociotécnicas, en las que  participan distintos actores, y en las que se promueve un conjunto de innovaciones de distinto tipo.","palabras_clave":"cinturón hortícola|redes sociotécnicas|público-privado|actores"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1114,"idioma_id":5,"titulo":"Prevalencia de anemia y deficiencia de hierro, ácido fólico y vitamina B12 en una comunidad wichí del noroeste argentino","resumen":"INTRODUCCIÓN: La anemia y malnutrición son prevalentes en comunidades de bajo nivel socioeconómico y con higiene inadecuada, como las aborígenes Wichí que habitan en el Noroeste Argentino, región con alta prevalencia de enfermedad de Chagas.   OBJETIVO: Determinar la prevalencia de anemia y deficiencia de hierro (Fe), ácido fólico (AF) y vitamina B12 (B12) en una comunidad wichí de Salta; y evaluar la presencia de Chagas y talasemias como etiología de anemia.  MATERIAL Y MÉTODOS: Se realizó un estudio observacional descriptivo en mayo de 2008. Se estudiaron 35 adultos wichí (21 mujeres, 14 hombres) de la comunidad La Unión. El grupo control consistió en 36 criollos adultos (21 mujeres, 15 hombres). Se realizó hemograma, Fe, transferrina, ferritina, AF, B12, electroforesis de hemoglobina (EHb) y serología para Chagas.  RESULTADOS: La prevalencia de anemia fue 76% (16\/21; Intervalo de Confianza [IC] 95%= 57-95%) en las mujeres wichí; en las criollas, 29% (6\/21; IC95%= 10-48%); en el grupo masculino criollo, 7% (1\/15; IC95%= 0-20%) y en los wichís, 29% (4\/14; IC95%= 16-42%). En las mujeres wichí, el 75% (12\/16) de los casos fueron por deficiencia de Fe. B12 y EHb fueron normales. El 15% (5\/33) del grupo wichí y el 31% (11\/35) del criollo presentó deficiencia de AF. La prevalencia de Chagas fue 31% (11\/35; IC95%= 16-46%) en wichís y 28% (10\/36; IC95%= 13-43%) en criollos, y no estuvo asociada a anemia.  CONCLUSIONES: La alta prevalencia de anemia y deficiencia de hierro indican un problema de salud y de nutrición importante en esta comunidad.","palabras_clave":"ANEMIA|ORGANIZACIONES INDÍGENAS|DEFICIENCIA DE HIERRO|ÁCIDO FÓLICO|VITAMINA B12"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1115,"idioma_id":4,"titulo":"Manufacturing and Testing of a Sandwich Panel Honeycomb Core Reinforced with Natural-fiber Fabrics","resumen":"A novel honeycomb core made of a natural-fiber reinforced composite consisting of a vinylester matrix reinforced with jute fabric is introduced. Six-mm- and 10-mm-cell honeycombs are manufactured using two compression-molding techniques. Best results are obtained for the mold with lateral compression. Experimental tests are conducted to characterize the elastic response of the composite and the core   response under flatwise compression. The effective elastic properties of the core are computed via a homogenization analysis and finite element modeling. The results of the homogenization analysis are in very good agreement with estimations done using analytical formulas from the bibliography. The flatwise compression tests show that the core failure mechanisms are yarn pull-out and fiber breaking. The large wall thickness relative to the cell size of the jute-vinylester cores, which inhibits buckling, and the heterogeneities in the composite, which are referential   damage initiation sites, explain the observed behavior. When compared in terms of the specific strengths, the jute\/vinylester cores introduced in this work show similar performances to those of their commercially available counterparts. The results from this study suggest that jute-reinforced cores have the potential to be an alternative   to standard cores in applications that sustain compressive static loads.","palabras_clave":"Honeycomb cores|Natural Fibres|Mechanical Characterization|Effective Elastic Properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1116,"idioma_id":4,"titulo":"A new combined quasigeoid model in Tierra del Fuego","resumen":"ABSTRACT    Since 1998, several scientific institutions have contributed to collect gravity data and to establish levelling lines, including accurate GPS coordinates, on the island of Tierra del Fuego, Argentina.  Once these measurements were carried out, two geoid models were developed. One was purely gravimetric (Font, 2001) and the other was a geometric model (Del Cogliano, 2001).   This work focuses on the development of a combined quasigeoid model for this area. The equivalent source technique (EST) was applied to combine different kind of data. In this work gravity and GPS\/levelling were merged.  New global geopotential models (GGM) were introduced in the discussion, particularly the EGM2008 (Pavlis et al., 2008), which was used for evaluation purposes.   After a cross validation procedure a quasigeoid model accurate to 5 cm (r.m.s.) was finally achieved for the major part of the island.     KEYWORDS: Tierra del Fuego, quasigeoid, GPS\/levelling, gravity, Equivalent Source Technique","palabras_clave":"TIERRA DEL FUEGO|QUASIGEOID|GPS\/LEVELLING|GRAVITY|EQUIVALENT SOURCE TECHNIQUE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1119,"idioma_id":5,"titulo":"Intervención diferencial de las funciones ejecutivas en inferencias elaborativas y puente","resumen":"En la actualidad se considera la comprensión lectoracomo un proceso constructivo que depende de la participaciónde componentes para crear una representaciónen la mente del lector. La construcción de este modelomental requiere de la elaboración de inferencias puentey elaborativas. El objetivo del trabajo fue evaluar el roldiferencial de los procesos controlados y conscientesen la ejecución de ambos tipos de inferencias. Se evaluaron107 niños de 8 y 9 años, alumnos de escuelas degestión pública y privada de Mar del Plata, Argentina.Se utilizaron siete tareas de evaluación de las FuncionesEjecutivas y dos tareas de realización de inferencias.Los resultados mostraron una asociación fuerte entre elfuncionamiento ejecutivo y la generación de inferenciaselaborativas.","palabras_clave":"INFERENCIAS |FUNCIONES EJECUTIVAS|COMPRENSION|INFANCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1120,"idioma_id":4,"titulo":"Effects of live-shearing on population parameters and movement in sedentary and migratory populations of guanacos Lama guanicoe","resumen":"Live-shearing of wild guanacos Lama guanicoe may affect their reproductive success and population resilience, and therefore it is important to assess the  biological sustainability of obtaining their wool. We evaluated effects of capture and shearing on survival and reproduction, population parameters, daily movements, ranging behaviour and spatial distribution in sedentary and migratory populations. We assessed population variables by radio-telemetry and line-transect surveys before and after capture. We estimated high post-shearing survival rates in both populations and similar yearling production in shorn and non-shorn females in the migratory population. We did not find significant variations in density and population structure before and after shearing in the sedentary population, whereas in the migratory population density decreased and the population structure changed significantly after assembly of the capture structure, returning to pre-assembly levels 1 month later. The mean daily  distance moved by radio-collared guanacos during the first 2 days after shearing was three times longer than during the following 30 days. There was a 25% decrease in the mean home-range size of shorn guanacos between the first and  second month after shearing but this decline appeared to be associated with a seasonal change in movement, because a similar reduction occurred during the same period the following year, when the guanacos were not shorn. Live-shearing modified the spatial distribution pattern in the sedentary population but did not have a significant effect on the migratory population.Management of guanacos may contribute towards developing a biologically sustainable economic activity that promotes conservation of wildlife and habitats.","palabras_clave":"ARID ECOSYSTEM|BIODIVERSITY CONSERVATION|LAMA GUANICOE|POPULATION PARAMETERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1121,"idioma_id":5,"titulo":"Cáncer y su asociación con patrones alimentarios en Córdoba (Argentina)","resumen":"Introducción: La alimentación es un importante factor vinculado a la ocurrencia del  cáncer. Su abordaje en términos de patrones alimentarios es de creciente  interés en epidemiología nutricional, no obstante ha sido pocas veces empleado  en Latinoamérica.  Objetivos: Identificar patrones alimentarios en la población adulta de Córdoba  (Argentina) y estimar sus efectos sobre el riesgo de ocurrencia de cáncer  colorrectal (CCR), cáncer de mama (CM), cáncer de próstata (CP) y urotelial  (CU). Métodos: Se condujeron estudios caso-control, 2006-2012, correspondientes a CCR,  CM, CP y CU. Empleando un análisis factorial de componentes principales se  identificaron patrones alimentarios. Se estimaron ORs mediante regresión  logística multinivel. Resultados: Se identificaron patrones característicos en la  población general, y en hombres y mujeres independientemente. En población  total, los patrones Cono Sur y Bebidas Azucaradas evidenciaron un efecto  promotor para CCR y CU, y el Patrón Prudente mostró efecto protector. En  mujeres, el CM se asoció de manera directa con los patrones Cono Sur Femenino, Rural y Amiláceo, e  inversa con el Patrón Prudente. En  hombres, los Patrones Cono Sur Masculino,  Bebidas Azucaradas y Típico Mesurado mostraron un efecto  promotor para CP. Conclusión: Resulta necesario promover una ingesta habitual de vegetales, frutas y  lácteos (éstos últimos de manera moderada en hombres), y disminuir el consumo  de carnes rojas (fundamentalmente grasas), carnes procesadas, vegetales  amiláceos, vino y bebidas azucaradas, a fin de prevenir la ocurrencia de  cáncer. En hombres se sugiere moderar la ingesta de huevos, y en mujeres la de  granos refinados, productos de pastelería, aceites y mayonesa.","palabras_clave":"PATRONES ALIMENTARIOS|CASO CONTROL|CANCER|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1122,"idioma_id":4,"titulo":"Root  proliferation  in  native  perennial  grasses  of  arid Patagonia, Argentina","resumen":"Pappophorum vaginatum is the most abundant C4 perennial grass desirable to livestock in rangelands of northeastern Patagonia, Argentina. We hypothesized that (1) defoliation reduce net primary productivity, and root length density and weight in the native species, and (2) root net primary productivity, and root length density and weight, are greater in P. vaginatum than on the other, less desirable, native species (i.e., Aristida spegazzinii, A. subulata and Sporobolus cryptandrus). Plants of all species were either exposed or not to a severe defoliation twice a year during two growing seasons. Root proliferation was measured using the cylinder method. Cylindrical, iron structures,  wrapped up using nylon mesh, were buried diagonally from the periphery to the center on individual plants. These structures, initially filled with soil without any organic residue, were dug up  from the soil on 25 April 2008, after two successive defoliations in mid-spring 2007. During the second growing season (2008\/2009), cylinders were destructively harvested on 4 April 2009, after one or two defoliations in mid- and\/or late-spring, respectively. Roots grown into the cylinders were obtained after washing the soil manually. Defoliation during two successive years did reduce the study variables only after plants of all species were defoliated twice, which supported the first hypothesis. The greater root net primary productivity, and root length density and weight in P. vaginatum than in the other native species, in support of the second hypothesis, could help to explain its greater abundance in rangelands of Argentina","palabras_clave":"Aristida species|Competitive ability|Pappophorum vaginatum|Sporobolus cryptandrus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1123,"idioma_id":5,"titulo":"'Aplicaciones de caolin para el control del golpe de sol en mandarino Okitsu'.","resumen":"En mandarinos cultivados en regiones con alta insolación es frecuente observar daños producidos por sol, principalmente en frutos ubicados en la periferia. El objetivo de este trabajo fue evaluar el efecto de la aplicación de partículas de caolín, en diferentes dosis y momentos, para el control del golpe de sol en Citrus unshiu Marc. cv. ´Okitsu´. Se probaron siete tratamientos con caolín 95 %, combinaciones de dosis (2 y 4 %), aplicadas con diferentes frecuencias (dos, tres y cuatro aplicaciones), en un diseño experimental de bloques completos al azar con cuatro repeticiones y tres plantas por réplica. Antes de la cosecha se muestrearon 15 frutos por parcela experimental, en los que se determinó diámetro (mm), jugo (%), sólidos solubles totales (°Brix), acidez total titulable (mL) e índice de madurez (ratios). Se seleccionaron al azar 40 frutos por parcela y se clasificaron según tres niveles de daño por sol: frutos sin daño (G.0); frutos con manchas de color amarillo de hasta un 15 porciento de la corteza (G.1); frutos con manchas de color amarillo en más de 16 porciento de la corteza y tejido necrosado oscuro (G.2). Los tratamientos con aplicación presentaron entre 91,53 y 97,94 % de frutos G.0, mientras que el testigo solamente alcanzó el 73,97 %. La aplicación de caolín resultó efectiva para el control de daño por sol en mandarino ´Okitsu´. Cuatro aplicaciones al 2 % o tres aplicaciones al 4 %, de caolín 95 %, permiten lograr 97 % de frutos libres de daño, sin afectar la calidad interna.","palabras_clave":"CITRICULTURA|QUEMADURA|ESCALDADURA|DOSIS DE APLICACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1124,"idioma_id":4,"titulo":"Cobalt Supported on Zirconia. Selective Catalytic Reduction of NO Using Propane as Reducing Agent","resumen":"The catalytic behavior of a series of cobalt catalysts supported on zirconia in the selective catalytic reduction(SCR) of NO has been studied using propane as reducing agent. The catalysts were characterized by Raman Spectroscopy, Temperature Programmed Reduction (TPR), Diffuse Reflectance Spectroscopy UV-Vis (DRS) and X-ray Photoelectron Spectroscopy (XPS) to assess the physicochemical properties of cobalt species. Results for SCR of NO indicate that the activity is substantially related to the cobalt concentration and consequent nature of supported cobalt species. The formation of segregated oxidic phases on the catalytic surface produces an activity decrease. The most active catalyst contains Co(II) ions, interacting with the support, in tetrahedral coordination.","palabras_clave":"CO\/ZIRCONIA|SURFACE CHARACTERIZATION|SCR|NOX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1126,"idioma_id":4,"titulo":"Differences in natural antibody titres comparing free-ranging guanacos (Lama guanicoe) and capybaras (Hydrochoerus hydrochaeris)","resumen":"Natural antibodies are an important component of innate humoral immunity, but have not been investigated in wild mammals to any great extent. In the current study, serum natural antibody titres were measured by hemagglutination assay for two South American herbivores, the guanaco (Lama guanicoe) and the capybara (Hydrochoerus hydrochaeris). Results indicated  29 that capybaras had antibody titres on average more than 4 times higher than guanacos (median titres 1:256 and 1:4, respectively), suggesting evolutionary differences in host immunological responses between the two species.","palabras_clave":"natural antibodies|anticuerpos naturales|guanacos|carpinchos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1127,"idioma_id":4,"titulo":"Fumonisin occurrence in naturally contaminated wheat grain harvested in Argentina","resumen":"A survey was carried out to determine fumonisin contamination in 135 common and 40 durum wheat samples collected during 2011 harvest season (non-FHB epidemic year) in the main wheat production area of Argentina using LCeMS\/MS. A 93% of total samples showed fumonisin contamination, with levels ranging from 0.16 to 680.44 ng\/g in common and from 0.15 to 1304.39 ng\/g in durum wheat samples,respectively. FB1 was the fumonisin most frequently found during the evaluated year. Twenty five wheat samples (15 common and 10 durum) were selected for a deoxynivalenol (DON) analysis among all the samples analyzed for fumonisin content using different contamination levels as selection criteria. DON contamination was present in 24 out of 25 wheat samples, the levels ranging from 50.60 to 28650 ng\/g. Nine out of 25 wheat samples reached values higher than 1000 ng\/g. However there was no correlation between fumonisin and DON contamination. This is the first report of natural fumonisin presence in common wheat grains in Argentina, as well as of DON co-occurrence in both types of wheat.","palabras_clave":"FUMONISIN|DURUM WHEAT|COMMON WHEAT|DEOXYNIVALENOL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1128,"idioma_id":4,"titulo":"Low-fat meat sausages with fish oil: Optimization of milk proteins and carrageenan contents using response surface methodology","resumen":"Response surface methodology was used to analyze the effect of milk proteins and 2:1 &#954;:&#953;-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of lowfatmeat sausages with pre-emulsified fish oil. A central-composite design was used to develop modelsfor the objective responses. Changes in carrageenans affected more the responses than milk proteinslevels. Convenience functions were calculated for CL, WLC, hardness, and springiness of the product.Responses were optimized simultaneously minimizing CL and WLC; ranges for hardness andspringiness corresponded to commercial products (20 g of pork fat \/100g). The optimumcorresponded to 0.593 g of carrageenans \/100g and 0.320 g of milk proteins and its total lipid contentwas 6.3 g\/100g. This formulation was prepared and evaluated showing a good agreement betweenpredicted and experimental responses. These additives could produce low-fat meat sausages with preemulsifiedfish oil with good nutritional quality and similar characteristics than traditional ones.","palabras_clave":"LOW FAT MEAT SAUSAGES|FISH OIL|TEXTURE|RESPONSE SURFACE METHODOLOGY|OPTIMIZATION|WATER BINDING PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1132,"idioma_id":5,"titulo":"Franjas trampa de Brassica campestris L. en cultivo de repollo. Efecto sobre pulgones y sus parasitoides","resumen":"El  manejo de la diversidad vegetal en los agrosistemas afecta la dinámica  poblacional de las plagas y de sus enemigos naturales, siendo una alternativa  de control el uso de franjas trampas. El objetivo fue determinar el efecto de franjas trampas  marginales de Brassicas campestris,  en el ataque de pulgones de B. oleracea var. capitata  y sus  parasitoids. El trabajo se realizó en el cinturón hortícola santafesino  desde febrero de 2012, implantándose un lote comercial de repollo en surcos (28.000  plantas\/ha) y, en el borde norte la franja trampa. En el cultivo se  establecieron cuatro tratamientos de 17 m x 7 m: 1) con franja marginal, sin  agroquímicos; 2) con franja marginal, con agroquímicos; 3) sin franja marginal,  sin agroquímicos, 4) sin franja marginal, con agroquímicos. Semanalmente, en  tres plantas, desde el trasplante hasta la cosecha, se contaron áfidos sanos y  parasitoidizados, determinándose el efecto de tres distancias de la franja  sobre cultivo. Estadísticamente se analizó mediante ANOVA comparándose las  medias con Tukey (&#945;&#8804;0,05). Se hallaron diferencias significativas en la  población de áfidos entre las franjas trampa y el cultivo. En el repollo los  recuentos de ninfas fueron mayores en el tratamiento con franja marginal, sin  aplicaciones de agroquímicos. Las franjas marginales y las distancias a ella no  influyeron en los parasitoides.","palabras_clave":"biodiversidad|Brevicoryne brassicae|Mizus persicae|Diaeretiella rapae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1133,"idioma_id":4,"titulo":"The explosive pollination mechanism in Papilionoideae (Leguminosae): an analysis with three Desmodium species","resumen":"In the papilionaceous flowers with explosive pollination mechanism, the androecium and the gynoecium are subjected to great pressure in the keel. It has been suggested that at the first visit nearly all pollen grains are released and that the floral parts do not return to their original positions; this would be a cue for pollinators to avoid activated flowers. The dependence on the pollinators suggests that explosive flowers will not produce fruits if themechanism is not activated. In this paper, we have tested these ideas studying the reproductive biology of three Desmodium species, D. incanum, D. subsericium and D. uncinatum. Specifically, we studied the floral traits, reproductive system, pollination mechanism and floral visitors, for the three species. The results indicated that the keel and the wings play a key role in the activation of the explosive mechanism. The three species have a secondarypollen presentation, diurnal flowers, and a short floral cycle. According to the results, all the species studied were self-compatible, with two species producing fruits by autonomous self-pollination. Desmodium flowers were visited and activated by native and exotic bee species. During the first visit nearly all the pollen was released; after that, new visits were low or null. Our results indicated that the activation of the mechanism is not essential for theproduction of fruits, that explosive flowers are visited only once and that the changes in the positions of the floral parts are the cues that keep pollinators away from the flowers with activated mechanism.","palabras_clave":"DESMODIUM|EXPLOSIVE POLLINATION|KEEL BLOSSOM|PAPILIONOIDEAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1134,"idioma_id":4,"titulo":"Some advances on the set covering polyhedron of circulant matrices","resumen":"Studying the set covering polyhedron of consecutive ones circulant matrices, Argiroffo and Bianchi found a class of facet defining inequalities, induced by a particular family of circulant minors. In this work we extend these results to inequalities associated with every circulant minor. We also obtain polynomial separation algorithms for particular classes of such inequalities.","palabras_clave":"CIRCULANT MATRIX|SET COVERING POLYHEDRON|SEPARATION ROUTINES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1136,"idioma_id":4,"titulo":"Characterization of Steady-State Current Ripple in  Interleaved Power Converters Under Inductance  Mismatches","resumen":"Interleaved power converters are used in highcurrent applications due to their inherent reduction of semiconductors stress and total ripple. Ripple reduction is accomplished by a correct phase-shifting, and the filtering improvement explained by the increase in ripple frequency. However, these benefits are wasted, among other reasons, by the mismatch  of the phase inductor value. As a consequence, differences in ripple amplitude among phases are produced, leading to a total current ripple significantly greater than the ideal case, the loss of the cancelation points and the generation of the switching frequency component and its harmonics. The works dealing with this subject matter have focused on particular cases, such as a given number of phases, a specific converter topology or a particular case of inductance mismatch, disregarding a general analytical approach. This work proposes an analytical method  to characterize the total ripple in steady-state as a function of the duty cycle and the number of phases under any condition on inductance mismatch. Experimental results validate the proposed method.","palabras_clave":"INTERLEAVED POWER CONVERTERS|CURRENT RIPPLE|INDUCTANCE TOLERANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1137,"idioma_id":4,"titulo":"Distorted wave calculations for electron loss process induced by bare ion impact on biological targets","resumen":"Distorted wave models are employed to investigate the electron loss process induced by bare ions on biological targets. The two main reactions which contribute to this process, namely, the single electron ionization as well as the single electron capture are here studied. In order to further assess the validity of the theoretical descriptions used, the influence of particular mechanisms are studied, like dynamic screening for the case of electron ionization and energy deposition on the target by the impacting projectile for the electron capture one. Results are compared with existing experimental data. ","palabras_clave":"IONIZATION|IONS|MOLECULES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1139,"idioma_id":4,"titulo":"Accelerator-Based BNCT","resumen":"The activityinacceleratordevelopmentforaccelerator-basedBNCT(AB-BNCT)bothworldwideandin  Argentina isdescribed.ProjectsinRussia,UK,Italy,Japan,Israel,andArgentinatodevelopAB-BNCT  around differenttypesofacceleratorsarebriefly presented.Inparticular,thepresentstatusandrecent  progressoftheArgentineprojectwillbereviewed.Thetopicswillcover:intenseionsources,accelerator  tubes, transportofintensebeams,beamdiagnostics,the 9Be(d,n) reactionasapossibleneutronsource,  Beam ShapingAssemblies(BSA),atreatmentroom,andtreatmentplanninginrealisticcases.","palabras_clave":"Accelerator-based BNCT |Electrostatic accelerators |Ion sources"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1140,"idioma_id":5,"titulo":"La agricultura familiar en las políticas de desarrollo rural, ¿hacia una nueva agenda pública? La experiencia reciente en Argentina (1990-2011","resumen":"RESUMEN: Las cuestiones vinculadas con el desarrollo rural y, particularmente, la agricultura familiar han mostrado diferentes movimientos en relación con su ingreso y tratamiento en la agenda pública en Argentina. Una situación similar ocurre con la consideración del 'agricultor familiar' como sujeto de política pública, que ha variado de acuerdo a contextos diferenciados. Considerando lo anterior, el propósito de este escrito es describir los procesos de jerarquización institucional y política de la agricultura familiar -en tanto sujeto central del desarrollo rural- a partir de la década de 1990 y hasta 2011 en la experiencia argentina. Asimismo, se presentarán las acciones tendientes a discutir y consensuar una definición operativa para la elaboración de políticas públicas para el sector. El estudio presenta un encuadre metodológico de tipo cualitativo con un diseño flexible. En este sentido, se analizaron y sistematizaron diversas fuentes documentales oficiales y bibliográficas especializadas sobre la temática e información primaria proveniente de los censos agropecuarios en Argentina.&nbsp; ","palabras_clave":"AGRICULTURA FAMILIAR|DESARROLLO RURAL|AGENDA PÚBLICA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1142,"idioma_id":4,"titulo":"Pressure and microwave sensors\/actuators based on smart hydrogel\/conductive polymer nanocomposite","resumen":"A nanocomposite is fabricated by formation of a conductive polymer, using in situ oxidative polymerization, inside a thermosensitive crosslinked hydrogel. FE-SEM micrographs show the nanometric domains of the conductive material (polyaniline, PANI) dispersed in the hydrogel matrix based on cross linked poly(N-isopropylacrylamide) (PNIPAm). The thermosensitive properties of PNIPAm and copolymers with 2-acrylamido-2-methyl propane sulfonic acid (AMPS) are not affected by the presence of conductive polymer nanoparticles. The incorporation of PANI improves the mechanical properties of the hydrogel allowing it to swell up to 30,000% without breaking. Since the conductive polymer absorbs strongly microwave radiation at pH  4, PANI is not conductive and the nanocomposite becomes insensitive to microwaves. However, using a pH insensitive conductive polymer (polypyrrole, PPy) inthe nanocomposite makes it sensitive to microwaves at all pH values. The nanocomposite is used in a chemomechanical actuator where drug release is driven remotely by microwave irradiation. Since the PNIPAm-co-2%AMPS\/PANI nanocomposite is soft and electronically conductive, could be used as pressure\/force sensor. It is shown that a compressive force applied on a cylinder of that nanocomposite increases the conductivity of material. Additionally a switch is built which turns off upon microwave irradiation. Therefore, the nanocomposites are potential candidates for different technological applications, such as: a force\/pressure electrical sensor, a drug delivery device driven remotely by microwaves, pH or temperature electrical switches and an electric switch driven by microwaves.","palabras_clave":"sensors|actuator|polyaniline |HYDROGELS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1144,"idioma_id":4,"titulo":"Hsp70 REGULATION ON Nox4\/p22phox AND CYTOSKELETAL INTEGRITY AS AN EFFECT OF LOSARTAN IN VASCULAR SMOOTH MUSCLE CELLS","resumen":"A series of signaling cascades are activated after angiotensin II binds to angiotensin II type I receptor (AT1R), a peptide that is an important mediator of oxidative stress. Hsp70 regulates a diverse set of signaling pathways through interactions with proteins. Here, we tested the hypothesis of angiotensin II AT1R inhibition effect on Hsp70 interaction with Nox4\/p22phox complex and Hsp70 leading to actin cytoskeleton modulation in spontaneously hypertensive rats (SHR) vascular smooth muscle cells (VSMCs). SHR and Wistar-Kyotto rats (VSMCs from 8 to 10 weeks) were stimulated with angiotensin II (100 nmol\/L) for 15 min (AII), treated with losartan (100 nmol\/L) for 90 min (L), and with losartan for 90 min plus angiotensin in the last 15 min (L&#8201;+&#8201;AII). Whereas SHR VSMCs exposure to angiotensin II overexpressed AT1R and Nox4 nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase and slightly downregulated caveolin-1 expression, losartan decreased AT1R protein levels and increased caveolin-1 and Hsp70 expression in SHR VSMC membranes. Immunoprecipitation and immunofluorescence confocal microscopy proved interaction and colocalization of membrane translocated Hsp70 and Nox4\/p22phox. Increased levels of Hsp70 contrast with the decreased immunoprecipitation of Nox4\/p22phox and RhoA in membranes from SHR VSMCs (L) vs SHR VSMCs (AII). Hsp72 depletion resulted in higher Nox4 expression and increased NADPH oxidase activity in VSMCs (L&#8201;+&#8201;AII) from SHR when contrasted with nontransfected VSMCs (L&#8201;+&#8201;AII). After Hsp72 knockdown in SHR VSMCs, losartan could not impair angiotensin II-enhanced stress fiber formation and focal adhesion assembly. In conclusion, our data showing a negative regulation of Hsp70 on Nox4\/p22phox demonstrates a possible mechanism in explaining the antioxidative function joined to cytoskeletal integrity modulation within the effects of losartan in VSMCs from SHR.","palabras_clave":"Oxidative stress|Heat Shock Proteins|NADPH subunits|Spontaneously Hypertensive Rats"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1145,"idioma_id":4,"titulo":"PHYTOPLANKTON DEVELOPMENT IN A HIGHLY EUTROPHIC MAN-MADE LAKE FROM THE PAMPA PLAIN OF ARGENTINA. A FUNCTIONAL APPROACH","resumen":"The phytoplankton structure and dynamics were analysed in relation to abiotic variables in Paso de las Piedras Reservoir, Argentina. Phytoplankton driving forces were explored using a functional approach. A total of 15 functional groups were identified among which D, P, H1, F, J and C were the most important. A Canonical variate analysis indicated that the categorization of the species in functional groups and its relationship with the temporal species clustering was not a random result. In the redundancy analysis, temporal ordination dynamics of functional groups was well described by the distribution of samples. Remarkable shifts in composition and biomass of phytoplankton occurred in agreement with changes in temperature, N:P ratio and transparency. The group succession could be summarized as: P\/J (early summer)&#8594;P\/ H1 (late summer)&#8594;P (early autumn)&#8594;P\/F (late autumn)&#8594;C (early winter)&#8594;D (late winter)&#8594;Y (early spring)&#8594;D (early spring)&#8594;P (late spring). The majority of the associations detected were typical of eutrophichypertrophic systems. With the exception of H1, the groups that most contribute to biovolume were those characteristics of mixed aquatic environments, i. e., non-stratifying lakes or deep, well-mixed epilimnia of stratifying lakes. The diversity indices, in terms of functional groups, were low, meaning that a small number of traits were present and\/or that there was an inequitable distribution","palabras_clave":"FUNCTIONAL GROUPS|PHYTOPLANKTON DYNAMICS|PASO DE LAS PIEDRAS RESERVOIR |DIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1147,"idioma_id":4,"titulo":"A Non-Linear Approach for Assessing Stability in Line-Commutated Converters with an Integrating Controller","resumen":"Closed-loop stability analysis of Line-Commutated Converters (LCCs) has been mainly based on linear, small-signal plant transfer modeling for the power stage. The inaccuracies derived from the use of these simple models in the frequency domain have led to conservative compensation strategies with reduced loop bandwidths. This article presents a non-linear time-domain approach for modeling LCCs under integrating control. The proposed technique was found useful to assess the onset of loop instability under different operating conditions. Bifurcations and possible routes to chaos in the parameter domain are also explored. The method is validated by simulation, and stability boundary conditions leading to period-doubling behavior are demonstrated by means of experimental results.","palabras_clave":"CONTROLLED-RECTIFIERS|LINE-COMMUTATED CONVERTERS|CHAOTIC MAPPING|BIFURCATIONS|STABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1148,"idioma_id":4,"titulo":"A two-scale failure model for heterogeneous materials: numerical implementation based on the finite element method","resumen":"In a first part of this contribution, a compact theoretical revision of the mechanical and variational foundations of a Failure-Oriented Multi-scale Formulation (FOMF) devised for modeling failure in heterogeneous materials is described.The proposed model considers two well separated physical length scales, namely: (i) the macro scale where nucleation and evolution of a cohesive surface is considered as a medium to characterize the phenomenon taking place at the lower scale of length, and (ii) The micro scale where some mechanical processes that leads to the material failure are taking place, such as strain localization, damage, shear band formation, etc. These processes are modeled using the concept of Representative Volume Element (RVE).In the macro scale, the traction separation response, characterizing the mechanical behavior of the cohesive interface, is a result of the simulated failure processes taking place in the micro scale, and it is obtained by a particular homogenization technique applied on specific RVE sub-domains. Standard, as well as, Non-Standard boundary conditions are consistently derived in order to preserve ?objectivity? of the homogenized response with respect to the micro-cell size.In a second part of the paper, and as an original contribution, the detailed numerical implementation of the two-scale model based on the Finite Element Method is presented. Special attention is devoted to the topics which are distinctive of the FOMF, such as: (i) the finite element technologies adopted in each scale along with their corresponding algorithmic expressions, (ii) the generalized treatment given to the kinematical boundary conditions in the RVE and (iii) how these kinematical restrictions affect the capturing of macroscopic material instability modes and the posterior evolution of failure at the RVE level.Finally, a set of numerical assessments is performed in order to show the potentialities of the proposed methodology, as well as, to compare and verify the numerical solution provided by the two-scale model with respect to a mono-scale Direct Numerical Simulation (DNS) approach","palabras_clave":"Multi-scale formulations| Computational homogenization|Representative Volume Element (RVE)|Failure modeling of heterogeneous materials"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1149,"idioma_id":5,"titulo":"Satisfactores sociales en periferias urbanas internas y externas","resumen":"En este artículo se  exponen los fundamentos teóricos y metodológicos de satisfactores residenciales  en relación a la viscosidad del tejido urbano que dan marco de referencia al  proyecto de investigación: Crecimiento y Desigualdad Urbana, periferias  internas y externas, cuyo objetivo es el desarrollo de estrategias proyectuales  para la articulación urbanística y social de las periferias -internas y  externas- de un área de la Ciudad de San Juan, Argentina, con el propósito de  modificar el actual patrón de crecimiento y desigualdad, dado por el proceso de  ocupación no sustentable de la planta urbana.La noción de tejido  refiere a la superposición de diferentes indicadores sociales, culturales,  económicos, morfológicos, ecológicos entre otros, vinculados con el concepto de variabilidad o viscosidad en  el marco de la dialéctica entre el espacio físico y espacio vivido procurando  complementar la visión objetiva con la subjetiva de la realidad. La presencia de focos de  degradación y marginalidad que conviven con urbanizaciones residenciales y  complejos comerciales de gran impacto, modifican los modos de habitar y  apropiación del espacio generando segregación y fragmentación socio-espacial en  una ciudad con predominio del mercado inmobiliario frente a políticas débiles  del estado. Los satisfactores buscan  interpretar necesidades y preferencias de los habitantes en relación al  bienestar urbano, identidad y apego al lugar; concibiendo las periferias como  interfases que abren posibilidades de ocupación sustentable, capaces de dar respuesta  a la complejidad social-espacial de la ciudad contemporánea.","palabras_clave":"Periferia|Uso Residencial|Satisfactores"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1150,"idioma_id":5,"titulo":".- Flora          Vascular de Humedales Permanentes y transitorios Bonaerenses (Buenos Aires,          Argentina).","resumen":"Vascular flora of permanent and temporary wetlands from Buenos Aires (Argentina).   This paper presents an analysis of phytocoenoses and updated lists of the vascular flora (with the exception of Poaceae) of two permanent wetlands, La Balandra and Isla Santiago, and several temporary wetlands (Arroyo Rodriguez, Arroyo El Gato, and others) in the coastal areas of the Rio de La Plata (Buenos Aires Province, Argentina). The study was based on field work and monitoring seasonal wild communities between years 2005 and  2011, with 125 vascular plant taxa identify during the period. Dicotyledons represent the dominant group in both wetlands, followed in number by Monocotyledons and a few Pteridophyta representatives. In the wetland of La Balandra 102 species of vascular plants, including 32 families of Dicotyledons (53 genera), 17 of Monocotyledons (24 genera) and 5 belonging to Pteridophyta (5 genera) were recorded, while in Isla Santiago wetland a total of 116 species were collected belonging to 34 Dicotyledon families (62 genera),17 to Monocotyledons (27 genera) and 6 to Pteridophyta (7 genus). The phytocoenoses of permanent and temporary wetlands were compared with recorded biodiversity in protected coastal areas, such as the Reserva Provincial de Punta Lara. The biodiversity of Isla Santiago is clearly larger than La Balandra, and is seriously threatened by anthropogenic activity and progress in the zone as a residential area. The temporary wetlands biodiversity is the most diminished in relation to pristine flora, and vulnerability is increasing every year.","palabras_clave":"HUMEDALES|FLORA|FITOCENOSIS|BIODIVERSIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1151,"idioma_id":4,"titulo":"Winding Distribution Effects on Induction Motor Rotor Fault Diagnosis","resumen":"The sidebands around stator currents harmonics as a potential tool for supporting the diagnosis of rotor faults in induction motors are analyzed in this paper. The presence of broken bars introduces high frequency components in the machine currents spectrum in addition to the characteristic sidebands around the fundamental component. These additional components are due to the interaction between, rotor asymmetry and either the voltage harmonics, or winding distribution, or rotor slots. In particular, the components at frequencies near to fifth and seventh harmonics, produced by the interaction between the rotor faults and the harmonics of the spatial distribution of stator windings, are analyzed in this work. A multiple coupled circuit model of the induction motor is used to evaluate the sensitivity of these components for different stator winding configurations, load level, supply voltage conditions, and different number of broken bars. Simulation results showed that a particular analyzed component near to fifth harmonic depends mainly on fifth harmonic of winding distribution, which remains almost constant for most common distributions. Therefore, it is expected that this component should be found in most motors with broken bars. Finally, experimental laboratory results and two industrial cases that validate the analysis are presented.","palabras_clave":"Broken bars|Induction motor|Winding distribution|Fault diagnosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1153,"idioma_id":4,"titulo":"Vibrational  study  of  caffeic  acid  phenethyl  ester,  a  potential anticancer agent, by infrared, Raman, and NMR spectroscopy","resumen":"The structural and vibrational properties of caffeic acid phenethyl ester (CAPE) were studied usinginfrared  and  Raman  spectroscopy  in  the  solid  phase  and  multidimensional  nuclear  magnetic  resonance(NMR)  spectroscopy  in  solution.  The  theoretical  structures  of  the compound  and  of  its  dimer  in  the  gas phase  and in  DMSO solution  by  using  density  functional  theory  (DFT)  were  studied.  The harmonic  vibrational  frequencies  for  the  optimized  geometry  of  CAPE  and  its  dimeric  species  were  calculated  at  the B3LYP  level of  theory  using  the  6?31G*  basis  set.  These  data  allow  a  complete  assignment  of  the  vibration  modes  of  the  FTIR  and  Raman  spectra  in  the  solid  state  using  the  scaled  quantum  mechanical  force &#64257;eld SQMFF)  methodology.  The  vibrational  analysis  for  the  dimer  was  performed  taking  into  account  the correlation  diagram  by  means  of  the  factor  group  analysis  in  accordance  with  the  experimental  structure determined by  X-ray  diffraction.  The  presence  of  the  dimer  of  CAPE  is  supported  by  the  IR  bands  at  1654,1635,  1563,  1533,  1300,  1107,  1050,  738  cm&#8722;1 and  the  Raman  bands  at  1684,  1681,  1634,  1112,  1050,928, 873,  850,  740,  445,  371  and  141  cm&#8722;1.  The  calculated 1H  and 13C  chemicals  shifts  are  consistent with  the  corresponding  experimental  NMR  spectra  of  the  compound  in  solution.  In  addition,  a  naturalbond orbital (NBO)  study  revealed  the  characteristics  of  the  electronic  delocalization  of  the  stable  structure,  while  the  corresponding  topological  properties  of  the  electronic  charge  density  were  analyzed  by employing  Bader?s atoms  in  the  molecules  theory  (AIM).","palabras_clave":"CAFFEIC ACID PHENETHYL ESTER|VIBRATIONAL SPECTRA|MOLECULAR STRUCTURE|FORCE FIELD|DFT CALCULATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1154,"idioma_id":4,"titulo":"Enzymatic hydrolysis of steryl glucosides, major contaminants of vegetable oil-derived biodiesel","resumen":"Biodiesels are mostly produced from lipid transesterification of vegetable oils, including those from soybean, jatropha, palm, rapeseed, sunflower, and others. Unfortunately, transesterification of oil produces various unwanted side products, including steryl glucosides (SG), which precipitate and need to be removed to avoid clogging of filters and engine failures. So far, efficient and cost-effective methods to remove SGs from biodiesel are not available. Here we describe for the first time the identification, characterization and heterologous production of an enzyme capable of hydrolyzing SGs. A synthetic codon-optimized version of the lacS gene from Sulfolobus solfataricus was efficiently expressed and purified from Escherichia coli, and used to treat soybean derived biodiesel containing 100 ppm of SGs. After optimizing different variables, we found that at pH 5.5 and 87 °C, and in the presence of 0.9 % of the emulsifier polyglycerol polyricinoleate, 81 % of the total amount of SGs present in biodiesel were hydrolyzed by the enzyme. This remarkable reduction in SGs suggests a path for the removal of these contaminants from biodiesel on industrial scale using an environmentally friendly enzymatic process.","palabras_clave":"BIODIESEL|STERYL|GLUCOSIDE|GLYCOSIDASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1155,"idioma_id":4,"titulo":"Preparation and characterization of self assembled monolayers of 2-mercaptonicotinic acid on Au(111)","resumen":"Abstract: The characterization by cyclic voltammetry, impedance spectroscopy and SERS measurements of 2-mercaptonicotinic acid (2-MNA) monolayers formed under different conditions on Au(111) surfaces, is reported.  Self-assembled 2-MNA monolayers on Au(111) in alkaline solutions desorbs reductively from the gold surface at -0.76 V (vs. ECS). From desorption experiments, the value of the surface concentration was   estimated, resulting similar to that reported for related aromatic molecules. After 30 min of dipping time 2-MNA layers are closely packed on the Au(111) surface and the structure of the SAM is stabilized.    Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed for obtaining structural information of the monolayers by analysis of the change of the kinetics of the electron transfer reaction for the Fe2+\/Fe3+ redox probe on the SAM. The results of CV and EIS show a rather imperfect blocking behaviour of the 2-MNA SAM and the Fe2+\/Fe3+ redox process behaves as a more irreversible electron transfer reaction. This behaviour corresponds to that obtained for microelectrode arrays, which is attributed to the access of ions through pinholes, defects and\/or pores present in the SAM. From EIS experiments, the estimation of coverage can be made.  In aqueous solution, 2-mercaptonicotinic acid behaves as a weak acid presenting a complex acid-base behaviour as well as equilibrium reactions between tautomeric species, including the formation of zwitterionic forms. The results can be explained taking into account that in strong alkaline aqueous solutions, 2-MNA stable species corresponds mainly to the doubly-charged negative species and a   strong thiolate-gold chemical bond as well as a moderate interaction involving the non-bonding electrons of the non-protonated pyridinic nitrogen with the gold surface are expected. Under these conditions, no interaction of the gold surface with the carboxylate group is detected by SERS, which means that the carboxylate group is exposed to the solution.    The characteristics of ionisable groups exposed to the solution in the 2-MNA monolayers prepared by dipping in alkaline solutions were obtained by impedance measurements at different pH using the [Fe(CN)6]3-\/[Fe(CN)6]4-redox probe and the value of pKa(SAM) for the carboxylate group in the   surface 2-MNA monolayer was estimated. For pH > pKa, the carboxylic acid group is deprotonated and the monolayer acquires a negative surface charge, meanwhile it remains neutral or positively charged at lower pH values due to the beginning of pyridinic nitrogen protonation, which takes place overlapped with the protonation of the carboxylate group. The protonation of the pyridinic nitrogen is accompanied by desorption of this group from the surface, allowing the formation of the zwitterionic   species for of 2-MNA by intramolecular proton transfer between the nitrogen of the ring and the carboxylic acid group. This leads to a change in the orientation of 2-MNA species allowing the interaction between the carboxylate group and the gold surface, which was further corroborated from SERS measurements.   Reductive desorption of SAMs of thiols at different pH values was also employed to evaluate properties such as the pKa values of the different functional groups in the adsorbed molecules. As pH of the electrolyte is lowered, protonation of the thiolate group and\/or any other ionisable groups in the molecule may take place after the desorption process, resulting in a pH-dependent value of peak potential for reductive desorption.  In alkaline aqueous medium, 2-MNA adsorbs through a thiolate chemical bond on the gold surface and an additional interaction of the pyridinic nitrogen stabilizes the monolayer. Thus, when reductive   desorption is made in alkaline medium, only one cathodic peak is obtained. Furthermore, the splitting in two cathodic current contributions for pH values lower than ca. 7, allows to state that two different molecular configurations for 2-MNA coexist on the gold surface in neutral or acidic media. This is assigned to the occurrence of an equilibrium between the molecular structure bonded through sulphur   and nitrogen and the surface-bonded zwitterionic species by intramolecular proton transfer from the carboxylic acid group to the ring nitrogen, leading to the protonation of the pyridinic nitrogen and   allowing the interaction of the carboxylate group with the gold surface. pKa values of the adsorbed and\/or dissolved species for 2-MNA adsorption onto Au(111) were estimated from reductive desorption experiments performed at different pH values.","palabras_clave":"MERCAPTONICOTINIC ACID|THIOL|SAM|GOLD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1156,"idioma_id":5,"titulo":"Influencia de las Condiciones de Operación sobre el Comportamiento de una Celda de Combustible PEM de H2\/O2 a Escala Prototipo","resumen":"Se presentan resultados de la evaluación del comportamiento en operación de una celda de combustible unitaria PEM de hidrógeno\/oxígeno a escala prototipo, construida con electrodos porosos de difusión de gas desarrollados en el laboratorio con incorporación de nanopartículas de Pt policristalinas soportadas sobre Vulcan XC-72. Las curvas de polarización muestran los perfiles típicos donde se distinguen las diferentes zonas de pérdidas de energía asociadas a los fenómenos limitantes. Se estudia la influencia de la  variación de la temperatura de operación de la celda de combustible y de la humidificación de los gases de alimentación sobre su desempeño y se propone un método de activación de los electrodos porosos de difusión de gas utilizados en la celda de combustible prototipo.","palabras_clave":"CELDA DE COMBUSTIBLE PEM|HIDRÓGENO|NANOPARTÍCULAS DE PT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1157,"idioma_id":4,"titulo":"Conductivity in SnO2 polycrystalline thick film gas sensors:  tunneling electron transport and oxygen diffusion","resumen":"Conduction mechanisms in polycrystalline SnO2thick sensing films were investigated by means of DCelectrical resistance during heating?cooling cycles. Samples were maintained at relatively high temper-atures in H2or O2ambient atmospheres before performing electrical measurements under vacuum orbefore performing XPS measurements in order to determine band bending. Results suggest that inter-grains present Schottky barriers that are responsible for the observed conductivities regardless of gaspre-treatment. Oxygen diffusion modulates barrier widths affecting conductivity through tunnelingtransport. The electrical response to subsequent exposure to an oxygen atmosphere is consistent withour interpretation.","palabras_clave":"TIN OXIDE|ELECTRON TUNNELING|OXYGEN DIFFUSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1159,"idioma_id":5,"titulo":"Balance energético del comportamiento de un sistema fotovoltaico instalado en una vivienda residencial conectado a la red eléctrica de distribución","resumen":"En la  Provincia de San Juan el Gobierno Provincial viene desarrollando el Proyecto  Solar San Juan que pretende fundar las bases para el crecimiento de la  tecnología fotovoltaica en todos sus tópicos, desde la producción del silicio  grado solar, la fabricación de módulos fotovoltaicos y la inserción en el  Sector Residencial, Comercial, Industrial, Bombeo de Agua y en el montaje de  Centrales de Generación de Energía Solar Fotovoltaica. En este trabajo se  presentan los avances realizados y resultados preliminares obtenidos en el  desempeño del comportamiento del sistema fotovoltaico instalado en una vivienda  del sector residencial del departamento de Caucete, Provincia de San Juan. Se  presenta inicialmente la configuración del sistema fotovoltaico; punto de  conexión, estructuras soportes, equipamiento y componentes del sistema  fotovoltaico. Por último se presentan los resultados preliminares en cuanto al  comportamiento eléctrico del sistema fotovoltaico y el balance energético del  conjunto sistema fotovoltaico, consumo de la vivienda y red eléctrica","palabras_clave":"Generación Distribuida|Generación fotovoltaica|Conexión a red|Balance energético"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1160,"idioma_id":4,"titulo":"Motorcycle Helmet Use in Mar del Plata, Argentina: Prevalence and Associated Factors.","resumen":"Injuries resulting from motorcycle crashes constitute a growing problem in Argentina and other Latin American countries. The problem is aggravated because helmet use is not widespread. This observational study analysed the prevalence of helmet use and related factors in a city in Argentina. The sample consisted of 2542 observations of motorcyclists. The results show an incidence of helmet use of 69.8% for drives and 43.4% for passengers. Helmet use was greater among women. Environmental and temporal conditions were related with the rate of helmet use. The findings indicate a considerable increase in helmet use with respect to prior years, providing evidence in favour of government policies. However, the number of motorcycles in circulation has tripled in the past five years, and therefore, the public health impact of injuries due to motorcycle crashes persists.","palabras_clave":"protective devices|helmet|motorcycles|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1162,"idioma_id":4,"titulo":"Use of hairy roots extracts for 2,4-DCP removal and toxicity evaluation by Lactuca sativa test","resumen":"Abstract 2,4-Dichlorophenol (2,4-DCP) is widely distributed  in wastewaters discharged from several industries, and it is  considered as a priority pollutant due to its high toxicity. In  this study, the use of different peroxidase extracts for 2,4-DCP  removal from aqueous solutions was investigated. Tobacco  hairy roots (HRs), wild-type (WT), and double-transgenic  (DT) for tomato basic peroxidases (TPX1 and TPX2) were  used to obtain different peroxidase extracts: total peroxidases  (TPx), soluble peroxidases (SPx), and peroxidases ionically  bound to the cell wall (IBPx). All extracts derived from DT  HRs exhibited higher peroxidase activity than those obtained  from WT HRs. TPx and IBPx DT extracts showed the highest  catalytic efficiency values. The optimal conditions for 2,4-  DCP oxidation were pH 6.5, H2O2 0.5 mM, and 200 U mL&#8722;1  of enzyme, for all extracts analyzed. Although both TPx  extracts were able to oxidize different 2,4-DCP concentrations,  the removal efficiency was higher for TPx DT. Polyethylene  glycol addition slightly improved 2,4-DCP removal  efficiency, and it showed some protective effect on TPx WT  after 2,4-DCP oxidation. In addition, using Lactuca sativa  test, a reduction of the toxicity of post removal solutions was  observed, for both TPx extracts. The results demonstrate that  TPx extracts from both tobacco HRs appear to be promising  candidate for future applications in removing 2,4-DCP from  wastewaters. This is particularly true considering that these  peroxidase sources are associated with low costs and are  readily available. However, TPx DT has increased peroxidase  activity, catalytic efficiency, and higher removal efficiency  than TPx WT, probably due to the expression of TPX1 and  TPX2 isoenzymes","palabras_clave":"REMOVAL|2,4-DCP|HAIRY ROOTS|POLYETHYLENE GLYCOL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1163,"idioma_id":5,"titulo":"GLOSARIO DE ESTRUCTURA SOCIAL","resumen":"Se trata de un Glosario de uso de términos frecuentes en Estructura Social.&nbsp;","palabras_clave":"ESTRUCTURA SOCIAL |ARGENTINA|CLASES SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1164,"idioma_id":4,"titulo":"Hepatitis B virus and hepatitis D virus in blood donors from Argentina: Circulation of HBsAg and reverse transcriptase mutants","resumen":"In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore\/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case. © 2013 Springer-Verlag Wien.","palabras_clave":"HEPATITIS B|HEPATITIS D|BLOOD DONORS|MUTANTS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1167,"idioma_id":10,"titulo":"Responsabilidad Social Universitaria en la Promoción de la Salud","resumen":"Siguiendo con el propósito de fortalecer las intervenciones de la   Universidad en la Sociedad, desde una perspectiva que permite interpelar los   modelos de formación profesional y la vinculación de la institución superior con la   sociedad, en el encuentro de diferentes saberes que contribuyan a garantizar la   Responsabilidad Social Universitaria (SCOTTO, 2012), el Equipo Interdisciplinario   de Investigación PROCON3 de la Facultad de Odontología de la Universidad   Nacional de Córdoba junto con la comunidad de la Escuela Profesor Miguel   Luciani de la Provincia de Córdoba compartieron el diseño e implementación de   diversos proyectos de investigación, extensión y Voluntariado Universitario, que   dieron lugar a la experiencia que presentamos.  La experiencia se concreta en los año 2010-2012 en la escuela Profesor   Miguel Luciani, perteneciente a una comunidad urbana vulnerable de la ciudad de   Córdoba, a partir de los lineamientos de la Investigación con Intervención y el   Aprendizaje en Servicio. La misma tuvo como marco el Proyecto de Voluntariado   Universitario  : Promoción de salud bucal. Cuido mis dientes comiendo rico, sano   y barato?, subsidiado por la Secretaria de Políticas Universitarias de la Presidencia   de la Nación.","palabras_clave":"RESPONSABILIDAD SOCIAL UNIVERSITARIA  |PROMOCION DE SALUD   |APRENDIZAJE EN SERVICIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1168,"idioma_id":4,"titulo":"In silico identification of the active conformation of open-chain Enaminones with anticonvulsant activity","resumen":"A study about the relationship between molecular properties of open-chain enaminones and their anticonvulsant activity is presented in this paper. Geometry optimizations of the enaminones were performed at HF and DFT\/B3LYP levels of theory using 6-31 ? G(d) basis set. The HOMO and LUMO energies were obtained at thesame level of theory. The solvent effect was studied through IPCM. A natural bond orbital (NBO) analysis was performed to analyze the possible association between the stability and the intramolecular hydrogen bond interaction energies. The stability order of the isomers in gas phase was the following: cis-1[trans-4[cis-2[trans-3. The IPCM method showed that the trans-3 isomers are more stable that the cis-2 when the solvent effect was taken into account. Two important intramolecular hydrogen bonds were found by NBO analysis. According to our findings, these interactions could affect the activity of the two most stable isomers (cis-1 and trans-4). By contrast, trans-3 isomers did not present this type of interaction. Therefore, the latter isomers have a large flexibility and can adopt a conformation similar to the conformation of active ringed enaminones. In addition, HOMO and LUMO energies suggested that the trans-3 isomers could be the most reactive species.","palabras_clave":"OPEN-CHAIN ENAMINONES|ANTICONVULSANT ACTIVITY|ACTIVE CONFORMATION|AB INITIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1170,"idioma_id":4,"titulo":"Methodology aimed at evaluating urban life quality levels","resumen":"The paper explores the theoretical and conceptual aspects of urban life quality (ULQ) synthesized in a model that includes the physical support of the cities, in our case of intermediate scales. This model considers the interactions between basic services, infrastructure and environmental aspects. Maps are shown which localize and define the state of basic needs in infrastructure, services and environment quality. The most vulnerable areas and trends in life quality are identified from the integration of different urban services and environmental aspects according to their consolidation. This allowed us to define homogeneous areas with significant differences in quality and singularities among urban services and environmental aspects. Finally, we make some methodological and operational considerations regarding advantages and limitations experienced in implementing the model.","palabras_clave":"calidad de vida urbana|modelización espacial"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1171,"idioma_id":5,"titulo":"Bienestar social y aculturación psicológica en estudiantes universitarios migrantes.","resumen":"El objetivo del trabajo es el  de analizar las estrategias de aculturación psicológica y la percepción de  bienestar social en estudiantes universitarios migrantes. El estudio es de tipo  descriptivo de diferencias de grupos, de diseño transversal. La muestra es no  probabilística intencional compuesta por 214 estudiantes universitarios migrantes  externos e internos de ambos sexos con edades entre 18 y 35 años residentes en  la Ciudad de Buenos Aires. Los resultados muestran el predominio de estrategias  de aculturación de integración y separación, y niveles medios de bienestar  social. Asimismo, los estudiantes migrantes externos muestran mayor predominio  de estrategias de asimilación e integración y los internos estrategias de  separación tanto en el ámbito público como privado.","palabras_clave":"ACULTURACIÓN|BIENESTAR SOCIAL|ESTUDIANTES UNIVERSITARIOS|MIGRANTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1172,"idioma_id":4,"titulo":"Ignition probability of fine dead surface fuels in native Patagonian forests of Argentina.","resumen":"&nbsp;Aim of study: The Canadian Forest Fire Weather Index (FWI) is being implemented all over the world. This index is being adapted to the Argentinean ecosystems since the year 2000. With the objective of calibrating the Fine Fuel Moisture Code (FFMC) of the FWI system to Patagonian forests, we studied the relationship between ignition probability and fine dead surface fuel moisture content (MC) as an indicator of potential fire ignition.Area of study: The study area is located in northwestern Patagonia, Argentina, and comprised two main forest types (cypress and ñire) grown under a Mediterranean climate, with a dry summer and precipitations during winter and autumn (~500-800 mm per year).Material and Methods: We conducted lab ignition tests fires to determine the threshold of fine dead fuel ignition at different MC levels. Moisture content of dead fine surface fuels in the field was measured every 10-15 days from November to March for three seasons. We calculated the FFMC during these seasons and correlated it with the measured MC by applying a logistic regression model. We combined the results of the ignition tests and of the regressions to suggest FFMC categories for estimating fire danger in Patagonian forests.Main results: The ignition threshold occurred at MC values of 21.5 and 25.0% for cypress and ñire sites, respectively. The MC measured varied from 7.3 to 129.6%, and the calculated FFMC varied between 13.4 and 92.6. Highly significant regressions resulted when FFMC was related to MC. The ignition threshold corresponded to a FFMC=85. We proposed to divide the FFMC scale in three fire danger categories: Low (FFMC&lt;=85), High (85&lt;FFMC&lt;=89) and Extreme (FFMC&gt;89).Research highlights: Our results provide a useful tool for predicting fire danger in these ecosystems, and are a contribution to the development of the Argentinean Fire Danger Rating and a reference for similar studies in other countries where the FWI is being implemented.","palabras_clave":"WILDFIRE|FIRE BEHAVIOR|FUEL MOISTURE|FIRE WEATHER INDEX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1176,"idioma_id":4,"titulo":"Kinetics of bioaccumulation of heavy metals in Odontesthes bonariensis is explained by a single and common mechanism","resumen":"Fish are often considered a very sensitive indicator of heavy metal contamination in aquatic ecosystems;however, the detailed mechanisms of bioaccumulation remain unclear. Here, we study the bioaccu-mulation processes of three relevant heavy metals (Cd2+, Cu2+and Cr6+) in Odontesthes bonariensis usingmathematical modelling. We developed a general compartmental kinetic model that describes the trans-port of heavy metals between the surrounding water and the gills and liver of fish. The general modelwas reduced to a simple one still capable of reproducing previous experimental data and suggesting acommon mechanism for the three metals. The modelling results indicate that bioaccumulation of Cd2+,Cu2+, and Cr6+is described by a combination of a concentration-independent and saturable uptake kinet-ics in both organs with a unidirectional path of elimination from gills to liver to waterborne. Finally, thegood agreement between the parameter values predicted by the model and previously published datasuggests that our modelling approach may shed light on the mechanisms of heavy metal bioaccumulationin other species.","palabras_clave":"Mathematical modelling|modellings|Odontesthes bonariensi|Bioaccumulation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1177,"idioma_id":5,"titulo":"Origen floral, contenido mineral y color de mieles de distintas localidades de Catamarca.","resumen":"2XX","palabras_clave":"x"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1178,"idioma_id":4,"titulo":"implementation of several mathematical algorithms to breast tissue density classification","resumen":"  \t\t\t    \t\t\t\t    \t\t\t\t\t  The accuracy of mammographic abnormality detection methods is strongly dependent on breast tissue  characteristics, where a dense breast tissue can hide lesions causing cancer to be detected at later stages.  In addition, breast tissue density is widely accepted to be an important risk indicator for the  development of breast cancer. This paper presents the implementation and the performance of different  mathematical algorithms designed to standardize the categorization of mammographic images, accord-  ing to the American College of Radiology classifications. These mathematical techniques are based on  intrinsic properties calculations and on comparison with an ideal homogeneous image (joint entropy,  mutual information, normalized cross correlation and index Q) as categorization parameters. The  algorithms evaluation was performed on 100 cases of the mammographic data sets provided by the  Ministerio de Salud de la Provincia de Co&#769;rdoba, Argentina?Programa de Prevencio&#769;n del Ca&#769;ncer de Mama  (Department of Public Health, Co&#769;rdoba, Argentina, Breast Cancer Prevention Program). The obtained  breast classifications were compared with the expert medical diagnostics, showing a good performance.  The implemented algorithms revealed a high potentiality to classify breasts into tissue density categories.    \t\t\t\t  \t\t\t  \t\t","palabras_clave":"Breast density classification|Mathematical processing|Computer-aided diagnostic systems|Mammography"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1180,"idioma_id":4,"titulo":". Laurel leaf extracts for honeybee pest and disease management: antimicrobial, microsporicidal, and acaricidal activity","resumen":"Abstract A diverse set of parasites and pathogens affects  productivity and survival of Apis mellifera honeybees. In  beekeeping, traditional control by antibiotics and molecules  of synthesis has caused problems with contamination and  resistant pathogens. In this research, different Laurus nobilis  extracts are tested against the main honeybee pests through an  integrated point of view. In vivo effects on bee survival are  also evaluated. The ethanol extract showedminimal inhibitory  concentration (MIC) values of 208 to 416 &#956;g\/mL, having the  best antimicrobial effect on Paenibacillus larvae among all  substances tested. Similarly, this leaf extract showed a significant  antiparasitic activity on Varroa destructor, killing 50 %  of mites 24 h after a 30-s exposure, and on Nosema ceranae,  inhibiting the spore development in the midgut of adult bees  ingesting 1×104 &#956;g\/mL of extract solution. Both ethanol  extract and volatile extracts (essential oil, hydrolate, and its  main component) did not cause lethal effects on adult honeybees.  Thus, the absence of topical and oral toxicity of the  ethanol extract on bees and the strong antimicrobial,  microsporicidal, and miticidal effects registered in this study  place this laurel extract as a promising integrated treatment of  bee diseases and stimulates the search for other bioactive  phytochemicals from plants.","palabras_clave":"Apis mellifera|Laurus nobilis|Varroa destructor|Paenibacillus larvae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1181,"idioma_id":4,"titulo":"Using Morphometry and Molecular Markers for Sexing South  American, Cayenne and Royal Terns Breeding in Patagonia, Argentina","resumen":"The first information on the morphometry and sexual size dimorphism of the sympatric South  American (Sterna hirundinacea), Cayenne (Thalasseus sandvicensis eurygnathus) and Royal (T. maximus maximus)  terns from the Patagonia region in Argentina is provided, and a discriminant analysis to sex the three species is  used. Morphological characters were obtained from South American Terns (n = 83), Cayenne Terns (n = 63) and  Royal Terns (n = 20). All species were sexed using polymerase chain reaction-based molecular techniques. Sexes in  the three tern species were only slightly dimorphic in size. Male Cayenne and South American terns were significantly  larger than females in bill length, bill depth and head length. Royal Terns, in contrast, showed a high overlap  in most morphological measurements, with head length being the only measurement that differed significantly  between sexes. Head length correctly sexed 89% of South American Terns and 75% of Royal Terns, while a function  including bill depth and head length correctly sexed 78% of Cayenne Terns. Our results provide a valuable tool  for rapid sexing in the field of these three Patagonian terns, although reliable sexing in Cayenne and Royal terns  should be preferentially achieved using a combination of morphometric and molecular sexing","palabras_clave":"DISCRIMINANT ANALYSIS|MORPHOMETRIC TRAITS|SEX DETERMINATION|PATAGONIAN TERNS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1184,"idioma_id":4,"titulo":"Characterization of the Bahia Blanca estuary by data analysis and numerical modelling.","resumen":"The Bahía Blanca Estuary is a complex system of channels and tidal flats  where the most important deep water harbour system of Argentina is located.  The main goal of the present work was to obtain a hydrodynamic conceptual  model for the Bahía Blanca coastal area. For this reason, a combined analysis  of observed data and numerical modelling has been performed for the whole  area. The gained knowledge on the system hydrodynamics could aid in the  decision support for navigation security, waste water discharges management,  sediment dredging and rejection operations among other applications.  Due to the Bahia Blanca coastal vast area, hydrodynamic observations  are scarce and located near the populated areas. In order to describe the  hydrodynamics of such a complex and large system, the analysed tidal and  current data from different periods have been completed through numerical  modelling.  Data analysis served to determine the main processes governing the Bahia  Blanca hydrodynamics, to characterise the area using general descriptors, to  provide inputs for the numerical model and aid to evaluate its performance.  In addition, a 2-Dimensional application was set up using the MOHID water  modelling system for the Bahía Blanca estuary. This application aimed to gain a better understanding of the system dynamics, to explain and test the  consistency of the observed data and to reproduce the processes taking place.  Model results were in good agreement with the analysed data and served  to confirm an inconsistency found on the sea level observations. The combination  of both methodologies served to further describe the hydrodynamic  processes governing this coastal area and also to obtain a conceptual model  for the water and properties circulation in the Bahía Blanca estuary.","palabras_clave":"Bahía Blanca estuary,|hydrodynamic model|Mohid numerical model"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1190,"idioma_id":4,"titulo":"Identification of proteins involved in Hg-Se antagonism in water hyacinth (Eichhornia crassipes)","resumen":"x","palabras_clave":"proteins |Hg-Se antagonism|Eichhornia crassipes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1191,"idioma_id":5,"titulo":"Situación sanitaria del cultivo de garbanzo en la provincia de Tucumán y detección de rabia (Ascochyta rabiei) en la provincia de Catamarca.","resumen":"El garbanzo (Cicer arietinum L.) es una leguminosa invernal cuyo cultivo comenzó a extenderse en la provincia de Tucumán en los últimos siete años. La superficie sembrada con garbanzo tuvo un crecimiento abrupto, especialmente en las últimas dos campañas, alcanzando 25.960 ha en 2011 y 28.080 ha en 2012. El rendimiento promedio alcanzado fue del orden de 1,8 t\/ha en la campaña 2011, en la cual las condiciones ambientales fueron óptimas para el desarrollo del cultivo a lo largo de todo su ciclo. Al ser un cultivo que se está expandiendo por nuevas zonas, existen aspectos relativos a su producción que todavía requieren mayor conocimiento y desarrollo tecnológico local. Los aspectos sanitarios del cultivo no escapan a estas consideraciones. Las enfermedades del garbanzo, debido a las pérdidas que pueden llegar a ocasionar en los cultivos, deben ser tenidas en cuenta en los esquemas de manejo de esta leguminosa. Es importante siempre tener presente la fuerte influencia que tienen las condiciones ambientales en la ocurrencia y desarrollo de las patologías vegetales y el hecho de que estas condiciones pueden hacer variar los niveles de incidencia de las enfermedades año a año. En el presente trabajo, se resumen las principales características de las enfermedades de garbanzo y se presentan los resultados de las prospecciones del estado sanitario del cultivo durante los años 2010, 2011 y 2012, en la provincia de Tucumán y zonas agrícolas de influencia.","palabras_clave":"CICER ARIETINUM|ENFERMEDADES DEL GARBANZO|FUSARIOSIS|SECA DEL GARBANZO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1193,"idioma_id":4,"titulo":"Palynological analysis of camelid coprolites: seasonality in the use of the site Cerro Casa de Piedra 7 (Santa Cruz, Argentina)","resumen":"Palynological, palaeoparasitological and paleobotanical studies of coprolites found in archaeological sites from Perito Moreno National Park (47°57&#8242;S72°05&#8242;W) yielded information on diet, palaeoenvironment and health. These studies allowed adding evidence to the reconstruction of life history of the hunter?gatherers that inhabited Patagonia during the Holocene. We examined the season of the year when camelid Lama guanicoe coprolites (5400 ± 64 yr 14C BP to 9640 ± 190 yr 14C BP) were deposited at Cerro Casa de Piedra 7 (site CCP7). The study used palynological evidence and comparison with pollen spectra of modern feces collected during summer, fall, winter and spring of 2010. The dominant types were: pollen of Nothofagus, Empetrum rubrum, Asteraceae subfam. Asteroideae, Nassauvia, Caryophyllaceae and Poaceae; fern spores; remains of Eimeria macusaniensis; and plant remains of Poaceae, Festuca pallescens, Stipa speciosa, Armeria maritima, Gaultheria mucronata and E. rubrum. Pollen spectra of modern and fossil feces were used for multivariate analysis. Coprolites associated to fall and winter modern feces. These results and those obtained from pollen concentration values and the presence of pollen types indicators of seasonality, allowed the determination of summer, fall and winter coprolites. However, caution must be taken with the seasonality results of coprolites dated earlier than 9000 years BP since the environmental conditions differed from now. The site was probably a camelid shelter during the unfavorable seasons.","palabras_clave":"POLLEN|COPROLITES|SEASONALITY|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1194,"idioma_id":4,"titulo":"Aboveground biomass patterns of the dominant Spartina species and their relationship with abiotic variables in the SW Atlantic marshes","resumen":"Salt  marsh zonation patterns generate different abiotic and biotic conditions that  can accentuate species inherent differences in primary production and biomass.  In SouthWest Atlantic marshes, there are two Spartina species: Spartina  alterniflora in the low intertidal and Spartina  densiflora in the high intertidal. These two species are generally found in  all marshes but with different dominance: In some marshes, the S. densiflora  zone occupies higher extents, and in others, the S. alterniflora zone is the one that prevails. We found through  field sampling that, in six studied marshes, there is greater S. densiflora  live and total (i.e. dead+live) aboveground biomass (g m-2) in the  marshes dominated by S. densiflora than in the ones dominated by S. alterniflora. Spartina alterniflora had similar aboveground biomass in the six  marshes, regardless of the dominance of each species. When comparing the two Spartina species within each marsh, S.  densiflora had greater live and total biomass in the marshes it dominates. In  the marshes dominated by S. alterniflora,  both species had similar live and total biomass. In all marshes, there was  greater dead S. densiflora biomass. A multivariate analysis using selected  abiotic factors (i.e., salinity, latitude, and tidal amplitude) showed that S. alterniflora aboveground biomass  patterns are mainly correlated with salinity, while S. densiflora live biomass is mainly correlated with salinity and  latitude, dead biomass with salinity and tidal amplitude, and total biomass  with salinity alone. We conclude that in S. densiflora dominated marshes, the  main processes of that species zone (i.e., nutrient accumulation) will be  accentuated because of its higher biomass. We also conclude that climatic  conditions, in combination with specific Spartina  biotic and ambient abiotic parameters, can affect marsh ecological functions.","palabras_clave":"ABIOTIC VARIABLES|ABOVEGROUND BIOMASS|REGIONAL PATTERNS|SPARTINA ALTERNIFLORA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1196,"idioma_id":4,"titulo":"Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice.","resumen":"Neonatal androgenization masculinizes the GH axis and thus may impact on liver gene regulation. Neonatal testosterone administration to female mice decreased (defeminized) female predominant GH-dependent liver gene expression (Hnf6Adh1PrlrCyp3a41) and did not modify male predominant genes(Cyp7b1Cyp4a12Slp). Female predominance of Cis mRNA, an inhibitor of episodic GH signaling pathway,was unaltered. At birth,Cyp7b1promoter exhibited a higher methylation status in female livers, while theHnf6 promoter was equally methylated in both sexes; no differences in gene expression were detected atthis age. In adulthood, consistent with sex specific predominance, lower methylation status was deter-        mined for theCyp7b1promoter in males, and for theHnf6promoter in females, and this last differencewas prevented by neonatal androgenization. Therefore, early steroid treatment or eventually endocrinedisruptor exposure may alter methylation status and sexual dimorphic expression of liver genes, and consequently modify liver physiology in females.","palabras_clave":"GH|EPIGENETICS|LIVER|PROMOTERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1197,"idioma_id":4,"titulo":"Gas phase dehydration of glycerol over Lanthanum-modified Beta-zeolite.","resumen":"Glycerol dehydration to acrolein was studied using beta zeolites. Three catalysts were used, the parent  catalyst in the protonic form (H-B), and this catalyst after two ion exchanges, both with lanthanum  nitrate (LCL-B) and one with lanthanum nitrate and the second with ammonium nitrate (LCH-B). The  catalysts were characterized by XRD, XPS, FTIR with pyridine adsorption, pyridine TPD, BET, SEM, ICP,  and pulses of 1-butene. Coke was characterized by TPO. Activity tests were carried out in gas phase. It was  found that LCH-B and H-B catalysts had similar density of acid sites, but the strength distribution was  different, having the former a higher proportion of medium-strength acid sites. Because of this, a better  performance in glycerol dehydration was displayed by this catalyst. The changes in acidity were due to  the generation of new acid sites that occurred during the preparation, forming acid silanols attached  to silica-reach and alumina-reach species. Coke was rapidly formed on the three catalysts, completely  blocking the pores. Therefore, after a short time on oil, only the external surface is available for the  reaction. Nevertheless, reasonable conversion and stability was obtained with LCH-B catalyst","palabras_clave":"acrolein|glicerol|dehydration|zeolites"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1198,"idioma_id":4,"titulo":"Memory and long-range correlations in chess games","resumen":"In this paper we report the existence of long-range memory in the opening moves of a chronologically ordered set of chess games using an extensive chess database. We used two mapping rules to build discrete time series and analyzed them using two methods for detecting long-range correlations; rescaled range analysis and  detrended fluctuation analysis. We found that long-range memory is related to the level of the players. When the database is filtered according to player levels we found differences in the persistence of  the different subsets. For high level players, correlations are stronger at long time scales; whereas in intermediate and low level players they reach the maximum value at shorter time scales. This can be interpreted as a signature of the different strategies used by players  with different levels of expertise. These results are robust against the assignation rules and the method employed in the analysis of the time series.","palabras_clave":"LONG-RANGE|CORRELATIONS|ZIP'S LAW|INTERDISCIPLINARY PHYSICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1199,"idioma_id":4,"titulo":"Key role of TLR2 in the inflammatory response and MHC-II down-regulation in Brucella-infected alveolar macrophages","resumen":"Alveolar macrophages (AM) seem to constitute the main cellular target of inhaled brucellae. Here we show that Brucella abortus invades and replicates in murine AM without inducing cytotoxicity. B. abortus infection induced a statistically significant increase of TNF-&#945;, KC, IL-1&#946;, IL-6 and IL-12 in AM from C57BL\/6 mice and Balb\/c mice, but these responses were generally weaker and\/or delayed as compared to those elicited in peritoneal macrophages. Studies using knockout mice for TLR2, TLR4 and TLR9 revealed that TNF-&#945; and KC responses were mediated by TLR2 recognition. Brucella infection reduced in a MOI-dependent manner the expression of MHC-II molecules induced by gamma-interferon (IFN-&#947;) in AM. The same phenomenon was induced by incubation with heat-killed B. abortus (HKBA) or the lipidated form of the 19 kDa outer membrane protein of Brucella (L-Omp19), and was shown to be mediated by TLR2 recognition. In contrast, no significant down-regulation of MHC-II was induced by either unlipidated Omp19 or Brucella LPS. In a functional assay, treatment of AM with either L-Omp19 or HKBA reduced the MHC-II-restricted presentation of OVA peptides to specific T cells. One week after intra-tracheal infection viable B. abortus was detected in AM from both wild type and TLR2 KO mice, but CFU counts were higher in the latter. These results suggest that B. abortus may survive in AM after inhalatory infection in spite of a certain degree of immune control exerted by the TLR2-mediated inflammatory response. Both the modest nature of the latter and the modulation of MHC-II expression by the bacterium may contribute to such survival.","palabras_clave":"BRUCELLA|ALVEOLAR MACROPHAGES|TLR2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1200,"idioma_id":4,"titulo":"Characterization of the liquid products in the pyrolysis of residual chañar and palm fruit biomasses","resumen":"The endocarp in the chañar (Geoffroea decorticans ) fruit, the pericarp in the white palm (Copernicia alba Morong) fruit and the exhausted white palm seeds from the extraction of oil are all residual lignocellu-  losic biomasses from the food processing of these fruits. The pyrolysis was performed at 550 °C in a &#64257;xed bed reactor during 30 min under N2  &#64258;ow with a 15 °C\/min heating ramp from room temperature. In all  the cases a liquid product (bio-oil and tar) with yields from 35 to 50 wt.%, a gas product and a solid product (char), were produced. The tar represented from 5 to 20 wt.% of the liquid product. The highest bio-oil  yield (47 wt.%) was obtained with the chañar fruit endocarp and the highest tar yield (8.8 wt.%) was obtained with the white palm seeds. The bio-oils contained mainly acids (from 6 to 18 wt.%), aldehydes and ketones (from 2 to 10 wt.%) and phenols (from 4 to 13 wt.%), with a high water content (from 44 to 81 wt.%). Their pH was acidic and the densities ranged from 0.97 to 1.24 kg\/dm3. The tars had mainly phenols and oxygenated compounds of high molecular weight. After these results, these residual biomasses can be considered as a potential source for energy or fuels and valuable chemical products.","palabras_clave":"Geoffroea decorticans|Copernicia alba Morong|Pyrolysis|Bio-oil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1202,"idioma_id":5,"titulo":"Multitudes católicas, sociedad de masas y política en la Argentina. Reflexiones a partir del Congreso Eucarístico Nacional de 1940","resumen":"El artículo estudia la conformación de multitudes católicas durante el Congreso  Eucarístico Nacional de 1940, celebrado en la ciudad de Santa Fe (Argentina),  desde una perspectiva sociocultural que destaca la multidimensionalidad del  fenómeno y los vínculos entre el catolicismo y la sociedad de masas. Con dicho  fin, el Congreso es abordado a través de diferentes claves: la cultura de  masas, la industria del turismo, el discurso publicitario, el consumo, la  lógica del espectáculo. Finalmente, a la luz de los resultados obtenidos, se  ensaya un análisis de la dimensión política, con el propósito de comprender el  modo en que dichos procesos alimentaron la retórica del 'mito de la nación  católica' y contribuyeron a fortalecer las posiciones de la Iglesia Católica en la política argentina.","palabras_clave":"MOVILIZACIÓN CATÓLICA|CATOLICISMO|IGLESIA|SECULARIZACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1204,"idioma_id":4,"titulo":"Presence of GH1 and absence of GHRHR gene mutations in a large cohort of Argentinian patients with severe short stature and isolated GH deficiency","resumen":"The prevalence of GH1 gene alterations is around 20% in patientswith severe isolated growth hormone deficiency (IGHD). Mutationsin the GHRHR gene are recognized as a significant cause offamilial IGHD, mainly in areas of the Indian subcontinent.1This study was designed to identify, in the Argentinian population,known and new molecular alterations in the GH1 gene ina cohort of 46 patients (41 nonconsanguineous pedigrees, 22familial cases) with IGHD, and molecular alterations in theGHRHR gene in 12 familial index cases devoid of molecularabnormalities in the GH1 gene.The study was approved by the Ethics Committee of theGarrahan Pediatric Hospital.Genomic DNA was extracted from blood according tostandard procedures. The GH1 gene was specifically PCR-amplifiedusing sense and antisense primers corresponding to nucleotides5101?5136 and the complement of nucleotides 7255?7226(NG_011676.1). The coding sequence and flanking intronicregions of the GHRHR gene were PCR-amplified using specificprimers.2 The whole GH1 and each GHRHR purifiedfragment (Qia Quick PCR purification kit, Qiagen, Buenos Aires,Argentina) were directly sequenced using BigDye Terminator version3.1 cycle sequencing kit (Applied Biosystems, Buenos Aires,Argentina) and 3130 Genetic Analyzer capillary DNA sequencer(Applied Biosystems). The nucleotide sequences obtained werecompared with those from Genebank accession numbersNG_011676.1 and NG_021416.1 for GH1 and GHRHR genes,respectively. Gene deletions of GH1 were detected by RFLP withthe restriction enzymes Sma I, Bgl I or Hae II for the detection of67, 70 and 76 deletions, as previously described.3 Simulationtests were applied to identify the potential functional impact ofnewly found variants, using online tools such as sequence homology-based tool, SIFT (Sorting Intolerant from Tolerant; http:\/\/sift.jcvi.org\/) version 2.0.6, the structure-based tool, PolyPhen2(Polymorphism Phenotyping; http:\/\/genetics.bwh.harvard.edu\/pph2) and mutation taster (http:\/\/www.mutationtaster.org\/).We identified formerly reported GH1 gene mutations, in 9\/41(22%) nonconsanguineous pedigrees (13 patients), with a higherprevalence among familial cases (364%). Clinical information,laboratory findings and molecular studies in patients and progenitorswith mutations in the GH1 gene are shown in Table 1.Analysis of GH1 and GHRHR genes revealed (i) GH1 genedeletions in three patients from two nonconsanguineous pedigrees(Table 1). In the two unrelated families, we identified a67-kb deletion in the GH1 gene. Parents were heterozygous forthe GH1 gene deletion. (ii) GH1 gene mutations in 10 patientsfrom seven nonconsanguineous pedigrees and always in oneprogenitor of the six pedigrees studied (Table 1). We identifiedtwo previously described, heterozygous, missense mutations:p.Arg183His4 in six pedigrees and p.Val110Ile in one family,described as a functional polymorphism with significantlyreduced GH1 secretion.5 (iii) GH1 gene variants: two novelmissense variations in the GH1 gene in two unrelated patients(p1, p2, Table 1). The nucleotide sequences of genomic DNA inp1 revealed a heterozygous novel variation in exon 2, substitutingC for T at cDNA nucleotide position 55, resulting in asubstitution of proline to serine at codon -7 (p.Pro-7Ser) of theGH1 protein. The nucleotide sequences of genomic DNA in p2revealed a heterozygous novel variation in exon 4, substituting Tfor C at cDNA nucleotide position 305, resulting in the variationof leucine for proline at codon 102 (p.Leu102Pro) of the matureprotein. His father had severe short stature (height SDS:-512)and was also found to be heterozygous for the same GH1 genevariation. No allele carrying this variation was found in 60control subjects (120 alleles) studied. As p.Leu102Pro affectsa highly conserved amino acid of the GH1 protein and Poly-Phen2 tool predicted it to be probably damaging, this novel variantmight be deleterious for protein activity. On the contrary,p.Pro-7Ser does not affect a conserved residue and PolyPhen2tool predicted that this variation was not deleterious for theGH1 protein, strongly suggesting a common polymorphism.Similar prediction results for both GH1 novel gene variantsusing mutation taster and SIFT tools were obtained. (iv) TheGHRHR gene, sequenced in 12 IGHD index cases with familialIGHD devoid of molecular abnormalities in the GH1 gene,showed no molecular alteration.The Garrahan Pediatric Hospital is a referral centre for thewhole country, and therefore, patients attending the hospital area representative sample of the Argentinian population. To date,at least 58 mutations have been reported in the GH1 geneassociated with IGHD, including splice site and missense mutationsand large deletions. In our cohort, type II IGHD was thecommonest genetic form. In contrast to other populations,missense mutations instead of GH1 splice site mutations werepredominant. We have identified three mutations previouslydescribed in the GH1 gene (9\/41 pedigrees, 22%) with a predominanceof p.Arg183His. In agreement with previous reportsin other populations, this percentage increased to 364%, consideringfamilial cases only (8\/22 pedigrees). In addition, in twounrelated patients, two novel heterozygous nonsynonymousexonic variants causing a change in the encoded amino acid(p.Pro-7Ser, p.Leu102Pro) were identified in the GH1 gene.Functional studies could not been carried out. However, thefunctional impact was estimated according to (i) the presence ofthe GH1 gene variant in our general population, (ii) analysis ofthe evolutionary conservation of the amino acids and (iii) applicationof simulation tests. Using this approach, it could be suggested that only the novel GH1 gene variant p.Leu102Promight be deleterious for protein activity. Interestingly, the fatherwho carried this novel GH1 gene variant has extremely shortstature (Height SDS:-512), suggesting that the p.Leu102ProGH1 gene variant might encode an inactive protein causing asevere growth disorder in the father as well. Similarly to otherreports in Caucasian populations, no mutations in the GHRHRgene were found1.In conclusion, we present the results of screening for mutationsin GH1 and GHRHR genes in a large cohort of Argentinianpatients with IGHD. These suggest that the p.Arg183Hismutation associated with the type II dominant form of IGHDmight be relatively common. We also report a novel variation(p.Leu102Pro) with strong clinical and indirect data that thisrepresents a loss-of-function mutation in the GH1 gene.","palabras_clave":"GH1|GHRHR|isolated GH deficiency"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1205,"idioma_id":4,"titulo":"Modeling of second-order spectrophotometric data generated by a pH-gradient flow injection technique for the determination of doxorubicin in human plasma","resumen":"detection combined with extendedmultivariate curve resolution?alternating least-squares (MCR?ALS) to quantitate  doxorubicin in the presence of rifampicin in plasma samples is presented.  Quantitation of doxorubicin was based on acid?base and spectral differences between its species and those for  the interference. Overlapped profiles among the analyte and the interference were mathematically solved by  data modeling with the well-known extended MCR?ALS algorithm.  The method, which involves a simple sample pretreatment consisting in the addition of trichloroacetic acid  followed by centrifugation, was applied to resolve validation mixtures composed of human plasma spiked  with doxorubicin. Acceptable analytical figures of merit were obtained, i.e. recoveries from 96 to 107%, coefficients  of variation between 0.2% and 7.8%, and limits of detection and quantification of 0.77 and 2.32 &#956;g L&#8722;1,  respectively","palabras_clave":"Multivariate curve resolution |Doxorubicin  |Human plasma|Second-order calibration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1206,"idioma_id":4,"titulo":"GTP-bound Rab3A exhibits consecutive positive and negative roles during human sperm dense core granule exocytosis","resumen":"Exocytosis of mammalian sperm´s dense-core secretory granule relies on the same fusion molecules as all other secretory cells; one such molecule is the small GTPase Rab3A. Here, we report a deep biochemical characterization of Rab3A?s role in secretion by scrutinizing the exocytotic response of streptolysin O-permeabilized human sperm to the acute application of a number of Rab3A-containing constructs and correlating the findings with those gathered with the endogenous protein. Full length, geranylgeranylated and active Rab3A elicits human sperm exocytosis per se. With Rab3A\/Rab22A chimeric proteins we demonstrate that the carboxy-terminus domain of the Rab3A molecule is necessary and sufficient to promote exocytosis whereas its amino-terminus prevents calcium-triggered secretion. Interestingly, full length Rab3A halted secretion when added after the docking of the acrosome to the plasma membrane. This effect depended on Rab3A?s inability to hydrolyze GTP. We combined modified immunofluorescence and acrosomal staining protocols to detect membrane fusion and the activation status of endogenous Rab3 simultaneously in individual cells and found that GTP hydrolysis on endogenous Rab3 was mandatory for fusion pores to open. Our findings contribute to establish that Rab3 modulates regulated exocytosis differently depending on the nucleotide bound and the exocytosis stage under study.","palabras_clave":"RAB3|EXOCYTOSIS|SPERM|CHIMAERA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1208,"idioma_id":4,"titulo":"Contribution of fishery discards to the diet of the Black-browed  albatross (Thalassarche melanophris) during the non-breeding  season: an assessment through stable isotope analysis","resumen":"Black-browed albatrosses (Thalassarche melanophris)disperse over the Argentinean Continental Shelfand neighboring waters during their non-breeding season.It is one of the most frequent seabirds attending fishingvessels and also the most common Procellariform in thebycatch of longliners and trawlers in the area. Understandingthe use of fishery discards by this species is an importantissue when assessing the potential effect of strategicdiscard management in decreasing the abundance, interactions,and mitigating mortality. In the present study, weanalyzed carbon and nitrogen stable isotope compositionsin the blood of Black-browed albatrosses to assess therelative contribution of discards from different fisheries tothe diet of this species in winter. Samples were obtainedin winter 2011 from fishing vessels operating between41?43°S and 57?59°W. No sex differences in &#948;13C and&#948;15N were observed. Results indicate that during their nonbreedingseason, isotopic signatures of Black-browed albatrossesare closer to discards and offal generated by fisheriesand in particular by trawlers. The large fishing effortof trawl fisheries in Argentina highlights the urgency of anexhaustive analysis to find practical and effective ways toreduce the number of seabirds attending trawlers.","palabras_clave":"BLACK-BROWED ALBATROSS|FISHERY DISCARDS|STABLE ISOTOPES|WINTERING|ARGENTINEAN CONTINENTAL SHELF"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1209,"idioma_id":5,"titulo":"Animales que miran y pasan (La autobiografía como zoo-grafía)","resumen":"Este trabajo se pregunta por qué el film dirigido por Safaa Fathy, D´ailleurs Derrida, puede ser pensado como un film sobre los animales. Los animales, en este film, miran y pasan. Se analizan los modos del pasaje animal desde el 'corte de lo vivo', la irrupción y los 'envois' (fragmentos de texto escritos al modo de cartas o tarjetas postales dirigidos a un destinatario indefinido), para pensar un modo de hospitalidad con el animal (modo que lo 'deja' pasar).","palabras_clave":"IRRUPCION|ENVOIS|HOSPITALIDAD|TARJETAPOSTALIZACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1212,"idioma_id":4,"titulo":"Use of electrical nerve stimulation to monitor lumbosacral epidural needle placement in cats","resumen":"Objective To determine the minimal electrical threshold (MET) necessary to elicit muscle contrac- tion of the pelvic limb or tail when an insulated needle is positioned outside (METout) and inside (METin) the lumbosacral epidural space in cats.Study design Prospective, blinded study.Animals Twelve mixed-breed healthy adult cats, scheduled for a therapeutic procedure where lum- bosacral epidural administration was indicated.Methods Under general anesthesia, an insulated needle was advanced through tissues of the lumbo- sacral interspace until its tip was thought to be just dorsal to the interarcuate ligament. An increasing electrical current (0.1 ms, 2 Hz) was applied through the stimulating needle in order to determine the MET necessary to obtain a muscle contraction of the pelvic limb or tail (METout), and then 0.05 mL kg1 of iohexol was injected. The needle was further advanced until its tip was thought to be in the epidural space. The MET was determined again (METin) and 0.2 mL kg1 of iohexol was injected. The cats were maintained in sternal position. Con- trast medium spread was determined through lateral radiographic projections.Results The radiographic study confirmed the cor- rect needle placement dorsal to the interarcuate AbstractObjective To determine the minimal electrical threshold (MET) necessary to elicit muscle contrac- tion of the pelvic limb or tail when an insulated needle is positioned outside (METout) and inside (METin) the lumbosacral epidural space in cats.Study design Prospective, blinded study.Animals Twelve mixed-breed healthy adult cats, scheduled for a therapeutic procedure where lum- bosacral epidural administration was indicated.Methods Under general anesthesia, an insulated needle was advanced through tissues of the lumbo- sacral interspace until its tip was thought to be just dorsal to the interarcuate ligament. An increasing electrical current (0.1 ms, 2 Hz) was applied through the stimulating needle in order to determine the MET necessary to obtain a muscle contraction of the pelvic limb or tail (METout), and then 0.05 mL kg1 of iohexol was injected. The needle was further advanced until its tip was thought to be in the epidural space. The MET was determined again (METin) and 0.2 mL kg1 of iohexol was injected. The cats were maintained in sternal position. Con- trast medium spread was determined through lateral radiographic projections.Results The radiographic study confirmed the cor- rect needle placement dorsal to the interarcuateligament in all cats. When the needle was placed ventrally to the interarcuate ligament, iohexol was injected epidurally in ten and intrathecally in two cats. The METout and METin was 1.76 ` 0.34 mA and 0.34 ` 0.07 mA, respectively (p < 0.0001).Conclusion and clinical relevance Nerve stimula- tion can be employed as a tool to determine penetra- tion of the interarcuate ligament but not the piercing of the dura mater at the lumbosacral space in cats.Keywords cat, electrical threshold, epidural, intra- thecal, nerve stimulation.","palabras_clave":"cat|epidural| electrical threshold|nerve stimulation."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1213,"idioma_id":4,"titulo":"Mössbauer and vibrational DOS studies of diluted magnetic tin oxides and nano iron oxides","resumen":"The magnetic properties and Mössbauer results of SnO2 doped with 57Fe  are reviewed, and the values of isomer shift and quadrupole splitting are compared 2 with the results by ab initio calculations. It is concluded that the exchange interactions between oxygen defects and magnetic atoms are responsible for long range magnetic interactions of dilute Fe ions dispersed in SnO2. Fe atom precipitated clusters may be formed in highly Fe doped SnO2 by annealing at relatively high temperatures for several hours. The reduction of the particle size to nano-scale dimensions induces magnetization, which can be associated with oxygen defects. We have measured the nuclear inelastic scattering of Fe oxides and 57Fe and (Co or Mn) doped SnO2 synthesized mainly by sol?gel methods and we have derived the vibration density of states (VDOS). The local phonons are sensitive to the presence of precipitated clusters.","palabras_clave":"ab initio|mosbauer|oxides|impurities"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1216,"idioma_id":4,"titulo":"Inorganic mercury (Hg2+) uptake by different plankton fractions of Andean Patagonian lakes (Argentina)","resumen":"The species composition and the size structure of natural planktonic food webs may provide essential information to understand the fate of mercury and, in particular, the bioaccumulation pattern of Hg2+ in the water column of lake ecosystems. Heterotrophic and autotrophic picoplankton and phytoplankton are the most important entry points for Hg in aquatic ecosystems since they concentrate Hg2+ and MeHg from ambient water, making them available to planktonic consumers at higher trophic levels of lake food webs. In this investigation we studied theuptake of 197Hg2+ in natural plankton assemblages from four Andean lakes (Nahuel Huapi National Park, Patagonia, Argentina), comprised in the size fractions 0.2-2.7 µm (picoplankton), 0.2-20 µm (pico and nanoplankton) and 20-50 µm  microplankton) through experiments using Hg2+ labeled with 197Hg2+. The experimental results showed that the uptake of Hg2+ was highest in the smallest plankton fractions (0.2-2.7 µm and 0.2-20 µm) compared to the larger fraction comprising microplankton (20-50 um). This pattern was consistent in all lakes, reinforcing the idea that among pelagic organisms, heterotrophic and autotrophic bacteria with the contribution of nanoflagellates and dinoflagellates constitute the main entry point of Hg2+ to the pelagic food web. Moreover, a significant direct relationship was found between the Hg2+ uptake and surface index of the planktonic fractions (SIf). Thus, the smaller planktonic fractions which bore the higher SI were the major contributors to the Hg2+ passing from the abiotic to the biotic pelagic compartments of these Andean lakes.","palabras_clave":"MERCURY|BIOACCUMULATION|PLANKTON SIZE FRACTIONS|ANDEAN PATAGONIAN LAKES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1217,"idioma_id":4,"titulo":"Evaluation of the Catalytic Activity of H3PW12O40 in the Selective Oxidation of Sulfides to the Corresponding Sulfoxides or Sulfones","resumen":"H3PW12O40 in bulk form presents suitable properties to be used as catalyst in the selective oxidation of sulfides  with 35% aqueous hydrogen peroxide, and sulfoxides or sulfones were obtained as products in high to excellent yields and  selectivity. We found that sulfides were oxidized to the corresponding sulfoxides, with near stoichiometric 35% w\/V  aqueous hydrogen peroxide, at room temperature (20ºC) and with a catalytic amount of bulk H3PW12O40. Similarly, sulfides  were transformed to the corresponding sulfones using excess of 35% w\/V aqueous hydrogen peroxide and a temperature  of 70ºC. In both cases acetonitrile was used as reaction solvent.","palabras_clave":"Aqueous hydrogen peroxide|Keggin heteropolyacid|sulfide oxidation|sulfoxides|sulfones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1218,"idioma_id":4,"titulo":"Chitosan microparticles: influence of the gelation process on the release profile and oral bioavailability of albendazole, a class II compound","resumen":"Encapsulation of albendazole, a class II compound, into polymeric microparticles based on chitosan-sodium lauryl sulfate was investigated as a strategy to improve drug dissolution and oral bioavailability. The microparticles were prepared by spray drying technique and further characterized by means of X-ray powder diffractometry, infrared spectroscopy and scanning electron microscopy. The formation of a novel polymeric structure between chitosan and sodium lauryl sulfate, after the internal or external gelation process, was observed by infrared spectroscopy. The efficiency of encapsulation was found to be between 60 and 85% depending on the internal or external gelation process. Almost spherically spray dried  microparticles were observed using scanning electron microscopy. In vitro dissolution results indicated that the microparticles prepared by internal gelation released 8% of the drug within 30 min, while the microparticles prepared by external gelation released 67% within 30 min. It was observed that the AUC values of ABZ from microparticles were greatly improved, in comparison with the non-encapsulated drug. In conclusion, the release properties and oral bioavailability of albendazole were greatly improved by using spraydried chitosan-sodium lauryl sulphate microparticles. In vitro dissolution results indicated that the microparticles prepared by internal gelation released 8% of the drug within 30 min, while the microparticles prepared by external gelation released 67% within 30 min. It was observed that the values of ABZ from microparticles were greatly improved, in comparison with the non-encapsulated drug. In conclusion, the release properties and oral bioavailability of albendazole were greatly improved by using spraydried chitosan-sodium lauryl sulphate microparticles.In vitro dissolution results indicated that the microparticles prepared by internal gelation released 8% of the drug within 30 min, while the microparticles prepared by external gelation released 67% within 30 min. It was observed that the AUC and CMAX values of ABZ from microparticles were greatly improved, in comparison with the non-encapsulated drug. In conclusion, the release properties and oral bioavailability of albendazole were greatly improved by using spraydried chitosan-sodium lauryl sulphate microparticles.","palabras_clave":"ALBENDAZOLE|MICROPARTICLES|ENCAPSULATION|BIOAVAILABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1220,"idioma_id":4,"titulo":"Senescence and yield responses to plant density in stay green and earlier-senescing maize hybrids from Argentina","resumen":"NA","palabras_clave":"MAIZE|ZEA MAYS|STAY GREEN|SENESCENCE|PLANT DENSITY|NITROGEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1221,"idioma_id":4,"titulo":"Effects of Equid herpesvirus 1 (EHV-1) AR8 and HH1 strains on BALB-c mice","resumen":"&nbsp;Here, we used a murine model to describe and compare the pathogenic potential of the Argentinean equid herpesvirus 1 (EHV-1) AR8 strain with the Japanese HH1 reference strain. In AR8-inoculated animals, clinical signs began&nbsp; earlier,&nbsp; but&nbsp; the&nbsp; viremic&nbsp; phase&nbsp; was&nbsp; shorter.&nbsp; Virus isolation and DNA detection in the lungs, liver and spleen were positive for both strains at different times postinfection&nbsp; (pi).&nbsp; Infection&nbsp; foci&nbsp; produced&nbsp; by&nbsp; both&nbsp; strains&nbsp; were&nbsp; immunohistochemically&nbsp; detected&nbsp; in&nbsp; lungs&nbsp; from&nbsp; day&nbsp; 1&nbsp; to day&nbsp; 4&nbsp; pi.&nbsp; We&nbsp; conclude&nbsp; that&nbsp; whichever&nbsp; EHV-1&nbsp; strain&nbsp; is selected to experimentally reproduce the disease, it needs appropriate&nbsp;&nbsp; standardization&nbsp;&nbsp; in&nbsp;&nbsp; order&nbsp;&nbsp; to&nbsp;&nbsp; provide&nbsp;&nbsp; valid conclusions.","palabras_clave":"EHV-1 AR8 strain|EHV-1 HH1 strain|pathogenicity|mice"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1222,"idioma_id":4,"titulo":"Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation","resumen":"The maternal-fetal interface is a unique immunological site that generates an adequate microenvironment during pregnancy, recognizing and eliminating infections and tolerating the trophoblast\/placenta unit. For that purpose, trophoblast cells display several tolerogenic mechanisms to allow fetal survival, such as production of the neuropeptide vasoactive intestinal peptide (VIP). Here we investigated the contribution of VIP to maintain homeostasis at the maternal-placental interface under lipopolysaccharide (LPS) stimulation.  METHODS:  We performed cocultures between trophoblast cells (Swan-71 cell line) and maternal leukocytes obtained from fertile women as an in vitro model of maternal-placental interaction, and we focused on the effects of LPS on the modulation of VIP and their receptors (VPAC1 and VPAC2).  RESULTS:  VIP could prevent the upregulation of IL-6, MCP-1, and nitrite production and maintain the production of IL-10 and TGF-&#946; under LPS (10 µg\/ml) stimulation after 48 h of coculture. To gain deeper insight into the mechanisms of how VIP could contribute to a tolerogenic microenvironment even in the presence of LPS, we investigated VIP production by maternal leukocytes and observed a significant increase in the frequency of CD4+VIP+ cells after interaction with Swan-71 cells in the presence of LPS. LPS increased VIP and inducible receptor VPAC2 expression directly on trophoblast cells in a dose- and time-dependent manner.  CONCLUSIONS:  The present results suggest that VIP might act as an additional homeostatic mechanism during early stages at the maternal-placental interface to control exacerbated inflammatory responses such as the ones observed in intrauterine infections.","palabras_clave":"VIP|LPS|IMMUNE HOMEOSTASIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1223,"idioma_id":4,"titulo":"Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice","resumen":"Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies were detected in a non-obese diabetic (NOD) mouse model of type I insulin-dependent diabetes mellitus and Sjögren´s syndrome, two comorbid states with CD. Since pregnancy complications have been described in NOD mice, we evaluated the ability of anti-tTG antibodies to affect the functions of tTG relevant to the normal course of an early pregnancy like extracellular matrix assembling and apoptotic cell phagocytosis by macrophages. Circulating IgG antibodies against tTG were detected in NOD mice with titers that decreased at early pregnancy; interestingly, the in vitro transamidating activity of tTG was reduced by NOD serum samples. Particularly, anti-tTG antibody inhibited apoptotic cell phagocytosis by peritoneal macrophages from pregnant NOD mice that express the enzyme on surface. Evidence provided support for a role for anti-tTG antibodies through reduced transamidating activity and reduced apoptotic cell clearance by the macrophages of pregnant NOD mice.","palabras_clave":"Anti-tissue transglutaminase antibodies|Celiac Disease|NOD mice"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1224,"idioma_id":4,"titulo":"Symbiotic seed germination and protocorm development of Aa achalensis Schltr., a terrestrial orchid endemic from Argentina","resumen":"Aa achalensis is an endangered terrestrial orchid endemic from Argentina. In vitro symbiotic seed germination was evaluated for its propagation. Five different fungal strains were isolated from this species: two Rhizoctonia-like related to Thanatephorus cucumeris and three ascomicetaceous fungi belonging to Phialophora graminicola and one to an uncultured Pezizaceae. All five isolates promoted seed germination being one T. cucumeris strain the most effective. After 16 weeks of growth, 30 % of A. achalensis protocorms developed until seedlings with two\/four leaves in this treatment. These findings open an opportunity to the knowledge and preservation of this species.","palabras_clave":"ORCHID MYCORRHIZA |SEED GERMINATION|DARK SEPTATE ENDOPHYTES|CONSERVATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1226,"idioma_id":4,"titulo":"A revision of Old World species of Setaria (Poaceae: Panicoideae: Paniceae)","resumen":"Setaria (Poaceae: Panicoideae: Paniceae) in the Old World is revised, providing necessary identification tools for a group that includes many important forage grasses and several widespread weeds. Sixty six species are treated in this work, in which macromorphological characters are considered. Setaria includes annual and perennial species of open and dry places, with membranous-ciliate ligules, leaves lanceolate to filiform, inflorescences lax to spiciform, spikelets solitary or paired and with one to several setae below; spikelets obovoid to long ellipsoid, glabrous, with the lower glume usually 1\/2 or less the length of the spikelet, upper glume and lower lemma subequal, lower palea as long as the upper anthecium to reduced or absent, lower flower absent or present, when present staminate, upper anthecium indurate, smooth to transversely rugose, and a basic chromosome number of x = 9. A key to the species, synonymy, typifications, morphological descriptions of the 65 species, notes, and illustrations are presented.","palabras_clave":"SETARIA|OLD WORLD|TAXONOMY|POACEAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1227,"idioma_id":4,"titulo":"On the taxonomic position of Panicum scabridum (Poaceae, Panicoideae, Paspaleae)","resumen":"Panicum scabridum, an incertae sedis species of Panicum s.l., is here included in the genus Coleataenia, following aphylogenetic analysis based on one new ndhF sequence of the species and associated morphological data. Panicumscabridum and species of Coleataenia are cespitose and perennial plants, with a lower glume (1?)3?5-nerved, 1\/3 to 3\/4of the spikelet, upper glume and lower lemma 5?9-nerved, and upper anthecium smooth, shiny, and indurate. WithinColeataenia, P. scabridum appeared as the sister taxon of the species pair C. prionitis and C. petersonii; these threespecies are the only NADP-me taxa of tribe Paspaleae exhibiting two bundle sheaths around the vascular bundles, i.e.,with an outer parenchymatous sheath and an inner mestome sheath with specialized chloroplasts. The new combinationColeataenia scabrida is proposed and a lectotype is designated.","palabras_clave":"PANICUM SCABRIDUM|PHYLOGENY|COMBINED ANALYSIS|ANATOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1228,"idioma_id":4,"titulo":"Grazing impact on desert plants and soil seed banks: implications for seed-eating animals","resumen":"We assess whether the knowledge of livestock diet helps to link grazing effects with changes in plant cover and soil seed bank size, aiming at inferring the consequences of grazing on seed-eating animals. Specifically, we test whether continuous and heavy grazing reduce the cover, number of reproductive structures and seed reserves of the same grass species whose seeds are selected and preferred by granivorous animals in the central Monte desert, Argentina. Grass cover and the number of grass spikes usually diminished under grazing conditions in the two localities studied (Telteca and Ñacuñán), and soil seed bank was consistently reduced in all three years evaluated owing to a decline of perennial grass and forb seeds. In particular, the abundance of those seeds selected and preferred by birds and ants (in all cases grass species) declined 70e92% in Ñacuñán, and 52e72% in Telteca. Reduction of perennial grass cover and spike number in grazed sites reinforced the causal link between livestock grazing and the decline of grass soil seed reserves throughout failed plant reproduction. Grass seed bank depletion suggests that grazing may trigger a ?cascade? of mechanisms that affect the abundance and persistence of valuable fodder species as well as the availability of seed resources for granivorous animals.","palabras_clave":"CASCADE CAUSALITY|DISTURBANCE ECOLOGY|LIVESTOCK DIET|SEED-EATING ANIMALS DIET"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1229,"idioma_id":4,"titulo":"Adaptación y validación en población española de la Escala de Expectativa de los hijos adolescentes sobre la reacción de sus padres frente al comportamiento prosocial y antisocial.","resumen":"El objetivo de esta investigación es presentar una adaptaciòn y validación en población española de la Escala de expectativa de los hijos adolescentes sobre la reacción de sus padres frente al comportamiento prosocial y antisocial,desarrollado por Wyatt y Carlo (2002). La muestra estuvo compuesta por 631 adolescentes españoles de entre 15 y 18 años de edad,de ambos sexos, de clase media. Se realizó un análisis factorial confirmatorio para estudiar la validez de constructo de la escala y se analizó la consistencia interna a través de del cálculo de alpha de Cronbach.Los resultados indican que la escala posee buenos índices de ajuste al modelo teórico propuesto por los autores en su versión original.Asimismo, se realizo un análisis multigrupo con la intención de mostrar la invarianza del modelo en función del sexo.El modelo se mantuvo invariante,lo que confirma la estructura bifactorial de la escala que mide la expectativa de los adolescentes de la reacción de sus padres frente a las conductas adaptativas y desadaptativas.","palabras_clave":"EXPECTATIVA PARENTAL|CONDUCTA PROSOCIAL|CONDUCTA ANTISOCIAL|ADOLESCENCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1231,"idioma_id":4,"titulo":"Livestock stations as foci of groundwater recharge and nitrate leaching in a sandy desert of the Central Monte, Argentina","resumen":"In arid ecosystems, evapotranspiration generally exceeds precipitation, preventing deep drainage and groundwater recharge. We propose that vegetation changes associated with the establishment of pastoralist settlements (i.e. livestock stations) can disrupt the ecological and hydrological linkages in arid groundwater-coupled ecosystems of the Monte desert (Argentina), allowing local groundwater recharge and nitrate leaching to the aquifer, affecting groundwater quality. We tested this hypothesis by analysing vegetation, land use indicators, water and nitrate dynamics in three pairs of livestock stations and relatively undisturbed control woodlands. Livestock stations had lower vegetation and dead wood but higher dung covers than control woodlands, indicating soil and vegetation changes associated to land use. Water and nitrate dynamics were also affected by land use. Soil nitrate and water contents sampled down to the water table were higher, and soil chloride and salinity were lower in livestock stations, indicating higher water percolation and N input\/transport rates. Higher groundwater nitrate concentrations in livestock stations indicate that these areas behave as foci of N and water export from ecosystems to the phreatic aquifer. Our study supports the idea that vegetation in arid areas prevents downward surface?groundwater interactions, but it also indicates that human modifications of vegetation disrupt this control, reducing soil water consumption and allowing vertical movement of water and solutes to the aquifer, which can modify groundwater quality. Disruptions of ecological processes by livestock activities clearly affect the hydrological links between surface and groundwater.","palabras_clave":"FREÁTICA|LAND USE CHANGE|SOIL PROFILE|GRAZING SYSTEMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1232,"idioma_id":4,"titulo":"Effects of AR8 and HH1 Equid herpesvirus 1 (EHV-1) strains on BALB-c mice","resumen":"The pathogenic potential of the Argentinean Equid herpesvirus 1 (EHV-1) AR8-strain isdescribed for the first time and compared with the reference virulent Japanese HH1strain in mice at different intranasal post-inoculation (pi) times. AR8-inoculated animalsshowed dyspnea and ruffled fur from day 1 pi, whereas in those inoculated with HH1clinical signs began later. Viremic phase was shorter in AR8-inoculated mice. Virusisolation and DNA detection from lung, liver and spleen were positive for each strain atdifferent pi times. Infection foci were only immunohistochemically detected in bronchialand bronchiolar epithelium from day 1 to 4 pi for each strain. Our results using HH1differed from those previously reported. The relevance of standardization of any EHV-1strain before providing valid conclusions is highlighted. We suggest and support theuse of native EHV-1 strains to better understand the disease process.","palabras_clave":"EHV-1 AR8 STRAIN|EHV-1 HH1 STRAIN|PATHOGENICITY|VIREMIA|MICE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1233,"idioma_id":5,"titulo":"Vasculatura portal intrahepatica: descripcion anatomica, radiologica  y ecografica","resumen":"El presente trabajo describe la arborización del sistema venoso portal intrahepático desde los enfoques anatómico, radiológico y ecográfico. Se realizaron 5 preparados anatómicos mediante inyección de resinas plásticas y posterior corrosión. Por radiolo- gía se contrastó el árbol portal en una pieza anatómica. Se realizaron estudios ecográficos bidimensionales y Doppler en 13 caninos de raza Beagle. Estos procedimientos permitieron a los autores comparar la tridimensionalidad de los preparados anatómicos con la bidimensionalidad de los estudios por imágenes.","palabras_clave":"Canino| Circulación portal|Portografía|Doppler"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1234,"idioma_id":4,"titulo":"An inverse latitudinal biodiversity pattern in asellote isopods (Crustacea, Peracarida) from the Southwest Atlantic between 35° and 56°S","resumen":"A distinct trend of decreasing biodiversity from the tropics to the poles is well-known for terrestrial organisms. This pattern, however, is less clear in marine systems. In the present study, an inverse latitudinal biodiversity pattern is reported for the asellote isopods from Argentina. Species richness is shown to be about six times higher in the Beagle Channel and southern Patagonia, i.e., south of 47°S, than north of this latitude. This high species richness of Asellota south of 47°S seems to be related with the predominance of gravelly bottoms in the southern Patagonian shelf and also with the tectonic history of the southern tip of South America. Inverse latitudinal gradients had been reported previously for echinoderms, bryozoans, sponges, amphipods and macroalgae from the southern Southwest Atlantic. Based on unpublished new records and information gathered from the literature, a database summarizing the distribution ranges of the Asellota along the coast of Argentina was compiled. A total of 108 species was recorded and the distribution records of this fauna were increased by 36.15 % (260 and 354 before and after our surveys, respectively).","palabras_clave":"ASELLOTA|ISOPODA|SPECIES RICHNESS|LATITUDINAL BIODIVERSITY PATTERN|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1235,"idioma_id":4,"titulo":"Shoot growth and development of Berberis buxifolia Lam. in Tierra del Fuego (Patagonia)","resumen":"Knowledge on the annual cycle of growth and development of  several fruit species from temperate climate is available; however, few  reports exist on fruit shrubs from cold climates like those of Berberis  genus. The objective of this work was to study the shoot growth and  development of the native Patagonian species B. buxifolia Lam, whose  black blue fruits are of economic value. Data were recorded from adult  plants that were planted in a representative area located near Ushuaia  city, 54º 48´SL, 68º 19´WL, (Tierra del Fuego, Argentina). Shoots grown  during the growing season developed vegetative and mixed buds in their  nodes. Vegetative buds differentiated numerous leaves distributed around  the central axis with the point of insertion nearly to the same height.  In effect, when the bud sprouting starts, a rosette-like growth occurs.  Flower differentiation started twelve weeks after bud breaks in  coincidence with the end of the first fruit growth phase. The following  growing season, thirteen weeks later of sprouting, stamen primordial and  a single carpel could be shown. Gamete differentiation occurred in a  short period before sprouting for male gametes and during flowering time  for female gametes. Blooming period occurred on spring and the length of  the anthesis phase was closely related to the climatic conditions. While  fruit growth followed a double sigmoid curve, shoot elongation showed a  sigmoid curve. Knowledge of shoot growth and development of B. buxifolia  is of great importance to understand its physiology, and when culture  practices need to be incorporated like plant fertilization or when  evaluating the impact of adverse climatic conditions on events as  flowering and fruit set.","palabras_clave":"Vegetative growth|floral differentiation|annual cycle|barberry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1236,"idioma_id":5,"titulo":"En busca de un orden jerárquico: los intentos de catolización de la sociedad argentina en la década de 1930.","resumen":"Tras el golpe de Estado de 1930 la Iglesia Católica Argentina buscó impregnar sus idearios y prácticas en los sectores dirigentes de la sociedad y, desde allí, alcanzar el dominio del conjunto social para instaurar la nación católica. El catolicismo debía salir del espacio privado de las conciencias para transformarse en actor político fundador de  un nuevo orden social","palabras_clave":"catolicismo|política|intelectuales|sociedad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1237,"idioma_id":4,"titulo":"SBVR to OWL 2 Mappings - An Automatable and Structural-Rooted Approach","resumen":"The wide applicability of mapping business rules expressions to ontology statements have been recently recognized. Some of the most important applications are: (1) using of ontology reasoners to prove the consistency of business domain information, (2) generation of an ontology intended to be used in the analysis stage of a software development process, and (3) the possibility of encapsulate the declarative specication of business knowledge into information software systems by  means of an implemented ontology. The Semantics of Business Vocabulary and Business Rules (SBVR) supports that approach by providing business people with a linguistic way to semantically describe business concepts and specify business rules in an independent way of any information system design. Although previous work have presented some proposals, an exhaustive and automatable approach for them is still lacking. This work presents a broad and detailed set of transformations that allows the automatable generation of an ontology implemented in OWL 2 from the SBVR specications of a business domain. Such transformations are rooted on the structural specication of both standards and are depicted through a case study. A real case validation example was performed, approaching the feasibility of the mappings by the quality assessment of the developed ontology.","palabras_clave":"BUSINESS RULE|ONTOLOGY|MAPPING|SBVR|OWL 2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1238,"idioma_id":4,"titulo":"Bluetongue virus in South America, Central America and the Caribbean.","resumen":"It is well known that Bluetongue disease is spread all over the world but there are substantial differences between data available from different parts of the world. Some regions are not covered by proper surveillance programs and thus, we do not know the real situation concerning the disease in those areas. That is the case of Central America, South America and Caribbean, where few outdated data is reported about the presence and spread of BTV. Here we updated the known situation compiling serologic information available from the region and analyzed genetic information resulted from research groups which are studying the disease in the region.","palabras_clave":"BLUETONGUE VIRUS|SOUTH AMERICA|CENTRAL AMERICA|EPIDEMIOLOGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1239,"idioma_id":4,"titulo":"High pressure phase behavior modeling of asymmetric Alkane + Alkane binary systems with the RKPR EOS","resumen":"Very asymmetric hydrocarbon mixtures present an interesting academic challenge for their modeling. Inaddition, they are becoming more important technologically, since the world oil reserves are becomingheavier in average.The RKPR EOS was developed in recent years in order to consider asymmetric systems and allowfor a good representation of densities for different types of compounds while maintaining the relativesimplicity of cubic equations of state. In this work the RKPR EOS is applied for the first time for thecorrelation of phase behavior in the series of binary systems methane + n-alkane and ethane + n-alkane. Inaddition to the use of attractive and repulsive interaction parameters with quadratic mixing rules, the roleof the extra degree of freedom that the RKPR model offers for setting the pure compound parameters wasalso examined. A new table of RKPR pure compound parameters was defined for n-alkanes. A comparativestudy was made with the Peng?Robinson EOS, based on following the same optimization procedure forinteraction parameters.","palabras_clave":"Methane|Ethane|Hydrocarbons|High pressure phase behavior|RKPRa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1240,"idioma_id":4,"titulo":"Magnetizabilities of Diatomic and Linear Triatomic Molecules in a Time-Independent Nonuniform Magnetic Field","resumen":"The theory of response of a molecule in the presence of a static non-uniform magnetic field with uniform gradient is reviewed and extended. Induced magnetic dipole, quadrupole and anapole moments are expressed via multipole magnetic susceptibilities. Dependence of response properties on the origin of the coordinate system with respect to which they are defined is investigated. Relationships describing the change of multipole and anapole susceptibilities in a translation of the reference system are reported. For a single molecule, and more generally in an ordered medium, two quantities are invariant and, in principle, experimentally measurable, i.e., the induced magnetic dipole and the interaction energy. The trace of a second-rank anapole susceptibility, related to a pseudoscalar obtained by spatial averaging of the dipole-quadrupole susceptibility, of different sign for D and L enantiomeric systems, is origin independent. Therefore, in an isotropic chiral medium, a homogeneous magnetic field induces an electronic anapole, having the same magnitude but opposite sign for two enantiomorphs. Calculations have been carried out for a set of diatomic and linear triatomic systems characterized by the presence of magnetic-field induced toroidal electron currents.","palabras_clave":"magnetizability|magnetic anapole|linear molecules|enantiomeric recognition"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1242,"idioma_id":4,"titulo":"INFLUENCE OF THE MARTENSITE VOLUME FRACTION ON THE RECIPROCATING SLIDING BEHAVIOUR OF Ti-Nb MICROALLOYED STEEL","resumen":"The objective of this work was to evaluate the in&#64258;uence of martensite fraction on the wear mode and the energy dissipation by friction of dual phase (DP) steel tested under reciprocating sliding conditions. For this purpose, a Ti-Nb microalloyed steel was heat treated in a conventional furnace at temperatures between 780 and 880&#9702;C (intercritical annealing temperature) for 3 min to obtain DP microstructures with volume fractions of martensite between 25 and 90%. Wear tests were carried out in both DP and as-received samples, using a reciprocating tribometer with ball-on-&#64258;at geometry, at two constant applied loads, 2.5 and 4 N. The wear damage of each sample was measured through volume loss and the dissipated energy during the test. The obtained results evidenced a signi&#64257;cant in&#64258;uence of the contact load over the wear mode, because at low load the DP wear was reduced with increased hardness but just up to 75% of martensite. At high load, the sliding process promotes an oxide mixture in the ferritic microstructure that acts as a factor in wear reduction.","palabras_clave":"dual phase steel|sliding reciprocating|martensite|oxide"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1243,"idioma_id":4,"titulo":"Methodology developed for the simultaneous measurement of bone formation and bone resorption in rats based on the pharmacokinetics of fluoride","resumen":"This paper describes a novel methodology for the simultaneous estimation of bone formation (BF) and resorption (BR) in rats using fluoride as a nonradioactive bone seeker ion. Fluoride pharmacokinetic has been extensively studied in rats; its constants have all been characterized. This knowledge was the cornerstone for the underlying mathematical model we used to measure bone fluoride uptake and elimination rate after a dose of fluoride. Bone resorption and formation were estimated by bone fluoride uptake and elimination rate, respectively. ROC analysis showed that sensitivity, specificity and area under the ROC curve were not different from deoxypiridinoline and bone alkaline phosphatase, well known bone markers. Sprague Dawley rats with modified bone remodelling (ovariectomy, hyper and hypocalcic diet, antiresorptive treatment) were used to validate the values obtained with this methodology. The results of BF and BR obtained with this technique were as expected for each biological model. Although the method should be performed under general anaesthesia, it has several advantages: simultaneous measurement of BR and BF, low cost and the use of compounds with no expiration date.","palabras_clave":"BONE FORMATION|BONE RESORPTION|FLUORIDE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1244,"idioma_id":4,"titulo":"A Note on the Estimation of the Hölder Constant","resumen":"In this paper we develop a nonparametric estimator for the Holder constant of a densityfunction. We consider a simulation study to evaluate the performance of the proposal and construct smooth bootstrap confidence intervals. Also, we give a brief review over the impossibility to decide whether a density function is Holder.","palabras_clave":"NONPARAMETRIC ESTIMATION|HOLDER CONSTANT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1245,"idioma_id":4,"titulo":"Increased in vitro glial fibrillary acidic protein expression, telomerase activity, and telomere length after productive human immunodeficiency virus-1 infection in murine astrocytes.","resumen":"Abstract  Although HIV-associated neurocognitive disorders (HAND) result from injury and loss of neurons, productive infection routinely takes place in cells of macrophage lineage. In such a complex context, astrocytosis induced by local chemokines\/cytokines is one of the hallmarks of HIV neuropathology. Whether this sustained astrocyte activation is able to alter telomere-aging process is unknown. We hypothesized that interaction of HIV with astrocytes may impact astrocyte telomerase activity (TA) and telomere length in a scenario of astrocytic activation measured by expression of glial fibrillary acidic protein (GFAP). To test this hypothesis, cultured murine astrocytes were challenged with pseudotyped HIV\/vesicular stomatitis virus (HIV\/VSV) to circumvent the absence of viral receptors; and GFAP, telomerase activity, and telomere length were quantified. As an early and transient event after HIV infection, both TA activity and telomere length were significantly augmented (P&#8201;<&#8201;0.001). Later, a strong negative correlation (-0.8616, P&#8201;<&#8201;0.0001) between virus production and telomerase activity was demonstrated. Once HIV production had reached a peak (7 dpi), the TA decreased, showing levels similar to those of noninfected cells. In contrast, the astrocyte became activated, exhibiting significantly increased levels of GFAP expression directly related to the level of HIV\/VSV replication (P&#8201;<&#8201;0.0001). Our results suggest that HIV-infected astrocytes exhibit early disturbance in their cellular functions, such as telomerase activity and telomere length, that may attenuate cell proliferation and enhance the astrocyte dysregulation, contributing to HIV neuropathogenesis. Understanding the mechanisms involved in HIV-mediated persistence by altering the telomere-related aging processes could aid in the development of therapeutic modalities for neurological complications of HIV infection.","palabras_clave":";ASTROCYTES|TELOMERASE |TELOMERASE LENGTH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1247,"idioma_id":4,"titulo":"Cationic disorder and Mn3+\/Mn4+ charge ordering in the B&#8242; and B&#8243; sites of Ca3Mn2NbO9 perovskite: a comparison with Ca3Mn2WO9","resumen":"We describe the preparation, crystal structure determination, magnetic and transport properties of two novel Mn-containing perovskites, with a different electronic configuration for Mn atoms located in B site. Ca3Mn3+2WO9 and Ca3Mn3+\/4+2NbO9 were synthesized by standard ceramic procedures; the crystallographic structure was studied from X-ray powder diffraction (XRPD) and neutron powder diffraction (NPD). Both phases exhibit a monoclinic symmetry (S.G.: P21\/n); Ca3Mn2WO9 presents a long-range ordering over the B sites, whereas Ca3Mn2NbO9 is strongly disordered. By ?in-situ? NPD, the temperature evolution of the structure study presents an interesting evolution in the octahedral size (&#12296;Mn?O&#12297;) for Ca3Mn2NbO9, driven by a charge ordering effect between Mn3+ and Mn4+ atoms, related to the anomaly observed in the transport measurements at T&#8776;160&nbsp;K. Both materials present a magnetic order below TC=30&nbsp;K and 40&nbsp;K for W and Nb materials, respectively. The magneto-transport measurements display non-negligible magnetoresistance properties in the paramagnetic regime.","palabras_clave":"Double perovskite|Crystal structure|Magnetic behaviour|Transport properties|Charge ordering"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1248,"idioma_id":4,"titulo":"Microstructure and texture analysis of the &#948;-hydride precipitation in Zircaloy-4 materials by electron microscopy and neutron diffraction","resumen":"This work presents a detailed microstructure and texture study of various hydrided Zircaloy-4 materials by neutron diffraction and microscopy. The results show that the precipitated &#948;-ZrH1.66 generally follows the &#948;(111)\/\/&#945;(0001) and &#948;[]\/\/&#945;[] orientation relationship with the &#945;-Zr matrix. The &#948;-hydride displays a weak texture that is determined by the texture of the &#945;-Zr matrix, and this dependence essentially originates from the observed orientation correlation between &#945;-Zr and &#948;-hydride. Neutron diffraction line profile analysis and high-resolution transmission electron microscopy observations reveal a significant number of dislocations present in the &#948;-hydride, with an estimated average density one order of magnitude higher than that in the &#945;-Zr matrix, which contributes to the accommodation of the substantial misfit strains associated with hydride precipitation in the &#945;-Zr matrix. The present observations provide an insight into the behaviour of &#948;-hydride precipitation in zirconium alloys and may help with understanding the induced embrittling effect of hydrides.","palabras_clave":"hydrides|zirconium|neutron diffraction|titanium hydride"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1249,"idioma_id":4,"titulo":"Effects of sex and season in haematological parameters and cellular composition of spleen and head kidney of pejerrey (Odontesthes bonariensis)","resumen":"Phylognetic diversity in fish determines high interspecific variability in morphgology as well as in phisiological parameters.Moreover ,several haematological variables and the organ composition of haemolymphopoietic sites may very according to sex or season.The aim of this study was to establish the&nbsp;haematological parameters and de cellular composition of haemolymphopoietic organs in 'Odontesthes bonariensis' a comercially valuable fish species in Argentina, and also to determine gender or seasonal variations .Haematocrit exhibited the hihest value in summer, with haemoglobin concentration was greater in summer and automn","palabras_clave":"haematology|blood cell lineages|seasonal variations|fish"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1250,"idioma_id":4,"titulo":"Seed longevity and fire: post-germination responses of an exotic herb in NW Patagonian grasslands (Argentina)","resumen":"The cosmopolitan herb Rumex acetosella forms persistent soil seed banks and increasesin cover after fire. We investigated how the interaction between seed age and fire affectsseedling growth by exposing different-aged seeds to heat, smoke, charcoal, and ashtreatments. We measured growth of germinated seedlings that were transplanted andallowed to grow for 65 days in a greenhouse. Seedlings from seeds >8 years old did notreach an appropriate radicle length for transplantation. Seedling growth decreased withincreasing temperature of the heat treatment. As seed age increased, growth decreasedwith smoke and charcoal, and increased with ash treatment. Height was negativelycorrelated with seed age. Our results suggest that fire and seed age could affect demographicresponses of R. acetosella seedling populations. Post-fire recruitment could bepartially favored by the positive effect of nutrient input from ash on seedling growth.High fire intensities, however, would be detrimental to seedling vigor.","palabras_clave":"Fire|Seeds|Rumex|Grasslands"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1251,"idioma_id":4,"titulo":"Inferiorisation and deference: the processes of constructing social hierarchies in the context of paid domestic labour","resumen":"Revista indexada en Scopus \/ Sociological Abstracts\/ ASSIA \/ Alt Press IND \/ British Humanities Index \/ Feminist Periodicals \/ Research Alert entre otros directorios.          Los nombres de los autores aparecen en la publicación según el orden alfabético de sus apellidos.                  In  Argentina, domestic service is one of the main occupations for women  from low-income sectors. As in other Latin American societies, it is  one of the most paradigmatic forms of contact between the different  social classes. As such, this labour relationship has been analysed  in numerous studies as a critical location for the reproduction of  social differences and inequality. The interpersonal relationships  between employers and workers mobilise categorisation criteria and  stereotyped images that reveal wider dynamics regarding the  construction of social hierarchies. On the basis of a qualitative  study, the objective of this article is to analyse, in the city of  Buenos Aires, the processes of constructing social hierarchies that  are implied by this particular labour relationship. This analysis  seeks to reveal the operations through which employers construct a  stereotype of social inferiority for domestic workers through which  they legitimise their dominant position in the labour relationship,  and to examine the tensions and ambiguities of this.","palabras_clave":"TRABAJADORAS DOMESTICAS|SERVICIO DOMESTICO|DESIGUALDAD SOCIAL|JERARQUIAS SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1252,"idioma_id":4,"titulo":"Kinetic and mechanistic behavior of the ascorbate ion-glutathione mixture in the  visible-light-mediated oxidation of tryptophan","resumen":"Within the framework of the frequently invoked correlation between antioxidant contents and  potential antioxidative protection, the stability of the amino acid (AA), tryptophan (trp), taken as a prototype of relevant photooxidizable biological target, was evaluated in the presence of the  known antioxidant ascorbate ion (AsA). The peptide  glutathione (GSH) was also included in order to eliminate thermal decomposition of AsA. Riboflavin (Rf, vitamin B2), endogenous in most biological environments, was employed as a photosensitizer. A systematic kinetic and mechanistic study was conducted under aerobic conditions in pH 7 aqueous medium. The evaluation of oxygen uptake rates (OUR) in the photoirradiated mixtures, taken as an overall indicator of the total oxidation degree of the mixture components, constitutes an interesting and useful tool in this case. In the presence of Rf, the isolated AA and AsA undergo photodegradation under conditions currently found in natural media. The photodegradations occur in a non-simple reaction, mediated by reactive oxygen species (ROS) such as singlet molecular oxygen (O2(1&#8710;g)), superoxide radical anion, and hydrogen peroxide and hydroxyl-radical. All ROS are photogenerated from triplet excited Rf after interaction with the ground state of AsA and trp. The photoirradiation also triggers an already described parallel thermally-driven and dark reaction on AsA which is stopped by the addition of GSH. The global outcome can be interpreted as a sort of conjunctive antioxidant protection exerted on the system AsA + trp + GSH upon Rf-photosensitization, a result that cannot be predicted from the individual behavior of the mixture components.","palabras_clave":"ASCORBATE ION|GLUTATHIONE|PHOTODEGRADATION|RIBOFLAVIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1254,"idioma_id":4,"titulo":"The minor inequalities in the description of the set covering polyhedron of circulant matrices","resumen":"verdoi : 10.1007\/s00186-013-0453-6","palabras_clave":"polyhedral combinatorics|circulant matrix|set covering"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1255,"idioma_id":4,"titulo":"Naming the living things: Linguistic, experiential and cultural factors in Wichí and Spanish speaking children","resumen":"This work focuses on the underlying conceptual structure of children?s category of living  things from a cross-cultural, cross-linguistic perspective. School-aged children (n=129) from three Argentinean communities (rural Wichí-speaking, rural Spanish-speaking, urban Spanish-speaking) were asked to generate the names of living things. Analyses were focused on the typicality, semantic organization, and hierarchical level of the names mentioned. We identified convergences among the names generated by children in all three communities, as well as key differences: The typicality, habitats and hierarchical level of the categories mentioned varied as a function of children?s language and their direct experience with the natural world. These findings provide evidence concerning the role of language, culture and experience in shaping children?s folkbiological concepts.","palabras_clave":"LIVING THING CONCEPT|FOLKBIOLOGY|SEMANTIC ORGANIZATION|CROSS-CULTURE|CROSS-LINGUISTIC|SPANISH|WICHI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1258,"idioma_id":5,"titulo":"Golpes de Estado en Argentina (1976-1983): Consecuencias Sociales, Culturales y psicológicas","resumen":"En Argentina, entre el 24 de marzo de 1976 y el 10 de diciembre de 1983, el poder político estuvo en manos de militares y civiles que tomaron el gobierno tras derrocar a un gobierno democrático. Siete años en el poder dejaron consecuencias en la sociedad, la economía y la cultura. Prueba de esto son los 30 000 desaparecidos, los 400 niños robados ?que a la fecha no han sido devueltos a sus familias?, los efectos en los excombatientes de la guerra de Malvinas, así como deterioros en las tramas subjetivas de las víctimas directas y sus familiares. Este ensayo intenta abarcar las consecuencias citadas, debido a que ?muchas de estas? aún mantienen perdurabilidad.","palabras_clave":"Dictadura|Desaparecidos|Consecuencias psicológicas|Cultura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1259,"idioma_id":4,"titulo":"Droxidopa for the treatment of neurogenic orthostatic hypotension and other symptoms of neurodegenerative disorders","resumen":"Introduction: L-threo-3,4-dihydroxyphenylserine  (droxidopa) is a pro-drug that is metabolized to norepinephrine (NE) in nerve  endings and in other tissues. It is commercialized in Japan since 1989 for treatment  of orthostatic hypotension symptoms in Parkinson´s disease (PD) with Hoehn  &amp; Yahr stage III, Shy-Drager syndrome, familial amyloid polyneuropathy; and  hemodialytic patients. It has also been recently approved by the FDA for symptomatic  neurogenic orthostatic hypotension.         Areas covered: In this review, clinical uses of  droxidopa in neurogenic orthostatic hypotension and other neurodegenerative  disorders will be reviewed. Results from a MEDLINE search with the keywords  ?droxidopa? OR ?L-threo-3,4-dihydroxyphenylserine? OR ?L-DOPS? will be  discussed. Abstracts from international congresses were also explored.        Expert opinion: Results from a few small and short  placebo-controlled trials clinical studies in neurogenic OH showed significant  reductions in manometric drop of blood pressure (BP) after posture changes or  meals. Larger Phase III studies suggest a positive effect of the drug on  dizziness, which has only been shown in the short-term (i.e. less than 2 weeks).  FDA approval was granted in the context of an ?accelerated approval program?,  which means that further studies have to be conducted in order to assess the long-term  effects of droxidopa on OH symptoms. Droxidopa has also been studied for the  treatment of other causes of OH, other symptoms of PD or other indications.  Nonetheless, the results of such pilot studies remain preliminary, as  randomized placebo-controlled clinical trials are generally lacking.","palabras_clave":"hipotension ortostática|droxidopa|enfermedad de parkinson|atrofia multisistémica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1260,"idioma_id":4,"titulo":"An  eco-friendly  strategy,  using  on-line  monitoring  and  dilution coupled  to  a  second-order  chemometric  method,  for  the  construction of  dissolution  curves  of  combined  pharmaceutical  associations","resumen":"A  simple,  precise,  economic  and  minimally  operator-dependent  method  was  developed  under  green analytical  chemistry  principles,  for  the  simultaneous  construction  of  the  dissolution  curves  of  a  pharmaceutical  association  in  short  time  and  without  employing  organic  solvents,  allowing  important  savings of  labor  and  resources.  The  carvedilol  (CAR)  and  hydrochlorothiazide  (HCT)  combined  formulation  was employed  as  a  model.  The  method  (OD\/UV-MCR) involves  on-line  sample  dilution  (OD)  and  UV  detection of  the  analytes,  coupled  to  multivariate  curve  resolution  with  alternating  least  squares  (MCR-ALS). OD\/UV-MCR  proved  to  be  robust  and  was  successfully  validated  in  accordance  to  ICH  guidelines,  fulfilling  acceptance  criteria  for  specificity  (r2 of  spectral  correlation  >  0.950),  linearity  [r  >  0.999  (N  =  25)  in the  ranges  1.00?31.1  mg\/l and  0.51?15.2  mg\/l for  CAR  and  HCT,  respectively]  and precision  (RSD  <  2%). Accuracy  was  assessed  by  point-to-point  comparison between  the  dissolution  profiles  furnished  by  the proposed  method  with  those  provided  by  HPLC  analysis,  evidencing  the  usefulness  of  this  monitoring system.  In  addition,  OD\/UV-MCR  was  successfully  employed  for  the comparative  analysis  of  three  lots  of  commercial  formulations  of  the  CAR?HCT  pharmaceutical  association,  belonging  to  a  couple  of  different brands,  employing  Moore  and  Flanner?s  f2similarity  indicator.","palabras_clave":"MCR-ALS|Green  analytical  chemistry|Simultaneous  dissolution  profiles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1261,"idioma_id":4,"titulo":"Nautiloid cephalopods - a review of their use and potential in biostratigraphy","resumen":"In terms of their use as biostratigraphical tools, nautiloid cephalopods are the poor relations of ammonoids. Nevertheless, in certain situations, they may provide useful biostratigraphical data, particularly where other biostratigraphically valuable taxa are not present; or in certain situations may they demonstrate a resolution as great as, or greater than ammonoids, trilobites, graptolites or conodonts. Nautiloid cephalopods are of especial value in palaeobiogeographical studies, but their use for this purpose may be hampered by the poor understanding of the stratigraphical ranges of individual taxa. The biostratigraphical value of nautiloid cephalopods is demonstrated here through a number of case studies of Ordovician taxa, combined with a review of their biostratigraphical use in Palaeozoic and Mesozoic successions. These both demonstrate the potential of this group and indicate great scope for further research.","palabras_clave":"NAUTILOIDS|CEPHALOPODS|BIOSTRATIGRAPHY|PALAEOZOIC|MESOZOIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1262,"idioma_id":5,"titulo":"De El Pueblo a Esquiú. Modernización  y regresión conservadora frente a la crisis de la prensa católica","resumen":"La investigación histórica sobre la prensa católica constituye un campo de estudios en formación que ofrece análisis relevantes para algunos medios, mientras otros aún esperan por ser abordados. Sin dudas, la revista Criterio fue la más visitada por los historiadores y esto se explica por su extensa trayectoria en la vida cultural del país. A través de sus páginas, y de las plumas reputadas que colaboraron con la revista, fue posible trazar los contornos de una perspectiva católica sobre los avatares de la política argentina, las problemáticas sociales o las cuestiones de género (Rapalo, 1990; De Ruschi Crespo, 1998; Jesús, 2007; Acha, 2000; Rodríguez, 2003). De todas formas, si Criterio puede resultar una puerta de ingreso privilegiada al mundo cultural católico, por esta misma razón es posible que indique poco del conjunto más amplio de católicos que quedaba fuera de los círculos intelectuales.  Si orientamos nuestra mirada hacia la prensa católica de masas -o con pretensiones de serlo- debemos destacar el estudio pormenorizado sobre el diario El Pueblo publicado recientemente por Miranda Lida (2012). El libro constituye el intento más ambicioso por reconstruir la trayectoria del principal diario católico argentino que circuló durante la primera mitad del siglo XX. El análisis de Lida expone diferentes formas de interacción entre el periódico y la sociedad y describe una amplia gama de iniciativas destinadas a consolidar la presencia de la prensa católica. El análisis que ofrece nos resulta sugerente para abordar otro medio católico, la revista Esquiú, por ello en este ensayo tratamos de poner en dialogo sus experiencias. El intento no es arbitrario ya que El Pueblo y Esquiú constituyeron los proyectos periodísticos católicos más importantes del siglo XX. Y si bien cada uno intervino en un contexto social y político muy diferente, hubo un intento de la revista Esquiú por recuperar la trayectoria de El Pueblo y ocupar el lugar que su desaparición, a finales de los 50?, había dejado vacante. No es casual tampoco que los fundadores del semanario Esquiú, los hermanos Luchía Puig, hayan pasado previamente por la dirección de diario fundado por el padre Grote en el 1900.   Nuestra propuesta es retomar el análisis de Lida considerando, particularmente, el momento en que Luis Luchía Puig, desde la dirección de El Pueblo, implementó una serie de estrategias dirigidas a modernizar el diario para relanzarlo como un producto novedoso en la década de 1950. Luego de hacer una breve referencia a esa coyuntura, analizamos más extensamente al semanario Esquiú en la década de 1980 para observar qué respuestas se ensayaron para revertir un momento sumamente crítico para las finanzas del semanario.","palabras_clave":"IGLESIA|PRENSA|EL PUEBLO|ESQUIÚ"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1263,"idioma_id":5,"titulo":"Relaciones estratigráficas de las Formaciones Quebrada de los Colorados y Angastaco (Paleógeno-Neógeno), Valles Calchaquíes, Salta (Argentina): significado en el análisis de la cuenca del Grupo Payogastilla","resumen":"El Grupo Payogastilla representa el registro sedimentario de la cuenca de  antepaís andina en los Valles Calchaquíes. La Formación Quebrada de los Colorados,unidad basal, es de singular importancia debido a que preserva evidencias de los primeros episodios de contracción con deformación intracuencal, cambios en las áreas de aporte y asociaciones de fósiles que la datan paleógena pre-oligocena, hecho que llevó a replantear el modelo de cuenca de antepaís propuesto para el Noroeste argentino.  Asimismo, la suprayacente Formación Angastaco, además de contener similar registro tectono-sedimentario, documenta el inicio del magmatismo en el borde oriental de la Puna. Los recientes trabajos de investigación llevados a cabo en estas unidades han permitido identificar y mapear superficies de discontinuidad sedimentaria, incluso de discordancias que obligan a modificar el cuadro estratigráfico existente. En la presente  contribución se describen las relaciones estratigráficas de la Formación Quebrada de los Colorados con el objetivo de actualizar el conocimiento sobre la misma y discutir el esquema estratigráfico actualmente vigente. Esta formación se asienta en relación de discordancia y de disconformidad sobre las Formaciones Lumbrera, Maíz Gordo y Mealla (Subgrupo Santa Bárbara) y sobre el Subgrupo Pirgua, del Grupo Salta. Asimismo, se  describe y discute el alcance de la identificación de una discontinuidad sedimentaria intra-Quebrada de los Colorados, señalada por el cambio abrupto de facies sedimentarias y la presencia de una superficie de meteorización con desarrollo de una calcreta. Esta superficie separa una sucesión netamente fluvial de otra fluvio-eólica con características  sedimentarias distintivas. Por último, se describen las relaciones de transición y de concordancia entre la sucesión fluvio-eólica con la suprayacente Formación Angastaco. En base a estas relaciones se propone redefinir la Formación Angastaco con la incorporación de la sucesión fluvio-eólica con el rango de Miembro Tin Tin y retomar la definición  original del Miembro Las Flechas. Finalmente y en base la nueva Información disponible se propone a la quebrada Grande (área de cerro Tin Tin) como hipoestratotipo para la Formación Quebrada de los Colorados, localidad que servirá como complemento de las descripciones originales.","palabras_clave":"DISCORDANCIAS|HIPOESTRATOTIPO|EOCENO|ANTEPAÍS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1264,"idioma_id":4,"titulo":"Molecular dynamics simulations of shock-induced plasticity in tantalum","resumen":"We present Non-Equilibrium Molecular Dynamics (NEMD) simulations of shock wave compression along the [001] direction in monocrystalline Tantalum, including pre-existing defects which act as dislocation sources. We use a new Embedded Atom Model (EAM) potential and study the nucleation and evolution of dislocations as a function of shock pressure and loading rise time. We find that the flow stress and dislocation density behind the shock front depend on strain rate. We find excellent agreement with recent experimental results on strength and recovered microstructure, which goes from dislocations to a mixture of dislocations and twins, to twinning dominated response, as the shock pressure increases.","palabras_clave":"Tantalum|Molecular Dynamics|Shocks"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1265,"idioma_id":4,"titulo":"Thermodynamic stability of La2Mo2&#8722;yWyO9, La2Mo2&#8722;yWyO8.96+0.02y and La7Mo7(2&#8722;y)\/2W7y\/2O30 (y = 0, 0.5 and 1.0)","resumen":"The role of W content on the limit oxygen partial pressure (pO2) for stability of fast oxygen-ion conductors  La2Mo2&#8722;yWyO9 with y = 0, 0.5 and 1.0 has been studied by means of thermogravimetric analysis  (TGA) under controlled atmospheres. At 718 °C, below the pO2 stability limit of La2Mo2&#8722;yWyO9, the perovskite  related compounds La7Mo7(2&#8722;y)\/2W7y\/2O30 were stabilized even for y = 1.0. At 608 °C, the first  stage of reduction of &#946;-La2Mo2&#8722;yWyO9 leads to the formation of the crystallized oxygen deficient  La2Mo2&#8722;yWyO8.6+0.02y phase. X-ray powder diffraction shows that the stabilization of the high temperature  &#946;-form through tungsten substitution observed in fully oxidized La2Mo2&#8722;yWyO9 samples is preserved  upon slight reduction. The n-type conductivity arising from the mixed valence state of molybdenum  becomes less and less predominant as the W content increases. Further reduction causes amorphization. At  both temperatures, W substitution does not enhance the thermodynamic stability of the La2Mo2&#8722;yWyO9 ion  conductor under a reducing atmosphere but only slows down the kinetics of reduction.","palabras_clave":"LAMOX|Thermodynamic|stability"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1266,"idioma_id":4,"titulo":"Inheritance and mapping of Mj-2, a new source of root-knot nematode (Meloidogyne javanica) resistance in carrot","resumen":"Root-knot nematodes limit carrot production around the  world by inducing taproot forking and galling deformities  that render carrots unmarketable. In warmer climates,  Meloidogyne javanica and Meloidogyne incognita are most  prevalent. In F2 and F3 progeny from the cross between  an Asian carrot resistant to M. javanica, PI 652188, and a  susceptible carrot, resistance response was incompletely  dominant with a relatively high heritability (H2 = 0.78)  and provided evidence for a single gene, designated Mj-2,  contributing to resistance. Molecular markers linked to the  previously described root-knot nematode resistance gene,  Mj-1 on chromosome 8 derived from ?Brasilia,? demonstrated  that Mj-2 does not map to that same locus but is on  the same chromosome.","palabras_clave":"APIACEAE|DAUCUS CAROTA|MOLECULAR MARKERS|SSRs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1267,"idioma_id":5,"titulo":"Distribución espacial y tasa de invasión de flora exótica en la Reserva Natural de  Vaquerías, Provincia de Córdoba (Argentina)","resumen":"This study was carried out in the Vaquerias Nature Reserve owned by the National University of Cordoba, located in the Department of Punilla, Province of Cordoba-Argentina, during the years 2012-2013. The objective was to obtain information about the process and state of invasion of exotic flora in the reserve and its buffer zone. A baseline survey at landscape scale was undertaken, to make appropriate management decisions for the control of these invasive species. The current spatial configuration (area, shape, and contiguity) of the following species was determined: Ligustrum lucidum (Glossy privet), Gleditsia tricanthos (Honey locust) and Pyracantha coccinea (Scarlet firethorn). The rate of invasion was estimated and priority actions for the management of invasive exotic plants are proposed. The discrimination of plant cover was performed with a SPOT-5 merged satellite image (spatial resolution 2.5 m) using the Parallelepiped classification with an accuracy of 83.84% and an index Kappa of 0.80. Of the 522 ha that form the reserve, invasive alien flora covers a total area of 135.6 ha, distributed as follows: 20.9 ha of Scarlet firethorn, 39.5 ha of Glossy privet and 75.2 ha of Honey locust. In the buffer zone, the quadrants North, South and West represent the areas of greatest risk of invasion to the reserve. The areas dominated by invasion are: roads, trails, streams, creeks, and burnt areas. The invasion rate between 2005 and 2012 for Scarlet firethorn was 0.03 ha\/year and the average increase per year was 49.16%. For the mixed association of Glossy privet and Honey locust the rate of invasion was 0.031 ha\/year and the average increase per year was 4.73%.","palabras_clave":"Ligustrum lucidum|Gleditsia tricanthos|Pyracantha coccinea|tasa de invasión"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1268,"idioma_id":4,"titulo":"Non-enzymatic sensing of carbohydrates using a nickel-chromium alloy electrode.","resumen":"A highly sensitive non-enzymatic carbohydrates sensor based on a nickel hydroxide film on a nickel alloy electrode was developed. The electro-catalytic film is formed by using a potential scan program developedin our research group. This program facilitated the formation of stable proportions of Ni(II)\/Ni(III) oxyhydroxides which are necessary for the electro-catalysis. The structural morphology and roughness of theoxy-hydroxides were characterized by using scanning electron microscopy and atomic force microscopy. The electrochemical behavior of the electrodes in 0.10 M NaOH and in the presence of carbohydrates wascharacterized by cyclic voltammetry and chronoamperometry, showing good catalytic activity for the anodic oxidation of carbohydrates. Fast and significant amperometric responses were obtained with the renewable nickel hydroxide films, which were easily constructed on the electrodes? surfaces by potential cycling. The good analytical performance, low cost and straightforward preparation method make this alloy a promising electrode for the development of effective non-enzymatic carbohydrates sensor.","palabras_clave":"ALEACIONES NIQUEL-CROMO|CARBOHIDRATOS|DETECCION ELECTROQUIMICA|ELECTROCATALISIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1269,"idioma_id":5,"titulo":"Localidades típicas de micromamíferos en Patagonia: el viaje de J. Hatcher en las nacientes del río Chico, Santa Cruz, Argentina","resumen":"ABSTRACT. Type localities of small mammals in Patagonia: The travel of J. Hatcher in the headwaters of the río Chico, Santa Cruz, Argentina. A detailed study of the itinerary followed by J. B. Hatcher and O. A. Peterson in the upper course of the río Chico, Santa Cruz, Argentina, allowed us to discuss the geographic provenance of the holotypes of Ctenomys robustus J. Allen, 1903, Ctenomys sericeus J. Allen, 1903, Oxymycterus microtis J. Allen, 1903, Reithrodon hatcheri J. Allen 1903, Euneomys petersoni J. Allen, 1903, and Notiomys vestitus alleni Osgood, 1925. In this context we discuss and restrict their terrae typicae contributing to the knowledgeof the Patagonian rodent fauna.","palabras_clave":"CHELEMYS|CRICETIDAE|CTENOMYIDAE|CTENOMYS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1270,"idioma_id":5,"titulo":"Construcción multimodal de los argumentos de niños pequeños en disputas durante situaciones de juego","resumen":"En el marco de una perspectiva sociocultural del desarrollo humano y el aprendizaje (Vigotsky, 1964;Bruner, 1986; Nelson, 1996; Tomasello, 1998, 2003), el presente trabajo tiene por objeto indagar la construcción       multimodal de los argumentos producidos por niños de 5 años en el marco de disputas en una situación de juego en  el  jardín  de  infantes.  Para  ello  se  atiende  a  la  yuxtaposición  de  información  que  proporcionan  recursos  de       distintos  campos  semióticos  (Goodwin,  2000,  2007).  El  corpus  está  conformado  por  una  situación  de  juego  de construcción -sala de 5 años- videofilmada, registrada durante el juego en rincones en un jardín de infantes al que       asiste población urbana marginada del Conurbano Bonaerense, Argentina. El análisis sigue una lógica cualitativa a  partir  de  las  herramientas  metodológicas  del  Análisis  de  la  Conversación  utilizadas  en  investigaciones antecedentes (Goodwin, 2000, 2007; Goodwin y Goodwin, 1990, 2000; Goodwin, Goodwin y Yaeger-Dror, 2002). Los  resultados  muestran  que  componentes  de  diversos  campos  semióticos  se  yuxtaponen  con  la  expresión lingüística  de  argumentos  o  puntos  de  vista  que  los  niños  sostienen  en  las  disputas  durante  las  situaciones  de juego. Se  evidencia  la  especial  importancia  de  atender  a  la  entonación,  el  uso  del  espacio,  la  dirección  de  la mirada,  los  gestos  y  el  posicionamiento  corporal  como  componentes  que  conforman  la  fuerza  argumentativa  de       los  enunciados  en  la  disputa.  Asimismo  ellos son  indicios  de  las  emociones  que  las  partes  experimentan  en  las disputas  y  que  no  pueden  ser  obviadas  al momento  de  dar  cuenta  de  cómo  se  produce  la  argumentación  en situaciones reales de interacción.Revista indexada en: Directory of Open  Access Journals, Dialnet y Latindex.  ","palabras_clave":"ARGUMENTACIÓN|NIÑOS|MULTIMODALIDAD|EMOCIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1273,"idioma_id":4,"titulo":"An observer for switched Lipschitz continuous systems","resumen":"In the present work, an alternative approach to the state observation problem for switched Lipschitz continuous systems is proposed. An observer that is based on a concept of observability weaker than that of the instantaneous observability is constructed. Moreover, no strong assumptions are made about the mathematical structure of the observed system. To cope with this limitation, the proposed observer takes advantage of the evaluation of sets of possible estimates. Conditions under which the construction of such sets is possible are found. These conditions assure the state-estimation convergence towards a neighbourhood of the actual system state.","palabras_clave":"NONLINEAR OBSERVER|NON-SMOOTH|LIPSCHITZ CONTINUOUS SYSTEMS|SWITCHED SYSTEMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1274,"idioma_id":4,"titulo":"Generic dynamic model of rechargeable batteries","resumen":"There are numerous models of rechargeable batteries in the current literature. Some of them are complicated electrochemical approaches; others, are given by simple analogies. However, simple models with electrochemical states capable of describing important quantities, like the rate capacity effect  and  the  recovery effect,  are hard to find. In this paper, based on an electrochemical approach, we present a generalized model suitable for use in BMS applications, which takes into account explicitly the  rate capacity effect and the state of charge. Moreover, the model is thought up for general energy storage processes based on mass transport and charge transfer. The proposed  general model approach is able to interpret  the most commonly used models in the literature.","palabras_clave":"BATTERY MODEL| RATE CAPACITY |STATE OF CHARGE |WIENER MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1276,"idioma_id":5,"titulo":"La internacional Socialista en América Latina (1951-1989)","resumen":"            El objetivo de este artículo es presentar los resultados de una investigación sobre las estrategias de la Internacional Socialista (IS) para expandirse en América Latina. El período estudiado abarca, desde su fundación ?en el año 1951-, hasta los sucesos que terminaron con la unificación alemana y la posterior disolución de la Unión Soviética (URSS) en el año 1991.&nbsp;                                               El cuerpo central del artículo se dividirá en cinco partes. Cada una de ellas corresponderá específicamente a las distintas estrategias elaboradas por la IS en una suerte de periodización que busca mostrar la evolución de estas políticas observando sus alcances, cambios y continuidades. La primera estará dedicada a los antecedentes de las relaciones entre los socialistas europeos y América Latina tanto antes de la fundación de la IS como en sus primeros años (1945-1955). La segunda parte se centrará en la primera estrategia formal de la IS para la región: el Secretariado Latinoamericano (1951-1963). En una tercera parte se abordará el cambio de estrategia y la creación de un nuevo organismo interno: el Buró Latinoamericano (1963-1971). En cuarto lugar se analizará la actividad de la organización en un momento de crisis general de la socialdemocracia, observando de qué modo eso repercutió en sus políticas extra europeas (1971-1976). Finalmente, se describirá una nueva etapa organizacional iniciada con la asunción de Willy Brandt en la presidencia de la IS (1976-1992) y caracterizada por ser el momento en que la IS llegó a su momento de máxima expansión e influencia en el mundo en general y en América Latina en particular.&nbsp;                 ","palabras_clave":"INTERNACIONAL SOCIALISTA|SOCIALDEMOCRACIA|AMERICA LATINA|WILLY BRANDT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1277,"idioma_id":5,"titulo":"Estudios de género y renovación de las  Ciencias Sociales en Argentina","resumen":"Se aborda la evolución de la agencia de los derechos de las mujeres y las sexualidades disidentes en la formulación de una agenda académica renovada en las Ciencias Sociales argentinas. Se examina el impacto de la bibliografía crítica  internacional que trajo nuevos retos epistémicos entre los años 1970-1980, y las contribuciones de las académicas feministas latinoamericanaas. La hipótesis que se sostiene es que la investigación científica - que se ha prodigado en la Argentina - tiene alta articulación con la agencia por derechos, y que son esas movilizaciones las nutrientes de la investigación, aunque esta es fundamental para abonar conceptualmente su derrotero.","palabras_clave":"CIENCIAS SOCIALES|GENERO|DERECHOS SEXUALES|ESTUDIOS ACADEMICOS DE GENERO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1279,"idioma_id":4,"titulo":"Development of an Electronic Nose for Determining the Freshness of Fish by the Desorption Constants of Sensors","resumen":"An electronic nose (NE) reduced in size, based on commercial sensors array with a particular thermally stabilized chamber and an advanced processing algorithm was developed. In this work using a heater system that controls the temperature of the sensors chamber, it was possible to study the desorption process on SnO2 sensor array. Data signal of sensors applying a two exponential decay functions were analyzed in order to determine the desorption constants. These constants were used to measure the fish freshness using the PCA algorithm. This is a first approach using this method that shows the ability of the EN to detect interruptions during the cold storage.","palabras_clave":"ELECTRONIC NOSE|FISH FRESHNESS|PATTERN RECOGNITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1280,"idioma_id":4,"titulo":"Effect of indigenous mycobiota on ochratoxin A production by Aspergillus carbonarius isolated from soil","resumen":"This study determined the biotic interaction between30 non-toxigenic indigenous strains of Aspergillus nigeraggregate, Aspergillus flavus, Trichoderma spp., Mucor spp.,Cladosporium spp., Ulocladium spp., Curvularia spp., Absidiaspp., Geotrichum spp. and Acremonium spp., isolated from soildestined for maize crops, with respect to their ability to preventochratoxin A (OTA) production by A. carbonarius on ? in vitro ?assay, on liquid and solid medium. OTA production wascompletely inhibited when A. carbonarius was inoculated in ainteractive mixed culture with all A. niger aggregate strainsassayed, a 80 % of Trichoderma spp. strains, a 40 % ofCladosporium spp. strains, Acremonium spp and Geotrichumspp; only one strain of A. flavus tested was able to completelyinhibit the mycotoxin accumulation. OTA production increasedwhen A. carbonarius ACS 8 was growing on liquid interactivemixed culture with Mucor spp strains. These resultsdemonstrated that OTA production by Aspergillua carbonariusstrain was significantly influenced by the presence of differentnon-toxigenic fungal strains when growing together on paired","palabras_clave":"Aspergillus carbonarius| Indigenous mycobiota|Ochratoxin A|Interacting mixed cultures"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1282,"idioma_id":4,"titulo":"A personal device for analyzing carbon dioxide in real time and real breath: Experimental investigation and computational simulation","resumen":"The analysis of breath CO2 provides valuable information of   pulmonary and cardiovascular functions, and plays a crucial role in   monitoring patients with respiratory problems. Developing portable   sensors for real breath CO2 analysis has been challenging   because exhaled breath is hot, humid and turbulent. In this work, we   have developed, modeled and tested a portable CO2 sensor that can analyze end tidal CO2   concentration in breath and in real time accurately. The key components   of the sensor comprise a fluidic system for efficient breath sample   delivery and a colorimetric detection integrated into the fluidic   system. The modeling includes turbulent mass transport, heat transfer   from the samples at body temperature to the device environment, and   chemical reaction mechanisms, including multiple reactions pathways and   diffusion of CO2 in the sensing layer. Furthermore, the sensor has been tested and compared with a standard commercial CO2 analyzer, and the results are in good agreement with those of the commercial analyzer, and with the modeling.","palabras_clave":"Breath CO2 analysis|3D simulation|Mass transport| Heat transfer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1283,"idioma_id":5,"titulo":"El precondicionamiento isquémico preserva la distrofina a través de la inhibición de la metaloproteinasa de la matriz de tipo 2","resumen":"Introducción: La lesión porisquemia\/reperfusión produce la muerte celular por diferentes vías, algunas delas cuales llevan a la rotura de la membrana plasmática. En los miocitoscardíacos, la distrofina, junto con la espectrina, otorga estabilidad a lamembrana celular, al mismo tiempo que asocia el medio intracelular con elextracelular. La degradación de la distrofina produce fragilidad de lamembrana. Se ha sugerido que el precondicionamiento isquémico es capaz deatenuar este daño; sin embargo, el mecanismo se desconoce. Objetivo: Determinar si el precondicionamiento isquémico previenela degradación de la distrofina a través de la inhibición de lametaloproteinasa de la matriz de tipo 2 (MMP-2). Material y métodos: Corazones aislados de conejo se trataron de lasiguiente manera: G1 (n = 5): perfundidos por 30 min (Nx); G2 (n = 6): 30 minde isquemia global (GI) sin reperfusión; G3 (n = 5): se repitió el protocolo deG2 pero se reperfundió por 180 min (I\/R); G4 (n = 5): los corazones se trataroncon doxiciclina (inhibidor de las MMP) antes de la isquemia global; G5 (n = 6):corazones nor­móxicos tratados con SIN-1 (dador de ONOO-);G6 (n = 5): se administró doxiciclina durante 5 min, previo a la administraciónde SIN-1; G7 y G8 (n = 5): se realizó precondicionamiento previo a 30 min deisquemia con reperfusión y sin reperfusión, respectivamente. La expresión de ladistrofina disminuyó durante la isquemia en un 21% respecto de los valorescontrol (p &lt; 0,05); la expresión de la espectrina se mantuvo sin cambios. Laactividad de la MMP-2 aumentó en un 71% durante la isquemia en comparación conlos valores control (p &lt; 0,05). La administración de doxiciclina antes de laisquemia evitó la degradación de la distrofina. En corazones normóxicos, elSIN-1 aumentó las sustancias reactivas derivadas del ácido tiobarbitúrico(TBARS) en un 33% (p &lt; 0,05) y la actividad de la MMP-2 en un 36% (p &lt;0,05); además, redujo significativamente la expresión de la distrofina a un 23%con respecto a los valores control. El precondicionamiento isquémico atenuó demanera significativa la degradación de la distrofina por inhibición de la actividadde la MMP-2. Conclusiones: La activación de la MMP-2, debido a un aumento en elestrés oxidativo, es responsable de la degradación de la distrofina. Elprecondicionamiento isquémico atenúa la degradación de la distrofina mediantela inhibición de la actividad de la MMP-2.","palabras_clave":"Precondicionamiento isquémico|Distrofina|Metaloproteinasa 2 de la matriz"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1284,"idioma_id":5,"titulo":"LOS DEPÓSITOS EÓLICOS ASOCIADOS A LA PLANICIE ALUVIAL  DEL RÍO DESAGUADERO,PROVINCIA DE MENDOZA, ARGENTINA.","resumen":"Asociado al área de influencia del río Desaguadero, colector principal de los  cursos fluviales que emergen de la región andina central,  se disponen  acumulaciones arenosas eólicas que han recibido escasa atención hasta la fecha. Estudios recientes realizados por los autores del presente trabajo han permitido diferenciar  en ellas una asociación integrada por tres geoformas principales correspondientes a dunas lunetas, dunas longitudinales y mantos arenosos. En conjunto, estos depósitos sobreyacen a la Fm. Arco del Desaguadero, la cual constituye una sucesión fluvio-lacustre desarrollada entre el Pleistoceno tardío y el Holoceno tardío-temprano. Las dunas lunetas están asociadas a la deflación de arenas de playa luego de eventos de enlagunamientos y retracción asociados a crecidas del río Desaguadero que, en función de nuevas dataciones numéricas realizadas sobre restos de conchillas de gasterópodos, permiten referirlos al Optimo Cálido Medieval.  A las dunas lunetas se sobreimponen campos de dunas longitudinales cuyas morfologías bien preservadas, mayormente estabilizadas en la actualidad por la vegetación, se vinculan a procesos de deflación y removilización de arenas  durante la Pequeña Edad de Hielo. En ambos casos, las morfologías desarrolladas indican vientos provenientes del E y SE. Los mantos arenosos se consideran depósitos poligénicos asociados a diferentes ciclos de deflación que afectaron el área.","palabras_clave":"EOLICO|HOLOCENO|DESAGUADERO|MENDOZA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1286,"idioma_id":4,"titulo":"Complementary notes on natural history and reproductive biology of Mecicobothrium thorelli Holmberg 1882 (Araneae: Mecicobothriidae) from Argentina","resumen":"A complementary study of the biology of Mecicobothrium thorelli Holmberg 1882 in Argentina was carried out in the field (Ventania system, Buenos Aires) and laboratory. This species is found under big stones in shady grassland slopes where it builds sheet and funnel webs. Male initiated courtship with body vibrations, palpal drumming and leg tapping when contacted with the female web. Seven copulations were observed with amean duration of 35.73 min, while males performed between 18 and 25 alternate palpal insertions. Maturity molting and sexual activity occur at the beginning of the cool period and this peak of fall\/winter activity could be interpreted as a way to avoid predation.","palabras_clave":"FUNNEL WEBS|ARGENTINEAN MYGALOMORPH|COURTSHIP AND MATING|SPIDER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1287,"idioma_id":4,"titulo":"Atomic entropy squeezing in three-level systems.","resumen":"We consider the problem of an atomic three-level system (in a ladder configuration) interacting with a radiation field. Assuming a coherent state as the initial state, we solve exactly the time evolution of the system. We discuss the appearance of atomic squeezing and calculate the atomic spin squeezing and the atomic entropy squeezing. We show that both parameters predict similar angular and time dependences.","palabras_clave":"ATOMIC SQUEEZING|ATOMIC ENTROPY SQEEZING|THREE-LEVEL ATOMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1289,"idioma_id":4,"titulo":"Elemental profile of patagonian plants after puyehue´s volcanic eruption","resumen":"Entre los años 2011 y 2012 hemos realizado casi 100 muestreos en la Patagonia Norte para analizar el agua, y la composición elemental de algunas especies vegetales fue determinada por SR-µXRF y SR-µXRF. El 4 de junio de 2011 entró en erupción el Complejo Volcánico Puyehue-Cordón Caulle (CVPCC) expulsando unas cien millones de toneladas de cenizas, arena y piedra pómez sobre esta región. De la comparación de los resultados obtenidos en diferentes muestreos, surge que la reciente actividad del CVPCC ha introducido nuevas fuentes de contaminación de origen volcánico en la zona. Los resultados sugieren que la concentración de algunos elementos como Al, Fe, Cu, Zn, Br, Sr está aumentando en algunas especies vegetales expuestas a las cenizas. Debido a que muchas de estas especies son utilizadas como alimentos humanos o del ganado, y que otros elementos minoritarios de la cenizas como el As pueden bioconcentrarse, las técnicas SR-XRF son una valiosa herramienta que está siendo utilizada para analizar periódicamente el perfil elemental de especies vegetales expuestas a las cenizas, a fin de realizar una mejor evaluación de los impactos de este fenómeno.","palabras_clave":"CONTAMINACIÓN VOLCANICA|ARSENICO|PUYEHUE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1291,"idioma_id":4,"titulo":"Karyotype and chromosome variability in the armadillo Chaetophractus villosus, in Argentina.","resumen":"Karyotype and cytotype variations for the large hairy armadillo(Chaetophractus villosus) were studied throughout the species? Argentine distribution. Peripheral blood lymphocyte cultures of 421 animals were used to obtain mitotic metaphases. Preparations were subjected to conventional staining, G- and C-banding, and FISH involving a telomeric probe. Meiotic analysis was performed on testis material  from 10 adults. Spermatocytes were examined for synaptonemal complexes in microspreads. The karyotype (2n = 60XX\/XY; FN = 84 without XY) showed an autosomal complement of 6 metacentric and 7 submetacentric chromosomes;  the remainder was acrocentric. The X chromosome was submetacentric and the Y acrocentric. Centromeric C+ marks were observed in all chromosomes except pair 16. Three NOR signals were detected in 6q, 12p, and 26p. Two chromosomal rearrangements were characterized in chromosome pair 1 a pericentric inversion seen in the material from Jacinto Aráuz, General Madariaga and Pellegrini and a deletion in the material from Loma Verde. Interstitial telomeric signals were observed in chromosome pairs 4, 12, 16, and 26.Pachytene spermatocyte analysis confirmed the basic chromosome  number and morphologies observed in mitotic karyotypes. The evolution of C. villosus involved chromosomal rearrangements as recorded for other species of its msuperorder. The present results establish the basis for the  cytogenetic characterization of this species.","palabras_clave":"ARMADILLOS|CHROMOSOME VARIABILITY|CYTOGENETICS|CYTOTYPES|XENARTHRA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1292,"idioma_id":4,"titulo":"Looking for a face in the crowd: fixation-related potentials in an eye-movement visual search task","resumen":"Despite the compelling contribution of the study of event related potentials (ERPs) and eye movements to cognitive neuroscience, these two approaches have largely evolved independently. We designed an eye-movement visual search paradigm that allowed us to concurrently record EEG and eye movements while subjects were asked to find a hidden target face in a crowded scene with distractor faces. Fixation event-related potentials (fERPs) to target and distractor stimuli showed the emergence of robust sensory components associated with the perception of stimuli and cognitive components associated with the detection of target faces. We compared those components with the ones obtained in a control task at fixation: qualitative similarities as well as differences in terms of scalp topography and latency emerged between the two. By using single trial analyses, fixations to target and distractors could be decoded from the EEG signals above chance level in 11 out of 12 subjects. Our results show that EEG signatures related to cognitive behavior develop across spatially unconstrained exploration of natural scenes and provide a first step towards understanding the mechanisms of target detection during natural search.","palabras_clave":"Visual search|EEG|Faces|Natural scenes|Oddball"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1293,"idioma_id":4,"titulo":"Physicochemical and antimicrobial properties of bovine and salmon gelatin-chitosan films","resumen":"The aim was to study thermo-physical and antimicrobial properties of gelatin-chitosan films by gelatin origin (bovine and salmon), chitosan  concentration and physical state (glassy or rubbery). Thermo-physical properties (pH, Bloom grade, color, isotherms, glass transition  temperature, moisture uptake rate, molecular mobility and film solubility) and antimicrobial effect against Escherichia coli, Salmonella  thyphimurium and Listeria monocitogenes were evaluated. The results showed that the presence of chitosan increased the water content of  both gelatin films and improved the physical performance of both films, especially for salmon gelatin, without a significant (p > 0.05)  change of color. Antimicrobial activity was effective against all bacteria depending on chitosan concentration and glassy or rubbery state,  with the highest effect in glassy state. In conclusion, the evaluated films could be a potential application as bioactive edible films for fresh  foods.","palabras_clave":"BOVINE GELATIN|SALMON GELATIN|CHITOSAN|FILMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1294,"idioma_id":4,"titulo":"Seasonal abundance and vertical distribution of crab larvae from northern Patagonia (Argentina): Implications for their transport in a biogeographic boundary region","resumen":"In species with meroplanktonic larval phases, some reproductive traits (i.e. seasonality and larval behaviour) interact with  hydrographic features to determine larval transport. This work reports the seasonal, diel and ontogenetic changes in vertical  distribution of crab larvae in the boundary region between the Argentine and the Magellanic biogeographic provinces of the  Atlantic coast off northern Patagonia (Golfo Nuevo; 42.758S, 65.008W). Larval seasonality was studied from 47 plankton  hauls taken from August 2004 to July 2005. Vertical distribution was studied from 45 stratified plankton hauls carried out in  August 2005, December 2005 and April 2006. Crab larval stages were detected throughout the year, but abundance was  higher from midwinter to midsummer. Species from southern regions  Munida gregaria, Peltarion spinosulum and  Halicarcinus planatus  were abundant during winter and aggregated mainly near the surface, regardless of the hour of the  day. In contrast, species from northern regions  Platyxanthus patagonicus, Ovalipes trimaculatus, Leucippa pentagona,  Leurocyclus tuberculosus, Cyrtograpsus spp. and Pachycheles chubutensis  were abundant during spring and summer and were  usually aggregated near the surface during the night and dispersed in subsurface layers during the day. Ontogenetic changes  in vertical distribution were not observed. Residual currents reported in the area (3.6 km\/day) and duration of larval life  (approximately 15-70 days) suggests a minimum potential transport on a 100-km scale. Biological information reported in  this work could be explicitly incorporated in a circulation model to accurately predict larval transport and connectivity.","palabras_clave":"DECAPOD|LARVAE|VERTICAL MIGRATIONS|DISPERSAL|SOUTHWESTERN ATLANTIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1296,"idioma_id":5,"titulo":"DETERMINACIÓN POR HPLC Y FTIR DE MANITOL PRESENTE EN LOS EXTRACTOS ACUOSOS DE TRES ESPECIES MEDICINALES DEL CENTRO-OESTE ARGENTINO.","resumen":"Determinación por HPLC y FTIR de manitol presente en los extractos acuosos de tres especies medicinales del centro-oeste argentino.","palabras_clave":"HPLC Y FTIR DE MANITOL |EXTRACTOS ACUOSOS.| TRES ESPECIES MEDICINALES |CENTRO-OESTE ARGENTINO."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1297,"idioma_id":4,"titulo":"Immunization with a chimera consisting of the B subunit of Shiga toxin type 2 and brucella lumazine synthase confers total protection against Shiga toxins in mice","resumen":"The striking feature of Enterohemorrhagic Escherichia coli (EHEC) infections is the39 production of Shiga toxins (Stx) implicated in the development of the life-threatening40 hemolytic-uremic syndrome (HUS). Despite the magnitude of the social impact of41 EHEC infections, no licensed vaccine or effective therapy is presently available for42 human use. One of the biggest challenges is to develop an effective and safe43 immunogen to ensure non toxicity but also a strong input to the immune system to44 induce long-lasting, high affinity antibodies with anti-Stx neutralizing capacity. The45 enzyme lumazine synthase from Brucella spp. (BLS) is a highly stable dimer of46 pentamers and a scaffold with enormous plasticity for the display of foreign antigens on47 it. Taking into account BLS advantages and the potential capacity of the B subunit of48 Stx2 (Stx2B) to induce antibodies that prevent Stx2 toxicity by blocking its entrance to49 the host cells, we engineered a new immunogen inserting Stx2B at the amino termini of50 BLS. The resulting chimera demonstrated a strong capacity to induce long-lasting51 humoral immune response in mice. The chimera induced antibodies with high52 neutralizing capacity for Stx2 and its variants. Moreover, immunized mice were53 completely protected against i.v. Stx2-challenge and weaned mice receiving an oral54 challenge with EHEC were completely protected by the transference of immune sera.55 We conclude that this novel immunogen represents a promising candidate for vaccine or56 antibody development with preventive or therapeutic ends, for use in HUS endemic57 areas or during future outbreaks caused by pathogenic strains of Stx-producing E. coli.","palabras_clave":"Hemolytic Uremic Syndrome|SHIGA TOXIN 2|ANTI-STX2 ANTIBODIES|VACCINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1298,"idioma_id":5,"titulo":"Contingencia  y Derecho: aportes para una radicalización de la crítica jurídica","resumen":"El presente propone a partir de la recuperación de la sociología de Bourdieu, el posestructuralismo de Laclau y la historia de los lenguajes políticos de Rosanvallon, diferentes modos, anudados entre sí, de radicalizar a la crítica jurídica en su distanciamiento de cualquier tratamiento sistémico de lo social y el derecho.  La tesis de este trabajo es que una actitud radicalmente  crítica respecto de lo jurídico involucra dos cuestiones centrales. Por un lado, el cuestionamiento permanente de aquellos fundamentos del derecho aceptados por la doxa jurídica. Lo cual no implica afirmar la inexistencia de fundamentos del derecho sino su carácter inconsistente. Inconsistencia que, a su vez, es concebida como insuperable. Por otro, el énfasis en las instancias fundacionales y\/o dislocatorias de las estructuras jurídicas de sentido. Antes que poner el acento en los modos en que se expresa cierta supuesta coherencia del ordenamiento jurídico, o de las maneras en que se produce el sentido hacia adentro de determinado sistema jurídico, nuestra preocupación se dirige hacia aquellos momentos disruptivos que impiden a todo sistema alcanzar su plenitud. En definitiva, lo planteado lleva a ver en el derecho a un espacio no plenamente medible, calculable y decidible, sino como un terreno primordialmente contingente en donde lo político adquiere un lugar central.","palabras_clave":"Teoría crítica del derecho|Bourdieu |Laclau|Historia de las Ideas."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1299,"idioma_id":5,"titulo":"El nacimiento en la biopolítica: la génesis de la vida en Hegel y Deleuze","resumen":"Este artículo indaga el aporte que el concepto de nacimiento puede realizar al debate en torno de la biopolítica, en tanto no se lo considere como una figura de la reproducción mecánica, sino como una fisura en la trama de la causalidad empírica. Para desarrollar esta perspectiva, se confrontará el rol del nacimiento en los conceptos de vida en Deleuze y Hegel. El concepto de nacimiento así configurado se aplicará a las problemáticas de la biopolítica de manera en que ésta, en lugar de ser meramente un conjunto de tecnologías o mecanismos para la conservación de las relaciones de producción específicas del capitalismo, revele su faz positiva, y sea capaz de introducir nuevas relaciones individuales y sociales.","palabras_clave":"Nacimiento|Biopolítica|Deleuze|Hegel"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1300,"idioma_id":4,"titulo":"Short spatial and temporal scale patterns of fish assemblages in a subtropical rainforest mountain stream","resumen":"The fish fauna of a subtropical mountain stream in Argentina was surveyed for the first time. The abundance and  occurrence of species were explored considering variation in hydrology, seasonality and the abiotic environment.  We collected 221 individuals of 23 species from 10 families and four orders. Small sized species and small  individuals dominated the fish fauna. The small characid Astyanax saguazu was the most conspicuous species.  15 Hydrology, seasonal changes in water temperature and variation in water conductivity affected the abundance and  distribution of fish. Hypobrycon maromba is recorded in Argentina for the first time.","palabras_clave":"Argentina|Misiones|Ramos stream|fish|hydrology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1301,"idioma_id":4,"titulo":"Spartina argentinensis as feedstock for bioethanol and pellets production","resumen":"This paper describes the possibility of producing bioethanol and\/or electricity using pellets from natural rangelands in the Bajos Submeridionales (Submeridional Lowlands) of the province of Santa Fe, Argentina, where the dominant species is Spartina argentinensis, a C4perennial grass with high photosynthetic rate, which tolerates the conditions of alkalinity and the recurrent droughts and floods that shape the region. According to our estimates, enough ethanol could be produced in this region to satisfy the energy demand of vehicles with Otto combustion cycle in several Argentine provinces. Pellets production would allow electricity generation via syngas and power turbines. This would allow the production of liquid fuel and\/or electricity with sustainability criteria, reducing the emission of GHG and generatingjobs, economical growth and development in a depopulated region.","palabras_clave":"Biomass|biofuels|lignocellulose|greenhouse gases (GHG) emissions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1303,"idioma_id":4,"titulo":"Transference of wheat EST-SSRs to Paspalum spp. and cross-species amplification of Paspalum notatum-SSRs: potential use in phylogenetic analysis and mapping","resumen":"Abstract  The genus Paspalum includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat EST-SSR (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum genomic-SSRs (PnSSR) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty two accessions, including 11 species were used. Moreover, 65 F1 hybrids of P. notatum were employed for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer  pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSRs data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both, wEST-SSRs and PnSSRs mapped scattered in the genome. On hundred and two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219 and PN03-F2 generated markers that mapped linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23% to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.","palabras_clave":"Paspalum spp|wheat EST-SSR|Apospory|Comparative mapping"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1305,"idioma_id":5,"titulo":"La perspectiva de género en la formación de jueces y juezas","resumen":"En esta investigación partimos del supuesto de que existe una relación de pertinencia entre la capacitación que reciben quienes aspiran a ocupar o desempeñan cargos en la magistratura y un perfil de juez que colabore con la construcción de un modelo de sociedad igualitario. Por esto nos proponemos analizar la incorporación de la perspectiva de género en la formación de aspirantes a jueces\/zas y jueces\/zas en ejercicio y, en caso de que corresponda, identificar cómo se lleva a cabo la misma y cuáles son las ventajas y desventajas de esa modalidad. De esta manera, en primer lugar, analizamos cómo se vincula la perspectiva de género con las distintas concepciones de igualdad para luego analizar cómo esta perspectiva es incorporada en cuatro casos de oferta de capacitación a nivel federal y provincial o local.","palabras_clave":"Formación judicial |Perspectiva de Género |Escuelas judiciales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1306,"idioma_id":5,"titulo":"Otomicosis por Aspergillus niger","resumen":"El 15% al 20% de las otitis externas son atribuidas a una etiología fúngica, aunque en los últimos años esto parece ir en aumento debido a la utilización de antibióticos de amplio espectro, alteraciones de la inmunidad y uso de corticosteroides, entre otros factores1,2. La otomicosis se define como una infección aguda, subaguda o crónica que afecta al epitelio escamoso del conducto auditivo externo, causada por hongos levaduriformeso filamentosos. La frecuencia y proporción de los agentes etiológicos varía según el área geográfica, pero en general predominan Candidaalbicans y Aspergillus niger3. La colonización fúngica del conducto auditivo no es permanente y puede sufrir variaciones dependiendo de factores ambientales y hábitos higiénicos. Ante determinadas circunstancias estos hongos pueden comportarse como patógenos secundarios, especialmente cuando la piel sufre alteraciones por lesiones mecánicas, acumulación excesiva de cerumen, infecciones bacterianas previas o enfermedades dermatológicas, dando lugar a un proceso infeccioso conocido como otitis externa fúngica u otomicosis1,2,3. El diagnóstico microbiológico se establece con el examen microscópico del material ótico y el cultivo. La patogenicidad se asocia con una concentración fúngica alta en el conducto auditivo y consecuentemente con el examen directo y el cultivo positivos.La colonización, en cambio, se correlaciona con una baja concentración de elementos fúngicos, siendo entonces el examen directo negativo y el cultivo positivo2. En los exámenes directos del materialótico de las infecciones por Aspergillus niger se pueden observar hifas hialinas tabicadas pero, dada la anatomía del conducto auditivo externoy el acceso  al oxígeno que tiene el hongo en esta cavidad, en algunos casos, también es posible observar estructuras de reproducción asexual del hongo como ser conidióforos con sus correspondientes conidios. En las Figuras 1,2 y 3 se observanhifas hialinas tabicadasy estructuras de fructificación asexual de Aspergillus nigeren el material obtenido en una otomicosis y coloreado con tinción de Hematoxilina-Eosina.","palabras_clave":"conducto auditivo|micosis|aspergillus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1307,"idioma_id":5,"titulo":"POTENCIALIDAD HÍDRICA DE LA REGIÓN SANTAFESINA:  EL ACUÍFERO GUARANÍ EN EL TERRITORIO PROVINCIAL.","resumen":"En este trabajo se analiza la problemática del acceso al agua en localidades de la provincia de Santa Fe, revisando el historial del servicio de agua potable en la provincia en los últimos 30 años. El mismo está enmarcado por una carencia de políticas adecuadas donde se aborde de manera integral las problemáticas asociadas con el agua ( control de crecidas, saneamiento y provisión de agua potable). Se analiza la oferta hídrica tanto en lo referente a recursos superficiales como subterráneos, poniendo énfasis en las últimas investigaciones sobre la presencia del acuífero Guaraní en el territorio provincial. A excepción de las localidades que se abastecen del Río Paraná o algunos de sus brazos, el resto de las localidades se abastecen de aguas subterráneas (el 96% del total), y en general, el recurso no es apropiado para consumo humano y requiere costosos de tratamientos de potabilización. La excelente calidad del agua y la osibilidad de captación en el lugar, hacen que el aprovechamiento potencial del Acuífero Guaraní asuma características inigualables para el abastecimiento en nuestra región. Por otra parte, considerando la demanda actual de agua para consumo, no sólo en la provincia sino también en la región se deberá reflexionar sobre los usos para balneoterapia que se le está dando actualmente, sobreexplotando el acuífero y contaminando cuerpos receptores. Resulta imperioso que la legislación respecto a los usos y la protección del Acuífero Guaraní, sea elaborada de manera consensuada por los estados soberanos que comparten el recurso sin intervención alguna de países que son ajenos a los intereses de la región.","palabras_clave":"AGUA POTABLE|PROVINCIA DE SANTA FE|ACUIFERO GUARANI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1308,"idioma_id":5,"titulo":"Valoración nutricional como complemento de los estudios en población expuesta a agentes oxidantes","resumen":"Introducción: el estrés oxidativo (EO) se relaciona con un desequilibrio entre los sistemas oxidativos y antioxidantes, a favor de los primeros. El aporte de micronutrientes de origen dietario podría proveer de un efecto protector ante la exposición laboral a agentes oxidantes.  El objetivo de este trabajo fue determinar si existe relación entre estado nutricional, hábitos higiénico-dietéticos, consumo de macro y micronutrientes y el EO en una población expuesta a agentes oxidantes, en comparación con una población no expuesta.  Material y métodos: se estudiaron dos poblaciones de similares características, a excepción de la actividad laboral de una de ellas, constituidas por personas de ambos sexos, de 18 a 30 años de edad. El Grupo Expuesto estuvo constituido por 26 individuos que trabajaban en servicios de fotocopiado. El Grupo no Expuesto por 27 voluntarios que no realizan dicha actividad laboral. Se realizó una encuesta nutricional, dos recordatorios de 24 h, mediciones antropométricas  y bioquímicas y determinación de marcadores de EO (Catalasa, relación GSH\/  GSSG y TBARS).  Resultados: los resultados se analizaron mediante estadística multivariada para establecer asociaciones entre variables. Se halló una correlación positiva entre actividad laboral y desbalance oxidativo y entre elevado consumo energético, bajo consumo de alimentos fuente de micronutrientes antioxidantes, sedentarismo, alteración del perfil lipídico y EO; y se encontró una relación positiva entre bajo consumo de energía, perfil lipídico normal y adecuado consumo  de frutas y verduras con un estado oxidativo normal.  Conclusiones: los resultados muestran que cambios en los hábitos higiénico-dietéticos podrían ofrecer una protección a sujetos expuestos a agentes oxidantes.","palabras_clave":"EVALUACIÓN NUTRICIONAL|ENCUESTAS NUTRICIONALES|ENCUESTAS DIETÉTICAS|EXPOSICIÓN LABORAL|ESTRÉS OXIDATIVO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1309,"idioma_id":4,"titulo":"Evidence and Control of Bifurcations in a Respiratory System","resumen":"We studied the pressure patterns used by domestic canaries in the production of birdsong. Acoustically different sound elements (?syllables?) were generated by qualitatively different pressure gestures. We found that some ubiquitous transitions between syllables can be interpreted as bifurcations   of a low dimensional dynamical system. We interpreted these results as   evidence supporting a model in which different timescales interact   nonlinearly.","palabras_clave":"bifurcaciones|respiración|canto de aves|control de temperatura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1311,"idioma_id":4,"titulo":"On the existence of extremals for the critical Sobolev immersion with variable exponents","resumen":"In this work we review some recent results conserning the existence problemof an extremal for the immersion W^{1,p(x)}_0 into L^{q(x)} in the critical range.","palabras_clave":"Partial Differential Equations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1312,"idioma_id":5,"titulo":"Culicidae (Diptera) en un area rural del Chaco seco, Argentina","resumen":"To  study  some  ecological  aspects  of  mosquitoes  in  the  Dry  Chaco  phytogeographic region, a study was conducted between February and December 2007 in Misión Nueva Pompeya, Province of Chaco. Three sampled sites: 'Fortin Arenales', 'Los Pozos' and 'Nueva Población' were selected. In each place one CDC-light trap was located in three different sampling stations: extradomiciliary (corresponding to a distant forest between 90 and 110 meters of the home), peridomiciliary   (represented   by   the   corral   up   to   10   meters   distant   from   home)   and intradomiciliary (represented by residents' homes). Twenty one mosquito species corresponding to 8 genera were determined.  The Index of Species Abundance (ISA) and Species Diversity of each sites and sampling stations were estimated. A cluster analysis to group sites and capture stations in terms of abundance and diversity of species was performed.  Mansonia titillans (Walker) was the most abundant and common species in the study area, followed by Uranotaenia   apicalis  (Theobald)  and  Anopheles  triannulatus  (Neiva  and  Pinto).  Regarding  seasonality, monthly captures revealed a winter station with little or no presence of mosquitoes  and a rainy season (summer- autumn station) with higher abundance, species richness and diversity.","palabras_clave":"CULICIDAE|DISTRIBUCIÓN|ARGENTINA|MOSQUITOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1313,"idioma_id":5,"titulo":"COMBATES POR LA EPOPEYA GAUCHA LA FORMACIÓN DEL CAMPO CULTURAL SALTEÑO: BERNARDO FRÍAS, JUAN CARLOS DÁVALOS Y JOAQUÍN CASTELLANOS, 1870-1931.","resumen":"La siguiente comunicación  propone estudiar la formación del campo intelectual en Salta a través del  análisis de la producción historiográfica de Bernardo Frías y la literatura de  Juan Carlos Dávalos como obras centrales de la construcción identitaria local.  Cada uno contribuyó de manera específica a la reivindicación de Martín Miguel  de Güemes como emblema cultural salteño a través del cual cuestionar las  interpretaciones del pasado elaboradas en Buenos Aires. En el nuevo clima de ideas inaugurado en torno al Centenario las  representaciones de Frías y Dávalos  habilitaron nuevos sentidos. La revalorización del gaucho y su integración a  una cultura criolla en formación permitieron que Güemes se convirtiera en un  héroe nacional. La consolidación del campo cultural local se produjo en un  contexto político conflictivo, marcado por el enfrentamiento del gobierno  reformista de Joaquín Castellanos con un sector conservador de la elite salteña. El empleo del mito güemesiano en el conflicto político local actualizó una nueva lucha por la atribución de  sentido a la figura de Güemes coherente con estos proyectos políticos rivales.","palabras_clave":"IDENTIDAD SALTEÑA|GÜEMES|CONFLICTO POLÍTICO|CAMPO CULTURAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1314,"idioma_id":4,"titulo":"Resistance sources to stem and pod blight of soybean caused by two Phomopsis especies in Argentina","resumen":"RESISTANCE SOURCES TO STEM AND POD BLIGHT OF SOYBEAN CAUSED BY TWO Phomopsis ESPECIES IN ARGENTINA. HernándezF.;Faraco L.; Malik C.; Pratta G.,Pioli R.  Plant Pathology;Plant and Microbial Biodiversity Area;Genetic;Faculty of Agricultural Sciences.U.N.Rosario. Zavalla. E-mail:rpioli@unr.edu.ar    Stem and pod blight is a disease associated to the reproductive stages of soybean (Glycine max), that depress the sanitary and physiological quality of seeds.The aim of this study was to assess the behaviour of four soybean resistance sources to interact with P. longicolla (Plo) and P. phaseoli var. sojae (Pps) (teleomorph: Diaporthephaseolorum var. sojae, Dps), both, the causal agents of the disease. In greenhouse conditions, experimental design included as plant germplasm: four soybean genotypes carrying one or two Rpsd genes and two genotypes of reaction non known, and as fungalgermplasm: seven Plo isolates, one Dps, and two experimental controls (one each D. phaseolorum var. meridionalisand var. caulivora). Inoculations were made with a micro needle to produce a micro-injury and introduce 5µl of a conidial (co) suspension (1.105-6 co \/ ml) in the axil of the first and third trifoliate leaves.Results were analyzed by a factorial with five categories (6 genotypes, 10 fungi, 3 pots, 4 plants, 5 internodes) and LSD Fischer Test. Dependent variable analyzed was severity (% infected area) of internodes \/ plant. As result of all interactions,genotypes carrying two Rpsd genes (Rpsd1 y Rpsd2) y (Rpsd2 y Rpsd3) were the most resistant, while, Plo7 (from Tucumán) and Plo1 (from Santa Fe) were the isolates most virulent.Specific interactions related toplant susceptibility \/ pathogen virulence as plantResistance \/ pathogen Avirulencewere also detected. The results showed an advance in the knowledge of this interaction and its possible application in plant breeding.","palabras_clave":"SOYBEAN|REPRODUCTIVE STAGE DISEASES|STEM BLIGHT AND SEED DECAY|RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1315,"idioma_id":5,"titulo":"RESPUESTA DE UNA POBLACIÓN DE MALEZAS DE Lolium perenne L. AL USO CONTINUO DE DOSIS SUB-RECOMENDADAS DE GLIFOSATO","resumen":"El rye grass (Lolium spp.) es&nbsp; una de las principales malezas de  cereales de invierno del sur bonaerense, su manejo se ha basado en el uso de  glifosato, a menudo a dosis inferiores a las recomendadas. Desde el 2006, se  advierte en esa región la sobrevivencia a glifosato de Lolium perenne L., luego  de una historia de más de 10 años de utilización del herbicida. Se evaluó el  comportamiento de plantas problema ante aplicaciones de diferentes dosis de  glifosato. &nbsp;Contrastando con plantas susceptibles, las plantas problema  tratadas con glifosato resultaron insensibles a dosis normalmente letales. Se  determinó que para igualar la eficiencia de control en plantas resistentes  respecto a susceptibles se requiere incrementar la dosis 10,8 veces. Se  cuantificó la acumulación de shikimato en hojas a las 72h post-aplicación, este  metabolito es indicador de la susceptibilidad al glifosato, detectando  diferencias significativas entre ambas poblaciones. Se concluye que en la  población problema existen plantas resistentes a glifosato estás se habrían  incrementado su frecuencia resultado de una continua presión de selección con  el herbicida. Este antecedente indica que es imprescindible garantizar la  rotación de principios activos para el empleo del control químico como parte  del manejo integrado de malezas.","palabras_clave":"resistencia|malezas|herbicida|glifosato"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1320,"idioma_id":5,"titulo":"Compilación de Observatorios","resumen":"COMPILACIÓN DE INFORMES DE OBSERVATORIOS CITRADIS","palabras_clave":"PROTECCIÓN SOCIAL|SALARIO MÍNIMO|SEGREGACIÓN RESIDENCIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1321,"idioma_id":5,"titulo":"ANTUMPA, UN PAISAJE PRODUCTIVO DEL PRIMER MILENIO A.D. EN EL SECTOR NORTE DE LA QUEBRADA DE HUMAHUACA","resumen":"Antumpa es un sitio arqueológico ubicado en el sector norte de la Quebrada de Humahuaca(Prov. de Jujuy) que posee un importante componente correspondiente al primer milenio A.D. Conocido tradicionalmente por ser uno de los pocos sitios para ese rango temporal en el ámbito de la Quebrada de Humahuaca, nuevas investigaciones han comenzado a aportar  información relevante acerca de su ocupación, trazado espacial y cronología. En este trabajo,abordamos a Antumpa como un paisaje productivo, entendiéndolo como una compleja intervención humana en el entorno en función del desarrollo de variadas actividades económicas  y sociales. De esta manera, se trascienden los límites físicos que lo han definido tradicionalmente como ?sitio?, para incluir el entorno adyacente, donde se ubican diversas evidencias de menor visibilidad arqueológica pero de gran importancia para comprender cómo se construía  y significaba el espacio a través de actividades cotidianas y rituales. Antumpa se presenta entonces como una compleja trama de interacciones entre personas, prácticas, rasgos geográficos naturales, artefactos y estructuras diversas, extendida sobre una amplia superficie y a través de varios siglos. El trabajo intenta dar cuenta de esta complejidad, presentando información procedente de distintas líneas de investigación e integrándola hipotéticamente bajo la noción de paisaje productivo.","palabras_clave":"Antumpa|Quebrada de Humahuaca|Paisaje productivo|Primer milenio A.D."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1322,"idioma_id":5,"titulo":"El uso de sustancias psicoactivas: la situación en institutos de educación secundaria de Córdoba","resumen":"A partir de la revisión bibliográfica realizada, se evidenció la necesidad de estudiar la combinación de los aspectos escolares y el consumo de sustancias en la institución escolar.   Objetivos: Describir y analizar consumo de sustancias psicoactivas, condiciones de las instituciones educativas y su relación con la situación escolar de los estudiantes.  Metodología: En una primera etapa se abordó la perspectiva cuantitativa utilizando la base de los registros de la Segunda Encuesta Nacional a Estudiantes de Enseñanza Media (2005) de Córdoba y en una segunda se abordó la perspectiva cualitativa. Desde la cuantitativa se trabajó con una muestra probabilística estratificada polietápica (n=4593). El análisis se efectuó a través de medidas de resumen, análisis multivariado y factorial de correspondencia. En todos los casos se estableció un nivel de significación de p< 0,05. En la etapa cualitativa se realizó un estudio etnográfico. La selección de escuelas de gestión pública (oficiales y privadas) se efectuó por muestreo intencionado, acumulativo y secuencial. Se realizaron 10 entrevistas en profundidad. El análisis de la información cualitativa se ejecutó a través del método comparativo constante.   Resultados y conclusión: Se evidenciaron diferencias de consumo de drogas legales e ilegales entre los estudiantes según jornada de estudio (p<0,001). La repitencia escolar y los problemas de comportamientos se asociaron con el consumo de alguna droga ilícita. La escuela organizada en función a su finalidad educativa, la exigencia académica y la claridad en las normas se constituyeron en instituciones en donde asisten adolescentes que  no consumen sustancias psicoactivas.","palabras_clave":"EDUCACIÓN|ADOLESCENTES|TRASTORNOS RELACIONADOS CON SUSTANCIAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1323,"idioma_id":5,"titulo":"Mejora en la recuperación de Au y Ag mediante cianuración en escorias de fundición","resumen":"El objetivo del trabajo fue recuperar y mejorar la obtención de oro y plata, presentes en escorias de fundición, utilizando los mismos procesos metalúrgicos que en menas de oro. La escoria se concentró en una centrífuga tipo Knelson, obteniendo un concentrado (C1) y una cola (T1). Para mejorar la recuperación, se realizó la lixiviación intensiva de T1. Las variables utilizadas fueron: tamaño de partícula, concentración de NaCN (2000 y 4000 g\/cm3) y aireación, siendo el tiempo de residencia 120 minutos. Se concluye que la recuperación de Au, aplicando concentración por gravedad (centrífuga Knelson) es del 83.6 %, siendo la recuperación de Ag del 52.5 %. La recuperación de Au se mejora en un 90.4 % a través de la lixiviación de la cola de la separación centrífuga y 45.6 % de Ag. Siendo las condiciones óptimas: 4000 g\/cm3 de NaCN y aireación artificial, tamaño [-14 # ? +35#], en un tiempo de 98 horas.","palabras_clave":"lixiviación intensiva;|concentración gravitacinal|proceso metalúrgicos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1324,"idioma_id":5,"titulo":"Salpichroa origanifolia y Solanum sisymbriifolium (Solanaceae): Micrografia Analitica Comparativa de Hojas y Frutos","resumen":"Salpichroa origanifolia (Lam) Baill., huevo de gallo o uva de campo y  Solanum sisymbriifolium Lam., revienta caballo, tomatillo o tutiá son malezas  de la familia Solanaceae cuyos frutos suelen ser comestibles y sus hojas  medicinales. Mediante el método micrográfico (técnicas de disociado leve y raspado)  se caracterizan ambos órganos. Los caracteres de valor diagnóstico los  aportan los pelos: foliares, del cáliz y de la testa seminal.  Salpichroa origanifolia presenta pelos tectores bi a pentacelulares y glandulares  con pie tri a pentacelular y cabeza secretora unicelular redondeada u  ovoide o pie unicelular y cabeza secretora unicelular redondeada; en el fruto la  cutícula es estriada y si hay restos de cáliz están presentes pelos glandulares  cortos y largos, de pie bi a tetracelular y cabeza secretora uni a bicelular; las  semillas son pilosas.  En Solanum sisymbriifolium los pelos tectores son uni a bicelulares o con  célula apical estrellada; los pelos glandulares poseen pie uni a tetracelular y  cabeza secretora bicelular o pie unicelular corto y cabeza secretora unicelular;  en el fruto se detectan esclereidas; las semillas poseen esclereidas de bordes  ondulados.  Estos caracteres pueden ser usados para identificar hojas y frutos de  ambas especies cuando integran un producto en cuya elaboración el material  ha sido triturado.","palabras_clave":"Salpichroa origanifolia|Solanum sisymbriifolium|malezas alimenticias|plantas medicinales|micrografía"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1325,"idioma_id":5,"titulo":"'ESCRIBIR LAS PRÁCTICAS'. UNA ACERCAMIENTO A CIERTAS PRODUCCIONES ESCRITAS DE LAS ORGANIZACIONES ARMADAS PERONISTAS EN LOS PRIMEROS AÑOS SETENTAS","resumen":"Revista INDIZADA en: Latindex,DialNet  Desde mediados de los 90´s gran parte de la historiografía se construyó sobre la insistencia por encontrar elementos comunes que permitiesen caracterizar la 'militancia setentista'. Los debates en torno al binomio política y\/o violencia han nutrido gran parte de los estudios sobre ese pasado. No obstante, de ese conjunto de investigaciones que han aportado significativamente al desarrollo de nuevas miradas sobre un viejo problema, pocos se han interrogado sobre las teorías que sostuvieron dichas elecciones. En este artículo nos proponemos indagar en aquellas formulaciones de carácter teórico que acompañaron el devenir de una organización política de los primeros años setenta: Montoneros Sabino Navarro en un diálogo implícito con los Montoneros.","palabras_clave":"Sabino Navarro|Montoneros|alternativismo|clasismo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1327,"idioma_id":5,"titulo":"Escritura en la formación docente: los géneros de la práctica","resumen":"    La  relación entre práctica docente y escritura se constituye en un tipo de  relación productiva hasta ahora escasamente indagada y que viene demostrando  cierta productividad por su impacto directo en los procesos de enseñanza y  formación. El propósito de este artículo es dar a conocer algunas líneas de  trabajo de investigación relacionadas con experiencias de formación docente, en  el área de letras, que dan relevancia a la práctica de escritura como  configuradora de los procesos de construcción y de reflexión de la práctica de  enseñanza.   ","palabras_clave":"ENSEÑANZA|FORMACIÓN |ESCRITURA|PRÁCTICAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1328,"idioma_id":4,"titulo":"Accelerator technology and SPECT developments for BNCT","resumen":"Accelerator-Based Boron Neutron Capture Therapy (AB-BNCT) is establishing itself worldwide as the future modality to start the phase of in-hospital facilities. There are projects in Russia, UK, Italy, Japan, Israel and Argentina to develop AB-BNCT around different types of accelerators.In particular, the present status and recent progress of the Argentine project for the development of a Tandem-Electrostatic-Quadrupole (TESQ) accelerator is presented. Different working areas are treated: high-power ion sources, acceleration tubes, transport of intense beams, beam diagnostics, high-power targets, 9Be(d,n) reaction as a possible neutron source and treatment room design. A complete test stand was built and commissioned for intense protonbeam production and characterization. Beams of 10 to 30 mA have been produced and transported during variable periods of operation by means of a pre accelerator and an electrostatic quadrupole doublet to a suppressed Faraday cup. The beam diagnostics has been performed through the observation with digital cameras of induced fluorescence in the residual gas. A 200 kV TESQ accelerator prototype has been constructed and is under test and a 600 keV prototypeis under construction. Self consistent space charge beam transport simulations have been performed and compared with experiments. In addition to the traditional 7Li(p,n) reaction, 9Be(d,n) using a thin Be target has been thoroughly studied as a candidate for a possible neutron source for deep seated tumors, showing a satisfactory performance. A treatment room complying withregulations has been designed. Finally we present advances in the development of a Single-Photon Emission Computed Tomography (SPECT) system for online dosimetry during a BNCT treatment.","palabras_clave":"BNCT|SPECT|ACCELERATOR TECHNOLOGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1329,"idioma_id":4,"titulo":"Preparation and characterization of polymorphs of the glucocorticoid deflazacort","resumen":"The pharmaceutical polymorphism of new and old active pharmaceutical ingredients (APIs) is of great importance due to performance, stability and processability aspects. The objective of this study was to investigate the polymorphism of deflazacort (DEF), a glucocorticoid discovered more than 40 years ago, since this phenomenon has not been previously investigated for this API. Using different methods for solid form screening, it was determined for the first time that DEF is able to exist as three forms: a crystalline one (DEF-1); a hydrated X-ray amorphous form (DEF-t-bw) and an anhydrous amorphous phase (DEF-g) obtained from manually grinding DEF-1. The in vitro and in vivo dissolution rates (DRs) of DEF-1 and DEF-t-bw, which were measured using the rotating disk method in water at 37 °C and the pellet implantation technique in rats respectively, indicated  that DEF-t-bw  exhibited   slightly faster in vitro and in vivo DRs than those of DEF-1, but the values were not significantly different. In addition, it was determined that DEF-t-bw easily devitrifies to DEF-1 by the effect of pressure, humidity and heat. It was concluded that DEF is glucorticoid with low tendency to exhibit different crystalline anhydrous and solvated forms and that DEF-t-bw has no advantages over DEF-1 in terms of solubility, DRs and solid-state stability.","palabras_clave":"polymorphism|deflazacorth|amorphous"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1330,"idioma_id":5,"titulo":"Los senderos del sentido conducen al cuerpo: Wittgenstein, el sentido práctico y la encarnación","resumen":"Si bien la llamada segunda filosofía de Wittgenstein ha sido vista como una filosofía preocupada por el lenguaje, esta es primeramente una filosofía acerca de las prácticas humanas como enraizadas en contextos de sentido que Wittgenstein denomina formas de vida. En Sobre la certeza, discute la perspectiva de Moore respecto del asidero de nuestras proferencias de sentido común, discusión que ha sido interpretada de un modo pobre que desconoce los senderos a los que Wittgenstein nos lleva de la mano de su subversiva mirada de la filosofía como pensamiento crítico, sendero que nos lleva desde la mirada de agentes de sentido en tanto agentes prácticos enraizados a agentes encarnados. En el presente trabajo recorreremos el camino wittgensteniano que comienza con el intento de anclar el hombre común, el sentido de sus prácticas en sus formas de vida y permite vislumbrar cuerpo\/encarnación\/prácticas como nodos de sentido que permiten revertir\/cuestionar\/subvertir la actitud naturalizada, y cuestionable, del olvido del cuerpo en el ámbito de las ciencias sociales.","palabras_clave":"Cuerpo|Sentido Práctico|Wittgenstein|Hábitus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1331,"idioma_id":5,"titulo":"Dieta de la población de guanacos (Lama guanicoe) reintroducida en el Parque Nacional Quebrada del Condorito, Argentina","resumen":"La reintroducción de especies silvestres es una herramienta recomendada cuando favorece la funcionalidad del ecosistema y se realiza en un proceso a largo plazo. Por ello es necesario evaluar científicamente los distintos aspectos ecológicos involucrados en el proceso de reintroducción. En el caso de los grandes herbívoros silvestres un aspecto importante es conocer los hábitos de forrajeo. En el presente estudio se determinó la dieta a nivel de especie de la población de guanacos (Lama guanicoe) reintroducida en el Parque Nacional Quebrada del Condorito (Córdoba, Argentina). Para ello entre febrero y agosto de 2009 se recolectaron muestras de heces frescas en del territorio ocupado por los grupos reproductivos dentro del Parque. La composición botánica de las muestras se determinó mediante análisis microhistológico. La dieta de los guanacos reintroducidos estuvo constituida principalmente por gramíneas y ciperáceas de bajo porte, características de los céspedes, y, a pesar de la alta diversidad de plantas vasculares presentes en el área de estudio, solo cinco especies representaron entre el 71 % (para los meses fríos y secos) y el 93 % (para los meses cálidos y húmedos) de la dieta consumida durante el período de estudio: Sorghastrum pellitum, Chascolytrum subaristatum, Carex fuscula, Eleocharis pseudoalbibracteata y Lachemilla pinnata. Asimismo, las cuatro primeras especies fueron consumidas en una proporción significativamente mayor a la abundancia en el campo, lo que estaría indicando que los guanacos reintroducidos se comportaron como consumidores selectivos. Por otra parte se detectó una variación estacional pequeña pero consistente en el consumo de las especies vegetales, incrementándose en los meses fríos y secos el consumo de  las gramíneas de alto porte D. hieronymi y P. stuckertii, dominantes en los pajonales, y de especies leñosas. Esto se debe probablemente a la disminución de la productividad durante los meses fríos y secos en el área de estudio.","palabras_clave":"análisis microhistológico|herbívoría|Sierras Grandes del Centro de Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1332,"idioma_id":5,"titulo":"SUPERVIVENCIA DE GUANACOS (Lama guanicoe) REINTRODUCIDOS CON Y SIN PERÍODO DE PRE-ADAPATACIÓN EN EL PARQUE NACIONAL QUEBRADA DEL CONDORITO, ARGENTINA","resumen":"El Parque Nacional Quebrada del Condorito se ubica en la porción superior de las Sierras Grandes del Centro de Argentina. Desde su creación el ganado doméstico fue erradicado con el fin de evitar los procesos erosivos producto del sobrepastoreo. Sin embargo, la ausencia de herbívora a gran escala produjo el avance de los Pajonales sobre las demás comunidades vegetales del Parque, disminuyendo así su biodiversidad. Por este motivo la Administración de Parques Nacionales decidió reintroducir el guanaco (Lama guanicoe), extinto en la región durante la conquista española. En el año 2007 se realizó una liberación de 113 individuos sin que éstos pasaran por un período de pre-adaptación, y en 2011\/2012 se liberaron 25 individuos que si pasaron por un período de pre-adaptación. Se comparó la supervivencia entre ambas técnicas de liberación,  cuatro semanas y  año respectivamente después de la liberación. Los resultados mostraron que la supervivencia inicial de los guanacos liberados luego de un período de pre-adaptación fue significativamente superior con relación a los liberados sin haber pasado por el período de pre-adaptación. Los individuos que sobrevivieron el período crítico post-liberación (cuatro semanas) no presentaron diferencias en la supervivencia registrada hasta el año de haber sido liberados. Por lo tanto, se recomienda que las futuras liberaciones de guanacos se realicen luego de que los individuos pasen por un período de pre-adaptación,  hasta que la población  reintroducida alcance un tamaño mínimo viable que permita que la especie cumpla el rol ecológico para el cuál fue reintroducida en el Parque.","palabras_clave":"Córdoba|Lama guanicoe|Reintroducción|Sierras Pampeanas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1333,"idioma_id":5,"titulo":"La buena enseñanza en la Educación Superior. Profesores memorables y Valores morales","resumen":"En este artículo nos proponemos indagar cuáles son los valores y conductas morales que caracterizan la enseñanza de los profesores considerados memorables por estudiantes avanzados de las carreras de profesorado de la Facultad de Humanidades de la Universidad Nacional de Mar del Plata, Argentina, con detenimiento en cuestiones asociadas al compromiso y la responsabilidad. La buena enseñanza involucra aspectos morales que inciden favorablemente en el aprendizaje. Identificamos,a partir de entrevistas biográficas a los profesores memorables, cuáles son los valores, actitudes y conductas morales que caracterizan su enseñanza,con el fin de encontrar las continuidades y rupturas metodológicas que permitan analizar categorías que contribuyan al conocimiento del desarrollo profesional docente y de problemáticas asociadas a la didáctica del nivel superior. La metodología adoptada corresponde a la investigación biográfico-narrativa, en el marco de la investigación cualitativa.","palabras_clave":"PROFESORES MEMORABLES|BUENA ENSEÑANZA|VALORES MORALES|DIDÁCTICA DEL NIVEL SUPERIOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1334,"idioma_id":5,"titulo":"NACAO, MEMORIA E RESPONSABILIDADE. A NACAO FRENTE AOS CRIMES DO ESTADO","resumen":"Con el surgimiento de un nuevo régimen de memoria basado en la figura de la víctima y la noción de responsabilidad, los Estados-Nación se ven la necesidad de desplegar estrategias y políticas tendientes a hacer frente a los crímenes cometidos en su nombre. A partir del análisis de instituciones y políticas de memoria sui generis, este artículo se ocupa de las reconfiguraciones de la relación entre nación, memoria y responsabilidad.","palabras_clave":"NACION|RESPONSABILIDAD|MEMORIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1335,"idioma_id":4,"titulo":"An Argentinean innovation and technology management specialization programme: the GTec case study","resumen":"Using commonly accepted methodology employed for industrialized countries,  a study was made in Argentina?s western region to compare local performance  in innovation vs. UE. There are several papers and works that reflect  the main stream of innovation in Argentina and this material demonstrates  the way of thinking that this country uses to improve its performance. The  fact that innovation is a key element in firms? performance is well established  in developed countries. A comparison based on the European Community  Innovation Surveys (CIS), among other sources, has been made to establish  which practices and lectures have been used to achieve success in UE  and discover whether these ?practices and lectures? are part of the agenda  in innovation programmes or innovator training in the Argentine middle  western region. The aim is to find out more accurately, which factors are  influencing the innovation process and measure the impact that some ideas  and lectures on innovation have on performance; especially for innovator  managers in the SME sector.","palabras_clave":"INNOVACION |TECNOLOGIA |GESTION |ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1336,"idioma_id":5,"titulo":"Comunidades microbianas extremófilas en el sistema geotermal del volcán Copahue y sus aplicaciones biotecnológicas","resumen":"Comunidades microbianas extremófilas en el sistema geotermal del volcán Copahue y sus aplicaciones biotecnológicas","palabras_clave":"EXTREMOFILOS|COPAHUE|GEOTERMAL|BIOTECNOLOGIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1337,"idioma_id":4,"titulo":"Corrosion of painted galvanneal steel","resumen":"The annealing galvanized steel (Galvanneal) is produced from hot-dip galvanized steel thermally treated. The result is a coatingformed by Fe-Zn phases. Its main advantage over the conventional galvanized steel is the absence of the characteristic spanglesaffecting these coatings and the presence of iron providing better weldability than the pure zinc coating. In this work, thecorrosion behavior of pre-treated and painted with environmentally friendly schemes, conventionally hot-dip galvanized, andannealing galvanized (Galvanneal) steel were studied. A &#947;-mercaptopropyltrimethoxysilane (MTMO) pre-treatment was applied.A waterborne polyurethane paint developed at CIDEPINT was used. Assays were performed in the salt spray and controlledhumidity chambers. The metal-paint adhesion was determined by Tape Test. The systems deterioration was evaluated by meansof periodical visual inspections, optic and electron microscopes, EDXS, and electrochemical impedance measurements. TheMTMO showed to be a good adhesion promoter for the systems exposed to the present testing conditions.","palabras_clave":"GALVANNEAL|GALVANIZED STEEL|CORROSION|PAINT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1338,"idioma_id":5,"titulo":"Modelado del potencial bioactivo y calidad de frutillas frescas cortadas en el lavado ? desinfección con ácido peracético","resumen":"          En  este trabajo se propone cuantificar y modelar los cambios en el potencial  saludable, atributos fisicoquímicos y reducción de microorganismos aerobios  mesófilos en dos variedades de frutillas frescas cortadas (Camarosa y Selva)  cuando se someten al lavado-desinfección con soluciones de ácido peracético  (APA), variando la concentración (0 ? 100 mg L-1), el tiempo de  contacto (10 ? 120 s) y la temperatura de la solución de lavado (4 ? 40°C). Para el diseño y  análisis de los resultados se aplicó la Metodología de Superficie de Respuesta, siendo  las respuestas: porcentajes de retención de acido ascórbico (AA) y vitamina C  (Vit C), antocianinas totales (Ant T), fenoles totales (FT), capacidad  antioxidante (CA), y sólidos solubles (SS), cambios de pH, acidez total (AT), y  cambios de los parámetros de color: L*, a*, b*, Cab* y hab;y reducción de la flora aerobia mesófila (FAM). Los modelos de  retenciones de AA, Ant T, CA y FT se vieron afectados por las variables del proceso, principalmente por la  concentración de APA y el tiempo y no se observaron diferencias  entre ambas variedades. La  retención de Vit C y los cambios de color no se vieron afectados por las  variables de procesamiento para el cultivar Camarosa.  Sin embargo, para la variedad Selva, los  modelos predictivos de estos parámetros si se vieron afectados por las  variables de la operación. La  retención de SS, y los cambios de pH y AT no fueron afectados por el lavado, y  por lo tanto no pudieron modelarse. Por otra  parte, la Red FAM  fue afectada por las variables del proceso obteniéndose modelos predictivos  para cada cultivar. Este trabajo demuestra el  comportamiento diferente de ambas variedades de frutillas ante un mismo proceso  de lavado-desinfección y provee herramientas predictivas sencillas para  cuantificar dicho efecto.  ","palabras_clave":"ACIDO ASCORBICO|ANTOCIANINAS TOTALES|COLOR|MICROORGANISMOS AEROBIOS TOTALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1339,"idioma_id":4,"titulo":"Nieblas del Riachuelo. Cuencas hídricas y cambio climático en Buenos Aires. El conflicto por el saneamiento de la cuenca Matanza-Riachuelo como caso testigo.","resumen":"En este trabajo nos proponemos avanzar en una línea de indagación referida a los escenarios de cambio climático en la Región Metropolitana de Buenos Aires de cara a los grandes déficits existentes en materia de gobernabilidad ambiental. Tomaremos como punto de referencia las políticas de gestión hídrica y prestaremos atención a la capacidad institucional efectivamente existente para encarar medidas de adaptación. En este sentido, consideramos que las acciones en materia de adaptación deben poner atención a los grupos sociales más expuestos en materia de condiciones sociales y sanitarias    Nota sobre la revista:  Latin American Perspectives se encuentra indexada en Scopus y en el Social Sciences Citation Index.","palabras_clave":"cambio climático|Política|adaptación"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1340,"idioma_id":4,"titulo":"An Analytical Solution of Tide-Induced Head Fluctuations in an Inhomogeneous Coastal Aquifer","resumen":"A new analytical solution for tide-induced head fluctuations in a confined aquifer with hydraulic conductivity that increases quadratically with the distance to the coastline is presented. To derive the analytical solution the following assumptions are made: the aquifer ends at the coastline and extends landward infinitely, the water flow is horizontal and obeys Darcy?s law, the specific storativity is constant, and the sea tide is described by a sinusoidal function. The proposed analytical solution has a relatively simple mathematical expression and it is used to estimate the hydraulic conductivity from experimental data. In general terms, it can be concluded that the quadratic heterogeneity produces dampened amplitudes near the coastline and a faster transmission of the tidal effect into the aquifer.","palabras_clave":"ANALYTICAL SOLUTION|GROUNDWATER FLOW|TIDAL FLUCTUATIONS|COASTAL AQUIFER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1341,"idioma_id":5,"titulo":"La Tecnología al servicio de la gestión universitaria","resumen":"El presente trabajo describe la aplicación de un Sistema Informático de Consulta de Alumnos (SICA) desarrollado con el fin de dar respuesta a un número de dificultades administrativas y de gestión detectadas en la Facultad de Ciencias Económicas de la Universidad Nacional del Litoral, en Argentina. Las problemáticas institucionales se asociaban a la comunicación del estamento estudiantil, a la obtención sistematizada de información referida al contenido y tipo de consultas realizadas por los alumnos y al tiempo y tipo de respuestas ofrecidas, entre otras cuestiones.Cabe destacar que, para una Facultad que debe dar respuesta a las necesidades y requerimientos de más de cinco mil alumnos, estas deficiencias se tornaban críticas. Es así que, con el fin de mejorar la comunicación institucional, se desarrolló, desde la misma Facultad, un software para un sistema online que usa tecnologías libres, intenta superar las limitaciones del correo electrónico y evita la saturación natural de las líneas telefónicas. La creación del SICA permite que varios agentes puedan responder las consultas de los estudiantes al mismo tiempo, evita que se conteste dos veces la misma pregunta, identifica internamente qué agente responde la consulta y homogeniza las respuestas. El sistema también permite realizar mediciones de control. Por ejemplo, medir la demora de las soluciones brindadas a los problemas planteados y clasificar los mensajes de acuerdo a diversas categorías. Así, la información que brinda el SICA es transformada, a su vez, en conocimiento para la toma de decisiones por parte de la gestión.","palabras_clave":"Tecnología educacional|gestión universitaria|política universitaria|cambio cultural"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1342,"idioma_id":4,"titulo":"Architecture of a Single-Chip 50 Gb\/s DP-QPSK\/BPSK Transceiver With Electronic Dispersion Compensation for Coherent Optical Channels","resumen":"The architecture of a single-chip dual-polarization QPSK\/BPSK 50 Gigabits per second (Gb\/s) DSP-based transceiver for coherent optical communications is presented. The receiver compensates the chromatic dispersion (CD) of more than 3,500 km of standard single-mode fiber using a frequency-domain equalizer. A time-domain four-dimensional MIMO transversal equalizer compensates up to 200 ps of differential group delay (DGD) and 8000 ps 2 of second-order polarization-mode dispersion (SOPMD). Other key DSP functions of the receiver include carrier and timing recovery, automatic gain control, channel diagnostics, etc. A novel low-latency parallel-processing carrier recovery implementation which is robust in the presence of laser phase noise and frequency jitter is proposed. The chip integrates the transmitter, receiver, framer and host interface functions and features a 4-channel 25 Gs\/s 6-bit ADC with a figure of merit (FOM) of 0.4 pJ\/conversion. Each ADC channel is based on an 8-way interleaved flash architecture. The DSP uses a 16-way parallel processing architecture. Extensive measurement results are presented which confirm the design targets. Measured optical signal-to-noise ratio (OSNR) penalty when compensating 200 ps DGD and 8000 ps 2 is 0.1 dB, while OSNR penalty when compensating 55 ns\/nm CD (corresponding to 3,500 km of standard single-mode fiber) is 0.5 dB.","palabras_clave":"COHERENT|OPTICAL|COMMUNICATIONS|DSP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1343,"idioma_id":5,"titulo":"Modelación del impacto  hidrosedimentológico de los incendios forestales","resumen":"Se presentan los resultados de las simulaciones realizadas con el modelo TREX en la cuenca del río de La Suela (provincia de Córdoba) con el objetivo de analizar el impacto de la ocurrencia de incendios en los hidrogramas y sedimentogramas de descarga de la cuenca, luego del primer episodio lluvioso post-incendio. TREX es un modelo hidrológico distribuido (modelo de celdas) orientado a la simulación de eventos que puede representar diversos procesos hidrológicos y la producción, transporte y deposición de sedimentos. Se analizó la dependencia de la variación porcentual en las magnitudes de los picos del hidrograma y del concentrograma, en función de dos índices de base heurística, observándose una mejor correlación en la respuesta líquida. Se concluye que el modelo TREX permite obtener información valiosa para analizar la respuesta de las cuencas serranas de Córdoba ante los eventos ígneos que frecuentemente las azotan.","palabras_clave":"Incendios Forestales|Modelación hidrológica|Sedimentos|Sierras de Córdoba"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1344,"idioma_id":4,"titulo":"Agents that learn how to generate arguments from other agents","resumen":"Learning how to argue is a key ability for a negotiator agent. In this paper, we propose an approach that allows agents to learn how to build arguments by observing how other agents argue in a negotiation context. Particularly, our approach enables the agent to infer the rulesfor argument generation that other agents apply to build their arguments. To carry out this goal, the agent stores the arguments uttered by other agents and the facts of the negotiation context where each argument is uttered. Then, an algorithm for fuzzy generalized association rules is applied to discover the desired rules. This kind of algorithm allows us (a) to obtain general rules that can be applied to dierent negotiation contexts; and (b) to deal with the uncertainty about the knowledge of what facts of the context are taken into account by the agents. The experimental results showed that it is possible to infer argument generation rules from a reduced number of observed arguments.","palabras_clave":"INTELLIGENT AGENTS|ARGUMENTATION-BASED NEGOTIATION|FUZZY GENERALIZED ASSOCIATION RULES|LEARNING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1345,"idioma_id":5,"titulo":"ANÁLISIS MULTIFRACTAL DE LA SERIE TEMPORAL DE CAUDALES DIARIOS EN EL RÍO NEUQUÉN","resumen":"En el presente estudio se aplica el Análisis Multifractal (AM) para   analizar la variabilidad del patrón de comportamiento del caudal medio   diario en el río Neuquén, registrado en la estación Paso de Indios por   un período de cien años (1911-2011). El AM describe la invariancia de   las propiedades de escala de series temporales. El espectro multifractal   (EM) es una función de los exponentes de Hölder del sistema, los cuales   caracterizan el grado de irregularidad de la señal, y sus parámetros   más significativos son la ?huella dactilar? del proceso. Existen   diversas variantes para calcular este espectro, siendo el espectro de Legendre o termodinámico, fL,   el más utilizado en las aplicaciones. Se analizan los datos de la serie   completa y de distintas subseries, dividiéndolos por décadas y por   períodos de veinte años. Se calcula fL, para todas ellas y, apartándose del AM estándar, también se calcula otro espectro llamado espectro por-definición, fD, el cual ofrece una descripción más geométrica de la estructura de singularidad. Los parámetros de fD y fL   son comparados con la variación de los exponentes de Hurst de las   series, y de un índice de predictibilidad climática. Los resultados   obtenidos muestran la presencia de cambios cualitativos en el patrón de   comportamiento del régimen de caudales, sobre todo en la última década   del siglo XX.","palabras_clave":"Espectros Multifractales|Caudales Medios Diarios|Exponentes de Hölder|Bimultifractalidad|Índice de Predictibilidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1346,"idioma_id":5,"titulo":"Incidencia de la proporcion de maiz sobre la degradabilidad in situ de heno de alfalfa en dietas para caprinos","resumen":"El objetivo del presetne trabajo fue evaluar la incidencia de la proporcion de maiz sobre la degradabilidad in situ de la materia seca&nbsp; y de las fracciones de la pared celular y el pH ruminal","palabras_clave":"CABRAS|MAÍZ|PH|FIBRA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1348,"idioma_id":4,"titulo":"Analysing Prosody and Meaning in Spanish: Implications for the Teaching of English as a Foreign Language","resumen":"The aim of this work is to identify the difficulties that Spanish learners of English may have when confronted with the L2 intonation. The different tone choices made by native speakers of Spanish in North Patagonia, Argentina, are analysed in the light of the Autosegmental-Metrical framework (LADD, 1996) and Relevance Theory (SPERBER AND WILSON, 1995, 2004). The findings are compared with the tone choices produced by native speakers of English.  The main results indicate that the same procedural instruction is manifested in both languages by means of L* L% and H+L* L%. However, Spanish favours L* whereas English prefers H+L*.","palabras_clave":"PROSODY|MEANING|TEACHING ENGLISH AS A FOREIGN LANGUAGE|SPANISH LEARNERS OF ENGLISH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1349,"idioma_id":5,"titulo":"Atlas de Anatomía Vegetal. Tejidos y órganos vegetativos","resumen":"El presente Atlas ilustra los tejidos y la anatomía de los órganos vegetativos de Plantas Vasculares.Consta de los siguientes 11 temas: epidermis, tejidos fundamentales y vasculares, meristemas,estructura primaria y secundaria de raíz y tallo, hoja, leño y estructuras secretoras. Se realizó en elLaboratorio de la Cátedra de Morfología Vegetal, Escuela de Biología, Facultad de Ciencias Exactas,Físicas y Naturales, Universidad Nacional de Córdoba. Está destinado a Profesores y Alumnos deCiencias Biológicas e interesados en el conocimiento de la estructura interna de las plantas.","palabras_clave":"ATLAS|TEJIDOS|RAÍZ|TALLO|HOJA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1351,"idioma_id":5,"titulo":"El taller textil como excepción: tres argumentos para su (in)visibilidad","resumen":"El taller textil en Argentina no es un espacio de trabajo usual: es a la vez tallerdormitorio  y espacio ?comunitario?, de una intensidad laboral que se extiende en  jornadas de más de doce horas, con turnos rotativos, y que se combina con una  apuesta migratoria de gran riesgo. En esos pocos metros transcurre la vida completa de  muchos migrantes recién llegados de distintos lugares de Bolivia, en la medida que el  taller soluciona en un mismo tiempo-espacio la cuestión habitacional y laboral. Allí se  cocina, se cría a los niños, se duerme y se trabaja y, al principio, es el modo de  protegerse de una ciudad que se desconoce. Aquí nos proponemos enlazar las secuencias de su (in)visibilización para ver en funcionamiento las estrategias de victimización, moralización y judicialización que organizan un determinado campo de visibilidad de la cuestión del trabajo migrante en Argentina y,  más amplio aún, para sugerir un tipo de vinculación entre norma y economía popular,  que tiene como efecto la moralización (y condena) del mundo de los pobres.","palabras_clave":"TRABAJO|MIGRANTES|VISIBILIZACION|MORALIZACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1352,"idioma_id":4,"titulo":"Lattice-Boltzmann Method with immersed boundary conditions for fluid simulations of multiples species","resumen":"An algorithm to simulate multiples mixtures in single phase with different molecular weights is presented. The coupling of multiple mixtures with the immerse boundary method is proposed. This algorithm is designed to model problems of multiple mixtures using Lattice Boltzmann method which in turn can interact with complex objects modeled with immersed boundaries. The Lattice Boltzmann method is derived from kinetic theory by discretizing multiples fluid Boltzmann equations in which cross-collisions and self-collisions are treated independently. The method is validated against analytical solution in diffusion of binary mixtures and evaluated in ternary mixtures in a curved channel.","palabras_clave":"multiple species|lattice boltzmann|inmersed boundary|curved boundary"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1354,"idioma_id":5,"titulo":"Niña o niño: intervalos entre nacimientos y el sexo de los hijos entre los Wichi","resumen":"El intervalo intergenésico es, desde  una perspectiva evolutiva, medida de la inversión parental en un hijo. Los  padres toman activamente decisiones con respecto a la longitud de los  intervalos entre nacimientos, aún en sociedades sin contraconcepción. Esta  inversión parental puede variar según el sexo del hijo. Las preferencias  sexuales varían en las sociedades con las limitaciones ecológicas que estas  afrontan. Las sociedades tradicionales basadas en la subsistencia exhiben una  fertilidad cercana a la natural y tienen bajos niveles de acceso al cuidado de  salud moderno. Se investigan los intervalos entre partos en las madres Wichí  para estimar los tiempos que insume el esfuerzo maternal en cada descendiente y  si hay diferencias sexuales en la regulación temporal de los nacimientos. Las  madres espaciaron sus partos en intervalos medios de 38,05 meses. El 60,8% cumplieron  intervalos menores de 36 meses. La proporción sexual secundaria resultó sesgada  hacia las mujeres. Sin embargo, los intervalos abiertos por varón resultaron  significativamente más largos que los abiertos por mujer. No se detectó efecto  sexual para el cierre. La estrategia  reproductora Wichí está dirigida a asegurar una mayor inversión maternal en los  hijos varones. Ya sea porque buscan por decisión conciente la supervivencia del  sexo más escaso que, justamente por esta razón, aumenta su valor reproductivo,  o porque el intervalo entre nacimientos está ajustado biológicamente y las  madres tardan más tiempo en recobrar algún nivel de condición fisiológica  necesaria para un próximo embarazo. Estos mecanismos no son mutuamente  excluyentes y pueden ambos estar operando a la vez.","palabras_clave":"INTERVALO INTERGENESICO|INVERSION PARENTAL|PROPORCION SEXUAL|WICHI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1355,"idioma_id":4,"titulo":"Effects of environmental temperature on the onset and the duration of oviposition period of Caiman latirostris","resumen":"Abstract.- Reproductive  biology of Broad-snouted Caiman (Caiman  latirostris) may be affected by climatic variables. &nbsp;However, it remains unknown what specific  climatic variables affect the onset of oviposition as well as its duration. In  this study, we use a serie of climatic data of the preceding four weeks to know which of them influence these reproductive characteristics, and we observed the weather conditions  of the immediate week prior to oviposition being the most important factor. &nbsp;We found a delay in  the onset of oviposition when the  number of days with  temperatures below 20°C  in the previous weeks is higher. &nbsp;Conversely, we found that oviposition starts  earlier in a season when the number of days with temperatures above 33°C during the previous week increases. &nbsp;Additionally, the duration of the oviposition  period is longer when the number with temperatures above 33°C increases. No relationship was found between the onset of oviposition and the number of storms or the amount of rainfall in the four preceding  weeks. &nbsp;We also  noted that in seasons when the start oviposition is delayed, the duration of  oviposition of this period is shorter. &nbsp;The onset of  oviposition of female C. latirostris  may vary up to three weeks among years. &nbsp;The  information obtained is a useful tool for managing strategies because it  predicts the timing and duration of oviposition based on climatic information.","palabras_clave":"Broad-snouted Caiman|climate variables|clutch|eggs|nesting onset"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1356,"idioma_id":4,"titulo":"Proteolytic activity of Oenococcus oeni enables the increase in antioxidant and antihypertensive activities from wine","resumen":"Oenococcus oeni is a lactic acid bacterium involved in winemaking where it generally carries out the malolactic fermentation converting the wine´s malic acid into lactic acid.                                   In this work were used the strain m of Oenococcus oeni. The culture was inoculated at 108 Log CFU\/mL in a synthetic wine medium (SW) supplemented with a fraction of high molecular weight constituted by proteins and polypeptides (FPP) obtained from Cabernet Sauvignon and Syrah wines from Colalao del Valle, Tucumán, Argentine. In presence of FPP, O. oeni maintains viability after 48 h incubation time and release an extracellular proteolytic activity. Therefore, a release of peptides of 1.247 and 1.373 mg N\/L at 48 h of incubation time was detected in SW supplemented with FPP from Cabernet Sauvignon and Syrah wines respectively. Concomitantly with the maximum peptide release, the ?in vitro? biological activities were increased. The released peptides from Cabernet Sauvignon wine enables the increase in the ferric reducing antioxidant power (FRAP) capacity, the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the inhibition of angiotensin I-converting enzyme (ACEI) activity in 392.8 µmol FeSO4\/L, 9.7 % and 63.9 %, respectively. In presence of FPP of Syrah wine, the released peptides increases in 156.5 µmol FeSO4\/L, 5.5 % and 13.8 % the FRAP, DPPH and ACEI activities, respectively. The utilization of Oenococcus oeni m to carry out the malolactic fermentation would contribute to enhance the beneficial biological activities of the final product and provide an additional value to regional wines.                                   ","palabras_clave":"Antihypertensive activity|Antioxidant activity|Bioactive peptides|Oenococcus oeni"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1357,"idioma_id":4,"titulo":"Psycho-social factors influencing forest conservation intentions on the agricultural frontier","resumen":"Abstract  Remnant forest fragments are critical to conserve biological diversity yet these are lost rapidly in areas under agricultural expansion. Conservation planning and policy require a deeper understanding of the psycho-social factors influencing landholders? intentions towards conserving forest fragments. We surveyed 89 landholders in an agricultural frontier of the South American Gran Chaco and employed survey data to test three social psychological models: the Theory of Planned Behavior (TPB) and two modified versions of it, one integrated to the Norm Activation Theory (TPB-NAT) and one including the effect of identity (TPB-NAT-Identity). The TPB was the most parsimonious model and explained a large variance of conservation intentions (41%). Social norms and attitudes had the largest direct influence on intentions across the three models, and identity had a significant role in shaping social norms and attitudes. Interventions aimed at building social capital within landholder networks provide the best hope for influencing pro-conservation norms.","palabras_clave":"AGRICULTURAL FRONTIER|DRY FORESTS|CONSERVATION BEHAVIOR|GRAN CHACO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1359,"idioma_id":5,"titulo":"MORFODINÁMICA  DE LAS DUNAS COSTERAS DEL CABO SAN ANTONIO, PROVINCIA DE BUENOS AIRES, ARGENTINA","resumen":"Las dunas costeras son reconocidas en la mayoría de las costas del mundo, pero son más comunes en las costas disipativas, como las del Cabo San Antonio, provincia de Buenos Aires, donde la baja pendiente de las playas, permite el  desarrollo de áreas de generación extensas que proveen condiciones óptimas para la formación de dunas costeras. Las dunas costeras del Cabo San Antonio, se desarrollan en una faja paralela a la costa sin una morfología definida, con anchos que varia entre 20 y 70m. Las alturas oscilan entre escasos metros a 10m aproximadamente. Las pendientes del sector costero varían entre 3 y 7 º y del sector continental de 7 a 18 º.   Los 7 sitios de muestreo utilizados para caracterizar la sedimentología de las dunas costeras presentaron condiciones unimodales, con valores de asimetría casi simétricos o levemente negativos y selección entre buena y muy buena. Los valores de media se dan en un rango de entre 2 y 2,72 Ø, las distribuciones acumulativas no presentan truncamientos internos y más del 80% de las  partículas son transportadas por saltación.  Se diferenciaron dos categorías de dunas costeras, las naturales y las degradadas. Del primer grupo  se reconocen las  dunas  incipientes, formadas a partir del establecimiento de las primeras plantas vasculares (Spartina coarctata  y Panicum racemosum) y las dunas costeras propiamente, en las cuales los factores físicos como es el viento, humedad y disponibilidad de sedimentos tienen mayor incidencia en la dinámica de su desarrollo.   La urbanización a la que se sometió el litoral del Cabo San Antonio desde 1935 generó una rápida degradación, que para 1984 ya modificaba sustancialmente el 50% del cordón de dunas costeras. Se determinó la tendencia de la velocidad de degradación del cordón costero, de aproximadamente 650 m.a-1. De mantenerse esta tendencia para mediados de la década del 2030, no existirán sectores relativamente importantes del cordón costero natural.   La acción de fijación de la duna costera con especies alóctonas, así también como el arrasamiento total o parcial, para el trazado de avenidas costaneras o construcción de edificios, casas o balnearios, provoca alteraciones y tiene una relación directa con el aumento de la vulnerabilidad a la erosión marina del sector costero.","palabras_clave":"duna costera |erosión |hidrodinámica costera"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1360,"idioma_id":5,"titulo":"Mímesis y pólis: sobre el silencio de Aristóteles y el gesto político de su Poética","resumen":"El objetivo principal de este trabajo es revisar la relación entre mímesis y pólis a partir de las consideraciones de la Poética de Aristóteles. En tal sentido, atenderé en primer lugar a los argumentos que se esgrimen en torno a los silencios de dicha obra y que conducirían a una eventual apoliticidad. En contra de esta posición, pretendo mostrar que en la Poética, Aristóteles sugiere un gesto político, al enfatizar el aprendizaje mimético del cual se pueden inferirse implicancias para la vida en la pólis.","palabras_clave":"ARISTOTELES|MIMESIS|POLIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1361,"idioma_id":4,"titulo":"An Analysis of Intercultural Bilingual Education in Argentina","resumen":"Purpose ? The purpose of this article is to assess the state of affairs of intercultural bilingual education (IBE) policies in Argentina. IBE encompasses educational proposals designed to address the specific needs of ethnolinguistic minorities.Design\/methodology\/approach ? The article is divided into three parts and methodologically analyzes different types of materials (national legislation, bibliography and personal research experiences).Findings ? In the first section, the educational policies on cultural and linguistic diversity are contextualized historically. Later, IBE is defined considering five main characteristics (the neoliberal compensatory education policy, educational decentralization\/regionalization, the educational category of the system, linguistic goals and teacher profiles). Finally, the conclusions list the challenges, goals that have yet to be achieved and the future perspectives of this educational policy.Originality\/value ? This article contributes to the field of studies on diversity in the schooling of ethnically different groups.","palabras_clave":"EDUCATIONAL POLICY|DIVERSITY|REGIONALIZATION|INEQUALITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1362,"idioma_id":5,"titulo":"Estadios embrionarios post-oviposición de Atractus reticulatus (Serpentes: Dipsadidae)","resumen":"GHJHDHFHF","palabras_clave":"SERPENTES|OVIPARIDAD|ESTADIOS DEL DESARROLLO|CARACTERES EMBRIONARIOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1363,"idioma_id":5,"titulo":"Vergüenza como empoderamiento. El caso de los crímenes sexuales de lesa humanidad.?.","resumen":"            El concepto de victimización ha sido analizado en oposición  radical al de agencia: la pasividad de la herida frente a la potencia de la  capacidad de acción. Se ha dicho que la ?identidad herida? (Brown) implica un  desprecio a las posibilidades transformativas y un sometimiento al  esencialismo. El objetivo  de este trabajo consiste en desafiar esta dicotomía a través del análisis de  los testimonios prestados en los juicios sobre violencia sexual en tanto crímenes  de lesa humanidad gracias a un análisis de los afectos involucrados (Ahmed,  Ngai), muy especialmente en lo que hace al rol de la vergüenza (Sedgwick).  Tomando como marco  referencial el giro afectivo, y a través del análisis de metatestimonios recogidos especialmente para este trabajo relativos a la experiencia de dar testimonio en  los juicios por crímenes de lesa humanidad en la Argentina por  mujeres que sufrieron violencia sexual surge justamente  la posibilidad de desafiar la dicotomía en cuestión y revisar ciertas  concepciones sobre estos testimonios (Jelin, 2012). Muy especialmente a partir  del reconocimiento de esas violaciones como delitos de lesa humanidad por sí mismas (Sondereguer, 2010).       &lt;!--   \/* Font Definitions *\/  @font-face  \t{font-family:Calibri;  \tpanose-1:2 15 5 2 2 2 4 3 2 4;  \tmso-font-charset:0;  \tmso-generic-font-family:auto;  \tmso-font-pitch:variable;  \tmso-font-signature:-520092929 1073786111 9 0 415 0;}  @font-face  \t{font-family:Cambria;  \tpanose-1:2 4 5 3 5 4 6 3 2 4;  \tmso-font-charset:0;  \tmso-generic-font-family:auto;  \tmso-font-pitch:variable;  \tmso-font-signature:-536870145 1073743103 0 0 415 0;}  @font-face  \t{font-family:'MS Mincho';  \tmso-font-alt:'&#65325;&#65331; &#26126;&#26397;';  \tmso-font-charset:128;  \tmso-generic-font-family:modern;  \tmso-font-pitch:fixed;  \tmso-font-signature:-536870145 1791491579 18 0 131231 0;}   \/* Style Definitions *\/  p.MsoNormal, li.MsoNormal, div.MsoNormal  \t{mso-style-unhide:no;  \tmso-style-qformat:yes;  \tmso-style-parent:'';  \tmargin:0in;  \tmargin-bottom:.0001pt;  \tmso-pagination:widow-orphan;  \tfont-size:12.0pt;  \tfont-family:Cambria;  \tmso-fareast-font-family:'MS Mincho';  \tmso-bidi-font-family:'Times New Roman';  \tmso-ansi-language:EN-US;}  .MsoChpDefault  \t{mso-style-type:export-only;  \tmso-default-props:yes;  \tfont-size:10.0pt;  \tmso-ansi-font-size:10.0pt;  \tmso-bidi-font-size:10.0pt;  \tfont-family:Cambria;  \tmso-ascii-font-family:Cambria;  \tmso-fareast-font-family:'MS Mincho';  \tmso-hansi-font-family:Cambria;}  @page WordSection1  \t{size:8.5in 11.0in;  \tmargin:70.85pt 85.05pt 70.85pt 85.05pt;  \tmso-header-margin:.5in;  \tmso-footer-margin:.5in;  \tmso-paper-source:0;}  div.WordSection1  \t{page:WordSection1;}  --&gt;","palabras_clave":"afectos|género|testimonio|juicios"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1364,"idioma_id":4,"titulo":"RECRUITMENT  OF  GABAA  RECEPTORS  AND  FEARFULNESS  IN  CHICKS:  MODULATION BY SYSTEMIC INSULIN AND\/OR EPINEPHRINE","resumen":"One-day-old chicks were individually assessed on their latency to peck pebbles, and categorized as low latency (LL) or high latency (HL) according to fear. Interactions between acute stress and systemic insulin and epinephrine on GABAA receptor density in the forebrain were studied. At 10 days of life, LL and HL chicks were intraperitoneally injected with insulin, epinephrine or saline, and immediately after stressed by partial water immersion for 15 min and killed by decapitation. Forebrains were dissected and the GABAA receptor density was measured ex vivo by the 3[H]-flunitrazepam binding assay in synaptosomes. In non-stressed chicks, insulin (non-hypoglycemic dose) at 2.50 IU\/kg of body weight incremented the Bmax by 40.53% in the HL chicks compared to saline group whereas no significant differences were observed between individuals in the LL subpopulation. Additionally, insulin increased the Bmax (23.48%) in the HL group respect to the LL ones, indicating that the insulin responses were different according to the anxiety of each category. Epinephrine administration (0.25 and 0.50 mg\/kg) incremented the Bmax in non-stressed chicks, in the LL group by about 37% and 33%, respectively, compared to ones injected with saline. In the stressed chicks, 0.25 mg\/kg bw epinephrine increased the Bmax significantly in the HL group by about 24% compared to saline, suggesting that the effect of epinephrine was only observed in the HL group under acute stress conditions. Similarly, the same epinephrine doses co-administered with insulin increased the receptor density in both subpopulations and also showed that the highest dose of epinephrine did not further increase the maximum density of GABAAR in HL chicks. These results suggest that systemic epinephrine, perhaps by evoking central norepinephrine release, modulated the increase in the forebrain GABAA receptor recruitment induced by both insulin and stress in different ways depending on the subpopulation fearfulness.","palabras_clave":"EPINEPHRINE|INSULIN |CATEGORIZATION|PARTIAL WATER STRESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1365,"idioma_id":4,"titulo":"Fatty Acids in the Meat of Buffaloes Supplemented with Fish Oil","resumen":"The purpose of this study has been to investigate the influence of both a supplementary fish oil diet on conjugated linoleic acid (CLA) and n6 and n3 fatty acids on intramuscular fat in Mediterranean buffalo meat. Twenty animals were randomly divided into two groups and fed with Brachiaria brizantha, 3Kg rice bran, 500 g corn and 500 g sunflower pellets for 60 days. Group I received this diet only while in group II each animal received additional 100 ml fish oil daily. Results indicated a significant decrease of palmitic fatty acid in group II (232.67 mg\/g fat) in relation to group I (254.73 mg\/g fat). Among unsaturated acids (AGI), the 9c 11t CLA value of group II (21.23 mg\/g fat) showed an increasein relation to group I (15.80 mg\/g fat), while the linoleic acid content of group II (28,85 mg\/g fat) decreased significantly in relation to group I (47,00 mg\/g fat). However, the alpha linolenic acid showed no significant difference between the supplemented diet group and the control group (10.31 and 10.70 mg\/g fat, respectively). Group II n6\/n3 ratio wasnarrower (2.69:1) than that of group I (4.55:1). Summing up, group II diet, which included fish oil, increased the CLA content in intramuscular fat and decreased the n6 fatty acids, improving the n6\/n3 ratio.","palabras_clave":"BUFFALO|CLA|n6 y n3|FISH OIL|MEAT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1366,"idioma_id":4,"titulo":"Resonance states in a cylindrical quantum dot with an external magnetic field","resumen":"Bound and resonance states of quantum dots play a significant role in  photo-absorption processes. In this work, we analyze a cylindrical quantum dot,  its spectrum and,   in particular, the behaviour of the lowest resonance state when a magnetic field  is applied along the symmetry axis of the cylinder. To obtain the energy and  width of the resonance we use the complex rotation method. As it is expected  the structure of the spectrum is strongly influenced by the Landau levels  associated to the magnetic field. We show how this structure affects the  behaviour of the resonance state and that the binding of the resonance has a  clear interpretation in terms of the Landau levels and the probability of  localization of the resonance state. The localization probability and the  fidelity of the lowest energy state allow to identify two different  physical regimes, a large field-small quantum dot radius regime and a small  field-large quantum dot radius, where the binding of the resonance is  dominated by the field strength or the potential well, respectively.","palabras_clave":"resonances|fidelity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1368,"idioma_id":5,"titulo":"Redefiniciones del mérito en secundaria: el lugar del esfuerzo en las Escuelas de Reingreso de la Ciudad de Buenos Aires","resumen":"El presente artículo aborda la redefinición del mérito en un conjunto de escuelas de la Ciudad de Buenos Aires, conocidas como Escuelas de Reingreso, resultado de una política de inclusión en la escuela secundaria de jóvenes que han quedado fuera del sistema en su mayoría provenientes de sectores populares con responsabilidades laborales y\/o familiares tempranas. A partir del análisis de las entrevistas realizadas a los equipos docentes y estudiantes, se reconstruyen los rasgos que van definiendo lo meritorio en estas instituciones, delineando así un patrón de alumno esperable. El esfuerzo de los estudiantes cobra centralidad, pero no sólo aquel relacionado con las tareas estrictamente escolares, sino particularmente el esfuerzo extraescolar que los estudiantes realizan a fin de sostener su escolarización, el cual se vuelve parcialmente canjeable en términos académicos. Esta redefinición no está exenta de tensiones, ya que cobra centralidad en la definición de quienes logran permanecer en estas escuelas.    La revista está indexada en: DIALNET; Red de Revista Científicas de América Latina y El Caribe, España y Portugal (REDALYC); Latindex, IRESIE; DOAJ (Directory of Open Access Joural), OEI - Biblioteca Digital; e-Revistas.","palabras_clave":"Educación Media|Argentina|Meritocracia|Escuelas de Reingreso"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1369,"idioma_id":4,"titulo":"THE OVARY OF CHINCHILLA LANIGERA IN CAPTIVITY","resumen":"Chinchilla is one of the most valuable rodents in the fur industry, especially the lanigera variety.&nbsp; An important problem of the intensive breeding of chinchillas is their low reproductive rate, so that they are an interesting species to study the reproductive physiology of the females in captivity.                                   The morphology of chinchilla ovary agrees for the most part with the description made for other South American hystricognath rodents, especially as regards anatomy and, to a lesser degree, histology.                                   The presence of numerous primary follicles throughout the annual cycle show that they constitute the follicular reserve and that a small number of follicles are recruited to initiate growth and differentiation during folliculogenesis.&nbsp; The females usually have one, and exceptionally two, embryos that survive to term                                   Some primary follicles with two or more oocytes were found; Such polyovular follicles were not found in the other follicular development stages, suggesting that they do not develop or are eliminated.                                   In chinchilla, we can observe only one or two big corpora lutea (CL) and three or five small corpora lutea or accessory CL. The presence of these accessories CL might reflect the importance of the maintenance of continuous hormonal production to support prolonged gestation.                                   In chinchilla, no corpora albicans were observed. However, atretic CL was also present, showing signs of degeneration in luteal cells.                                   The interstitial cells distributed throughout the cortex was the main histological feature in comparison with previous reports in other species                                   &nbsp; Antral atresia was observed in all sizes of antral follicles, basal atresia was confined exclusively to smaller follicles.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;                               ","palabras_clave":"OVARY|HISTOLOGY|CHINCHILLA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1371,"idioma_id":4,"titulo":"INCIDENCE OF FUNGIC PATHOGEN IN GRAINS OF MAIZE FROM A FERTILIZATION NITRO-SULPHURED CROP","resumen":"The objectof the present work was to determine the incidence of fungic pathogenic ingrains of two cultivars of maize, with different levels of nitro-sulphuredfertilization. The cultivars used were ACA 2000 and ACA 417 RR2. On 2012 thetreatments consisted of an arrangement factorial of three levels of N (N0:control; N75: 75 kg N.ha-1 and N150: 150 kg N.ha-1) andtwo levels of S (S0: 0 kg S ha-1 and S40: 40 kg S. ha-1).To determine the fungic incidence the grains incubated on 2 %APG with lightalternation (ISTA, 2009). A total of 200 seeds \/ hybrids were analyzed. Thepercentage of each pathogen were determined. The statistical was in BCAD with 3repetitions \/ hybrids. The information was analyzed by ANOVA and Duncan. Thepathogens present were Aspergillus flavus, Diplodia spp., Fusarium spp. andPenicillium spp. The incidence of Aspergillus flavus was low (0-3%) did notpresent differences between levels of N, level of S, and inteacción NxS.Diplodia were no significant differences, the values were low (0-3%). ForFusarium graminearum the incidence values were no differences (15-50%). Thevalues of Fusarium moniliforme were of 0 to 3%. Penicillium with values on 50%to 90% showed differences between levels (p&lt;0,05) only on ACA 2000. Thehighest values on percentage were on N150- S0 y N0-S40","palabras_clave":"MAIZE|CULTIVAR|FERTILIZATION|FUNGIC PATHOGENIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1372,"idioma_id":5,"titulo":"PREFERENCIAS ALIMENTICIAS EN LAS MIELES INMADURAS DE Apis mellifera EN EL CHACO SERRANO (JUJUY, ARGENTINA)","resumen":"Este trabajo forma parte del estudio de los recursos alimentarios utilizados por Apis mellifera en el Distrito Chaco Serrano en la Provincia de Jujuy. Se presentan los resultados obtenidos en los análisis melisopalinológicos realizados a muestras de mieles inmaduras tomadas a lo largo de un ciclo productivo en dos apiarios: Finca Doña Hermes, (El Pongo) y Apiario Watraymiski, (Severino). Las muestras se procesaron de acuerdo a la metodología estándar utilizada en melisopalinología. Para El Pongo se registraron 62 tipos polínicos mientras que para Severino se identificaron 64. Se destacan los elementos de los grupos de Transición y los Antrópico, encontrándose como Dominantes Mimosa xanthocentra, Parapiptadenia excelsa, Salix humboldtiana, Rapistrum rugosum y Anadenanthera colubrina var. cebil.","palabras_clave":"Melisopalinología|Polen |Yungas |Transición"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1373,"idioma_id":4,"titulo":"An apomictic tetraploid Paspalum chaseanum cytotype and its cytogenetic relationship with P. plicatulum (Poaceae): taxonomic and genetic implications","resumen":"Abstract. Paspalum chaseanum Parodi (Poaceae) is a rare species seldom found in the vast phytogeographic Chaco regionof South America. It occurs in some localities as a diploid with 20 somatic chromosomes, reproduces sexually and is selfincompatible.A tetraploid cytotype was recently collected in this geographic region. This accession was determined toreproduce of aposporous apomixis and was crossed, as pollen donor, onto a sexual autotetraploid plant of P. plicatulumMichx. The meiotic chromosome pairing behaviour of both parents and their hybrids was primarily as bivalents andquadrivalents, indicating that tetraploid P. chaseanum is likely to have an autoploid origin, and that both speciesshare basically the same genome. Although some controversies exist regarding the subgeneric taxonomic classificationof P. chaseanum, these results support its inclusion in the informal Plicatula group of Paspalum. TheP. plicatulumP. chaseanum hybrids segregated for apomixis. The amount of seed set in some hybrids (up to 17%)and the presence of sexual as well as facultative apomictic individuals in the progeny suggest that gene transfer throughhybridisation is a feasible tool in genetic-improvement programs concerning these forage grass species.","palabras_clave":"APOMIXIS|INTERSPECIFIC HYBRID|PLICATULA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1375,"idioma_id":5,"titulo":"La planificacion hidrica en el contexto de la ecohidrología","resumen":"Se desarrolla la actual visión de la planificación esencia de la gestión integrada del recurso hídrico (GIRH) como el proceso sistemático para el conocimiento, gestión, desarrollo&nbsp;sostenible y supervisión del agua en el contexto de objetivos sociales, económicos y ambientales de manera equitativa, sin comprometer la sostenibilidad de los ecosistemas y considerando la necesaria participación de los interesados","palabras_clave":"Agua|Planificacion|Sustentabilidad|Ecohidrología"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1377,"idioma_id":5,"titulo":"los clubes de barrio: Deporte social y recreacion en un espacio de inclusion social","resumen":"Este trabajo, es un primer análisis sobre el desarrollo de los clubes de barrio en la Ciudad de La Plata, Buenos Aires, Argentina. En este artículo en particular se busca construir un  primer cuadro de situación sobre estas instituciones, su desarrollo histórico y sus ofertas actuales en el campo deportivo y de la recreación.","palabras_clave":"POLÍTICAS DEPORTIVAS|INCLUSIÓN SOCIAL|CLUB DE BARRIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1378,"idioma_id":5,"titulo":"Siembra directa: evaluación de la densidad aparente como indicador de la necesidad de labores de descompactación.","resumen":"La siembra directa (SD) ha sido reconocida como una alternativa de menor impacto ambiental que otros sistemas de cultivo. Sin embargo, algunos autores alertan que puede generar degradación de algunas propiedades del suelo. Preocupados por esta situación se hizo un seguimiento de la evolución bianual de la densidad aparente como indicador de la calidad de los suelos de la región centro norte de Córdoba. Se observó un aumento de la densidad aparente y una disminución en el contenido de materia orgánica en lotes con SD continua con marcadas diferencias respecto a los sitios de Monte Natural (MN) estos cambios estuvieron acompañados por la presencia de estructura laminar resultante de la pérdida de porosidad estructural. El impacto físico de la SD deja abierto el planteo de la necesidad de realizar tareas de descompactación para lo cual la densidad aparente es un indicador sensible del momento y profundidad a la que debe realizarse.","palabras_clave":"Densidad Aparente|Siembra Directa|Suelo franco-limoso"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1379,"idioma_id":5,"titulo":"Peronismo y juventud en el Noroeste Argentino. Alberto Iturbe y la joven dirigencia política en la conformación del primer peronismo en Jujuy","resumen":"El presente artículo se inserta dentro del conjunto de estudios que analizan al primer peronismo en los espacios provinciales. Tiene como objetivo examinar el rol de la joven dirigencia del partido liderado por Miguel Tanco en los años formativos del peronismo. En esta tarea se presta atención a uno de sus miembros y primer gobernador justicialista de la provincia de Jujuy: Alberto Iturbe. Con ello se pretende profundizar una perspectiva esbozada en ciertos trabajos que a nivel nacional resaltan la actuación desarrollada por distintas figuras políticas que confluirían luego en el peronismo. Nuestra hipótesis sostiene que en el lapso previo al surgimiento de este movimiento político, estos personajes tuvieron un notable desempeño en la función estatal, especialmente Iturbe en el campo de la obra pública, siendo su labor de gran trascendencia dada las graves necesidades materiales que exhibía por entonces la provincia. Su intensa actividad en este ámbito y en el político partidario, habrían coadyuvado en buena medida a su designación como candidato a gobernador en representación de la fuerza política que apoyaba a Perón en la provincia.","palabras_clave":"DIRIGENCIA|JUVENTUD|PERONISMO|JUJUY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1381,"idioma_id":4,"titulo":"Toxicity of endosulfan on embryo-larval development of the South American toad, Rhinella arenarum.","resumen":"Endosulfan (ES) is a widely used pesticide despite its extreme toxicity on a variety of taxa and its worldwide banish. In this context, the aim of this study was to evaluate the acute and chronic toxic effects of ES on the embryo-larval development of the common South American toad, Rhinella arenarum. The results showed that lethal and sublethal effects increased with concentrations and exposure time. The sensitivity to ES significantly incremented toward the larval period, being the complete operculum (S.25) the most susceptible stage (504-h LC50=0.01 mg ES\/L; LC10=0.004 mg ES\/L). With respect to sublethal effects, ES caused morphological abnormalities such as delayed development, edema, gill malformations, cellular dissociation, reduced body size, as well as marked behavioral alterations. Our results also showed that larvae exposed to concentrations of 0.005 and 0.01 mg ES\/L completed the metamorphosis earlier than controls, but with reduced size and weight. The 240-h Teratogenic Index (TI) was 6.03. Considering that the Hazard Quotients (HQ) for chronic exposure were over the level of concern value (LOC), and toxicity parameters obtained in this study for Rhinella arenarum are lower than the levels of ES reported in the environment, this pesticide should be considered a potential risk for this species.","palabras_clave":"Developmental toxicity| Pesticides|Behavioral toxicology|Ecological risk assessment|Amphibians| Endosulfan"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1382,"idioma_id":5,"titulo":"HLB (Huanglongbing)","resumen":"HLB: desarrollo de la enfermedad e identificación del agente causal.","palabras_clave":"HLB|Bacteria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1383,"idioma_id":5,"titulo":"CONSIDERACIONES SOBRE LA IDENTIDAD Y DELIMITACIÓN DE CELTIS CHICHAPE (WEDD.) MIQ. (CELTIDACEAE)","resumen":"En la presente contribución se analizan los criterios usados por varios autores para interpretar la identidad de Celtis chichape (Wedd.) Miq. (Celtidaceae). Se considera que el propuesto por Dottori & Hunziker (1994) es el más adecuado y ajustado a las características naturales de estos arbustos.","palabras_clave":"Celtis chichape|Chaco|Celtis pallida var. discolor|Celtidaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1384,"idioma_id":5,"titulo":"NUEVA COMBINACIÓN EN CELTIS EHRENBERGIANA (KLOTZSCH) LIEBM.","resumen":"Dada la sinonimización de Celtis pallida Torr. bajo C. ehrenbergiana (Klotzsch) Liebm., lo cual incluye también a C. pallida var. discolor Hunz. & Dottori debido a sus claras diferencias con C. chichape (Wedd.) Miq., se propone una nueva combinación para este taxón.","palabras_clave":"Celtis ehrenbergiana|Celtidaceae|tala negro|tala pispo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1385,"idioma_id":4,"titulo":"Revision of two colorimetric methods to quantify glomalin related compounds in soils subjected to different managements","resumen":"The aim of this study was to analyze two colorimetric  methods used to determine easily extracted glomalinrelated  soil proteins (EE-GRSP). The historically and most  commonly used method for measurement of EE-GRSP as  total protein has been the Bradford assay. After some  troubles\/inconsistencies with this method, we carefully analyzed  the Bradford assay, measuring a dilution series of the  EE-GRSP fraction and analyzing the time stability of the  product. In addition, we did similar analysis of another colorimetric  method that quantifies total protein, the bicinchoninic  acid (BCA) assay. Unexpectedly, we found that the EE-GRSP  concentration values determined by Bradford assay were dependent  and variable with the dilution level of the soil extract;  moreover, the Bradford assay shows a great instability with  the time when soil samples were analyzed but not when  protein solution as bovine serum albumin (BSA) was used  as control. On the contrary, the BCA assay was independent of  the dilution levels of the soil extract and showed stability in  the time either for soil samples or BSA protein quantification.  These results were consistent and independent on the different  type of soils corresponding to different locations and with  different textures.","palabras_clave":"GRSP|BRADFORD ASSAY|BICINCHONINIC ACID (BCA) ASSAY|SOIL PROTEINS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1387,"idioma_id":5,"titulo":"La investigación de la institución de la educación en diferentes ámbitos. Aportes teórico-metodológicos de los enfoques institucionales usados en las investigaciones del Programa Instituciones educativas.","resumen":"El programa ?Instituciones educativas?, creado en el IICE en 1986, se ocupa del estudio de las instituciones educativas entendida en su realidad bifronte. Como organizaciones institucionales de diferente grado de complejidad y vastedad y como conjunto de rasgos que hacen del individuo un ser reconocido como sujeto de un mundo social que a su vez se reconoce por una identidad en la que esos rasgos estas presentes como sostén y referencia de adhesión o de oposición.  Conforma el marco conceptual de sus investigadores un campo amplio al que concurren las ciencias sociales, las ciencias psicológicas con importante presencia del psicoanálisis y las así llamadas posiciones institucionalistas.   Cada investigador tiene sus referencias ?preferidas? ?de este y otros campos disciplinarios- , aquellas que mejor le ayudan a explicar  los fenómenos que ocurren en su campo de investigación. El conjunto que conforma el Programa  encuentra útiles conceptualizaciones que se han ido fraguando en los procesos mismos de investigación por sus propias necesidades.  Seguramente en algún momento próximo trataremos de exponer al modo de un diccionario sui generis el conjunto de conceptos que cada investigador ha desarrollado en la lucha que significa procurar comprender, creer que se ha comprendido, poder decirlo sin reiterar lo que está dicho y logrando la palabra que alude a la realidad empírica que antes no tenía un nombre que la expresara del modo en que se la ha comprendido.","palabras_clave":"institucionalistas|investigación|intervención|teorías"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1388,"idioma_id":5,"titulo":"Crecimiento, distribución y composición de la población urbana y rural en el Chaco entre 1991 y 2010","resumen":"La información obtenida de los datos censales permite la indagación sobre cuestiones que resultan de fundamental importancia para conocer y analizar un territorio. Algunos de los principales fundamentos de estudio, obtenidos a partir de datos censales, se representan a través de la variación de población, el peso relativo de las unidades demográficas analizadas y su composición urbano-rural. Con ellos puede obtenerse una ?radiografía? de la situación demográfica de un territorio, que posibilita inferir tendencias y plantear interrogantes sobre la dinámica de la población analizada, con el objeto de contribuir a mejorar la toma de decisiones y la aplicación de políticas públicas","palabras_clave":"CRECIMIENTO|DISTRIBUCIÓN|COMPOSICIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1389,"idioma_id":5,"titulo":"LA INTEGRACIÓN A LA VIDA UNIVERSITARIA: UNA MIRADA DESDE EL INGRESANTE","resumen":"    El presente trabajo es un estudio descriptivo y  exploratorio sobre la integración del estudiante a la vida universitaria. Se  trata de un trabajo que ofrece&nbsp; un  análisis sobre el espacio de ingreso como agente socializador en el proceso de  formación de actitudes y valores&nbsp; en los  estudiantes que ingresan a la carrera de Psicología en la Universidad Nacional  de Tucumán.    El espacio de ingreso,&nbsp; se estructura alrededor de dos ejes: el de  ambientación, y el académico. Por un lado, se&nbsp;  posibilita la integración social, a través de las relaciones y vínculos  que se&nbsp; establece con compañeros y  Coordinadores y por otro lado,&nbsp; al  constituir la primera materia del plan de estudio de la carrera, permite la  integración académica pues, posibilita articular el nivel educativo previo y  las exigencias requeridas en el nivel superior.    La muestra está conformada por un 10% del total de estudiantes que  ingresaron a la carrera de Psicología en el periodo lectivo 2012, y la  recolección&nbsp; de información se realiza  mediante la aplicación de un cuestionario que incluye preguntas abiertas y  cerradas, de las cuales para esta oportunidad se ha considerado la pregunta  referida a ¿Qué les posibilitó el Ciclo de Iniciación?    Los resultados &nbsp;proporcionan &nbsp;una completa información sobre el espacio de  ingreso como contexto de formación y socialización.     &nbsp;  ","palabras_clave":"INTEGRACIÓN|COORDINADOR|INGRESANTE|UNIVERSIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1390,"idioma_id":4,"titulo":"Helical cell wall thickenings in root cortical cells of Polypodiaceae species from northwestern Argentina","resumen":"The occurrence of helical cell wall thickenings in fern roots is not well investigated and there are few records about it in the literature. To assess the presence of thickenings and their chemical composition, we studied all species of Polypodiaceae, which grow in northwestern Argentina, using light microscopy, scanning electron microscopy, and fluorescence microscopy. Twenty of the twenty-one species studied showed the thickening in the roots. Only in Melpomene peruviana are helical cell wall thickenings absent. All thickenings have cellulose as the main compound. The structure of the thickening may be classified as simple, furcate, or anastomosing. All data presented in this paper corroborate the same structure and chemical composition of thickenings previously reported for Aspleniaceae.","palabras_clave":"ANATOMY|EUPOLYPODS I|FERNS|EPIPHYTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1391,"idioma_id":4,"titulo":"HeteroGenius: A Framework for Hybrid Analysis of Heterogeneous Software Specifications","resumen":"Nowadays, software artifacts are ubiquitous in our lives being an essential part of home appliances, cars, cell phones, and even in more critical activities like aeronautics and health sciences. In this context software failures may produce enormous losses, either economical or, in the worst case, in human lives. Software analysis is an area in software engineering concerned with the application of diverse techniques in order to prove the absence of errors in software pieces. In many cases different analysis techniques are applied by following specific methodological combinations that ensure better results. These interactions between tools are usually carried out at the user level and it is not supported by the tools. In this work we present HeteroGenius, a framework conceived to develop tools that allow users to perform hybrid analysis of heterogeneous software specifications. HeteroGenius was designed prioritising the possibility of adding new specification languages and analysis tools and enabling a synergic relation of the techniques under a graphical interface satisfying several well-known usability enhancement criteria. As a case-study we implemented the functionality of Dynamite on top of HeteroGenius.","palabras_clave":"Theorem proving|Heterogeneous Specifications |Software Analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1392,"idioma_id":5,"titulo":"LOS MARCOS DE REFERENCIA EN EL POSICIONAMIENTO SATELITAL A  TIEMPO REAL","resumen":"Real time (RT) satellite positioning services offered by the latinamerican countries are using the Internet Protocol NTRIP (Networked Transport of RTCM via Internet Protocol) for transmitting differential corrections from GNSS permanent stations (Servers-NTRIP). Users can be positioned with precision at the moment of measurement and get the coordinates in the reference frame implicit in the base station coordinates from which the corrections was received. Therefore, a correct configuration of RT services, will ensure users not only to refer to the appropriate reference frame but also obtain optimal precision in the resulting coordinates.  Then the objective of this work was to analyze the current situation of the services offered in Brazil, Uruguay and Argentina. The configuration of the reference stations as well as the regional caster has been studied to accomplish such object. Each RTCM messages transmitted from server stations was also analyzed in detail. The  184  reference frames of the coordinates were verified, as well as the reference to it in the publishing site. It was found that a user does not have information of the framework at the time of measurement.  Results of experiences performed in the Uruguay - Argentina border zone were presented. Experimental streams was created from the same station on three different reference frames: POSGAR 2007 (Arg) , ROU 98 (Uru.) and weekly SIRGAS coordinates. These results quantify the influence of using different reference frames in the accuracy of the real time positioning.  Key words: GNSS, Real Time, NTRIP, Reference Frame, Epoch.","palabras_clave":"NTRIP|RTK|Posicionamiento a Tiempo real|GPS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1393,"idioma_id":5,"titulo":"Comportamiento ingestivo y respuesta productiva de novillos en pasturas de Lotus tenuis","resumen":"El objetivo de este trabajo fue evaluar la respuesta animal en términos de comportamiento ingestivo, consumo y ganancia de peso de novillos británicos durante la etapa de terminación sobre una pastura de lotus (Lotus tenuis) ofrecida en dos horarios del día, durante el período estival. El ensayo se realizó en el Campo Experimental de INTA Chascomús, Buenos Aires,Argentina (35º 56? S, 57º 83? W). Se estudiaron dos tratamientos: T1 (pastoreo matutino) y T2 (pastoreo vespertino) en tres períodos: PI (10\/12\/12 al 2\/01\/13), PII (3\/01\/13 al 25\/01\/13) y PIII (26\/01\/13 al 20\/02\/13). Se utilizaron dos grupos de 10 novillos de raza británica (311 ± 3,9 kg). En los 3 últimos días de cada período se midió comportamiento ingestivo (pastoreo, rumia y descanso) de 09:00 a 13:00 y de 13:00 a 17:00, consumo de forraje y ganancia de peso. La calidad del forraje ofrecido fue analizada. Los datos obtenidos se analizaron para cada período en un diseño completo aleatorizado utilizando un modelo lineal. Los resultados indican que en PII el pastoreo fue mayor por la tarde (p<0,05) e igual tendencia se observó en PIII (p=0,07). Resultados similares se obtuvieron para la rumia. Los animales pastoreando por la tarde evidenciaron una tendencia superior para el consumo de forraje en los tres períodos (1066 vs. 1288 Kg MS ha-1), con diferencias significativas solo en PIII (p<0,05). En el grupo de pastoreo vespertino, las ganancias de peso tendieron a ser superiores, aunque solo significativamente mayores en PIII (p<0,05). Se concluye que el horario de pastoreo produjo cambios en el comportamiento ingestivo de los animales, observándose que los pastoreos vespertinos de Lotus, pueden favorecer el consumo y consecuentemente el proceso de engorde.Palabras clave: comportamiento ingestivo, lotus, consumo.","palabras_clave":"COMPORTAMIENTO INGESTIVO|LOTUS|CONSUMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1394,"idioma_id":5,"titulo":"Acerca del programa de la organización Montoneros: ¿reformistas o revolucionarios?","resumen":"Las organizaciones políticas son caracterizadas a partir de su accionar militante. De esta manera, su carácter reformista o revolucionario se mide a partir del desarrollo de una estrategia más o menos radicalizada. En este sentido, una agrupación que despliega acciones armadas tendría un contenido político más radicalizado que una que no lo hace. Sin embargo, a partir del análisis del programa de Montoneros podremos observar que el despliegue de acciones armadas no asegura la defensa de un programa revolucionario. Por lo tanto, para el conocimiento de la naturaleza de una organización política es necesario abordarla desde su análisis programático.","palabras_clave":"MONTONEROS|PROGRAMA|REVOLUCIÓN|REFORMISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1395,"idioma_id":5,"titulo":"Montoneros a la luz de sus documentos","resumen":"En este artículo reconstruimos los puntos programáticos de Montoneros a partir de ocho ejes problemáticos que aparecen a lo largo de sus documentos: sus antecedentes históricos, la historia argentina, la política mundial, la economía argentina y las contradicciones de clase, el Estado, los objetivos, el sujeto y sus aliados, qué es el peronismo y el debate en torno a la fusión con las Fuerzas Armadas Revolucionarias (FAR). A su vez, estos núcleos programáticos están atravesados por dos etapas cronológicas: 1970-1971 y 1972-1976. La primer, vinculada al proceso de insurrección de masas y la segunda etapa, como parte del proceso iniciado con el Gran Acuerdo Nacional (GAN). Esta reconstrucción tiene como objetivo responder a la pregunta de cuál fue el rol que desarrolló Montoneros dentro del proceso revolucionario en el período 1969-1970. Sostenemos como hipótesis que su función fue la de contener el potencial desarrollo de la fuerza social revolucionaria que se formó en esa instancia. Para realizar este trabajo analizamos los documentos públicos e internos de la organización durante el período señalado, así como también entrevistas a sus principales dirigentes.","palabras_clave":"MONTONEROS|PROGRAMA|LIBERACION NACIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1398,"idioma_id":5,"titulo":"Afroargentinos, guerra y política, durante las primeras décadas del siglo XIX. Una aproximación hacia una historia social de la revolución","resumen":"este artículo es&nbsp;una primera exploración sobre la participación de los afroargentinos en las Guerras de la Independencia durante las primeras décadas del siglo XIX. Me detengo de manera particular en el análisis historiográfico sobre el tema (Rodríguez Molas, Andrews, Goldberg, Mallo,&nbsp;Crespi, Bragoni, Mata) y en los avances que se dieron en los últimos años en torno a los festejos del Bicentenario de la revolución.&nbsp;&nbsp;Asimismo en las contribuciones provenientes de la historia social, política y cultural rioplatense (Sábato, Fradkin, Rabinovich, Di Meglio, Gelman). Por último, hago hincapié &nbsp;en las nuevas preguntas y áreas de vacancias relacionadas con el papel que les cabe a los afroargentinos en la historia de las revoluciones argentinas y en la retórica de la 'desaparición' como 'carne de cañón', que todavía constituye uno de los mitos de mayor recurrencia en la sociedad argentina.                                 ","palabras_clave":"GUERRA|POLITICA|AFRODESCENDIENTES|REPRESENTACIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1400,"idioma_id":4,"titulo":"Efficiency in Brazil's water and sanitation sector and its relationship with regional provision, property and the independence of operators","resumen":"The purpose of this paper is to assess the comparative efficiency of Brazil´s water and sanitation sector. We run a Stochastic Frontier Analysis (SFA) model for a panel of 127 providers covering more than 70 percent of the country´s urban population in the period 2003?2010. We use a database built on the National System of Sanitation Statistics (SNIS). The model is fitting and shows a modest efficiency average. The study has policy implications in the discussion of state-level run v. municipal-level run, government-owned v. private-provision, and corporatized providers v. dependent ones. The optimal industrial organization of the sector is discussed from the efficiency perspective. We find that regional and micro-regional firms´ have lower costs than municipal providers. Administrative independence seems not relevant when explaining the cost structure, but ownership is. The joint provision of water and sanitation results in higher fixed costs compared to water-only operators. We find that there are not regional differences in cost structures. However, there are slight variations in the efficiency levels and in their dispersion in each region. Finally, inefficiency decreased at a rate of 4.9 percent per year during the time frame under study.","palabras_clave":"Efficiency|Water sector|Brazil|Public-private provision"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1401,"idioma_id":5,"titulo":".  Autopodios de guanacos en sitios arqueológicos: equifinalidad  entre lo palatable y lo preservado","resumen":"Abstract  The frequency of  autopodial remains of guanacos (Lama guanicoe) in Patagonia archaeological sites indicates that these units present a finding oversized against other anatomical parts of the skeleton. The usual responses to these patterns are related to taphonomic issues or differential butchering and transport. Therefore, these units with low economic return and high survival rate, present an equifinality problem. These hypotheses are discussed in light of the comparative analysis of sets zooarchaeological Central Plateau of Santa Cruz, which, combined with ethnographic and chemical composition of fats of artiodactyls extend zooarchaeological interpretations helping to minimize equifinality.    Key words: hunter-gatherers, zooarchaeology, Patagonia, Mid Holocene.","palabras_clave":"zooarqueología|Holoceno|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1402,"idioma_id":5,"titulo":"Arte rupestre en la meseta de somuncurá: grabados y pinturas a escala regional","resumen":"En este trabajo se presenta el análisis comparativo de tres localidades con arte rupestre asociadas a lagunas en el sector oriental de la meseta de Somuncurá: Laguna La Maciega, Laguna Azul y El Ganso. Esta información se inserta, posteriormente, en un contexto regional, considerando una amplia diversidad de sitios con manifestaciones rupestres ubicados sobre esta altiplanicie y en sus inmediaciones. Los resultados obtenidos en una escala mesorregional señalan una alta diversidad de motivos y técnicas que se acentúa en Laguna La Maciega. Esta diversidad, junto con el análisis de las pátinas, sugiere la posibilidad de usos recurrentes de las mencionadas cuencas. La evaluación de los contextos arqueológicos asociados al arte rupestre en Nordpatagonia permite enmarcar a la circulación de estas imágenes en la dinámica social del Holoceno tardío.    Palabras clave: Nordpatagonia, cazadores recolectores, Holoceno tardío, petroglifos, pictografías.    Abstract  This paper analyses three localities with rock art associated to lagoons in the eastern portion of the Somuncurá Plateau: Laguna La Maciega, Laguna Azul and El Ganso. This information is framed in a regional context, considering a wide diversity of sites with rock art located over and near the plateau. The results obtained in a mesoregional scale show a large diversity of motifs and techniques, which is accentuated in Laguna La Maciega. This diversity, together with the analysis of the patina, suggests the possibility of recurrent uses of these basins. According to the evaluation of the archaeological contexts associated to the rock art in North Patagonia, it is most likely that the circulation of these images corresponds to a late Holocene social dynamic.    Key words: North Patagonia, hunter gatherers, late Holocene, petroglyphs, pictographs.","palabras_clave":"arte rupestre|Patagonia|Holocno|cazadores-recolectores"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1403,"idioma_id":5,"titulo":"Conflictos, solidaridades y miradas en torno a la situación de calle","resumen":"Revista indexada en Catálogo de Latindex, IBSS, Iresie (Indice de Revistas de Educación Superior e Investigación Educativa),Red ALyC, Social Planning\/Policy & Development Abstracts, Sociological Abstracts, Ulrich´s.      La experiencia de vivir en la calle generalmente suele asociarse a quienes pernoctan en la vía pública dejando de lado a otros actores que también están involucrados en torno a ésta: la policía, las patotas nocturnas, los vecinos, etcétera. El objetivo de este artículo es caracterizar las relaciones que existen entre los diferentes actores que forman parte, directa o indirectamente, de la situación de calle a partir de los testimonios de los adultos que viven en la calle (AVC) recogidos durante el trabajo de campo que culminó con la escritura de la tesis de doctorado. En el entramado de relaciones que se teje en el marco del vivir en la calle se hace presente el Gran Otro (el resto de la sociedad que no duerme en la calle) con quien los AVC mantienen cercanías y lejanías; solidaridades y conflictos.","palabras_clave":"CONFLICTO SOCIAL|SOLIDARIDAD|VIVIR EN LA CALLE|ESPACIO PÚBLICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1404,"idioma_id":4,"titulo":"Testicular function during adolescence in boys with type 1 diabetes mellitus (T1D): absence of hypogonadism and differences in endocrine profile at the beginning and end of puberty.","resumen":"Abstract  AIM:  Conflicting results regarding testicular function in adults with type 1 diabetes (T1D) have been reported, but little is known about Leydig and Sertoli cell function during puberty in boys treated with multiple daily insulin doses. Our aim was to assess testicular function in boys with T1D.  METHODS:  Pubertal boys with T1D (n&#8201;=&#8201;71) and healthy control boys (Control group; n&#8201;=&#8201;104) who were 10-18&#8201;years were studied. Both groups were matched by pubertal stage, age, and BMI. Total testosterone (TT), calculated free testosterone (cfT), SHBG, inhibin B, AMH, and gonadotropin levels were determined.  RESULTS:  At the beginning of puberty, the T1D group had higher levels of SHBG (p&#8201;=&#8201;0.003) and similar androgen levels than the Control group. At the end of puberty, higher TT, and cfT were observed in T1D compared to the Control group (p&#8201;<&#8201;0.01 and p&#8201;<&#8201;0.001, respectively). Gonadotropins and AMH were similar in both groups. Regression analysis showed that T1D was a significant factor, even after adjusting for Tanner stage and BMI-SDS, affecting TT, cFT, and SHBG levels. BMI-SDS was a significant factor affecting TT and SHBG levels. Higher HbA1c had a negative effect on total testosterone and cFT and a positive effect on SHBG levels in T1D boys.  CONCLUSION:  Adolescents with T1D do not exhibit hypogonadism, as shown by normal gonadotropin, testosterone, inhibin B, and AMH levels. However, in T1D boys, HbA1c and BMI-SDS had a negative association with testosterone levels. Elevated testosterone levels are observed during late puberty, which were not present earlier.","palabras_clave":"Complication|Diabetes mellitus type 1|Puberty| Testosterone"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1406,"idioma_id":4,"titulo":"Media, children and play: New practices in a new (and complex) ecosystem","resumen":"The relationship between media and play has been the subject of my research during the last ten years. Along that period, the research has obtained a common conclusion: children are related to media as consumers, as audiences, as producers and as reproducers of what is shown on them. From the moment in which children are born a new ecosystem composed by different screens and devices becomes their home. I argue that play is a key dimension to analyze contemporary childhoods and their relationship to media culture. When children talk about their games, their favorite shows and computer interactions, they are presenting themselves to others. The objective of this paper is double. Firstly, I aim to present a characterization of the media addressed specifically to children in order to identify continuities, stereotypes, differences and basic characteristics. The key concept in this phase is the one of representation. Secondly, I work with the interviews that I made during 2012 to thirty children who were between six and ten, inhabitants of the City of Buenos Aires, Argentina. The objective was to listen to children and the meanings the built around their everyday lives. Play and games were approached not only as meaningful cultural practices but also as a way of analyzing the productions that children made from the available discourses in their everyday lives. Desires, preferences and games as children name them are going to be the organizers of the second part of the paper. The relationship between children, media and play is complex, but it is a very interesting way to build some knowledge about the contemporary culture and the ways through which children establish different relationships with it. Both objectives will allow us to build a complex approach to children, media and play in the communicative ecosystem in which they are growing.","palabras_clave":"Play|Media|Children|Ecosystem"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1408,"idioma_id":5,"titulo":"Explotación de peces y guanacos en el interior de Patagonia central: aportes del sitio Delta del Arroyo Vulcana 1 (lago Musters, Chubut)","resumen":"La subsistencia de los cazadores recolectores de Patagonia central estuvo orientada a la explotación de guanaco (Lama guanicoe); sin embargo, en los ambientes fluviolacustres existen potenciales recursos dulceacuícolas para explotar. Esto ocurre en la cuenca del lago Musters donde se ha evidenciado la explotación de peces fluviales en dos sitios del Holoceno tardío: Laguna de la Flecha 10 y Delta del Arroyo Vulcana 1 (DV1). En este trabajo presentamos los resultados finales del análisis faunístico de DV1; sitio que funcionó como campamento base y que se caracteriza por una marcada estructuración espacial. Los resultados obtenidos permiten establecer al agente antrópico como el principal acumulador del conjunto, el cual está compuesto principalmente por elementos óseos de guanaco y perca (Percichthys trucha). Los indicadores estacionales señalan que la ocupación de DV1 ocurrió durante la primavera y el verano. En la primavera, época reproductiva de la perca, se producen grandes agregaciones de peces en las aguas someras, lo cual propicia condiciones favorables de disponibilidad y captura. Se concluye que el guanaco fue el recurso de mayor importancia en la subsistencia de este grupo y su explotación se orientó hacia la extracción de grasa ósea. Los peces tuvieron un rol complementario y aportaron grasa y nutrientes esenciales a la dieta.                           ","palabras_clave":"SUBSISTENCIA|CAZADORES-RECOLECTORES|LAGO MUSTERS|PATAGONIA CENTRAL|HOLOCENO TARDÍO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1409,"idioma_id":4,"titulo":"Photodegradation of chlorpyrifos in water by UV\/H2O2 treatment. Toxicity evaluation","resumen":"Chlorpyrifos (CP) is an organophosphorous pesticide widely used in agriculture. Due to its toxicity, it has been necessary to develop a treatment to eliminate the environmental wastes generated by this substance. The combination of UV radiation and hydrogen peroxide seemed an interesting option to reduce the concentration of CP in water, as it has proved to be a simple and effective method to degrade other similar pesticides. The purpose of this work is to study the effect of different initial concentrations of H2O2 on the degradation of commercial chlorpyrifos in water using UV\/H2O2. Conversions of 93% were achieved in 20 min with 450 mgL-1 of H2O2 initial concentration (0.16 mg of CP degraded per mg of H2O2 consumed). The total organic carbon (TOC) analysis showed interesting mineralization values, reaching a 70% conversion after 4 h of reaction. In addition, this degradation procedure leads to a clear reduction of the toxicity of the reaction mixture over Vibrio fischeri.","palabras_clave":"chlorpyrifos|UV\/H2O2|toxicity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1410,"idioma_id":4,"titulo":"Press and social networking services in the Internet: Approaches to the relation between two Argentine online newspapers with Facebook and Twitter","resumen":"This article describes the incorporation of the social networks resources on Internet into digital interfaces of the two main Argentine newspapers, Clarín and La Nación, as well as the ways in which these online newspapers manage and use their official accounts on Facebook (FB) and Twitter (TW). Such reflections are part of the first stage of a research project which aims to understand the relationship established, at present, between digital media and social networking, to account for the impact of the latter both the link to the press with his readership as in the press-public sphere relationship. As we have seen until now, these observed online newspapers use their official accounts on FB and TW for several purposes: to get viral spread of content; to establish a direct contact with the public; to get the audience involved and encourage  their participation; to consolidate their own positions into the social networks; and to get users to control the quality of his speeches on the Internet. Besides, the incorporation of the social networks resources on these online newspapers would impact, specially, on news circulation process since a piece of news is no longer a stable unit and gets modified as the story travels through Internet social networks.","palabras_clave":"PRENSA ONLINE|REDES SOCIALES EN INTERNET|FACEBOOK|TWITTER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1411,"idioma_id":4,"titulo":"Nuclear actin polymerization from faster growing ends in the initial activation of Hox gene transcription are nuclear speckles involved?","resumen":"The HoxB cluster  expression is activated by retinoic acid and transcribed in a collinear manner.  The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here,  NT2-D1 teratocarcinoma cells, a model of Hox  gene expression, were used to show that upon retinoic acid induction, Pknox1  co-localizes with polymeric nuclear actin. We have found that globular actin  aggregates, polymeric actin, the elongating RNA polymerase II and THOC match  euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase  II, N-WASP, and transcription\/splicing factors p54nrb and PSF were  validated as Pknox1 interactors by tandem affinity purification. PSF pulled  down with THOC and nuclear actin, both of which co-localize in nuclear  speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of  nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner.  Thus, our results support the hypothesis that nuclear actin polymerization is  involved in the activation of HoxB  gene expression by means of nuclear speckles.              ","palabras_clave":"Nuclear Actin|HOX genes|transcriptional regulation|RNA PolII"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1413,"idioma_id":5,"titulo":"Las 'fábulas trágicas' que corrompen la sociedad. Condena moral de la literatura popular en la prensa argentina a principios del siglo XX","resumen":"A comienzos del siglo XX en la Argentina, la atención particular de la prensa a los folletines produce un discurso de rechazo a una retórica popular por lectores asociados a una intelectualidad ilustrada, comprometida en la construcción de un sentimiento universalista moralizante a través de la alta cultura.  Este es el caso concreto del artículo Las fábulas trágicas, de Federico Quevedo Hijosa,  publicado por el vespertino tucumano El Orden (01\/03\/1912). En el presente trabajo, analizamos su argumentación a fin de develar los presupuestos que intentan despojar la literatura de los elementos populares considerados inmorales.","palabras_clave":"FOLLETINES|TUCUMAN|LITERATURA POPULAR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1414,"idioma_id":5,"titulo":"¿Cómo pensar el afecto en la política? Aproximaciones y debates en torno a la teoría de la hegemonía de Ernesto Laclau","resumen":"Este artículo se propone aprehender la forma en la que la dimensión del afecto es problematizada en la teoría de la hegemonía de Laclau, a partir de una reconstrucción de sus principales conceptos teóricos así como de los debates intelectuales que se han suscitado al respecto. Presentaremos un recorrido transversal por las producciones del autor a fin de reconstruir sucintamente los elementos fundamentales que componen su teoría. Posteriormente, profundizaremos sobre la incorporación de la dimensión afectiva como un componente primario de todo proceso de construcción hegemónica, explicitando la imposibilidad de reducir esta última a operaciones estrictamente lingüísticas. Abordar el vínculo entre hegemonía y economía afectiva permite revalorizar el aporte teórico de Laclau al identificar las herramientas heurísticas que este brinda para el análisis sociopolítico.","palabras_clave":"LACLAU|HEGEMONIA|AFECTO|POLITICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1415,"idioma_id":4,"titulo":"Construction of Specific Parallel Amplification of RNA Ends (SPARE) libraries for the systematic identification of plant microRNA processing intermediates","resumen":"MicroRNAs (miRNAs) are small RNAs that derive from endogenous precursors harboring foldback struc- tures. Plant miRNA precursors are quite variable in their size and shape. Still, the miRNA processing machinery, consisting of DICER-LIKE1 (DCL1) and accessory proteins recognize structural features on the precursors to cleave them at specific places releasing the mature miRNAs. The identification of miRNA processing intermediates in plants has mostly relied on a modified 50 RACE method, designed to detect the 50 end of uncapped RNAs. However, this method is time consuming and is, therefore, only practical for the analysis of a handful miRNAs. Here, we present a modification of this approach in order to perform genome-wide analysis of miRNA processing intermediates. Briefly, a reverse transcription is performed with a mixture of specific primers designed against all known miRNA precursors. miRNA processing intermediates are then specifically amplified to generate a library and subjected to deep sequencing. This method, called SPARE (Specific Parallel Amplification of 50 RNA Ends) allows the identification of process- ing intermediates for most of the Arabidopsis miRNAs. The results enable the determination of the DCL1 processing direction and the cleavage sites introduced by miRNA processing machinery in the precursors. The SPARE method can be easily adapted to detect miRNA-processing intermediates in other systems.","palabras_clave":"microRNA|Precursor|Processing|Arabidopsis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1416,"idioma_id":5,"titulo":"Aportes desde el legado de la filosofía latinoamericana.  Intervenciones desde los intersticios: sujeto, historia, cultura.","resumen":"Aportes desde el legado de la filosofía latinoamericana.  Intervenciones desde los intersticios: sujeto, historia, cultura.    Dra. Paola Gramaglia  (CIFFyH-UNC)  Dra. María Cristina Liendo  (CIFFyH-UNC)  Lic. Ana Britos Castro  (CONICET-CIFFyH-UNC)  Lic. Manuel Fontenla  (CIFFyH-UNC)        «El propósito de la teorización no es para hacerse una reputación académica o intelectual, sino para permitirnos asir, entender y explicar -para producir un más adecuado conocimiento de- el mundo histórico y sus procesos, y de ese modo configurar nuestra práctica y así poder transformarlo»    Stuart Hall           En este trabajo que es fruto de largas conversaciones, debates, presentaciones a congresos y jornadas nos interesa poner en consideración los ejes teóricos principales que hemos estado trabajando desde hace un tiempo algunos integrantes del Grupo de investigación de Filosofía latinoamericana de la Facultad de Filosofía y Humanidades (UNC).  En primer lugar,  dejar en claro que partimos de la afirmación de la especificidad, de nuestro saber ?la filosofía latinoamericana -, en el contexto académico-disciplinar en el que nos encontramos: la Escuela de Filosofía y dentro de la cual llevamos adelante nuestras tareas e investigaciones. Remarcamos nuestra posición, porque nos interesa atender qué sea la filosofía latinoamericana: sus tradiciones, programas de estudio, líneas de investigación, políticas de circulación y producción científica, etc. Posición que consideramos parte de un proyecto crítico de la episteme  moderna, y que por tanto no podría realizarse sólo desde la reivindicación de un determinado localismo, ni desde una determinada crítica a la ?universalidad? de su racionalidad.  En esta dirección nos apropiamos del legado de la filosofía latinoamericana como historia de las ideas, tal como la sustentan Arturo Roig y Horacio Cerutti Guldberg para desde allí, con nuevas herramientas, conceptos, categorías y definiciones, enriquecer y complejizar nuestras prácticas discursivas para una teoría crítica latinoamericana. Teniendo cuidado, cómo nos advierte Rancière que, ?Lo que ha tenido lugar no es el fin de la gran narrativa de la Modernidad. Es el reciclado y readaptación de los componentes de esa narrativa en un intento activo de configurar un orden de dominación capaz de desterrar cualquier resistencia y excluir cualquier alternativa imponiéndose a sí mismo como manifiesto e ineludible.?","palabras_clave":"La cuestión del discurso|cultura|subalternidad|sujetividad política"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1417,"idioma_id":4,"titulo":"Summary-based inference of quantitative bounds of live heap objects","resumen":"This article presents a symbolic static analysis for computing parametric upper bounds of the number of simultaneously live objects of sequential Java-like programs. Inferring the peak amount of irreclaimable objects is the cornerstone for analyzing potential heap-memory consumption of stand-alone applications or libraries. The analysis builds method-level summaries quantifying the peak number of live objects and the number of escaping objects. Summaries are built by resorting to summaries of their callees. The usability, scalability and precision of the technique is validated by successfully predicting the object heap usage of a medium-size, real-life application which is significantly larger than other previously reported case-studies.","palabras_clave":"Resource consumption|Quantitative analysis|Heap memory requirements"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1418,"idioma_id":4,"titulo":"The low-abundance transcriptome reveals novel biomarkers,","resumen":"  Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of  tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and  targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome.  The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis  using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the  low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the  identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified  cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNc, VEGF-C, WISP1,  FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking  down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that  the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of  cancer.","palabras_clave":"gastric cancer|transcriptome|biomarker"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1419,"idioma_id":5,"titulo":"LOS CENTROS CÍVICOS 'MARÍA EVA DUARTE DE PERÓN': PUNTALES DE UN LIDERAZGO Y DE REACOMODAMIENTOS PARTIDARIOS (1947)","resumen":"Hacia fines de 1947, el escenario político dejo abierta una nueva dimensión del peronismo, la trilogía de la revolución: Perón, Eva, Mercante. Este trabajo analiza ese momento de armado y articulación política del liderazgo de Eva Perón a través de los centros cívicos femeninos, la rama femenina del novel Partido Peronista, las primeras damas provinciales y la ley de sufragio femenino, entramado de una nueva linea interna del peronismo.http:\/\/www.latindex.unam.mx\/buscador\/ficRev.html?opcion=1&folio=2981","palabras_clave":"Eva Peron |Centros civicos femeninos Maria Eva Duarte de Perón|Rama femenina del Partido Peronista|Sufragio femenino|Mujeres peronistas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1420,"idioma_id":4,"titulo":"Spatial and temporal analysis of infant mortality from congenital malformations in Brazil (1996-2010).","resumen":"While there are a number of studies on infant mortality in general and its risk factors, the contribution of congenital malformations and the mortality rates of children due to them has been less explored. Although individually rare, congenital malformations taken together have contributed significantly to infant mortality in populations where infectious diseases have been controlled and nutritional deficiencies have been corrected (Jenkins 1977).    Over the last decades, in countries at different stages of development, there has been a trend of decrease in total infant mortality accompanied by a reduction in the rate of infant mortality from congenital malformations (IMR-CM), that is, the number of deaths from malformations per 1,000 births. The proportion of congenital malformation deaths (CMD%) has also been increasing, that is, the number of deaths from malformations per 100 deaths, for children less than 1 year of age (Rosano et al. 2000; Copeland and Russell 2006). The previously described trend of deaths in children under 1 year of age was confirmed in Chile in the period from 1985 to 2001 (Szot 2004) and in Argentina in the period from 2002 to 2006 (Bronberg et al. 2009). According to Rosano et al. (2000), the IMR-CM tends to be higher in the poorest countries, while the CMD% is higher in richer ones.    In most Latin American countries, congenital malformations as the cause of death for children less than 1 year of age has tended to increase, and it has occupied between second and fifth places as the cause of death, contributing between 2 % and 27 % to total infant mortality. According to Victora et al. (2011), in Brazil over the last three decades, there has been a persistent and substantial decrease in infant mortality?it decreased 5.5 % a year between 1980 and 1990, and it has decreased 4.4 % a year since 2000, attaining a rate of 20 deaths per 1,000 live births in 2008. According to these same authors, in the period between 1990 and 2007, there was no significant change in the IMR-CM throughout Brazil. Similar findings were reported by Victora and Celso (2001) when analyzing the behavior of infant mortality due to congenital malformations in Brazil between 1985 and 1987 and 1995 and 1997, thus demonstrating that, while the IMR-CM remained stable in both periods, the CMD% increased from 7.1 % for 1985?1987 to 11.5 % for 1995?1997. The same trend occurred at the regional level but with disparities between regions, particularly for the CMD%. However, Siedersberger Neto et al. (2012) found an increase in both the IMR-CM and the CMD% throughout Brazil in the period between 1996 and 2008.    This paper intends to broaden the analysis of the IMR-CM and CMD% trends in Brazil for 1996?2010 as well as their spatial variation at different levels of political?administrative structures (regions, Federative Units\/States, and municipalities).","palabras_clave":"infant mortality|congenital malformations|genetic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1421,"idioma_id":5,"titulo":". Experimentación y propiedades combustible de especies del bosque Andino-Patagónico. Aportación al estudio antracológico de sitios arqueológico","resumen":"The properties of woody fuels during the combustion can complement the interpretation of the modes of acquisition and use of firewood by groups of the past. The experimental method allows to deepen about the properties of different plant species as fuel, but more importantly allows the reconstruction of the processes that have generated the archaeobotanical remains recovered. This paper has two objectives. For one determine the combustion properties of four woody species (Austrocedrus, Fitzroya cupressoides, Nothofagus and Nothofagus antarctica dombeyi) and understand their behavior in the combustion process. Furthermore, explore whether shrinkage cracks present in the charcoal respond to water content the firewood (green-dry). The performance of 16 experimental combustions and the study of the charcoal produced during combustion show differences between woody species studied. The results allow, in some cases, to discriminate the use of dry wood and green wood as fuel. The results obtained allow on one hand, discriminate the use of dry wood and green wood as fuel. On the other, deepen the identification and interpretation of the alterations present in the archaeological charcoals and complement the archaeobotanical analysis of the different sites Patagonia.","palabras_clave":"EXPERIMENTACIÓN|PROPIEDADES COMBUSTIBLES|BOSQUE ANDINO-PATAGÓNICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1422,"idioma_id":4,"titulo":"On the correspondence between tree representations of chordal and dually chordal graphs","resumen":"Chordal graphs and their clique graphs, dually chordal graphs, possess characteristic tree representations, namely, clique trees and compatible trees, respectively. The following problem is studied: given a chordal graph $G$, it has to be determined if the clique trees of $G$ are exactly the compatible trees of its clique graph. This leads to a new subclass of chordal graphs, the basic chordal graphs, which are here characterized. The question is also approached backwards: given a dually chordal graph $G$, we find all the chordal graphs of which $G$ is clique graph such that the correspondence between clique trees and compatible trees is satisfied. This leads to the possibility of considering several properties of clique trees of chordal graphs and finding their counterparts in compatible trees of dually chordal graphs.","palabras_clave":"CHORDAL GRAPH|DUALLY CHORDAL GRAPH|BASIC CHORDAL GRAPH|CLIQUE TREE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1423,"idioma_id":4,"titulo":"Ocular And Craniofacial Phenotypes In A Large Brazilian Family With Congenital Aniridia.","resumen":"Congenital aniridia is a rare genetic disorder characterized by varying degrees of iris hypoplasia that are associated with additional ocular abnormalities. More than 90 % of the causal mutations identified are found in the PAX6 gene, a transcription factor of critical importance in the process of neurogenesis and ocular development. Here, we investigate clinical, molecular, and craniofacial features of a large Brazilian family with congenital aniridia. Among the 56 eyes evaluated, phenotype variation encompassed bilateral total aniridia to mild iris defects with extensive variation between eyes of the same individual. PAX6 molecular screening indicated a heterozygous splice mutation. Thus, we hypothesize that this splicing event may cause variation in the expression of the wild-type transcript, which may lead to the observed variation in phenotype. Affected individuals were more brachycephalic, even though their face height and cephalic circumference were not significantly different when compared to those of non affected relatives. From this, we infer that the head shape of affected subjects may also be a result of the PAX6 splice site mutation. Our data summarize the clinical variability associated with the ocular phenotype in a large family with aniridia, and help shed light on the role of PAX6 in neurocranial development.","palabras_clave":"aniridia|autosomal dominant|neurocranium|PAX6"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1424,"idioma_id":4,"titulo":"A new hadrosaurid mandible from the latest Maastrichtian of the Pyrenees (north Spain) and the high diversity of the duck-billed dinosaurs of the Ibero-Armorican Realm at the very end of the Cretaceous","resumen":"In the latest Maastrichtian, the European hadrosauroid fauna was more diverse than those of North America and Asia. The European record of hadrosaurid dentaries is an example of this diversity, and most of the sites with mandibular remains are located in the Ibero-Armorican Realm. Within the Iberian Peninsula, most of the remains are located in the Tremp Basin (South Central Pyrenees). Two of the three valid hadrosaurid taxa defined in this basin are from the Blasi sites (Arén, Huesca province): Arenysaurus ardevoli (Blasi-3) and Blasisaurus canudoi (Blasi-1). A new locality in Blasi (Blasi 3.4) has provided a new dentary from an indeterminate euhadrosaurid. This dentary presents some characters intermediate between Arenysaurus and Blasisaurus, some characters similar to Pararhabdodon isonensis (from the nearby province of Lleida), and some characters of its own. Nevertheless, due to its fragmentary character, without dentition or its edentulous anterior part, it cannot be determined above the level of Euhadrosauria. It thus represents a fourth Iberian euhadrosaurian taxon in the Ibero-Armorican Realm, different from Arenysaurus, Blasisaurus and Pararhabdodon, increasing the diversity of hadrosauroids in this realm at the very end of the Cretaceous.","palabras_clave":"Dentario|Hadrosaurio|Maastrichtiense|España"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1425,"idioma_id":4,"titulo":"Effect of extrusion conditions on physicochemical and sensorial properties of corn-broad beans (Vicia faba) spaghetti type pasta","resumen":"Corn-broad bean spaghetti type pasta was made with a corn\/broad bean flour blend in a 70:30 ratio, through an extrusion-cooking process (Brabender 10 DN single-screw extruder with a 3:1 compression ratio). The effect of temperature (T = 80, 90 and 100 C) and moisture (M = 28%, 31% and 34%) on the extrusion responses (specific consumption of mechanical energy and pressure) and the quality of this pasta-like product (expansion, cooking-related losses, water absorption, firmness and stickiness) was assessed. The structural changes of starch were studied by means of DSC and XRD. The extrusion-cooking process, at M = 28% and T = 100 C, is appropriate to obtain corn-broad bean spaghetti-type pasta with high protein and dietary fibre content and adequate quality. The cooking characteristics and resistance to overcooking depended on the degree of gelatinisation and formation of amylose\u2013lipid complexes.The critical gelatinisation point was 46.55%; beyond that point, the quality of the product declines.","palabras_clave":"Extrusion-cooking|Gluten-free|Broad beans (Vicia faba)|Corn|Pasta-like product"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1428,"idioma_id":5,"titulo":"Salubridad, sustentabilidad ecológica y costo de tecnologías constructivas para la vivienda de interés social.","resumen":"Esta revista está indizada por Latindex, Dialnet y Caicyt - CONICET.  Resumen: El Centro Experimental de la Vivienda Económica  - CEVE - ha desarrollado distintas tecnologías constructivas para la vivienda de interés social,  desde su fundación (hace 45 años).  En las primeras décadas de la existencia de esta institución la inquietud principal fue la obtención de tecnologías de bajo costo, para colaborar en reducir el déficit habitacional de nuestro país. En la última década esa preocupación continúa, pero se ha incorporado también como premisa colaborar en reducir la contaminación del medio ambiente, con construcciones sustentables; por eso se han desarrollado nuevos componentes constructivos elaborados con  residuos plásticos reciclados.  Por otra parte, la salubridad de las tecnologías constructivas desarrolladas en CEVE no había sido analizada y comparada hasta ahora; por esta razón en este trabajo se comparan las mismas desde los puntos de vista de la sustentabilidad ecológica, el costo y la salubridad.","palabras_clave":"CONSTRUCCION|TECNOLOGÍA|VIVIENDA SOCIAL|SUSTENTABILIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1429,"idioma_id":4,"titulo":"An analysis of a regular black hole interior model","resumen":"We analyze the thermodynamical properties of the regular static andspherically symmetric black hole interior model presentedby Mboyne and Kazanas.Equations for the thermodynamical quantities valid for an arbitrary density pro-file are deduced, and from them we show that the model is thermodynamicallyunstable. Evidence is also presented pointing to its dynamical instability. Thegravitational entropy of this solution based on the Weyl curvature conjecture iscalculated, following the recipe given by Rudjord, Gr&#8709;n and Sigbj&#8709;rn, and it isshown to have the expected behavior","palabras_clave":"no disponible"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1430,"idioma_id":4,"titulo":"The CHUVA Project: how does convection vary across Brazil?","resumen":"CHUVA reveals very diverse cloud processes in tropical continental regions  and contributes to improving satellite precipitation estimation, nowcasting,  cloud-resolving models, and the understanding of cloud electrification.","palabras_clave":"precipitacion|experimento de campo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1431,"idioma_id":5,"titulo":"Servir en la Corte de los Reyes Católicos: dominicos en los oficios de tutor de príncipes y embajador (1490-1516)","resumen":"La relación entre dominicos observantes y monarcas castellanos durante el reinado de los Reyes Católicos se articuló en torno a la actividad de patrocinio regio sobre los conventos de la Orden de Predicadores y al servicio de los frailes en la Corte. Algunos de esos religiosos recibieron el encargo de educar a los príncipes, situación que en general les permitió iniciar una ascendente carrera política y eclesiástica. De hecho, varios de ellos destacaron como embajadores al servicio del rey Fernando hasta el final de su reinado. Tal es el caso de fray Tomás de Matienzo y de fray Bernardo de Mesa,activos miembros de aquella agrupación que José Martínez Millán ha denominado ?partido fernandino?. En el presente estudio se analizarán las trayectorias vitales de ambos personajes, ya sea como vehículos de ciertas corrientes de espiritualidad sumamente atractivas entre los miembros de la aristocracia castellana a principios del siglo XVI, así como en su rol específico de agentes de la política exterior sostenida por la ?facción fernandina?. En este último sentido, examinaremos en particular la cuestión de los beneficios que podía crear a los monarcas el reclutamiento de religiosos en su personal diplomático.","palabras_clave":"Reyes Católicos|Facciones cortesanas|Tutores de príncipes|Embajadores"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1432,"idioma_id":5,"titulo":"La militancia en las fábricas. Una aproximación a la experiencia de Montoneros Sabino Navarro","resumen":"REVISTA INDIZADA EN LATINDEX , CLASE.  En las páginas que siguen nos proponemos analizar la experiencia sindical de Montoneros Sabino Navarro (SN) teniendo en cuenta la vital importancia que revestía desde una perspectiva teórica, el rol asignado a la clase obrera en el desarrollo de un proceso revolucionario.  A diferencia de otras organizaciones de la izquierda peronista, la SN abandonó todo intento por disputar superestructuralmente, priorizando desarrollo de políticas hacia la base. En esta línea, nos proponemos abordar las distintas modalidades de inserción desplegadas en el ámbito de la fábrica, intentando visualizar críticamente la teoría y la praxis desplegada por la organización.","palabras_clave":"sindicalismo|clasismo|alternativismo|Montoneros Sabino Navarro"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1434,"idioma_id":4,"titulo":"High catalytic activity at low temperature in oxidative dehydrogenation of propane with Cr-Al pillared clay","resumen":"A montmorillonite was pillared separately with chromium and aluminum polyoxocations (Cr?C and Al?C respectively) and also with an equimolar mixture of both polioxocations (Al?Cr?C). The resulting solids  were characterized by scanning electron microscopy, energy dispersive spectrometry, thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction and nitrogen adsorption at 196 C. The inclusion  of polycations in the interlamellar space led to modifications in the properties of the clay. An eleven fold increase in specific surface area with respect to the calcined starting clay was found for the Al?C,  whereas a twofold increase was observed for the Cr?C; the value for the Al?Cr?C was only slightly higher than that for the latter. Diffractograms of the Cr?C showed the occurrence of a Cr2O3 phase. Catalytic  activity in oxidative dehydrogenation of propane was assayed in the temperature range from 350 C to 450 C using Ar\/C3H8\/O2 (80\/10\/10) as reactant gas mixture. Propylene selectivity was found to be temperature-  dependent, showing a maximum in the studied temperature range for the catalyst containing only aluminum, whereas remaining practically constant for the Cr-containing catalysts. Highly promising propylene yields at low temperature were obtained with the catalyst containing Al and Cr (Al?Cr?C): 9.3% at 350 C and 10.3% at 450 C.","palabras_clave":"Cr-Al pillared clay|propane|catalysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1435,"idioma_id":4,"titulo":"Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains","resumen":"Methoxychlor is an organochlorine pesticide used worldwide against several insect pests, resulting in human exposure. This pesticide mimics endocrine hormone functions, interfering with normal endocrine activity in humans and wildlife. For this reason, it is imperative to develop methods to remove this pesticide from the environment, and though, bioremediation using microorganisms results as an excellent strategy. Five Streptomyces spp. strains previously isolated from organochlorine-polluted sites and capable to grow and remove methoxychlor were combined as different mixed cultures to increase methoxychlor removal. From the 39 consortia tested, one consortium (Streptomyces spp. A6, A12, A14, M7) was selected because of its high pesticide removal and specific dechlorinase activity to be assayed on slurry and soil systems. This consortium showed higher biomass values (8.3 × 106 ± 5.7 × 105 CFU mL-1) and methoxychlor removal (56.2 ± 2.3 %) on enriched slurry than in non-enriched slurry (7.3 × 105 ± 1.2 × 105 CFU mL-1 and 45.6 ± 7.4 % of pesticide removal). In soil systems, Streptomyces consortium showed higher growth (1.0 × 1011 ± 5.0 × 1010 CFU g-1) than in enriched slurry, although differences in methoxychlor removal between both culture conditions were not statistically significant. Therefore, the selected Streptomyces consortium may be suitable for the development of in situ (soil) and ex situ (slurry bioreactor) bioremediation methods because of their potential to remove methoxychlor from different systems","palabras_clave":"METOXYCHLOR|BIOREMEDIATION|STREPTOMYCES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1436,"idioma_id":4,"titulo":"Dryinidae of the Neotropical region (Hymenoptera: Chrysidoidea)","resumen":"An updated revision of Neotropical Dryinidae is presented. Seven subfamilies, 23 genera and 502 species are treated. Descriptions, geographic distribution, known hosts, natural enemies and type material of each species are presented, together with illustrations of the main morphological characters and keys to the subfamilies, genera and species. Complete lists of references concerning the Neotropical Dryinidae and their hosts are given. The following new genus is described: Peckius Olmi & Virla, gen. nov. (Apodryininae). The following new species are described: Anteon xochipalense Olmi & Virla, sp. nov. (Mexico, Guerrero), Deinodryinus levigatus Olmi & Virla, sp. nov. (Peru, Lamabayeque), Deinodryinus xanthonotatus Olmi & Virla, sp. nov. (French Guiana), Dryinus semiruber Olmi & Virla, sp. nov. (Argentina, Corrientes), Dryinus valens Olmi & Virla, sp. nov. (Dominican Republic, Pedernales), Dryinus xanthopus Olmi & Virla, sp. nov. (French Guiana), Megadryinus cacaonis Olmi & Virla, sp. nov. (Brazil, São Paulo), Gonatopus isabelensis Olmi & Virla, sp. nov. (Ecuador, Galapagos Islands), Gonatopus villamilensis Olmi & Virla, sp. nov. (Ecuador, Galapagos Islands), Neodryinus mayanus Olmi & Virla, sp. nov. (Honduras), and Peckius insularis Olmi & Virla, sp. nov. (Ecuador, Galapagos Islands). The following new synonymies are presented: Deinodryinus kawensis Olmi 2011b (April) (=D. caxiuana Coelho, Aguiar & Engel, 2011 (June), syn. nov.); Dryinus gibbosus Olmi, 1984 (=D. multicarinatus Coelho, Aguiar & Engel, 2011, syn. nov.); Dryinus striatus (Fenton, 1927), (=D. sinopensis Olmi, 1984, syn. nov.; = D. cerrensis Olmi, 2004a, syn. nov.); Gonatopus Ljungh, 1810 (=Trichogonatopus Kieffer, 1909, syn. nov.); Eucamptonyx dromedarius (Cameron 1888) (=E. hansoni Olmi, 1991, syn. nov.); Haplogonatopus hernandezae Olmi, 1984 (=H. crucianus Olmi, 1986, syn. nov.). The following new combinations are established: Eucamptonyx dromedarius (Cameron, 1888), comb. nov. (from Gonatopus); Gonatopus goiasensis (Olmi 1991), comb. nov. (from Trichogonatopus); G. hispidus (Olmi 1984), comb. nov. (from Trichogonatopus); G. longinoi (Olmi, 1998d), comb. nov. (from Trichogonatopus); G. marinoae (Virla, 1997), comb. nov. (from Trichogonatopus); G. neotropicus (Olmi, 1984), comb. nov. (from Trichogonatopus); G. raptor (Fenton, 1927), comb. nov. (from Chalcogonatopus); G. rubriceps (Kieffer, 1909), comb. nov. (from Trichogonatopus); G. stellaris (Virla, 1997), comb. nov. (from Trichogonatopus). The male of Crovettia brasiliana Olmi 1984, is described for the first time.","palabras_clave":"taxonomy|revision|parasitoids|Chrysidoidea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1437,"idioma_id":4,"titulo":"PREDICTION WITH ARTIFICIAL NEURAL NETWORK OF THE EFFECT OF THE SIZE OF THE FRUIT, CONCENTRATION AND TEMPERATURA OF SUCROSE SYRUP IN THE DIFFUSIVITY OF THE AGUAYMANTO (Physalis Peruviana L.) DEHYDRATED BY OSMOSIS AND SUPPLEMENTED WITH CONVECTIVE DRYING","resumen":"Se investiga la selección de la arquitectura de una Red Neuronal Artificial (RNA) que permita predecir el efecto del tamaño del fruto, concentración y tem- peratura de jarabe de sacarosa en el coeficiente de difusividad del aguaymanto (Physalis peruviana L.) deshidratado osmoconvectivamente. El desarrollo se centra en el estudio y análisis del proceso de deshidratación osmótica de aguaymanto en jarabe de sacarosa agitado a 140 rpm, concentrado a 50 y 70 ºBrix, temperaturas de 40 y 60 ºC y diámetros de frutos de 1,8 y 2,0 cm. Se utilizó la red feedforward con algoritmo de entrenamiento Backpropagation y ajustes de peso Levenberg-Marquart. Los parámetros se evaluaron según error cuadrado medio (mse) y porcentaje de error. El modelo neuronal en la predicción del coeficiente de difusividad obtuvo un error promedio mínimo de 0,54%, arquitectura con función de transferencia tansig y 53 neuronas en la capa oculta, función de transferencia lineal purelin en la capa de salida y 6 ciclos de entrenamiento.","palabras_clave":"ARTIFICIAL NEURAL NETWORK|NEURONAL MODEL|OSMOCONVECTIVE DEHYDRATION|DIFFUSIVITY COEFFICIENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1438,"idioma_id":4,"titulo":"Estimation of daytime downward longwave radiation under clear and cloudy skies conditions over a sub-humid region","resumen":"Downward longwave radiation (LW&#8595;) is a relevant variable for meteorological and climatic studies. Good estimates of this term are vitally important in correct determining of the net radiation, which, in turn, modulates the magnitude of the terms in the surface energy budget (e.g., evaporation). In remote sensing applications, the determination of daytime LW&#8595;is required for estimation of the net radiation using satellite data. LW&#8595; is not directly measured in weather stations and then  is estimated using models with surface air temperature and humidity as input. In this paper, we identify the best models to estimate daytime downward longwave radiation from meteorological data in the sub-humid Pampean region. Several wellknown models to estimate LW&#8595; under clear and cloudy skies were tested.We use downward radiation components and  meteorological data registered at Tandil (Argentina) from 2006 to 2010 (840 days). In addition, we propose two multiple linear regression models (MLRM-1 and MLRM-2) to estimate LW&#8595; at the surface for all sky  conditions. The new equations show better performance than the others models tested with root mean square errors between 12 and 16 W m&#8722;2, bias close to zero and best agreements with measured data (r2&#8805;0.85).","palabras_clave":"longwave radiation|model|subhumid humid|linear regresion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1439,"idioma_id":4,"titulo":"The Assessment of Multicultural Strength: The Design and Validation of an Openness to the Other Affective Domain Inventory","resumen":"The classification of character strengths and virtues by Peterson and Seligman (2004) includes 24 strengths and 6 virtues. Although the development of this classification was inspired by diverse cultural traditions, no one strength or virtue centrally focused on cultural aspects. Fowers and Davidov (2006) have proposed a new multicultural  strength or virtue termed as openness to the other. We developed the Openness to the Other Affective Domain Inventory (OADI), a new 6-item measurement instrument to assess affective attraction to the other, i.e., fascination with or attraction to culturally diverse others, and affective aversion to the other, i.e., distrust of or disgust with  culturally diverse others. The results showed evidence of acceptable reliability, incremental and convergent validity, validity with an external criterion, and known group validity for the OADI. Moreover, a confirmatory factor analysis yielded an excepted two-factor model that corresponded to the attraction and aversión  dimension","palabras_clave":"MULTICULTURALISM|POSITIVE PSYCHOLOGY|TEST CONSTRUCTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1441,"idioma_id":5,"titulo":"Una experiencia en formación docente que propicia la participación y el desarrollo del pensamiento en los estudiantes.","resumen":"El presente trabajo, tiene como propósito contar con una experiencia en formación docente, que se llevó a cabo con alumnas que cursaron la asignatura Ciencias Naturales y su Didáctica, correspondiente al tercer año de la carrera de Profesorado y Licenciatura en Educación Inicial.Desde el marco teórico del Proyecto de Investigación en el que participamos, Las Prácticas Docentes en la Universidad y la Formación de Profesores en el campo de la Educación Inicial y Especial, se reconoce la necesidad de propiciar prácticas de enseñanza que acrecienten y potencien las capacidades de los estudiantes.El objetivo es describir una experiencia en formación docente, de prácticas de enseñanza que posibiliten una mayor participación de los alumnos y un avance en los procesos del pensamiento. Para ello se utilizan rutinas de pensamiento, como estrategias que orientan las discusiones en las clases, generando una cultura áulica que propicia el análisis y la reflexión de los estudiantes a través de la incorporación de algunos protocolos, secuencias de preguntas y\/o pasos formulados para la exploración de ideas vinculados a los contenidos de la asignatura.","palabras_clave":"FORMACION|EDUCACION|DOCENTE|PENSAMIENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1442,"idioma_id":5,"titulo":"LA AGENDA DE PROBLEMAS DE UN SECTOR DOMINANTE LOCAL. SU EXPOSICIÓN EN EL PRIMER CONGRESO NACIONAL DEL COMERCIO ARGENTINO? -ROSARIO, 1911-","resumen":"Este trabajo explora la experiencia del Primer Congreso del Comercio Argentino desarrollado en Rosario en 1911. Nuesto objetivo es aproximarnos al problema de la sociabilidad empresaria y corporativa desde una doble entrada. Por un lado resaltar la configuración de este tipo de eventos en la clave de sociabilidades construidas desde bases institucionales que transitaban además intancias informales, y que se desarrollaban dentro límites temporales inmediatos. Por otro describir los elementos que se encontraban presentes en la elaboración de una agenda de temas compartidos por el grupo dominante rosarino y por sus pares regionales en el concierto nacional. El congreso fue una instancia singular y emergente de sólidas expresiones asociativas económicas que se manifestaban en el marco del estado liberal. Fue a su vez la imagen superadora de la rígidas figuras institucionales de las corporaciones económicas, al posibilitar otras formas de gestión y diálogo estamental.   Indexado en     SciELO, Redalyc;  Latindex ?Catalogo ?","palabras_clave":"Argentina|congresos|corporaciones|sociabilidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1444,"idioma_id":4,"titulo":"Determination of the moisture sorption behaviour of osmotically dehydrated mackerel fillets by means of binary and ternary solutions","resumen":"In this study the moisture sorption isotherm of osmotically dehydrated (OD) mackerel fillets (Scomber japonicus) was experimentally determined. The fillets were OD with solutions of salt (NaCl) (120 and 180 g per litre of solution) or in combination with sugar (350 to 700 g per litre of solution). The sorption isotherms were determined using the static gravimetric methodology with 6 salts for the water activity range of 0.33 to 0.98 at 5 and 25ºC. All the sorption curves were found to be Type III. Temperature and the final tissue salt content had significant (p<0.05) effects on the sorption isotherms. A regression program was used to fit the Halsey, the Oswin and the Smith moisture sorption isotherm models. The Oswin gave the best fit for the whole range of water activity and temperatures. The Smith equation only presented valuable results for the mackerel fillets samples with the higher salt content.","palabras_clave":"Scomber Japonicus|Salt-sugar solutions|Sorption isotherm|Modelling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1445,"idioma_id":5,"titulo":"La expansión de la infección malárica: Del norte subtropical a la región central. Argentina. 1900-1930.","resumen":"La aparición de focos de enfermedades transmitidas por la picaduras de mosquitos en zonas tan alejadas de las regiones conocidas en la literatura de la época como ?maláricas? alertó a las autoridades, pues por un lado, ponía en discusión una serie de postulados que vinculaban la presencia de la enfermedad a la cuestión climática, pero por otro al tratarse de una provincia densamente poblada las consecuencias podían ser mayores a las conocidas hasta entonces.    El nuevo cuadro de situación que la década del treinta trajo aparejado, con un Estado que desde los años veinte se venía perfilando como dirigista e interventor, (acciones que han sido analizadas por la historiografía argentina en el plano económico, en el social donde sobresalen los estudios en torno a la cuestión social  y en el político con las intervenciones a los gobiernos provinciales etc), induce a pensar que en materia de políticas sanitarias en general, pero en las rurales en particular se actuó con idéntica lógica de acción. Sin embargo, en las páginas siguientes se podrá observar como en materia de políticas públicas, por lo menos en las referidas a las rurales, hubo límites en el accionar de ese Estado que intentaba extender su gestión sanitaria. Esas  limitantes son explicadas en este paper por las vigencias de un sistema federalista de gobierno, donde las fronteras provinciales en varias ocasiones actuaron como muros de contención frente al avance del Estado Central y en otras se convirtieron en la única alternativa de acción  debido al accionar deficiente del Estado Central, lo cual era resultado de la inexperiencia que las agencias gubernamentales tenían en ese terreno. Esta perspectiva de análisis, traza un panorama sanitario donde se visualizan cambios, transformaciones pero también persistencias que le otorgan complejidad a un proceso en donde cobran protagonismo las respuestas regionales frente al avance del Estado Central, precisamente en un momento donde éste intentaba avanzar.","palabras_clave":"ENFERMEDAD|MALARIA|INSTITUCIONES SANITARIAS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1446,"idioma_id":5,"titulo":"JUEGO, REDES SOCIALES E INFANCIA: HACIA LA DEFINICIÓN DE NUEVOS  ESCENARIOS COMUNICATIVOS","resumen":"do por diversos investigadores a la luz de la cantidad de usuarios que poseen y por el peso significativo que tienen en un nuevo tipo de prácticas. Ahora bien, más allá de los usos generales de este fenómeno de la llamada Web 2.0, identificamos ?en el marco de un proyecto de investigación? un espacio en y desde el cual se producían interacciones dables de ser analizadas en profundidad: los videojuegos en las redes sociales. Los millones de usuarios que juegan diariamente a pasatiempos electrónicos ?es- pecíficamente en Facebook? ubican estas nuevas interacciones lúdicas como significativas para la cul- tura lúdica contemporánea y, por extensión, para la cultura contemporánea en términos generales. En este sentido, nos proponemos realizar aquí un análisis de los principales videojuegos de la red social Facebook. Buscaremos, de este modo, esbozar qué significados, temas e interacciones se favorecen (y obturan) en estos videojuegos. Jugar es una actividad profunda y para nada trivial: de allí la necesidad de analizar las representaciones y transformaciones que se dan","palabras_clave":"Juego|Redes Sociales|Nuevas tecnologías|Escenarios comunicativos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1447,"idioma_id":5,"titulo":"Interpretación de los hechos en la violencia de género.","resumen":"En los casos de violencia de género, para una adecuada y efectiva aplicación de los postulados constitucionales, instrumentos normativos y legislación nacional vigente en la materia, resulta esencial que los hechos del caso sean valorados con perspectiva de género, considerando entre otros factores, el impacto que este tipo de violencia genera en la personalidad y actitudes de la víctima.","palabras_clave":"VIOLENCIA|GÉNERO|CULTURA|AUTORITARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1448,"idioma_id":4,"titulo":"Biological activity of three alkyl cinnamates on young larvae of Tuta absoluta","resumen":"No dispongo del archivo","palabras_clave":"Lethal effects|Sublethal effects|treatment by immersion|topical treatment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1449,"idioma_id":5,"titulo":"La química verde como fuente de nuevos compuestos para el control de plagas agrícolas","resumen":"El uso masivo de plaguicidas sintéticos en la agricultura moderna ha desencadenado una serie de problemá-  ticas ambientales que comenzaron a visualizarse en la década del sesenta del siglo pasado y se agudizaron  con el tiempo. Las intoxicaciones de los trabajadores agrícolas, la presencia de residuos de plaguicidas en  los alimentos y su persistencia en el ambiente, la ruptura de los mecanismos de control biológico natural  y el desarrollo de resistencia en las poblaciones de plagas, son algunos de los efectos de los pesticidas  que atentan contra la salud humana y el ambiente. En las últimas décadas, diferentes reglamentaciones  ambientales han prohibido o limitado el uso de muchos de estos productos, incentivando la búsqueda de  nuevos compuestos selectivos y compatibles con el ambiente y de baja toxicidad hacia el hombre. En  este marco se presentan los postulados de la química verde, una forma de hacer química basada en la  sostenibilidad, tendiente a desarrollar métodos y procesos que eviten la generación de residuos en lugar  de hacer un tratamiento posterior de los mismos. En el presente trabajo se abordan y discuten los doce  principios ejempli&#64257;cados dentro del ámbito del control de plagas.","palabras_clave":"plaga,|plaguicidas,|química verde|sustentabilidad."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1450,"idioma_id":4,"titulo":"The Work, Perceptions and Professional Development of Teachers","resumen":"Silva, A. M. & Herdeiro, R. (2013). The Work, Perceptions and Professional Development of Teachers. Teaching Education. Routledge, 1-19. http:\/\/dx.doi.org\/10.1080\/10476210.2012.759554 Indexada","palabras_clave":"WORK|PERCEPTIONS|TEACHERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1451,"idioma_id":5,"titulo":"Balance de una generación. Continuidad de las estrategias de autolegitimación intelectual en la producción tardía de Manuel Ugarte","resumen":"Destacamos el balance ugarteano de la contribución histórica del modernismo hispanoamericano. El lugar que ocupa la llamada generación de Darío en la historia intelectual de América Latina es significativo por su papel determinante en la construcción de la figura del intelectual latinoamericano. La dinámica estatuida por la modernización continental a fines del siglo XIX y principios del XX promovió la autonomización del campo literario. Este hecho produjo una serie de modificaciones de la práctica de los intelectuales que operó como condición de posibilidad de un discurso que acentuó las expectativas acerca de la eficacia histórico-política de la escritura científica, filosófica y literaria. Analizamos los tópicos en torno de los cuales se construyeron aquellas expectativas a la luz del balance que realizara el escritor argentino Manuel Ugarte en dos textos tardíos como El dolor de escribir [1933] y Escritores iberoamericanos de 1900 [1943].","palabras_clave":"Balance|Ugarte|autolegitimación|generación del 900"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1452,"idioma_id":4,"titulo":"On the transferability of atomic contributions to the optical rotatory power of hydrogen peroxide, methyl hydroperoxide and dimethyl peroxide","resumen":"The chirality of molecules expresses itself, for example, in the fact that a solution of a chiral molecule rotates the plane of linear polarised light. The underlying molecular property is the optical rotatory power (ORP) tensor, which according to time-dependent perturbation theory can be calculated as mixed linear response functions of the electric and magnetic dipole moment operators. Applying a canonical transformation of the Hamiltonian, which reformulates the magnetic dipole  moment operator in terms of the operator for the torque acting on the electrons, the ORP of a molecule can be partitioned into atomic and group contributions. In the present work, we investigate the transferability of such individual contributions  in a series of small, chiral molecules: hydrogen peroxide, methyl hydroperoxide and dimethyl peroxide. The isotropic atomic or group contributions have been evaluated for the hydrogen, oxygen and carbon atoms as well as for the methyl group at  the level of time-dependent density functional theory with the B3LYP exchange-correlation functional employing a large Gaussian basis set. We find that the atomic or group contributions are not transferable among these three molecules.","palabras_clave":"CHIRALITY|OPTICAL ROTATORY POWER|ATOMIC CONTRIBUTIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1453,"idioma_id":5,"titulo":"La biopolítica: problematizaciones en torno  a la articulación teórico-empírica.","resumen":"Tomando como punto de partida los desarrollos teóricos de Michel Foucault   en relación con la noción de biopolítica (y su profundización en la de   dispositivos de seguridad), muchos han sido los autores que, desde diferentes   tradiciones y enfoques conceptuales, han recuperado la cuestión de la   relación entre política y vida, o más precisamente, del ejercicio del poder   sobre la vida. El presente artículo tiene como propósito hacer un aporte a   dicha reflexión a través de aplicar esta noción a una problemática empírica   nacional, de explorar un modo posible de articulación teórico-empírica,   tomando como objeto para tal finalidad el accionar del Ministerio Público   Fiscal. Esto partiendo del supuesto de que el concepto de biopolítica tiene   múltiples dominios de objetivación, tales como la sexualidad, la familia, la   escuela, etc., y campos de saberes desde los que ha sido abordado.","palabras_clave":"biopolítica |Ministerio Público Fiscal |grupo productor de riesgo|control"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1454,"idioma_id":4,"titulo":"?Ice loss monitoring in a covered glacier at the Aconcagua Region, Argentina by GPS semi-continuous station?,","resumen":"aaaa","palabras_clave":"Glaciologia|Geodesia|Geodinamica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1457,"idioma_id":4,"titulo":"URBAN PASSIVE COOLING. AGING EFFECTS ON OPTICAL PROPERTIES OF ROOF TILES","resumen":"When the percentage of solar radiation absorbed by the material of the urban envelope is diminished, its surface temperature can be reduced, thus the heat level is minimized. The application of reflective materials (high albedo) over the surfaces of building envelopes results in an effective passive cooling strategy. This work evaluates the influence of wear on the thermal performance of diverse roof tiles available in the region of Mendoza, Argentina. 16 roof tiles of different characteristics (colour, shape, composition and finish). The effect of aging was quantified by means of the Solar Reflectance Index of each material throughout a period of 3 years. The results obtained have been analyzed by relating the influence of different characteristics of the roof tiles to the thermal behavior of the material throughout time. They show that all the tested roof tiles tend to diminish their ability to reduce urban temperatures by 40%. 50% of clay compositions and 33% of concrete compositions show an SRI value of 30%, as a result of wear. ","palabras_clave":"Roof Tiles|Aging|Solar Reflectance Index|Material Features"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1458,"idioma_id":4,"titulo":"Representations of simple finite Lie conformal superalgebras of exceptional type $CK_6$.","resumen":"We classify all continuous degenerate irreducible modules over the exceptional linearly compact Lie superalgebra E(1, 6), and all finite degenerate irreducible modules over the exceptional Lie conformal superalgebra CK 6, for which E(1, 6) is the annihilation superalgebra.","palabras_clave":"superconformal algebras|representation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1459,"idioma_id":4,"titulo":"Synthesis and swelling behavior of pH-responsive polyurethane\/poly(2-(diethylamino)ethyl methacrylate) hybrid materials","resumen":"Polyurethane (PU)\/poly[2-(diethylamino)ethyl methacrylate] hybrids, having a chemical bond between the PU and acrylic moieties and with different compositions, were prepared by the dispersion polymerization of 2-(diethylamino)ethyl methacrylate (DEA) in the presence of preformed PU chains with polymerizable terminal vinyl groups. The PU dispersion was synthesized according to a prepolymer mixing process by the polyaddition of isophorone diisocyanate, poly(propylene glycol), 2-hydroxyethyl methacrylate, and dimethylol propionic acid (DMPA). Then, it was dispersed in water by the prior neutralization of the carboxylic acid groups of DMPA with triethylamine, chain-extended with ethylenediamine. The effect of the DEA content on the swelling properties (water uptake and dynamic swelling degree) at different pHs and at 37°C was determined. The samples were also characterized by Fourier transform infrared spectroscopy and modulated differential scanning calorimetry. The experimental results indicate a higher water uptake when the DEA content was increased on the hybrid materials and a significant change in the kinetics of swelling at pH 4 compared to those at pH 7. The water content of the hydrogels depended on the DEA content, and it was inversely proportional to the pH value. The pure PU film did not show important changes over the pH range examined in this study. The synthesized hybrids were useful as drug-delivery, pH-sensitive matrices.","palabras_clave":"drug-delivery systems| PUs| stimuli-sensitive polymers|swelling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1460,"idioma_id":4,"titulo":"DEVELOPING A THERMAL COMFORT INDEX FOR VEGETATED OPEN SPACES IN CITIES OF ARID ZONES","resumen":"The degree of thermal comfort that people experience in open spaces is one of the determinants for use, especially in areas with extreme climates. It has a wide range of indices in the international literature. However, the study of five indices (THI, PE, TS, PMV and COMFA) regarding subjective responses presented low percentages of predictive successes in the city of study, characterized by an arid climate and intense urban vegetation. Therefore, the aim of this work is to develop a new thermal comfort index to predict more accurately the thermal sensation of the local residents. For this, there were campaigns monitoring of microclimatic parameters and field surveys about the real sensation of the people on a pedestrian street of Mendoza Metropolitan Area (MMA) in both winter and summer. The proposed new model is established through the correlation of environmental variables and individual subjective responses. The multivariate correlation is performed by linear regression. The theory of Akaike´s information is used as criterion to model selection. The new model IZA is a lineal relation of air temperature, relative humidity, wind speed and solar radiation, generally all available weather variables. We found a high correlation between subjective responses and the new model. Besides the Pearson coefficient which gives the new index is higher than other traditional indices evaluated. The percentage of success of the new index exceeds 85%, demonstrating the effectiveness of the proposed model. In conclusion, the use of IZA as a predictor of thermal comfort will allow a fair assessment of the effect of design and composition of space on your comfort conditions, without the need to carry out thermal perception surveys. This will facilitate the evaluation and decision-making regarding the thermal rehabilitation of open spaces.","palabras_clave":"THERMAL COMFORT|OPEN SPACES|OBJECTIVE METHODS|SUBJECTIVE METHODS|ADAPTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1461,"idioma_id":5,"titulo":"POLITICAS DE INCLUSION EN LA FORMACIÓN DOCENTE: LAS TRAYECTORIAS CULTURALES DE LOS ALUMNOS EN LOS INSTITUTOS DE FORMACIÓN","resumen":"El aumento progresivo de políticas de inclusión, desplegadas por los sistemas educativos nacionales en las últimas décadas, contemplaron principalmente a los niveles elementales de educación buscando elevar los índices de retención y permanencia. La trayectoria ideal de los alumnos, podía medirse en términos de inicio y culminación de cada nivel; paulatinamente el diseño y desarrollo de acciones para el nivel superior siguieron esta lógica.  La inclusión se transformó en un concepto, que intentó mejorar aspectos de la calidad, promoviendo la retención y la permanencia. Actualmente inclusión y calidad exigen mirar también el tipo de experiencia que se ofrece a los alumnos, reconociendo nuevas formas de exclusión, aun estando en el sistema.  Nuestro trabajo presenta un análisis de las políticas de inclusión en la formación docente, centrando la mirada en las trayectorias culturales de los sujetos, entendiendo que es un elemento fundamental para la comprensión del logro efectivo o no de las políticas de inclusión educativa.  Este artículo se basa en datos obtenidos en el marco de investigaciones financiados por el INFoD y el Ministerio de Educación de la provincia de Salta para la investigación en Institutos de Formación Docente.  La indagación recopila información de cinco instituciones de capital e interior de la provincia, a partir del análisis bibliográfico y documental, entrevistas en profundidad y aplicación de cuestionarios desde una lógica cuanti-cualitativa, con la finalidad de describir y problematizar la atención a las trayectorias culturales de los alumnos en los Institutos de Nivel Superior","palabras_clave":"Formación  Docente |Trayectorias Culturas|Políticas de Inclusión"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1463,"idioma_id":5,"titulo":"Lógica de acción y relaciones de los sindicatos estatales con el gobierno de Néstor Kirchner (2003-2007).","resumen":"En el presente trabajo se analiza el devenir de la relación de los sindicatos estatales nacionales con el gobierno de Néstor Kirchner entre 2003 y 2007. A partir de la articulación de características propias del campo de actuación y de los actores intervinientes se  destaca el proceso que llevó a revertir las alianzas y disputas iniciales entre cada uno de los gremios y el gobierno. El abordaje metodológico es eminentemente cualitativo, a través de entrevistas en profundidad a dirigentes y delegados sindicales, así como a trabajadores y funcionarios.","palabras_clave":"Sindicatos|gobierno|relaciones laborales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1464,"idioma_id":4,"titulo":"The Knowability Argument and the syntactic type-theoretic approac","resumen":"Some attempts have been made to block the Knowability Paradox and other modal paradoxes by adopting a type-theoretic framework in which knowledge and necessity are regarded as typed predicates. The main problem with this approach is that when these notions are simultaneously treated as predicates, a new kind of paradox appears. I claim that avoiding this paradox either by weakening the Knowability Principle or by introducing types for both predicates is rather messy and unattractive. I also consider the prospect of using the truth predicate to emulate other modal notions. It turns out that this idea works quite well.","palabras_clave":"Knowability Argument|Type-theoretic approach|Self-reference|Multimodal Paradoxes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1465,"idioma_id":4,"titulo":"Bremsstrahlung in carbon thick targets by proton incidence","resumen":"The subtraction of the continuum from an x-ray spectrum emitted by proton bombardment is usually carried out by means of a mathematical fitting. The purpose of the present work is to develop an analytical function to model the continuous spectrum generated in a PIXE experiment for different incident beam energies in carbon thick targets. With this purpose, PIXE spectra of a carbon bulk sample were measured in an ion accelerator. The proton beam energies were varied between 0.7 MeV and 2 MeV and the x-rays generated were collected by an energy dispersive spectrometer. The spectra analysis was performed taking into account the main effects underlying the production of the continuous spectrum. Nevertheless, for the cases considered here, it was found that the atomic bremsstrahlung is the most important and other contributions were neglected. The experimental spectra from carbon thick targets were corrected by self-absorption and detector efficiency. The results show that the spectral shape corresponding to thick targets corrected by these effects is similar to the functional behavior presented by thin targets.","palabras_clave":"X-ray bremsstrahlung|Proton impact|PIXE|Atomic bremsstrahlung"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1467,"idioma_id":5,"titulo":"Comentario de The sociology of War and Violence de Sinisa Malesevic","resumen":"?The sociology of War and Violence? de Sini?a Male?evi&#263; (2010) constituye un trabajo sociológico e histórico consistente que se propone introducir a la violencia organizada y la guerra como objetos de estudio en sí mismos, constitutivos e inseparables  de la posibilidad de lo social. Preguntándose por la persistencia e incremento de la violencia en la modernidad recorre los aportes clásicos y contemporáneos de la sociología, y revisa los aportes de los teóricos sociales ?militaristas? del siglo XIX soslayados o pacificados por las relecturas posteriores a la segunda guerra mundial. Los conceptos centrales de burocratización acumulativa de la coerción e ideologización masiva centrífuga, son puestos en juego en el análisis del nacionalismo y la propaganda de guerra para lograr exponer la mecánica de la violencia como constitutiva del lazo social. De esta manera Male?evi&#263; logra reunir argumentos y evidencias para sostener una propuesta anti-ilustrada, pero netamente sociológica y contemporáneamente relevante.","palabras_clave":"SOCIOLOGÍA DE LA GUERRA|VIOLENCIA ORGANIZADA|IDEOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1468,"idioma_id":5,"titulo":"?Paisajes arqueofaunísticos?: distribución y explotación diferencial de ungulados en el sector centro-oriental de la región pampeana","resumen":"Se analiza la representación arqueológica de cuatro especies de ungulados (Lama guanicoe, Blastocerus dichotomus, Ozotoceros bezoarticus y Mazama gouazoubira) explotados por las poblaciones humanas que habitaron el sector centro-oriental de la Región pampeana en los últimos 2500 años C14 AP. Se considera la evolución del ambiente y los requerimientos ecológicos que posee cada especie, con el objetivo de discutir diferentes problemas relacionados con su disponibilidad espacial y temporal, su importancia económica, y su representación diferencial en las distintas áreas y sitios arqueológicos que se encuentran en la región bajo estudio.","palabras_clave":"DISTRIBUCIÓN DE UNGULADOS; HOLOCENO TARDÍO; ZOOARQUEOLOGÍA DE CAZADORES-RECOLECTORES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1470,"idioma_id":4,"titulo":"AMELOBLASTOMA, HPV AND P53","resumen":"Ameloblastomas are odontogenic tumors with an aggressive local behavior. While&nbsp; Human Papilloma Virus(HPV)&nbsp; is often identified in various oral pathologies of the soft tissue, there are studies associate in with ameloblastomas, having detected it in 30 to 60 % of cases. it has been postulated that the gene p53 (phosphoprotein) in one of the `polymorphic forms would increase the risk of malignant transformation in neoplasms in witch HPV is identified.","palabras_clave":"AMELOBLASTOMA|HPV|P53|ORAL PATHOLOGIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1471,"idioma_id":4,"titulo":"PROTOONCOGENE C-MYC IN BUCCAL MUCOSA. OVEREXPRESSION","resumen":"The superfamily c-myc would be associated with different neoplasms. C-myc is amplified when the cell proliferation index is high. For some epithelial tumors, agressiveness and risk of metastasis would increase when there is overexpression of c-myc. The objective of this work was to study the amplification and\/or overexpression of the protoncogene c-myc in bening proliferative lesions and cancer located in buccal mucosa.","palabras_clave":"PROTOONCOGENE C-MYC|BUCCAL MUCOSA|OVEREXPRESSION|NEOPLASMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1473,"idioma_id":5,"titulo":"?Una sociología moral de las relaciona bancarias?","resumen":"En la última década se ha producido un creciente interés en la sociología, la antropología y la historia por el estudio de las finanzas. En E.E.U.U. y Europa se han editado libros, organizado coloquios, dossiers en revistas especializadas, grupos de investigación e incluso un área disciplinar especifica (social studies of finance). La crisis de las subprime en 2008 le ha dado un nuevo impulso a este tópico en la sociología económica (Carruthers y Kim, 2011). En nuestra región, también hemos sido testigos (y partícipes) de la emergencia de una agenda de investigación centrada en interrogar las relaciones y prácticas financieras formales e informales, licitas e ilícitas, tradicionales y nuevas (Villareal, 2009; Müller, 2009; Wilkis, 2010; Ossandon, 2011).   A riesgo de simplificar, podemos ver que la literatura especializada se ha ido organizado en los siguientes tópicos: el análisis de la socio-historia de los instituciones, instrumentos y saberes financieros; el estudio de las técnicas y tecnologías de evaluación crediticia; el estudio de los conocimientos económicos ordinarios y las estrategias económicas de los hogares en relación a una oferta extendida y diversificada de instrumentos financieros; el estudio de las finanzas alternativas (Maurer, 2012).   \tEl trabajo de Jeanne Lazarus (L?épreuve de l?argent. Banques, banquiers, clients, Calman Lévy, 2012) es una pieza en este ordenamiento del conocimiento de las ciencias sociales sobre las instituciones, las prácticas y los saberes centrales del capitalismo cuyo rasgo dominante es la financiarización (Lapavitzas, 2009).","palabras_clave":"credito|relación bancaria|sociología moral"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1474,"idioma_id":4,"titulo":"Inter-group guanaco variability in Southern Patagonia: carbon stable isotopes analyses and its implications for zooarchaeological studies","resumen":"We study the isotopic variability of modern social groups of guanacos (Lama guanicoe) and discuss the implications of these results for the analysis of archaeofaunas from archaeological sites of Patagonia. The aim of this work is to evaluate whether the study of the isotope composition of carbon and nitrogen from collagen (delta 13C and delta 15N) is a methodology that allows the discussion of hunting strategies-individual versus massive-carried out by human populations in this particular case of the guanaco from southern Patagonia. Samples come from five modern assemblages of guanacos located in the west margin of the Cardiel Lake and are the result of a catastrophic mortality episode produced by winter stress. The isotopic variability of these samples is compared with that of a mesoregional attritional assemblage built from multiple sites and chronologies. The results indicate, in the first place, that there is no differentiation between males and females, second, that the offspring show no increase of signals when compared to the other age categories and finally, juveniles recorded the lowest delta 15N values. In relation to the main purpose of this research, measurements of variability from modern assemblages and the attritional groups have been compared. The three assemblages with lower variability are modern ones. However, the two remaining modern assemblages record a variability higher than or equal to the attritional groups. Thus, for southern Patagonian guanacos, the hypothesis that proposes that the isotopic variability of a herd would be lower than the one obtained from multiple populations and different hunting events is rejected. Finally, we analyze the possible causes for these results together with situations in which delta 13C and delta 15N can be used in the study of the guanaco hunting strategies in southern Patagonia.","palabras_clave":"Guanaco|Holocene|Patagonia|Hunter-Gatherer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1476,"idioma_id":5,"titulo":"La filosofía marxista entre Francia y América Latina: una lectura de la correspondencia entre Louis Althusser y Fernanda Navarro.","resumen":"En 1988, fue publicado por Siglo XXI México Filosofía y marxismo, un pequeño libro de entrevistas al filósofo marxista francés Louis Althusser realizadas por la profesora de filosofía mexicana Fernanda Navarro. En dicho volumen, Althusser retomaba varios de los aspectos fundamentales que habían caracterizado su intervención durante las décadas de 1960 y 1970 e introducía elementos absolutamente novedosos como las formulaciones alrededor del materialismo aleatorio y la filosofía del encuentro. Del prefacio escrito por Althusser y la introducción realizada por Navarro, se desprendía que el origen del libro se remontaba a un encuentro azaroso entre la mexicana y el francés ocurrido hacia 1984, y que la publicación del libro estaba dirigida a los estudiantes y militantes de ?América Latina, exclusivamente? y que no sería editado en Francia. La correspondencia mantenida entre Althusser y Navarro entre la entrevista de 1984 y la publicación de Filosofía y marxismo en 1988, habilita un horizonte interpretativo que permite integrar el vínculo establecido entre ambos filósofos en el marco más general del itinerario teórico-político de Althusser en Francia y de las proyecciones del althusserianismo hacia América Latina. De este modo, veremos de qué manera aquel encuentro entre Althusser y Navarro estuvo originado en un intercambio epistolar previo entre el filósofo francés y Mauricio Malamud, un comunista argentino difusor de la obra de Althusser en Argentina y por entonces exiliado en Morelia. Asimismo, la correspondencia entre Althusser y Navarro nos permite ver que el hecho de que el libro haya sido publicado únicamente en América Latina fue resultado de un interés específico de Althusser en los procesos políticos latinoamericanos. Finalmente, el intercambio permite captar el tenor filosófico del mencionado libro en el marco más general de la especificidad de la intervención althusseriana.","palabras_clave":"Louis Althusser|Fernanda Navarro|Marxismo|Correspondencia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1477,"idioma_id":4,"titulo":"Ordovician sponge spicules from Spitsbergen, Nevada and Newfoundland: new evidence for hexactinellid and demosponge early diversification.","resumen":"Unusually well-preserved spicule assemblages from three Lower to Middle Ordovician localities are examined:  Spitsbergen (Floian-Dapingian), Nevada (Vinini Formation, Dapingian) and Newfoundland (Cow Head Group,  Tremadocian-lower Darriwilian, and Table Head Group, mid-Darriwilian). The recorded diversity increases the knowledge  of sponge spicules in the Lower Ordovician and their palaeogeographical distribution. Hexaster type microscleres and  scopules, equinate hexactins, pinular hexactins and inflated pentactins and hexactins are the main spicule types amongst  the Hexactinellida. Hemidiscs and a possible amphidisc are recorded and can be evidence of the presence of the  hexactinellid subclass Amphidiscophora. Oxyasters, C-shaped sigmata and trianes are the main spicule types amongst  demosponges. The presence of sigmata and oxyasters can also be evidence of the presence of two main orders of extant  demospongids: Sigmatophora (now Spirophorida) and Astrophora. One modified octactinellid spicule type is found from  Calcarea. The presence of echinate and pinulate hexactins amongst megascleres suggests a protective functional  morphology. These features can be associated with an evolutionary tendency which is seen mainly amongst hexactinellids  and demosponges for an efficient occupation of shallower settings and\/or a defensive armoury strategy. A newly recorded  flat-top pinulate form is also a suitable element for reinforcement or protection of any external surface. Some of the  microscleres found can be considered ancient homologues of recent hexasters, sceptrules, hemi\/amphidiscs and oxyasters.  This work suggests that the microscleres of hexactinellid and demosponge subclasses were already present and  significantly diversified in the Early Ordovician. The presence of definite axial canals in scopules provides key evidence  for this statement. This finding is significant and confirms the presence of the Sceptrulophora in the early Palaeozoic. The  microscleres comes from widely separated Lower Ordovician localities and possess similar forms to those found in  Mesozoic microscleres, implying a conservative morphology.","palabras_clave":"ORDOVICIAN|SPONGE|SPICULES|EARLY DIVERSIFICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1478,"idioma_id":4,"titulo":"Structural and magnetic study of zinc- doped magnetite    nanoparticles and ferrofluids for hyperthermia applications","resumen":"Cubic-like shaped ZnxFe3&#8722;xO4 particles with crystallite mean sizes D between 15 and 117 nm were obtained by co-precipitation. Particle size effects and preferential occupation of spinel tetrahedral site by Zn2+ ions led to noticeable changes of physical properties. D &#8805; 30 nm particles displayed nearly bulk properties, which were dominated by Zn concentration. For D &#8804; 30 nm, dominant magnetic relaxation effects were observed by Mössbauer spectroscopy, with the mean blocking size DB ~ 13 to 15 nm. Saturation magnetization increased with x up to x ~ 0.1\u20130.3 and decreased for larger x. Power absorbed by water and chitosan-based ferrofluids from a 260 kHz radio frequency field was measured as a function of x, field amplitude H0 and ferrofluid concentration. For H0 = 41 kA m&#8722;1 the maximum specific absorption rate was 367 W g&#8722;1 for D = 16 nm and x = 0.1. Absorption results are interpreted within the framework of the linear response theory for H0 &#8804; 41 kA m&#8722;1. A departure towards a saturation regime was observed for higher fields. Simulations based on a two-level description of nanoparticle magnetic moment relaxation qualitatively agree with these observations. The frequency factor of the susceptibility dissipative component, derived from experimental results, showed a sharp maximum at D ~ 16 nm. This behaviour was satisfactorily described by simulations based on moment relaxation processes, which furthermore indicated a crossover from Néel to Brown mechanisms at D ~ 18 nm. Hints for further improvement of magnetite particles as nanocalefactors for magnetic hyperthermia are discussed.","palabras_clave":"SOFT MATER|MAGNETIC|BIOLOGICAL PHYSICS|NANOSCALE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1480,"idioma_id":4,"titulo":"Pharmaceutical optimization of lipid-based dosage forms for the improvement of taste-masking, chemical stability and solubilizing capacity of phenobarbital","resumen":"Microemulsions (MEs) and self-emulsifying drug delivery systems (SEEDS) containing phenobarbital(Phe) were developed to improve its chemical stability, solubilizing capacity andtaste-masking in oral liquid dosage forms. Cremophor RH40 and Labrasol were used assurfactants for the screening of ME regions, Capmul MCM L, Captex 355, Imwitor 408,Myglyol 840 and Isopropyl myristate were the oil phases assayed; Transcutol P, Polyethyleneglycol400, glycerol, Propylene-glycol and ethanol the cosurfactants. Phe stability assay wascarried out (20:4:20:56% and 20:4:35:41% (w\/w); surfactant:oily phase:cosurfactant:water) forboth surfactants; only one containing ethanol showed significant dismissing in its drug content.Solubility capacity for these selected formulations were also evaluated, an amount between17 and 58 mg\/mL of Phe could be loaded. At last, an optimized ME formulation withCremophor RH40 20%, Capmul MCM L 4%, PEG 400 35% and sucralose 2% (w\/w) was chosenin order to optimize taste-masking using an electronic tongue. Strawberry along with bananaand tutti-frutti flavors plus mint flavor proved to be the best ones. Labrasol-based preconcentrateswere tested for (micro)emulsifying properties; all of them resulted to behave asSEDDS. In summary, a rationale experimental design conducted to an optimized ME for Phe oralpediatric administration which was able to load 5-fold times the currently used dose (4 mg\/mL),with no sign of physical or chemical instability and with improved taste; SEDDS for capsulefilling were also obtained. The biopharmaceutical advantages described for these dosage formsencourage furthering in vivo evaluation.","palabras_clave":"Biopharmaceutical advantages|chemical degradation|electronic tongue|microemulsion|SEDDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1482,"idioma_id":5,"titulo":"Mecanismo de acción de las gonadotrofinas sobre el ovario bovino y su participación en la enfermedad quística ovárica.","resumen":"Las gonadotrofinas son esenciales para la función  ovárica del bovino actuando, a través de la interacción con sus receptores, en  la regulación del crecimiento y la maduración folicular así como en la  esteroidogénesis. Se postula que cualquier modificación en los mecanismos de transcripción  y\/o regulación de dichos receptores podría ser un factor desencadenante de diferentes  alteraciones reproductivas entre las que se encuentra la enfermedad  quística ovárica (COD). La COD se define como la interrupción de los ciclos  estrales normales debido a una falla en la ovulación y consecuente persistencia  de un folículo que aumenta de tamaño superando el diámetro ovulatorio. Se ha  demostrado que los receptores de gonadotrofinas presentan bajos niveles de expresión  en ovarios enfermos en relación a ovarios sanos. Además, se han identificado  una variedad de isoformas de las gonadotrofinas que podrían ejercer  un rol significativo en la regulación de la expresión de los receptores  funcionales e intervenir en la patogenia de la COD, siendo además, una  probable causa de la falta de respuesta a los tratamientos. ","palabras_clave":"RECEPTORES DE GONADOTROFINAS|BOVINOS|ENFERMEDAD QUÍSTICA OVÁRICA|GONADOTROFINAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1483,"idioma_id":4,"titulo":"Seasonal density and distribution of Octopus tehuelchus in the intertidal of North Patagonia","resumen":"The abundance and distribution of coastal cephalopods are strongly influenced by the environmental conditions. This study  evaluates the seasonal distribution and density of Octopus tehuelchus in three intertidal environments of San Mat&#305;as Gulf  (Patagonia). To estimate and compare density and distribution, monthly transects were carried out, and analysed in relation  to the coastlines obtained with satellite images. Densities of O. tehuelchus ranged between 0 and 34 individuals per transect  (0-0.11 octopus m-2), and varied between sites. Islote Lobos (IL) had the highest densities (mean octopus per transect 7.32+  1.16) followed by El Fuerte (EF) (5.55 +0.94) and San Antonio Bay (SAB) (3.40 +0.85). Within each site, octopus densities  varied between seasons. The highest densities were observed during warm months (SAB = 5.96, EF = 9.52, IL = 14.15) and  the lowest during cold ones (SAB = 1.45, EF = 2.6, IL = 3.32). Octopus tehuelchus showed an aggregated spatial distribution,  and the nearest neighbour value varied between 0.21 and 0.83 in SAB, 0.27 and 0.78 in EF and 0.23 and 0.53 in  IL. Considering the coast lines, the distribution pattern along the intertidal varied seasonally. During warm months this  species is widely distributed throughout the intertidal, from the low-tide line to 300 m (in SAB), 500 m (in EL) and 200 m  (in IL). On the contrary, during cold months most individuals were found near the low-tide line. These results may be associated  with the availability of shelter in the intertidal and the activity and physiology of this species.","palabras_clave":"Density|Distribution|Octopus tehuelchus|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1484,"idioma_id":4,"titulo":"Synaptic control of local translation: the plot thickens with new characters.","resumen":"The production of proteins from mRNAs localized at the synapse ultimately controls the strength of synaptic transmission, thereby affecting behavior and cognitive functions. The regulated transcription, processing, and transport of mRNAs provide dynamic control of the dendritic transcriptome, which includes thousands of messengers encoding multiple cellular functions. Translation is locally modulated by synaptic activity through a complex network of RNA-binding proteins (RBPs) and various types of non-coding RNAs (ncRNAs) including BC-RNAs, microRNAs, piwi-interacting RNAs, and small interference RNAs. The RBPs FMRP and CPEB play a well-established role in synaptic translation, and additional regulatory factors are emerging. The mRNA repressors Smaug, Nanos, and Pumilio define a novel pathway for local translational control that affects dendritic branching and spines in both flies and mammals. Recent findings support a role for processing bodies and related synaptic mRNA-silencing foci (SyAS-foci) in the modulation of synaptic plasticity and memory formation. The SyAS-foci respond to different stimuli with changes in their integrity thus enabling regulated mRNA release followed by translation. CPEB, Pumilio, TDP-43, and FUS\/TLS form multimers through low-complexity regions related to prion domains or polyQ expansions. The oligomerization of these repressor RBPs is mechanistically linked to the aggregation of abnormal proteins commonly associated with neurodegeneration. Here, we summarize the current knowledge on how specificity in mRNA translation is achieved through the concerted action of multiple pathways that involve regulatory ncRNAs and RBPs, the modification of translation factors, and mRNA-silencing foci dynamics.","palabras_clave":"SYNAPTIC CONTROL|LOCAL TRANSLATION|mTOR|NMDAR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1485,"idioma_id":4,"titulo":"A circular microchannel with integrated electrodes for DNA electrophoresis","resumen":"The development of a novel microfluidic device that includes a circular microchannel and integrated electrodes for DNA electrophoresis is reported. The geometry of the separation channel and the arrangement of the embedded electrodes provide several advantages in relation to conventional linear microchannels. The paper describe the design, modeling, construction, and testing of the micro device. Numerical simulations were used to investigate the electric field into the microchannel. In addition, the electrophoretic transport of DNA samples was studied under different voltage configurations. The experiments reported show the functionality of the device, and illustrate interesting features of DNA transport.  ","palabras_clave":"MICROCHANNELS|ELECTROPHORESIS|DNA|BIOTRON"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1486,"idioma_id":5,"titulo":"Presiones Fiscales y Conflicto Político en Argentina: La Intensificación de las Tensiones entre un Primus y sus Pares","resumen":"Durante el 2013, el gobierno central y sobre todo las provincias en Argentina enfrentaron crecientes presiones fiscales. Si bien el contexto económico repercutió en los votos recibidos por el oficialismo en las elecciones legislativas, esto no alteró sustancialmente la composición de fuerzas en el Congreso. Con apoyo legislativo, la presidenta pudo avanzar su agenda legislativa. Con presiones fiscales crecientes, los gobernadores han profundizado los esfuerzos de coordinación frente al gobierno central. Esto hace que una de las características centrales de la política argentina durante 2013 haya sido la intensificación del conflicto del presidente (un primus) contra los gobernadores (sus pares).","palabras_clave":"Elecciones en Argentina|Gobernadores|Presidente|Finanzas Públicas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1487,"idioma_id":4,"titulo":"Pd-based binary and ternary alloy membranes: morphological and perm-selective characterization in the presence of H2S","resumen":"Pd, Pd90Ag10, Pd91Au9, Pd78Ag9Au13 and Pd75Ag16Au9  alloy membranes were prepared on vacuum-assisted ZrO2-modified porous stainless steel supports by sequential electroless deposition.  The membranes were evaluated for permeability in pure H2 and in an H2S\/H2  mixture. The membranes displayed a range of permeabilities in pure H2:  Pd90Ag10 &gt; Pd78Ag9Au13&gt;  Pd ~ Pd75Ag16Au9 &gt;Pd91Au9. Upon exposure to 100 ppm H2S\/H2 at 673 K for 24  hours, all membranes lost a significant fraction of their H2permeabilities:  Pd (lost the largest fraction, 85%) &gt; Pd90Ag10&gt; Pd75Ag16Au9&gt;  Pd78Ag9Au13&gt;Pd91Au9  (60%).&nbsp; When H2S  was removed, the membranes recovered the lost permeability to different  extents. The Pd91Au9 and Pd78Ag9Au13  membranes displayed the highest fractional recovery of initial H2 permeability  (~ 80 %).&nbsp; But, with its higher initial  pure H2 permeability, Pd78Ag9Au13  had the highest absolute H2 permeability after recovery. The  microstructure, morphology and bulk composition of H2S-exposed  samples were characterized by X-ray diffraction (XRD), scanning electron  microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). XRD revealed the presence of a bulk Pd4S phase on both Pd  and Pd90Ag10, the membranes that recovered the least H2  relative flux after their exposure to H2S. In contrast, bulk  sulfides did not form on Pd91Au9 or on PdAgAu ternary  alloys. In agreement with the XRD results, EDS did not detect sulfur in the  bulk of Pd91Au9 or in either of the ternary alloy  samples. Our results show that the addition of Au to the high-permeability PdAg  binary membrane results in a PdAgAu ternary membrane that minimizes the  permanent H2 flux loss associated with H2S exposure by  preventing the formation of thick stable sulfides.","palabras_clave":"Pd|sulfur effect"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1488,"idioma_id":4,"titulo":"A polycaprolactone-based compatibilization treatment to improve dispersion and interphase structure of silica polyurethane composites","resumen":"Silica nanoparticles  (SN) were grafted with e-caprolactone using an environmentally friendly  approach. By using tartaric acid as a catalyst and the silanol groups as  initiators, grafted nanoparticles (GN) with organic weight fractions (wof) in the range 0-46 wt.%  were synthesized. Thermogravimetric weight loss (TGA) and infrared analysis were  used to measure the wof  and to corroborate the covalent bond between the silica nanoparticle and the  caprolactone monomer. The GNs were used to prepare polyurethane (PU) nanocomposites.  Transmission Electron Micrographs (TEM) corroborated that the GNs interfacial  area with the PU increased by the reduction of agglomerate dimensions from 10mm to around 100nm. Dynamic Mechanical Analysis,  Differential Scanning Calorimetry (DSC), X-Ray Diffraction, TGA and Tensile  tests were used to evaluate the effect of changing wof and filler concentration on the nanocomposite  properties. At low wof (&lt;15%),  the GNs improved Elastic Modulus (), Strength () and Ductility (). At higher  wof (&lt;15%), the properties of the nanocomposite tended to change. The  thermal stability of the GN based PU nanocomposites was improved, mainly by  increasing the stability of the castor oil based soft segments","palabras_clave":"NANOCOMPSOSITE|POLYURETHANE|SILICE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1489,"idioma_id":4,"titulo":"Sums of variables at the onset of chaos","resumen":"We explain how specific dynamical properties give rise to the limit distribution of sums of deterministic variables at the transition to chaos via the period-doubling route. We study the sums of successive positions generated by an ensemble of initial conditions uniformly distributed in the entire phase space of a unimodal map as represented by the logistic map. We find that these sums acquire their salient, multiscale, features from the repellor preimage structure that dominates the dynamics toward the attractors along the period-doubling cascade. And we explain how these properties transmit from the sums to their distribution. Specifically, we show how the stationary distribution of sums of positions at the Feigebaum point is built up from those associated with the supercycle attractors forming a hierarchical structure with multifractal and discrete scale invariance properties.","palabras_clave":"limit distribution |period-doubling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1490,"idioma_id":4,"titulo":"End-point estimates for iterated commutators of multilinear singular integrals","resumen":"Iterated commutators of multilinear Calder &#769;on-Zygmund operators and pointwise multiplication with functions in BMO are studied in products of Lebesgue spaces. Both strong type and weak end-point estimates are obtained, including weighted results involving the vectors weights of the multilinear Calderón-Zygmund theory recently introduced in the literature. Some better than expected estimates for certain multilinear operators are presented too.","palabras_clave":"Commutators|multilienear operators|Ap weights"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1491,"idioma_id":5,"titulo":"Algunas reflexiones para analizar la gubernamentalidad neoliberal  y a quienes la impugnan","resumen":"Resumen:  En este escrito nuestro objetivo es reflexionar acerca del ejercicio del poder que impone el neoliberalismo. Para ello seguimos algunas de las pistas que nos ofrece M. Foucault desde una perspectiva crítica, en función del análisis sobre las transformaciones acaecidas en el dominio de los cuerpos, el territorio y la población en Latinoamérica, en las últimas décadas. A esta nueva colonización expropiatoria (de las energías del cuerpo, los bienes comunes y la ciudadanía social), se le enfrentan, diversos modos de recusación o contraconductas que a su modo impugnan la gestión neoliberal, planteando un desafío central: el dilema entre sólo- la- vida, umbral último de sobrevivencia de la población y la vida digna de ser vivida, aquella que se funda en las necesidades de cada comunidad. La biopolítica neoliberal en este sentido, actualiza la confrontación sobre ¿cómo vivir?, ¿con qué vivir?, ¿para quién o para qué vivir?","palabras_clave":"NEOLIBERALISMO|CONFLICTO SOCIAL|MOVIMIENTOS SOCIALES|RESISTENCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1492,"idioma_id":5,"titulo":"Las canteras de Doncellas. Caracterización de Fuentes de aprovisionamiento y materias primas líticas del Departamento de Cochinoca (Jujuy, Argentina).","resumen":"Se presenta la caracterización de cinco fuentes de aprovisionamiento de materia prima lítica identificadas en la localidad de Doncellas (Departamento de Cochinoca, Jujuy), y un estudio comparativo de los resultados de la caracterización por medio de análisis petrográfico en cortes delgados entre ocho muestras de rocas. Cinco de ellas corresponden a muestras tomadas en las fuentes, mientras que otras tres provienen de los sitios arqueológicos Doncellas (o Agua Caliente de Rachaite), Sayate y Sorcuyo (o Pueblo Viejo de Tucute). Los resultados alcanzados en los análisis efectuados en muestras arqueológicas y de fuentes de aprovisionamiento indican que, si bien estamos en presencia de dos tipos de rocas volcánicas: andesitas y basaltos, en ambos casos la variabilidad que presentan permiten discriminar cuatro grupos en base a sus características petrográficas. A partir de la caracterización de los materiales se busca entender y explicar la variabilidad de los conjuntos artefactuales y la oferta de recursos locales.","palabras_clave":"COCHINOCA|MATERIALES|LITICOS|PROVENIENCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1494,"idioma_id":4,"titulo":"The value of impulsivity to define subgroups of addicted individuals differing in personality dysfunction, craving, psychosocial adjustment and wellbeing: A latent class analysis","resumen":"Background: High impulsivity is trans-diagnostically associated with several addiction-related disorders including pathological gambling, substance dependence and personality disorders. Here we applied latent class analysis to test the value of impulsivity indices to identify subgroups of addicted individuals that may entail different diagnostic compositions and differ in addiction-related outcomes. Methods: A sample diagnosed with cocaine dependence (with and without personality disorders from Cluster B and Cluster C) and pathological gamblers were recruited. We applied latent class analyses to the scores of participants on the UPPS-P impulsive behavior scale consisting on five impulsivity personality pathways (Sensation Seeking, Lack of Perseverance, Lack of Premeditation, Negative Urgency, and Positive Urgency) to measure trait impulsivity, and the Stroop Color-Word Interference Test and d2 Cancellation Test to measure cognitive impulsivity. The resulting Classes were compared in terms of diagnostic composition, estimates of substance use behavior and personality dysfunction, and addiction-related outcomes: craving, psychosocial adjustment and quality of life. Results: In accordance with impulsivity scores, the three groups of addicted individuals are best represented as two separate classes: Class 1 scored significantly higher than Class 2 in trait impulsivity measures, and performed worse than Class 2 in cognitive impulsivity tests. Class 1 was composed of significantly more cocaine users with comorbid personality disorders, whereas Class 2 was composed of significantly more pathological gamblers. As for outcome variables, individuals classified in Class 1 had higher levels of personality dysfunction and craving, and poorer psychosocial adjustment and quality of life. Conclusion: Our results support the value of impulsivity measures to identify clinically meaningful subgroups of addicted individuals that differ in diagnostic composition, dimensional estimates of personality pathology, and addiction-related outcomes.","palabras_clave":"Addiction|Personality and Personality Disorders|Psychosocial Functioning"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1498,"idioma_id":4,"titulo":"Dosage of Amyloid Precursor Protein Affects Axonal Contact Guidance in Down Syndrome.","resumen":"Amyloid precursor protein (APP), encoded on Hsa21, functions as a cell adhesion molecule (CAM) in axonal growth cones (GCs) of the developing brain. We show here that axonal GCs of human fetal Down syndrome (DS) neurons (and of a DS mouse model) overexpress APP protein relative to euploid controls. We investigated whether DS neurons generate an abnormal, APP-dependent GC phenotype in vitro. On laminin, which binds APP and beta1 integrins (Itgb1), DS neurons formed enlarged and faster-advancing GCs compared to controls. On peptide matrices that bind APP only, but not on those binding exclusively Itgb1 or L1CAM, DS GCs were significantly enlarged (2.0-fold), formed increased close adhesions (1.8-fold), and advanced faster (1.4-fold). In assays involving alternating stripes of monospecific matrices, human control GCs exhibited no preference for any of the substrates, whereas DS GCs preferred the APP-binding matrix (cross-over decreased significantly from 48.2 to 27.2%). Reducing APP expression in DS GCs with siRNA normalized most measures of the phenotype, including substrate choice. These experiments show that human DS neurons exhibit an APP-dependent, abnormal GC phenotype characterized by increased adhesion and altered contact guidance. The results suggest that APP overexpression may perturb axonal pathfinding and circuit formation in developing DS brain.","palabras_clave":"Brain Development|Neurodevelopmental Disorders"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1499,"idioma_id":5,"titulo":"Evolución del cultivo de garbanzo en la República Argentina.","resumen":"El garbanzo (Cicer arietinum L.) es una de las legumbres invernales más importantes en el mundo. Su cultivo se remonta a más de 7000 años en el oeste de Asia; incluso se han encontrado restos que comprueban su existencia desde hace 10.000 años en Turquía. El grano seco, utilizado para el consumo humano, es rico en proteínas: presenta valores desde el 22% al 28% de contenido proteico, además de poseer un 5% de grasa y un importante contenido de sales y minerales, entre ellos calcio, hierro y vitaminas. Al tradicional consumo del grano seco, se le suma el de la harina de garbanzo, típica de los países árabes y asiáticos y que goza de una creciente difusión. La superficie sembrada con garbanzo en la República Argentina entre los años 2001 al 2005 se mantuvo prácticamente estable, no superando las 2500 ha. A partir del año 2006 se produjo un pequeño incremento, alcanzando esta las 4000 ha y llegando a casi 5000 ha en el año 2007. En la campaña 2008, el área creció más del 50%, alcanzando en el año 2009 las 16.000 ha. Este crecimiento de la superficie cultivada con garbanzo se mantuvo: el área alcanzó las 40.000 ha en el año 2011, y las 125.000 ha en la campaña 2012 (es decir, aproximadamente el triple de la superficie del año anterior). En la provincia de Tucumán, el cultivo de esta legumbre invernal tuvo una evolución muy parecida; en el año 2004, se sembraron alrededor de 200 ha, pero esta superficie creció notablemente, llegando a las 28.080 ha en la campaña 2012. El crecimiento evidente de la superficie sembrada fue una consecuencia del creciente interés que sintieron los productores por el garbanzo, debido al precio que se llegó a pagar por su grano y por su necesidad de buscar nuevas alternativas al cultivo del trigo. Sus iniciativas encontraron el respaldo y apoyo de instituciones como el Instituto Nacional de Tecnología Agropecuaria (INTA) y la Estación Experimental Agroindustrial Obispo Colombres (EEAOC), que realizaron valiosos aportes al desarrollo de este cultivo. Es importante destacar que el garbanzo representa una alternativa válida para las zonas de regadío y las regiones subhúmedas-húmedas, como las pedemontanas del Noroeste Argentino (NOA). De hecho, estas son las zonas del país que reúnen las mejores condiciones agroecológicas para el cultivo, incluso comparadas con las zonas tradicionales de producción de estas legumbres invernales (centro y sur de la Argentina), considerando que su cosecha coincide con períodos lluviosos y de alta humedad atmosférica, que afectan la calidad del grano. La disponibilidad de variedades no es amplia, siendo Chañarito S-156 y Norteño las únicas inscriptas en nuestro país. Pero también existen otras poblaciones, entre las que se pueden mencionar Sauco, Mexicano y Blanco Lechoso. Además, en algunas zonas, pueden encontrarse otras variedades introducidas, tales como Blanco Sinaloa y Canadiense.","palabras_clave":"CICER ARIETINUM|ÁREA DE SIEMBRA|GARBANZO TIPO KABULI|GARBANZO TIPO DESI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1500,"idioma_id":5,"titulo":"TUC 403 y TUC 464: dos nuevas variedades de garbanzo tipo Kabuli para la región del Noroeste Argentino.","resumen":"El garbanzo (Cicer arietinum L.) es una de las legumbres más importantes cultivadas en el mundo, ocupando el segundo lugar después del cultivo de la soja. Su superficie ronda unos 11,5 millones de hectáreas y es la principal fuente de ingreso de los agricultores de escasos recursos en países como Etiopía, Tanzania y Kenia. En la India, es clave para la seguridad alimentaria, ya que este país es el primer productor, consumidor e importador de esta leguminosa. Desde el punto de vista genético, se reconocen dos tipos de garbanzo, el Kabuli y el Desi, cuyas diferencias más importantes radican en el tamaño de la semilla, la morfología de la planta, su rusticidad y comportamiento sanitario, entre otras. El tipo Kabuli presenta semillas de formas redondeadas y color crema, y un peso de 100 semillas mayor a 25 g, mientras que las 100 semillas del tipo Desi pesan menos de 25 g, tienen formas angulares y pueden ser de color verde, negro, amarillo, marrón o también crema. Los Kabuli presentan plantas de mayor altura (hasta 1 m) y los tallos, hojas y flores no contienen, en ningún caso, pigmentación antociánica; por el contrario, los Desi son plantas más bajas y generalmente presentan pigmentación antociánica. En la Argentina, se siembran solo cultivares de tipo Kabuli, que comprenden, a su vez, a los grupos saucos y mexicanos. Los primeros son de tamaño de semilla mediano (peso de 100 semillas que varía desde 40 g a 45 g), de color café con leche, y además se caracterizan por ser más resistentes al frío, a la sequía y a Fusarium que los mexicanos. Sin embargo, estos últimos son losque presentan una mejor calidad comercial, ya que sus semillas tienen un mayor tamaño que las de los saucos y son de color blanco, por lo que reciben un mayor precio en el mercado internacional. La disponibilidad de variedades en nuestro país no es amplia, siendo Chañarito S-156 y Norteño las únicas inscriptas. Pero también existen distintas poblaciones, entre las que se pueden mencionar el Sauco, de amplia adaptación pero con granos de baja calidad comercial, el Mexicano y el Blanco Lechoso, de excelente calidad comercial, pero muy sensibles a las heladas y susceptibles a la fusariosis. Otras variedades introducidas, tales como el Blanco Sinaloa -de muy buena calidad- y el Canadiense, se siembran en otras zonas del país. La Estación Experimental Agroindustrial Obispo Colombres (EEAOC) trabaja desde el año 2002 con la introducción y selección de germoplasma, procurando identificar genotipos de garbanzo que se adapten a la región del Noroeste Argentino (NOA) y que presenten buena calidad comercial. El presente trabajo tiene por objetivo describir el desarrollo de las nuevas variedades TUC 403 y TUC 464, así como también analizar su comportamiento agronómico y arquitectura de planta (adaptada a la trilla directa), los rendimientos y la calidad comercial de ambas.","palabras_clave":"CICER ARIETINUM|MEJORAMIENTO GENÉTICO|SELECCIÓN DE CULTIVARES|COMPORTAMIENTO AGRONÓMICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1501,"idioma_id":5,"titulo":"Efecto de la fecha de siembra en el comportamiento del cultivo del garbanzo en la provincia de Tucumán.","resumen":"El garbanzo representa un cultivo muy promisorio en el Noroeste Argentino (NOA), pues fundamentalmente en los últimos años, ha demostrado que es una alternativa de siembra invernal más que interesante, junto con el trigo. El destino principal de la producción es la exportación, mientras que un porcentaje muy bajo es destinado al consumo interno, principalmente como grano seco, pero también como harina para la elaboración de otros alimentos, tales como el fainá y las hamburguesas. La siembra de este cultivo se realiza desde el mes de abril hasta mediados de junio, dependiendo esta decisión de muchos factores, entre los cuales pueden citarse los siguientes: la humedad disponible en el perfil del suelo, la ocurrencia de heladas en los períodos críticos de la planta, la fecha de cosecha del cultivo antecesor y la probabilidad de lluvias en época de cosecha (factor que deteriora la calidad del grano). En los esquemas productivos de la época estival en la provincia de Tucumán, los cultivos más generalizados son la soja, el maíz y ocasionalmente el poroto. En términos generales, el garbanzo es el cultivo que le sigue al de soja, ya que este último desocupa el lote en fechas de siembra recomendadas para el garbanzo. Ocasionalmente, puede también sembrarse después del poroto, cuando este cultivo se realiza en zonas que no son tan marginales y donde existe la posibilidad de realizar riego. La fecha de siembra más generalizada para el garbanzo en Tucumán corresponde a la primera quincena de mayo, ya que generalmente alrededor de esa fecha la cosecha de soja de grupos de madurez corto ya ha sido efectuada. Por otro lado, en años normales las lluvias ocurridas hasta ese periodo permiten recargar la humedad del perfil del suelo. Las siembras tempranas, correspondientes al mes de abril, no son muy frecuentes, a pesar de que es generalmente en este momento en que se registra una humedad óptima en el perfil del suelo. Esto se debe, por un lado, a que los lotes están aún ocupados por la soja, y por otro, a que son mayores las probabilidades de que el cultivo sufra de heladas en los periodos críticos, tales como la floración y el cuaje de granos. En algunas localidades de la zona sub-húmeda seca, se dejan los lotes desocupados durante el verano, como barbecho, para favorecer la acumulación de humedad y así permitir que se realicen siembras tempranas. Con el objetivo de poder dilucidar cuál es la fecha óptima de siembra del cultivo del garbanzo en la provincia de Tucumán, el Proyecto Legumbres Secas de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC) ha llevado a cabo ensayos con distintas fechas de siembra, cuyos resultados se dan a conocer en el presente trabajo.","palabras_clave":"CICER ARIETINUM|PRÁCTICAS CULTURALES|NOROESTE ARGENTINO|COMPORTAMIENTO DE CULTIVARES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1503,"idioma_id":5,"titulo":"Concentración de pesticidas organoclorados en aire del sur de la cuenca del Plata: un año de muestreo","resumen":"Con el objeto de estudiar la concentracióny distribución de plaguicidas organoclorados(POCLs) en aire del sur de la cuenca del Plata seinstalaron muestreadores pasivos de espuma depoliuretano (n=21) recolectados cada 3-5 mesesdurante un año.La concentración media anual de POCLs oscilóentre 326 y 9000 pg.m-3con predominancia deendosulfanos (Endo) (40-98 % del total). Lospromedios anuales de Endo (230-8936 pg.m-3)son superiores a los reportados para otros paísesde la región (Brasil, Bolivia, Chile y Colombia:114-898 pg.m-3). Los valores máximos alcanzadoscorresponden al período cálido, en zonas demayor actividad sojera: Norte de Buenos Airesy Sur de Entre Ríos y Santa Fe (3072-21521pg.m-3).Las concentraciones de DDTs (12-235 pg.m-3)resultan un orden de magnitud inferiores a lasde sitios en los que actualmente se utiliza paracombatir la malaria (India: 5356 pg.m-3; Surde México: 2360 pg.m-3) pero la relación DDT\/DDE (1.1-6.8) > 1 encontrada sugiere un aporterelativamente fresco. Esto podría deberse al usode Dicofol en la región, que presenta residuos deeste compuesto.Los HCHs presentaron una alta concentraciónen Zárate (801-8348 pg.m-3) donde resultaronpredominantes (66 % del total) con una relación&#945;\/&#947;HCHs &#8776;7, similar a la de la mezcla técnicaantiguamente usada, mientras que las demásestaciones tuvieron concentraciones un ordende magnitud más bajas (7-199 pg.m-3) pero deaportes más recientes.En la zona metropolitana se destacaron losclordanos (83-691 pg.m-3) que representan 5-45% del total, especialmente en la Capital Federal,donde la relación trans\/cis-clordano>1.6 indicaun aporte fresco.Se concluye que los POCLs presentandiferencias en su distribución espacial; altaconcentración de endosulfanos en zonaagrícola; heterogeneidad de pesticidas y mayorconcentración de clordanos en zonas urbanas.Los valores de las concentraciones y de relacionesentre compuestos sugieren aportes frescosclandestinos, o transporte a gran escala.","palabras_clave":"pesticidas organoclorados|aire|cuenca del plata"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1504,"idioma_id":5,"titulo":"ALFABETIZACIÓN ACADÉMICA Y EMOCIONAL","resumen":"En este sentido, el presente trabajo apunta a potenciar algunos aspectos que consideramos condicionantes del aprendizaje académico, emocional y creativo: patrones de aprendizaje, inteligencia emocional y creatividad, para lograr conceptualizaciones que permitan constituir avances en el campo de la psicología educacional y realizar contribuciones prácticas: orientaciones a docentes y alumnos con el objetivo de promover procesos educativos que atiendan a diversas formas de acceder y construir conocimientos, y estimulen el inicio y la continuidad en las carreras de grado en pos de lograr la integración y permanencia en sus estudios. El escrito se organiza en tres apartados. El primero presenta las consideraciones teóricas respecto a las dimensiones del proceso de enseñanza-aprendizajes que se estudian: patrones de aprendizaje, creatividad e inteligencia emocional. El segundo se divide en dos secciones, una que muestra los principales resultados encontrados en torno a las variables en estudio y otra que da cuenta de las principales contribuciones acerca de los talleres llevados a cabo con alumnos y docentes. En el último, se exponen las consideraciones finales y futuras líneas a seguir investigando y propuestas practicas a partir de los resultados encontrados.","palabras_clave":"Alfabetización académica|Emociones|Creatividad|Universidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1505,"idioma_id":5,"titulo":"La Sociedad Rural Argentina y el golpe de estado de 1966: el accionar político de una corporación económica.","resumen":"El golpe de estado de 1966 contra Arturo Illia fue llevado a cabo por las Fuerzas Armadas. Sin embargo, este artículo desarrolla la hipótesis de que la Sociedad Rural (SRA) contribuyó a crear en el período anterior un clima destituyente a través de una serie de canales de participación directos e indirectos, transformándose en promotora del golpe de estado y por lo tanto constituyendo un eje fundamental de legitimación de la interrupción del orden político. La vinculación de sus propuestas y de las políticas efectivamente aplicadas a partir de la dictadura militar de Juan Carlos Onganía es un dato significativo que da cuenta de lo que constituye un entramado de complicidad cívico-militar que posibilitó y facilitó el derrocamiento del presidente. A pesar de este gran apoyo inicial, el agroexportador no sería el sector especialmente beneficiado por su política. Para desplegar la hipótesis y analizar las distintas variables que ello implica se utilizará tanto una completa y variada bibliografía sobre el tema, como fuentes primarias locales que incluyen las publicaciones oficiales de la corporación (Memorias de la SRA) como las declaraciones de sus máximos exponentes realizadas a través de la prensa.","palabras_clave":"SOCIEDAD RURAL ARGENTINA|GOLPE DE ESTADO 1966|ILLIA|CORPORACIONES ECONÓMICAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1507,"idioma_id":4,"titulo":"Microleakage of a self- adhesive flowable resin as pit and fissure sealant.","resumen":"IDEM AL FULL TEXT","palabras_clave":"SELF ADHESIVE RESIN|SEALANTS|MICROLEAKAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1508,"idioma_id":4,"titulo":"Towards Teaching Artificial Intelligence Using a Model Driven Approach","resumen":"In computer science, it is quite difficult to teach well an introductory course on AI, partly because AI lacks a unified methodology, overlaps with many other disciplines, and involves a wide range of skills from very applied to quite formal. When teaching Artificial Intelligence, models are the principal artifacts used by professors to communicate concepts. These models should be used as part of a technical answer of a practical work, with the aim of narrowing the distance between concepts and their implementation in a programming language. The model-driven development presents a very promising approach to reduce the difficulties in the generation of code solutions. This work presents an MDD-based method, a language to define intelligent agent and a computational tool that gives support to the MDD-based method.","palabras_clave":"artificial intelligence|education|intelligent agents|model-driven development"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1509,"idioma_id":5,"titulo":"ANÁLISIS CRÍTICO DE LAS METODOLOGÍAS DE VALUACIÓN DE PROYECTOS DE INVESTIGACIÓN Y DESARROLLO DE SEMILLAS","resumen":"En el presente trabajo se estudiará la problemática de evaluar proyectos de investigación y desarrollo (I+D) de semillas utilizando la metodología de opciones reales. En particular se discute la relevancia del modelo propuesto por Jason Hsu y Eduardo Schwartz para el ámbito farmacéutico.   El interés por este tema surge luego de observar que los proyectos en el sector de innovación agropecuaria son analizados como flujos de fondos ciertos, sin tener en cuenta la probabilidad de llegar al resultado esperado o no, y en qué momento se llega a este resultado o se decide desechar la investigación. El resultado de este punto de vista es la selección de proyectos con alta rentabilidad pero muy riesgosos, por sobre otros con probabilidades de éxito superiores.Este tipo de inversiones tiene tres características que no permiten utilizar técnicas tradicionales de valuación. En primer lugar, las inversiones son en su mayoría irreversibles y sus costos de capital hundidos. En segundo lugar, existe incertidumbre sobre el futuro retorno de la inversión (ya que los precios futuros son impredecibles). En último lugar, los inversores muchas veces tienen la opción de esperar para obtener una mejor información sobre el precio futuro. Por otro lado, estos proyectos requieren varios años para producir el bien comercializable, siendo necesario contar con etapas de desarrollo, testeo y registración en entes de regulación. Adicionalmente, los costos son sumamente altos, siendo aproximadamente el 25% exclusivo de la etapa de registración y aprobación en entes públicos . El desarrollo de un solo evento de semilla (por ejemplo tolerancia a un determinado agroquímico o insectos) se extiende en promedio 13 años (ya que varias de estas etapas se realizan en simultáneo) y tiene un costo de USD 136.000.000.  Este trabajo consta de tres secciones. En la primera se plantean los métodos tradicionales de valuación y se introduce la valuación por opciones reales. En la segunda sección se realiza una introducción y explicación de los elementos claves del modelo propuesto por Hsu y Schwartz. Por último se resumen los resultados obtenidos y se concluye.","palabras_clave":"Semillas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1510,"idioma_id":4,"titulo":"?\t?Hypothyroidism and Oxidative Stress: Differential Effect on the Heart of Virgin and Pregnant Rats.?","resumen":"The present study investigates the effects of hypothyroidism on both the redox state and the thyroid hormone receptors expression in the heart ventricle of virgin and pregnant rats.Hypothyroid state was induced by 6-n-propyl-2-thiouracil in drinking water given to Wistar rats starting 8 days before mating until day 21 of pregnancy or for 30 days in virgin rats. Serum paraoxonase-1 (PON-1) activity, serum and heart nitrites, and thiobarbituric acid-reactive substances (TBARS) were analyzed. Heart protein oxidation, as carbonyls, and copper-zinc superoxide dismutase (CuZnSOD), glutathione peroxidase (GPx), and catalase (CAT) activities, were determined. In addition, heart expressions of NADPH oxidase (NOX-2), CAT, SOD, GPx, and thyroid receptors (TRá and TRâ) mRNA were assessed by RT-PCR. Inducible and endothelial Nitric Oxide Synthase (iNOS and eNOS) were determined by Western blot. Hypothyroidism in the heart of virgin rats decreased TRá and TRâ expressions, and induced oxidative stress, leading to a decrease of nitrites and an increase of carbonyls, NOX-2 mRNA, and GPx activity. A decreased PON-1 activity suggested low protection against oxidative stress in blood circulation. Pregnancy reduced TRá and TRâ mRNA expressions and induced oxidative stress by increasing nitrite and TBARS levels, SOD and CAT activities and NOX-2, eNOS and iNOS expressions, while hypothyroidism, emphasized the decreases of TRá mRNA levels and did not alter the redox state in the heart. TR expressions and redox balance of rat hearts depend on the physiological state. Pregnancy per se seems to protect the heart against oxidative stress induced by hypothyroidism","palabras_clave":"thyroid hormone|thyroid hormone receptor|redox state|myocardium"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1511,"idioma_id":4,"titulo":"Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation","resumen":"Steroid receptors were classically described regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic Progesterone Receptor binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CBP and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI\/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly specifically in the proximity of the intragenic PRbs.  PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) in its active elongating form. Together these events promoted the re-distribution of the active Pol II toward the 3´end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which  PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes.","palabras_clave":"BCL-X|PROGESTERONE RECEPTOR|ELONGATION|INTRAGENIC SITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1513,"idioma_id":5,"titulo":"Estados Generales del Tercer Cine. Los Documentos de Montreal","resumen":"Este Cuaderno de la Red de Historia de los Medios (Rehime) presenta documentos inéditos, hasta ahora inaccesibles, a pesar de su relevancia para el cine político mundial de las décadas de 1960 y 1970. Se trata de las conferencias, talleres y debates que tuvieron lugar entre el 2 y el 8 de junio de 1974 en Montreal, durante los Rencontres Internationales pour un Nouveau Cinéma. Se trató del encuentro del cine político mundial más importante de ese período (década del 60 y 70). Allí confluyeron destacados realizadores, productores y grupos de cine del 68 en el Primer Mundo, del cine político latinoamericano, y de las nacientes cinematografías africanas.  El título propuesto para el artículo y el Cuaderno en su conjunto, Estados Generales del Tercer Cine, intenta poner de relieve la urgencia revolucionaria presente en los proyectos de ese período.  El registro audiovisual del encuentro (inédito), del cual pueden verse fragmentos significativos en el DVD que acompaña el Cuaderno, permite reconstruir en forma vívida los debates de una coyuntura política intensa, protagonizados por latinoamericanos como Miguel Littín, Julio García Espinosa, Fernando Solanas o Walter Achugar; africanos como Med Hondo, Tahar Cheriaa, Férid Boughedir o Lamine Merbah; críticos europeos como Guido Aristarco, Guy Hennebelle, Lino Micciché o Jean Partick Lebel; quebequenses como Fernand Dansereau, Gilles Groulx, André Paquet, Yvan Patry; así como distribuidoras y grupos del cine paralelo como Slon\/Iskra, Tricontinental, Mk2, The Other Cinema, entre muchos otros.    El editorial y el artículo que se adjuntan (a cargo de M.Mestman) analizan esa historia y el aporte de estos nuevos documentos para una revisión o reescritura de la historia cine político del período, en particular, en este caso, sobre la influencia tercermunidsta en el llamado Nuevo Cine Latinoamericano. Junto al artículo el Cuaderno en su conjunto contiene los documentos del Encuentro de Montreal y las discusiones allí sucitadas, del mismo modo que estas se compilan en un DVD de más de 2 horas que acompaña el Cuaderno.","palabras_clave":"TERCER CINE|DOCUMENTOS AUDIOVISUALES|ARCHIVO|ESTUDIOS TRANSNACIONALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1514,"idioma_id":5,"titulo":"ESTRATEGIAS Y MOTIVACIONES DE CAPITALIZACIÓN ENTRE CONTRATISTAS DE MAQUINARIA AGRÍCOLA PAMPEANA","resumen":"Resumen  Los contratistas de maquinaria agrícola en la Región Pampeana han cobrado una importancia manifiesta, a punto tal de no poder entenderse la producción agrícola contemporánea sin su presencia. Basta decir que en 2012 entre el 70% y el 85% de las superficies implantadas con los 5 principales cultivos, 31.000.000 ha, estuvieron en manos de estos sujetos. (Peretti, 2013: 37)  Un equipo de siembra, competitivo en el mercado de servicios, cuesta entre U$s 120.000 y U$s 500.000; uno de cosecha entre U$s. 200.000 y U$s 800.000 y no puede tener más de 5 años de antigüedad. Sostener este nivel de capitalización y seguir acumulando dependen de varios factores, pero la mano de obra, la relación con la toma de créditos y el perfil de los demandantes se han revelado como decisivos para comprender el funcionamiento de estas empresas. En el presente artículo nos ocuparemos de describir y analizar el modo en que estas dimensiones intervienen en las decisiones de estos sujetos y qué tipo de lógicas se ponen en juego al momento de tomar cada una de las decisiones.","palabras_clave":"Contratistas de maquinaria Agrícola|Mano de obra|Toma de Crédito|Región Pampeana"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1515,"idioma_id":4,"titulo":"Ecological, Development and Capital Contradictions in Latin America","resumen":"This article is about the social and ecological crisis in Latin America which is closely related to the social and economic development process. The underdeveloped economy has determined the general features of the rural and urban regions. Poverty, social exclusion and environmental conflicts are some of the general consequences of the capital contradictions which define an underdeveloped process of growth.","palabras_clave":"Ecologia|Crisis|Am Latina|Desarrollo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1517,"idioma_id":4,"titulo":"Limited Packing and Multiple Domination problems: polynomial time reductions","resumen":"The Limited Packing and Multiple Domination problems in graphs have closely-related definitionsand the same computational complexity on several graph classes. In this work wepresent two polynomial time reductions between them. Besides,wetake into considerationgeneralized versions of these problems and obtain polynomial time reductions betweeneach one and its generalized version.","palabras_clave":"K-TUPLE DOMINATING SET|K-LIMITED PACKING|COMPUTATIONAL COMPLEXITY|POLYNOMIAL TIME REDUCTIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1522,"idioma_id":4,"titulo":"The thing in-itself and its role in the constitution of objectivity. A critical reading of Onof´s reconstruction of transcendental affection","resumen":"In this paper I consider some conclusions drawn by Christian Onof in a recent paper, in which the author analyzes the problem of transcendental affection. Onof assumes that Kant´s references to the thing-in-itself as the cause (or ground) of sensible affection reveal a commitment to a metaphysical assumption, that is: the existence of something real beyond the subject's mind. I will suggest, on the contrary, that Kant´s confidence in the existence of a non-subjective reality should not be characterized as a metaphysical but as a pre-philosophical assumption, that is: an unproblematic starting point for the critical investigation which Kant did not consider as demanding any particular justification. In the second place, I will try to show that affection should not be characterized (as Onof suggests) as a transcendental condition of the constitution of objectivity, but rather as a transcendent condition (directly related to sensations, which provide the matter of appearances). Finally, I will try to demonstrate that the two-aspects interpretation (which Onof explicitly rejects) is compatible with the conception of the thing-in-itself as the unknown cause or ground of appearances, since it is possible to articulate such conception with the thesis that affirms that the thing-in-itself is nothing ontologically different from the empirical object given in experience.","palabras_clave":"THING-IN-ITSELF|OBJECTIVITY|TRANSCENDENTAL AFFECTION|TRANSCENDENTAL CONDITIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1530,"idioma_id":4,"titulo":"Robust model predictive control of a Wiener-like system","resumen":"In this paper, a robust model predictive control for a Wiener-like system is presented. The proposed system consists of a lineal dynamic block  represented by Laguerre or Kautz basis followed by a High Level Piecewise Linear function. The results are evaluated on the basis of a simulation of a distillation column.","palabras_clave":"HLCPWL functions|Wiener|Predictive control"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1531,"idioma_id":4,"titulo":"Assessment of remotely-sensed sea-surface temperature and chlorophyll- a concentration in San Matías Gulf (Patagonia, Argentina)","resumen":"Field measurements of surface temperature and chlorophyll-a concentration were used to evaluate for the first time the performance of the standard seasurface temperature and chlorophyll-a algorithms of the Moderate Resolution Imaging Spectroradiometer (MODIS) in San Matías Gulf, northern of the Argentine Patagonian Continental Shelf (between40º47'S and 42º13'S). The fit of the temperature data to a Standard Major Axis (SMA) typeII regression model indicated that a high proportion of the total variance (r2&gt;0.80) was explained by the model. For chlorophyll-a, the low correlation (r2=0.15) andhigh dispersion indicated a poor performance and a general overestimation of Chl-a by the OC3Mv5 algorithm as indicated by the Relative Percent Difference (RPD=113%). However, for stations located in the northern area of the gulf the result showed a higher correlation (r2=0.76) and less dispersion (RPD=31%). The feasible reasons and explanations for the performance of NLSST and OC3Mv5 algorithms are discussed. The results of the temporal variability analysis of SST and Chl-a in different areas of the gulf agreed with previous studies.","palabras_clave":"temperature|chlorophyll-a|in-situ|MODIS|San Matías Gulf"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1532,"idioma_id":4,"titulo":"Seasonal succession of zooplankton in coastal waters of the Argentine Sea (Southwest Atlantic Ocean): prevalence of classical or microbial food webs","resumen":"The demographic characteristics of marine zooplankton make it especially suitable for examining the variability of marine ecosystems. The zooplankton annual succession was studied at a permanent coastal station in the Argentine Sea (38°28&#8242;S, 57°41&#8242;W) in relation to physical conditions and phytoplankton size fractions. Small copepods (<1 mm total length), mainly represented by Oithona nana (Cyclopoida) and adults and copepodites of Calanoida, numerically dominated the metazooplankton throughout the year. In summer, small copepods also exceeded large copepods in biomass. Larvaceans (mostly <1 mm total length) were the second most important metazooplankton group, with strong dominance of Oikopleura dioica. The zooplankton succession exhibited two main periods throughout the year: (1) a cold winter\u2013spring period characterized by a dominant classical herbivore food web in which the large copepod Calanoides carinatus and lamellibranch larvae were associated with the lowest temperatures and highest Chl-a and microphytoplankton, and (2) a warm summer period dominated by a microbial food web in which microbial filter-feeders such as Oithona nana, Paracalanus spp., Oikopleura dioica and Penilia avirostris predominated and the highest density of picophytoplankton and lowest concentrations of Chl-a were recorded. The implications of the present findings for the growth and survival of fish larvae distributed in the study area are discussed.","palabras_clave":"MESOZOOPLANKTON SUCCESSION|SMALL COPEPODS|LARVACEANS|PHYTOPLANKTON FRACTIONS|EPEA STATION|ARGENTINE SEA|SW ATLANTIC OCEAN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1534,"idioma_id":4,"titulo":"Therapeutic efficacy of melatonin in reducing retinal damage in an experimental model of early type 2 diabetes in rats","resumen":"Diabetic   retinopathy (DR) is a leading cause of acquired blindness in adults,   mostly affected by type 2 diabetes mellitus (T2DM). We have developed an   experimental model of early T2DM in adult rats which mimics some   features of human T2DM at its initial stages and provokes significant   retinal alterations. The aim of this work was to analyze the effect of   melatonin on retinal changes induced by the moderate metabolic   derangement. For this purpose, adult male Wistar rats received a control   diet or 30% sucrose in the drinking water. Three weeks after this   treatment, animals were injected with vehicle or streptozotocin (STZ,   25 mg\/kg). One day or 3 wk after vehicle or STZ injection, animals were   subcutaneously implanted with a pellet of melatonin. Fasting and   postprandial glycemia, and glucose, and insulin tolerance tests were   analyzed. At 12 wk of treatment, animals which received a   sucrose-enriched diet and STZ showed significant differences in   metabolic tests, as compared with control groups. Melatonin, which did   not affect glucose metabolism in control or diabetic rats, prevented the   decrease in the electroretinogram a-wave, b-wave, and oscillatory   potential amplitude, and the increase in retinal lipid peroxidation, NOS   activity, TNF&#945;, Müller cells glial fibrillary acidic protein, and   vascular endothelial growth factor levels. In addition, melatonin   prevented the decrease in retinal catalase activity. These results   indicate that melatonin protected the retina from the alterations   observed in an experimental model of DR associated with type 2 diabetes.","palabras_clave":"DIABETIC RETINOPATHY|EXPERIMENTAL TYPE 2 DIABETES|MELATONIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1536,"idioma_id":4,"titulo":"Prenatal maternal restraint stress exposure alters the reproductive hormone  profile and  testis development of therat male offspring.","resumen":"Severalstudies have demonstrated that the presence of stressors during pregnancyinduces adverse effects on the neuroendocrine system of the offspring later inlife. In the present work, we investigated the effects of early programming onthe male reproductive system, employing a prenatal stress (PS) paradigm. Thisstudy found that when pregnant dams were placed in a plastic restrainer threetimes a day during the last week of pregnancy, the offspring showed reducedanogenital distance and delayed testicular descent. Serum luteinising hormone(LH) and follicle-stimulating hormone (FSH) levels were decreased at postnatalday (PND) 28 and testosterone was decreased at PND 75. Increased testosteroneplus dihydrotestosterone (T+DHT) concentrations correlated with increased testicular 5alpha- Reductase-1 (5alpha-R-1) mRNA expression at PND 28.Moreover, PS accelerated spermatogenesis at PND 35 and 60, and increased meanseminiferous tubule diameter in pubertal offspring and reduced Leydig cellnumber was observed at PND 35 and 60. PS offspring had increased androgenreceptor (AR) mRNA level at PND 28, and at PND 35 had increased the numbers ofSertoli cells immunopositive for AR. Overall, the results confirm that stressduring gestation can induce long-term effects on the male offspringreproductive system. Of particular interest is the pre-pubertal imbalance ofcirculating hormones that probably trigger accelerated testicular development,followed by an increase in total androgens and a decrease in testosteroneconcentration during adulthood. Exposure to an unfavourable intrauterineenvironment might prepare for harsh external conditions by triggering earlypuberty, increasing reproductive potential.","palabras_clave":"PITUITARY HORMONES|STRESS PRENATAL|RAT|SPERMATOGENESIS|STEROID HORMONES|TESTIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1537,"idioma_id":4,"titulo":"Increasing capacity for environmental engineering in Salta, Argentina","resumen":"Background: The Fogarty International Centers of the United States National Institutes of Health includes the International Training and Research in Environmental and Occupational Health (ITREOH) Program.   Results: The International Training Program in Environmental Toxicology and Public Health Center, part of the ITREOH group of Centers, is based at the University of California, Davis and has major efforts centered at the public universities in Montevideo, Uruguay and Salta, Argentina. This Center was first funded in the second cycle of ITREOH grants, in 2002. Initial efforts were focused in Uruguay; significant activities in Salta were only begun in 2005. Training and research efforts in Salta are centered in the College of Engineering, which is unconventional for NIH funding in public health.   Conclusions: This paper describes the philosophical basis for integration of training in engineering with environmental toxicology, microbiology, and public health, historical and current accomplishments of this program, and its future goals.","palabras_clave":"FOGARTY CENTER|ITREOH PROGRAM|INFECTIOUS DISEASE|WATER QUALITY|DISTANCE LEARNING|TRAINING GRANT|GRADUATE EDUCATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1539,"idioma_id":4,"titulo":"Search for supersymmetry in events with photons, bottom quarks, and missing transverse momentum in proton-proton collisions at a centre-of-mass energy of 7 TeV with the ATLAS detector","resumen":"A search has been performed for the experimental signature of an isolated photon with high transverse momentum, at least one jet identified as originating from a bottom quark, and high missing transverse momentum. Such a final state may originate from supersymmetric models with gauge-mediated supersymmetry breaking in events in which one of a pair of higgsino-like neutralinos decays into a photon and a gravitino while the other decays into a Higgs boson and a gravitino. The search is performed using the full dataset of 7 TeV proton-proton collisions recorded with the ATLAS detector at the LHC in 2011, corresponding to an integrated luminosity of 4.7 fb^-^1. A total of 7 candidate events are observed while 7.5+\/-2.2 events are expected from the Standard Model background. The results of the search are interpreted in the context of general gauge mediation to exclude certain regions of a benchmark plane for higgsino-like neutralino production.A search has been performed for the experimental signature of an isolated photon with high transverse momentum, at least one jet identified as originating from a bottom quark, and high missing transverse momentum. Such a final state may originate from supersymmetric models with gauge-mediated supersymmetry breaking in events in which one of a pair of higgsino-like neutralinos decays into a photon and a gravitino while the other decays into a Higgs boson and a gravitino. The search is performed using the full dataset of 7 TeV proton-proton collisions recorded with the ATLAS detector at the LHC in 2011, corresponding to an integrated luminosity of 4.7 fb-1. A total of 7 candidate events are observed while 7.5 pm 2.2 events are expected from the Standard Model background. The results of the search are interpreted in the context of general gauge mediation to exclude certain regions of a benchmark plane for higgsino-like neutralino production.","palabras_clave":"ATLAS|high|energy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1545,"idioma_id":4,"titulo":"Precocious sexual signalling and mating in Anastrepha fraterculus (Diptera: Tephritidae)  sterile males achieved through juvenile hormone treatment and protein supplements.","resumen":"Sexual maturation of Anastrepha fraterculus is a long process. Methoprene (a mimic of juvenile hormone) considerably reduces the time for sexual maturation in males. However, in other Anastrepha species, this effect depends on protein intake at the adult stage. Here, we evaluated the mating competitiveness of sterile laboratory males and females that were treated with methoprene (either the pupal or adult stage) and were kept under different regimes of adult food, which varied in the protein source and the sugar:protein ratio. Experiments were carried out under semi-natural conditions, where laboratory flies competed over copulations with sexually mature wild flies. Sterile, methoprene-treated males that reached sexual maturity earlier (six days old), displayed the same lekking behaviour, attractiveness to females and mating competitiveness as mature wild males. This effect depended on protein intake. Diets containing sugar and hydrolyzed yeast allowed sterile males to compete with wild males (even at a low concentration of protein), while brewer´s yeast failed to do so even at a higher concentration. Sugar only fed males were unable to achieve significant numbers of copulations. Methoprene did not increase the readiness to mate of six-day-old sterile females. Long pre-copulatory periods create an additional cost to the management of fruit fly pests through the sterile insect technique (SIT). Our findings suggest that methoprene treatment will increase SIT effectiveness against A. fraterculus when coupled with a diet fortified with protein. Additionally, methoprene acts as a physiological sexing method, allowing the release of mature males and immature females and hence increasing SIT efficiency.","palabras_clave":"STERILE INSECT TECHNIQUE|SEXUAL MATURATION|MATING COMPETITIVENESS|LEKKING BEHAVIOUR|JUVENILE HORMONE|METHOPRENE|NUTRITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1547,"idioma_id":4,"titulo":"Physiologic Versatility and Growth Flexibility as the Main Characteristics of a Novel Thermoacidophilic Acidianus Strain Isolated from Copahue Geothermal Area in Argentina","resumen":"A novel species  of archaea has been isolated in pure culture from various geothermal  acidic hot springs in Copahue, Argentina. One of the most striking  characteristic of this isolate, that we have called ALE1, is its  amazing metabolic versatility. It has proven able to grow on sulphur,  tetrathionate, iron (II) and sucrose under aerobic conditions, but it  can also develop under anaerobic conditions using ferric iron or  sulphur as electron acceptors and sulphur or hydrogen as electron  donors autotrophically. Temperature of 75 ºC and pH between 2.5 and  3.0 are ALE1 strain optimal growth conditions, however cells are able  to oxidise iron (II) even at pH 1.0. This thermoacidophilic archaeon has an irregularly coccus-shaped form  surrounded by a regularly arrayed glycoprotein layer (S-layer).  Phylogenetic  analysis shows that ALE1 strain belongs to Sulfolobaceae family in the Thermoprotei class, within the Crenarchaeota phylum. Based on 16S rRNA gene sequence similarity on NCBI data base,  ALE1 does not have very close related sequences reported, cultured  or, which is more surprising, uncultured. Its closest related species  are strains of Acidianus  hospitalis (91 % of sequence similarity), Acidianus  infernus (90 %), Acidianus  ambivalens (90 %) and Acidianus  manzanensis (90 %). Its DNA base composition of 34.5% mol C+G is higher than  reported for other Acidianus species. Considering all these extraordinary physiological and  phylogenetic characteristics of ALE1 strain, we considered it to  represent a novel species of the genus Acidianus and we propose to name it Acidianus  copahuensis. The aim of this study is to characterise physiologically this novel  archaeal isolation in order to understand its role in iron and  sulphur geochemical cycles in Copahue geothermal area and to evaluate  its potential applications in diverse bioprocesses, in particular,  bioleaching and biooxidation.","palabras_clave":"Acidianus|extremophiles|Copahue|thermoacidophilic archaea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1556,"idioma_id":4,"titulo":"Antiadipogenic effect of carnosic acid, a natural compound present in Rosmarinus officinalis, is exerted through the C\/EBPs and PPAR&#227; pathways at the onset of the differentiation program","resumen":"    Background: Obesity is a serious health  problem all over the world, and inhibition of adipogenesis constitutes one of  the therapeutic strategies for its treatment. Carnosic  acid (CA), the  main bioactive compound of Rosmarinus  officinalis extract, inhibits 3T3-L1 preadipocytes differentiation. However,  very little is known about the molecular mechanism responsible for its  antiadipogenic effect.     Methods: we evaluated the effect of  CA on the differentiation of 3T3-L1 preadipocytes analyzing the process of  mitotic clonal expansion, the level of adipogenic markers, and the subcelular  distribution of C\/EBP&#946;.    Results: CA treatment only during the  first day of 3T3-L1 differentiation process was enough to inhibit adipogenesis.  This inhibition was accompanied by a blockade of mitotic clonal expansion. CA did  not interfere with C\/EBP&#946; and C\/EBP&#948; mRNA levels but blocked PPAR&#947;, and FABP4 expression.  C\/EBP&#946; has different forms known as LIP and LAP. CA induced an increase in the  level of LIP within 24 h of differentiation, leading to an increment in LIP\/LAP  ratio. Importantly, overexpression of LAP restored the capacity of 3T3-L1  preadipocytes to differentiate in the presence of CA. Finally, CA promoted  subnuclear de-localization of C\/EBP&#946;.     Conclusions: CA exerts its anti-adipogenic  effect in a multifactorial manner by interfering mitotic clonal expansion,  altering the ratio of the different C\/EBP&#946; forms, inducing the loss of C\/EBP&#946;  proper subnuclear distribution, and blocking the expression of C\/EBP&#945; and PPAR&#947;.  General  significance:  understanding the molecular mechanism by which CA blocks adipogenesis is  relevant because CA could be new a food additive beneficial for the prevention  and\/or treatment of obesity.   ","palabras_clave":"ADIPOGENESIS|CARNOSIC ACID|C\/EBPs|OBESITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1563,"idioma_id":4,"titulo":"Vision-Based Interfaces Applied to Assistive Robots","resumen":"This paper presents two vision&#8208;based interfaces  for disabled people to command a mobile robot for  personal assistance. The developed interfaces can be  subdivided according to the algorithm of image  processing implemented for the detection and tracking of  two different body regions. The first interface detects and  tracks movements of the user\u2019s head, and these  movements are transformed into linear and angular  velocities in order to command a mobile robot. The  second interface detects and tracks movements of the  user\u2019s hand, and these movements are similarly  transformed. In addition, this paper also presents the  control laws for the robot. The experimental results  demonstrate good performance and balance between  complexity and feasibility for real&#8208;time applications.","palabras_clave":"Vision&#8208;Based Interface|Assistance Robot|Non&#8208;Linear Control"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1566,"idioma_id":4,"titulo":"Surface modification of titanium by anodic oxidation in phosphoric acid at low potentials. Part 1. Structure, electronic properties and thickness of the anodic films","resumen":"Titanium surface characteristics determine the degree of success of permanent implants. The topography, morphology of the surface in micro and nano scales, the impurities present and other characteristics are a main concern, and therefore a multi-technique approach is required in order to evaluate modification process effects on the surface. Surface modification of titanium in the nanometrical range was performed by means of anodisation in phosphoric with the aim of improving both the biocompatibility and the corrosion resistance in the biological media.&nbsp; Biocompatible characteristics of the modified titanium surface, as the presence of anatase in the oxide film and the incorporation of phosphate to the surface, were determined. Moreover, the electronic properties of the surface oxide presented a carrier number adequate for biomedical applications. The increase in the film thickness from 3 to 42 nm was estimated from EIS results when anodising potentials from 0 to 30 V were applied, whereas a bi-layer structure of the protective oxides formed was determined","palabras_clave":"TITANIUM|ANODIC OXIDE|BIOMATERIALS|SURFACE MODIFICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1569,"idioma_id":4,"titulo":"Nutritional and phytochemical study of ilex paraguariensis fruits","resumen":"&nbsp;Yerba mate is defined as the product constituted by the dried, slightly roasted and milled leaves of Ilex paraguariensis. However, the fruits of this species are often found in the commercial product. Nowadays the fruits are considered a by-product. The objective of this work was to obtain the preliminary data of minerals, lipids, methylxanthines and polyphenols in the ripe fruits of I. paraguariensis. The results showed a considerable amount of total dietary fiber (42.0±1.6 g\/100 g) and nutritionally valuable minerals: potassium (1324±15 mg\/100g), iron (6.4 ± 0.5mg\/100g), magnesium (168±15 mg\/100g), calcium (150±12 mg\/100g), copper (1.1±0.1mg\/100g), zinc (2.3±0.3mg\/100g) and sodium (1.3±0.1 mg\/100g).The lipids content was 4.5 g\/100g. Oleic acid was the predominant unsaturated fatty acid (38.74±0.75 g\/100g). Linoleic acid (1.83±0,01 g\/100g) was also present. Methylxanthines were quantified: caffeine (0.118 ± 0.001) and theobromine (0.0125 ± 0.0002) g\/100g. The total polyphenols content was 0.717 ± 0.001 g\/100g. The results obtained in this work suggest the potential value of the fruits of Ilex paraguariensis for the development of novel products in the food and pharmaceutical industries. This paper aims to contribute to the scientific knowledge of a natural by-product from industry regarding the need of foods and medicines for the new millennium.","palabras_clave":"ilex paraguariensis|nutritional|phytochemical study"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1573,"idioma_id":4,"titulo":"Photothermal microscopy applied to the characterization of nuclear fuel pellets","resumen":"The photothermal photodeflection technique is shown to provide information on the homogeneity  of fuel pellets, pore distribution, clustering detection of pure urania and gadolinea and to provide a  two-dimensional mapping of the thermal diffusivity correlated to the composition of the interdiffused  Gadolinium and Uranium oxide. Histograms of the thermal diffusivity distribution become a reliable  quantitative way of quantifying the degree of homogeneity and the width of the histogram can be used  as a direct measure of the homogeneity. These quantitative measures of the homogeneity of the samples  at microscopic levels provides a protocol that can be used as a reliable specification and quality control  method for nuclear fuels, substituting with a single test a battery of expensive, time consuming and  operator dependent techniques.","palabras_clave":"Thermal diffusivity maps|Pore distribution and cluster detection|urania|gadolinea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1575,"idioma_id":4,"titulo":"Role of the parasympathetic nervous system in cardioprotection by remote hindlimb ischemic preconditioning","resumen":"              EL PRIMER Y SEGUNDO AUTOR CONTRIBUYERON POR IGUAL AL PRESENTE TRABAJO            This investigation was designed to determine the participation of the vagus nerve and muscarinic receptors in the remote ischemic preconditioning (rIPC) mechanism. New Zealand rabbits were anesthetized, and the femoral artery was dissected. After 30 min of monitoring, the hearts were isolated and subjected to 30 min of global no-flow ischemia and 180 min of reperfusion (non-rIPC group). The ventricular function was evaluated considering the left ventricular developed pressure and the left ventricular end diastolic pressure. In rIPC group, the rabbits were subjected to a three-cycle hind limb ischemia (5 min) and reperfusion (5 min) and the same protocol used in non-rIPC group was then repeated. In order to evaluate the afferent neural pathway during the rIPC protocol, we performed two different groups: one in which the femoral and sciatic nerves were sectioned and the other in which the spinal cord was sectioned (T9-T10 level). To study the efferent neural pathway during rIPC protocol, the vagus nerve was sectioned and, in a separated group, atropine was administered. The effect of vagal stimulation was also evaluated. An infarct size of 40.8±3.1% was obtained in non-rIPC group whereas in rIPC group, the infarct size decreased to 16.4±3.5% (p&lt;0.01). During preconditioning protocol, the vagus nerve section and the atropine administration each abolished the effect of rIPC on infarct size. Vagal stimulation mimicked the effect of rIPC, decreasing infarct size to 15.2±4.7% (p&lt;0.01). There was a trend for attenuation of the rIPC protection that was not statistically significant with the sections of femoral and sciatic nerves. However, we demonstrated the presence of a neural afferent pathway since the spinal cord section completely abolished the effect of rIPC on infarct size. In conclusion, rIPC activates a neural afferent pathway and the cardioprotective signal reaches the heart through the vagus nerve (efferent pathway) and acetylcholine activates the IPC phenomenon when acting on the muscarinic receptors.±3.1% was obtained in non-rIPC group whereas in rIPC group, the infarct size decreased to 16.4±3.5% (p&lt;0.01). During preconditioning protocol, the vagus nerve section and the atropine administration each abolished the effect of rIPC on infarct size. Vagal stimulation mimicked the effect of rIPC, decreasing infarct size to 15.2±4.7% (p&lt;0.01). There was a trend for attenuation of the rIPC protection that was not statistically significant with the sections of femoral and sciatic nerves. However, we demonstrated the presence of a neural afferent pathway since the spinal cord section completely abolished the effect of rIPC on infarct size. In conclusion, rIPC activates a neural afferent pathway and the cardioprotective signal reaches the heart through the vagus nerve (efferent pathway) and acetylcholine activates the IPC phenomenon when acting on the muscarinic receptors.","palabras_clave":"MYOCARDIAL INFARCTION|REMOTE ISCHEMIC PRECONDITIONING|VAGUS NERVE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1578,"idioma_id":4,"titulo":"Intrapatient emergence of OXA-247: a novel carbapenemase found in a patient previously infected with OXA-163-producing Klebsiella pneumoniae","resumen":"Two genetically related Klebsiella pneumoniae strains carrying  OXA-type carbapenemases were isolated from a single patient 1  month apart. Kpn163 harboured OXA-163 and Kpn247 a new  variant named OXA-247 that showed susceptibility to carbapenems  and expanded-spectrum cephalosporins similar to OXA-48.  Our epidemiological, biochemical and molecular results suggest  the intrapatient emergence of blaOXA-247 from blaOXA-163.","palabras_clave":"Carbapenemase|CHDL|Klebsiella pneumoniae|OXA-163"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1583,"idioma_id":4,"titulo":"Anti-tumor Effect of SLPI on Mammary But Not Colon Tumor Growth","resumen":"Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that was related to cancer development and metastasisdissemination on several types of tumors. However, it is not known the effect of SLPI on mammary and colon tumors. The aim of this studywas to examine the effect of SLPI on mammary and colon tumor growth. The effect of SLPI was tested on in vitro cell apoptosis and in vivotumor growth experiments. SLPI over-expressing human and murine mammary and colon tumor cells were generated by gene transfection. The administration of murine mammary tumor cells over-expressing high levels of SLPI did not develop tumors in mice. On the contrary, the administration of murine colon tumor cells over-expressing SLPI, developed faster tumors than control cells.Intratumoral, but not intraperitoneal administration of SLPI, delayed the growth of tumors and increased the survival of mammary but notcolon tumor bearing mice. In vitro culture of mammary tumor cell lines treated with SLPI, and SLPI producer clones were more prone toapoptosis than control cells, mainly under serum deprivation culture conditions. Herein we demonstrated that SLPI induces the apoptosisof mammary tumor cells in vitro and decreases the mammary but not colon tumor growth in vivo. Therefore, SLPI may be a new potentialtherapeutic tool for certain tumors, such as mammary tumors.","palabras_clave":"cancer|SLPI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1588,"idioma_id":4,"titulo":"Tandem repeats of the extracellular domain of Matrix 2 influenza  protein exposed in Brucella lumazine synthase decameric carrier molecule  induce protection in mice.","resumen":"The antigenic variation of influenza virus represents a major prevention problem. However, the ectodomain of the protein Matrix 2 (M2e) is nearly invariant in all human influenza A strains and has been considered as a promising candidate for a broadly protective vaccine because antibodies to M2e are protective in animal models. In this work we evaluated the possible use of Brucella abortus lumazine synthase protein (BLS), a highly immunogenic decameric protein, as a carrier of the M2e peptide. Chimeric proteins generated by the fusion of one or four in tandem copies of M2e to BLS were efficiently expressed in Escherichia coli and assembled in decameric subunits similarly to the wild type BLS enzyme, as demonstrated by the comparative circular dichroism spectra and size exclusion chromatography and static light scattering analysis. The M2e peptides were stably exposed at the ten N-terminal ends of each BLS molecule. Immunization of mice with purified chimeras carrying only one M2e (BLS-M2e) copy elicited a significant humoral immune response with the addition of different adjuvants. The fusion of four in tandem copies of the M2e peptide (BLS-4M2e) resulted in similar levels of humoral immune response but in the absence of adjuvant. Survival of mice challenged with live influenza virus was 100% after vaccination with BLS-4M2e adjuvanted with Iscomatrix(®) (P<0.001) and 80% when adjuvanted with alum (P<0.01), while the chimera alone protected 60% of the animals (P<0.05). The approach described in this study is intended as a contribution to the generation of universal influenza immunogens, through a simple production and purification process and using safe carriers that might eventually avoid the use of strong adjuvants.","palabras_clave":"INFLUENZA|VACCINE|MATRIX 2|BLS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1590,"idioma_id":4,"titulo":"A behavior-based approach for Educational Robotics activities","resumen":"Educational robotics proposes the use of robots as a teaching resource that enables inexperienced students to approach topics in fields unrelated to robotics. In recent years, these activities have grown substantially in elementary and secondary school classrooms and also in outreach experiences to interest students in STEM undergraduate programs. A key problem in educational robotics is providing a satisfactory, adequate easy-to-use interface between an inexpert public and the robots. This paper presents a behavior-based application for programming robots, and the design of robotic-centered courses and other outreach activities. Evaluation data show that over 90% of students find it easy to use. These activities are part of a comprehensive outreach program conducted by the Exact andNatural Science Faculty of University of Buenos Aires, Argentina (FCEN-UBA). Statistical data show that since 2009 over 35% of new students at the FCEN-UBA have participated in some outreach activity, suggesting their significant impact on student enrollment in STEM-related programs. ","palabras_clave":"Behavior-based robotics|educational robotics|mobile robots|robots programming interface|STEM outreach"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1593,"idioma_id":4,"titulo":"Behavioral responses to short- term transport in male and female Greater rheas (Rhea americana) reared in captivity","resumen":"ABSTRACT Animal transport is an indispensable  practice in species that need to be moved for management  or commercial purposes. However, transport may  have negative effects on individuals\u2019 welfare. The aims  of the present work were to determine if the behavioral  responses of adult Greater Rheas (Rhea americana)  bred in captivity are sensitive to short-term transport  and if males and females differ in their posttransport  behavioral activity and recovery. Eight males and 8 females  were placed in individual pens and allowed 6 d  to habituate (d 1 to 6) before transport procedure. On  the transport day (d 7), half of the birds (4 males and  4 females) were randomly assigned to a transport group  that was captured and handled to be placed into the  crates, exposed to a 30-min transport stressor, and immediately  returned to their pens. Four transports with  1 different male and female each time were performed.  The other half remained undisturbed and were used as  controls. Behavior of all individuals was video-recorded  during habituation days, after transport on d 7, and  on the 2 following days (d 8 and 9) to evaluate pre and  posttransport behavioral activity for 2 h per day.  No significant behavioral changes were observed during  the last 2 d of the habituation period (d 5 and 6), suggesting  that Greater Rheas were adapted to the housing  conditions before transport. After transportation,  several behaviors were affected: transported males and  females showed null resting, transported females also  showed reduced preening and increased vigilance (P  < 0.05), whereas transported males showed increased  drinking (P < 0.05) compared with their respective  control groups. The results suggest that behavioral  responses of captive-bred Greater Rheas are sensitive  to short-term transport (which includes handling) and  that males and females differ in their posttransport behavioral  activity, recovering their overall basal levels on  the third day posttransportation.","palabras_clave":"RATITE|STRESS RESPONSE|TRANSPORTATION|WELFARE|SEX DIFFERENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1595,"idioma_id":4,"titulo":"Efficacy of voriconazole in a murine model of acute Trypanosoma cruzi infection","resumen":"Objectives: Antifungal triazole derivatives have been studied as possible alternatives for the treatment of Chagas\u2019 disease. Voriconazole has demonstrated in vitro activity against Trypanosoma cruzi, but its efficacy in vivo has not yet been tested. We aimed to determine the effect of voriconazole in a murine model of acute T. cruzi infection.  Methods: Treatment efficacy was evaluated by comparing parasitaemia, mortality and organ involvement (by histological examination) of infected mice.  Results: Treatment with voriconazole significantly lowered parasitaemia and mortality compared with controls, reduced the percentage of mice with amastigote nests in heart and skeletal muscle and moderately decreased myocardial inflammation.  Conclusions: Our findings support the potential of voriconazole for the treatment of acute Chagas\u2019 disease and motivate future animal studies using varying doses and treatment schemes. Further evaluation of voriconazole for clinical use in human Chagas\u2019 patients is warranted.","palabras_clave":"Chagas disease|Benznidazole|Mouse model|Voriconazole"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1598,"idioma_id":4,"titulo":"Mathematical Model for Strategic Planning Optimization in the Pome Fruit Industry","resumen":"    production farm concerning varieties and planting densities. The model decides the optimal investment    policy for a given farm, maximizing the net present value of business while dynamically deciding its    planting structure along a given time horizon under different financing scenarios. The model constraints    impose restrictions on the activities to take into account risks and cultural practices. The mathematical    model corresponds to a mixed integer linear programming problem, where integer decisions are related    to the minimum reconversion land unit and funding requirements.    The model was applied to a realistic case study of a typical farm in the ??Alto Valle de Río Negro?? Argentine    region. The study was conducted over a 20-year time horizon considering four varieties of apples and    five of pears. The results showed the optimal investment policy for the replacement of varieties under    different scenarios, with and without external financing. A sensitivity analysis was also performed on    some of the most influential parameters. The model could be used either by governmental agencies to    advise private sectors and to develop strategic economic policies or by companies to optimize the business    profit.","palabras_clave":"STRATEGIC PLANNING|FRUIT INDUSTRY|OPTIMIZATION|RESTRUCTURING OF VARIETIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1601,"idioma_id":4,"titulo":"Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American Countries","resumen":"Spoligotyping is the most frequently used method for genotyping isolates of Mycobacterium bovis worldwide. In the current work, we compared spoligotypes from 1684 M. bovis isolates from Argentina (816), Brazil (412), Chile (66), Mexico (274) and Venezuela (116), obtained from cattle, humans, pigs, wild boars, farmed deer, goats, buffaloes, cats, and wild animals. A total of 269 different spoligotypes were found: 142 (8.4%) isolates presented orphan spoligotypes, whereas 1542 (91.6%) formed 113 different clusters. In cattle, SB0140 was the most representative spoligotype with 355 (24.6%) isolates, followed by SB0121 with 149 (10.3%) isolates. Clustering of spoligotypes ranged from 95.2% in Argentina to 85.3% in Mexico. Orphan spoligotypes were also variable, ranging from 23.7% in Mexico to 4.1% in Brazil. A large proportion of spoligotypes were common to the neighboring countries Argentina, Brazil and Chile. In conclusion, despite the diversity of spoligotypes found in the five countries studied, there are major patterns that predominate in these neighboring countries. These clusters may reflect a long-lasting active transmission of bovine tuberculosis or common historical origins of infection.","palabras_clave":"MYCOBACTERIUM BOVIS|SPOLIGOTYPES|LATIN AMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1606,"idioma_id":4,"titulo":"Evolution history of the crust underlying Cerro Pampa, Argentine Patagonia: Constraint from LA-ICPMS U-Pb ages for exotic zircons in the Mid-Miocene adakite","resumen":"This paper newly reports results of LA-ICPMS U?Pb dating for 282 zircon crystals separated from a Middle Miocene adakite in Cerro Pampa, southern Argentine Patagonia. With the exception of one spot age, 174 of the U?Pb concordia ages are markedly older (&gt;94 Ma) than the cooling ages of the adakite magma (ca. 12 Ma). The presence of numerous exotic zircon crystals indicates that the adakitic magma carries up information related to the crustal components during its ascent through the entire crust underneath Cerro Pampa. The obtained concordia ages of exotic zircons, 94?1335 Ma, are divisible into five groups having distinctive peaks on a population diagram. The first (94?125 Ma) and second age groups (125?145 Ma) correspond to the age of plutonic activities that formed the main body of the South Patagonian Batholith. The third to fifth groups respectively correspond to activities of the El Qumado-Ibañez volcanic complex (145?170 Ma), plutonic rocks scarcely exposed in Central Patagonia (170?200 Ma), and the Eastern Andean metamorphic complex of Late Paleozoic to Early Mesozoic ages (200?380 Ma). Our data suggest that the crust underneath Cerro Pampa was formed mostly after 380 Ma, the majority forming during the Early Cretaceous to Middle Jurassic. The processes of crustaldevelopment ceased for ca. 80 m.y. until the activity of the Cerro Pampa adakite in ca. 12 Ma. In contrast to the existence of numerous Archaean?Palaeoproterozoic exotic zircons in Mesozoic plutonic rocks distributed in Andean Cordillera at around 46°S, no evidence was found for Archaean?Paleoproterozoic crust on the Cerro Pampa region at 48°S. This evidence suggests that two crusts must have aggregated along a boundary between 46°S and 48°S with the continental margin of Gondwana during Late Paleozoic times, as part of the amalgamation of Pangea.","palabras_clave":"GEOCHRONOLOGICAL FINGERPRINT|ZIRCON|ADAKITE|ICPMS|CRUSTAL EVOLUTION|CERRO PAMPA|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1616,"idioma_id":4,"titulo":"Extended cold storage of cultured hepatocytes impairs endocytic uptake during normothermic rewarming","resumen":"During hypothermic preservation of cells (0-4°C), metab¬olism is diminished and energy-dependent transport processes are arrested. The effect of hypothermic preservation of hepatocytes in endocytic transport following rewarming has not been previously reported. We evaluated the uptake of EGF (Epidermal Growth Factor) ligand conjugated to fluorescent Quantum Dots (QDs) probes in rat hepatocytes after 24 and 72 h cold storage in University of Wisconsin (UW) solution at 4°C. QDs uptake was visualized during rewarming to 37°C under air or, in a second approach, at the end of rewarming under 5% CO2. After 24 h in UW solution, QDs were internalized under both rewarming conditions similar to non-preserved hepatocytes and cells maintained a normal cytoskeleton distribution. However, in hepatocytes preserved 72 h none of the cells internalized QDs, which remained bound to the membranes. After rewarming, this group showed diminished actin staining while viability was maintained at ~70%. Our results present evidence that, hypothermic preservation for 72 h in UW solution at 4°C does not prevent EGFR (Epidermal Growth Factor Receptor) activation but irreversibly impairs endocytic uptake upon EGF stimulation; presumably due to actin cytoskeleton disassembling. Our approach can be applied on other membrane receptor systems and with other hypothermic preservation solutions to understand the effect of cooling in endocytic transport and to determine the optimal cold storage period.","palabras_clave":"Endocytosis|Cold storage|Cultured hepatocytes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1617,"idioma_id":4,"titulo":"Hydration of alpha-pinene catalyzed by acid clays","resumen":"              The  hydration reaction of alpha-pinene in the presence of natural clays treated  with monochloroacetic acid as catalyst to obtain oxygenated compounds was  studied. Catalysts were characterized using X-ray diffraction, differential  thermal analysis, programmed thermal desorption of adsorbed pyridine, and  infrared spectroscopic analysis of adsorbed pyridine to determine Bronsted and  Lewis acid sites. Catalytic tests revealed that treatment of the natural clay  with the acid improved the catalytic activity and the selectivity toward  oxygenated products by increasing the acidity of the catalyst. The selectivity  toward oxygenated compounds increased with the augment of the alpha-pinene  conversion because of greater contact between water molecules with the  remaining alpha-pinene molecules. The natural clay without treatment produced  compounds resulting from alpha-pinene isomerization, whereas the treated clays  produced alcohols and other products in addition to isomerization compounds. After  a certain time, the alpha-terpineol was isomerized into cineols. Studies of the  reusability of the JAL catalyst were performed (clay treated with  monochloroacetic acid). As the number of reuses increased, the percent  conversion decreased; however, the selectivity toward oxygenated compounds increased      ","palabras_clave":"MONOCHLOROACETIC|HYDRATION|ALPHA PINENE|CINEOLS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1618,"idioma_id":4,"titulo":"Hierarchical linear mixed models in multi-stage sampling soil studies","resumen":"The issue of variances of different soil variables prevailing at different sampling scales is addressed. This topic is relevant for soil science, agronomy and landscape ecology. In multi-stage sampling there are randomness components in each stage of sampling which can be taken into account by introducing random effects in analysis through the use of hierarchical linear mixed models (HLMM). Due to the nested sampling scheme, there are several hierarchical sub-models. The selection of the best model can be carried out through likelihood ratio tests (LRTs) or Wald tests, which are asymptotically equivalent under standard conditions. However, when the comparison leads to a restricted hypothesis of variance components, standard conditions are not maintained, which leads to more elaborated versions of LRTs. These versions are not disseminated among environmental scientists. The present study shows the modeling of soil data from a sampling where sites, fields within sites, transects within fields, and sampling points within transects were selected in order to take samples from different vegetation types (open and shade). For soil data, several sub-models were compared using Wald tests, classic LRTs and adjusted LRTs where the distribution of the test statistic under the null hypothesis is the Chi-square mixture of Chi-square","palabras_clave":"Bulk density |Likelihood ratio test (LRT) |Texture|Total organic carbon"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1621,"idioma_id":4,"titulo":"Expression of DNA Methyl Transferase Genes in Four-Cell Bovine Embryos Cultured in the Presence of Oviducal Fluid","resumen":"The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell  stage). Real-time quantitative PCR was performed to determine the relative  expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas  DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression.","palabras_clave":"BOVINE EMBRYO|OVIDUCT|DNMT|DNA METHYLATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1631,"idioma_id":4,"titulo":"Understanding the inter-specific dynamics of two co-existing predators in the Tierra del Fuego Archipelago: the native southern river otter and the exotic American mink","resumen":"Knowledge about interactions between  endangered native southern river otters (Lontra provocax)  and introduced American mink (Neovison  vison) is essential for effective management of both  species. We evaluated competition for spatial and  trophic niches between otter and mink in overlapping  and non-overlapping areas, comparing distribution,  habitat preference, diet and mink marking behavior.  We surveyed otter and mink signs along 250 km of  Beagle Channel coastline. Habitat suitability models  were constructed based on species presence\/absence  and habitat characteristics, using generalized linear  models. Feces were collected for diet analyses. Otters  used forested coasts with 12?32 shoreline slope and  without human influence, and our evidence suggests  they were not affected by mink presence. Mink  preferred forested and shrubland coasts with 10?28  shoreline slope. Neither human influence nor otter  presence affected mink habitat occupation, but in the  presence of otters, mink left fewer signs. Otters  consumed more aquatic prey than mink, and mink  modified their diet in the presence of otters, consuming  more exotic small terrestrial mammals and less fish  as well as shifting to smaller and shallower fish species  that are less consumed by otters. Mink showed more  plastic, generalist behavior than otters, being more  tolerant of human presence, using more habitat types  and having greater diet breadth. At the same time,  otters apparently affect mink adversely and could help  limit their invasion in sympatric areas. Conservation  and recovery of otters, therefore, may produce a  secondary benefit of simultaneously reducing the  effect of mink, thereby providing an additional way  to control this exotic predator?s population.","palabras_clave":"Canal Beagle|Competencia|Especies invasoras|Lontra provocax|Neovison vison|Tierra del Fuego"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1636,"idioma_id":4,"titulo":"Emulsion Polymerization of Isoprene. Mathematical Model for Long-Chain Branching","resumen":"For a batch emulsion polymerization of isoprene at 10 °C in the presence of a chain transfer agent, a previous mathematical model [Minari et al., J. Appl. Polym. Sci. 2010, 116, 590] was extended for predicting the molecular weight distributions of all the generated long-branched molecular topologies; with each topology characterized by the number of tri- and tetra-functional branches per molecule. At the reaction end, and according to the new model predictions, the linear topology remains as the most abundant (with &#61566;50% of the total mass), followed by the single trifunctionally-branched topology (with &#61566;20% of the total mass). The model can be useful for developing strategies for controlling the distribution of branches\/molec., for estimating melt viscosities, etc.","palabras_clave":"polyisoprene|emulsion polymerization|molecular weight distribution|long chain branching"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1638,"idioma_id":4,"titulo":"Evaluation of the ELISA-F29 test as an early marker of therapeutic efficacy in adults with chronic Chagas disease","resumen":"This work compared the time at which negative seroconversion was detected by conventional serology (CS) and by the ELISA-F29 test on a cohort of chronic chagasic patients treated with nifurtimox or benznidazole. A retrospective study was performed using preserved serum from 66 asymptomatic chagasic adults under clinical supervision, and bi-annual serological examinations over a mean follow-up of 23 years. Twenty nine patients received trypanocide treatment and 37 remained untreated. The ELISA-F29 test used a recombinant antigen which was obtained by expressing the Trypanosoma cruzi flagellar calcium-binding protein gene in Escherichia coli. Among the untreated patients, 36 maintained CS titers. One patient showed a doubtful serology in some check-ups. ELISA-F29 showed constant reactivity in 35 out of 37 patients and was negative for the patient with fluctuating CS. The treated patients were divided into three groups, according to the CS titres: in 13 they became negative; in 12 they decreased and in four they remained unchanged. ELISA-F29 was negative for the first two groups. The time at which negativization was detected was significantly lower for the ELISA-F29 test than for CS, 14.5 ± 5.7 and 22 ± 4.9 years respectively. Negative seroconversion was observed in treated patients only. The results obtained confirm that the ELISA-F29 test is useful as an early indicator of negative seroconversion in treated chronic patients.","palabras_clave":"Chagas disease|Trypanosoma cruzi|Chemotherapy|Diagnosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1640,"idioma_id":4,"titulo":"Testosterone abrogates TLR4 activation in prostate smooth muscle cells contributing to the preservation of a differentiated phenotype","resumen":"Prostate smooth muscle cells (pSMCs) are capable of responding to inflammatory stimuli by secreting proinflammatory products, which causes pSMCs to undergo dedifferentiation. Although it has been proposed that androgens decrease proinflammatory molecules in many cells and under various conditions, the role of testosterone in the prostate inflammatory microenvironment is still unclear. Therefore, our aim was to evaluate if testosterone was able to modulate the pSMCs response to bacterial LPS by stimulating primary pSMC cultures, containing testosterone or vehicle, with LPS (1 or 10 µg\/ml) for 24-48h. The LPS challenge induced pSMCs dedifferentiation as evidenced by a decrease of calponin and alpha smooth muscle actin along with an increase of vimentin in a dose-dependent manner, whereas testosterone abrogated these alterations. Additionally, an ultrastructural analysis showed that pSMCs acquired a secretory profile after LPS and developed proteinopoietic organelles, while pSMCs preincubated with testosterone maintained a more differentiated phenotype. Testosterone downregulated the expression of surface TLR4 in control cells and inhibited any increase after LPS treatment. Moreover, testosterone prevented IêB-á degradation and the LPS-induced NF-kB nuclear translocation. Testosterone also decreased TNFa and IL6 production by pSMCs after LPS as quantified by ELISA. Finally, we observed that testosterone inhibited the induction of pSMCs proliferation incited by LPS. Taken together, these results indicate that testosterone reduced the proinflammatory pSMCs response to LPS, with these cells being less reactive in the presence of androgens. In this context, testosterone might have a homeostatic role by contributing to preserve a contractile phenotype on pSMCs under inflammatory conditions","palabras_clave":"PROSTATE SMOOTH MUSCLE CELLS|LPS|TLR4|TESTOSTERONE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1641,"idioma_id":4,"titulo":"Characterization of bovine MHC class II DRB3 diversity in Latin American Creole cattle breeds","resumen":"In cattle, bovine leukocyte antigens (BoLA) have been extensively used as markers for diseases and immunological traits. However, none of the highly adapted Latin American Creole breed have been characterized for  polymorphism by high resolution typing methods. In this work, we sequenced exon 2 of the BoLA class II DRB3 gene from 179 cattle (113 Bolivian Yacumeño cattle and 66 Colombian Hartón del Valle cattle breeds) using a polymerase chain reaction sequence based typing (PCR-) method. We identified 36 previously reported alleles and three novel alleles. Thirty-five (32 reported and three new) and 24 alleles (22 reported and two new) were detected in Yacumeño and Hartón del Valle breeds, respectively. Interestingly, Latin American Creole cattle showed a high degree of gene diversity despite their small population sizes, and 10  alleles including three new alleles were found only in these two Creole breeds. We next compared the degree of genetic variability at the population and sequence levels and the genetic distance in the two breeds  with those previously reported in five other breeds: Holstein, Japanese Shorthorn, Japanese Black,Jersey, and Hanwoo. Both Creole breeds presented gene diversity higher than 0.90, a nucleotide diversity higher than 0.07, and mean number of pairwise differences higher than 19, indicating that Creole cattle had similar genetic diversity at BoLA-DRB3 to the other breeds. A neutrality test showed that the high degree of genetic variability may be maintained by balancing selection. The FST index and the exact G test showed significant differences across all cattle populations (FST = 0.0478; p &lt; 0,001). Results from the principal components analysis and tree showed that Yacumeño and Hartón del Valle breeds were closely related each other. Collectively, our results suggest that the high level of genetic diversity could be explained by the multiple origins of the Creole germplasm (European, African and Indicus), and this diversity might be maintained by balancing selection.","palabras_clave":"BoLA-DRB3 polymorphism|major histocompatibility complex class II|Latin American Creole cattle|sequence-based typing|Yacumeño|Hartón del Valle"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1643,"idioma_id":4,"titulo":"Phenotypic variation of south-western Atlantic clam Mactra isabelleana (Bivalvia: Mactridae)","resumen":"The phenotypic shell shape variation of Mactra isabelleana was tested using the geometric morphometric method. Four  localities were sampled along the Rio de la Plata estuary and the coast of Buenos Aires province. Principal component analysis  and canonical variates analysis of the first principal components were performed to reveal the shell variation and differences  among localities, respectively. The specimens from different microhabitats mostly overlapped, although differences in shape  were observed in the development of the umbo, the enlargement of the dorsoventral axes and the elongation of the posterior  end. The ecological and physical parameters that could influence shell shape variation are discussed.","palabras_clave":"Taxonomia|Bivalvia|Mactridae|Morphology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1649,"idioma_id":4,"titulo":"Feeding impact of the planthopper Taosa longula (Hemiptera: Dictyopharidae) on water hyacinth, Eichhornia crassipes (Pontederiaceae)","resumen":"Taosa longula Remes Lenicov (Hemiptera: Dictyopharidae), a planthopper native to South America, is a candidate for the biological control of waterhyacinth, Eichhornia crassipes (Mart.) Solms- Laubach (Pontederiaceae), a serious weed worldwide. Biological control requires agents that are not only specific but also effective. Damage caused by sap-sucking insects is difficult to assess. In this work we designed an experimental and analytical procedure to evaluate the potential damage of T. longula on waterhyacinth. The damage that T. longula causes to the clonal reproduction, biomass production, and growth of waterhyacinth was studied through a paired greenhouse trial with floating cages. The performance of the plant, starting from two plants per treatment, was evaluated at different insect densities (5, 10, 15 and 20 nymphs per cage) until all the nymphs molted to adults. The tests showed that individual growth and biomass production of waterhyacinth was reduced due to the effect of the insect feeding above 5 nymphs per cage. The number of new plants produced by clonal reproduction was only significantly different above 15 nymphs per cage. These results suggest that this planthopper could be an effective agent for the biological control of waterhyacinth.","palabras_clave":"water hyacinth biocontrol|hemipteran feeding damage|paired feeding tests"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1663,"idioma_id":4,"titulo":"Effect of peanut skin extract on chemical stability and sensory properties of salami during storage","resumen":"BACKGROUND: Peanut skin extracts (PSEs) have proven antioxidant properties in different food products. The objective of thisstudy was to evaluate the effect of peanut skin extract as natural preserving compounds on chemical stability and sensoryproperties of salami during storage.RESULTS: PSEwas obtained with ethanol?water and added during the preparation of salami samples. Raw salami samples werecured and stored at 15 &#9702;C and 65% relative humidity. Moisture, peroxide value, conjugated dienes, free fatty acids and sensorydescriptive attributes were evaluated on the samples. Peroxide values increased during storage in all samples and were 82.9in control (salami without additives), 18.0 in salami with 0.2 g kg&#8722;1 PSE (E0.02), 13.0 in salami with 1.0 g kg&#8722;1 PSE (E0.1), and0.63 meqO2 kg&#8722;1 in salami with butylated hydroxytoluene (BHT) after 42 days of storage. BHT and E0.1 treatments resulted ina lower increase in the intensity of oxidized flavor and a lower decrease in the intensity of salami flavor.CONCLUSION: Chemical indicators and descriptive results indicated that PSE retards lipid oxidation and preserves sensoryproperties of salami, prolonging its shelf life.&#9702;C and 65% relative humidity. Moisture, peroxide value, conjugated dienes, free fatty acids and sensorydescriptive attributes were evaluated on the samples. Peroxide values increased during storage in all samples and were 82.9in control (salami without additives), 18.0 in salami with 0.2 g kg&#8722;1 PSE (E0.02), 13.0 in salami with 1.0 g kg&#8722;1 PSE (E0.1), and0.63 meqO2 kg&#8722;1 in salami with butylated hydroxytoluene (BHT) after 42 days of storage. BHT and E0.1 treatments resulted ina lower increase in the intensity of oxidized flavor and a lower decrease in the intensity of salami flavor.CONCLUSION: Chemical indicators and descriptive results indicated that PSE retards lipid oxidation and preserves sensoryproperties of salami, prolonging its shelf life.&#8722;1 PSE (E0.02), 13.0 in salami with 1.0 g kg&#8722;1 PSE (E0.1), and0.63 meqO2 kg&#8722;1 in salami with butylated hydroxytoluene (BHT) after 42 days of storage. BHT and E0.1 treatments resulted ina lower increase in the intensity of oxidized flavor and a lower decrease in the intensity of salami flavor.CONCLUSION: Chemical indicators and descriptive results indicated that PSE retards lipid oxidation and preserves sensoryproperties of salami, prolonging its shelf life.2 kg&#8722;1 in salami with butylated hydroxytoluene (BHT) after 42 days of storage. BHT and E0.1 treatments resulted ina lower increase in the intensity of oxidized flavor and a lower decrease in the intensity of salami flavor.CONCLUSION: Chemical indicators and descriptive results indicated that PSE retards lipid oxidation and preserves sensoryproperties of salami, prolonging its shelf life.","palabras_clave":"PEANUT SKIN|SALAMI|ANTIOXIDANT|SENSORI|STABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1666,"idioma_id":4,"titulo":"High lipid content of irradiated human melanoma cells does not affect cytokine-matured dendritic cell function","resumen":"Gamma irradiation is one of the methods used to sterilize melanoma cells prior to coculturing them with monocyte-derived immature dendritic cells in order to develop antitumor vaccines. However, the changes taking place in tumor cells after irradiation and their interaction with dendritic cells have been scarcely analyzed. We demonstrate here for the first time that after irradiation a fraction of tumor cells present large lipid bodies, which mainly contain triglycerides that are several-fold increased as compared to viable cells as determined by staining with Oil Red O and BODIPY 493\/503 and by biochemical analysis. Phosphatidyl-choline, phosphatidyl-ethanolamine and sphingomyelin are also increased in the lipid bodies of irradiated cells. Lipid bodies do not contain the melanoma-associated antigen MART-1. After coculturing immature dendritic cells with irradiated melanoma cells, tumor cells tend to form clumps to which dendritic cells adhere. Under such conditions, dendritic cells are unable to act as stimulating cells in a mixed leukocyte reaction. However, when a maturation cocktail composed of TNF-alpha, IL-6, IL-1beta and prostaglandin E2 is added to the coculture, the tumor cells clumps disaggregate, dendritic cells remain free in suspension and their ability to efficiently stimulate allogeneic lymphocytes is restored. These results help to understand the events following melanoma cell irradiation, shed light about interactions between irradiated cells and dendritic cells, and may help to develop optimized dendritic cell vaccines for cancer therapy.","palabras_clave":"MELANOMA|GAMMA IRRADIATION |DENDRITIC CELLS |LIPIDS|IMMUNOTHERAPY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1668,"idioma_id":4,"titulo":"Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration","resumen":"We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 105 colony-forming unit per millilitre (CFU\/mL)) when hydrocarbons are the attractants.","palabras_clave":"laser speckle|hydrocarbon biodegradation|chemotaxis|bacterial degradation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1672,"idioma_id":4,"titulo":"Antinociceptive activity of fruits extracts and 'arrope' of Geoffroea decorticans (chañar)","resumen":"Ethnopharmacological relevance: Geoffroea decorticans (chañar) fruits and their derivate product (arrope) have been traditionally used as food and a folk medicine for the treatment of a wide variety of diseases including bronchopulmonary disorders and to relieve dolorous process.         Aim of the study: In order to evaluate the pharmacology action of this plant, studies were performed of antinociceptive and antioxidant activities.         Materials and methods: The aqueous and ethanolic extracts and arrope of chañar were evaluated in various established pain models, including chemical nociception induced by subplantar formalin and intraperitoneal acetic acid and thermal nociception method, such as tail immersion test in rats. To examine the possible connection of the opioid receptor to the antinociceptive activity of extracts and arrope it was performed a combination test with naloxone, a nonselective opioid receptor antagonist.         Results: The aqueous extract and arrope (1000 mg\/kg) caused an inhibition of the pain in formalin test in the first phase, similar to morphine and decrease in the second phase. In a combination test using naloxone, diminished analgesic activity of aqueous extract and arrope were observed, indicating that antinociceptive activity is connected with the opioid receptor. The aqueous extract and arrope, caused an inhibition of the writhing response induced by acetic acid. Central involvement in analgesic profile was confirmed by the tail immersion test, in which the aqueous extract and arrope showed a significant analgesic activity by increasing latency time. The aqueous extract had more antioxidant activity than arrope, it may be due for the cooking process.         Conclusions: This study has shown that the aqueous extract and arrope of Geoffroea decorticans (chañar) fruits, does possess significant antinociceptive effects. It is further concluded that aqueous extract with maximum inhibition of free radical is the most potent extract amount tested extracts. At the oral doses tested the aqueous extract and arrope were non toxic. The present results justifying its popular use and constitutes the first validation study of the antinociceptive action.       ","palabras_clave":"GEOFFROEA DECORTICANS FRUITS AND ARROPE|ANTINOCICEPTIVE ACTIVITY|RATS|OPIOID SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1678,"idioma_id":5,"titulo":"Sitios de entierro de la localidad arqueológica Barrancas,  Maipú (Mendoza, Argentina)","resumen":"Desde mediados de 1940, el área de Barrancas, departamento de Maipú en la provincia de Mendoza, ha sido fuente de hallazgos de restos humanos. El presente trabajo tiene como objetivos realizar una actualización sintética de la información bioarqueológica de los sitios enterratorio recuperados previamente en el área y dar a conocer los hallazgos del sitio arqueológico B6, un enterratorio múltiple con características únicas en el área","palabras_clave":"Caracterización bioarqueológica|enterratorios|Mendoza"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1681,"idioma_id":4,"titulo":"Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients","resumen":"Despite Natural Killer (NK) cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltrating malignant tumors, especially in humans. NK cells infiltrating human colorectal cancers (CRC) were analyzed to identify their potential protective role in an antitumor immune response. Relevant molecule expression and functions were analyzed from different sources, comparing tumor associated NK cells (TANK) with autologous peripheral blood NK cells (PB-NK); and the latter, in comparison with PB-NK from normal donors. TANK displayed a profound alteration of their phenotype, with a drastic reduction of NK cell receptors expression. Co-culture experiments showed that CRC cells reduced activating NK cell receptors, and this resulted in poor IFN-ã production and degranulation. Also, PB-NK from CRC patients exhibited reduction in activating receptors expression and profound defects in the ability to activate degranulation and IFN-ã production. For the first time TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and exerting direct cancer cell killing. Taken together, our results suggest that the CRC patients had phenotypically and functionally altered NK cells, therefore supporting the progression of cancer.","palabras_clave":"PHENOTYPE|NATURAL KILLER CELLS|COLORECTAL|CANCER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1685,"idioma_id":4,"titulo":"Modelling potential impacts of Climate Change on the bioclimatic envelope and conservation of the Maned Wolf (Chrysocyon brachyurus)","resumen":"Forecasting the influence of climatic changes on the distribution of the Maned Wolf (Chrysocyon brachyurus) is important for the conservation of the species. We explored the environmental characteristics than best explain the current distribution of the species, modelled the past and present distribution, projected the niche model into the future, and identified suitable areas for conservation. Niche modelling was performed using Maxent and 21 environmental variables. For past conditions, we considered the Last Glacial Maximum (LGM) and the mid-Holocene (MH) climates. For future conditions, we used the A2a greenhouse gas emission scenario for 2050. Four General Circulation Models (FGOALS 1.0, HADCM3, IPSL-CM4 and MIROC 3.2) were used. The resulting niche model (AUC = 0.89±0.02) predicts maximum probability of presence at precipitation of 106 mm during the coldest quarter, of 396 mm during the warmest quarter, and in totally flat areas. The suitable area for the Maned Wolf currently covers 4,320,364 km2. For the LGM, there were inter-model differences in predicted areas (from 819,324 km2 to 6,395,886 km2) and in geographic location. The MH models showed drastic changes with respect to the present and considerable inter-model variation. Predictions for 2050 show significant (at least 33%) reductions in distribution. Only a minor fraction (39%) of the current distribution can be considered stable for the period LGM-2050. The FGOALS model was the best option for projecting species occurrence into the future because it included the three localities known for the Maned Wolf from the late Pleistocene and predicts stable areas that coincide with spatial patterns of genetic diversity. The FGOALS projection for 2050 predicts a 33% reduction in suitable habitats, indicating some stable areas (central South America) that will probably be key sites for the conservation of the species.","palabras_clave":"Canidae|Last Glacial Maximum|Maxent|Mid-Holocene|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1689,"idioma_id":5,"titulo":"Efecto  fitotóxico  de Baccharis  ulicina sobre la germinación y crecimiento inicial de Avena sativa, Lolium perenne y Raphanus sativus","resumen":"Baccharis ulicina es una maleza ampliamente distribuida en los pastizales de la zona semiárida argentina. A fin de evaluar sus posibles efectos alelopáticos, se utilizaron extractos acuosos de B. ulicina (hoja, tallo, raíz y planta entera) a dos concentraciones (50 y 150 g tejido\/L agua) sobre la germinación y crecimiento inicial de Avena sativa, Lolium perenne y Raphanus sativus. Para cada especie se regaron 100 semillas y se mantuvieron a 25°C y oscuridad. Se registraron la germinación de las semillas y la longitud de coleóptilo \/ hipocótile (C\/H) y radícula (R). Con los datos obtenidos se determinó: porcentaje de germinación, coeficiente de velocidad de germinación, vigor de la germinación, inhibición de la germinación, inhibición de la longitud de la radícula y del coleóptilo \/ hipocótile e índice de vigor de radícula y de coleóptilo \/ hipocótile.Los extractos con alta concentración redujeron la germinación en promedio 65% mientras que inhibieron la longitud en 58,8% (C\/H) y 93,3% (R) para A. sativa, 71,5% (C\/H) y 87,9% (R) para L. perenne, y 93,8% (C\/H) y 94,5% (R) para R. sativus. Se registró un evidente efecto fitotóxico de B. ulicina sobre las especies evaluadas, indicando que la alelopatía seria una estrategia ecológica de la especie.&nbsp;","palabras_clave":"Baccharis ulicina|alelopatia|germinacion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1696,"idioma_id":4,"titulo":"Perfringolysin O as a useful tool to study human sperm physiology","resumen":"Objective    To evaluate perfringolysin O, a cholesterol-dependent pore-forming cytolysin, as a tool to study several aspects of human sperm physiology.    Design    Prospective study.    Setting    Basic research laboratory.    Patient(s)    Human semen samples with normal parameters obtained from healthy donors.    Intervention(s)    Interaction of recombinant perfringolysin O with human spermatozoa.    Main Outcome Measure(s)    Assessment of perfringolysin O binding to spermatozoa, tests for acrosome and plasma membrane integrity, and acrosomal exocytosis assays.    Result(s)    Perfringolysin O associated with human spermatozoa at 4°C. The binding was sensitive to changes in cholesterol concentrations and distribution occurring in the plasma membrane of these cells during capacitation. When perfringolysin O\u2013treated sperm were incubated at 37°C, the plasma membrane became permeable, whereas the acrosome membrane remained intact. Permeabilized spermatozoa were able to respond to exocytic stimuli. The process was inhibited by proteins that interfere with membrane fusion, indicating that large molecules, including antibodies, were able to permeate into the spermatozoa.    Conclusion(s)    PFO is a useful probe to assess changes in the amount and distribution of the active sterol fraction present in the sperm plasma membrane. The toxin can be used for the efficient and selective permeabilization of this membrane, rendering a flexible experimental model suitable for studying molecular processes occurring in the sperm cytoplasm.","palabras_clave":"Perfringolysin O|Acrosome reaction|Human sperm"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1702,"idioma_id":5,"titulo":"Integración de Modelos de Planeamiento y Scheduling en Sistemas de Información Empresariales","resumen":"   La aplicación e implementación de modelos matemáticos tendientes a colaborar con la toma de decisiones no resulta un proceso sencillo y directo: existen diversos factores que dificultan la integración de los modelos matemáticos desarrollados con los sistemas de información empresariales. ERP 2 Optimizer Linkage (E2OL) es un sistema que acopla un modelo matemático al sistema empresarial o ERP utilizado, proveyendo las interfaces necesarias para la adecuada visualización y facilitando el modelado de problemas típicos presentes en la gestión de empresas de producción, al incrementar el empleo de los modelos de optimización de la planificación y scheduling en los procesos de manufactura de las empresas. ","palabras_clave":"Optimización|Planeamiento y Scheduling|Sistemas informáticos Empresariales|Integración"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1706,"idioma_id":4,"titulo":"Water surface temperature estimation from Landsat 7 ETM+ thermal infrared data using the generalized single-channel method:  Case study of Embalse del Río Tercero (Córdoba, Argentina)","resumen":"Monitoring of warm distribution in water is fundamental to understand the performance and functioning of reservoirs and lakes.  Surface water temperature is a key parameter in the physics of aquatic systems processes since it is closely related to the energy fluxes  through the water\u2013atmosphere interface. Remote sensing applied to water quality studies in inland waterbodies is a powerful tool that  can provide additional information difficult to achieve by other means. The combination of good real-time coverage, spatial resolution  and free availability of data makes Landsat system a proper alternative. Many papers have developed algorithms to retrieve surface temperature  (principally, land surface temperature) from at-sensor and surface emissivity data. The aim of this study is to apply the singlechannel  generalized method (SCGM) developed by Jime´nez-Mun˜oz and Sobrino (2003) for the estimation of water surface temperature  from Landsat 7 ETM+ thermal bands. We consider a constant water emissivity value (0.9885) and we compare the results with radiative  transfer classic method (RTM).  We choose Embalse del R&#305;´o Tercero (Co´rdoba, Argentina) as case study because it is a reservoir affected by the outlet of the cooling  system of a nuclear power plant, whose thermal plume could influence the biota\u2019s distribution and biodiversity. These characteristics and  the existence of long term studies make it an adequate place to test the methodology.  Values of estimated and observed water surface temperatures obtained by the two compared methods were correlated applying a simple  regression model. Correlation coefficients were significant (R2: 0.9498 for SCGM method and R2: 0.9584 for RTM method) while  their standard errors were acceptable in both cases (SCGM method: RMS = 1.2250 and RTM method: RMS = 1.0426). Nevertheless,  SCGM could estimate rather small differences in temperature between sites consistently with the results obtained in field measurements.  Besides, it has the advantage that it only uses values of atmospheric water vapor and it can be applied to different thermal sensors using  the same equation and coefficients.","palabras_clave":"Water surface temperature|Reservoir|Cooling system|Nuclear power plant|Single-channel generalized method|Atmospheric water vapor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1711,"idioma_id":5,"titulo":"Médano La Enriqueta: un lugar de entierro de cazadores tardíos sobre el río Colorado (Dpto. Caleu Caleu, provincia de La Pampa)","resumen":"EN ESTE TRABAJO SE PRESENTAN LOS PRIMEROS RESULTADOS OBTENIDOS SOBRE LAS EVIDENCIAS BIOARQUEOLÓGICAS Y CULTURALES RECUPERADAS EN EL SITIO MÉDANO LA ENRIQUETA I (MLE-I). ESTE SITIO CONSTITUYE UNA INTERESANTE MANIFESTACIÓN DE OCUPACIÓN PREHISPÁNICA DEL SECTOR SUR DEL DEPARTAMENTO CALEU-CALEU. LOS DATOS OBTENIDOS A PARTIR DE LA IMPLEMENTACIÓN DE DIFERENTES LÍNEAS DE ANÁLISIS SE DISCUTEN E INTEGRAN CON LOS ANTECEDENTES EXISTENTES PARA EL NORTE DE PATAGONIA Y SUR DE LA PROVINCIA DE LA PAMPA. MLE-I SE LOCALIZA SOBRE UN MÉDANO ACTIVO, EN EL CUAL SE IDENTIFICARON TRES HOYADAS QUE PARECERÍAN CORRESPONDERSE CON SECTORES DE USO DIFERENCIAL DEL ESPACIO POR PARTE DE LAS POBLACIONES PREHISPÁNICAS. EN SÓLO UNA DE ELLAS SE DETECTARON EVIDENCIAS DE LA INHUMACIÓN DE RESTOS HUMANOS, MIENTRAS QUE EN LAS RESTANTES DOS EL REGISTRO RECUPERADO ESTARÍA VINCULADO A OTRAS ACTIVIDADES. A PARTIR DEL ANÁLISIS DEL CONJUNTO ESQUELETAL, FUE POSIBLE IDENTIFICAR UN NMI DE 9. SE DESTACAN DOS CRÁNEOS CON DEFORMACIÓN INTENCIONAL DE TIPO TABULAR ERECTA, VARIANTE PLANOLÁMBDICA, CUYA CRONOLOGÍA RADIOCARBÓNICA, ASIGNADA AL HOLOCENO TARDÍO FINAL, CONCUERDA CON OTROS CASOS IDENTIFICADOS EN EL ÁREA. SE RECUPERÓ TAMBIÉN UNA DESTACADA VARIABILIDAD DE RESTOS CULTURALES PRINCIPALMENTE REPRESENTADOS POR ARTEFACTOS LÍTICOS, FRAGMENTOS CERÁMICOS Y RESTOS MALACOLÓGICOS. ","palabras_clave":"CAZADORES-RECOLECTORES|SE DE LA PAMPA|HOLOCENO TARDÍO|DISTRIBUCIÓN ESPACIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1739,"idioma_id":4,"titulo":"Effects of different anesthetics in the murine model of Equine herpesvirus 1 infection","resumen":"Mice are commonly used as an experimental model to investigate the Equid herpesvirus 1 (EHV-1) infection. This model easily reproduces the disease, and the clinical signs are more or less similar to those observed in the horse, the natural host. During natural infection, the acute course of respiratory infection is mandatory for the development of adaptive immune response. Since interactions between EHV-1 and anesthetics are possible, the study investigated whether the early events of murine pulmonary immune response could be affected by different anesthetics. Therefore, mice were experimentally infected with a unique EHV-1 strain under the effects of ether, ketamine\/xylazine, or isoflurane. Clinical signs and histopathological lesions in the lungs were described, and the cell death and proliferation rates of sham-inoculated or infected animals were quantified using immunohistochemistry. Clinical signs were more severe in animals anesthetized with ether. Qualitative differences in the recruited inflammatory cells were observed following application of anesthesia. The level of infection between the infected groups was not statistically significant. However, lungs from ketamine\/xylazine-anesthetized animals showed the highest cell death rates, whereas those from isoflurane-anesthetized animals showed the highest proliferation rates. It has been emphasized that anesthetics alone or their interactions with EHV-1 modify the response against the infection. An appropriate selection of the anesthetic during experimental studies is relevant to minimize wrong conclusions.","palabras_clave":"mice|anesthetics|Equine herpes virus 1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1746,"idioma_id":5,"titulo":"CARACTERIZACIÓN ELECTROQUÍMICA DE PROBETAS DE ALEACIONES Zn-Sn SOLIDIFICADAS DIRECCIONALMENTE EN FORMA HORIZONTAL","resumen":"En el presente trabajo se realizó el análisis de la resistencia a la corrosión de probetas de aleaciones Zn-1%Sn, Zn-2%Sn y Zn-3%Sn (porcentajes en peso) y de los metales puros Zn y Sn. Las mismas fueron obtenidas mediante el método de solidificación horizontal unidireccional, con extracción calórica en ambos sentidos. De cada una de las probetas se obtuvieron electrodos de trabajo de las zonas equiaxial, columnar y con transición columnar a equiaxial (TCE), correspondientes tanto a los cortes longitudinales  como transversales. Las probetas así obtenidas, fueron sometidas a desbaste mecánico hasta granulometría # 1200, con papeles de CSi, y luego se realizaron los ensayos de polarización en solución 0,5 M de ClNa, a temperatura ambiente. Para los ensayos se utilizó una celda convencional de tres electrodos, un electrodo de referencia de Calomel saturado, y como contraelectrodo se utilizó un electrodo de platino, en una celda electroquímica de vidrio Pyrex. Una vez finalizados los ensayos, se procedió al análisis superficial de las probetas. Se observó presencia de corrosión por picado, siendo más afectadas aquellas probetas que contienen un mayor porcentaje de Sn, en las cuales se presentaron pits de mayor tamaño y profundidad. Los bordes de las probetas fueron las regiones más afectadas a la corrosión por picado.","palabras_clave":"ZINC|ESTAÑO|CORROSIÓN|SOLIDIFICACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1747,"idioma_id":4,"titulo":"Modulation of glycolysis and the pentose phosphate pathway influences porcine oocyte in vitro maturation","resumen":"Glycolytic and pentose phosphate pathway (PPP) activities were modulated in porcine cumulus-oocyte complexes (COCs) during in vitro maturation (IVM) by the addition of inhibitors or stimulators of key enzymes of the pathways to elucidate their relative participation in oocyte maturation. The activities of glycolysis and PPP were evaluated by lactate production per COC and by the brilliant cresyl blue test, respectively. Glucose uptake per COC and the oocyte maturation rate were also evaluated. Lactate production, glucose uptake and the percentage of oocytes reaching metaphase II decreased in a dose-dependent manner in the presence of the pharmacological (NaF) or the physiological (ATP) inhibitors of glycolysis (p < 0.05). The addition of the physiological stimulator of glycolysis (AMP) caused no effect on lactate production, glucose uptake or the meiotic maturation rate. The pharmacological (6-AN) and the physiological (NADPH) inhibitors of PPP induced a dose-dependent decrease in the percentage of oocytes with high PPP activity and in the nuclear maturation rate (p < 0.05). The physiological stimulator of PPP (NADP) caused no effect on the percentage of oocytes with high PPP activity. The glycolytic and PPP activities of porcine COCs and maturational competence of oocytes seem to be closely related events. This study shows for the first time the regulatory effect of ATP and NADPH as physiological inhibitors of glycolysis and PPP in porcine COCs, respectively. Besides, these pathways seem to reach their maximum activities in porcine COCs during IVM because no further increases were achieved by the presence of AMP or NADP.","palabras_clave":"Glycolysis|Pentose phosphate pathway|In vitro maturation|Oocytes|Porcine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1751,"idioma_id":4,"titulo":"Environmental risk factors and perinatal outcomes in preterm newborns, according to family recurrence of prematurity","resumen":"OBJECTIVE: We analyzed the role of environmental risk factors, sociodemographic characteristics, clinical characteristics, and reproductive history in preterm births and their associated perinatal outcomes in families classified according to their histories of preterm recurrence among siblings.STUDY DESIGN: A retrospective study was conducted at Nuestra Señora de la Merced Maternity Hospital in the city of Tucumán, Argentina. A total of 348 preterm, non-malformed, singleton children born to multipara women were reviewed. The family history score described by Khoury was applied, and families were classified as having no, medium, or high genetic aggregation.RESULTS: Families with no familial aggregation showed a higher rate of short length of cohabitation, maternal urinary tract infections during the current pregnancy, and maternal history of miscarriage during the previous pregnancy. Families with a high level of aggregation had a significantly higher incidence of pregnancy complications, such as diabetes, hypertension, and immunologic disorders.CONCLUSION: Reproductive histories clearly differed between the groups, suggesting both a different response to environmental challenges based on genetic susceptibility and the activation of different pathophysiological pathways to determine the duration of pregnancy in each woman.Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.","palabras_clave":"preterm birth|familial aggregation|pregnancy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1764,"idioma_id":4,"titulo":"Conformational States of 2&#8242;&#8209;C&#8209;Methylpyrimidine Nucleosides in Single and Double Nucleic Acid Stranded Structures","resumen":"The hybridization performance of a set of 12-merRNA:RNA duplexes containing 2&#8242;-C-methyluridine, 5-bromo-2&#8242;-C-methyluridine, or (2&#8242;S)-2&#8242;-deoxy-2&#8242;-C-methyluridine was analyzed.Melting point temperatures of the modified duplexes showed an important &#916;Tm decrease (&#8722;8.9 to &#8722;12.5 °C), while circular dichroism experiments indicated that the helix was still A-type, suggesting a localized disturbance disorder. Molecular dynamics simulations using AMBER were carried out in order to gain structural knowledge about the effect of the 2&#8242;-C-methyl modification in double stranded environments. On the other hand, in an attempt to explain the behavior of the 2&#8242;-deoxy-2&#8242;-C-methyl nucleosides in single stranded environments, like the 10&#8722;23 DNAzyme core, molecular dynamic simulations were performed, incorporating the modified analogues into single stranded reported stem-loop structures, studding the sugar conformations along the MD trajectories. It was observed that, despite their preferential conformational states, the 2&#8242;-C-methyl analogues are flexible enough to adopt a different puckering in single stranded environments.","palabras_clave":"2'-deoxy-2'-C-methyluridine|2'-C-methyluridine|RNA duplexes|Molecular Dynamics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1766,"idioma_id":4,"titulo":"Searches for heavy long-lived sleptons and R-hadrons with the ATLAS detector in pp collisions at &#8730;s = 7 TeV","resumen":"A search for long-lived particles is performed using a data sample of   4.7 fb-1 from proton-proton collisions at a centre-of-mass energy   sqrt(s) = 7 TeV collected by the ATLAS detector at the LHC. No excess is   observed above the estimated background and lower limits, at 95%   confidence level, are set on the mass of the long-lived particles in   different scenarios, based on their possible interactions in the inner   detector, the calorimeters and the muon spectrometer. Long-lived staus   in gauge-mediated SUSY-breaking models are excluded up to a mass of 300   GeV for tan beta = 5-20. Directly produced long-lived sleptons are   excluded up to a mass of 278 GeV. R-hadrons, composites of gluino (stop,   sbottom) and light quarks, are excluded up to a mass of 985 GeV (683   GeV, 612 GeV) when using a generic interaction model. Additionally two   sets of limits on R-hadrons are obtained that are less sensitive to the   interaction model for R-hadrons. One set of limits is obtained using   only the inner detector and calorimeter observables, and a second set of   limits is obtained based on the inner detector alone.","palabras_clave":"ATLAS|high|energy|physics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1771,"idioma_id":5,"titulo":"Los Metatheria sudamericanos de comienzos del Neógeno (Mioceno Temprano, Edad-mamífero Colhuehuapense). Parte II: Microbiotheria y Polydolopimorphia.","resumen":"En este trabajo se encara la revisión sistemática de dos órdenes de Metatheria (Mammalia) sudamericanos de la Edad Colhuehuapense (Mioceno temprano): Microbiotheria y  Polydolopimorphia. En primer lugar se comentan y discuten las homologías de  las cúspides de los molares superiores e inferiores de los Argyrolagoidea  (Polydolopimorphia). En segundo lugar se ofrecen los resultados de un análisis  filogenético de los Microbiotheriidae (Microbiotheria). En tercer lugar, se  analizan los taxones colhuehuapenses de Microbiotheria y Polydolopimorphia, reconociéndose  dos especies nuevas: Orden Microbiotheria, Familia Microbiotheriidae: Pachybiotherium acclinum Ameghino, Pachybiotherium sp., Clenia minuscula Ameghino, Oligobiotherium divisum Ameghino, Eomicrobiotherium mykerum sp. nov., Microbiotherium sp. y Microbiotheriidae indet.; Orden Polydolopimorphia, Suborden Bonapartheriiformes, Superfamilia  Argyrolagoidea, Familia Argyrolagidae: Proargyrolagus  argentinus sp. nov. y Anargyrolagus  primus Carlini, Pascual y Goin; Familia Patagoniidae: Patagonia peregrina Pascual y Carlini; Argyrolagoidea indet. Finalmente,  se realiza una adición al conocimiento de los Didelphoidea colhuehuapenses,  describiendo una especie indeterminada de esta superfamilia, y un comentario  sobre la identidad de ?Microbiotherium?  gutierrezi Simpson, en el que se  reconoce la nueva combinación Coona gutierrezi.","palabras_clave":"Mioceno|Patagonia|Microbiotheria|Polydolopimorphia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1779,"idioma_id":4,"titulo":"Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor","resumen":"This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring.                     The biorecognition event aptamer-thrombin was detected from changes in the SPR angle produced as a consequence of the molecular interaction between the aptasensor and the target protein. The biosensing platform demonstrated to be highly selective for human thrombin even in the presence of large excess of bovine thrombin, bovine serum albumin, cytochrome C, lysozyme and myoglobin. The relationship between the changes in the SPR angle and thrombin concentration was linear up to 0.19 mmol.L&#8722;1 (R2= 0.992) while the detection limit was of 12.0 nmol.L&#8722;1 (240 fmol in the sample). This new sensing approach represents an interesting and promising alternative for the SPR-based quantification of thrombin.                   ","palabras_clave":"Thrombin binding aptamer|Aptasensor|Optical aptasensor|Thrombin biosensor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1784,"idioma_id":4,"titulo":"Spatial and temporal patterns in the feeding behavior of a fiddler crab","resumen":"Fiddler crabs consume the surficial microphytobenthos around their burrows during low tide. We studied the spatial and temporal feeding   patterns in the species Uca uruguayensis   by using sequences of digital pictures of feeding pellets accumulation.   Data from 61 crabs, feeding without the interference of neighbors, were   fitted to different models using Maximum Likelihood and Bayesian   approaches. Initial feeding location was independent from the emergence   location, and then, crabs continued holding a main feeding direction   (clockwise or counterclockwise), suggesting a systematic mechanism that   may avoid feeding over already processed sediment. Crabs used at least   half of their potential feeding area, but these areas were   heterogeneous. Both sexes developed similar feeding areas; however,   females were faster and needed less time to feed than males, suggesting   that males are time restricted. Our work also highlights the importance   of incorporating other underlying mechanisms of the behavior of species   into the study of feeding strategies.","palabras_clave":"FEEDING BEHAVIOUR|FEEDING RATE|FEEDING PATTERNS|FIDDLER CRABS|UCA URUGUAYENSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1791,"idioma_id":4,"titulo":"Areas of endemism in the southern central Andes","resumen":"This paper analyzes the distribution of vascular plants species endemic to the southern central Andes  (south-western Bolivia and north-western Argentina). All 540 species endemic to the study regions (approx.  720600 km2) have been included in the analysis. The main part of the endemic species is found in  semiarid habitats between 1500-3500 m asl pointing to the topographically complex plateau, slope, and  valley system of the southern central Andes as the main locations for its endemic flora. The distribution  of the endemic species within arid sites is in contrast with that of vascular plant diversity in general,  as the most diverse habitat of the region is the moist subtropical Tucumano-Bolivian Yungas forest of  the eastern Andes slope. A total of 17 well defined and partly overlapping distribution patterns were  indentified. The broadest distribution pattern defines a general area of endemism for the southern central  Andes. This area extends through nearly the entire region and is defined by species that are widespread  within the region in desert to sub-humid environments of the high Andes, slopes, or valleys. Nearly all  other areas of endemism are nested within this broad distribution pattern as successively north-south  overlapping areas along the slopes and valleys of the Andes and the Pampeanas Range. Despite the  distributional bias of endemism towards the arid sites almost half of the endemic species are restricted  to a few high endemic areas that lie in juxtaposition to the main rainfall zones. These areas contain the  widest habitat ranges in terms of altitude and rainfall within the region with the endemic species being  equally variable in altitude and moisture requirements. Previous defined phytogeographic units were not  recognized among the distribution patterns. However, the northern part of the Prepuna can be defined as  two partly overlapping distribution patterns.","palabras_clave":"AREAS OF ENDEMISM|ARGENTINA|BIOGEOGRAPHY|BOLIVIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1798,"idioma_id":4,"titulo":"Microbiological Contamination of Laboratory Mice and Rats Conventional Facilities in Argentina from 2001 to 2011","resumen":"Routine microbiological monitoring of rodent colonies in experimental animal facilities is essential to evaluate the health status of the animals used in investigations. Infections as a complicating factor in biomedical research are well-known. For many years, experiments failed because of the presence of diseases that killed animals during the assay or they produce interferences. In the present study, the microbiological contamination rates of mice and rats in conventional facilities in Argentina during 2001 to 2010 are reported. From 2001 to 2010, a total of 1755 mice and 939 rats from 122 conventional animal facilities were examined for the presence of selected microbial infections. The most frequent bacteria isolated in mice and rats were Pseudomonas aeruginosa and Proteus spp. The most important parasites identified were Syphacia spp. and Tritrichomonas spp. Serology assays demonstrated the highest prevalence for Mouse Hepatitis Virus (MHV) and Sendai virus in mice (HVJ), and Syalodacryoadenitis virus (SDA) and Mycoplasma pulmonis in rats. The results suggest that conventional facilities in Argentina should be renovated and monitored regularly to decrease microbiological contaminations.","palabras_clave":"CONTAMINATIONS|PATHOGENS|FACILITIES|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1850,"idioma_id":4,"titulo":"Tempol Blunts Afferent Arteriolar Remodeling In Chronic Nitric Oxide-Deficient Hypertension Without Normalizing Blood Pressure","resumen":"Renal preglomerular vessels play a central role in modulating renal function and injury, especially during conditions of renal hemodynamic stress such as hypertension. We evaluated whether improving the balance between nitric oxide (NO) and oxidative stress improves the morphological alterations of renal afferent arterioles that occur in NO deficiencyinduced hypertension.  We measured indices of NO and oxidative stress and evaluated renal morphology and afferent arteriolar remodeling in rats treated with vehicle, L-NAME or L-NAME plus tempol (a superoxide dismutase mimetic) for 6 weeks. L-NAME-treated rats had hypertension, lower urinary and renal NO indices, higher renal cortical levels of TBARS, GSSG and GSSG\/GSH. This was associated with significant eutrophic inward remodeling of the afferent arterioles; they had a marked decrease in arteriolar lumen area and a striking increase in arteriolar wall thickness and media to lumen ratio. Tempol did not significantly reduce blood pressure, but increased NO levels, decreased oxidative stress and partially blunted L-NAME-induced remodeling of afferent arterioles. L-NAME-induced remodeling of afferent arterioles is blunted by tempol. This beneficial effect on remodeling is associated with increases in NO indices, decreases in oxidative stress, without significant decreases in blood pressure. Thus, the balance between these components may contribute to the altered renal hemodynamics and function in this model.","palabras_clave":"NITRIC OXIDE|AFFERENT ARTERIOLE|OXIDATIVE STRESS|REMODELING, HYPERTENSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1888,"idioma_id":5,"titulo":". La arqueología del sector occidental de la región pampeana. Trayectoria y reposicionamiento respecto a la arqueología nacional.","resumen":"LA PROVINCIA DE LA PAMPA OCUPA UN LUGAR CENTRAL, DESDE LA GEOGRAFÍA, EN EL TERRITORIO NACIONAL. SIN EMBARGO EL CONOCIMIENTO SOBRE SU PASADO ARQUEOLÓGICO RECIÉN COMIENZA A DELINEARSE ALREDEDOR DE 1980. ACTUALMENTE EL CONOCIMIENTO ARQUEOLÓGICO DEL SECTOR HA ALCANZADO UN PROMISORIO DESARROLLO BAJO EL IMPULSO DE VARIOS GRUPOS DE TRABAJO Y EL APOYO DE INSTITUCIONES PROVINCIALES Y NACIONALES. A PARTIR DE ELLO, EL CONOCIMIENTO DEL PASADO PRE Y POSTHISPÁNICO, SE HA REPOSICIONADO RESPECTO AL INTERÉS QUE DESPIERTA PARA LOS PROPIOS INVESTIGADORES LOCALES Y REGIONALES, ASÍ COMO PARA LOS DE REGIONES COLINDANTES. A TAL PUNTO QUE MUCHOS DATOS Y RESULTADOS HAN LLEVADO A REPLANTEAR MACRO - REGIONALMENTE LOS SISTEMAS DE ORGANIZACIÓN SOCIAL, POLÍTICO, TECNOLÓGICO Y LA DEMOGRAFÍA DE LAS SOCIEDADES PREEXISTENTES. EL NODO DE CAMINOS INDÍGENAS, ASENTAMIENTOS, PARADEROS Y CEMENTERIOS DONDE DEJARON SUS SEÑALES MILENARIAS POBLACIONES EN DIFERENTES MOMENTOS, HA PROMOVIDO EL DESARROLLO DE UN IMPORTANTE CORPUS DE INFORMACIÓN DESDE DISTINTAS ESPECIALIDADES Y TEMÁTICAS TALES COMO LA DINÁMICA DE POBLACIONES, BIOARQUEOLOGÍA, ARQUEOLOGÍA DEL PAISAJE, ARQUEOLOGÍA HISTÓRICA, PROCEDENCIA DE RECURSOS Y MATERIALES CULTURALES, INTERACCIÓN SOCIAL, ARQUEOBOTÁNICA, BIOGEOGRAFÍA, ECOLOGÍA ISOTÓPICA, TAFONOMÍA, ENTRE OTROS MUCHOS ABORDAJES VENIDOS Y POR VENIR. ESTO HA INCENTIVADO TAMBIÉN LA RELACIÓN ENTRE LOS INVESTIGADORES Y LAS COMUNIDADES LOCALES, Y SU INTERÉS POR EL CONOCIMIENTO DEL PASADO, CONVIRTIÉNDOLOS EN GUARDIANES Y CUSTODIOS DE ESTE LEGADO.","palabras_clave":"CAZADORES- RECOLECTORES|TIPOS DE CONFLICTOS|PAMPA Y NORPATAGONIA|PROCESOS SOCIOHISTORICOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1920,"idioma_id":4,"titulo":"CHEMICAL CONSTITUENTS, ANTIINFLAMMATORY AND ANTIOXIDANT ACTIVITIES OF BARK EXTRACTS FROM PRUNUS TUCUMANENSIS LILLO","resumen":"The anti-inflammatory, antioxidant, antimicrobial and cytotoxic activities of the hexane (HE), chloroform (CE) and methanol (ME) extracts obtained from the bark of Prunus tucumanensis Lillo were investigated. Both ME and CE extracts displayed a significant in vitro anti-inflammatory activity similar to dexamethasone and to a commercial formulation (Pygeum) used for the treatment of benign prostatic hyperplasia (BPH). ME exhibited powerful antioxidant (67.6% relative to BHT) and free radical scavenging (RC50¼5 ppm) activities, antimicrobial activities against Staphylococcus aureus and Mycobacterium smegmatis and did not show any cytotoxic effect on human-derived macrophage cells. Chemical analyses showed that (2 R,3 R)-3,5,7,30,50-pentahydroxyflavan,  -sitosterol and  -sitosterol-3-O- -D-glucopyranoside (daucosterol) are relevant components of ME.","palabras_clave":"PRUNUS TUCUMANENSIS|ANTI-INFLAMMATORY ACTIVITY|ANTIOXIDANT ACTIVITY|CYTOTOXICITY|BENIGN PROSTATIC HYPERPLASIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1933,"idioma_id":5,"titulo":"Caracterización Microestructural De Materiales Compuestos Solidificados Direccionalmente","resumen":"Los compuestos de matriz metálica (MMCs) reforzados con partículas cerámicas ofrecen una alta resistencia y módulo de elasticidad, así como también buenas propiedades a alta temperatura comparadas con las de losmateriales convencionales. Las funciones que tiene un material de refuerzo en los MMCs son las siguientes: soportar las tensiones que se ejercen sobre el compuesto, aumentar las características mecánicas de la matriz, su dureza y resistencia al desgaste (sobre todo en el caso del refuerzo con partículas), mitigar los fallos de estas características con el aumento de la temperatura, frenar o detener la propagación de grietas a través del compuesto y el desarrollo de las fisuras. En el presente trabajo se analizan las fases presentes en los compuestos de matriz Zn-Al, reforzados con partículas de SiC y solidificados direccionalmente. Se observa la distribución del reforzante y la interacción de las partículas de SiC con la matriz de Zn-Al. La caracterización de la microestructura resultante se realiza mediante difracción de rayos X (DRX), microscopía óptica (MO) y microscopía electrónica de barrido (MEB).","palabras_clave":"materiales compuestos|aleaciones metálicas|solidificación direccional |microesstructuras"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1960,"idioma_id":5,"titulo":"Política ferroviaria, realidad económica y representaciones sociales: de las vísperas del peronismo a los años de Alfonsín. Una mirada en torno a 'Estación Perico' (Jujuy, Argentina)","resumen":"Los habitantes de la comunidad periqueña, como los de la provincia de Jujuy en general, fueron testigos de una realidad ferroviaria fruto de la conjunción de las políticas nacionales aplicadas al sector, la impronta de los procesos históricos en la región y las particularidades propias de estos espacios. Aunque para más de un contemporáneo el desenlace de principios de los noventa se presentó como una ?muerte anunciada?, no fueron pocos los que, a lo largo de los años, apostaron a un cambio y orientaron esfuerzos a identificar obstáculos y dimensionar el problema, buscando receptividad en la opinión pública y\/o en las esferas gubernamentales. A partir tanto de la identificación de voces de actores sociales individuales y colectivos plasmadas en fuentes escritas (informes y discursos oficiales, diarios y revistas, petitorios elevados a los poderes públicos desde la sociedad civil) como de la recuperación de fragmentos de ?memoria ferroviaria? que subsisten en algunos habitantes de la localidad jujeña de ?Ciudad Perico?, el presente artículo propone una aproximación a la incidencia de las políticas ferroviarias sobre realidades económico-sociales regionales singulares y a las representaciones tejidas en torno a la situación del ferrocarril en la provincia norteña, en un marco temporal que recorre desde los años previos a la irrupción del peronismo ?signados aún por las secuelas de la crisis económica mundial- hasta finales de la década de 1980, tiempo previo a la instalación sin reservas del neoliberalismo en el horizonte político y económico de gran parte de la dirigencia a lo largo del país, que puso punto final a cualquier expectativa de recuperación y apuntalamiento del transporte ferroviario.","palabras_clave":"ESTACIÓN PERICO|JUJUY|POLÍTICA FERROVIARIA|REPRESENTACIONES SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1963,"idioma_id":4,"titulo":"Heterochromatin type, amount and distribution in wild species of chili peppers (Capsicum, Solanaceae)","resumen":"Triple staining with the fluorochromes chromomycin A3, distamycin A and 4?-6-diamidino-2-phenylindole (CMA\/DA\/DAPI) was applied to somatic metaphases and interphase nuclei of 11 taxa of wild chili peppers (Capsicum), with 2n = 2x = 24 (C. annuum var. glabriusculum, C. cardenasii, C. chacoense, C. flexuosum, C. galapagoense, C. eximium, C. praetermissum and C. tovarii) and 2n = 2x = 26 (C. recurvatum, C. rhomboideum and C. villosum) to analyse heterochromatin type, amount and distribution in wild members of this genus. Heterochromatic banding patterns allowed the identification of all the taxa examined and contributed to their taxonomic grouping. GC-rich heterochromatin (CMA+\/DAPI-) was typical in all taxa; only C. praetermissum possessed also AT-rich (CMA-\/DAPI+) and mixed GC- and AT-rich (CMA+\/DAPI+) bands. Heterochromatin amount (expressed as % of karyotype length) ranged between 1.72 (C. chacoense) and 16.82 (C. flexuosum) and was positively correlated with karyotype length in most of the taxa examined. Heterochromatin located mainly at terminal position of chromosomes but intercalary position prevailed in C. flexuosum. Nucleolus organizer regions (NOR)-associated GC-rich heterochromatin was exclusively terminal and included the distal macrosatellite and a small portion on the corresponding arm. In all the taxa analysed, an equilocal heterochromatin distribution in non-homologous chromosomes of karyotype was observed, suggesting concerted evolution of heterochromatin dispersion in Capsicum.","palabras_clave":"Capsicum|Cytotaxonomy|Fluorescent chromosome banding|Karyotype evolution|Solanaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1969,"idioma_id":5,"titulo":"Reducción de dimensionalidad en la construcción de perfiles de gestión de la innovación","resumen":"El presente trabajo es una indagación de tipo exploratorio que pretende describir el perfil de cuatro sectores de empresas considerando los valores de la cultura, los rasgos de los líderes, la comunicación interna, en relación con la gestión de la innovación. Los sectores considerados son: Software y Electrónica, Producción Tradicional, Comercio y Turismo y los datos se obtuvieron de 45 empresas de la región de influencia de la UNICEN (Universidad Nacional del Centro de la Provincia de Buenos, Argentina). En la confección de los perfiles, se trabajó con el análisis de correspondencia múltiple y el biplot como métodos de reducción de dimensionalidad. Se pudieron identificar aspectos que caracterizan a los distintos sectores, surgiendo claras distinciones en los valores de la cultura y los rasgos deseables para los líderes. La forma habitual en que se transmite la información presenta la mayor variabilidad por sectores. Surge un ordenamiento de los diferentes sectores respecto a las actividades y los resultados de la innovación.","palabras_clave":"INNOVACIÓN|GESTIÓN|PERFILES|DIMENSIONALIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1972,"idioma_id":4,"titulo":"IMPACT OF CHANGING OXYGENATION POLICIES ON RETINOPATHY OF PREMATURITY IN A NEONATAL UNIT IN ARGENTINA","resumen":"Aims: To assess the impact of different oxygenation  policies on the rate and severity of retinopathy of  prematurity (ROP).  Methods: Between January 2003 and December 2006,  infants of 1500 g birthweight (BW) or less and\/or  32 weeks gestational age (GA) or less, and larger, more  mature infants with risk factors for ROP were examined  through three different time periods: period 1: high target  oxygen saturation levels (88?96%) and treatment at  threshold ROP; period 2: low target oxygen saturation  levels (83?93%) and treatment at threshold ROP;  period 3: low target oxygen saturation and treatment  at type 1 ROP.  Results: Type 1 ROP was detected more frequently in  babies of 32 weeks GA or less (50\/365, 13.7%) than in  more mature babies (15\/1167, 1.3%; p&lt;0.001). The rate  of type 1 ROP in period 1 was 6.9%; period 2, 3.6% and  period 3, 1.8%. Rates of stage 3 ROP declined over time  in both BW\/GA groups ( from 9.0% to 4.1% to 2.0%) as  did rates of plus disease ( from 7.5% to 3.6% to 1.8%).  Mean BW and GA declined from period 1 to period 3,  and death rates remained unchanged. 74.4% of babies  received all the examinations required; 48.1% of  treatments were undertaken after discharge from the  neonatal unit.  Conclusions: Lower target oxygen saturation was  associated with a lower rate of severe ROP without  increasing mortality, and changed the characteristics of  affected babies. Screening criteria need to remain wide  enough to identify all babies at risk of ROP needing  treatment.","palabras_clave":"RETINOPATHY|OXYGENATION|BLINDNESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1973,"idioma_id":4,"titulo":"Comparison of body size, relative growth and size at onset sexual maturity of Uca uruguayensis (Crustacea, Decapoda, Ocypodidae) from different latitudes in the south-western Atlantic","resumen":"Some crustaceans show variations of their reproductive biology within their geographical distribution, and knowledge about such variations is important for the comprehension of their reproductive adaptations. This study compared two populations of the fiddler crab Uca uruguayensis from two locations on the south-western Atlantic coast: Ubatuba Bay, São Paulo, Brazil and Samborombón Bay, Buenos Aires, Argentina. The population features analysed were the body size variation (carapace width = CW) and the size at the onset of sexual maturity (SOM) in order to test the hypothesis that the size at SOM, should be the same in relative terms (RSOM), independently of the latitudinal position. In the Brazilian population the CW ranged from 4.18 to 11.60 mm for males and 3.90 to 9.80 mm for females, and in the Argentinean population from 3.60 to 14.10 mm for males and 2.85 to 12.00 mm for females. In the Brazilian population the SOM was 7.1 (RSOM = 0.58) and 5.9 mm CW (RSOM = 0.57) for males and females, respectively, and in the Argentinean population it was 7.0 (RSOM = 0.42) and 6.75 mm CW (RSOM = 0.53) for males and females, respectively. This fact is probably related to a great plasticity in the life history features of Uca uruguayensis under different environmental conditions.","palabras_clave":"LATITUDINAL|VARIATION|FIDDLER CRAB|CRAB SIZE|ALLOMETRY|BRAZIL|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1984,"idioma_id":4,"titulo":"First nosocomial outbreak of VIM-16-producing Serratia marcescens in Argentina","resumen":"Seven metallo-&#946;-lactamase-positive isolates of Serratia marcescens   were recovered from three patients hospitalized in a neonatal ward in   an Argentinean hospital during the period July-September 2011. All the   isolates were multidrug-resistant, they belonged to a single clone, and   carried a blaVIM-16 -containing class I integron structure. This   represents the first nosocomial outbreak of metallo-&#946;-lactamase in Enterobacteriaceae in Argentina.","palabras_clave":"SERRATIA MARCESCENS|CABAPENEMASE|VIM|OUTBREAK|ENTEROBACTERIACEAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1985,"idioma_id":4,"titulo":"A method of screening for spike fertility in wheat","resumen":"Wheat grain yield is often associated with grain number\/m2. Spike fertility (SF), i.e. the quotient between grain  number and spike chaff dry weight, is a major component of grain number\/m2 determination. Several  methodologies have been proposed in the literature for field determination of SF, but they are tedious and  expensive. Also, no comparison between methodologies has been done. The feasibility of using wheat SF as  a selection criterion in a breeding programme or as a variable of interest in crop physiology studies depends  largely upon the availability of a simpler and faster method for collecting and processing samples. Thus, the  objective of the present study was to determine: (1) the association between SF calculated with the non-grain  spike dry weight at anthesis (reference method) or at crop maturity, (2) the association between SF evaluated  at the plot level (i.e. both non-grain spike dry weight and grain number determined as per area unit) and at the  individual spike level and (3) the minimum number of individual spikes that should be sampled for the  development of a screening method that can be applied in wheat breeding programmes or in crop physiology  studies. Associations between variables were determined by correlation analysis of treatment means, and by a  test of agreement for categorical rating (low, medium and high SF) between individual data of each variable.  Four experiments (BY95, BC96, BC97 and ML07) were performed with five, ten, eight and eight wheat  cultivars, respectively, under no environmental limitations, except for experiment ML07 which was not irrigated.  In the first three experiments, SF was determined both at the beginning of grain filling and at maturity, in plot-size  samples (0·8m2\/plot). In experiments BC96 and BC97, SF was determined both in plot-size samples and in  individual spikes (five spikes per plot), at the beginning of grain filling. In experiment ML07, increasing numbers of  individual spikes were sampled at maturity to assess SF. As a result: (1) a significant association (R2=0·78;  P0·05); (2) when comparing SF determined in large plot-size samples v. in  small samples of individual spikes, a good adjustment (R2=0·77; P0·05); and (3) increasing sample size from 5 to 40 spikes gradually decreased  the average relative standard error of the mean (from 0·034 to 0·012, respectively). In conclusion, wheat SF can  be determined in a fairly accurate way by sampling a small group of individual spikes at crop maturity, thereby  allowing the evaluation of a large number of treatments in a timely fashion and the screening of breeding material  from early generations.","palabras_clave":"WHEAT|SPIKE FERTILITY|SPIKE WEIGHT|SCREENING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":1988,"idioma_id":4,"titulo":"Behavioural responses of freshwater zooplankton vary according to the different alarm signals of their invertebrate predators","resumen":"Single behavioural interactions between two freshwater planktonic crustaceans and invertebrate predators from different zones of the commonenvironment were studied. The planktonic prey organisms were the cladoceran Ceriodaphnia dubia and the copepod Notodiaptomus conifer. The three invertebrate predators were the shrimp Macrobrachium borellii, the crab Trichodactylus borellianus and the larva of the dipteran midge Chaoborus. Feeding experiments were first performed to document the effects of exposure time and prey density on predation. All the selected predators fed on C. dubia and N. conifer, and predation rates were dependent on prey density and exposure time. The ability of microcrustaceans to modify their swimming and avoidance behaviour when faced with cues from each predator was then analysed. The cladoceran was more vulnerable to decapod predation, whereas the prey microcrustaceans were taken equally by the dipteran larvae. The analysis showed that themicrocrustaceans detected the presence of at least one invertebrate predator through the predator alarm signals. The cladoceran responded to chemical signals from the three predators but copepods modified their behaviour only in the presence of infochemicals of M. borellii. The different outcomes suggest that macrocrustaceans have different vulnerabilities when faced with the same predator and so have evolved specific and different strategies to reduce invertebrate predation.","palabras_clave":"PREDATOR-PREY INTERACTIONS|INFOCHEMICALS|BEHAVIOURAL EXPERIMENTS|ZOOPLANKTON|PREY|CERIODAPHNIA|NOTODIAPTOMUS|PREDATORS|CHAOBORUS|TRICHODACTYLUS|MACROBRACHIUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2262,"idioma_id":4,"titulo":"Larvicidal activity of crude extracts from Larrea cuneifolia (Zygophyllaceae) and its metabolite the nordihydroguaiaretic acid, against the vector Culex quinquefasciatus (Diptera: Culicidae).","resumen":"Introduction: The aim of the present study was to analyze the larvicidal activity of different crude extracts of Larrea cuneifoliaand its most abundant lignan, nordihydroguaiaretic acid (NDGA), against Culex quinquefasciatus. Methods: Chloroform,methanol, and aqueous extracts from L. cuneifolia and NDGA were tested against larvae of Cx. quinquefasciatus under laboratoryconditions. Results: The chloroform extract showed the highest larvicidal effect, with an estimated LC50 of 0.062 mg\/ml. NDGAalso demonstrated significant larvicidal activity with an estimated LC50 of 0.092 mg\/ml. Conclusions: These results indicate thatthe chloroform extract of L. cuneifolia and NDGA are promising insecticides of botanical origin that could be useful for controllingCx. quinquefasciatus.","palabras_clave":"LARREA CUNEIFOLIA|CULEX QUINQUEFASCIATUS|LARVICIDAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2445,"idioma_id":5,"titulo":"Análisis del registro arqueofaunístico de grupos cazadores recolectores del holoceno tardío de la llanura aluvial del Paraná medio (sitio Los Bananos, departamento Goya, Corrientes, Argentina)","resumen":"En este trabajo se presentan los resultados del estudio de los restos arqueofaunísticos procedentes del Sitio Los Bananos (Goya, Corrientes, Argentina). Los mismos evidencian la ocupación por parte de grupos cazadores-recolectores-pescadores en la llanura aluvial del Paraná medio durante el Holoceno tardío (1000 +20 años AP). Se realizó la estimación de la composición anatómica y taxonómica de los conjuntos. Se examinaron diferentes aspectos tafonómicos y se evaluaron las modificaciones antrópicas. Los resultados indican que los taxa que habrían sido más explotados por estos grupos están representados principalmente por aquellos característicos de los ambientes fluviales de la zona.","palabras_clave":"Sitio Los Bananos|Zooarqueología|Llanura aluvial del Paraná Medio|Holoceno tardío"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2483,"idioma_id":5,"titulo":"'Tomar la tierra, hacer ciudad. Estrategias de construcción de territorialidades sociales en tres casos de tomas de tierra en la ciudad de Córdoba, Argentina'","resumen":"En este artículo, se aborda un modo particular de ocupación del suelo yde creación de territorios populares: la ?toma de tierras?. Ésta, protagonizada por sectores que se mantienen en los márgenes de la normatividad urbana, inaugura un proceso de apropiación material y simbólica del espacio, configurando lo que denominamosterritorialidades sociales. Las mismas son entendidas como procesos eminentemente políticos que involucran dos aspectos centralese íntimamente relacionados: por un lado, la construcción de lascondiciones espaciales de habitabilidad (conflictos relacionados conel acceso al suelo, la producción de la vivienda y la obtención delos bienes colectivos asociados a la vida urbana); por otro, los modos de configuración de subjetividades que se activan a partir de lapregunta quiénes somos. Es a partir de ambos aspectos que nosacercamos a una interpretación del nosotros vinculado a la maneraen que los sujetos nominan, organizan y habitan el espacio barrial.Abordaremos este proceso a partir del análisis de tres tomas de tierra en la zona sur de la ciudad de Córdoba, Argentina: Toma Pueblos Unidos, Toma Comunidad Marta Juana González y Toma Obispo Angelelli; para ello, nos serviremos de la técnica de análisis decontenido sobre entrevistas realizadas a vecinos y referentes de organizaciones sociales que participan de las mismas.","palabras_clave":"Urbanización popular|Toma de Tierra|Territorialidades sociales|Subjetividad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2490,"idioma_id":4,"titulo":"Simple High-Performance Liquid Chromatography&#8722;Ultraviolet Method To Quantify the Molecular Size Distribution of Nonylphenol Ethoxylates","resumen":"Polyethoxylated nonylphenols (NPEOx) are widely used in various domestic and industrial applications. These commercial products are mixtures of oligomers that affect aquatic ecosystems, acting as endocrine disruptors. In the present work a simple high-performance liquid chromatography&nbsp; -ultraviolet method to quantify single oligomers of nonylphenol ethoxylates was developed. The following NPEOx commercial mixtures were analyzed: Igepal CO-520, Igepal CO-630, and Igepal CO-720.Obtained results demonstrate that the absorbance of each oligomer was not a function of the ethoxyl chain length. This result allowed the comparison between experimental molecular distribution functions of NPEOx mixtures with molecular distributions predicted by the Poisson equation. In all cases, good agreement was obtained. The method was validated by quantifying NPEOx samples with different molecular size distributions.","palabras_clave":"Nonylphenol Ethoxylates|Molecular Size Distribution| High-Performance Liquid Chromatography"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2493,"idioma_id":4,"titulo":"PGE2 Generation in Myocardium from Isolated Rat  Atrium under Hypoxia and Reoxygenation Conditions.  Effect of Anti-&#946;1 IgG from Patients with Chronic Severe  Periodontitis","resumen":"Background: Hypoxia is one of the most frequently encountered stresses in health and disease. Methods: We com-pared the effects of an anti-â1 periodontal IgG (pIgG) and an authentic â1 adrenergic agonist, xamoterol, on iso-lated myocardium from rat atria contractility. We used an ELISA assay to measure the generation of PGE2 in vitro after the addition of either the antibody or the adrenergic agonist. We analyzed the myocardium histopathological-ly in the presence of both the antibody and\/or the adrenergic agonist drug during normoxia, hypoxia and reperfu-sion conditions. Results: PGE2 generation increased during the hypoxia and was unchanged during reoxygenation period compared with the production of this prostanoid in atria during normoxia condition. A â1 specific adreno-ceptor antagonist atenolol and the â1 synthetic peptide abrogated the increment of the prostanoid in the presence of pIgG but only atenolol due to it in the presence of xamoterol. The increment of PGE2 was dependent on the activa-tion of cox-1 and cox-2 isoforms. Moreover, cox-2 was more active and produced more increments in the production of PGE2 in the presence of the pIgG than cox-1 activation. Histopathologically, studies of myocardium specimens during these different periods of the experimental protocol: basal (B), hypoxia (H) and reoxygenation (R), were also performed and showed tissue necrosis and edematization at the myocardium level. Conclusion: The phenomenon studied here supports the notion that PGE2 may be responsible for tissue edematization. PGE2 maybe acts as a beneficial modulator in the myocardium and prevents a major injury of it. The inflammation damage to the heart or- gan and cardiomyocytes caused by the actions of the antibodies in the course of heart lesions provoked by cardiovas- cular autoimmune disease, explains some of these results obtained in the present experiments. Further studies will be needed to establish the real role of PGE2 during hypoxia injury of the heart in the course of autoimmune diseases. ","palabras_clave":"MYOCARDIUM|PERIODONTAL|PROSTANOIDS|HYPOXIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2504,"idioma_id":4,"titulo":"'Determination of scandium in acid mine drainage by ICP-OES with flow injection on-line preconcentration using oxidized multiwalled carbon nanotubes'","resumen":"An on-line scandium preconcentration and determination system implemented with inductively coupled  plasma optical emission spectrometry associated with &#64258;ow injection was studied. Trace amounts of  scandium were preconcentrated by sorption on a minicolumn packed with oxidized multiwalled carbon  nanotubes, at pH 1.5. The retained analyte was removed from the minicolumn with 30% (v\/v) nitric acid.  A total enrichment factor of 225-fold was obtained within a preconcentration time of 300 s (for a 25 mL  sample volume). The overall time required for preconcentration and elution of 25 mL of sample was  about 6 min; the throughput was about 10 samples per hour. The value of the detection limit was  4 ng L1 and the precision for 10 replicate determinations at 100 ng L1 Sc level was 5% relative standard  deviation, calculated from the peak heights obtained. The calibration graph using the preconcentration  system was linear with a correlation coef&#64257;cient of 0.9996 at levels near the detection limits up to at least  10 mg L1  . After optimization, the method was successfully applied to the determination of Sc in an acid  drainage from an abandoned mine located in the province of San Luis, Argentina","palabras_clave":"SCANDIUM|ACID MINE DRAINAGE|OX-CNTs|ICP-OES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2505,"idioma_id":4,"titulo":"Chemoenzymatic synthesis of novel N-(2-hydroxyethyl)-&#946;-peptoid oligomer derivatives and application to porous polycaprolactone films","resumen":"Poly[N-(2-hydroxyethyl)-&#946;-propylamide] oligomer is synthesized using a simple enzymatic procedure involving Candida antarctica lipase B. This novel compound is obtained by a green and chemoselective method from economic reactants in good yield. The &#946;-peptoid oligomer is characterized by spectroscopic methods showing low molecular weight and low dispersity.  Two derivatives of the &#946;-peptoid oligomer are prepared by acetylation and by grafting polycaprolactone by ring opening polymerization from the pendant hydroxyl groups. These products are blendedwith polycaprolactone to make films by solvent casting. The inclusion of the acyl derivatives of the &#946;-peptoid to polycaprolactone affects the morphology of the film yielding  microstructured and nanostructured patterns.","palabras_clave":"lipase|&#946;-peptoid|polycaprolactone|films|surface properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2507,"idioma_id":5,"titulo":"Evaluación de cultivares de trigo 2013 y recomendaciones para la próxima campaña.","resumen":"Evaluación de variedades de trigo en cuatro fechas de siembra.","palabras_clave":"Trigo|variedades|siembra"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2510,"idioma_id":4,"titulo":"Granulomatosis with Polyangeitis. Rapidly progressive necrotizing glomerulonephritis in a pediatric patient","resumen":"VER PDF","palabras_clave":"Acute renal failure|Vasculitis|Crescentic pauci-immune glomerulonephritis-Type-C,|Antineutrophil cytoplasmic antibodies (c-ANCA)-macroscopic hematuria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2512,"idioma_id":4,"titulo":"Role of astrocytes in the immune-circadian signalling.","resumen":"n\/a","palabras_clave":"glia|neuroimmune|circadian"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2513,"idioma_id":4,"titulo":"Mycobacterium bovis &#916;mce2 double deletion mutant protects cattle against challenge with virulent M. bovis","resumen":"A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M.&nbsp;bovis Ämce2) was tested as an experimental vaccine in cattle challenged with a virulent M.&nbsp;bovis strain. Three-and-a-half-month old calves (n&nbsp;=&nbsp;5 to 6 per group) were vaccinated and challenged with a virulent strain of M.&nbsp;bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-ã by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-ã values similar to pre-vaccination except for the animals vaccinated with M.&nbsp;bovis Ämce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Ämce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M.&nbsp;bovis Ämce2 induced the highest (P&nbsp;&lt;&nbsp;0.05) expression of IFN-ã and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M.&nbsp;bovis Ämce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P&nbsp;&lt;&nbsp;0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M.&nbsp;bovis Ämce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M.&nbsp;bovis Ämce2 is a promising vaccine candidate to control M.&nbsp;bovis pathogenesis in cattle.","palabras_clave":"Bovine tuberculosis|Mycobacterium bovis|Live vaccine|mce"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2514,"idioma_id":4,"titulo":"Seismic characterization of the Quaternary sediments at Llancanello Lake Area","resumen":"A preliminary near-surface seismic survey was carried out at LlancaneloLake (Argentina) with the aim of mapping the quaternary layers, composed of sedi-mentary layers overlying basalt. A geological model has been obtained by interpretingthe events of a common-shot gather. Expressions of the traveltimes of the direct andrefracted waves versus o\u000Bset provide means to evaluate the velocities of the layers andthickness of the upper layer. Moreover, the standard processing sequence is appliedto the data to obtain a stacked section. This procedure reveals the location of deeperinterfaces, although it is highly a\u000Bected by the ltering of the direct and refractedevents, which have the same slope of the medium- and far off\u000Bset reflection signals.The fact that the direct arrival has not a zero intercept time indicates the pres-ence of a very thin layer at the surface (shallow salt deposits). Further evidence ofthe near-surface layer is that the refracted and reflection events are ringing and thereare strongly dispersive Rayleigh waves. An interface has been found approximately be-tween 54 and 57 m depth. The P- and S-wave velocities of the shallow layer are 2000and 550 m\/s, according to the slopes of the direct and Rayleigh waves, respectively,and rock-physics empirical relations, while the lower layer has a P-wave velocity of2800 m\/s, obtained from the refracted event. A tentative interpretation of the apexof a reflection event locates the top of the basalt layer at 190 m depth approximately.A viscoelastic model has been dened, with quality factors of typical volcaniclasticsediments. We then compute a synthetic common-shot gather and snapshots by meansof full-wave pseudospectral methods. The synthetic gather resembles the real one, pro-viding additional support to the interpretation.","palabras_clave":"Seismic processing |modeling |waves |interpretation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2515,"idioma_id":4,"titulo":"The Electrochemistry of Nanostructured Ni-W alloys","resumen":"This work reports on the features that Ni\u2013W nanostructuredalloys, electrodeposited on carbon steel by differentcurrent pulse programs, may present depending on their surfacemorphology and surface composition. The Ni\u2013W nanostructuredcoating, with a cauliflower structure, lack offragility, and high WO3\/W surface composition ratio, is astable electrode to catalyze hydrogen evolution reaction, exceedingbulk and electrodeposited Ni catalytic activity. Also,the nanostructured alloys must have a low WO3\/W surfacecomposition ratio for Ni and its oxides to provide protectionand improve corrosion resistance in sulfate media.","palabras_clave":"Pulse electrodeposition|Ni\u2013Walloys|Nanostructured materials|XPS|Corrosion resistance|Hydrogen evolution reaction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2516,"idioma_id":4,"titulo":"Antitumor properties of a vanadyl(IV) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: role of the oxidative stress and apoptosis","resumen":"Flavonoids, a polyphenolic compound family, and the vanadium compounds have interesting biological,pharmacological, and medicinal properties. We report herein the antitumor actions of the complex[VO(chrysin)2EtOH]2 (VOchrys) on the MG-63 human osteosarcoma cell line. Oxovanadium(IV), chrysinand VOchrys caused a concentration-dependent inhibition of cell viability. The complex was the strongestantiproliferative agent (p effect. Reactive oxygen species (ROS) and the alterations in the GSH\/GSSG ratio underlie the main mechanismsof action of VOchrys. Additions of ROS scavengers (vitamin C plus vitamin E) or GSH to the viabilityexperiments demonstrated beneficial effects (p of the mitochondria membrane potential (MMP), increased levels of caspase 3 and DNA fragmentationmeasured by the sub-G1 peak in cell cycle arrest experiments (p death modulator and a promissory complex to be used in cancer treatments.","palabras_clave":"flavonoid chrysin [VO(chrysin)2EtOH]2|osteosarcoma|apoptosis|oxidative stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2517,"idioma_id":4,"titulo":"High-precision U-Pb geochronology and a new chronostratigraphy for the Cañadón Asfalto Basin, Chubut, Central Patagonia: Implications for terrestrial faunal and floral evolution in the Jurassic","resumen":"The &#64258;uvial, lacustrine and tuffaceous sedimentary succession of the Cañadón Asfalto continental basin exposed in the Argentinean Chubut Province of central Patagonia preserves an extraordinary record of Jurassic fauna and &#64258;ora that marks key events in the evolution of Dinosauria, early mammals and major South American plant groups. However, basin-wide correlation of the fossiliferous units is complicated by fault displacements, unconformities and repetitive lithofacies. New U?Pb analyses of zircon (CA-TIMS method) from &#64257;ve primary tuff beds interstrati&#64257;ed with the lacustrine strata establish a new chronostratigraphic framework for the sedimentary andvolcanic units of Cañadón Asfalto Basin, constraining ca. 33 m.y. of depositional history and biotic evolution that spans nearly all three epochs of the Jurassic. Five major vertebrate- and plant-rich stratigraphic intervals have been identi&#64257;ed, and are being actively investigated, that range in age from Pliensbachian to Kimmeridgian (or younger). Our combined biostratigraphic and high-precision geochronologic results indicate that the major faunal turnover of the sauropodomorph dinosaurs which led to the rise of the eusauropods took place in theEarly Jurassic, earlier than previously recognized. Similarly, the &#64257;rst successful radiation of the ornithischian dinosaurs (heterodontosaurids), as well as the evolutionary diversi&#64257;cations of the araucarian conifers and the osmundaceous ferns, all occurred before the end of the Early Jurassic. The diverse palyno&#64258;oral assemblage of the Cañadón Calcáreo Formation that was once considered to be early Cretaceous in age, is now entirely constrained to the Late Jurassic.","palabras_clave":"Jurassic|U-Pb geochronolgy|Patagonia|Flora|Dinosaur|Stratigraphy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2518,"idioma_id":4,"titulo":"Conjugal transfer of a Sinorhizobium meliloti cryptic plasmid evaluated during a field release and in soil microcosms","resumen":"Horizontal gene transfer (HGT) is a central evolutionary mechanism that mediates the diversification and adaptation of bacteria in general and of rhizobia in particular. The few quantitative data on the conjugal transfer of rhizobial plasmids in soil correspond to the pSym (symbiotic genes-carrying replicons), with no information available regarding transfer frequencies in soil of other (namely accessory\/cryptic) plasmids that are present in several rhizobial species. Thus, we examined here the conjugal transfer in non-sterile soil of the model S. meliloti cryptic plasmid pSmeLPU88b. Under field conditions the proportion of nodules containing indigenous rhizobia that acquired the plasmid pSmeLPU88b and then nodulated the trapping plants could be estimated as < 0.1% (transconjugants\/nodule) over an 18-month sampling period that followed inoculation. The collected evidence showed that the release of rhizobia by means of standard seed-inoculation procedures did not result in a massive transfer of the introduced cryptic plasmid pSmeLPU88b to the indigenous bacteria that nodulate trapping alfalfa plants. Using a laboratory microcosm system performed with the same soil from the experimental field, we demonstrated that transconjugants were generated in the rhizosphere at a frequency of ca. 1.43 x 10-6 transconjugants\/recipient, a frequency from 102 to 103 16 times lower than that corresponding to the transfer of the same plasmid in rich-medium agar plates. The estimation of mobilization frequencies of rhizobial plasmids in soil is a necessary step towards the development of quantitative predictive models of gene-dispersal frequencies from inoculated strains to other rhizobia and soil bacteria.","palabras_clave":"field_release|cryptic_plasmid|rhizobia|legume"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2519,"idioma_id":4,"titulo":"On the validity of the general order kinetics for analizing phosphorescence curves","resumen":"The parameters characterizing trap and recombination  centers, such as activation energies, recombination and trap probabilities,  involved in thermoluminescent materials can be found by analyzing phosphorescence  curves with an adopted model consisting of trap and recombination centers, and assuming  the mechanisms involved in the carrier traffic (kinetics). Nowadays the most  employed kinetics is the General Order kinetics (GO). Since this model is  heuristic, this article reports how successful it is in providing reliable  values for the traps parameters. The equations describing the traffic of  carriers were used to generate simulated phosphorescence curves for specific  values of trap and recombination parameters. These simulated phosphorescence  curves were later analyzed with the GO kinetics, and the resulting parameters  compared to the correct ones in order to evaluate the validity of the model.","palabras_clave":"THERMOLUMINESCENCE|PHOSPHORESCENCE|DECONVOLUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2520,"idioma_id":5,"titulo":"Evaluación en laboratorio de un sistema sumergido de producción de endo-&#946;-1,4-glucanasa secretada por dos cepas de hongos de pudrición blanca","resumen":"La celulosa es el componente principal de la pared celular y es de gran importancia biotecnológica analizar nuevos microorganismos que produzcan celulasas. El objetivo de este trabajo fue diseñar y ensayar a nivel laboratorio un sistema de producción sumergido que utiliza chips de pino como sustrato para la producción de endo-1,4-&#946;-glucanasa secretada por dos hongos de pudrición blanca, Irpex sp. y Phlebia sp. y verificar el pH y T óptimos de acción de los extractos enzimáticos.  El sistema de producción propuesto en este trabajo mostró un comportamiento logarítmico respecto de la producción enzimática con un máximo de actividad de 1 U\/L.h para Irpex sp. y 1,37 U\/L.h para Phlebia sp. La actividad se mantuvo constante con el tiempo a través de lavados y agregado de medio fresco, simulando un sistema de producción fed-batch. Los extractos de ambas cepas mostraron una mejor performance a 50ºC, con una mayor actividad a pH 3 para los extractos de la cepa de Irpex sp. y a pH 4,5 para la cepa de Phlebia sp. Este sistema podría ser óptimo para iniciar los experimentos de escalado.","palabras_clave":"HONGOS DE PUDRICION BLANCA|SISTEMA DE SUSTRATO SUMERGIDO|ENDO-1,4 -GLUCANASA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2521,"idioma_id":5,"titulo":"DESARROLLO DE GALLETAS CON SUSTITUCIÓN PARCIAL DE HARINA DE TRIGO CON HARINA DE ALGARROBA (Prosopis alba) Y AVENA PARA PLANES SOCIALES","resumen":"La diversificación de alimentos es una estrategia para abordar problemas nutricionales. Producir  alimentos de consumo masivo incorporando harinas regionales sería una opción para obtener alimentos  de valor nutritivo optimizado. El objetivo de este trabajo fue desarrollar galletas de calidad nutricional  mejorada, para escolares, con mezclas de harinas de trigo, de algarroba y avena. Se determinó la  composición proximal y Ca, Fe, Mg, P, K y Zn en harina de algarroba con metodología AOAC y  disponibilidad potencial in vitro para Ca, Fe y Zn. Se evaluó la calidad proteica teórica de distintas  mezclas por el método del Puntaje Químico, previa corrección por digestibilidad, utilizando como  proteína de referencia los requerimientos del patrón FAO. Se diseñaron galletas con 3 mezclas  porcentuales: harina de trigo:harina de algarroba 70:30 y 80:20, harina de trigo:harina de algarroba:avena  RVCTA  Rev. Venez. Cienc. Tecnol. Aliment. 4(2):170-188. Macías, Sara et al. 171  80:10:10 y un testigo con 100 % harina de trigo. Se determinaron composición proximal, contenido y  disponibilidad potencial de Ca, Fe y Zn. Se midieron parámetros tecnológicos en masas y galletas  (color y factor de expansión). Las galletas se evaluaron sensorialmente con 35 consumidores, usando  escala hedónica de 9 puntos. El Puntaje Químico aumentó &#8776; el 25 % en la mezcla 70:30, 19 % en la  80:20 y 28 % para la 80:10:10 respecto del aminoácido lisina en harina de trigo. La corrección por  digestibilidad, posicionó con mejor calidad proteica a la mezcla 80:10:10. El diámetro de las galletas  aumentó con la disminución del espesor. El balance entre criterios nutricionales y tecnológicos  favoreció la elección de las galletas 80:20 y 80:10:10. Son fuente de fibra y minerales. En pruebas  sensoriales, las galletas obtuvieron puntaje superior a 6, siendo la más aceptada la 80:10:10. Es  tecnológicamente posible sustituir un 20 % de harina de trigo por los ingredientes propuestos  obteniéndose galletas nutricionalmente mejoradas y aceptables para los consumidores.","palabras_clave":"Dializabilidad |Galletas|Harina de algarroba|Minerales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2522,"idioma_id":4,"titulo":"Alveolar bone loss associated to periodontal disease in lead intoxicated rats under environmental hypoxia","resumen":"Previously reported studies from this laboratory revealed that rats chronically intoxicated with lead (Pb) under hypoxic conditions (HX) impaired growth parameters and induced damages on femoral and mandibular bones predisposing to fractures. We also described periodontal inflammatory processes under such experimental conditions. Periodontitis is characterised by inflammation of supporting tissues of the teeth that result in alveolar bone loss. The existence of populations living at high altitudes and exposed to lead contamination aimed us to establish the macroscopic, biochemical and histological parameters consistent with a periodontal disease in the same rat model with or without experimental periodontitis (EP). Sixty female rats were divided into: Control; Pb (1000ppm of lead acetate in drinking water); HX (506mbar) and PbHX (both treatments simultaneously). EP was induced by placing ligatures around the molars of half of the rats during the 14 days previous to the autopsy. Hemi-mandibles were extracted to evaluate bone loss by histomorphometrical techniques. TNF&#945; plasmatic concentration was greater (p<0.01) in Pb and HX animals. TBA-RS content was significantly higher in gums of rats with or without EP only by means of Pb. The SMG PGE2 content increased by Pb or HX was higher in PbHX rats (p<0.01). Pb and HX increased EP induced alveolar bone loss, while Pb showed spontaneous bone loss also. In conclusion, these results show that lead intoxication under hypoxic environment enhanced not only alveolar bone loss but also systemic and oral tissues inflammatory parameters, which could aggravate the physiopathological alterations produced by periodontal disease.","palabras_clave":"Bone loss|Environmental hypoxia|Periodontitis|Lead poisoning"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2523,"idioma_id":5,"titulo":"Edición del Volumen 1 N º 1. Nuestro Cuerpo en Nuestra Música.","resumen":"El XI Encuentro de Ciencias Cognitivas de la Música se propone: Contribuir a la difusión del conocimiento de las Ciencias Cognitivas de la Música particularmente en Iberoamérica.  (2) Estimular el desarrollo de la investigación en la región en esta área pujante de indagación científica, en tanto movimiento interdisciplinario que ha permitido la exploración sistemática de tópicos que tradicionalmente no habían sido considerados aptos para ser abordados científicamente.  (3) Ofrecer un espacio para la formación de estudiantes de grado y de posgrado de carreras afines al área (estudiantes de música, psicología, musicoterapia, filosofía, educación, biología, computación, etc.)  (4) Promover el encuentro fluido entre investigadores abocados al estudio de la música desde diversas disciplinas (psicología cognitiva, antropología cognitiva, educación, psicoterapia, biología evolucionista, filosofía, etc.), para el intercambio y la discusión de cuestiones teóricas y metodológicas.  (5) Favorecer el contacto directo de investigadores, docentes y alumnos iberoamericanos con referentes en el campo de las Ciencias Cognitivas de la Música regional e internacional.  (6) Ofrecer un espacio de difusión oral y escrita de las investigaciones llevadas adelante.","palabras_clave":"ECCOM|CIENCIAS COGNITIVAS|MÚSICA|EXPERIENCIA MUSICAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2524,"idioma_id":5,"titulo":"Sujeto y objeto en la investigación científica (parte I).","resumen":"Los autores desarrollan la relación sujeto-objeto en el acto de investigación","palabras_clave":"SUJETO DEL CONOCIMIENTO|OBJETO DEL CONOCIMIENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2525,"idioma_id":4,"titulo":"Islet neogenesis-associated protein (INGAP)-positive cell mass, &#946;-cell mass and insulin secretion: their relationship during the fetal and neonatal periods","resumen":"Objective: To study the chronological appearance of pancreatic Islet Neogenesis-Associated Protein (INGAP)-positive cells and its correlation with the increase in &#946;-cell mass and function in fetal and neonatal rats.   Methods: Normal Wistar rat embryos (E) at gestational days 15, 17 and 19 (E15, E17, E19) and 7-day-old postnatal rats (P7) were sacrificed to determine body and pancreas weight, blood glucose, glucose and arginine-induced insulin secretion in vitro, realtime PCR of Pdx1 and Ngn3, and quantitative immunomorphometric analysis of &#946;-cell replication and apoptosis rate, cytokeratin and INGAP cell mass, and Pdx-1- and Ngn-3-positive cells.   Results: Body and pancreas weight increased with age (P7>E19>E17>E15; P < 0.05). Neonates had higher blood glucose concentrations than embryos (P < 0.05). We recorded a simultaneous and significant age-dependent trend of increase in the number of &#946;- and Pdx-1-positive cells, &#946;- and cytokeratin-positive cell mass and &#946;-cell capacity to release insulin in response to glucose and arginine, and decreased &#946;-cell apoptotic rate. These changes closely paralleled the increase in INGAP-positive-cell mass. Conclusions: These findings suggest that INGAP exerts a positive modulatory effect upon &#946;-cell mass and its secretory function in fetal and neonatal rats, thus becoming a new component in the multifactorial regulation of such processes.","palabras_clave":"Islet Neogenesis-Associated Protein|ontogenesis|B cell function|B cell mass"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2527,"idioma_id":4,"titulo":"A chimeric cyclic interferon-alpha2b peptide induces apoptosis by sequential activation of phosphatidyl inositol 3-kinase, protein kinase Cdelta and p38 MAP kinase","resumen":"We have previously demonstrated that tyrosine phosphorylation of STAT1\/3 and p38 mitogen-activated protein kinase (p38 MAPK) activation are involved in the apoptotic response triggered by a chimeric cyclic peptide of the interferon-alpha2b (IFN-alpha2b) in WISH cells. Since the peptide also induced serine phosphorylation of STAT proteins, in the present study we examined the kinase involved in serine STAT1 phosphorylation and the signaling effectors acting upstream such activation. We first found that p38 MAPK is involved in serine STAT1 phosphorylation, since a reduction of phophoserine-STAT1 levels was evident after incubating WISH cells with cyclic peptide in the presence of a p38 pharmacological inhibitor or a dominant-negative p38 mutant. Next, we demonstrated that the peptide induced activation of protein kinase Cdelta (PKCdelta). Based on this finding, the role of this kinase was then evaluated. After incubating WISH cells with a PKCdelta inhibitor or after decreasing PKCdelta expression levels by RNA interference, both peptide-induced serine STAT1 and p38 phosphorylation levels were significantly decreased, indicating that PKCdelta functions as an upstream regulator of p38. We also showed that PKC&#61540; and p38 activation stimulated by the peptide was inhibited by a specific pharmacological inhibitor of phosphatidylinositol 3-kinase (PI3K) or by a dominant-negative p85 PI3K-regulatory subunit, suggesting that PI3K is upstream in the signaling cascade. In addition, the role of PI3K and PKCdelta in cyclic peptide-induced apoptosis was examined. Both signaling effectors were found to regulate the antiproliferative activity and the apoptotic response triggered by the cyclic peptide in WISH cells. In conclusion, we herein demonstrated that STAT1 serine phosphorylation is mediated by the sequential activation of PI3K, PKC&#61540; and p38 MAPK. This signaling cascade contributes to the antitumor effect induced by the chimeric IFN-alpha2b cyclic peptide in WISH cells.","palabras_clave":"CYCLIC PEPTIDE|MAPK|PI3K|INTERFERON ALPHA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2532,"idioma_id":4,"titulo":"Search for Displaced Muonic Lepton Jets from light Higgs boson decay in proton-proton collisions at &#8730;s = 7 TeV with the ATLAS detector","resumen":"A search is performed for collimated muon pairs displaced from the   primary vertex produced in the decay of long-lived neutral particles in   proton\u2013proton collisions at s=7 TeV centre-of-mass energy, with the ATLAS detector at the LHC. In a 1.9 fb&#8722;11.9 fb&#8722;1event   sample collected during 2011, the observed data are consistent with the   Standard Model background expectations. Limits on the product of the   production cross section and the branching ratio of a Higgs boson   decaying to hidden-sector neutral long-lived particles are derived as a   function of the particles&#700; mean lifetime.","palabras_clave":"ATLAS|High|Energy|Physics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2613,"idioma_id":4,"titulo":"Communities of anamorphic fungi on green leaves and leaf litter of native forests of Scutia buxifolia and Celtis tala: Composition, diversity, seasonality and substrate speci&#64257;city.","resumen":"Background: Xeric forests dominated by two tree species, Scutia buxifolia (Rhamnaceae) and Celtis tala (Ulmacea), are temperate, semi-deciduous wooded communities that represent the most abundant woodlands on the eastern plains of Buenos Aires Province, Argentina. The district of Magdalena has one of the most well-preserved native-forest areas, with an environmental heterogeneity that gives rise to the wide variability in the vegetation present.Aims: The aim of this study was to analyze the species composition, diversity, seasonal variations, and substrate speci&#64257;city of anamorphic fungi (Ascomycota) on the green leaves and in the leaf litter of native forests dominated by Scutia buxifolia and Celtis tala from Magdalena, Buenos Aires, Argentina. Methods: In order to obtain the mycobiota of decomposition, seasonal samples of green leaves and leaf litter from both types of trees were collected over a two-year period. In the laboratory, the leaves were placed in a moist chamber and incubated at room temperature. Results: A total of 100 species of anamorphic Ascomycota were identi&#64257;ed in both forests. No signi&#64257;cant variations were observed in the richness, diversity, or evenness of the fungal communities of the green leaves and leaf litter of both forests between seasons. Conclusions: The species that characterized the fungal communities in the leaves of each of the trees were found to be different. The type of substrate had a stronger in&#64258;uence in determining the composition of the fungal community in both types of forests.Keywords: Microfungi Xeric forest Leaf litter Decomposition","palabras_clave":"MICROFUNGI|XERIC FOREST|LEAF LITTER|DECOMPOSITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2620,"idioma_id":4,"titulo":"Water soluble nanocomposite films based on poly (vinyl alcohol) and chemically modified montmorillonites.","resumen":"Different montmorillonites were added to poly(vinyl alcohol) in order to improve their properties. The used montmorillonite?s were: Cloisite Naþ (Na), Nanofil (NF), and Cloisite 30B (30B). Poly(vinyl alcohol)  montmorillonite films,obtained by casting, were characterized by means of Fourier transform infrared spectroscopy, differential scanning  calorimetry, thermogravimetric analysis, X-ray diffraction pattern, transmission electron microscopy, water absorption,contact angle, and mechanical properties. Sodium and organically modified montmorillonites were used. The montmor-illonite basal peak shifted to a lower angle for composites, with sodium montmorillonites, and the films were exfoliated?  intercalated nanocomposites. Composites with organically modified montmorillonites presented a micro-morphology and the lowest water absorption. The best mechanical properties were obtained for the composite with sodium montmorillonites.","palabras_clave":"Poly(vinyl alcohol)|Montmorillonite|Organoclay|Characterization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2621,"idioma_id":4,"titulo":"Structure and development of the Andean system between 36° and 39°S","resumen":"tOne of the main morphological changes along the Southern Central Andes occurs from 36&#9702;to 39&#9702;S. Thenorthern portion is characterized by prominent basement structures and a thick-skinned orogenic frontwith relief of over 2000 m with a deep level of exhumation where more than 4 km of section has beeneroded. Contrastingly, the southern part is formed by mildly inverted basement structures restrictedmainly to the hinterland zone, which reaches only 1500?1700 m relief. We quantify the variable contrib-utions of two main contractional stages through the construction of three regionally balanced sectionsacross the Andes, constrained by field and geophysical data. Extensional re-activation described for thissegment in late Oligocene-early Miocene and Pliocene to Quaternary times, after the two main contrac-tional episodes, suggests only 3 km of stretching that represents 30?10% of the original longitude. We,therefore, conclude that while initial Late Cretaceous to Eocene compression was similar along strike(&#8764;10?7 km), it is the contrasting degrees of Neogene shortening (&#8764;16?6 km) that have played the largestrole in the along strike differences in structure and morphology along this portion of the southern Andes.Variable Neogene arc expansion could be responsible for the contrasting contractional deformation: Inthe north, late Miocene arc-related rocks cover most of the retroarc zone (&gt;200 km with respect to thelate Miocene arc front in the south), presumably driven by a shallow subduction episode in the area,whereas to the south they remain restricted to the continental drainage divide. Other factors involv-ing architecture of previous rift structures, are proposed as additional mechanisms that accommodatedvariable shortening magnitudes through inversion.","palabras_clave":"Southern Central Andes|Neogene contraction|Extensional reactivation|Fold and thrust belt"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2623,"idioma_id":5,"titulo":"Comportamiento de cultivares de sorgo granífero. Campaña 2012\/13.","resumen":"Evaluación de cultivares de sorgo sobre la base de los rendimientos de grano y el comportamiento agronómico.","palabras_clave":"Sorgo granífero|cultivares"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2624,"idioma_id":4,"titulo":"12.\tMicrowave assisted synthesis of ZnO tridimensional nanostructures","resumen":"The effect of different variables (reactive precursors, additives, stoichiometric ratio) on the final morphology of nano-ZnO  obtained by a microwave-assisted technique has been investigated. The characterization of the samples was carried out by  scanning electron microscopy in transmission mode (FE-SEM), infrared spectroscopy (FTIR) and x-ray diffraction (XRD). The  results showed that all the above-mentioned variables influenced to some extent, the shape and\/or size of the synthesized  nanoparticles. In particular, the addition of an anionic surfactant (sodium di-2-ethylhexyl sulfosuccinate, AOT) to the reaction  mixture allowed the synthesis of complex tridimensional particles with uniform size distributions and morphology.","palabras_clave":"NANOSTRUCTURES|MICROWAVE|ZNO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2625,"idioma_id":4,"titulo":"Drylands Development Model in Argentina?s Central West: the Case of Mendonza Province","resumen":"Argentina central west encompasses a vast dryland territory, organized on the basis of a great contradiction: the confrontation between oasis and rainfed area (desert lands with no irrigation). Within a territory under arid conditions with different desertification levels, Mendoza is a paradigmatic case. &#57417;e development model fostered at the end of the 19th century was based on the supremacy of trategic resources: water and soil. A mode of regional development reliant on irrigated lands was since then supported to consolidate the wine export model. Nowadays, Mendozas non-irrigated lands and their people are marginal, not merely by the effect of a restrictive environment but also by the combined action of a fragile environment and the weightier social, political and economic forces that have banished them to the systems margins. Analysis of the regions history informs that non-irrigated spaces provided both strategic natural resources for development of irrigated areas and labor for starting productive activities. Simultaneously, non-irrigated lands were curtailed in their right of access to strategic resources for their social reproduction: water and land.&#57417;is report delves further into the analysis of the dynamics of territory construction whereby some territories stand as central while others are relegated to marginal positions. &#57417;e analysis goes deeper into a case study with signs of a serious process of territory impoverishment, social exclusion and progress of desertification.","palabras_clave":"DRYLANDS|DESERTIFICATION|DEVELOPMENT MODEL|MENDOZA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2626,"idioma_id":4,"titulo":"A detection system with broad angular acceptance for particle identification and angular distribution measurements","resumen":"Nuclear Instruments and Methods A 726, 116 (2013)  \/\/\/  A new detection system for time-optimized heavy-ion angular distribution measurements has been designed and constructed. This device is composed by an ionization chamber with a segmented-grid anode and three position-sensitive silicon detectors. This particular arrangement allows identifying reaction products emitted within a 30° wide angular range with better than 1° angular resolution. As a demonstration of its capabilities, angular distributions of the elastic scattering cross-section and the production of alpha particles in the 7Li+27Al system, at an energy above the Coulomb barrier, are presented.","palabras_clave":"Ionization chamber|Position-sensitivedetector|Angular distribution|Particle identification|Straggling|7Li+27Al"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2628,"idioma_id":4,"titulo":"Service Selection Based on a Practical Interface Assessment Scheme (Indexed SCI, IF JCR2011: 1,615)","resumen":"Service-Oriented Computing promotes building applications by  consuming reusable services. However, facing the selection of adequate services for a specific application still is a major challenge. Even with a reduced set of candidate services, the effort on assessing candidates could be overwhelming. We have defined an approach to assist developers in the selection of services, which mainly comprises an assessment process for service Interface Compatibility. This assessment process is based on a comprehensive structural scheme for services´ interfaces matching. The scheme allows developers to gain knowledge on likely services´ interactions and their required adaptations to achieve a successful integration. We evaluated the performance of the Interface Compatibility analysis with a data-set of 453 services and two different service discovery registries. The experiments shown an improvement of up to 17% in precision and up to 8% in the DCG usefulness metric, with regard to the previous results obtained using only textual (syntactical) queries.","palabras_clave":"Service oriented Computing|Web Services|Interface Compatibility|Web Service Discovery|Web Service Selection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2630,"idioma_id":4,"titulo":"HYPOTHERMIC MACHINE PERFUSION VERSUS COLD STORAGE IN THE RESCUING OF LIVERS FROM NON-HEART-BEATING DONOR RATS","resumen":"            The aim of this work was to compare the preservation efficiency of  grafts excised from Non Heart Beating Donors that have suffered 45 min of warm  ischemia, employing Cold Storage (CS) and Hypothermic Machine Perfusion (HMP)  methods. We have developed a BES-Gluconate-Polyethylene Glycol based solution  (BGP-HMP), a new solution for HMP of livers for transplants. After 24 h of HMP  or CS, these livers were reperfused at 37°C with Krebs-Henseleit Dextran solution. In both  procedures, portal pressure and flow were measured and the intrahepatic  resistance (IR) was calculated. Perfusate pH oscillations and enzymes (LDH,  AST, and ALT) activities in perfusate were evaluated during normothermic  reperfusion. Also, the O2 consumption of the liver, glucose production  and the bile flow were measured during the normothermic reperfusion period.  Results: the portal flow (PF) and IR in both groups (n=5) showed statistical  differences (p&lt;0.05). HMP with BGP-HMP solution showed higher values of PF  and lesser IR than CS with HTK solution. Enzyme release after 90 min of  reperfusion didn´t show statistical differences between both groups. With  regard to bile flow and O2 consumption, preserved livers by both  process and solutions were able to produce bile, but the preserved livers with  HMP were able to uptake more O2 than livers preserved by CS.   ","palabras_clave":"HYPOTHERMIC PERFUSION|LIVER|COLD STORAGE|NHBD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2631,"idioma_id":4,"titulo":"Set membership estimation theory for coupled MIMO like Wiener models","resumen":"In this paper, an approach for identifying coupled multiple-input, multiple-output (MIMO) Wiener-like models is presented. Each multiple-input, single-output (MISO) model structure contained in the MIMO model is parameterized using finite sets of discrete Laguerre transfer functions followed by High Level Canonical Piecewise Linear (HLCPWL) that represents the static memoryless nonlinear block. For each MISO model, the parameters of the HLCPWL functions are found via Set-membership (SM) estimation theory, under mild error constraints. In this way, each MISO Wiener-like model is described as a set of parameters for the nonlinear static subsystem, whose values are ob-tained by solving a linear programming problem. The MIMO Wiener-like model structure is then rep-resented as a set of coupled input-output MISO models, converting the identification of a coupled MIMO system into the identification of MISO sys-tems. In order to validate the proposed identification algorithm, an illustrative example is provided.","palabras_clave":"WIENER LIKE-MODELS|NONLINEAR SYSTEMS IDENTIFICATION|HLCPWL FUNCTIONS|SET-MEMBERSHIPT ESTIMATION THEORY|UBB ERROR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2632,"idioma_id":4,"titulo":"Compositional dependence of the optical properties on amorphous Agx(Ge0.25Se0.75)100&#8722;x thin films","resumen":"The effect of silver content on the optical properties of films of Agx(Ge0.25Se0.75)100-x (with x &#8804; 25 at.%) chalcogenide glasses has been analysed. The films were prepared by pulsed laser deposition from bulk glasses target. Morphology, structure and optical properties of the films were characterised by profilometry, X-ray diffraction and UV-visible-NIR transmission spectroscopy. Refractive index, absorption coefficient, and the optical band gap energy were determined. It was found that the refractive index increases (for every wavelength) whereas the optical band gap energy decreases as the silver content increases.","palabras_clave":"Propiedades Opticas|Amorfos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2633,"idioma_id":4,"titulo":"THE EFFECTS OF PESTICIDES ON BACTERIAL NITROGEN FIXERS IN PEANUT GROWING AREA","resumen":"In the peanut production, the applications of herbicides and fungicides are a common practice. In this work, studies done under field conditions demonstrated that pesticides affected negatively the number and nitrogenase activity of diazotrophic populations of soil. Agrochemical effects were not transient, since these parameters were not recovered to pre-treatment levels even 1 year after pesticides application. Results obtained from greenhouse experiments revealed that the addition of herbicide or fungicides diminished the freeliving diazotrophs number reaching levels found in soil amended with the pesticides and that the number of symbiotic diazotrophs was not affected by the insecticide assayed. The soil nitrogenase activity was not affected by fungicides and glyphosate. The effect of pesticides on the nitrogen-fixing bacteria diversity was evaluated both in field and greenhouse experiments. Analysis of clone libraries generated from the amplification of soil nifH gene showed a diminution in the genetic diversity of this bacterial community.","palabras_clave":"Peanut |Pesticides |Soil diazotrophs|nifH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2634,"idioma_id":4,"titulo":"Macro and micro-environmental factors affecting tire-breeding flies (Insecta: Diptera) in urbanised areas","resumen":"    1. Urbanisation is often considered a main driver of changes in the    structure of insect communities. To assess macro- and microenvironmental factors    determining the occurrence and community structure of fly larvae in water-filled    artificial containers, a total of 13 848 individuals were collected among 1380 waterfilled    tyres inspected along an urbanisation gradient in temperate Argentina.    2. According to the best generalized linear mixed model obtained for fly occurrence,    the probability of finding an occupied tyre was greater in large tyres with high    water volume, containing organic matter and located in shaded conditions under high    vegetation cover in backyards. Regarding macroenvironmental factors, fly occurrence    decreased as the urbanisation level increased.    3. The anova revealed a negative effect of urbanisation on all variables used to    characterize the fly community structure. The number of occupied tyres, the relative    abundance of individuals, the species richness, and the Shannon diversity index    showed consistent patterns with significantly lower values at high urbanisation level.    4. Community composition also varied along the urbanisation gradient, revealing    three major groups of fly species: those found exclusively in highly urbanised areas,    those found in the less urbanised sites, and those collected across the entire gradient.  anova revealed a negative effect of urbanisation on all variables used to    characterize the fly community structure. The number of occupied tyres, the relative    abundance of individuals, the species richness, and the Shannon diversity index    showed consistent patterns with significantly lower values at high urbanisation level.    4. Community composition also varied along the urbanisation gradient, revealing    three major groups of fly species: those found exclusively in highly urbanised areas,    those found in the less urbanised sites, and those collected across the entire gradient.","palabras_clave":"urban ecology|richness|artificial containers| Culicidae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2635,"idioma_id":5,"titulo":"Demografía poblacional de Rhinella arenarum (Anura: Bufonidae) y Physalaemus biligonigerus (Anura: Leiuperidae) en agroecosistemas de la provincia de Córdoba, Argentina","resumen":"Abstract: Population demography in Rhinella arenarum (Anura: Bufonidae) and Physalaemus biligonigerus(Anura: Leiuperidae) in agroecosystems in the province of Córdoba, Argentina. The advancing agriculturalfrontier has led to an important loss of natural habitats, with significant consequences for biodiversity.The demography for two species of anurans, Physalaemus biligonigerus and Rhinella arenarum, both associatedwith agricultural systems in the central region of the Córdoba Province, were analyzed and compared in thisstudy. Four sites were sampled: three agroecosystems with different alteration degrees (C1, C2 and SM1) and afourth site not cultivated (SM2). The sampling was conducted during two reproductive periods, from September2008 to April 2009 and September 2009 to April 2010. Individuals were captured using live pitfall traps forthe metamorphic, juveniles and adults; and visual encounter survey, for the capture of eggs and larvae. Withthe abundance data, the survival for each age class was estimated using the KNM method (Kiritani NakasukiManly). With survival rates and fertility population, Leslie matrices were elaborated to obtain a quantitativeprojection of the population size. Altered environments showed lower eggs and larvae survival. Population projectionswere favorable in the site SM2 and were less favorable and a tendency to extinction, in sites dominatedby crops. This study showed that the agroecosystems of this region are possibly inhospitable environments forreproduction and survival of the species studied. The aquatic stages in the life cycle of both species would bethe more affected, since water bodies deterioration is present or may occur in those areas. We can recognizespecies-specific effects of agricultural ecosystems; P. biligonigerus was the most affected species, possiblybecause of their life histories and habitat requirements. We suggested that environmental degradation caused bythe cropland in the central region of Argentina would impact on the demographics of the anuran populations inthe area.","palabras_clave":"DEMOGRAFÍA POBLACIONAL|RHINELLA ARENARUM|PHYSALAEMUS BILIGONIGERUS|SUPERVIVENCIA|FERTILIDAD|AGROECOSISTEMAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2636,"idioma_id":5,"titulo":"Hidatidosis bovina:  Contrastación de los diagnósticos  macroscópico y microscópico","resumen":"El agente causal de la hidatidosis en nuestro país es el Echinococcus granulosus.  Este parásito toma como hospedadores definitivos a los cánidos y como intermediarios a los rumiantes, porcinos, especies selváticas y al hombre. En las plantas faenadoras durante la inspección de los órganos se decomisan  y eliminan todos aquellos con lesiones sospechosas de hidatidosis. Esto aporta importantes datos para conocer la enfermedad, su distribución, evaluar los planes de control y erradicación y valorar nuevas técnicas de diagnóstico y tratamiento Existen dos puntos críticos en los que el referido diagnóstico macroscópico casi invariablemente falla aquellos órganos en los cuales los metacestodes están presentes, pero no visibles macroscópicamente  y los que son decomisados por presentar lesiones similares a hidatidosis pero de etiología diferente. Este último aspecto es el que se estudió considerando como hipótesis la falta de relación entre los diagnósticos macroscópico  y microscópico. Los órganos con lesiones macroscópicas fueron remitidos al laboratorio, se seleccionaron las muestras, se caracterizaron  morfológicamente, topográficamente y se procesaron mediante las técnicas de rutina de inclusión en parafina y coloración con hematoxilina  y eosina. El diagnóstico histopatológico positivo se basó en la observación de la morfología de los quistes. Sobre un total de 105 muestras diagnosticadas como hidatidosis, 15 resultaron negativas al diagnóstico histopatológico.","palabras_clave":"HIDATIDOSIS BOVINA|DIAGNOSTICO MACROSCOPICO|DIAGNOSTICO MICROSCOPICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2638,"idioma_id":4,"titulo":"Fractal dimension as an index of left ventricular ischaemia: a pilot study","resumen":"Myocardial perfusion is performed by the left and the right coronary arteries, which deliver blood to the left and right ventricles, respectively. The impairment of arterial flow supply to the cardiac muscle by disease denotes a phenomenon known as ischaemia. Previous studies have demonstrated the ability of fractal dimension (FD) value of a physiological parameter in differentiating healthy\/pathological behaviours. The aim of this study consisted in quantifying the loss of ventricular thickness fractal complexity in order to determine if FD is an intrinsic marker of acute coronary ischaemia. Five mongrel dogs weighing 18.8-26.5 kg (24.4 ± 3.3, mean ± SD) were submitted to this studio. A left ventricular pressure transducer and a fluid-filled catheter for later calibration of the pressure transducer were introduced through a stab wound near the apex. Two pairs of ultrasonic microcrystals (5 MHz) for continuous wall thickness measurements were implanted at the anterior and posterior walls of the left ventricle following a previously described technique. During coronary occlusion, the ischemic wall started to thin at the very onset of relaxation (showing abnormal motility), while the normoperfused wall displayed postejective thickening. Concomitantly, posterior ventricular wall thickness and anterior wall ventricular thickness showed a significant decrease in its FD value (P &lt;0.05). In conclusion, loss of time series fractal complexity (waveform fine structure diminution or ´unwrinkling´) constitutes a marker of the presence of an ischemic process. As a result, a single scalar value is sufficient to characterize the entire behaviour of the time series. This value manifested a similar trend compared to the most well-known clinical indices of myocardial ischaemia.","palabras_clave":"CORONARY|FRACTAL|ISCHEMIA|INDEX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2686,"idioma_id":5,"titulo":"SANTA FE COMO VAMOS 2014","resumen":"Informe socioeconómico anual del convenio de Municipalidad de la ciudad de Santa Fe y la Bolsa de Comercio de Santa Fe.","palabras_clave":"SANTA |FE|COMO |VAMOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2709,"idioma_id":4,"titulo":"Arthropods on plants in a fragmented Neotropical dry forest: a functional analysis of habitat loss and edge effects","resumen":"Loss and fragmentation of natural ecosystems are widely recognized as the most important threats to biodiversity conservation, with Neotropical dry forests among the most endangered ecosystems. Area and edge effects are major factors in fragmented landscapes. Here, we examine area and edge effects and their interaction, on ensembles of insects associated to native vegetation in a fragmented Chaco Serrano forest. We analyzed family richness and community composition of herbivores, predators and parasitoids on three native plant species in 12 fragments of varying size and at edge\/interior positions. We also looked for indicator families by using Indicator Species Analysis. Loss of richness with the loss of forest area was observed for the three functional groups, with similar magnitude. Herbivores were richer at the edges without interaction between edge and area effects, whereas predators were not affected by edge\/interior position and parasitoid richness showed an interaction between area and position, with a steeper area slope at the edges. Composition of herbivore, predator and parasitoid assemblages was also affected by forest area and\/or edge\/interior situation. We found three indicator families for large remnants and five for edges. Our results support the key role of forest area for conservation of insect taxonomic and functional diversity in a highly threatened region, and emphasize the need to understand the interactions between area and edge effects on such diversity.","palabras_clave":"Area loss|Chaco Serrano| Edge effect|Functional groups"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2713,"idioma_id":4,"titulo":"Macrobenthos and multi-molecular markers as indicators of environmental contamination in a South American port (Mar del Plata, Southwest Atlantic)","resumen":"                                                            We evaluated benthic habitat quality along  a presumed contamination gradient in the Mar del Plata port (Southwestern Atlantic) by coupling biological and chemical proxies in  a multidisciplinary approach. Organic  matter and photosynthetic pigment contents were higher in silty-clay bottoms of  the inner port sites. Levels of all fecal  steroids decreased from the inner sites to the port inlet. High concentrations of coprostanol in the inner sites seemed to derive from a permanent  population of sea lions rather than from sewage outfalls due to  coprostanol\/epicoprostanol ratio (IV) values  High disturbance and low ecological status were reflected in low  benthic diversity and high AMBI values in the inner sites.                    ","palabras_clave":"SOFT-BOTTOM COMMUNITY|POLLUTION INDICATOR|MULTI-MOLECULAR MARKERS|ENVIRONMENTAL HEALTH|MAR DEL PLATA PORT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2714,"idioma_id":4,"titulo":"Determinants of immunological and virological responses to antiretroviral therapy amongst HIV-infected adults in central Argentina: negative influence of hepatitis C infection","resumen":"Background: The purpose of this study was to update the epidemiological data on the prevalence of coinfectionwith hepatitis Cvirus (HCV) and HIV, and to identify whether specific clinical and epidemiological factors influencedthe response of HIV-positive adults to highly active antiretroviral therapy (HAART).Methods: This retrospective observational cohort study of 238 HIV-infected patients evaluated the effect of differentepidemiological and clinical parameters (including HCVcoinfection) on therapy response among HIV-infectedadults initiating HAART. Multiple logistic regression models were used to identify factors associated with therapyresponse and estimated risk coefficients.Results: Seroprevalence of HCV infection in this population was 26% (62\/238).We did not observe a significant associationbetween immunological or virological response relating to patient gender or HAART regimen. However,this analysis showed that HCV serological status, age at HIV diagnosis, duration of treatment and WHO clinicalstage of AIDS (,200CD4cells\/ml independentlyof viral load either,or.to100000copies\/ml),were significantlyassociated with immunological and virological responses to HAART.Conclusions: These results showfurther evidence that hepatitis C serostatus is associated with a reduced responseto HAART.","palabras_clave":"CD4 cells counts|HAART|HIV\/HCV coinfection|response to therapy|viral load"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2715,"idioma_id":4,"titulo":"\u2022\tPGD in Carriers of reciprocal translocations by aCGH in trophectoderm biopsy and defferred cycle transfer","resumen":"Objective:It is well known that reciprocal and Robertsonian translocations carriers have increased risks of producing aneuploidies gametes associated with the meiotic multivalent, which ones abort spontaneously or end with the birth of an abnormal child. Method: We present our preliminary experience with 8 (eight) cases of PGD of balanced translocations with trophectoderm biopsy , genetic study by aCGH and deffered transfer. Seven cases were reciprocal translocations and only one was a Robertsonian translocation. From reciprocal translocations twenty two blastocysts were biopsied and ten from Robertsonian translocations.Results: Amongst the first group, a 41% resulted in a normal aCGH, a 46% abnormal due to malsegregation of the multivalent, and a 13% aneuploid not related to the meiotic quadrivalent . In the Robertsonian translocation a 60% was normal, a 10% unbalanced by malsegregation of the meiotic trivalent and a 30 % aneuploid not related to translocation. Surprisingly most of the abnormalities (90%) were due to adjacent type 2 segregation and malsegregation 3:1, which imply important chromosomal unbalance. Out of the 8 (eight) performed cycles, only 4 were transferred with a normal aCGH blastocyst. Three of them achieved the pregnancy. Conclusion: Taking into account the results achieved with biopsy in day 3 and fresh transfer, it seems that the results in day 5 and deferred transfer are much better. Probably the biopsy performed in an embryo that reach the maximum stage of development in vitro and the transfer in a cycle with an endometrium more physiologically prepared are the main reasons for the best results.","palabras_clave":"PGD|CARRIERS|TRANSLOCATIONS|ACGH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2716,"idioma_id":4,"titulo":"Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1","resumen":"se valuó la habilidad de una cepa de S.cerevisiae probiotica la capacidad de ligar in vitro fumonisina B1 . Se optimizó estadisticamente las condicones de óptimo crecimiento de la misma.","palabras_clave":"Ocratoxina|S.cerevisiae|DOE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2717,"idioma_id":4,"titulo":"Unexplained recurring high mortality of southern right whale calves (Eubalaena australis) at Península Valdés, Argentina","resumen":"Hundreds of southern right whale calves Eubalaena australis died on their calving ground at Península Valdés, Argentina from 2003 through 2011. During this period, the number of dead calves increased at a much greater rate than that of living calves over the preceding 32 yr, and with greater inter-annual variation. High mortality events occurred late in the calving seasons of 2005 and 2007, early in the seasons of 2008 and 2009, and were equally divided between early and late in 2010 and 2011. Calves that died late in the seasons of 2005 and 2007 were at least  a meter longer (mean 7.3 m) than newborns, indicating that they had grown and presumably were healthy before dying. An unusual number of large calves (>6 m) died early in the seasons of 2008 and 2009, suggesting that a population-wide process (e.g. nutritional stress) affected many mothers including older, larger mothers that tend to give birth to larger calves early in the season. Many tissue samples have been collected and analyzed, but no consistent lesions, pathologic processes or elevated levels of algal biotoxins have been identified to explain these recent mortality events. Here, we document the high mortality events, place them in historical context and describe ongoing efforts to identify their causes. As of 2010, the southern right whale sub-population that calves off Península Valdés was estimated to be less than 20% of its initial size before whaling; the ongoing high mortality of calves will significantly affect its recovery.","palabras_clave":"RIGHT WHALE |MORTALITY|EUBALAENA AUSTRALIS |STRANDINGS|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2718,"idioma_id":4,"titulo":"DETECTION OF ANTIBODIES AGAINST BOVINE HERPES VIRUS 1, BOVINE VIRAL DIARRHEA VIRUS AND BOVINE RESPIRATORY SYNCITIAL VIRUS IN EARLY AND ULTRA-EARLY WEANED BEEF CALVES","resumen":"Bovine respiratory disease is the leading cause of morbidity and mortality in weaned calves. In Argentina, two weaning practices have been implemented. In the early weaning, the calf is removed from the cow at 60-70 days of age while in ultra-early weaning the calf is weaned at 30-45 days of age. The purposes of both systems is to improve cow body condition, calf performance, conception rates and forage availability for the cow. In this study we evaluated the antibody response against BVDV and BoHV1 in early and ultraearly weaned calves that had received a conventional vaccination schedule (first dose at weaning and a booster 21 days post-weaning). Passively acquired immunity may provide protection against disease causedby these viruses. The presence of antibodies against BRSV, a virus that was not present in the vaccines used, was also evaluated as an indirect indicator of viral circulation in the herd. At the time of vaccination, calves presented a wide range of maternally-derived antibody titers. Vaccination against BoHV-1 did not evoke seroconvertion and antibody titers continued to decay throughout the experience. After vaccination, seroconversion to BVDV could be detected in calves with low antibody titers, while higher antibody titers exerted an inhibitory effect of the active humoral response.","palabras_clave":"Destete|Enfermedades respiratorias bovinas|Anticuerpos maternales|vacunas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2720,"idioma_id":5,"titulo":"Cambios de uso del suelo e impactos sobre el agua subterránea en un barrio al sur de Tandil, Buenos Aires, Argentina","resumen":"Este trabajo analiza los cambios de uso del suelo en el período 2003-2011 en un barrio de 79,62 ha ubicado al S interserrano de la ciudad de Tandil, y su vinculación con la calidad y disponibilidad de agua subterránea. El mapeo de imágenes satelitales y su contrastación en campo evidenciaron los cambios en los usos del suelo de la zona, pasando de lotes sin uso definido y cultivos a un incremento de aprovechamientos residenciales y turístico-comerciales principalmente. Debido a que el sector no es abastecido por servicios de agua de red y sistema cloacal, se utilizan perforaciones particulares para la extracción de agua del acuífero libre, para consumo humano y uso recreativo, y se depositan los efluentes domiciliarios en pozos absorbentes. Los habitantes informaron sobre problemáticas relacionadas al uso del agua como secado de pozos, necesidad de perforar a mayor profundidad, problemas de calidad del agua, escasez en época estival y para el llenado de piletas y colmatación de pozos ciegos. En 2008 y 2011 se midieron los niveles freáticos y se tomaron muestras de agua para conocer la conductividad eléctrica (CE) y el contenido de nitratos. El sentido de flujo subterráneo es hacia el N del área y se corroboraron descensos freáticos en 2011. La CE osciló entre 480 y 1400 µS\/cm denotando contaminaciones puntuales en la zona con mayor número de viviendas. El mismo comportamiento tuvieron los nitratos, variando desde 6 a 85 mg\/L, con un punto que supera 200 mg\/L. Las zonas con mayores concentraciones se ubican aguas abajo respecto al flujo subterráneo. Estas alteraciones están ocasionadas principalmente por la disposición in situ de los efluentes domiciliarios, que genera un ciclo semicerrado de disposición - transporte - extracción del agua. La falta de planificación territorial con visión ambiental, sumada a la ausencia de servicios sanitarios y la ocupación en cabeceras de cuenca, pone en peligro la calidad y disponibilidad de agua subterránea y en riesgo a la salud de quienes la consumen.","palabras_clave":"urbanización|planificación territorial|abastecimiento de agua subterránea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2722,"idioma_id":4,"titulo":"Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovarian syndrome","resumen":"      Numerous experimental models in different species have been developed for the study      24 of polycystic ovarian syndrome. In the present study we used a model of induction ofof polycystic ovarian syndrome. In the present study we used a model of induction of      25 polycystic ovaries in rats by exposure to constant light to study the distribution andpolycystic ovaries in rats by exposure to constant light to study the distribution and      26 variations of glycosylated residues present in the different ovarian structures. Sevenvariations of glycosylated residues present in the different ovarian structures. Seven      27 biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) onbiotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on      28 tissue sections and detection was performed using the streptavidine\/peroxidase method.tissue sections and detection was performed using the streptavidine\/peroxidase method.      29 In tissue sections was observed an increase in affinity for Con-A in the granulosa andIn tissue sections was observed an increase in affinity for Con-A in the granulosa and      30 theca interna of growing follicles and cysts in animals with polycystic ovaries intheca interna of growing follicles and cysts in animals with polycystic ovaries in      31 relation to the control group. Follicular cysts showed higher affinity for WGA andrelation to the control group. Follicular cysts showed higher affinity for WGA and      32 RCA-I which growing follicles in the same group and there was a decrease in affinityRCA-I which growing follicles in the same group and there was a decrease in affinity      33 for PNA in the cysts in relation to the growth of follicles in both groups. Atretic folliclesfor PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles      34 in both groups showed greater labeling with lectins PNA, SBA and RCA-I in relation toin both groups showed greater labeling with lectins PNA, SBA and RCA-I in relation to      35 healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost thehealthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the      36 affinity for the lectin Con-A. There was no staining on follicles in any category with theaffinity for the lectin Con-A. There was no staining on follicles in any category with the      37 lectins DBA and UEA-I, although it was staining in the corpus luteum (control group)lectins DBA and UEA-I, although it was staining in the corpus luteum (control group)      38 and in the mesothelium and interstitial glands of both groups with DBA. Theseand in the mesothelium and interstitial glands of both groups with DBA. These      39 observations probably reflect changes in the glycosaminoglycans present in the differentobservations probably reflect changes in the glycosaminoglycans present in the different      40 ovarian compartments or in the glycosylation of cellular components essential forovarian compartments or in the glycosylation of cellular components essential for      41 proper follicular dynamics.proper follicular dynamics.","palabras_clave":"Polycystic ovaries|Lectin binding pattern|Rats,|Glycoconjugates"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2723,"idioma_id":4,"titulo":"Effect of dietary calcium (Ca) on body composition and Ca metabolism during growth in genetically obese (&#946;) male rats.","resumen":"INTRODUCTION:Obese &#946; rats may be a suitable model to evaluate the association between calcium intake (CaI) and obesity during growth. OBJECTIVE:The present study comparatively evaluated Ca absorption and retention, and changes in body composition in spontaneously genetically obese (&#946;) male rats fed three different dietary Ca levels: high 0.9% (HCa); normal: 0.5% (NCa); low: 0.2% (LCa). METHODS:Pregnant rats were fed isocaloric diets which varied in Ca content only. Male pups continued feeding the same maternal diet until postnatal day 60. The percentage of Apparent Ca absorption (CaA %), Ca balance (CaB), body composition, glucose, triglycerides (TGL), and insulin levels were evaluated. RESULTS:Food consumption and body weight (BW) were higher in Group LCa than in Groups NCa and HCa (p CONCLUSIONS: Although further studies are required, low Ca consumption in this strain of rats could modulate BW inducing changes in several lipid metabolism parameters, which in turn lead to an increase in body fat.","palabras_clave":"Low calcium diet|Bone mass|Fat"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2724,"idioma_id":4,"titulo":"Effect of the number and location of watering point on animal activity on Digitaria eriantha pasture (Abstract)","resumen":"Daily distance traveled by grazing cattle is an indicator of animal activity according water and forage availability. 4187 geopositions from four cattle that alternately grazed 1, 2 or 3 watering point (WP) paddocks of Digitaria eriantha pastures were collected. All paddocks were similars, 400-450 ha and daily distance traveled of each animal was assessed from 15-09-2011 to 15-10-2011. After check the normality of data set and verify that there were not differences between animals, analysis of variance (ANOVA) and mean difference LSD-test were performed. Significant statistical differences (p <0.05) were found on daily distance traveled according number of WP, daily animal access to drinking and number of visited WP. On 1-WP paddocks the daily traveled distance is lower compared to 2-WP situations, since the animal the animal does not walk away from the WP. On 3-WP daily distance was not different from the others, probably by proximity and high number of WP in reduced area. No differences on daily distance traveled were found when 2 WP were located diagonally oposite or on the same fence. 24% of days animals did not drink water and walk a lower daily distance (4200 m vs. 6000 m, ± 230 m.) since not come from WP. We can infer that 2 WP on 450 has paddocks forced pasture exploration, while a third WP on this area has no effect on animal activity","palabras_clave":"CATTLE|GRAZING BEHABVIOUR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2725,"idioma_id":4,"titulo":"'A new species of Homonota (Reptilia: Squamata: Gekkota: Phyllodactylidae) endemic to the hills of Paraje Tres Cerros, Corrientes Province, Argentina'","resumen":"The genus Homonota comprises nine South American species of terrestrial and nocturnal lizards. Homonota lizards lack the  femoral pores typical of other South American Phyllodactylidae, and their infradigital lamellas are not expanded. We here  describe a new species, Homonota taragui sp. nov., exclusively found on a small group of three hills up to 179 meters above sea  level in central eastern Corrientes Province, Argentina. The new species differs from other Homonota species by a combination  of characters, including: a well-marked dorsal, reticulate, dark pattern contrasting with a lighter colored background; small,  star-shaped chromatophores on the abdomen; the post-orbital region of the head covered by granular scales; the dorsal and  anterior regions of the thighs covered by keeled scales interspersed with cycloid scales; and the internasal scale in contact with  rostral scales. The conservation status of Homonota taragui sp. nov. may be vulnerable, due to its localized endemism with  populations on three small hills surrounded by intense agricultural and livestock activity. Two endemic plant species are known  from these hills, and this new lizard represents the first endemic animal species.","palabras_clave":"HOMONOTA TARAGUI SP. NOV|SKY ISLAND|SMALL HILLS|TAXONOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2726,"idioma_id":4,"titulo":"Mid Holocene hunters and herders of Southern Cordillera (Northwestern Argentina)","resumen":"This paper explores the characteristics of human occupation in the area near the Argentine-Chilean international boundary, in the Andes cordillera (northwest of San Juan province). Characteristics of human occupation of these high altitude environments and changes in subsistence strategies that occurred during the Middle Holocene are evaluated. The study is based on the analysis of the materials at the site located in a seasonally environment rich in resources. The paper contributes to knowledge of the function and the variability of socio-cultural systems of the past in Andean environments in the southern part of the Northwest Argentina region (NOA). The problem is approached from studies of the organization of lithic technology, and osteometric analysis recovered at the ARQ-18 site. These studies indicate changes in subsistence and technological strategies of human groups that occupied ARQ-18, incorporating herding activities in a chronological range between 5100 and 4300 BP.","palabras_clave":"Mid-Holocene|Hunters and Herders|Southern cordillera|Northwest Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2776,"idioma_id":5,"titulo":"Acerca del escepticismo metódico cartesiano y su importancia para el desarrollo histórico del pensamiento filosófico. Consideraciones desde la perspectiva histórico-filosófica kantiana","resumen":"En este trabajo  se examina la posibilidad de interpretar el procedimiento de la duda metódica impulsado por Descartes como un antecedente del proyecto crítico kantiano. A través de un análisis de la reconstrucción que Kant propone, en diversos escritos, acerca del desarrollo histórico de la metafísica, intentaremos establecer los motivos de su valoración positiva de la instancia escéptica para el surgimiento y desarrollo del proyecto de una filosofía crítica (aclarando asimismo en qué sentido y hasta qué puntos las reflexiones metódicas cartesianas pueden ser consideradas como integradas en dicha instancia escéptica). Observaremos, finalmente, que tanto Descartes como Kant entienden el escepticismo como una estrategia metodológica eficaz en la búsqueda de una verdad objetiva, considerándolo incluso como un procedimiento esencial e indispensable de la razón filosófica.","palabras_clave":"DESCARTES|KANT|MÉTODO|ESCEPTICISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2777,"idioma_id":4,"titulo":".- Vanadium-substituted Wells-Dawson heteropolyacid as catalyst for liquid phase   oxidation of a 1,4-dihydropyridine derivative","resumen":"H7P2W17VO62.25H2O Wells-Dawson heteropolyacid was prepared, characterized and evaluated as catalyst in a 1,4-dihydropyridine oxidation reaction. The  optimal conditions are the following: 1 mmol% of HWDV, a molar ratio 1,4-DHP:H2O2 (1:10) at reflux of acetonitrile. A conversion of 98.6 % is Achieved in only 120 min of reaction.","palabras_clave":"Heteropolyacid |Oxidation|1,4-dihydropyridine|Hydrogen peroxide"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2785,"idioma_id":4,"titulo":"Prevalence of Pathogens Causing Subclinical Mastitis in Argentinean Dairy Herds","resumen":"The objective of this study was to investigate the prevalence of differentmastitis pathogens in Argentinean dairy farms. Composite milk samples were collectedof 2296 cows from 51 randomly selected herds in Córdoba, Argentina. Somaticcell count was determined in all samples and bacterial examination of the milksamples with a SCC exceeding 200,000 cells\/mL was performed. Of all the cows, 54%were diagnosed with subclinical mastitis (SCC &#8805;200,000\/mL). Bacteria wereisolated in 83.1% of milk samples subjected to bacteriological analysis. The mostfrequently isolated pathogen was coagulase-negative staphylococci (CNS) (52.1%),followed by Staphylococcus aureus(21.3%), Corynebacterium spp. (5.2%), Streptococcusagalactiae (4.4%) and Streptococcus dysgalactiae (4.4%). This study demonstratesthat among the major pathogens isolated, the contagious bacteria caused most ofthe subclinical infections of dairy cows in Cordoba, Argentina. Moreover, CNSwas the most relevant group of minor pathogens causing subclinical mastitis. ","palabras_clave":"BOVINE SUBCLINICAL MASTITIS |COAGULASE-NEGATIVE STAPHYLOCOCCUS |PREVALENCE|STAPHYLOCOCCUS AUREUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2792,"idioma_id":4,"titulo":"Assessment of different models for bathymetry calculation using SPOT multispectral images in a high-turbidity area: the mouth of the Guadiana Estuary","resumen":"Periodic calculation of coastal bathymetries can show the evolution of geomorphological features in active areas such as mesotidal estuary mouths. Bathymetries in shallow coastal areas have been addressed mainly by two technologies, lidar and optical remote sensing. Lidar provides good accuracy, but is an expensive technique, requiring planned flights for each region and dates of interest. Optical remote sensing acquires images periodically but its results are limited by water turbidity. Here we use a lidar bathymetry to compare different bathymetry computation methods using a SPOT optical image from a nearby date. Three statistical models (green-band, PCA correlations, and GLM) were applied to obtain mathematical expressions to estimate bathymetry from that image: all gave errors lower than 1 m in an area with depths ranging from 0 to 6 m. These algorithms were then applied to images from three different dates, correcting the effects caused by different tidal and atmospheric conditions. We show how this allows the study of morphological changes. We discuss the accuracy obtained with respect to the reference bathymetry (0.9 m on average, but less than 0.5 m in low-turbidity areas), the effects of the turbidity on our estimations, and compare both with previously published results. The results show that this approach is effective and allows identification of known features of coastal dynamics, and thus it would be an important step towards short-term bathymetry monitoring based on optical satellite remote sensing.","palabras_clave":"BATHYMETRY|REMOTE SENSING|SPOT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2793,"idioma_id":5,"titulo":"Jurisprudencia de la provincia de Mendoza 1\/2014","resumen":"Resumen de jurisprudencia relevante de la Suprema Corte de Justicia de Mendoza, relativa a materias de derecho administrativo, durante el cuarto trimestre del año 2014","palabras_clave":"derecho administrativo|jurisprudencia|Mendoza"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2794,"idioma_id":5,"titulo":"Autonomía y migración: los obreros forestales del nordeste de Misiones (Argentina)","resumen":"Este artículo versa sobre los peones forestales de la provincia de Misiones (nordeste de Argentina), que se desplazan hacia los bosques cultivados de Eucaliptos, situados en las provincias de Corrientes y Entre Ríos. Analizamos este circuito de migración cíclica en términos de segmentación del mercado de trabajo. La condición social de los migrantes se define por la alternancia entre la venta de fuerza de trabajo y el trabajo para sí en la parcela. La búsqueda de autonomía constituye un valor central en la selección de alternativas laborales. Desde la perspectiva de la antropología, describimos las formas de organización social (fases del ciclo doméstico, redes sociales) y las categorías culturales que intervienen en la segmentación.","palabras_clave":"MIGRACION LABORAL|MERCADO DE TRABAJO|EMPLEO FORESTAL|ANTROPOLOGIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2796,"idioma_id":4,"titulo":"Screening of Fusarium graminearum isolates for enzymes extracellular and deoxinivalenol production","resumen":"Fusarium graminearum, the main etiological agent of Fusarium Head Bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present report, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic and lipase extracellular enzymatic activities production, as well as by the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation with the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research, would allow us to apply a selection criterion among isolates for further research.","palabras_clave":"Extracellular enzimes|Deoxynivalenol|Fusarium graminearum|Wheat"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2797,"idioma_id":5,"titulo":"INFLUENCIA DE LOS EDIFICIOS EN ALTURA SOBRE ALGUNAS VARIABLES CLIMATICAS-2da parte","resumen":"En general cuando se estudian las consecuencias de las obras de infraestructura de gran magnitud realizadas por el hombre en un ambito urbano consolidado, se esta frente a modificaciones del clima urbano, el cual difiere con el del ambito suburbano debido a que aun la naturaleza no ha sido modificada marcadamente. Es por ello que es importante realizar estudios de impacto ambiental ante la posibilidad de la incorporacion de componentes antropogenicos significativos, no solo en areas urbanas sino tambien en la periferia de las ciudades.  En el caso particular de este estudio se ha trabajado sobre la influencia que estan teniendo en los ultimos tiempos las construcciones de edificios de gran altura y que en la mayoria de los casos no se efectua una evaluacion a conciencia del impacto ambiental que pudiera originar.  Nuestro objetivo fue analizar como se modificaban algunos parametros climaticos ante la presencia de obstaculos de gran magnitud como son las grandes edificaciones y que-ante la disminucion de tierras para construir viviendas-alcanzan cada vez mayores alturas.  Los parametros estadisticos que se han utilizados en este estudio en particular no son totalmente representativos del area analizada, ya que la estacion meteorologica que genera la informacion se encuentra aproximadamente a 6km de distancia y el no contar con datos dentro del area urbana nos obliga a recurrir a antecedentes de otras ciudades del pais y del extranjero.","palabras_clave":"CLIMA URBANO|EDIFICIOS EN TORRES|VARIABILIDAD DEL VIENTO, TEMPERATURA Y HUMEDAD|CONFORT AMBIENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2800,"idioma_id":4,"titulo":"Clinical profile of the association of p.R1205H and p.R924Q in a patient with von Willebrand disease (letter).","resumen":"The association of p.R924Q with a second mutation and with O blood group reinforces the decrease in FVIII:C and VWF. This could be the case in our patient, who showed moderate severe symptoms, but not in her mother, who only showed mild symptoms and the presence of p.R924Q associated with O blood group. In conclusion, we report here an association of VWD-type Vicenza with p.R924Q in a girl with a moderate\u2013severe bleeding phenotype, probably induced by the association of R924Q and O blood group. This is the first report of clinical description for this association.","palabras_clave":"VWD|Mutations|clinical profile"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2801,"idioma_id":4,"titulo":"Micropaleontological record of Holocene estuarine stages in the Bahía Blanca estuary, Argentina","resumen":"ABSTRACT. Holocene paleoenvironmental changes have been interpreted on the basis  of benthic foraminifera and calcareous nannofossils recovered in samples from Napostá  Grande Stream, Bahía Blanca estuary, southern Buenos Aires Province. Samples are  fine sands and clay sediments from a Holocene outcrop and were studied with  quantitative techniques. The benthic foraminiferal assemblage is dominated by  Ammonia parkinsoniana, A. tepida, Bolivina pseudoplicata, B. striatula, Bolivina sp.,  Buccella peruviana, and Elphidium spp. The calcareous nannofossil assemblage  recovered is a typical cold-water association, dominated by Calcidiscus leptoporus,  Coccolithus pelagicus, Emiliana huxleyi and Gephyrocapsa oceanica. A dendrogram  classification by cluster analysis was made for each microfossil group. The results of  these analyses were coincident, showing a liaison between changes in the assemblages  of benthic foraminifera and calcareous nannofossils. Those results, jointly with the  sedimentological information, lead to the identification of three different  paleoenvironments along the Napostá N1 site. The lower part of the succession  represents an estuarine environment with larger marine connection. The middle part  represents a gradual passage to a more restricted estuarine environment, and the upper  part represents the establishment of the modern continental fresh-water environment.","palabras_clave":"BENTHIC FORAMINIFERA|CALCAREOUS NANNOFOSSILS|ESTUARINE PALEOENVIRONMENTS|HOLOCENE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2802,"idioma_id":4,"titulo":"Early Gag Immunodominance of the HIV-Specific T-Cell Response during Acute\/Early Infection Is Associated with Higher CD8\u0001 T-Cell Antiviral Activity and Correlates with Preservation of the CD4\u0001 TCell Compartment","resumen":"The important role of the CD8 T-cell response on HIV control is well established. Moreover, the acute phase of infection representsa proper scenario to delineate the antiviral cellular functions that best correlate with control. Here, multiple functionalaspects (specificity, ex vivo viral inhibitory activity [VIA] and polyfunctionality) of the HIV-specific CD8 T-cell subset arisingearly after infection, and their association with disease progression markers, were examined. Blood samples from 44 subjectsrecruited within 6 months from infection (primary HIV infection [PHI] group), 16 chronically infected subjects, 11 elite controllers(EC), and 10 healthy donors were obtained. Results indicated that, although Nef dominated the anti-HIV response duringacute\/early infection, a higher proportion of early anti-Gag T cells correlated with delayed progression. Polyfunctional HIV-specificCD8 T cells were detected at early time points but did not associate with virus control. Conversely, higher CD4 T-cell setpoints were observed in PHI subjects with higher HIV-specific CD8 T-cell VIA at baseline. Importantly, VIA levels correlatedwith the magnitude of the anti-Gag cellular response. The advantage of Gag-specific cells may result from their enhanced abilityto mediate lysis of infected cells (evidenced by a higher capacity to degranulate and to mediate VIA) and to simultaneously produceIFN-. Finally, Gag immunodominance was associated with elevated plasma levels of interleukin 2 (IL-2) and macrophageinflammatory protein 1 (MIP-1). All together, this study underscores the importance of CD8 T-cell specificity in the improvedcontrol of disease progression, which was related to the capacity of Gag-specific cells to mediate both lytic and nonlyticantiviral mechanisms at early time points postinfection.","palabras_clave":"HIV|Cellular immune responses|protection|Gag"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2803,"idioma_id":4,"titulo":"Changes in the aerobic vaginal bacteria load and antimicrobial susceptibility after different oestrous synchronisation treatments in goats","resumen":"The objectives of this study were to determine (i) the variation in the   bacterial population, (ii) the changes in the number of aerobic   bacteria, and (iii) in vitro bacterial susceptibility to   different antibiotics after the use of two different intravaginal   devices (silicone devices or polyurethane sponges) for 6 or 11 days in   goats. During the breeding season, 25 multiparous Saanen goats were   randomly allocated to four treatments. Group IS-6 (n = 6) and Group IS-11 (n   = 6) goats received a polyurethane intravaginal sponge (60 mg   medroxyprogesterone acetate with oxytetracycline in powder) during 6 or   11 days, respectively. Goats from groups SD-6 (n = 7) and SD-11 (n   = 6) received an intravaginal silicone device (0.3 g progesterone)   during 6 or 11 days, respectively. Before the introduction of the   devices, at device withdrawal, and the day of oestrus we performed   standard bacteriological procedures on vaginal mucus samples. The   predominant bacterial flora at device insertion was gram-positive (77%),   and the most frequent strain was Bacilllus sp. The samples   obtained on Day 6 [SD-6: 9\/12 (75.0%); IS-6: 5\/9 (55.5%)] and Day 11   [SD-11: 3\/4 (75.0%); IS-11: 5\/5 (100%)] were predominantly   gram-negative, and the most frequently isolated bacteria was Arcanobacterium pyogenes.   The total number of aerobic bacteria forming colony units (CFU\/mL) was   greater in goats treated with inert silicon device that those treated   with intravaginal sponges (P = 0.028). On device removal (Days 6   and 11), we observed more CFU\/mL than on device insertion. We observed   no difference in CFU\/mL on day of oestrus with respect to the day of   device insertion (Day 0: 41.1 ± 19.3; Day 6: 187.1 ± 51.3; Day 11: 169.1   ± 27.8; day of oestrus: 20.1 ± 25.8 CFU × 103\/mL; P &lt; 0.05). The in vitro   studies demonstrated that most samples were resistant to   oxytetracycline. We concluded that the use of intravaginal devices,   regardless of their composition, provoked an increase in the normal   vaginal bacterial flora, but values returned to pre-insertion numbers by   the day of oestrus. According to in vitro results, amoxicillin, ampicillin and streptomycin were the most effective antibiotics to prevent vaginal bacterial growth.","palabras_clave":"antibiotic treatment,|mucous load, |oestrous synchronisation,| vaginal flora.| intravaginal devices,"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2804,"idioma_id":4,"titulo":"Effect of grain size on the forces governing the dynamics behavior of electrostatically driven powder media","resumen":"The aim of this paper is to identify the forces that govern the dynamic behavior of polydisperse granular systems under high electric fields in air media. In a previous paper, we reported the changes in the morphology and dynamics of grain structures under a frequency and amplitude variable voltage and summarized them in a diagram [L. M. Salvatierra et al., Chem. Phys. Lett. 481, 194 (2009)]. In the present article, we investigate the influence of the grain size distribution and the speed of voltage increase during tests. We explain the main transitions observed in the diagram in terms of the change in grain size and the frequency-dependent electrical response, related to electrophoretic and dielectrophoretic interactions.","palabras_clave":"POWDER|GRANULAR MATERIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2805,"idioma_id":4,"titulo":"Isomerization-cracking of n-octane on catalysts based on heteropolyacid H3PW12O40 and heteropolyacid supported over zirconia and promoted with Pt and Cs","resumen":"Isomerization?cracking of n-octane was studied using H3PW12O40 (HPA) and HPA supported on zirconia and promoted with Pt and Cs. The addition of Pt and Cs to the supported HPA did not modify the Keggin structure. The Pt addition to the supported HPA did not substantially modify the total acidity; however, the Brönsted acidity increased significantly. Cs increased the total acidity and Brönsted acidity. A linear relation was observed between the n-C8 total conversion and Brönsted acidity. The most adequate catalysts for performing isomerization and cracking to yield high research octane number (RON) are those with higher values of Brönsted acidity","palabras_clave":"REFORMULATED FUELS|SUPPORTED HETEROPOLYACIDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2806,"idioma_id":4,"titulo":"Early detection and quantification of mutations in the tyrosine kinase domain of chimerical BCR.ABL1 gene combining high-resolution melting analysis and mutant allele specific quantitative polymerase chain reaction","resumen":"Abstract  BCR-ABL1 point mutations are the most common cause of resistance in patients with chronic myeloid leukemia (CML) who fail or lose response to tyrosine kinase inhibitors. We have developed a rapid method to screen BCR-ABL1 mutations by high resolution melting (HRM). We designed a strategy based on amplification refractory mutational system-quantitative polymerase chain reaction (ARMS-qPCR) to identify and quantify several clinically relevant mutations. From 856 patients with CML studied during 2 years in our laboratory, we selected 32 who showed persistent levels of BCR-ABL1 transcripts (>0.1%) in at least two consecutive studies. Using our strategy, we identified mutations in 11\/32 cases (34.4%), while only two of them (6.2%) were detectable by sequencing. Furthermore, we were able to estimate the timing and dynamics of mutated clones, evaluating retrospective samples from the same patient. In cases with lack or loss of molecular response this analysis might be useful for designing early therapeutic strategies.","palabras_clave":"Chronic Myeloid Leukemia|BCR\/ABL1|Mutations|Inhibitors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2807,"idioma_id":4,"titulo":"Central insulin-Angiotensin II interaction in blood pressure regulation in fructose overloaded rats","resumen":"The aim of the present study was to determine if insulin is able to   modulate the pressor response to intracerebroventricularly administered   angiotensin II in insulin resistant fructose overloaded rats. Male Sprague-Dawley rats were divided into two groups: 1) Control group (C) with tap water to drink for 6weeks (n=36); and 2) fructose treated (F), with fructose solution (10% w\/v) to drink for 6weeks (n=36). On the day of the experiment, anesthetized male C and F rats   were intracerebroventricularly infused with insulin (12mU\/h, n=15) or   Ringer´s solution as vehicle (n=15) for 2h. Immediately, changes in mean   arterial pressure (MAP) in response to an intracerebroventricular subpressor dose of angiotensin II   (5pmol, n=10) or vehicle (n=5) were measured for 10min. Then,   hypothalami were removed and Akt and ERK1\/2 phosphorylation levels were   determined. In a subset of C (n=10) and F (n=20) animals, PD98059   (p44\/42 MAPK inhibitor) or vehicle was administered   intracerebroventricularly at a flow rate of 5&#956;l\/min for 1min. Ten   minutes later, insulin (12mU\/h, n=5 for each group) or vehicle (Ringer´s   solution, only in the F group, n=5) was perfused for 2h at a flow rate   of 4&#956;l\/h, and cardiovascular parameters were measured every 15min.   Immediately, changes in MAP and HR in response to a subpressor dose of   Ang II   (5pmol\/2&#956;l) were evaluated for 10min (n=5 for each group). In other   subset of animals (n=6 for each group), AT1 and AT2 hypothalamic   receptor levels were measured by Western blotting.   Intracerebroventricular insulin pre-treatment increased the pressor   response to angiotensin II in C rats. In F rats (with or without insulin pretreatment), the pressor response to angiotensin II was higher than that in vehicle pre-treated C animals, but similar to that observed in C after insulin infusion. In C rats phospho-ERK 1\/2 hypothalamic levels significantly increased after angiotensin II injection in insulin pretreated animals compared to vehicle pre-treated rats, suggesting that MAPK activation might be involved in insulin potentiation of blood pressure response to angiotensin II in the brain. Phospho-ERK 1\/2 hypothalamic levels were significantly increased in vehicle treated F rats compared to C, suggesting that basal MAPK activation might play a role in the enhanced response to angiotensin II observed in these animals. Finally, in F rats, either after vehicle or insulin infusion, angiotensin II   injection was associated with a similar increase in phospho-ERK 1\/2   hypothalamic levels, comparable to that observed after angiotensin II injection in insulin pre-treated C animals. ERK 1\/2 blockade significantly reduced MAP in F rats compared to C. Moreover, ERK 1\/2 inhibition completely abolished the Ang II pressor response in F rats and in insulin pre-treated C animals. All these findings suggest that insulin-angiotensin II interaction at hypothalamic level might be involved in the increase in blood pressure observed in the insulin resistant state.","palabras_clave":"INSULIN|ANGIOTENSIN II|FRUCTOSE|HYPERTENSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2809,"idioma_id":4,"titulo":"Evaluation of the cryotech vitrification kit for bovine embryos","resumen":"The purpose of this work was to assess commercially available Cryotech Vitrification Kit, in terms of survival, in vitro development and pregnancy rate for bovine embryos. Cumulus-oocyte complexes (COCs) were recovered from ovaries obtained from slaughtered cows and then matured in vitro for 22 h. COCs were fertilized by sex-sorted sperm in IVF-mSOF and cultured in IVC-mSOF for 7 days to the blastocyst stage. Blastocysts were vitrified with the Cryotech Vitrification Kit® and then either warmed to check viability or transferred to synchronized heifers. We observed 100% survival of the in vitro produced blastocysts and obtained the same pregnancy rate (46.8%) as that obtained using fresh in vitro produced blastocysts. We thus conclude that the Cryotech vitrification method is a valid alternative to other vitrification or slow cooling methods in the bovine species and that it is ready for livestock production. ","palabras_clave":"Bovine embryo vitrification|Bovine embryo IVP|Cryotech|Vitrification"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2811,"idioma_id":4,"titulo":"Pod production in a 'caldén' forest that suffered antropogenic disturbances.(Abstract)","resumen":"Caldén pods constitute a forage contribution to the diet of cattle   beeding systems on natural grasslands. A stationary forest with   300 caldenes\/ ha produces 600kg DM\/ha. The aim was to   evaluate the production of pods in a forest that suffered   anthropogenic disturbances: selective logging about 50 years   ago and recently fire. Pod production was done for 132 trees of   different sizes according to trunk diameters. Four samples of 1   m  2 were collected from each tree, and average weight was   determined. Based on canopy cover DMpods\/tree was   calculated. The aim was to estimate the number of trees per   hectare the method of Cottan and Curtis (1956) was applied,   based on the average distance between trees. The number of   trees\/ha and the relative density of trunk diameters was arranged   in class intervals. The total production of pods\/ha was calculated   as a product between average annual production\/tree and the   number of trees\/ha for each class interval. According to the   relative tree density, the shape of the population pyramid   resembles two pyramids, one upon the other, coinciding the   union with the effect of logging. Total production was 195 kg   of DM\/ha, provided by the 47% of the trees (Total of 300),   which corresponded to a diameter of more than 0.20 m. Weather   conditions were favourable for flowering and fruiting, so the   lower production is estimated to be the result of the forest   structure resulting from the disturbances suffered over time","palabras_clave":"Prosopis caldenia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2813,"idioma_id":5,"titulo":"Anatomía comparada de Vertebrados. Actividades para el estudio de los órganos respiratorios","resumen":"En las prácticas se estudia el sistema nerviosos de los vertebrados. Se aprende la técnica de disección del encéfalo. Se estudia la anatomía comparada de las diferentes regiones del encéfalo y la relación con los órganos de los sentidos.","palabras_clave":"NERVIOSO|TÉCNICAS|PERIFÉRICO|AUTÓNOMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2814,"idioma_id":5,"titulo":"Lesión de Células Gigantes Central de los Maxilares. Reporte de un caso y revisión de la  Literatura.","resumen":"Lesión central de células gigantes (LCCG) es una patología osteolítica localizada generalmente en los maxilares. Su patogenia es controversial pero se cree se origina tras un traumatismo. Se manifiesta como un nódulo rojo-púrpura, de crecimiento lento y constituido por células gigantes multinucleadas inmersas en un estroma de células ovaladas y fusiformes, planteando diagnósticos diferenciales con otras lesiones a células gigantes de maxilares. A pesar de su histología benigna esta lesión suele ser agresiva localmente con tendencia a recidiva. Nuestros objetivos fueron reflejar la frecuencia de LCCG en el Servicio de Anatomía Patológica de Facultad de Odontología en un período de 23 años, indicar la importancia clínico-patológica, plantear diagnósticos diferenciales y mostrar un caso clínico. El mismo está representado por un paciente masculino de 27 años, con una lesión única osteolítica en el maxilar superior, con antecedente de traumatismo. Clínicamente la lesión era multilobulada de color rojo vinoso en encía vestibular antero-superior. El diagnóstico por imágenes mostró un comportamiento invasivo. La histopatología evidenció células gigantes multinucleadas, estroma fibrocelular vascularizado con áreas hemorrágicas y hemosiderina, llegando al diagnóstico de Lesión Central de Células Gigantes. En este caso la edad, localización e histopatología se correspondieron con la bibliografía consultada. En nuestro servicio la frecuencia de LCCG fue del 0,002% con una relación 3=1 mujer\/ hombre y con una edad promedio de aparición a los 30 años. Concluimos en la importancia del correcto diagnostico clínico-patológico de LCCG, que obliga a plantear diagnósticos diferenciales con patologías a células gigantes de maxilares para su adecuado tratamiento","palabras_clave":"CELULAS GIGANTES|TRAUMATISMO|LESION OSTEOLITICA|MAXILARES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2883,"idioma_id":4,"titulo":"Fast synthesis of a tris(N,N-diimine)chromium(III) complex by a microwave-assisted approach","resumen":"Background: In the last ten years, Photodynamic Antimicrobial Chemotherapy (PACT)  has emerged as a reliable option to threat several bacterial and fungal infections. Tris(N,Ndiimine)  chromium(III) complexes are known for their potential as photosensitizers;  however, synthesis is usually a very time consuming procedure. Results: With our novel  methodology, [Cr(biim)3](NO3)3 was prepared in a few seconds as a powder and the yield  of the reaction was improved compared with previously described synthesis, where  obtaining crystals is complicated due to the acidity of the media. The precursors for the  synthesis are also synthesized through fast and simple methods. Identification of  intermediates and the final product was performed by melting point, infrared, UV-vis and  NMR spectroscopy. Conclusions: This article describe a quick, easy and eco-friendly  procedure to synthesize the [Cr(biim)3](NO3)3 employing microwave irradiation.","palabras_clave":"SYNTESIS|MICROONDAS|CROMO|FOTOSENSITIZADOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2885,"idioma_id":4,"titulo":"Functional traits enhance invasiveness of bamboos over co-occurring tree saplings in the semideciduous Atlantic Forest","resumen":"Many woody bamboo species are forest understory plants that become invasive after disturbance. Theycan grow rapidly forming a dense, nearly monospecific understory that inhibits tree regeneration. Theprincipal aim of this study was to understand what functional traits of bamboos allow them tooutcompete tree seedlings and saplings and become successful species in the semideciduous AtlanticForests of northeastern Argentina. We studied leaf and whole-plant functional traits of two bamboospecies of the genus Chusquea and five co-occurring saplings of common tree species growing undersimilar solar radiation and soil nutrient availabilities. Nutrient addition had no effect on bamboo or treesapling survival and growth after two years. Tree species with high-light requirements had highergrowth rates and developed relatively thin leaves with high photosynthetic capacity per unit leaf areaand short leaf life-span when growing in gaps, but had lower survival rates in the understory. Theopposite pattern was observed in shade-tolerant species that were able to survive in the understory buthad lower photosynthetic capacity and growth than light-requiring species in gaps. Bamboos exhibited ahigh plasticity in functional traits and leaf characteristics that enabled them to grow rapidly in gaps (e.g.,higher photosynthetic capacity per unit dry mass and clonal reproduction in gaps than in the understory) but at the same time to tolerate closed-canopy conditions (they had thinner leaves and a relatively longer leaf life-span in the understory compared to gaps). Photosynthetic capacity per unit dry mass was higher in bamboos than in trees. Bamboo plasticity in key functional traits, such as clonal reproduction at the plant level and leaves with a relatively low C cost and high photosynthesis rates, allows them to colonize disturbed forests with consequences at the community and ecosystem levels. Increasing disturbance in some forests worldwide will likely enhance bamboo invasion resulting in profound negative impacts on forest diversity, structure and function in the long term.","palabras_clave":"ver  pdf."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2887,"idioma_id":4,"titulo":"Hyperhalophilic archaeal biofilms: growth kinetics, structure and antagonistic interaction in continuous culture","resumen":"                    Biofilms by the  hyperhalophilic archaea Halorubrum  sp. and Halobacterium sp. were  analyzed and for the first time the progression of structural features and  developmental parameters of these sessile populations was described. Optical  slicing and digital analysis of sequential micrographs showed that their 3D  structure was microorganism dependent. Biofilms of Halobacterium sp. developed in clusters that covered about 30% of  the supporting surface at the interface level and expanded over about 86±4 µm in thickness, while  Halorubrum sp. biofilms covered less than 20% of the surface and reached a  thickness of 41±1 µm. The kinetics of growth was lower in biofilms,  with generation times of 27±1 and 36±2 h for Halobacterium sp. and Halorubrum sp., respectively, as compared to those of 8.4±0.3 and 14±1 h in planktonic cultures. Differences between organisms were also  observed at the cell morphology level.                     The interaction between  the two microorganisms was also evaluated, showing that Halobacterium sp. can outcompete already established Halorubrum sp. biofilms by a mechanism  that might include the combined action of tunneling swimmers and antimicrobial  compounds.   ","palabras_clave":"ANTAGONISTIC INTERACTIONS|ARCHAEA|BIOFILMS|HALOPHILES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2888,"idioma_id":5,"titulo":"Adaptabilidad y estabilidad de variedades de sojaen la región II-2 Argentina. Campañas 2009\/10, 2010\/11 y 2012\/13,","resumen":"Evaluación de la estabilidad y adaptabilidad de variedades de soja en el centro de Santa Fe.","palabras_clave":"Soja|estabilidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2889,"idioma_id":5,"titulo":"Fisonomía del transporte urbano argentino. Del ?auto colectivo´ a los ómnibus ar-ticulados. Primera parte","resumen":"Este artículo plantea el análisis del transporte urbano en Argentina desde sus inicios hasta la actualidad, a través de las características tipológicas de sus vehículos. Conocer sus rasgos tecnológicos, estéticos, funcionales, ergonómicos   permite también decodificar modos de hacer, de pensar y de sentir que establecen el patrimonio de un pueblo.    El artículo en su primera parte abordó la evolución de los vehículos utilizados en el transporte urbano en Argentina desde 1928 y hasta mediados de los ´60, desde la perspectiva del diseño. En esta segunda parte se examina la situación desde esa última época y hasta la actualidad, describiéndose en ella las características morfológicas de los principales tipos de ómnibus, trolebuses y colectivos aplicados en el transporte urbano nacional.","palabras_clave":"TRANSPORTE URBANO|VEHÍCULOS|DISEÑO VEHICULAR|TRANSPORTE PÚBLICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2890,"idioma_id":5,"titulo":"LA DECLINACIÓN DEL PADRE EN LA ENSEÑANZA DE LACAN (1938-1961)","resumen":"En el presente trabajoabordamos la problemática de la declinación del padre en la enseñanza de Lacan,desde su texto sobre ?Los complejos familiares en la formación del individuo? de1938, hasta su lectura de la trilogía de P. Claudel en 1961. Destacamos cómoesta elaboración antecede la primera pluralización de los nombres del padre queefectúa en 1963, constituyendo un antecedente fundamental para abordar laproblemática de las versiones del padre en la última parte de suenseñanza.&nbsp; ","palabras_clave":"PADRE|DECLIVE|LACAN|PLURALIZACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2891,"idioma_id":4,"titulo":"Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)","resumen":"Abstract Genus Helianthus comprises diploid and polyploidspecies. An autoallopolyploid origin has been proposedfor hexaploid species but the genomic relationships remainunclear. Mitotic and meiotic studies in annual Helianthusannuus (2n = 2x = 34) and perennial Helianthus resinosus(2n = 6x = 102) as well as the F1 hybrids between bothspecies were carried out. Chromosome counting confirmedthe hybrid origin of the latter plants and their tetraploidcondition. Bivalents in hybrids ranged from 12 to 28(x = 20.8). Univalents, trivalents and quadrivalents werealso observed. Meiotic products comprised dyads, triads andnormal tetrads and pollen grains were heterogeneous in size.These observations suggest the occurrence of 2n pollen inaddition to the expected n. Genomic in situ hybridization(GISH) of total H. annuus DNA on H. resinosus chromosomesrendered weak but uniform signals; similar hybridizationpattern was observed using three other annualspecies. Hybridization with H. annuus probe performed onroot tip cells of F1 H. annuus9H. resinosus hybrids revealed17 chromosomes with a strong hybridization signal. GISH inhybrid meiocytes distinguished chromosomes from parentalspecies and revealed autosyndetic pairing of H. resinosuschromosomes, allosyndetic pairing in bivalents, trivalentsand quadrivalents, and the presence of univalents derivedfrom parents, H. annuus and H. resinosus. Results obtainedfrom classical and molecular cytogenetics do not support H.annuus as a direct ancestor of H. resinosus. The occurrenceof allosyndetic pairing and the relatively high fertility of theF1 hybrids point to the possibility that useful genes could betransferred from H. resinosus to cultivate sunflower,although the effective rate of recombination has not beenevaluated. GISH method proved effective to recognizeparental chromosomes in H. annuus9H. resinosus progeny.","palabras_clave":"2n pollen|Allosyndesis|GISH|Interspecific hybrid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2893,"idioma_id":4,"titulo":"Behaviourally related neural plasticity in the arthropod optic lobes","resumen":"BACKGROUND:   Due to the complexity and variability of natural   environments, the ability to adaptively modify behavior is of   fundamental biological importance. Motion vision provides essential cues   for guiding critical behaviors such as prey, predator, or mate   detection. However, when confronted with the repeated sight of a moving   object that turns out to be irrelevant, most animals will learn to   ignore it. The neural mechanisms by which moving objects can be ignored   are unknown. Although many arthropods exhibit behavioral adaptation to   repetitive moving objects, the underlying neural mechanisms have been   difficult to study, due to the difficulty of recording activity from the   small columnar neurons in peripheral motion detection circuits.  RESULTS:   We   developed an experimental approach in an arthropod to record the   calcium responses of visual neurons in&nbsp;vivo. We show that peripheral   columnar neurons that convey visual information into the second optic   neuropil persist in responding to the repeated presentation of an   innocuous moving object. However, activity in the columnar neurons that   convey the visual information from the second to the third optic   neuropil is suppressed during high-frequency stimulus repetitions. In   accordance with the animal´s behavioral changes, the suppression of   neural activity is fast but short lasting and restricted to the retina´s   trained area.  CONCLUSIONS:   Columnar neurons from the   second optic neuropil are likely the main plastic locus responsible for   the modifications in animal behavior when confronted with rapidly   repeated object motion. Our results demonstrate that visually guided   behaviors can be determined by neural plasticity that occurs   surprisingly early in the visual pathway.","palabras_clave":"Motion vision|arthropod|lobula|calcuim imaging"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2894,"idioma_id":4,"titulo":"Design, Synthesis and Biological Evaluation of WC-9 Analogues as Antiparasitic Agents","resumen":"As a part of our project pointed at the search of new safe chemotherapeutic and chemoprophylactic agents against parasitic diseases, several compounds structurally related to 4- phenoxyphenoxyethyl thiocyanate (WC-9), which were modified at the terminal aromatic ring, were designed, synthesized and evaluated as antiproliferative agents against Trypanosoma cruzi, the parasite responsible of American trypanosomiasis (Chagas disease) and Toxoplasma gondii, the etiological agent of toxoplasmosis. Most of the synthetic analogues exhibited similar antiparasitic activity being slightly more potent than the reference compound WC-9. For example, the nitro derivative 13 showed an ED50 value of 5.2 mM. Interestingly, the regioisomer of WC-9, compound 36 showed similar inhibitory action than WC-9 indicating that para-phenyl substitution pattern is not necessarily required for biological activity. The biological evaluation against T. gondii was also very promising. The ED50 values corresponding for 13, 36 and 37 were at the very low micromolar level against tachyzoites of T. gondii.","palabras_clave":"Antiparasitic agents|Chagas disease| Squalene synthase inhibitors|Toxoplasma gondii WC-9 analogs|Toxoplasmosis|Trypanosoma cruzi"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2895,"idioma_id":4,"titulo":"Brassica napus hairy roots and rhizobacteria for phenolic compounds removal","resumen":"Phenolic compounds are contaminants frequently found in water and soils. In the last years, some technologies such as phytoremediation have emerged to remediate contaminated sites. Plants alone are unable to completely degrade some pollutants; therefore their association with rhizospheric bacteria has been proposed to increase phytoremediation potential, an approach called rhizoremediation. In this work, the ability of two rhizobacteria, Burkholderia kururiensis KP 23 and Agrobacterium rhizogenes LBA 9402, to tolerate and degrade phenolic compounds was evaluated. Both microorganisms were capable to tolerate high concentrations of phenol, 2,4-dichlorophenol (2,4-DCP), guaiacol or pentachlorophenol (PCP); and degrade different concentrations of phenol and 2,4-DCP. Association of these bacterial strains with B. napus hairy roots, as model plant system, showed that the presence of both rhizospheric microorganisms, along with B. napus hairy roots, enhanced phenol degradation compared to B. napus hairy roots alone. These findings are interesting for future applications of these strains in phenol rhizoremediation processes, with whole plants, providing an efficient, economic, and sustainable remediation technology.","palabras_clave":"Removal|Rhizobacteria|Phenol|2,4-DCP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2896,"idioma_id":4,"titulo":"-Preliminary zooplancton study of six ?merceditas? lakes (San Luis, Argentina).","resumen":"The south of the province of San Luis (Argentina) has more than 100 lakes located among dunes and grasslands, known as 'mercedinas' due to their proximity to the city of Villa Mercedes. Although there are several reports on the ecology of zooplankton in other regions of the country, very little is known about the aquatic ecosystems of this extensive area. The aim of the study was to determine the taxonomic composition and the zooplankton density of six ?mercedinas? lakes and analyze the relationship between this community and limnological parameters and fish fauna. The samplings were carried out in October 2007. The average depth of the six lakes ranged between 2.3 and 3.6 m. Salinity ranged between 1.3 and 3.3 g.L-1, so they can be classified as subsaline to slightly hyposaline. Water transparency varied widely (0.3-2.3 m). Macrophytes were present in the six lakes and fish richness was represented by seven species, with predominance of Jenynsia multidentata and Odontesthes bonariensis. Zooplankton richness included 20 taxa: five cladocerans, six copepods and nine rotifers. The species common to the six lakes were Bosmina huaronensis, Ceriodaphnia dubia and Filinia longiseta. We found a situation that does not match that proposed by the model of alternative states of shallow lakes: the lakes where we did not find O. bonariensis, a main predator of zooplankton, showed the lowest water transparency, although a large filtering cladoceran such as Daphnia spinulata was present in one of them. These preliminary results make it necessary to carry out further research to see if this situation is maintained over time or if this was a specific situation","palabras_clave":"ZOOPLANKTON|SHALLOW LAKES|BOSMINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2897,"idioma_id":4,"titulo":"Mass spectral studies of diamide compounds obtained by the Ugi reaction","resumen":"La revista no dispone abstract","palabras_clave":"Diamides|Mass Spectrometry|Ugi Reaction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2898,"idioma_id":4,"titulo":"Structural aspects of PtSn\/g-Al2O3 catalysts prepared through surface controlled reactions. Behavior in the water denitrification reaction","resumen":"This research focuses on the controlled preparation and characterization of PtSn\/-Al2O3 catalysts andtheir activity and selectivity in water denitrification by reduction of NO3&#8722; and NO2&#8722; ions with hydrogen.The bimetallic PtSn catalysts were active in the hydrogenation of both NO3&#8722; and NO2&#8722; and the amount ofSn added had a pronounced influence on the activity of the catalysts, being PtSn0.25 catalyst the one withthe best performance. An explanation for this behavior is given in terms of the electronic and geometriceffects that the addition of tin exerts on Pt.","palabras_clave":"nitrates|nitrites|pt|surface-controlled"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2899,"idioma_id":5,"titulo":"Anatomía comparada de Vertebrados. Actividades para el estudio del sistema circulatorio","resumen":"En las prácticas se estudia el tegumento de los Vertebrados. Se realiza un adiestramiento en las técnicas que faciliten la observación de las estructuras. Se identifican los derivados de origen epidérmico, dérmico o mixto.","palabras_clave":"TEGUMENTO|TÉCNICAS|DERIVADOS|VERTEBRADOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2901,"idioma_id":10,"titulo":"Measurement of angular correlations in Drell-Yan lepton pairs to probe Z\/gamma* boson transverse momentum at sqrt(s)=7 TeV with the ATLAS detector","resumen":"A measurement of angular correlations in Drell-Yan lepton pairs via the @f_@h^@? observable is presented. This variable probes the same physics as the Z\/@c^@? boson transverse momentum with a better experimental resolution. The Z\/@c^@?-&gt;e^+e^- and Z\/@c^@?-&gt;@m^+@m^- decays produced in proton-proton collisions at a centre-of-mass energy of s=7 TeV are used. The data were collected with the ATLAS detector at the LHC and correspond to an integrated luminosity of 4.6 fb^-^1. Normalised differential cross sections as a function of @f_@h^@? are measured separately for electron and muon decay channels. These channels are then combined for improved accuracy. The cross section is also measured double differentially as a function of @f_@h^@? for three independent bins of the Z boson rapidity. The results are compared to QCD calculations and to predictions from different Monte Carlo event generators. The data are reasonably well described, in all measured Z boson rapidity regions, by resummed QCD predictions combined with fixed-order perturbative QCD calculations or by some Monte Carlo event generators. The measurement precision is typically better by one order of magnitude than present theoretical uncertainties.A measurement of angular correlations in Drell-Yan lepton pairs via the phistar observable is presented. This variable probes the same physics as the Z\/gamma* boson transverse momentum with a better experimental resolution. The Z\/gamma*-&gt;e+e- and Z\/gamma*-&gt;mu+mu- decays produced in proton--proton collisions at a centre-of-mass energy of sqrt(s) = 7 TeV are used. The data were collected with the ATLAS detector at the LHC and correspond to an integrated luminosity of 4.6 fb-1. Normalised differential cross sections as a function of phistar are measured separately for electron and muon decay channels. These channels are then combined for improved accuracy. The cross section is also measured double differentially as a function of phistar for three independent bins of the Z boson rapidity. The results are compared to QCD calculations and to predictions from different Monte Carlo event generators. The data are reasonably well described, in all measured Z boson rapidity regions, by resummed QCD predictions combined with fixed-order perturbative QCD calculations. Some of the Monte Carlo event generators are also able to describe the data. The measurement precision is typically better by one order of magnitude than present theoretical uncertainties.","palabras_clave":"ATLAS|high|energy|physics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2904,"idioma_id":4,"titulo":"Search for light top squark pair production in final states with leptons and b&#8722; jets with the ATLAS detector in s&#8730;=7 TeV proton-proton collisions","resumen":"The results of a search for pair production of light top squarks are presented, using 4.7 fb^-1 of sqrt(s) = 7 TeV proton-proton collisions collected with the ATLAS detector at the Large Hadron Collider. This search targets top squarks with masses similar to, or lighter than, the top quark mass. Final states containing exclusively one or two leptons (e, mu), large missing transverse momentum, light-jets and b-jets are used to reconstruct the top squark pair system. Global mass scale variables are used to separate the signal from a large ttbar background. No excess over the Standard Model expectations is found. The results are interpreted in the framework of the Minimal Supersymmetric Standard Model, assuming the top squark decays exclusively to a chargino and a b-quark. Light top squarks with masses between 123-167 GeV are excluded for neutralino masses around 55 GeV.","palabras_clave":"ATLAS|CERN|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2975,"idioma_id":5,"titulo":"Confirmación de la presencia de Phrynops geoffroanus (Schweigger, 1812) (Testudines, Chelidae) en la provincia de Misiones, Argentina. Cuadernos de Herpetología","resumen":"Phrynops geoffroanus (Schweigger,1812). República Argentina provincia de Misiones,departamento Capital (27° 21? 48.59? S; 55° 56? 30.71? O, Fig. 1). Colectores Rodrigo Cajade, Federico Marangoni y José Miguel Piñeiro. El día 13 de Julio de 2013 a las 16:00 hs se colectó un individuo adulto, que fue depositado en la Colección Herpetológica del Laboratorio de Genética Evolutiva (LGE 6420),Facultad de Ciencias Exactas, Químicas y Naturales,  Universidad Nacional de Misiones.","palabras_clave":"Phrynops geoffroanus|Confirmación|Posadas|Misiones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2976,"idioma_id":4,"titulo":"New data on the distri bution and  natural history  of the lesser grison ( Galictis cuja ),  hog-nosed  skunk ( Conepatus chinga  ), and culpeo (  Lycalopex  culpaeus  )  in  northwestern  Argentina","resumen":"We present a total of 190 new distribution records   of three little-known mammalian carnivores ( Conepatus   chinga  ,    Galictis cuja , and   Lycalopex culpaeus )  obtained   using camera trap techniques and direct observation in   the highlands of Jujuy province, northwestern Argentina.   These new records extend the present known distribu-tions of these three species to the west of the province and   to higher altitudes, and provide additional information on   habitat association and activity patterns.","palabras_clave":"Huron|Zorro colorado|zorrino"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2977,"idioma_id":4,"titulo":"Applying Health Risk Analysis to Assess the Chemical Quality of Water for Recreational Bathing: Case of Tres Arroyos Creek, Buenos Aires, Argentina","resumen":"            The aim of the study is to assess the probabilistic non-cancer and cancer risks by recreational bathing in Tres Arroyos creeks (southeastern Buenos Aires Province, Argentina). In these waters, hazardous substances (heavy metals, pesticides) have been detected, possibly related to agricultural activities. To assess such risk, USEPA models in aggregated (exposure through accidental oral water intake and dermal contact&nbsp; simultaneously) and cumulative approaches (combined exposure to more than one substance) were applied, performed for bathers of 5, 10, 15, and 20 years old. The results show that chronic bathing in these waters is not harmful at the concentrations and the exposure scenarios considered. Arsenic was the riskiest substance for both non-cancer and cancer effects, affecting mainly the youngest age group, and the accidental water intake during bathing was the most relevant pathway of exposure. On the other hand, the study highlights the key role of the frequency and duration of the bath event. We discuss the results in light of a previous paper of our authorship concluding that the health risk&nbsp; ssessment is a valid alternative to analyze recreational water quality, which, unfortunately, is unused by wáter management agencies in Argentina.","palabras_clave":"Agua recreativa|contaminación|risgo a la salud|Metales pesados, agroquimicos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2979,"idioma_id":5,"titulo":"?\tEfecto del alendronato sobre el perfil de citoquinas y metaloproteinasas 2 y 9 en un modelo múrido de artritis experimental","resumen":"ABSTRACT Introduction  Arthritis is one of the most common arthropathies, characterized by cartilage damage associated with subchondral bone resorption. Early diagnosis is crucial for the prevention of subchondral bone lesions.    Objectives  To use an experimental adjuvant arthritis rat model, to measure joint biomarkers, interleukins (IL-4, IL-6 and TNF-&#945;), and metalloproteases (MMP-2 and MMP-9) to follow the progression of the disease and to analyze the possible changes in the different treatment groups, with preventive or curative alendronate,    Materials and methods  Preventive alendronate was applied on day 0, and a curative regimen 2 months post-adjuvant. A clinical scale score was used for characterizing clinical symptoms, and, at sacrifice, joint biomarkers and histopathological and radiographic studies were determined.    Results  The most notable result was that the group that received preventive alendronate reached a normal clinical score faster than control adjuvant group. All alendronate groups showed significantly lower MMPs levels.    Conclusions  Alendronate apparently decreases proteases activity linked to the pathophysiology of joint disease, this could be extremely beneficial for the clinical outcome of arthritis.","palabras_clave":"ALENDRONATO|OSTEOARTRITIS|CITOQUINAS|METALOPROTEINASAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2981,"idioma_id":4,"titulo":"Biometric identification of capillariid eggs from archaeological sites in Patagonia","resumen":"Numerous eggs of capillariid nematodes have been found in coprolites from  a wide range of hosts and in raptor pellets in archaeological samples from Patagonia. The structure and sculpture of the eggshell of these nematodes and their biometry are commonly used for identification. The aim of this study was to determine whether eggs of the genus Calodium with similar morphology, found in different archaeological samples from Patagonia, belong to the same species. For this purpose, capillariid eggs (N = 843) with thick walls and radial striations were studied by permutational multivariate analysis of variance(PERMANOVA). Eggs exhibiting similar shape and structure also showed similar biometry, regardless of the zoological origin of coprolites (P = 0.84), host diet (P = 0.19), character of the archaeological sites (P = 0.67) and chronology (P = 0.66). Thus, they were attributed to the same species. We suggest that an unidentified zoonotic species of the genus Calodium occurred in the digestive tract of a wide range of hosts in Patagonia during the Holocene and that both human and animal populations were exposed to this parasite during the Holocene in the study area.","palabras_clave":"CAPILLARIID|PALEOPARASITOLOGY|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2982,"idioma_id":4,"titulo":"Symmetry Issues in Mixed Integer Programming Based Unit Commitment","resumen":"A mixed integer linear program is called symmetric if its variables can be permuted without changing the structure of the problem. Generally, these programs are more difficult to solve using enumerative algorithms due to isomorphic subproblems with redundant solutions populating the enumeration tree. Symmetry arises in Unit Commitment due to scheduling on identical machines. This work presents a way to reduce computational burden by adding appropriate inequalities to the mixed-linear formulation of Thermal Unit Commitment. In the examples considered, this approach leads to an impressive reduction of search and consequently, lesser computational times, without affecting the objective value.","palabras_clave":"Mixed integer linear programing|symmetry|unit commitment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2983,"idioma_id":5,"titulo":"LAS TRANSFORMACIONES SOCIALES EN LA POSCONVERTIBILIDAD","resumen":"El objetivo de este trabajo es profundizaren algunos aspectos clave de la transformación de la estructura social operadaen la primera década de nuestro siglo.En este sentido, referiremos a lascaracterísticas de los sectores dominantesa través del análisis de sucomportamiento en la industria y las actividades extractivas. Asimismo se darácuenta de las clases medias, sus atributos generales y las discusiones sobre suexpansión en el último tiempo y de las transformaciones ocurridas en el mercadode trabajo y las características de la pobreza y la indigencia. Finalmente,caracterizaremos la movilidad social en sentido restringido.","palabras_clave":"POSCONVERTIBILIDAD|ESTRUCTURA SOCIAL |TRANSFORMACIONES SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2984,"idioma_id":4,"titulo":"Effects of ionizing radiation on Capsicum bacatum var. pendulum (Solanaceae).","resumen":"Cytogenetic and somatic effects of various x-ray treatments were evaluated in pepper, Capsicum baccatum var. pendulum cv. \u201CCayenne\u201D, with the aim to assess optimal conditions for obtaining viable lines. The cytogenetic effects were quantified by counting chromosome aberrations. The level of DNA fragmentation was estimated with TUNEL test (terminal transferase mediated dUTP-fluorescein nick end labeling). Irradiation to 20 Gy with 16-h presoaking can be a suitable treatment of the selected pepper cultivar for a mutagenesis program.","palabras_clave":"CHROMOSOME ABERRATIONS  |DOSE EFFECTS|SOMATIC EFFECTS|TUNEL TEST|X-RAYS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2985,"idioma_id":4,"titulo":"Transcriptional and posttranscriptional inhibition of HMGCR and PC biosynthesis by geraniol in two HEP-G2 cell-proliferation-linked pathways","resumen":"Geraniol, present in the essential oils of many aromatic plants, has in vitro and in vivo antitumor activity againstseveral cell lines. We investigated the effects of geraniol on lipid metabolic pathways involved in Hep-G2 cell proliferation andfound that geraniol inhibits the mevalonate pathway, phosphatidylcholine biosynthesis, cell growth, and cell cycle progression(with an arrest occurring at the G0\/G1 interphase) and increases apoptosis. The expression of 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMGCR), the rate-limiting step in cholesterol synthesis, was inhibited at the transcriptional and posttranscriptionallevels, as assessed by real-time RT\u2013PCR, Western blots, and [14C]HMG-CoA-conversion radioactivity assays. Thatgeraniol decreased cholesterogenesis but increased the incorporation of [14C]acetate into other nonsaponifiable metabolitesindicated the existence of a second control point between squalene and cholesterol involved in redirecting the flow ofcholesterol-derived carbon toward other metabolites of the mevalonate pathway. That exogenous mevalonate failed to restoregrowth in geraniol-inhibited cells suggests that, in addition to the inhibition of HMGCR, other dose-dependent actions existthrough which geraniol can impact the mevalonate pathway and consequently inhibit cell proliferation. These results suggestthat geraniol, a nontoxic compound found in many fruits and herbs, exhibits notable potential as a natural agent for combattingcancer and (or) cardiovascular diseases.","palabras_clave":"HMGCR|GERANIOL|CELL PROLIFERATION|TRANSCRIPTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2986,"idioma_id":4,"titulo":"Limnological changes in two shallow lakes from an urban reserve throughout a complete hydrological cycle: proposals for restauration and management","resumen":"We analysed the fluctuations of the limnological variables and  phytoplankton structure in two shallow lakes located in an urban ecological reserve from Buenos Aires city (Reserva Costanera Sur) during a complete hydrological cycle. The results of this investigation showed that the changes in the water level strongly affect the limnological variables and the structure of the phytoplankton communities. In both shallow lakes, the water level was inversely correlated with conductivity (p < 0.05). During the rising waters, a progressive colonisation by floating macrophytes was observed affecting the dissolved oxygen concentrations. We analysed the fluctuation of the algal groups and dominant species, evaluating the changes in the structure in the  context of the phytoplankton functional groups, and we observed that the water level was one of the main variables explaining the variations in the community. We propose some alternatives for the management and restoration of the shallow lakes of this reserve.","palabras_clave":"URBAN RESERVE|PHYTOPLANKTON|HYDROLOGICAL CYCLE|RESTORATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2987,"idioma_id":4,"titulo":"Identifying clouds over the Pierre Auger Observatory using infrared satellite data","resumen":"We describe a new method of identifying night-time clouds over the Pierre Auger Observatory using infrared data from the Imager instruments on the GOES-12 and GOES-13 satellites. We compare cloud identifications resulting from our method to those obtained by the Central Laser Facility of the Auger Observatory. Using our new method we can now develop cloud probability maps for the 3000 km2 of the Pierre Auger Observatory twice per hour with a spatial resolution of ~2.4 km by ~5.5 km. Our method could also be applied to monitor cloud cover for other ground-based observatories and for space-based observatories.","palabras_clave":"ULTRA-HIGH ENERGY COSMIC RAYS|PIERRE AUGER OBSERVATORY|ATMOSPHERIC MONITORING|CLOUDS: SATELLITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2989,"idioma_id":4,"titulo":"Assessment of Cross-Reactive Host-Pathogen Antibodies in Patients With Different Severity of Chronic Chagas Heart Disease","resumen":"Introduction and objectives  Trypanosoma cruzi infection has been shown to induce humoral autoimmune responses against host antigens tissues. Particularly, antibodies cross-reacting with myocardial antigens may play a role in the development of the severe forms of chronic Chagas disease. The aim of this study was to determine the association between clinical stage of the disease and the presence of autoantibodies in patients with chronic Chagasic disease.    Methods  We performed a cross-sectional study in T. cruzi-seropositive patients divided into 3 groups according to the classic classification of chronic Chagas heart of Storino et al. All participants underwent complete clinical examination and their sera were used to measure autoantibody levels.    Results  All patients had detectable levels of anti-p2&#946; and anti-B13 autoantibodies but none had anti-Na-K-ATPase antibodies. No association was observed between electrocardiographic conduction disturbances and autoantibody levels. Patients with chronic Chagas disease stage III had the highest levels of anti-B13 antibodies and a high risk of mortality score, showing a clear association between disease stage and this score.    Conclusions  Anti-B13 antibodies were significantly higher in chronic Chagas disease stage III patients, suggesting that these antibodies may be involved in disease progression and that they might be a useful marker of poor prognosis in terms of heart compromise. Our results also reveal an important correlation between the level of anti-B13 autoantibodies and symptomatic heart failure and\/or dilated cardiomyopathy.","palabras_clave":"Chagas disease. |Trypanosoma cruzi. |Heart failure. |Anti-B13 autoantibodies."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2990,"idioma_id":4,"titulo":"Pharmacokinetic and pharmacodynamic propereties of enrofloxacin in southern crested caracaras (Caracara plancus)","resumen":"To determine the dosage of enrofloxacin in southern crested caracaras (Caracaraplancus), plasma concentrations of enrofloxacin were measured by high-performance liquidchromatography after intravenous (IV) (5 mg\/kg) and intramuscular (IM) (10 mg\/kg)administration. This compound presented a relatively high volume of distribution (2.09 L\/kg), atotal body clearance of 0.24 L\/kgh, and a long permanence as shown by an elimination half-life of7.81 hours after IV administration and a terminal half-life of 6.58 hours after IM administration.The areas under the concentration-time curves (AUC) were 21.92 and 34.38 lgh\/mL for IM andIV administration, respectively. Enrofloxacin was rapidly absorbed after IM administration with atime to reach maximum concentration of 0.72 hours and bioavailability of 78.76%. After IMadministration, the peak drug concentration (Cmax) was 3.92 lg\/mL. Values of minimuminhibitory concentration (MIC), Cmax, and AUC have been used to predict the clinical efficacy of adrug in treating bacterial infections, with a Cmax\/MIC value of 10 and an AUC\/MIC ratio of 125?250 associated with optimal bactericidal effects. By using the study data and a MIC breakpoint of0.25 lg\/mL, values of Cmax\/MIC were 13.74 and 15.94 and for AUC\/MIC were 90.73 and 139.63,for the IV and IM routes respectively. For the treatment of infectious diseases caused bymicroorganisms with MIC 0.25 lg\/mL, the calculated optimal dosages were 7.5 and 9.5 mg\/kgq24h by the IV and IM routes, respectively. For less susceptible bacteria, a dose increase should beevaluated. To treat caracara by the IV route against microorganisms with MIC 0.25 lg\/mL, thedose should be higher than the 5 mg\/kg used in our study, but possible side effects derived from anincrease in the IV dose and efficacy in sick birds should be assessed","palabras_clave":"PK|enrofloxacin|Caracara plancus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2991,"idioma_id":5,"titulo":"EVALUACIÓN DE Listeria monocytogenes Y CALIDAD MICROBIOLOGICA EN QUESOS FRESCOS DE PRODUCCIÓN ARTESANAL","resumen":"Listeria monocytogenes es un patógeno emergente importante en las enfermedades transmitidas por alimentos, principalmente en productos lácteos como leche y quesos de pasta blanda. Listeria monocytogenes puede crecer en intervalo de pH 4,1 hasta 9,6, siendo su pH óptimo de 7,2 y 7,6; es resistente a concentraciones elevadas de NaCl (30%); no son exigentes nutricionalmente y a temperaturas entre 20ºC y 25ºC son móviles. Debido a su gravedad y letalidad exige un estricto control higiénico de los productos susceptibles de ser contaminados. Los pequeños productores que comercializan en distintas ferias de la ciudad de Posadas y del interior de la provincia de Misiones, elaboran los quesos criollos en forma artesanal, sin estandarización del proceso incluyendo tiempo de coagulación, contenido de sal, humedad final y control de calidad microbiológica. En este sentido, el objetivo de este trabajo fue determinar la incidencia de Listeria spp. y la calidad microbiológica en quesos criollos frescos recolectados en puestos de venta artesanal. El muestreo fue realizado al azar incluyendo quesos de diferentes formas y tamaños a distintas temperaturas de conservación (temperatura ambiente y refrigeración). Se aplicó el método tradicional para la detección cualitativa según la Norma vigente del Código Alimentario Argentino (CAA) y de la Food and Drug Administration Bacteriological Analytical Manual (FDA- BAM). Se analizaron 60 muestras detectándose colonias sospechosas en 18 de ellas. A partir de estas muestras se aislaron 160 cepas utilizando agar Oxford Modificado y Chromagar Listeria como medios selectivos. Luego de las pruebas bioquímicas (Movilidad, Catalasa, Tinción de Gram, Fermentación de azucares, etc), el 17% de las cepas aisladas resultaron positivas para Listeria innocua, y el 6% para Listeria monocytogenes. Los quesos presentaron una calidad higiénica sanitaria regular, con recuentos elevados de Bacterias Aerobias Mesófilas (1,2x104 - 3x108 UFC\/g), Mohos y Levaduras (102 a 7x108 UFC\/g) y presencia de Staphylococcus aureus coag (+) (65%). También se observó contaminación fecal superando el límite permitido por el CAA de Coliformes Totales (30%) y Coliformes Fecales (12%), con presencia de Escherichia coli en el 10% de las muestras. No se encontró presencia de Salmonella spp.. Los resultados reflejan que estos productos presentan deficiencias en la manipulación, envasado y conservación. La presencia de Listeria monocytogenes en alimentos de consumo masivo como los quesos frescos artesanales en esta provincia, representa un riesgo potencial para la salud de los consumidores.","palabras_clave":"CALIDAD MICROBIOLOGICA|QUESO ARTESANAL DE MISIONES|LISTERIA MONOCYTOGENES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2992,"idioma_id":4,"titulo":"First evidence of scavenging of a Glyptodont (Mammalia, Glyptodontidae) from the Pliocene of the Pampean region (Argentina): taphonomic and paleoecological remarks","resumen":"The Cingulata Glyptodontidae (Xenarthra) are one of the most conspicuous Cenozoic herbivore clades in South America reaching North America during the Great American Biotic Interchange. The evidence of predation on these large armoured mammals is very scarce and limited to a Pliocene skull (Glyptotherium) in North America and some latest Pleistocene-early Holocene specimens in South America, with signals of human consumption. In this contribution, we present the first case of scavenging on a glyptodont belonging to cf. Eosclerocalyptus lineatus, (Hoplophorini) from the Pliocene of the Pampean region (Argentina). In addition, we analyze the potential scavengers and the paleoenvironmental context in which this occurred. The evidence suggests that: a) the carcass was covered by a shallow water body, probably an abandoned channel; b) the carcass was completely covered during a brief lapse of time, probably less than a year; c) the morphology of the bite marks clearly coincide with the dentition of the procyonid Chapalmalania (Mammalia, Procyonidae), thus corroborating some presumptions about the paleoautoecological trends of this taxon.","palabras_clave":"South America|Glyptodontidae|Procyonidae|Chapalmalania"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2993,"idioma_id":4,"titulo":"Lichens as bioindicators of air quality in unrban-rural environment in Zavalla","resumen":"An organism is considered a bio-indicator if it can  express some change in response to environmental alterations. Lichens have been  proposed as possible bio-indicators because of their sensitivity to pollutants,  longevity and wide distribution. Zavalla is located at Lat. 33º 1?S and Long.  60º 50?W. This little urban town is surrounded by i) an old park of 100ha named  José F. Villarino with 160 forest species, where the Faculty of Agricultural  Sciences is located since 47 years ago, and ii) plots of agricultural activity.  The aim of this study was to assess the distribution and the seasonal variation  of the lichen populations associated to prevalent tree species of these places  (park and field plots) to find relationships among the lichen colonization,  urban-rural ecosystems, and others factors that allow detecting risks of air pollution.  Five and three sites placed in the park and field plots, respectively; were  evaluated. Observations were done in winter and spring-summer 2012, and  summer-autumn 2013. Evaluation of each tree side (oriented to north, east,  south and west) was performed with a grid (14x14 cm2) placed at 160cm height  ground. Percentage of Colonized Area was the variable used. Analysis of  Variance and Fisher LSD Test were used to detect significant differences among  seasons, tree faces and species. There were differences between tree sides,  being the southern the most colonized by lichens. Moreover there were  differences between tree species, being Brachychiton populneum the most  colonized. Results showed also that there were no differences as between park and  field plots as among seasons.","palabras_clave":"LICHENS|BIOINDICATORS|AIR QUALITY|ZAVALLA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2994,"idioma_id":5,"titulo":"Isotermas de Adsorción y Desorción de Agua en Leche Descremada en Polvo","resumen":"El objetivo de este trabajo fue determinar las isotermas de adsorción y desorción de leche descremada en polvo  a diferentes temperaturas. Se realizaron las experiencias a 15ºC, 25ºC y 40ºC, en un amplio rango de  humedades y actividades de agua propias de las condiciones habituales en que se desarrollan los procesos de  secado, envasado y almacenamiento de la leche descremada en polvo. Los datos experimentales fueron  correlacionados con dos modelos de ecuaciones referenciales: Modelo de GAB y Modelo de BET. Tanto para la  adsorción como para la desorción se observó un mejor ajuste con el modelo de GAB. En particular, para la  desorción, se pudo apreciar una buena correlación en las actividades de agua menores que 0,5 con el modelo  de BET. Se observó un entrecruzamiento de las isotermas de adsorción a distintas temperaturas, lo cual influye  en la relación entre el calor isostérico de adsorción en función del contenido de humedad.","palabras_clave":"ACTIVIDAD DE AGUA|ISOTERMAS DE SORCION|CONTENIDO DE HUMEDAD|CALOR ISOSTERICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2995,"idioma_id":5,"titulo":"Registro de un dinosaurio Aeolosaurini (sauropoda, Titanosauria) en el Cretácico Superior (Formación Plottier) del norte de la Provincia del Neuquén, Argentina, y comentarios sobre los aeolosaurini sudamericanos.","resumen":"The clade Aeolosaurini is represented by several specimens found, so far,only in Argentina and Brazil. The material reported here corresponds to a sauropod titanosaur consisting of four incomplete anterior caudal vertebrae, from the Narambuena Paleontological Site, Rincón de los Sauces, Neuquén Province, Argentina. The specimen comes from the Plottier Formation (late Coniacian-lower Santonian, Upper Cretaceous), Neuquén Group. The specimen has a combination of features that includes it into the clade Aeolosaurini: anterior caudal centra with anterodorsal margin bent forward; prezygapophyses elongated in anterior caudal, in correlation with the extreme displacement of the neural arch forward; and anteroposteriorly elongated articular facets of prezygapophyses, at least in the anterior caudals. However, it differs from other Aeolosaurini by having postzygapophysis with anteroposteriorly short articular facets, not as elongated in the prezygapophyses. This specimen corresponds not only to the first discovery of an Aeolosaurini in the north of the Neuquén Basin (because Rinconsaurus caudamirus Calvo & Gonzalez Riga has been included in another clade, Rinconsauria), but also the oldest record of the group.","palabras_clave":"Dinosauria|Aeolosaurini|late Coniacian-lower Santonian|Plottier Formation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2997,"idioma_id":4,"titulo":"Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in $\\sqrt{s}=7$ TeV $pp$ collisions with the ATLAS detector","resumen":"A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb-1 of sqrt(s) = 7 TeV proton-proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results.","palabras_clave":"ATLAS|CERN|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":2998,"idioma_id":4,"titulo":"OCURRENCE AND DISTRIBUTION OF SOIL FUSARIUM SPP. POPULATION UNDER WHEAT CROP IN ZERO TILLAGE","resumen":"The presence of Fusarium species in cultivated soils is commonly associated with plant debris and plant roots. Fusarium species are also soil saprophytes. The aim of this study was to examine the occurrence and distribution of soil Fusarium spp. at different soil depths in a zero tillage system after the wheat was harvested. Soil samples were obtained at three depths (0-5 cm; 5-10 cm and 10-20 cm) from five crop rotations: I: Conservationist Agriculture: Wheat-Sorghum-Sunflower; II: Mixed agriculture\/livestock with pastures (Without usingwinter or summer forages): Wheat-Sorghum-Soybean-Canola-Pastures, III: Winter agriculture in depth limited soils: Wheat-Canola-Barley-late soybean, IV: Mixed with annual forage: Wheat-Oat\/Vicia-Sunflower; V: Intensive agriculture: Wheat-Barley-Canola, with alternation of soybean or late soybean. One hundred twenty two isolates of Fusarium were obtained and assigned as F. equiseti, F. merismoides, F. oxysporum, F. scirpi and F. solani. The most prevalent species was F. oxysporum, wich was observed in all sequences and depths. The Tukey¡¦s test showed that the relative frequency of F. oxysporum under intensive agricultural management was higher than in mixed traditional ones. The first 5 cm of soil showed statistically significant differences respect to 5-10 cm and 10-20 cm depths. The ANOVA test for the relative frequency of F. equiseti, F. merismoides, F. scirpi and F. solani, did not show statistically significant differences. We not found significant differences in the effect of crop rotations and depth on Shannon, Simpson indexes and species richness. Therefore we conclude that the different sequences and the sampling depth did not affect the alpha diversity of Fusarium community in this system.","palabras_clave":"FUSARIUM|SOIL|ZERO TILLAGE|CROP ROTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3057,"idioma_id":4,"titulo":"A prospective study to assess the performance of the improved Boron Neutron Capture Therapy Facility in Argentina","resumen":"From 2008 to 2011, several planned modifications were implemented at the RA-6 reactor in Argentina, leading to significant benefits for future BNCT treatments. New capabilities have been implemented in NCTPlan treatment planning system. To assess the performance of the new BNCT facility, a dosimetric reevaluation of previous clinical cases was performed, taking into account the modifications carried out in the new facility and compared the results of the original treatment plans with optimized plans that are considered as feasible patient setups.","palabras_clave":"BNCT|RA-6 reactor|Treatment planning|Melanoma trials"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3065,"idioma_id":5,"titulo":"'Otro territorio: emergencia, controversias y perspectiva en torno al emergente sector de medios públicos en Argentina a partir del año 2009'","resumen":"En el marco del proyecto de investigación titulado ?Políticas de Comunicación y Cultura en la Argentina contemporánea. Reflexiones sobre subalternidad y poder en torno al emergente sistema de medios públicos audiovisuales? -radicado en la Universidad Nacional de Villa María- nuestro propósito es introducir una serie de nociones que permitan caracterizar el sector de medios públicos audiovisuales emergente en Argentina: lo que implica una revisión de la Ley 26.522\/09, su estructura organizativa y las condiciones de desarrollo del sector. Asimismo tal descripción se articula con una reflexión más amplia sobre la función, misión y objetivos de los medios públicos en una sociedad democrática, esto es la arquitectura del espacio público mediático en términos de acceso, participación y pluralismo.    A partir de una convergencia de enfoques teóricos que provienen de la Economía Política de la Comunicación, los Estudios Culturales y los Estudios Poscoloniales buscamos producir interpretaciones acerca de la aplicación de Políticas de Comunicación y Cultura en Argentina -y otros países de la región- en torno al sistema de medios públicos audiovisuales, y de cómo tales políticas restringen o habilitan la emergencia de actores y sus disputas por el sentido considerando situaciones de subalternidad y vínculos con el poder.","palabras_clave":"AUDIOVISUAL|POLÍTICAS|MEDIOS PÚBLICOS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3066,"idioma_id":4,"titulo":"Morphological and Anatomical Development of Fruits of Two Varieties of Olive (Olea europaea L.) Growing Under Irrigated and Rainfed Conditions in the Southwest of Buenos Aires Province.","resumen":"This study compared the fruit development of the olive varieties Arbequina and Nevadillo grown under irrigated and rainfed conditions in the Southwest of Buenos Aires province (38º50?21? S; 61º06?46? W). From fruit set to harvest, seven fruit sampling dates were selected, measuring fruit volume and mesocarp and endocarp transverse area (TA). Digital images of histological sections of the mesocarp were analyzed by counting cell number and cell TA. During the experimental period, rain accounted for 511 mm (rainfed). Water supplied by irrigation totalized 222 mm. The fruit growth dynamics based on the volume and the TAfruit in both varieties fit to a double sigmoid function with two phases of rapid growth connected by a plateau between January and March. Throughout the study, plant water status, monitored by the RWCleaf, was similar among water regimes. The TAendocarp was similar between varieties and water regimes. At final fruit volume, TAfruit and TAmesocarp showed significant differences between varieties, being of 2.48 cm3, 165 mm2 and 126 mm2 in Nevadillo and 1.36 cm3, 129 mm2 and 93 mm2 in Arbequina, respectively. Final TAcell was similar in both varieties averaging 6050 &#61549;m2. The mesocarp cell number was higher in Nevadillo, which may explain its higher TAmesocarp. Fruits grown under rainfed had higher TAfruit, TAmesocarp, cell number and TAcell than those grown under irrigation. Lower crop load in rainfed trees compared to irrigated ones could explain this last result.","palabras_clave":"OLEA EUROPAEA L.|OLIVE|FRUIT GROWTH|IRRIGATION|MESOCARP|CELL NUMBER|CELL SIZE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3067,"idioma_id":5,"titulo":"Control cognitivo de la emoción: funciones ejecutivas implicadas en el uso de la reevaluación cognitiva como estrategia de regulación emocional.","resumen":"El objetivo de este trabajo es presentar un tópico de discusión actual en el área de la regulación emocional que se refiere a entender qué papel tendrían las variables de funcionamiento ejecutivo en el control cognitivo de la emoción. Para esto, se describe en primer lugar, el Modelo de Proceso de Regulación Emocional (Gross & Thompson, 2007). En segundo  lugar, se presentan las relaciones que se han planteado teóricamente entre una de las estrategias de regulación emocional que ha mostrado fuertes asociaciones con el bienestar subjetivo de los individuos ? específicamente la revaluación cognitiva- y las variables de control cognitivo que serían requeridas para su ejecución eficiente ?específicamente memoria de trabajo, inhibición y flexibilidad cognitiva. Se concluye con la necesidad de contar con más estudios que exploren  estas asociaciones debido a la poca exploración que ha recibido hasta el momento y a su importancia para la ampliación del entendimiento de la regulación emocional.","palabras_clave":"regulación emocional|reevaluación cognitiva|control cognitivo|memoria de trabajo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3071,"idioma_id":4,"titulo":"Characterization of a Variant of Xanthomonas citri subsp. citri   that Triggers a Host-Specific Defense Response","resumen":"Citrus is an economically important fruit crop that is severely afflicted   by Asiatic citrus bacterial canker (CBC), a disease caused by the phyto-  pathogen Xanthomonas citri subsp. citri (X. citri). To gain insight into the   molecular epidemiology of CBC, 42 Xanthomonas isolates were collected   from a range of  Citrus spp. across 17 different orchards in Tucumán,   Argentina  and subjected to molecular, biochemical, and pathogenicity   tests. Analysis of genome-specific  X. citri markers and DNA polymor-  phisms based on repetitive elements-based polymerase chain reaction   showed that all 42 isolates belonged to X. citri. Interestingly, pathogeni-  city tests showed that one isolate, which shares >90% genetic similarity   to the reference strain X. citri T, has host range specificity. This new variant   of X. citri subsp.  citri, named X. citri AT, which is deficient in xanthan   production, induces an atypical, noncankerous chlorotic phenotype in   Citrus limon and C. paradisi and weak cankerous lesions in C. auranti-  folia and C. clementina leaves. In C. limon, suppression of canker devel-  opment is concomitant with an oxidative burst; xanthan is not implicated   in the phenotype induced by this interaction, suggesting that other bac-  terial factors would be involved in triggering the defense response.","palabras_clave":"GENETIC DIVERSITY|REP-PCR|FINGERPRINTING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3072,"idioma_id":4,"titulo":"Hormonal Modulation of Hepatic cAMP Prevents Estradiol 17&#946;-d-Glucuronide-Induced Cholestasis in Perfused Rat Liver","resumen":"            BACKGROUND:  Estradiol-17&#946;-D-glucuronide (E17G) induces cholestasis in vivo, endocytic  internalization of the canalicular transporters multidrug resistance-associated  protein 2 (Abcc2) and bile salt export pump (Abcb11) being a key  pathomechanism. Cyclic AMP (cAMP) prevents cholestasis by targeting these  transporters back to the canalicular membrane. In hepatocyte couplets, glucagon  and salbutamol, both of which increase cAMP, prevented E17G action by  stimulating the trafficking of these transporters by different mechanisms,  namely: glucagon activates a protein kinase A-dependent pathway, whereas  salbutamol activates an exchange-protein activated by cAMP (Epac)-mediated,  microtubule-dependent pathway.            METHODS: The  present study evaluated whether glucagon and salbutamol prevent E17G-induced  cholestasis in a more physiological model, i.e., the perfused rat liver (PRL).  Additionally, the preventive effect of in vivo alanine administration, which  induces pancreatic glucagon secretion, was evaluated.            RESULTS: In  PRLs, glucagon and salbutamol prevented E17G-induced decrease in both bile flow  and the secretory activity of Abcc2 and Abcb11. Salbutamol prevention fully  depended on microtubule integrity. On the other hand, glucagon prevention was  microtubule-independent only at early time periods after E17G administration,  but it was ultimately affected by the microtubule disrupter colchicine.  Cholestasis was associated with endocytic internalization of Abcb11 and Abcc2,  the intracellular carriers being partially colocalized with the endosomal  marker Rab11a. This effect was completely prevented by salbutamol, whereas some  transporter-containing vesicles remained colocalized with Rab11a after glucagon  treatment. In vivo, alanine administration increased hepatic cAMP and  accelerated the recovery of bile flow and Abcb11\/Abcc2 transport function after  E17G administration. The initial recovery afforded by alanine was microtubule-independent,  but microtubule integrity was required to sustain this protective effect.            CONCLUSION: We  conclude that modulation of cAMP levels either by direct administration of cAMP  modulators or by physiological manipulations leadings to hormone-mediated increase  of cAMP levels (alanine administration), prevents estrogen-induced cholestasis  in models with preserved liver architecture, through mechanisms similar to  those arisen from in vitro studies.  ","palabras_clave":"Mrp2|Bsep|Salbutamol|Glucagon"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3074,"idioma_id":4,"titulo":"Preservation of Solanum pimpinellifolium genomic fragments in recombinant genotypes improved the fruit quality of tomato","resumen":"Preservation of Solanum pimpinellifolium genomic fragments in recombinant genotypes improved the fruit quality of tomato","palabras_clave":"PLANT GENETIC RESOURCES|SECOND CYCLES HYBRIDS|SRAP|GENERALIZED PROCRUSTES ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3075,"idioma_id":4,"titulo":"Grazing-induced  morphological  and  growth  rate  changes  in  Anarthrophyllum rigidum,  a  Patagonian  leguminous  shrub","resumen":"Grazing in&#64258;uences the morphology and growth rate of shrubs, and consequently, their population dynam-ics. It has been shown that grazing directly affects the growth of shrubs. On the other hand, the reduction of grass biomass by herbivores reduces soil?water competition between grasses and shrubs, and indirectly, could enhance the growth of shrubs. However, the assessment of the long-term effects of grazing on the growth of shrubs in the arid Patagonia has been hampered by the lack of long and homogeneous records of plant population dynamics and primary production. In this study, we combined growth-ring and allo-metric analyses to assess the long-term effect of grazing on individuals of Anarthrophyllum rigidum, a leguminous shrub widely distributed across the Patagonian steppe. A. rigidum has evergreen leaves rich in proteins that constitute an important complement to the diet of sheep, particularly in winter when the abundance of grasses is reduced. Our observations indicate that individuals of A. rigidum nearby the water source used by livestock were smaller in size (35.5 cm vs. 67.39 cm), presented a larger number of basal branches (23 vs. 12), and showed slower rates of growth (8.2 mm year&#8722;1 vs. 14.3 mm year&#8722;1) than individuals located far from the water source. This &#64257;rst quanti&#64257;cation of the long-term effects of grazing on A. rigidum in the dry Patagonian steppe suggests that bene&#64257;cial effects of grazing through the reduction of grasses that compete with shrubs for soil?water should be more obvious for livestock non-preferred than preferred shrubs.","palabras_clave":"Growth-ring analysis |Allometric traits |Herbivore preference |Piosphere"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3076,"idioma_id":4,"titulo":"Investment Decisions in Distribution Networks under Uncertainty with Distributed Generation - Part II: Implementation and Results","resumen":"Investment in distributed generation (DG) is an attractive distribution planning option for adding flexibility to an expansion plan, mainly by deferring network reinforcements. In this first part of a two-part paper, a risk-based optimization approach is proposed to model a multistage distribution expansion planning problem that takes DG into account as a flexible option to temporarily defer large network investments. Five features of the installation of DG related to location, size, type, operation and timing are all optimized. The evolutionary particle swarm optimization (EPSO) method is applied to solve this mixed integer nonlinear problem. A return-per-risk index is proposed to assess expansion investments. This index achieves an efficient synergy between the expected return and the risk of investments by performing Monte Carlo simulations. In turn, the flexibility of network investment deferral is assessed through a real option valuation. Finally, in order to quantify the investment deferral benefit, the expected return from a traditional expansion plan (without DG) is compared to the return obtained from a flexible expansion plan (with DG). In the companion paper, the proposed approach is tested on a typical Latin American distribution network; implementation aspects and analysis of numerical results are presented.","palabras_clave":"Distributed generation|EPSO|distribution planning|expansion decisions|real options|risk analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3077,"idioma_id":4,"titulo":"Induction and Persistence of Large &#947;H2AX Foci by High Linear Energy Transfer Radiation in DNA-dependent protein kinase-Deficient Cells","resumen":"Purpose  To evaluate the cell response to DNA double-strand breaks induced by low and high linear energy transfer (LET) radiations when the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), an essential protein of the nonhomologous end-joining repair pathway, lacks kinase activity.    Methods and Materials  CHO10B2, a Chinese hamster ovary cell line, and its derived radiosensitive mutant cell line, irs-20, lacking DNA-PKcs activity, were evaluated after 0 to 3 Gy of &#947;-rays, plateau and Bragg peak protons, and lithium beams by clonogenic assay, and as a measurement of double-strand breaks, phosphorylated H2AX (&#947;H2AX) foci number and size were quantified by immunocytofluorescence.    Results  Irs-20 exhibited greater radiosensitivity and a higher amount of &#947;H2AX foci than CHO10B2 at 6 hours after irradiation for all types of radiations. Remarkably, CHO10B2 and irs-20 maintained their difference in radiosensitivity after high-LET radiation. Six hours after low-LET radiations, irs-20 did not reach basal levels of &#947;H2AX at high doses, whereas CHO10B2 recovered basal levels for all doses. After high-LET radiation, only CHO10B2 exhibited a reduction in &#947;H2AX foci, but it never reached basal levels. Persistent foci in irs-20 confirmed a repair deficiency. Interestingly, after 30 minutes of high-LET radiation both cell lines exhibited large foci (size >0.9 &#956;m2) related to the damage nature, whereas at 6 hours irs-20 showed a higher amount of large foci than CHO10B2, with a 7-fold increase at 3 Gy, that could also be associated to radiosensitivity.    Conclusions  We demonstrated, for the first time, an association between deficient DNA-PKcs activity and not only high levels of H2AX phosphorylation but also persistence and size increase of &#947;H2AX foci after high-LET irradiation.","palabras_clave":"gamma H2AX Foci|High LET particles|protons and Li ions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3079,"idioma_id":4,"titulo":"Molecular mechanisms involved in interleukin 1-beta (IL-1&#946;)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (&#945;-MSH)","resumen":"Pro-inflammatory cytokines can affect cognitive processes such as learning and memory. Particularly,interleukin-1b (IL-1b) influences the consolidation of hippocampus-dependent memories. We previously reported that administration of IL-1b in dorsal hippocampus impaired contextual fear memory consolidation. Different mechanisms have been implicated in the action of IL-1b on long-term potentiation (LTP), but the processes by which this inhibition occurs in vivo remain to be elucidated. We herein report that intrahippocampal injection of IL-1b induced a significant increase in p38 phosphorylation after contextual fear conditioning. Also, treatment with SB203580, an inhibitor of p38, reversed impairment induced by IL-1b on conditioned fear behavior, indicating that this MAPK would be involved in the effect of the cytokine.We also showed that IL-1b administration produced a decrease in glutamate release from dorsal hippocampus synaptosomes and that treatment with SB203580 partially reversed this effect. Our results indicated that IL-1b-induced impairment in memory consolidation could be mediated by a decrease in glutamate release. This hypothesis is sustained by the fact that treatment with D-cycloserine (DCS), a partial agonist of the NMDA receptor, reversed the effect of IL-1b on contextual fear memory. Furthermore, we demonstrated that IL-1b produced a temporal delay in ERK phosphorylation and that  DCS administration reversed this effect. We also observed that intrahippocampal injection of IL-1b decreased BDNF expression after contextual fear conditioning. We previously demonstrated that alpha-  MSH reversed the detrimental effect of IL-1b on memory consolidation. The present results demonstrate that alpha-MSH administration did not modify the decrease in glutamate release induced by IL-1b. However,intrahippocampal injection of alpha-MSH prevented the effect on ERK phosphorylation and BDNF expression induced by IL-1b after contextual fear conditioning. Therefore, in the present study we determine possible  molecular mechanisms involved in the impairment induced by IL-1b on fear memory consolidation. We also established how this effect could be modulated by alpha-MSH.","palabras_clave":"IL-1 beta|MEMORY CONSOLIDATION|ALPHA-MSH|GLUTAMATE RELEASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3080,"idioma_id":4,"titulo":"Improving the photosensitizing properties of ruthenium polypyridyl complexes by using 4-methyl-2,2?-bipyridine-4?-carbonitrile as an auxiliary ligand","resumen":"We   report in this work the synthesis and spectroscopic, electrochemical,   spectroelectrochemical, and photophysical characterization of a novel   series of ruthenium polypyridyl complexes with   4-methyl-2,2&#8242;-bipyridine-4&#8242;-carbonitrile (Mebpy-CN) as an auxiliary   ligand of general formula[Ru(bpy)3&#8722;x(Mebpy-CN)x](PF6)2(x=1&#8722;3) (with bpy =   2,2&#8242;-bipyridine). A significant increase in the lifetime and quantum   yield of emission of the lowest 3MLCT excited state is disclosed when   going from x=1 to x=   3, evidencing an improvement of the photosensitizing properties with   respect to [Ru(bpy) 3]-(PF6)2. Furthermore, quenching by molecular   oxygen of 3MLCT excited statesof the three complexes produced singlet   molecular oxygen (1O2) with quantum yield values higher than that of   [Ru(bpy)3]2+ in CH3CN. The structure of the complex with x= 1 has been   determined by X-ray diffraction. The photoconductivity of ZnO nanowires   covered with this same complex is increased by an order of magnitude,   pointing to its feasibility as a component of a DSSC. A new dinuclear   complex with Mebpy-CN as a bridging ligand has also been prepared and   characterized by physicochemical techniques. The derived mixed-valent   species of formula [(bpy)2 RuII(Mebpy-CN)RuIII(NH3)5]5+dis-plays a   considerable metal&#8722;metal electronic coupling due to the delocalization   effect of a nitrile group in the 4&#8242; position of the bpy ring.","palabras_clave":"Ruthenium Polypyridyl Complexes|Photosensitizing|ZnO nanostructures|Quantum yield"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3081,"idioma_id":4,"titulo":"Emergence and spread of tet(B)::ISCR2 plasmid-borne in minocycline resistance Acinetobacter baumannii isolates","resumen":"Resistance to minocycline has emerged in multidrug-resistant Acinetobacter baumannii isolates from Buenos Aires hospitals. Few reports about the description and dispersion of tet genes in this species have been published. We observed the presence of tet(B) in all minocycline-resistant isolates. This gene was found to be associated with the ISCR2 mobile element, which may, in part, explain its dispersion.","palabras_clave":"Acinetobacter baumannii|ISCR2|resistencia minociclina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3082,"idioma_id":4,"titulo":"Slow epidemic extinction in populations with heterogeneous infection rates","resumen":"We explore how heterogeneity in the intensity of interactions between people affects epidemic spreading. For that, we study the susceptible-infected-susceptible model on a complex network, where a link connecting individuals i and j is endowed with an infection rate &#946;ij=&#955;wij proportional to the intensity of their contact wij, with a distribution P(wij) taken from face-to-face experiments analyzed in Cattuto et al. [ PLoS ONE 5 e11596 (2010)]. We find an extremely slow decay of the fraction of infected individuals, for a wide range of the control parameter &#955;. Using a distribution of width a we identify two large regions in the a-&#955; space with anomalous behaviors, which are reminiscent of rare region effects (Griffiths phases) found in models with quenched disorder. We show that the slow approach to extinction is caused by isolated small groups of highly interacting individuals, which keep epidemics alive for very long times. A mean-field approximation and a percolation approach capture with very good accuracy the absorbing-active transition line for weak (small a) and strong (large a) disorder, respectively.","palabras_clave":"NETWORKS|DISORDER|EPIDEMICS|COMPLEXITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3083,"idioma_id":4,"titulo":"Revision of the systematic status of patagonian and pampean gray foxes (Canidae: Lycalopex griseus and Lycalopex gymnocercus) using 3 d geometric morphometrics","resumen":"Argentinean ?zorros de campo? are currently included in two species: Lycalopex griseus and L. gymnocercus. Lycalopex gymnocercus lives in northern Patagonia and in most of central and northern Argentina. Lycalopex griseus is smaller and lives in Patagonia and throughout western Argentina. A previous traditional morphometric study using cranio-dental measurements considered both forms to be the same species, showingclinal reduction in size from northeastern to southwestern Argentina. Here we tested the synonymy of these foxes and the existence of clinal variation using a large sample and geometric morphometric methods. Our results rejected the separation of these foxes in two different species and confirmed, based on cranium and mandible size and shape that they belong to the same species. Also, we show there is a clinal variation in size that has an allometric component in cranial and mandibular shape, which accounts for the differences between these foxes.","palabras_clave":"Geometric morphometrics|Systematics|Canidae|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3084,"idioma_id":5,"titulo":"Valorización de la naturaleza y disputas por el territorio en la Provincia de Santa Cruz","resumen":"Desde los inicios de la colonización europea, la historia de la Patagonia es la trayectoria de la ocupación para la puesta en valor de la riqueza de sus bienes naturales en el suelo, subsuelo y en su extenso litoral marítimo. Asentados primero en el ovino, luego en el petróleo, el gas y el carbón y recientemente la pesca, la minería metalífera y el turismo internacional; los territorios de las actuales provincias de Santa Cruz, del Chubut y de Tierra del Fuego, al igual que sus pares trasandinos de las regiones de Aysén y Magallanes, son espacios ricos en recursos. Para la puesta en valor de los territorios por parte del capital hacen falta dos condiciones básicas como son la existencia de bienes naturales y de fuerza de trabajo. Estas condiciones, dependiendo de la actividad que se pretenda analizar, pueden o no estar presentes en el territorio. En algunos casos, se hace necesario adecuar estos espacios mediante equipamientos e infraestructuras, la atracción de trabajadores de diversa calificación y la presencia estatal en un doble proceso de expansión geográfica y acumulación por desposesión que asegure el funcionamiento del proceso de valorización en general. En estos espacios, periféricos desde el punto de vista espacial, cada proceso de valorización particular exigió una combinación de apropiación, habilitación y organización del territorio específicas. Actualmente y en términos globales, los habitantes de la región gozan de altos niveles de vida en términos relativos con las áreas centrales de sus respectivos espacios nacionales, pero se trata de sociedades cada vez más duales y espacios cada vez más fragmentados. En este territorio histórica y actualmente conflictivo y disputado en su apropiación y puesta en valor, en el discurso del Estado y las empresas: desarrollo equivale a profundización del modelo primario exportador y rentista. En el presente artículo se apunta, en primer lugar, a presentar sintéticamente los procesos históricos de valorización de la naturaleza para luego analizar la actual situación del proceso en el territorio y los conflictos resultantes. Al tener en cuenta las tendencias mundiales de alta demanda de bienes naturales se apuntará, finalmente, a exponer los escenarios más probables en caso de acentuarse el actual modelo extractivo y rentista.","palabras_clave":"BIENES COMUNES |CONFLICTOS DISTRIBUTIVOS |POLÍTICAS PÚBLICAS |DINÁMICAS MUNDIALES |TERRITORIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3085,"idioma_id":4,"titulo":"Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)","resumen":"Endosulfan (ES), a persistent organochlorine pesticide, is widely used despite its toxicity to non-target  animals. Upon reaching water bodies, ES can cause negative effects on aquatic animals, including disruption  of hormonal systems. However, the action of ES on fish reproductive axis has been hardly studied  thus far. The aim of the present work was to assess the endocrine disruptive potential of endosulfan on the  pituitary gonadotropins levels and on the testes function due to ES in the South American freshwater fish  Cichlasoma dimerus, using in vitro and in vivo approaches. In vitro experiments showed that ES inhibited  the LH-stimulated steroidogenesis in gonads; no change was observed in gonadotropins release from  pituitaries in culture. Laboratory waterborne ES (0.1, 0.3 and 1 g\/L) exposure for two months caused  decrease in FSH pituitary content and GT activity in the testes (Sertoli cell function marker). Testicular  histology revealed pathologies such as scarce intermediate stages of spermatogenesis, release of  immature germ cells into the lobular lumen, presence of foam cells and interstitial fibrosis. As FSH and  FSH-mediated steroidogenesis regulate spermatogenesis and Sertoli cell function, the effect of ES on FSH  could be responsible for the morphological alterations observed in testes. In vitro, ES disrupted steroidogenesis  in gonads, therefore similar effects in vivo cannot be ruled out. Based on this evidence, ES exhibits  an endocrine disruptive action on the reproductive axis of C. dimerus, causing disruption at the pituitary  and\/or at the gonad level. These effects could acquire ecological significance under prolonged exposure  to the pesticide in nature.","palabras_clave":"endosulfan|gonadotrofinas|hormonas esteroideas|pez ciclido"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3086,"idioma_id":4,"titulo":"Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic","resumen":"        Extracellular hydrolytic enzyme-producing  bacteria are known to play a key role in degradation of sedimentary particulate  organic matter and both, their microbial diversity and enzyme production have  begun to be fully studied. In this work, diversity of culturable  protease-producing bacteria isolated from different marine sources from Potter  Cove (South Shetland Islands, Antarctica) and their ability to produce  economically important enzymes (amylases, pectinases, xylanases, cellulases and  carboxymethyl-cellulases) were investigated. In total, 88 proteolytic bacterial  strains were obtained and grouped in 25 phylotypes based on their  characterization by Amplified Ribosomal DNA Restriction Analysis (ARDRA).  Analysis of the 16S rRNA gene sequences of representatives of the 25 phylotypes  showed that the predominant cultivated protease-producing bacteria are Gammaproteobacteria affiliated to the  genera Pseudomonas, Shewanella, Colwellia, and Pseudoalteromonas,  which was the predominant group (64%). In addition, representatives of the  phyla Actinobacteria, Bacilli and Flavobacteria were found. Among the 88 isolates screened, more than  85% showed at least one extracellular hydrolytic enzyme activity. We detected  21, 67, 53, 68, and 55 isolates positive for amylases, pectinases, cellulases,  CM-cellulases and xylanases, respectively, with some of them producing up to  five extracellular enzymes.  ","palabras_clave":"ANTARCTIC|MARINE BACTERIA|COLD ENZYMES|PSYCHROPHILES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3087,"idioma_id":4,"titulo":"Comparison of Delayed Hydride Cracking DHC Behaviour of Two Zirconium Alloys","resumen":"Delayed hydride cracking (DHC) is an important failure mechanism that may occur in Zr alloys duringservice in water-cooled reactors. Two conditions must be attained to initiate DHC from a crack: the stressintensity factor must be higher than a threshold value called KIH and, hydrogen concentration mustexceed a critical value. Currently the pressure tubes for CANDU reactor are fabricated from Zr?2.5Nb.In this paper the critical hydrogen concentration for DHC and the crack velocity of a developmental pressuretube, Excel, was evaluated and compared with that of Zr?2.5Nb. The DHC velocity values measuredin Excel were higher than usually reported in Zr?2.5Nb. Due to the higher hydrogen solubility limits inExcel, its critical hydrogen concentration for DHC initiation is 10?50 wppm over that of Zr?2.5Nb inthe range of 150?300 C.","palabras_clave":"DELAYED HYDRIDE CRACKING|ZR ALLOYS|CRITICAL CONCENTRATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3089,"idioma_id":4,"titulo":"Catalysts based on Rh(III)-hexamolybdate \/&#61543;-Al2O3 and their application in the selective hydrogenation of cinnamaldehyde to Hydrocinnamaldehyde","resumen":"Several catalysts based on [RhMo6O24H6]3&#8722; (RhMo6) heteropolyanion supported on &#947;-Al2O3 with different textural properties were prepared, characterized and tested in the liquid-phase hydrogenation of cinnamaldehyde. The characterization of pure and supported systems was carried out using several techniques including XRD, SEM-EDS microscopy, Raman microprobe, X-ray photoelectron spectroscopies and temperature programmed reduction (TPR). The catalytic performance was monitored by conversion of the starting cinnamaldehyde as a function of time: the initial activities represented as the turnover frequency (TOF), were measured considering the surface Rh atoms. The planar heteropolyanion RhMo6\/(EI) based systems showed enhanced catalytic activity over the RhMo conventional catalyst obtained by successive impregnation of both transition metal ions (Rh(III) chloride and heptamolybdate). The selectivity for the RhMo6 systems was mainly toward HCAL unlike the conventional catalyst which showed selectivity toward CA. This study also showed a synergetic effect between Rh and Mo through which Rh promoted Mo reducibility.","palabras_clave":"HETEROPOLYOXOMOLYBDATES|ANDERSON PHASES OF Rh(III)|RhMO6\/&#947;-Al2O3|CINNAMALDEHYDE|SELECTIVE HYDROGENATION|HYDROCINNAMALDEHYDE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3090,"idioma_id":4,"titulo":"Levels of inflammatory cytokines, adrenal steroids, and mRNA for GR&#945;, GR&#946; and 11&#946;HSD1 in TB pleurisy","resumen":"Our previous work on the immune-endocrine features of patients with pulmonary tuberculosis (TB)showed markedly decreased plasma levels of dehydroepiandrosterone (DHEA) together with augmented concentrations of Cortisol and pro- and anti-in&#64258;ammatory cytokines. Studies in peripheral bloodmononuclear cells (PBMC) indicated a lower mRNA a\/b ratio of glucocorticoid receptors -GR- together with a higher 11b-hydroxysteroid dehydrogenase type 1 (11bHSD1) mRNA expression in cases with severe pulmonary TB. Since Pleural TB (PLTB) is a rather benign manifestation of TB, we nowanalyzed the systemic and local immune-endocrine pro&#64257;le as well as the GRa,GRb,11bHSD1 and 11bHSD2 transcripts in PBMC and pleural effusion mononuclear cells (PEMC) of patients with PLTB. PLTB patients had increased levels of IL-1b, IL-6 and IFNg together with reduced Cortisol and DHEA concentrations in pleural &#64258;uids. Also, a signi&#64257;cantly increased expression of 11bHSD1 and GRa was found in PEMCcompared to PBMC. Findings point out to an appropriate immune response and a substantial in&#64258;ammatory reaction, wherein the low Cortisol concentrations may be equally effective, because of thebvincreased expression of GRa and 11bHSD1 transcripts which may optimize the immunomodulatory properties of Cortisol.","palabras_clave":"Pleural tuberculosis|Immunoendocrine interaction|Cytokines|Glucocorticoid receptor|11bHSD1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3091,"idioma_id":4,"titulo":"In MMTV-Her-2\/neu transgenic mammary tumors the absence of caveolin-1 (-\/-) alters PTEN and NHERF1 but not beta-catenin expression","resumen":"In a recent study, we have shown that in mammary tumors from mice lacking the Cav-1 gene, there are alterations in specific heat shock proteins as well as in tumor development. With this in mind, we have now investigated other proteins in the same mammary mouse tumor model (Her-2\/neu expressing mammary tumors from Cav-1 wild type and Cav-1 null mice), to further comprehend the complex tumor-stroma mechanisms involved in regulating stress responses during tumor development. In this tumor model the cancer cells always lacked of Cav-1, so the KO influenced the Cav-1 in the stroma. By immunohistochemistry, we have found a striking co-expression of &#946;-catenin and Her-2\/neu in the tumor cells. The absence of Cav-1 in the tumor stroma had no effect on expression or localization of &#946;-catenin and Her-2\/neu. Both proteins appeared co-localized at the cell surface during tumor development and progression. Since Her-2\/neu activation induces MTA1, we next evaluated MTA1 in the mouse tumors. Although this protein was found in numerous nuclei, the absence of Cav-1 did not alter its expression level. In contrast, significantly more PTEN protein was noted in the tumors lacking Cav-1 in the stroma, with the protein localized mainly in the nuclei. P-Akt levels were relatively low in tumors from both Cav-1 WT and Cav-1 KO mice. There was also an increase in nuclear NHERF1 expression levels in the tumors arising from Cav-1 KO mice. The data obtained in the MMTV-neu model are consistent with a role for Cav-1 in adjacent breast cancer stromal cells in modulating the expression and localization of important proteins implicated in tumor cell behavior.","palabras_clave":"CAVEOLIN-1|PTEN|NHERF1|BETA-CATENIN|BREAST CANCER|HER-2\/NEU|ONCOGENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3151,"idioma_id":5,"titulo":"15° BAFICI","resumen":"El artículo ofrece una reseña de la 17° edición de Festival Internacional de Cine Independiente de Buenos Aires (BAFICI), llevado a cabo del 15 al 25 de abril de 2015.","palabras_clave":"RESEÑA|17° BAFICI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3162,"idioma_id":4,"titulo":"CURVED FOSSIL BEE CELLS AS TOOLS FOR RECONSTRUCTING THE EVOLUTIONARY HISTORY AND PALAEOGEOGRAPHICAL DISTRIBUTION OF DIPHAGLOSSINAE (APOIDEA, COLLETIDAE)","resumen":"The new ichnospecies Celliforma curvata isdescribed to include curved fossil bee cells from Argentina,Uruguay and the USA. The upper part of the cell (neck) ofthe new ichnospecies is curved, and accordingly, it can beattributed to bees of the subfamily Diphaglossinae (Colletidae).The oldest record of C. curvata, from the earlyEocene of North America (52?49 Ma), provides a minimumage for the appearance of this subfamily, in accordancewith an already proposed calibrated phylogeny. It isalso proposed that these fossil cells could be used forfuture calibrations of molecular clocks. C. curvata indicatesthat Diphaglossinae had a widespread distribution, fromsouthern Utah to extra-Andean Patagonia at 42°S. In contrast,extant representatives reach only 38°S in this region.Diphaglossinae were more extended southwards in the pastthanks to better environmental conditions in extra-AndeanPatagonia.","palabras_clave":"Celliforma curvata isp. n.|curved bee cells,|Diphaglossinae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3165,"idioma_id":4,"titulo":"Dietary patterns and risk of urinary tract tumours: a multilevel analysis of individuals in rural and urban contexts.","resumen":"  Bladder  cancer is the fourth most frequently diagnosed malignancy in males in Córdoba,  Argentina. The evidence regarding an association between urinary tract tumours  and dietary factors still remains controversial. Argentina has particular  dietary habits, which have already been associated with cancer occurrence. Purposes:  (a) To estimate the association of typical dietary patterns in Argentina on the  occurrence of urinary tract tumours, and (b) to assess the urban-rural context  of residence and cancer occurrence dependency.  Methods: A case-control study of urinary tract  tumours (n=123,  41\/82 cases\/controls) was performed in Córdoba Province (Argentina),  2006-2011. A two-level logistic regression model was fitted, taking into  account rural-urban residence. An exhaustive probabilistic sensitivity analysis  (bias analysis) was performed.  Results:  Southern Cone pattern, characterized by red meat, starchy vegetables and wine consumptions  (OR 1.75 high versus low level of adherence to the pattern), and a medium adherence  to a High-sugar drinks pattern, with high loadings for soft drinks (OR 2.55), were associated with increasing risk  of urinary tract tumours. High adherence to the latter pattern was inversely  associated (OR 0.72). The occurrence of urinary tract tumours was also linked  to place of residence (urban-rural), explaining more than 20% of outcome  variability and improving the above risk estimations. Conclusions: A high  intake of red meat, starchy vegetables and wine, and a moderate intake of  high-sugar drinks seem to be associated with increased risk of urinary tract tumours,  with differences related to the context of residence.  ","palabras_clave":"URINARY TRACT TUMOR|DIETARY PATTERNS|MULTILEVEL MODEL|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3169,"idioma_id":5,"titulo":"ENCIAS SANAS, BEBES SANOS. PARTE II.","resumen":"Se informará cuales son los signos y síntomas de inflamación de las encías ,con el objetivo que al ser reconocidos se transformen en motivo de consulta odontológica. Se resaltará la importancia de la consulta preventiva al comienzo del embarazo. Se organizarán actividades educativas para promover la prevención de enfermedades gingivoperiodontales contribuyendo a su conocimiento en esta población adolescente vulnerable. Se eligió una escuela con localización urbana donde la mayoría de los alumnos provienen de familias de medianos ingresos y con servicios básicos satisfechos.","palabras_clave":"ENFERMEDADES GINGIVOPERIODONTALES|BEBES |PREVENCIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3171,"idioma_id":4,"titulo":"ISCHEMIC POSTCONDITIONING REDUCES INFARCT SIZE THROUGH THE &#945;-1 ADRENERGIC RECEPTOR PATHWAY","resumen":"                The &#945;1-adrenergic  receptors (&#945;1-ARs)  are involved in preconditioning. Given that certain intracellular pathways  appear to be shared by preconditioning and postconditioning, it is possible  that postconditioning could be also mediated by &#945;1-AR. The objective was to evaluate whether &#945;1-AR  activation could trigger postconditioning by analyzing infarct size; and also  determine Akt and GSK-3&#946;  phosphorylation. Langendorff-perfused rat hearts were subjected to 30 minutes  of ischemia and 120 minutes of reperfusion (I\/R; n=8). After 30 min of global  ischemia, we performed 6 cycles of reperfusion\/ischemia of 10 sec each,  followed by 120 min of reperfusion (postcon; n=9). In another postcon group, we  administered prazosin during postcon protocol (postcon + prazosin; n=7). Finally,  we repeated the I\/R group but prazosin (prazosin; n=7), phenylephrine (PE, n=5)  and clonidine (CL, n=6), were administered during the first 2 minutes of  reperfusion. Infarct size was measured using the triphenyltetrazolium chloride  technique, and total and phosphorylated Akt and mitochondrial GSK-3&#946; expression,  were measured by western blot. Infarct size was 58.1±5.1% in I\/R. Postcon and  PE reduced infarct size to 40.1±2.9% and 35.3±5.5 respectively (p&#946;  phosphorylation were higher in the postcon and PE groups compared with the I\/R  and postcon + prazosin groups. Prazosin or clonidine administration did not  modify either protein expression or infarct size. Our data demonstrate that  postconditioning decrease infarct size by activation of the &#945;1-AR pathway  through Akt and GSK-3&#946;  phosphorylation.  ","palabras_clave":"ISCHEMIC POSTCONDITIONING|ALFA-1 ADRENERGIC RECEPTOR|INFARCT SIZE|AKT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3172,"idioma_id":4,"titulo":"Set-up of an infrared fast behavioral assay using zebrafish (Danio rerio) larvae, and its application for high-throughput compound biotoxicity screening.","resumen":"Zebrafish (Danio rerio) is increasingly employed for evaluating   toxicity and drug discovery assays. Commonly experimental approaches   for biotoxicity assessment are based on visual inspection or video   recording. However, these techniques are limited for large-scale assays,   as they demand either a time-consuming detailed inspection of the   animals or intensive computing resources in order to analyze a   considerable amount of screenshots. Recently, we have developed a simple   methodology for tracking the locomotor activity of small animals   cultured in microtiter plates. In this work, we implemented this   automatic methodology, based on infrared (IR) microbeam scattering, for   measuring behavioral activity in zebrafish larvae. We determined the   appropriate culture conditions, number of animals and stage of   development to get robust results. Furthermore, we validated this   methodology as a rapid test for evaluating toxicity. By measuring the   effects of reference compounds on larvae activity, we were able to   estimate the concentration that could cause a 50% decrease in activity   events values (AEC50), showing a strong linear correlation (R2&#8201;=&#8201;0.91) with the LC50 values obtained with the standard DarT test. The toxicity order of the measured compounds was CuSO4&#8201;&gt;&#8201;2,4-dinitrophenol&#8201;&gt;&#8201;3,4-dichloroaniline&#8201;&gt;&#8201;SDS&#8201;&gt;&#8201;sodium benzoate&#8201;&gt;&#8201;EDTA&#8201;&gt;&#8201;K2CrO4;   regarding solvents, EtOH&#8201;&#8776;&#8201;DMSO. In this study, we demonstrate that   global swimming behavior could be a simple readout for toxicity, easy to   scale-up in automated experiments. This approach is potentially   applicable for fast ecotoxicity assays and whole-organism   high-throughput compound screening, reducing the time and money required   to evaluate unknown samples and to identify leading pharmaceutical   compounds","palabras_clave":"behavioral assay|zebrafish (Danio rerio)|compound biotoxicity screening"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3173,"idioma_id":4,"titulo":"Evaluating root-knot nematode infection in wild potatoes","resumen":"Cultivated potato varieties are highly susceptible to root-knot nematodes (Meloidogyne spp.). Resistance in wild relatives could be transferred to breeding lines as an environmentally friendly strategy. We evaluated 28 genotypes of three putatively resistant wild potato species (Solanum spegazzinii, S. kurtzianum and S. vernei) from Argentina for reaction to one of the most damaging potato root-knot nematodes, Meloidogyne arenaria. Two screening assays found great variability among and within species of potato in response to nematode infection. The nematode reproductive efficiency was significantly reduced in two genotypes of S. spegazzinii, which exhibited low to moderate resistance to M. arenaria.","palabras_clave":"CENTRAL WEST ARGENTINA|MELOIDOGYNE|SOLANUM|WILD GERMPLASM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3175,"idioma_id":5,"titulo":"Análisis preliminar de la variabilidad génetica y de los límites específicos en Escallonia alpina-Escallonia rubra (Escalloniaceae).","resumen":"ver proximamente","palabras_clave":"Filogeografia|Escallonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3176,"idioma_id":5,"titulo":"Recursos humanos especializados y cambios tecnológicos en la vitivinicultura de la provincia de Mendoza (Argentina), 1890-1920","resumen":"La conformación de una economía capitalista regional especializada en una vitivinicultura de masa en Mendoza, orientada hacia el mercado nacional, implicó un espectacular cambio técnico que cristalizó, hacia 1890, en cultivos vitícolas de altos rendimientos y en bodegas tecnificadas, de gran capacidad de elaboración, superadoras de viejas tradiciones artesanales. Ya durante la década de 1900 es posible vislumbrar la maduración y adaptación de aquellas tecnologías. En el presente trabajo, analizaremos este último proceso sobre la base, por un lado, de las particulares dinámicas de introducción de innovaciones en las bodegas y, por el otro, de las políticas públicas que tuvieron repercusión en el área tecnológica, específicamente en la formación de recursos humanos (enólogos). Evaluaremos, así, el impacto de estas políticas sectoriales a través de la posterior inserción de aquellos técnicos en las bodegas a partir de 1904, un momento de expansión económica y agroindustrial. Nos interesa conocer, además, los factores contextuales que promovieron, o retardaron, la extensión de esta figura en las bodegas locales.     Nuestra investigación se inscribe en una perspectiva que vincula el estudio de los proyectos educativos de orientación productiva y su aporte a la introducción y difusión de innovaciones y adaptaciones tecnológicas.   ","palabras_clave":"VITIVINICULTURA|TECNOLOGIAS|REC URSOS HUMANOS|CONOCIMIENTOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3177,"idioma_id":5,"titulo":"Consideraciones sobre la presencia y el estado actual del psicoanálisis universitario en París: psicopatología, metapsicología y clínica en la enseñanza del psicoanálisis. I. La enseñanza del psicoanálisis y su inserción en las universidades","resumen":"En el artículo se examina la expandida categoría 'états limites' (estados fronterizos) en determinado ámbito del psicoanálisis universitario francés, situado especialmente en referencia a las enseñanzas impartidas en las universidades Paris V y VII. Se analizan diversos esbozos teóricos en relación con este cuadro, en función de las complejas problemáticas que supone su planteo, tanto desde el punto de vista metapsicopatológico como psicopatológico y clínico.","palabras_clave":"ESTADOS LÍMITES\/ FRONTERIZOS|PSICOPATOLOGÍA|METAPSICOLOGÍA|CLÍNICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3178,"idioma_id":4,"titulo":"Mathematical Model for the Bulk Polymerization of Styrene Chemically Initiated by Sequential and Total Decomposition of the Symmetrical Cyclic Trifunctional Initiator Diethyl Ketone Triperoxide","resumen":"This work experimentally and theoretically investigates the use of the symmetrical cyclic trifunctional initiatordiethyl ketone triperoxide (DEKTP) in the bulk polymerization of styrene (St). The study focused on temperatures of 150 to 200C, considering chemical initiation by both sequential and total decomposition reactions. The experimental work consisted of a series of isothermal batch polymerizations at higher temperatures, 150 and 200C, with an initiator concentration of 0.01 mol\/L. The mathematical model is based on a kinetic mechanism that includes thermal and chemical initiation (both sequentialand total decomposition reactions), propagation, transfer to monomer, termination by combination and re-initiation reactions. Experimental and theoretical results show thatthe decomposition mechanism of the initiator is modified by the reaction temperature and can be modeled as a set of two parallel reactions with different temperature dependences. The developed mathematical model simulates the bulk polymerization of St in the presence of DEKTP for a wide temperature range (120?200C). It was found that due to these two decomposition mechanisms, the system may behave as a ?dead-end? polymerizationsystem above a certain temperature, yielding low molecular weights and a limiting conversion value. Simulation results indicate the value of this temperature to be about185C.&nbsp;","palabras_clave":"Polystyrene|Mathematical Modelling|Diethyl Ketone Triperoxide|Molecular Structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3179,"idioma_id":4,"titulo":"Intraspecific Variation and Erythrocyte sizes of six populations of Hypsiboas cordobae (Anura, Hylidae).","resumen":"We studied the morphology and size of erythrocytes of H. cordobae, and analysed the geographic variationof this character along the distribution of the species, in relation to the latitudinal and altitudinal distances. Erythrocyteshape of the H. cordobae is ellipsoidal and the nuclei are also ellipsoidal and centrally oriented. Erythrocyteand nuclear size showed significant differences among populations, with the highest mean size corresponding to thepopulation of Achiras (low altitude site) and the lowest mean size to Los Linderos (high altitude site). There was nosignificant relationship between the latitude of each population and the both erythrocyte and nuclear size. The altitudinalvariation in erythrocyte cell size may be attributable to the surface available for gas exchange; a small erythrocyteoffers a possibility of greater rate of exchange than a larger one. Our results are consistent with studies of otheramphibians, where intraspecific comparisons of populations at different altitudes show that individuals at higher altitudesare characterized by smaller erythrocytes.","palabras_clave":"Erythrocyte sizes |Cordoba|Hypsiboas cordobae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3181,"idioma_id":4,"titulo":"Scope and Limits of  \u201CGeneral\u201D And \u201CDescriptive\u201D Legal Theories","resumen":"The purpose of this essay is to shed some light on general and descriptive theories of law ? like the one proposed by H.L.A. Hart. To achieve such a goal, we will highlight some of the hurdles that these theories face by: i) trying to identify and adequately explain the necessary features of a practice that it considers absolutely contingent; ii) proposing a general theory that explains only our concept of the law; iii) developing a theory that, in spite of being non-evaluative,  complies with a set of values; iv) describing a normative practice; etc. Finally, this essay will present some issues that call for new and better arguments to defend descriptive theories of law which attempt to offer a general explanation of law without making evaluative judgments.","palabras_clave":"Legal Philosophy|Jurisprudence|Normativity|Positivism|H. L. A. Hart"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3182,"idioma_id":4,"titulo":"Lactosylated poly(ethylene oxide)-poly(propylene oxide) block copolymers for potential active drug targeting: Synthesis and physicochemical and self-aggregation characterization","resumen":"Aiming to develop polymeric self-assembly nanocarriers with potential applications in active drug targeting to the liver, linear and branched poly(ethylene oxide)- poly(propylene oxide) (PEO-PPO) amphiphiles were conjugated to lactobionic acid (LA), a disaccharide of galactose and gluconic acid, by the conventional Steglich esterification reaction. The conjugation was confirmed by ATR\/FT-IR, 1H-NMR and 13C-NMR spectroscopy. MALDI-TOF mass spectrometry and elemental analysis were employed to elucidate the conjugation extent and the final molecular weight,  respectively. The critical micellar concentration (CMC), the size and size distribution and zeta potential of the pristine and modified polymeric micelles under different conditions of pH and temperature were characterized by dynamic light scattering (DLS). Conjugation with LA favored the micellization process, leading to a decrease of the CMC with respect to the pristine counterpart, this phenomenon being independent of the pH and the temperature. At 37ºC, micelles made of pristine copolymers showed a monomodal size distribution between 12.8 and 24.4 nm. Conversely, LA-conjugated micelles showed a bimodal size pattern that comprised a main fraction of relatively small size (11.6-22.2 nm) and a second one with remarkably larger sizes of up to 941.4 nm. The former corresponded to single micelles, while the latter would indicate a secondary aggregation phenomenon. The spherical morphology of LA-micelles was visualized by transmission electron microscopy. Finally, to assess the ability of the LAconjugated micelles to interact with lectin-like receptors, samples were incubated with concanavalin A at 37ºC and the size and size distribution were monitored by DLS. Findings indicated that regardless of the relatively weak affinity of this vegetal lectin for galactose, micelles underwent agglutination probably through the interaction of a secondary site in the lectin with the gluconic acid unit of LA.","palabras_clave":"Poly(ethylene oxide)-poly(propylene oxide) polymeric micelles|Lactobionic acid-conjugated poloxamers and poloxamines|Steglich esterification|Lectin-like receptors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3183,"idioma_id":5,"titulo":"AYLACOSTOMA  STIGMATICUM  (HYLTON  SCOTT,  1954):  ANTECEDENTES  DE  LA  ESPECIE.","resumen":"Aylacostoma  stigmaticum fue  descripta  por  Hylton  Scott  como  Aylacostoma stigmatica.  La  terminación  de  la  especie  fue   considerada originalmente como femenina  y fue corregida por Cazzaniga (1992), en concordancia con  la  terminación  -stoma del  género  que  es   neutra.  Martín  &amp;  César  (2004)  mencionan  el nombre  Hemisinus  stigmaticus,  pero  éste  nunca fue publicado como nombre válido.","palabras_clave":"Molusco|Aylacostoma|Conservación|Extinta"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3185,"idioma_id":4,"titulo":"Solid-phase Petasis multicomponent reaction for the generation of &#946;-lactams 3-substituted with non-proteinogenic &#945;-amino acids","resumen":"An efficient protocol for the rapid generation of libraries of biologically promising b-lactams is described.  Key step is a solid-phase based multicomponent Petasis reaction","palabras_clave":"Solid-phase organic synthesis|Multicomponent reaction|Petasis reaction|beta-lactam"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3186,"idioma_id":5,"titulo":"INFLUENCIA DE CONTAMINACIONES CAUSADAS POR MICROORGANISMOS OPORTUNISTAS EN RATONES DE LA CEPA BALB\/c.Fox1nu TRANSPLANTADA CON LA LÍNEA TUMORAL HUMANA A549","resumen":"Entre las cepas de ratones inmunodeficientes se encuentra la BALB\/c.Fox1nu la cual se utiliza como modelo animal para el transplante de tumores humanos. Estos ratones se producen bajo estrictas barreras sanitarias y deben estar libres de sus patógenos específicos; entre ellos, los oportunistas más frecuentes son Pseudomonas aeruginosa, Klebsiella oxytoca, Proteus mirabilis y Citrobacter spp. El objetivo de este trabajo fue evaluar la interferencia que causan los agentes patógenos oportunistas Pseudomonas aeruginosa, Klebsiella oxytoca, Proteus mirabilis y Citrobacter spp. en ratones BALB\/c.Fox1nu trasplantados con la línea tumoral humana A549. Cien ratones hembras BALB\/c.Fox1nu de 4 a 6 semanas se dividieron en diez grupos (G) de 10 animales cada uno: G1, transplantados con la línea tumoral A549; G2 inoculados con Pseudomonas aeruginosa; G3, inoculados con Klebsiella oxytoca; G4, inoculados con Proteus mirabilis; G5, inoculados con  Citrobacter spp, G6 transplantados con la línea tumoral e inoculados con Pseudomonas aeruginosa, G7 transplantados con la línea tumoral e inoculados con Klebsiella oxytoca, G8 transplantados con la línea tumoral e inoculados con Proteus mirabilis, G9 trasplantados con la línea tumoral e inoculados con Citrobacter spp. y G10 control. Siete de los 10 animales del G1 presentaron crecimiento tumoral, los ratones de los G2 al G 5 no mostraron signos clínicos; los animales de los G6 al G9 no mostraron signología clínica y el desarrollo tumoral se comportó como en los 7 ratones del grupo el G1; a la necropsia se aisló Pseudomonas aeruginosa, Klebsiella oxytoca, Proteus mirabilis y Citrobacter spp. en los animales del G2 al G9 respectivamente; el G10 no presentó lesiones ni se aíslo el microorganismo. Se concluyó que las infecciones por estos patógenos oportunistas no son fatales en ratones BALB\/c.Fox1nu y no interfieren en el desarrollo del tumor.","palabras_clave":"Contaminaciones|Tumoral|ratones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3187,"idioma_id":4,"titulo":"6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells","resumen":"Abstract  BACKGROUND:  IL-&#948; (5-hydroxy-6 iodo-8,11,14-eicosatrienoic delta lactone) an iodinated arachidonic acid (AA) derivative, is one of the iodolipids biosynthesized by the thyroid. Although IL-&#948; regulates several thyroid parameters such as cell proliferation and goiter growth it was found that this iodolipid inhibits the growth of other non thyroid cell lines.  OBJECTIVES:  To study the effect of IL-&#948; on cell proliferation and apoptosis in the colon cancer cell line HT-29.  RESULTS:  Treatment with IL-&#948; reduced cell viability in a concentration-dependent manner: 1&#956;M 20%, 5&#956;M 25%, 10&#956;M 31%, 50&#956;M 47% and caused a significant decrease of PCNA expression (25%). IL-&#948; had pro-apoptotic effects, evidenced by morphological features of programmed cell death such as pyknosis, karyorrhexis, cell shrinkage and cell blebbing observed by fluorescence microscopy, and an increase in caspase-3 activity and in Bax\/Bcl-2 ratio (2.5 after 3h of treatment). Furthermore, IL-&#948; increased ROS production (30%) and lipid peroxidation levels (19%), suggesting that apoptosis could be a result of increased oxidative stress. A maximum increase in c-fos and c-jun protein expression in response to IL-&#948; was observed 1h after initiation of the treatment. IL-&#948; also induced a tumour growth delay of 70% compared to the control group in NIH nude mice implanted with HT-29 cells.  CONCLUSION:  Our study shows that IL-&#948; inhibits cell growth and induces apoptosis in the colon cancer cell line, HT-29 and opens the possibility that IL-&#948; could be a potential useful chemotherapy agent.","palabras_clave":"iodolipids|iodolactone|arachidonic acid|colon cancer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3189,"idioma_id":4,"titulo":"A COMPARATIVE PHOTOCHEMICAL STUDY ON THE BEHAVIOUR OF 3,3&#769;- DIHYDROXYFLAVONE AND ITS COMPLEX WITH LA(III) AS GENERATORS AND QUENCHERS OF REACTIVE OXYGEN SPECIES","resumen":"A 1:1 COMPLEX BETWEEN 3,3 DIHYDROXYFLAVONE AND LA (III) IS FORMED IN METHANOLIC SOLUTION. THE PHOTOPHYSICAL PROPERTIES OF THE COMPLEX AND THE FREE FLAVONOID ARE DISCUSSED IN COMPARISON TO THE WELL KNOWN RELATED COMPOUND 3=HYDROXYFLAVONE.A DETAILED KINETICS AND MECHANISTIC STUDY EMPLOYING POLAROGRAPHIC MONITORING OF OXYGEN UPTAKE AND TIME RESOLVED DETECTIION OF O2     (1DELTAg) PHOSPHORESCENCE INDICATES THAT DHF AND THE COMPLEX REACT WITH  O2 (1DELTAg) AND O2.= BY A NON SIMPLE MECHANISM.IT WAS FOUND THAT NETAL CHELATION GREATLY ENHANCES THE ABILITY OF DHF AS AN OVERALL O2 (1DELTAg)QUENCHER.","palabras_clave":"3,3´-DIHYDROXYFLAVONES - LA(III) COMPLEXES|PHOTODEGRADATION|PHOTOSENITIZATION|REACTIVE OXYGEN ESPECIES|RYBOFLAVIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3190,"idioma_id":4,"titulo":"pQCT assessed relationships between diaphyseal design and cortical bone mass and density in the tibiae of healthy sedentary and trained men and women","resumen":"  In a pQCT study of running-trained and untrained men and women we had shown that bone mass distribution along the tibia was adapted to the usage-derived stress pattern. To study the possible association between the efficiency of diaphyseal design and bone material stiffness, we extend the analysis of the same sample to correlate pQCT indicators of the distribution (CSMIs), mass (BMC), and density (vBMD) of cortical bone tissue as descriptors of ?distribution\/mass? (d\/m) or ?distribution\/quality? (d\/q) relationships. The d\/m and d\/c curves followed positive (exponential) and negative (hyperbolic-like) equations, respectively. Distribution curves of r coefficients throughout the bone were all bell-shaped, reaching a maximum towards the mid-diaphysis.The CSMIs and BMC were higher, and vBMD was lower in men than women and in runners than non-runners. The d\/m relationships were described by unique curves for all groups while d\/q relationships were better adjusted to separate curves for men and women. Results support that: 1. diaphyseal design reflects the relative influence of bending\/torsion stress along the bones, tending to minimize bone mass; 2. there is a trade-off between cortical bone ?quality? and distribution; 3. d\/m and d\/q relationships are related to bone mechanical environment, and 4. d\/q relationships are affected by sex.","palabras_clave":"Biomecánica ósea|Trained people|Osteoporosis|pQCT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3191,"idioma_id":4,"titulo":"Mathematical modelling of momentun, heat and mass transfer in grains stored in silos. Part I: Model development and validation","resumen":"A bidimensional finite element model that predicts temperature distribution, moisture migration and natural convection currents in stored grain is described. Validation was performed by comparing predicted and measured variables under laboratory conditions in corn and wheat. On average, standard errors were at most 1.7oC for temperature and 0.69 %w.b for moisture content. The sensitivity of the solution to variations in grain hygroscopic properties and to election of a sorption isotherm equation was examined. For a given temperature gradient, moisture migration was higher in soybean than in corn and wheat. As result of hygroscopic properties and permeability of grain, wheat exhibited the highest resistance to moisture migration","palabras_clave":"grain storage|numerical modelling|natural convection|heat and mass transfer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3192,"idioma_id":4,"titulo":"THE GEOMETRY OF THE ACTIVE STRIKE-SLIP EL TIGRE FAULT, PRECORDILLERA OF SAN JUAN, CENTRAL-WESTERN ARGENTINA: INTEGRATION OF RESISTIVITY SURVEYS WITH STRUCTURAL AND GEOMORPHOLOGICAL RESEARCH","resumen":"The geometry and related geomorphological features of the right-lateral   strike-slip El Tigre Fault, one of the main morphostructural   discontinuities in the Central-Western Precordillera of Argentina, were   investigated. Achievements of this survey include: recognition of   structural and geometrical discontinuities along the fault trace,   identification and classification of landforms associated with local   transpressional and transtensional sectors, observation of significant   changes in the fault strike and detection of right and left bends of   different wavelength. In the Central Segment of the El Tigre Fault, 2D   electrical resistivity tomography surveys were carried out across the   fault zone. The resistivity imaging permitted to infer the orientation   of the main fault surface, the presence of blind fault branches along   the fault zone, tectonic tilting of the Quaternary sedimentary cover,   subsurface structure of pressure ridges and depth to the water table.   Based on this information, it is possible to characterize the El Tigre   Fault also as an important hydro-geological barrier. Our survey shows   that the main fault surface changes along different segments from a   high-angle to a subvertical setting whilst the vertical-slip component   is either reverse or normal, depending on the local transpressive or   transtensive regime induced by major bends along the trace. These local   variations are expressed as sections of a few kilometres in length with   relatively homogeneous behaviour and frequently separated by oblique or   transversal structures.","palabras_clave":"EL TIGRE FAULT|ARGENTINE PRECORDILLERA|NEOTECTONICS|FAULT STRUCTURE|GEOMORPHOLOGY|2D ELECTRICAL RESISTIVITY TOMOGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3193,"idioma_id":4,"titulo":"Search for long-lived, multi-charged particles in pp collisions at &#8730;s=7 TeV using the ATLAS detector","resumen":"A search for highly ionising, penetrating particles with electric charges from |q|=2e|q|=2e to 6e is performed using the ATLAS detector at the CERN Large Hadron Collider. Proton\u2013proton collision data taken at s=7&nbsp;TeV during the 2011 running period, corresponding to an integrated luminosity of 4.4&nbsp;fb&#8722;14.4&nbsp;fb&#8722;1, are analysed. No signal candidates are observed, and 95% confidence level cross-section upper limits are interpreted as mass-exclusion lower limits for a simplified Drell\u2013Yan production model. In this model, masses are excluded from 50&nbsp;GeV50&nbsp;GeV up to 430, 480, 490, 470 and 420&nbsp;GeV420&nbsp;GeV for charges 2e, 3e, 4e, 5e and 6e, respectivel","palabras_clave":"search|long-lived|multi-charged|atlas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3194,"idioma_id":4,"titulo":"Production of ultrapure hydrogen in a Pd-Ag membrane reactor using noble metals supported on La-Si oxides. Heterogeneous modeling for the water gas shift reaction","resumen":"Two different catalysts, Rh(0.6% wt\/wt)\/La2O3(27% wt\/wt)·SiO2 and Pt(0.6% wt\/wt)\/La2O3(27%)·SiO2, were tested in the WGS reaction. Their performances were first studied in a conventional fixed-bed reactor. Their activities were similar and they were both very stable. However, as Pt(0.6)\/La2O3(27)·SiO2 showed a much higher selectivity to the desired reaction, the performance of a membrane reactor employing this catalyst was studied. The effects of the H2O\/CO ratio, space velocity, sweep gas flow rate and size of the catalyst particle on CO conversion and H2 recovery were studied at laboratory scale under isothermal conditions. A 1-D heterogeneous model was developed in order to properly reproduce the experimental results obtaining good agreement between the simulation results and laboratory data. The experimental and theoretical results confirm the existence of significant external mass-transfer limitations in the fluid-particle interface for these very active formulations.","palabras_clave":"H2 PURIFICATION |H2 PRODUCTION|Pt CATALYSTS|MASS TRANSFER LIMITATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3195,"idioma_id":5,"titulo":"Rol de la isoforma 2 de la glicerol-3-fosfato aciltransfersa 2 en el metabolismo lipídico testicular","resumen":"         El primer paso en la síntesis de novo de glicerolípidos es la acilación del glicerol-3-fosfato, la cual es catalizada por la enzima glicero-3-fosfato aciltransferasa (GPAT). En mamíferos han sido descriptas hasta el momento cuatro isoformas de GPAT, las cuales pueden diferenciarse por su localización tisular y subcelular, y por su sensibilidad a reactivos que reaccionan con los grupos sulfhidrilos. El objetivo de este trabajo fue estudiar la localización y función de GPAT2, una isoforma mitocondrial que se expresa principalmente en testículo utilizando como modelo células CHO-K1 que sobreexpresan GPAT2. Se observó que esta isoforma utiliza como sustrato específicamente al ácido araquidónico, estimula la síntesis de triacilgliceroles (TAG) con alto contenido de ácido araquidónico, y favorece la incorporación de [1-14C] ácido araquidónico en los TAG. Utilizando modelos animales se observó por un lado que el ARNm de GPAT2 se expresa sólo en los espermatocitos primarios, mientras que la expresión de la proteína se mantiene a lo largo de la espermatogénesis; y por otro lado que la expresión de GPAT2 varía durante el desarrollo sexual, alcanzando un máximo de expresión y actividad a los 30 días de edad en la rata. A esta edad se observó también un máximo en el contenido de TAG y de AA esterificado en los TAG del testículo. Se concluye que los resultados sugieren que GPAT2 sería la enzima responsable de esterificar el ácido araquidónico en los TAG de las células de la línea espermática.","palabras_clave":"TESTICULO|GPAT|MITOCONDRIA|TRIACILGLICEROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3196,"idioma_id":4,"titulo":"The Tumor Microenvironment: Characterization, Redox Considerations, and Novel Approaches for Reactive Oxygen Species-Targeted Gene Therapy","resumen":"The tumor microenvironment is a complex system that involves the  interaction between malignant and neighbor stromal cells embedded in a  mesh of extracellular matrix components. Stromal cells (fibroblasts,  endothelial and inflammatory cells) are co-opted at different stages to  help malignant cells invade surrounding extracellular matrix and  disseminate. Malignant cells have developed adaptive mechanisms to  survive under the extreme conditions of the tumor microenvironment such  as restricted oxygen supply (hypoxia), nutrients deprivation and a  prooxidant state among others. These conditions could be eventually  used to target drugs that will be activated specifically in this  microenvironment. Preclinical studies have shown that modulating  cellular\/tissue redox state by different gene therapy approaches were  able to control tumor growth. In this review we describe the most  relevant features of the tumor microenvironment, addressing reactive  oxygen species-generating sources that promote a prooxidative  microenvironment inside the tumor mass. We describe different gene  therapy approaches that promote either decreased or exacerbated  prooxidative microenvironment, and those that make use of the  differential levels of ROS between cancer and normal cells to achieve  tumor growth inhibition.","palabras_clave":"TUMOR MICROENVIRONMENT|GENE THERAPY|REACTIVE OXYGEN SPECIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3197,"idioma_id":4,"titulo":"USE OF BIOSTIMULANTS IN CROPS. I SOYBEAN","resumen":"The biostimulants are natural or synthetic compounds that can be  applied to plants (leaves, fruits, seeds) causing changes in  physiological and structural processes in order to improve the  physiological quality and the crop establishment and grain yield.  The aim of this study was evaluate the effect of biostimulant  Stimulate®, which formulation includes 0.005% indol butyric acid  (auxin), 0.005% gibberellic acid and 0.009% kinetin (cytokinin),  applied to the seed and leaf, on the soybean crop establishment and  yield (Maturity Group MG V) in Rio Cuarto. Treatments were 5:  Stimulate® applications on seeds (300 ml\/100 kg) and foliage (250  ml\/ha) in R1 and R3 stages, their combinations, and a control during  the 2012\/13 growing season. The seed coating significantly increased  main root length, secondary roots and leaves unfolded number at 15  and 32 days after sowing. At harvest the crop showed higher biomass  per plant and increase the fruits and seeds number and weight (per  plat and area), and grain yield when biostimulant was applied on  seeds, without effect of foliage application in reproductive stages  or interaction between the types of applications. The changes found  in grain yield are mainly due to the number of seeds per area,  without significant effects on the individual seed weight and harvest  index","palabras_clave":"PHYSYIOLOGICAL QUALITY|ROOT|SEED"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3198,"idioma_id":5,"titulo":"ESTUDIO MORFOLOGICO POR MICROSCOPIA ELECTRONICA DE BARRIDO DE Mycoplasma spp. DE ORIGEN  BUCAL ADHERIDO A SUPERFICIE BIOCOMPATIBLE","resumen":"Los micoplasmas son microorganismos carentes de pared celular y  se caracterizan por ser los más pequeños capaces de replicarse por sí mismos.  La placa dental alberga una amplia variedad de microorganismos diferentes    Varios investigadores demostraron la presencia del género Mycoplasma en  placa dental asociadas con enfermedad periodontal.   Los microorganismos residentes en la placa dental pueden acceder por diferentes maniobras odontológicas al torrente circulatorio e involucrarse  en la infección de prótesis cardio-vasculares ocasionando su posterior  recambio.  El nitinol es un material  utilizado especial en la confección de prótesis cardiovasculares.  El objetivo del presente trabajo fue estudiar por microscopía electrónica de barrido (MEB) la morfología del género Mycoplasma  adherido  a superficies de nitinol.   Métodos: La cepa de Mycoplasma utilizada fue aislada de placa subgingival de un sitio con periodontitis crónica.  Veinticinco superficies de nitinol fueron suspendidas  en forma individual en caldo PPLO estéril suplementado con suero y extracto de levadura inoculado con una suspensión de Mycoplasma sp. e incubada en una atmósfera de 90% de H 2 y 10% de CO2 a 37º C. durante 48 hs.   Al cabo de dicho lapso las muestras fueron  lavadas repetidas veces con agua destilada estéril y fijadas en formol al 10% durante 24 hs., deshidratadas en alcoholes ascendentes y metalizadas para su posterior visualización por microscopia electrónica de barrido (Microscopio FEI Quanta 200). Se realizaron los controles respectivos.   Resultados: La observaciones por microscopia electrónica de barrido demostraron la adhesión del Mycoplasma sp. a las superficies de nitinol en la totalidad de las muestras estudiadas.   Los micoplasmas se observaron con su pleomorfismo característico en formas cocoideas, cocobacilares, bacilares, acampanadas o pirifomes, en anillos y filamentosas, estas últimas en ocasiones con ramificaciones.También se observaron células micoplamales en estado de reproducción.  Los controles respectivos de superficies de nitinol sin inocular no presentaron formas compatibles con micoplasmas.  Conclusiones: Debido a lo expuesto se concluye que el género Mycoplasma  de origen bucal adherido a superficies de nitinol presenta el pleomorfismo característico de otras especies de su género.","palabras_clave":"MYCOPLASMA|PLACA DENTAL|NITINOL|ADHESION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3199,"idioma_id":4,"titulo":"Acute effects of third generation &#946;-blockers on short-term and beat-to-beat blood pressure variability in sinoaortic-denervated rats.","resumen":"An increase in blood pressure variability (BPV) contributes to the development of target organ damage associated with  hypertension. Treatment with conventional b-blockers, such as atenolol, has been associated with an increase in BPV; however,  the extrapolation of these results to third generation b-blockers with pleiotropic effects seems to be inappropriate. The  cardiovascular effects of third generation b-blockers, carvedilol and nebivolol, were assessed in sinoaortic-denervated rats (SAD)  and compared with the second generation b-blocker atenolol and the calcium channel blocker verapamil, with a special focus  on short-term BPV. Male SAD rats were acutely treated with carvedilol, nebivolol, atenolol or verapamil at two different doses,  and the effects on blood pressure and BPV were recorded. Short-term BPV was assessed by the s.d. of BP recordings. Beat-tobeat  BPV was studied using spectral analysis to assess the vascular sympatholytic activity of carvedilol and nebivolol by  estimating the effects of these drugs on the ratio of low frequency (LF) to high frequency (HF) BPV (LF\/HF ratio). Nebivolol,  carvedilol and the calcium channel blocker verapamil significantly attenuated short-term BPV at both doses in SAD animals,  and there were no differences between the drugs. Conversely, atenolol did not modify baseline s.d. values at either dose.  Carvedilol and nebivolol significantly reduced the LF\/HF ratio in SAD rats compared with the effects of atenolol and verapamil,  suggesting the ability of the third generation b-blockers to reduce vascular sympathetic activity. In conclusion, third generation  b-blockers induce a marked reduction in short-term BPV in SAD rats compared to atenolol. Moreover, the ability of carvedilol  and nebivolol to reduce short-term BPV in SAD rats is equivalent to that of verapamil, suggesting that these b-blockers may  have an additional beneficial effect through their control of short-term variability to a similar extent to calcium channel  blockers.","palabras_clave":"blood pressure variability|calcium channel blockers|sinoaortic denervation|beta- blockers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3201,"idioma_id":4,"titulo":"Antioxidant defenses and lipid peroxidation in two species of Lithodids from South Atlantic during summer.","resumen":"In Southern South America Lithodes santolla and L. confundens are the most valuable king crabs species, speciallydue to their high yields and their high acceptance in the markets. From a physiological point of view, reactive oxygenspecies (ROS) are continuously produced as by-products of cell respiration. These ROS are transformed into less toxicmetabolites by antioxidant enzymes. The aim of the present study was to determine in Lithodes santolla (from San JorgeGulf) and L. confundens (from Atlantic coast of Tierra del Fuego) the baseline status of both antioxidant enzyme activity andlipid peroxidation levels. Activities of catalase (CAT), glutathione-S-transferase (GST), as well as lipid peroxidation weremeasured in the muscle, gills and hepatopancreas. Both species showed antioxidant enzyme activities, and the gills werethe organs with the highest CAT enzyme activities. CAT and GST activities were higher in muscle and gills, respectively, inL. confundens compared with L. santolla, whereas lipid peroxidation was low in both organs. These differences betweenboth species suggest that L. confundens is more capable to afford oxidative stress than L. santolla, which may be a responseto the common occurrence of L. confundens at the intertidal during the spring low tides. Differences in the analysedparameters could be attributed to intrinsic adaptation features of each king crab species and\/or to environmentalconditions of each particular study area. Furthermore, our results showed that parameters as CAT, GST and lipid peroxidationcould be proposed as biomarkers of oxidative stress in Lithodes santolla and L. confundens.","palabras_clave":"actividad enzimática|especies reactivas del oxigeno|centollas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3202,"idioma_id":5,"titulo":"Referencias a cuestiones ambientales en las normas internacionales de información financiera: Marco Conceptual y Bienes de Uso","resumen":"En el ámbito organizacional la mayoría de las decisiones se toman en situaciones de incertidumbre, constantemente se producen situaciones contingentes e inciertas que luego se resuelven de distintas maneras y que originan efectos en los resultados y en el patrimonio de la empresa, como -por ejemplo- los riesgos asociados a la  contaminación ambiental que la organización causa.    En ciertos casos, los efectos de estos hechos son tan importantes que pueden variar y hasta modificar la situación económico?financiera de la empresa, por tal motivo se los debe considerar al momento de preparar la información contable a incluir en los comúnmente llamados informes contables.  Como es de público conocimiento, a fines del año 2009, la Comisión Nacional de Valores (CNV) en un proyecto conjunto elaborado con la FACPCE (Federación Argentina de Consejos Profesionales en Ciencias Económicas), estableció la aplicación obligatoria de las normas internacionales de información financiera (NIIF) para las empresas emisoras de títulos con cotización en el mercado de valores, y para aquellas sociedades sin cotización pública sobre las cuales ejerzan control o influencia significativa.  Dicha obligación resultó finalmente aplicable  en los estados financieros de ejercicios iniciados a partir del 1º de enero de 2012, surge en la misma norma la posibilidad de aplicación voluntaria de las mismas para aquellas empresas no contenidas entre las mencionadas en este párrafo.   El trabajo, continuación del publicado en el mes de diciembre 2012, comenta sintéticamente algunos aspectos a considerar frente a situaciones ambientales contingentes e inciertas, a fin de registrar su impacto en la información económico-financiera contenida en los informes contables, según la Norma Internacional de Contabilidad (NIC) 37 emitida por el IASB referida a provisiones y a la información sobre activos contingentes y pasivos contingentes; dicha norma incluye referencias expresas para la constitución de provisiones medioambientales.","palabras_clave":"CONTABILIDAD AMBIENTAL|NORMAS INTERNACIONALES DE INFORMACIÓN FINANCIERA|CONTINGENCIAS|CONTABILIDAD INTERNACIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3207,"idioma_id":4,"titulo":"BioSmalltalk: A pure object system and library for bioinformatics.","resumen":"Summary: We have developed BioSmalltalk, a new environment system for pure object-oriented bioinformatics programming. Adaptive end-user programming systems tend to become more important in the discovering of biological knowledge, as the emergence of open-source programming toolkits for bioinformatics have demonstrated in the last years. Our software is intended to bridge the gap between bioscientists and rapid software prototyping while preserving possibility of scaling to whole systems biology applications. This library will facilitate access to popular bioinformatics data formats, services and applications by unexperienced developers. Our software is cross-platform and freely available (MIT license) through the Google Project Hosting environment at   http:\/\/code.google.com\/p\/biosmalltalk","palabras_clave":"BIOINFORMATICS|SEQUENCE ANALYSIS|PROGRAMMING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3253,"idioma_id":5,"titulo":"Identidad y sentido de pertenencia barrial respecto a los límites político-administrativos vigentes. Caso Villa Luro","resumen":"El barrio de Villa Luro, perteneciente a la Comuna 10 de la Ciudad Autónoma de Buenos Aires, presenta la particularidad de incluir un eje este-oeste, generado por barreras viales, que ha determinado una sectorización en sentido norte-sur reforzada por centralidades en sus extremos opuestos, problemática que fundamenta el proyecto de investigación denominado ´Diagnóstico sobre incidencia de las vías de comunicación terrestres, como barreras o centralidades, en la sectorización de barrios. Caso Villa Luro´.Identidad y sentido de pertenencia de vecinos y su relación con los límites político-administrativos vigentes es el objetivo particular a alcanzar mediante el reconocimiento de la situación actual.A través de un desarrollo que indique datos histórico-urbanísticos fundacionales y situaciones actuales surgidas de la interpretación de documentación gráfica oficial y trabajos de campo se pretende alcanzar la elaboración de conclusiones.Una primera encuesta básica, realizada como ejercitación en la asignatura Diseño Arquitectónico, Planeamiento y Urbanismo II, 5to año, Ingeniería Civil, UTN.BA, arroja resultados que validan de alguna manera la desvinculación entre sentido de pertenencia barrial y límites político-administrativos de Villa Luro.Es intención, mediante este trabajo, mostrar divergencias existentes entre la conformación de límites político-administrativos y aquellos que son reconocibles por sus vecinos, entendiéndose que la identidad y sentido de pertenencia barrial no surge luego de la determinación de los límites político-administrativos, según criterios de gobierno, sino por relaciones humanas y circunstancias temporarias que hacen a la conformación de espacios sociales que se materializan en espacios urbanos denominados, barrios.","palabras_clave":"BARRERA|BARRIOS|LÍMITES|SECTORIZACIÓN|VINCULACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3268,"idioma_id":4,"titulo":"Effect of metals on Daphnia magna and cladocerans representatives of the Argentinean Fluvial Littoral","resumen":"Chronic toxicity tests were conducted to assess the effect of Cu, Cr and Pb on Moinodaphnia macleayi andCeriodaphnia dubia -two cladoceran species from the Argentinian Fluvial Littoral Zone (AFLZ)- andDaphnia magna -an holarctic species-. The specimens were exposed to three concentrations of eachmetal. As endpoints, the number of living and dead organisms, molts, neonates released, and the age offirst reproduction were recorded. Chronic assays showed that Cu significantly affected the analyzed lifehistory traits in the three species. The lowest Pb and Cr concentrations did not affect survival, molting orfecundity in D. magna. Conversely, in M. macleayi and C. dubia, survival, molting and fecundity showedhighly significant differences in all the concentrations tested compared to control assay. The present studystresses the importance of using biological parameters as bioindicators, as well as the study species from the Southern Hemisphere to assess metal pollution.","palabras_clave":"MOINODAPHNIA MACLEAYI|CERIODAPHNIA DUBIA|DAPHNIA MAGNA|METAL POLLUTION|LIFE HISTORY TRAITS|BIOINDICATORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3279,"idioma_id":4,"titulo":"Patterns in community assemblages and species richness of small mammals across an altitudinal gradient in semi-arid Patagonia, Argentina","resumen":"This is the first study that explored the altitudinal gradient in Argentine Patagonia. The purpose of this  study was to document the effects produced by altitudinal gradients in the arid lands on the community  composition, abundance, and species richness of small mammals. Four hypotheses of factors that influence  the patterns of diversity proposed for mountain regions were assessed for Patagonian arid lands:  reduction in the effective area, changes in biotic and abiotic parameters, isolation of montane communities  and feedback between zonal communities. The study was carried out in the Somuncurá Protected  Natural Area (500-1600 m a.s.l.), a basaltic plateau from northern Extrandean Patagonia. Mammals were  sampled along altitudinal and vegetation gradients. With at least 20 small mammal species, Somuncurá  is the place with the uppermost species richness in Patagonia. Small mammal abundance and species  richness peaked at intermediate levels (750-1000 m a.s.l.), following a hump-shaped pattern. Temperature  is the main factor to the structuring of small mammal communities in the arid lands and is the  reason why height and latitude have a similar effect in the species composition. The same spatial pattern  can be tracked throughout past climatic changes. Somuncurá should be considered at a higher priority  level for conservation.","palabras_clave":"Altitudinal surveys|Biogeographical affinities|Rodents and opossums|Temperature|Volcanic plateaus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3280,"idioma_id":5,"titulo":"Acerca del concepto de mundo de la vida: Una aproximación desde el Trabajo Social","resumen":"La expresión mundo de la vida (lebenswelt) fue acuñada por Edmund Husserl en su obra póstuma La crisis de las ciencias europeas y la fenomenología trascendental. Este concepto es clave para comprender las implicancias que tiene el Trabajo Social en el mundo de la vida de los sujetos con los cuales interactúa en toda práctica profesional. En este trabajo se aborda en un primer momento el concepto de mundo de la vida; luego se indaga su relación con los sujetos sociales y con la cotidianidad de la vida social; y finalmente su relación con el Trabajo Social.","palabras_clave":"TRABAJO SOCIAL|MUNDO DE LA VIDA|SUJETOS SOCIALES|PRÁCTICA PROFESIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3281,"idioma_id":4,"titulo":"Polyurethane foams based on modified tung oil and reinforced with rice husk ash I: Synthesis and physical chemical characterization","resumen":"A chemically modified tung oil was used as the main polyol component in the formulationof viscoelastic (low resilience) polyurethane foams. Rice Husk Ash (RHA), a residue fromthe rice process industry, was chosen to be incorporated as rigid filler in these materialsbecause of its high silica content.Water was used as blowing agent in order to increase thegreen nature of the reinforced foams. Physico-chemical and thermal properties of the neatand reinforced foams were measured and analyzed. RHA addition leads to noticeablechanges in several properties, mainly thermal conductivity, density and foam morphology,even at the low filler content used in this work. Although the thermal stability was almostunaffected by ash content, a stabilizing effect of the inorganic filler was identified, sincethe residual char was higher than predicted from theoretical calculations.","palabras_clave":"TUNG OIL|NATURAL POLIOL|RICE HUSK ASH|FILLED POLYURETHANE FOAMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3282,"idioma_id":4,"titulo":"Aggregate Structural Transitions Noticed for the Didodecyldimetil-ammonium Bromide-Sodium Dehydrocholate Catanionic Mixed System at Low Concentration.","resumen":"The phase behavior for the very diluted DDAB-NaDHC-water system was investigated at 25&#9702;C. Fluorescence, conductivity, and surface  tension experiments were carried out to determine the cac values. Comparison between experimental and calculated values shows the  suitability of the regular theory to predict the critical aggregation concentrations for the tested systems. The interaction between both  amphiphiles inside aggregates is not ideal, showing a large synergism. Such fact is reflected by the activity of coefficients values. The  aggregates are mainly comprised by DDAB (18?30 % mol NaDHC) regardless of the NaDHC solution molar fraction; these molecules  act as a good solvent of bile salt type molecule. Nevertheless, the gradual inclusion of NaDHC molecules inside DDAB bilayers leads to  structural transformations. These facts are supposed to be due the combination of two effects: (i) the reduction of head groups hydration and  (ii) the increment of chain repulsion","palabras_clave":"Didodecyldimetil-ammonium Bromide|Sodium Dehydrocholate Catanionic |Mixed System at Low Concentration."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3283,"idioma_id":4,"titulo":"Draft Genome Sequence of the Polyextremophilic Exiguobacterium sp. Strain S17, Isolated from Hyperarsenic Lakes in the Argentinian Puna","resumen":"Exiguobacterium sp. strain S17 is a moderately halotolerant, arsenic-resistant bacterium that was isolated from Laguna Socompa stromatolites in the Argentinian Puna. The draft genome sequence suggests potent enzyme candidates that are essential for survival under multiple environmental extreme conditions, such as high levels of UV radiation, elevated salinity, and the presence of critical arsenic concentrations.","palabras_clave":"Bioinformatica|Microbiologia|Extremofilos|Arsenico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3284,"idioma_id":4,"titulo":"Ab Initio Studies of CO Adsorption on PdGa(110)","resumen":"CO adsorption is analyzed using density functional theory (DFT) calculations. Changes in the electronic structure of PdGa(110) surface and CO bond after adsorption are addressed. CO is located only on Pd atop geometry with a tilted configuration (9.13° from the perpendicular to the surface) and no interaction with Ga is detected. The Pd-Pd bond strength decreases 54.2 % as the new Pd-CO bond is formed. The C-O bond length change less than 1%, compared to the gas phase value, while its bond overlap population decrease 46.2%. The effect of CO is limited to its first Pd neighbor. Analysis of orbital interaction reveals the Pd-CO bond mainly involves s-s and s-p orbitals with less participation of Pd 4d orbitals. The computed CO vibration frequencies after adsorption shows a red shift from vacuum to ward 2013.85 cm-1, which agrees with previous experimental data on PdGa intermetallic.","palabras_clave":"PDGA |DFT|INTERMETALLIC COMPOUNDS|CO ADSORPTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3285,"idioma_id":4,"titulo":"Effect of chitosan on distearoylphosphatidylglycerol (DSPG) films at air\/water and liquid\/liquid interfaces","resumen":"The effect of chitosan on distearoylphosphatidylglycerol (DSPG) films was analyzed by cyclic voltammetry,surface pressure-area and surface potential-area isotherm and Brewster Angle Microscopy.Experiments of cyclic voltammetry at a liquid\/liquid interface demonstrated a blocking effect of DSPGto tetraethylammonium (TEA+) cation transfer from the aqueous to the organic phase. This effect wasreversed by the presence of chitosan, which modifies the film structure. Special emphasis was placed onthe nature of the supporting aqueous electrolyte (LiCl or CaCl2). In the presence of LiCl the permeabilityof the film increases when chitosan is present in the aqueous phase, minimizing the blocking effect of thefilm on TEA+ transfer probably due to the presence of bare zones at the interface. Oppositely, in presenceof Ca2+, the enhancement of permeability was not observed, probably due to the impediment of chitosanto penetrate into the very tightly compacted film of DSPG. Electrochemical experiments were completedwith viscosity measurements to explain the variation of diffusion coefficients for TEA+. Isotherms ofcompression for DSPG monolayers modified with chitosan, demonstrate that this polymer produces anexpansion of the DSPG film and modifies the compression factor, for both electrolytes studied. Images ofBrewster angle microscopy evidence an increase in the optical thickness of the DSPG films in presenceof chitosan indicating that the polymer interacts with DSPG molecules at low and high molecular areas.","palabras_clave":"CHITOSAN|PHOSPHOLIPIDS MONOLAYERS|LIQUID\/LIQUID INTERFACES|AIR\/WATER INTERFACES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3287,"idioma_id":4,"titulo":"Trypanocidal activity of thioamide-substituted imidazoisoquinolinones: Electrochemical properties and biological effects","resumen":"Three thioamide-substituted imidazoquinolinone, which possess a   heterocyclic center similar to tryptanthrin and are named C1,  C2, and   C3, were studied regarding (a) their in vitro anti-Trypanosoma cruzi   activity, (b) their cytotoxicity and electrochemical  behaviour, and (c)   their effect on cell viability, redox state, and mitochondrial   function.Theassayed compounds showed a significant  activity against the   proliferative forms, but only C1 showed activity on the trypomastigote   form(for C1, IC50 epi = 1.49 uM; IC50 amas =  1.74 uM; and IC50 try =   34.89 uM). The presence of an antioxidant compound such as ascorbic acid   or dithiotreitol induced a  threefold increase in the antiparasitic   activity, whereas glutathione had a dual effect depending on its   concentration. Our results  indicate that these compounds, which   exhibited low toxicity to the host cells, can be reduced inside the   parasite bymeans of the pool  of lowmolecular weight thiols, causing   oxidative stress and parasite death by apoptosis.The antiparasitic   activity of the compounds  studied could be explained by a loss of the   capacity of the antioxidant defense system of the parasite to keep its   intracellular redox  state. C1 could be considered a good candidate for   in vivo evaluation.","palabras_clave":"TRYPANOCIDAL ACTIVITY|IMIDAZOQUINOLINONE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3288,"idioma_id":5,"titulo":"Efectos reproductivos del antagonista  de GnRH, Azaline B, en el macho canino","resumen":"El azaline B es un antagonista de las hormonas liberadores de gonadotrofinas (GnRH), que se une a los receptores gonadotropos específicos de membrana y compiten con las moléculas endógenas de GnRH por la ocupación de éstos. Este estudio tiene como objetivo describir los cambios espermáticos de semen de eyaculado y de epidídimo, como así también los cambios histopatológicos de testículo en caninos domésticos tratados con el antagonista, azaline B. Se estudiaron 7 perros mestizos, clínicamente sanos, de entre 20 y 25 kg de peso y 2 a 4 años de edad. El estudio incluyó un período de pre-tratamiento y pos-tratamiento de 7 y 60 días, respectivamente. En el día 0 del protocolo se le administró al grupo tratado (AZA; n=6), 375 &#956;g\/kg de Azaline B por vía subcutánea (SC) y al grupo control (CTL; n=1), un diluyente a igual volumen vía SC. Se realizaron cada 15 días evaluaciones de consistencia testicular, libido, determinaciones de testosterona sérica, eyaculado, examen microscópico de testículos y espermatozoides epididimales. En el grupo AZA las variables cuantitativas se analizaron mediante un test de ANOVA de mediciones repetidas. Las variables estudiadas en semen de eyaculado y de epidídimo se compararon con el coeficiente de correlación de Pearson (r). En los perros medicados la libido no fue afectada por el tratamiento. La consistencia testicular, los niveles séricos de testosterona y el volumen de la segunda fracción del eyaculado variaron a lo largo del experimento con resultados no significativos. En las muestras de semen de eyaculado y de epidídimo la concentración espermática, la viabilidad y las morfoanomalías se modificaron significativamente entre los días 15 y 30 de realizado el tratamiento, existiendo una alta correlación entre ambas muestras. En los preparados histológicos, la meiosis en el epitelio seminífero descendió un 42%, los días 15 y 30, lo mismo sucedió con los porcentajes de espermatocitos primarios, espermátides y espermatozoides. En todos los casos las células retomaron la cantidad inicial al final del estudio. Se concluye que el azaline B produjo alteraciones testiculares y seminales reversibles y detectables entre los días 15 y 30 del postratamiento, lo cual permite vislumbrar a los antagonistas del GnRH como drogas futuras para ser utilizadas en contracepción y enfermedades hormonodependiente del perro.","palabras_clave":"AZALINE|CANINO|REPRODUCCION|ANTAGONISTA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3289,"idioma_id":4,"titulo":"Human Error on the Risk Reduction Actions during the NPP Maintenance (Indexed by Thomson Reuters)","resumen":"In this paper an analysis above the impact of the human error on the risk reduction actions is presented. This paper shows in a clear way the dynamic in the human error within the maintenance process like is follow in the nuclear industry around the world. The conclusions show the importance of the background knowledge of the maintenance staff and the relevance of the sequence of the risk reductions actions included in the maintenance tasks.","palabras_clave":"HUMAN ERROR|MAINTEANCE|RISK REDUCTION|NUCLEAR POWER PLANTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3290,"idioma_id":5,"titulo":"Anatomía comparada de Vertebrados. Actividades para el estudio del esqueleto. Cráneo de Anfibios, Reptiles, Aves y Mamíferos","resumen":"En las prácticas se estudia el esqueleto postcraneal y apendicular de los distintos grupo de Vertebrados. Se realizan observaciones, identificaciones de las estructuras y de las piezas esqueléticas y se analizan de una forma comparada.","palabras_clave":"VÉRTEBRAS|ALETAS|CINTURAS|QUIRIDIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3292,"idioma_id":4,"titulo":"Modulation Of Pancreatic Tumor Potential By Overexpression Protein Kinase C Beta1 (PKCb1)","resumen":"Objective: to investigate whether the overexpression of PKCb1 is able to modulate the malignant phenotype displayed by the human ductal pancreatic carcinoma cell line PANC1.  Methods: PKCb1 overexpression was achieved using a stable transfection approach. PANC1-PKCb1 and control cells were analyzed both in vitro and in vivo.  Results: PANC1-PKCb1 cells displayed a lower growth capacity associated with the downregulation of the MEK\/ERK pathway and cyclin expression. Furthermore, PKCb1 overexpression was associated with an enhancement of cell adhesion to fibronectin and with reduced migratory and invasive phenotypes. In agreement with these results, PANC1-PKCb1 cells showed an impaired ability to secrete proteolytic enzymes. We also found that PKCb1 overexpressing cells were more resistant to cell death induced by serum deprivation, an event associated with G0\/G1 arrest and the modulation of PI3K\/Akt and NF-kB pathways. Most notably, the overexpression of PKCb1 completely abolished the ability of PANC1 cells to induce tumors in nude mice.  Conclusions: our results established an important role for PKCb1 in PANC1 cells suggesting it would act as a suppressor of tumorigenic behavior in pancreatic cancer.","palabras_clave":"Human pancreatic adenocarcinoma,|PKC\u0002\u0003|tumor growth inhibition."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3293,"idioma_id":5,"titulo":"ESPACIAMIENTOS DENDRÍTICOS SECUNDARIOS VERSUS RESISTENCIA A LA CORROSIÓN EN ACEROS 316","resumen":"Las aleaciones presentan una macroestructura característica que se forma a partir del proceso de solidificación desolidificación direccional, consistente en tres tipos principales de estructuras de granos: columnar, equiaxial, y unazona de transición de columnar a equiaxial (TCE). También se forma una microestructura dendrítica como forma decristalización. Estas estructuras hacen que las aleaciones tipo acero 316 presenten modificaciones en lascaracterísticas mecánicas y en la resistencia a la corrosión. Con el objeto de estudiar la resistencia a la corrosión enlos aceros en el presente trabajo se utilizaron probetas de diferente composición, a las cuales se les realizaron ensayosde polarización potenciodinámica cíclica para cada tipo de estructura (columnar, equiaxial y con TCE). Acontinuación se reveló la microestructura de cada probeta y se midieron los espaciamientos dendríticos secundarios.La mayor resistencia a la corrosión fue observada en las estructuras columnar y con TCE, mientras que la estructuraequiaxial fue la más susceptible en la mayoría de los casos. Se encontraron mayores valores de espaciamientosdendríticos en la zona equiaxial de las probetas.","palabras_clave":"Corrosión|Materiales|Acero|Estructura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3294,"idioma_id":5,"titulo":"Décimo Septimo Informe Semestral sobre Política Exterior del gobierno argentino (septiembre de 2011 a marzo de 2012)","resumen":"El informe evalúa las principales acciones de la política exterior entre septiembre de 2012 a marzo de 2013.","palabras_clave":"Relaciones |Internacionales|Política|Exterior"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3295,"idioma_id":4,"titulo":"A search for prompt lepton-jets in $pp$ collisions at $\\sqrt{s}=7$ TeV with the ATLAS detector","resumen":"We present a search for a light (mass &lt;2GeV) boson predicted by Hidden Valley supersymmetric models that decays into a final state consisting of collimated muons or electrons, denoted ''lepton-jets''. The analysis uses 5fb^-^1 of s=7TeV proton-proton collision data recorded by the ATLAS detector at the Large Hadron Collider to search for the following signatures: single lepton-jets with at least four muons pairs of lepton-jets, each with two or more muons and pairs of lepton-jets with two or more electrons. This study finds no statistically significant deviation from the Standard Model prediction and places 95% confidence-level exclusion limits on the production cross section times branching ratio of light bosons for several parameter sets of a Hidden Valley model.We present a search for a light (mass &lt; 2 GeV) boson predicted by Hidden Valley supersymmetric models that decays into a final state consisting of collimated muons or electrons, denoted 'lepton-jets'. The analysis uses 5fb-1 of sqrt(s) = 7 TeV proton--proton collision data recorded by the ATLAS detector at the Large Hadron Collider to search for the following signatures: single lepton-jets with at least four muons; pairs of lepton-jets, each with two or more muons; and pairs of lepton-jets with two or more electrons. This study finds no statistically significant deviation from the Standard Model prediction and places 95% confidence-level exclusion limits on the production cross section times branching ratio of light bosons for several parameter sets of a Hidden Valley model.","palabras_clave":"ATLAS|high|energy|physics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3360,"idioma_id":5,"titulo":"Vientos para el cambio. Territorios, energía eólica y cooperativas de electricidad en el sur bonaerense","resumen":"La disponibilidad de electricidad es un factor estratégico para el desarrollo socioproductivo y las redes de infraestructura energética constituyen un elemento vertebrador de la integración del sistema económico y territorial. La existencia de grandes distancias entre los centros de producción y los de transformación y consumo, junto con la imposibilidad de almacenar la electricidad producida, hacen necesario el desarrollo de una red técnica que asegure la circulación, vinculando los sistemas de transporte con los puntos físicos donde los usuarios requieren la electricidad. Este trabajo presenta a las cooperativas de distribución de electricidad de la provincia de Buenos Aires como eslabones centrales del proceso de distribución a escala local y regional. Asimismo, en un contexto de estímulo a la producción descentralizada, se exploran los nuevos escenarios y desafíos que plantean a las cooperativas, las posibilidades abiertas por los proyectos de generación a partir de fuentes eólicas en el sur provincial.","palabras_clave":"TERRITORIO|DISTRIBUCIÓN DE ELECTRICIDAD|COOPERATIVAS|ENERGÍA EOLICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3362,"idioma_id":4,"titulo":"Taphonomic Marks on Pig Tissue Due to Cadaveric Coleoptera Activity Under Controlled Conditions","resumen":"The aim of this work was to study taphonomic marks that cadaveric coleopteran can produce under controlled conditions. To evaluate this, pig trotters were initially exposed to adults of Dermestes maculatus De Geer, 1774 (1) at 21±5°C and 12:12 hour day\/night cycle. Observations were performed and photographs were taken every 4 &#822; 5 days for nine months. When feeding and reproducing, Dermestes maculatus in both adult and larvae stages produced different types of marks such as holes, striations, scratches and pits in several kinds of tissue such as integumental, connective and muscular, in both their fresh and dried statuses. Bite marks were also evidenced. In this study, results provide not only taphonomic but also biological and forensic information. This is the first time that this kind of experiment has been performed.","palabras_clave":"forensic science|forensic taphomomy|Dermestes maculatus|carrion fauna"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3365,"idioma_id":4,"titulo":"Gemini 3D spectroscopy of the BCD galaxy Haro 15: Evidence for gas outflow and expanding supershell","resumen":"We present Integral Field Unit (IFU) - GMOS data of the Wolf-Rayet galaxy Haro 15 obtained at the Gemini South telescope. The good spatial and spectral resolution of these GMOS-IFU observations, covering 3700-10000 &#778;A allowed us to map with excellent signal-to-noise ratio the velocity field and flux distribution of H&#945;, H&#946;, [Oiii] &#955; 5007  &#778;A emission lines. Careful comparison of continuum and emission line flux contributions allowed us to identify nine and four sources within Complex A and B respectively. A global rotation map was derived for the whole galaxy, which reveals signature of a merging process although it can be fitted with a relatively simple nucleus & disk mass distribution ruling out the possibility of a double nucleus. Detailed kinematical analysis within the starburst complexes shows the presence of strong outflows which can reach kiloparsec distances. The most luminous star forming region, the B1 knot within Complex B, lies close to the edge of a superbubble which center matches the position of the continuum source B4. The ages of the stellar populations from each source, together with the size and evolutionary timescale of the supershell, fit a triggered star-formation scenario within Complex B.","palabras_clave":"ISM: kinematics and dynamics|Galaxies: starburst|Galaxies: individual (Haro 15)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3366,"idioma_id":5,"titulo":"Reseña del libro 'Los montoneros del barrio', de Javier Salcedo","resumen":"El trabajo de Javier Salcedo revisa el caso de los Montoneros de la localidad de Moreno, una experiencia escasamente conocida en el campo de los estudios sobre la izquierda peronista. El libro comparte una filiación común con las investigaciones de Lucas Lanusse sobre los orígenes de Montoneros, de Cristina Viano sobre la formación de Montoneros en Rosario, y de Horacio Robles sobre la Juventud Peronista de La Plata, entre otros aportes. El denominador en común de estas pesquisas es que ponen en crisis los supuestos más débiles o menos explorados de la obra de Gillespie, como los antecedentes de los primeros dirigentes montoneros en agrupaciones nacionalistas de derecha, los análisis de casos regionales, o bien la dinámica y composición de los frentes de masas de la organización guerrillera. Salcedo estudia la trayectoria de las organizaciones locales que confluirían en Montoneros entre 1968 y 1974, fecha que marca la ruptura entre la conducción montonera y los militantes de Moreno, que se reagruparían en la Juventud Peronista Lealtad. El problema que orienta la investigación consiste en determinar por qué varios jóvenes de extracción obrera, y antiguos protagonistas de la resistencia peronista que militaban en Moreno se sumaron a Montoneros, articulando el trabajo de base con la práctica armada, y vinculándose a activistas provenientes de las capas medias de la sociedad argentina.","palabras_clave":"Montoneros|Lucha armada|Violencia política|inserción de masas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3367,"idioma_id":4,"titulo":"Susceptibility of Nezara viridula (L.) (Hemiptera: Pentatomidae) Egg Masses of Different Sizes to Parasitism by Trissolcus basalis (Woll.) (Hymenoptera: Platygastridae) in the Field. Biological Control.","resumen":"Egg masses of Nezara viridula (L.) are commonly parasitized by Trissolcus  basalis (Woll.), and we investigated the role of size of egg masses on  parasitization by T. basalis . Sentinel egg masses were exposed to parasitism  in the field for 6?7 days, when they were collected for evaluation of  parasitoid emergence. We recorded the number of eggs per egg mass,  the number of emerged hosts, and the number of empty and parasitized  eggs. We calculated the proportion of attacked host egg masses (DE), the  proportion of parasitized eggs per attacked egg mass (PE), and total parasitism  (PI). The total number of egg masses exposed to parasitism was 330.  The minimum, mean, and maximum egg mass sizes were 25, 75.2, and 111,  respectively. DE and PE varied widely between different fields, and they  were independent of egg mass size. In 14.2% of all parasitized egg masses,  we found simultaneous emergence of T. basalis and N. viridula independently  of host egg mass size. PE exhibited low variability compared with PI  and DE, which were linearly related. PI and DE values from other field  studies are consistent with the linear relationship, suggesting that PI is  mostly related to the proportion of the DE. This also suggests that total  parasitism is independent of egg mass size, of possible differences in plant  species, and T. basalis density and strains.","palabras_clave":"stink bugs|egg mass size|egg parasitoids|field parasitism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3368,"idioma_id":5,"titulo":"Perfil de apropiación de energías renovables a partir de una evaluación de seguimiento","resumen":"    Una  evaluación de proyecto debe ser programada como un proceso transversal e  involucrar una valoración ex ante, de seguimiento o proceso, de resultados e  impacto. El presente trabajo se refiere a aspectos principales de una  evaluación de seguimiento de la planificación de un proyecto de transferencia y  apropiación de cocinas solares, en un contexto peri urbano de pobreza. Se  identifican los errores incurridos o aspectos susceptibles de optimizar, con  énfasis en autocrítica al equipo dinamizador, a fin de lograr una  retroalimentación - reconducción y configurar algunos aportes a una Metodología  de la Transferencia de Energías Alternativas.&nbsp;  Los resultados se presentan en&nbsp; un  Diagrama Circular dando cuenta de&nbsp; una  visión holística expresada en un Perfil de Apropiación. El mismo incluye  demarcaciones para visualizar cómo se presenta cada variable en el contexto.  Atribuye grados de significación como problemas, expresados mediante categorías  surgidas de ponderaciones diferenciales. El seguimiento analiza básicamente la  experiencia en el asentamiento Los Bajos, distrito San Isidro- Valle Viejo,  Catamarca, Argentina, pero se sustenta además en otros desarrollos efectuados  por el equipo.El Perfil de apropiación permitió determinar los principales  aspectos a ser trabajados en nuevas intervenciones, respecto a lo actuado por  el equipo dinamizador.   ","palabras_clave":"EVALUACION|AUTOCRITICA|PERFIL DE APROPIACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3370,"idioma_id":4,"titulo":"Assessing the conservation of a vulnerable felid species: threats from current human land use and future climate change on kodkod (Leopardus guigna)","resumen":"Climate change and habitat fragmentation are considered key pressures on biodiversity. Particularly vulnerable are mammal carnivores with small geographic distributions. Kodkod (Leopardus guigna), is a small felid endemic to the temperate forests of southern Chile and Argentina and has the smallest geographic range of any of the New World felids. Although field studies on this species have been increasing in the past decades, its distribution is not yet fully understood. Protected areas exist where the species occurs in both countries, though it is not known how well they protect the kodkod at present or under a climate change scenario. In this study, we use species distribution models and spatial analyses to assess the distribution of kodkod, including changes in human land use and future climate change. We also assess the species? current representation in protected areas and in light of a climate change scenario. Our study shows that kodkod has already suffered range loss due to human land use (5.5%), particularly in core areas with intermediate habitat suitability. Climate change, together with human land use, will affect 40% of kodkod`s present potential distribution by the year 2050. Currently, 12.5% of the species` potential distribution is represented in protected areas and, it will be represented by 14% in the future; a larger relative proportion will be protected in Argentina than in Chile. But, the species is more prone to get extinct in Argentina and Chilean Matorral.","palabras_clave":"MAXENT|SPECIES DISTRIBUTION MODELLING|WORLDCLIM|PROTECTED AREAS|TEMPERATE FORESTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3371,"idioma_id":5,"titulo":"De la 'desmalvinizaciòn' a la regionalizaciòn del reclamo argentino por la soberanía sobre las Islas Malvinas","resumen":"Este artículo analiza la trayectoria de la política argentina respecto del reclamopor la soberanía sobre las Islas Malvinas desde las décadas de la ?desmalvinización? hasta el proceso de ?regionalización? del conflicto enlos años recientes. El trabajo analiza la pérdida de centralidad de la causade Malvinas operada con el fin de la guerra del ?82 y bajo la errática políticade seducción del menemismo, y su recuperación en el ciclo kirchnerista.Por último, se destacan los éxitos de la estrategia de internacionalizacióndel conflicto en el ámbito regional sudamericano.","palabras_clave":"soberanía|regionalización|malvinas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3372,"idioma_id":4,"titulo":"Occurrence of South American fur seals Arctocephalus australis (Zimmermann, 1783) in San Matías Gulf, Patagonia, Argentina","resumen":"The South American fur seal, Arctocephalus australis (SAFS) population suffered a drastic reduction due to commercial exploitation during the eighteenth and nineteenth centuries. In the last decades a population recovery was detected on the Atlantic region. However, in this region, many aspects of the ecology of the SAFS, such as the post-reproductive dispersal of individuals, the location of feeding areas, and the migration routes between colonies on the boundaries of its distribution, are still unknown. Here, we report for the first time the occupation of San Matías Gulf (SMG, Northern Patagonia, Argentina) by this species. We found that more than 1,600 SAFS used SMG between May and October (post-reproductive season) and detected a non- reproductive colony on Lobos Islet (41°24´S, 65°03´W). The presence of SAFS in SMG is recent and would be associated with an increase of the population. The importance of SMG in the ecology of SAFS seems to lie on three factors: the strategic location in the geographic context of its potential migration routes, the physical environment suitable for coastal settlements, and the availability of food resources.","palabras_clave":"South American fur seals|Arctocephalus australis|San Matías Gulf|Islote Lobos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3373,"idioma_id":4,"titulo":"New handbook for standardised measurement of plant functional traits worldwide","resumen":"Plant functional traits are the features (morphological, physiological, phenological) that represent ecological strategies and determine how plants respond to environmental factors, affect other trophic levels and influence ecosystem properties. Variation in plant functional traits, and trait syndromes, has proven useful for tackling many important ecological questions at a range of scales, giving rise to a demand for standardised ways to measure ecologically meaningful plant traits. This line of research has been among the most fruitful avenues for understanding ecological and evolutionary patterns and processes. It also has the potential both to build a predictive set of local, regional and global relationships between plants and environment and to quantify a wide range of natural and human-driven processes, including changes in biodiversity, the impacts of species invasions, alterations in biogeochemical processes and vegetation\u2013atmosphere interactions. The importance of these topics dictates the urgent need for more and better data, and increases the value of standardised protocols for quantifying trait variation of different species, in particular for traits with power to predict plant- and ecosystem-level processes, and for traits that can be measured relatively easily. Updated and expanded from the widely used previous version, this handbook retains the focus on clearly presented, widely applicable, step-by-step recipes, with a minimum of text on theory, and not only includes updated methods for the traits previously covered, but also introduces many new protocols for further traits. This new handbook has a better balance between whole-plant traits, leaf traits, root and stem traits and regenerative traits, and puts particular emphasis on traits important for predicting species\u2019 effects on key ecosystem properties. We hope this new handbook becomes a standard companion in local and global efforts to learn about the responses and impacts of different plant species with respect to environmental changes in the present, past and future.","palabras_clave":"biodiversity|ecophysiology |ecosystem dynamics|ecosystem functions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3374,"idioma_id":4,"titulo":"Vibrational and Structural Behavior of l-Cysteine Ethyl Ester Hydrochloride in the Solid State and in Aqueous Solution","resumen":"The aim of this work is to evaluate the vibrational and structural13 properties of L-cysteine ethyl ester hydrochloride (CE), and its electronic behaviorproperties of L-cysteine ethyl ester hydrochloride (CE), and its electronic behavior 14 mainly in relation to the action of the thiol and amine groups at different degrees ofmainly in relation to the action of the thiol and amine groups at different degrees of 15 solvation. The crystal structure of CE was determined at room temperature by X-solvation. The crystal structure of CE was determined at room temperature by X- 16 ray diffraction methods. Infrared and Raman spectra were collected to compare theray diffraction methods. Infrared and Raman spectra were collected to compare the 17 behavior of different functional groups in the molecule, both in the solid phase andbehavior of different functional groups in the molecule, both in the solid phase and 18 in aqueous solution. Its UV and circular dichroism spectra were also measured inin aqueous solution. Its UV and circular dichroism spectra were also measured in 19 aqueous solution. The influence of an aqueous environment on the CE spectra wasaqueous solution. The influence of an aqueous environment on the CE spectra was 20 simulated by means of implicit (polarizable continuum model) and explicitsimulated by means of implicit (polarizable continuum model) and explicit 21 (molecular dynamics, solute&#8722;solvent clusters) methods. Calculations in explicit and(molecular dynamics, solute&#8722;solvent clusters) methods. Calculations in explicit and 22 continuous solvent are of interest to explain the behavior of bioavailable sites in thiscontinuous solvent are of interest to explain the behavior of bioavailable sites in this 23 medium. The study was completed by natural bond orbital analysis to determinemedium. The study was completed by natural bond orbital analysis to determine 24 the presence of hyperconjugative interactions.the presence of hyperconjugative interactions.","palabras_clave":"L-cysteine ethyl ester hydrochloride|polarizable continuum model|natural bond orbital analysis|structural"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3376,"idioma_id":4,"titulo":"Correlations between physical and chemical defences in plants: Trade-offs, syndromes, or just many different ways to skin a herbivorous cat?","resumen":"Most plant species have a range of traits that deter herbivores. However, understanding of how different defences are related to one another is surprisingly weak. Many authors argue that defence traits trade off against one another, while others argue that they form coordinated defence syndromes. We collected a dataset of unprecedented taxonomic and geographic scope (261 species spanning 80 families, from 75 sites across the globe) to investigate relationships among four chemical and six physical defences. Five of the 45 pairwise correlations between defence traits were significant and three of these were tradeoffs. The relationship between species? overall chemical and physical defence levels was marginally nonsignificant (P = 0.08), and remained non significant after accounting for phylogeny, growth form and abundance. Neither categorical principal component analysis (PCA) nor hierarchical cluster analysis supported the idea that species displayed defence syndromes. Our results do not support arguments for tradeoffs or for coordinated defence syndromes. Rather, plants display a range of combinations of defence traits. We suggest this lack of consistent defence syndromes may be adaptive, resulting from selective pressure to deploy a different combination of defences to coexisting species.","palabras_clave":"cyanogenesis|nectaries|hair|leaf toughness"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3377,"idioma_id":4,"titulo":"Shedding of TNFR1 induced by protein A decreases TNF-&#945; availability and inflammation during systemic Staphylococcus aureus infection","resumen":"Staphylococcus aureus infections are an important  concern in public health due to their increasing incidence and high rates of  mortality. The S. aureus success as a pathogen is highly related to its  enormous capacity to evade the host immune response. The critical role of  TNF-alpha in the initial host defense against staphylococcal systemic infection  has been demonstrated in experimental models and may partially explain the lack  of significant benefits observed in clinical trials attempting to neutralize  this cytokine in septic patients. S. aureus protein A plays a key role in  regulating inflammation through its ability to bind and signal through the TNF-alpha  receptor (TNFR1). In  this study, we demonstrate that S. aureus, via protein A mediated signaling, induces  early shedding of soluble TNFR1 which precedes the secretion of TNF-alpha in  vitro and in vivo. The results obtained using a protein A deficient mutant and  tnfr1-\/- mice strongly suggest that the increased levels of soluble TNFR1  present during S. aureus experimental infection may neutralize circulating  TNF-alpha and impair the host inflammatory response. Early shedding of soluble  TNFR1 induced by protein A may constitute a novel mechanism by which S. aureus  subverts the host immune response.","palabras_clave":"TNFR1|PROTEIN A|SHEDDING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3378,"idioma_id":4,"titulo":"Endophytic bacteria from Ilex paraguariensis shoot cultures: their localisation, characterisation, and response to isothiazolone biocides","resumen":"Nodal segment explants of Ilex paraguariensis,collected from greenhouse-grown plants, were found tocontain endophytic bacteria. After culturing in bioreactors,16 rRNA gene analyses and analytical profile index biochemicaltests were used to identify these bacteria asStenotrophomonas malthophilia. The presence of bacterialcells in the intercellular spaces of stem cortical parenchymawas detected in histological sections by scanning electronmicroscopy. A range of commercial isothiazolone biocideswere tested for their ability to repress the growth of Gramnegativebacteria grown in liquid media during themicropropagation phase. The addition of 0.75 ml l&#8722;1Delcide? TG (5-chloro-2-methyl-4-isothiazolin-3-one + 2-methyl-4-isothiazolin-3-one, 1.05% and 0.45%, respectively)to the culture media resulted in 100% visibly clean cultures,with no suppression of shoot growth.","palabras_clave":"BIOREACTORS|CONTAMINATION|16S rDNA ANALYSIS|WOODY PLANT SPECIES|MICROPROPAGATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3379,"idioma_id":4,"titulo":"Larvicidal activity of crude extracts from Larrea cuneifolia (Zygophyllaceae) and its metabolite the nordihydroguaiaretic acid, against the vector Culex quinquefasciatus (Diptera: Culicidae)","resumen":"Introduction: The aim of the present study was to analyze the larvicidal activity of different crude extracts of Larrea cuneifoliaand its most abundant lignan, nordihydroguaiaretic acid (NDGA), against Culex quinquefasciatus. Methods: Chloroform,methanol, and aqueous extracts from L. cuneifolia and NDGA were tested against larvae of Cx. quinquefasciatus under laboratoryconditions. Results: The chloroform extract showed the highest larvicidal effect, with an estimated LC50 of 0.062 mg\/ml. NDGAalso demonstrated significant larvicidal activity with an estimated LC50 of 0.092 mg\/ml. Conclusions: These results indicate thatthe chloroform extract of L. cuneifolia and NDGA are promising insecticides of botanical origin that could be useful for controllingCx. quinquefasciatus.","palabras_clave":"Larrea cuneifolia|Culex quinquefasciatus|larvicidal"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3380,"idioma_id":4,"titulo":"On-line enantioseparation of chlorpheniramine using -cyclodextrin and  carbon nanotubes after multivariate optimization","resumen":"Multiwalled carbon nanotubes are evaluated here as solid phase extraction (SPE)sorbent aiming to (±)-chlorpheniramine (CPA) enantioresolution with fluorimetric detection. b-cyclodextrin (CD) was added to the racemate and solutions with HCl and sodium dodecyl sulfate (SDS) in different proportions were assayed as eluents to achieve the separation between both enantiomers. The overall methodology involved a flow injection (FI) strategy enabling high sample throughput and low reagents consumption making it suitable for drug routine quality control. An adequate  enantioresolution (2.08) with satisfactory responses for both (R)-CPA (peak area = 285) and (S)-CPA (peak area = 380) was achieved applying the proposed FI-SPE strategy under the optimized conditions [b-CD] = 1.0 mmol L-1, [HCl] = 1.0 x 10-2 mol L-1, [SDS] = 4.0 x 10-4 mol L-1 and eluent flow rate = 8.0 rpm.","palabras_clave":"On-line enantioresolution |Carbon nanotubes-beta-cyclodextrin|Flow injection analysis|Multivariate optimization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3381,"idioma_id":5,"titulo":"Re-Conociendo un espacio. Prospecciones en la Quebrada de Miriguaca (Antofagasta de la Sierra, Catamarca)","resumen":"El propósito de este trabajo es dar a conocer los primeros resultados obtenidos en el marco del proyecto de investigación ?Uso del espacio y ocupación diferencial en la Puna Meridional: el caso quebrada río Miriguaca&nbsp; (Departamento Antofagasta de la Sierra, Catamarca)?, que tiene como objetivo general profundizar en el estudio de las estrategias de uso del espacio y manejo de recursos en un amplio lapso, que transcurre entre 5000 y 700 años AP. Específicamente, la propuesta de este proyecto pretende evaluar los alcances de diversos modelos de ocupación del espacio ya explicitados previamente por otros investigadores. Se pretende así, con el análisis de nueva evidencia arqueológica procedente de la quebrada de Miriguaca, avanzar en aspectos que conlleven a enriquecer la discusión teórica vinculada con la variabilidad inherente a los modelos de uso del espacio vigentes. En este sentido, cabe destacar que, hasta el momento, esta quebrada ha sido escasamente explorada desde el punto de vista arqueológico, de modo tal que las investigaciones se encuentran prácticamente en sus inicios.&nbsp; En esta ocasión particular, damos a conocer los resultados de las prospecciones sistemáticas (incluyendo una recolección superficial discriminada y sondeos estratigráficos exploratorios) realizadas en el curso inferior y medio del río Miriguaca, las que se concretaron durante los trabajos de campo correspondientes a las temporadas 2006 y 2007. El registro arqueológico recuperado y algunos fechados radiocarbónicos obtenidos nos permitieron establecer un ordenamiento cronológico preliminar de la mayoría de los sitios relevados durante las prospecciones. Cabe destacar que los resultados obtenidos adquieren relevancia desde el momento que se trata de una zona prácticamente inexplorada desde el punto de vista arqueológico. A continuación presentamos una breve caracterización del área de estudio, enfatizando en su aspecto ambiental y en las potencialidades que ofrece el medio natural para la ocupación humana. Se consignan además los antecedentes de investigaciones arqueológicas realizadas en el área del río Miriguaca, a los fines de bosquejar el marco de conocimiento previo con el que se contaba al momento de iniciar nuestras investigaciones.","palabras_clave":"USO DEL ESPACIO |PROSPECCIONES|QUEBRADA DE MIRIGUACA|PUNA|NOA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3382,"idioma_id":4,"titulo":"Morphogenesis at tillers level in populations of Trichloris crinita.(Abstract)","resumen":"Trichloris crinita is a summer perennial species present in the natural grassland of arid and semi-arid regions of Argentina. The study was carried on a parcel located in the experimental field of Agronomy of UNSL (San Luis). In spring and summer were recorded and evaluated morphogenetic and structural variables in tillers at three populations: San Luis, Catamarca and Mendoza. In spring was made a clean cut (CL: 15\/10\/12) and identified 25 tillers. In summer, there were marked 14 tillers after a defoliation performed when most of these reached the first leaf senescence. In each tiller were measured every 7 days to determine the following morphogenetic variables: leaf appearance rate (TAH), mean leaf lifespan (VMF) and elongation rate (TEF); and structural variables: number of green leaves (NHV), first choice foliar leaf length (LF1H), distal length of tiller (LD) and number of tillers children. For each growing season, we applied a multivariate analysis (Ward), considering the variables of the 3 populations. In both seasons were discriminated two clusters, one corresponded to the population Catamarca, and another to San Luis and Mendoza. Catamarca showed an increased foliar refill and greater poise, while the remaining populations had a slower foliar refill. Mendoza had a smaller poise than San Luis. The Catamarca population could be adapted to grazing systems with less break time in the study area.","palabras_clave":"mean life lifespain"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3383,"idioma_id":4,"titulo":"Characterization and seasonal changes in LHbeta; and FSHbeta; mRNA of Rhinella arenarum (Amphibia, Anura)","resumen":"In anurans, two types of gonadotropins were described in several species of Ranidae and Pipidae families but only in one of the Bufonidae family. Rhinella arenarum is a bufonid that have the lowest concentration of plasma androgens during the breeding. The objective of this paper was to characterize the cDNA sequence of &#946; subunit of LH and FSH from toad pituitary and study seasonal variation in gonadotropins mRNA using quantitative real-time RT-PCR. The LH&#946; cDNA is a 636 bp sequence containing an open reading frame (ORF), 45 bp of 5´-untranslated region (UTR) and 174 bp of 3´-UTR. The ORF encodes for a signal peptide of 26 amino acids and a mature protein of 113 amino acids with one N-glycosylation site at the 34th position. The FSH&#946; cDNA sequence is a 535 bp fragment containing an ORF, 8 bp of 5´-UTR and 152 bp of 3´-UTR. The ORF encodes for a signal peptide of 20 amino acids and a mature protein of 104 amino acids with two N-glycosylation sites at 25th and 42nd positions. Multiple alignments of aminoacid deduced sequences of LH&#946; and FSH&#946; (teleosts, amphibians, birds, mammals) showed that all the tetrapods studied conserve 12 cysteins and one (LH) or two (FSH) N-Glycosylation sites. LH&#946; is closer to teleosts than to mammals and birds while FSH&#946; is closer to mammals. The analysis of seasonal changes in LH&#946; and FSH&#946; mRNA indicates that transcript levels have seasonal variations and that the profile of androgens is opposite to that of the gonadotropins mRNA.  Copyright © 2013 Elsevier In","palabras_clave":"PITUITARY|GONADOTROPINS|TOAD|SEASONAL VARIATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3446,"idioma_id":4,"titulo":"Conceptual Model for the Integration of PRONTO with ISO Standard 15926","resumen":"In this paper a conceptual model based on the ISO 15926 standard is proposed in order to extend and make explicit the representation of product variants given by PRONTO. This extension will allow introducing the product model in a collaborative environment where information systems are able to exchange information with other products systems. Interoperability of systems tends to increase the consistency of models, due to they may have several interpretations. Therefore, the proposal defines new elements and adapts these to the data model of the standard, for a complete concept mapping of PRONTO. Furthermore, changes in the product information are represented in time, through 4D approach.","palabras_clave":"Interoperability|ISO 15926|Product Family|PRONTO|4D approach|Semantic Web"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3451,"idioma_id":4,"titulo":"Pesticide potential dermal exposure during the manipulation of concentrated mixtures at small horticultural and floricultural production units in Argentina: The formulation effect","resumen":"Potential dermal exposure measurements of horticultural and floricultural field operators that handled concentrated  pesticides showed a correlation with the types of formulations used (liquid or solid) during the mix and  load stage. For liquid formulations, hand exposure was 22?62 times greater than that for solid ones. The dermal  exposure mechanism was studied for this formulation under laboratory conditions, finding that the rupture of  the aluminum seal of the pesticide container and the color of the liquid formulation are important factors. Additionally,  significant external surface contamination of pesticide containers collected at horticultural farms was  found. This could partially account for the differences between the exposure levels of field and laboratory experiments  for liquid formulations.","palabras_clave":"Potential Manual Exposure|Margin of Safety|Horticulture|Floriculture"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3452,"idioma_id":4,"titulo":"Molecular Solvent Effects on the Acidity Constant of Protic Ionic Liquids","resumen":"In this work how the microscopic properties of a molecular solvent affect the chemical environment of the protic ionic liquids (PILs) was analyzed. Using Reichardt?s dye as indicator of acidity, new acidity constant values for eight PILs (pKa PILs) were determined by spectrophotometric titration. Modifying the character hydrogen bonding donor of the molecular solvent it is possible to handle the PIL acid strength. Thus, we can turn basic PILs into acidic ones thereby the molecular solvent could be used as ?additive? for PILs, which allowed us to tune PILs design.","palabras_clave":"PROTIC IONIC LIQUIDS|REICHARD'S DYES|PKA|H BONDING INTERACT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3453,"idioma_id":4,"titulo":"Some advances on Lovász and Schrijver semidefinite programming relaxations of the fractional stable set polytope","resumen":"We study Lovász and Schrijver´s hieararchy of relaxations based on positive semideniteness constraints derived from the fractional stable set polytope. We show that there are graphs G for which a single application of the underlying operator, N+, to the fractional stable set polytope gives a nonpolyhedral convex relaxation of the stable set polytope. We also show that none of the current best combinatorial characterizations of these relaxations obtained by a single application of the N+ operator is exact.","palabras_clave":"STABLE SET PROBLEM|LIFT-AND-PROJECT|SEMIDEFINITE PROGRAMMING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3454,"idioma_id":5,"titulo":"La libertad en el rock argentino: El discurso y la reconstrucción democrática","resumen":"El proyecto de beca y asimismo de Tesis Doctoral propone analizar las concepciones y las denominaciones de libertad que atraviesan las construcciones discursivas de las líricas del rock argentino del período 1982-1989, desde la finalización de la guerra de Malvinas hasta la renuncia del ex presidente Raúl Alfonsín. La puesta en acción del análisis discursivo lingüístico permite prosperar, reflexiva y sistemáticamente, una producción de sentido en torno a los actos de comunicación que definen y establecen nuevos límites, espacios, contactos y síntesis.   Vale decir que las líricas de rock cumplen un papel primordial de enlace en la relación comunicación y cultura, puesto que desde sus instancias discursivas, impulsan un mecanismo activador de diálogo, debate e interacción entre los jóvenes y las distintas instancias generacionales de la sociedad. En suma, el trabajo pretende abordar una reflexión sobre el lenguaje y los sentidos explícitos e implícitos que se enraízan en la trama cultural de una sociedad. Se propone así un análisis discursivo de las letras de rock a partir del estudio de los diferentes fenómenos lingüísticos y de los mecanismos de construcción del sentido social. Las piezas discursivas, entonces, se configuran como elementos centrales para comprender el vínculo comunicativo del proyecto.   En este sentido, cobran importancia las estrategias discursivas que se emplean a partir de las líricas de rock, en tanto discurso social, puesto que persiguen proposiciones determinadas que corresponden a la conceptualización y el nombramiento de la libertad.","palabras_clave":"DISCURSO|LIBERTAD |ROCK ARGENTINO|DEMOCRACIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3455,"idioma_id":4,"titulo":"Tracking Trajectory of Underactuated Surface Vessels Based on a Linear Algebra Approach","resumen":"This brief presents the design of a controller that allows an underactuated vessel to track a reference trajectory in the x - y plane. A trajectory tracking controller designed originally for robotic systems is applied for underactuated surface ships. Such a model is represented by numerical methods and, from this approach, the control actions for an optimal operation of the system are obtained. Its main advantage is that the condition for the tracking error tends to zero, and the calculation of control actions are obtained solving a system of linear equations. The proofs of convergence to zero of the tracking error are presented here and complete the previous work of the authors. Simulation results show the good performance of the proposed control system.","palabras_clave":"Control system design|linear algebra|nonlinear model|tracking trajectory control"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3457,"idioma_id":4,"titulo":"Inhibition of the b-carbonic anhydrases from Mycobacterium tuberculosis with C-cinnamoyl glycosides: Identification of the first inhibitor with anti-mycobacterial activity","resumen":"A small series of C-cinnamoyl glycoside containing the phenol moiety was tested for the inhibition of the three Mycobacterium tuberculosis Beta-carbonic anhydrases (CAs, EC 4.2.1.1) with activities in the low micromolar range detected. The compounds were also tested for the inhibition of growth of M. tuberculosis H37Rv strain, leading to the identification of (E)-1-(2´,3´,4´,6´-tetra-O-acetyl-alfa-D-glucopyranosyl)-4-(3-hydroxyphenyl)but-3-en-2-one (1) as the first carbonic anhydrase inhibitor with anti-tubercular activity","palabras_clave":"C-GLYCOSIDES|MYCOBACTERIUM TUBERCULOSIS|CARBONIC ANHYDRASE|ANTI-TUBERCULAR ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3458,"idioma_id":4,"titulo":"Macrophages derived from septic mice modulate nitric oxide synthase and angiogenic mediators in the heart","resumen":"Macrophages (Mps) can exert the defense against invading pathogens. During sepsis, bacterial lipopolisaccharide (LPS) activates the production of inflammatory mediators by Mps. Nitric oxide synthase (NOS) derived-nitric oxide (NO) is one of them. Besides, Mps may produce pro-angiogenic molecules such as vascular endothelial growth factor-A (VEGF-A) and metalloproteinases (MMPs). The mechanisms involved in the cardiac neovascular response by Mps during sepsis are not completely known. We investigated the ability of LPS-treated Mps from septic mice to modulate the behavior of cardiac cells as producers of NO and angiogenic molecules. In vivo LPS treatment (0.1 mg\/mouse) increased NO production more than 4 fold and induced de novo NOS2 expression in Mps. Immunoblotting assays also showed an induction in VEGF-A and MMP-9 expression in lysates obtained from LPS-treated Mps, and in MMP-9 activity detected in cell supernatants. LPS-activated Mps co-cultured with normal heart induced the expression of CD31 and VEGF-A in heart homogenates and increased MMP-9 activity in the supernatants. By immunohistochemistry, we detected new blood vessel formation in hearts cultured with LPS treated Mps. When LPS-stimulated Mps were co-cultured with isolated cardiomyocytes in a transwell assay, the expression of NOS2, VEGF-A and MMP-9 was induced in cardiac cells. In addition, MMP-9 activity was up-regulated in the supernatant of cardiomyocytes. The latter was due to NOS2 induction in Mps from in vivo LPS-treated mice. In conclusion LPS-treated Mps are inducers of inflammatory\/angiogenic mediators in cardiac cells, which could be triggering neovascularization, as an attempt to improve cardiac performance in sepsis.","palabras_clave":"MACROPHAGE|ANGIOGENESIS|SEPSIS|INFLAMMATORY MEDIATORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3459,"idioma_id":4,"titulo":"THE ROLE OF OXIDATIVE STRESS IN ALTERATIONS OF HAEMATOLOGICAL PARAMETERS AND INFLAMMATORY MARKERS INDUCED BY EARLY HYPERCHOLESTEROLEMIA","resumen":"AIMS: The investigation of the effects of a high cholesterol diet (HD) for a short-time period on hematological parameters and the potential role of oxidative stress and inflammation markers.  MAIN METHODS: Rabbits were fed either a control diet or a diet containing 1% cholesterol for 5-6weeks. The plasma lipid levels, C reactive protein (CRP), total red blood cells (RBC), total white blood cells (WBC), platelets count, packed cell volume (PCV) and leukocyte formula were determined. Oxidative stress was evaluated by the thiobarbituric acid reactive substances (TBARS), total glutathione and GSH serum level measurements. The osmotic fragility and the membrane fluidity of erythrocytes were determined. The levels of total cholesterol and TBARS were also measured in the erythrocyte membrane suspension.  KEY FINDINGS: A decrease in the RBC and PCV was observed in rabbits fed on HD. The membrane rigidity and osmotic fragility were increased, and the morphological changes caused by the HD and TBARS levels in the erythrocyte membrane may account for this phenomenon. The inflammatory markers as the CRP levels, the platelets count, the WBC and the neutrophils were increased. The TBARS and GSH levels in the serum were increased and decreased, respectively.  SIGNIFICANCE: This study shows that feeding rabbits an HD for a short time induces hematological alterations, disturbances in the oxidant-antioxidant balance and an increase of inflammatory markers. These findings support the importance of the early correction or prevention of high cholesterol levels to disrupt the process leading to the development of cardiovascular diseases.","palabras_clave":"HYPERCHOLESTEROLEMIA|OXIDATIVE STRESS|BLOOD CELLS|RABBIT|ANEMIA|MEMBRANE FLUIDITY|C REACTIVE PROTEIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3460,"idioma_id":4,"titulo":"Synthesis, physicochemical properties of allopurinol derivatives and their biological activity against Trypanosoma cruzi","resumen":"Chagas disease is caused by Trypanosoma cruzi (T. cruzi) leading to a huge number of infections and deaths per year, because apart from many sufferers only having limited access to health services only an inefficient chemotherapy is available with benznidazol and nifurtimox. Here, C6-alkyl (2a-c) and N1-acyl (3a-c) derivatives of Allopurinol (Allop, compound with activity against T. cruzi) were synthesized in good yields and their structures were unambiguously characterized. Only 2a, 2b and 3c showed inhibitory activity against the proliferative stages of the parasite when tested at 1 µg mL-1 with the 3c derivative exhibiting an IC50 value similar to that of Allop and not being toxic for mammalian cells. Relevant pharmaceutical physicochemical properties (pKa, stability, solubility, lipophilicity) were also determined as well by using Lipinski\u2019s rule, polar surface area and molecular rigidity. Take together, the results demonstrated that the studied derivatives had optimal properties for bioavailability and oral absorption. For the stability studies, Micellar Liquid Chromatography was used as the analytical method which was fully validated according to the FDA guidelines and shown to be a suitable, sensitive and simple method for routine analysis of these Allop derivatives.","palabras_clave":"Trypanosoma cruzi|Allopurinol derivative|Physicochemical properties|Trypanocidal activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3461,"idioma_id":4,"titulo":"Node-by-node disassembly of a mutualistic interaction web driven by species introductions","resumen":"Interaction webs summarize the diverse interactions among species in communities. The addition or loss of particular species and the alteration of key interactions can lead to the disassembly of the entire interaction web, although the nontrophic effects of species loss on interaction webs are poorly understood. We took advantage of ongoing invasions by a suite of exotic species to examine their impact in terms of the disassembly of an interaction web in Patagonia, Argentina. We found that the reduction of one species (a host of a keystone mistletoe species) resulted in diverse indirect effects that led to the disassembly of an interaction web through the loss of the mistletoe, two key seed-dispersers (a marsupial and a bird), and a pollinator (hummingbird). Our results demonstrate that the gains and losses of species are both consequences and drivers of global change that can lead to underappreciated cascading coextinctions through the disruption of mutualisms.","palabras_clave":"biological invasions|community disassembly|seed dispersal|pollination"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3462,"idioma_id":4,"titulo":"Analytical tools for elucidating the biological role of melatonin in plants by liquid chromatography \u2013 tandem mass spectrometry","resumen":"Keywords:Chromatography;Melatonin;MS;Plant material  Melatonin (MT) presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of MT and related compounds in grapes and wines. The analysis of MT in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors; preparation\/cleanup procedures; and chromatographic\/detection parameters, such as HILIC and reverse phase (C8 and C18) chromatographic modes, ESI, and atmospheric pressure chemical ionization (APCI) in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication-mediated extraction step was necessary for grape skin (100% v\/v methanol) and plant tissues (50% v\/v methanol), while wine and must required a SPE preconcentration step. HILIC&#8722;(+) APCI ionization was better for MT standards, while C8&#8722;(+) APCI was the best choice for grape skin and C18&#8722;(+ESI) was suitable for wine. On the other hand, C8&#8722;(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated and the LODs were in the low picogram levels range. The optimized approaches were applied to the determination of MT and its isomer in different vegetal\/food samples; levels found within the range: 4.9?440 ng\/g.","palabras_clave":"Chromatography|melatonin|MS|plant material"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3463,"idioma_id":4,"titulo":"Evaluation and comparison of the ability to predict true linear B-cell epitopes by on-line available prediction programs","resumen":"This work deals with the use of predictors to identify useful B-cell   linear epitopes to develop immunoassays. Experimental techniques to meet   this goal are quite expensive and time consuming. Therefore, we tested 5   free, online prediction methods (AAPPred, ABCpred, BcePred, BepiPred   and Antigenic) widely used for predicting linear epitopes, using the   primary structure of the protein as the only input. We chose a set of 65   experimentally well documented epitopes obtained by the most reliable   experimental techniques as our true positive set. To compare the quality   of the predictor methods we used their positive predictive value (PPV),   i.e. the proportion of the predicted epitopes that are true,   experimentally confirmed epitopes, in relation to all the epitopes   predicted. We conclude that AAPPred and ABCpred yield the best results   as compared with the other programs and with a random prediction   procedure. Our results also indicate that considering the consensual   epitopes predicted by several programs does not improve the PPV.","palabras_clave":"DIAGNOSTIC|EPITOPE|PREDICTION|INMUNOCHEMISTRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3465,"idioma_id":5,"titulo":"Genotipificación de aislamientos clínicos del complejo  Cryptococcus neoformans\/Cryptococcus gattiiobtenidos en el  Hospital «Dr. Julio C. Perrando», de la ciudad de Resistencia  (Chaco, Argentina)","resumen":"La criptococosis es una infección fúngica causada por levaduras del género Cryptococcus, particularmente las del complejo Cryptococcus neoformans\/Cryptococcus gattii. El conocimiento sobre la casuística de la criptococosis en el nordeste argentino es exiguo y no se tiene información sobre los tipos moleculares circulantes. El objetivo de este estudio fue realizar la caracterización genética de los aislamientos pertenecientes al complejo C. neoformans\/C. gattii obtenidos en el Hospital «Dr. Julio C. Perrando» de la ciudad de Resistencia (Chaco, Argentina), con el fin de determinar especie, variedad y genotipo. Durante dos años y un mes se estudiaron 26 aislamientos clínicos. Mediante métodos convencionales y moleculares, un aislamiento fue identificado como C. gattii genotipo VGI y los 25 restantes como C. neoformans var. grubii, 23 de los cuales correspondieron al genotipo VNI y dos al genotipo VNII. Estos datos son una contribución al conocimiento de la epidemiología de la criptococosis en la Argentina y el primer informe sobre genotipos del complejo C. neoformans\/C. gattii de origen clínico en el nordeste argentino.","palabras_clave":"GENOTIPOS|CRIPTOCOCOSIS|EPIDEMIOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3466,"idioma_id":4,"titulo":"Meiotic studies in Brachistosternus alienus Lonnberg 1898 (Scorpiones; Bothriuridae)","resumen":"Brachistosternus Pocock 1893 is the most diverse genus of the scorpion family Bothriuridae. Only four species of the genus have been cytogenetically analyzed so far. We report herein the cytogenetic analysis of Brachistosternus  alienus Lonnberg 1898 from Comallo (R&#305;o Negro province, Argentina). This species is widely distributed in the Monte phytogeographic province, located in central and northern Argentine Patagonia. Meiotic cells of B. alienus from Comallo show 23 homomorphic achiasmatic bivalents. The karyotype of this species contains scarce AT-rich regions that may be associated with the heterochromatin of centromeric regions. Giacomozzi (1977) reports  n 5 14 for B. alienus from Chubut province.  Unfortunately, it is not presently possible to determine if those specimens correspond to B. alienus or to a sympatric species, Brachistosternus angustimanus Ojanguren-Affilastro & Roig-Alsina  2001. These different chromosome numbers of the two populations analyzed may reflect the occurrence of a chromosomal polytypism in B. alienus, or theymay characterize different species.","palabras_clave":"Scorpiones|Citogenetics|Brachistosternus|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3467,"idioma_id":4,"titulo":"LAYERED CRYSTAL STRUCTURE, CONFORMATIONAL AND VIBRATIONAL PROPERTIES OF 2,2,2-TRICHLOROETHOXYSULFONAMIDE: AN EXPERIMENTAL AND THEORETICAL STUDY","resumen":"The molecular structure of 2,2,2-trichloroethoxysulfonamide, CCl3CH2OSO2NH2, has been determined in the solid state by X-ray diffraction data and in the gas phase by ab initio (MP2) and DFT calculations. The substance crystallizes in the monoclinic P21\/c space group with a= 9.969(3) Å, b=22.914(6) Å, c= 7.349(2) Å, &#946;= 91.06(3)°, and Z = 8 molecules per unit cell. There are two independent, but closely related molecular conformers in the crystal asymmetric unit. They only differ in the angular orientation of the sulfonamide (-SO2NH2) group. The conformers are arranged in the lattice as center-symmetric N-H···O(sulf)-bonded dimers. Neighboring dimers are linked through further N-H···O(sulf) bonds giving rise to a crystal layered structure. The solid state infrared and Raman spectra have been recorded and the observed bands assigned to the molecular vibration modes. Also, the thermal behavior of the substance was investigated by TG-DT analysis. The stability of the molecule arising from hyper-conjugative interactions and charge delocalization has been analyzed using natural bond (NBO) analysis.","palabras_clave":"Crystal X-ray diffraction structure|Ab initio MO calculations|Natural bond orbital analysis|Infrared and Raman spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3468,"idioma_id":5,"titulo":"Acción de extracto de Acaena myriophylla sobre componentes celulares y tisulares en heridas cutáneas en ratones","resumen":"Se analizo la acción de Acaena myriophylla sobre componentes tisulares y celulares en heridas netas provocadas en ratones. Las lesiones se trataron durante 10 días obteniendose muestras diarias&nbsp; para realizar estudios histológicos. Los ratones del grupo tratado demostraron en la zona una edematización importante, que determino posiblemente el retrazo en la cicatrización. Al microscopio se evidenció una mayor premanencia de los neutròfilos, el retraso en la aparición y permanencia de los fibroblastos en la zona lesionada y congestión en las etapas iniciales.","palabras_clave":"Acaena |extractos vegetales|cicatrización|piel"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3469,"idioma_id":5,"titulo":"Aprovechamiento del Río Carcarañá-Tercero como Vía Fluvial Navegable. Parte I: Diseño del Sistema de Transporte y de la Vía Fluvial","resumen":"En algunas zonas las carreteras y las vías de ferrocarril son inexistentes o, alternativamente, las rutas y\/o tendidos ferroviarios que existen son inadecuados o insuficientes para el transporte comercial. En esas zonas los ríos potencialmente navegables existentes resultan extremadamente importantes para el transporte de mercancías. El transporte comercial mediante navegación fluvial interior es un importante modo de transporte. En efecto, en la mayoría de las situaciones, en comparación con los otros modos de transporte, tiene la ventaja de menor costo, menor consumo de energía y ahorro de tierras. El objetivo principal del presente trabajo es la realización, a nivel de prefactibilidad, de los estudios necesarios para definir en correspondencia de los ríos Carcarañá-Tercero una ruta fluvial comercialmente navegable. El objetivo propuesto conduce a definir el área de estudio como la zona de influencia del sistema formado por el río Coronda y el río Carcarañá-Tercero, cuyo centro de gravedad puede localizarse en la ciudad de Bell Ville. Aquí se presenta la evaluación de prefactibilidad técnica de un sistema de transporte fluvial integral para esos ríos, haciendo hincapié en el diseño de la vía fluvial y del sistema de transporte.","palabras_clave":"DISEÑO VÍAS NAVEGABLES INTERIORES|TRANSPORTE FLUVIAL|RÍO CARCARAÑÁ|RÍO TERCERO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3470,"idioma_id":4,"titulo":"Chronic stress and its effects on the adrenal cortex apoptosis of pregnant rats","resumen":"The model of chronic intermittent stress by immobilization during pregnancy may produce alter-ations in the mechanisms that maintain adrenal gland homeostasis. In earlier investigations using this model, signii  cant variations in plasma prolactin and corticosterone levels, and adrenal gland weights were observed. We hypothesized that chronic stress causes changes in apoptosis in the adrenal glands of pregnant rats. We identii  ed and quantii  ed apoptotic cells in the adrenal cortex and examined their ultrastructural characteristics using transmission electron microscopy.Adrenal glands of pregnant rats at gestation days 12, 17 and 21 were studied for control and   experimental (stressed) rats. Immunolabelling techniques, stereological analysis and image quan-tii  cation of adrenal gland sections were combined to determine differences in apoptosis in the   different cell populations of the adrenal cortex. The apoptotic index of the experimental rats showed a signii  cant reduction at gestation day 17, while at days 12 and 21 there were no differ-ences from controls. Moreover, the apoptotic index of the reticular zones in control and experi-mental animals showed a signii  cant increase compared to the glomerular and fascicular zones at the three gestation times studied. Chronic stress by immobilization reduced the caspase-depend-  ent apoptotic index at gestation day 17, which may be related to variations in plasma concentra-tions of estrogens and prolactin.","palabras_clave":"ADRENA CORTEX|APOPTOSIS|CASPASA 3|STRESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3471,"idioma_id":4,"titulo":"Medial prefrontal cortex is a cruciaol node of a rapid learning system that retrieves recent and remote memories","resumen":"The neocortex is thought to be a distributed learning system that gradually integrates semantic information into the initial mnemonic representation rapidly formed by the hippocampus after acquisition. Nevertheless, an emerging view suggests that some cortical regions, in particular the medial prefrontal cortex (mPFC), may also have a role during the initial steps of memory consolidation as well as in the recall of recent memories. Here, we show that mPFC plays a critical role during the first few hours of inhibitory avoidance memory consolidation and is necessary for the normal retrieval of both recent and remote memories, supporting the idea that involvement of neocortical areas in memory processing is not restricted to the late post-training consolidation phase","palabras_clave":"mPFC|Memory"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3541,"idioma_id":5,"titulo":"Aporte bibliográfico para nivel medio de Córdoba, variables seleccionadas asociadas a las necesidades básicas","resumen":"RESUMEN DE REVISTA","palabras_clave":"NECESISDADES BASICAS |NIVEL MEDIO |CÓRDOBA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3558,"idioma_id":5,"titulo":"Vulnerabilidad en El Derecho Sucesorio. La mejora al ascendiente y descendiente con discapacidad","resumen":"Se analiza la tutela de los sujetos vulnerables en el derecho sucesorio Argentino vigente y proyectado.","palabras_clave":"Vulnerabilidad.|Discapacidad.|Mejora."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3559,"idioma_id":5,"titulo":"Introducción: el patrimonio compartido, la identidad integral","resumen":"Texto bilingüe (castellano-inglés)BuenosAires es la ciudad en la que vivimos, nuestro lugar, así se convirtió en elpunto de partida de nuestro proyecto y sigue siendo el centro de todo nuestrotrabajo de investigación. En los últimos años hemos estado analizando supatrimonio arquitectónico, en particular el que fue construido a lo largo delsiglo 20, por considerar que es éste el que más presencia tiene en su actualidentidad urbana. De este modo aspiramos a entrenar y capacitar a un grupo decolaboradores docentes y ex-alumnos en la práctica de la investigación y elanálisis crítico para optimizar sus recursos docentes y profesionales.&nbsp;Enlas primeras etapas del proyecto, y de manera de poder profundizar en lainvestigación organizamos el trabajo por capítulos cronológicos. La instanciaactual se focaliza en integrar espacialmente los aportes de diferentes tiemposy comprender a la arquitectura en su sitio y como generadora de espacio urbano,y también en entender al paisaje urbano como la resultante de la convivencia deedificios diferentes y variados aportes culturales que enriquecen sus valorespatrimoniales.&nbsp;Estavez el foco ha sido puesto en la relación entre el patrimonio del siglo 20 enBuenos Aires y aquel de otras ciudades de Argentina. Al hacerlo se han podidoestablecer interesantes vínculos culturales que refieren a una culturaarquitectónica integral pero también definida por su heterogeneidad.&nbsp;Estees, según lo planificado, el último capítulo del presente proyecto deinvestigación, pero no la última experiencia en este campo. Desde 2004 hastahoy dieciocho practicantes han realizado una esperiencia de investigacióndentro del mismo, contribuyendo a un mejor registro y difusión de nuestropatrimonio arquitectónico. Algunos permanecieron por solo un período de un año,un pequeño y entusiasta grupo está desde los inicios, y han ido elevando suscapacidades desde colaboradores en formación hasta investigadores junior ysenior. También cinco autores invitados han iluminado nuestro conocimiento y enriquecidonuestra interpretación acerca de la importancia de un patrimonio tan reciente.&nbsp;Elpatrimonio arquitectónico del siglo 20 en Buenos Aires tiene una presenciarelevante en la ciudad del siglo 21, y también en nuestro propio conceptoproyectual. Estamos preparados para explorar nuevos caminos!","palabras_clave":"PATRIMONIO ARQUITECTONICO|IDENTIDAD|GLOBALIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3560,"idioma_id":5,"titulo":"Derechos de incidencia colectiva, dominio público y responsabilidad del funcionario público en el Proyecto del Código Civil y Comercial Unificado","resumen":"I.- Introducción II. Desarrollo.- 1.- Cuestiones de los derechos de incidencia colectiva, dominio público y responsabilidad del funcionario público.- 1.1.- Derechos de incidencia colectiva: precisiones pretorianas de la legitimación, derechos y cauce procesal en la protección del bien jurídico tutelado.- 1.2.- Dominio Público: las notas típicas de inalienabilidad, imprescriptibilidad e inembargabilidad en los bienes del Estado afectado al interés público.- 1.3.- La responsabilidad del funcionario público: su regulación específica en el artículo 1112 del Código Civil.- 2.- La propuesta del Proyecto del Código Civil y Comercial unificado.- 2.1.- Derechos de incidencia colectiva: el interés jurídico homogéneo como titulo específico de legitimación. 2.2.- Nuevos contornos en el dominio público: el papel de las leyes especiales y el interés público de las obras públicas en la calificación de los bienes. 2.3.- La responsabilidad civil del funcionario público: una materia no delegada por las provincias y susceptible de ser regulada por el derecho público local. 3.- El nuevo Código Civil y Comercial de la Nación.- 3.1.- Los cambios en los derechos de incidencia colectiva: las pautas mínimas para su regulación local en las jurisdicciones de las provincias. 3.2.- Las innovaciones en el dominio Público: las nuevas limitaciones locales, subsidiarias y no indemnizables en el derecho de propiedad. 3.3.- La nueva regulación de la responsabilidad civil del funcionario público: el régimen de derecho público aprobado por la Ley 26.944. III.- Conclusiones","palabras_clave":"Derecho de incidencia colectiva|Dominio Público |Responsabilidad del Funcionario Público |Código Civil y Comercial Unificado"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3561,"idioma_id":4,"titulo":"Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae) a parasitoid wasp used as a biological control agent","resumen":"Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of  fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular  cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n =  20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin  content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using  autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic  short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually  have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this  technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase  chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially  the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding  of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.","palabras_clave":"DIACHASMIMORPHA LONGICAUDATA|PARASITIC HYMENOPTERA|MALE AND FEMALE KARYOTYPES|FISH|CHROMOSOME BANDING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3562,"idioma_id":4,"titulo":"Mild zinc deficiency in male and female rats: early postnatal alterations in renal nitric oxide system and morphology","resumen":"Objective: Fetal and postnatal zinc deficiencies induce an increase in arterial blood pressure and impair renal function in male adult rats.We therefore hypothesized that these renal alterations are present in early stages of life and that there are sexual differences in the adaptations to this nutritional injury. The aim was to study the effects of moderate zinc deficiency during fetal life and lactation on renal morphology, oxidative stress, apoptosis, and the nitric oxide system in male and  female rats at 21 d of life.  Methods: Female Wistar rats received low (8 ppm) or control (30 ppm) zinc diets from the beginning of pregnancy to weaning. Glomerulus number, morphology, oxidative stress, apoptotic cells, nitric oxide synthase activity, and protein expression were evaluated in the kidneys of offspring at 21 d.  Results: Zinc deficiency decreased the nephron number, induced glomerular hypertrophy, increased oxidative damage, and decreased nitric oxide synthase activity in the male and female rat kidneys. Nitric oxide synthase activity was not affected by inhibitors of the neuronal or  inducible isoforms, so nitric oxide was mainly generated by the endothelial isoenzyme. Gender differences were observed in glomerular areas and antioxidant enzyme activities.  Conclusion: Zinc deficiency during fetal life and lactation induces an early decrease in renal functional units, associated with a decrease in nitric oxide activity and an increase in oxidative stress, which would contribute to increased arterial blood pressure and renal dysfunction in  adulthood. The sexual differences observed in this model may explain the dissimilar development of hypertension and renal diseases in adult life.","palabras_clave":"zinc deficiency|gender|renal|nitric oxide"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3563,"idioma_id":4,"titulo":"Environmental enrichment prevents astroglial pathological changes in the hippocampus of APP transgenic mice, model of Alzheimer's disease","resumen":"AbstractAlzheimer's disease (AD) is a neurodegenerative disease that affects neurons and glial cells and leads to dementia. Growing evidence shows that glial changes may precede neuronal alterations and behavioral impairment in the progression of the disease. The modulation of these changes could be addressed as a potential therapeutic strategy. Environmental enrichment has been classically associated to effects on neuronal morphology and function but less attention has been paid to the modulation of glia. We thus characterized astroglial changes in the hippocampus of adult PDAPP-J20 transgenic mice, a model of AD, exposed for 3 months to an enriched environment, from 5 to 8 months of age. Using confocal microscopy, three-dimensional reconstruction and Sholl analysis, we evaluated the morphology of two distinct populations of astrocytes: those associated to amyloid &#946; plaques and those that were not. We found that plaque-associated astrocytes in PDAPP-J20 mice had an increased volume and process ramification than control astrocytes. Non-plaque-associated astrocytes showed a decrease in volume and an increase in the ramification of GFAP+ processes as compared with control astrocytes. Environmental enrichment prevented these alterations and promoted a cellular morphology similar to that found in control mice. Morphological changes in non-plaque-associated astrocytes were found also at 5 months of age, before amyloid &#946; deposition in the hippocampus. These results suggest that glial alterations have an early onset in AD pathogenesis and that the exposure to an enriched environment is an appropriate strategy to reverse them. Cellular and molecular pathways involved in this regulation could constitute potential novel therapeutic targets.","palabras_clave":"ASTROCYTE|HIPPOCAMPUS|TRANSGENIC MICE|ALZHEIMER`S DISEASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3564,"idioma_id":4,"titulo":"Characterization of the hydrochlorothiazide: &#946;-Cyclodextrin inclusion complex. Experimental and theoretical methods","resumen":"Hydrochlorothiazide (HCT) is one of the mostcommonly prescribed antihypertensive drugs. In an attempt togain an insight into the physicochemical and molecular aspectscontrolling the complex architecture of native &#946;-cyclodextrin(&#946;-CD) with HCT, we performed multiple-temperature&#8722;pHisothermal titration calorimetric measurements of the HCT:&#946;-CD system, together with proton nuclear magnetic resonancespectroscopy (1H NMR), phase solubility analysis, and molecularmodeling methods. The AL-type diagrams, obtained at differentpH values and temperatures, suggested the formation ofsoluble 1:1 inclusion complexes of &#946;-CD with HCT. The correspondingstability constants (K1:1) were determined by phasesolubility studies and compared with those obtained by ITC,with good agreement between these two techniques being found. The three-dimensional array of the complex was studied by 1HNMR and molecular modeling methods. Both techniques confirmed the formation of the inclusion complex, with good agreementbetween the experimental and theoretical techniques regarding the HCT binding mode to &#946;-CD. Also, the forces involved in theassociation process were determined, both from the thermodynamic parameters obtained by ITC (association enthalpy, bindingconstant, Gibbs free energy, and entropy) and from energetic decomposition analyses derived from computational methods. Weconcluded that the formation of the HCT:&#946;-CD complex was enthalpy driven, with the inclusion mode of HCT being highlydependent on its ionization state. In all cases, sustained hydrogen bond interactions with hydroxyl groups of &#946;-CD were identified,with the solvation energy limiting the affinity. Regarding the pH and temperature dependence, lower affinity constants were found athigher HCT ionization states and temperatures.","palabras_clave":"HYDROCHLOROTHIAZIDE|BETA- CYCLODEXTRIN|ITC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3565,"idioma_id":4,"titulo":"Loop A is critical for the functional interaction of two Beta vulgaris PIP aquaporins","resumen":"Research done in the last years strongly support the hypothesis that PIP aquaporin can form heterooligomeric assemblies, specially combining PIP2 monomers with PIP1 monomers. Nevertheless, the structural elements involved in the ruling of homo versus heterooligomeric organization are not completely elucidated. In this work we unveil some features of monomer-monomer interaction in Beta vulgaris PIP aquaporins. Our results show that while BvPIP2;2 is able to interact with BvPIP1;1, BvPIP2;1 shows no functional interaction. The lack of functional interaction between BvPIP2;1 and BvPIP1;1 was further corroborated by dose-response curves of water permeability due to aquaporin activity exposed to different acidic conditions. We also found that BvPIP2;1 is unable to translocate BvPIP1;1-ECFP from an intracellular position to the plasma membrane when co-expressed, as BvPIP2;2 does. Moreover we postulate that the first extracellular loop (loop A) of BvPIP2;1, could be relevant for the functional interaction with BvPIP1;1. Thus, we investigate BvPIP2;1 loop A at an atomic level by Molecular Dynamics Simulation (MDS) and by direct mutagenesis. We found that, within the tetramer, each loop A presents a dissimilar behavior. Besides, BvPIP2;1 loop A mutants restore functional interaction with BvPIP1;1. This work is a contribution to unravel how PIP2 and PIP1 interact to form functional heterooligomeric assemblies. We postulate that BvPIP2;1 loop A is relevant for the lack of functional interaction with BvPIP1;1 and that the monomer composition of PIP assemblies determines their functional properties.","palabras_clave":"AQUAPORIN|LOOP A|TETRAMERS|WATER TRANSPORT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3566,"idioma_id":4,"titulo":"Purification and Characterization of AsES Protein. A Subtilisin Secreted by Acremonium strictum is a Novel Plant Defense Elicitor","resumen":"In this work the purification and characterization of an extracellular elicitor protein, designated AsES, produced by an avirulent isolate of the strawberry pathogen Acremonium strictum, is reported. The defense-eliciting activity present in culture filtrates was recovered and purified by ultrafiltration (cut-off 30 kDa), anionic exchange (Q sepharose, pH 7.5) and hydrophobic interaction (Phenyl sepharose) chromatographies. 2D-SDS\/PAGE of the purified active fraction revealed a single spot of 34 kDa and pI 8.8. HPLC (C2\/C18) and MS\/MS analysis confirmed purification to homogeneity. Foliar spray with AsES provided a total systemic protection against anthracnose disease in strawberry, accompanied with the expression of defense-related genes (i.e. PR1, Chi2-1). Accumulation of ROS (e.g. H2O2, O2.-) and callose was also observed in Arabidopsis. By using degenerate primers designed from the partial aminoacid sequences and RACE reactions, the complete AsES-coding cDNA of 1167 nucleotides was obtained. The deduced aminoacid sequence showed significant identity with fungal serine proteinases of the subtilisin family, indicating that AsES is synthesized as a larger precursor containing a 15-residue secretory signal peptide, and a 90-residue peptidase inhibitor I9 domain in addition to the 283-residue of mature protein. AsES exhibited proteolytic activity in vitro and its resistance-eliciting activity was eliminated when inhibited with PMSF, suggesting that its proteolytic activity is required to induce the defense response. This is, to our knowledge, the first report of a fungal subtilisin that shows defense-eliciting activity in plants. This finding could contribute to develop disease biocontrol strategies in plants, by activating its innate immunity.  ","palabras_clave":"Subtilisin|serin-protease|Acremonium strictum|elicitor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3567,"idioma_id":4,"titulo":"New Sylvatic Hosts of Trypanosoma cruzi and Their Reservoir Competence in the Humid Chaco of Argentina: A Longitudinal Study","resumen":"We conducted a cross-sectional survey of Trypanosoma cruzi infection of Triatoma infestans as well as dogsand cats in 327 households from a well-defined rural area in northeastern Argentina to test whether the householddistribution of infection differed between local ethnic groups (Tobas and Creoles) and identify risk factors for hostinfection. Overall prevalence of infection of bugs (27.2%; 95% confidence interval = 25.3?29.3%), dogs (26.0%; 95%confidence interval = 23.3?30.1%), and cats examined (28.7%; 95% confidence interval = 20.2?39.0%) was similar. Amultimodel inference approach showed that infection in dogs was associated strongly with the intensity and duration oflocal exposure to infected bugs and moderately with household ethnic background. Overall, Toba households were at asubstantially greater risk of infection than Creole households. The strong heterogeneities in the distribution of bug, dog,and cat infections at household, village, and ethnic group levels may be used for targeted vector and disease control.","palabras_clave":"Trypanosoma cruzi|reservoir competence|discrete typing unit|sylvatic cycle"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3568,"idioma_id":4,"titulo":"Impairment of Natural Killer (NK) Cells Editing of Immature Dendritic Cells (DC) by Infection with a virulent Trypanosoma cruzi population","resumen":"Early interactions between natural killer (NK) and dendritic cells (DC)   shape the immune response at the frontier of innate and adaptive   immunity. Activated NK cells participate in maturation or deletion of   DCs that remain immature. We previously demonstrated that infection with   a high virulence (HV) population of the protozoan parasite Trypanosoma   cruzi downmodulates DC maturation and T-cell activation capacity. Here,   we evaluated the role of NK cells in regulating the maturation level of   DCs. Shortly after infection with HV T. cruzi, DCs in poor maturation   status begin to accumulate in mouse spleen. Although infection induces   NK cell cytotoxicity and cytokine production, NK cells from mice   infected with HV T. cruzi exhibit reduced ability to lyse and fail to   induce maturation of bone marrow-derived immature DCs (iDCs).   NK-mediated lysis of iDCs is restored by in vitro blockade of the IL-10   receptor during NK-DC interaction or when NK cells are obtained from T.   cruzi-infected IL-10 knockout mice. These results suggest that infection   with a virulent T. cruzi strain alters NK cell-mediated regulation of   the adaptive immune response induced by DCs. This regulatory circuit   where IL-10 appears to participate might lead to parasite persistence   but can also limit the induction of a vigorous tissue-damaging T-cell   response.","palabras_clave":"Dendritic cells|Natural killer cells|Trypanosoma cruzi|NK-DC crosstalk"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3569,"idioma_id":4,"titulo":"Obtaining sexual genotypes for breeding in buffel grass","resumen":"Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultative apomicts with high levels of sexuality is required for performing crosses and plant improvement. The aim of this study was to obtain sexual genotypes from controlled crosses using obligate apomictic cultivars and a sexual line. Twelve putative hybrid F1 plants were selected morphologically and two of them were identified as sexual genotypes by PCR using specific primers for reproductive mechanism. Cytoembryological analysis showed 65.5 and 71.3% meiotic embryo sacs in these plants and their hybrid nature was corroborated by AFLP. Both highly sexual genotypes could be used as female parents in crosses for obtaining improved cultivars of buffel grass.","palabras_clave":"CENCHRUS CILIARIS|FACULTATIVE APOMIXIS|HIBRIDIZATION|FORAGE BREEDING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3570,"idioma_id":4,"titulo":"High-precision UePb zircon age from the Anfiteatro de Ticó Formation: Implications for the timing of the early angiosperm diversification in Patagonia","resumen":"The Baqueró Group is one of the most relevant units regarding the study of the early diversification of  angiosperms in South America. Whereas the age of the upper part of the Group, namely the Punta del  Barco Formation, has been recently dated at 114.67  0.18 Ma, the rest of the unit still lacks precise  dating. In this contribution a CA-TIMS UePb zircon age of 118.23  0.09 Ma for a tuff interlayered with  fossiliferous rocks of the Anfiteatro de Ticó Formation (lower part of the Baqueró Group) is reported. This  age constrains the duration of deposition of the Baqueró Group to approximately 4 Ma and provides new  evidence for the age interpretation of the previously described angiosperm flora and associated pollen  assemblages from this unit, until now interpreted as early Aptian or possibly Barremian in age. The  Aptian age of the Baqueró Group allows a better comparison between the paleofloras from this southernmost  region.","palabras_clave":"Cretaceous|Aptian|Palinology|Paleobotany"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3571,"idioma_id":4,"titulo":"AMINO ACIDS CONTENT AND ACETYLCHOLINESTERASE            INHIBITION OF HUPERZIA SAURURUS INFUSION AND DECOCTION","resumen":"            Context: Huperzia saururus (Lam.) Trevis.  (Lycopodiaceae), an autochthonous plant species in Argentine, is used as memory improver in  traditional medicine. For this reason it was studied and the purified alkaloid  extract showed an important activity on learning and memory. &nbsp;Infusions and decoctions are the most common  way of consume for this species.Objectives: To evaluate the H. saururus infusion and decoction activity  as inhibitors of the acetylcholinesterase (AChE) enzyme and, to determine the  amino acid content in both extracts.Material and methods: Infusion and decoction were purified  by ionic exchange chromatography and analyzed by HPLC-UV, and AChE inhibition  of these extracts was evaluated by using the Ellman method.Results: &nbsp;Infusion and decoction exerted  strong AChE inhibitory activities (IC50= 7.2 ± 0.4 and 22.7 ± 5.6  &#956;g\/mL). Among other nine amino acids, arginine (Arg) was identified in a  concentration greater than 9 mg\/100 g of dried aerial parts in both extracts. Discussion and Conclusion: This high content of Arg  could be considered a contributing factor to the previously activity on memory  demonstrated for the H. saururus alkaloid  extract, in view that Arg is implicated indirectly in mnemonic processes since  it is the precursor in the nitric oxide synthesis. Thus, the central effect of H. saururus could involve two different  mechanisms, the cholinergic one and the nitric oxide pathway.  ","palabras_clave":"ANTICHOLINESTERASE ACTIVITY|ARGININE|HPLC|PLANT EXTRACTS|QUANTITATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3572,"idioma_id":4,"titulo":"FTIR gas-phase kinetic study of the reactions of OH radicals and Cl atoms with unsaturated esters: methyl-3,3-dimethyl acrylate, (E)-ethyl tiglate and methyl-3-butenoate","resumen":"The relative-rate technique has been  used to obtain rates coefficients for the reactions of the unsaturated esters methyl-3,3-dimethyl acrylate, (E)-ethyl tiglate and  methyl-3-butenoate with OH radicals and chlorine atoms at 298 ± 2 K  in synthetic air at a total pressure of (760 ± 10) Torr. The experiments were performed in an  environmental chamber using in situ FTIR detection to monitor the decay of the  esters relative to different reference compounds. The following room  temperaturerate coefficients (in units of cm3 molecule-1 s-1) were obtained:  k1(OH + (CH3)2C=CHC(O)OCH3 ) =(4.46 ±1.05) × 10-11, k2(Cl + (CH3)2C=CHC(O)OCH3)  = (2.78 ± 0.46) × 10-10, k3(OH +CH3CH=C(CH3)C(O)OCH2CH3) = (8.32 ± 1.93) ×  10-11, k4(Cl + CH3CH=C(CH3)C(O)OCH2CH3) =(2.53 ± 0.35) × 10-10, k5(OH +  CH2=CHCH2C(O)OCH3) = (3.16 1 ± 0.57) × 10-11, k4(Cl +CH2=CHCH2C(O)OCH3) = (2.10  ± 0.35) × 10-10 . With the exception of the reaction of Cl with methyl-3,3-dimethyl  acrylate (k2), for which one determination exists in the literature, this study  is the first kinetic study for these reactions under atmospheric pressure.  Reactivity trends are discussed in terms of the effect of the alkyl and ester  groups attached to the double bond on the overall rate coefficients towards OH  radicals. The atmospheric implications of the reactions were assessed by the  estimation of the tropospheric lifetimes of the title reactions.","palabras_clave":"unsaturated esters|rate coefficients|addition reactions|tropospheric chemistry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3573,"idioma_id":5,"titulo":"Adaptación del Cuestionario de Voluntad de Trrabajo a una muestra de universitarios españoles","resumen":"El objetivo del presente estudio es evaluar las propiedades psicométricas del Cuestionario de Voluntad de Trabajo adaptado a una muestra de estudiantes universitarios españoles, tanto en su versión de autoinforme como en la versión destinada a evaluar los pares. Los participantes de este estudio son 194 estudiantes de la Universidad de Barcelona, a los que se aplicaron tanto el cuestionario de Voluntad de Trabajo (CVT) como su versión para evaluar a los pares (CVT-P), así como las subescalas de Perseverancia y Distorsión del Big Factor Questionnaire. Los resultados sugieren la eliminación de dos de los ítems de los cuestionarios CVT y CVT-P. Las correlaciones ítem-total corregidas y la consistencia interna fue adecuada para ambos, y el análisis factorial confirmatorio confirma su unidimensionalidad. La relación entre la escala CVT y las demás medidas criterio es adecuada. En conclusión, podemos afirmar que las escalas CVT y CVT-P obtuvieron unas adecuadas propiedades psicométricas tras la depuración de las mismas.","palabras_clave":"VOLUNTAD DE TRABAJO|PERSEVERANCIA|ADAPTACIÓN DE TESTS|PROPIEDADES PSICOMÉTRICAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3574,"idioma_id":5,"titulo":"Los Petelphilidae (Mammalia, Xenarthra) de la Formación Collón Curá (SALMA, Colloncuresnse, Mioceno medio) de Patagonia (Argentina)","resumen":"319Rev. bras. paleontol. 16(2):319-330, Maio\/Agosto 2013© 2013 by the Sociedade Brasileira de Paleontologiadoi: 10.4072\/rbp.2013.2.12ABSTRACT ? THE PELTEPHILIDAE (MAMMALIA, XENARTHRA) FROM THE COLLÓN CURÁ FORMATION(COLLONCURAN, MIDDLE MIOCENE), ARGENTINA. The Peltephilidae (early Eocene-late Miocene) are a group ofextinct armadillos exclusive from South America, known for having horn-like osteoderms on the nasals and maxillaries. Thispaper discusses a nomenclatorial proposal for anatomical description of these cingulate osteoderms and describes for the fi rsttime specimens of Peltephilidae (Xenarthra, Cingulata) from the Collón Curá Formation (Colloncuran, middle Miocene) ofPatagonia (Argentina). The major change in the nomenclature of the osteoderms in Peltephilidae here proposed is to replace theterm ?central or main fi gure? by ?central longitudinal elevation?. Finally, by analogy with the living species of Dasypodidaeand the extinct Pampatheriidae, some considerations are presented about the relationships between the osteoderm and theoverlying epidermal scale in Peltephilidae. The new specimens are assigned to Peltephilidae gen. et sp. indet., Peltephiluspumilus Ameghino and P. nanus Ameghino. The latter two species represent their youngest record and indicate a closertaxonomic affi nity of the Colloncuran age with the previous Santacrucian, instead of the subsequent Laventan and Mayoan.fi rsttime specimens of Peltephilidae (Xenarthra, Cingulata) from the Collón Curá Formation (Colloncuran, middle Miocene) ofPatagonia (Argentina). The major change in the nomenclature of the osteoderms in Peltephilidae here proposed is to replace theterm ?central or main fi gure? by ?central longitudinal elevation?. Finally, by analogy with the living species of Dasypodidaeand the extinct Pampatheriidae, some considerations are presented about the relationships between the osteoderm and theoverlying epidermal scale in Peltephilidae. The new specimens are assigned to Peltephilidae gen. et sp. indet., Peltephiluspumilus Ameghino and P. nanus Ameghino. The latter two species represent their youngest record and indicate a closertaxonomic affi nity of the Colloncuran age with the previous Santacrucian, instead of the subsequent Laventan and Mayoan.fi gure? by ?central longitudinal elevation?. Finally, by analogy with the living species of Dasypodidaeand the extinct Pampatheriidae, some considerations are presented about the relationships between the osteoderm and theoverlying epidermal scale in Peltephilidae. The new specimens are assigned to Peltephilidae gen. et sp. indet., Peltephiluspumilus Ameghino and P. nanus Ameghino. The latter two species represent their youngest record and indicate a closertaxonomic affi nity of the Colloncuran age with the previous Santacrucian, instead of the subsequent Laventan and Mayoan.Peltephiluspumilus Ameghino and P. nanus Ameghino. The latter two species represent their youngest record and indicate a closertaxonomic affi nity of the Colloncuran age with the previous Santacrucian, instead of the subsequent Laventan and Mayoan.Ameghino and P. nanus Ameghino. The latter two species represent their youngest record and indicate a closertaxonomic affi nity of the Colloncuran age with the previous Santacrucian, instead of the subsequent Laventan and Mayoan.fi nity of the Colloncuran age with the previous Santacrucian, instead of the subsequent Laventan and Mayoan.","palabras_clave":"Xenarthra|Peltephilidae|Mioceno|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3575,"idioma_id":4,"titulo":"Heterogeneity in the antibody response to foot-and-mouth disease primo-vaccinated calves Disease-Infected Cattle","resumen":"Foot-and-mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine-induced protection in cattle has been largely correlated to the FMD virus (FMDV)-specific antibodies. Genetic control of cattle immune adaptive responses has been demonstrated only for peptide antigens derived from FMDV structural proteins. Here, we quantify the heterogeneity in the antibody response of cattle primo-vaccinated against FMD and study its association with the genetic background in Holstein and Jersey sires. A total of 377 FMDV seronegative calves (122 and 255calves from 16 and 15 Holstein and Jersey sires, respectively) were included in the study. Samples were taken the day prior to primo-vaccination and 45 days post-vaccination (dpv). Animals received commercial tetravalent FMD single-emulsion oil vaccines formulated with inactivated FMDV. Total FMDV-specific antibody responses were studied against 3 viral strains included in the vaccine and antibody titers were determined by liquid phase blocking-ELISA. Three linear hierarchical mixed regression models, one for each strain, were formulated to assess the heterogeneity in the immune responses to vaccination. The dependent variables were the antibody titers induced against each FMDV strain at 45 dpv, whereas sire?s ?breed? was included as a fixed effect, ?sire? was included as a random effect and ?farm? was considered as a hierarchical factor to account for lack of independence of within herd measurements. A significant association was found between anti-FMDV antibody responses and sire?s breed, with lower immune responses found in the Jersey sires? offspring compared to those from Holstein sires. No significant intra-breed variation was detected. In addition, farm management practices were similar in this study and results of the serological assays were shown to be repeatable. It therefore seems plausible that differences in the immune response may be expected in the event of a mass vaccination campaigns.","palabras_clave":"FMDV|ANTIBODY RESPONSE|VACCINE|CATTLE|BREED"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":3577,"idioma_id":5,"titulo":"-\t?Estudio de los petroglifos del distrito geológico Cerro Áspero, cuenca alta del río Cuarto, Córdoba, Argentina?","resumen":"Relevamiento petroglifos Cerro Áspero","palabras_clave":"PETROGLIFOS|SIERRA |COMECHINGONES|SUR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":8878,"idioma_id":5,"titulo":"Analfabetismo y Pobreza en San Luis: un estudio descriptivo y comparativo","resumen":"Este trabajo estudia las posibles relaciones entre pobreza y educación enfocándose particularmente en el comportamiento de las variables de Analfabetismo y Analfabetismo Funcional en el aglomerado de San Luis, comparándolas con la Población Económicamente Vulnerada de este aglomerado, la Región de Cuyo y el País. Los principales datos fueron extraídos de la Encuesta Permanente de Hogares correspondiente al Primer Semestre del 2007 y se utilizó el INFOSTAT como procesador. A estos datos se añaden algunos más recientes respecto de la problemática. Los resultados muestran que las variables analfabetismo y analfabetismo funcional en la provincia de San Luis en el 2007 superan levemente la media nacional, pero mantienen homogeneidad con las provincias de la región cuyo. Al trabajar la sub-muestra de la Población Económicamente Vulnerada y compararla con la muestra total de San Luis se observa que el mayor porcentaje de analfabetismo y analfabetismo funcional se concentra en la sub-muestra, lo que estaría dando indicios de la existencia de una relación sustantiva entre pobreza y empeoramiento de las condiciones educativas. Por otra parte, datos más recientes muestran que si bien los índices de analfabetismo han disminuido en la provincia y la región, la relación entre pobreza y empeoramiento de las condiciones educativas sigue siendo significativa.","palabras_clave":"Educación|Analfabetismo|Pobresa|San Luis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":17307,"idioma_id":4,"titulo":"Waste of Molded Pulp Industry.  Filler Panel Production for Construction.","resumen":"In the production process for producing recycled pulp cellulose, there are waste generated by the raw material entering the system. This residue is separated from the pulp by physical methods, and is mainly composed of plastics, paper, laminated papers and other materials in smaller proportions.In order to recycle this waste, in this paper we analyze the viability of use in the production of insulated panels.With the untreated residue extracted from the plant, panels of 30 cm side and thickness of approximately 6 cm were made. On these panels, parameters such as density and compression strength were evaluated for the study of the homogeneity of the material obtained.","palabras_clave":"RESIDUE CELLULOSE INDUSTRY|PANELS|INSULATING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":17308,"idioma_id":4,"titulo":"Lightweight Concrete: an Alternative for Recycling Cellulose Pulp","resumen":"From the analysis of the production process of molded pulp packaging, it can be seen that one of the residues generated in the molding is ?pulp?. When this waste cannot be reincorporated into the production line, is recovered by centrifugation in the effluent treatment system.With the intention of recycling this residue it was investigated the feasibility of their use in the manufacture of concrete blocks or panels lightened studying possible ways to use the material, either in the wet state (form is discharged from the process) or dry (dried in a controlled environment), analyzing the advantages and disadvantages of each alternative.For practical purposes, various dosages were proposed to maintain constant the amount of residue and varying the amounts and proportions of water and cement. Test specimens were made with various amounts of cement per m3 of concrete.","palabras_clave":"CONCRETE|PAPER|RECYCLING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":23277,"idioma_id":5,"titulo":"Aulas virtuales en la Facultad de Ciencias Económicas de la Universidad Nacional de Jujuy. Factibilidad y conveniencia de su implementación","resumen":"Un grupo de docentes pertenecientes a las distintas Áreas Académicas de la Facultad de Ciencias Económicas de la Universidad Nacional de Jujuy con la colaboración de una docente de la Facultad de Humanidades y Ciencias Sociales, formularon un proyecto de investigación con el objeto de determinar si, en dicha Institución, es factible y conveniente implementar aulas virtuales en las materias que integran los currículos de las carreras de grado, a los efectos de desarrollar el proceso educativo bajo la modalidad de enseñanza apoyada con recursos de Internet, Blended Learning. Esta indagación abarca la exploración, la descripción, la explicación y la comprensión de las condiciones existentes en el citado contexto educativo, tomando como categorías de análisis a: la Unidad Académica, los docentes, los estudiantes, los contenidos disciplinares, las aulas virtuales en curso, la comunidad virtual y los egresados. El relevamiento de datos cuanti-cualitativos, su organización e interpretación a través del análisis estadístico  (descriptivo e inferencial, paramétrico y no paramétrico) y cualitativo permitirá formular, a la luz de marco teórico adoptado, un diagnóstico sobre la factibilidad y conveniencia de considerar a las Tecnologías de la Información y la Comunicación como parte del proceso de innovación educativa que hoy debe considerarse en pos de dar respuesta a la emergente ecología conceptual del aprendizaje. Modificar prácticas educativas arraigadas en el tiempo es un proceso complejo. Hacerlo, requiere encarar acciones que garanticen superar con éxito los desafíos implícitos En la presente comunicación abordaremos entonces, los primeros análisis de los avances de investigación desarrollados hasta la fecha.","palabras_clave":"Aulas Virtuales|Factibilidad|Implementación|Ciencias Económicas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":25292,"idioma_id":4,"titulo":"How the fluctuations of water levels affect populations of invasive bivalve Corbicula fluminea (Müller, 1774) in a Neotropical reservoir?","resumen":"Corbicula fluminea is an invasive bivalve responsible for several environmental and financial problems around the globe. Despite the invasive potential of this species, it suffers certain restrictions in lentic environments due to natural phenomena that significantly affect its population structure (e.g. water column fluctuation and sunlight exposure). The present study addresses how temporal decline of the water level in a Neotropical reservoir and exposure to sunlight affect the population structure of C. fluminea. Samplings were carried out twice in the reservoir of Furnas Hydroelectric Power Station (HPS) (Minas Gerais, Brazil), in 2011 and 2012. Population density, spatial distribution and mean shell length of C. fluminea were estimated for each year after sampling in 51 quadrats (0.0625m2) placed on three transects at different distances along the reservoir margins (0, 10 and 20 m from a fixed-point). We observed a predominance of C. fluminea in both years, with a simultaneous gradual decrease in density and richness of native species in the sampling area. Significant differences in density of C. fluminea were registered at different distances from the margin, and are related to the temporal variability of physical conditions of the sediment and water in these environments. We also registered a trend toward an increase in the density and aggregation of C. fluminea as we moved away from the margin, due to the greater stability of these areas (>10 m). The mean shell length of C. fluminea showed significant difference between the distinct distances from the margin and during the years, as well as the interaction of these factors (Distances vs.Years). These results were associated with the reproductive and invasive capacity of this species. This study reveals that these temporal events (especially water column fluctuation) may cause alterations in density, spatial distribution and mean shell length of C. fluminea and the composition of the native malacofauna in Neotropical lentic environments.","palabras_clave":"non-native species|desiccation|water column levels|reservoir"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":27408,"idioma_id":5,"titulo":"La dimensión política en la exposición de las leyes bíblicas de Filón de Alejandría","resumen":"Los tratados Sobre el Decálogo y Las leyes particulares de Filón de Alejandría tienen el propósito de exponer, sistematizar y explicar las numerosas leyes diseminadas en los libros mosaicos. Sin embargo, además de esta labor de carácter exegético, puede afirmarse que en su tratamiento de las leyes bíblicas el filósofo judío persigue también objetivos de índole política, que apuntan a otorgar a la nación judía y sus leyes un posicionamiento de preeminencia y centralidad en relación con las legislaciones existentes entre los demás pueblos. En efecto, si por un lado la Ley bíblica se presenta desde el punto de vista filónico como la más perfecta y ecuánime regulación de las relaciones humanas, en absoluta concordancia con las leyes naturales que rigen el cosmos, por otro lado, las normativas estrictamente religiosas, es decir, las que regulan las relaciones del hombre con Dios, obtienen en la exposición filónica un carácter de universalidad manifiesto en las explicaciones alegóricas del Templo, del atuendo del Sumo sacerdote, de las fiestas y de los sacrificios. Es en este sentido que Filón asigna al pueblo de Israel una función sacerdotal con respecto al resto del género humano, pues éste dirige sus plegarias y sacrificios como representante de la humanidad entera y desempeña la función de modelo y ejemplo de la vida virtuosa acorde a las leyes. En el presente trabajo nos proponemos analizar los pasajes en que Filón expresa estas ideas que, en el contexto imperial romano en que escribe el autor judío, adquieren una particular relevancia política.","palabras_clave":"FILÓN|ALEJANDRÍA|LEY|POLÍTICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":29445,"idioma_id":5,"titulo":"Los hongos comestibles en el Código Alimentario Argentino","resumen":"El Código Alimentario Argentino fue actualizado en 2012. No se exige que los envases con trufas (art. 1254) o los productos elaborados con las mismas (art. 417 y 442) lleven en el rótulo el género y la especie.  Además se permite la comercialización de varios hongos silvestres, lo que genera un grave problema en muchas de los laboratorios bromatológicos del país porque el tema no suele formar parte de las asignaturas de la carrera.","palabras_clave":"Código Alimentario Argentino|hongos silvestres|trufas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":31269,"idioma_id":5,"titulo":"'La autobiografía de Victoria Ocampo, un autorretrato edificante'","resumen":"El artículo presenta una lectura de la Autobiografía de Victoria Ocampo atenta a la figuración de sí misma que la escritora compone en el contexto de los años peronistas. La hipótesis que organiza la lectura afirma que su relato, que cuenta la leyenda de la directora de Sur, esto es, la historia de una mujer, nacida bajo un régimen de costumbres injusto para su género, que padece y desafía las limitaciones morales y culturales de su clase, y que, sin sentirse 'ni preparada ni dotada para semejante empresa, decide invertir su fortuna personal en fundar y dirigir un proyecto cultural ambicioso. Como en todas las leyendas, el personaje principal atraviesa una serie de experiencias, obstáculos, y padecimientos de los que sale invariablemente enriquecido. La narración toma la forma de un arduo camino de perfeccionamiento espiritual, inspirado por el deseo de autoconocimiento y autorrealización de la protagonista. Definido como 'una búsqueda de lo absoluto', el recorrido resulta una suerte de aventura interior orientada a recuperar el equilibrio anímico y moral comprometido durante los años de adolescencia y juventud. Enunciada sobre el final del tercer volumen, la transmutación del amor carnal en potencia espiritual divide la obra en dos momentos diferenciados y complementarios; los primeros tres tomos componen el autorretrato personal de Ocampo, los tres últimos, ordenados según sus encuentros con distintas personalidades de la cultura occidental, presentan los rasgos determinantes de su imagen pública e intelectual.","palabras_clave":"VICTORIA OCAMPO|AUTOBIOGRAFIA|AUTORRETRATO |VIDA EJEMPLAR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":31294,"idioma_id":5,"titulo":"Mi vecino Homi Bhabha","resumen":"En este artículo se indican convergencias y diferencias entre enfoques y conceptos propuestos por Homi Bhabha y mis propias investigaciones. Los ejes temáticos que ordenan este cotejo son los siguientes: 1) Relevancia del lenguaje primario espacial; 2) Umbrales, procesos liminares y contingencia; 3) Configuración del ?tercero?, traducciones y procesos paradójicos; 4) Derecho a significar; 5) Vida cotidiana, sentido común y ponderaciones vecinales.","palabras_clave":"SEMIÓTICA|HOMI BHABHA|PROCESOS LIMINARES|VIDA COTIDIANA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":31322,"idioma_id":5,"titulo":"Responsabilidad del Estado y principios constitucionales","resumen":"Como es sabido, laresponsabilidad del Estado ?nacional, provincial o municipal- en sus múltiplesvariantes ha sido una materia controvertida y largamente discutida en elderecho argentino. Han sido los juecesmediante su labor, de alguna manera creativa, quienes aplicando los principiosgenerales se han convertido en los protagonistas de los cambios más radicalesque ha experimentado esta temática, pasando de una irresponsabilidad prácticamenteabsoluta del Estado a una responsabilidad, incluso, por actos lícitos.Recientemente la Comisión de Derecho de Daños delas XXIV Jornadas Nacionales de Derecho Civil sostuvo, por unanimidad, que laresponsabilidad del Estado por daños se basa sustancialmente en las normas delderecho civil, que pertenecen a la teoría general del derecho, sin perjuicio deque deben considerarse también las características propias de la actividadestatal y los principios&nbsp; y normas delderecho público que la rigen. Comose ha dicho, la responsabilidad del Estado surgede la existencia de un sistema jurídico y político del estado de derecho, caracterizadopor su indispensable sujeción a un régimen normado y al control de los jueces y encuentra sustento en la ConstituciónNacional, los Tratados internacionales y la jurisprudencia de la Corte Supremade Justicia de la Nación","palabras_clave":"Responsabilidad estatal|Principios constitucionales|Responsabilidad civil|Constitución nacional"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":32172,"idioma_id":4,"titulo":"Persistence of measles neutralizing antibody related to vaccine and natural infection adquired before HIV infection","resumen":"Little is known about long-lasting measles protective immunity when exposure to wild type or vaccine measles virus preceded HIV-infection. The results obtained suggest that measles immunity wane and the lowest measles geometric mean antibody titers (GMT) were significantly associated with measles vaccine-induced immunity in individuals that later became HIV-infected (86% prevalence, GMT 164 mIUml) as compared with natural-induced immunity in HIV-infected adults (100% prevalence, GMT 340 mIUml, P= 0?0082) or non-HIV infected adults (100%, GMT 724 mIUml, P= 0?0001) and vaccine-induced immunity in non HIV infected adults (100%, GMT 347 mIUml, P= 0?017). The study was conducted in an area without wild type virus circulation since the year 2000. The absence of virus circulating may alter the paradigm of lifelong immunity to measles virus after vaccination. As the proportion of HIV-infected individuals possessing only vaccine-induced-immunity continues to grow, checking the status of measles immunity in this group is strongly recommended.","palabras_clave":"HIV|INFECCION|SARAMPION|INMUNIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":37343,"idioma_id":5,"titulo":"La Heurística de la Representatividad en un caso de Decisión de Inversión","resumen":"El objetivo de este artículo es evaluar el efecto que tiene la heurística de la representatividad en la percepción de la probabilidad que condiciona un evento en la toma de decisiones en condiciones de riesgo. Para ello, se desarrolla una adaptación ejemplificativa de un experimento presentado por Grether (1980) en un caso hipotético de decisión de inversión de una empresa comercial, empleando el criterio del valor actual neto. Se concluye que el razonamiento bayesiano, el cual arriba a resultados óptimos, no se encuentra presente en la formal real en que los agentes toman decisiones. Así, se demuestra la importancia de estudiar la Economía Conductual, en especial, la heurística de la representatividad, dentro del proceso decisorio de los individuos.","palabras_clave":"Heurística|Representatividad|Toma de decisiones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":37976,"idioma_id":4,"titulo":"Paleogen e Land Mammal Faunas of South America; a Respo nse to Global Climatic Change s and Indigen ous Floral Diversity","resumen":"An appraisal of Paleogene floral and land mammal faunal dynamics in South Americasuggests that both biotic elements responded at rate and extent generally comparable to thatportrayed by the global climate pattern of the interval. A major difference in the SouthAmerican record is the initial as well as subsequent much greater diversity of both Neotropicaland Austral floras relative to North American counterparts. Conversely, the concurrent mammalfaunas in South America did not match, much less exceed, the diversity seen to the north. Itappears unlikely that this difference is solely due to the virtual absence of immigrants subsequentto the initial dispersal of mammals to South America, and cannot be explained solely by thedifferent collecting histories of the two regions. Possible roles plalyed by non-mammalianvertebrates in niche exploitation remain to be explored.The Paleogene floras of Patagonia and Chile show a climatic pattern that approximatesthat of North America, with an increase in both Mean Annual Temperature (MAT) and MeanAnnual Precipitation (MAP) from the Paleocene into the Early Eocene Climatic Optimum(EECO), although the Paleocene-Eocene Thermal Maximum (PETM) is not recognized in theavailable data set. Post-EECO temperatures declined in both regions, but more so in the norththan the south which also retained a higher rate of precipitation.The South American Paleogene mammal faunas developed gradual, but distinct, changesin composition and diversity as the EECO was approached, but actually declined somewhatduring its peak, contrary to the record in North America. At about 40 Ma a post-EECO declinewas recovered in both hemispheres, but the South American record achieved its greatest diversitythen, rather than at the peak of the EECO as in the north. This post-EECO faunal turnoverapparently was a response to the changing conditions when global climate was deteriorating toward the Oligocene. Under the progressively more temperate to seasonally arid conditions inSouth America, this turnover reflected a major change from the more archaic, and more tropicalto subtropical-adapted mammals, to the beginning of the ultimately modern South Americanfauna, achieved completely by the Eocene-Oligocene transition. Interestingly, hypsodonty wasachieved by South American cursorial mammals about 15 ? 20 m.y. earlier than in NorthAmerica. In addition to being composed of essentially different groups of mammals, those of theSouth American continent seem to have responded to the climatic changes associated with theECCO and subsequent conditions in a pattern that was initially comparable to, but subsequentlydifferent from, their North American counterparts.  ","palabras_clave":"South America|Biotic change|Evolution|Paleontology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":38230,"idioma_id":4,"titulo":"The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing","resumen":"Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense,viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, theP0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated thatcotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that wasinduced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamianaplants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana throughAgrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggestingthat the P0 protein exhibits similar RNA silencing suppression activity when expressed from the fulllengthviral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemicsilencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation ofsecondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contributeto understanding the molecular mechanisms involved in the induction of cotton blue disease byCLRDV infection.","palabras_clave":"polerovirus|P0 protein|cotton|silencing supressor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":38703,"idioma_id":4,"titulo":"Possible mechanism of structural transformations induced by StAsp-PSI in lipid membranes","resumen":"In the present work we have analyzed the effect of StAsp-PSI (plant-specific insert of potato aspartic protease) on        the structural and thermotropic properties of the major phospholipid types of bacterial and animal cells. Results        obtained suggest that StAsp-PSI induces a destabilization of the membrane bilayers, depending on the time of        interaction between the protein and the bilayers, rather than on its concentration. This temporal delay would        be consistent with a lateral diffusion of StAsp-PSI monomers to assemble into aggregates to form pores. Like        with the results previously reported for the StAsp-PSI circular dichroism, data obtained here fromIR spectroscopy        show that there are slight changes in the StAsp-PSI secondary structure in the presence of lipid membranes;        suggesting that these changes could be related with the StAsp-PSI self-association. Results obtained from        steady-state fluorescence anisotropy and differential scanning calorimetry assays suggest that StAsp-PSI interacts        with both uncharged and negatively charged phospholipids, modulates the phase polymorphic behavior of        model membranes and partitions and buries differentially in the membrane depending on the presence of        negatively charged phospholipids.","palabras_clave":"ANTIMICROBIAL PROTEIN|ANTITUMOR PROTEIN|PLANT ASPARTIC PROTEASE|PLANT-SPECIFIC INSERT|MEMBRANE DESTABILIZATION|a-HELIX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":38760,"idioma_id":4,"titulo":"Thermal evolution of the mechanical properties of calcareous earthenware","resumen":"Calcareous earthenware is used very frequently for tableware, and fired twice between 980 °C and 1040 °C. Before firing it mostly consists in a kaolinitic clay accompanied by quartz and &#8776;7 wt% of carbonates (Ca and Mg) as fluxing fraction. In this article the firing temperature correlation with the textural, structural and mechanical properties was established in the 700?1100 °C firing range. Materials fired between 800 and 1050 °C presented an interesting correlation between the processing variable (temperature) and the evaluated properties. The porosity in this range was almost constant however the properties evolved exponentially with the temperature. A parallel behavior between flexural strength, diametral compression resistance and dynamic elastic modulus was observed. Furthermore within the technological temperature range the correlation was linear.    Finally the incorporation of the porosimetric analysis performed permitted to understand that the firing processes of calcareous earthenware below 1050 °C is not strictly a sintering process with a gradual densification and loss of porosity: it should be considered as a series of complex chemical processes accompanied by a textural evolution of pore size increase with no important porosity decrease. Only for higher temperature treatments (which exceed the technological ones) the porosity diminishes abruptly. When this reduction takes place, it is accompanied by an increment in the mechanical properties figures and a loss in the dimensional stability.","palabras_clave":"Clays|earthware|Rietveld"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":38783,"idioma_id":4,"titulo":"Galectin-3 controls the response of microglial cells to limit cuprizone-induced demyelination","resumen":"Galectin-3 (Gal-3) is a &#946;-galactoside-binding lectin that plays an important role in inflammatory and neurodegenerative  diseases. Cuprizone (CPZ)-induced demyelination is characterized by the loss of mature oligodendrocytes  (OLG) by apoptosis, myelin sheath degeneration and recruitment of microglia and astrocytes to the  lesioned area. We compared CPZ-induced demyelination of 8-week-old Lgals3&#8722;\/&#8722; vs WT mice. Lgals3&#8722;\/&#8722; mice  displayed a similar susceptibility to CPZ-induced demyelination up to the fifth week, as evaluated byMBP immunostaining  and electronic microscopy. However, OLG progenitors (OPC) generated in CPZ-treated Lgals3&#8722;\/&#8722; mice  showed diminished arborization, suggesting decreased ability of these cells to differentiate. Surprisingly, while  WT mice experienced spontaneous remyelination in the fifth week of CPZ treatment?even though the CPZ  diet was maintained up to sixth week?Lgals3&#8722;\/&#8722; mice lacked this capacity and suffered continuous demyelination  up to the sixth week, accompanied by pronounced astroglial activation. Moreover, after 2 weeks of CPZ  treatment, WT and Lgals3&#8722;\/&#8722; mice showed lower innate anxiety as compared with respective naive mice, but  only CPZ-treated Lgals3&#8722;\/&#8722; mice showed decreased locomotor activity and exhibited spatial working memory  impairment. Expression of Gal-3 increased during CPZ-induced demyelination in microglia but not in astrocytes.  While CPZ-treatedWTmice displayed heightened microglial activation associatedwith ED1 expression and pronounced  upregulation of the phagocytic receptor TREM-2b, this effectwas not observed in CPZ-treated Lgals3&#8722;\/&#8722;  mice which, in spite of showing an increased number of microglia, these cells evidenced caspase-3 activation. Our  results indicate that Gal-3 is expressed in microglial cells to modulate their phenotype, facilitating the onset of  remyelination and OLG differentiation.","palabras_clave":"GALECTIN-3|DEMYELINATION|CUPRIZONE|MICROGLIAL PHENOTYPE|OLIGODENDROGLIAL DIFFERENCIATION|TREM-2b"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":39730,"idioma_id":4,"titulo":"Steady-state target optimization designs for integrating real-time optimization and model predictive control","resumen":"In industrial practice, the optimal steady-state operation of continuous-time processes is typicallyaddressed by a control hierarchy involving various layers. Therein, the real-time optimization (RTO)layer computes the optimal operating point based on a nonlinear steady-state model of the plant. Theoptimal point is implemented by means of the model predictive control (MPC) layer, which typically usesa linear dynamical model of the plant. The MPC layer usually includes two stages: a steady-state targetoptimization (SSTO) followed by the MPC dynamic regulator. In this work, we consider the integration ofRTO with MPC in the presence of plant-model mismatch and constraints, by focusing on the design of theSSTO problem. Three different quadratic program (QP) designs are considered: (i) the standard designthat finds steady-state targets that are as close as possible to the RTO setpoints; (ii) a novel optimizingcontrol design that tracks the active constraints and the optimal inputs for the remaining degrees offreedom; and (iii) an improved QP approximation design were the SSTO problem approximates the RTOproblem. The main advantage of the strategies (ii) and (iii) is in the improved optimality of the stationaryoperating points reached by the SSTO-MPC control system. The performance of the different SSTO designsis illustrated in simulation for several case studies.","palabras_clave":"REAL-TIME OPTIMIZATION|STEADY-STATE OPTIMIZATION|TARGET OPTIMIZATION|CONSTRAINT CONTROL|MODEL PREDICTIVE CONTROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":40888,"idioma_id":5,"titulo":"'La escuela mira a la escuela': enseñar a hacer cine para pensar la escuela","resumen":"Los discursos sobre la escuela contemporánea acuerdan en diagnosticar una institución en crisis: el trabajo docente, el lugar del estudiante, la transmisión de contenidos y la dinámica del aula parecen redefinirse en relación con las actuales transformaciones socio-culturales, generando un extendido clima de incertidumbre. Frente a estos discursos, nos preguntamos cuál será la visión de los estudiantes sobre la escuela. El proyecto La escuela mira a la escuela propone a alumnos de nivel medio la realización de cortometrajes de ficción, con el fin de que puedan relatar historias que tengan como escenario la escuela. Estas producciones constituyen luego fuentes de reflexión sobre sus representaciones acerca de la institución, sus actores, sus modos de funcionamiento. En este artículo presentamos la experiencia realizada en una escuela de Mendoza, deteniéndonos tanto en los fundamentos y objetivos del proyecto como en el modo de trabajo y el análisis de las producciones de los estudiantes.","palabras_clave":"Escuela|Cine|Ficción|Producción Audiovisual"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":41491,"idioma_id":5,"titulo":"Documentación del monasterio de Santo Toribio de Liébana. Pesquisa sobre derechos jurisdiccionales (año 1471)","resumen":"Pesquisa realizada en el marco de un pleito sobre los derechos jurisdiccionales del monasterio de Santo Toribio de Liébana sobre sus vasallos.","palabras_clave":"Documentación|Santo Toribio de Liébana|Pesquisa sobre derechos jurisdiccionales|transcripción paleográfica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":42006,"idioma_id":5,"titulo":"Características de la Superficie de Implantes de Zirconio Sinterizado","resumen":"Los fenómenos biológicos de la oseointegración, se ven influenciados por la superficie de los implantes dentales, cuyas características han ido evolucionando desde superficies relativamente lisas o mecanizadas hasta la formación de superficies rugosas muy especializadas.Se realizaron estudios en estructuras con aposición de plasma, de titanio o hidroxiapatita, o por sustracción de la superficie por chorreado de arena, grabado ácido, o por la combinación de varios tratamientos. En la actualidad se han incorporado áreas de implantes con características, materiales y procesos de producción muy diferentes. Algunas facetas son creadas por tratamientos físicos (ej. rayos láser) que modifican la superficie de titanio de los implantes, otras llamadas bioactivas sugieren la posibilidad de acelerar la oseointegración de los implantes al incorporar diversas sustancias biológicamente activas (ej. flúor); ciertos implantes se han ideado con un nuevo biomaterial denominado zirconio; por último la incorporación de la nanobiología y la nanomedicina impulsan la creación de nanosuperficies en el campo de la implantología oral. La investigación moderna en matertia de implantesm muestra que una superficie rugosa es deseable para aumentar el proceso de oseointegración, puesto que al tornear la rosca en le material de zirconio se produce un acabado relativamente liso. Otros investigadores demostraron una mejor resittencia al torque reverso en implantes de zirconio con superficies porosas en conejos. La propuesta de este trabajo fue caracterizar la superficie de implantes de zirconio sinterizado, a través de microscopía electrónica de barrido. Para ello se utilizaron cinco implantes del biomaterial antes mencionado y se observó la superficie de cada uno de ellos en un microscopio electrónico de barrido marca marca Philips modelo SEM 505. Digitalizador de Imagen SoftImaging Sistem ADDA II, utilizando una magnificación de 180X. Se registraron superficies irregulares con surcos cuya media de profundidad fue de75 &#956;m (ver figura 1). De lo cual se infiere que si bien esta característica permite una correcta adaptación al hueso, sería necesario continuar dicha investigación en otros implantes con rosca para observar la mejor resistencia al torque citada por los autores antes mencionados, en la oseointegración de los mismos.","palabras_clave":"ESMALTE|IMPLANTES|ZIRCONIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":42715,"idioma_id":4,"titulo":"Understanding apple consumers expectations in terms of likes and dislikes: use of comment analysis in a cross- cultural study.","resumen":"Apple consumers expectations in Argentina and France were studied by comment analysis of open-ended questions. In an on-line survey consumers stated: attributes which defined quality in an apple; visual, flavor and texture characteristics they liked\/ did not like to find in an apple. Influence of country, consumption frequency and cultivar knowledge were analyzed by contingency tables, Chi-square per cell tests and Multiple Factor Analysis. Consumers quality expectations were not the same in both countries. For Argentineans quality was more related to visual characteristics; for French it was driven by flavor. However, all agreed that quality apples should be juicy (most used term in both countries), tasty, firm and fresh. Argentineans used more words but French were more specific, particularly for flavour description. Moreover, frequency of consumption, cultivar knowledge and the number of terms given were highly related. Frequent consumers knew more cultivars and were more prolific in relation to flavour. Less frequent consumers knew fewer cultivars and gave more words in the visual category. The use of comment analysis allowed identifying the terms that consumers used in their day to day life to describe apples, finding separately likes and dislikes, in spite of the different languages.","palabras_clave":"Consumer preference | Chi-square per cell|Cross-cultural|Open-ended question|MFACT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":43577,"idioma_id":4,"titulo":"Computational assessment of deep-seated tumor treatment capability of the 9Be(d,n)10B reaction for accelerator-based Boron Neutron Capture Therapy (AB-BNCT)","resumen":"The 9Be(d,n)10B reaction was studied as an epithermal neutron source for brain tumor treatmentthrough Boron Neutron Capture Therapy (BNCT). In BNCT, neutrons are classified according to their energies as thermal (&lt;0.5 eV), epithermal (from 0.5 eV to 10 keV) or fast (&gt;10 keV). For deep-seated tumors epithermal neutrons are needed. Since a fraction of the neutrons produced by this reaction are quite fast (up to 5e6 MeV, even for low-bombarding energies), an efficient beam shaping design is required. This task was carried out (1) by selecting the combinations of bombarding energy and target thickness that minimize the highest-energy neutron production; and (2) by the appropriate choice of the Beam Shaping Assembly (BSA) geometry, for each of the combinations found in (1). The BSA geometry was determined as the configuration that maximized the dose deliverable to the tumor in a 1 h treatment, within the constraints imposed by the healthy tissue dose adopted tolerance. Doses were calculated through the MCNP code.The highest dose deliverable to the tumor was found for an 8 mm target and a deuteronbeam of 1.45 MeV. Tumor weighted doses 40 Gy can be delivered up to about 5 cm in depth,with a maximum value of 51 Gy at a depth of about 2 cm. This dose performance can be improvedby relaxing the treatment time constraint and splitting the treatment into two 1-h sessions.These good treatment capabilities strengthen the prospects for a potential use of this reaction inBNCT.","palabras_clave":"Accelerator-based BNCT|9Be(d,n)10B reaction|Monte-Carlo-simulations|Brain tumor treatment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":43739,"idioma_id":5,"titulo":"Una mirada hacia los ácidos grasos omega 3: aspectos generales e incorporación a productos alimenticios","resumen":"La industrialización, urbanización, desarrollo económico y la globalización del mercado han generadocambios en el estilo de vida de las personas, aumentando el estrés, lo que originó hábitos alimentariosinadecuados y un nivel de actividad física insuficiente para mantener un equilibrio óptimo de energíay un peso saludable; debido a ello, se incrementó la prevalencia de Enfermedades Crónicas NoTransmisibles (ECNT) como obesidad, diabetes, dislipemias, entre otras; constituyendo, actualmente,la principal causa de mortalidad y discapacidad a nivel mundial y que, particularmente, en Argentinaexplican más del 60 % de las muertes","palabras_clave":"ACIDO GRASO OMEGA 3|PRODUCTOS ALIMENTICIOS|SALUDABLE|COLESTEROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44367,"idioma_id":5,"titulo":"Efecto de la congelación sobre la microestructura y las propiedades físicas de películas comestibles a base de proteínas del lactosuero","resumen":"La pérdida de humedad en los alimentos congelados tiene importantes consecuencias económicas y continuamente recibe la atención de los científicos de alimentos principalmente debido al hecho de que la pérdida por exudación durante la descongelación, como resultado de un daño irreversible del tejido durante el proceso de congelación (congelación, almacenamiento y descongelación), conduce a la reducción de la atracción visual y a la pérdida de nutrientes. La pérdida de peso es importante no sólo por el impacto económico de la reducción del peso comercializable, sino también porque la pérdida de humedad está fuertemente relacionada con la alteración de la estructura de los alimentos y, como consecuencia de la textura de los mismos. Las películas y los recubrimientos comestibles podrían constituir una alternativa para mejorar la calidad de los alimentos congelados, principalmente porque pueden reducir la tasa de transferencia de humedad entre el alimento y la atmósfera circundante, mejorar la integridad estructural de los alimentos congelados durante la descongelación, y retrasar la aparición de quemaduras por frío. En el presente trabajo, se&nbsp;evaluó el efecto de la congelación sobre la microestructura y las propiedades físicas de películas comestibles a base de concentrado de proteínas del lactosuero. Si bien las condiciones de obtención y la formulación afectaron las propiedades de las películas, fueron capaces de tolerar bajas temperaturas, y por lo tanto podrían constituir una alternativa interesante para su aplicación en alimentos sometidos al proceso de congelación. ","palabras_clave":"películas comestibles|congelación|proteínas del lactosuero|propiedades físico-químicas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44404,"idioma_id":5,"titulo":"Imágenes en las rocas: uso del espacio y construcción del paisaje mediante el emplazamiento de arte rupestre en dos regiones de Patagonia centro-meridional (Argentina)","resumen":"En este trabajo se presentan resultados preliminares correspondientes a dos regiones arqueológicas de Patagonia centro-meridional (Argentina): Sur del Macizo del Deseado (40 sitios) y Margen Norte del Río Santa Cruz (4.035 m de soporte rocoso relevado mediante 4 transectas). El objetivo principal es caracterizar el paisaje rupestre de cada región a partir de los tipos de motivos, las técnicas de ejecución, los tipos de soporte rocoso y las topografías utilizadas, evaluando tanto la potencial conservación diferencial de las imágenes grabadas y pintadas, derivada de sus distintas rocas soporte y del reparo ofrecido por la morfología de la topografía, como las pautas de producción subyacentes a las mismas. Se comparan los resultadosobtenidos con aquellos publicados por otros autores sobre regiones vecinas marcando semejanzas y diferencias a grandes escalas espaciales, con el objeto de sugerir la existencia de sistemas de comunicación visual y de movilidad de poblaciones cazadoras-recolectoras a escala inter-regional","palabras_clave":"ARTE RUPESTRE|PATAGONIA|CONSTRUCCIÓN DEL PAISAJE|CAZADORES-RECOLECTORES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44408,"idioma_id":5,"titulo":"Arte y espacio. Estructuración de los repertorios de arte rupestre en los cañadones Yaten Guajen y El Lechuza (margen Norte del río Santa Cruz, Patagonia Argentina)","resumen":"El siguiente trabajo presenta un análisis cualitativo y cuantitativo del arte rupestre de los cañadones Yaten Guajen y El Lechuza, pertenecientes a la región Margen Norte del Río Santa Cruz (provincia de Santa Cruz, Argentina). Para cumplir con este objetivo se desarrolló una metodología de relevamiento distribucional diseñada para analizar la distribución del arte en escalas espaciales amplias. Los resultados obtenidos a partir de estos análisis proveen información sobre tendencias generales presentes en el arte rupestre de ambos cañadones en lo referente a su: a) distribución espacial, b) selección de emplazamientos, c) tipos de motivos y d) técnicas ejecución. Los resultados de estos análisis muestran la existencia de ciertos patrones en la construcción visual de las imágenes, que son visibles en los repertorios de tipos de motivos, en sus combinaciones, y en su relación con los tipos de topografía del soporte rocoso. Estos resultados se relacionan luego con la movilidad, comunicación visual y control social de la transmisión de dichos patrones entre los grupos cazadores-recolectores habitantes de la región.","palabras_clave":"ARTE RUPESTRE|MARGEN NORTE DEL RÍO SANTA CRUZ|METODOLOGÍA DISTRIBUCIONAL|TENDENCIAS CUALITATIVAS Y CUANTITATIVAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44586,"idioma_id":4,"titulo":"Elemental gesture dynamics are encoded by song premotor cortical neurons","resumen":"Quantitative biomechanical models can identify control parameters that   are used during movements, and movement parameters that are encoded by   premotor neurons. We fit a mathematical dynamical systems model   including subsyringeal pressure, syringeal biomechanics and   upper-vocal-tract filtering to the songs of zebra finches. This reduces   the dimensionality of singing dynamics, described as trajectories (motor   \u2018gestures\u2019) in a space of syringeal pressure and tension. Here we   assess model performance by characterizing the auditory response   \u2018replay\u2019 of song premotor HVC neurons to the presentation of song   variants in sleeping birds, and by examining HVC activity in singing   birds. HVC projection neurons were excited and interneurons were   suppressed within a few milliseconds of the extreme time points of the   gesture trajectories. Thus, the HVC precisely encodes vocal motor output   through activity at the times of extreme points of movement   trajectories. We propose that the sequential activity of HVC neurons is   used as a \u2018forward\u2019 model, representing the sequence of gestures in song   to make predictions on expected behaviour and evaluate feedback.","palabras_clave":"biomecanica|canto de aves|dinamica no lineal|neurociencia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44676,"idioma_id":4,"titulo":"Do greenhouse experiments predict willow responses to long term flooding events in the field?","resumen":"                        Flooding tolerance should be  included as a trait in breeding programs in forested areas where extreme flooding  events lasting several months can occur. In this context, it is difficult to  carry out controlled, long-term flooding experiments with big trees due to the large  number of specimens to screen. The hypothesis in this work was that a relatively  short-term flooding experiment in a greenhouse would be useful to select clones  capable of enduring long term flooding events in the field. The survival of 4  willow clones in a long term, 12 years field trial that was subjected twice to long  term flooding events was compared with the survival, growth and gas exchange of  those same clones subjected to flooding in the greenhouse for 3 months. There  were differences in flooding survival in the field: clone AN4 showed a 100 %  survival, followed by clone 395 (43 %), clone 131 (18 %) and clone 13-44 (14 %).  In the greenhouse, all plants survived and only flooded plants of clone 13-44  experienced a statistically significant growth and stomatal conductance reduction.  The flood tolerance ranking in the field and in the greenhouse only partially  overlapped, the worst clone in the field could be identified in the short-term  greenhouse experiment, but not the best one. It was not possible to identify  clones with tolerance to long-term flooding episodes in the field. Nevertheless,  the greenhouse results were useful to identify clones with flooding sensibility  and with tolerance to short term flooding episodes.   ","palabras_clave":"Salix spp.|survival|growth|Paraná River Delta"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44882,"idioma_id":4,"titulo":"- APPLICATION OF THE REVISED INTERNATIONAL PROGNOSTIC SCORING SYSTEM FOR MYELODYSPLASTIC SYNDROMES IN ARGENTINEAN PATIENTS","resumen":"We retrospectively analysed a cohort of 371 de novo MDS patients diagnosed between 1981 and 2012. According to the WHO classification, 43 patients were classified as refractory cytopenia with unilineage dysplasia\/ refractory anaemia with ringed sideroblasts, 17 as del(5q) syndrome, 201 as refractory cytopenia with multilineage dysplasia, 44 as RAEB type 1, and 66 as RAEB type 2. The median age was 70 (17?92) years with 72 % of patients above 60, the gender ratio was 1.4 (M\/F: 214\/157), and 157 (42 %) patients showed an abnormal karyotype. During the follow-up (median 23.3 months), 80 (22 %) showedleukemic progression and 161 (43 %) died (median overall survival: 55.5 months). Studied variables (gender and WHO classification, percentage of bone marrow blast, cytogenetic risk category, and depth of cytopenias according to the IPSS-R [2]), and theIPSS [1], IPSS-R (Fig. 1a?b), and IPSS-R adjusted by age (Fig. 1c) [2] systems showed statistical differences for predicting both overall survival and leukemic evolution.","palabras_clave":"myelodysplastic syndromes|International prognostic scoring system|survival"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":44971,"idioma_id":4,"titulo":"Estimating the world's potentially available cropland using a bottom-up approach","resumen":"Previous estimates of the land area available for future cropland expansion relied on global-scale climate, soil and terrain data. They did not include a range of constraints and tradeoffs associated with land  conversion. As a result, estimates of the global land reserve have been high. Here we adjust these estimates for the aforementioned constraints and tradeoffs. We define potentially available cropland as the moderately to highly productive land that could be used in the coming years for rainfed farming, with low to moderate capital investments, and that is not under intact mature forests, legally protected, or already intensively managed. This productive land is underutilized rather than unused as it has ecological or social functions. We also define potentially available cropland that accounts for trade-offs  between gains in agricultural production and losses in ecosystem and social services from intensified agriculture, to include only the potentially available cropland that would entail low ecological and social costs with conversion to cropland. In contrast to previous studies, we adopt a ??bottom-up?? approach by analyzing detailed, fine scale observations with expert knowledge for six countries or regions that are often assumed to include most of potentially available cropland. We conclude first that there is substantially less potential additional cropland than is generally assumed once constraints and trade offs  are taken into account, and secondly that converting land is always associated with significant social and ecological costs. Future expansion of agricultural production will encounter a complex landscape of  competing demands and tradeoffs.","palabras_clave":"agro-ecologycal zone|land reserve|land use|land change|agriculture|food security|degraded land"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":45018,"idioma_id":4,"titulo":"The times are changing: Effects of circadian desynchronization on physiology and disease","resumen":"Circadian rhythms are endogenous and need to be continuously entrained (synchronized) with the environment. Entrainment includes both coupling internal oscillators to external periodic changes as well as synchrony between the central clock and peripheral oscillators, which have been shown to exhibit different phases and resynchronization speed. Temporal desynchronization induces diverse physiological alterations that ultimately decrease quality of life and induces pathological situations. Indeed, there is a considerable amount of evidence regarding the deleterious effect of circadian dysfunction on overall health or on disease onset and progression, both in human studies and in animal models. In this review we discuss the general features of circadian entrainment and introduce diverse experimental models of desynchronization. In addition, we focus on metabolic, immune and cognitive alterations under situations of acute or chronic circadian desynchronization, as exemplified by jet-lag and shiftwork schedules. Moreover, such situations might lead to an enhanced susceptibility to diverse cancer types. Possible interventions (including light exposure, scheduled timing for meals and use of chronobiotics) are also discussed.","palabras_clave":"CIRCADIAN RHYTHMS| ENTRAINMENT|DESYNCRONIZATION|JET-LAG"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":45535,"idioma_id":5,"titulo":"La relación entre química y física: isomerismo óptico  y la paradoja de Hund","resumen":"  En el presente trabajo abordamos el problema de la relación entre química y física focalizándonos en la cuestión del isomerismo óptico y la llamada 'paradoja de Hund', que apunta a la dificultad de dar cuenta de la quiralidad mediante la mecánica cuántica. En particular, presentamos la solución a la paradoja propuesta desde la teoría de la decoherencia. El objetivo del trabajo consiste en cuestionar esta solución a la luz de una interpretación precisa del concepto de decoherencia. Sobre esta base argumentamos que una respuesta satisfactoria a la paradoja de Hund sólo puede brindarse desde una adecuada interpretación de la mecánica cuántica, que logre sortear los desafíos conceptuales de la teoría.","palabras_clave":"PARADOJA DE HUND|ISOMEROS|DECOHERENCIA|RELACIONES INTERTEORICAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":45546,"idioma_id":4,"titulo":"Non-uniform painless decompositions for anisotropic     Besov and Triebel-Lizorkin spaces","resumen":"In this article we construct affine systems that provide a simultaneous atomic decomposition for a wide class of functional spaces including the Lebesgue spaces L p (Rd ), 1 < p < +&#8734;. The novelty and difficulty of this construction is that we allow for non-lattice translations.  We prove that for an arbitrary expansive matrix A and any set &#923;?satisfying a certain spreadness condition but otherwise irregular?there exists a smooth window whose translations along the elements of &#923; and dilations by powers of A provide an atomic decomposition for the whole range of the anisotropic Triebel?Lizorkin spaces. The generating window can be either chosen to be bandlimited or to have compact support.  To derive these results we start with a known general ?painless? construction that has recently appeared in the literature. We show that this construction extends to Besov and Triebel?Lizorkin spaces by providing adequate dual systems.","palabras_clave":"Anisotropic Wavelets|Function spaces|Atomic Decompositions|Beurling Density"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":45804,"idioma_id":5,"titulo":"Consideraciones sobre el pensamiento de Franz Hinkelammert a partir de Teología profana y pensamiento crítico","resumen":"El texto expone algunas consideraciones sobre el pensamiento del filósofo alemán Franz Hinkelammert (1931) emanadas a partir de sus conversaciones mantenidas con la filósofa Estela Fernández Nadal y el sociólogo Gustavo Silnik, ambos mendocinos y estudiosos de su obra. La lectura de Teología profana y pensamiento crítico. Conversaciones con Franz Hinkelammert permite esbozar ciertos interrogantes sobre los procesos sociopolíticos latinoamericanos contemporáneos, revelando de este modo la actualidad y pertinencia del pensamiento de este importante referente del pensamiento crítico latinoamericano.","palabras_clave":"Franz Hinkelammert|Teología Profana|Pensamiento crítico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":45860,"idioma_id":4,"titulo":"Functional Connectivity and Temporal Variability of Brain Connections in Adults with ADHD and Bipolar Disorder","resumen":"Objectives: To assess brain functional connectivity and variability in adults with attention-deficit-hyperactivity disorder (ADHD) or euthymic bipolar disorder (BD) relative to a control group (CT).   Methods: Electroencephalography (EEG) was measured in 35 participants (BD=11; ADHD=9; CT=15) during an eyes-closed 10 minute rest period, and connectivity and graph theory metrics were computed. A coefficient of variation (CV) computed also the connectivity temporal variability of EEG. Multivariate associations between functional connectivity and clinical and neuropsychological profiles were evaluated.   Results: An enhancement of functional connectivity was observed in the ADHD (fronto-occipital connections) and BD (diffuse connections) groups. However, compared with controls, intrinsic variability (CV) was enhanced in the ADHD group and reduced in the BD group. Graph theory metrics confirmed the existence of several abnormal network features in both affected groups. Significant associations of connectivity with symptoms were also observed. In the ADHD group, temporal variability of functional connections was associated with executive function and memory deficits. Depression, hyperactivity and impulsivity levels in the ADHD group were associated with abnormal intrinsic connectivity. In the BD group, levels of anxiety and depression were related to abnormal frontotemporal connectivity.   Conclusions: In the ADHD group, we found that intrinsic variability was associated with deficits in cognitive performance and that connectivity abnormalities were related to ADHD symptomatology. The BD group exhibited less intrinsic variability and more diffuse long-range brain connections, and those abnormalities were related to inter-individual differences in depression and anxiety. These preliminary results are relevant for neurocognitive models of abnormal brain connectivity in both disorders.","palabras_clave":"INTRINSIC VARIABILITY|ATTENTION DEFICIT\/HYPERACTIVITY DISORDER|BIPOLAR DISORDER|ELECTROENCEPHALOGRAPHY|FUNCTIONAL CONNECTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":46872,"idioma_id":5,"titulo":"ANATOMÍA DEL LEÑO DE ZIZIPHUS MISTOL GRISEB. RELACIÓN ALBURA\/DURAMEN","resumen":"Es objetivo del trabajo caracterizar el leño de Ziziphus mistol Griseb., describir la albura y el duramen, estableciendo relaciones en función delDAP (diámetro altura 1.30m), orientación y edad de la planta. Quince arboles (DAP superior a 15cm) pertenecientes a un rodal natural de la localidad SantosLugares, Santiago del Estero, fueron seleccionados al azar y apeados. Las muestras fueron obtenidas a la altura de 1.3 m. Se adoptó la terminología delComité de Nomenclatura de IAWA. El conteo y medición de anillos se efectuó con el Equipo ANIOL y el programa CATRAS. El leño fue analizado anatómicamente, se aplicó el método de la llama y colorantes para la diferenciación albura\/duramen.La madera presenta rasgos anatómicos xeromorfos: porosidad difusa, vasos predominantemente solitarios, pequeños, muy numerosos, con puntuaciones intervasculares alternas, radios 3-1 seriados, heterogéneos, parénquima paratraqueal vasicéntrico, aliforme, en bandas delgadas; fibras cortas con paredes gruesas. El espesor de la albura representa el 75% del diámetro del tronco (intervalo de 80 años), incrementándose con la edad, hasta unvalor de 35 anillos para luego permanecer constante. Comenzado el proceso de duraminización, el paso de albura a duramen no es simultáneo, por lo quehay acumulación de albura. Se proponen modelos de regresión para albura\/duramen y DAP.","palabras_clave":"Madera|Anatomía|Mistol|Duramen|Albura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":46897,"idioma_id":5,"titulo":"SITUACIÓN DE CONSERVACIÓN DE LA PRIMER POBLACIÓN DE ANDIRA INERMIS (FABACEAE) HALLADA EN MISIONES (ARGENTINA)","resumen":"Se da a conocer la presencia de Andira inermis (Fabaceae) en la provincia de Misiones, Argentina. Se presenta un diagnóstico de la situación de conservación de la población hallada, la cual se&nbsp; encuentra diezmada por los efectos de la creciente del río Paraná.","palabras_clave":"Selva riparia|Manduvirá|Flora de Misiones|Recursos naturales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47057,"idioma_id":4,"titulo":"Effect of storage conditions on microbiological and physicochemical parameters of cloudy apple juice concentrate","resumen":"The effects on microbiological parameters of minimally pasteurized cloudy apple juice concentrate obtained in a pilot scale from two apple varieties, and their changes with storage time and temperature were investigated. The juice obtained from Red Delicious apples showed an adequate biological stability for import and export market for storage at -8.5 and -20 ºC during a period of 3 months. The preservation of samples at -20 and -8.5 ºC limited to acceptable values the growth of molds and yeasts during the first two months in the case of juice obtained from Granny Smith apples. Neither lactic acid nor thermo acidophilic bacteria were isolated from the samples in all storage conditions. No noticeable differences in pH and soluble solids content were observed among storage times and temperatures. Turbidity increased slightly with storage time. Viscosity decreased substantially during storage at 2 ºC. Color was stable for all storage conditions evaluated.","palabras_clave":"APPLE JUICE|MICROBIOLOGY|STABILITY|QUALITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47245,"idioma_id":4,"titulo":". Comparative analysis of leaf-litter decomposition from the native Pouteria salicifolia and the exotic invasive Ligustrum lucidum in a lowland stream (Buenos Aires, Argentina)","resumen":"We compared loss in leaf mass and chemical changes (concentrations of carbon, nitrogen, phosphorous,acid detergent fiber, lignin and phenolic compounds) during the decay of submersed leaf-litter of the native Pouteriasalicifolia and the exotic invasive Ligustrum lucidum, in a lowland stream of Buenos Aires (Argentina). Leafinput of L. lucidum was greater than that of P. salicifolia. In addition, leaves of L. lucidum decomposed more rapidlythan those of P. salicifolia, which appear to be related to leaf chemistry, i.e. initial content of carbon, phosphorous,acid detergent fiber, lignin and phenolic compounds and the greatest microbial colonization. Differences in leafchemistry and in the contribution of each plant species to litter changed the quality and quantity of organic matterentering the stream, and therefore influenced differently the process of decomposition. Consequently, we expectthat these changes might alter nutrient cycles and food web dynamics.","palabras_clave":"DECOMPOSITION|EXOTIC TREE|FOOD WEB DYNAMICS|INVASION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47294,"idioma_id":4,"titulo":"Establishment of Chia (Salvia hispanica, Labiateae) in vitro plant and cell cultures","resumen":"We have optimized the culture conditions for the  initiation and maintenance of Salvia  hispanica L. (Chia) callus cultures. Stems were the best explant source for  starting in vitro   cultures. Both IAA  and NAA, at all the concentration tested with or   without the addition of citokinines,  have induced the development of   adventitious roots both in darkness as in  light. In darkness, 2,4-D   (2.25 mM) has induced the  development of S. hispanica    embryogenic tissues and in light promoted the initiation and   establishment of  green, and fast-growing calli that lost their   friability with time. When  subcultured to MS culture media with NAA:kin   (0.54 mM:0.46  mM) or Picloram (1.72 mM, 3.43 mM)    as plant growth regulators, calli maintained friability for two years   in  culture. As for the fat content of the calli obtained, values   remained  significant lower than those of seeds. ","palabras_clave":"cultivo in vitro |embriogenesis somatica|chia|Salvia hispanica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47295,"idioma_id":4,"titulo":"The significance of cytogenetics for the study of karyotype evolution and taxonomy of water bugs (Heteroptera, Belostomatidae) native to Argentina","resumen":"Male meiosis behaviour and heterochromatin characterization of three big water bug species were studied. Belostoma dentatum (Mayr, 1863), B. elongatum Montandon, 1908 and B. gestroi Montandon, 1903 possess 2n = 26 + X1X2Y (male). In these species, male meiosis is similar to that previously observed in Belostoma Latreille, 1807. In general, autosomal bivalents show a single chiasma terminally located and divide reductionally at anaphase I. On the other hand, sex chromosomes are achiasmatic, behave as univalents and segregate their chromatids equationally at anaphase I. The analysis of heterochromatin distribution and composition revealed a C-positive block at the terminal region of all autosomes in B. dentatum, a Cpositive block at the terminal region and C-positive interstitial dots on all autosomes in B. elongatum, and a little C-positive band at the terminal region of autosomes in B. gestroi. A C-positive band on one bivalent was DAPI negative\/CMA3 positive in the three species. The CMA3-bright band, enriched in GC base pairs, was coincident with a NOR detected by FISH. The results obtained support the hypothesis that all species of Belostoma with multiple sex chromosome systems preserve NORs in autosomal bivalents. The karyotype analyses allow the cytogenetic characterization and identification of these species belonging to a difficult taxonomic group. Besides, the cytogenetic characterization will be useful in discussions about evolutionary trends of the genome organization and karyotype evolution in this genus.","palabras_clave":"HETEROPTERA|HOLOKINETIC CHROMOSOMES|KARYOTYPE EVOLUTION MECHANISMS|MULTIPLE SEX CHROMOSOMES|rDNA-FISH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47317,"idioma_id":5,"titulo":"La violencia del silencio, las mujeres de la estepa","resumen":"La violencia de género conlleva elementos de invisibilización y naturalización que usualmente se reconocen en la esfera doméstica. Sin embargo, los estudios de las relaciones generadas a partir de asumir como normal las supremacías de un género sobre el otro, trascienden las vinculaciones familiares y han permitido avances en múltiples perspectivas. Entre ellas vale destacar aquellas que abordan la inequidad desde la construcción del sistema económico falocéntrico (Mellor, 2002), o que avanzan en la problematización vincular con el entorno, dando lugar a la línea teórica desarrollada desde el ecofeminismo (Shiva, 1995, Cavana et al 2004, Puleo, 2011).  En este artículo, exploramos un ejercicio de violencia que se reconoce desde prácticas económicas y modos instrumentales de vinculación con el entorno, enclavados en la estepa patagónica. Este abordaje se debe a que el mismo se legitima desde una coacción silenciada, fundamentada en la lectura que se realiza sobre la geografía. Esto es, desde la construcción e interpretación del paisaje, en donde las prácticas femeninas y feminizadas deben, en la actualidad, luchan por su reconocimiento. Conceptualizamos una original experiencia de organización comunitaria y de producción y comercialización asociativa que interpela históricos\/actuales esquemas sociopolíticos de ordenamiento y desarrollo local y regional.","palabras_clave":"Naturaleza|Economía|Asociativismo|Feminismo|Autonomía"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47339,"idioma_id":4,"titulo":"Effect of aqueous and alcohol extracts of Phytolacca tetramera (Phytolaccaceae) leaves on  Colletotrichum gloeosporioides (Ascomycota)","resumen":"Phytolacca tetramera Hauman ?ombusillo? is an endemism of southeastern Buenos Airesprovince (Argentina). This species has fungicidal action against opportunistic pathogens of humans. Inorder to search natural alternatives for the control of diseases in plants caused by fungi, the objectivewas to evaluate the effects of aqueous and alcohol extracts of P. tetramera leaves on Colletotrichumgloeosporioides (Penz.) Sacc. This fungus has a wide distribution in different species with agricultural,forestry, and ornamental value. The antifungal activity of aqueous and ethanol leaf extracts was assessedin vitro against fungi. The fungus was subjected topes of extracts already incorporated into the PotatoDextrose Agar (PDA) medium at 5-50% concentrations. The aqueous extract concentrations within therange 15-30% led to a decrease in the average diameter and speed of mycelium growth, while the rangeof 15-40% was the most effective in relation to a decrease in conidial production. Also, leaf alcohol extractinhibited the conidial production at concentrations of 5%, and had fungicidal action at concentrations of 15%.From ?ombusillo? leaves a foam index of 250 was obtained. This high concentration of saponins would be atleast one cause of the antifungal activity.","palabras_clave":"antifungal|biocontrol|ombusillo|Phytolacca"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47348,"idioma_id":4,"titulo":"Interactions among resource partitioning, sampling effect, and facilitation on the biodiversity effect: A modeling approach","resumen":"Resource partitioning, facilitation, and samplingeffect are the three mechanisms behind the biodiversityeffect, which is depicted usually as the effect of plantspeciesrichness on aboveground net primary production.These mechanisms operate simultaneously but their relativeimportance and interactions are difficult to unravel experimentally.Thus, niche differentiation and facilitation havebeen lumped together and separated from the samplingeffect. Here, we propose three hypotheses about interactionsamong the three mechanisms and test them using asimulation model. The model simulated water movement through soil and vegetation, and net primary productionmimicking the Patagonian steppe. Using the model, we createdgrass and shrub monocultures and mixtures, controlledroot overlap and grass water-use efficiency (WUE) to simulategradients of biodiversity, resource partitioning andfacilitation. The presence of shrubs facilitated grass growthby increasing its WUE and in turn increased the samplingeffect, whereas root overlap (resource partitioning) had,on average, no effect on sampling effect. Interestingly,resource partitioning and facilitation interacted so the effectof facilitation on sampling effect decreased as resource partitioningincreased. Sampling effect was enhanced by thedifference between the two functional groups in their efficiencyin using resources. Morphological and physiologicaldifferences make one group outperform the other; oncethese differences were established further differences didnot enhance the sampling effect. In addition, grass WUEand root overlap positively influence the biodiversity effectbut showed no interactions.","palabras_clave":"Efecto de Muestreo|Biodiversidad|Facilitación|Partición de Recursos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47384,"idioma_id":4,"titulo":"EFFECT OF COMBINED NATURAL ANTIMICROBIALS ON SPOILAGE MICROORGANISMS AND Listeria innocua IN A WHEY CHEESE ?RICOTTA?","resumen":"The effect of nisin alone and in combination with Microgard? on the natural microbial flora of ricotta cheese and in post-process contamination with Listeria innocua was studied. Microbial groups studied were mesophilic, psychrotrophic, proteolytic, lactic acid and coliform bacteria, and total molds and yeasts. Considering the evolution of all microbial groups and failure criteria adopted for mesophilic and coliform bacteria, this product reached the end of its shelf life before 5 days, which is insufficient time for product commercialization. The combined use of nisin and Microgard? proved to be more effective than the individual application on these microbial groups, resulting in a 13 days shelf life extension. Additionally, the combined treatment reduced the growth of L. innocua as a post-process contamination.","palabras_clave":"COMBINED TREATMENTS  |NATURAL ANTIMICROBIALS  |RICOTTA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47419,"idioma_id":5,"titulo":"Tabaquismo: su influencia sobre la enfermedad periodontal","resumen":"Las enfermedades periodontales son infecciones asociadas a microorganismos patogénicos y alteraciones en la respuesta del huésped. Se desarrollan cuando se produce un desequilibrio entre los microorganismos y los mecanismos de defensa del individuo. Los factores ambientales como el tabaquismo pueden ejercer efectos inmunosupresivos, medioambientales negativos, incrementando la susceptibilidad a periodontitis.La evidencia muestra que hay una clara asociación entre las enfermedades periodontales y el tabaquismo.","palabras_clave":"ENFERMEDAD PERIODONTAL|TABAQUISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47421,"idioma_id":5,"titulo":"Influencia de las conductas adaptativas sobre el estado periodontal","resumen":"Las enfermedades periodontales son infecciones asociadas a microorganismos patogénicos y alteraciones en la respuesta del huésped. Se desarrollan cuando se produce un desequilibrio entre los microorganismos y los mecanismos de defensa del individuo. Los factores psicosociales como el estrés y las conductas de afrontamiento inadecuadas al mismo pueden ejercer efectos inmunosupresivos, incrementando la susceptibilidad a periodontitis. La resiliencia es la capacidad de autosostén, para enfrentar situaciones estresantes, es decir que es la capacidad de encarar los problemas en forma activa &nbsp;con conductas de afrontamiento adecuadas, para atenuar los efectos del estrés y recuperarse superando el problema. La evidencia demuestra que hay una clara asociación entre las enfermedades periodontales y las conductas de afrontamiento frente al estrés.","palabras_clave":"ENFERMEDAD PERIODONTAL|ESTRÉS PSICOSOCIAL|CONDUCTAS DE AFRONTAMIENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47423,"idioma_id":5,"titulo":"El portafolio como herramienta para el mejoramiento de la calidad educativa","resumen":"El trabajo aborda la utilización del portafolio como herramienta para el mejoramiento de la calidad educativa en alumnos universitarios que cursan la carrera de Odontología. En nuestra experiencia, con la utilización de este instrumento, hemos mejorado los resultados de las evaluaciones, y la integración de la disciplina tanto transversal como longitudinal en los tres años del Ciclo de Formación Profesional. ","palabras_clave":"ODONTOLOGÍA|EDUCACIÓN SUPERIOR|PEDAGOGÍA|PORTFOLIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47462,"idioma_id":5,"titulo":"Costos mínimos de compensación y cuantificación de la oferta hídrica en la cuenca alta del río Sauce Grande, Argentina","resumen":"El objetivo deltrabajo fue estimar los costos de aumentar la oferta de disponibilidad hídricaa través del cambio en las prácticas de uso del suelo, para establecer el montomínimo de compensación de un futuro Sistema de Pagos por Servicios Ambientales,dentro del territorio perteneciente a la Cuenca alta del Río Sauce Grande. Se trabajóen tres etapas: simulación de la infiltración para diferentes tormentas através de la aplicación del modelo hidrológico HEC-HMS, cálculo de costos dereconversión de sistemas productivos y cálculo del costo del incremento en laoferta de agua. Como resultados se presentan los mapas temáticos necesariospara simular la infiltración y las tablas que muestran esta variación endistintas recurrencias de tormenta y diferentes prácticas de manejo del suelo.Para la recurrencia 1:1 el costo deoportunidad fue de U$S 1,21.m-3, para la recurrencia de 1:3 fue de U$S0,73.m-3, hasta llegar ala tormenta cuyo costo fue de U$S 0,62.m-3. Se concluye que estoscostos permiten apoyar la planificación del recursohídrico a través de un PSA y que para avanzar se debe realizar unavaloración contingente para establecer la disponibilidad a pagar por losusuarios del servicio ambiental de la cuenca","palabras_clave":"HEC-HMS|servicio ambiental hídrico|cambio de prácticas agropecuarias|márgenes brutos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47487,"idioma_id":5,"titulo":"Propiedades léxicas del entorno lingüístico generadas en clases de Ciencias Sociales en la escuela primaria. Un estudio del vocabulario al que se hallan expuestos los niños","resumen":"El trabajo que se informa tuvo por objeto estudiar las propiedades léxicas del entorno lingüístico (cantidad, diversidad, abstracción y grado de familiaridad del vocabulario) que se generan en las situaciones de enseñanza de CienciasSociales en la escuela primaria. Por medio de un procedimiento cuantitativo se analizó comparativamente la cantidad, la diversidad y la abstracción del vocabulario que configuraba el discurso de las maestras durante las situaciones de enseñanza en primer, tercer y quinto grado de escuelas primarias urbanas y rurales. Asimismo, se elaboró una escala para medir el grado de familiaridadque las palabras empleadas en las clases tenían para los niños. Los resultados de este estudio mostraron que las diferencias más importantes en las propiedades léxicas del entorno lingüístico a que se hallan expuestos los niños a lolargo de la escuela primaria no se encontraron entre primer y tercer grado, sino entre estos grados y quinto grado. Estos resultados no mostraron que los contextos de tercer grado conlleven más oportunidades de aprendizaje de vocabulario que los entornos de primer grado. Esto podría atribuirse a que en los intercambios en las clases de Ciencias Sociales en tercer grado no se observó una descontextualización progresiva de los conocimientos con respecto a primer grado. Los intercambios se centraron, en cambio, en conceptos muy cercanos al entorno de los niños y en vocabulario familiar. Por su parte, no se observaron diferencias entre las escuelas rurales y las escuelas urbanas.","palabras_clave":"vocabulario|clases de ciencias sociales|diversidad|abstracción"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":47864,"idioma_id":4,"titulo":"Handling spectral interferences and matrix effects in DRC-ICPMS to assess the elemental profile in human serum samples after dissolution with formic acid","resumen":"Inductively coupled plasma mass spectrometry instrumentation along with the dynamic reaction cell (DRC) technology has been increasingly used in the last decade for multielemental analysis of biological samples. This work reports the development of a method to assess concentrations of Li, Be, B, Sc, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, As, Se, Rb, Cd, Sr, Mo, Pd, Ag, Sb, Te, Cs, Ba, Os, Tl, Pb, and Bi in human serum samples treated with formic acid. This is a single-step procedure that minimizes sample handling and avoids contamination risks and analyte losses. Towards this aim, several experimental conditions such as sample pretreatment with formic acid and instrumental operating parameters were optimized. Interferences due to polyatomic ions were eliminated using DRC with oxygen as reaction gas through the formation of MO+ ions. Our method involved low dilution factors, and appropriate limits of detection in the low &#956;g L&#8722;1 range for all elements. The accuracy was evaluated through the analysis of a standard reference material (Inorganic Constituents in Animal Serum, NIST 1598a) and spiked samples, with satisfactory recoveries.","palabras_clave":"Elemental profile|Human serum|Formic acid treatment|DRC-ICPMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48050,"idioma_id":4,"titulo":"New contributions to the knowledge of the immatures of Stilobezzia punctulata Lane and Stilobezzia fiebrigi Kieffer (Diptera: Ceratopogonidae)","resumen":"The fourth instar larva of Stilobezzia punctulata  Lane is described for the first time and the one of Stilobezzia fiebrigi  Kieffer is redescribed. They are illustrated and photomicrographed from  material collected in different aquatic environments of &nbsp;the province of Corrientes, Argentina. Both  species show features typical to carnivorous-predatory larva.","palabras_clave":"STILOBEZZIA|CARNIVOROUS|LARVAE|CORRIENTES|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48167,"idioma_id":5,"titulo":"Identidades políticas en la coyuntura latiinoamericana actual. Algunos aportes desde las perspectivas de Ernesto Laclau y Jacques Rancière","resumen":"Esta comunicación presta especial atención a los enfoques teóricos de Jacques Rancière y de Ernesto Laclau, considerando su pertinencia para dar cuenta del clima de cambios que atraviesa América Latina en la última década. Destacamos los aportes de estos autores para analizar las modificaciones en los regímenes comunicativos, los criterios para impartir justicia, y las modas y consumos culturales; como también para abordar la reconfiguración del vínculo entre Estado y movimientos sociales.","palabras_clave":"AMERICA LATINA|GOBIERNOS|MOVIMIENTOS SOCIALES|IDENTIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48200,"idioma_id":5,"titulo":"'Prosperidad y espiritualismo para todos': un análisis sobre la noción de emprendedor en eventos masivos de Buenos Aires","resumen":"El artículo pretende contribuir al análisis del valor del emprendedurismo en las sociedades modernas contemporáneas. A partir del retrato etnográfico de grandes eventos públicos realizados en la ciudad de Buenos Aires vinculados con el emprendedurismo empresarial y las espiritualidades alternativas, se enfatizan aquí dos dimensiones específicas: los valores de la realización personal, concentrados en la búsqueda de prosperidad, y el modo en que los grandes eventos promueven formas novedosas de socialidad entre colectivos identificados con las clases medias.","palabras_clave":"emprendedurismo|Nueva Era|grandes eventos|clases medias de Buenos Aires"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48261,"idioma_id":5,"titulo":"EL HOLOCENO TEMPRANO-MEDIO Y LA RUTA DEL POBLAMIENTO: LAGUNA EL DOCE, DEPARTAMENTO GENERAL LÓPEZ, PROVINCIA DE SANTA FE, ARGENTINA.","resumen":"El trabajo se enmarca en el Proyecto de Investigación ?Estudios arqueológicos de los paisajes  sociales en la Pampa de las Lagunas del sur de Santa Fe (Departamento General López, Provincia  de Santa Fe)? (Código 1HUM361), acreditado en el Instituto de Investigaciones de la Facultad de  Humanidades y Artes de la Universidad Nacional de Rosario. Se dan a conocer cuatro fechados  radiocarbónicos en AMS efectuados sobre materiales arqueológicos (dos fragmentos cerámicos, un resto  óseo humano y uno de guanaco) recuperados en superficie en el sitio Laguna El Doce (San Eduardo,  Departamento General López, Provincia de Santa Fe). Se discuten las características y origen de la  depresión ocupada actualmente por la laguna y su estratigrafía, y se intenta realizar una aproximación  paleoambiental. Los fechados permiten generar nuevas expectativas sobre la ruta del poblamiento y  los eventos de ocupación del sitio, y considerar las estrategias de uso del espacio por parte de grupos  cazadores-recolectores que se desplazaban por la actual Pampa de las Lagunas en la transición entre el  Estadio Isotópico 2 (fines del Pleistoceno) y el Optimo Climático.","palabras_clave":"Arqueología|cazadores recolectores|ambientes lagunares |Holoceno temprano"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48265,"idioma_id":4,"titulo":"Surirella antioquiensis sp. nov . and S. rafaelii sp. nov . (Bacillariophyta) from Colombia","resumen":"Summary: &nbsp;This article is part of a series of studies to  produce and &nbsp;inventory of the diatom  flora of Colombia. Two Surirella taxa S. antioquiensis S.E. Sala, J.J. Ramírez,  Plata-Díaz &amp; Vouilloud and S. rafaelii J.J. Ramírez &amp; &nbsp;S.E. Sala from lotic and lentic aquatic  systems in the Department of Antioquia (Colombia) are proposed as new species.  Both new species were analyzed with light and scanning electron microscopy. Each  taxon is described and illustrated and information about environmental  conditions of sampling sites is given. ","palabras_clave":"Diatoms|Surirella|nov spec|Colombia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48283,"idioma_id":5,"titulo":"?¿Una política democrática de seguridad? Prevención del delito, políticas sociales y disputas en el campo conformado en torno a la inseguridad en la Argentina de la última década?","resumen":"Desde finales de la década de 1970 se observa al interior de la política criminal, fundamentalmente europea, la emergencia de las políticas que se inscriben en el paradigma de la prevención del delito, tanto en su tipo situacional-ambiental como en su modalidad social. En el contexto latinoamericano este desarrollo se produjo durante la década de 1990. Proponemos entender a la estrategia social de prevención del delito como un espacio de intersección entre los campos de la política social y la política criminal, es decir, como ámbito de confluencia y tensión de sus debates, conceptualizaciones, modos de intervención y la producción de sujetos sociales que despliegan. En el presente trabajo nos proponemos analizar las formas de articulación que establecieron los campos de la política social y la política criminal en el desarrollo de este tipo de políticas preventivas en Argentina durante la última década. En este sentido intentaremos sostener una hipótesis histórica: el desarrollo de las políticas preventivas en los inicios de los años 2000 surgió de la articulación estratégica entre la asistencialización de la política social y la emergencia de un proyecto por una ?política democrática de seguridad? centrada en los principios de la prevención y la participación, plasmado en el Plan Nacional de Prevención del Delito. A finales de la década, la relativa centralización de la seguridad social en el campo de la política social y la aparición de un nuevo proyecto por una ?política democrática de seguridad? centrado ahora en la cuestión del control ?político? de las fuerzas de seguridad, marcan el fin de aquella articulación estratégica. Para nuestra tarea, se adoptó un enfoque metodológico cualitativo, combinando el uso de fuentes primarias y fuentes secundarias de diversos tipos, acordes a los diferentes planos de indagación empírica abordados.","palabras_clave":"SEGURIDAD DEMOCRÁTICA|PREVENCIÓN DEL DELITO|POLÍTICA SOCIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48338,"idioma_id":5,"titulo":"DIAFANIZACIÓN Y TINCIÓN DE HUESO Y CARTÍLAGO EN EMBRIONES Y PEQUEÑOS VERTEBRADOS","resumen":"Este trabajo forma parte del Taller de Biología Aplicada&nbsp; que se dicta en&nbsp; la Cátedra de Anatomía Comparada, Facultad de Ciencias Exactas Físicas y Naturales de la Universidad Nacional de Córdoba. República Argentina.Objetivo: Aplicar Técnicas macroscópicas convencionales y nuevas estrategias en la preparación y exhibición de embriones y pequeños vertebrados diafanizados.Materiales y métodos: Se utilizaron un total de seisespecímenes representativos de distintos vertebrados: un juvenil del pez chanchita (Australoherus facetus), dos adultos: un anfibio,&nbsp; la ranita llorona (Physalaemus bilingonigerus)&nbsp; y un reptil, vulgarmente conocido como lagarto gusano (Amphisbaenidae) y tres embriones: tortuga terrestre (Chelonidis chilensis),&nbsp; paloma torcaza (Zenaida auriculata) y comadreja roja (Lutreol inacrassicaudata). El material biológico fue fijado&nbsp; en formol 4%, conservado en alcohol 70% y luego procesado de acuerdo al siguiente protocolo: 1) Identificación del espécimen, 2) Morfometría, 3) Extracción del tegumento, evisceración, remoción de encéfalo y enucleación de los ojos. 4) Lavado, 5) Blanqueamiento en Peróxido de Hidrógeno, 6) Inmersión en una solución de Azul de Alcian para teñir cartílago y postfijación en alcohol 100%, 7) Inmersión en una solución de KHO, a partir del 3% por 24 hs, 8) El tejido óseo se coloreó con el&nbsp;&nbsp; agregado, a la solución de KHO,&nbsp; de 8 gotas de solución acuosa de alizarina roja en 100 ml, controlando&nbsp; la transparencia yel color. 9) Transferencia de las muestras a soluciones de glicerina al 25, 50 y 75% por 24 hs y luego conservación de las mismas en glicerina anhidra pura en un recipiente etiquetado. Resultados y conclusiones: Con el uso de esta técnica se obtuvieron muy buenos resultados en la diafanización de los tejidos, coloración de hueso y cartílago y en la integridad de los&nbsp; ejemplares. Actualmente, este material biológico se conserva en la Cátedra de Anatomía Comparada.","palabras_clave":"EMRIONES Y VERTEBRADOS PEQUEÑOS|TÉCNICA DE DIAFANIZACIÓN|TINCIÓN DE HUESO Y CARTÍLAGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48406,"idioma_id":4,"titulo":"Study of DNA synthesis and mitotic activity of hepatocytes and its","resumen":"  Partial hepatectomy (PH) alters serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. Furthermore, angiogenesis is mainly stimulated by vascular endothelial growth factor (VEGF) and is a fundamental requirement either in liver regeneration or in tumours growth. This study looks at the expression of VEGF, DNA synthesis (DNAs) and mitotic activity (MA) in hepatectomised (H) and hepatectomised-tumour bearing (HTB) mice throughout a 24 h period. Adult male mice were sacrificed every 4 h from 26 to 50 h post-hepatectomy. H mice show a circadian rhythm in VEGF expression with a maximum value of 2.60.1 at 08\/46 h of day\/hours posthepatectomy (HD\/HPH); in DNAs, the maximum value was 3.40.3 at 16\/30 (HD\/HPH) and in MA it was 2.30.01 at 12\/50 (HD\/HPH). In HTB animals the peak of VEGF expression appears at 16\/30 (HD\/HPH) with a maximumvalue of 3.70.1, the peak of DNAs was at 00\/38 (HD\/HPH) with a value of 4.60.3 and the maximum value of MAof 08\/46 (HD\/HPH) with a value of 3.010.3.We can conclude that the presence of the tumour induces modifications in the intensity and the temporal distribution of the circadian curves of VEGF expression, DNAs and MA of hepatectomised animals.ficed every 4 h from 26 to 50 h post-hepatectomy. H mice show a circadian rhythm in VEGF expression with a maximum value of 2.60.1 at 08\/46 h of day\/hours posthepatectomy (HD\/HPH); in DNAs, the maximum value was 3.40.3 at 16\/30 (HD\/HPH) and in MA it was 2.30.01 at 12\/50 (HD\/HPH). In HTB animals the peak of VEGF expression appears at 16\/30 (HD\/HPH) with a maximumvalue of 3.70.1, the peak of DNAs was at 00\/38 (HD\/HPH) with a value of 4.60.3 and the maximum value of MAof 08\/46 (HD\/HPH) with a value of 3.010.3.We can conclude that the presence of the tumour induces modifications in the intensity and the temporal distribution of the circadian curves of VEGF expression, DNAs and MA of hepatectomised animals.","palabras_clave":"cancer|cell cycle|growth factors|immunochemistry|liver\/hepatocytes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48522,"idioma_id":4,"titulo":"Yeasts from autochthonal cheese starters: technological and functional properties","resumen":"Abstract  Aims: The aim of this work was to identify twenty yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties.   Methods and Results: The capacities of the yeasts to grow at different temperatures, pH, NaCl and lactic acid concentrations as well as the proteolytic and lipolytic activities were studied. Moreover, survival to simulated gastrointestinal digestion, hydrophobicity, antimicrobial activity against pathogens and auto- and co- aggregation abilities were evaluated. The sequentiation of a fragment from the 26S rDNA gene indicated that Kluyveromyces marxianus was the predominant species, followed by Saccharomyces cerevisiae, Clavispora lusitaniae, Kluyveromyces lactis and Galactomyces geotrichum. RAPD with primer M13 allowed a good differentiation among strains from the same species. All strains normally grew at pH 4.7 - 5.5 and temperatures between 15 ºC and 35 ºC. Most of them tolerated 10 % NaCl and 3 % lactic acid. Only 40 % and 15 % of isolates were proteolytic and lipolytic, respectively. All strains evidenced high gastrointestinal resistance, moderate hydrophobicity, intermediate auto-aggregation and variable co-aggregation abilities. No strains inhibited the growth of the pathogens assayed.   Conclusions: Some strains from dairy sources showed interesting functional and technological properties.  Significance and Impact of the study: This study has been the first contribution to the identification and characterization of yeasts isolated from cheese autochthonal starters in Argentina. Many strains could be proposed as potential candidates to be used as probiotics and\/or as co-starters in cheese productions.","palabras_clave":"YEASTS|CHEESE|FUNCTIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48576,"idioma_id":4,"titulo":"Ammonia detoxification via ureagenesis in rat hepatocytes involves mitochondrial aquaporin-8 channels","resumen":"          Hepatocyte mitochondrial ammonia detoxification via  ureagenesis is critical for prevention of hyperammonemia and hepatic  encephalopathy. Aquaporin-8 (AQP8) channels facilitate the  membrane transport of ammonia. Since AQP8 is expressed in hepatocyte inner  mitochondrial membranes, we studied whether mitochondrial AQP8 (mtAQP8) plays a  role in ureagenesis from ammonia. Primary cultured rat hepatocytes were  transfected with siRNAs targeting two different regions of the rat AQP8  molecule or with scrambled control siRNA. After 48 h, the levels of mtAQP8  protein specifically decreased by around 80% (P &lt; 0.05) without affecting  cell viability. The mtAQP8-knockdown cells in the presence of ammonium  chloride, showed a decrease in ureagenesis of around 30% (P &lt; 0.05).  Glucagon strongly stimulated ureagenesis in control hepatocytes (+120%, P &lt;  0.05), whereas in mtAQP8-knockdown cells, it induced no significant  stimulation. Contrarily, mtAQP8-silencing induced no significant change in  basal and glucagon-induced ureagenesis when glutamine or alanine was used as  source of nitrogen. NMR studies using 15N-labeled ammonia, confirmed that  glucagon-induced 15N-labeled urea synthesis was markedly reduced in  mtAQP8-knockdown hepatocytes (-90%, P &lt; 0.05). In vivo studies in the rat  showed that under glucagon-induced ureagenesis, hepatic mtAQP8 protein  expression was markedly upregulated (+160% P &lt; 0.05). Moreover, transport  studies in liver inner mitochondrial membrane vesicles showed that glucagon  increased the diffusional permeability to the ammonia analog [14C]methylamine  (+80%, P &lt; 0.05). Conclusion: Hepatocyte mtAQP8 channels facilitate the  mitochondrial uptake of ammonia and its metabolism into urea, mainly under  glucagon stimulation. This mechanism may be relevant to hepatic ammonia  detoxification and in turn, avoid the deleterious  effects of hyperammonemia.  ","palabras_clave":"Urea synthesis|mitochondrial ammonia transport|glucagon|aqua-ammoniaporin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48687,"idioma_id":5,"titulo":"Medición de procesos comunicacionales en organizaciones y proyectos: La dimensión informativa desde la comunicación estratégica","resumen":"El proyecto de investigación y desarrollo Comunicación Estratégica: indicadores comunicacionales en dimensiones múltiples (PID 172 UNR) se propone la construcción de indicadores en cuatro dimensiones de la comunicación: la informativa, la interaccional, la ideológica y la del encuentro sociocultural. Contempla el desarrollo de una plataforma on-line de registro ?en algoritmos fluidos? de los procesos comunicacionales bajo análisis. El abordaje se realiza desde la metaperspectiva de la Comunicación Estratégica de la Escuela de Rosario, que ofrece metodologías específicas de indagación considerando la fluidez y complejidad del fenómeno comunicacional. En este informe se presenta una síntesis del proyecto, avances y resultados parciales que arroja la carga de la dimensión informativa en dos casos (proyecto nacional lechero y proyecto nacional apícola, ambos del Instituto Nacional de Tecnología Agropecuaria de Argentina). Se trata de un desarrollo metodológico disponible para los involucrados en el diseño, planificación y seguimiento de procesos comunicacionales en las organizaciones.","palabras_clave":"COMUNICACION ESTRATEGICA|METODOLOGIA|DIMENSION INFORMATIVA DE LA COMUNICACION|INDICADORES COMUNICACIONALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":48913,"idioma_id":5,"titulo":"Las prácticas jurídicas de perdón: orden social y discursos de la emoción","resumen":"El artículo se sostiene sobre la idea central de que el orden social se inscribe en el cuerpo y que por ende el abordaje de la afectividad debe ocupar un lugar central en los estudios sociales. Desde esta perspectiva, se propone el análisis de ciertas prácticas jurídicas de ?perdón? (construidas sobre discursos de la emoción que estimulan sentencias de inimputabilidad y atenuación de penas) como formas particulares de exhortación social que dirigidas sobre determinados cuerpos, dan cuenta de una configuración de poder particular. Con este propósito, se analizan una serie de fragmentos de expedientes judiciales penales de la jurisprudencia nacional, producidos entre los años 1950 y 1979.","palabras_clave":"ORDEN SOCIAL|PRÁCTICAS JURÍDICAS|PERDÓN|EMOCIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":49018,"idioma_id":4,"titulo":"A revised geographical range for Liolaemus elongatus Koslowsky, 1896 (Squamata: Liolaemini) in Argentina: review of reported and n ew data based distribution with new localities","resumen":"Estimating the effective geographical ranges of species is central to species-oriented conservation and management. In this paper, we review the geographical distribution of Liolaemus elongatus Koslowsky, 1896 with three new records for northern Chubut and southern Río Negro provinces, Argentina. Based on detailed locality records pooled from multiple data sources, including new records obtained for this study, we revise the range of L. elongatus sensu stricto and provide geographical distribution maps comparing the previously recognized range to that proposed herein. Our results show that L. elongatus possesses a much more limited geographic distribution than previously thought, being restricted to areas south of 38°S latitude; the newly proposed range is merely half the species formerly recognized geographical distribution.","palabras_clave":"LIZARD|GEOGRAPHIC RANGE SIZE|BIOGEOGRAPHY|PATAGONIA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":49737,"idioma_id":4,"titulo":"Identification, mapping and evolutionary course of wheat lipoxygenase-1 genes located on the A genomes","resumen":"Durum wheat (Triticum turgidum) is the cereal of preference for semolina   and pasta production. Bright yellow color, which is desirable for pasta   making, depends on the amount of carotenoid pigments present in the   grain. Lipoxygenases (LPXs) account for most of the carotenoid   degradation activity. Although B genome Lpx genes have been extensively   described, little information about the A genome has been reported.   Here, we demonstrate that the Lpx-A1 locus is represented by a single   gene in the diploid T. urartu, the tetraploid T. turgidum and the   hexaploid T. aestivum wheats in contrast to the multiple copies reported   in B genome. The Lpx-A1_like pseudogene previously identified in T.   turgidum genome A was also identified in the T. aestivum cv Chinese   spring wheat, whereas T. urartu possesses a complete copy, suggesting   that pseudogenization occurred after the formation of the tetraploid and   then passes to the hexaploid wheat. The nucleotide sequence of T.   urartu Lpx is more closely related to genome B Lpx-1 than to Lpx genes   of genome A from T. turgidum and T. aestivum, probably due to the   deletions and insertions occurred. Thus, the present paper extends our   knowledge of lipoxygenase gene organization and evolution in the wheat A   genome.","palabras_clave":"LIPOXYGENASE-1| EVOLUTION| PSEUDOGEN|MAPPING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":49814,"idioma_id":4,"titulo":"Grape pomace as a sustainable source of bio-active compounds: extraction, characterization and biotechnological applications of phenolics.","resumen":"Grape pomaces (GP) are characterized by high contents of phenolics due to an incomplete extraction during winemaking process. These phenolics are secondary plant metabolites with potential beneficial effects on human health because of their antioxidant activity and antimicrobial, antiviral and anti-inflammatory properties. Therefore, GP constitutes an inexpensive source for the extraction of phytochemicals that can be used in pharmaceutical, cosmetic and food industries. As a result of the increased attention to sustainability of agricultural practices, efforts have been made to use GP in different fields of industry. Thus, it is necessary to have efficient extraction techniques to achieve good recoveries of compounds. On this respect, sensitive and selective analytical methods have been tried for the characterization of phenolics extracts. This review summarizes the most recent developments in extraction of polyphenols from GP. Furthermore, the techniques used for characterization of extracts are explained, with emphasis in sample preparation, separation and analysis of phenolics. Finally, the possible applications of GP extracts in diverse biotechnological fields are also discussed.","palabras_clave":"Phenolics|Grape"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":49884,"idioma_id":4,"titulo":"Analysis of climate and extrinsic incubation of Dirofilaria immitis in southern South America","resumen":"Dirofilariosis, caused by Dirofilaria immitis and D. repens, is spreading in several geographic regions. The deve - lopment of infective larvae in the mosquito vector (extrinsic incubation) needs an accumulated total of 130 degree-days above the 14 ºC threshold, normally expressed as heartworm development units (HDUs). Based on this information, temperature- based models have been developed and applied to evaluate the distribution and spread of Dirofilaria infections in various countries and continents. Despite the confirmed presence of D. immitis in most South American countries, the available information about its epidemiology remains scarce. We analysed the temporal and spatial extrinsic incubation of this parasite in Argentina, Chile and Uruguay, taking into account daily temperatures from 49 meteorological stations during a 30-year period (1982-2012). The theoretically possible number of D. immitis generations was calculated based on the number of meteorological stations that reached the 130-HDUs threshold. The resulting information was spatially interpolated using the inverse weighted distance (IWD) model to produce thematic maps. The model shows that 41 of the meteorological stations reach the threshold needed and that D. immitis transmission is markedly seasonal with a peak in late spring (December), stable during summer (January to March) and declining in the autumn (April and May). Suitable temperatures exist in Uruguay and most of Argentina, whereas D. immitis transmission in Chile is only possible in the north and in the central inlands. The results suggest that the climatic impact on D. immitis transmission must have been minimal in the countries investigated since the annual meteorological records did not change much during the 30-year period analysed.Keywords","palabras_clave":"Dirofilaria immitis|extrinsic incubation period|geographical information system|temperature|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":50432,"idioma_id":5,"titulo":"El sufrimiento psicosocial en la niñez: el desafío de las políticas en salud mental. Revista Actualidad Psicológica. La Patologización de la Infancia,  pag 21- 23, Marzo de 2013.","resumen":"El aumento progresivo de las problemáticas psicosociales en la niñez ha situado a este grupo poblacional como uno de los de mayor nivel de vulnerabilidad, requiriendo de iniciativas  equitativas y universales para la promoción  y atención de la salud mental, y para hacer frente al estigma, a la discriminación  y a la exclusión que sufren los niños, niñas y adolescentes con sufrimiento psíquico.  Pensar la subjetividad en la niñez y comprender los nuevos modos de padecimiento contemporáneo implica analizar  las profundas transformaciones de la realidad socio-económica  que junto con los cambios culturales y la fragilización de las instituciones, en especial en el ámbito de la familia en las últimas décadas,  generaron cada vez más niños,  niñas  y adolescentes en situación de vulnerabilidad social y fragilidad psíquica.  Numerosos hogares fueron quedando excluidos  como efecto de la creciente  pobreza y el desempleo,  aumentando la brecha de inequidad, que acentuó en nuestro país la desigualdad y la fragmentación social.   La economía capitalista de mercado  promovió la mercantilización de las relaciones sociales  modelando un nuevo tipo de sociabilidad que  debilitó los vínculos y disgregó las formas tradicionales de convivencia. La penetración del individualismo, desregulado de todo encuadre colectivo, impactó en la constitución de las subjetividades produciendo constituciones yoicas cada vez más fracturadas y fragmentadas, sin referentes simbólicos de identidad. Al mismo tiempo, la globalización de los mercados y la implementación de reformas neoliberales estuvo acompañada por una fuerte segmentación en el interior de la sociedad que debilitó las instituciones (Lechner, 1997).   Los dispositivos institucionales que constituían subjetividad, entre ellos los servicios sanitarios, promediando los 90, ya no se encontraban regulados simbólicamente por el Estado, ni satisfacían las demandas de reconocimiento e integración simbólica, contribuyendo de esta manera a generar  un nuevo modo de subjetividad.   Como se sabe, las formas de producción de subjetividad se inscriben en condiciones sociales y culturales específicas y se construye en el encuentro con las instituciones  en las que esa subjetividad se alberga. Es decir no son universales ni atemporales.  El tipo de subjetividad instituida, varía  entonces con las diferentes prácticas de producción, lo que lleva a  interrogarse por las políticas y prácticas de los actores sociales llevadas a cabo en las instituciones sanitarias.   Así como a la necesidad de revisar los discursos y concepciones de la niñez que subyacen a las mismas en tanto impactan de forma positiva o negativa  en los procesos de constitución subjetiva de los niños, niñas y adolescentes.   La década del 90 se caracterizó por la retracción del Estado  en la implementación de las políticas sociales históricamente consensuadas, especialmente en el campo de la salud,  en un contexto de creciente deterioro de las condiciones de vida,   que tuvo  entre otras consecuencias,  la profundización del  nivel de fractura de comunicación entre los servicios sanitarios, y los niños, niñas,  adolescentes y las familias de los hogares más carenciados.   El acceso a los cuidados de la salud pasó de ser un derecho adquirido a ser una ?mercancía?, que era posible comprar según el poder adquisitivo de cada hogar, y  produjo una deconstrucción de la condición de ciudadano como sujeto de derecho para convertirla en otra, la de consumidor\/cliente.   Paradójicamente, en este contexto, la ratificación de la Convención Internacional de los Derechos del Niño en 1990, la Ley N° 114 de la Ciudad y la Ley  nacional N° 2606, - ambas acerca de  Protección Integral de los Derechos del Niño- junto a una legislación progresita en el campo de la  salud mental (Ley N° 448\/2000)  garantizaban el derecho a la salud y promovían la transformación de las políticas de niñez y las prácticas y modelos de intervención pública. Es decir, mientas  esta legislación instalaba un discurso que garantizaba los derechos de ciudadanía, no lograba mitigar el embate de las políticas neoliberales.   Frente a una situación en la que las principales problemáticas giraban en torno a la exclusión social, la marginalidad y la ruptura de lazos sociales, las políticas  y las prácticas de los actores responsabilizaban y atribuían las causas del sufrimiento a los sujetos que los padecían, como una suerte de ?privatización? del dolor. En lugar de afectar  favorablemente en  las condiciones de salud de los niños modificando sus circunstancias adversas  y garantizando el acceso a los cuidados integrales que  atenuaran  el sufrimiento de los mismos,  las políticas de salud mental dirigidas a la niñez en su lugar colaboraron en la génesis y producción de procesos de medicalización del sufrimiento psíquico de los niños, niñas  y adolescentes de la Ciudad de Buenos Aires, dándole características específicas: la exclusión y el  encierro ( Barcala, 2010).  Producto de este escenario, las nuevas demandas que generaban las complejas problemáticas infantiles a los servicios públicos de salud  comenzaron a requerir de una respuesta estatal acorde a los nuevos paradigmas y al marco legislativo vigente.   Este trabajo pretende contribuir  a comprender los modos en que en los últimos años  las políticas de salud mental en la Ciudad  han tomado posición  en relación a un modo de atravesamiento de la niñez que padece por condiciones de alta vulnerabilidad psicosocial. En el mismo sentido, se propone considerar  las respuestas que reciben del Estado, en especial aquéllas ligadas a la institucionalización psiquiátrica. Esto implica conceptualizar las interfaces en un sistema complejo en el que se vinculan elementos de diferente nivel de determinación: el Estado con sus políticas y legislaciones específicas, las instituciones en salud mental, y las prácticas  de los actores sociales institucionales vinculados con la niñez y juventud.","palabras_clave":"Adolescencia|Niñez|sufrimiento|Dispositivos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":50435,"idioma_id":5,"titulo":"Trabajo con Familias.","resumen":"file:\/\/\/C:\/Users\/AleBarcala\/Documents\/Publicaciones%20y%20presentaciones\/Publicaciones%202013\/clepios61.%20Barcala%20.%20Familia.pdf","palabras_clave":"Familias|Niñez|Vulnerabilidad|file:\/\/\/C:\/Users\/AleBarcala\/Documents\/Publicaciones%20y%20presentaciones\/Publicaciones%202013\/clepios61.%20Barcala%20.%20Familia.pdf"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":50492,"idioma_id":4,"titulo":"Alterations of the redox state, pentose pathway and glutathione metabolism in an acute porphyria model. Their impact on heme pathway","resumen":"A classical acute porphyria model in rats consists of combined treatment with 2-allyl-2-isopropylacetamide (AIA) and  3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC). The present work describes the effects of this treatment on the pentose  phosphate (PP) pathway, glutahione metabolism and redox state and how they contribute to alter the glucose pool of  hepatocytes and modulate porphyria, in Wistar rat livers. Our approach is based on the fact that glucose is a repressor of  5-aminolevulinic synthase (ALA-S), the rate-limiting enzyme of the heme pathway, and treatment with AIA\/DCC causes  oxidative stress. Different doses of the xenobiotcs were used. The results show that AIA (500 mg\/kg body weight [BW])\/  DDC (50 mg\/kg [BW]) treatment increased glutathione peroxidase (GPx) activity by 46%, decreased both glutathione  reductase (GR) and glutathione S-transferase (GST) activity by 69% and 52%, respectively, and reduced by 51% reduced  glutathione (GSH) and increased by 100% glutathione disulfide (GSSG) concentrations, therefore lowering by four-fold the  GSH\/GSSG ratio. The activity of glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of PP-pathway,  was increased by 129% as well as that of 6-phosphogluconate dehydrogenase. NADPH and the NADPH\/NADPþ ratio were  increased by 14% and 28%, respectively. These effects could be attributed to the generation of reactive oxygen species  (ROS) elicited by the porphyrinogenic treatment, shown by enhanced DNA damage and ROS production. G6PD stimulation  would decrease hepatic glucose concentrations and consequently exacerbate the porphyria. A decrease in glucose could  stimulate ALA-S and this would add to the effect of drug-induced heme depletion. Since the key role of GST is to  inactivate toxic compounds, the drastic fall in its activity together with the accumulation of ALA would account for the  symptoms of this hepatic disease model. The present findings show the high metabolic interplay between pathways and  constitute a relevant contribution to achieve a better treatment of acute human porphyria.","palabras_clave":"porphyria,|glutathione metabolism|reactive oxygen species|pentose pathway"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":50960,"idioma_id":4,"titulo":"Cheatgrass (Bromus tectorum) invasion in South America: Patagonia under threat?","resumen":"Bromus tectorum (cheatgrass) is an aggressive invasive  species posing threats to native ecosystems including  increase in fire frequency, alteration of water and  nutrient cycles and exclusion of native species. As  such, it is important to monitor this species worldwide.  However, outside the United States, it has been poorly  studied. We studied this species at two scales: (i) at a  local scale, evaluating the species presence and its  determinants, along the ecotonal area between the  steppe and the forest within north-western Patagonia,  to reveal whether B. tectorum is actually invading natural  ecosystems in areas comparable with those  invaded in USA, and (ii) at a regional scale, through a  search of literature and herbaria databases on B. tectorum  in South America, to determine the current known  distribution of the species in this subcontinent. Results  indicate that it is already invading north-western Patagonia  in Argentina, mainly in the semi-arid part of the  region, and that precipitation influences the invasion  process. We found that for South America, B. tectorum  has been only documented in southern Argentina  and Chile. Given the similarities to other invaded  regions, the possibility of invasion for Patagonia has  been predicted but not prevented. It is important to  study changes in the invasion level where it is already  established, and to encourage managers with prevention  and control strategies. Combining this information  with lessons from places with extensive periods of  invasion could help to initiate management of the species  in areas where the invasion process is beginning  and before the species spreads widely.  Keywords: Argentina, cheatgrass, Chile, distribution,  habitat, invasive species, Patagonia.","palabras_clave":"Argentina|Cheatgrass|Chile|Distribution|Invasive species"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51062,"idioma_id":4,"titulo":"Influence of the Brønsted acidity, SiO2\/Al2O3 ratio and Rh-Pd content on the ring opening. Part II. Selective ring opening of methylcyclohexane","resumen":"The influence of the Brønsted acidity, SiO2\/Al2O3 ratio and Rh-Pd content on the ring opening of decalin was studied. Rh- and Pd-based monometallic and bimetallic catalysts were prepared by impregnationon three commercial SiO2\/Al2O3 supports (SIRAL 5, 20 and 40). The catalysts were characterized by TPD of pyridine, X-ray diffraction, H2 chemisorption, isomerization of 3,3-dimethyl-1-butene (33DM1B) and selective ring opening (SRO) of decalin. It was found that the support acidity is strongly influenced by the SiO2\/Al2O3 ratio, the SIRAL 40 being more acid. The metal accessibility has the following order: supported catalysts on SIRAL 40 > SIRAL 20 > SIRAL 5. The incorporation of Rh and\/or Pd modifies the conversion and the product distribution on decalin reaction. The support acidity has a strong influence on the conversionand product distribution on the decalin reaction at 350 °C. SIRAL 5 series have low selectivity to RO and high selectivity to dehydrogenated compounds. On the other hand, SIRAL 40 series lead to the highest yield to cracking products of the three studied series. Catalysts with Rh\/Pd atomic ratios equal to 2 and monometallic Rh1 supported on SIRAL 40 are the most active and selective catalysts to ring opening products.","palabras_clave":"RING OPENING|BRONSTED ACIDITY|BIMETALLIC CATALYSTS |Rh-Pd|BIFUNCTIONAL CATALYSTS|DECALIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51076,"idioma_id":5,"titulo":"Indio Toba no llorando aquel tiempo feliz...Otra vez, Otra vez. De la lucha política al Operativo Toba: las Ligas Agrarias del Nordeste Argentino y el terrorismo de Estado en los años setenta.","resumen":"El golpe de estado del 24 de marzo de 1976 sostenido por una alianza cívico militar se propuso entre otros objetivos transformar las bases económicas y sociales del Estado para re-fundar el pacto de dominación capitalista. Desde esta perspectiva, el artículo se propone historizar el proceso de desarticulación que generó el Estado autoritario en una de los casos de movilización y radicalización política del período como fueron las ligas agrarias en el nordeste de la Argentina. Su politización y radicalización, centradas fundamentalmente en la demanda y el problema del acceso a la tierra, generaron una intensa reacción por parte de los gobiernos de turno y de los sectores económicamente concentrados de la región, quienes activaron una política represiva iniciada, incluso, mucho antes del golpe de estado de 1976. La historia de su surgimiento, apogeo y desarticulación forma parte de un proceso histórico que se inició a mediados de los sesenta y concluyó con la tragedia impuesta por el terrorismo de estado de 1976 que llevó adelante una política de exterminio bajo la denominación de ?Operativo Toba?.","palabras_clave":"Ligas Agrarias|Década del setenta|Terrorismo de Estado|Operativo Toba"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51304,"idioma_id":4,"titulo":"METABOLITES WITH ANTIBIOFILM PROPERTY FROM ASPERGILLUS PARASITICUS (ABSTRACT)","resumen":"modify the concentrations of fungal metabolite (FM) produce by entomopathogenic fungus. The objective was determined the antibacterial and antibiofilm activity of FM produced by Aspergillus parasiticus MEA1 on Pseudomonas aeruginosa ATCC 27853. The fungus was cultured on a dextrose potato broth in the absence (A) and in the presence of 2% (w\/v) cuticle of Spodoptera frugiperda (B), using as a control the medium with insect (C). After 15 days incubation, the supernatants was separated of the mycelium by filtration, both were extracted with chloroform and the FM produce in the different media were analyzed by TLC and GC-MS. The extracts chloroform (EC) obtained from B condition were separated by TLC, and the antibacterial and antibiofilm activity determined. In the EC of the mycelium B the main metabolite found was 2-oleilglicerol (28.67%). On the other hand, the EC of liquid medium had a higher concentration of undecyl-4-fluorobenzoate. The EC mycelium B inhibited 41% the biofilm formation of P. aeruginosa at 100 µg\/mL and the three elautes obtained by TLC preparative:   less-polar (contained 12% of 17-pentatriaconteno), intermediate-polarity  (comprising 35% of 2-(4-bromophenyl) -2-oxoethylbenzoate), and polar-eluate (60% ergosterol) inhibiting  43, 20 and 30% the biofilm formation. The presence of insects induced the formation of FM able to inhibit the biofilm of human pathogen P. aeruginosa; these FM should be evaluated as potential antipathogenics.","palabras_clave":"BIOFILM|Aspergillus parasiticus|Pseudomonas aeruginosa|FUNGAL METABOLITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51438,"idioma_id":4,"titulo":"Argentinian media and journalists enhancing and polluting of communication on Twitter","resumen":"In this research, we look into the behavior of Twitter users in Argentina, both corporate ones (media outlets) and personal ones (journalists), with the aim of establishing the tendency of the first group to work under the traditional ways of communication (one-way, asymmetrical, on a massive scale, underlining the act of saying), and if, on the other hand, the latter tends to adopt innovative ways to express themselves (conversational, symmetrical, personalized, balancing the act of saying and listening). We seek to stipulate how the media and its journalists use Twitter: as a suggestion to engage into a more participative way of communication or as yet another channel of massive distribution of information. An indicator of enhancing\/polluting of communication (here understood as a public conversation) has been designed based on the distinction between the concepts of communication and information, and also of the conventional linguistic and pragmatic structure of conversation.","palabras_clave":"Social Networks|Conversation|Journalism|Twitter"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51451,"idioma_id":4,"titulo":"A Chiral Bis(arsine) Ligand: Synthesis and Applications in Palladium-Catalyzed Asymmetric Allylic Alkylations","resumen":"The new chiral bisarsine-ligand 1,2-bis-N-[2`-diphenylarsinobenzoyl]-1(R),2(R)-diaminecyclohexane (BiAsBA, 3) based on the backbone of the Trost modular ligand (TML) was synthesized in three steps. A useful approach to introduce the -AsPh2 group on arsine ligands by Pd-catalyzed arsination was used. The molecular structure and the configuration of BiAsBA ligand was determined by single-crystal X-ray crystallography.&nbsp; In the asymmetric allylic alkylation of 1,3-diphenyl-2-propenyl acetate with dimethyl malonate very high to complete conversion and modest enantioselectivity were achieved. Despite the low enantioselectivity obtained, the bisarsine ligand BiAsBA showed significant potential, since it provided a higher ee value than the phosphorus-containing homologous Trost Standard Ligand (TSL) with the same substrate.","palabras_clave":"Arsine Chiral Ligand |Asymmetric Allylic Alkylation |Trost Ligand |Pd-catalyzed arsination"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51470,"idioma_id":4,"titulo":"Corrosion of steel drums containing cemented ion-exchange resins as intermediate level nuclear waste","resumen":"Exhausted ion-exchange resins used in nuclear reactors are immobilized by cementation before being stored.&nbsp; They are contained in steel drums that may undergo internal corrosion depending on the presence of certain contaminants.&nbsp; The objective of this work is to evaluate the corrosion susceptibility of steel drums in contact with cemented ion-exchange resins with different aggressive species. The corrosion potential and the corrosion rate of the steel, and the electrical resistivity of the matrix were monitored for 900 days. Results show that the cementation of ion-exchange resins seems not to pose special risks regarding the corrosion of the steel drums.&nbsp;                                                                       ","palabras_clave":"waste management|ion-exchange resin|cementation |steel corrosion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51488,"idioma_id":4,"titulo":"Study of the immature stages of two species of the biting midge genus Culicoides (Diptera: Ceratopogonidae)","resumen":"Pupa of Culicoides insignis Lutz, 1913, and larva and pupa of Culicoides bambusicola Lutz, 1913 are fully redescribed. They are illustrated and  photographed using binocular microscope, phase-contrast stereoscope at oil immersion  and scanning electronic microscopy, from material collected in Corrientes and  Misiones provinces, Argentina.  Palatum, maxilla and hypopharynx of the fourth instar larva of C. bambusicola as well as the cephalothoracic sensilla and  sensillar pattern of the first and fourth abdominal segment of the pupa are  described for both species. The immatures of C. bambusicola are compared with Culicoides debilipalpis Lutz, 1913. Pupa  of C. insignis is compared with the following  related congeners: C. maruim Lutz, 1913, C.  charruus Spinelli &amp; Martinez,  1992, and C. annetae Spinelli &amp; Borkent, 2004.","palabras_clave":"Ceratopogonidae|Culicoides|larva|pupa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51515,"idioma_id":4,"titulo":"Microbial transformation of S-(-)-Perillyl Alcohol by the filamentous fungi Fusarium verticillioides","resumen":"S-(-)-Perillyl alcohol (1) was transformed by Fusarium verticilloides over 7 days at 25°C to S-(-)-Perillyl aldehyde (2). Its structure was established by NMR spectroscopic and mass spectral studies. Total antioxidant potential and the radical-scavenging activity were determined by phosphomolybdenum and ABTS?+ assays, respectively. The results demonstrated that the oxidation of the primary alcohol of 1 had no effect on antioxidant activity when compared to starting material and a reference compound. Obtaining derivatives of 1, is an important goal of research in microbial biotechnology and medicinal chemistry.","palabras_clave":"Transformación Microbiana|Biotecnologia|Quimica Medica|Actividad Antioxidante"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51596,"idioma_id":4,"titulo":"EFFECT OF DIFFERENT PROTEIN SOURCES ON THE ANTIOXIDANT DEFENSE SYSTEM LUNG RAT EXPOSED TO CADMIUM","resumen":"Cadmium (Cd) is a toxic element and an important environmental contaminant. We studied its effecton the antioxidant defense system of rat lung underdifferent diets. Four lots of female Wistar rats were used: 2lots received casein (Cas) and 2 lots soybean (Soy) as proteinsource. Within each group, 1 lot received regular water (control-Co)and the other, 15 ppm of Cd in the drinking water for 60 days.Homogenates were made and TBAR?S and CAT activity weredetermined. Total RNA was isolated with Trizol and cDNA wasobtained. Superoxide Dismutase (SOD), Nuclearfactor-erythroid 2 related factor 2 (nrf-2), p47phox, Glutathion peroxidase(GPx) and NADPH Oxidase (NOX-2) were determined by PCR.S28 was the control. TBAR?S showed a significantincrease in both Cd groups and CAT activity showed a not significantincrease in the Soy groups (higher in the Cd group). Nrf-2(p&lt;0.01), p47phox (p&lt;0.05), SOD (p&lt;0.05) and GPx (p&lt;0.05)showed significant increase in the Soy Cd groups, even though NOXshowed a decrease in both Soy groups (p&lt;0.05). Weconclude that Cd induces several changes on the antioxidantdefense system and that Soy would not protect the lung against Cdintoxication, at least at this level&nbsp;","palabras_clave":"CADMIO|OXIDATIVE STRESS|LUNG"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51901,"idioma_id":5,"titulo":"Formación de las elites. Investigaciones y debates en Argentina, Brasil y Francia","resumen":"En este libro, Sandra Ziegler y Victoria Gessaghi, ambas investigadoras del Área de Educación de FLACSO- compilan un total de 13 artículos, no publicados con anterioridad, de cientistas sociales de Argentina, Brasil y Francia, en los que se aborda el tópico de la constitución de las elites, indagando en la construcción social de los privilegios. La obra constituye una propuesta original en tanto dicho tema, ampliamente estudiado en los países centrales, presenta en Argentina un desarrollo más errático, puesto que la sociología local se abocó mayormente al estudio de las clases medias y populares.","palabras_clave":"Educación|Desigualdad educativa|Formación de élites"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":51985,"idioma_id":4,"titulo":"Representation Equivalent Bieberbach Groups and Strongly Isospectral Flat Manifolds","resumen":"Let $\\Gamma_1$ and $\\Gamma_2$ be Bieberbach groups contained in the full isometry group $G$ of $\\mathhb{R^n}$. We prove that if the compact flat manifolds $\\Gamma_1\\backslash \\mathbb{R}^n$ and $\\Gamma_2\\backslash \\mathbb{R}^n$ are strongly isospectral then the Bieberbach groups $\\Gamma_1$ and $\\Gamma_2$ are representation equivalent, that is, the right regular representations $L^2(\\Gamma_1\\backshalsh G)$ and $L^2(\\Gamma_2\\backshalsh G)$ are unitarily equivalent.","palabras_clave":"representation equivalent|strongly isospectrality|compact flat manifolds"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52029,"idioma_id":4,"titulo":"Effect of prolactin acting on thr coeliac ganglion via the non-lactacting rat.","resumen":"Whether prolactin (PRL) has a luteotrophic or luteolytic effect in the rat ovary depends on the nature of the corpora lutea present in the ovaries and the hormonal environment to which they are exposed. The aim was to investigate the effect of PRL acting on the coeliac ganglion (CG) on the function of the corpora lutea on day 4 postpartum under either lactating or non-lactating conditions, using the CG-superior ovarian nerve-ovary system. The ovarian release of progesterone (P), estradiol, PGF2&#945;, and nitrites was assessed in the ovarian compartment at different incubation times. Luteal mRNA expression of 3&#946;-HSD, 20&#945;-HSD, aromatase, PGF2&#945; receptor, iNOS, Bcl-2, Bax, Fas and FasL was analysed in the corpus luteum of pregnancy at the end of the experiments. Comparative analysis of control groups showed that the ovarian release of P, nitrites, and PGF2&#945;, the expression of PGF2&#945; receptor, and the Bcl-2\/Bax ratio were lower in non-lactating rats, with increased release of estradiol, and higher expression of aromatase, Fas and FasL, demonstrating the higher luteal functionality in ovaries of lactating animals. PRL added to the CG compartment increased the ovarian release of P, estradiol, nitrites and PGF2&#945;, and decreased the Bcl-2\/Bax ratio in non-lactating rats; yet, with the exception of a reduction in the release of nitrites, such parameters were not modified in lactating animals. Together, these data suggest that the CG is able to respond to the effect of PRL and, via a neural pathway, fine-tune the physiology of the ovary under different hormonal conditions.","palabras_clave":"PROLACTINA|COELIAC|GANGLION|RAT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52071,"idioma_id":5,"titulo":"Los hijos 'puente'","resumen":"La propuesta que presentamos se basa en dos ejes principales: la construcción identitaria en hijos de inmigrantes y su rol como mediadores en el proceso de socialización de los padres. El artículo está basado en un trabajo de campo que estamos realizando en la actualidad, en un pueblo rural de la provincia de Barcelona (Moià) que ha tenido un crecimiento poblacional importante, debido a las migraciones tanto internas como externas. Por ello resulta interesante estudiar la relación entre estos colectivos y la población tradicional, y en este marco los intentos de adecuación de la escuela a las nuevas circunstancias.   Para el estudio hemos tomado a familias inmigrantes que están viviendo en Cataluña hace más de cuatro años y han tenido hijos aquí o los han traído de muy pequeños (0-2 años). Pensamos en niños de parvulario y ciclo inicial. El análisis está basado en entrevistas en profundidad a padres y maestros del colegio público, además de la observación participante en distintos ámbitos clave (festividades, cotidianeidad escolar, acciones institucionales).     Del estudio realizado se advierte el carácter de ?puente? de los niños en los distintos aspectos como la incorporación de la comida, las tradiciones, la lengua y las prácticas de socialización. Se advierte el protagonismo de la escuela y de una educación intercultural tanto para el desarrollo de esta mediación como en la construcción identitaria de los infantes. Desde la perspectiva de la identidad como un proceso y no como una esencia, hemos observado que si el niño se siente integrado, reconocido y respetado, esta compleja construcción se vivencia de manera mucho menos conflictiva en todo el grupo familiar. No obstante, no se puede dejar a la escuela toda la responsabilidad, entre los adultos se observa una mayor dificultad para la integración, más que discriminación lo que suele haber es indiferencia, de ahí la importancia de acciones que fomenten la interacción.","palabras_clave":"identidad|diversidad|integración|escuela"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52076,"idioma_id":4,"titulo":"Phylogenetic relationships of the South American ground beetle subgenus Chilioperyphus Jeannel (Coleoptera: Carabidae: Trechinae: Bembidiini: Bembidion Latreille).","resumen":"The boundaries and relationships of the ground beetle group Chilioperyphus Jeannel (a subgenus of the cosmopolitan genus Bembidion Latreille) are examined using DNA and morphological data. DNA sequence data from seven genes (six nuclear and one mitochondrial) indicates that Chilioperyphus (as newly defined) is monophyletic, and is related to the subgenera Antiperyphanes Jeannel and Plocamoperyphus Jeannel, within the South American Antiperyphanes Complex. Chilioperyphus includes two described species, B. mendocinum Jensen-Haarup and B. orregoi Germain. Bembidion cassinense Roig-Juñent and Gianuca as well as Bembidion cuyanum Roig-Juñent and Scheibler, formerly placed in subgenus Chilioperyphus, are transferred to subgenus Antiperyphanes. Bembidion cuyanum is considered a junior synonym of B. hirtipes Jeannel. The male genitalia of Chilioperyphus is unique in having a very long flagellum that is folded twice, allowing it to fit much of its length within the walls of the median lobe. However, the brush sclerite and basal part of the flagellum are not contained within the median lobe, as they extend anterior to its base","palabras_clave":"South America|Bembidion|Phylogeny"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52327,"idioma_id":4,"titulo":"Autosomal Dominant Polycystic Kidney Disease: Prevalence of Renal Neoplasias in Surgical Kidney Specimens.","resumen":"Background: The role of autosomal dominant polycystic kidney disease (ADPKD) as a risk factor for renal cell carcinoma (RCC) is still under discussion. Data on prevalence of RCC in ADPKD are limited, especially on a large population scale. The aim of this study was to analyze the prevalence of RCC in ADPKD kidneys and characterize the clinical features of this coincidence. Methods: Based on our histopathological registry for ADPKD and the Else Kröner-Fresenius Registry, we retrospectively reviewed malignant and benign renal lesions in patients with ADPKD who had undergone renal surgery from 1988 to 2011. Results: 240 ADPKD patients underwent 301 renal surgeries. Mean age at surgery was 54 years. Overall, 16 malignant and 11 benign lesions were analyzed in 301 kidneys (5.3%; 3.7%), meaning that 12\/240 (5%; 1:20) patients presented with malignant renal lesions. 66.7% (8\/12) of these patients had undergone dialysis prior to surgery. We found 10\/16 (63%) papillary RCC, 5\/16 (31%) clear cell RCC, and 1\/16 (6%) papillary noninvasive urothelial cancer. Regarding all renal lesions, 6\/17 (35.3%) patients had more than one histological finding in their kidneys. In 2 cases, metachronous metastases were removed. Mean follow-up was 66.7 months. Conclusion: Kidney-related prevalence of RCC in ADPKD kidneys was surprisingly high. Whether or not this is due to chronic dialysis or due to the underlying disease is still speculative. Like other cystic renal diseases with an increased risk for RCC, the attending physician should be aware of the malignant potential of ADPKD, especially with concomitant dialysis. ","palabras_clave":"ADPKD|Neoplasias|surgical samples"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52407,"idioma_id":4,"titulo":"BEL beta-Trefoil. A novel lectin with antitumoral properties in king bolete (Boletus edulis) mushrooms","resumen":"A novel lectin was purified from the fruiting  bodies of king bolete mushrooms Boletus edulis, also called porcino,  cep or penny bun). The lectin was structurally characterized: its amino acid  sequence and three dimensional structure were determined. The new protein is a  homodimer and each protomer folds as beta-trefoil domain and therefore we  propose the name BEL beta-trefoil to distinguish it from the other lectin that  has been described in these mushrooms: BEL. The lectin has potent  anti-proliferative effects on human cancer cells which confers to it an  interesting therapeutic potential as an antineoplastic agent. Several crystal  forms of the apoprotein and of complexes with different carbohydrates were  studied by X-ray diffraction. The structure of the apoprotein was solved at  1.12 Å resolution. The interaction of the lectin with lactose, galactose,  N-acetylgalactosamine and the T-antigen disaccharide, Galbeta1-3GalNAc, was  examined in detail. All the three potential binding sites present in the  beta-trefoil fold are occupied in at least one crystal form and are described  in detail in this paper. No important conformational changes are observed in  the lectin when comparing its co-crystals with carbohydrates with those of the  ligand free protein.","palabras_clave":"BOLETUS EDULIS|LECTIN|BETA TREFOIL|CRYSTAL STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52445,"idioma_id":4,"titulo":"Insights from mean lake-level observations and reduced gravity data","resumen":"This paper evaluates a combined geoid model in the area of Fagnano Lake located in Tierra del Fuego, Argentina. The model includes GPS\/levelling points, gravity data and GPS buoy observations on the lake. The GPS buoy information allowed to determine a mean lake level (MLL) surface which was used to extend the geoid model to an area with restricted access by land (Del Cogliano et al. 2007). An approach to optimize the selection and distribution of the MLL data is developed in order to use them as input in the Equivalent Source Technique, and to combine them with different types of observations.Furthermore, the global geopotential model EGM2008 is validated in the remote lake area. This model does not include observed gravity data in this region. Its behaviour is compared to the results of our observations along the levelling lines. Differences of several decimetres are found when EGM2008 geoid undulations are compared to geoid undulations derived from observations in this region. In the areas where EGM2008 relies on real gravimetric observations, differences between model and observations are only of a few centimetres.The comparison of the EGM2008 model with our observations reveals the effect of missing or not representative gravity information may have on the estimation of geoid undulations in high mountain regions. The data on which EGM2008 is based in the Fagnano Lake area does not result from gravimetric observations on land. Therefore, we consider them as not representative and we show that their effect can be significant in such areas.","palabras_clave":"GEOID|MEAN LAKE LEVEL|FAGNANO LAKE|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52651,"idioma_id":5,"titulo":"Argentina, dos contracciones económicas durante los primeros gobiernos peronistas.","resumen":"        El presente trabajo pretende  indagar sobre las causas de los rezagos en el bienestar económico argentino con  respecto al crecimiento de las economías de otros países, traducidos en dos  caídas del producto bruto interno, ocurridos en la década 1946-1955 durante los  primeros dos gobiernos peronistas. Las dos contracciones de la economía acontecidas  se identifican específicamente en los años 1949 y 1952, manteniendo una  estrecha relación que explica una devaluación de la moneda en primera instancia  y la formalización de un plan de ajuste en el segundo año de análisis. Ambos  sucesos han generando diversas interpretaciones sobre las causas originarias del  escenario socioeconómico acontecido. Este estudio se remite al análisis de los  factores explicativos más relevantes ?a juicio del autor- referidos  específicamente &nbsp;a la puja en el comercio  exterior con EEUU, los términos de intercambio, la política de ingresos, la  política fiscal y el comportamiento de la deuda pública.  ","palabras_clave":"PERONISMO|INDUSTRIA|CRISIS|ECONOMIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52656,"idioma_id":5,"titulo":"Desafíos actuales para prácticas profesionales y cotidianas: ¿nuestra relación con el ambiente tiene sentido de futuro?","resumen":"    RESUMEN    El&nbsp;&nbsp; trabajo desarrolla &nbsp;acciones  de indagación de las autoras, en el Proyecto de Investigación Construcción del  conocimiento profesional de psicólogos y profesores de Psicología en sistemas  de actividad, dirigido por&nbsp; Mgter. Erausquin,&nbsp; siendo Mgter Funes  Molineri Investigadora, autora de la Tesis Aprendizajes vinculados a prácticas  y estrategias en abordajes interdisciplinarios de equipos de salud que se  ocupan de la población escolar en el ámbito público,&nbsp; dirigida por Dr.  Rovere y aprobada en 2012, en Facultad de Psicología&nbsp; UBA. El  objetivo&nbsp; ahora es indagar&nbsp; representaciones del ambiente&nbsp; en  los trabajadores de salud y educación y en los vecinos de diversas localidades  y las relaciones&nbsp; entre sus creencias y conceptualizaciones y sus  prácticas profesionales y&nbsp; cotidianas. Se analizarán regulaciones y  normativas que enmarcan&nbsp; el desempeño de los equipos de Salud y de la  comunidad&nbsp; y&nbsp; los cambios de&nbsp; conceptualizaciones y actitudes  que pueden haber ocurrido en nuestro contexto.&nbsp;El propósito es producir  &nbsp;conocimientos sobre los sentidos que adquieren el tema ambiental y las  prácticas realizadas;&nbsp; las estrategias instrumentadas por los trabajadores  de salud&nbsp; y educación y por la comunidad, o&nbsp; la ausencia de las  mismas. &nbsp;Se administrará a agentes de salud y educación un Cuestionario  para Profesionales&nbsp; sobre Situaciones-Problema de su Práctica Profesionalen la relación entre los Sujetos&nbsp; y el Ambiente .El análisis de  datos se realizará con la Matriz Multidimensional de Profesionalización en  Sistemas de Actividad&nbsp; (Erausquin, Basualdo), utilizada en la línea de  indagación que incluye al Proyecto UBACYT 2012-2015 y se ampliará con Entrevistas  en Profundidad. Los instrumentos de recolección y análisis de datos pueden  convertirse en artefactos mediadores&nbsp; para el  desarrollo&nbsp;&nbsp; de una metodología de investigación acción  participativa.   ","palabras_clave":"salud|educación|ambiente|prácticas|conceptualizaciones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52667,"idioma_id":5,"titulo":"PATRIMONIO ARQUEOLÓGICO DE LA CUENCA DEL RÍO LIMAY.  AGENTES Y PROCESOS QUE LO DESTRUYEN","resumen":"ResumenDesde hace varias décadas el área de la cuenca del río Limay (Provincias de Neuquén y Río Negro), se vio afectada por la realización de numerosas obras y actividades económicas que inciden en las poblaciones que allí viven y trabajan. Esas obras también afectan los sitios arqueológicos en estudio en forma parcial o total; asimismo los coleccionistas y huaqueros contribuyen a destruir esos sitios. Entre los más afectados se encuentran los de arte rupestre. La destrucción y el saqueo complican no sólo el estudio arqueológico sino también la integridad de las identidades originarias. Este trabajo se refiere a estas cuestiones, propone una puesta en valor del patrimonio y algunas alternativas para evitar la incidencia negativa de las obras y el saqueo.Palabras clave: Cuenca del río Limay; sitios arqueológicos; patrimonio afectado; obras y saqueos","palabras_clave":"Estudios de impacto|Sitios arqueológicos|Grandes obras|Saqueos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52754,"idioma_id":5,"titulo":"Introducción al dossier: Historias de la república. Variaciones sobre el orden político en la Argentina del siglo XIX","resumen":"Introducción al dossier coordinado por Hilda Sabato que incluye seis artículos escritos por un total de once investigadores, sobre temas relacionados con el título del mismo: Historias de la república. Variaciones sobre el orden político en la Argentina del siglo XIX.","palabras_clave":"REPÚBLICA|SIGLO XIX|ORDEN POLÍTICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52775,"idioma_id":5,"titulo":"A falta de épica  buenas son las historias.  El ?héroe? en la narrativa latinoamericana actual.","resumen":"El   presente trabajo pretende demostrar algunas variaciones que  ha   experimentado el personaje en la novela hispanoamericana, especialmente    desde los años 1970 a la actualidad. Coincidimos con los  tratadistas   en el hecho de que la épica es inconcebible no solamente porque el   género  hace mucho tiempo que está agotado. Entre la heroicidad de la   novela del boom y  la galería de personajes de la narrativa más reciente   se interponen gravísimos  episodios de la vida política   latinoamericana,   como las dictaduras de los años 1980 y las guerras  civiles   centroamericanas. Sin que existan relaciones directas e inmediatas hemos    sostenido que la declinación del héroe es posible asociarla con la   declinación  de las utopías y los grandes relatos.","palabras_clave":"NOVELA LATINOAMERICANA|HEROE|DERROTA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52873,"idioma_id":5,"titulo":"¿Clientelismo político, inscripción territorial, para-burocracia? Reflexiones sobre la sociabilidad en un barrio informal.","resumen":"Partiendo del caso del barrio informal Los Pinos, en el siguiente trabajo buscaremos discutir la noción de ?clientelismo político? para explicar algunos fenómenos de sociabilidad entre sus residentes. Consideramos que esta categoría presenta interpretaciones que se prestan a lecturas superficiales y claudica la comprensión de formas de sociabilidad más complejas.Este artículo buscará problematizar esa literatura a partir de dos momentos. En primer lugar, se hará una breve referencia a la toma de la tierra para dar cuenta de una historia de lucha colectiva en pos de la construcción de un barrio. En segundo lugar, presentaremos una situación social en la que el Gobierno de la Ciudad, a partir de la Unidad de Gestión e Intervención Social (UGIS), ?baja? materiales luego de una fuerte tormenta que tuvo lugar el 11 de abril de 2012 en la que las viviendas se vieron afectadas.En la última sección intentaremos cuestionar la aplicación del concepto de clientelismopolítico para este caso. Esta noción ha monopolizado la agenda de investigaciones delos últimos tiempos para considerar las formas de participación política de los sectores populares. Para ese fin, retomaremos a Auyero (2001a) quien presenta una aproximación al clientelismo político para ponerlo en tensión a partir del trabajo de Merklen (2010).Habiendo pasado por estas discusiones intentaremos dar cuenta de una nueva lectura de la entrega de materiales a partir de la categoría de para-burocracia, recientemente acuñada por Vitale (2012) para considerar la relación entre las estructuras organizativasen villas y los aparatos burocráticos.","palabras_clave":"Sociabilidad|Asentamientos Informales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52887,"idioma_id":5,"titulo":"Capital brasileño y enajenación productiva de la gran burguesía argentina en la posconvertibilidad: un ensayo de interpretación sociológica","resumen":"El objetivo del presente trabajo remite a realizar un recorrido por la trayectoria de expansión del capital brasileño en la Argentina desde la salida de la convertibilidad, identificar sus dimensiones, formas y significantes. Para ello se propone un tratamiento dividido en dos momentos. En primer lugar una aproximación de carácter empírico-descriptiva acudiendo a un conjunto de herramentales estadísticos sobre variables representativas, a los fines de poner de manifiesto la relevancia cognitiva del fenómeno.   En segundo término, se esbozará un intento de aproximación analítica sobre el carácter y significado del proceso de ?brasileñización? de la economía argentina a partir de una propuesta del saber económico con foco en los determinantes históricos y sociales del proceso, perspectiva que se propone superadora del individualismo metodológico neoclásico del análisis económico. Por último se realizará una recapitulación sintética   de los principales aspectos del trabajo y se esbozaran una serie de conclusiones.","palabras_clave":"CAPITAL|POSCONVERTIBILIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":52995,"idioma_id":5,"titulo":"oikos bio politica","resumen":"El siguiente artículo propone discutir si se puede establecer un camino intelectual hacia una biopolítica de la naturaleza o del entorno. Dicho menos vulgarmente, una  política del gobierno de la vida más allá de lo humano.  Pensar en una biopolítica, cruzando el ámbito de la vida pública humana, avanzando sobre la esfera del ambiente. Nos referimos a una bio-óikos-política, preocupación que  puede extraerse de los mismos discursos ensayísticos de Michel Foucault, especialmente, en su lectura de la ilustración y de los discursos. Por otro lado, la lectura de la exceptio de la nuda vida  de Giorgio Agamben desde su propuesta de ?excepción?.  A partir de ellos se propone la noción de bíopolitica, como campo abierto, noción precautoria  y constante, también como nuevo paradigma de gubermentalidad.","palabras_clave":"CASA|AMBIENTE|BIOPOLITICA|FOUCAULT RORTY AGAMBEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53070,"idioma_id":5,"titulo":"CAPACIDADES  DINÁMICAS: LÍNEAS PROMISORIAS Y DESAFÍOS DE INVESTIGACIÓN","resumen":"El marco de capacidades dinámicas ha recibido mucha atención en los campos de la estrategia, la administración y la economía porque ayuda a entender cómo las empresas enfrentan los cambios en el entorno, un tema central en el contexto de mercados abiertos y economías globalizadas. No obstante, hay intensas controversias que se deben identificar y resolver para poder desarrollarlo como cuerpo teórico consolidado. Este artículo revisa las principales áreas de debate y sugiere líneas promisorias de investigación con base en un grupo de artículos relevantes publicados desde 1997, seleccionados de acuerdo con el número de citas.","palabras_clave":"CAPACIDADES DINÁMICAS|GESTIÓN ESTRATÉGICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53073,"idioma_id":5,"titulo":"INTRODUCCIÓN AL ESTUDIO DE LA CIENCIA Y LA TECNOLOGÍA","resumen":"El propósito del trabajo es realizar una revisión bibliográfica que dé cuenta e integre los principales conceptos, corrientes filosóficas y  metodológicas y los principales procesos científicos determinantes de los cambios en el desarrollo del conocimiento, en particular en el campo de las ciencias sociales, a fin de realizar un análisis crítico de los trabajos académicos en campos diversos de la ciencia y la tecnología, que permita plantear también las principales implicancias prácticas de los mismos, en términos de la formulación de políticas científicas, proyectos de investigación y conductas del investigador.","palabras_clave":"Ciencia| Tecnología |Metodología de la Ciencia| Sociología de la Ciencia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53126,"idioma_id":5,"titulo":"DERECHOS INDÍGENAS EN ARGENTINA. Territorios y propiedad comunitaria","resumen":"Neste artigo vamos examinar a regulamentação e implementação das leis em relação à terra\/território e propriedade da comunidade indígena, a partir das discussões geradas pela apresentação do projecto de Reforma, Actualização e Unificação dos Códigos Civil e Comercial da Argentina. Para este fim, vamos discutir os itens deste projecto que referem a os povos indígenas, as propostas de reformulação feitas por diferentes organizações e povos indígenas, e os argumentos que avançam contra a incorporação do tratamento de propriedade da comunitaria indígena.Mi lugar en este trabajo como primera autora obedece al hecho de haber realizado: 1) la sistematización de datos obtenidos en el contexto de la Audiencia Pública llevada a cabo en La Plata el día 13 de septiembre de 2012. 2) el aporte particular referido a la elaboración teórica en torno a los derechos territoriales de los pueblos indígenas","palabras_clave":"CODIGO CIVIL|PUEBLOS INDÍGENAS|IDENTIDADES ÉTNICAS|TERRITORIOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53160,"idioma_id":5,"titulo":"Las movilizaciones sociales en los `90 y la criminalización de la prtesta social en Argeitna: su relación con la estatización de YPF en el 2012","resumen":"La relación existente entre la medida adoptada por el Ejecutivo de la República Argentina en abril del 2012, mediante la presentación al Congreso de la Nación del proyecto de ley de estatización de Yacimientos Petrolíferos Fiscales, con el texto a continuación que analiza la protesta social y la acción del sistema judicial, está dada por la relación histórica que tiene las medidas de protesta social de cortes de ruta con las medidas políticas económicas que se dieron en el marco del achicamiento del Estado durante ?la era menemista? de los ´90, es decir, la estrecha relación entre la protesta social con la soberanía sobre los recursos naturales del país. En otro trabajo (Manzi, 2012) hemos analizado el concepto de ideología cuando los cortes de ruta y de calle salen emergen en la vida pública interrumpiendo el tránsito normal de la población, o interrumpiendo el goce de ciertos derechos. Afirmamos que las resistencias de las movilizaciones argentinas encontraron su saber, no en la visibilidad de la calle y en la superposición de derechos, sino en la geografía de la economía del libre tránsito En este sentido, es un resarcimiento simbólico y material a la sociedad en términos de soberanía.","palabras_clave":"Protesta social|Criminalización|Disciplinamiento"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53166,"idioma_id":5,"titulo":"EL ANÁLISIS DEL DISCURSO Y SUS APORTES A LAS LENGUAS EXTRANJERAS: ANÁLISIS DE LA SITUACIÓN Y ESTUDIO DE APLICACIONES","resumen":"El presente trabajo tiene como propósito principal analizar el estado del arte del AD en relación con las lenguas extranjeras (LE), segundas lenguas (L2) y la enseñanza de Español como lengua extranjera (ELE) con énfasis en los aportes que diversas corrientes teóricas han brindado a la teoría lingüística y, en consecuencia, a aplicaciones didácticas. Asimismo, nos proponemos ilustrar el aporte por medio del relato de experiencias didácticas de base analítico discursiva llevadas a cabo en diversos niveles de formación en enseñanza de Inglés en la Universidad Nacional de San Luis. En el plano teórico conceptual, las experiencias presentadas se basan en los principios propuestos por la Lingüística Sistémico Funcional (LSF) postulados por Halliday (1978), Halliday y Hasan (1976, 1985) y por Halliday y Matthiessen (2004).","palabras_clave":"Análisis del Discurso|Lenguas Extranjeras|Lectura|Educación Superior"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53192,"idioma_id":5,"titulo":"Los valores jurídicos y la bioética. Hacia una nueva relación entre moral y derecho","resumen":"En este trabajo se trata de demostrar que la correcta solución de los dilemas bioéticos debe estar acompañada de una teoría de los valores objetivista. Se efectúan, asimismo, apreciaciones referidas al principio de autonomía kantiano y como éste se encuentra mal interpretado por muchos autores modernos","palabras_clave":"BIOÉTICA|MORAL|DERECHO|VALORES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53195,"idioma_id":5,"titulo":"El concepto de derecho en Ronald Dworkin","resumen":"El artículo realiza un panorama respecto de la principal tesis de Dworkin: el derecho como conjunción de normas y principios. También se ahonda en los principios interpretativos más relevantes para este autor.","palabras_clave":"PRINCIPIOS JURIDICOS|POSITIVISMO JURIDICO|CASOS DIFICILES|RONALD DWORKIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53198,"idioma_id":5,"titulo":"¿Cómo debe interpretarse el art. 8º de la ley 24.374?","resumen":"Estudio de la naturaleza jurídica del plazo previsto en el artículo 8 de la ley 24.374","palabras_clave":"PRESCRIPCIÓN ADQUISITIVA|PRESCRIPCIÓN TABULAR|DERECHOS REALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53202,"idioma_id":5,"titulo":"\u201CJustificación de la categoría de las obligaciones naturales. Bases para su adecuada regulación","resumen":"Presentación Institucional dela Facultad de Derecho de la Pontificia Universidad Católica Argentina SantaMaría de los Buenos Aires en las XXIV Jornadas Nacionales de Derecho Civil","palabras_clave":"OBLIGACIÓN NATURAL|DERECHO NATURAL|DERECHO POSITIVO|FILOSOFIA DEL DERECHO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53205,"idioma_id":5,"titulo":"Aplicación del artículo 2618 del Código Civil a la luz de principios y normas de derecho ambiental","resumen":"Análisis del artículo 2618 del Código Civil que regla las relaciones de convivencia a la luz de los principios del derecho ambiental, como el de prevención y precaución.","palabras_clave":"LÍMITES AL DOMINIO|RELACIONES DE VECINDAD|DERECHO AMBIENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53219,"idioma_id":4,"titulo":"Liver glycerol permeability and Aquaporin-9 are dysregulated in a murine model of Non-Alcoholic Fatty Liver Disease","resumen":"One form of liver steatosis, namely Non-Alcoholic Fatty Liver Disease (NAFLD), is a worrisome health problem worldwidecharacterized by intrahepatic triacylglycerol (TG) overaccumulation. NAFLD is a common feature of metabolic syndromebeing often associated with obesity, dyslipidemia and diabetes and mostly closely linked to insulin resistance. Themechanism of NAFLD pathogenesis is object of intense investigation especially regarding complex systems ultimatelyresulting in excessive TG deposition in hepatocytes. However, scarce is the attention about the relevance of hepatic importof glycerol, the other primary source (as glycerol-3-phosphate) of increased TG in hepatocytes. Obese leptin-deficient (ob\/ob) mice, an animal model of NAFLD, were used to evaluate the functional involvement of Aquaporin-9 (AQP9), the majorpathway of liver glycerol entry, in hepatosteatosis. By RT-PCR and qPCR, the level of Aqp9 mRNA in the liver of starved obesemice was comparable with the corresponding control lean littermates. By immunoblotting, the AQP9 protein at thehepatocyte sinusoidal plasma membrane of obese mice was markedly lower (33%) than lean mice, a finding fully confirmedby immunohistochemistry. By stopped-flow light scattering, the liver glycerol permeability of ob\/ob mice was significantlylower (53%) than lean mice, a finding consistent with both the observed down-regulation of AQP9 protein and increasedlevel of plasma glycerol characterizing obese mice. In summary, our results suggest implication of AQP9 in liver steatosis.The reduction of hepatocyte AQP9 and, consequently, glycerol permeability might be a defensive mechanism to counteractfurther fat infiltration in liver parenchyma.","palabras_clave":"Aquaporin-9|glycerol|NAFLD|liver"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53221,"idioma_id":4,"titulo":"Mitochondrial aquaporin-8: a functional peroxiporin?","resumen":".","palabras_clave":"aquaporin-8|mitochondria|hydrogen peroxide|hepatocyte"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53237,"idioma_id":5,"titulo":"Cuatro ases: críticos y sociólogos en Brasil y en Argentina","resumen":"El estudio aislado de trayectorias intelectuales suele ser víctima de un error que Pierre Bourdieu ha denominado ?ilusión biográfica?. La estrategia analítica de comparar itinerarios individuales inscriptos en contextos aproximables permite eventualmente una aprehensión más atenta a los condicionantes y a las circunstancias sociales incorporadas en los mismos. En tal dirección examinaremos en este artículo la trayectoria de cuatro personajes ?Florestan Fernandes y Gino Germani, Antonio Candido y Adolfo Prieto? que fueron protagonistas de la modernización de la sociología y la crítica literaria respectivamente en Brasil y Argentina entre las décadas de 1940","palabras_clave":"TRAYECTORIAS|BIOGRAFÍA|SOCIOLOGIA|CRÍTICA LITERARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53387,"idioma_id":5,"titulo":"La incorporación de la gobernanza a la agenda Latinoamericana","resumen":"Este artículo se aboca a un breve análisis relativo a los orígenes y debates conceptuales relativos a la gobernanza y su aplicación al caso de América Latina. En este último espacio no ha tenido la mejor de las acogidas puesto que otros términos como el de gobernabilidad o el de gobierno, han respondido a una tradición en el análisis político regional como producto de un pasado reciente turbulento en lo relativo a las alteraciones al sistema democrático.   Artículo del Grupo 1 en prensa. Al momento de la presentación no se obtuvo el certificado que acredita que el artículo se encuentra en prensa, mediando sólo un e mail de confirmación.","palabras_clave":"GOBERNANZA|AMÉRICA LATINA|GOBIERNO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53404,"idioma_id":4,"titulo":"THE RIO DE LA PLATA ESTUARY RESPONSE TO WIND VARIABILITY IN SYNOPTIC TIME SCALE: SALINITY FIELDS AND BREAKDOWN AND RECONSTRUCTION OF THE SALT WEDGE STRUCTURE","resumen":"The Río de la Plata estuary response to wind variability in synoptic time scale is studiedwith the Estuary, Coastal and Ocean Model forced by tides, runoff and idealized winds. AncillaryCTD data from synoptic oceanographic cruises are used to validate the conclusions extractedfrom the simulations. Results show that the estuary response to wind variability can be explainedin terms of four characteristic patterns associated with winds that blow with a dominantcomponent either along or across the estuary axis. Cross-estuary winds from the southwest(northeast) produce a northward displacement (southwestwards retraction) of the surface salinityfront towards (from) the Uruguayan coast and cause downwelling (upwelling) betweenMontevideo and Punta del Este. Along-estuary winds with a downstream (upstream) componentproduce a net outflow (inflow) of continental surface waters and inflow (outflow) of bottom shelfwaters, resulting in an intensification (weakening) of the vertical structure. Stratification can becompletely destroyed by strong or persistent southeasterly winds, which are not frequent in theregion, and it completely recovers in a relatively short period of time (10 - 15 days) after windrelaxation. Consequently the salt wedge structure is present in the Río de la Plata during most ofthe year. This implies that significant vertical mixing events producing exchanges of water andproperties between the surface and bottom waters occur only a few times along the year. As aresult, the Río de la Plata shows strong retentive features favoring biota by retaining eggs andlarvae, but also favoring pollutant accumulation.","palabras_clave":"STRATIFICATION|WIND FORCING|VERTICAL STRUCTURE|RIO DE LA PLATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53440,"idioma_id":5,"titulo":"El futuro y el tiempo histórico en la filosofía de la historia de Hegel","resumen":"In this paper we analyze the role that the  future plays in the Hegelian philosophy of history. Mind requires time to reach  a mediate consciousness of itself, since it gains its reality through the  process of the universal history. There is an evolution in history because Mind  is the absolute possibility. But Mind is always present so it seems that no  future is needed for its development. The future can be understood as a  dimension of the historical time or as a logical condition derived from the  teleological structure of Mind. In the latter, the future appears as a  nonhistorical time because in the universal history only what is needed  happens, that is, the fulfillment of the ideal. What we will try to show is  that the very Hegelian model of history requires the future as historical time  in order to grasp the political nature of Mind as State.","palabras_clave":"HEGEL |FUTURO|HISTORIA|ESPIRITU"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53576,"idioma_id":4,"titulo":"A reappraisal of Neocalamites and Schizoneura (fossil Equisetales) based on material from the Triassic of East Antarctica","resumen":"Sphenophytes are a common floral element in the Triassic of Gondwana. Most sphenophyte compression fossils have been conventionally assigned to a few, presumably very widespread species of Neocalamites based on vegetative features of the stems (or pith casts) and the foliage. During recent decades, however, new reports on morphological and anatomical details of some of these fossils have cast doubt on the systematic affinities of many Gondwanan Triassic sphenophytes. Here we describe Neocalamites suberosus (Artabe & Zamuner) nov. comb. et emend. and Schizoneura africana Feistmantel emend. from several Triassic deposits in the central Transantarctic Mountains and Victoria Land, East Antarctica. The material enables a critical reevaluation of morphological and anatomical features that have been historically used to define the two genera, including leaf- base morphology, degree of leaf fusion, stem vasculature and vallecular canals, and features of the nodal diaphragm. The diagnoses of Neocalamites and Schizoneura are emended so that they more accurately reflect recent advances in our understanding of the anatomy and ontogeny of these plants.","palabras_clave":"SPHENOPHYTA|PARASCHIZONEURA|NODODENDRON|TRANSANTARTIC MOUNTAINS|VICTORIA LAND"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53581,"idioma_id":4,"titulo":"(2151) Proposal to conserve the name Marattiopsis (fossil Marattiaceae) with a conserved type","resumen":"N\/A","palabras_clave":"n\/a"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53609,"idioma_id":4,"titulo":"PLANT APHID SPECIES PRESENT IN PEPPER CROPS (Capsicum annuum L.) IN TUCUMÁN, ARGENTINA","resumen":"Pepper is a species native to Central America belonging to the Solanaceas family. There are numerous plague species that colonize it:trips, white flies, lepidoptera and plant aphids. Among aphid species in Argentina, Myzus persicae Sulzer as a key plague followed byAulacorthum solani (Kaltenbach) and Macrosiphum euphorbiae (Thomas) can be mentioned. Aphis gossyii Glover, Brevicorynebrassicae L. and Rhopalosiphum rufiabdominalis (Sasaki) are also mentioned in Chile. The action of M. persicae and M. euphorbiaewas highlighted because of their quality as vectors. The objective of this work was to determine the aphids of pepper crops in twoTucumán horticultural zones. Directed samplings in experimental fields of La Ramada (Burruyacú) and Lules were conducted. Adultswere separated and kept in 70º ethyl alcohol and in semi-permanent microscopic preparations (Martin's method, 1983). Identificationswere performed using taxonomical keys and morphological descriptions from different authors. Two species were identified:Aulacorthum solani (Kaltenbach) in both localities and Myzus persicae (Sulzer) only in Lules. It is important to know the main aphidspecies present in pepper crops because of the damage they cause and their virus transmission activity.","palabras_clave":"APHIDIDAE|Capsicum annuum L.|PLAGUE SPECIES|MYZUS PERSICAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53626,"idioma_id":5,"titulo":"Leptospirosis humana estudio para la obtención de herramientas de diagnóstico y evaluación de la utilidad del serodiagnóstico en diferentes etapas de la enfermedad","resumen":"RESUMEN La leptospirosis humana es la zoonosis más ampliamente distribuida en el mundo. Sin embargo, la información publicada sobre las características clínicas y epidemiológicas de la enfermedad en Argentina es muy escasa. Por otra parte, dado que las técnicas diagnósticas de referencia no permiten la detección temprana de casos, es necesario desarrollar y evaluar nuevas pruebas. Los Objetivos Generales de esta tesis fueron: 1. Desarrollar ELISAs IgG para detectar anticuerpos antileptospiras en muestras de suero humano; 2. Evaluar el error sistemático global y en diferentes etapas de la evolución de la enfermedad y el error aleatorio de los ELISAs desarrollados y de la macroaglutinación (TR); 3. Evaluar la presentación clínica y epidemiológica de los casos de leptospirosis e identificar factores de riesgo para la adquisición de la enfermedad. Se obtuvieron 11 antígenos extractivos mediante cultivo y lisado de cepas patrón de Leptospira interrogans y biflexa. Se realizó un mapeo de epitopes de LipL32 y LipL41 de L. interrogans serovar Copenhageni, identificándose dos regiones antigénicas (P1 y P2) en la LipL32. Se diseñaron y obtuvieron dos péptidos sintéticos y tres proteínas recombinantes. Posteriormente se desarrollaron cuatro ELISAs para la detección de anticuerpos IgG en muestras de suero sanguíneo utilizando cuatro antígenos elegidos en base a su capacidad discriminatoria: uno con antígeno extractivo (serovares Pyrogenes y Tarassovi), dos con péptidos sintéticos (P1-ELISA y P2-ELISA) y uno con el recombinante conteniendo la secuencia de ambos péptidos (REC-ELISA). El criterio de definición de casos incluyó los resultados de la Microaglutinación (MAT) y los antecedentes clínicos y de laboratorio general. Los tiempos de evolución se dividieron en tres etapas: 1 (&lt;10 días), 2 (de 10 a 25 días) y 3 (&gt;25 días). El ELISA desarrollado con antígeno extractivo permitió en la etapa 1 mayor detección de casos que el TR, la MAT y los otros tres ELISAs, haciéndolo desde el cuarto día de evolución de la enfermedad. En la etapa 2 evidenció la mayor sensibilidad 95.6% (89,1; 98,8), especificidad 95.7% (89,2; 98,8), VPP 95,7% (89,2; 98,8), VPN 97,7% (91,9; 99,7) y AUC 0,974 (0,938; 0,992). En la etapa 3 y en la evaluación global mostró mayor AUC 0,943 (0,840; 0,988) que los otros ELISAs y una concordancia excelente entre sus resultados. Estos hallazgos indican que en el caso de seleccionar un solo antígeno, el extractivo sería la mejor opción. La baja sensibilidad del TR en la primera etapa, su baja especificidad y su variabilidad intra e inter-operador, indicarían que no es conveniente su utilización como única prueba tamiz. Las AUCs de los cuatro ELISAs fueron mayores en las etapas 2 (de 0,974 a 0,787) y 3 (de 0,943 a 0,818) debido principalmente a la baja sensibilidad en la etapa 1. En coincidencia, el meta-análisis identificó que las pruebas de ELISA presentan mayor eficacia cuando se aplican en individuos en fase convaleciente. Por lo expuesto, ningún método serológico sería capaz de resolver individual y completamente el diagnóstico en los primeros 9 días de la enfermedad. En esta primera etapa, la RT-PCR fue el método que permitió la mayor detección de casos, seguido del ELISA extractivo. Estos resultados avalan la conclusión de que un método directo, como la RT-PCR, detecta los casos de leptospirosis de forma más temprana que los métodos serológicos. Se concluye entonces en primer lugar, que RT-PCR sería el mejor método para la detección precoz de casos en muestras de hasta seis días de evolución. Luego una combinación de RT-PCR y ELISA extractivo resolvería el problema del diagnóstico en esta etapa en forma global. Finalmente si las muestras son de entre 6 y 9 días de evolución el ELISA extractivo sería la mejor opción. El serogrupo presumiblemente infectante más frecuente fue Icterohaemorrhagie seguido de Pomona, Ballum y Canicola. La mayoría de los casos ocurrieron en adultos de sexo masculino durante los meses cálidos y lluviosos, con fiebre, dolor de cabeza y mialgia. La inyección conjuntival se manifestó en el 55% de los casos confirmados y en el 43% de los No casos. Aunque el trabajo rural continúa siendo un factor de riesgo en Argentina (OR= 3,41; 1,45- 8,06), el contacto prolongado con inundaciones fue el factor más importante&nbsp; (OR= 4,49; 1,17-17,25). ","palabras_clave":"LEPTOSPIROSIS|DIAGNOSTICO|SALUD PUBLICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53665,"idioma_id":4,"titulo":"Reactive oxygen species production in the phagosome: impact on antigen presentation in dendritic cells","resumen":"Abstract  SIGNIFICANCE:  The NADPH oxidase 2 (NOX2) is known to play a major role in innate immunity for several decades. Phagocytic cells provide host defense by ingesting microbes and destroy them by different mechanisms, including the generation of reactive oxygen species (ROS) by NOX2, a process known as oxidative burst. The phagocytic pathway of dendritic cells (DCs), highly adapted to antigen processing, has been shown to display remarkable differences compared to other phagocytes. Contrary to macrophages and neutrophils, the main function of DC phagosomes is antigen presentation rather than pathogen killing or clearance of cell debris.  RECENT ADVANCES:  In the last few years, it became clear that NOX2 is also involved in the establishment of adaptive immunity. Several studies support the idea of a relationship between antigen presentation and the level of antigen degradation, the latter one being regulated by the pH and ROS within phagosomes.  CRITICAL ISSUES:  The regulation of phagosomal pH exerted by NOX2, and thereby of the efficacy of antigen cross-presentation in DCs, represents a clear illustration of how NOX2 can influence CD8(+) T lymphocyte responses. In this review, we want to put emphasis on the relationship between ROS generation and antigen processing and presentation, since there is growing evidence that the low levels of ROS generated by DCs play an important role in these processes.  FUTURE DIRECTIONS:  In the next years, it will be interesting to unravel possible mechanisms involved and to find other possible connections between NOX family members and adaptive immune responses.","palabras_clave":"IMMUNOLOGY|INFLAMMATION|NADPH OXIDASES|DENDRITIC CELLS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53734,"idioma_id":5,"titulo":"Conclusiones de las IV Jornadas de Antropología Social del Centro: La Antropología Social hoy, a diez años del inicio del nuevo siglo","resumen":"Los días 2,3 y 4 de octubre del corriente año en el predio de la   Facultad de Ciencias Sociales de Olavarría de la UNICEN, se realizaron   las IV Jornadas &nbsp;de Antropología Social del Centro.  Contando con el antecedente de las concretadas en los años 2005, 2009   y 2011, esta edición tuvo como objetivo brindar salida al potencial   investigativo de nuestros colegas y estudiantes, abrir un camino de   comunicación entre nuestras Carreras y mostrarnos en diversos espacios   del quehacer profesional y académico.  En esta oportunidad sesionaron 11 Grupos de Trabajo que&nbsp;   recepcionaron un total de 113 abstracts de autores de las siguientes   Universidades e instituciones: UBA, UNLP,&nbsp; Lanús, Quilmes, UNMdP,   Universidad Nacional del Comahue, La Pampa, Salta,&nbsp; INTA, CATUR,   Dirección Provincial de Políticas Reparatorias, de la Secretaría de   D.D.H.H de la Pcia. de Bs. As. Del extranjero: Universidades de   Colombia, Brasil, Uruguay&nbsp; y la Universidad de Padua, Italia.  Al final las exposiciones y el intercambio de experiencias de   investigación en cada grupo de trabajo, los\/as coordinadores\/as, se   dieron lugar para llegar a distintas conclusionesde acuerdo a las temáticas abordades.","palabras_clave":"Antropología"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53766,"idioma_id":4,"titulo":"Nanoberries for topical delivery of antioxidants","resumen":"With the aim of improving the antioxidant activity of polyphenols from blueberries (Vaccinium myrtillus) on skin targets after topical application, ethanolic extracts from three blueberry varieties (named Millenia, O?Neal and Blue Crisp) were loaded into ultradeformable liposomes. These nanocarriers are known to be capable of penetrating through the stratum corneum reaching its deeper layers and the viable epidermis. On the other hand, blueberries contain large amounts of polyphenols, whose antioxidant properties as tissue protectors against processes mediated by reactive oxygen species have been extensively proved. Blueberries are usually consumed as edible products, but their antioxidant compounds are poorly absorbed. The antioxidant properties of the extracts were screened before and after being loaded into ultradeformable liposomes made of soy phosphatidylcholine and sodium cholate, of nearly 100 nm in size at 0.223 extract\/lipid w\/w. The ethanolic extract-loaded ultradeformable liposomes (nano-berries) from Millenia variety retained an 85 % of the antioxidant capacity of the free extract and showed low cytotoxicity on HaCaT cells (less than 20%) at active concentration against free radicals.","palabras_clave":"BLUEBERRY|NANOCOSMETICS|ULTRADEFORMABLE|LIPOSOMES|ANTIOXIDANTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53770,"idioma_id":5,"titulo":"Sagrado\/Profano. El aspecto durkheimiano de la filosofía de la religión de William James","resumen":"El objetivo de este artículo es mostrar la existencia de un aspecto durkheimiano en la filosofía de la religión de William James, aspecto habitualmente inadvertido en las interpretaciones corrientes de su obra. Para ello mostraré como subyace a Las variedades de la experiencia religiosa la prototípica distinción durkheimiana entre lo sagrado y lo profano como rasgo esencial de la religión.","palabras_clave":"DURKHEIM|PRAGMATISMO CLASICO|RELIGION|WILLIAM JAMES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53772,"idioma_id":5,"titulo":"Caracteristicas fisico quimicas del agua de vegetacion proveniente de la molienda de Arbequina para obtencion de aceite","resumen":"A partir de la década del 90, Catamarca se convirtió en la mayor productora de aceite de oliva del país. En la industrialización de la aceituna para aceite el 80% de la materia prima molida se convierte en alperujo, un residuo constituido por la fracción sólida y el agua de vegetación de la aceituna procesada, que provoca importantes problemas ambientales. El objetivo de la presente investigación radica en determinar las características físico-químicas del agua de vegetación proveniente del alperujo de la variedad Arbequina, con la intención de lograr un mejor conocimiento los subproductos producidos en la industria olivícola, que permita su tratamiento de manera integral, rentable y ambientalmente compatible.","palabras_clave":"OLIVO|ARBEQUINA|AGUA|ALPERUJO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53774,"idioma_id":4,"titulo":"Review of Loyalty to Loyalty. Josiah Royce and the Genuine Moral Life","resumen":"Es una review de un libro de Matthew Foust sobre Royce y que fue escrita por invitación.    There are several issues that are fundamental in order to analyze the importance of this book, namely an accurate reconstruction of Royce´s moral philosophy; the integration of Royce?s morals with other philosophical traditions; and the relevance of Royce?s moral psychology to  moral philosophy, among others. In my view, however, there are two that are essential: first, the idea that loyalty to loyalty is a genuine way to make moral philosophy universalizable, entailing that this expression is not a vacuous one; second, the importance of the redemptive character of loyalty.      Foust adheres to two different Royce?s thesis: loyalty to loyalty as a moral criterion, on the one hand, and the idea of the healing\/creative task of the community, which implies that reconciliation is always possible. I think that the latter is mistaken. In this point, and taking the name of Auschwitz in a Bernsteinean way, I think that Royce?s moral philosophy can be seen as trapped within two contradictory tendencies: a clear ?pre-Auschwitz? vein, on the one hand, and relevant aspects of his moral philosophy, on the other. Regarding the first, despite his rejection of what he calls external theodicy, Royce is still looking for a reconciliation that Arendt (and Bernstein) accurately rejects. Thus, within this vein of Royce?s thought, theodicy poses a threat. Meanwhile, a second tendency refers to the significant aspects of Royce?s philosophy, particularly considering his conception of evil: first, his emphasis on the actuality of evil; second, his statement that evil is not totally intelligible; and third, his conception of the irrevocability of deeds and especially of evils.     Summing up, I think this book offers a precise reconstruction of Royce?s moral conception and therefore it is a good contribution to the growing literature on the American philosopher. My main criticism is that the book remains too attached to Royce?s view and, consequently, it fails to disclose some shortcomings that can be found in his philosophy.","palabras_clave":"CLASSICAL PRAGMATISM|EVIL|LOYALTY|MORAL PHILOSOPHY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53776,"idioma_id":4,"titulo":"Consequences of Lutheranism: James and Rorty on the Sacred","resumen":"The primary object of this article is to analyze how William James and Richard Rorty conceive the sacred. The latter explicitly follows in his  philosophy of religion a Jamesian path, which stresses the importance  of privatizing religion. I will put forward two arguments against this  interpretation: first, that James?s notion of self-surrender is incompatible with the Rortyan idea of privatization; second, that this notion of self-surrender has Lutheran roots in James?s Varieties of Religious Experience.","palabras_clave":"CLASSICAL PRAGMATISM|CONTEMPORARY PHILOSOPHY|LUTHER|RELGION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53780,"idioma_id":4,"titulo":"Royce and Bernstein on Evil","resumen":"This article has a twofold purpose: on the one hand, it aims to reconstruct Josiah Royce?s views on evil; on the other hand, it intends to examine these Roycean views within the framework of Richard Bernstein?s work, particularly his books Radical Evil: a Philosophical Investigation and The Abuse of Evil. I conclude that, despite some shortcomings regarding his notion of evil, Royce?s philosophy should be recovered as a classical voice in order to fight against the abuse of evil.","palabras_clave":"Classical Pragmatism|Contemporary Pragmatism|Josiah Royce|Richard Bernstein"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53785,"idioma_id":4,"titulo":"William James? Conception of Religion in Josiah Royce?s Mature Thought: Three Approaches","resumen":"the aims of this paper are mainly three: firstly, to reconstruct James? influence on Royce?s mature philosophy; secondly, to highlight a similarity between them: the initial distinction that both establish between religion and morality;  finally, to support two hypotheses: first, to show that Royce?s criticisms of James are mistaken, i.e., that the problem between them relates not to individual vs. social religious experiences but to justification by faith alone vs. justification by works; second, to show why James? developments are more fruitful starting from some of his positions in the Varieties. To carry out these aims I divide this article in four sections: first, I examine the direct influence of James on Royce?s ?The Philosophy of William James and the Philosophy of Life? in section I (After James? Death: Royce on James); then, I analyze how Royce interprets James in The Sources of Religious Insight  in section II (Beyond James: SRI and Individual?s Religious Experience); after that, I refer to some aspects of The Problem of Christianity where Jamesian issues are taken in section III (Against James: PC and the Religion of Loyalty); fourthly, I develop my hypothesis in section IV (The Battle for Justification). Finally, I present some conclusions","palabras_clave":"JOSIAH ROYCE|WILLIAM JAMES|CLASSICAL PRAGMATISM|RELIGION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53937,"idioma_id":5,"titulo":"SOMOS EL PUEBLO Y LA PATRIA. El populismo lencinista en Mendoza frente al conflicto social y la prensa: discursos, representaciones y acciones, 1917-1919","resumen":"Este artículo continúa con investigaciones en las que buscamos aportar a la reconstrucción de la historia del lencinismo en Mendoza, un fenómeno populista que tuvo amplia vigencia en la provincia durante una década larga e influyó en procesos similares en otras partes del país. Se busca, especialmente, establecer cómo evolucionó el discurso y la acción de la Unión Cívica Radical desde antes de la llegada al gobierno provincial y durante la gestión de su líder José Néstor Lencinas, en relación con los sectores subalternos de la sociedad, los conflictos sociales y la prensa.  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; El trabajo muestra el cambio que registra el discurso lencinista en la categoría pueblo, las diferentes acciones ante el conflicto social, las alianzas y los enemigos que construye. Hemos utilizado como fuentes principales la prensa de la época.","palabras_clave":"LENCINISMO|HUELGAS|PRENSA|POPULISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":53940,"idioma_id":5,"titulo":"Los frutos del viñedo deberían ser para todos. Depresión y resurrección de la vitivinicultura y aumento de la conflictividad social en Mendoza (Argentina), 1919-1920","resumen":"En este artículo se presenta la situación de la economía vitivinícola de Mendoza que, al comienzo de la Primera Guerra Mundial cayó en una profunda depresión, para reactivarse desde 1919. La gran crisis tuvo considerable impacto económico. Fue enfrentada con medidas procíclicas que incrementaron el daño. En el plano social, la gravedad del momento se hico sentir con caída de salarios y desempleo entre los sectores populares. Se creó una mal llamada Cooperativa Vitivinícola, monopolio que pasó a controlar todo el sector con la finalidad de equilibrar demanda y oferta, sin éxito.                El triunfo de la Unión Cívica Radical en enero de 1918 y la asunción del gobernador José N. Lencinas, crearon un clima político que fue favorecido por la reactivación de la economía nacional. Un discurso y una legislación obrerista crearon condiciones, junto con la intervención del Estado en la economía provincial, para que la vitivinicultura saliera de su crisis pero, simultáneamente, incentivaron la conflictividad social. Ambos fenómenos son mostrados: destrucción y regeneración de riqueza; y conflictos gremiales que involucraron, con suerte variada, al conjunto del movimiento obrero que buscaba mejores condiciones de trabajo y mejor distribución de la riqueza.          ","palabras_clave":"VITIVINICULTURA|CRISIS|REACTIVACION|HUELGAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54002,"idioma_id":5,"titulo":"Pensar la inmanencia: Gilles Deleuze y François Jullien","resumen":"El concepto de 'inmanencia' ha adquirido una creciente relevancia en el campo filosófico contemporáneo. Dos destacados pensadores franceses, Gilles Deleuze y François Jullien, le han otorgado un lugar fundamental en sus investigaciones. El propósito del presente artículo es indagar el diálogo, poco explorado aún, entre ambos autores. Nuestra hipótesis de trabajo propone los siguientes ejes de análisis: primero, comparamos los usos de la expresión 'absolutización de la inmanencia', acuñada por el sinólogo a fin de relativizar la trascendencia en el pensamiento chino y luego empleada por Deleuze en el marco de la creencia en este mundo. En segundo lugar, examinamos las críticas que Jullien le dirige a Deleuze respecto de la relación entre la filosofía y la sabiduría. Tercero, confrontamos sus perspectivas divergentes sobre la inmanencia, en tanto 'plano' (Deleuze) y como 'fondo' (Jullien). Finalmente, ponemos de manifiesto la dificultad presente en ambos autores para pensar la idea de inmanencia. ","palabras_clave":"DELEUZE|JULLIEN|INMANENCIA|SABIDURÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54004,"idioma_id":5,"titulo":"La deriva deleuziana de Roberto Esposito","resumen":"En este trabajo abordamos los conceptos de Gilles Deleuze presentes en la obra de Roberto Esposito. Tras décadas en las que el pensamiento del francés había sido poco estudiado o bien criticado por la filosofía italiana, la irrupción del paradigma biopolítico le otorgó un nuevo protagonismo a causa de su lectura de Foucault. La interpretación de Toni Negri del biopoder a través de la óptica de la sociedad de control, así como la elaboración de una biopolítica afirmativa por parte de Esposito, constituyen ejemplos de ello. Ahora bien, Esposito ha recurrido a la obra deleuziana cada vez más en sus producciones posteriores a Bíos, a propósito de temas diversos: en Terza Persona se apoya en su concepción del acontecimiento a fin de fundamentar su filosofía de lo impersonal, mientras que en Due emplea la idea de 'plano de inmanencia' para desconstruir la máquina teológico-política. Nuestro objetivo es analizar el uso espositiano de los conceptos deleuzianos en estos textos, donde vincula perspectivas del francés con problemáticas diferentes a las de los textos de origen. Esposito lleva a cabo de esta manera, antes que una lectura exegética de Deleuze, un empleo novedoso de sus nociones que le permite reformular los problemas planteados.&nbsp;&nbsp; ","palabras_clave":"VIDA|BIOPOLITICA|IMPERSONAL|INMANENCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54006,"idioma_id":5,"titulo":"Vitalismo y desubjetivación. La ética de la prudencia en Gilles Deleuze","resumen":"En el marco del debate sobre los lineamientos fundamentales de la ética de Deleuze, diversos autores (Badiou, Braidotti, Hardt, Mengue) han reparado en nociones destacadas de su obra: el deseo, el devenir, la afirmación, el nomadismo. Mi propuesta en este artículo es desarrollar una vía, poco estudiada aún, que gira alrededor de la idea de 'prudencia práctica experimental'. Según mi hipótesis, este concepto reúne perspectivas relevantes de la producción deleuzeana posterior a L´Anti-Edipe, en particular su orientación vitalista, su opción por la desubjetivación y su apelación a una dimensión ascética vinculada con el deseo como impulsor de la experimentación. ","palabras_clave":"ÉTICA|PRUDENCIA|VITALISMO|DESUBJETIVACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54088,"idioma_id":5,"titulo":"Horticultura: Una visión desde los consumidores","resumen":"El acto de consumir es parte de un ensamblaje de elementos y personas vinculados a las tramas productivas, comerciales y al mundo familiar. Si bien esta publicación se centra particularmente en dos productos hortícolas primordiales en la canasta de consumo de los argentinos (tomate y lechuga), este capítulo analizará las prácticas y las representaciones alimentarias, teniendo en cuenta al conjunto de frutas y hortalizas. La infinidad de factores que se entrelazan a la hora de decidir la compra o consumo de los productos hortícolas en general, implica abordar la temática desde un análisis interdisciplinario que incorpore aspectos económicos, sociológicos y antropológicos. El objetivo de este capítulo es avanzar en el conocimiento sobre el consumo de hortalizas en la Argentina desde una mirada más amplia, considerando al acto de consumir como un hecho social complejo. Si bien, se trata de un análisis exploratorio, se pretende ir delineando algunas ideas para pensar en políticas alimentarias que promuevan el consumo de alimentos saludables, así como permitan desarrollar estrategias para reconstruir la significancia simbólica de la alimentación y su relocalización en relación a la cultura local y territorial.","palabras_clave":"Hoticultura|Consumo alimentarios|gustos y preferencias"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54112,"idioma_id":5,"titulo":"Estudio de la contribución de nitrógeno por parte de los cultivos de cobertura al cultivo de maíz.","resumen":"La utilización de cultivos de cobertura (CC) persigue  varios objetivos como, por ejemplo, el control de  malezas (Abdin et al., 2000; Gerowitt, 2003), el  aumento de las reservas de carbono (C) de los sistemas  de producción agrícolas (Follett, 2001; Dinesh, 2004;  Ding et al., 2006), y el mejoramiento de las propiedades  físicas del suelo (Franzluebbers y Stuedemann, 2008).  En muchos casos, los CC son fertilizados para lograr  una mayor producción de biomasa y, de esta manera,  aumentar el secuestro de C (Follett, 2001) y mejorar la  cobertura del suelo. Los CC fertilizados pueden afectar  las emisiones de óxido nitroso (N2O) del suelo (Petersen  et al., 2011) pero también mejorar la disponibilidad de  nitrógeno (N) para los cultivos posteriores (Ranells y  Wagger, 1997; Mohammadi, 2010).  En ambientes semiáridos o sub-húmedos, uno de los  principales problemas para incluir los CC es su efecto  potencialmente negativo sobre la disponibilidad de  agua para los cultivos de cosecha (Restovich et al.,  2012), lo que podría reducir el rendimiento potencial.  Sin embargo, resultados preliminares indican que, en  la pampa semiárida, el impacto negativo de los CC en  el agua almacenada podría ser insignificante y no se  esperarían disminuciones significativas del rendimiento  de cultivos de cosecha (Fernández et al., 2010),  coincidiendo con experiencias en otros ambientes  semiáridos (Fengrui et al., 2000).","palabras_clave":"fertilización nitrogenada|ciclado de nutrientes|maiz"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54150,"idioma_id":5,"titulo":"Lectura y escritura mediadas por TIC. Una secuencia didáctica integradora en la formación de profesores de biología","resumen":"En este trabajo describimos una experiencia desarrollada en un Instituto Superior de Formación Docente de la Provincia de Buenos Aires, desarrollada en la asignatura Biología y Laboratorio del primer año del profesorado en Biología. Presentamos una secuencia didáctica con un enfoque sistémico y contextualizado que involucró la lectura y escritura mediadas por Tecnologías de la Información y la Comunicación (TIC) para la producción de un texto académico. Dicho texto  actuó como evaluación de la unidad cambios en la biodiversidad. Para finalizar, analizamos los logros de la secuencia propiciando una articulación entre las teorías que guiaron la planificación y lo acontecido en el aula.","palabras_clave":"Lectura|Escritura|TIC|Formación Docente"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54169,"idioma_id":5,"titulo":"Reseña a Grammático, Karin: Mujeres montoneras. Una historia de la Agrupación Evita, 1973-1974 (Buenos Aires, Ediciones Luxemburg, 2011)","resumen":"Reseña del libro de Karin Grammático Mujeres montoneras. Una historia de la Agrupación Evita, 1973-  1974, Buenos Aires, Ediciones Luxemburg, 2011, 130 págs.    Mujeres montoneras. Una historia de la Agrupación Evita de Karin Grammático es una versión de su tesis de maestría en Investigación Histórica realizada en la Universidad de San Andrés. Desde el título del libro, el lector ya puede adivinar que se encontrará con un trabajo que cruza dos campos de estudio: la historia reciente y los estudios de género. Así la investigación de Grammático hace serie con otras que, hace ya algunos años, han decidido interrogar el pasado reciente desde el lente del género, permitiendo que emerjan preguntas ligadas a la vida cotidiana de los militantes, a los roles prescriptos a hombres y mujeres, a la puesta en práctica de esos roles y a las subjetividades implicadas en los proyectos políticos de los setenta.   Desde ese campo específico de estudio, que cruza pasado reciente y género, la autora formula la tesis principal: la participación de las mujeres en la Agrupación Evita  (ya fuesen militantes montoneras o integrantes de los sectores populares) implicó una experiencia política específica que les permitió cuestionar los lugares de subordinación mantenidos tanto en sus vínculos de pareja y familiares, como en la propia organización Montoneros y en otros espacios políticos.","palabras_clave":"Montoneros|Agrupación Evita|Género|Pasado reciente"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54208,"idioma_id":5,"titulo":"Reseña del libro Yangilevich, Melina. Estado y criminalidad en la frontera sur de Buenos Aires (1850-1880)","resumen":"Añorada como un espacio de reconocimiento del avance del estado nacional en un orden social que se regía por su ausencia o discutida como un espacio de confrontación conflictiva entre las elites letradas y las voces subalternas; la ?ley? es un tema obligado en los diversos trabajos que reflexionan sobre el período post-independentista en adelante. La historia de la justicia criminal de América Latina en los últimos veinte años prueba hasta que punto la agenda historiográfica ha dejado de ser algo más que la tradicional exploración de la historia del derecho para convertirse en un espacio de convergencia e interés de los historiadores por el papel mediador de los jueces y las normas legales en las relaciones sociales, de género, de etnicidad, la sexualidad, el colonialismo, etc. en la vida cotidiana. Y consecuentemente, reconocer en los discursos sobre el desorden, los modos de vida, la rebelión de las conductas subalternas los proyectos de disciplinamiento de las elites ?incompletos o parcialmente aceptados-.","palabras_clave":"ESTADO|CRIMINALIDAD|PROVINCIA DE BUENOS AIRES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54254,"idioma_id":5,"titulo":"Del invento al medio. Del pionero al oyente","resumen":"Presentamos aquí algunas hipótesis derivadas del proyecto de investigación ?Escuchar las prácticas?, acreditado en la Universidad Nacional de Rosario.  A partir de una aproximación a los primeros años de la radiofonía en nuestro país desde la perspectiva de los oyentes, caracterizamos los espacios de producción y recepción que se fueron configurando entre las décadas del veinte y del cincuenta del siglo pasado.En principio, se hace necesaria una breve explicitación del abordaje teórico metodológico que sustenta nuestra investigación, ya que comporta una característica distintiva: reconstruimos (y de-construimos) la historia de la radio con fuentes de la historia oral, a partir de los relatos que los antiguos oyentes hacen sobre su experiencia como tales.Partiendo del análisis de esos relatos, avanzamos en la delimitación (con carácter hipotético, a esta altura del proceso investigativo) de algunas modalidades de configuración; por un lado, de la radio como medio masivo de comunicación y, por el otro,  del oyente como una construcción social inédita que dará paso a la constitución de la novel categoría de audiencia.La radio es, en su origen, un invento técnico: la radiotelegrafía sin hilos comporta la novedad de acercar voces y músicas sin cuerpo. Es un invento extraño, original, mágico, como de ensueño; pero, no obstante su carácter cuasi para-normal, prontamente accesible. A poco tiempo de empezar a sonar, la radiodifusión se convirtió en la radio, ese artefacto cultural de presencia cotidiana en el ámbito familiar y que en pocos años sería protagonista del pasaje de una cultura basada en lo cercano, a una cultura formateada por la mediatización. En el campo de la recepción, paralelamente, el pasaje se produjo desde aquellos pioneros iniciados en el manejo de una técnica ?moderna?, a un saber hacer que se fue insertando en el espacio doméstico de las prácticas. Las nuevas configuraciones simbólicas que produjo la consolidación del medio, anclado ya en las culturas populares, fueron delineando el perfil de una ?especie? nueva: el oyente de radio, como miembro de una audiencia.","palabras_clave":"Radio|Historias|Relatos|Audiencia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54326,"idioma_id":5,"titulo":"Sociedad sin clases e ideologías del desarrollo. Aportes de Franz Hinkelammert al pensamiento crítico latinoamericano","resumen":"El presente texto pretende rescatar los aportes de Franz Hinkelammert, cientista social alemán radicado en América Latina desde la década del sesenta. Nos centraremos en la producción teórica llevada a cabo en su etapa chilena (1963-1973) señalando: a) su crítica a los conceptos implícitos de desarrollo y subdesarrollo presentes en las ideologías liberal-capitalista y socialista y b) el análisis de las posibilidades reales de un socialismo latinoamericano más allá de los errores y olvidos de las experiencias socialistas anteriores. Recuperar estos aportes permitiría indagar su relevancia para el actual proceso histórico latinoamericano.","palabras_clave":"DESARROLLO|SUBDESARROLLO|SOCIALISMO|HINKELAMMERT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54528,"idioma_id":5,"titulo":"Agroindustrias familiares en Misiones. Fábricas de ladrillo y almidón de mandioca de alemanes-brasileños (1919-2009)","resumen":"Este trabajo tiene como objetivo estudiar las agroindustrias familiares puestas en marcha por los migrantes alemanes-brasileños que se establecieron principalmente en la colonia Puerto Rico, Departamento Libertador General San Martín, Provincia de Misiones (1919-2009). Si bien su estrategia básica fue la autosufi ciencia familiar a través de pequeñas explotaciones agrícolas, desde principios del siglo XX implementaron la industrialización de la materia prima o de productos primarios que tenían disponibles en su propia chacra. En esta investigación se presentan estudios de caso que analizan el carácter diversificado de las estrategias de estos colonos y se describe el proceso de elaboración de las fábricas de ladrillo y almidón de mandioca.","palabras_clave":"alemanes-brasileños|agroindustrias|fábricas de ladrillo|fábricas de almidón de mandioca"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54584,"idioma_id":5,"titulo":"Niñez, migración y derechos: aportes para un abordaje antropológico.","resumen":"Este ensayo analiza contextos y presenta conceptualizaciones en torno a los discursos de derechos de niñez migrante en el ámbito argentino. Los primeros apartados del trabajo ofrecen una sistematización de los contextos regionales en los que se institucionalizan estos discursos, incluyendo los problemas y demandas que se construyen desde los enfoques de derechos. En los tres apartados finales, se propone considerar otras dimensiones de análisis, orientadas a conceptualizar a la niñez migrante en relación con las políticas hacia las familias, las infancias diversas, las tensiones implícitas en las dinámicas de particularización de sujetos de derechos. El objetivo que atraviesa la propuesta es introducir problematizaciones y niveles de análisis desde la antropología, respecto de una temática atravesada por el enfoque normativo.","palabras_clave":"Niñez|Migración|Derechos|Antropológico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54588,"idioma_id":5,"titulo":"Antropología y teatro.Dramatizaciones en una etnografía con niños y niñas migrantes","resumen":"Este artículo avanza en la precisión teórica y metodológica de una estrategia de dramatización que hemos desarrollado en el marco de nuestra investigación doctoral. Nos dedicaremos a reconstruir algunos antecedentes  de cruces entre antropología y teatro, pero también de las perspectivas más amplias sobre la dimensión del cuerpo con las que aquellos antecedentes dialogan. Dentro de este marco nos preguntaremos especialmente por el potencial de los abordajes históricos de la educación en sensibilidades de los dispositivos escolares modernos.","palabras_clave":"Antropología|Teatro|Niños|Migrantes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54611,"idioma_id":4,"titulo":"Carbohydrate reserve status of Malbec grapevines after several","resumen":"Background and Aims: Regulated deficit irrigation (RDI) and bunch thinning are two viticultural practices applied worldwide. There is limited knowledge, however, about their combined effects on carbohydrate dynamics and accumulation.We evaluated during year 3 and 4 of the experiment the effect of 4 consecutive years of RDI and bunch thinning on carbohydrate status, vegetative and reproductive variables. Methods and Results: From 2006\/07 to 2009\/10, we imposed&nbsp;four levels of water supply [100, 60, 38 and 25% of reference evapotranspiration (ETo)] and two crop loads (100 and 50% of the bunches).We compared shoot length, flowers per inflorescence and yield in 2009\/10. We also measured pruning mass and the concentration of nonstructural carbohydrates in dormant wood in the winters of 2009 and 2010. Starch concentration in the trunk was reduced by severe water deficit (25 and 38% ETo) and improved by bunch thinning. Pruning mass, shoot length, flowers per inflorescence and yield were affected in vines with water applied at 25 and 38% of ETo. Conclusions: Severe water stress and high crop load reduced trunk starch concentration with no interaction between both factors, whereas the concentration of total non-structural carbohydrate was not affected. Vegetative growth and yield were reduced after 4 years of severe water stress. Significance of the Study: We provide evidence that starch concentration and carbon partitioning can be manipulated through common viticultural practices, such as irrigation and crop load. Regulated deficit irrigation (RDI) and bunch thinning are two viticultural practices applied worldwide. There is limited knowledge, however, about their combined effects on carbohydrate dynamics and accumulation.We evaluated during year 3 and 4 of the experiment the effect of 4 consecutive years of RDI and bunch thinning on carbohydrate status, vegetative and reproductive variables. Methods and Results: From 2006\/07 to 2009\/10, we imposed&nbsp;four levels of water supply [100, 60, 38 and 25% of reference evapotranspiration (ETo)] and two crop loads (100 and 50% of the bunches).We compared shoot length, flowers per inflorescence and yield in 2009\/10. We also measured pruning mass and the concentration of nonstructural carbohydrates in dormant wood in the winters of 2009 and 2010. Starch concentration in the trunk was reduced by severe water deficit (25 and 38% ETo) and improved by bunch thinning. Pruning mass, shoot length, flowers per inflorescence and yield were affected in vines with water applied at 25 and 38% of ETo. Conclusions: Severe water stress and high crop load reduced trunk starch concentration with no interaction between both factors, whereas the concentration of total non-structural carbohydrate was not affected. Vegetative growth and yield were reduced after 4 years of severe water stress. Significance of the Study: We provide evidence that starch concentration and carbon partitioning can be manipulated through common viticultural practices, such as irrigation and crop load. ","palabras_clave":"carbohydrate reserves|crop load|deficit irrigation|malbec"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54706,"idioma_id":5,"titulo":"Setenta y 4  o el lugar de la poesía","resumen":"Reseña crítica del libro Setenta y 4 (2011) de José María Pallaoro. Se analizan las lecturas políticas del libro, específicamente la comparación entre el peronismo clásico y el gobierno kirchnerista.","palabras_clave":"LITERATURA ARGENTINA|PERONISMO|POESÍA PLATENSE|POLÍTICA NACIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54793,"idioma_id":5,"titulo":"?Una mirada sobre lo popular. Reflexiones sobre el etnocentrismo en el nuevo cine argentino?","resumen":"La emergencia del nuevo cine argentino desata una serie de debates teóricos. Probablemente   una de las caracterizaciones más convincentes es la que lo define como surgido de una misma   crisis, no sólo en el campo cinematográfico, sino también de una misma crisis social. En el   marco de un análisis sociológico de este fenómeno cultural, el presente trabajo planteará una   hipótesis de lectura para algunas de las producciones audiovisuales del denominado nuevo cine   argentino, en pos de establecer conceptualmente la estructuración desde el polo productivo de la   elaboración imaginaria de esta crisis social, concretamente, de la emergencia de las clases   populares en sus filmes. Para finalizar, se evaluará la productividad de la hipótesis de lectura   basada en una definición teórica de lo popular, aplicándola a un cuerpo de filmes del realizador   argentino Adrián Caetano, para concluir con reflexiones sobre la importancia del cruce entre la   estructuración textual y la estructuración en el momento del consumo.","palabras_clave":"sectores populares|cine|postconvertibilidad argentina|clases sociales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54797,"idioma_id":5,"titulo":"Cultura «para» el trabajo. El capital cultural y su lógica en las estrategias laborales de jóvenes de un barrio popular de Córdoba Capital","resumen":"El diagnóstico de la problemática laboral para jóvenes está generalmente asociada a dos dimensiones: la primera, referida a las cuestiones vinculadas a la ?cultura del trabajo? y su desestructuración tras años de desempleo masivo; y la segunda, referida a los problemas de calificación.  Sin embargo, limitados por enfoques ?legitimistas?, muchos de estos diagnósticos desconocen la existencia de lógicas específicamente populares de funcionamiento del capital cultural, de espacios diferenciales y de apuestas vinculadas a disposiciones singulares. El presente texto pretende discutir, desde la perspectiva de la cultura popular, la categoría de «capital cultural» aplicada a la comprensión de las estrategias laborales de jóvenes de un barrio popular de la ciudad de Córdoba.","palabras_clave":"estrategias laborales|capital cultural|cultura laboral"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54811,"idioma_id":5,"titulo":"El problema de las clases naturales en química: consideraciones acerca del esencialismo kripkeano desde la filosofía de la química","resumen":"El objetivo del presente trabajo consiste en analizar y cuestionar algunas tesis esencialistas respecto del problema de las clases naturales, en particular las tesis semánticas y metafísicas de Saul Kripke, las cuales afirman que existen clases naturales que poseen propiedades esenciales científicamente descubiertas. Los argumentos que el trabajo expone parten de considerar la práctica científica química y los desarrollos en filosofía de la química. Como aporte positivo al problema de las clases naturales en química, el trabajo persigue defender la viabilidad de una posición alternativa al esencialismo sin caer en el convencionalismo: un pluralismo ontológico con raíces kantianas.","palabras_clave":"CLASES NATURALES|FILOSOFÍA DE LA QUÍMICA|ESENCIALISMO|PLURALISMO ONTOLÓGICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54901,"idioma_id":5,"titulo":"Violaciones a los derechos humanos en el conflicto armado: Corte IDH, ?Caso Masacre de Santo Domingo vs. Colombia?","resumen":"La Corte Interamericana de Derechos Humanos dictó en 2012 una nuevasentencia contra el Estado colombiano en el caso masacre de Santo Domingo,que se suma a las reiteradas decisiones de este alto tribunal en casosatinentes a masacres en las que han participado miembros de la fuerzapública colombiana contra la población civil. Esta sentencia se dictó después14 años de ocurridos los hechos. El Estado colombiano, en este caso,violó los derechos humanos de una población rural bombardeando sus casasy sometiéndolos a situaciones de violencia y desplazamiento forzado. En este escrito se analiza la sentencia de la Corte.","palabras_clave":"Conflicto armado|Derechos Humanos|Corte Interamericana|Masacre"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54995,"idioma_id":5,"titulo":"Los hongos en el laboratorio: de la naturaleza al cultivo axénico.","resumen":"las investigaciones pueden ser encaradas desde la fisiología, taxonomía, biogeografía, etc. Para llevar adelante cualquiera de estos estudios los micólogos necesitan trabajar con cultivos puros de hongos y además con materiales de herbario. Por lo tanto el primer paso es la recolección de ejemplares a partir de los cuales se intentará lograr cultivos axénicos (libres de contaminación) y luego estos ejemplares serán secados convenientemente para depositarlos en herbarios. Las investigaciones en fisiología y biotecnología, como por ejemplo el estudio de la regulación de la producción de enzimas o la optimización de la producción de antibióticos, necesitan de cultivos puros; mientras que aquellos estudios donde se explore la biología o la ecología de algún grupo de hongos, necesitan además de los herbarios. En esta nota se describe someramente el trabajo de los micólogos en obtener cultivos puros y especímenes de herbario","palabras_clave":"HONGOS|CULTIVO|AISLAMIENTO|AXÉNICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":54998,"idioma_id":5,"titulo":"Degradación de colorantes trifenilmetánicos por aislamientos fúngicos del bosque andino patagónico en viruta de pino.","resumen":"Se evaluó la capacidad de degradación de los colorantes verde de malaquita y violeta de genciana por parte de sistemas consistentes en viruta de pino de producción local como medio de cultivo, y aislamientos de hongos de los bosques andinopatagónicos. Se determinó que cepas de Agaricus bitorquis, Gymnopilus spectabilis y una bolbitiacea no identificada decoloraron eficientemente al menos uno de los colorantes en medio agarizado. Se obtuvo un alto porcentaje de degradación (a) y detoxificación (b) de verde de malaquita 50 &#956;M por G. spectabilis (a 87%;b 86%), por la bolbitiacea (a 95%;b 93%) y una cepa de Ganoderma applanatum (a 81% ;b 59%) del cepario de CIEFAP utilizando el medio viruta luego de 30 días de cultivo.","palabras_clave":"Trifenilmetanos|Agaricus|Gymnopilus|Ganoderma"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55014,"idioma_id":5,"titulo":"'Las patas en la fuente y la comunidad organizada: dislocación comunitaria e identidad en los orígenes del peronismo'","resumen":"El presente trabajo se concentra en el proceso de constitución identitaria del peronismo, retomando el lugar que ocupa la identidad en determinados estudios canónicos sobre los orígenes del peronismo y en clave de respuesta a los límites de las explicaciones basadas en el racionalismo instrumental. A partir de allí, se indaga en la conformación de un nuevo sujeto popular, articulado a partir de la interpelación peronista e identificado en la representación con Perón, cuya emergencia agrietó decisivamente el orden tradicional. Dicho proceso se plasmó en la llamada ?crisis de la deferencia?, entendida como el fin de la aceptación de un lugar establecido en un orden hegemónico, expresando así un quiebre de los lugares asignados previamente en la comunidad política. Esta dislocación comunitaria de origen marcó constitutivamente al régimen peronista ?donde convivieron tensamente tendencias orientadas al orden y a la ruptura? atentando contra los intentos de sutura estatal de aquella ruptura inicial. Esta última reflexión es guiada por el aporte de una serie de teorizaciones sobre el populismo como matriz específica en el marco de las identidades políticas.","palabras_clave":"PERONISMO|IDENTIDADES POLITICAS|DEFERENCIA|POPULISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55141,"idioma_id":4,"titulo":"Photochemical Reaction Engineering","resumen":"A brief presentation of photoreactor engineering theory is rigorously derived from chemical reaction engineering principles and radiation energy transport fundamentals. The analysis is illustrated by presenting an example of the nowadays most significant contribution of this field aimed at environmental remediation. Accordingly, the modeling of an annular reactor inside a recycle for a typical photocatalytic decomposition reaction is succinctly described. In order to formulate the photochemical reaction rate properly, the radiative transfer equation applied to participating and reactive, homogeneous and heterogeneous media is presented. The described approach provides the most important tools for mathematical modeling of photoreactions and photoreactors that permits a rigorous and practical utilization of this methodology. Thus, if and only if, well designed laboratory experimental data are obtained, an a priori scale-up procedure can be performed resorting to these concepts with no limitations in the size or shape of virtually any form of commercial applications.","palabras_clave":"Photocatalysis|Reactor Design|Mathematical Modeling|Photochemical Degradation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55175,"idioma_id":5,"titulo":"Perspectivas para el análisis de la acción colectiva: algunas reflexiones críticas y posibles aportes desde la teoría de la hegemonía","resumen":"En este artículo se intenta reflexionar sobre algunas perspectivas empleadas para el análisis de la acción colectiva. La reconstrucción del estado de la cuestión permitió, por un lado, identificar elementos que resultarían pertinentes para el abordaje de diversas experiencias de acción colectiva en América Latina de fines del siglo XX y comienzos del XXI. Por otro lado, dicha sistematización puso en evidencia las limitaciones de las miradas predominantes para abordar el carácter no homogéneo de las identidades y su dinamismo constante a partir de prácticas articulatorias.En esa línea se retoman algunas categorías de la teoría de la hegemonía, desde Antonio Gramsci hasta Ernesto Laclau y Chantal Mouffe, que resultan de suma utilidad para clarificar el sentido de la movilización social contemporánea.","palabras_clave":"ACCION COLECTIVA|AMERICA LATINA|TEORIA DE LA HEGEMONIA|IDENTIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55211,"idioma_id":4,"titulo":"Ionically conducting ceramics as active catalyst supports","resumen":"Electrochemistry and heterogeneous catalysis on metals are usually treated as different branches of physical chemistry, while similar concepts are developed to model their kinetics and similar surface science techniques are used to investigate their fundamental aspects at the atomic level. There is thus increasing evidence that they are much more closely related than originally thought. The situation is to some extent analogous to that existing between electrochemistry and corrosion science many decades ago. The urgent need for developing economically viable fuel cells has brought the electrochemical and catalytic communities closer than ever before. Ionically conducting ceramics have played and will play a significant role in this direction.","palabras_clave":"IONICALLY CONDUCTING CERAMICS|CATALYSIS SUPPORTS|ELECTROCHEMICAL PROMOTION OF CATALYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55221,"idioma_id":4,"titulo":"Ketamine prevents seizures and reverses changes in muscarinic receptor induced by bicuculline in rats.","resumen":"The cholinergic system has been implicated in several experimental epilepsy models. In a previous study bicuculline (BIC), known to antagonize GABA-A postsynaptic receptor subtype, was administered to rats at subconvulsant (1 mg\/kg) and convulsant (7.5 mg\/kg) doses and quinuclidinyl benzilate ([3H]-QNB) binding to CNS membranes was determined. It was observed that ligand binding to cerebellum increases while it decreases in the case of hippocampus. Saturation binding curves showed that changes were due to the modification of receptor affinity for the ligand without alteration of binding site number. The purpose of this study was to assay muscarinic receptors employing other BIC dose (5 mg\/kg), which induces seizures and allows the analysis of a postseizure stage as well. To study further muscarinic receptor involvement in BIC induced seizures, KET was also employed since it is a well known anticonvulsant in some experimental models. The administration of BIC at 5 mg\/kg to rats produced a similar pattern of changes in [3H]-QNB binding to those recorded with 1.0 and 7.5 mg\/kg doses. Here again, changes were observed in receptor binding affinity without alteration in binding site number for cerebellum or hippocampus membranes. Pretreatment with KET (40 mg\/kg) prevented BIC seizures and reverted [3H]-QNB binding changes induced by BIC administration. The single administration of KET invariably resulted in [3H]-QNB binding decrease to either cerebellar or hippocampal membranes. KET added in vitro decreased ligand binding likewise. Results of combined treatment with KET plus BIC are hardly attributable to the single reversion of BIC effect since KET alone invariably decreased ligand binding. It is suggested that besides alteration of cholinergic muscarinic receptor other(s) neurotransmitter system(s) may well also be involved.","palabras_clave":"KETAMINE|BICUCULLINE|EXPERIMENTAL EPILEPSY|MUSCARINIC RECEPTORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55228,"idioma_id":4,"titulo":"The influence of different contraceptive methods on vaginal microbiota: Clinical study","resumen":"Objective: The aim of this work was to evaluate the vaginal content of women attending family planning controls and its relationship with the contraceptive practice used (oral contraceptive pills, intrauterine device, condoms, or rhythm method) as well as its relationship with the lack of regular contraception. Design: observational, descriptive study. Setting: Servicio de Atención Médica - Ministerio de Salud - Sa Pereira - Santa Fe. Argentina.Population: A total of 250 women were studied following the BAVACO (balance of the vaginal content).Methods: Standard method, which included the wet mount test, and Giemsa and Gram stain under the Nugent score.Results: Obtained from the vaginal microbiota, the vaginal inflammatory response, presence of yeast and Trichomonas allowed defining five basic vaginal microbial states which were statistically related to contraceptive methods.Main Outcome Measures: The association of each of the five vaginal states with the different contraceptive 2 practices was determined by X test, considering p Conclusions: a) the positive association of combined oral contraceptives with a normal microbiota indicates a positive trend of protection, b) intrauterine devices increase whereas oral contraceptives decrease the risk for bacterial vaginosis, while oral contraceptives reduce the frequency.","palabras_clave":"Vaginal Dysfunction|Vaginal Content|Vaginal States|Contraception"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55420,"idioma_id":4,"titulo":"Applying MDE tools to defining domain specific languages for model management","resumen":"In the model driven engineering (MDE), modeling languages play a central  role. They range from the most generic languages such as UML, to more individual ones, called domain-specific modeling languages (DSML). These languages are used to create and manage models and must accompany them throughout their life cycle and evolution.  In this paper we propose a domain-specific language for model management, to facilitate the user´s task, developed with techniques and tools used in the MDE paradigm.","palabras_clave":"modeling language|metamodels|transformation language"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55482,"idioma_id":5,"titulo":"Desbalance entre el sistema pro y antioxidante en pulmon de animales castrados y acción de hsp27 y hsp70.","resumen":"Introducción: El estrés oxidativo tiene un rol importante en númerosas patologías pulmonares. Además, hay numerosas evidencias que revelan la acción de las proteínas de golpe de calor (HSP),  principalmente en cuadros inflamatorios.  Sumado a ello la presencia de receptores de andrógenos (RA) en pulmón implicaría la acción de hormonas sexuales, que jugarían un rol fisiológico en el órgano.Objetivo: Nuestro objetivo fue investigar el rol de las HSP 27 y 70i en pulmón de animales castrados asociado al comportamiento de los componentes del sistema de defensa antioxidante. Objetivo secundario, determinar si la situación puede ser revertida con administración testosterona.Materiales y Métodos: Se usaron ratas Wistar (200 ± 20g) las cuales fueron separadas en 3 lotes: controles (Co), castradas (Ca) y castradas con Testosterona (Ca+T) que se sacrificaron a los 30 días. Se determinó el grado de lipoperoxidación cuantificando (TBAR´S). Se extrajo RNA total, se obtuvo ADNc  y se realizaron PCR, usando como control interno S28. Se investigó la expresión de Hsp 27 y 70i por inmunohistoquímica (IHQ). Asimismo se determino la actividad de CAT y Gpx en homogenatos de pulmón.Resultados: En el grupo Ca, aumentaron los niveles de RA (p<0,01), TBARS (p<0,01) al igual que las actividades de las enzimas CAT (p<0.01) y GPx (p<0,05). También aumentó la expresión de GPx (p<0,05), Nox (p<0.01) y NRF-2 (p<0,05). TNF-alfa e IFN-gamma disminuyeron su expresión (p<0,05). Se incrementó la inmunomarcación positiva para Hsp 70 en Ca (p<0,01), mientras que Hsp 27 disminuyó (p<0,001). Ca + T revertiría el cuadro en algunos parámetros.Conclusión: Nuestro modelo experimental estaría demostrando un desbalance entre el sistema pro y antioxidante, con un cuadro inflamatorio al que el organismo trata de resolver. Sumado a ello, las HSP estarían asociadas a una respuesta de protección ante la injuria producida.","palabras_clave":"ESTRES OXIDATIVO|PULMON|CASTRACION|RATAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55487,"idioma_id":4,"titulo":"Is the time an important variable in the effect of the androgen deprivation in the lung","resumen":"Lungs are susceptible to injury due to free radicals. Also, androgensplay a physiological role in pulmonary function. We tested theeffect of castration on the prooxidant-antioxidant balance at twodifferent times: 30 and 60 days after castration.Wistar male rats (200±20g) were separated in three groups: controls(Co), castrated (Ca), and castrated supplemented with testosterone(Ca+T) for five days. After 30 (30d) or 60 days (60d) rats werekilled. TBAR?S and CAT activity were determined. Total RNAwas isolated and Androgen Receptor (AR), Superoxide Dismutase(SOD), Nuclear factor-erythroid 2 related factor 2 (Nrf-2),Glutathion peroxidase (GPx) and NADPH Oxidase (NOX-2) weredetermined by PCR. S28 was the control.The expression of AR showed an increase in Ca and a significantdecrease in Ca+T in both time (p<0.05). TBAR?S and CAT activityincreased in Ca in both groups (p<0.05). GPx activity increased at30d (p<0.001), did not change at 60d as well at its expression.Nrf-2 expression increased in Ca at 30d but only increased in Ca+Tat 60d (p<0.05). SOD did not change at 30d but increased in Ca at60d (p<0.05). NOX increased at 30d but decreased in Ca (p<0.05)at 60d. CT expression did not change. We conclude that androgendeprivation alters the prooxidant-antioxidant balance in lung in atime-dependent manner.","palabras_clave":"LUNG|FREE RADICAL|ANDROGENS|CASTRATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55499,"idioma_id":5,"titulo":"Aportes de Arturo Roig para una teoría crítica del humanismo","resumen":"El artículo dirige su atención a un texto de Arturo Roig que ha sido considerado como una de las obras más relevantes de nuestro pensamiento, Toría y crítica del pensamiento latinoamericano (1981). Orientamos nuestra indagación hacia los ejes que muestran los rasgos humanísticos de su pensamiento: la relación entre teoría y crítica, la categoría de a priori antropológico, categoría desde la cual considera la cuestión del sujeto, y la noción de reconocimiento en su propuesta filosófica.&nbsp;","palabras_clave":"TEORÍA CRÍTICA|A PRIORI ANTROPOLÓGICO|ARTURO ROIG|RECONOCIMIENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55521,"idioma_id":4,"titulo":"Phylogenetic analysis of the genus Modicella reveals an independent evolutionary origin of sporocarp forming fungi in the Mortierellales","resumen":"Most studies of tissue differentiation and development have focused on animals and plants but many  fungi form multi-cellular aggregations of spore-bearing tissue known as fruiting bodies or sporocarps.  The ability to form sporocarps has arisen independently in several different evolutionary lineages of  fungi. Evolutionary relationships of most sporocarp-forming fungi are well known, but the enigmatic zygomycete  genus Modicella contains two species of sporocarp-forming fungi for which the phylogenetic  affinities have not been explored based on molecular data. Species of Modicella have an uncertain trophic  mode and have alternatively been considered members of the order Endogonales (which contains documented  species of sporocarp-forming fungi) or the order Mortierellales (which contains no previously  documented species of sporocarp-forming fungi). In this study we perform phylogenetic analyses based  on ribosomal DNA of Modicella malleola from the Northern Hemisphere and Modicella reniformis from the  Southern Hemisphere to determine the evolutionary affinities of the genus Modicella. Our analyses indicate  that Modicella is a monophyletic genus of sporocarp-forming fungi nested within the Mortierellales,  a group of microfungi with no previously documented sporocarp-forming species. Because Modicella is  distantly related to all other known sporocarp-forming fungi, we infer that this lineage has independently  evolved the ability form sporocarps. We conclude that the genus Modicella should be a high priority for  comparative genomics studies to further elucidate the process of sporocarp formation in fungi.","palabras_clave":"ENDOGONALES|ENDOGONE|FRUITING BODY|PHYLOGENY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55567,"idioma_id":5,"titulo":"?Contribuiciones antropologicas para las intervenciones de desarollo rural em Argentina?,","resumen":"Reflexionamos sobre el reconocimiento por parte del Estado argentino de la diversidad multicultural de los productores agropecuarios, que conlleva nuevas formas de políticas de desarrollo rural y el desafío de incorporar nuevos saberes a los proyectos de intervención, considerando el multiculturalismo como matriz de estas políticas y la necesidad de empoderar a los beneficiarios, a través de la utilización de la planificación participativa en el diseño, gestión, implementación y evaluación de estas estrategias de intervención.  La Antropología cuestiona los prejuicios de los extensionistas respecto de los saberes locales de los beneficiarios, en tanto permite dialogar, aprender y construir conocimientos conjuntamente. En proyectos de intervención rural, a través del uso de herramientas etnográficas, el facilitador puede recorrer un camino entre un marco conceptual metodológico cuantitativo hacia uno cualitativo, rescatando su propia participación en la vida cotidiana de la población, la interacción y la práctica reflexiva, como medios para visualizar la realidad ?desde adentro?. Los descubrimientos etnográficos en dominios específicos, deben ser aplicables a las políticas de desarrollo","palabras_clave":"DESARROLLO|POLITICAS PUBLICAS|ENFOQUE ANTROPOLOGICO|ETNOGRAFIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55626,"idioma_id":5,"titulo":"EVALUATION OF WATER USE EFFICIENCY AND PRODUCTIVITY IN WINTER CROPS OF THE SEMIARID PAMPA","resumen":"El incremento de la eficiencia de uso de agua (EUA) en agricultura se ha convertido enun tema primordial. Asimismo, al comenzar a considerarse el va lor económico del producto,s e des arrolló el concept o de productivida d econ ómica del a gua (PEA), definidocomo el valor recibido por unidad de agua usada (U$s\/mm). Dicho objetivo puede alcanzarseya sea por un incremento en la EUA como por la producción de cultivos de más elevado va lor. En la EEA INTA Anguil, La P ampa, se están lleva ndo a cabo ensayos condiversos cultivos invernales (trigo s grupo de calidad industrial 1 y 3, cebada, cártamo ycolza) con el objetivo de identificar aquellos que brindan la mejor EUA y PEA. Los resultadosde la campaña 2011 demostraron que la cebada tuvo la más alta EUA (14,4 kg\/mm)y PEA (2,6 U$s\/mm ). Los trigos presentaron EUA variables entre 10,9-13,6 kg\/mm, correspondiendolos valores más altos al grupo 3, en cuanto a PEA no hubo diferencias significativasentre calidades (1,5 a 1,8 U$s\/mm). Las oleaginosas demostraron muy buencomportamiento, con EUA de 4,8 kg\/mm en colza y 5,6 kg\/mm en el caso del cártamo, yla PEA de ambas oleaginosas fue de alrededor de 2 U$s\/mm. Si bien los resultados sonpreliminares, las oleaginosas inverno-primaverales parecen alternativas interesantes paraincorporar en las rotaciones.","palabras_clave":"TRIGO|CEBADA|COLZA|CARTAMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55663,"idioma_id":5,"titulo":"Dilemas éticos y modelos deontológicos para el periodista usuario de medios sociales","resumen":"El artículo se propone identificar los principales dilemas éticos emergentes en el uso de medios sociales por parte de los periodistas. Para ello, recorre experiencias recientes que dieron lugar a reflexiones sobre la necesidad de establecer pautas específicas para la publicación de contenidos en estos medios, y analiza las características y la pertinencia de las respuestas deontológicas a esos dilemas, ensayadas en los últimos dos años por distintas organizaciones periodísticas de proyección internacional. Finalmente, se clasifican y describen los modelos deontológicos de acuerdo con el grado de autonomía que las organizaciones conceden al periodista para que se exprese libremente en los medios sociales, y se plantea la necesidad de la autorregulación individual y de fortalecer el compromiso del periodista con la excelencia profesional.","palabras_clave":"PERIODISMO|MEDIOS SOCIALES|ETICA|AUTORREGULACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55694,"idioma_id":4,"titulo":"Efficient expression systems for cysteine proteases of malaria parasites: too good to be true?","resumen":"Papain-like cysteine proteases of malaria parasites are considered important chemotherapeutic targets or valuable models for the evaluation of drug candidates. Consequently, many of these enzymes have been cloned and expressed in Escherichia coli for their biochemical characterization. However, their expression has been problematic, showing low yield and leading to the formation of insoluble aggregates. Given that highly-productive expression systems are required for the high-throughput evaluation of inhibitors, we analyzed the existing expression systems to identify the causes of such apparent issues. We found that significant divergences in codon and nucleotide composition from host genes are the most probable cause of expression failure, and propose several strategies to overcome these limitations. Finally we predict that yeast hosts Saccharomyces cerevisiae and Pichia pastoris may be better suited than E. coli for the efficient expression of plasmodial genes, presumably leading to soluble and active products reproducing structural and functional characteristics of the natural enzymes.","palabras_clave":"CYSTEINE PROTEASES|MALARIA PARASITES|EFFICIENT EXPRESSION SYSTEMS|CODON OPTIMIZATION|AUTO-INDUCTION|ESCHERICHIA COLI  |PICHIA PASTORIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55725,"idioma_id":5,"titulo":"¿Cómo se toman decisiones en el FMI? La incidencia de los países centrales en el otorgamiento del 'blindaje' a la Argentina en 2001","resumen":"Si bien existe amplia evidencia de la incidencia de los gobiernos de los países centrales sobre el comportamiento del FMI, surgen interrogantes respecto de cómo se resuelven los conflictos entre ellos, y cuál es el alcance que poseen sobre la acción del Fondo. En el presente artículo se proponen dos objetivos. Primero, se examina la pertinencia y relevancia de las Minutas de la reunión del Directorio Ejecutivo del FMI como fuente primaria para el análisis de la incidencia de los intereses de los países centrales sobre el organismo. Segundo, a partir de los testimonios contenidos en la Minuta de la reunión del 12 de enero de 2001 en la cual se decidió la aprobación del 'blindaje', se analiza la posición de los países del G7 en relación a la continuidad de la convertibilidad. Se identifican cuatro posiciones que reflejan diferencias al interior de ese bloque, destacándose las posiciones críticas.","palabras_clave":"Directorio Ejecutivo del FMI|Argentina|Blindaje|G7"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55732,"idioma_id":4,"titulo":"Screening of vip genes from a Spanish Bacillus thuringiensis collection and characterization of two Vip3 proteins highly toxic to five lepidopteran crop pests","resumen":"A Spanish Bacillus thuringiensis strain collection was screened for the presence of vip genes. One hundred strains from a Canary Island collection were screened for vip1 and vip2 genes and 7% contained poten- tially novel vip1 and vip2-like genes, as indicated by the low degree of similarity with previously known vip1 and vip2 genes. Four hundred strains from a collection originating from the Spanish mainland were screened for vip3 genes and 14.5% of them contained potentially novel vip3-like genes. Reconstruction of the full-length vip sequences could only be achieved for two vip3 gene variants encoding 789 and 787 amino acid proteins that were designated as Vip3Aa45 and Vip3Ag4, respectively. These proteins showed 82% pairwise identity between them and differed from Vip3Aa1 in the putative signal peptide, two specific proteolytic processing sites and the 66-kDa insecticidal fragment. The purified proteins were tested against nine lepidopteran pest species and displayed toxicity, expressed as mean lethal concentration, for five of them. The two toxins were highly toxic for Lobesia botrana (&#1113093;1?2 lg\/ml) and Spodoptera littoralis (&#1113093;20 ng\/cm2), moderately toxic for Spodoptera exigua (&#1113093;100?300 ng\/cm2), and varied greatly in their toxicity for Mamestra brassicae or Chrysodeixis chalcites, with high toxicity for Vip3Aa45 in M. brassicae (&#1113093;40 ng\/cm2) and for Vip3Ag4 in C. chalcites (&#1113093;45 ng\/cm2).","palabras_clave":"BACILLUS THRINGIENSIS|VEGETATIVE INSECTICIDAL PROTEINS|LEPIDOPTERAN PESTS|BIOLOGICAL CONTROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":55809,"idioma_id":5,"titulo":"Paisaje ritual y marcadores astronómicos en el sitio Uña Tambo, Nevados de Cachi, Salta, Argentina","resumen":"En este trabajo daremos cuenta de las nuevas investigaciones realizadas en los Nevados de Cachi, Salta,Argentina focalizándonos en el sitio que hemos denominado Uña Tambo, y cuál fue su integración dentro de la red de sitios inkaicos de la región. En esta ocasión haremos hincapié en algunos aspectos arqueoastronómicos del asentamiento, sin dejar de lado la importancia visual y simbólica que tuvo la ubicación de esta clase de sitios así como también la relevancia de los mismos dentro de los rituales inkaicos relacionados con el agua, la agricultura y con el culto a los Apus.","palabras_clave":"INKA|APUS|PAISAJE|ARQUEOASTRONOMIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56023,"idioma_id":4,"titulo":"WITHDRAWAL TIME OF FOSFOMYCIN IN PIGS AND BROILER CHICKENS AFTER ORAL AND INTRAMUSCULAR ADMINISTRATION","resumen":"Fosfomycin (FOS) is a broad-spectrum bactericidal antibiotic, used for the treatment and prevention of pulmonary and enteric infections in poultry and intensive pig farming. FOS pharmacokinetics has been extensively studied in humans and animals; although its withdrawal time (WDT) has not been determined yet. This study was aimed to determine WDTs of calcium and disodium FOS in pigs and broiler chickens. For residue analysis and WDT calculation, 48 healthy, 145-150 day-old pigs and 48 healthy, 14 day-old, broiler chickens, were used. In both species, animals were divided in two groups (A and B). Groups A - Oral Assays: calcium FOS, 5 consecutive days; 30 mg\/kg pv, (pigs) and 40 mg\/kg pv (broiler chickens). On the fifth day of trial, FOS dosage was discontinued. Groups B - Intramuscular Assays: disodium FOS; 15 mg\/kg pv (pigs) and 10 mg\/kg pv (broiler chickens). In all assays, 24, 48, 72 and 96 h after dosage discontinuation, groups of 6 animals were euthanized. Samples of muscle, liver, kidney and skin-fat were obtained and processed for the determination of residual concentrations of FOS by HPLC MS-MS. WDTs were calculated by the statistical program WTM 1.4. The longer WDT were 2.95 and 2.55 days (kidney) for the oral administrations and 1.73 (liver) and 1.72 days (muscle), for the intramuscular administrations, in pigs and broiler chickens, respectively. Therefore, WDTs of 3 days (after oral administrations) and 2 days (after intramuscular administrations), could be assigned as a precautionary principle for public health, without a significant economic impact for the producers.","palabras_clave":"FOSFOMYCIN|WITHDRAWAL TIME|PIGS|BROILER CHICKENS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56068,"idioma_id":4,"titulo":"ENFICA-FC: Design of Transport Aircraft Powered by Fuel Cell & Flight Test of Zero Emission 2-Seater Aircraft Powered By Fuel Cells fuelled by Hydrogen","resumen":"Fuel cells could become the main power source for small general aviation aircraft or could replace APU and internal sub-systems on larger aircraft, to obtain all-electric or more-electric air vehicles. There are several potential advantages of using such a power source, that range from environmental and economic issues to performance and operability aspects. A preliminary design is reported. Also, the paper contains a description of testing activities related to experimental flights of an all-electric general aviation aircraft fuelled by hydrogen. Great importance has been given to the testing phase of the prototype  and  examples of  each testing stage are shown ranging from the single components to the final test flights. During the 6 experimental flights a rotation speed of 84 km\/h was obtained in 184 m of taxi at power of 35 kW. Level flight was attained at 135 km\/h and endurance of 39 min. by mean of only a fuel cell power setting (speed world record for the FAI Sporting Code Category C - airplane).","palabras_clave":"Fuel cell airborne application|light test aircraft powered by fuel cell|Design transport aircraft powered by fuel cell"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56080,"idioma_id":5,"titulo":"Entre la Nación y la emancipación: la producción musical académica de las mujeres en Buenos Aires durante los conflictos políticos de 1945","resumen":"En septiembre de 1945, mientras la Argentina transitaba una fuerte crisis política e institucional bajo un gobierno militar de facto, los homenajes por el centenario de la muerte de Bernandino Rivadavia articularon una coyuntura para la movilización, la ocupación del espacio público y la agencia política de las mujeres. Distintos sectores de la política, el arte y la cultura le rindieron, al prócer, calurosos tributos en todo el país, incluido el Ejército. El Círculo Militar de Buenos Aires ofreció en su memoria una audición de obras de compositoras residentes en la ciudad. Este artículo se propone ahondar en la motivación ideológica y la significación política de este ?uso? que el campo de las fuerzas autoritarias hizo de estas mujeres y su música, y que ellas ocuparon simbólicamente con un repertorio representativo del nacionalismo musical.","palabras_clave":"COMPOSITORAS ARGENTINAS|MÚSICA Y POLÍTICA|NACIONALISMO MUSICAL|GÉNERO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56123,"idioma_id":5,"titulo":"UN LUGAR EN EL MUNDO","resumen":"La obsesión acerca de ?cómo nos ven afuera? está involuntariamente emparentada con el narcisismo de quien son-ríe en el momento del relámpago porque cree que Dios le toma una fotografía. En el discurso público más corriente, fuera de los círculos de especialistas, ésa tiende a ser la pregunta que organiza la conversación sobre la política exterior argentina. Lo ha sido al me-nos desde algún momento que podríamos situar convencionalmente alrededor de ese acontecimiento de cambio inevitable de la geopolítica que fue el &#64257;n de la Guerra Fría. El cambio en el orden mundial que signi&#64257;có el &#64257;n de la bipolaridad impactó sobre la política exterior de todos los países del mundo. En Argentina, ese cambio epocal coincidió con un viraje en la política doméstica que se puede de&#64257;nir como una adopción sui generis del programa implícito en el llamado Consenso de Washington. Ese programa presuponía, entre otras cosas, la adopción de políticas conducen-tes a un ?clima de negocios? enmarca-do en una creciente apertura comercial y favorable a la llegada de la inversión extranjera. El discurso político o&#64257;cial presentó ese marco de política pública como una puerta de acceso al ?primer mundo? y el debate sobre la política exterior ?siempre limitado y escasamente informado? fue puesto bajo ese prisma.","palabras_clave":"TIERRA |POSESION|IDENTIDAD|SUBJETIVIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56161,"idioma_id":5,"titulo":"Medidas de seguridad y derecho penal. El caso de Rodolfo desde una perspectiva antropológico","resumen":"El artículo da cuenta del conjunto de dispositivos penales que regulan, administran y aplican las medidas previstas para personas que en estado de inimputabilidad, son objeto de una reclusión penal distinta a la pena privativa de la libertad. No debe perderse de vista que dicho campo se encuentra inscripto en el entramado de un mundo signado por el ejercicio de una nueva gubernamentalidad neoliberal, que incluye al menos dos elementos destacables a tener en cuenta en este caso: el incremento mundial de la proporción de población en conflicto directo con la ley (acompañado por una fuerte política de construcción de prisiones a escala global y el aumento de los presupuestos estatales destinados a las fuerzas de ?la ley y el orden?); junto con una creciente ?autopropulsión? del miedo a nivel general, que deviene finalmente en una exacerbada preocupación por la protección personal (Bauman 1999). A través de una mirada antropológica del caso presentado, complementada con la lectura de la normativa pertinente, la observación de los procedimientos judiciales y la dinámica de funcionamiento de la unidad penitenciaria psiquiátrica involucrada, se considera que el sistema de medidas de seguridad curativas se desenvuelve como un verdadero régimen de excepción.","palabras_clave":"DERECHO PENAL|MEDIDAS DE SEGURIDAD|PELIGROSIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56165,"idioma_id":5,"titulo":"La construcción de la verdad jurídica: prueba, interpretaciones y disputas en torno a la administración de inimputabilidad. El caso del 'tirador serial'.","resumen":"Uno de los objetivos del proceso penal es averiguar si determinados hechos han ocurrido o no y fundamentalmente, en qué términos y circunstancias. En este sentido, la 'prueba' constituye un factor de conocimiento cuya función principal es establecer 'la verdad de los hechos'. Sin embargo, tanto el juzgamiento de la capacidad de culpabilidad como la valoración de la conducta de un imputado son en sí mismos procesos de interpretación, que requieren del juzgador una actitud activa y determinante. Desde una perspectiva antropológica, y a partir de un caso tomado como campo para la reflexión, el artículo intenta analizar la incidencia de valoraciones sociales, morales, éticas e ideológicas que intervienen en un proceso penal donde se discute la declaración de inimputabilidad del acusado; así como también dar cuenta de las influencias y disputas establecidas entre los diversos discursos ofrecidos en el desarrollo de la causa judicial.","palabras_clave":"discurso jurídico|verdad jurídica|inimputabilidad|sujeto inimputable"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56175,"idioma_id":5,"titulo":"Primer Estudio en Misiones de Genes de Resistencia a Macrólidos en Streptococcus agalactiae.","resumen":"Streptococcus agalactiae(SGB) es la primera causa de infecciones severas invasivas en el recién nacido. Penicilina es la droga de elección en la profilaxis intraparto que se realiza en la embarazada colonizada con SGB, pero en aquéllas alérgicas a los &#946;-lactámicos , eritromicina (ERI) y clindamicina (CLI) son las drogas de elección. Conocer los fenotipos de resistencia a ERI y CLI es crucial a la hora de prevenir esta severa enfermedad en los neonatos nacidos de gestantes colonizadas alérgicas a los &#946;-lactámicos. Se seleccionaron al azar 5 cepas con algún fenotipo de resistencia y cinco cepas sin ellos, mantenidas a -70 ºC,&nbsp;obtenidas de hisopados vagino-rectales de gestantes, entre los años 2004 y 2011. Los biotipos se determinaron con sueros del Statens Serum Institut Copenhagen, los fenotipos con test D-test, los&nbsp;genes ermB, ermTR y mef A con PCR . De las 5 cepas con fenotipo de resistencia, dos cepas fueron constitutivas, una inducible y dos fenotipos M. En los biotipos V y III se detectó el mayor número de genes de resistencia. El gen de resistencia más comunmente detectado fue el ermBseguido de mef A.&nbsp; Alguno de los genes de resistencia investigados fueron detectados en tres cepas sin fenotipo de resistencia. El conocer la asociación de biotipos, fenotipos y genes de resistencia a ERI y CLI, circulantes en la región, datos aún no existentes, colabora en la salud individual y colectiva en profilaxis y tratamientos y en el diseño de estrategias vacunales, aún en marcha.","palabras_clave":"Biotipos|Genotipos|Resistencia|Macrólidos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56188,"idioma_id":5,"titulo":"El riesgo a la libertad. La voz de las víctimas en las noticias televisivas","resumen":"A fines de 2009, las historias sobre Santiago Urbani inundaron los noticieros televisivos. El caso de un joven muerto tras sufrir un robo imputado a dos adolescentes de 16 y 17 años, fue jerarquizado en la cobertura de Telefé Noticias, América Noticias, Todo Noticias, Canal 5 Noticias y Visión 7. El objeto de este artículo es reflexionar sobre uno de los ejes de la tesis de grado 'Delito, juventudes y castigo. La construcción del caso Urbani en cinco noticieros de televisión', que analiza los modos en que las técnicas periodísticas de producción de temor dieron forma a los conflictos en el campo televisivo. La construcción de relatos victimizantes exhibió una ciudadanía perturbada, saturada, demandante, que clamó por seguridad y justicia en un contexto de presión hacia la reformulación de la legislación vigente en material penal juvenil. Este trabajo sintetiza algunos resultados en relación al problema de la generación de percepciones de riesgo hacia el micro-delincuente y su incidencia en los mecanismos que habilitaron la legitimación de prácticas punitivas en las noticias periodísticas.","palabras_clave":"DISCURSOS PERIODÍSTICOS|INSEGURIDAD|VICTIMIZACIÓN|JÓVENES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56192,"idioma_id":5,"titulo":"Comentario a Zygmunt Bauman. Daños colaterales. Desigualdades sociales en la era global. Fondo de Cultura Económica, Buenos Aires, 2011","resumen":"Daños colaterales es un concepto de origen bélico seleccionado por Bauman para criticar el modo en que se naturalizan los efectos del capitalismo global bajo el supuesto de que el proceso de creciente desigualdad no constituye en sí mismo un peligro para el orden social. La marginalidad despierta respuestas criminalizantes por razones similares a las que impiden que el sufrimiento de las clases pobres componga un tema de la agenda política. El deseo de no enfrentar el problema de la desigualdad por parte de los gobiernos y un sector de la ciudadanía ofusca, y acaso subestima, los aspectos estructurales de un ordenamiento que les comprende. La falta de percepción de la analogía intrínseca que conlleva la relación entre desigualdades y bajas colaterales obstruye ver la mayor amenaza a la que se enfrenta la humanidad durante este siglo. Tarde o temprano, quienes deciden sobre los beneficios de los riesgos descargados sobre sectores socialmente excluidos acabarán padeciendo las consecuencias.","palabras_clave":"DAÑOS COLATERALES|DESIGUALDADES|ERA GLOBAL|RIESGOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56272,"idioma_id":4,"titulo":"Assessment of Sperm Function Parameters and DNA Fragmentation in Ejaculated Alpaca Sperm (Lama Pacos) by Flow Cytometry.","resumen":"Flow cytometry has been shown to be an accurate and highly  reproducible tool for the analysis of sperm function. The main  objective of this study was to assess sperm function parameters  in ejaculated alpaca sperm by flow cytometry. Semen samples  were collected from six alpaca males and processed for flow  cytometric analysis of sperm viability and plasma membrane  integrity using SYBR-14&#8260;PI staining; acrosomal membrane  integrity using FITC-conjugated Pisum Sativum Agglutinin&#8260;PI  labelling; mitochondrial membrane potential (Dwm) by staining  with JC-1 and DNA Fragmentation Index (DFI) by  TUNEL. The results indicate that the mean value for sperm  viability was 57 ± 8 %. Spermatozoa with intact acrosome  membrane was 87.9 ± 5%, and viable sperm with intact  acrosomal membrane was 46.8 ± 9%, high mitochondrial  membrane potential (Dwm) was detected in 66.32 ± 9.51% of  spermatozoa and mean DFI value was 0.91 ± 0.9%. The DFI  was inversely correlated with high Dwm (p = 0.04; r = 0.41)  and with plasma membrane integrity (p = 0.01; r = 0.47). To  our knowledge, this is the first report of the assessment on the  same sample of several parameters of sperm function in  ejaculated alpaca sperm by flow cytometry.","palabras_clave":"ESPERMATOZOIDES|ALPACA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56276,"idioma_id":4,"titulo":"Infertility treatment, a matter of a lovely sperm?","resumen":"Nowadays, the efficiency of the infertility  treatment is relatively low. One of  the cues to counteract this problem relies on  the optimum selection of spermatozoa. We  developed a new method (sperm selection  assay (SSA)) based on the chemical attraction  of spermatozoa that are at the best  functional state. Additionally, the SSA leads  spermatozoa to complete and\/or acquire  the competence to fertilize the egg. These  effects are equally observed either in normal  or subfertile semen samples. Those capabilities  of SSA may improve the success of  current infertility treatment.","palabras_clave":"ESPERMATOZOIDES|PROGESTERONA|QUIMIOTAXIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56286,"idioma_id":5,"titulo":"Para una historia de las lecturas del género policial en América Latina","resumen":"El artículo se propone, a partir de la lectura de un conjunto de ensayos de escritores y críticos de diversos países de Latinoamérica, historizar los modos en que fue pensado el género policial en América Latina desde las vanguardias hasta la primera década del siglo XXI.\tDe esa lectura surge una historia, que puede pensarse a partir de dos umbrales. El primero tiene lugar alrededor de la década del cuarenta y es el resultado de la articulación, a veces incómoda, entre la herencia de las vanguardias y un renovado horizonte de legibilidad para el relato policial. El segundo umbral, que comporta una redefinición de los valores y los referentes de la crítica del género, tiene lugar alrededor de la década del setenta. A partir de ese momento se abren dos líneas de reflexión que, aun teniendo puntos de contacto, resultan complementarias en su atención a las posibilidades de variación del género.","palabras_clave":"Policial|Latinoamérica|Género|Historia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56290,"idioma_id":5,"titulo":"Gestos del enigma: Los misterios de la ópera","resumen":".. En Latinoamérica se el género policial de enigma puede rastrearse con modalidades diversas pero relativamente canónicas desde fines del siglo XIX. Durante el siglo XX el género se consolida en los mercados literarios en expansión entre fines de la década del treinta y los sesenta. Hacia la década del setenta comienza un proceso de transformación del género cuyas características permiten postular la emergencia de una nueva articulación entre Estado, verdad, relato y ley. En esa nueva articulación la vertiente de enigma pierde presencia, marginada por su  irrealismo, identificado, a partir de los sesenta, con ?banalidad?  ...Los últimos quince años, sin embargo, han visto un renovado ineterés por el policial de enigma, como prueban novelas de Guillermo Martínez, Pablo De Santis, Rubem Fonseca o Rodrigo Rey Rosa. Este trabajo se centra en los relatos del mexicano Emmanuel Matta incluidos en Los misterios de La Ópera y estudia los modos en los que el policial de enigma es retomado. En particular se presta atención al modo en que la resolución del enigma se presenta de modo diferente tanto con respecto a las formulaciones clásicas del relato policial como con respecto a las formulaciones características del relato policial en América Latina.","palabras_clave":"Género policial|Literatura Contemporánea|Literatura Mexicana|Emmanuel Matta"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56297,"idioma_id":5,"titulo":"Para llegar a McOndo","resumen":"Durante la década del noventa se realizaron diversos diagnósticos sobre lo que se percibía como un nuevo momento de la narrativa latinoamericana. Esos discursos suelen considerar la condición ?negativa?, carente, de esas narrativas. Este trabajo pretende empezar a diseñar otras variantes que puedan describir el período, a partir de la lectura del libro McOndo, la antología preparada por Alberto Fuguet y Sergio Gómez en 1996.","palabras_clave":"McOndo|Literatura latinoamericana|Literatura contemporánea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56302,"idioma_id":5,"titulo":"El programa y el estatuo. Sobre Colabor-arte, de Mario Carlón y Carlos Scolari","resumen":"Reseña del libroi de Mario Carlón y Carlos Scolari, 'Colabor-arte', sobre los nuevos modos de prácticas colaborativas en Internet..","palabras_clave":"Nuevos medios|Nuevas estéticas|Prácticas colaborativas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56316,"idioma_id":5,"titulo":"'Entropía, esquizofrenia y pérdida del principio de realidad. A propósito del sincretismo psicoanálisis- termodinámica en Philip K. Dick'","resumen":"El presente trabajo aborda la experiencia de lo siniestro y su relación con los conceptos de entropía, esquizofrenia y realidad (tan presentes en la narrativa dickiana) a partir del análisis de la novela 'Martian Time- Slip'. El objetivo es exponer las implicancias de la experiencia de lo siniestro para el sujeto, el cual se encuentra siempre tensionado entre la degradación entrópica del 'Koinos Kosmos' que deviene con la psicosis y la reproducción maquinal y neurótica del ciclo cultural.","palabras_clave":"PHILIP K. DICK|ESQUIZOFRENIA|TERMODINÁMICA| PSICOANÁLISIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56589,"idioma_id":5,"titulo":"Notas para combatir el combate a la pobreza","resumen":"El presente trabajo se propone poner en discusión una serie de postulados centrales de la Gerencia Social, en particular la idea de combate contra la pobreza, que ha permeado durante las últimas décadas los debates en torno de la asistencia social. Toma como punto de partida el paradigma del desarrollo humano y el enfoque de las capacidades (HDCA - Human Development and Capability Approach) para mostrar cómo a esa perspectiva la Gerencia Social le sobreimprime elementos tanto de carácter moral como pseudo-científicos. Para ello, realiza un relevamiento documental y bibliográfico que da cuenta de los principales nudos teórico-epistemológicos tanto del HDCA como de la Gerencia Social, haciendo especial énfasis en los efectos de ambas perspectivas sobre la concepción de la pobreza y, por ende, también de la política social. La apuesta es contribuir a un análisis en términos de gobierno de la pobreza, que entiende a esta última como una construcción socio-histórica producto de una determinada problematización, más que como un flagelo o mal inevitable que hay que combatir.","palabras_clave":"POBREZA|DESARROLLO HUMANO|GERENCIA SOCIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56601,"idioma_id":5,"titulo":"Pinzas ópticas y su aplicación en biología","resumen":"La técnica de pinzas ópticas (también llamadas trampasópticas o láser) combinadas con técnicas de seguimientode partícula única permiten manipular objetos en laescala nanométrica y determinar fuerzas del orden delpico Newton. Dado que los sistemas biológicos seorganizan con esa precisión espacial mediante fuerzas deinteracción de dicho orden de magnitud, la metodologíapermite interrumpir o imitar procesos biológicos y asícomprender los mecanismos y los principios que losrigen.La trampa se genera en un punto en el espacio en el queun haz láser es enfocado, debido a la interacción de laradiación electromagnética con la partícula a atrapar.Diferentes diseños experimentales han aportado valiosainformación relacionada con el funcionamiento y laenergética de procesos realizados por motoresmoleculares, el plegamiento de diversas macromoléculasasí como la plasticidad de la membrana celular.Aquí se resumirán los fundamentos y montajes de latécnica y sus posibles aplicaciones en biología.","palabras_clave":"PINZAS OPTICAS|ESTIRAMIENTO DE MACROMOLÉCULAS|REOLOGÍA DE MEMBRANAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56605,"idioma_id":4,"titulo":"Skull geometric morphometrics and paleoecology of santacrucian (late early Miocene; Patagonia) native ungulates (Astrapotheria, Litopterna, and Notoungulata)","resumen":"Three orders of South American extinct native  ungulates are recorded from the Santa Cruz Formation along the Atlantic coast  of Patagonia: Notoungulata (Adinotherium Ameghino,  Nesodon Owen, Interatherium Ameghino, Protypotherium  Ameghino, Hegetotherium Ameghino, and  Pachyrukhos Ameghino), Litopterna (Theosodon Ameghino, Anisolophus Burmeister, Tetramerorhinus  Ameghino, Diadiaphorus Ameghino, and Thoatherium Ameghino), and Astrapotheria  (Astrapotherium Burmeister). An ecomorphological study based on geometric  morphometrics of the masticatory apparatus was performed. The reference sample  included 618 extant specimens of the orders Artiodactyla, Perissodactyla,  Hyracoidea, and Diprotodontia. Thirty six cranial and 27 mandibular  three-dimensional landmarks were digitized. Allometric scaling, principal  component analyses, and phylogenetic generalized estimating equations on the  cranium and mandible were preformed. Analyses of cranial shape show strong  phylogenetic constraints, whereas the mandibular analyses show a functional  pattern related to habitat\/diet and hypsodonty. The extant brachydont ungulates  from closed habitats show a more elongated and narrower mandibular symphysis  with a lower mandibular corpus, than hypsodont, open habitat species. The  latter have short symphyses with a high, curved mandibular corpus. This morphological  pattern was also present among Santacrucian ungulates, allowing  characterization of notoungulates mainly as open habitats dwellers, with some taxa  foraging on grass (Protypotherium,  Interatherium), and others on grass and leaves (Hegetotherium, Pachyrukhos,  and Adinotherium), depending  on the availability. Nesodon  may have dwelled in mixed habitats and had a mixed feeding behavior, while  small proterotheriids (Anisolophus  and Thoatherium) may have fed  predominantly on dicotyledonous plants. The remaining  litopterns (Tetramerorhinus, Diadiaphorus, and Theosodon) and  Astrapotherium may have foraged in closed habitats and fed on  dicotyledonous plants.","palabras_clave":"Ecomorphology|Masticatory apparatus|Skull allometry|Habitat|Diet|Paleobiology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56662,"idioma_id":5,"titulo":"ECONOMÍA Y ANTROPOLOGÍA, DIÁLOGOS DISCIPLINARES","resumen":"El siguiente articulo supone un recorrido en la historia del debate entre Antropología y Economía. En esta primera instancia se observa el período fundacional de la disciplina Antropológica,  recorriendo someramente los primeros trabajos de corte evolucionista para luego adentrarse con mayor detalles en la labor de quienes fundaran la Antropología económica como rama disciplinar. Se sintetizan las principales hipótesis de los estudios de Malinowski respecto del Kula y de Mauss en relación a los sistemas de intercambio y la aparición del don como categoría. Es el objetivo de este trabajo exponer las diversas forma de comprensión de la propiedad, y la riqueza existentes en otras comunidades y de cómo la disciplina antropológica se esforzó en problematizar cuestiones relativas a la economía en contextos donde las categorías occidentales no podían extrapolarse.","palabras_clave":"ANTROPOLOGIA|ECONOMIA|HISTORIA|CONCEPTUALIZACIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56666,"idioma_id":4,"titulo":"WHY ARE THEY CONFINED? THE KNOWLEDGE AND PRACTICES OF INSTITUTIONALISED CHILDREN","resumen":"The construction of the notion of childhood as an historical phenomenon has provoked the development of afield of studies that is located at a disciplinary crossroads, of psychology, sociology, history and anthropology.This field of work supposes a transdisciplinary construction of an object-subject \u2013 an object that is studied, butwhich is also the subject of its own activity \u2013 and it is not studied separately but within a system of relationshipsthat it maintains with others. Social context assigns boys and girls different roles: for example, as a child, asyoungster, as offender, or as consumerIn Latin America, and particularly in Argentina, the background of exclusion and enlarging poverty, madechildren socially vulnerable, and confined to institutions.We describe results from a piece of qualitative research that concern \u2018social representations\u2019 utilised by boysand girls who live in private homes who are run under the auspices of government organizations in BuenosAires city. We illustrate the way in which these institutionalisation processes affects the identity creation.","palabras_clave":"CHILDREN|SOCIALVULNERABILITY|INSTITUTIONALIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":56993,"idioma_id":4,"titulo":"Heat Transfer Properties of a Series of Oxidized and Unoxidized Vegetable Oils in Comparison with Petroleum Oil-Based Quenchants.","resumen":"Vegetable oils, especially soybean oil, exhibit substantially poorer thermal-oxidative stability than commercially available petroleum oil quenchant formulations. Therefore, to achieve any commercially interesting performance vegetable oils must be stabilized by the addition of antioxidant inhibitors. This work describes the ability of two commercially available antioxidants, Irganox L 57 and Irganox L 109 to stabilize soybean oil against thermal-oxidative degradation. In addition, the effect of antioxidant stabilization on quenching performance was evaluated by determining the profile of heat transfer coefficient variation throughout the quenching process at different times after being subjected to an accelerated thermal-oxidation ageing test. The results of this work are discussed here.","palabras_clave":"Vegetable oils|Antioxidants|Quenchants|Cooling Curve Analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57133,"idioma_id":4,"titulo":"Error estimation in environmental DNA targets quantification due to PCR efficiencies differences between real samples and standards","resumen":"In real-time PCR the amplification and subsequent fluorescence detection of a genetic target occurs simultaneously during each cycle. PCR efficiency is driven by the amplification process; theory indicates that DNA duplicates in each cycle, but different factors which are related to reagents performance, methodological procedure and sample DNA quality, frequently have a considerable impact on DNA amplification. Absolute quantification is achieved using a standard curve constructed by amplifying known amounts of a target DNA; being the current approach utilized in environmental microbiology. Nevertheless, PCR efficiency obtained using good quality standard DNAs from pure cultures, plasmids, or cDNA, may be different than that obtained using DNA from environmental samples. This fact contributes to an analytical bias and consequently introduces a quantification error. In the present work, we have addressed this problem by analyzing a large number of environmental samples for the detection of Bacteroides spp., Legionella pneumophila or Adenovirus spp. We demonstrated the importance of calculating the PCR efficiency for both, standard and sample DNAs, in order to correctly quantify the amount of microbial target in environmental samples. We noted that environmental samples with low concentration of target DNA significantly differ in the PCR performance from that calculated using standards, leading to substantial bias in the quantified DNA concentration. Therefore, the results herein showed, point out the paramount importance of knowing the PCR efficiency for each procedure and every particular kind of environmental sample in order to optimize the complete workflow or, at least, to correct the final results with an appropriate factor. ","palabras_clave":"REAL-TIME PCR|ENVIRONMENTAL DNA SAMPLE|PCR EFFICIENCY|QUANTIFICATION ERROR ESTIMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57145,"idioma_id":5,"titulo":"Fuera de quicio. Bolaño en el tiempo de sus espectros, Raul Rodriguez Freire, editor","resumen":"Reseña del volumen crítico sobre Bolaño. A diez años de la muerte de Roberto Bolaño lecturas críticas de su obra proliferan en  diferentes formatos y medios (artículos, blogs, conferencias, coloquios, libros, etc.). ¿Cómo  abrirse camino ante el desaforado aluvión de comentarios, análisis y lugares comunes? Con  este panorama, Raúl Rodríguez Freire compila un nuevo volumen crítico sobre el escritor de  Los detectives salvajes y, reconociendo sólo dos antecesores (los trabajos iniciales de Celina  Manzoni y Patricia Espinosa), asevera el cambio de época respecto de la recepción de la obra  de Bolaño. Los pioneros trabajos hacen eco de la urgencia de leer y mostrar, mientras que  finalizando la primera década del nuevo siglo el proyecto de Rodríguez Freire se presenta  ?contra su banalización y el consecuente extravío de su potencia?","palabras_clave":"BOLAÑO|CRÍTICA LITERARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57283,"idioma_id":5,"titulo":"Crónica: V Jornadas de Estudios Clásicos. Diálogos Culturales","resumen":"V Jornadas de estudios clásicos y medievales ?diálogos culturales?  la plata, universidad nacional de la plata, 5 a 7 de octubre de 2011  Entre los días 5 y 7 de octubre del 2011 tuvieron lugar en la ciudad de La   PlatalasVJornadasdeEstudiosClásicosyMedievales,?DiálogosCulturales?,  organizadas por el Centro de Estudios Latinos y el Centro de Estudios de Teoría   y Crítica Literarias (Literatura Espeñola Medieval), UNLP, FaHCE, IdIHCS.  Las Jornadas estuvieron divididas en conferencias, paneles, comisiones   de lectura sobre distintos temas de la Antigüedad Clásica y la Edad Media, y   tuvimos el agrado de contar con la presencia del Dr. Gómez Pallarès (Universidad   Autónoma de Barcelona) quien estuvo a cargo del Curso Internacional 2011 del","palabras_clave":"JORNADAS|DIÁLOGOS CULTURALES|LA PLATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57473,"idioma_id":5,"titulo":"Poesía argentina de los noventa: escrituras artesanales","resumen":"A finales de los noventa, en Argentina,  emergió un   grupo de proyectos editoriales, conocidos bajo distintos tipos de    nombres como editoriales independientes, alternativas o artesanales.   Estos  proyectos constituyen lo que llamamos, aquí, un ?dispositivo de   escritura  independiente?: proyectos donde las prácticas poéticas están   íntimamente  relacionadas con las prácticas de edición. Por esta razón,   desarrollaremos la  idea de ?escrituras artesanales? que define este   corpus de textos poéticos y  contribuye en la instauración de una nueva   política de colaboración y trabajo  grupal.","palabras_clave":"Poesía argentina de los noventa|Editoriales independientes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57477,"idioma_id":5,"titulo":"La edición como límite de la literatura Aproximaciones al catálogo de Belleza y Felicidad","resumen":"El siguiente trabajo analiza las  escrituras reunidas en   torno a Belleza y Felicidad, atendiendo a las  proximidades entre   poética y edición, a las conexiones entre materialidad y  prácticas de   escritura. Los libros de Belleza y Felicidad son fotocopias dobladas  y   abrochadas por la mitad, con tapas dibujadas a mano. Su particular    disposición material está en diálogo con los textos del catálogo, hasta   el  punto que esa misma práctica de edición instala una estética   definida que  involucra y modifica la idea de literatura que encontramos   en este proyecto.","palabras_clave":"Poesía argentina de los noventa|Editoriales Independientes|Belleza y Felicidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57558,"idioma_id":5,"titulo":"Algunas consideraciones en la investigación contable a lo largo de la historia. Consecuencias de la regulación.","resumen":"El presente trabajo constituye una síntesis de planteamientos tradicionales en la investigación contable, tomado desde los autores de Europa Continentalmente fines del siglo XIX y principios del siglo XX, el hacendalismo y el patrimonialismo en particular, para llegar a Cañibano y los programas de investigación en contabilidad y Tua Pereda con los sub-programas de investigación.La idea central el ver como la Contabilidad ha sido flexible a los cambios económicos, políticos y sociales y cómo la regulación contable ha acompañado ese proceso.","palabras_clave":"Autores de Europa continental|Programas de investigación en contabilidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57587,"idioma_id":5,"titulo":"Reseña de una tesis sobre la Plástica en la escuela. La educación artística y su relación con los derechos de niños, niñas y adolescentes","resumen":"En este artículo presento una síntesis de la tesis que elaboré en el marco del Doctorado en Artes de la Facultad de Bellas Artes de La Plata, que se titula: ?La educación artística: paradigmas y saberes en el aula? y fue dirigida por la Dra. Leticia Muñoz Cobeñas.La temática que abordo en ella proviene de dos investigaciones que realicé como becaria de la Universidad Nacional de La Plata. En ambas investigaciones estudié las prácticas de enseñanza-aprendizaje de la Plástica en escuelas secundarias estatales de La Plata. Las motivaciones de la elección de este tema las encuentro en mi experiencia previa como profesora de Plástica, a partir de la cual empecé a interrogarme sobre el sentido de la educación artística en la institución escolar. Adoptando la perspectiva paradigmática de la promoción y protección de derechos de niños, niñas y adolescentes.","palabras_clave":"ARTES PLÁSTICAS|EDUCACIÓN|SABERES|PRÁCTICAS|DERECHOS DE NIÑOS, NIÑAS Y ADOLESCENTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57588,"idioma_id":5,"titulo":"El lenguaje y la construcción de sentido. La producción y la interpretación de imágenes.","resumen":"La enseñanza y el aprendizaje relacionados con la producción visual permiten el trabajo sobre sistemas de signos para la construcción poética. Las imágenes plásticas son susceptibles de ser abordadas como indagaciones sobre el lenguaje, sobre sus posibilidades semánticas. Y pueden ser también herramientas para dar testimonio sobre la cultura contemporánea.","palabras_clave":"EDUCACIÓN ARTÍSTICA|CULTURA CONTEMPORÁNEA|SEMIÓTICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57651,"idioma_id":5,"titulo":"Especias y condimentos empleados en la cocina andina tradicional","resumen":"Los productos que emplea la cocinatradicional andina para saborizar y condimentar sus platos se comercializan enmercados y ferias regionales, llegando en ocasiones a las herboristerías. Conel propósito de conocer la calidad botánica de estos productos de origen andinoque se comercializan en la ciudad de S. S. de Jujuy y alrededores se aborda suestudio mediante el método micrográfico.Se aplica el patrón de identificaciónmicrográfico a productos comerciales elaborados con cuatro especies: Acantholippiasalsoloides, ?rica rica?, Verbenaceae, Clinopodium gilliesii, ?muñamuña?, Lamiaceae y Senecio nutans, ?chachacoma? y Xenophyllum poposum,?pupusa?, Asteraceae empleando la técnica de disociado leve (tratamiento delmaterial con NaOH durante 5 min. a ebullición) y con diez repeticiones paracada muestra comercial.El examen macro y microscópico de losmateriales revela que solamente ?rica rica? demuestra ser un producto genuino,mientras que los restantes presentan contaminaciones con otras especiesvegetales.Estos datos indican la importancia de laaplicación de la micrografía como estrategia metodológica para llevar a cabo elcontrol de calidad botánico de productos destinados al consumo humano; se tratade técnicas sencillas y rápidas que ofrecen resultados sustentados eninformación científica como son los identificadores previamente establecidospara las especies estudiadas.","palabras_clave":"MICROGRAFÍA|ALIMENTOS ANDINOS|CALIDAD BOTÁNICA|MUÑA MUÑA|RICA RICA|CHACHACOMA|PUPUSA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57748,"idioma_id":5,"titulo":"Las competencias comunicativas  en estudiantes universitarios  que cursan en Sistemas  de Aprendizaje Distribuido (SAM) con modalidad Blended Learning?","resumen":"En esta ponencia se presentan los resultados de una investigación llevada a cabo en el año 2012 en el marco de la Maestría en Tecnología Educativa del TEC de Monterrey, México. En dicha investigación se indagó sobre las competencias comunicativas, como posibilidades de acción vinculadas a los aprendizajes en los entornos de enseñanza Blended Learning. Esta inquietud surgió en virtud de que actualmente existen nuevos escenarios comunicativos en instituciones educativas que han incorporado a su oferta educativa la modalidad a distancia. En este sentido el  tema de investigación remitió a la problemática referida a las competencias comunicativas en alumnos de una universidad argentina, en la cual cursan una Licenciatura en Educación a través de sistemas multimediales, combinando aprendizaje presencial y virtual (b- learning). La investigación fue realizada desde un enfoque mixto, combinando la metodología cuantitativa con la cualitativa. La población estudiada estuvo compuesta por 30 estudiantes. Los instrumentos de recolección de datos fueron encuestas con preguntas cerradas y abiertas, entrevistas y seguimiento de intercambios en la plataforma virtual. El trabajo, en líneas generales,  permitió conocer lo que acontecía con las competencias comunicativas en propuestas Blended Learning en las cuales las prácticas de la enseñanza suponen   procesos de aprendizaje relativos a lo conceptual, a lo tecnológico y a lo comunicativo, como desempeños que se enmarcan en nuevas formas de enseñanza.  Los resultados abordan las  competencias comunicativas previas como así también las competencias actuales puestas en juego por los estudiantes en relación a las tecnologías de la información y la comunicación, las comunicaciones virtuales entre alumnos y  docente tutor, los aspectos favorecedores y obstaculizadores del sistema multimedial en lo que refiere a las competencias comunicativas, a la construcción de conocimiento y a las modalidades interactivas que se dan en los espacios presenciales y en los espacios virtuales.","palabras_clave":"modalidad distribuida|educación a distancia|modalidades combinadas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57759,"idioma_id":5,"titulo":"Pensamiento complejo en relaciones internacionales: la cuestión de la seguridad","resumen":"El pensamiento complejo como posibilidad epistemológica en Relaciones Internacionales, aparecerá con el fin de la Guerra Fría y los cambios mundiales producidos por la globalización, como una necesidad por encontrar nuevas herramientas que dieran cuenta de los cambios producidos a nivel mundial. De la preponderancia de un pensamiento lineal, racionalista y que se pretendía objetivo se pasó a un profundo debate disciplinar sobre la urgencia de incorporar otras miradas, que vincularan la comprensión de los fenómenos internacionales más que la explicación o la previsión y que reconocieran la complejidad de la realidad social global a la par de la multidimensionalidad de los fenómenos implicados en ella.Ha sido la cuestión de la Seguridad un eje disparador del debate, dado que gran parte de los cambios producidos en el mundo cuestionaban el pensamiento tradicional en torno a la Defensa y el rol del Estado como objeto de la misma. La Seguridad Humana o la concepción de Seguridad como Multidimensional generaron nuevas propuestas de abordaje que trascendían el uso de la fuerza para incorporar instrumentos de acción tales como: la prevención frente a amenazas globales y los conflictos, la cooperación antes que la confrontación, la extensión y profundización de la democracia. Sin embargo, los países centrales y en especial EE.UU como primera potencia militar mundial; se apropiaron, para su accionar, de las nuevas perspectivas sobre Seguridad reduciendo sus elementos constitutivos, al enfatizar el uso de la fuerza. Pudiendo justificarse a partir de las nuevas necesidades en Seguridad una serie de intervenciones a otros territorios que han implicado en ciertos casos violaciones a los derechos fundamentales (Kosovo, Irak, Afganistán). Entendemos desde el pensamiento complejo que existe una interacción inseparable entre teoría y praxis y por lo tanto, se trabajará en un ida y vuelta entre las visiones teóricas hegemónicas y las prácticas de las potencias.Este trabajo plantea que es necesario  sostener en el debate de las Relaciones Internacionales perspectivas que incorporen el pensamiento complejo y con ello el reconocimiento de la diversidad y la legitimidad del Otro como modo de encontrar posibilidades para una reflexión diferente sobre la realidad internacional.","palabras_clave":"pensamiento complejo|realismo|relaciones internacionales|seguridad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57769,"idioma_id":4,"titulo":"Residence place as a risk factor in different types of fatal car accidents","resumen":"The association between place of residence, population density, relief and type of event (collision or non-collision of the vehicle) has not been evaluated in developing countries. The main objective of this study is to determine the differential factors associated with the occurrence of deaths of collision and non-collision automobile users in Patagonia, Argentina. A multiple logistic regression analysis was performed using as the dependent variable death by car accident (collision or non-collision of the vehicle) and sex, age, place of residence of the victim, relief and population density as the independent variables. Collision fatalities were related to areas of high population density, while non-collision fatalities were related to areas of low population density, mountainous landscape and place of residence of the victims outside the Patagonian region. The results obtained in this study indicate the need to develop differential primary prevention policies by place of residence of car occupants, focusing on Patagonia non-resident drivers and by emphasising non-collision accidents.","palabras_clave":"PLACE OF RESIDENCE|POPULATION DENSITY|RELIEF|COLLISION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57854,"idioma_id":5,"titulo":"Viabilidad de mediciones de alcohol online en la Universidad  Nacional de Mar del Plata","resumen":"Las mediciones en psicología se han modificado debido a las nuevas herramientas que ofrece la tecnología. En estudiantes universitarios, la Web podría resultar una forma de evaluar conductas de riesgo como el consumo de alcohol. El objetivo de este trabajo es presentar la información disponible sobre la medición Web de consumo de alcohol y problemas relacionados en estudiantes universitarios y plantear una estrategia de aplicación en el medio local. Para ello, se realizaron búsquedas hasta el 28 de mayo de 2013 en las bases de datos MEDLINE, PsyINFO y ERIC. Se encontraron 235 artículos, de los cuales 27 fueron incluidos en la revisión luego de ser revisados a texto completo. Los estudios tenían menos de 10 años, eran principalmente descriptivos y realizados en países de habla inglesa. Para la selección de la muestra se utilizaron diversos medios de reclutación y se ofrecieron incentivos para la participación. Los cuestionarios más utilizados fueron el AUDIT, RAPI y DDQ. Los retos y cuestiones a tener en cuenta, así como la propuesta de aplicación en la Universidad Nacional de Mar del Plata, y las limitaciones de la información presentada son discutidos","palabras_clave":"CONSUMO DE ALCOHOL|PROBLEMAS RELACIONADOS CON EL USO DE ALCOHOL|ESTUDIANTES UNIVERSITARIOS|REVISIÓN BIBLIOGRAFICA|INTERNET"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":57999,"idioma_id":5,"titulo":"Rememorar en los espacios museales: de la contemplación a la experiencia","resumen":"En las últimas décadas el devenir museográfico ha registrado un llamativo  cambio a partir de la inclusión de nuevos usos y fórmulas de presentación  y escenificación, bajo la influencia de las actuales corrientes artísticas. El  presente artículo se propone estudiar las estrategias implicadas en la creación,  presentación y funcionamiento del museo de patrimonio histórico Museo  Taller Ferrowhite ?ubicado en Ingeniero White, puerto de Bahía Blanca,  provincia de Buenos Aires, Argentina? para analizar la modalidad dis- cursiva y los recursos puestos en juego al momento de contar la historia a la  cual dicho espacio remite. No se trata de un museo de arte sino de historia  cultural, pero dicha institución recurre a los más diversos recursos artísticos,  abriendo un campo ficcional original y dando por resultado un proyecto en el  que múltiples factores concurren para dotarlo de una capacidad comunicati- va ejemplar. En tal sentido, nuestro interés en analizar este espacio museal  se debe a las estrategias que este implementa, las cuales instituyen formas  de trabajo con la memoria comunitaria ?a nuestro juicio novedosas? que  plantean la necesidad de que la comunidad no asista a sus salas con una  actitud contemplativa sino predispuesta a la experiencia de rememorar","palabras_clave":"MUSEOS|MEMORIA|COMUNIDAD|ARTE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58008,"idioma_id":5,"titulo":"Arte y ciencia: convergencias en el marco de la teoría de la complejidad","resumen":"Teniendo en cuenta lo que ha ido aconteciendo en el arte y en la ciencia desde la creación de instituciones como el MIT Media Lab (1985) y las posteriores experiencias de artistas como Eduardo Kac o Stelarc durante la década del noventa, junto con el desarrollo del sci-art, las neurohumanidades y la proliferación de laboratorios de bioarte a comienzos del siglo xxi en todo el mundo, me propongo analizar el reconocimiento de la aleatoriedad del método científico como elemento unificador de dos esferas de conocimiento ?el arte y la ciencia? que, a partir de las experiencias mencionadas, conviven en un mismo espacio de producción: el laboratorio.  Como afirmaba Edgar Morin, «un paradigma es invisible». Quizás estas modalidades interdisciplinarias están abonando la formación de una tercera cultura y todavía estamos muy ciegos para darnos cuenta.","palabras_clave":"arte|ciencia|método|azar"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58011,"idioma_id":5,"titulo":"Estudio de las modificaciones del agua de los equipos odontológicos con salida a las salivaderas dentales","resumen":"El objetivo principal de esta investigación fue estudiar las modificaciones que sufre la flora planctónica de las tuberías metálicas de las unidades dentales, y el desarrollo de biofilm en el interior de los ductos con salida hacia las salivaderas, de 59 equipos odontológicos correspondientes a dos zonas diferentes del casco urbano de la ciudad de La Plata.","palabras_clave":"AGUA DE SALIDA|EQUIPOS ODONTOLOGICOS|SALIVADERAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58045,"idioma_id":5,"titulo":"Inocencio Aguado y Felipe Boero: ¿por qué ese otro?  El Himno a Sarmiento en disputa","resumen":"Se analizan los significados, valores estéticos y el sentido político atribuidos a dos Himnos a Domingo Faustino Sarmiento compuestos intencionalmente para las conmemoraciones del Cincuentenario del fallecimiento del prócer en 1938. Felipe Boero, impulsor del nacionalismo musical, compuso el Himno a Sarmiento sobre poesía de Eduardo María de Ocampo por encargo del Consejo Nacional de Educación. Inocencio Aguado Aguirre puso música al poema de Juan Solano Luis, Himno a Sarmiento en el Cincuentenario de su fallecimiento, por pedido del Consejo General de Educación de San Juan. Asimismo se analiza la omisión del Himno de Ocampo-Boero en los actos en San Juan, localidad natal del homenajeado, que protagonizó polémicas entre los músicos vinculados a los ámbitos de la Nación o de la Provincia. El conflicto demostró las pugnas por cuestiones políticas y de hegemonía cultural.","palabras_clave":"HIMNOS|LOCAL|NACIONAL|ALTERIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58049,"idioma_id":5,"titulo":"DEFINICIONES Y AYUDAS METODOLÓGICAS PARA UNA HISTORIA LOCAL DE LA MÚSICA","resumen":"Se reflexiona acerca de los problemas teóricos y prácticos que surgen al indagar en temáticas histórico musicales locales. Se analiza la aplicación de nuevos enfoques y propuestas metodológicas en investigaciones recientes, tomados en préstamo de la ?nueva historia cultural? y de la microhistoria en sus versiones italiana, española y mejicana. Asimismo se indaga la  factibilidad del diálogo y la circularidad entre estudios históricos de dimensión local, regional, nacional y global de la música.Versión electrónica:http:\/\/bibliotecavirtual.unl.edu.ar\/publicaciones\/index.php\/ISM\/issue\/current, ISSN: 2362-3322","palabras_clave":"MUSICOLOGÍA|EPISTEMOLOGÍA|LOCAL|METODOLOGÍAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58147,"idioma_id":5,"titulo":"El Estado en cuestión Reflexiones sobre el rol estatal en el neoliberalismo y el postneoliberalismo","resumen":"A partir del año 2005, el Gobierno de&nbsp; la Provincia de Buenos Aires había convocado a organizaciones&nbsp; sociales&nbsp; cercanas&nbsp; al&nbsp; gobierno&nbsp; nacional&nbsp; a&nbsp; integrar&nbsp; diferentes&nbsp; espacios de gestión gubernamental. El&nbsp; \u201Cmilitar\u201D el Estado o&nbsp; transformar&nbsp; la&nbsp; respuesta del Estado se convirtieron en objetivos primeros de&nbsp; las organizaciones. El&nbsp; interés de abordaje en este trabajo reside en reflexionar sobre la metáfora espacial de \u201Cacercar \u201Cel Estado a la ciudadanía, en particular a un grupo de organizaciones sociales fundadas al calor de la protesta y la resistencia al neoliberalismo, cuyo proceso de institucionalización interpeló fuertemente el debate en&nbsp; torno a que es el Estado, y que&nbsp; tipo de&nbsp; transformación de&nbsp; la respuesta estatal se podía generar al gestionar. Por&nbsp; ello&nbsp; abordaremos&nbsp; las&nbsp; transformaciones&nbsp; acaecidas&nbsp; durante&nbsp; el&nbsp; neoliberalismo&nbsp; que eclipsaron la figura del Estado, su autoridad y su representación, en base a analizar la racionalidad y prácticas que&nbsp; lo sustentaron, para posteriormente analizar&nbsp; las&nbsp; rupturas manifiestas en el proceso iniciado en el 2003, con la intención de concluir con una serie de dilemas presentes en este escenario.&nbsp;","palabras_clave":"ESTADO|NEOLIBERALISMO|POSNEOLIBERALISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58151,"idioma_id":4,"titulo":"Attending to warning signs of primary immunodeficiencies disease across the range of clinical practices","resumen":"PURPOSE:Patients with primary immunodeficiency diseases (PIDD) may present with recurrent infections affecting different organs, organ-specific inflammation\/autoimmunity, and also increased cancer risk, particularly hematopoietic malignancies. The diversity of PIDD and the wide age range over which these clinical occurrences become apparent often make the identification of patients difficult for physicians other than immunologists. The aim of this report is to develop a tool for educative programs targeted to specialists and applied by clinical immunologists.METHODS:Considering the data from national surveys and clinical reports of experiences with specific PIDD patients, an evidence-based list of symptoms, signs, and corresponding laboratory tests were elaborated to help physicians other than immunologists look for PIDD.RESULTS:Tables including main clinical manifestations, restricted immunological evaluation, and possible related diagnosis were organized for general practitioners and 5 specialties. Tables include information on specific warning signs of PIDD for pulmonologists, gastroenterologists, dermatologists, hematologists, and infectious disease specialists.CONCLUSIONS:This report provides clinical immunologists with an instrument they can use to introduce specialists in other areas of medicine to the warning signs of PIDD and increase early diagnosis. Educational programs should be developed attending the needs of each specialty.","palabras_clave":"primary immunodeficiencies|recurrent infection|cancer|autoimmunity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58217,"idioma_id":4,"titulo":"BACTERICIDAL SECONDARY METABOLITES PRODUCE BY Fusarium sp. AGAINST Staphylococcus aureus","resumen":"Entomopathogenic fungus (EF) causes the death of the host insect. EF are a rich source of natural bioactive compounds. When the insect dies several microorganisms that grow normally in the exoskeleton surface, as well as, in nearly all the epithelium, such as Staphylococcus aureus try to degrade the insect body. However, the EF consumes the insect. The objective was determined if the EF Fusarium sp. 3300 (NRRL 25102) inhibit the growth and biofilm formation of S. aureus ATCC 6538. The fungus was cultured on a dextrose potato broth in the absence (A) and in the presence of 2% (w\/v) pups of Ceratitis capitata (B), using as a control the medium with insect (C). After 15 days incubation, the supernatants was separated of the mycelium by filtration, the supernatant was extracted with ethyl acetate and the FM produce in the different media were analyzed by TLC. Eighteen eluates were obtained in the B condition; eight of them were produced by the insect induction. The extract obtained from A and B conditions, as well as, four elautes produced by induction have bactericidal activity against S. aureus ATCC 6538. The production of substances in presence of insect-derived material suggested that new antimicrobial compounds are produce when the fungus infects the insect. This substances of eukaryotic origin, and normally present into the environmental, should be study as potential antibiotics","palabras_clave":"Fusarium|Staphylococcus aureus|BACTERICIDAL|FUNGAL METABOLITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58429,"idioma_id":4,"titulo":"LPKI: A novel serine proteinase inhibitor as a potential biochemical marker involved in horizontal resistance to","resumen":"Potato leaves infected with P.infestans produced a serine proteinase  inhibitor (PLPKI) with specificity for microbial proteinases. Sequencing of the first twenty residues at the NH2-terminus of the mature PLPKI  polypeptide demonstrated that PLPKI is a novel member of the potato  proteinase inhibitor I family. PLPKI inhibited the activity of extracellular proteinases produced by two pathogens of potato, P. infestans and R. solani, but was inactive against proteinases secreted into the culture media by the binucleate Rhizoctonia N2, a non-pathogenic fungus for potato. Western blot analysis showed a positive correlation between the levels of PLPKI and the degree of horizontal resistance, showing its highest accumulation in clone OKA 5632.11, which has been described as highly resistant. This correlation, together with the ability of PLPKI to completely abolish the secreted serin proteinase activity of P. infestans, suggests that PLPKI may have an active role in protecting potato plants from this pathogenic oomycete and that it could be used as a suitable biochemical marker to help breeders in the selection of cultivars with high degree of horizontal  resistance.","palabras_clave":"PROTEINASE INHIBITOR|PAPA|RESISTANCE|PHYTOPHTHORA INFESTANS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58561,"idioma_id":5,"titulo":"Apropiación  y uso del decodificador digital en la ciudad de Paraná","resumen":"Este artículo pretende dar cuenta del trabajo de investigación realizado en el marco de la práctica curricular de la Licenciatura en Comunicación Social de la Universidad Nacional de Entre Ríos, sobre la Televisión Digital Terrestre, en especial, el plan nacional Mi TV Digital, como una de las políticas comunicacionales más importante de los últimos diez años. Se pretendió indagar sobre el uso, la apropiación y el consumo del modelo televisivo impulsado por el Estado Nacional en la ciudad de Paraná, provincia de Entre Ríos, durante el año 2012. Para esto se realizó un estudio de corte cuantitativo a base de encuestas con cuestionarios semi-cerrados sobre los alcances del plan nacional de entrega de equipamiento de recepción gratuita, ?Mi Tv Digital?, para tratar de dilucidar tendencias o líneas de análisis en vista a futuros estudios, reflexiones, debates sobre la temática.","palabras_clave":"TELEVISIÓN DIGITAL|AUDIENCIA|CONSUMO CULTURAL|ESTUDIOS DE AUDIENCIA|MEDIOS DE MASAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58617,"idioma_id":5,"titulo":"Microfluídica y biotecnología. Descripción de los objetivos y de las capacidades del laboratorio de Micro y Nanofluídica y Plasma de la Facultad de Ingeniería de la UdeMM","resumen":"En este trabajo se presenta una introducción a la microfluídica y a la biotecnología. Luego se presentan los objetivos y capacidades del laboratorio de Micro y nanofluídica y Plasma de la UdeMM.","palabras_clave":"Microfluídica|Biotecnología"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58641,"idioma_id":5,"titulo":"Reseña de RODRIGUEZ, Fermín, Un desierto para la nación: la escritura del vacío, Buenos Aires, Eterna Cadencia Editora, 2010, 409 págs., ISBN 978-987-1673-15-5.","resumen":"En un aleatorio ida y vuelta entre el pasado y el presente 'Un desierto para la nación', de Fermín A. Rodríguez, transita rizomáticamente la literatura del desierto argentino y propone así una lectura original sobre ese 'espacio informe' que, hacia el siglo XIX, se percibió como un espacio vacío, una geografía que tanto la literatura como el Estado Nación se han esforzado en poblar.","palabras_clave":"desierto|nación|literatura|capitalismo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58817,"idioma_id":4,"titulo":"Inhibition behavior on alkaline phosphatase activity, antibacterial and antioxidant activities of ternary methimazole-phenanthroline-copper(II) complex.","resumen":"Methimazole (MeimzH), a sulfur containing compound, is an antithyroid drug commonly used to treatGraves? disease acting as an inhibitor of the enzyme thyroid peroxidase. A new ternary [Cu(MeimzH)2(phen)(H2O)2]Cl2 (Cu-Met-phen) complex of this drug containing also phenanthroline as a second ligand(phen) was synthesized and characterized by elemental analysis, dissolution behavior, thermogravimetricanalysis, UV?Vis, diffuse reflectance, FTIR and EPR spectroscopies. As it is previously reported, the binary[Cu(MeimzH)2(H2O)2](NO3)2H2O (Cu-Met) complex can act as an inhibitor of alkaline phosphatase(ALP). In this work, the inhibitory effect of methimazole, phenanthroline, [Cu(phen)2Cl]Cl (Cu-phen)and the new ternary complex has also been investigated and compared with Cu-Met together within vitro tests of susceptibility against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus,Staphylococcus epidermidis and Enterococcus faecalis bacteria. To our knowledge, the antibacterial activityof methimazole is determined for the first time. For the ernary complex, the results show that the coordinationof the drug increased its antibacterial effect especially against E. faecalis demonstrating a strongeffect against S. aureus and S. epidermidis. The assays also indicate that in same strains the new complexshow better activity than their ligands. The ability for the inhibition of alkaline phosphatase (ALP) waslower than that of the binary complex (Cu-Met) probably due to the absence of labile ligands aroundthe metal center. Moreover, the antioxidant activity of the complexes decreases as follows: Cu-Met > Cu-Phen > Cu-Met-Phen. 2013 Elsevier B.V. All rights reserved","palabras_clave":"METHIMAZOLE|COPPER COMPLEXES|ANTIMICROBIAN ACTIVITY|ALP INHIBITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58829,"idioma_id":4,"titulo":"Split clique graph complexity","resumen":"A {it complete set} of a graph $G$ is a subset of vertices  inducing a complete subgraph.  A {it clique} is a maximal complete set.  Denote by ${ mathcal{C}}(G)$ the {it clique family} of $G$.  The {it  clique graph} of $G$, denoted by $K(G)$,  is the intersection graph of $ mathcal{{C}}(G)$.  Say that $G$ is {it a clique graph}  if there exists a graph $H$ such that $G=K(H)$.  The clique graph recognition problem,  a long-standing open question posed in 1971,  asks whether a given graph is a clique graph  and it was recently proved to be NP-complete even for a graph $G$ with  maximum  degree 14 and maximum clique size 12.  Hence, if P$  e$NP, the  study of graph classes where the problem can be proved to be  polynomial, or of more restricted graph classes where the problem  remains NP-complete is justified.  We present a proof that given a split graph $G=(V,E)$ with partition  $(K,S)$  for $V$, where $K$ is a complete set and $S$ is a stable set,  deciding whether there is a graph $H$ such that $G$ is the clique graph of  $H$ is NP-complete.  As a byproduct,  we prove that determining whether a given set family admits a spanning family  satisfing the Helly Property is NP-Complete.  Our result is optimum in the sense that each vertex of the independent set  of our split instance has degree at most 3,  whereas when each vertex of the independent set has degree at most 2  the problem is polynomial, since it is reduced to the problem of checking  whether the clique family of the graph satisfies the Helly property.  Additionally, we show three split graph subclasses  for which the problem is polynomially solvable:  the subclass where each vertex of $S$ has a private neighbor,  the subclass where $|S|leq 3$,  and the subclass where $|K|leq 4$.","palabras_clave":"Intersection graphs|clique graph|computational complexity theory"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":58983,"idioma_id":5,"titulo":"Estimación de Frecuencia y Distorsión Armónica Total Basada en Filtros Adaptativos","resumen":"In this paper, a low computacional cost total  harmonic distortion and frecuency estimation method is  proposed. This method is based on adaptive digital filters  allowing tracking the fundamental frequency variations. Real  time experimental results obtained using a fixed point digital  signal processor development kit are presented. They show an  acceptable performance and have a robust behavior to signal  distortions such as frequency fluctuations, high harmonic  content, subharmonic and interharmonics.","palabras_clave":"Adaptive filters|frequency estimation|interharmonics|power quality|subharmonics|total harmonic distortion."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59004,"idioma_id":5,"titulo":"Los estudios sobre estructura social en la Argentina  y la reconfiguración de la clase trabajadora","resumen":"Durante décadas el estudio del mundo del trabajo en nuestro país ha sido moneda poco corriente en la agenda académica. No obstante, con la recomposición de los índices de empleo en Argentina (y un consecuente aumento de la conflictividad laboral) es posible identificar un conjunto de investigaciones que buscan saldar esta carencia.  En este artículo buscamos aportar algunos elementos acerca de los cambios producidos en la clase trabajadora en el período de consolidación de una nueva etapa de acumulación del capital en nuestro país.","palabras_clave":"ESTRUCTURA SOCIAL|CLASE TRABAJADORA|MERCADO DE TRABAJO|NEOLIBERALISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59059,"idioma_id":5,"titulo":"ESTRUCTURA Y FUNCIONAMIENTO DE UNA PROMOCION DE ESPECIES INVERNALES BAJO CLAUSURA","resumen":"EL OBJETIVO FUE CARACTERIZAR LA COMPOSICIÓN FLORISTICA Y ESTIMAR LA PRODUCTIVIDAD PRIMARIA NETA AÉREA (PPNA) ESTACIONAL Y ANUAL DE UNA PROMOCIÓN DE ESPECIES INVERNALES.","palabras_clave":"COMPOSICION FLORISTICA|COBERTURA|GRAMINEAS|PPNA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59063,"idioma_id":5,"titulo":"EFECTOS DE PASTOREO SOBRE UNA PROMOCION DE FORRAJERAS INVERNALES","resumen":"EL OBJETIVO DEL TRABAJO FUE DETERMINAR LOS EFECTOS DE LA CARGA ANIMAL SOBRE LA ESTRUCTURA Y EL FUNCIONAMIENTO DE UNA PROMOCIÓN QUÍMICA DE FORRAJERAS INVERNALES.","palabras_clave":"COMPOSICION FLORISTICA|COBERTURA|DIVERSIDAD|CRECIMIENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59203,"idioma_id":5,"titulo":"Discontinuidad y diferencia como variaciones de la metáfora de la distancia histórica. El caso de la genealogía pesimista","resumen":"En este trabajo nos proponemos revisitar la cuestión de la metáfora de la distancia histórica a partir de los conceptos de 'discontinuidad' y 'diferencia'. Sostendremos que la distancia histórica es tanto dada como construida, y que en este sentido se sirve de distintos tipos de mediadores que permiten establecer diversas relaciones entre el presente y el pasado.    Para desarrollar el análisis tomaremos como ejemplo lo que hemos denominado el uso 'pesimista' que Foucault hace de la reconstrucción genealógica del pasado y lo contrastaremos con el uso que Richard Rorty hace de la misma. Nuestro objetivo es poner de manifiesto que los distintos usos de la genealogía, entendida como una reconstrucción anacrónica del pasado, suponen el empleo inevitable de creencias desde un presente.","palabras_clave":"Filosofía de la Historia|Distancia histórica|Genealogía|Michel Foucault"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59216,"idioma_id":4,"titulo":"CHARACTERIZATION OF STRAWBERRY JUICE CULTIVATED IN NORTHWEST ARGENTINEA. EFFECT ON FOOD PATHOGENS","resumen":"The aim of this work is to characterize sugars and phenolic compounds profiles of strawberry juice cultivated in Tucuman and evaluate the antimicrobial effect of juice against pathogenic bacteria isolated from food. Strawberries juice (J) was extracted; a fraction was clarified (CJ). Total phenols (TP) were determined using the Folin-Ciocalteu reagent. FT and the carbohydrate profile were determined by HPLC. Salmonella enteritidis, Salmonella typhimurium and Shigella flexnerii, were growth in BHI media supplemented with J or CJ, incubated 24h 37°C and determined optical density and cfu\/ml. The J contains TP (890 g GAE \/ ml), ellagic acid, ferulic acid, gallic, coumaric and in low concentrations, cinnamic acid and kaempferol. In J, fructose and glucose are majority sugars, while sucrose concentration was three folds lower. The growth of S. enteritidis and S. typhimurium was inhibited by J and JC, but the effect of J was 50% greater than that observed with CJ, indicating that the principal antibacterial effect is due to TP. Sh. flexnerii growth was not affected by J or CJ. These results are an important contribution to biotechnology considering the future application of the phenolic compounds in strawberry juices as natural antimicrobials in food","palabras_clave":"STRAWBERRY |JUICE|PATHOGENS|FOODS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59218,"idioma_id":4,"titulo":"GLUCOSIDASE ENZYMES AND AROMA COMPOUNDS PRODUCTION BY Oenococcus oeni IN A WINE LIKE MEDIUM","resumen":"La fermentación maloláctica llevada a cabo por las bacterias lácticas, disminuye la acidez de vinos, le confiere estabilidad biológica y puede incrementar su riqueza aromática. Diacetilo, acetoína y 2,3 butanodiol (compuestos de 4C), los cuales derivan del ácido cítrico vía ácido pirúvico y, sustancias liberadas a partir de la hidrólisis de precursores glicoconjugados no aromáticos por acción de enzimas glucosidasas son compuestos aromáticos de interés. Nos propusimos investigar las actividades &#946;-glucosidasa y &#945;- arabinofuranosidasa y producción de los compuestos de 4C durante el crecimiento de O. oeni MS9, MS20 y MS46, de vino argentino, en un medio de composición similar a vino (MV) a 30ºC y, la influencia del ácido pìrúvico (1 g\/l) o el glicósido sintético Eriocitrin (1mg\/ml) sobre las mismas. Las células fueron pre-adaptadas e inoculadas en los medios de pruebas (orden 106 ufc\/ml) de pH 4.8. Las actividades enzimáticas se ensayaron en células enteras, utilizando p-nitrofenil-glucósidos como sustratos. Una unidad enzimática (U) se definió como µmoles de p-nitrofenol liberado por minuto. Los compuestos de 4C se determinaron como un valor combinado por método espectrofotométrico. En medio MV, las cepas incrementaron sus concentraciones celulares finales alrededor de 1 ciclo log, las actividades enzimáticas detectadas desde el inicio del crecimiento, alcanzaron valores máximos a final de fase exponencial, especialmente en O. oeni MS9 y MS46 (entre 7 y 10 U) y los compuestos de 4C no superaron 3 mg\/l. La adición del glicósido sintético o piruvato no modificaron significativamente sus crecimientos. En presencia del primero las actividades &#946;- glucosidasa y &#945;-arabinofuranosidasa incrementaron entre 60-80 y 80-90%, respectivamente, especialmente en la cepa MS46, indicando sus potencialidades aromáticas. Solamente en presencia de piruvato los compuestos de aroma incrementaron por encima del valor considerado aceptable en vinificación para diacetilo (4-5 mg\/l). Así, las cepas en estudio en el medio natural no producirían cantidades suficientes del intermediario metabólico para formar los compuestos de 4C en concentraciones que alteren la calidad del vino.","palabras_clave":"GLUCOSIDASE ENZYMES|AROMA COMPOUNDS |Oenococcus oeni|WINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59263,"idioma_id":5,"titulo":"Las marcas de la inundación","resumen":"Cuando el agua negra marcó las casas como una cicatríz, todos supimos que  nada en La Plata volvería a ser igual. La ciudad que se fundó bajo los ideales  iluministas (con ideas de ?iluminados?) quedó paradójicamente a oscuras, y  a veces cuando está oscuro todo empieza a verse más claro. En la horas y los  días que siguieron se constató un saber ancestral, que durante años durmió, o  desapareció, o simplemente esperó. Y hoy se actualiza y despliega en una nueva  marca: la de la militancia","palabras_clave":"CIUDAD|INUNDACIONES|UNIVERSIDAD|MILITANCIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59361,"idioma_id":4,"titulo":"CENTRAL NERVOUS SYSTEM INFECTIONS DUE TO  Nocardia asteroides (ABSTRACT)","resumen":"Nocardia asteroides is a gram-positive, partially acid-fast, soil-bornaerobic actinomycete that causes both localized and disseminatedinfection. Nocardiosis is typically regarded as an opportunistic infection,uncommon cause of intracranial abscesses, which comprise 1%to 2% of all the cerebral abscesses.Nocardia asteroides infection is acquired from the environmentthrough inhalation into the respiratory tract, which leads to pulmonarydisease. Subsequent hematogenous dissemination occurs from thelungs, which leads to cerebral abscess formation as well as cutaneousskin lesions.We report a case from a 24 years old woman, ( she did not use HIVtherapy), who developed a cerebral mass lesion 10 month before andunder suspected diagnosis of toxoplasmosis started treatment withnot clinical or radiological improviment; craniotomy with abscessevacuations was performed. Culture of brain biopsy and abscessspecimens development a Gram- positive fi lamentous rods and drywithe colonies identifi ed as Nocardia asteroides by biochemical characterizationand susceptibility antibiotic patterns. The mycobacterialculture informs the same results.Species identifi cations by the laboratory can have an inmediateimpact in the therapy","palabras_clave":"CENTRAL NERVOUS SYSTEM|NOCARDIA ASTEROIDES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59495,"idioma_id":5,"titulo":"Los medios y la disputa por la construcción de sentido","resumen":"La idea de pensar los medios en relación a la construcción social de sentido parte de un supuesto sobre el que tal vez sea necesario detenerse un momento: el de que los sentidos son construidos. Detenerse en la idea de que los sentidos se construyen, es decir, detenerse en la idea de que los sentidos sobre la vida social son sentidos históricos. No son ?verdaderos?, no están dados de una vez y para siempre, por lo tanto pueden ser de otra forma, pueden ser cuestionados.Lo que muchas veces llamamos cultura puede ser pensado como sentido común: como un común sentido sobre las diversas dimensiones de la vida (sobre las definiciones y clasificaciones acerca de lo bello, lo triste, lo ominoso? sobre qué es lo correcto y lo incorrecto? sobre lo permitido y lo prohibido? sobre quiénes son los sujetos valiosos y quiénes en cambio son los sujetos que no valen, o son peligrosos, y deberían engrosar el espacio donde se tiran los desperdicios?). Escuchamos muy seguido la expresión ?es de sentido común? cuando alguien quiere referirse a una verdad evidente que necesita ser reconfirmada para aquel que no la ve. Decir que es de sentido común equivale entonces a decir que algo, que alguna información, es verdadera.Pero el sentido común no es verdadero en sí mismo, sino que está sostenido en relaciones históricas de poder. Siguiendo la tradición gramsciana, podríamos hablar de hegemonía: esa dirección político ideológica de una clase, sector de clase, bloque dominante que hace que sus ideas sean consideradas las únicas ideas posibles y deseables para toda la sociedad.Los sentidos sobre la vida, se viven como si siempre ?hubiera sido así?, como si siempre ?fueran a ser así?. Por eso, marcar que en esta afirmación se esconde la historia implica pensar que no son nada naturales (pocas cosas son naturales, digámoslo de paso, en el mundo de los humanos).","palabras_clave":"MEDIOS|CONSTRUCCION|SENTIDO|HEGEMONÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59519,"idioma_id":5,"titulo":"El empleo de la arquitectura en los medios de prensa. Precisiones sobre la propaganda política del primer peronismo en la provincia de Mendoza (Argentina).","resumen":"El presente trabajo aborda la relación de la fotografía y la arquitectura. Más precisamente, se centra en la circulación de imágenes de la disciplina que se sucedió en la prensa de la provincia de Mendoza, durante la primera mitad del siglo XX. A partir de la lectura y seguimiento de las fotografías divulgadas en estos años en diarios y revistas locales, se evidencia un proceso de afianzamiento del empleo de las reproducciones de obras de arquitectura como una herramienta para exponer distintas ideas. Durante el período estudiado podemos distinguir un desplazamiento del interés en la difusión de estas imágenes. Primeramente apreciamos la reproducción de edificaciones por parte de privados como muestra de su poder adquisitivo, luego a partir de que estos procedimientos técnicos se hacen más usuales, observamos cómo la fotografía de obras de arquitectura fue empleada como propaganda política por parte del gobierno. Esta utilización encontró su máxima expresión durante el peronismo, período en que nos centraremos más extensamente debido a su riqueza en lo que se refiere al empleo de la arquitectura como propaganda política.","palabras_clave":"ARQUITECTURA|PRENSA|PERONISMO|PROPAGANDA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59539,"idioma_id":5,"titulo":"'Demonios, profetas y mártires. Restos bíblicos en la ensayística hispanoamericana moderna'","resumen":"El  ensayo nació en Hispanoamérica como un género crítico del colonialismo. El  espíritu rebelde que lo caracteriza le ha impedido rendirse ante 'verdades  reveladas'. Esta circunstancia ?el espíritu crítico? se ha mantenido a lo  largo del tiempo y es la marca que aún hoy lo distingue. En consecuencia, esa  característica lo ha alejado de todo dogmatismo (nos referimos desde luego a  los ensayos canónicos o que merezcan la categoría de logrados en su forma). Los  restos bíblicos en el género no abundan por lo dicho y además como consecuencia  de la 'sacralización del mundo' que ha ocupado la conciencia americana  de nuestros ensayistas. Ello no ha impedido, de todos modos, que el  'universo mitológico' del Gran Código (la Biblia) del que habla  Northrop Frye no penetre los textos de diversos modos. De manera alegórica en  algún caso, inconscientemente en otros, pero como estereotipos en la mayoría.  Tampoco podemos dejar de aludir a que el Gran Código también afecta la  estructura del género ensayístico en cuanto a la praxis profética o redentora  en la que los mejores exponentes del género han fusionado radicalmente la idea  con la vida.","palabras_clave":"ensayo hispanoamericano| mitos bíblicos|teorías del arte y el intelectual"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59542,"idioma_id":5,"titulo":"El ensayo entre la desmesura y el desmenuzamiento. Lectura de Inquisiciones\/Otras Inquisiciones.","resumen":"La reedición de dos libros de ensayos de Borges ?Inquisiciones y Otras Inquisiciones- constituye una novedad de mucho interés en virtud de que la aparición de estos textos  estuvo separada por casi 30 años uno de otro. Mediante una lectura de conjunto de los ensayos que contiene el reciente volumen se obtiene un nuevo sentido estético en el orden de la forma, el tratamiento de algunos temas, la figura del yo. Asimismo se abren nuevas oportunidades de exploración del ensayo de Borges no como un género subordinado a la interpretación de los cuentos o la poesía.","palabras_clave":"Borges|ensayo| escrituras del yo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59589,"idioma_id":4,"titulo":"TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes","resumen":"Mutations in the gene coding for the transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI) are found in 8% to 10% of subjects with common variable immunodeficiency (CVID). Although heterozygous mutations may coincide with immunodeficiency in a few families, most mutation-bearing relatives are not hypogammaglobulinemic. Thus, the role of TACI mutations in producing the immune defect remains unclear. Objective: This study examined the expression and function of TACI mutations in healthy heterozygous relatives. Methods: We examined the surface and intracellular expression of TACI protein in EBV-transformed B cells of patients and relatives with mutations in 7 families, binding of a proliferationinducing ligand, and secretion of IgG and IgA by ligandactivated B cells.We tested whether Toll-like receptor 9 agonists increased TACI expression and whether an agonistic anti-TACI antibody could induce activation-induced cytidine deaminase mRNA in those with mutations. Results: Intracellular and extracellular TACI expression was defective for B cells of all subjects with mutations, including subjects with CVID and relatives. Although Toll-like receptor 9 triggering normally up-regulates B-cell TACI expression, this was defective for all subjects with mutations. Triggering TACI by an agonistic antibody showed loss of activation-induced cytidine deaminase mRNA induction in all mutation-bearing B cells. However, ligand-induced IgG and IgA production was normal for healthy relatives but not for subjects with CVID. Conclusion: Thus, B cells of relatives of subjects with CVID who have mutations in TACI but normal immune globulin levels still have detectable in vitro B-cell defects.","palabras_clave":"CVID|TACI|Hypogammaglobulinemia|Activation induced cytidine deaminase"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59620,"idioma_id":4,"titulo":"Stand recovery and self-organization following large-scale mountain pine beetle induced canopy mortality in northern forests","resumen":"A mountain pine beetle (MPB) epidemic is currently ravaging large areas of interior British Columbia (BC) with significant implications for ecosystem services including future timber supply and community economic stability. Information is needed on future stand dynamics in areas of impacted forests that are unlikely to be salvaged logged. Of greatest concern are stands dominated by lodgepole pine (&gt;50% timber volume). Predicting how surviving trees in these areas respond and grow and the timing and specie composition of natural regeneration ingress is of critical importance for multiple forest values. We undertook a retrospective study in the Flathead Valley of southeastern British Columbia where an intense MPB epidemic peaked in 1979?1980. Our objective was to gain insight into stand recovery and stand selforganization as influenced by species-specific growth responses of different sized secondary structure trees (individual seedling, sapling, sub-canopy and canopy trees surviving the epidemic) and post-beetle regeneration dynamics. MPB mortality rates, the percent of basal area killed by beetles, varied from 42%to 100% with most stands between 60% and 80%. In general, all surviving secondary structure released but the extent of growth release exhibited species variability. Release of surviving canopy lodgepole pine trees was often dramatic and greatest in stands with high total stand MPB mortality rates. Ingress of natural regeneration was slow in the first few years after MPB attack but there was a strong pulse of recruitment 10?20 years post disturbance which then slowed considerably. Nearly 30 years after the MPB attack, the stocking and composition of the understories have changed dramatically. Overall, the occurrence of the MPB epidemic resulted in more structurally and compositionally diverse stands leading to multiple successional pathways different from those of even-age pine dominated stands. The recovery and self-organization of unsalvaged natural stands in the Flathead Valley was a complicated process. It has provided insights for future forest management in areas impacted by the current massive MPB epidemic ongoing for the past decade in western North America.","palabras_clave":"mountain pine beetle|regeneration|mortality"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59693,"idioma_id":4,"titulo":"Inteligencia Artificial. Guest Editorial: XIII Workshop on Agents and Intelligent Systems - Selected papers","resumen":"This special issue contains fives extended papers thatwere selected as the best articles from the XIII Workshop on Agents an Intelligent Systemas. This workshop was held al Universidad Nacional del Sur, Bahía Blanca Argentina between 9th and 11th November 2012. The Workshop on Agents and Intelligent Systemsis held yearly as a joint event during the Argentine Congress on Computer Science, an Argentine Conference which has been held yearly from 1995.","palabras_clave":"AGENTES INTELIGENTES|SISTEMAS INTELIGENTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59786,"idioma_id":5,"titulo":"La Historia Social de la Salud y la Enfermedad: conformación, logros y desafíos","resumen":"En este trabajo mostraremos el camino de las transformaciones teórico-metodológicas  que han atravesado el campo de estudio denominado ?Historia social de la salud y la enfermedad?. Procuraremos así sondear un campo de investigación en expansión, donde se potencia la capacidad explicativa de la historia, pero indagando la articulación de diversos abordajes ligados al proceso de salud-enfermedad-atención. El artículo desarrolla tres bloques. En primer lugar, ponemos en perspectiva las matrices desarrolladas en Europa y Estados Unidos. En segundo lugar, abordamos la historia de la salud y la enfermedad en América Latina, las influencias internacionales y los actuales desarrollos. Por último, indagamos sobre la agenda en Argentina así como la propulsada desde los núcleos de estudios locales.","palabras_clave":"Historia social de la salud y la enfermedad|renovacion teorico metodologica|America Latina|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59880,"idioma_id":4,"titulo":"Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process","resumen":"Discoidal high-density lipoproteins (D-HDL) are critical intermediates in reverse cholesterol transport. Most ofthe present knowledge of D-HDL is based on studies with reconstituted lipoprotein complexes of apolipoproteinA-I (apoA-I) obtained by cholate dialysis (CD). D-HDL can also be generated by the direct microsolubilization(DM) of phospholipid vesicles at the gel\/fluid phase transition temperature, a process mechanistically similarto the ?in vivo? apoAI lipidation via ABCA1. We compared the apoA-I configuration in D-HDL reconstitutedwith dimyristoylphosphatidylcholine by both procedures using fluorescence resonance energy transfermeasurementswith apoA-I tryptophan mutants and fluorescently labeled cysteine mutants. Results indicate that apoA-Iconfiguration in D-HDL depends on the reconstitution process and are consistentwith a ?double belt? molecular arrangementwith different helix registry. As reported by others, a configuration with juxtaposition of helices 5 of each apoAImonomer (5\/5 registry) predominates in D-HDL obtained by CD. However, a configurationwith helix 5 of one monomer juxtaposedwith helix 2 of the other (5\/2 registry) would predominate in D-HDL generated by DM.Moreover, we also show that the kinetics of cholesterol efflux from macrophage cultures depends on the reconstitution process, suggesting that apoAI configuration is important for this HDL function","palabras_clave":"APOLIPOPROTEINS|FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET)|LIPOPROTEIN STRUCTURE|SITE DIRECTED MUTAGENESIS|SINGLE TRYPTOPHAN MUTANTS|CYSTEINE MUTANTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59882,"idioma_id":4,"titulo":"Lactic acid bacteria suitable for silage inoculants in the inhibition of Aspergillus parasiticus and Fusarium gramineraum growth and mycotoxin production.","resumen":"Aims: to select lactic acid bacteria with potential silage inoculant properties. The bio-control activity against mycotoxicogenic fungi and the presence of antibiotics resistance gene were also evaluated. Methods and Results: Lactobacillus rhamnosus RC007 and Lactobacillus plantarum RC009 were selected on the basis of growth rate and efficacy in reducing the pH of maize extract medium, therefore they were evaluated for their bio-control ability against Fusarium graminearum and Aspergillus parasiticus. Studies on lag phase, growth rate and aflatoxin B1 (AFB1) and zearalenone (ZEA) production were carried out in vitro under different regimes of aw (0.95 and 0.99); pH (4 and 6); temperature (25 and 37°C) and oxygen availability (normal and reduced). Lactobacillus rhamnosus RC007 was able to completely inhibit the F. graminearum growth at all assayed conditions, while L. plantarum RC009 only did it at pH 4. Both Lactobacillus strains were able to significantly reduce the A. parasiticus growth rate mainly at 0.99 aW. A decrease of ZEA production was observed as result of Lactobacillus strains ?F. graminearum interaction; however the A. parasiticus- L. plantarum interaction resulted in an increased AFB1 production. Lactobacillus rhamnosus RC007 proved to have no genes for resistance to the tested antibiotics. Conclusions: The ability of L. rhamnosus RC007 to rapidly drop the pH and to inhibit fungal growth and mycotoxin production and the absence of antibiotic resistance genes, shows the potential of its application as inoculant and bio-control agent in animal feed. Significance and Impact of the Study: This study demonstrated the importance of selecting bacteria for silage inoculants not only for the improvement of silage fermentation but also for their effects on mycotoxicogenic fungi and the resulting mycotoxin production due to the risk that they may involve.","palabras_clave":"Lactic acid bacteria|silage inoculants |Aspergillus parasiticus |Fusarium gramineraum"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":59981,"idioma_id":4,"titulo":"Methodology for predicting the energy consumption of SPMD application on virtualized environments","resumen":"Over the last decade, the computing clusters have been updated in order to satisfy the increasing demand of greater computational power for running applications. However, this increasing is transformed in more system en- ergy consumption, which results in financial, environmental and in some cases with social consequences. Hence, the ideal is to achieve an scenario that allows the system admin- istrator to find a trade-off between time and energy-efficiency for parallel algorithms on virtualized environments. The main objective of this work is based on developing an analytical model to predict the energy consumption and energy delay product (EDP) for SPMD applications on virtual environments. The SPMD applications selected are designed through a message passing interface (MPI) library with high communication volumes, which can generate im- balance issues that affect seriously the execution time and also the energy-efficiency. Our method is composed by four phases (characterization, tile distribution model, mapping and scheduling). This method has been validated using scientific applications and we observe that the minimum Energy and EDP values are located close to the values calculated with our analytical model with an error rate between 4% and 9%.","palabras_clave":"performance|energy prediction|virtualization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60112,"idioma_id":5,"titulo":"Periodismo y nuevas tecnologías: los ejes del pensamiento hegemónico y una posible contra agenda","resumen":"La relación entre periodismo y nuevas tecnologías ha sido pensada históricamente  escindida de la dimensión del poder, separando la técnica de su espesor social desde  una perspectiva instrumental de la misma funcional a una concepción administrativa  liberal del periodismo. En la actualidad la pregunta por ese vínculo implica indagar si  el periodismo asume la explicación neoliberal del mundo que lo condena a ser mera  herramienta de un orden reproductor de la palabra de unos pocos; o si se posiciona en los  procesos de construcción de emancipación de su pueblos ayudando a parir las voces, los  olores, los sonidos, las imágenes y la lengua de aquellos que luchan por un horizonte de  justicia.","palabras_clave":"PERIODISMO|TECNOLOGÍAS|PODER|COMUNICACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60122,"idioma_id":5,"titulo":"Estudio genotóxico del  extracto acuoso de Aristolochia macroura.","resumen":"Dominguezia - Vol. 29(Suplemento) ? 2013  A5-28. ESTUDIO GENOTÓXICO DEL EXTRACTO  ACUOSO DE ARISTOLOCHIA  MACROURA. G. S. Jaime¹,²*, G. E. Barboza³, M.  A. Vattuone4. 1 Instituto de Estudios Vegetales.  Facultad de Bioquímica, Química y Farmacia.  Universidad Nacional de Tucumán. Ayacucho 471  (4000), Tucumán, Argentina. 2 Universidad Nacional  de Chilecito.La Rioja. 3 IMBIV- Universidad  Nacional de Córdoba. 4 CONICET.  *gsjaime@fbqf.unt.edu.ar  Aristolochia macroura Gómes, popularmente conocida  como mil hombres, patito, buche de pavo,  entre otros, es una planta que habita la región este y  noreste de la Argentina. Se comercializan en mercados,  herboristerías y farmacias fragmentos de tallos  que se utilizan en infusiones por sus propiedades  medicinales tradicionales. Se han informado sus  usos etnomédicos diversos, como antirreumático,  emenagogo, abortivo, anti-inflamatorio y antiséptico, entre otros. Especies del género Aristolochia se  han informado como drogas tóxicas y se ha demostrado  que el extracto metanólico de hojas tiene efecto  citotóxico sobre líneas celulares hepáticas de carcinoma  humano, HepG2. En este trabajo se analiza  los efectos citotóxico y genotóxico del extracto acuoso  de tallos de A. macroura por medio del Test de  Allium cepa, para validar su uso terapéutico tradicional.  Se evaluó el extracto vegetal en el ensayo de  células de A. cepa determinando variables como el  crecimiento radicular, el índice mitótico y las aberraciones  cromosómicas. Todas estas variables fueron  afectadas al exponer los meristemas radiculares  de cebolla a distintas concentraciones de extracto  (10, 100, 1 000 y 10 000 ppm) durante 72 horas. La  citotoxicidad se manifestó en la longitud radicular,  que disminuyó más del 50 % en todos los tratamientos;  se observarone cambios en la morfología  radicular con ápices espatulados, gruesos y  tumoraciones a las mayores concentraciones. En el  nivel microscópico se detectaron megacélulas, mayor  vacuolización, y genotoxicidad con la aparición  de aberraciones cromosómicas a partir de concentraciones  de 100 ppm, tales como cromosomas fragmentados,  puentes y c- mitosis. El índice mitótico  (IM) disminuyó de 57 % (100 ppm) a 34 % (10000  ppm), y se manifestó significativamente en la inhibición  del ciclo celular. El extracto de A. macroura  muestra un evidente efecto inhibitorio, en el modelo  experimental, que tiende a detener la proliferación  celular.  Palabras clave: citotoxicidad - genotoxicidad -  Aristolochia macroura.","palabras_clave":"CITOTOXICIDAD|GENOTOXICIDAD|ARISTOLOCHIA MACROURA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60124,"idioma_id":5,"titulo":"ESTUDIO GENOTÓXICO DE CAESALPINIA PARAGUARIENSIS","resumen":"A5-29. ESTUDIO GENOTÓXICO DE  CAESALPINIA PARAGUARIENSIS. G. S. Jaime1,2*,  M. A. Sgariglia1,3, J. R. Soberón1,3, D. A.  Sampietro1,3, M. A. Vattuone3. 1 Instituto de Estudios  Vegetales, Facultad de Bioquímica, Química  y Farmacia, Universidad Nacional de  Tucumán. Ayacucho 471 (4000), Tucumán, Argentina.  2 Universidad Nacional de Chilecito. La  Rioja. 3 CONICET. *gsjaime@fbqf.unt.edu.ar  Caesalpinia paraguariensis (D. Parodi) Burkart  (Caesalpiniae) es un árbol autóctono de la Argentina,  cuya corteza es empleada medicinalmente por  sus propiedades vulnerarias. Estudios previos indican  que el extracto etanólico de esta planta es  citotóxico sobre larvas de Artemia salina y fuertemente  genotóxico por Bacillus subtilis Rec-assay.  El propósito de este trabajo fue analizar el efecto  genotóxico del extracto etanólico (EECCP) por  medio del Test de Allium cepa (cebolla), buscando  observar cómo afecta la mitosis y si genera alteraciones  cromosómicas. Se ensayaron distintas concentraciones  de EECCP (10, 100 y 1 000 ppm) y se  analizó el efecto sobre el desarrollo de las raíces de  cebolla midiendo su longitud. Las alteraciones causadas  en la división celular de meristemas apicales  radiculares se analizaron por microscopía óptica, y  se obtuvieron preparados a partir de ápices  radiculares teñidos con orceína acética, y posterior  aplicación de la técnica squash. Se observaron un  total de 3 500 células\/campo para cada una de las  concentraciones ensayadas. Se evaluaron parámetros  macroscópicos y microscópicos, como la presencia  de cofias desgarradas, necrosis radicular, aberraciones  cromosómicas, como c- mitosis, cromosomas  fragmentados, presencia de puentes . Los resultados  obtenidos mostraron que a 100 ppm se produce  50 % de inhibición del crecimiento radicular respecto  del control, establecida como la dosis  citotóxica. A la misma concentración se observó  efecto antimitótico, con un IM de 56 %. Además, el  IM de cada concentración está relacionado significativamente  con la inhibición en longitud de las  raíces, a 10 ppm: IM 60,54 %, 100 ppm: IM 56 %, 1  000 ppm: IM 47,31 %. A 1 000 ppm se observaron  aberraciones y anomalías cromosómicas, como  puentes y cromosomas fragmentados. El efecto  antimitótico observado permite considerar a EECCP  como posible agente antitumoral.  Palabras clave: genotoxicidad - antimitótico -  Caesalpinia paraguariensis.","palabras_clave":"GENOTOXICIDAD|ANTIMITOTICO|CAESALPINIA PARAGUARIENSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60316,"idioma_id":5,"titulo":"Un proceso de enseñanza-aprendizaje como itinerario de conocimiento","resumen":"En este artículo se toman aportes de la Teoría Crítica para estudiar la enseñanza y el aprendizaje de la Plástica en la escuela. Esto permite hacer consideraciones respecto a recurrencias en las prácticas escolares relacionadas con el lenguaje visual, en especial sobre la escisión entre conocimiento y praxis.","palabras_clave":"ARTES PLÁSTICAS|EDUCACIÓN|TEORÍA CRÍTICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60320,"idioma_id":5,"titulo":"Prácticas de enseñanza-aprendizaje del Arte Impreso relacionadas con la incorporación de herramientas digitales en la formación de los estudiantes","resumen":"En  este artículo analizo prácticas y discursos de profesores y alumnos  relacionados con la incorporación de recursos digitales para la producción de  impresos. Este estudio se desarrolla en el marco de la formación de grado en la  Facultad de Bellas Artes de La Plata. Expongo cuál es el aporte de una  perspectiva constructivista en la educación superior y cómo repercute en el  encuentro entre tradición e innovación dentro de la Academia.","palabras_clave":"IMPRESOS|HERRAMIENTAS DIGITALES|CONSTRUCTIVISMO|UNIVERSIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60321,"idioma_id":5,"titulo":"La producción de impresos en la formación de grado.","resumen":"El trabajo analiza la inclusión de recursos digitales en los procesos de enseñanza-aprendizaje  del Taller de Grabado y Arte Impreso Básico de la Facultad de Bellas  Artes (UNLP). El trabajo de campo incluyó el registro de las prácticas áulicas y la realización de entrevistas a docentes y a estudiantes de los cuatro niveles anuales y de la instancia de elaboración de tesis en el caso de la Licenciatura.Se presentan en este artículo los resultados  parciales alcanzados y la interpretación  de los rasgos regulares y discontinuos del  universo de estudio, sobre la base del material obtenido en la observación participante de clases.","palabras_clave":"IMPRESOS|PRÁCTICAS|DISCURSOS|PROCEDIMIENTOS DIGITALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60436,"idioma_id":5,"titulo":"Cálculos de la Energía Generada y la Mitigación de Emisiones de Gases en Instalaciones para Calentamiento Solar de Agua?-","resumen":"xx","palabras_clave":"Energía solar|Colectores solares|Medio ambiente"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60438,"idioma_id":5,"titulo":"Caracterización geofisica de la Cuenca de Quehué, provincia de La Pampa","resumen":"RESUMENLa cuenca de Quehué se reconoce como una faja continua de valores negativos de anomalías gravimétricas de Bouguer, muy estrechay elongada, que se ubica en el este de la provincia de la Pampa. Se desarrolla a lo largo de unos 300 km con una orientación N35ºO,desde Luan Toro al norte, pasando por Quehué y Abramo, hasta la Laguna Colorada Grande al sur. En base a los resultados geofísicos,se considera que está limitada por importantes fallas y que se trata de una cuenca tipo rift similar a la de General Levalle. Se consideraque esta cuenca forma parte del sistema de rifts pampeanos de la Argentina, en conjunto con la cuenca de Macachín con la que serelaciona genéticamente. En el área comprendida en esta investigación se observan máximos y mínimos gravimétricos relativos de diferentemagnitud que se asocian con cambios en la profundidad del basamento. En la cuenca se reconocen seis depocentros que varíanen extensión y espesor sedimentario. Aunque sólo se ha confirmado la presencia de sedimentitas cretácicas, es posible que el rellenoinicial de la cuenca haya comenzado en el jurásico y esté apoyado, en parte, sobre sedimentos paleozoicos. Las variaciones en los datosgravimétricos y magnéticos a ambos lados de la cuenca señalan diferencias en la composición del basamento.","palabras_clave":"PROSPECCION GEOFISICA|DEPOCENTROS|CUENCA DE QUEHUE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60473,"idioma_id":5,"titulo":"Heterogeneidad estructural: origen y evolución del concepto frente a los nuevos desafíos en el contexto de la mundialización del capital","resumen":"El objetivo de este trabajo es analizar la evolución del pensamiento estructuralista latinoamericano a la luz de los cambios en la internacionalización del capital. Los pioneros de la escuela estructuralista, incluyendo los precursores del pensamiento de la CEPAL, analizaron las implicancias del carácter especializado y heterogéneo de una  estructura económica periférica como la de Argentina en el contexto de la configuración de la economía mundial de la segunda postguerra. De acuerdo a estos autores, el desarrollo económico estaba limitado por una la inserción tardía y dependiente en el sistema global de centro-periferia. Esto se expresaba en la famosa ley sobre la tendencia a la disminución de los términos de intercambio y en la reproducción persistente de la heterogeneidad estructural. A partir de los años ´70 ciertos autores estructuralistas prestaron atención a las estrategias de las Corporaciones Multinacionales como determinante principal de las asimetrías estructurales durante la industrialización de posguerra. Esta línea de investigación, que combina las limitaciones estructurales para el desarrollo y los procesos de concentración\/descentralización ha sido descuidada a partir de los años ´80. Desde finales de los años ´90 el enfoque de cadenas globales de valor ha devenido en el centro de interés de varios estudios sobre el desarrollo, sin embargo no ha sido analizado con una perspectiva centro-periferia estructural.","palabras_clave":"Heterogeneidad estructural|Estructuralismo|Cadenas globales de valor|Internacionalización productiva|Subdesarrollo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60479,"idioma_id":5,"titulo":"Jorge Abelardo Ramos, el inventor de Ugarte. Marginalidad, canon y nación.","resumen":"En  el presente trabajo pretendemos indagar la manera como ciertas lecturas  resultan interesadas y están motivadas en necesidades que emergen del  presente mismo de la lectura. Estas políticas de lecturas recuperan o ignoran  obras y autores dentro del canon cultural de una nación. Jorge Abelardo Ramos  editó por primera vez a Manuel Ugarte en la Argentina un libro que databa de  1910 El porvenir de la América Latina.  Le introduce un prólogo que lo llama ?Redescubrimiento de Ugarte?. La figura de  este socialista argentino le sirvió a Ramos para alcanzar una síntesis entre  socialismo y nación latinoamericana.","palabras_clave":"ensayo latinoamericano|manuel ugarte|escritores marginales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60480,"idioma_id":5,"titulo":"TARJA (JUJUY, 1955-1960): LA CULTURA DE LOS BORDES.","resumen":"Con este trabajo pretendemos poner de relieve la conciencia de la marginalidad que experimentan los miembros de una publicación literaria ?Tarja- de una provincia alejada como Jujuy. Pero para que la distancia se haga perceptible debe existir un punto cero o ?meridiano de Greenwich? desde  donde establecer las diferencias. Ese punto es la ciudad portuaria de Buenos Aires. Las observaciones que registra la revista sobre estos tópicos abre asimismo otra variable: la arbitrariedad de la ?provincia? y la relevancia histórico-cultural de la pertenencia a una región. En nuestro caso, una región andina transprovincial y supranacional.","palabras_clave":"revistas literarias,   |meridiano cultural|Buenos Aires-Interior|región"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60505,"idioma_id":4,"titulo":"Simulation of Spread of Infectious Diseases and Population Mobility in a Deterministic Epidemic Patch Model","resumen":"  Abstract: Computer simulation models are widely applied in various areas of the health care sector, including the spread of  infectious diseases. Patch models involve explicit movements of people between distinct locations. The aim of the present work has  been designed and explored a patch model with population mobility between different patches and between each patch and an  external population. The authors considered a SIR (susceptible-infected-recovered) scheme. The model was explored by computer  simulations. The results show how endemic levels are reached in all patches of the system. Furthermore, the performed explorations  suggest that the people mobility between patches, the immigration from outside the system and the infection rate in each patch, are  factors that may influence the dynamics of epidemics and should be considered in health policy planning.","palabras_clave":"SIMULATION|SPREAD OF INFECTIOUS DISEASES|POPULATION MOBILITY|EPIDEMIC PATCH MODEL|SIR MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60512,"idioma_id":5,"titulo":"El movimiento sindical argentino y sus relaciones internacionales: una contribución sobre la presencia de la CIOSL y la ORIT en la Argentina desde fines de los ´40 hasta comienzos de los ´80","resumen":"El presente artículo aborda untema escasamente analizado en la historiografía, el cual sin embargo reviste graninterés e importancia: la presencia e impacto de la corriente del sindicalismo'libre' en la Argentina, a partir del análisis de la influencia yconexiones de la CIOSL y su organización regional ORIT desde fines de los años´40 hasta comienzos de los años ´80. En primer lugar, se incluye una brevecontextualización histórica e historiográfica sobre la CIOSL y la ORIT. Ensegundo lugar, se presenta una cronología tentativa de la relación entre laCIOSL\/ORIT y el movimiento sindical argentino, así como un estudio sintético,basado en el trabajo de archivo en curso, de las características ytransformaciones de esta relación en cada uno de los sub-períodosidentificados. Finalmente, el trabajo se cierra con algunas conclusionespreliminares.","palabras_clave":"HISTORIA DEL TRABAJO EN AMERICA LATINA|MOVIMIENTO SINDICAL ARGENTINO|RELACIONES INTERNACIONALES|CIOSL-ORIT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60577,"idioma_id":4,"titulo":"High performance PbS Quantum Dot Sensitized Solar Cells exceeding 4% efficiency: the role of metal precursors in the electron injection and charge separation","resumen":"Here we report the preparation of high performance Q4 Quantum Dot Solar Cells (QDSCs) based on PbS?CdS  co-sensitized nanoporous TiO2 electrodes. QDs were directly grown on the TiO2 mesostructure by the  Successive Ionic Layer Absorption and Reaction (SILAR) technique. This method is characterized by a fast  deposition rate which involves random crystal growth and poor control of the defect states and lattice  mismatch in the QDs limiting the quality of the electrodes for photovoltaic applications. In this work we  demonstrate that the nature of the metallic precursor selected for SILAR has an active role in both the  QD?s deposition rate and the defect?s distribution in the material, with important consequences for the  final photovoltaic performance of the device. For this purpose, acetate and nitrate salts were selected as  metallic precursors for the SILAR deposition and films with similar absorption properties and consequently  with similar density of photogenerated carriers were studied. Under these conditions, ultrafast carrier  dynamics and surface photovoltage spectroscopy reveal that the use of acetate precursors leads to higher  injection e&#64259;ciency and lower internal recombination due to contribution from defect states. This was  corroborated in a complete cell configuration with films sensitized with acetate precursors, achieving  unprecedented photocurrents of B22 mA cm2  and high power conversion e&#64259;ciency exceeding 4%,  under full 1 sun illumination.","palabras_clave":"Quantum Dot Solar Cells|PbS\u2013CdS|nanoporous TiO2 electrodes.|Successive Ionic Layer Absorption and Reaction (SILAR)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60591,"idioma_id":4,"titulo":"SVD inversion for the bi-dimensional Conical Radon Transform","resumen":"In nuclear medicine, the Conical Radon Transforms family includes 2D and 3D models for scatter radiation imaging. The underlying idea is taking advantage of Comptom e\u000Bffect in order to provide image reconstruction without rotating the detector system. Several techniques currently applied for the classical Radon transform may also be useful in this new context. Nevertheless, since the physical model is rather di\u000Bfferent, it is necessary to assess the performance of each method. In previous works, we have studied inversion by Filtered-backprojection and adaptive algebraic reconstruction techniques. In this paper we study the performance of a method based in the factorization of the projection matrix in order to invert the projections. Particularly, we test our algorithms on the bi-dimensional Conical Radon Transform, a simple member of the Conical Radon family. We obtain useful results on the performance of the three methods which should be valuable in the more realistic 3D context.","palabras_clave":"Bi-dimensional Conical Radon Transform|Singular Value Decomposition|Gamma-ray Imaging"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60639,"idioma_id":5,"titulo":"EVALUACIÓN DE SERVICIOS DE POSICIONAMIENTO POR INTERNET EN  ARGENTINA","resumen":"Abstract  Online GPS (Global Positioning System) positioning services provide to the users the chance to obtain coordinates of centimeter quality by means of one single or double frequency GPS receiver, simplifying the logistic of the field work.  In the present work, the analysis of the results of three online positioning services is shown: IBGE-PPP, AUSPOS and CSRS-PPP. While AUSPOS applies a differential positioning method, using the IGS stations close to the study area, the resulting coordinates of IBGE-PPP and CSRS-PPP come from Precise Point Positioning (PPP).  The evaluation was carried out on the GPS coordinates of four stations that belong to the Red Argentina de Monitoreo Continuo (RAMSAC): LPGS, MECO, UNSA and RIO2. The solution SIR11P01 (epoch 2005.0), which is aligned to ITRF2008, epoch 2005.0, provides coordinates and velocities to these stations.  In order to realize this work, different packages of data of different time interval (20 min, 1, 3, 6 and 12 hours) corresponding to different epochs and observational conditions were sent with the aim of comparing the results obtained after the use of rapid and precise orbits, among others, offered by the online processing services.  After obtained the solutions returned by each service expressed in the ITRF2008 frame and in the observation epoch, we proceed to compare these results with those coordinates obtained from SIR11P01 in the same epoch. This allowed us to conclude that AUSPOS showed the major stability in terms of errors variability, while the best results were shown by CSRS. The r.m.s determined on the coordinates was, in some cases, better than 5 cm.   Keywords: online positioning processing services, PPP, SIRGAS","palabras_clave":"ONLINE POSITIONING PROCESSING SERVICES|PPP|SIRGAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60660,"idioma_id":5,"titulo":"Las conclusiones del VI Congreso Nacional de Práctica Profesional","resumen":"os días 8 Y 9 de noviembre de 2012, se celebró en nuestra Facultad de Derecho de la Universidad de Buenos Aires el VI Congreso Nacional de Práctica Profesional, bajo el lema: 'Violencia Social: Nuevos desafíos desde la práctica profesional'.-     Participaron del encuentro, ponentes y asistentes de Argentina, Colombia y Uruguay, así como conferencistas y destacadas personalidades.-  Con posterioridad, y en el marco del Congreso, se realizó video conferencia sobre el tema: 'La violencia en el ámbito internacional: Experiencias en Argentina y España', con la participación de quien escribe estas líneas, y de la Dra. Olga Edda Ciancia por la Facultad de Derecho de la UBA, y de los Dres. Lorenzo Mateo Bujosa Vadell, Marta del Pozo Pérez, Federico Bueno de Mata, así como Laura Gallego Herráez, por la Facultad de Derecho de la Universidad de Salamanca, España.-El encuentro fue fecundo, con un público heterogéneo, y gran participación del alumnado. También se entregaron premios a las mejores ponencias, las que próximamente serán publicadas en esta columna.-  Acercamos hoy a nuestros lectores las conclusiones de las comisiones de trabajo del Congreso, esperando sean de utilidad para el análisis y reflexión de las cuestiones planteadas.-","palabras_clave":"CONGRESO |UBA|2012|VALLET"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60670,"idioma_id":5,"titulo":"Astilleros estatales e industria naval argentina: trayectoria reciente y desafíos futuros.","resumen":"El objetivo de este artículo es analizar la evolución reciente de la industria naval pesada en la Argentina y de los astilleros estatales (Astillero Río Santiago, ARS, Talleres Navales Dársena Norte y Tandanor) que explican, en gran medida, la historia del sector. La hipótesis de trabajo que proponemos en estas páginas es que la industria naval pesada argentina y los astilleros estatales habrían demostrado una significativa dependencia de la capacidad del Estado para ejercer un papel integral como regulador, productor, demandante y proveedor de financiamiento. Desde el punto de vista conceptual, nuestro interés es el de contribuir a un debate más amplio sobre la relación entre las funciones del estado y el proceso de industrialización. Desde el punto de vista empírico, nos proponemos identificar y explicar este vínculo a través de la trayectoria de un rama industrial.  La convicción del fracaso del Estado en tanto promotor del desarrollo y el crecimiento sustentó al paradigma neoutilitarista del Estado mínimo que operó, fundamentalmente, en  la Argentina de los ?90. Se adaptaron, entonces las recetas económicas ortodoxas sobre el manejo del ajuste estructural. Las consecuencias de estas políticas en la industria naval pesada condujeron a una sistemática contracción de la fabricación de buques y artefactos navales, provocando el cierre de astilleros y la casi desaparición de la industria naval-partista. Desde el inicio del siglo XXI, los cambios en el rumbo de las políticas de estado generaron las condiciones de posibilidad para la recuperación de los astilleros públicos.  Palabras claves: Estado, industria naval pesada,  astilleros estatales.","palabras_clave":"estado|industria naval|astilleros estatales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60820,"idioma_id":5,"titulo":"¿Gobernar con el enemigo? Los radicales santafesinos en los inicios de la democracia electoral. Santa Fe, 1912-1916","resumen":"En el marco definido por la reforma política de 1912, el análisis de la experiencia gubernamental del radicalismo santafesino hace que nos propongamos indagar las condiciones que hicieron de la disidencia su acompañante permanente. El interrogante invita al análisis del radicalismo hacia fuera y hacia dentro de la Unión Cívica Radical de Santa Fe, durante su primera gestión de gobierno (1912-1916). Pero también amerita colocar sobre el tapete los vínculos que aquella organización mantuvo con la Unión Cívica Radical, en el pasaje de  partido de oposición -con un pasado revolucionario- a partido de gobierno. En los límites de estos planteos, los radicales santafesinos esgrimieron un pasado reciente en el que la violencia revolucionaria había dado lugar a la definición de una identidad política que se proponía, en coincidencia con otras voces, la regeneración de la política. Consideramos que una de las hipótesis que podría ordenar el recorrido de la historia santafesina durante estos años se orienta hacia los lazos que unían y, al mismo tiempo, enfrentaban a los radicales hasta el extremo en que el adversario -perteneciente al radicalismo u otra organización política- era visto como un oponente ilegítimo.","palabras_clave":"UNIÓN CÍVICA RADICAL|UNIÓN CÍVICA RADICAL DE SANTA FE|FACCIONES|ELECCIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60865,"idioma_id":4,"titulo":"Self-healing mortars based on glass hollow tubes and epoxy-amine systems.","resumen":"In the present work key parameters of different epoxy systems (such as viscosity and gel time) were evaluated to be used as healing agents when were included in a cement matrix. Epoxy systems were encapsulated in hollow glass tubes and were introduced in a mortar matrix. Samples were preloaded under three point bending in order to create a crack and release the healing system. After that, they were loaded to measure the residual strength and estimate the healing efficiency. The influence of temperature and the volume of the glass tubes were examined. Regarding the healing efficiency, a higher temperature led to an improvement of autogenous healing of the mortar matrix and a higher degree of crosslinking of the healing agent. For the studied systems, the use of glass tubes with smaller diameter containing the healing system seemed to be better in order to maintain the mechanical properties of the mortarbased composite.","palabras_clave":"SELFHEALING|HOLLOW GLASS TUBE |EPOXY SYSTEM|REPAIR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60873,"idioma_id":5,"titulo":"Hacia la segmentación significativa","resumen":"ResumenLa segmentación es uno de los tres pilares del marketing. Como tal su importancia es vital, a la hora de que las entidades y empresas de todo tipo se quieran dirigir a sus respectivos mercados. Ahora, un punto esencial es que las organizaciones no deberían elegir de manera unilateral a sus segmentos de mercado meta. Muy por el contrario el proceso, en realidad, se produce justamente al revés. Es decir, son los consumidores quienes se auto-segmentan antes de que la organización trabaje en ello. Quiere decir, que lo que las entidades y empresas deben hacer un esfuerzo mayor por identificar a esos segmentos ya definidos, y para ello la metodología tradicionalmente utilizada basada en las bases de segmentación resulta ser una extrema simplificación del problema en cuestión. Ahora bien, cuando escribimos la Segunda Miopía del Marketing (París, 2013), obra en la cual anunciamos que los consumidores no saben lo que compran, y también denunciamos que la gran mayoría de las empresas tampoco saben lo que venden; tuvimos que desarrollar las técnicas de afloramiento de significados, a los fines de identificar lo que realmente compra el consumidor, que como sabemos es la representación psíquica de la cosa, que hemos llamado ?significado?. Y al hacerlo y como una necesidad de la misma metodología, diseñamos un proceso de segmentación. Sin embargo, en la Segunda Miopía del Marketing, no dimos detalles precisos acerca de todos los aspectos teóricos que acompañan a este procedimiento. Por esta razón surgió la necesidad de ampliar estos temas a través de este nuevo método que hemos denominado Segmentación Significativa en el cual presentamos el procedimiento que hemos desarrollado para su aplicación en la identificación más precisa de los segmentos de mercado. Estos segmentos serán mucho más homogéneos porque todos sus integrantes comparten la cuestión más esencial con respecto a su producto, servicio o idea: un mismo significado, que es la base conceptual de todo plan de marketing esencial.","palabras_clave":"Segmentación|Segmento|Significados|Mercado"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":60948,"idioma_id":4,"titulo":"On Geastrum argentinum, a forgotten species","resumen":"Geastrum argentinum, a long forgotten species described by Spegazzini and previouslyregarded by Soto and Wright (2000) as a synonym of G. fimbriatum, has been found inseveral locations in Argentina. A careful analysis revealed relevant differences betweenthose taxa, proving the validity of Spegazzini?s species. Here we re-describe Spegazzini?sspecies from the damaged type material and from new fresh collections, proposing one ofthem as an epitype.","palabras_clave":"BASIDIOMYCOTA|EPITYPIFICACION|GEASTRACEAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61002,"idioma_id":4,"titulo":"A new species of the subgenus Forcipomyia (Phytohelea) from Argentina (Diptera: Ceratopogonidae)","resumen":"The adult and pupa of a  new species of Forcipomyia (Phytohelea) are described, illustrated  and photographed. Pupae were collected from water held by Eryngium L. sp (Apiaceae)  in Corrientes province, Argentina and reared to adults in the laboratory. Adult  and pupa are compared with the closely related congener F. (P.) jocosa Saunders, and the pupa is also  compared with that of F. (P.) bromelicola  (Lutz).","palabras_clave":"NEW SPECIES|TAXONOMY|PHYTOTELMATA|ERYNGIUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61061,"idioma_id":5,"titulo":"Consenso para la Enfermedad de Gaucher:  Grupo Argentino de diagnóstico y tratamiento de la Enfermedad de Gaucher","resumen":"Gaucher disease is the most common lysosomal storage disease. It is a multisystem chronic and progressive disorder. The determination of a decreased acid ß-GA is mandatory for the diagnosis and it is essential before initiating enzyme replacement therapy (ERT).The presence of Gaucher cells in the liver, lymph nodes and bone marrow biopsy specimens guides to the diagnosis but does not replace the determination of the enzyme activity. From the analysis of previous published papers we conclude that enzyme replacement therapy with Imiglucerase is the current standard therapy which has significantly improved the quality of life of patients with type I Gaucher disease. Treatment of Gaucher disease is life-long. The establishment of therapeutic goals encourage patients to adhere to treatment and minimizes interruptions. Achievement of therapeutic goals serves as a marker to evaluate the consequences of changes or adjustments in the therapeutic regimen. The dose adjustment or the frequency of the administration only should be considered when the most relevant therapeutic goals have been achieved , which should be maintained with each modification of the therapeutic regimen. The lost of any of the therapeutic goals indicates a failure of the treatment modification, although it should be considered the possibility of an intercurrent condition which could confound the analysis of the evolution of the disease. The individualized treatment of Gaucher I patients with ERT under the guidance of an experienced multidisciplinary team, specialized in metabolic diseases is the most efficient and cost-effective method to improve the health of patients with Gaucher I disease. Nevertheless it is difficult to integrate the updated scientific information available, related to diagnostic techniques, initial dose, maintenance, and treatment suspension. This can give rise to contradictory conclusions, variations in clinical practise , inadequate treatments and less efficient cost-effective practises. For these reasons it becomes necessary to organize and study critically the available information , in order to be able to generate recommendations in agreement with the scientific evidence and with the national facts in the managements of this pathologies. The present work represents the evaluation of the updated scientific information, which will be revised, analysed and amplified periodically.","palabras_clave":"ENFERMEDAD DE GAUCHER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61162,"idioma_id":5,"titulo":"Los Daños causados y sufridos por menores (tercera parte): Derecho a la salud de niños, niñas y jóvenes. Responsabilidad parental y responsabilidad del Estado","resumen":"Daños causados y sufridos por menores (tercera parte): Derecho a la salud de niños, niñas y jóvenes. Responsabilidad parental y responsabilidad del Estado.","palabras_clave":"Daños|Menores|Responsabilidad parental"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61376,"idioma_id":5,"titulo":"Traidores de textos: volver a leer con los oídos","resumen":"A lo largo de este artículo, me propongo desarrollar dos ideas, ambas vinculadas a la aún tímida puesta en valor de las prácticas de lectura en voz alta y de narración oral en la escuela. Por un lado, argumento a favor de la revalorización del cuerpo de quien, como  na ?celestina?, media  entre  la  letra  escrita  y  los  oyentes, se  apropia  de  un texto  ajeno,  lo modifica  con su  voz,  con silencios  y  con  gestos;  y  por  otro, sostengo  que  estas  prácticas pueden constituir una renovada vía de acceso a la literatura. ","palabras_clave":"NARRACIÓN ORAL|ENSEÑANZA DE LA LITERATURA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61490,"idioma_id":5,"titulo":"Analisis Del Rendimiento En Cereales De Invierno Mediante Mapas De Rendimiento Y (NDVI) En El Sudoeste Bonaerense","resumen":"RESUMENLos sistemas de posicionamiento global (GPS) combinados con monitores de rendimiento (MOR) proporcionan la oportunidad de mapear los rendimientos durante la cosecha. Las imágenes provenientes del sensor MODIS (MOD 13 Q1) podrían ser utilizadas en agricultura de precisión(AP). Resulta interesante evaluar las relaciones existentes entre los mapas de rendimiento (MR) y el Índice de Vegetación de Diferencia Normalizada (NDVI)en cereales de invierno en el Sudoeste Bonaerense (SOB), con la intención de aportar datos experimentales iniciales que permitan investigaciones futuras aplicadas a la AP. Para ello se planteó como objetivos:a) Realizar el filtrado de los datos provenientes de cosechadoras equipadas con GPS y MOR para obtener MR; b) Determinar NDVI para fechas próximas a floración en trigo y cebada en el área experimental; c) Identificaráreas con variaciones en el rendimiento con datos provenientes de la campaña de trigo y cebada 2010; d) Correlacionar los MR y las imágenes NDVI. El ensayo se realizó en dos lotes ubicados en el partido de Coronel Dorrego. Se utilizaron cultivos de trigo para pan (Triticumaestivum) y cebada cervecera (Hordeumdistichum) bajo el sistema de siembra directa. Se encontró una regresión significativa entre los valores de MR y el NDVI con un r2 de 0,84 y 0,75, respectivamente, de forma que en situaciones que no exista disponibilidad de MR podrían utilizarse los NDVI para suplantarlas. Se lograron identificar cinco zonas con variaciones importantes del rendimiento en trigo y cebada en el área experimental.","palabras_clave":"AGRICULTURA DE PRECISION |MONITORES DE RENDIMIENTO|SENSORES MODIS|TRIGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61550,"idioma_id":4,"titulo":"Cranial and dental studies of Glossotherium robustum (Owen, 1842) (Xenarthra: Pilosa: Mylodontidae) from the Pleistocene of southern Brazil","resumen":"Mylodontine ground sloths were studied from several Pleistocene localities of Rio Grande do Sul State: Sa?o Gabriel (central region), Quara&#305;´, Uruguaiana and Alegrete (western region) and Santa Vito´ ria do Palmar (coastal region) municipalities. The cranial and mandibular material is assigned to Glossotherium robustum based on the enlargement of the anterior portion of the maxilla, a rounded and ventrolaterally arched fossa for the estylohyal, an elliptical occipital condyle, a spatulate symphyseal region of the mandible, together with the size and degree of lobulation of the teeth. Comparison with specimens from the Pampean region of Argentina, western Uruguay and northeastern Brazil  revealed that the Rio Grande do Sul material is most similar morphologically to that of the Pampean region of Argentina and Uruguay. These southern specimens are morphologically distinct from Pleistocene material from tropical Brazil assigned to Glossotherium sp. The Pleistocene records of G. robustum indicate that this taxon was widely distributed between 20°S and 40°S spanning Argentina, Brazil, Bolivia, Chile,Uruguay and Paraguay, whereas Glossotherium sp. was restricted to latitudes 30°S.","palabras_clave":"GLOSSOTHERIUM|PLEISTOCENE|RIO GRANDE|BRAZIL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61608,"idioma_id":5,"titulo":"Las creencias epistemológicas como factores que afectan la formación de los ingenieros","resumen":"El propósito de este trabajo fue caracterizar los supuestos epistemológicos y las modalidades de pensamiento que despliegan los alumnos de las carreras de ingeniería al desarrollar su actividad curricular. Se tuvo en cuenta que los alumnos de ingeniería interactúan con el conocimiento desde sus creencias epistemológicas, las que les deberían permitir alcanzar las competencias que exige la actividad profesional. Esto tiene implicancias relevantes para su proceso de formación, pues afectan la concepción de aprendizaje en ciencias y definen la habilidad de comprender, trabajar y construir modelos de procesos y situaciones. Esto implica interpretar, construir y usar representaciones de los hechos, cuyo estudio se inicia con las ciencias básicas. Este trabajo sostiene que las creencias epistemológicas son constructos y el resultado de condicionantes. Para caracterizar estos constructos se utiliza una prueba exploratoria en todos los alumnos del primero y último año de la Facultad Regional Mendoza de la UTN, en carreras de ingeniería. Los datos fueron procesados estadísticamente y revelaron que en los alumnos predomina una concepción dualista de la ciencia (Positivismo \/ Constructivismo), lo que sugiere que hay una deficiencia en la formación del concepto de ciencia y de investigación científica tecnológica.&nbsp; &nbsp; &nbsp; &nbsp; ","palabras_clave":"SUPUESTOS EPISTEMOLÓGICOS|ALUMNOS DE INGENIERÍA|PROCESO DE FORMACIÓN |CONCEPCIÓN DUALISTA DE LA CIENCIA: POSITIVISMO \/ CONSTRUCTIVISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61659,"idioma_id":5,"titulo":"Entre la educación y el trabajo: experiencias formativas de jóvenes trabajadores","resumen":"En este artículo nos acercaremos a las experiencias formativas vinculadas al trabajo de jóvenes que se desempeñan en una cadena multinacional de supermercados en la ciudad de Rosario. Este texto se enmarca en una investigación socio-antropológica más amplia sobre las relaciones laborales y las experiencias en torno al trabajo de estos jóvenes trabajadores de supermercados. En esta oportunidad nos centraremos en los procesos de formación y capacitación en el lugar de trabajo y cómo son valorados y apropiados por los trabajadores, para luego indagar en las experiencias educativas formales y su relación con el trabajo.","palabras_clave":"EXPERIENCIAS FORMATIVAS|JOVENES|TRABAJO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61802,"idioma_id":5,"titulo":"Medias de Presión Arterial en una Población de Niños Prematuros de Bajo Peso en Controles Ambulatorios","resumen":"Introduction: se encuentran pocos estudios de presion arterial (PA) en Recien Nacidos Pretermino Alto Riesgo. Es practica poco habitual tomarles la PA. Sigue siendo una incognita el comportamiento de la PA de los RNPTAR.  Objetivos: definir medias de PAS y PAD, prevalencia de HTA, en RNPTAR controlados desde el alta de UCIN hasta los 5 anos. Comparar con grupo control de ninos RNT. Analizar asociacion de la PA con factores de riesgo.  Material: poblacion. Inclusion: RNPTAR ( de 1 a 6 anos y GC. Los grupos etareos se eligieron segun edades de tablas de PA referentes. 2 G controles de RNT (termino), igual edad y sexo. Variables: sexo, edad gestacional (EG), edad corregida (EC), peso y talla de nacimiento (PN, TN); peso y talla al momento del registro, PAS y PAD(mmHg).  Metodo: tensiometro oscilometrico automatico, media de tres registros. Mangos adecuados. Curvas referentes para el G1 Second Task Force (TF) y para G2, 4a TF. Adiestramiento de enfermeras. Monitoreo. Consentimiento de padres. Aprobacion del Comite Hospital de Ninos. Diseno de corte transversal. Analisis descriptivo, asociacion y comparacion (test t y Pearson). Medias de PA ± 3 DS.  Resultados: 448 RNPT y 65 controles. Grupo 1 (G1 n=271), promedios de edad al momento del registro 5,9 m (4,6; 6,4), de PAS 101 mmHg (97; 91 ).y PAD 54(51 ;56) .HTAS en 22%; HTAD en 23%. Las medias de PAS y PAD en ambos sexos, fueron semejantes a referentes. HTAS asocio a PN (p= 0,02); Grupo 2 (G2 n= 177) edad 29 m (28; 31); PAS 88 mmHg (85.91) y de PAD 66(64; 67). HTAS en 38% e HTAD en 61%. La media de PAS de varones y mujeres fue semejante a curvas referentes. PAD se situo sobre el P95 en mujeres, a partir del ano hasta los 2 anos y en varones desde el ano hasta los 3 anos. HTA no asocio con ninguna variable. Grupo 1 control (n=85) PAS 94(92,95) p<0,001 y PAD 60(58; 61) (p= 0.0005). Grupo 2 (n=44) PAS 102 (100; 103) p=0,54; PAD 65(61; 68) p=0,01. La prevalencia de HTA fue mayor en los 2 grupos de RNPT que en los controles (p=0,04, p=0,03).  Condusiones: se graficaron curvas de medias de PAS y PAD RNPTAR para edad y sexo que evidenciaron aumento de PAD para ambos sexos, por arriba del percentil 95, desde los 6 m hasta 3 anos en varones y desde los 12 m hasta 2 anos en mujeres. Alta prevalencia de HTA. Asociacion a PN. Se recomienda incorporar el control ambulatorio de PA en RNPT.  Palabras clave: Presion Arterial Neonatal, prematuros, dispositivo oscilometrico automatico.","palabras_clave":"Presión Arterial Neonatal|Prematuros|Dispositivo oscilométrico automático"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61826,"idioma_id":5,"titulo":"Investigar sobre cine silente en Latinoamérica","resumen":"      Texto introductorio del dossier 'Cine Silente Latinoamericano' que coordiné para la revista Imagofagia. Dicho dossier, que reúne diez ensayos a cargo de investigadores  provenientes de Argentina (Jorge Sala y Andrea Cuatrterolo), Bolivia (Rodrigo  Romero Zapata), Brasil (Flávia Cesarino Costa y Luciana Corrêa de Araújo),  Chile (Mónica Villarroel y Jorge Iturriaga), Colombia (Juan Sebastián Ospina León),  México (Ángel Miquel y Itzia Fernández Escareño) y Uruguay (Georgina Torello),  propone mostrar un panorama actual de los estudios académicos sobre cine en  Latinoamérica desde una perspectiva amplia e interdisciplinaria, que incluye  aproximaciones culturales e históricas al tema, lecturas político-sociales,  análisis textuales e intertextuales (con la fotografía, la literatura y la  prensa ilustrada, entre otras) y estudios sobre la recepción.  ","palabras_clave":"CINE SILENTE LATINOAMERICANO|HISTORIA DEL CINE|ESTETICA DEL CINE|CINE TRANSNACIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61892,"idioma_id":5,"titulo":"La retórica del humor como estrategia en la enseñanza de textos multiculturales","resumen":"La enseñanza de la literatura de lengua inglesa en un profesorado de inglés en Argentina requiere de técnicas pedagógicas comparativas, ya que el estudiante debe asimilar los procesos interculturales para poder entender una literatura extranjera. Por lo tanto, en las cátedras de literatura en nuestro profesorado en la Universidad Nacional de Mar del Plata partimos de una visión comparatista que implica aprovechar los conocimientos y tradiciones culturales que los alumnos ya poseen, y sobre ellos construir una competencia interpretativa a través de las convenciones, la retórica y los géneros propios de la tradición literaria en lengua inglesa. Para mayor complicación, una de las unidades de estudio del programa es la literatura de los llamados escritores ?latinos? de Estados Unidos. Como no existe la misma situación multicultural en nuestra región, hemos debido diseñar estrategias y técnicas de enseñanza apropiadas. Una de ellas es el taller de traducción. Otra, el análisis de la retórica del humor, en autores que lo usan como recurso estilístico.","palabras_clave":"HUMOR|COMPARADA|MULTICULTURAL|ENSEÑANZA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61919,"idioma_id":5,"titulo":"Vientos de cambio. Comentarios en torno al Proyecto de modificación del art. 34, inc. 1º del Código Penal Argentino.","resumen":"Desde antaño el diálogo entre médicos y juristas ha resultado arduo, complejo  y no exento de múltiples y variados equívocos. Esto ha permitido la  proliferación de opiniones e interpretaciones psiquiátrico-forenses sesgadas,  por momentos grotescas en términos de Foucault. En tal sentido, algunos de estos conceptos equívocos son, en el ámbito del  derecho penal, la alteración morbosa, la insuficiencia de las facultades mentales,  el estado de inconsciencia y la comprensión de la criminalidad del  acto. También surgen nuevos constructos como ?normalidad psicojurídica.  En las últimas décadas, diferentes países han encarado una reforma en  la redacción de las fórmulas de inimputabilidad, optando por conceptos  amplios, tales como anomalía o alteración psíquica. En esta línea se encuentran  los Códigos Penales de España, Alemania, y Francia, entre otros.  En nuestro medio, la propuesta de modificación en la redacción del art. 34,  inc 1°, que lleva varios años en estudio, se encuentra en consonancia no  sólo con los modernos Códigos Penales europeos y latinoamericanos, sino  también con las concepciones actuales sobre salud mental. Ya no se trata  sólo de psicosis o enajenación como sinónimo de enfermedad mental ?y,por ende, de alteración morbosa?, sino de un amplio concepto, que puede  incluir a los trastornos severos por consumo de sustancias, o trastornos  graves de la personalidad, e inclusive a los casos de retraso mental leve;  quedando este concepto amplio supeditado a la verificación de las limitaciones  que dichos cuadros tienen respecto del nivel de la comprensión  y dirección en un hecho puntual, pero sobre todo al análisis y valoración  jurídica de la capacidad de culpabilidad de ese sujeto, en un momento  puntual, para un hecho puntual.En caso de ser aprobada esta propuesta de modificación del art. 34, inc. 1°,  seremos testigos del nacimiento de una nueva etapa en la psicopsiquiatría  forense argentina.","palabras_clave":"Inimputabilidad por razones psiquiátricas|Psiquiatria Forense|Derecho penal|Código Penal"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61923,"idioma_id":4,"titulo":"Perinatal Outcomes of Multiple Gestation Pregnancies in Kenya, Zambia, Pakistan, India, Guatemala and Argentina: A Global Network Study","resumen":"Aim To determine the rates of multiple gestation, stillbirth, and perinatal and neonatal mortality and to determine health care system characteristics related to perinatal mortality of these pregnancies in low- and middle-income countries.Methods Pregnant women residing within defined geographic boundaries located in six countries were enrolled and followed to 42 days postpartum.Results Multiple gestations were 0.9% of births. Multiple gestations were more likely to deliver in a health care facility compared with singletons (70 and 66%, respectively, p < 0.001), to be attended by skilled health personnel (71 and 67%, p < 0.001), and to be delivered by cesarean (18 versus 9%, p < 0.001). Multiple-gestation fetuses had a relative risk (RR) for stillbirth of 2.65 (95% confidence interval [CI] 2.06, 3.41) and for perinatal mortality rate (PMR) a RR of 3.98 (95% CI 3.40, 4.65) relative to singletons (both p < 0.0001). Neither delivery in a health facility nor the cesarean delivery rate was associated with decreased PMR. Among multiple-gestation deliveries, physician-attended delivery relative to delivery by other health providers was associated with a decreased risk of perinatal mortality.Conclusions Multiple gestations contribute disproportionately to PMR in low-resource countries. Neither delivery in a health facility nor the cesarean delivery rate is associated with improved PMR.","palabras_clave":"Perinatal |Pregnancies"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":61997,"idioma_id":4,"titulo":"Cymbella gravida sp. nov. a new lacustrine taxon from Santa Cruz, Argentina","resumen":"This study presents a detailed morphological analysis using light and scanning electron microscopy of Cymbella gravida sp. nov. collected from the deep sediments of Laguna Potrok Aike, a maar lake in the province of Santa Cruz, Argentina. An attempt was made to describe its ecological preferences based on the diatom assemblages present in the sections where C. gravida was found. This newly proposed species is compared with two morphologically related taxa, C. terrafuegiana Krammer and C. cistula var. guarrerae Ferrario, both described from the same locality in Tierra del Fuego. Light and scanning electron microscope observations of the original material allowed the establishment of their correct taxonomy.","palabras_clave":"DIATOMS|FRESHWATER,|CYMBELLACEAE|PATAGONIA|TAXONOMY |SUBFOSSIL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62075,"idioma_id":5,"titulo":"La provisión de agua potable en la periferia del AMBA, Argentina","resumen":"El artículo analiza la problemática de la provisión de agua potable domiciliaria en territorios con escasa cobertura de las redes en el principal aglomerado urbano de la Argentina denominado Área Metropolitana de Buenos Aires. El mismo recupera los aportes de una investigación exploratoria que recurrió al estudio de caso y el análisis de fuentes primarias y secundarias. Principalmente centra la atención en la caracterización de los circuitos mercantiles que emergen ante la ausencia de las redes, comprendidos por la presencia de empresas formales e informales que realizan perforaciones e instalan equipos que permiten la explotación individual de los acuíferos por hogares que no acceden a las redes de agua y exigua regulación, control y fiscalización estatal.","palabras_clave":"AGUA POTABLE|REGULACIÓN|MERCADOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62225,"idioma_id":4,"titulo":"Seminal parameters, testosterone serum concentration and cryopreserved sperm function of Fallow Deer Dama dama in captivity","resumen":"Cervids have a seasonal reproduction so it is important to consider those changes in male reproductive physiology in order to improve the optimal moment to obtain and storage deer sperm in genetic resources banks. Fallow deer Dama dama was used as a biological model. The purpose was to study the concentration of testosterone in serum, the seasonally deer semen quality during the brama and post-brama, sperm morphology and functional parameters of cryopreserved fallow deer sperm. Seminal material was obtained by electroejaculation. Free testosterone was determined by the method of calculation from RIA and SHBG and total testosterone was obtained by the chemiluminescence method. Capacitation and acrosome reaction were evaluated by chlorotetracycline epifluorescence technique (CTC) and acrosome integrity and viability by Trypan blue stain\/DIC. Data was analyzed by ANOVA and Tukey Test (p <0.05). Capacitation with heparin at different incubation times showed that the highest capacitation percentage was obtained at 45 minutes incubation, showing CTC sperm patterns as bovine spermatozoa, and it was confirmed by acrosome reaction induction. Total testosterone was 3.59 ± 3.15 ng\/ml and free testosterone 682.75 ± 380.80 pmol\/l in brama period; in post-brama period total testosterone was 0.37 ± 0.50 ng\/ml and free testosterone 104.60 ± 106.63 pmol\/l. The mean of total sperm number per ejaculate was 897.75 ± 535.51 x106 sp in brama and 12.06 ± 6.6x106 sp in post-brama period, with a sperm motility percentage of 81.66 ± 16.81 % and 45.00 ± 42.23 %, respectively. The average size of sperm head of fallow deer was long (larger diameter) 12.10 ± 0.31 &#956;m and wide (smaller diameter) was 6.87 ± 0.12 &#956;m. This study contributes to understand seasonally sperm quality, hormone variation and sperm processes induction allowing advancing with the development of reproductive biotechnologies as a conservation strategy for threatened species of the Cervidae family.","palabras_clave":"Dama dama|Sperm|Seminal parameters|Cryopreservation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62293,"idioma_id":4,"titulo":"Model Adaptation for Real-Time Optimization in Energy Systems","resumen":"Real-time optimization (RTO) is widely used in industry to operate processes close to their maximum performance. The models used for RTO need to be adapted using real-time data to ensure feasibility of the model optimal inputs and convergence to the real plant optimal point. Heat and power systems are suitable for being optimized in real-time because of their fast dynamics and the benefits achievable by reacting to changes in power prices and steam demand. This work proposes a modifier adaptation strategy that exploits the structure of certain problems to make the adaptation faster and more reliable, which is proven to be particularly useful for heat and power systems. The adaptation is performed in the equations that predict efficiencies or performance of unit operations. By identifying the variables that modify each performance factor, the number of data sets needed for gradient correction is reduced. This makes the proposed strategy suitable for real-time optimization of processes with a large number of inputs. Two alternatives are proposed to implement the approach: gradient calculation by finite differences and quadratic regression using current and past data. The features and behavior of this approach are shown through two case studies: (i) a simple model with three processes, and (ii) a heat and power system of a sugar and ethanol plant. A comparison with other existent approaches shows a better performance in terms of operating cost and sensitivity to noise.","palabras_clave":"REAL TIME OPTIMIZATION|MODEL ADAPTATION|ENERGY SYSTEMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62302,"idioma_id":4,"titulo":"ANTIINFLAMMATORY ACTIVITY OF Geoffroea decorticans (CHAÑAR) FRUITS AND ARROPE","resumen":"Geoffroea decorticans constitutes an important component of the current diet of NOA communities, consumed directly or as a derivative product called ?arrope?. It is used in folk medicine for its emollients, balsamic, antitussive and expectorant activities. Previous fi ndings from our laboratory showed that the aqueous extract of chañar fruits and arrope have antinociceptive activity in animal models of pain. The purpose of this study was to evaluate the antiinfl ammatory activity of extracts (aqueous and ethanol) and ?arrope? orally administered at doses of 500 and 1000 mg\/ Kg. The antiinfl ammatory effect was evaluated by the method of carrageenan-induced paw edema in Wistar rats (250 g). Ibuprofen (100 mg\/kg) was used as a positive control. The results showed that both extracts and arrope reduced the edema within 4h post dosing. Rats pretreated with the aqueous extract signifi cantly decreased in a dose dependent manner, reaching maximum inhibition (100%) at 3 hours with a dose of 1000 mg\/Kg. The antiinfl ammatory action of the ethanolic extract and arrope were 20% and 30% respectively at a dose of 1000 mg\/Kg, at 3 hours after treatment. In conclusion, this study has shown that the aqueous extract of fruits of chañar has a signifi cant antiinfl ammatory effect, probably through inhibition of prostaglandins biosynthesis, which is the mechanism of action of nonsteroidal antiinfl ammatory drugs.","palabras_clave":"ANTIINFLAMMATORY ACTIVITY |GEOFFROEA DECORTICANS|FRUITS|ARROPE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62304,"idioma_id":4,"titulo":"PERICARP PHENYLPROPANOIDS: RESISTANCE FACTORS TO MAIZE EAR ROT CAUSED BY Fusarium verticillioides","resumen":"Fusarium verticillioides, the ethiological agent of maize ear rot, contaminates grains with fumonisins that are potentially toxic for humans and animals. The development of more resistant hybrids is the less expensive and more sustainable strategy to minimize fumonisin contamination. The aims of this work were: 1) to determine content of pericarp phenylpropanoids in 11 maize genotypes from INTA germplasm; 2) to evaluate if pericarp phenylpropanoids play a role in resistance against F. verticillioides. Eleven maize genotypes were grown in Pergamino for two years and inoculated with microconidial suspensions of P364 (a high fumonisin producer strain). Disease severity, fumonisin contamination and content of phenylpropanoids were evaluated in the grains during harvest .The pericarp of the maize genotypes contained trans-ferulic, cis-ferulic and p-coumaric (pCA) acids and fi ve diferulates (DFAs). The most abundant DFAs were 8,5´-DFA benzofuram, followed by 8,5´-DFA and 8,8´-DFA. Field resistant genotypes exhibited the highest contents of phenylpropanoids, which were associated with the lowest fumonisin accumulation (-0.61 > r >-0.90). High levels of pericarp phenylpropanoids proved to be a maize trait associated with lower disease severity and fumonisin accumulation.","palabras_clave":"FUSARIUM VERTICILLIODES|MAIZE EAR ROT|PERICARP PHENYLPROPANOIDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62324,"idioma_id":4,"titulo":"Working Paper n 1 :The Tragedy of Clientelism: Opting Children Out","resumen":"Governments in new democracies launch social policies with the purported goal of alleviating the effects of poverty among the most vulnerable households, usually low income families with children. However, because the politicians who decide on these policies seek to maximize political support and because children do not vote, this goal is often thwarted by the clientelistic distribution of social policies benefits. Based on the main Argentine household survey and on personal interviews with 120 brokers, this paper provides evidence of a clientelistic distortion introduced in the distribution of social policies benefits. The paper shows that brokers collect information on family size and age composition and allocate workfare programs discriminating against the most vulnerable household; that is, against those families with more children not old enough to vote.  (Actualmente bajo referato en el Journal of Law, Economics and Organization).","palabras_clave":"poverty|workfare programs|clientelism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62387,"idioma_id":5,"titulo":"Conocimiento y valoración de la vegetación espontánea por agricultores hortícolas de la plata. Su importancia para la conservación de la agrobiodiversidad","resumen":"Se evaluó el conocimiento y valoración que los  agricultores de la zona Hortícola de La Plata tienen sobre la vegetación  espontánea presente en sus fincas. Se realizaron entrevistas semi-estructuradas  y recorridas a campo en 27 fincas. Se relevó la riqueza de especies  espontáneas,&nbsp; sus posibles usos y  funciones ecológicas y el conocimiento y la actitud (positiva, neutra,  negativa) que los agricultores tienen hacia este componente de la  agrobiodiversidad. Se confirmó la existencia de una importante diversidad de  especies espontáneas en la región con una considerable cantidad de usos  apropiables. Los agricultores demostraron poseer un bajo conocimiento general  de las especies espontáneas aunque existieron diferencias entre ellos. Se  observó un mayor conocimiento de los usos apropiables directos (comestibles y  medicinales) que de las funciones ecológicas de las especies. Este conocimiento  y valoración estuvo relacionado con algunas características de los agricultores.  Se discute la influencia que este conocimiento y valoración de especies  espontáneas, tiene sobre la conservación de la agrobiodiversidad in situ. ","palabras_clave":"agroecologia|biodiversidad|diversidad cultural|agricultura sustentable"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62531,"idioma_id":4,"titulo":"Labour Law Violations in Chile","resumen":"This empirical contribution to the quantification of labour law violationuses micro survey data to examine compliance with workers? statutory entitlements to a minimum wage, working time limits, a written contract, and pension coverage over the period 1990?2009. One-third of workers were denied at least one of these entitlements, albeit with significant variations in the incidence of violations over time, across laws and by worker and firm characteristics. The authors? econometric analysis shows that compliance rates are lower for female, foreignborn, indigenous and less educated workers, and in smaller firms and agricultural regions. Further research, they argue, should focus on enforcement.","palabras_clave":"LABOR|COMPLIANCE|MINIMUM WAGE|CHILE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62589,"idioma_id":5,"titulo":"Comentario a Rubinelli, María Luisa (comp.) (2013), Nosotros los latinoamericanos. Identidad y diversidad. Homenaje a Arturo A. Roig","resumen":"Se trata de un comentario a la obra Nosotros los latinoamericanos. Identidad y diversidad. Homenaje a Arturo A. Roig de Maria Luisa Rubinelli. Arturo Andrés Roig se dio a la tarea de pensar sin que pueda encasillarse su obra en un territorio disciplinar determinado. Su labor como profesor, historiador y filósofo se encuentra articulada con sus elaboraciones teóricas en diversos ámbitos disciplinares: la historia de las ideas, la filosofía latinoamericana, la pedagogía universitaria. Su obra, sutil y rigurosa, se inserta en un contexto de debate acerca de la liberación que desemboca en un humanismo crítico y en la moral de la emergencia. Moviliza tradiciones, categorías y figuras simbólicas de la tradición filosófica europea y latinoamericana para esclarecer cuestiones filosóficas y metodológicas. Lee la tradición y se orienta a dilucidar la pregunta acerca de la cuestión de la emancipación humana, que conlleva la afirmación crítica del sujeto. Entregado a la tarea de pensar Arturo Roig propone, crea, entramados categoriales que devienen herramientas imprescindibles para todos aquellos que se interrogan y reflexionan sobre la identidad y diversidad de los latinoamericanos. Es por ello que en 2009 Horacio Cerutti Guldberg ha señalado, en su Filosofando y con el mazo dando, que si queremos enterarnos del estado en que se encuentra al día de hoy la vanguardia de reflexión filosófica nuestroamericanista, se impone reexaminar meticulosamente la obra de Arturo Andrés Roig. La diversidad de contribuciones que compila María Luisa Rubinelli constituye un aporte más a la tarea propuesta por Horacio Cerutti Guldberg. El libro cumple su objetivo: destaca el valor de la producción teórica de Arturo Andrés Roig, es por ello que resulta interesante para aquellos que se ocupan de estudiar su pensamiento. Contribuye a la reconstrucción de su contexto discursivo al ponerlo en diálogo con otros autores y tradiciones filosóficas, tal como ocurre en los artículos de Fernán Gustavo Carreras y Gloria Silvana Elías. Muestran, los trabajos, los textos de Roig en su productividad: no sólo lo que dicen sus escritos, sino lo que ha posibilitado su obra.","palabras_clave":"ARTURO ROIG|NOSOTROS|IDENTIDAD|DIVERSIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62622,"idioma_id":4,"titulo":"Epiphytism in a subtidal natural bed of Gracilaria gracilis of southwestern Atlantic coast (Chubut, Argentina)","resumen":"The diversity of epiphytes, their temporal abundance variation and the anatomical structure of host?epiphyte interfaces were studied in the agarophyte Gracilaria gracilis from a natural bed in Bahía Bustamante, Chubut Province, Argentina. Twenty-nine algal species were recorded as epiphytes during 2 years of monthly sampling (March 2006?February 2008). Total epiphyte density ranged between 0.037 ind. cm&#8722;2 (November 2006) and  39.37 ind. cm&#8722;2 (April 2007), with higher density values throughout the second sampling year. Ceramiales species were the most abundant epiphytes. The density of Ceramium rubrum ranged from 0.09 ind. cm&#8722;2 (52 % of the total amount) in September 2006 to 17.4 ind. cm&#8722;2(44.18 % of the total amount) in April 2007. Epiphyte infection was more dependent on spore recruitment and sporeling development, especially on thalli derived from fragmentation, than on seasonal environmental variations.  The different infections were analysed, taking into account the epiphytic attachment strength and invasiveness and the degree of damage inflicted on the host. Calothrix confervicola was one of the most abundant species. This epiphyte,weakly attached to the host surface, generated no host tissue damage. In contrast, C. rubrum, Polysiphonia abscissa and other Ceramiales were the species that caused more damage to the host because their rhizoids penetrated the cortical portion of the host thallus, sometimes reaching the medullary tissue. Some generalisations and characterisations of the different epiphyte groups in relation to their consequences to Gracilaria spp. are presented.","palabras_clave":"EPIPHYTISM|NATURAL BED|ULTRASTRUCTURE|GRACILARIA GRACILIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62623,"idioma_id":4,"titulo":"Human AQP1 is a constitutively open channel that closes by a membrane tension-mediated mechanism","resumen":"This work presents experimental results combined with model-dependent predictions regarding the osmotic-permeability regulation of human aquaporin 1 (hAQP1) expressed in Xenopus oocyte membranes. Membrane elastic properties were studied under fully controlled conditions to obtain a function that relates internal volume and pressure. This function was used to design a model in which osmotic permeability could be studied as a pressure-dependent variable. The model states that hAQP1 closes with membrane-tension increments. It is important to emphasize that the only parameter of the model is the initial osmotic permeability coefficient, which was obtained by model-dependent fitting. The model was contrasted with experimental records from emptied-out Xenopus laevis oocytes expressing hAQP1. Simulated results reproduce and predict volume changes in high-water-permeability membranes under hypoosmotic gradients of different magnitude, as well as under consecutive hypo- and hyperosmotic conditions. In all cases, the simulated permeability coefficients are similar to experimental values. Predicted pressure, volume, and permeability changes indicate that hAQP1 water channels can transit from a high-water-permeability state to a closed state. This behavior is reversible and occurs in a cooperative manner among monomers. We conclude that hAQP1 is a constitutively open channel that closes mediated by membrane-tension increments.","palabras_clave":"AQP1|Cellular volume|Membrane tension"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62624,"idioma_id":4,"titulo":"Molecular dynamics of water in the neighborhood of aquaporins","resumen":"Present knowledge obtained by molecular dynamics (MD) simulation studies regarding the dynamics of water, both in the vicinity of biological membranes and within the proteinaceous water channels, also known as aquaporins (AQPs), is reviewed. A brief general summary of the water models most extensively employed in MD simulations (SPC, SPC\/E, TIP3P, TIP4P), indicating their most relevant pros and cons, is likewise provided. Structural considerations of water are also discussed, based on different order parameters, which can be extracted from MD simulations as well as from experiments. Secondly, the behaviour of water in the neighbourhood of membranes by means of molecular dynamics simulations is addressed. Consequently, the comparison with previous experimental evidence is pointed out. In living cells, water is transported across the plasma membrane through the lipid bilayer and the aforementioned AQPs, which motivates this review to focus mostly on MD simulation studies of water within AQPs. Relevant contributions explaining peculiar properties ofthese channels are discussed, such as selectivity and gating. Water models used in these studies are also summarised. Finally, based on the information presented here, further MD studies are encouraged.","palabras_clave":"Aquaporins|Water Model|SPC\/E|Water Permeability|Molecular Dynamics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62625,"idioma_id":4,"titulo":"Critical bulk density for a Mollisol and a Vertisol using least limiting water range. Effect on early  wheat growth","resumen":".....","palabras_clave":"critical bulk density| least limiting water range | soil shear strength| wheat growth"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62626,"idioma_id":4,"titulo":"Land use intensity and cropping sequence effects on aggregate stabiltiy and C storage in a Vertisol and a Mollisol","resumen":"The relevant change in land use due mainly to the rapid expansion of soybean cropping towards areas traditionally occupied for livestock purposes or with native grasslands of South America may have negative consequences on soil organic carbon (SOC) storage and aggregate stability, although the effect may be different between soils with contrasting aggregation agents. The aim of our work was to assess the impact of the land use, measured as the intensification and\/or frequency of a given crop, on SOC storage and aggregate stability in two soils differing in their main agents of aggregation. The study was conducted in a Mollisol and a Vertisol of Argentina. Eleven cropped fields (agricultural and crop-pasture rotation) under no-tillage and one uncropped situation (pristine native grassland) were selected in each soil type. The fraction of annual time with plant cover (as a measure of the intensification in the land use) and the frequency of a given crop in the cropping sequence over a 6-year period were calculated. Undisturbed soil samples were collected from each soil at 0-5, 5-15 and 15-30 cm depths. The SOC stocks in equivalent soil mass were calculated using the native grassland as the baseline system. Aggregate stability was evaluated using a method that involved three pretreatments: fast wetting, stirring after prewetting and slow wetting. The intensification improved the aggregate stability in the Mollisol, whereas a low impact of land use on aggregate stability was recorded in the Vertisol. Overall, both the intensification sequence index and the soybean cropping frequency were the best indexes to evaluate the impact of land use on aggregate stability and SOC storage, mainly in the Mollisol. The stirring after prewetting pretreatment was mainly associated with SOC concentration in the Mollisol, appearing as a method with high potential capacity to discriminate land use in the Mollisol, in which the SOC is the main aggregation agent. In contrast, the slow wetting pretreatment was more appropriate to evaluate the impact of land use in the Vertisol. The approach used to evaluate the land use, which included agricultural lands, crop-pasture rotation and native grasslands, evaluated through indexes of occupation with plant cover, was more suitable for the Mollisol than for the Vertisol. This reveals that the evaluation of land use through several indexes should be based on the soil type","palabras_clave":"Aggregation agents|Soil carbon stocks|Intensification sequence index|Soybean cropping frecuency"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62627,"idioma_id":4,"titulo":"Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX adjuvant","resumen":"    The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been  attributed to insufficient capacity of the vaccines to induce opsonizing antibodies.  Types  of antigen, administration route and adjuvant used in a vaccine formulation  have been identified as critical factors for the development of opsonic  antibodies as well as other  immune mechanisms. The humoral immune response of heifers immunized with  a S. aureus CP5 whole cell vaccine  formulated with a traditional adjuvant and with ISCOMATRIXTM was  evaluated. Twenty  primigravid Holstein dairy heifers in the last  trimester of gestation were immunized either with a vaccine formulated with ISCOMATRIXTM, Al(OH)3, or saline solution (placebo). Immunization was  carried out 45 and 15 days before the expected calving date. Heifers vaccinated with S. aureus adjuvanted with ISCOMATRIXTM  responded with significantly higher levels of anti-bacterin and anti CP5  IgG and IgG2 in sera than animals in the Al(OH)3 or control groups. Animals in the ISCOMATRIXTM group responded with significantly higher anti-bacterin specific IgG in whey than animals in the Al(OH)3 and control groups, detected from the first week post calving until the  end of the trial. Sera from animals inoculated with S. aureus in ISCOMATRIXTM, obtained 7 days post partum, significantly increased  both the number of neutrophils ingesting bacteria and the number of bacteria being ingested by the neutrophils,  compared with sera obtained from heifers vaccinated with Al(OH)3 or non-vaccinated controls. These features indicate that  ISCOMS can enhance humoral immune response promoting opsonization and  potentially improve available whole cell vaccines for S. aureus bovine mastitis.  ","palabras_clave":"mastitis|Staphylococcus aureus|immunization|ISCOMATRIX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62628,"idioma_id":4,"titulo":"Drought stress tolerance of Prosopis chilensis and Prosopis flexuosa species and their hybrids","resumen":"Natural hybridization commonly produces individuals with intermediate morphological and genetic characteristics, but their response to environmental stress is still uncertain, with some studies showing that transgressive performance would be common. Prosopis chilensis and Prosopis flexuosa are the most important tree species from Arid Chaco, South-America. Both species occupy different ecological niches in terms of water availability. Genetic and morphological studies have demonstrated the existence of interspecific hybrids in contact areas between these species. Hybrids are characterized by clear intermediate morphological characteristics, which have taxonomical value, and genetic structure compared to both parental species. We studied mechanisms implicated in drought stress tolerance in seedlings of P. chilensis, P. flexuosa and their interspecific hybrids trying to elucidate if hybrids have a morpho-physiological, growth and survival intermediate response to drought compared to differential parental responses or if they out-perform both parental species when subjected to drought. Our results suggest that hybridization does not result in individuals with intermediate mechanisms related to drought resistance, but with a unique trait combination leading to high growth when water availability is high (similar to the most vulnerable parental species) and high survival under drought stress (similar to the more resistant parental species). Certain uncoupling between symplastic and apoplastic resistence to drought was observed in hybrids, as well as decreased physiological-wood anatomical plasticity compared to parental species. The long-term consequences in terms of adaptive response to drought of this particular trait combination of hybrids remain still unknown.","palabras_clave":"HYBRIDIZATION|DROUGHT STRESS|PHENOTYPIC PLASTICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62629,"idioma_id":4,"titulo":"Subresultants in multiple roots","resumen":"We extend our previous work on Poisson-like formulas for subresultants in roots&nbsp; to the case of polynomials with multiple roots in both the univariate and multivariate case, and also explore someclosed formulas in roots for&nbsp; univariate polynomials in this multiple roots setting.","palabras_clave":"Subresultants|Gemeralized Vandermonde determinants"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62630,"idioma_id":4,"titulo":"Yield components, light interception and radiation use efficiency of lucerne (Medicago sativa L.) in response to row spacing","resumen":"Lucerne sowing is characterized by a rectangular spatial arrangement, i.e. greater distance between sowing rows than between plants in the row. Therefore, the reduction of row spacing generates more squarespatial arrangements that can influence intraspecific competition and resource utilization, especially radiation,and thus biomass. The experiment included different row spacings (10¨C15¨C17.5¨C20 and 30 cm) atthe same plant density in a pure lucerne crop during the first production year. Total aerial dry matter(ADM) was increased by narrow spacing (more square arrangements) up to an optimal distance (around13 cm) (R2 = 0.60). ADM ranged from 2292 to 1670 g DM m&#8722;2 for 15 and 30 cm row spacing, respectively. Plant density was the forage yield component most affected by row spacing and it increased with narrowspacing (0.15 ¡Ý R2 ¡Ü 0.5). ADM responses to reduced row spacing were positive as revealed by both a linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.Plant density was the forage yield component most affected by row spacing and it increased with narrowspacing (0.15 ¡Ý R2 ¡Ü 0.5). ADM responses to reduced row spacing were positive as revealed by both a linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.rows than between plants in the row. Therefore, the reduction of row spacing generates more squarespatial arrangements that can influence intraspecific competition and resource utilization, especially radiation,and thus biomass. The experiment included different row spacings (10¨C15¨C17.5¨C20 and 30 cm) atthe same plant density in a pure lucerne crop during the first production year. Total aerial dry matter(ADM) was increased by narrow spacing (more square arrangements) up to an optimal distance (around13 cm) (R2 = 0.60). ADM ranged from 2292 to 1670 g DM m&#8722;2 for 15 and 30 cm row spacing, respectively. Plant density was the forage yield component most affected by row spacing and it increased with narrowspacing (0.15 ¡Ý R2 ¡Ü 0.5). ADM responses to reduced row spacing were positive as revealed by both a linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.Plant density was the forage yield component most affected by row spacing and it increased with narrowspacing (0.15 ¡Ý R2 ¡Ü 0.5). ADM responses to reduced row spacing were positive as revealed by both a linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.i.e. greater distance between sowing rows than between plants in the row. Therefore, the reduction of row spacing generates more squarespatial arrangements that can influence intraspecific competition and resource utilization, especially radiation,and thus biomass. The experiment included different row spacings (10¨C15¨C17.5¨C20 and 30 cm) atthe same plant density in a pure lucerne crop during the first production year. Total aerial dry matter(ADM) was increased by narrow spacing (more square arrangements) up to an optimal distance (around13 cm) (R2 = 0.60). ADM ranged from 2292 to 1670 g DM m&#8722;2 for 15 and 30 cm row spacing, respectively. Plant density was the forage yield component most affected by row spacing and it increased with narrowspacing (0.15 ¡Ý R2 ¡Ü 0.5). ADM responses to reduced row spacing were positive as revealed by both a linear increase in radiation interception (PARia) (R2 = 0.76) and an optimal pattern in radiation use efficiency (RUE) (R2 = 0.45). PARia in the first year showed an increase of 8 MJ cm&#8722;1 of spacing reduction. RUE values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.values ranged from 2.0 to 1.6 g DM MJ&#8722;1 for 15 and 30 cm row spacing, respectively. The leaf area index (LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.(LAI) was also affected by row spacing. Additionally, seasonal variation was found for the main variablesbut did not interact with row spacing. In conclusion, reducing row spacing to an optimal distance is apractice that allows for more favourable spatial arrangements of a lucerne crop and has a positive impacton forage production.","palabras_clave":"ALFALFA|PLANT DENSITY|RADIATION INTERCEPTION|RADIATION USE EFFICIENCY|ROW DISTANCE|SPATIAL ARRANGEMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62631,"idioma_id":4,"titulo":"Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod","resumen":"Chlorpyrifos is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. In recent years, there has been an important decrease in the number of organisms of Planorbarius corneus. Since the presence of pesticides in the water can be one of the reasons for this decrease, it is very important to study the effect of subchronic exposure to environmental concentrations of pesticides on these organisms. The aim of the present work was to investigate different effects of the subchronic exposure to low concentrations of the organophosphate chlorpyrifos in P. corneus and the possibility to use these as biomarkers. To this end, we have exposed the organisms to 0.4 and 5 &#956;g L-1 of chlorpyrifos for 14 days and recorded the number of egg masses, the number of eggs per mass, the number of eggs without embryo, the time to hatching, the % of hatching and survival. We have also determined the activities of cholinesterases, carboxylesterases and glutathione S transferase in whole organism soft tissue and in the gonads. A 14 days exposure to the 0.4 &#956;g L-1 caused an increase in the number of egg masses without eggs and a decrease in carboxylesterases measured with p-nitrophenyl butyrate. However the exposure to 5 &#956;g L-1 also caused an increase in the time to hatching, a decrease in the % of hatching and survival and also inhibition of cholinesterases and carboxylesterases with p-nitrophenyl acetate and butyrate. In contrast, the glutathione S-transferase has not been modified with the tested concentrations. We concluded that in P. corneus exposed to chlorpyrifos for 14 days, the CES determined with p-nitrophenyl butyrate proved to be the most sensitive biomarker. However, exposure to environmental concentrations showed a decrease in the reproduction ability which could cause a decrease in the number of organisms of this specie.","palabras_clave":"CHLORPYRIFOS|CHOLINESTERASES|CARBOXYLESTERASES|GLUTATHIONE S-TRANSFERASE|REPRODUCTION|PLANORBARIUS CORNEUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62632,"idioma_id":4,"titulo":"Dry weight partitioning and hydraulic traits in young Pinus taeda trees fertilized with nitrogen and phosphorus in a subtropical area","resumen":"Plants of Pinus taeda L. from each of four families were fertilized with nitrogen (N), phosphorus (P) or N + P at planting. The H family had the highest growth in dry mass while the L family had the lowest growth. Measurements of plant hydraulic architecture traits were performed during the first year after planting. Stomatal conductance (gs), water potential at predawn (&#936;predawn) and at midday (&#936;midday), branch hydraulic conductivity (ks and kl) and shoot hydraulic conductance (K) were measured. One year after planting, dry weight partitioning of all aboveground organs was performed. Phosphorus fertilization increased growth in all four families, while N fertilization had a negative effect on growth. L family plants were more negatively affected than H family plants. This negative effect was not due to limitations in N or P uptake because plants from all the families and treatments had the same N and P concentration in the needles. Phosphorus fertilization changed some hydraulic parameters, but those changes did not affect growth. However, the negative effect of N can be explained by changes in hydraulic traits. L family plants had a high leaf dry weight per branch, which was increased by N fertilization. This change occurred together with a decrease in shoot conductance. Therefore, the reduction in gs was not enough to avoid the drop in &#936;midday. Consequently, stomatal closure and the deficient water status of the needles resulted in a reduction in growth. In H family plants, the increase in the number of needles per branch due to N fertilization was counteracted by a reduction in gs and also by a reduction in tracheid lumen size and length. Because of these two changes, &#936;midday did not drop and water availability in the needles was adequate for sustained growth. In conclusion, fertilization affects the hydraulic architecture of plants, and different families develop different strategies. Some of the hydraulic changes can explain the negative effect of N fertilization on growth.","palabras_clave":"GENETIC VARIABILITY|HIDRAULIC CONDUCTIVITY|LEAF WATER POTENTIAL|NUTRIENTS|STOMATAL CONDUCTANCE|XYLEM ANATOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62633,"idioma_id":5,"titulo":"Comportamiento de diferentes cultivares de trigo a Wheat streak mosaic virus (WSMV) y High plains virus (HPV) mediante infección artificial con el vector Aceria tosichella Keifer bajo condiciones de campo","resumen":"El trigo (Triticum aestivum L.) es el cereal de invierno de mayor importancia económica en la Argentina. Dado que Wheat Streak Mosaic virus (WSMV) y High Plains virus (HPV) constituyen una de las principales limitantes sanitarias del cultivo de trigo, el objetivo del presente trabajo fue evaluar el comportamiento de diferentes cultivares ante ambas enfermedades en infecciones artificiales, bajo condiciones de campo. El inóculo viral mixto WSMV-HPV y el vector A. tosichella se obtuvieron en campo y se multiplicaron en laboratorio. Los eriófidos infectados con ambos virus se utilizaron para realizar las infecciones en campo. Se evaluó la incidencia, el grado de infección y la severidad de síntomas de las infecciones en los diferentes genotipos de trigo. Los cultivares fueron clasificados según la probabilidad de incidencia de cada virus y su interacción, hallándose diferencias significativas sólo en los casos de infecciones con HPV. No se hallaron diferencias significativas en el comportamiento de los cultivares al WSMV. Sólo el 9,2% de los cultivares estudiados presentó infección de ambas virosis. El grado de severidad observado varió entre leve a moderado para las plantas que resultaron infectadas. Los resultados reportados en el presente trabajo constituyen una herramienta potencial para la selección de genotipos con mejor comportamiento en este contexto productivo. - See more at: http:\/\/ria.inta.gov.ar\/?p=3566#sthash.ZduzTKhm.dpuf","palabras_clave":"Triticum aestivum|mixed viral infection|Eriophyidae|incidence|severity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62634,"idioma_id":5,"titulo":"EFECTO DE LA COMBINACIÓN DE TÉCNICAS DE ESTABLECIMIENTO SOBRE EL CRECIMIENTO INICIAL DE Grevillea robusta A. Cunn.","resumen":"Se evaluó el efecto de la combinación de técnicas de establecimiento sobre el crecimiento inicial de Grevillea robusta, en el NO de la provincia de Corrientes, Argentina (27º36\u201918\u2019\u2019 S y 55º57\u201915\u2019\u2019 O). Los tratamientos incluyeron la combinación de dos factores, el primero de ellos aplicado previo a la plantación de la G. robusta y consistió en el manejo de residuos, con dos niveles: a) conservación de residuos y, b) quema de residuos; El segundo factor consistió en aplicación de fertilizante, con tres niveles: a) sin aplicación de fertilizante, b) aplicación de fertilizante luego del establecimiento de la plantación y, c) aplicación de fertilizante luego de un año de establecida la plantación. La combinación de técnicas de establecimiento de la plantación modificó el crecimiento inicial de G. robusta. La mejor combinación fue la quema de residuos con fertilización durante el establecimiento, con la cual la plantación logró mantener un incremento en diámetro 43.7% superior con respecto al crecimiento en el tratamiento de conservación de residuos sin aplicación de fertilizante, hasta los 36 meses de edad. ","palabras_clave":"SILVICULTURA|MANEJO FORESTAL|FERTILIZACIÓN|RESIDUOS FORESTALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62635,"idioma_id":4,"titulo":"In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum","resumen":"This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of thefungi associated with the grapevine diseases known as ?hoja de malvón? and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: &#945;-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of &#945;-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and &#945;-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis.","palabras_clave":"VITIS|NEROLIDOL|Phaeoacremonium parasiticum"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62636,"idioma_id":4,"titulo":"Study of the effects of spray-drying on the functionality of  probiotic lactobacilli.","resumen":"Lactobacillus casei Nad, L. acidophilus A9 and L. paracasei A13 were spray-dried in 20% (w\/v) skim milk and submitted to a simulated gastrointestinal digestion. IgA-producing cells were enumerated in the small intestine lamina propria after lactobacilli administration to mice. Spray drying significantly enhanced the resistance of L. paracasei A13 and L. casei Nad to a simulated gastrointestinal digestion. Also, a significant higher number of IgA-producing cells was induced by spray-dried cultures compared to fresh cultures. Spray drying is a suitable technological process for the development of probiotic cultures in skim milk with increased functionality for certain strains.","palabras_clave":"spray drying|probiotics|viability|IgA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62637,"idioma_id":5,"titulo":"Epidemiología del Síndrome urémico hemolítico en dos regiones de la provincia de Buenos Aires","resumen":"Los objetivos de este estudio fueron describir y comparar la frecuencia del síndrome urémico he- molítico (SUH), registrada durante el periodo 2005-2010 en pobladores rurales y urbanos de la región centro-sur de la provincia de Buenos Aires y caracterizar la distribución de factores hipotéticos asocia- dos al SUH en ambas poblaciones. Se caracterizaron 82 casos de SUH clínica y epidemiológicamente. Para el análisis estadístico de los datos se utilizó el test de Chi2 o test exacto de Fisher cuando fue necesario, y el test de la mediana. La incidencia específica fue 12.7 casos cada 100 000 habitantes rurales (IC 0-23.5) y 7.1 casos cada 100 000 habitantes urbanos (IC 0-9.5). La mediana de la edad en el total de los pacientes estudiados fue de 27 meses (5-139 meses), siendo significativamente más baja en los niños del área rural. Estos resultados podrían explicarse por el contacto más frecuente con materia fecal de bovinos, el mayor consumo de leche sin pasteurizar y la mayor proporción de familiares que realizan actividades de riesgo, encontrados en la subpobla- ción rural. Aunque el SUH es una enfermedad que se asocia frecuentemente con el consumo de carne picada, la mayor parte de los niños que enfermaron no presentaron este antecedente. Las manifestaciones clínicas fueron semejantes en ambas subpoblaciones. Un tercio de los pacientes urbanos había recibido tratamiento con antibióticos previo al desarrollo del SUH.","palabras_clave":"SUH|Clínica|Epidemiología|Población rural"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62638,"idioma_id":4,"titulo":"Lipid-polymer membranes as carriers for L-tryptophan: molecular and metabolic properties","resumen":"Polymerized liposomes encapsulating L-tryptophan were studied with the aim to characterize them as drug delivery systems for the treatment of several metabolic diseases that need an increased systemic L-tryptophan concentration. polymerized liposomes were obtained by UV irradiation of vesicles containing 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in a 1:1 molar ratio, in the presence of 10 and 50 mol% of L-tryptophan (respect to total lipid concentration). Polymerization efficiency was studied spectrophotometrically. Also, bilayer packing at the polar head region was followed with the Merocyanine 540 (MC540) and specific interactions in the lipopolymers were studied by FTIR. High L-tryptophan concentrations (50 mol% respect to total lipid concentration) induced a higher amount of six- and nine-unit polymers. This phenomenon was induced because the L-tryptophan located outside the lipid membrane was included in it during the polymerization process and was thus responsible for the better accommodate of the polar head region. This was not possible with the lower amount of L-tryptophan (10 mol%). The stability of lipopolymers with different amounts of L-tryptophan was studied through release profiles. Polymerized liposomes with 50 mol% of L-tryptophan were able to retain around 80% of the amino acid after 24 hours, whereas those with 10 mol % of the amino acid were able to retain 20%. The metabolic activity of the Caco-2 cell line was also studied. Cytotoxic effects were low in the presence of polymerized liposomes, rendering a maximum percentage of cell death of 30%. In summary, this work stresses the relevance of nonspecific drug-polymerized membrane binding on L-tryptophan pharmacological interaction with possible pharmaceutical applications in liposomal drug delivery. Moreover, the absence of significant cytotoxic effects allows the system proposed to be applied in human health.","palabras_clave":"Lipopolymers|L-Tryptophan|Drug Delivery"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62639,"idioma_id":5,"titulo":"'Escuela y Universidad. Tensiones, conflictos y debates en torno a espacios compartidos'","resumen":"      Este trabajo describe, y reflexiona sobre,  la experiencia en didáctica de las ciencias naturales realizada con las  docentes de una escuela primaria de Argentina y un equipo de extensión de la Universidad Nacional  de Quilmes (UNQ). Se describe el origen y desarrollo del primer año del  proyecto, contando para ello con el aporte de las voces de las involucradas y  del equipo de trabajo. El abordaje de la tarea consistió en jornadas de  reflexión y asesoramiento para las Ferias y  Exposiciones de Ciencias, resultando de estas actividades ciertos aspectos  relevados que nos parecen concluyentes a la hora de planificar acciones de  extensión universitaria, tales como modos de actuación docente, formación  profesional, y la incidencia de los currículos de formación.      &nbsp;        ","palabras_clave":"Didáctica de las ciencias|Formación docente|Desempeño docente|Ferias de ciencia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62640,"idioma_id":4,"titulo":"Ultrasound-assisted Synthesis of Benzophenones by Stille Cross-coupling Reactions. Optimization via Experimental Design","resumen":"A series of diaryl ketones have been synthesized in good to excellent yields through the selective cross-coupling reaction of benzoyl chlorides with arylstannanes using a sonochemical variation of the Stille coupling. Ultrasound significantly enhances this useful organometallic transformation affording the desired products in higher yields and shorter reaction times than conventional reactions. The scope of the protocol has been explored with a selection of arylstannanes and different acyl chlorides as reaction partners. Remarkably, no by-products resulting from homo-coupling could be detected. The ultrasound-promoted cross-coupling reaction was optimized through experimental design.","palabras_clave":"ULTRASOUND|STILLE CROSS-COUPLING|AROMATIC KETONES|ARYLSTANNANES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62641,"idioma_id":5,"titulo":"El tiempo en las decisiones de los estudiantes de la Universidad Virtual de Quilmes","resumen":"'El presente documento se encuadra en el Proyecto de Investigación \u201CLa relación entre las tutorías virtuales y las trayectorias académicas de los estudiantes en el Programa UVQ\u201D.A partir de los datos surgidos de diferentes fuentes, se aborda el análisis particular de la dimensión tiempo como elemento decisorio tanto para la elección como para la deserción de los estudios en la modalidad virtual. Se plantea a partir de la detección de diferentes percepciones sobre el tiempo la necesidad de diseñar estrategias de intervención tutorial que consideren esta dimensión.'","palabras_clave":"estudiante|virtual|tiempo|decisiones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62642,"idioma_id":4,"titulo":"Textural and thermal properties of low-lipid meat emulsions formulated with fish oil and different binders","resumen":"The effect of the addition of several binders (milk proteins concentrate, whey protein concentrate, thermally treated whey protein concentrate, ovalbumin, hydroxypropylmethylcellulose, methylcellulose, mixtures of &#954;:&#953; carrageenans or xanthan-locust bean gums) to low-lipid meat emulsions formulated with fish oil were compared to control formulations with fat or fish oil without any binder added. Process yields were higher than 96 g\/100 g, except for formulation with hydroxypropylmethylcellulose. Formulations with hydrocolloids presented less weight lost by centrifugation than both controls. The differences in the amount of non-frozen water explain the results obtained for process yield and liquid released by centrifugation. Significant differences in hardness were found between formulations. Addition of milk proteins concentrate, xanthan-locust bean gums, and mix of carrageenans gave the highest hardness, similar to control formulation with fat. Hardness presented a good correlation with the plateau modulus obtained from frequency sweep curves. The mechanical spectra of cooked batters showed a gel-like behavior. Viscoelastic behavior of the cooked batters was satisfactory modeled using broadened BSW equation to predict the mechanical relaxation spectrum in the linear viscoelastic range. Thermo-rheological curves were related with modulated differential scanning calorimetry results.","palabras_clave":"meat product|low fat|texture|binders"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62643,"idioma_id":4,"titulo":"Bioanalytical separation and preconcentration using ionic liquids","resumen":"Ionic liquids (ILs) are non-molecular solvents, which display a number of unique properties such as negligible vapor pressure, thermal stability even at high temperatures, favorable viscosity and miscibility with water and organic solvents. These properties make them attractive alternatives to replace environmentally-unfriendly solvents producing volatile organic compounds. In this article, a critical review of state-of-the-art developments using ILs for separation and preconcentration of bioanalytes in biological samples is presented. Special attention is paid to determination of several organic and inorganic analytes, including not only contaminants (e.g. pesticides, nicotine, opioids, gold, arsenic, lead, etc.) but also functional biomolecules (e.g. testosterone, vitamin B12, hemoglobin) in urine, blood, saliva, hair, and nail samples. A brief introduction of modern microextraction techniques based on ILs such us dispersive liquid-liquid microextraction (DLLME) and single drop microextraction (SDME) is provided. A comparison of IL-based methods in terms of the limit of detection and environmental compatibility is also discussed. Finally, critical issues and challenges that have arisen from the use of ILs in separation and preconcentration techniques are also discussed.","palabras_clave":"Bioanalytical|Ionic Liquid|Separation|Microextraction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62644,"idioma_id":4,"titulo":"Archaeosomes display immunoadjuvant potential for a vaccine against Chagas disease","resumen":"Archaeosomes (ARC), vesicles made from lipids extracted from Archaea, display strong adjuvant properties. In this study, we evaluated the ability of the highly stable ARC formulated from total polar lipids of a new Halorubrum tebenquichense strain found in Argentinean Patagonia, to act as adjuvant for soluble parasite antigens in developing prophylactic vaccine against the intracellular protozoan T. cruzi, the etiologic agent of Chagas disease. We demonstrated for the first time that C3H\/HeN mice subcutaneously immunized with trypanosomal antigens entrapped in these ARC (ARC-TcAg) rapidly developed higher levels of circulating T. cruzi antibodies than those measured in the sera from animals receiving the antigen alone. Enhanced humoral responses elicited by ARC-TcAg presented a dominant IgG2a antibody isotype, usually associated with Th1-type immunity and resistance against T. cruzi. More importantly, ARC-TcAg-vaccinated mice displayed reduced parasitemia during early infection and were protected against an otherwise lethal challenge with the virulent Tulahuén strain of the parasite. Our findings suggest that, as an adjuvant, H. tebenquichense-derived ARC may hold great potential to develop a safe and helpful vaccine against this relevant human pathogen. &nbsp;","palabras_clave":"ARCHAEOSOMES|ADJUVANT|CHAGAS DISEASE|EXPERIMENTAL VACCINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62645,"idioma_id":4,"titulo":"The intervention of Nanotechnology against epithelial fungal diseases","resumen":"Fungal infections can attack epithelial tissues and, according to the immunological state of the  patient, some of them invade deeper organs, becoming seriously life compromising. Besides,  bloodstream and local infections associated with intravascular devices constitute a significant problem  associated with increased mortality. Topical therapy is desirable since, in addition to targeting the site  of infection, it reduces the risk of systemic side effects and increases patient compliance. In this  review we describe the pros and cons of using nano-objects that being toxic in nature could be used to  cover surfaces of medical devices, or can act as carriers for targeted delivery of antifungals to skin.  There were also included nano-objects to improve the ocular delivery of antifungals, classically  suffering from ineffective topical administration, difficult access for systemic medication or local  invasive administration. The new preclinical developments of nanoparticulate agents against  cutaneous and ocular mycosis are grouped in three main sections: 1) In vitro antifungal activity of  metallic nanoparticles, 2) In vitro and in vivo antifungal activity of non metallic nanoparticles 3) Ocular  delivery of non metallic nanoparticles.","palabras_clave":"NANO-OBJECTS|METALLIC NANOPARTICLES|EPHITELIA|FUNGI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62646,"idioma_id":4,"titulo":"Rapid divergent evolution of male genitalia among populations of Drosophila buzzatii","resumen":"Increasing evidence from multiple animal systems suggests that genital evolution and diversification are driven by rapid and strong evolutionary forces. Particularly, the morphology of male genital structures is considered to be among the fastest evolving traits in animal groups with internal fertilization. In this study, we investigated patterns of male genital variation within and between natural populations of the cactophilic fly Drosophila buzzatii in its original geographic distribution range in the Neotropics. We detected significant morphological differences among populations and distinguished five differentiated groups. Moreover, among population differentiation in genital morphology was associated with the degree of geographic isolation among populations and clearly contrasted with the general homogeneity detected for the putatively neutral mitochondrial gene COI. Integrating our present data with previous molecular population genetic surveys, our results suggest that male genital morphology has rapidly diverged after the recent demographic expansion that D. buzzatii has undergone in the arid zones of South America. Because the ?lock and key? hypothesis failed to explain the present pattern, we explored alternative explanations for the observed pattern of genital diversification including drift-facilitated sexual selection.","palabras_clave":"Aedeagus|Chromosomal inversion|PST|Morphological evolution|Drift|COI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62647,"idioma_id":4,"titulo":"An alkaloid fraction extracted from the cactus Trichocereus terscheckii affects fitness in the cactophilic fly Drosophila buzzatii (Diptera, Drosophilidae)","resumen":"The host-plant environment of phytophagous insects directly affects various aspects of an insect\u2019s life cycle. Interestingly, relatively few insect groups have specialized in the exploitation of plants in the Cactaceae family, potentially because of the chemical and ecological challenges imposed by these plants. The cactophilic Drosophila buzzatii Patterson &amp; Wheeler, 1942 is a well-studied model in evolutionary ecology, partially because of its ability to exploit toxic cactus hosts. Previous studies have shown a negative effect on performance when flies are reared in an alternative columnar cactus host of the genus Echinopsis, relative to its primary cactus host, Opuntia. These observations were attributed to the presence of alkaloids in Echinopsis tissues, a chemical deterrent to herbivores that indirectly affects Drosophila larvae; however, the putative toxic effect of alkaloids has never been tested directly in D. buzzatii. The present study is the first attempt to relate chemical extracts in Echinopsis terscheckii Pfeiffer with detrimental effects on D. buzzatii. We assessed the effects of a crude alkaloid extract, rich in phenylethylamines, and a \u2018non-alkaloid fraction\u2019 on viability and adult wing morphology. Our results indicate that rearing larvae on an artificial diet containing different concentrations of the crude alkaloid extract decreased pupalviability and adult size in a concentration-dependent manner. We discuss the role of cactus alkaloids in the evolution of host-plant use in cactophilic flies.","palabras_clave":"Alkaloids|Cactus|Host shifts|Viability|Wing morphology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62648,"idioma_id":4,"titulo":"Reworked pyroclastic beds in the early Miocene of Patagonia: Reaction in response to high sediment supply during explosive volcanic events","resumen":"Two meter-scale pyroclastic levels are interbedded within the early Miocene succession of the Estancia 25 de Mayo (Patagoniense transgression) and Santa Cruz formations in the foreland Austral (or Magallanes) Basin, Argentina. The Lower Pyroclastic Level (LPL) is a tabular body interbedded within offshore marine deposits, laterally continuous for 30 km and varying in thickness from few centimeters to around 4 m. Grain-size grades from coarse to extremely fine ash with upward-fining along with a northeastern-fining trends. Structureless fine to very fine tuffs dominate and rare parallel laminations are the only tractive sedimentary structures. The Upper Pyroclastic Level (UPL) lieswithin lowenergy fluvial deposits and is laterally discontinuous, and it is composedby lenticular bodies reaching a maximum of 15 m thick and 100 m wide, with a concave-up base and a plane top. Grain-size range is similar to the LPL but it coarsens upward. The lower portion of the UPL shows parallel lamination, current ripple lamination and mud drapes with large pumice lapilli and plant debris, whereas the upper portion shows parallel lamination and trough cross-stratification. Both pyroclastic levels are composed mainly of pumice grains and glass shards with minor proportions of quartz and plagioclase crystals and lithic fragments. The LPL shows no mixing with epiclastic material whereas the UPL shows an upward increase in epiclastic material, and an upward increment in the scale of cross-bedding. The large thickness in relation to the possible emission center and the content of plant debris of the LPL does not suggest a direct, submarine, ash-fallout origin. The LPL is interpreted as a deposit of hyperpycnal-flows generated at the coastal zone when tephra-laden rivers plunged into the ocean. Large amounts of well preservedplant debris support the hypothesis of a terrestrial source of the sediments. The UPL is entirely composed of tractive deposits, so an ash fallout origin is disregarded. This, together with the lenticular shape and the alluvial plain origin of the encasing sediments, suggests accumulation within fluvial channels. Cycles of upper-flow-regime parallel lamination, current-ripple lamination and mud drapes at the lower portion, suggest short-lived turbulent flows that initially filled semi-abandoned channels. They were followed by sheet floods and channel reactivation, expressed by large-scale cross-bedding. The low degree of particle mixing observed in both levels is explained by the inability of streams to erode the substrate as they are suddenly over-saturated with pyroclastic sediments during and after the eruption. The grain-size distribution of the LPL and geochemical data indicate a contemporaneous volcanic source located to the west\/southwest in the Andean ranges, where the South Patagonian Batholith is presently located.","palabras_clave":"Pyroclastic deposits|Explosive volcanism|Hyperpycnal flows|Fluvial deposits"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62649,"idioma_id":4,"titulo":"Optimization of rheological properties of gluten-free pasta dough using mixture design","resumen":"The rising demand of gluten-free products for celiac people has led to important technological researchon the replacement of the gluten matrix in the production of high quality gluten-free foods. The objectiveof this work was to evaluate the effect of composition (hydrocolloids, water, and proteins) on therheological and textural properties of gluten-free dough used for producing pasta based on corn-starchand corn flour. Extensibility and rheological properties of gluten-free pasta dough were studied. Risingprotein or gum contents produced a marked increase of deformation at break. However protein contentwas negatively correlated with breaking force. The increase in gums content produced an increase instorage and loss moduli (G0, G00). G0 was always larger than G00 with a small increase of both moduli withfrequency. The mechanical relaxation spectrum was predicted from dynamic oscillatory data using thebroadened BaumgaerteleSchausbergereWinter model. Application of a mixture design allowed findingthe optimal composition to achieve the desirable textural properties using response surface methodology.A formulation containing 35.5% water, 2.5% gums, 4.7% proteins, 42.8% corn-starch, 10.7% corn flour,1% NaCl, and 2.8% sunflower oil led to the highest values of G0 , breaking force, and extensibility accordingto the optimization analysis performed.","palabras_clave":"GLUTEN FREE PASTA|RHEOLOGY|OPTIMIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62650,"idioma_id":4,"titulo":"Rheological analysis of emulsion-filled gels based on high acyl gellan gum","resumen":"Emulsion-filled gels are widely used in cosmetic, food, and pharmaceutical industry. As rheological properties of these systems are strongly dependent on the properties of the gelled polymer network, rheological characteristics of gels containing high and low acyl gellan gum were analyzed. Under the processing conditions low acyl emulsions were unstable, thus in the present work the influence of oil and hydrocolloid concentrations on the viscoelastic behavior of emulsion-filled gels containing high acyl gellan gum was studied. Increasing gellan concentration (from 0.1 g\/100 g to 0.5 g\/100 g) produced stronger gels, while oil fraction (10 g\/100 g - 30 g\/100 g) slightly affected the elastic behavior of the emulsions reinforcing the structure and the elastic characteristics of the gellan matrix. Sauter diameter (d32) was measured for all emulsions and an average value of 12 micrometers was obtained. Rheological data (oscillatory and creeperecovery tests) were successfully modeled to interpret the structural characteristics of the gelled emulsions. The broadened BaumgaerteleSchausbergereWinter spectrum was used to represent the linear viscoelastic behavior of the continuous phase and the emulsified system, showing that the rheological behavior of the systems was controlled by the highly structured continuous phase rather than the contribution of filler lipid droplet in the emulsion. Relaxation spectra were validated using creeperecovery experiments. Regardless of hydrocolloid concentration, creep compliance of the gel emulsions decreased compared with their respective gels, showing that the inclusion of oil droplets produced a reinforcement of the structure and the gel strength of the matrix.","palabras_clave":"GEL EMULSIONS|GELLAN GUM|RHEOLOGY|RELAXATION TIME SPECTRUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62651,"idioma_id":4,"titulo":"Postcranial skeleton of Cricosaurus araucanensis (Crocodyliformes: Thalattosuchia): morphology and palaeobiological insights","resumen":"The metriorhynchid crocodyliform Cricosaurus araucanensis (Gasparini &amp; Dellapé) has been documented from Tithonian (Upper Jurassic) strata ofthe Vaca Muerta Formation exposed in the Neuquén Basin, northwest Patagonia, Argentina. Postcranial components of this species were mentionedbut not described in the original analysis. Subsequently, other authors described the forelimbs. The postcranial elements of metriorhynchids arepoorly documented in comparison with their skulls, but new data from C. araucanensis reveal delayed ossification of the caudal neurocentralsutures indicating skeletal paedomorphosis affecting not only the appendicular skeleton but also the posterior region of the vertebral column. Themorphology of the caudal region (transverse processes of the first caudal vertebrae ventrally deflected) and the reduction in the femur of the fourthtrochanter suggest a reduction of the hypaxial musculature allowing increased epaxial musculature. This pattern of musculoskeletal arrangement isconsistent with the swimming style and propulsion by lateral undulation of the tail, as proposed by previous authors.Cricosaurus araucanensis (Gasparini &amp; Dellapé) has been documented from Tithonian (Upper Jurassic) strata ofthe Vaca Muerta Formation exposed in the Neuquén Basin, northwest Patagonia, Argentina. Postcranial components of this species were mentionedbut not described in the original analysis. Subsequently, other authors described the forelimbs. The postcranial elements of metriorhynchids arepoorly documented in comparison with their skulls, but new data from C. araucanensis reveal delayed ossification of the caudal neurocentralsutures indicating skeletal paedomorphosis affecting not only the appendicular skeleton but also the posterior region of the vertebral column. Themorphology of the caudal region (transverse processes of the first caudal vertebrae ventrally deflected) and the reduction in the femur of the fourthtrochanter suggest a reduction of the hypaxial musculature allowing increased epaxial musculature. This pattern of musculoskeletal arrangement isconsistent with the swimming style and propulsion by lateral undulation of the tail, as proposed by previous authors.C. araucanensis reveal delayed ossification of the caudal neurocentralsutures indicating skeletal paedomorphosis affecting not only the appendicular skeleton but also the posterior region of the vertebral column. Themorphology of the caudal region (transverse processes of the first caudal vertebrae ventrally deflected) and the reduction in the femur of the fourthtrochanter suggest a reduction of the hypaxial musculature allowing increased epaxial musculature. This pattern of musculoskeletal arrangement isconsistent with the swimming style and propulsion by lateral undulation of the tail, as proposed by previous authors.first caudal vertebrae ventrally deflected) and the reduction in the femur of the fourthtrochanter suggest a reduction of the hypaxial musculature allowing increased epaxial musculature. This pattern of musculoskeletal arrangement isconsistent with the swimming style and propulsion by lateral undulation of the tail, as proposed by previous authors.","palabras_clave":"Metriorhynchidae|Neurocentral suture|Paedomorphosis|Jurassic|Vaca Muerta Formation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62652,"idioma_id":4,"titulo":"A new patagonian species of Cricosaurus (Crocodyliformes, Thalattosuchia): first evidence of Cricosaurus in Middle?Upper Tithonian lithographic limestone from Gondwana","resumen":"Upper Jurassic (Tithonian) deposits in north-western Patagonia, Argentina, have  yielded rich and taxonomically diverse assemblages of marine reptiles. These assemblages  are also remarkable by their quality of preservation, and they are represented by ichthyosaurs,  plesiosaurs, turtles and crocodyliforms. Despite the abundant crocodyliform record, only two  metriorhynchid taxa have been identified: Cricosaurus araucanensis and Dakosaurus  andiniensis. Here we described a new species of Cricosaurus, which represents the second  species of Cricosaurus in the Tithonian of the Neuquén Basin, and the first metriorhynchid  found in lithographic limestones from Gondwana. Furthermore, this specimen has the most  complete postcranial remains of any metriorhynchid from South America. The new species is  characterized by a short distance between the premaxilla and the nasal, a relatively narrow  interorbital width, 23?25 mandibular teeth, bicarinated teeth with fine apicobasally aligned  ridges, interalveolar spaces between the first seven teeth approximately 1.5 times longer than  the anteroposterior diameter of the respective alveoli. In order to test the assignment of the  new species to Cricosaurus, we carried out two phylogenetic analyses. In both analyses  Cricosaurus lithographicus sp. nov. is nested with other species referred to this genus. This  new species has peculiar enamel ornamentation, characterized by numerous, fine apicobasally  aligned ridges, when compared to other species of the genus.","palabras_clave":"METRIORHYNCHIDAE|UPPER JURASSIC|NEUQUÉN BASIN|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62653,"idioma_id":4,"titulo":"Numerical Analysis of Si and GaAs Solar Cells Exposed to Space Radiation","resumen":"In this paper, we present a study about Si andGaAs space solar cells by means of numerical simulations. Wehave investigated the variations of the short-circuit current, opencircuit voltage and maximum power point, before and after thedevices were irradiated with 10 MeV protons and fluencesbetween 109 and 1013 p+\/cm2. The simulation results allow toobtain the optimum solar cell with specific requirements forspace applications.","palabras_clave":"CELDA SOLAR|SILICIO|GAAS|SIMULACION NUMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62654,"idioma_id":4,"titulo":"Theoretical study of the maximum power point of n-type and p-type crystalline silicon space solar cells","resumen":"The performance of crystalline silicon n+\/p\/p+ and n+\/n\/p+ solar cells under AM0 spectrum, irradiated with 1 MeV electrons at fluences below 1e17 cm&#8722;2 has been analyzed by means of computer simulation. The software used, fully developed by the authors, solves numerically in one dimension under steady-state conditions, the Poisson and continuity equations self-consistently. The influence of constructive characteristics and different levels of hazardous environmental work conditions on the maximum power point of over 150 devices has been investigated. The study has allowed the authors to propose a useful analytical model related to the constructive characteristics of the device such as polarity, base resistivity and total thickness, with the aim of examining the electrical performance of Si space solar cells. Results presented in this paper are important in order to contribute to the design of radiation-hardened devices.","palabras_clave":"SOLAR CELL|RADIACION|MAXIMUN POWER POINT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62655,"idioma_id":4,"titulo":"Adsorption of Cu(II), Zn(II), Cd(II) and Pb(II) by dead Avena fatua biomass and the effect of these metals on their growth","resumen":"The biosorption of copper(II), zinc(II), cadmium(II) and lead(II) from aqueous solutions by dead Avena fatua biomass and the effect of these metals on the growth of this wild oat were investigated. Pseudo-first- and second-order and intra-particle diffusion models were applied to describe the kinetic data and to evaluate the rate constants. The adsorption kinetics of all the metals follows a pseudo-second-order model.    The adsorption capacity was determined, and the Freundlich and Langmuir models were applied. The experimental data obtained for all the metals are best described by the Langmuir model. A. fatua was characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and zeta potential. The results obtained evidence the presence of Zn(II), Cu(II), Cd(II) or Pb(II) on the surface of the weed.    The growth of A. fatua was affected by the presence of all metals. The decrease in the growth rate with increasing metal concentration was more noticeable for zinc.","palabras_clave":"Avena fatua|biosorption|heavy metals|kinetics|growth"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62656,"idioma_id":4,"titulo":"Co-biosorption of Copper and Glyphosate by Ulva lactuca","resumen":"This study investigated the adsorption of glyphosate (PMG) onto the green algae Ulva lactuca mediated by Cu(II).   Results demonstrated that U. lactuca adsorbs PMG when the process is mediated by Cu(II) with molar ratios Cu(II):PMG &#8805; 1.5:1.   U. lactuca was characterized by water adsorption surface area, FTIR, SEM and EDS. The Langmuir and Freundlich models were applied. Results showed that the biosorption process for copper and PMG in the presence of copper were described by the Langmuir model (qmax=0.85±0.09 mmol g-1, KL=0.55±0.14 l mmol-1 and qmax=3.65±0.46 mmol g-1, KL=0.103±0.03 l mmol-1, respectively). Copper adsorption was greater in the presence of PMG than in the absence of the pesticide and the adsorption can only be represented by the Freundlich model (KF= 0.08±0.01, 1\/n= 1.86±0.07).  In all cases studied, the maximum metal uptake (qmax) increased with increasing pH. Surface complexes with a stoichiometry ranging from &#8801;Cu-PMG-Cu to &#8801;Cu-PMG-Cu3 are suggested as reaction products of the process.   Due to the increasing amounts of PMG applied in Argentina, natural reservoirs present considerable amounts of this herbicide. The value of this work resides in using U. lactuca, a marine seaweed commonly found along coastlines all over the world, as a biosorbent for PMG.","palabras_clave":"BIOSORPTION|COPPER    |GLYPHOSATE|ULVA LACTUCA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62657,"idioma_id":4,"titulo":"Novel animal defenses against predation: A snail egg neurotoxin combining lectin and pore-forming chains that resembles plant defense and bacteria attack toxins","resumen":"Although most eggs are intensely predated, the aerial egg clutches from the aquatic snail Pomacea canaliculata have only one reported predator due to unparalleled biochemical defenses. These include two storage-proteins: ovorubin that provides a conspicuous (presumably warning) coloration and has antinutritive and antidigestive properties, and PcPV2 a neurotoxin with lethal effect on rodents.   We sequenced PcPV2 and studied whether it was able to withstand the gastrointestinal environment and reach circulation of a potential predator.  Capacity to resist digestion was assayed using small-angle X-ray scattering (SAXS), fluorescence spectroscopy and simulated gastrointestinal proteolysis. PcPV2 oligomer is antinutritive, withstanding proteinase digestion and displaying structural stability between pH 4.0\u201310.0. cDNA sequencing and protein domain search showed that its two subunits share homology with membrane attack complex\/ perforin (MACPF)-like toxins and tachylectin-like lectins, a previously unknown structure that resembles plant Type-2 ribosome-inactivating proteins and bacterial botulinum toxins. The protomer has therefore a novel AB toxin combination of a MACPF-like chain linked by disulfide bonds to a lectin-like chain, indicating a delivery system for the former. This was further supported by observing PcPV2 binding to glycocalix of enterocytes in vivo and in culture, and by its hemaggutinating, but not hemolytic activity, which suggested an interaction with surface oligosaccharides. PcPV2 is able to get into predator\u2019s body as evidenced in rats and mice by the presence of circulating antibodies in response to sublethal oral doses.  To our knowledge, a lectin-pore-forming toxin has not been reported before, providing the first evidence of a neurotoxic lectin in animals, and a novel function for ancient and widely distributed proteins.   The acquisition of this unique neurotoxic\/antinutritive\/storage protein may confer the eggs a survival advantage, opening new perspectives in the study of the evolution of animal defensive strategies.","palabras_clave":"NEUROTOXIN|CHEMICAL ECOLOGY|PcPV2|ANIMAL DEFENSE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62658,"idioma_id":4,"titulo":"LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina","resumen":"The Epstein Barr virus (EBV) is associated with several lymphoyd and  pithelial malignancies such as Hodgkin and Burkitt lymphoma or nasopharyngeal carcinoma and it is also the etiological agent of infectious mononucleosis (IM). Transcriptional regulation of the viral oncoprotein LMP1, remains yet not fully understood. LMP1 expression can be initiated in an EBNA2 dependent or independent manner from EDL1 or LT-R1 promoters. It has been proposed that sequence variation at EDL1 region could be an important factor concerning LMP1 expression. In order to characterize the natural sequence variation of the EDL1 promoter, and its relationship with neoplasia, 44 pediatric patients, 17 IM and 27 EBV associated lymphoma cases from Argentina, were studied. Phylogenetic analysis showed 4 main clusters, namely B95.8, Raji, Cao and P3HR1. Most isolates, 80.3%, conformed the B95.8 group. Co-infection with more than one viral variant was detected in 5\/17 IM cases, but no co-infections were detected among lymphoma cases. Moreover, co-infected IM cases exhibited differences between the ED-L1 sequences obtained from different anatomical compartments. Mutations confined to transcription factor binding sites such as SP1\/SP3, CRE, AP2, C\/EBP were found in similar proportions in 23 isolates from both benign and malignant samples, rendering the distribution of these mutations not significant among malignant samples.","palabras_clave":"EBV|LMP1|ED-L1 promotor|mononucleosis infecciosa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62659,"idioma_id":4,"titulo":"Presence of Human Papilloma Virus in a Series of Breast Carcinoma from Argentina","resumen":"Background: The etiology and the molecular mechanisms related to breast carcinogenesis remain poorly understood.   Some recent reports have examined the role of Human Papillomavirus (HPV) in this disease.   The purpose of this study was todetermine the prevalence of HPV in breast cancer.  Methods: Sixty one fresh frozen breast cancers samples were analyzed.   Samples were tested for HPV by PCR, and products were automatically sequenced.   Findings were correlated with clinical and pathological characteristics.  Results: The HPV DNA prevalence in the breast cancer samples was 26% (16\/61).   Clinical parameters were not statistically associated with HPV presence (p.0.05 x2 test).   Sequence analysis in a subgroup of cases indicates the prevalence of low risk HPV11, followed by high risk HPV16. We found no HPV transcriptional activity.  Conclusion: The present study demonstrated for the first time in Argentina the presence of HPV in a proportion   of the malignant breast tissues.   This finding suggests that HPV may have a biological significance in breast carcinogenesis.","palabras_clave":"HPV|Carcinoma de mama|bajo riesgo|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62660,"idioma_id":4,"titulo":"Interpretation of the depths of maximum of extensive air showers measured by the Pierre Auger Observatory","resumen":"To interpret the mean depth of cosmic ray air shower maximum and its dispersion, we parametrize those two observables as functions of the first two moments of the ln A distribution. We examine the goodness of this simple method through simulations of test mass distributions. The application of the parameterization to Pierre Auger Observatory data allows one to study the energy dependence of the mean ln A and of its variance under the assumption of selected hadronic interaction models. We discuss possible implications of these dependences in term of interaction models and astrophysical cosmic ray sources.","palabras_clave":"cosmic ray experiments|ultra high cosmic rays"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62661,"idioma_id":4,"titulo":"Differential mercury transfer in the aquatic food web of a double basined lake associated with selenium and habitat","resumen":"  Food web trophodynamics of total mercury (THg) and selenium (Se) were assessed for the double-basined ultraoligotrophic system of Lake Moreno, Patagonia. Each basin has differing proportions of littoral and pelagic habitats, thereby providing an opportunity to assess the importance of habitat (e.g. foodweb structure or benthic MeHg production) in the transfer of Hg and Se to top trophic fish species. Pelagic plankton, analyzed in three size classes (10?53, 53?200, and &gt;200 µm), had very high [THg], exceeding 200 µg g&#8722;1 dry weight (DW) in the smallest, and a low ratio of MeHg to THg (0.1 to 3%). In contrast, [THg] in littoral macroinvertebrates showed lower values (0.3 to 1.8 µg g&#8722;1 DW). Juvenile and small fish species feeding upon plankton had higher [THg] (0.2 to 8 µg g&#8722;1 muscle DW) compared to large piscivore fish species (0.1 to 1.6 ìg g&#8722;1 muscle DW). Selenium concentrations exhibited a much narrower variation range than THg in the food web, varying from 0.5 to 2.7 µg g&#8722;1 DW. Molar Se:Hg ratios exceeded 1 for the majority of organisms in both basins, with most ratios exceeding 10.   Using stable nitrogen isotopes as indicator of trophic level, no significant correlations were found with [THg], [Se] or Se:Hg. The apparent lack of biomagnification trends was attributed to elevated [THg] in plankton in the inorganic form mostly, as well as the possibility of consistent Se supply reducing the biomagnification in the food web of the organic portion of THg.        ","palabras_clave":"Mercury|Selenium|Freshwater food web|Lake Moreno|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62662,"idioma_id":4,"titulo":"Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance","resumen":"Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal  health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A  better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture  to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium  reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray  hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce  considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels,  responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to  constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674;  and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this  susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during  resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the  mechanisms and pathways conferring resistance to this important disease in maize.","palabras_clave":"Maize|Fusarium|Transcriptomic|Plant defense"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62663,"idioma_id":4,"titulo":"Saturated fat-rich diet increases fetal lipids and modulates LPL and leptin receptor expression in rat placentas","resumen":"Metabolic alterations in obese and overweight mothers impact the placenta and the fetus, leading to anomalies in fetal growth and lipid accretion. The primary aim of the study was to examine the effect of a saturated fat-rich diet (FD) on growth, lipid accretion, and lipases, leptin and leptin receptor (ObR) expression in the placenta and fetal liver. We also aimed to find a role for fetal leptin in the modulation of placental and fetal liver lipase and ObR expression. Six-week-old rats were fed with a standard rat chow (control) or a 25% FD for 7 weeks until mating and during pregnancy. Also, in a group of control rats, fetuses were injected with leptin on days 19, 20, and 21 of pregnancy. On day 21, we assessed lipidemia, insulinemia, and leptinemia in mothers and fetuses. In the placenta and fetal liver, lipid concentration was assessed by thin layer chromatography (TLC) and the gene expression of lipoprotein lipase (LPL), endothelial lipase, insulin receptor (Insr), leptin, and ObR by RT-PCR. The FD induced hypertriglyceridemia and hyperleptinemia (P lower than 0.01) in mothers and fetuses, an increase in maternal (P lower than 0.05) and fetal weight (P lower than 0.01), overaccumulation of lipids in fetal liver (P lower than 0.01), and enhanced leptin expression in the placenta and fetal liver ( P lower than 0.05). Placental expression of IR and LPL was increased (P lower than 0.05), and ObR decreased (P lower than 0.05) in the FD group. Fetal administration of leptin induced the placental and fetal liver downregulation of ObR (P lower than 0.05) and upregulation of LPL expression (P lower than 0.05). The FD led to increased fetal lipid levels, which may result from high maternal lipid availability and fetal leptin effects.","palabras_clave":"fat diet|LPL|leptin receptor|placenta"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62664,"idioma_id":4,"titulo":"Argentina and the Pope from the End of the World: Antecedents and Consequences","resumen":"La designación del cardenal Bergoglio como  nuevo pontífice de la Iglesia Católica tuvo una repercusión que excedió toda  expectativa. La prensa internacional, de hecho, no se había fijado en él antes  del conclave; incluso los mejores vaticanistas erraron sus cálculos. Sin  embargo, bastaron tan sólo unos pocos gestos para que el Papa Francisco lograra  conquistar la atención del orbe católico, sacudido y seducido por los gestos  transparentes y cálidos del Papa. Los flashes de las cámaras y los  interrogantes de los analistas se volcaron sobre él. ¿Quién era Jorge Mario  Bergoglio? Comenzó a circular un sinnúmero de datos biográficos acerca del Papa  argentino que, tomados aisladamente, no eran más que un puñado de migajas  dispersas. Leídos entre líneas, sin embargo, revelan que este hombre hizo una  meteórica carrera eclesiástica, con reiterados viajes al extranjero que  seguramente facilitaron su designación como cardenal (príncipe de la Iglesia)  en 2002. ","palabras_clave":"historia reciente|papa Francisco|iglesia católica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62665,"idioma_id":4,"titulo":"Transcriptional regulation of insulin-degrading enzyme modulates mitochondrial amyloid &#946; (A&#946;) peptide catabolism and functionality","resumen":"Studies of post-mortem brains from Alzheimer diseasepatients suggest that oxidative damage induced by mitochondrialamyloid  (mitA) accumulation is associated with mitochondrialdysfunction. However, the regulation of mitAmetabolism is unknown. One of the proteases involved in mitAcatabolism is the long insulin-degrading enzyme (IDE) isoform(IDE-Met1). However, the mechanisms of its expression areunknown, and its presence in brain is uncertain. We detectedIDE-Met1 in brain and showed that its expression is regulated bythe mitochondrial biogenesis pathway (PGC-1\/NRF-1). Astrong positive correlation between PGC-1 or NRF-1 and longIDE isoform transcripts was found in non-demented brains.This correlation was weaker in Alzheimer disease. In vitro inhibitionof IDE increased mitA and impaired mitochondrial respiration.These changes were restored by inhibition of -secretaseor promotion of mitochondrial biogenesis. Our resultssuggest that IDE-Met1 links the mitochondrial biogenesis pathwaywith mitA levels and organelle functionality.","palabras_clave":"mitochondrial metabolism|transcription factors|insulin-degrading enzyme|amyloid beta"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62666,"idioma_id":4,"titulo":"REDOX METABOLISM IN TRYPANOSOMA CRUZI: FUNCTIONAL CHARACTERIZATION OF TRYPAREDOXINS REVISITED","resumen":"Tryparedoxins (TXNs) are multipurpose oxidoreductases from   trypanosomatids that transfer reducing equivalents from trypanothione to   different thiol proteins. In Trypanosoma cruzi, two genes   coding for TXN like proteins have been identified: TXNI, previously   characterized as a cytoplasmic protein, and TXNII, a putative   tail-anchored membrane protein. In this work, we performed a comparative   functional characterization of T. cruzi TXNs. Particularly, we   cloned the gene region coding for the soluble version of TXNII for its   heterologous expression. The truncated recombinant protein (without its   22 C-terminal transmenbrane amino acids) showed TXN activity. It was   also able to transfer reducing equivalents from trypanothione,   glutathione or dihydrolipoamide to different acceptors, including   methionine sulfoxide reductases and peroxiredoxins. Results support the   occurrence and functionality of a second tryparedoxin, which appears as a   new component in redox scenario in T. cruzi.","palabras_clave":"trypanosoma|tryparedoxin|trypanothione"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62667,"idioma_id":4,"titulo":"EVALUATION OF UV AND VISIBLE LIGHT ACTIVITY OF TIO2 CATALYSTS FOR WATER REMEDIATION","resumen":"The photocatalytic activity under UV, visible and UV-visible radiation of two commercial catalysts for water remediation are evaluated and compared in a slurry reactor. Bisphenol A (BPA) was employed as the model pollutant. The activities of the carbon-doped, TiO2 catalyst Kronos vlp 7000 and, as a reference, the undoped catalyst Aeroxide P25 are compared by means of efficiency parameters: the overall photonic efficiency and the quantum efficiency. For the evaluation of the radiation absorbed by the catalyst suspensions, the optical properties of TiO2 Kronos vlp 7000 between 300 and 550 nm were measured and reported, as well as the properties of TiO2 Aeroxide P25 in the range 400-550 nm. Kronos catalyst exhibits absorption in the visible range and was almost three times more efficient than Aeroxide for BPA degradation under this condition. However, under UV radiation, Aeroxide P25 quantum efficiency was about 1.5 times the corresponding value of Kronos. Similar BPA photonic and quantum efficiencies were obtained under UV-visible radiation with both commercial catalysts.","palabras_clave":"EFFICIENCY|TIO2 OPTICAL PROPERTIES|PHOTOCATALYST|RADIATION ABSORPTION|VISIBLE ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62668,"idioma_id":4,"titulo":"Effects of Cultivar and Irrigated Water Quality on Sugarcane Residue Decomposition","resumen":"In sugarcane cropping systems, green-cane harvesting has progressively replaced the traditional burning of standing crop prior to harvest, increasing the role of decomposition as a mechanism to replenish soil nutrients. We examined the impact of cultivar choice and irrigation water quality on decomposition of sugarcane residue. In two independent litterbag experiments, we isolated the effects of changes in plant residue quality and irrigated water quality. Cultivar residue exhibited significant variation of carbon and nitrogen concentrations and carbon to nitrogen ratio. Decomposition rate varied among cultivars, and those with greater carbon-to-nitrogen ratios decomposed faster than cultivars with lower ratios. Soil irrigated with river water showed a lower mineral and organic nitrogen concentration and decomposition rate than those irrigated with industry effluent wastewater. These results provide empirical evidence that both cultivar choice and water used for irrigation have a limited but significant impact on plant residue decomposition.","palabras_clave":"Litterbag experiment| litter chemistry|nutrient cycling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62669,"idioma_id":4,"titulo":"Characterization of a type O foot-and-mouth disease virus re-emerging in the year 2011 in free areas of the Southern Cone of South America and cross-protection studies with the vaccine strain in use in the region","resumen":"Molecular, antigenic and vaccine matching studies, including protective response in vivo,were conducted with a foot-and-mouth disease type O virus isolated during the outbreak in September 2011 in San Pedro, Paraguay, country internationally recognized as free with vaccination in 1997. The phylogenetic tree derived from complete VP1 sequences as well as monoclonal antibody profiling indicated that this isolate was related to viruses responsible for previous emergencies in free areas of the Southern Cone of South America occurring sporadically between the years 2000 and 2006. Marked differences with the vaccine strain O1\/Campos, including the loss of reactivity with neutralizing MAbs, were  recognized. Levels of protective antibodies induced by the vaccine containing the O1\/ Campos strain against the San Pedro virus and the virus responsible for the previous emergency in 2006 in the Southern Cone assessed by in vitro vaccine matching studies pointed to an insufficient protective response 30 days after vaccination (DPV), which was properly attained at 79 DPV or after revaccination. In agreement with the in vitro  assessment, the in vivo challenge in the Protection against Podal Generalization test in cattle indicated appropriate protection for the San Pedro strain at 79 DPV or after revaccination. The complementary conclusions that can be derived from vaccine matching tests designed differently to fit the various objectives intended: prophylaxis, emergency vaccination or incorporation of new field strains into antigen banks, is evaluated. This is the first report of the antigenic and immunogenic characterization of the variants responsible for emergencies in the Southern Cone of South America and the putative impact of the changes on the cross protection conferred by the vaccine strain.","palabras_clave":"Foot-and-mouth disease virus|Vaccine matching|Molecular epidemiology|Monoclonal antibody profiling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62670,"idioma_id":4,"titulo":"Development of a universal CTL-based vaccine for influenza","resumen":"In pursuit of better influenza vaccines, many strategies are being studied worldwide. An attractive alternative is the generation of a broadly cross-reactive vaccine based on the induction of cytotoxic T-lymphocytes (CTL) directed against conserved internal antigens of influenza A virus. The feasibility of this approach using recombinant viral vectors has recently been demonstrated in mice and humans by several research groups. However, similar results might also be achieved through immunization with viral proteins expressed in a prokaryotic system formulated with the appropriate adjuvants and delivery systems. This approach would be much simpler and less expensive. Recent results from several laboratories seem to confirm this is as a valid option to be considered.","palabras_clave":"Influenza vaccines|Cellular immunity|Cytotoxic-T-lymphocytes|Recombinant antigen|Cross-presentation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62671,"idioma_id":4,"titulo":"Evaluation of the immune response elicited by vaccination with viral vectors  encoding FMDV capsid proteins and boosted with inactivated virus","resumen":"The aim of the present study was to assess the effect of introducing a priming step withreplication-defective viral vectors encoding the capsid proteins of FMDV, followed by aboost with killed virus vaccines, using a suitable BALB\/c mice model. Additionally, theimmune response to other combined vector immunization regimens was studied. For thispurpose, we analyzed different prime-boost immunizations with recombinant adenovirus(Ad), herpesvirus amplicons (Hs) and\/or killed virus (KV) vaccines. The highest antibodytiters were found in the group that received two doses of adjuvanted KV (P < 0.002).Antibody titers were higher in those groups receiving a mixed regimen of vectors,compared to immunization with either vector alone (P < 0.0001). Priming with any of theviral vectors induced a shift of the cytokine balance toward a Th1 type immune responseregardless of the delivery system used for boosting. The highest IgG1 titer was induced bytwo doses of adjuvanted KV (P = 0.0002) and the highest IgG2a titer corresponded to thegroup primed with Ad and boosted with KV (P = 0.01). Re-stimulation of all groups of micewith 0.5 mg of inactivated virus five months later resulted in a fast increase of antibodytiters in all the groups tested. After virus stimulation, antibody titers in the groups thatreceived KV alone or Ad prime-KV boost, were indistinguishable (P = 0.800). Protectionfrom challenge was similar (75%) in the groups of animals that received Ad prime-Hs boostor Ad prime-KV boost, or two doses of oil-adjuvanted KV. The data presented in this studysuggest that sequential immunization with viral vectors-based vaccines combined withprotein-based vaccines have the potential to enhance the quality of the immune responseagainst FMDV.","palabras_clave":"FMDV|PRIME BOOST REGIMENS|ADENOVIRUS VECTORS|HERPESVIRUS AMPLICONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62672,"idioma_id":5,"titulo":"Origen y distribución espacial de linajes maternos nativos en el noroeste y centro oeste argentinos","resumen":"El empleo de los polimorfismos del ADN mitocondrialpara caracterizar a las poblaciones humanas hapermitido distinguir patrones geográficos de distribuciónde linajes, detectando posibles rutas de poblamiento. EnArgentina existen regiones clásicamente identificadas comolibres de pueblos originarios, quedando espacios vacíos deinformación en cuanto a la distribución de linajes nativos.Sin embargo, los pueblos de las regiones del centro-oeste ynoroeste no fueron exterminados sino que fueron asimiladosbiológicamente primero, a la sociedad colonial y luego, ala sociedad estatal. Los procesos de mestizaje tuvieron unatendencia sexo asimétrica permitiendo la conservación delos linajes maternos nativos en elevadas proporciones. Eneste trabajo se analizan 1951 muestras obtenidas en centrosde salud de catorce localidades del centro-oeste y noroestede Argentina y se comprueba que el 90% de los haplogruposmitocondriales son propios de América. La agrupaciónde localidades en base a la distribución de frecuencias dehaplogrupos nativos mediante AMOVA indica que las localidadesde Maimará y La Quiaca constituyen una entidadclaramente diferenciada, en coincidencia con los datos arqueológicosy lingüísticos. Las elevadas frecuencias de haplogrupoD en La Rioja y de haplogrupo A en Villa Tulumayapueden representar fenómenos poblacionales prehispánicos.Se postula entonces la utilidad de abordar a la poblaciónactual como vía de acceso al conocimiento del origen delos linajes mitocondriales nativos y su distribución espacial.","palabras_clave":"ADN mitocondrial|nativos americanos|mestizaje"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62673,"idioma_id":4,"titulo":"5-HTTLPR genetic diversity and mode of subsistence in Native Americans","resumen":"The relationship between the \u201Cindividualism-collectivism\u201D and the   serotonin transporter functional polymorphism (5-HTTLPR), suggested in   the previous reports, was tested in Native South Amerindian populations.   A total of 170 individuals from 21 populations were genotyped for the   5-HTTLPR alleles. For comparative purposes, these populations were   classified as individualistic (recent history of hunter\u2013gathering) or   collectivistic (agriculturalists). These two groups showed an almost   identical S allele frequency (75 and 76%, respectively). The analysis of   molecular variance showed no structural differencesbetween them.   Behavioral typologies like those suggestedby JY Chiao and KD   Blizinsky (Proc R Soc B 277 (2010) 529\u2013537) are always a simplification   of complex phenomena and should be regarded with caution. In addition,   classification of a whole nation in the individualist\/ collectivist   dichotomy is controversial. The focus on modes of subsistence in   preindustrial societies, as was tested here, may be a good alternative   although the postulated association between the 5-HTTLPR S allele and   the collectivist societies was not confirmed.","palabras_clave":"5-HTTLPR|gene-culture coevolution|Amerindians|individualism-collectivism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62674,"idioma_id":4,"titulo":"PPAR gamma ligand treatment inhibits cardiac inflammatory mediators induced by infection with different lethality strains of Trypanosoma cruzi.","resumen":"Trypanosoma cruzi (T. cruzi), the etiological agent of Chagas´ disease, causes cardiac alterations in the host. Although the main clinical manifestations arise during the chronic stage, the mechanisms leading to heart damage develop early during infection. In fact, an intense inflammatory response is observed from acute stage of infection. Recently, peroxisome proliferator-activated receptors (PPARs) have attracted research interest due to their participation in the modulation of inflammation. In this work we addressed the role of 15-Deoxy-&#8710;12,14 ProstaglandinJ2 (15dPGJ2), a PPAR&#947; natural ligand in the regulation of inflammatory mediators, in acute and chronic experimental mouse models of Chagas´ disease with the RA and K98 T. cruzi strains, respectively. This work demonstrates that 15dPGJ2 treatment inhibits the expression and activity of inducible nitric oxide synthase (NOS2) as well as TNF-&#945; and IL-6 mRNA levels. Also, expression and activity of metalloproteinases 2 (MMP-2) and 9 (MMP9) were inhibited by 15dPGJ2. Moreover GW9662, a specific PPAR&#947; antagonist, revealed the participation of other signaling pathways since, in GW9662 presence, 15dPJG2 had a partial effect on the inhibition of inflammatory parameters in the acute model of infection. Accordingly, NF-&#954;B activation was demonstrated, assessing p65 nuclear translocation in the hearts of infected mice with both T. cruzi strains. Such effect was inhibited after 15dPGJ2 treatment. Our findings support the concept that in vivo PPAR&#947; and NF-&#954;B pathways are implicated in the inhibitory effects of 15dPGJ2 on inflammatory mediators at different times depending on whether the infection is caused by the lethal or non-lethal T. cruzi strain.","palabras_clave":"PPAR gamma|T cruzi|heart|inflammation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62675,"idioma_id":5,"titulo":"Modelos para Asistir la Gestión de Proyectos de Explotación de Información","resumen":"    Los proyectos de Explotación de Información son un tipoespecial de proyecto de Ingeniería en Software. En lugar de requerirdesarrollar un software específico, herramientas disponibles son utilizadas queya incluyen las técnicas y algoritmos necesarios. Pero de todas formas poseeproblemas similares al existir cuestiones de gestión que todavía deben sermejorados. Entre estas cuestiones se destaca la necesidad de un análisis de viabilidadque permita identificar los riesgos en forma temprana y un método de estimaciónde esfuerzo que asista la planificación de actividades y recursos que sonnecesarios para desarrollar el proyecto. En este contexto, este trabajo tienecomo objetivo proponer y estudiar dos modelos para ser utilizados en Pequeñas yMedianas Empresas al comienzo de un proyecto de Explotación de Información y deesta forma buscar reducir los problemas que se pueden presentar.  ","palabras_clave":"Gestión de Proyectos de Explotación de Información|Modelos de Gestion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62676,"idioma_id":4,"titulo":"Phylogenetic analyses of typical bovine rotavirus genotypes G6, G10, P[5] and P[11] circulating in Argentinean beef and dairy herds","resumen":"Group A rotavirus (RVA) is one of the main causes of neonatal calf diarrhea worldwide. RVA strains affecting Argentinean cattle mainly possess combinations of the G6, G10, P[5] and P[11] genotypes. To determine RVA diversity among Argentinean cattle, representative bovine RVA strains detected in diarrheic calves were selected from a survey conducted during 1997-2009. The survey covered the main livestock regions of the country from dairy and beef herds. Different phylogenetic approaches were used to investigate the genetic evolution of RVA strains belonging to the prevalent genotypes. The nucleotide phylogenetic tree showed that all genotypes studied could be divided into several lineages. Argentinean bovine RVA strains were distributed across multiple lineages and most of them were distinct from the lineage containing the vaccine strains. Only the aminoacid phylogenetic tree of G6 RVA strains maintained the same lineages as observed at the nucleotide level, whereas a different clustering pattern was observed for the aminoacid phylogenetic trees of G10, P[5] and P[11] suggesting that the strains are more closely related at the aminoacid level than G6 strains. Association between P[5] and G6(IV), prevalent in beef herd, and between P[11] and G6(III) or G10 (VI and V), prevalent in dairy herds, were found. In addition, Argentinean G6(III), G10, P[5] and P[11] bovine RVA strains grouped together with human strains, highlighting their potential for zoonotic transmission. Phylogenetic studies of RVA circulating in animals raised for consumption and in close contact with humans, such as cattle, contribute to a better understanding of the epidemiology of the RVA infection and evolution.","palabras_clave":"Bovine|Diarrhea |Phylogeny|Rotavirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62677,"idioma_id":4,"titulo":"Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants","resumen":".","palabras_clave":"BVDV|Vaccine|Transgenic plants"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62678,"idioma_id":4,"titulo":"Effect of organic calcium salts-inulin systems on hydration and thermal properties of wheat flour","resumen":"The aim of this work was to study the effect of calcium acid salts?inulin systems on hydration and thermal properties of wheat flour dough. Wheat flour was enriched with calcium lactate (CaLa2) or calcium citrate (Ca3Ci2) (1080?2520 ppm Ca) and inulin (In) (1%?13%, w\/w flour basis). Water absorption (Wabs), moisture content (Mcont), water activity (aw) and relaxation time (&#955;) of dough were analyzed. Pasting properties during heating?cooling process were studied: peak viscosity (PV), breakdown (BD), final viscosity (FV) and setback 1(SB1) were determined. Temperatures (TpI, TpII) and enthalpy (&#916;Hgel) of gelatinization of dough were analyzed by DSC. Samples with Ca and In presented lower Wabs than control sample with an In2 dependence, with slight difference between both surface responses. More time for dough development (td) was necessary with Ca3Ci2 than with CaLa2, being td independent of calcium content at In level (&#8805;6.5%). Dough with Ca3Ci2 was more stable with less degree of softening than CaLa2-dough, due to the protein stabilizing effect of citrate ion (Hofmeister series) with a maximum at 6.5% In. Mcont and &#955; decreased with the increase of In, independently of calcium. Hydration properties directly influenced pasting parameters. The increase in In content decreased viscosity (PV, FV) without affecting BD. SB1 behavior suggests the formation of pastes with low and high stability with CaLa2 and Ca3Ci2, respectively. Gelatinization degree decreased (40%) and retarded (&#916;T=10 °C) at high levels of both ingredients. CaLa2 had more influence in hydration and thermal properties of wheat flour?inulin blends, enhancing a high degree of inhibition of gelatinization and leading to pastes with low viscosity after cooling. This behavior was influenced by the presence of inulin.","palabras_clave":"Calcium lactate\/citrateinulin system|Wheat flour|Hydration properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62679,"idioma_id":4,"titulo":"Wheat varietal flours: influence of pectin and DATEM on dough and bread quality","resumen":"The effect of two additives, high methoxyl pectin (P) and the emulsifier Diacetyl Tartaric Acid Esters of Monoglycerides commonly named DATEM (D) and their mixture (P+D) on dough properties and baking performance of two varietal argentinean wheat flours (Buck Pronto- BP and Klein Escudo- KE) was analyzed. Rheological characterization of dough (alveogram, farinograms, texture profile analysis-TPA and rheometric assays), with and without additives, was performed. SEM was used to evaluate the microstructure of dough. Baking performance was analysed by bread volume measurements, shape ratio of loaves width\/height) and the hardness of crumb and crust. Assays on dough showed differences in alveographic force (W) and in most of TPA parameters. Assays on bread showed that BP specific volume was improved with the addition of P and P+D but shape ratio was only improved with the mixture of P+D. Breads from KE flour with additives presented, in all cases, higher volumes and a better shape ratio than those obtained with the control sample. Hardness of KE crumb was diminished by all additives but BP crumb was softened only P and P+D. All sensory parameters were improved for both types of bread, particularly with D and P+D.","palabras_clave":"wheat cultivars|wheat dough|food additives|rheological properties|breadmaking quality|sensory evaluation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62680,"idioma_id":4,"titulo":"Kinetics of extracellular ATP in mastoparan 7 activated human erythrocytes","resumen":"The peptide mastoparan 7 (MST7) stimulated ATP release in human  erythrocytes. We explored intra- and extracellular processes governing the time-dependent  accumulation of extracellular ATP (i.e., ATPe kinetics).  Methods: Human erythrocytes were treated with MST7 in the presence or absence of two blockers of  pannexin 1. ATPe concentration was monitored by luciferin-luciferase based real-time luminometry.  Results: Exposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a  slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe  hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes  to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling.  However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe  accumulation was inhibited by 49%.  Erythrocytes pre-exposure to 10 &#956;M of either carbenoxolone or probenecid, two blockers of pannexin  1, exhibited a partial reduction of ATP efflux.  Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice  erythrocytes exposed to pannexin 1 blockers.  Conclusions: MST7 induced release of ATP required either cell adhesion or strong activation of cAMP  synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested  that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being  fully blocked by pannexin 1 blockers.","palabras_clave":"ATPrelease|REDcells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62681,"idioma_id":4,"titulo":"Remating behaviour in Anastrepha fraterculus (Diptera: Tephritidae) females is affected by male juvenile hormone analogue treatment but not by male sterilization","resumen":"The sterile insect technique (SIT) has been proposed as an area-wide method to control the South American fruit fly,Anastrepha fraterculus (Wiedemann). This technique requires sterilization, a procedure that affects, along with other factors, the ability of males to modulate female sexual receptivity after copulation. Numerous pre-release treatments have been proposed to counteract the detrimental effects of irradiation, rearing and handling and increase SIT effectiveness. These include treating newly emerged males with a juvenile hormone mimic (methoprene) or supplying protein to the male´s diet to accelerate sexual maturation prior to release. Here, we examine how male irradiation, methoprene treatment and protein intake affect remating behavior and the amount of sperm stored in inseminated females. In field cage experiments, we found that irradiated laboratory males were equally able to modulate female remating behavior as fertile wild males. However, females mated with 6-day-old, methoprene-treated males remated more and sooner than females mated with naturally matured males, either sterile or wild. Protein intake by males was not sufficient to overcome reduced ability of methoprene-treated males to induce refractory periods in females as lengthy as those induced by wild and naturally matured males. The amount of sperm stored by females was not affected by male irradiation, methoprene treatment or protein intake. This finding revealed that factors in addition to sperm volume intervene in regulating female receptivity after copulation. Implications for SIT are discussed.","palabras_clave":"South American fruit fly|SIT|methoprene|sperm storage"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62682,"idioma_id":4,"titulo":"Burrowing Crab (Neohelice granulata) as a Potential Bioindicator of Heavy Metals in the Bahia Blanca Estuary, Argentina","resumen":"Bioindicators can be used to determine spatialand\/or temporal variations of heavy metals in a certaincoastal or marine environments. This study investigated thepotential use of the burrowing crab Neohelice granulatafrom two different locations in the Bah&#305;´a Blanca estuary, amoderately polluted ecosystem. Concentrations of zinc (Zn),nickel (Ni), and lead (Pb) in soft tissues of male and femalecrabs were measured. In addition, concentrations of the threemetals in eggs were compared with concentrations in femalecrabs. No geographical differences were found for any of thethree metals, whereas sexual and seasonal differences wereobtained for Zn and Ni, with the winter season posing lowerconcentrations. Moreover, the three metals were detectablein eggs and were lower than concentrations in female crabs(except for Zn). Finally, the usefulness of this species as apotential bioindicator of heavy-metal pollution within thisestuarine ecosystem is discussed.","palabras_clave":"Bahia Blanca estuary|heavy metals pollution|bioindicators|ecosystem monitoring"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62683,"idioma_id":4,"titulo":"Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; the influence of PpIX concentration and illumination parameters","resumen":"Light fractionation, with a long dark interval, significantlyincreases the response to ALA-PDT in pre-clinical models and innon-melanoma skin cancer. We investigated if this increase inefficacy can be replicated in PAM 212 cells in vitro. The resultsshow a significant decrease in cell survival after light fractionationwhich is dependent on the PpIX concentration and light dose ofthe first light fraction. This study supports the hypothesis that anunderlying cellular mechanism is involved in the response to lightfractionation in which a first light fraction leads to sub-lethallydamaged cells that are sensitised to a second light fraction2 hours later. The current study reveals the in vitro circumstancesunder which we can investigate the cellular pathways involved.","palabras_clave":"ALA|PHOTODYNAMIC THERAPY|FRACTIONATION|LIGHT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62684,"idioma_id":4,"titulo":"HOST SELECTION, OVIPOSITION BEHAVIOR AND LEAF TRAITS IN A SPECIALIST WILLOW SAWFLY ON SPECIES OF Salix (Salicaceae)'","resumen":"Interacciones influencias genotipo de la planta planta-herbívoro al afectar la atracción de insectos, la aceptación y el desarrollo. Aquí hemos vinculado el comportamiento de oviposición de la mosca de sierra del sauce especialista Nematus oligospilus Förster (Hymenoptera: Tenthredinidae) con rasgos de hojas en diferentes Salix L. (Salicaceae) genotipos. Esto se hizo como un primer paso para encontrar señales de oviposición que guían a las mujeres a sus plantas hospederas.  Por medio de la elección y bioensayos Nohaydeotra analizamos la selección anfitriona en función del genotipo del sauce y la superficie de la hoja. También se estudió el rendimiento larval, la fecundidad adulta y efecto de la experiencia en la selección anfitriona. Nematus oligospilus prefiere poner huevos en S. nigra . El genotipo preferido menos, S. viminalis , demostraron un mayor rendimiento de las larvas y la más alta fecundidad de adultos. Preferencia de acogida no se modificó por la experiencia alimentación de las larvas.  Por medio de microscopía óptica y electrónica de barrido describimos ovipositor y hoja micromorfología. El huevo es puesto dentro de la epidermis o entre la epidermis y clorénquima adyacente, que muestra una estrecha asociación con la hoja. La dureza de la hoja fue menor para S. nigra y superior para S. viminalis y S. babylonica . El contenido total de nitrógeno y proteína fueron mayores en S. viminalis y S. babylonica . Fenoles totales y glucósidos fenólicos fueron las más altas y más diversa en S. nigra . Niveles de contenido de salicina se correlacionan con la preferencia de oviposición, lo que sugiere el papel de salicilatos como estimulantes de oviposición.  Los resultados sugieren que la preferencia de oviposición en S. nigra puede estar relacionado con menor dureza de la hoja y la facilidad de inyección de mujer ovipositor en forma de sierra, y motivado por la presencia de glucósidos fenólicos. Los niveles de nitrógeno pueden explicar mejor rendimiento larval y la fecundidad adulta en S. viminalis . Por lo tanto, un equilibrio entre los diferentes rasgos de la hoja determina los resultados observados en este estudio.","palabras_clave":"willow|plant defense|secondary metabolite|chemical defenses"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62685,"idioma_id":4,"titulo":"Family Specific Degenerate Primer Design: A tool to design consensus degenerated oligonucleotides","resumen":"Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer software, for which the starting point is a multiple alignment of related amino acids or nucleotide sequences. To assess their efficiency, four different genome collections were used, covering a wide range of genomic lengths: Arenavirus (10 × 104nucleotides), Baculovirus (0.9 × 105to 1.8 × 105bp), Lactobacillus sp. (1 × 106to 2 × 106bp), and Pseudomonas sp. (4 × 106to 7 × 106bp). In each case, FAS-DPD designed primers were tested computationally to measure specificity. Designed primers for Arenavirus and Baculovirus were tested experimentally. The method presented here is useful for designing degenerate primers on collections of related protein sequences, allowing detection of new family members.","palabras_clave":"oligonucleotide design|degenerate primer|arenavirus|baculovirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62686,"idioma_id":4,"titulo":"Molecular Dynamics Study on the Encapsulation of Prilocaine in Liposomes at Physiological pH","resumen":"In this work, we investigated the concentration effects on the encapsulation of prilocaine (PLC), an aminoamide local anesthetic, into a small unilamellar liposome, at physiological pH. On the line of our previous work, we have carried out Molecular Dynamics (MD) simulations using a coarse grain (CG) model that allow us to reach the microsecond time scale. At physiological pH there is a partition between protonated and neutral PLCs. In order to estimate the protonated\/neutral PLC ratio at physiological pH (7.4), we have used the Henderson-Hasselbach equation. We have studied three PLC:lipid molar concentrations, ranging between 10:10 to 1:4. We essentially found that all neutral PLC molecules rapidly diffuse into the hydrophobic region of the vesicle adopting an asymmetric bimodal distribution. Moreover, protonated PLC molecules partition between the external monolayer of the vesicle and the water phase, having a high rate of exchange between this two phases, with no access to the inner part of the liposome in a concentration dependent way. In this way, we found that the behavior of PLCs at physiological pH is a combination of high and low pH, especially at low concentration of local anesthetics.","palabras_clave":"LIPOSOMA|ANESTESICOS|LIPIDOS|SIMULACIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62687,"idioma_id":4,"titulo":"SIMILARITIES AND DIFFERENCES OF SEROTONIN AND ITS PRECURSORS IN THEIR INTERACTIONS WITH MODEL MEMBRANES STUDIED  BY MOLECULAR DYNAMICS SIMULATION","resumen":"In this work, we report a Molecular Dynamics (MD) simulations study of relevant biological  molecules as serotonin (neutral and protonated) and its precursors, tryptophan and 5-hydroxytryptophan,  in a fully hydrated bilayer of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine  (POPC). The simulations were carried out at the fluid lamellar phase of POPC at  constant pressure and temperature conditions. Two guest molecules of each type were initially  placed at the water phase. We have analyzed, at the atomic level, the main localization,  preferential orientation and specific interactions of the guest molecules this compounds within  the bilayer. During the simulation run, the four molecules were preferentially found at the waterlipid  interphase. We found that the interactions that stabilized the systems are essentially  hydrogen bonds, salt bridges and cation-&#960;. None of the guest molecules have access to the  hydrophobic region of the bilayer. Besides, zwitterionic molecules have access to the water  phase, while protonated serotonin is anchored in the interphase. Even taking into account  that these simulations were done using a model membrane, our results suggest that the studied  molecules could not cross the blood brain barrier by diffusion. These results are in good  agreement with works that show that serotonin and Trp do not cross the BBB by simple  diffusion - specific carriers are pointed out as the responsible for their transport.","palabras_clave":"DINAMICA MOLECULAR|SEROTONINA|TRIPTOFANO|LIPIDOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62688,"idioma_id":4,"titulo":"CYCLIC-DI-GMP SIGNALING REGULATES MOTILITY AND BIOFILM FORMATION IN BORDETELLA BRONCHISEPTICA","resumen":"The signaling molecule 3´,5´-cyclic diguanylic acid (c-di-GMP) is a  central regulator of diverse cellular functions, including motility, biofilm  formation, cell cycle progression, and virulence in bacteria. Multiple  diguanylate cyclase and phosphodiesterase domain-containing proteins (GGDEF and  EAL\/HD-GYP, respectively) modulate the levels of the second messenger c-di-GMP  to transmit signals and obtain such specific cellular responses. In Bordetella genus this c-di-GMP network  is poorly studied. In this work, we evaluated the expression of two phenotypes  in Bordetella bronchiseptica  regulated by c-di-GMP, biofilm formation and motility, under the ectopic  expression of Pseudomonas aeruginosa proteins with EAL or GGDEF domain  that regulates the c-di GMP level. In agreement with previous reports for other  bacteria, we observed that B. bronchiseptica is able to form biofilm and  reduce its motility only when GGDEF domain protein is expressed. Moreover we  identify a GGDEF domain protein (BB3576) with diguanylate cyclase activity that  participates in motility and biofilm regulation in B. bronchiseptica. These results demonstrate for the first time the  presence of c-di-GMP regulatory signaling in B. bronchiseptica.","palabras_clave":"BORDETELLA|C-DI-GMP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62689,"idioma_id":4,"titulo":"Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli","resumen":"Broccoli is an important component of the human dietwith a high nutritional value. This vegetable is harvested when its developmentis not complete, triggering a quick senescence that is accompanied by chlorophylldegradation and a shortening of shelf life. Recently it was shown that the timeof the day at which the broccoli is harvested can influence in the rate ofchlorophyll degradation. This report describes changes of expression of chlorophyll degrading relatedgenes during postharvest storage of heads harvested at different moments ofday. At harvest, the content of chlorophyll did not change among samples butthe expression of many genes associated with chlorophyll degradation did. Thelevel of chlorophyll diminished during storage, but samples harvested at 18:00h had a lower rate of degradation in comparison with heads harvested at 08:00 h.Most of genes that were previously associated with chlorophyll degradationduring senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed alower expression or a delay in their mRNA level increments in samples harvestedat 18:00 h. Other genes related to chlorophyll degradation during senescence,like BoNYC or BoRCCR, showed an increased expression during senescence but therewas no positive correlation between chlorophyll catabolism and expression ofthese genes. In conclusion, changes in the metabolism due to time of day atharvest not only influence the expression of genes during the day but also maycause different patterns of expression during postharvest. ","palabras_clave":"BROCCOLI|POSTHARVEST|SENESCENCE|DAYTIME|CHLOROPHYLL DEGRADING GENES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62690,"idioma_id":4,"titulo":"Agroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamiana","resumen":"Cotton blue disease is the most important viral disease of cotton in the southern part of South America. Its etiological agent, cotton leafroll dwarf virus (CLRDV), is specifically transmitted to host plants by the aphid vector (Aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. This limitation has held back the study of this virus and the disease it causes. In this study, a full-length cDNA of CLRDV was constructed and expressed in vivo under the control of cauliflower mosaic virus 35S promoter. An agrobacterium-mediated inoculation system for the cloned cDNA construct of CLRDV was developed. Northern and immunoblot analyses showed that after several weeks the replicon of CLRDV delivered by Agrobacterium tumefaciens in Gossypium hirsutum plants gave rise to a systemic infection and typical blue disease symptoms correlated to the presence of viral RNA and P3 capsid protein. We also demonstrated that the virus that accumulated in the agroinfected plants was transmissible by the vector A. gossypii. This result confirms the production of biologically active transmissible virions. In addition, the clone was infectious in Nicotiana benthamiana plants which developed interveinal chlorosis three weeks postinoculation and CLRDV was detected both in the inoculated and systemic leaves. Attempts to agroinfect Arabidopsis thaliana plants were irregularly successful. Although no symptoms were observed, the P3 capsid protein as well as the genomic and subgenomic RNA were irregularly detected in systemic leaves of some agroinfiltrated plants. The inefficient infection rate infers that A. thaliana is a poor host for CLRDV. This is the first report on the construction of a biologically-active infectious full-length clone of a cotton RNA virus showing successful agroinfection of host and non-host plants. The system herein developed will be useful to study CLRDV viral functions and plant-virus interactions using a reverse genetic approach","palabras_clave":"CLRDV|clon infectivo|algodón |polerovirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62691,"idioma_id":4,"titulo":"Synthesis of 6-azaprogesterone and 19-hydroxy-6-azasteroids","resumen":"19-Hydroxy-6-azapregnanes were obtained from pregnenolone via a 7-azido-5-oxo-6-nor-5,7-seco-pregnane intermediate. The 6-azapregnane core was built in good yield in a straightforward way from the secosteroid, by means of a Staudinger (aza-Wittig) reaction. Finally the 19-hydroxy-6-azapregnane was transformed into 19-hydroxy-6-azaprogesterone (that cyclized spontaneously to the 19->3 hemiketal) and 6-azaprogesterone. The 6-azapregnanes lacked agonistic\/antagonistic activity on the progesterone receptor.","palabras_clave":"Azasteroids|6-Azaprogesterone|19-Hydroxypregnane|Gestagenic activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62692,"idioma_id":4,"titulo":"Favored isolation and rapid identication of the astaxanthin-producing yeast Xanthophyllomyces dendrorhous(Phaffia rhodozyma) from environmental samples","resumen":"Xanthophyllomyces dendrorhous (Phaffia rhodozyma) yeasts are biotechnologically exploited as a natural source of astaxanthin for aquaculture. Based on results of recent studies, it has become clear that this species possesses a greater genetic variability generating the necessity to uncover it and assess its potential for the astaxanthin industry. However, difficulties for the isolation of the X. dendrorhous hinder extensive environmental surveys which need to be carried out to better understand the habitat, distribution and genetic diversity of this species. We extensively searched for distinctive physiological traits of X. dendrorhours by testing phenotypic properties simultaneously with a panel of common sympatric fungi. As a result we obtained a new and innovative strategy for improving X. dendrorhous recovery rate and identification from environmental samples. This strategy involved the use of trehalose-based media, and a rapid X. dendrorhous identification method based on the simultaneous spectrophotometric detection of astaxanthin and UV-absorbing compounds (mycosporines). The proposed procedures proved effective in field trials conducted in natural environments of Patagonia (Argentina) and thus represent an important tool for the discovery of new astaxanthin-producing strains of X. dendrorhous useful for the aquaculture industry.","palabras_clave":"Trehalose|Astaxanthin|Mycosporines|Yeasts|Xanthophyllomyces dendrorhous"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62693,"idioma_id":4,"titulo":"Enhancer turnover and conserved regulatory function in vertebrate evolution","resumen":"Mutations in regulatory regions including enhancers are an important source of variation and innovation during evolution. Enhancers can evolve by changes in the sequence, arrangement and repertoire of transcription factor binding sites, but whole enhancers can also be lost or gained in certain lineages in a process of turnover. The proopiomelanocortin gene (Pomc), which encodes a prohormone, is expressed in the pituitary and hypothalamus of all jawed vertebrates. We have previously described that hypothalamic Pomc expression in mammals is controlled by two enhancers?nPE1 and nPE2?that are derived from transposable elements and that presumably replaced the ancestral neuronal Pomc regulatory regions. Here, we show that nPE1 and nPE2, even though they are mammalian novelties with no homologous counterpart in other vertebrates, nevertheless can drive gene expression specifically to POMC neurons in the hypothalamus of larval and adult transgenic zebrafish. This indicates that when neuronal Pomc enhancers originated de novo during early mammalian evolution, the newly created cis- and trans-codes were similar to the ancestral ones. We also identify the neuronal regulatory region of zebrafish pomca and confirm that it is not homologous to the mammalian enhancers. Our work sheds light on the process of gene regulatory evolution by showing how a locus can undergo enhancer turnover and nevertheless maintain the ancestral transcriptional output.","palabras_clave":"transgenic zebrafish|proopiomelanocortin|hypothalamus|enhancers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62694,"idioma_id":4,"titulo":"Estradiol increases dendritic length and spine density in CA1 neurons of the hippocampus of spontaneously hypertensive rats: A Golgi impregnation study","resumen":"Increased neuronal vulnerability has been described in the brain of spontaneously hypertensive rats (SHR), models of primary hypertension. Previous data indicate that estradiol treatment corrects several dysfunctions of the hippocampus and hypothalamus of SHR. Considering this evidence we analyzed the dendritic arborization and spine density of the CA1 subfield in SHR and Wistar-Kyoto (WKY) normotensive rats with and without estradiol treatment. Five month old male SHR and WKY rats received single estradiol or cholesterol pellets (sham treatment) for 2 weeks. A substantial rise of circulating estradiol (>25 fold) and testicular atrophy was present in all estradiol-receiving rats. In both SHR and WKY rats, estradiol decreased blood pressure by ~20 mm Hg; however, a moderate hypertension persisted in SHR (164 mm Hg). Using a modified Golgi impregnation technique, apical and basal dendrites of the CA1 subfield were subjected to Sholl analysis. Spine density was also statistically analyzed. Apical dendritic length was significantly lower in SHR compared to WKY rats (p","palabras_clave":"Hypertension|Hippocampus|Dendritic plasticity|Estradiol"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62695,"idioma_id":4,"titulo":"Cationic lipid:DNA complexes allow bleomycin uptake by melanoma cells","resumen":"Bleomycin is a chemotherapeutic agent barely diffusible through the plasmatic membrane. We evaluated DNA\/cationic lipids complexes (lipoplexes) as mediators of its uptake in four spontaneous canine melanoma derived cell lines (Ak, Bk, Br and Rkb). Cell survival after lipofection plus or minus bleomycin was determined by the acid phosphatase method and the cellular uptake of lipoplexes carrying the E. coli B-galactosidase gene was evidenced by SYBR Green I staining.The four cell lines resulted sensitive to the bleomycin\/lipoplexes system in both spatial configurations. Survival rates values were lower than 20% in monolayers of the four tested lines and lower than 30% in three lines (Ak, Bk and Rkb) when grown as spheroids. The sensitization to bleomycin depended on lipoplexes in Ak and Rkb while Bk (in both spatial configurations) and Br (as monolayers) was sensitive to bleomycin alone. Although some degree of sensitivity to bleomycin was induced by cationic lipids alone in Ak and Rkb monolayers, the maximal bleomycin effects appeared in the presence of lipoplexes. The sensitization was independent of transcriptional activity. The co-administration of lipoplexes diminished bleomycin IC50s: 10-fold in Ak and Rkb monolayers; and sensitized the Ak and Rkb resistant spheroids. The bleomycin cytotoxic effects depended on lipoplexes concentration and diminished when cells were incubated at 8°C. Our results suggest that lipoplexes sensitize cells to bleomycin increasing its uptake by an active transport mechanism such as endocytosis. The bleomycin\/lipoplexes system appears as a promising combination of chemotherapy and non-viral cancer gene therapy.","palabras_clave":"SPHEROIDS|LIPOPLEXES|BLEOMYCIN|MELANOMA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62696,"idioma_id":4,"titulo":"rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer","resumen":"We approached the identification of Ensifer (Sinorhizobium) meliloti conjugal functions by random Tn5-B13 mutagenesis of the pSmeLPU88a plasmid of E. meliloti strain LPU88 and the subsequent selection of those mutants that had lost the ability to mobilize the small plasmid pSmeLPU88b. The Tn5-B13- insertion site of one of the mutants was cloned as an EcoRI-restricted DNA fragment that after subsequent isolation and sequencing demonstrated that a small open reading frame of 522 bp (designated rptA, for rhizobium plasmid transfer A) had been disrupted. The predicted gene product encoded by the rptA sequence shows a significant similarity to two hypothetical proteins of the plasmid pSmed03 of Ensifer medicae WSM419 and other rhizobia plasmids. No significant similarity was found to any protein sequence of known function registered in the databases. Although the rptA gene was required for pSmeLPU88b- plasmid mobilization in the strain 2011 background, it was not required in the original strain LPU88 background.","palabras_clave":"Ensifer meliloti|Plasmid|Conjugation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62697,"idioma_id":4,"titulo":"Purification and characterization of a keratinolytic serine protease from Purpureocillium lilacinum LPS # 876","resumen":"A keratinolytic serine protease secreted by Purpureocillium lilacinum (formerly Paecilomyces lilacinus)   upon culture in a basal medium containing 1% (w\/v) hair waste as carbon   and nitrogen source was purified and characterized. After purification   the keratinase was resolved by SDS-PAGE as a homogeneus protein band of   molecular mass 37.0&nbsp;kDa. The extracellular keratinase of P. lilacinum   was characterized by its appreciable stability over a broad pH range   (from 4.0 to 9.0), and up to 65&nbsp;°C, along with its strong inhibition by   phenylmethylsulphonyl fluoride among the protease inhibitors tested   (98.2% of inhibition), thus suggesting its nature as a serine protease.   The enzyme was active and stable in the presence of organic solvents   such as dimethylsulfoxide, methanol, and isopropanol; certain   surfactants such as Triton X-100, sodium dodecylsulfate, and Tween 85;   and bleaching agents such as hydrogen peroxide. These biochemical   characteristics suggest the potential use of this enzyme in numerous   industrial applications.","palabras_clave":"ENZYME PURIFICATION|KERATINASE|SERINE PROTEASE| HAIR WASTE     |PURPUREOCILLIUM LILACINUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62698,"idioma_id":4,"titulo":"A new snake skull from the Paleocene of Bolivia sheds light on the evolution of macrostomatans","resumen":"Macrostomatan snakes, one of the most diverse extant clades of squamates, display an impressive arsenal of cranial features to consume a vast array of preys. In the absence of indisputable fossil representatives of this clade with well-preserved skulls, the mode and timing of these extraordinary morphological novelties remain obscure. Here, we report the discovery of Kataria anisodonta n. gen. n. sp., a macrostomatan snake recovered in the Early Palaeocene locality of Tiupampa, Bolivia. The holotype consists of a partial, minute skull that exhibits a combination of booid and caenophidian characters, being the presence of an anisodont dentition and diastema in the maxilla the most distinctive trait. Phylogenetic analysis places Kataria basal to the Caenophidia+Tropidophiidae, and represents along with bolyeriids a distinctive clade of derived macrostomatans. The discovery of Kataria highlights the morphological diversity in the maxilla among derived macrostomatans, demonstrating the relevance of maxillary transformations in the evolution of this clade. Kataria represents the oldest macrostomatan skull recovered, revealing that the diversification of macrostomatans was well under way in early Tertiary times. This record also reinforces the importance of Gondwanan territories in the history of snakes, not only in the origin of the entire group but also in the evolution of ingroup clades.","palabras_clave":"Paleocene|Bolivia|Serpentes|Kataria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62699,"idioma_id":5,"titulo":"Nuevos registros de Hippidion (Mammalia,  Perissodactyla) en el Pleistoceno tardío de la  Provincia de Buenos Aires, Argentina","resumen":"En este estudio se describen dos nuevos cráneos referidos a Hippidion principale (Lund) y varíos elementos del esqueleto apendicular referidos a H. devillei procedentes de dos localidades clásicas de la provincia de Buenos Aires, las márgenes de los ríos Salado y Quequén Grande. Para la identificación taxonómica de los restos apendiculares, se realizó un análisis multivariante en el que se incluyeron los metacarpianos y falanges descritos y los registrados en varias localidades de Argentina y   Brasil. Para la determinación de los cráneos se realizó un estudio comparativo con otros cráneos de diversas localidades. Estos hallazgos incrementan el registro de Hippidion en América del Sur y aportan nuevas evidencias sobre la cronología de sus especies en Argentina. Se realizó una datación por AMS para el ejemplar de Río Salado (MHM-P54) que ha suministrado una edad 14C 14120 ± 50 BP. En general, los sedimentos portadores en ambas localidades son referibles a la Edad Lujanense,   Biozona de Equus (Amerhippus) neogeus.","palabras_clave":"Extinction|Mammals|Hippidion|Latest Pleistocene"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62700,"idioma_id":4,"titulo":"Sex Differences in insulin resistance in GABAB1 knock out mice","resumen":"Aims: We have previously demonstrated that the absence of functional GABA B receptors (GABABRs) disturbsglucose homeostasis in GABAB1KO mice. The aim of this work was to extend our studies of these alterationsin GABAB1KO mice and investigate the sexual differences therein.We have previously demonstrated that the absence of functional GABA B receptors (GABABRs) disturbsglucose homeostasis in GABAB1KO mice. The aim of this work was to extend our studies of these alterationsin GABAB1KO mice and investigate the sexual differences therein.Main methods: Male and female, GABAB1KO and WT mice were used. Glucose and insulin tolerance tests(GTT and ITT), and insulin and glucagon secretion tests (IST and GST) were performed. Blood glucose,serum insulin and hyperglycemic hormones were determined, and HOMA-IR calculated. Skeletal muscle insulinreceptor â subunit (IRâ), insulin receptor substrates 1\/2 (IRS1, IRS2) and hexokinase-II levels were determinedby Western blot. Skeletal muscle insulin sensitivity was assessed by in vivo insulin-induced Aktphosphorylation (Western blot). Food intake and hypothalamic NPY mRNA expression (by qPCR) werealso evaluated.Male and female, GABAB1KO and WT mice were used. Glucose and insulin tolerance tests(GTT and ITT), and insulin and glucagon secretion tests (IST and GST) were performed. Blood glucose,serum insulin and hyperglycemic hormones were determined, and HOMA-IR calculated. Skeletal muscle insulinreceptor â subunit (IRâ), insulin receptor substrates 1\/2 (IRS1, IRS2) and hexokinase-II levels were determinedby Western blot. Skeletal muscle insulin sensitivity was assessed by in vivo insulin-induced Aktphosphorylation (Western blot). Food intake and hypothalamic NPY mRNA expression (by qPCR) werealso evaluated.â subunit (IRâ), insulin receptor substrates 1\/2 (IRS1, IRS2) and hexokinase-II levels were determinedby Western blot. Skeletal muscle insulin sensitivity was assessed by in vivo insulin-induced Aktphosphorylation (Western blot). Food intake and hypothalamic NPY mRNA expression (by qPCR) werealso evaluated.Key findings: Fasted insulin and HOMA-IR were augmented in GABAB1KO males, with no alterations in females.Areas under the curve (AUC) for GTT and ITT were increased in GABAB1KO mice of both genders, indicatingcompromised insulin sensitivity. No genotype differences were observed in IST, GST or in IRâ, IRS1,IRS2 and hexokinase-II expression. Akt activation was severely impaired in GABAB1KO males while no alterationswere observed in females. GABAB1KO mice showed increased food intake and NPY expression.findings: Fasted insulin and HOMA-IR were augmented in GABAB1KO males, with no alterations in females.Areas under the curve (AUC) for GTT and ITT were increased in GABAB1KO mice of both genders, indicatingcompromised insulin sensitivity. No genotype differences were observed in IST, GST or in IRâ, IRS1,IRS2 and hexokinase-II expression. Akt activation was severely impaired in GABAB1KO males while no alterationswere observed in females. GABAB1KO mice showed increased food intake and NPY expression.â, IRS1,IRS2 and hexokinase-II expression. Akt activation was severely impaired in GABAB1KO males while no alterationswere observed in females. GABAB1KO mice showed increased food intake and NPY expression.Significance: Glucose metabolism and energy balance disruptions were more pronounced in GABAB1KOmales, which develop peripheral insulin resistance probably due to augmented insulin secretion. Metabolicalterations in females were milder and possibly due to previously described reproductive disorders, suchas persistent estrus.ficance: Glucose metabolism and energy balance disruptions were more pronounced in GABAB1KOmales, which develop peripheral insulin resistance probably due to augmented insulin secretion. Metabolicalterations in females were milder and possibly due to previously described reproductive disorders, suchas persistent estrus.","palabras_clave":"Insulin|GABA|GABAB1 Receptor|Knockout mice"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62701,"idioma_id":4,"titulo":"Numerical semi-global analysis of a 1:2 resonant Hopf-Hopf bifurcation","resumen":"In this paper, a numerical semi-global analysis of the dynamics near a 1:2 resonant Hopf?Hopf bifurcation on a four-dimensional mathematical model of a simple nonlinear oscillator is performed. The 1:2 resonant Hopf?Hopf bifurcation is a codimension-three singularity denoted by two pairs of purely imaginary eigenvalues with frequency ratio 1:2. A structure involving 1:1 and 1:2 resonant Neimark?Sacker bifurcations is clearly identified. Both resonances are coupled by lower-codimension singularities, such as generalized Hopf and period doubling, cusp points, and cyclic fold curves. A three-parameter semiglobal analysis is performed and some of the codimension-two singularities unfolded by the 1:2 resonant Hopf-Hopf bifurcation are identified. Several codimension-three points are also detected. The obtained results can be useful for further theoretical analysis of the corresponding normal form.","palabras_clave":"resonancias|doble-Hopf"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62702,"idioma_id":4,"titulo":"Reactive oxygen species produced by NADPH oxidase and mitochondrial dysfunction in lung after an acute exposure to Residual Oil Fly Ashes","resumen":"Reactive O2 species production triggered by particulate matter (PM) exposure is able to initiate oxidative damage mechanisms, which are postulated as responsible for increased morbidity along with the aggravation of respiratory diseases. The aim of this work was to quantitatively analyse the major sources of reactive O2 species involved in lung O2 metabolism after an acute exposure to Residual Oil Fly Ashes (ROFAs). Mice were intranasally instilled with a ROFA suspension (1.0mg\/kg body weight), and lung samples were analysed 1h after instillation. Tissue O2 consumption and NADPH oxidase (Nox) activity were evaluated in tissue homogenates. Mitochondrial respiration, respiratory chain complexes activity, H2O2 and ATP production rates, mitochondrial membrane potential and oxidative damage markers were assessed in isolated mitochondria. ROFA exposure was found to be associated with 61% increased tissue O2 consumption, a 30% increase in Nox activity, a 33% increased state 3 mitochondrial O2 consumption and a mitochondrial complex II activity increased by 25%. During mitochondrial active respiration, mitochondrial depolarization and a 53% decreased ATP production rate were observed. Neither changes in H2O2 production rate, nor oxidative damage in isolated mitochondria were observed after the instillation. After an acute ROFA exposure, increased tissue O2 consumption may account for an augmented Nox activity, causing an increased O2(-) production. The mitochondrial function modifications found may prevent oxidative damage within the organelle. These findings provide new insights to the understanding of the mechanisms involving reactive O2 species production in the lung triggered by ROFA exposure","palabras_clave":"Air pollution|Lung|Mitochondria|NADPH oxidase|Residual oil fly ash (ROFA)|Reactive O2 species"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62703,"idioma_id":4,"titulo":"Nutrients and abiotic stress interact to control ergot plant disease in a SW Atlantic salt marsh","resumen":"                Over the last decades human activities have strongly affected  ecosystems, with pervasive increases in nutrient loadings, abiotic stress and  altered herbivore pressure. The evaluation of how those environmental factors interact  to influence plant-pathogen interactions under natural conditions becomes  essential to fully understand the ecology of diseases and anticipate the  possible effects of global change on natural and agricultural systems. In a SW  Atlantic salt marsh we performed a field factorial experiment to evaluate  the effect of herbivory, salinity and nutrient availability, three main  limiting factors for salt marsh plant growth, on the infection of the fungus Claviceps purpurea (ergot) upon the cordgrass  Spartina densiflora. Results show  that herbivory has no effect but both nutrients and salinity increase fungal  infection. The combined effect of salinity and nutrients is not additive but  interactive. Salinity stress increases  infection at ambient nutrient levels but in combination with fertilizer it  buffers the higher infection produced by increased nutrient availability. Since both, nitrogen  availability and salinity are factors predicted to globally increase due to  human impact on ecosystems, this interaction between environmental factors and  ergot infection can have strong effects on natural and productive agricultural  systems.&nbsp;&nbsp;&nbsp;   ","palabras_clave":"PLANT-PATHOGEN INTERACTIONS|ERGOT|SALT MARSH|NITROGEN|SALINITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62704,"idioma_id":4,"titulo":"Effect of natural and synthetic antioxidants on the oxidative stability of walnut oil under different storage conditions","resumen":"    The aim of the  work was to evaluate the combined effects of the storage condition (six months  under fluorescent light \u2013 800 Lux - or darkness condition, both at room  temperature) with the addition of natural (rosemary extract, RE) and synthetic (ascorbyl  palmitate \u2013 AP -, and Tert-butylhidroquinone - TBHQ) antioxidants on quality  indices related to walnut oil (WO) oxidative stability. Neither RE nor synthetic antioxidants contributed  markedly on inhibiting photo-oxidative degradation, resulting in significantly  increased amounts of primary and secondary oxidation products in oils exposed  to light. Under darkness-storage condition, the addition of the mentioned  antioxidants significantly reduced lipid oxidation and improved oil shelf-life.  Oils added with RE - alone or in combination with TBHQ or TBHQ plus AP \u2013  maintain an acceptable quality at least up to six month of storage. Results from  this work stressed the influence of the illumination condition on WO oxidative  stability, suggesting that this oil should be stored in containers with  light-barrier properties, and may be added with the antioxidants examined in  the current study.  ","palabras_clave":"Oil nut|antioxidant"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62705,"idioma_id":4,"titulo":"Reduction and catalytic behaviour of nanostructured Pd\/gadolinia-doped ceria catalysts for methane combustion","resumen":"In the present work,  1 wt% and 5 wt% Pd\/GDC10 (Gd0.1Ce0.9O1.95)  nanopowders were prepared by two different methods: a) cation complexation (CC)  and b) incipient wetness impregnation (WI) of an aqueous Pd2+  solution onto GDC10 nanopowders. All samples were characterized by X-ray  diffraction (XRD), thermal analysis (TG\/DTA), specific surface area  determination and high resolution transmission electron microscopy (HRTEM). In  order to study the oxidation state of Pd in samples with and without reducing  treatments, XANES experiments at the Pd L3-edge were carried out. In  situ Ce L3-edge XANES experiments were performed under reducing  conditions in order to investigate the reduction behavior of these materials. The  addition of Pd to the GDC10 nanopowders increased the reducibility of Ce in the  mixed oxide. Finally, catalytic tests for CH4 combustion were  performed on the Pd\/GDC10 nanopowders. Catalysts with higher Pd loading (5 wt%)  exhibited the best performance for CH4 combustion. The influence of  preparation method was evident for catalysts with 1 wt% Pd, the cation  complexation method resulting in more active catalysts than the wetness  impregnation method.      ","palabras_clave":"Ceria Gadolinia|Pd|Redox|Ch4 Combustion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62706,"idioma_id":4,"titulo":"Seasonal variability and coastal upwelling near Cape Santa Marta (Brazil)","resumen":"The Santa Marta Grande Cape (CSM) area, a relatively steep and narrow continental shelfoff southeast Brazil, is under the influence of the Brazil Current and intense seasonal variations of the wind field that affect the composition of water masses and their circulation. Hydrographic data, satellite derived wind stress and numerical simulations are used to characterize the seasonal and shorter period variability of the oceanographic conditions off CSM, in the area between 27ºS and 30ºS. The study is focused on the upwelling episodes and the dynamical mechanisms associated with these events. Field data and numerical results indicate significant seasonal changes in the oceanographic structure, the water mass composition and the dynamical balance. During fall and winter, when southwesterly winds prevail and the frequency of meteorological frontal systems increases the region is characterized by waters of continental origin, the Plata Plume Water (PPW). During spring and summer deeper waters reach the surface layer due to the action of northeasterly winds. Coastal upwelling events are revealed by surface temperature minima observed south of CSM. Synoptic data show that these events occur in pulses associated with changes in the wind direction. The analysis of the numerical results show that this particular location of the upwelling band results from the synergy between shelf-break upwelling and the regional shelf circulation induced by the northeasterly winds both of which are modulated by changes in the coastline orientation and shelf width.","palabras_clave":"upwelling,|Cape Santa Marta|wind effects|dynamical balance"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62707,"idioma_id":4,"titulo":"Morphosedimentary and hydrographic features of the northern Argentine margin: the interplay between erosive, depositional and gravitational processes and its conceptual implications","resumen":"Bottom currents and their margin-shaping character became a central aspect in the researchfield of sediment dynamics and paleoceanography during the last decades due to their potential to form large contourite depositonal systems (CDS), consisting of both erosive and depositional features. A major CDS at the northern Argentine continental margin was studied off the Rio de la Plata River by means of seismo- and hydro-acoustic methods including conventional and high-resolution seismic, parametric echosounder and single and swath bathymetry. Additionally, hydrographic data were considered allowing jointly interpretation of morphosedimentary features and the oceanographic framework, which is dominated by the presence of the dynamic and highly variable Brazil-Malvinas Confluence.We focus on three regional contouritic terraces identified on the slope in the vicinity of the Mar del Plata Canyon. The shallowest one, the La Plata Terrace (~500 m), is located at the Brazil Current\/Antarctic Intermediate Water interface characterized by its deep and distinct thermocline. In ~1200 m water depth the Ewing Terrace correlates with the Antarctic Intermediate Water \/Upper Circumpolar Deep Water interface. At the foot of the slope in ~3500 m the Necochea Terrace marks the transition between Lower Circumpolar Deep Water and Antarctic Bottom Water during glacial times. Based on these correlations, a comprehensive conceptual model is proposed, in which the onset and evolution of contourite terraces is controlled by short- and long-term variations of water mass interfaces. We suggest that the terrace genesis is strongly connected to the turbulent current pattern typical for water mass interfaces. Furthermore, the erosive processes necessary for terrace formation are probably enhanced due to internal waves, which are generated along strong density gradients typical for water mass interfaces. The terraces widen through time due to locally focused, partly helical currents along the steep landward slopes and more tabular conditions seaward along the terrace surface.&nbsp; Considering this scheme of contourite terrace development, lateral variations of the morphosedimentary features off northern Argentina can be used to derive the evolution of the Brazil-Malvinas Confluence on geological time scales. We propose that the Brazil-Malvinas Confluence in modern times is located close to its southernmost position in the Quaternary, while its center was shifted northward during cold periods.","palabras_clave":"contourite drift|contourite terraces|water mass interfaces|erosive surface|Brazil- Malvinas Confluence"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62708,"idioma_id":4,"titulo":"Behavioural responses of argentine coastal dusky dolphins, Lagenorhynchus obscurus, to a biopsy pole system","resumen":"In recent years, analysis of tissue samples has become a powerful tool in cetacean ecology since it provides information for effective conservation and management policies. Biopsy samples taken for such studies have been obtained by various means including crossbows, rifles, and pole systems. We investigated sampling success and the reaction of dusky dolphins (Lagenorhynchus obscurus) off the coast of Argentina to a biopsy pole system from 2008 to 2009. Using ad libitum behavioural sampling, we documented individual behavioural reactions to biopsy sampling immediately after a biopsy was taken. Log-linear models were used to determine whether there were significant differences in behavioural response for different group size, composition or behavioural context. In total 109 sampling attempts were made and 58 biopsy samples were obtained (53.21 % success rate). The intensity of individual response was influenced by group size and composition. Small groups (typically mother\/calf) reacted more frequently than larger groups (generally mixed age adults and juveniles). The behaviour of the animals prior to our approach for biopsy sampling also affected their response. Milling dolphins appeared to respond more intensively than dolphins socializing or travelling. In conclusion, biopsy sampling with the biopsy pole system presented here show generally mild and short-term reactions, though behaviour, group size and group composition affected the response. This information can be used to minimise the impact of biopsy sampling, and facilitate the collection of data critical to effective conservation strategies.provides information for effective conservation and management policies. Biopsy samples taken for such studies have been obtained by various means including crossbows, rifles, and pole systems. We investigated sampling success and the reaction of dusky dolphins (Lagenorhynchus obscurus) off the coast of Argentina to a biopsy pole system from 2008 to 2009. Using ad libitum behavioural sampling, we documented individual behavioural reactions to biopsy sampling immediately after a biopsy was taken. Log-linear models were used to determine whether there were significant differences in behavioural response for different group size, composition or behavioural context. In total 109 sampling attempts were made and 58 biopsy samples were obtained (53.21 % success rate). The intensity of individual response was influenced by group size and composition. Small groups (typically mother\/calf) reacted more frequently than larger groups (generally mixed age adults and juveniles). The behaviour of the animals prior to our approach for biopsy sampling also affected their response. Milling dolphins appeared to respond more intensively than dolphins socializing or travelling. In conclusion, biopsy sampling with the biopsy pole system presented here show generally mild and short-term reactions, though behaviour, group size and group composition affected the response. This information can be used to minimise the impact of biopsy sampling, and facilitate the collection of data critical to effective conservation strategies.","palabras_clave":"animal welfare|biopsy pole system|behavioural response|dusky dolphins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62709,"idioma_id":4,"titulo":"A novel Interleukin-10 DNA mucosal delivery system attenuates intestinal inflammation in a mouse model","resumen":"Inflammatory bowel diseases (IBD) describe a group of complex intestinal disorders characterized by inflammation in the gastrointestinal tract. Current treatments for IBD include the use of anti-inflammatory drugs; furthermore, recombinant lactic acid bacteria have been used as a therapeutic vehicle for anti-inflammatory agents in IBD models. Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokines; however, its oral administration is limited because it is quickly degraded in the gastrointestinal tract and systemic treatments have led to undesirable side effects. In this study, an engineered invasive strain of Lactococcus (L.) lactis producing Fibronectin Binding Protein A (FnBPA+), from Staphylococcus aureus capable of delivering, directly inside eukaryotic cells, an eukaryotic DNA expression vector containing the ORF coding for IL-10 of Mus musculus (pValac:il-10) was developed and its functionality was evaluated using in vitro and in vivo assays. Functionality of the plasmid and the invasive strain was demonstrated by transfection and invasiveness assays using cell cultures and in vivo in mice by fluorescence microscopy. TNBS inoculated mice that received this novel strain showed lower damage scores in their large intestines (at both macroscopic and microscopic levels), lower microbial translocation to liver, and increased anti-inflammatory\/pro-inflammatory cytokine ratios compared to mice that received L. lactis FnBPA+ without the pValac:il-10 plasmid. The effectiveness was demonstrated of this novel DNA delivery therapeutic strategy in the prevention of inflammation using a murine model of colitis.","palabras_clave":"INTERLEUKIN-10|CROHN'S DISEASE|DNA DELIVERY SYSTEM|LACTOCOCCUS LACTIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62710,"idioma_id":4,"titulo":"Mucosal targeting of therapeutic molecules using genetically modified lactic acid bacteria: an update","resumen":"Lactic acid bacteria (LAB) represent a heterogeneous group of microorganisms naturally present in many foods and those have proved to be effective mucosal delivery vectors. Moreover, some specific strains of LAB exert beneficial properties (known as probiotic effect) on both human and animal health. Although probiotic effects are strain-specific traits, it is theoretically possible, using genetic engineering techniques, to design strains that can exert a variety of beneficial properties. During the two past decades, a large variety of therapeutic molecules has been successfully expressed in LAB, and although this field has been largely reviewed in recent years, approximately 20 new publications appear each year. Thus, the aim of this minireview is not to extensively assess the entire literature but to update progress made within the last 2 years regarding the use of the model LAB Lactococcus lactis and certain species of lactobacilli as live recombinant vectors for the development of new safe mucosal vaccine.","palabras_clave":"LACTIC ACID BACTERA|MUCOSAL VACCINES|CYTOKINES|THERAPEUTIC MOLECULES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62711,"idioma_id":4,"titulo":"Effect of a probiotic fermented milk on the thymus in Balb\/c mice under non-severe protein-energy malnutrition","resumen":"Protein-energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB\/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4+\/CD8- single-positive thymocytes. Immature double-positive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model.","palabras_clave":"FERMENTED MILK|NON-SEVERE  PROTEIN-ENERGY MALUTRITION|PROBIOTIC|THYMUS|MOUSE MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62712,"idioma_id":4,"titulo":"Mechanisms involved in the anti-inflammatory properties of native and genetically engineered lactic acid bacteria","resumen":"Lactic acid bacteria (LAB) represent a heterogeneous group of microorganisms that have been shown to possess therapeutic properties since they are able to prevent the development of some diseases, as shown mostly on animal models for cancer, infections and gastrointestinal disorders such as intestinal inflammation. LAB have been shown to regulate mucosal immune responses by modulating the production and liberation of regulatory agents such as cytokines by the host. Some of these cytokines, such as the anti-inflammatory interleukin-10 (IL-10), modulate the inflammatory immune response, thus immunomodulation is a mechanism by which LAB can prevent certain inflammatory bowel diseases (IBD). Since oxidative stress participates to the inflammatory processes and to the appearance of damages in pathologies of the gastrointestinal tract of humans such as IBD, LAB could also prevent inflammation by eliminating reactive oxygen species (ROS) through the activity of antioxidant enzymes.In order to obtain novel strains or enhance beneficial effects of LAB, genetic engineering has been used to produce either antioxidant enzymes (such as catalases and superoxide dismutases) or anti-inflammatory cytokines (such as IL-10) producing LAB. These novel strains have successfully been used to prevent inflammatory bowel diseases in animal models and could be evaluated in human clinical trials. Here, we present an overview of the current knowledge of the mechanisms by which LAB can be used to prevent undesired intestinal inflammatory responses and could be used as a therapeutic tool for IBD.","palabras_clave":"LACTIC ACID BACTERIA|ANTI-INFLAMMATORY|ANTIOXIDANT |INTERLEUKIN-10"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62713,"idioma_id":4,"titulo":"Determination of tributyltin at parts-per-trillion levels in natural waters by second-order multivariate calibration and fluorescence spectroscopy","resumen":"This work presents a non-sophisticated approach for the trace determination of tributyltin, the most toxic organotin species, in very interfering environments, combining fluorescence measurements of its morin complex and the selectivity of second-order chemometric algorithms. The power of MCR?ALS (multivariate curve resolution\/alternating least-squares) to quantify tributyltin through fluorescence excitation?emission matrices in the presence of its main degradation products and of a pool of additional twenty-three metalions is demonstrated. The applied algorithm successfully faces the challenge of solving the strong overlapping among the spectra of the several sample components. The proposedmethodologywas applied to tap, river, lagoon and seawater spiked samples, obtaining satisfactory results at ng L&#8722;1 levels, after a pre-concentration step on a C18 membrane, demonstrating the analytical potential of the proposed methodology.","palabras_clave":"TRIBUTYLTIN|NATURAL SAMPLES|MULTIVARIATE CALIBRATION|SPECTROFLUORIMETRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62714,"idioma_id":4,"titulo":"Feasibility of the determination of polycyclic aromatic hydrocarbons in edible oils via unfolded partial least-squares\/residual bilinearization and parallel factor analysis of fluorescence excitation emission matrices","resumen":"    The possibility of simultaneously determining seven concerned  heavy polycyclic aromatic hydrocarbons (PAHs) of the US-EPA priority pollutant list,  in extra virgin olive and sunflower oils was examined using unfolded partial least-squares  with residual bilinearization (U-PLS\/RBL) and parallel factor analysis (PARAFAC).  Both of these methods were applied to fluorescence excitation emission matrices.  The compounds studied were benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene,dibenz[a,h]anthracene, benzo[g,h,i]perylene and indeno[1,2,3-c,d]-pyrene. The analysis was  performed using fluorescence spectroscopy after a microwave assisted liquid\u2013liquidextractionand  solid-phase extraction on silica. The U-PLS\/RBL algorithm exhibited the best performance  for resolving the heavy PAH mixture in the presence of both the highly complex oil  matrix and other unpredicted PAHs of the US-EPA list. The obtained limit of detection  for the proposed method ranged from 0.07 to 2 mg\/kg. The predicted U-PLS\/RBL concentrations  were satisfactorily compared with those obtained using high-performance liquid chromatography  with fluorescence detection. A simple analysis with a considerable reduction in  time and solvent consumption in comparison with chromatography are the  principal advantages of the proposed method.  ","palabras_clave":"Polycyclic aromatic hydrocarbons|Edible oils|Fluorescence excitation-emission matrices|Multivariate calibration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62715,"idioma_id":4,"titulo":"Crystal structure and spectroscopic properties of N- methoxycarbonylsulfenyl-substituted ureas, CH3OC(O)SN(H)C(O)NRR´ [R = H, R´ = C(CH3)3 and R = R´ = CH2CH3]","resumen":"Structural and spectroscopic properties of N,N-diethyl- (I) and N-tert-butyl-N\u2019-methoxycarbonylsulfenyl urea (II) are analyzed using a combined approach with data obtained from X-ray diffraction, vibrational spectra and quantum chemical calculations. The molecular structures of CH3OC(O)SN(H)C(O)N(CH2CH3)2 (I) and CH3OC(O)SN(H)C(O)N(H)C(CH3)3 (II), the later co-crystallized with ethyl acetate (EtAc) solvent molecule to give the adduct&nbsp; II.½EtAc, were determined by X-ray diffraction methods. were determined by X-ray diffraction methods. Compound I crystallizes in the triclinic P-1 space group with a=7.9471(4), b=8.6672(4), c=16.0447(8) Å, a=80.335(4), b=87.087(4), g=75.271(4)º, and Z=4 molecules per unit cell and II.½EtAc in the orthorhombic Pnma with with a=9.365(5), b = 26.577(5), c = 10.287(5) Å, and Z=8. The structures were solved from 3377 (I) and 1614 (II.½EtAc) reflections with I&gt;2s(I) and refined to agreement R1-factors of 0.0410 (I) and 0.0441 (II.½EtAc). In the I solid there are two independent but closely related molecules in the crystal asymmetric unit whose conformations differ only in the chirality of the terminal -N(CH2-CH3)2 groups. Neighboring I molecules are N-H\u2026O bonded to each other giving rise to a polymeric arrangement. The conformation of the O-(C=O)-S-N-(C=O)-N skeleton of II in the II.½EtAc solid is nearly the same as the corresponding one of both 1 molecules. Neighboring II molecules in the lattice are linked through a bifurcated N-H\u2026O\u2026H-N bonds giving rise to a polymeric chain. The vibrational properties have been studied by FTIR and FT-Raman spectroscopy along with quantum chemical calculations at the B3LYP\/6-311+G* level.","palabras_clave":"MOLECULAR STRUCTURE|CONFORMATION|X-RAY|UREA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62716,"idioma_id":4,"titulo":"Spectroscopic Characterization, Constitutional and Rotational Isomerism of ClC(O)SCN and ClC(O)NCS","resumen":"Chlorocarbonylthio- and isothiocyanate (ClC(O)SCN and ClC(O)NCS) have been isolated and characterized by IR (Ar matrix, gas), Raman (liquid), 13CNMR and UV&#8722;visible spectroscopies. Vibrational and quantum chemical studies suggest the presence of the syn and anti conformers (SCN group with respect to the C=O bond) in the gas phase for both constitutional isomers. syn-ClC(O)SCN is preferred by &#916;H° (anti\/syn) = 1.3(0.3) kcal mol&#8722;1. The solid-state structure of ClC(O)SCN has been determined by single crystal X-ray diffraction analysis at low temperature. The crystalline solid consists exclusively of molecules in the syn conformation. On the other hand, the anti form is more stable for the ClC(O)NCS isomer. The structure of ClC(O)NCS and its conformational composition were determined by gas electron diffraction. An unusual low syn &#8594; anti interconversion energy barrier of 0.98 (0.15) kcal mol&#8722;1 was detected for&nbsp; ClC(O)NCS at cryogenic temperatures. The photochemistry of both constitutional isomers isolated in solid argon at 15 K was studied. Rearrangement of ClC(O)SCN to ClC(O)NCS was observed in the neat liquid and under UV&#8722;vis irradiation of ClC(O)SCN isolated in solid argon. Properties have been discussed in terms of the valence electronic structure, including the analysis of the He(I) photoelectron spectrum of ClC(O)SCN","palabras_clave":"ISOCYANATE|THIOISOCYANATE|CONFORMATION|MOLECULAR STRUCTURE|MATRIX ISOLATION|X-RAY DIFFRACTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62717,"idioma_id":4,"titulo":"A different method to update monthly median hmF2 values","resumen":"The height, hmF2, and the electron density, NmF2, of the F2 peak are key model parameters to characterize the actual state of the  ionosphere. These parameters, or alternatively the propagation factor, M3000F2, and the critical frequency, foF2, of the F2 peak, which  are related to hmF2 and NmF2, are used to anchor the electron density vertical profile computed with different models such as the International  Reference Ionosphere (Bilitza, 2002), as well as for radio propagation forecast purposes. Long time series of these parameters  only exist in an inhomogeneous distribution of points over the surface of Earth, where dedicated instruments (typically ionosondes) have  been working for many years. A commonly used procedure for representing median values of the aforementioned parameters all over the  globe is the one recommended by the ITU-R (ITU-R, 1997). This procedure, known as the Jones and Gallet mapping technique, was  based on ionosondes measurements gathered from 1954 to 1958 by a global network of around 150 ionospheric stations (Jones and Gallet,  1962; Jones and Obitts, 1970). Even though several decades have passed since the development of that innovative work, only few  efforts have been dedicated to establish a new mapping technique for computing hmF2 and NmF2 median values at global scale or to  improve the old method using the increased observational database. Therefore, in this work three different procedures to describe the  daily and global behavior of the height of the F2 peak are presented. All of them represent a different and simplified method to estimate  hmF2 and are based on different mathematical expressions. The advantages and disadvantages of these three techniques are analyzed,  leading to the conclusion that the recommended procedure to represent hmF2 is best characterized by a Spherical Harmonics expansion  of degree and order equal to 15, since the differences between the hmF2 values obtained with the Jones and Gallet technique and those  obtained using the abovementioned procedure are of only 1%.","palabras_clave":"hmF2|Electron Density Profile|ITU-R Database|Ionosphere"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62718,"idioma_id":4,"titulo":"Symbiotic interactions as drivers of trade-offs in plants: effects of fungal endophytes on tall fescue","resumen":"          Studying  the controls on biomass allocation trade-offs in plants are important since  they affect harvestable product yields and are critical to understanding  symbiotic interactions. Epichloae fungal endophytes associate with cool-season  grasses, growing systemically within the plant inter-cellular spaces and are  transmitted through seeds. We explore the endophytes influence on the  relationship between the plant reproductive and vegetative aboveground biomass  (reproductive effort: RE) and on the trade-off between two components of the  reproductive biomass, number and weight of panicles (RPN), using tall fescue as  a model system. Naturally endophyte-colonized, manipulatively endophyte-free,  and naturally endophyte-free plants from Northern European wild-populations  together with the cultivar Kentucky-31 were grown under different environmental  conditions (nutrients x water). The endophyte had an effect on the RPN (E+:  6.19, ME-: 4.68 and E-: 4.40) which indicates how reproductive biomass is  partitioned into number and mass of panicles, but not on RE (&#8776;0.06). As  expected, wild plants showed higher reproductive effort (&#8776;0.06) compared to the  cultivar KY-31 (0.05), irrespective of endophyte presence. Endophyte-colonized  plants had lighter panicles than endophyte-free plants, a pattern that was  clear among low-yielding plants. Similarly, the trade-off between RPN and RE  was higher for endophyte-colonized plants. This was again evident among plants  with low RE indicating that colonized plants split the yield into either  greater number of panicles and\/or lighter panicles. The effect of vertically  transmitted endophytes has earlier been studied as ratios (e.g. RE); however,  our study shows that this approach may hide size-dependent endophyte effects on  these relationships. Our study reveals that Neotyphodium endophyte affects  trade-offs in tall fescue plants in a complex manner, and is influenced by a  number of biological and abiotic factors.&nbsp;  ","palabras_clave":"Allometry|Biomass partitioning|Neotyphodium|Festuca"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62719,"idioma_id":4,"titulo":"'LDA + U and GGA + U studies of Al-rich and bulk goethite (alfa-FeOOH)'","resumen":"The electronic and structural properties of bulk goethite and Al-rich goethite were studied on the basis of spin-polarized DFT at the LDA+U and GGA(PW91)+U levels. Firstly, the periodic model of bulk goethite was optimized varying the value of Ueff. Considering all the results obtained we can conclude that the bulk goethite described at the GGA+U level with Ueff = 6 eV gives us the better agreement with different physical properties. The results of magnetic moments of Fe ions, the DOS analysis and the Bader atomic charges identify goethite as an antiferromagnetic Fe(III) compound. For Al-rich goethite the GGA+U (Ueff = 6 eV) approach was used. The isomorphous substitution of one Fe ion with Al ion produces the reduction of the cell parameters with respect to the bulk goethite. Regarding the magnetic ordering, it was observed that Fe atoms surrounding the Al atom must have the same spin projection, i.e., spin up or down. The charge density was changed with the addition of Al ion, producing a depletion where the ion is located and some electron redistribution in the zone of the oxygen atoms surrounding the Al ion. This behavior produces some small magnetization in these O ions.","palabras_clave":"BULK|GOETHITA|DFT|STRUCTURES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62720,"idioma_id":4,"titulo":"Grassland afforestation impact on primary productivity: a remote sensing approach","resumen":"Question: How is the magnitude and seasonality of carbon uptake affected by the replacement of native grasslands by eucalyptus plantations?  Location: Rio de la Plata Grasslands in Argentina and Uruguay.  Methods: A total of 115 paired sites of fast-growing Eucalyptus grandis plantations and adjacent grasslands were used to characterize the magnitude and seasonality of (1) radiation interception by canopies and (2) above-ground net primary productivity based on a time series of MODIS-derived normalized difference vegetation index (NDVI). The response of NDVI to precipitation was explored across temporal scales.  Results: NDVI in afforested vs. grassland plots presented higher annual averages (1.3-fold), lower seasonal ranges (average relative range of 0.11 vs. 0.29) and delayed growing seasons (2-month shift). Temporally, NDVI was positively associated with precipitation input, showing a correlation with longer periods of precipitation accumulation in tree plantations compared to grasslands (> 7 vs. 2.3 months). Estimated average annual above-ground net primary productivity (ANPP) almost quadrupled as a consequence of replacing grasslands by tree plantations  (¡­4 vs. ¡­17 Mg drymatter. ha-1 yr-1), and this difference was evidenced  throughout thewhole study period.  Conclusions: Afforested grasslands intercept more radiation and have higher andmore stable ANPP throughout the year, probably as a result of major changes in leaf phenology and root distribution patterns, which in turn allowed better access towater. Changes in carbon uptake can influence climate\/biosphere feedbacks and should be considered in land-use planning, especially when grassland afforestation is recommended as a tool to mitigate global warming.","palabras_clave":"ANPP|Land-use change|MODIS|NDVI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62721,"idioma_id":5,"titulo":"Una nueva guía de aves para el centro de la argentina.","resumen":"DE LA PEÑA MR Y TITTARELLI RF (2011) Guía de aves de La Pampa. Gobierno de La Pampa, Santa Rosa. 296pp. ISBN 978-987-23239-3-6 (rústica)","palabras_clave":"Revisión Guía de Aves"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62722,"idioma_id":4,"titulo":"Refuge effect of an unpalatable forb on community structure and grass morphology in a temperate grassland","resumen":"The role of unpalatable plant species as biological antiherbivore refuges for palatable species is well-documented at community level particularly in harsh environments. In productive sub-humid temperate grassland subjected to domestic grazing, we examined the protective effect of Eryngium horridum on plant community structure and floristic composition, and evaluated whether these changes impacted on a number of morphological traits of grasses, related to grazing resistance. We also investigated, for a palatable grass species (Stipa neesiana) the existence of morphological differences between protected and unprotected plants and if this eventual variation was either plastic or genetic. The study consisted of a field survey where we compared paired patches, with and without E. horridum, and a greenhouse experiment where we evaluated individuals of S. neesiana coming from both patch types over a 11 months period. Patches dominated by E. horridum had lower richness and cover of forbs than patches without the forb, and similar richness but greater cover of cool-season tussock palatable grasses, which suggests a protective role on the latter. Grasses in these patches also had longer blades and sheaths and lower specific leaf area. The morphological differences of S. neesiana individuals collected from both patch types disappeared after 11 months growth in a common environment which revealed significant phenotypic plasticity in this species. These results suggest the existence of plant-to-plant facilitation in a productive ecosystem not only at community level, through changes in species richness and the promotion of palatable grasses, but also at population level, through plastic changes in aboveground morphological traits. Both facilitation and plasticity, would contribute to the persistence of threatened palatable grasses in the heavy grazed productive ecosystems.","palabras_clave":"Eryngium horridum|indirect facilitation|plan-plant interactions|phenotypic plasticity|positive relationships|Stipa neesiana"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62723,"idioma_id":4,"titulo":"The functional and numerical responses of Trissolcus basalis (Hymenoptera: Platygastridae) parasitizing Nezara viridula (Hemiptera: Pentatomidae) eggs in the field","resumen":"    ABSTRACT Trissolcus basalis has been used  as a biological control agent of its main host, Nezara viridula, in many  countries. However, estimations of its functional and numerical responses in  the field are lacking. We estimated the density of N. viridula eggs, the  proportion of parasitized N. viridula eggs, and the number of T. basalis  adults\/trap in the field. We transformed relative parasitoid density to an  absolute density, and estimated the parasitoid´s attack rate, a, and the mutual  interference parameter, m, in two ways: following Arditi &amp; Akçakaya (1990),  and using the Hassell-Varley-Holling model with two iterative techniques. The  attack rate estimated by both methods were a=1.097 and a=0.767, respectively. Parameter  m varied less between methods: m=0.563 and m=0.586, respectively and when used  to calculate the number of parasitized N. viridula eggs\/m², differences with  observed values were not significant. The numerical response of T. basalis was  affected by the sex allocation of their progeny, and the proportion of trapped  adult parasitoids decreased with field parasitoid population density.  Theoretical models show that 0&lt;m&lt;27 1 is a stabilizing factor, and  previous re-analysis of field data showed on average m=0.8. The  Holling-Hassell- Varley model leads to a flexible description of the functional  response allowing to predict acceptable weekly host parasitism. The preimaginal  parasitoid survival and the change in sex ratio as a function of parasitoid  density adequately describe the numerical functional response of the  parasitoid.   ","palabras_clave":"PEST|BIOLOGICAL CONTROL|POPULATION DYNAMICS|PARASITISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62724,"idioma_id":5,"titulo":"Propuesta para el uso exclusivo de la tasa interna de retorno modificada en la toma de decisión de proyectos industriales de inversión","resumen":"La TIR (tasa interna de retorno) es el método de evaluación de rentabilidad de proyectos de inversión mejor conocido. Esta tasa interna de retorno representa, en la interpretación tradicional de las tasas de interés,  a la tasa de interés ganada por una inversión alternativa sobre el saldo no recuperado de una inversión. Sin embargo, cuando se presenta una inconsistencia, basada en la aparición de raíces múltiples, se acude a la Tasa Interna de Retorno Modificada (TIRM) para aportar una definición a la toma de decisiones. El trabajo sostiene que este método de la TIRM puede ser utilizado siempre en la toma de decisiones económicas financieras,  porque conceptualmente utiliza valores en sus variables de decisión, mucho más cercanos a los que operan en el mercado y en el conocimiento de quién toma la decisión, involucrando en su resultado el que se puede obtener con los métodos tradicionales, en el caso que estos se aplican con solvencia. Aún, este método se comprobará es efectivo en el caso de proyectos de inversión con diferentes horizontes de planificación.","palabras_clave":"tasa interna de retorno|Tasa Interna de Retorno Modificada|evaluación de rentabilidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62725,"idioma_id":4,"titulo":"Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells","resumen":"We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.","palabras_clave":"Baculovirus|Nucleopolyhedrovirus|Spodoptera |Lefs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62726,"idioma_id":4,"titulo":"Multi-locus typing scheme for Babesia bovis and Babesia bigemina reveals high levels of genetic variability in strains from Northern Argentina","resumen":"Bovine babesiosis, caused by the protozoa Babesia bovis and Babesia bigemina, is a tick-borne disease distributedin tropical regions worldwide. Current control measures are based on the use of acaricides andlive attenuated vaccines. The major economic impact of babesiosis lies in the cattle industry.In order to gain insight into the extent of genetic diversity in populations of parasites in the field, wedeveloped two MLST schemes for the molecular genotyping of B. bigemina and B. bovis. We have alsodeveloped a custom-designed bioinformatic pipeline to facilitate the automated processing of rawsequences and further diversity and phylogenetic analysis.The overall MLST scheme exhibited the maximum discriminatory power (Simpson Index = 1) for B.bovis and a high level of discrimination for B. bigemina (Simpson Index = 0.9545). Genetic diversity wasvery high and infections with multiple genotypes were frequently found for both parasites in outbreaksamples from the Northeast and Northwest of Argentina. Recombination events, which could have arisenfrom these multiple infections, were suggested by intra-loci linkage disequilibrium analysis and the lackof congruence in phylogenetic trees from individual genes.The two MLST schemes developed here are a robust, objective and easily adoptable technology to analyzethe genetic diversity and population structure of parasites of the genus Babesia","palabras_clave":"Babesia bovis|Babesia bigemina|MLST|Argentina|Phylogeny|Genetic diversity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62727,"idioma_id":4,"titulo":"'Electron Transfer Properties of Dual Self-Assembled Architectures Based on Specific Recognition and Electrostatic Driving Forces: Its Application to Control Substrate Inhibition in Horseradish Peroxidase-Based Sensors'","resumen":"This work describes the synergistic combination of ionic self-assembly and recognition-directed assembly with the aim of creating highly functional bioelectrochemical interfaces compatible with the supramolecular design of a wide variety of biosensing platforms. A recently synthesized glycopolyelectrolyte constituted of polyallylamine bearing redox-active osmium complexes and glycosidic residues (lactose) is used to create a self-assembled structure with sodium dodecylsulfate. In turn, this supramolecular thin films bearing redox-active and biorecognizable carbohydrate units enable the facile assembly of functional lectins as well as the subsequent docking and \u201Cwiring\u201D of glycoenzymes, like horseradish peroxidase (HRP) (an elusive enzyme to immobilize via noncovalent interactions). The assembly of this system was followed by quartz crystal microbalance and grazing incidence small-angle X-ray scattering (GISAXS) studies confirming that spin-coated ionically self-assembled films exhibit mesostructured architectures according to the formation of self-organized lamellar structures. In-depth characterization of theelectrocatalytic properties of the biosupramacromolecular assemblies confirmed the ability of this kind of interfacial architecture to efficiently mediate electron transfer processes between the glycoenzyme and the electrode surface. For instance, our experimental electrochemical evidence clearly shows that tailor-made interfacial configurations of the ionic self-assemblies can prevent the inhibition of the glycoenzyme (typically observed in HRP) leading to bioelectrocatalytic currents up to 0.1 mA cm&#8722;2. The presence of carbohydrate moieties in the ionic domains promotes the biorecognition-driven assembly of lectins adding a new dimension to the capabilities of ionic self-assembly.","palabras_clave":"electron transfer|signal transduction|soft matter"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62728,"idioma_id":4,"titulo":"Classification of Argentinean Sauvignon Blanc Wines by UV Spectroscopy and Chemometric Methods","resumen":"Argentina is an important worldwide wine producer. In this country, there are several recognizable provinces that produce Sauvignon blanc wines: Neuquén, Río Negro, Mendoza, and San Juan. The analysis of the provenance of these white wines is complex and requires the use of expensive and time-consuming techniques. For this reason, this work discusses the determination of the provenance of Argentinean Sauvignon blanc wines by the use of UV spectroscopy and chemometric methods, such as principal component analysis (PCA), cluster analysis (CA), linear discriminant analysis (LDA), and partial least square discriminant analysis (PLS-DA). The proposed method requires low-cost equipment and short-time analysis in comparison with other techniques. The results are in very good agreement with results based on the geographical origin of Sauvignon blanc wines.","palabras_clave":"CLASSIFICATION|SAUVIGNON|BLANC|CHEMOMETRICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62729,"idioma_id":4,"titulo":"Two new species and a new genus of Calloporidae (Bryozoa: Cheilostomata) from the Southwest Atlantic","resumen":"The family Calloporidae occupies a basal positionin the classification of the Cheilostomata, the relatively simple calloporidmorphology seeming to be the basic pattern for the adaptive radiation ofcheilostome bryozoans. The ovicells of the Calloporidae may be formed by thematernal zooid or the distal zooid, which can be an autozooid, a vicariousavicularium or a kenozooid. Examination of new calloporid material from thesouthern Patagonian shelf revealed the existence of two new species and a newgenus. Kenoaplousina fissurata gen. nov., sp. nov. ischaracterized by its distinctive kenozooidal ooecium. The new genus is alsodeemed to include Aplousina grandipora Moyano, an allied species fromthe Chilean con-tinental slope. Alderina simplicissima sp. nov.,is also described from the Magellanic region.","palabras_clave":"BRYOZOAN|KENOZOOID|OVICELL STRUCTURE|TAXONOMY|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62730,"idioma_id":4,"titulo":"Delimiting species boundaries within the Bothriochloa saccharoides complex (Poaceae) through morphometric analysis","resumen":"The Bothriochloa saccharoides complex is one of the most interesting groups within the genus Bothriochloa (Poaceae).   The plants inhabit grasslands of tropical and subtropical regions from the Americas. Principal components analysis(PCA) and discriminant analysis (DA) were employed to evaluate the morphological variation among 60 herbarium specimens tentatively identified as B. imperatoides, B. laguroides, B. longipaniculata, B. saccharoides and B. torreyana.   Twenty-three morphological characters were included in the analysis in order to clarify problematic species boundaries.   Chemical data was incorporated to improve the resolution on delimitation of the species complex. Taxa were delimited according to the observed clustering of specimens in the PCA plots and discriminant analysis, and diagnostic characters were identified. The results showed that five taxa could be distinguished on the basis of morphological characters and   chemical data. Two new subspecies are described: B. saccharoides subsp. americana and B. saccharoides subsp. australis. An identification key and a taxonomic synopsis are provided.","palabras_clave":"American grasses|Gramineae|geographical distribution|multivariate analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62731,"idioma_id":4,"titulo":"Microspectrophotometric quantitation of immunohistochemical reactions by image analysis","resumen":"Immunohistochemistry is a technique by immunological reactions to identify proteins in different biological samples. As a result leave a product in histological preparations, visible under a microscope when using a color reaction. This is an indicator of the presence of the specific molecule and cellular metabolic activity represents a more or less specific. In the literature have used different methods to estimate the intensity of this reaction trying to get a reproducible value. With our microarray methodology (Cabrini RL et al.). We performed a spectral curve for the final product of the reaction of benzidine using as reaction control of a case inmunohistochemistry HER2 positive breast adenocarcinoma. Images were obtained from a single field of the preparation in a Zeiss MPM 800 with an objective x 40\/0, 75 different light wavelengths. The maximum absorption was 0.55 optical density at 530nm per pixel. The measurement was performed in 10 cases of carcinomas STA1. The methodology presented in this study showed different values at predetermined fixed areas.","palabras_clave":"Microspectrophotometric|Quantitation |Immunohistochemical |Image analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62732,"idioma_id":4,"titulo":"Modelling and uncertainties characterization for robust control","resumen":"In this work, multi-input multi-output (MIMO) process identification is studied, where the model identification is dedicated to the control design goal. An ad hoc identification procedure is presented which allows estimating not only a nominal parametric process model, but also a bound of the model uncertainty (i.e. modelling errors). The model structure is defined in a way that the identified nominal model and the uncertainties can readily be used for the analysis and design of a robust control system by means of many of the techniques available in the literature. Simulation examples are given to illustrate the method.","palabras_clave":"NONLINEAR IDENTIFICATION|UNCERTAINTY CHARACTERIZATION|ROBUST CONTROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62733,"idioma_id":4,"titulo":"Helicobacter pylori heterogeneity in patients with gastritis and peptic ulcer disease","resumen":"            Genetic diversification allows Helicobacter pylori to   persist during chronic colonization\/infection. We investigated the   intra-host variation of several markers that suggested microevolution in   patients with chonic gastritis (CG) and peptic ulcer disease (PUD).   One-hundred twenty-six isolates recovered from 14 patients with CG and   13 patients with PUD were analysed. cag pathogenicity island (cagPAI),   oipA, vacA, bab gene status and the presence of jhp0926, jhp0945,   jhp0947, jhp0949 and jhp0940 genes from the genomic Plasticity Zone (PZ)   were taken into accout to investigate intra-host variation.   lspA-glmM-RFLP was performed to identify mixed infections. Only one   patient was colonised\/infected by two ancestrally unrelated strains.   Among the 126 isolates, a significant association among cagPAI   genotypes, oipA status and vacA alleles was indicated. Complete cagPAI,   oipA 'on', and vacA s1-m1 variants were significantly found in patients   with PUD, without intra-host variations. Isolates from 7\/14 patients   with CG lacked babA in all chromosomal loci. In contrast, isolates from   all or several biopsies of PUD patients carried babA, but in one patient   only, the isolates showed positive Lewis b (Leb) binding assay.   Considering cagPAI, vacA, oipA, bab genotypes, intra-host variation was   also significantly higher in patients with CG. Conversely, a similarly   high intra-host variation in almost PZ genes was observed in isolates   from patients with CG and PUD. In conclusion, the lowest intra-host   variation in cagPAI, oipA, vacA, and bab genes found in patients with   PUD suggests the selection of a particular variant along the   bacteria-host environment interplay during ulceration development.   However, the predominance of this variant may be a refletion of the   multifactorial etiology of the disease rather than the cause, as it was   also found in patients with CG. The intra-host variation in PZ genes may   predict that this genomic region and the other markers of   microevolution studied evolve under diverse pressure(s).            Copyright © 2013 Elsevier B.V. All rights reserved.","palabras_clave":"HELICOBACTER  PYLORI|MICROEVOLUCION|GASTRITIS|ULCERA PEPTICAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62734,"idioma_id":4,"titulo":"Flavonols protect Arabidopsis plants against UV-B deleterious effects","resumen":"Flavonols are synthesized by flavonol synthase (FLS) enzymes (Martens et al., 2010). These compounds absorb UV-B light in the 280\u2013320 nm region, and their concentration increases in plants exposed to environmental abiotic and biotic stresses, including UV-B; consequently, flavonols are thought to act as UV-B filters (Agati et al., 2011). It has been also suggested that these metabolites function as reactive oxygen species (ROS) scavengers, as they contain an OH- group in the 3-position of the flavonoid skeleton, which allows them to chelate metals, inhibiting the formation of free radicals and ROS accumulation, once formed (Agati et al., 2009). For these reasons, it has been suggested that flavonols play uncharacterized roles in UV responses (Verdan et al., 2011). Nevertheless, despite the fact that the role of flavonols in UV-B protection has been inferred; the protection conferred by flavonols on the target sites of UV-B-damage has not been directly proven in planta. Recently, we demonstrated that maize FLS1 (ZmFLS1) complements the flavonol deficiency of the Arabidopsis fls1 mutant and decreases its high level of anthocyanins, characteristic of this mutant plant (Falcone Ferreyra et al., 2010). In order to demonstrate that flavonols protect plants against UV-B damage, we generated Arabidopsis transgenic plants overexpressing the maize FLS1 cDNA (35S:ZmFLS1), and evaluated different responses of these transgenic plants against UV-B damage.","palabras_clave":"FLAVONOLS|UV-B"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62735,"idioma_id":4,"titulo":"Deforestation and Reforestation of Latin America and the Caribbean (2001?2010)","resumen":"Forest cover change directly affects biodiversity, the global carbon budget, and ecosystem function. Within Latin American and the  Caribbean region (LAC), many studies have documented extensive deforestation, but there are also many local studies reporting forest  recovery. These contrasting dynamics have been largely attributed to demographic and socio-economic change. For example, local population  change due to migration can stimulate forest recovery, while the increasing global demand for food can drive agriculture expansion.  However, as no analysis has simultaneously evaluated deforestation and reforestation from the municipal to continental scale, we  lack a comprehensive assessment of the spatial distribution of these processes. We overcame this limitation by producing wall-to-wall,  annual maps of change in woody vegetation and other land-cover classes between 2001 and 2010 for each of the 16,050 municipalities  in LAC, and we used nonparametric Random Forest regression analyses to determine which environmental or population variables best  explained the variation in woody vegetation change. Woody vegetation change was dominated by deforestation (541,835 km2), particularly  in the moist forest, dry forest, and savannas\/shrublands biomes in South America. Extensive areas also recovered woody vegetation  (+362,430 km2), particularly in regions too dry or too steep for modern agriculture. Deforestation in moist forests tended to occur  in lowland areas with low population density, but woody cover change was not related to municipality-scale population change. These  results emphasize the importance of quantitating deforestation and reforestation at multiple spatial scales and linking these changes with  global drivers such as the global demand for food.","palabras_clave":"Deforestation|Reforestation|Latin America|Land use change"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62736,"idioma_id":4,"titulo":"Temporal and spatial patterns of macroinvertebrates associated with small and medium-size free-floating plants","resumen":"Macrophytes play an important role in structuring communities in aquatic ecosystems due to their influence on ecological processes and attributes of biological aquatic assemblages. Freshwater macroinvertebrates comprise a functionally and taxonomically diverse group in shallow lakes, which serve as food for fish, amphibians, and water birds, and are involved in the breakdown of organic matter and nutrients. Here, we investigated macroinvertebrate assemblages associated with small and medium-sized free-floating plants (FFP) by describing their structure, analyzing functional aspects (considering functional feeding groups and habits), and determining how much of the variation in fauna composition is explained by environmental factors, mainly FFP. Differences in structure, functional feeding groups and habits of macroinvertebrate assemblages were associated with different compositions and percentages of cover of FFP. Gradients of richness and diversity of macroinvertebrates were positively related to the complexity of FFP mats, which was associated with the structure of roots and leaves.","palabras_clave":"WETLANS|DIVERSITY|COMMUNITY STRUCTURE|HABITAT COMPLEXITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62737,"idioma_id":4,"titulo":"The new Wolf-Rayet binary system WR62a","resumen":"Context. A significant number of the Wolf-Rayet stars seem to be binary or multiple systems, but the nature of many of them is still unknown. Dedicated monitoring of WR stars favours the discovery of new systems. Aims: We explore the possibility that WR62a is a binary system. Methods: We analysed the spectra of WR62a, obtained between 2002 and 2010, to look for radial-velocity and spectral variations that would suggest there is a binary component. We searched for periodicities in the measured radial velocities and determined orbital solutions. A period search was also performed on the 'All-Sky Automated Survey' photometry. Results: We find that WR62a is a double-lined spectroscopic binary with a WN5 primary star and an O 5.5-6 type secondary component in orbit with a period of 9.1447 d. The minimum masses range between 21 and 23 Msun for the WN star and between 39 and 42 Msun for the O-type star, thus indicating that the WN star is less massive than the O-type component. We detect a phase shift in the radial-velocity curve of the He II 4686 emission line relative to the other emission line curves. The equivalent width of this emission line shows a minimum value when the WN star passes in front of the system. The analysis of the ASAS photometry confirms the spectroscopic periodicity, presenting a minimum at the same phase","palabras_clave":"binaries: spectroscopic|stars: individual: SMSNPL11 (=WR62a)|stars: Wolf-Rayet, stars: fundamental parameters"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62738,"idioma_id":4,"titulo":"Worldwide Phylogenetic Relationship of Avian Poxviruses","resumen":"Poxvirus infections have been found in 230 species of wild and domestic birds worldwide in both terrestrial and marine environments. This ubiquity raises the question of how infection has been transmitted and globally dispersed. We present a comprehensive global phylogeny of 111 novel poxvirus isolates in addition to all available sequences from GenBank. Phylogenetic analysis of the Avipoxvirus genus has traditionally relied on one gene region (4b core protein). In this study we expanded the analyses to include a second locus (DNA polymerase gene), allowing for a more robust phylogenetic framework, finer genetic resolution within specific groups, and the detection of potential recombination. Our phylogenetic results reveal several major features of avipoxvirus evolution and ecology and propose an updated avipoxvirus taxonomy, including three novel subclades. The characterization of poxviruses from 57 species of birds in this study extends the current knowledge of their host range and provides the first evidence of the phylogenetic effect of genetic recombination of avipoxviruses. The repeated occurrence of avian family or order-specific grouping within certain clades (e.g., starling poxvirus, falcon poxvirus, raptor poxvirus, etc.) indicates a marked role of host adaptation, while the sharing of poxvirus species within prey-predator systems emphasizes the capacity for cross-species infection and limited host adaptation. Our study provides a broad and comprehensive phylogenetic analysis of the Avipoxvirus genus, an ecologically and environmentally important viral group, to formulate a genome sequencing strategy that will clarify avipoxvirus taxonomy.","palabras_clave":"Poxviruses|Avian|Phylogeny|Worldwide"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62739,"idioma_id":4,"titulo":"Targeting galectin-1 overcomes breast cancer-associated immunosuppression and prevents metastatic disease","resumen":"Galectin-1 (Gal1), an evolutionarily conserved glycan-binding protein, contributes to the creation of an immunosuppressed microenvironment at sites of tumor growth. In spite of considerable progress in elucidating its role in tumor-immune escape, the mechanisms underlying the inhibitory functions of Gal1 remain obscure. Here, we investigated the contribution of tumor Gal1 to tumor growth, metastasis, and immunosuppression in breast cancer. We found that the frequency of Gal1+ cells in human breast cancer biopsies correlated positively with tumor grade, while specimens from patients with benign hyperplasia showed negative or limited Gal1 staining. To examine the pathophysiologic relevance of Gal1 in breast cancer, we used the metastatic mouse mammary tumor 4T1, which expresses and secretes substantial amounts of Gal1. Silencing Gal1 expression in thismodel induced a marked reduction in both tumor growth and the number of lung metastases. This effect was abrogated when mice were inoculated with wild-type 4T1 tumor cells in their contralateral flank, suggesting involvement of a systemic modulation of the immune response. Gal1 attenuation in 4T1 cells also reduced the frequency of CD4 +CD25+ Foxp3+ regulatory T (Treg) cells within the tumor, draining lymph nodes, spleen, and lung metastases. Further, it abrogated the immunosuppressive function of Treg cells and selectively lowered the expression of the T-cell regulatory molecule LAT (linker for activation of T cells) on these cells, disarming their suppressive activity. Taken together, our results offer a preclinical proof of concept that therapeutic targeting of Gal1 can overcome breast cancer-associated immunosuppression and can prevent metastatic disease. © 2012 American Association for Cancer Research.","palabras_clave":"Cancer Immunity|Breast Cancer|T regulatory cells|galectin-1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62740,"idioma_id":4,"titulo":"Beyond ?land sparing versus land sharing?: environmental heterogeneity, globalization and the balance between agricultural production and nature conservation","resumen":"By addressing the trade-offs between food production and  biodiversity conservation at landscape and ecoregion scales,  the land sparing\/sharing debate has made a significant  contribution to land use science. However, as global population  and food consumption grow, and urbanization and transnational trade intensify, land use trade-offs need to be analyzed at broader scales. These analyses should specifically consider the role of environmental heterogeneity on biodiversity distribution and agricultural suitability, the costs and benefits transferred far away from the focal land use,  institutional and economic factors influencing stability and  resilience, technology-related factors as mediators of  agriculture suitability, and bundles of different environmental  services. In addition, land use strategies to balance agriculture  and biodiversity conservation must consider local socioeconomic constraints and trade-offs.","palabras_clave":"land sparing|land sharing|land use change|jevons paradox"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62741,"idioma_id":4,"titulo":"Hydrolyzed galactomannan-modified nanoparticles and flower-like polymeric micelles for the active targeting of rifampicin to macrophages","resumen":"Inhalable nanocarriers that are uptaken by macrophages represent an appealing approach for the targeting of antibiotics to the tuberculosis reservoir. In the present work, we report on the development of rifampicin (RIF)-loaded nanoparticles and flower-like polymeric micelles surface-modified with hydrolyzed galatomannan (GalM-h), a polysaccharide of mannose and galactose, two sugars that are recognized by lectin-like receptors. Initially, pure or GalM-h-associated chitosan nanoparticles (NPs) were produced by ionotropic gelation. Despite the composition, NPs displayed positive zeta potential values between +18.0 and +24.5 mV and a size ranging between 263 and 340 nm. In addition, RIF payloads were approximately 1.0% w\/w. To increase the encapsulation efficiency, a more complex nanocarrier based on poly(epsilon-caprolactone)-b-poly(ethylene-glycol)-b-poly(epsilon-caprolactone) flower-like polymeric micelles (PMs) coated with chitosan or GalM-h\/chitosan were engineered. These polymeric micelles displayed a bimodal size distribution with a positive zeta potential between +6.7 and +8.1 mV. More importantly, the drug encapsulation capacity was increased 12.9-fold with respect to the NPs. An agglutination assay with concanavalin A confirmed the presence of GalM-h on the surface. Qualitative uptake studies by fluorescence microscopy revealed that GalM-h-modified systems were taken-up by RAW 264.7 murine macrophages. Finally, the intracellular\/cell associated levels of RIF following the incubation of cells with free or encapsulated drug indicated that while chitosan hinders the uptake, GalM-h leads to a significant increase of the intracellular concentration.","palabras_clave":"TUBERCULOSIS|CHITOSAN NANOPARTICLES|FLOWER-LIKE POLYMERIC MICELLES|HYDROLYZED GALACTOMANNAN|RIFAMPICIN|ACTIVE DRUG TARGETING TO MACROPHAGES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62742,"idioma_id":4,"titulo":"Selection of controlled variables: A novel perspective based on the singular energy of weighted graphs","resumen":"The optimal selection of controlled variables is a well-known plant-wide control subproblem. In this paper, a novel approach based on spectral graph theory is proposed. This strategy is useful from both graphical and mathematical point of views. It is shown here that if the closed-loop process is represented by a specific weighted graph, deviations in plant variables are bounded by the graph singular energy. Moreover, this graph-based methodology supports the fast interpretation of the magnitude and direction of influences between process variables at steady state. The suggested spectral approach is compared with the recently proposed minimum square deviation (MSD) methodology in detail. Indeed, both strategies have strong structural and behavioral resemblances, i.e. reducing specific deviations and improving the conditions of the subprocess to be controlled. The introduced graph representation is tested in the Shell oil fractionator process, giving a complete set of evaluations and results.","palabras_clave":"PLANT-WIDE CONTROL|WEIGHTED GRAPHS ENERGY|SINGULAR ENERGY|SELECTION OF CONTROLLED VARIABLES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62743,"idioma_id":4,"titulo":"Land Change in the Greater Antilles between 2001 and 2010","resumen":"Land change in the Greater Antilles differs markedly among countries because of varying socioeconomic histories and global influences. We assessed land change between 2001 and 2010 in municipalities (second administrative units) of Cuba, Dominican Republic, Haiti, Jamaica, and Puerto Rico. Our analysis used annual land-use\/land-cover maps derived from MODIS satellite imagery to model linear change in woody vegetation,  mixed-woody\/plantations and agriculture\/herbaceous vegetation. Using this approach, we focused on municipalities with significant change (p &#8804; 0.05). Between 2001 and 2010, the Greater Antilles gained 801 km2 of woody vegetation. This increase was mainly due to the return of woody vegetation in Cuba, and smaller increases in Puerto Rico and the  Dominican Republic. Despite relatively similar environments, the factors associated with these changes varied greatly between countries. In Puerto Rico, Dominican Republic, and Jamaica, agriculture declined while mixed-woody vegetation increased, mostly in montane regions. In contrast, Cuba experienced an extensive decline in sugarcane plantations, which  resulted in the spread of an invasive woody shrub species and the increase in woody vegetation in areas of high agricultural value. In Haiti, the growing population, fuelwood consumption, and increase in agriculture contributed to woody vegetation loss; however,woody vegetation loss was accompanied with a significant increase in the mixed woody and plantations class. Most regional analyses often treated the Greater Antilles as a homogeneous unit; our results suggest that historical and socio-economic differences among countries are crucial for understanding the variation in present day land change dynamics.","palabras_clave":"Caribe|Land use change|forest transition|Cuba"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62744,"idioma_id":4,"titulo":"Surface study of films formed on copper and brass at open circuit potential","resumen":"    The corrosion resistance of Cu-Zn alloys strongly  depends on the quality of the protective passive film. This study focuses on  the influence of Zn on the composition of oxide films on copper and brass  (Cu77Zn21Al2) in borax 0.1 mol L-1 (pH 9.2) solution, where the  solubility of copper oxides is minimal. The effect of the presence of chloride  ions at low concentration (0.01 mol L-1) in the electrolyte was also  evaluated. Both conditions were studied using a set of different  electrochemical, optical and surface techniques such as cyclic voltammetry,  differential reflectance, X-ray photoelectron spectroscopy and Raman  spectroscopy. A duplex Cu2O\/CuO layer forms on copper at potentials  positive to the open circuit potential (OCP), while in the case of brass, zinc  compounds are also incorporated to the surface film. It also became evident  that a surface film can be formed on these materials even at potentials  negative to the OCP. Zn(II) species are the main constituents of the films  growing on brass, while copper oxides are incorporated to the surface film when  approaching the OCP. The presence of chloride ions at low concentrations  contributes to the dissolution of the oxo-hydroxides formed during the early  stages of the ageing process at open circuit potential. Also, copper  chloro-compounds are formed, as shown by Raman spectroscopy for both copper and  brass electrodes.  ","palabras_clave":"Copper alloys | Surface films |Chloride ions |xps"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62745,"idioma_id":4,"titulo":"Phosphate ions as inhibiting agents for copper corrosion in chlorinated tap water","resumen":"PO43- ions as corrosion inhibitor were investigated on copper in tap water in the presence of NaClO. The inhibitor was evaluated by electrochemical techniques and weight loss tests. Raman spectroscopy and X-ray photoelectron spectroscopy were used to study the passive layer. In inhibited tap water, the passive layer is thick and compact if NaClO is present. Weight-loss tests showed the inhibition of uniform dissolution and no pitting attack. When adding NaClO, Cu3(PO4)2 is incorporated to the passive film. Thus, phosphate ions are effective as inhibitors for copper in tap water, even when using high dosages of biocides.","palabras_clave":"surfaces|eloectrochemical techniques|Raman spectroscopy|XPS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62746,"idioma_id":4,"titulo":"Phosphate ions as corrosion inhibitors for reinforcement steel in chloride-rich environments","resumen":"Phosphate ions were evaluated as corrosion inhibitors in solutions that simulate the composition of the pores in concrete contaminated with chloride ions. Cyclic voltammograms and potentiodynamic polarization tests were complemented with micro-Raman spectroscopy and impedance spectroscopy to follow the performance of this inhibitor. Long term performance involved weight loss evaluation. Chloride contamination increases the accumulation of corrosion products on the metallic surface and promotes pitting corrosion. In contrast, pitting is inhibited when phosphate ions are incorporated in a 1:1 phosphate to chloride molar ratio, even after a 90 days exposure. Micro Raman spectra clearly show the incorporation of phosphates to the passive film. Impedance  spectroscopy results can be interpreted assuming a duplex surface film formed in the presence of phosphates. In the conditions of this investigation, phosphate ions behave as mixed-type corrosion inhibitors, protecting steel against corrosion in chloride-contaminated  environments.","palabras_clave":"phosphate|inhibitors|chloride|steel"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62747,"idioma_id":4,"titulo":"Scalable design of an IMS cross-flow micro-generator\/ion detector","resumen":"Ion-mobility spectrometry (IMS) is an analytical technique used to separate and identifyionized gas molecules based on their mobility in a carrier buffer gas. Such methods come in alarge variety of versions that currently allow ion identification at and above the millimeterscale. Here, we present a design for a cross-flow IMS method able to generate and detect ionsat the sub-millimeter scale. We propose a novel ion focusing strategy and test it in a prototypedevice using nitrogen as a sample gas, and also with simulations using four different samplegases. By introducing an original lobular ion generation localized to a few ten of microns andsubstantially simplifying the design, our device is able to keep constant laminar flowconditions for high flow rates. In this way, it avoids the turbulences in the gas flow, whichwould occur in other ion-focusing cross-flow methods limiting their performance at thesub-millimeter scale. Scalability of the proposed design can contribute to improve theresolving power and resolution of currently available cross-flow methods.","palabras_clave":"ION-MOBILITY SPECTROMETRY|CROSS FLOW|MICRO-GENERATOR\/ION|SCALABLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62748,"idioma_id":5,"titulo":"PRIMERA RED SUDAMERICANA DE BIOMEDICINA. INVESTIGACIÓN, EDUCACIÓN Y BIOTECNOLOGÍA APLICADAS A LA SALUD","resumen":"Se da a conocer la creación del primer programa de integración regional de una red de Institutos  de Investigación en Biomedicina pertenecientes a países miembros del MERCOSUR. Se analizan  las bases que dieron sustento a su creación y sus objetivos en el mediano y largo plazo. Además, se estima el  potencial de los resultados de este programa en los campos de la investigación médica aplicada, educación y  biotecnología.","palabras_clave":"Mercosur|biomedicina|biotecnología"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62749,"idioma_id":4,"titulo":"Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS","resumen":"Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71KS was not affected by the presence of alpha-amylase, catalase and remained stable in a wide range of pH and after treatment with Triton X-100, Triton X-114, Tween 20, Tween 80, NaCl, SDS, urea and EDTA. This bacteriocin also remained active after being heated at 100 °C for 2 h and even after 20 min at 121 °C; however, it was inactivated by proteolitic enzymes. Production of bacteriocin ST71KS reached 6400 AU\/mL during stationary growth phase of Lb. plantarum cultivated in MRS at 30 °C and 37 °C. Bacteriocin ST71KS displayed a bactericidal effect against Listeria monocytogenes strains 603 and 607 and did not adsorb to the producer cells. Lb. plantarum ST71KS harbors two bacteriocin genes with homology to plantaricin S and pediocin PA-1. These characteristics indicate that bacteriocin ST71KS is a class IIa bacteriocin. The peptide presented no toxic effect when tested in vitro with kidney Vero cells, indicating safe technological application to control L. monocytogenes in foods.","palabras_clave":"LACTOBACILLUS PLANTARUM|LISTERIA MONOCYTOGENES|BACTERIOCIN|ANTIMICROBIAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62750,"idioma_id":4,"titulo":"Development of a high folate concentration yogurt naturally bio-enriched using selected lactic acid bacteria","resumen":"Folate concentrations in yogurts vary widely and are normally considered low due to an inadequate selection of starter cultures and fermentation conditions. In this study, folate producing Lactobacillus delbrueckii subsp. bulgaricus (3 strains) and Streptococcus thermophilus (2 strains) were combined and used to elaborate 15 different yogurts. Samples were taken at different time points during the fermentation process and folate concentrations were determined. The yogurt elaborated with strains CRL871+CRL803+CRL415 and incubated at 42 ºC had significantly higher folate concentrations (reaching 180 ± 10 µg\/L) which implies almost a 250 % increase in respect to non-fermented milk, and about 125 % compared to commercial yogurts. No variations in folate concentration were observed during 28 days of storage at 4 ºC. This is the first report of a yogurt naturally bio-enriched in folate using native folate producing starter cultures.","palabras_clave":"FOLATES|VITAMIN ENRICHMENT|YOGURT|LACTIC ACID BACTERIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62751,"idioma_id":4,"titulo":"Induction of hepatic multidrug resistance associated protein 3 by ethynylestradiol is independent of cholestasis and mediated by estrogen receptor","resumen":"Multidrug resistance\u2013associated protein 3 (Mrp3; Abcc3) expression and   activity are up-regulated in rat liver after in vivo                       repeated administration of ethynylestradiol (EE), a   cholestatic synthetic estrogen, whereas multidrug resistance-associated                       protein 2 (Mrp2) is down-regulated. This study was   undertaken to determine whether Mrp3 induction results from a direct   effect                       of EE, independent of accumulation of any   endogenous common Mrp2\/Mrp3 substrates resulting from cholestasis and   the potential                       mediation of estrogen receptor (ER). In in vivo   studies, male rats were given a single, noncholestatic dose of EE (5   mg\/kg                       s.c.), and basal bile flow and the biliary   excretion rate of bile salts and glutathione were measured 5 hours   later. This                       treatment increased Mrp3 mRNA by 4-fold, detected   by real-time polymerase chain reaction, despite the absence of   cholestasis.                       Primary culture of rat hepatocytes incubated with   EE (1\u201310 µM) for 5 hours exhibited a 3-fold increase in Mrp3 mRNA (10 µM),   consistent with in vivo findings. The increase in Mrp3 mRNA by EE was   prevented by actinomycin D, indicating transcriptional                       regulation. When hepatocytes were incubated with an   ER antagonist   [7&#945;,17&#946;-[9-[(4,4,5,5,5-Pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol                       (ICI182\/780), 1 µM], in addition to EE, induction of Mrp3 mRNA was abolished, implicating ER as a key mediator. EE induced an increase in ER-&#945;   phosphorylation at 30 minutes and expression of c-Jun, a well-known ER   target gene, at 60 minutes, as detected by Western                       blotting of nuclear extracts. These increases were   prevented by ICI182\/780. In summary, EE increased the expression of   hepatic                       Mrp3 transcriptionally and independently of any   cholestatic manifestation and required participation of an ER, most   likely                       ER-&#945;, through its phosphorylation.","palabras_clave":"hepatocytes|etinilestradiol|Mrp3|estrogen receptor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62752,"idioma_id":4,"titulo":"The allosteric modulation of thyroxine-binding globulin affinity is entropy driven","resumen":"Background: Thyroxine-binding globulin (TBG) is a non-inhibitory member of the serpin family of proteinswhose main structural element is the reactive center loop (RCL), that, upon cleavage by proteases, is insertedinto the protein core adopting a â-strand conformation (stressed to relaxed transition, S-to-R). After S-to-Rtransition thyroxine (T4) affinity decreases. However, crystallographic studies in the presence or absenceof the hormone in different states are unable to show significant differences in the structure and interactionsof the binding site. Experimental results also suggest the existence of several S states (differing in the numberof inserted RCL residues), associated with a differential affinity.Thyroxine-binding globulin (TBG) is a non-inhibitory member of the serpin family of proteinswhose main structural element is the reactive center loop (RCL), that, upon cleavage by proteases, is insertedinto the protein core adopting a â-strand conformation (stressed to relaxed transition, S-to-R). After S-to-Rtransition thyroxine (T4) affinity decreases. However, crystallographic studies in the presence or absenceof the hormone in different states are unable to show significant differences in the structure and interactionsof the binding site. Experimental results also suggest the existence of several S states (differing in the numberof inserted RCL residues), associated with a differential affinity.â-strand conformation (stressed to relaxed transition, S-to-R). After S-to-Rtransition thyroxine (T4) affinity decreases. However, crystallographic studies in the presence or absenceof the hormone in different states are unable to show significant differences in the structure and interactionsof the binding site. Experimental results also suggest the existence of several S states (differing in the numberof inserted RCL residues), associated with a differential affinity.finity decreases. However, crystallographic studies in the presence or absenceof the hormone in different states are unable to show significant differences in the structure and interactionsof the binding site. Experimental results also suggest the existence of several S states (differing in the numberof inserted RCL residues), associated with a differential affinity.ficant differences in the structure and interactionsof the binding site. Experimental results also suggest the existence of several S states (differing in the numberof inserted RCL residues), associated with a differential affinity.finity.Methods: To shed light into the molecular basis that regulates T4 affinity according to the degree of RCL insertionin TBG,weperformed extendedmolecular dynamics simulations combinedwith several thermodynamic analysisof the T4 binding to TBG in three different S states, and in the R state.To shed light into the molecular basis that regulates T4 affinity according to the degree of RCL insertionin TBG,weperformed extendedmolecular dynamics simulations combinedwith several thermodynamic analysisof the T4 binding to TBG in three different S states, and in the R state.Results: Our results show that, despite T4 binding in the protein by similar interactions in all states, a good correlationbetween the degree of RCL insertion and the binding affinity, driven by a change in TBG conformationalentropy, was observed.Our results show that, despite T4 binding in the protein by similar interactions in all states, a good correlationbetween the degree of RCL insertion and the binding affinity, driven by a change in TBG conformationalentropy, was observed.finity, driven by a change in TBG conformationalentropy, was observed.Conclusion: TBG allosteric regulation is entropy driven. The presence of multiple S states may allow moreefficient T4 release due to protease activity.TBG allosteric regulation is entropy driven. The presence of multiple S states may allow moreefficient T4 release due to protease activity.ficient T4 release due to protease activity.General significance: The presented results are clear examples of howcomputer simulationmethods can reveal thethermodynamic basis of allosteric effects, and provide a general framework for understanding serpin allostericaffinity regulation.© 2013 Elsevier B.V. All rights reservedficance: The presented results are clear examples of howcomputer simulationmethods can reveal thethermodynamic basis of allosteric effects, and provide a general framework for understanding serpin allostericaffinity regulation.© 2013 Elsevier B.V. All rights reservedfinity regulation.© 2013 Elsevier B.V. All rights reserved","palabras_clave":"SERPIN |THYROXIN-BINDING GLOBULIN|ALLOSTERY|CONFORMATIONAL ENTROPY|GENERALIZED BORN SURFACE ANALYSIS|MOLECULAR DYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62753,"idioma_id":4,"titulo":"Magnetic and Conducting Properties of Composites of Conducting  Polymers and Ferrite Nanoparticles","resumen":"          Composites of ferromagnetic CoFe2O4  nanoparticles and two conducting polymers (polyethylenedioxythiophene -PEDOT-  and polypyrrole -Ppy-) were prepared and characterized. Both synthesis were  performed by monomer polymerization in presence of a dispersion of the magnetic  nanoparticles, at different monomer:CoFe2O4 molar ratios.  For PPy-composites, both the coercive field and the applied field required to  reach the maximum magnetization decrease as the polymer content increases. For  PEDOT-composites, the remanence ratio increases as the polymer content  increases, indicating the presence of interactions related to the amount of  polymer present. Electrical conductivity measurements indicate that, for both  types of composites, a high polymer content gives rise to high electrical  conductivity. These results indicate that the composite properties can be  modulated by varying the polymer identity and the monomer:CoFe2O4  molar ratio.   ","palabras_clave":"CONDUCTING MATERIALS|FERRITES|MAGNETIC ANALYSIS|MAGNETIC NANOPARTICLES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62754,"idioma_id":4,"titulo":"Spatial Resolution in Micrometric Periodic Assemblies of Magnetotactic Bacteria and Magnetic Nanoparticles","resumen":"We developed a simple method for obtaining micro arrays of magnetic nanoparticles using audio tapes. We present spatial micro-arrangements of magnetotactic bacteria (Magnetospirillum gryphiswaldense), magnetite (Fe O ) and functionalized cobalt ferrite (CoFe O ) nanoparticles. Computer generated square audio waves of different frequencies (100 Hz?10 kHz) were recorded leading to magnetic patterns of different micrometer spatial wavelengths. Drops of aqueous suspensions were deposited on the tapes to control particle density, and bacteria and particles were trapped at locations where magnetic energy is minimized, as observed using conventional optical microscopy after the dispersium medium was evaporated. We discuss the spatial limits of the magnetic nanoparticles and the bacteria assemblies, concluding that cell walls of bacteria inhibit agglomeration and optimize spatial organization.","palabras_clave":"MAGNETIC NANOPARTICLES|MAGNETIC PARTICLE IMAGING|MAGNETIC TAPES|MAGNETITE|MICROASSEMBLY|MICROORGANISMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62755,"idioma_id":4,"titulo":"Cascading top-down effects on estuarine intertidal meiofaunal and algal assemblages","resumen":"Interactions between organisms areimportant determinants of species distributions and abundances. Due to thehighcomplexity of interactions between species in natural systems, the outcome of agiven interaction can affect others, finally modifying community composition. InSouth-Western Atlantic intertidal mudflats, the zonation of the burrowing crab Neohelice (Chasmagnathus) granulata and the intertidalsnail Heleobiaaustralis rarely overlaps, suggestingthat both speciesmight have negative interactions; and, given that both specieshave different foraging strategies, these negative interactions can have top-downimpacts on community composition. Zonation patterns of both species showed thatsnails are more abundant in areas without crab burrows, and field experiments revealed that snaildensity correlated with a reduction in crab density and that when crabs wereexcluded, snails were able to colonize those higher intertidal areas.Bioturbation and not competition seems to drive that pattern, given that crabshave no effects onmicroalgae, but negatively affect infaunal organisms such ascopepods, flagellates, naupllilarvae and snails. Conversely, snails negatively affect algal assemblages,specificallycyanobacteria, chlorophytes, and euglenophytes, although diatoms, the mostabundant group, was not modified. Our results showthat crab?snail competitiondisrupts snail herbivory upon microalgae by limiting the area over which algalconsumption occurs and highlight the complex web of interactions thatfrequently regulates community structure in natural systems.","palabras_clave":"CRABS|INTERACTIONS|ALGAL ASSEMBLAGE|MUDFLATS|SNAILS|TOP-DOWN EFFECTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62756,"idioma_id":4,"titulo":"Comparison of mating system parameters and genetic structure in three natural scenarios of Acacia visco (Leguminosae, Mimosoideae)","resumen":"Acacia visco is a native South American tree species that has been extensively used for ornamental purposes and in carpentry, bodywork and parquet due to the hardness and durability of its wood. Little is known about genetic diversity and mating system of A. visco. The main aims of this study were to (1) estimate outcrossing rates in natural Argentinean populations using AFLP markers, (2) test for any difference in mating patterns among a large a patchy and relict population, and (3) compare the mating system of A. visco with other Acacia species. The three primer pairs used in the AFLP analysis revealed a total of 569 variable loci. Most genetic variation was observed among individuals within families (61.2 %). The estimate of multilocus outcrossing rate (tm) was high (C0.971) in all populations. Average pairwise coancestry between progenies within families for each population ranged from 0.082 to 0.105 or from 0.125 to 0.136, depending on the method h was estimated. In the three populations studied, the progenies of open pollination were constituted mainly for half-sibs (94.3 %). This work shows a similar mating system in all populations of A. visco in spite of their size differences, hypothesizing that the entire species has a similar mating system of outcrossing preferential. Considering the results obtained here where a high percentage of individuals were half-sibs, sampling large numbers of pods from individual trees for ex situ conservation will result in a genetically diverse sample as a consequence of high outcrossing rates.","palabras_clave":"ACACIA VISCO|AFLP|MATING SYTEM PARAMETERS|GENETIC STRUCTURE|DIFFERENT NATURAL SCENARIOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62757,"idioma_id":4,"titulo":"NEW MICROSATELLITE LOCI FOR PROSOPIS ALBA AND P. CHILENSIS (FABACEAE)","resumen":"Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis.Methods and Results: Twelve markers that were successfully amplified showed polymorphism in P. alba and P. chilensis. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia , P. flexuosa , P. kuntzei , P. glandulosa , and P. pallida.Conclusions: These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.","palabras_clave":"FABACEAE|MICROSATELLITE|NEXT-GENERATION SEQUENCING DATA|PROSOPIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62758,"idioma_id":4,"titulo":"Spatial patterns of the Argentine hake Merluccius hubbsi and oceanographic processes in a semi-enclosed Patagonian ecosystem","resumen":"Time-series of fishing position, landings, satellite-derived sea surface temperature and chlorophyll a concentrations were used to relate the spatial-temporal distribution of the Argentine hake Merluccius hubbsi with seasonal oceanographic processes in San Matías Gulf. Also, the seasonal effect of fishing on the hake population structure was analysed. During summer the fleet was concentrated over the area of the frontal system, obtaining the best catch-per-unit-effort (CPUE) of hake in relatively deep waters. In autumn, the dispersion of the fleet due to a reduction in CPUE coincided with the dissipation of the front, suggesting that the distribution and shoaling of the Argentine hake is associated with seasonal thermal structures. In spring, the thermal structure of the waters and the Chlorophyll a blooms seem to modulate the timing of spawning of hake, which occurs mainly in October-November. In addition, the fleet captured a higher proportion of females in the gonadal recovery stage during warm months (November to April). While winter catches (May to October) consisted mainly of males, the intense summer fishing may result in a high impact on the female population. This information is relevant to design of spatial management tools intended to provide biological sustainability to the hake fishery.","palabras_clave":"Natural resources distribution|Tidal front|Trawl fishery|Satellite-derived data|Sustainable management"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62759,"idioma_id":4,"titulo":"SERS Active Surface in Two Steps, Patterning and Metallization","resumen":"Robust and reproducible metallized nano\/microstructured surfaces of polymeric surfaces have been successfully prepared by direct laser interference patterning (DLIP) of commercial polymeric films followed by sputtering of metallic thin films. The SERS spectra for 2-thioaniline adsorbed on a structured polycarbonate surfaces covered with a gold or platinum film showed a ca. three order of magnitude enhancement over a flat surface with the same metal film. The method here reported is suitable for mass production of substrates for SERS since large areas (several cm2) can be structured in ca. 1?5&#8201;s.","palabras_clave":"raman|ablacion laser|polimeros"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62760,"idioma_id":5,"titulo":"Coexistencia de instituciones de órdenes normativos contrapuestos en la Epístola a Filemón","resumen":"Esta revista se encuentra indizada en Latindex-directorio.                  Resumen:                  En la Epístola  a Filemón Pablo se detiene en el cambio de estatus de Onésimo dentro de la  comunidad cristiana y de la casa de Filemón: ya no es un simple esclavo sino un  miembro de la comunidad en el mismo nivel que Filemón y los demás cristianos.                   Onésimo es, para el derecho positivo, un esclavo, y  para la comunidad cristiana, un hijo más a partir de su bautismo. La rogatoria  a favor de Onésimo se asienta en la hermandad de los que siguen el evangelio: Pablo  no propone la abolición de la esclavitud pero le pide a Filemón que trate a su  esclavo como a su propio hermano.                  Este trabajo examina la contraposición que surge entre  el derecho divino y el derecho positivo en la Epístola a Filemón a partir de la conducta suplicada al receptor de  la misiva y de la figura del esclavo redimido en Cristo.","palabras_clave":"instituciones|órdenes normativos|Epístola a Fillemón|esclavitud|hermandad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62761,"idioma_id":4,"titulo":"Spreading of AbaR-type genomic islands in multidrug resistance Acinetobacter baumannii strains belonging to different clonal complexes.","resumen":"In order to determine the occurrence of AbaR-type genomic island in multidrug resistant Acinetobacter baumannii (MDRAb) strains circulating in Argentina, Uruguay, and Chile, we studied 51 MDRAb isolates recovered from several hospitals over 30&nbsp;years. AbaR-type genomic resistance islands were found in 36 MDRAb isolates since 1986 till now. MLST technique allowed us to identify the presence of four different Clonal Complexes (109, 104, 119, 113) among the positive AbaR-type island positive strains. This is the first description of AbaR-type islands in the CC104 and CC113 that are the most widespread Clonal Complexes in Argentina. In addition, PCR mapping exposed different arrays to those previously described, evidencing the plasticity of this island. Our results evidence a widespread distribution of the AbaR-type genomic islands along the time in the MDRAb population, including the epidemic global clone 1 (GC1) as well as different clonal complexes to those already described in the literature.","palabras_clave":"Genomic Islands|Acinetobacter baumannii|Integrons|Transposons"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62762,"idioma_id":4,"titulo":"Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I-III.","resumen":"As a way to contribute to the assessment of Acinetobacter baumannii clinical population structure, multilocus  sequence typing (MLST) was performed in a collection of 93 isolates from Buenos Aires (1983?  2012) and Rosario (2006?2009) hospitals. Sequence types (STs) were achieved by Bartual (B) and Institut  Pasteur (P) schemes. PFGE typing, antimicrobial susceptibility assays, and the amplification of the OXA  carbapenemase genes most prevalent in our region, were also performed.  e-Burst clustered the 25 STsB (15 novels) into 5 clonal complexes (CC) and 5 singletons, and grouped  the 18 STsP (12 novels) into 3 CC and 4 singletons. Bartual scheme divided the CC79P into two groups.  CC113B\/CC79P prevailed in Buenos Aires at least in 1992?2009, being responsible for epidemic and for  endemic infections and acquiring the XDR (extensively drug-resistant) pattern throughout the years.  While, CC119B\/CC79P was apparently present before the CC113B\/CC79Pdomain. CC103B\/CC15P was the  second most prevalent CC. Interestingly, CC110B\/ST25P apparently increased over the last years. Conversely,  CC109B\/CC1P (international clone I) predominated in Rosario, although the presence of  CC113B\/CC79P, CC103B\/CC15P and CC110B\/ST25P was observed. Nineteen novel STs clustered in CC79P,  CC15P, CC113B, CC109B and CC103B, suggesting their clonal expansion during persistence. PFGE typing  proved transmission of strains intra- and inter-hospitals in each city. Except for one, all the recent isolates  (2007?2012) harboured the blaOXA-23-like. All isolates were susceptible to colistin. Tigecycline MIC90 was  1 mg\/L and the rifampicin MIC > 512 mg\/l was found among isolates in three hospitals.  In conclusion, the international clone II (CC92B\/CC2P) was not found among our isolates. CC113B\/CC79P,  CC103B\/CC15P, and ST25P, suggested also as major components in the A. baumannii population together  with the international clone I, were present in Buenos Aires and Rosario with different prevalence rate.  Their recent isolates showed high distribution of the blaOXA-23-like as well as the XDR pattern.","palabras_clave":"Acinetobacter baumannii|MLST|clone"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62763,"idioma_id":4,"titulo":"Heavy metals concentrations in zooplankton and suspended particulate matter in a southwestern Atlantic temperate estuary (Argentina)","resumen":"The present work includes part of the first studies of metals concentrations in the zooplankton from a heavily industrialized estuary of Argentina, the Bahía Blanca estuary. Cd, Cu, and Pb concentrations inthe zooplankton (macro- and mesozooplankton) and the suspended particulate matter were measured at stations with different degree of pollution. Physicochemical variables and zooplankton composition and abundance were also analyzed. Thus, the aim of the present work was to analyze the spatial and temporal distribution of heavy metals in these two different fractions, and the possible relation among them due to their importance inthe biogeochemical cycles of marine environments.Samplings were carried out during a year, from March 2005 to April 2006, every 2 months, at stations located near chemical and petrochemical industries, stations far from these points and one station in an intermediate location. In the mesozooplankton, the mean concentrationsof Cd, Cu, and Pb were 3.63±1.46, 34.46±5.40, and 11.54±3.04 &#956;gg&#8722;1 dry weight (d.w.) respectively, while in the macrozooplankton, 3.20±2.28, 21.86±4.79, and 8.36±1.85 &#956;gg&#8722;1 d.w. On the other hand, particulate Cd, Cu, and Pb presented a mean concentration of 3.33±1.22, 12.75±2.67, and 12.53±3.20 &#956;gg&#8722;1 d.w., respectively. Metals? levels in both the SPM andzooplankton fluctuated throughout the study time and were relatively high in the particulate phase especially for Cu and Pb. Moreover, zooplankton accumulated important concentrations of the three metals. The sourcesof them are probably the discharges of the industries and domestic sewages located near the estuary.","palabras_clave":"Heavy metals|Estuarine Zooplankton|Suspended particulate matter|Pollution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62764,"idioma_id":4,"titulo":"Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams","resumen":"Abstract   Neospora caninum infection is a major cause of  abortion in cattle. The objectives of this study were to genetically  characterize the N. caninum NC-6 Argentina isolate  using a multilocus microsatellite analysis approach and to  study its biological behavior by experimental inoculations into  seronegative and seropositive pregnant cattle, evaluating the  humoral and cellular immune response elicited and the occurrence  of transplacental transmission and fetopathy. Pregnant  cows (65 days of gestation) seropositive and seronegative  to N. caninum were intravenously inoculated with tachyzoites  of the NC-6 Argentina N. caninum strain and slaughtered at  108±2 days of gestation. Serum samples were analyzed for N.  caninum antibodies by indirect fluorescent antibody test. The  cellular immune response was analyzed by detection of gamma  interferon (&#947;IFN) production in blood cells. Tissue samples  from dams, fetuses, and placental cotyledons were  processed by histopathological and immunohistochemical  techniques and examined for N. caninum DNA by PCR.  Positive DNA samples were further analyzed by multilocus  microsatellite typing for N. caninum. Inoculated animals had  significantly higher N. caninum antibody titers and &#947;IFN  production than control animals. One seropositive inoculated  cow aborted, one seronegative cow had a non-viable fetus,  and the remaining fetuses from the experimentally inoculated  dams had histopathologic lesions. The PCR was positive in  3\/4 fetuses from seronegative inoculated cows and in 2\/3  fetuses from seropositive inoculated cows. Multilocus microsatellite  analysis revealed that the N. caninum DNA present in  fetuses and placentas had an identical pattern to NC-6  Argentina strain. The NC-6 Argentina strain proved to be able  to cross the placenta and to induce","palabras_clave":"NEOSPORA CANINUM NC-6 ARGENTINA|FETOPATHY|EXPERIMENTALLY INOCULATION|PREGNANT DAMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62765,"idioma_id":4,"titulo":"Present and future global distributions of the marine Cyanobacteria Prochlorococcus and Synechococcus","resumen":"The Cyanobacteria Prochlorococcus and Synechococcus account for  a substantial fraction of marine primary production. Here, we present  quantitative niche models for these lineages that assess present  and future global abundances and distributions. These niche models  are the result of neural network, nonparametric, and parametric  analyses, and they rely on >35,000 discrete observations from all  major ocean regions. The models assess cell abundance based on  temperature and photosynthetically active radiation, but the individual  responses to these environmental variables differ for each  lineage. The models estimate global biogeographic patterns and  seasonal variability of cell abundance, with maxima in the warm  oligotrophic gyres of the Indian and the western Pacific Oceans  and minima at higher latitudes. The annual mean global abundances  of Prochlorococcus and Synechococcus are 2.9 ± 0.1 × 1027 and 7.0 ±  0.3 × 1026 cells, respectively. Using projections of sea surface temperature  as a result of increased concentration of greenhouse gases  at the end of the 21st century, our niche models projected increases  in cell numbers of 29% and 14% for Prochlorococcus and Synechococcus,  respectively. The changes are geographically uneven but  include an increase in area. Thus, our global niche models suggest  that oceanic microbial communitieswill experience complex changes  as a result of projected future climate conditions. Because of the high  abundances and contributions to primary production of Prochlorococcus  and Synechococcus, these changes may have large impacts on  ocean ecosystems and biogeochemical cycles.","palabras_clave":"climate change|marine cyanobacteria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62766,"idioma_id":4,"titulo":"Understanding the dynamics of an enclosed trawl demersal fishery in Patagonia (Argentina): A holistic approach combining multiple data sources","resumen":"Understanding the dynamics of a fishery is a key ingredient to successful fishery management. The objective of this study was to improve the understanding of the dynamics of the trawl demersal fishery that operates in San Matías Gulf (Patagonia, Argentina). This system offers an opportunity to explore different issues of fishery dynamics and management because it is a simple system with a few well-characterised  active vessels and a stock of Argentine hake (Merluccius hubbsi) that seems to have been well preserved since the beginning of the fishery in 1970. This study combined different sources of information to analyse  the seasonality of landings, the link between catch and landing profiles, and the association between sea surface temperature and the spatio-temporal variability in catch profiles. The monthly species composition  of the catch fell into three fishing seasons, each with a distinct landing profile. During warm months, M. hubbsi dominated the catch, and bottom trawl activity was concentrated in a thermal frontal zone.  In winter, the fishing activity was more dispersed over the surface of the gulf, and yields were minimal. From August to October, the landings increased rapidly due to the catch of Seriolella porosa, and the distribution of the fishing effort was partially concentrated in the northeastern area of the gulf. The seasonal pattern observed would be related to the resource availability and the commercial opportunities. These factors were significant in determining what, when, and where to fish in the trawl demersal fishery of San Matías Gulf.","palabras_clave":"fishery dynamics|trawl fishery|catch profile|Patagonia|fishery management"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62767,"idioma_id":4,"titulo":"Differential regulation of feeding rhythms through a multiple-photoreceptor system in an avian model of blindness","resumen":"All organisms have evolved photodetection systems to synchronize their physiology and behavior with the external light-dark (LD) cycles. In nonmammalian vertebrates, the retina, the pineal organ, and the deep brain can be photoreceptive. Inner retinal photoreceptors transmit photic information to the brain and regulate diverse nonvisual tasks. We previously reported that even after preventing extraretinal photoreception, blind GUCY1* chickens lacking functional visual photoreceptors could perceive light that modulates physiology and behavior. Here we investigated the contribution of different photoreceptive system components (retinal\/pineal and deep brain photoreceptors) to the photic entrainment of feeding rhythms. Wild-type (WT) and GUCY1* birds with head occlusion to avoid extraocular light detection synchronized their feeding rhythms to a LD cycle with light &gt;12 lux, whereas at lower intensities blind birds free-ran with a period of &gt;24 h. When released to constant light, both WT and blind chickens became arrhythmic; however, after head occlusion, GUCY1* birds free-ran with a 24.5-h period. In enucleated birds, brain illumination synchronized feeding rhythms, but in pinealectomized birds only responses to high-intensity light (¡Ý800 lux) were observed, revealing functional deep brain photoreceptors. In chickens, a multiple photoreceptive system, including retinal and extraretinal photoreceptors, differentially contributes to the synchronization of circadian feeding behavior","palabras_clave":"PHOTOTRANSDUCTION|INNER RETINA|PINEAL GLAND|CIRCADIAN RHYTHMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62768,"idioma_id":4,"titulo":"Diacylglycerol kinase epsilon in bovine and rat photoreceptor cells. Light-dependent distribution in photoreceptor cells","resumen":"        The present study  shows the selective light-dependent distribution of 1,2-diacylglycerol kinase  epsilon (DAGKe) in photoreceptor cells from bovine and albino rat  retina. Immunofluorescence microscopy in isolated rod outer segments from  bleached bovine retinas (BBROS) revealed a higher DAGKe signal than that found in rod outer segments from  dark-adapted bovine retinas (BDROS). The light-dependent outer segment  localization of DAGKe was also observed by immunohistochemistry in retinas from  albino rats. DAGK activity, measured in terms of phosphatidic acid formation  from a) [3H]DAG and ATP in the presence of EGTA and R59022, a type I  DAGK inhibitor, or b) [g-32P]ATP  and 1-stearoyl, 2-arachidonoylglycerol (SAG), was found to be significantly  higher in BBROS than in BDROS. Higher light-dependent DAGK activity (condition  b) was also found when ROS were isolated from dark-adapted rat retinas exposed  to light. Western blot analysis of isolated ROS proteins from bovine and rat  retinas confirmed that illumination increases DAGKe content in the outer segments of these two species. Light-dependent  DAGKe localization in the outer segment was not observed when  U73122, a phospholipase C inhibitor, was present prior to the exposure of rat  eye cups (in situ model) to light. Conversely,  no increased PA synthesis from [3H]DAG and ATP was observed in the  presence of neomycin prior to the exposure of bovine eyecups to light. Interestingly,  when BBROS were pre-phosphorylated with ATP in the presence of 1,2-dioctanoyl  sn-glycerol (diC8) or phorbol dibutirate (PDBu) as PKC activation conditions, higher  DAGK activity was observed than in dephosphorylated controls.      Taken together, our findings  suggest that the selective distribution of DAGKe in photoreceptor cells is a light-dependent mechanism  that promotes increased SAG removal and synthesis of 1-stearoyl, 2-arachidonoyl  phosphatidic acid in the sensorial portion of this cell, thus demonstrating a  novel mechanism of light-regulated DAGK activity in the photoreceptors of two  vertebrate species.    ","palabras_clave":"DIACYLGLYCEROL KINASE EPSILON|RETINA|ROD OUTER SEGMENT|LIGHT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62769,"idioma_id":4,"titulo":"THERAPEUTIC EFFECTS OF PROGESTERONE IN ANIMAL MODELS OF NEUROLOGICAL DISORDERS","resumen":"Substantial evidence supports that progesterone exerts many functions in the central and peripheral nervoussystem unrelated to its classical role in reproduction. In this review we first discussed progesterone effects followingbinding to the classical intracellular progesterone receptors A and B and several forms of membrane progesteronereceptors, the modulation of intracellular signalling cascades and the interaction of progesterone reduced metabolites withneurotransmitter receptors. We next described our results involving animal models of human neuropathologies toelucidate the protective roles of progesterone. We described: (a) the protective and promyelinating effects of progesteronein experimental spinal cord injury, (b) the progesterone protective effects exerted upon motoneurons in the degeneratingspinal cord of Wobbler mouse model of amyotropic lateral sclerosis; (c) the protective and anti-inflammatory effects ofprogesterone in the murine experimental autoimmune encephalomyelitis model of multiple sclerosis and after lysolecithindemyelination; (d) the progesterone prevention of nociception and neuropathic pain which follow spinal cord injury, and(e) the protective effect of progesterone in experimental ischemic stroke. Whenever available, the molecular mechanismsinvolved in these progesterone effects were examined. The multiplicity of progesterone beneficial effects has opened newvenues of research for neurological disorders. In this way, results obtained in animal models could provide the basis fornovel therapeutic strategies and pre-clinical studies.","palabras_clave":"PROGESTERONE|NEUROPROTECTION|STEROID RECEPTORS|ANIMAL MODELS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62770,"idioma_id":4,"titulo":"Development of a modified release hydrophilic matrix system of a plant extract based on co-spray-dried powders","resumen":"The aim of this paper was to develop a modified-release solid dosage form containing a plant extract, namely Hamamelis virginiana extract. To this purpose, a co-spray-dried powder consisting of Hamamelis virginiana extract, sodium carboxymethylcellulose (Na-CMC) and colloidal silicon dioxide (SiO2) was formulated, which showed good flow properties for direct compression and was useful as a modified-release hydrophilic matrix system. H. virginiana extract was selected as a model plant extract due to its extensive use in treatments for chronic varicose veins. Different ternary co-processed powders were obtained by modifying the viscosity grade and concentration of Na-CMC. For a given set of spray-drying operating conditions, the use of medium viscosity (MV) Na-CMC in the formula led to high process yields and satisfactory product moisture contents. Compacted powders were able to modulate the extract release in different media, being the polymer concentration the fundamental factor affecting the rate of drug release. The co-spray-dried powder with a plant extract solid residue:SiO2:Na-CMC MV ratio of 1:1:0.075 was the best formulation because of its good flowability, compactability, stability and release properties. It was found that the release mechanism was a combination of extract diffusion and matrix erosion. The results indicated that the co-spray-drying of adequately designed formulations is a good strategy to improve the functionality of medicinal plant extract powders.","palabras_clave":"Hamamelis virginiana|Sodium carboxymethylcellulose|Spray-drying|Matricial system"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62771,"idioma_id":4,"titulo":"Solving the over segmentation problem in applications of  Watershed Transform","resumen":"Background: The Watershed Transform consists of an image partitioning into its constitutive regions. This transform is easily adapted to be used in different types of images and it allows distinguishing complex objects. However, the implementation of the Watershed Transform for very complex images actually produces over-segmentation. In this paper we propose two algorithms to solve this over-segmentation problem. Methods: We define internal markers, by algorithms based on clustering and fuzzy logic in order to join the oversegmented regions with statistical features. To define the algorithm parameters and evaluate their performance, errors against images segmented manually were measured and ROC curves were determined.  Results: The results show that the proposed methods self-adapt to the different image objects characteristics. An  improvement of the accuracy is obtained.  Conclusions: This analysis will contribute in images segmentation where complexity of the objects is high.","palabras_clave":"WATERSHED|OVERSEGMENTATION|FUZZY|CLUSTERING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62772,"idioma_id":4,"titulo":"Cross-Desensitization and Cointernalization of H1 and H2 Histamine Receptors Reveal New Insights into Histamine Signal Integration","resumen":"G protein-coupled receptor signaling does not result from sequential activation of a linear pathway of proteins\/enzymes, but rather from complex interactions of multiple, branched signaling routes, i.e., signaling networks. In this work we present an exhaustive study of the cross-talk between H1 and H2 histamine receptors (H1R and H2R) in U937 cells and Chinese hamster ovary-transfected cells. By desensitization assays we demonstrated the existence of a crossdesensitization between both receptors independent of protein kinase A or C. H1R-agonist stimulation inhibited cell proliferation and induced apoptosis in U937 cells following treatment of 48 hours. H1R-induced antiproliferative and apoptotic response was inhibited by an H2R agonist suggesting that the cross-talk between both receptors modifies their function. Binding and confocal microscopy studies revealed cointernalization of both receptors upon treatment with the agonists. To evaluate potential heterodimerization of the receptors, sensitized emission fluorescence resonance energy transfer experiments were performed in human embryonic kidney 293T cells using H1R-cyan fluorescent protein and H2R-yellow fluorescent protein. To our knowledge these findings may represent the first demonstration of agonist-induced heterodimerization of the H1R and H2R. In addition, we also show that the inhibition of the internalization process did not prevent receptor crossdesensitization, which was mediated by G protein-coupled receptor kinase 2. Our study provides new insights into the complex signaling network mediated by histamine and further knowledge for the rational use of its ligands.","palabras_clave":"Histamine|Histamine receptors|Receptor desensitization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62773,"idioma_id":4,"titulo":"Implication of von Willebrand Factor as a Regulator of Tumor Cell Metastasis: Potential Perioperative Use of Desmopressin and Novel Peptide Analogs.","resumen":"Peptides such as DDAVP have a great potential as therapeutic agents due to their ease of rational design and target specificity, and cancer therapy constitutes an important field for peptide compounds.","palabras_clave":"VON WILLEBRAND FACTOR |METASTASIS|PEOPERATIVE USE|DESMOPRESSIN|ANALOGS OF DESMOPRESSIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62774,"idioma_id":4,"titulo":"The Impact of the Long-Distance Transport of a BEL1-Like Messenger RNA on Development","resumen":"BEL1- and KNOTTED1-type proteins are transcription factors from the three-amino-loop-extension superclass that interact in atandem complex to regulate the expression of target genes. In potato (Solanum tuberosum), StBEL5 and its Knox protein partnerregulate tuberization by targeting genes that control growth. RNA movement assays demonstrated that StBEL5 transcripts movethrough the phloem to stolon tips, the site of tuber induction. StBEL5 messenger RNA originates in the leaf, and its movement tostolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increasedgrowth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts.Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.Solanum tuberosum), StBEL5 and its Knox protein partnerregulate tuberization by targeting genes that control growth. RNA movement assays demonstrated that StBEL5 transcripts movethrough the phloem to stolon tips, the site of tuber induction. StBEL5 messenger RNA originates in the leaf, and its movement tostolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increasedgrowth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts.Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 transcripts movethrough the phloem to stolon tips, the site of tuber induction. StBEL5 messenger RNA originates in the leaf, and its movement tostolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increasedgrowth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts.Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 messenger RNA originates in the leaf, and its movement tostolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increasedgrowth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts.Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 RNA to roots correlated with increasedgrowth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts.Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts.Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark,promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor,binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motifin the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolontips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 promoter results inthe reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5\/POTH1 binding specificity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.ficity to themotifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggestthat, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.StBEL5 messenger RNA regulates other aspects of vegetative development.","palabras_clave":"Homeobox|mRNA transport|Gene expression|Plant development"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62775,"idioma_id":4,"titulo":"TCP transcription factors: architectures of plant form","resumen":"After its initial definition in 1999, the TCP family of transcription factors has become the focus of a multiplicity of studies related with plant development at the cellular, organ and tissue level. Evidence has accumulated indicating that TCP transcription factors are main regulators of plant form and architecture and constitute a tool through which evolution shapes plant diversity. TCP transcription factors act in a multiplicity of pathways related with cell proliferation and hormone responses. In recent years, molecular pathways of TCP protein action and biochemical studies on their mode of interaction with DNA have begun to shed light on their mechanism of action. However, the available information is fragmented and a unifying view of TCP protein action is lacking, as well as detailed structural studies of the TCP-DNA complex. Also important, the possible role of TCP proteins as integrators of plant developmental responses to the environment has deserved little attention. In this review, we summarize the current knowledge about the structure and functions of TCP transcription factors and analyze future perspectives for the study of the role of these proteins and their use to modify plant development.","palabras_clave":"Plant development|Transcription factor|TCP domain|Cell proliferation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62776,"idioma_id":4,"titulo":"Redox modulation of plant developmental regulators from the class I TCP transcription factor family","resumen":"TEOSINTE BRANCHED1-CYCLOIDEA-PROLIFERATING CELL FACTOR1 (TCP) transcription factors participate in plant developmental processes associated with cell proliferation and growth. Most members of class I, one of the two classes that compose the family, have a conserved cysteine at position 20 (Cys-20) of the TCP DNA-binding and dimerization domain. We show that Arabidopsis (Arabidopsis thaliana) class I proteins with Cys-20 are sensitive to redox conditions, since their DNAbinding activity is inhibited after incubation with the oxidants diamide, oxidized glutathione, or hydrogen peroxide or withnitric oxide-producing agents. Inhibition can be reversed by treatment with the reductants dithiothreitol or reduced glutathione or by incubation with the thioredoxin\/thioredoxin reductase system. Mutation of Cys-20 in the class I protein TCP15 abolished its redox sensitivity. Under oxidizing conditions, covalently linked dimers were formed, suggesting that inactivation is associated with the formation of intermolecular disulfide bonds. Inhibition of class I TCP protein activity was also observed in vivo, in yeast (Saccharomyces cerevisiae) cells expressing TCP proteins and in plants after treatment with redox agents. This inhibition was correlated with modifications in the expression of the downstream CUC1 gene in plants. Modeling studies indicated that Cys-20 is located at the dimer interface near the DNA-binding surface. This places this residue in the correctorientation for intermolecular disulfide bond formation and explains the sensitivity of DNA binding to the oxidation of Cys-20. The redox properties of Cys-20 and the observed effects of cellular redox agents both in vitro and in vivo suggest that class I TCPprotein action is under redox control in plants.","palabras_clave":"ARABIDOPSIS THALIANA|TCP TRANSCRIPTION FACTOR|DNA BINDINDG|REDOX REGULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62777,"idioma_id":4,"titulo":"Oxidation of carbohydrates of biological importance by the aquachromium(IV) ion","resumen":"The oxidation reactions kinetics of a series of related saccharides by aqua-oxo chromium(IV) ion, (H2-O)5CrIVO2+, were carried out in perchloric acid aqueous solutions. These reactions yield superoxochromium(III) ion, CrO22+, providing evidence that the two-electron reduction of CrO2+ to Cr2+ occurred in a single step. In all of these reactions, Cr2+ is the immediate product and could be trapped as CrO22+ when an excess of oxygen was present. The bimolecular rate constants for different aldoses and D-glucitol areindependent of [H+] in the range 0.1?1.0 M. Relative reactivities of these saccharides toward CrO2+ reduction are 1-methyl-a-D-glucopyranose << 1-methyl-a-D-alactopyranose _ 3-O-methyl-D-glucopyranose_ 6-desoxi-L-galactopyranose _ 2-desoxi-D-glucopyranose _ D-glucopyranose << D-galactopyranose<< D-glucitol. The oxidation of aldonic acid such as D-gluconic by CrO2+ showed the same mechanism but the redox process is strongly inhibitedwhen [H+] increases. Activation parameterswere also determined for selected reactions. On the basis of the kinetic result, activation parameters data and oxidized organic products, the mechanism of saccharides oxidation by CrO2+ is proposed to be a direct hydride-ion transfer.","palabras_clave":"CHROMIUM|SACCHARIDES|KINETICS|MECHANISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62778,"idioma_id":4,"titulo":"Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine","resumen":"In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-&#947; and IFN-&#945; from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.","palabras_clave":"Colostrum cells|qPCR relative method| VP6 subunit vaccine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62779,"idioma_id":4,"titulo":"?Metabolic interconnectivity among alternative respiration, residual respiration, carbohydrates and phenolics in leaves of Salvinia minima exposed to Cr(VI)","resumen":"Floating and submerged leaves of the aquatic fern Salvinia minima were used to analyze a metabolic interconnectivity among mitochondrial alternative respiration, residual respiration (Rresp), carbohydrate metabolism and soluble phenolics (SP) accumulation occurring under Cr(VI) stress. Treatment with Cr enhanced alternative pathway capacity (APcap) and (Rresp) in both leaf types. APcap\/Tresp ratio revealed an increasing relative contribution of the alternative respiration to total respiration rate under Cr(VI) treatment. Sucrose content increased in Cr-treated leaves, but glucose and starch decreased. Enzyme profile showed that sucrose synthase (SS) rather than soluble acid invertase (AI) seems to be involved in sucrose metabolism of Cr-treated plants. Accumulation of SP showed a positive correlation with both APcap and Rresp in floating leaves. Decreases of SP in submerged leaves can be explained by an increased synthesis of polymerized phenolics. Results provide important new insights about influence of alternative and residual respirations on the synthesis of phenylpropanoid-derivative compounds. This work could also represent the first communication about involvement of the Rresp in defence mechanism of S. minima against Cr(VI) toxicity. Salvinia minima were used to analyze a metabolic interconnectivity among mitochondrial alternative respiration, residual respiration (Rresp), carbohydrate metabolism and soluble phenolics (SP) accumulation occurring under Cr(VI) stress. Treatment with Cr enhanced alternative pathway capacity (APcap) and (Rresp) in both leaf types. APcap\/Tresp ratio revealed an increasing relative contribution of the alternative respiration to total respiration rate under Cr(VI) treatment. Sucrose content increased in Cr-treated leaves, but glucose and starch decreased. Enzyme profile showed that sucrose synthase (SS) rather than soluble acid invertase (AI) seems to be involved in sucrose metabolism of Cr-treated plants. Accumulation of SP showed a positive correlation with both APcap and Rresp in floating leaves. Decreases of SP in submerged leaves can be explained by an increased synthesis of polymerized phenolics. Results provide important new insights about influence of alternative and residual respirations on the synthesis of phenylpropanoid-derivative compounds. This work could also represent the first communication about involvement of the Rresp in defence mechanism of S. minima against Cr(VI) toxicity. ","palabras_clave":"Cr(VI)|Alternative pathway capacity|Residual respiration|Carbohydrates"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62780,"idioma_id":4,"titulo":"Formation of Au\/Pd-Sn trimetallic particles onto vitreous carbon","resumen":"The sequential deposition process of Pd and Sn on Au microcrystals, previously electrodeposited onto vitreous carbon (VC) was investigated in sulphuric acid solution by cyclic voltammetry and Auger electron spectroscopy (AES). Scanning electron microscopy was used to characterize Au deposits. The results reveal that the deposition of Pd and Sn is selective on the Au microcrystals, uniformly distributed on the VC surface, due to the strong interaction energy between the three metals. From the cyclic voltammetric and AES measurements in the VC\/Au\/Pd\/Sn system, the existence of a Sn core-shell structure with Pd can be inferred. Similar voltammetric results were obtained for the VC\/Au\/Sn\/Pd system, where in this case, the Pd adsorbed layer screened the Sn deposits on the VC\/Au interface. However, the AES spectrum shows that Sn is still present on the surface, indicating that probably, the Sn deposits have diffused to the surface, or they are not completely covered by Pd. The VC\/Au\/Pd\/Sn modified substrate appears as an interesting electrocatalyst material for the reduction of nitrate ions.","palabras_clave":"VITREOUS CARBON|TIN|PALLADIUM|GOLD|SPONTANEOUS DEPOSITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62781,"idioma_id":4,"titulo":"Holocene coastal environments and processes in subantarctic\/temperate cold Tierra del Fuego, Argentina-Chile","resumen":"Abstract: Holocene coastal environments in the Tierra del Fuego archipelago, southernmostSouth America, evolved in a temperate cold climate characterized by strong and persistent midlatitudewesterly winds, and under stable to falling relative sea-level. Southern and westerncoasts are rocky, microtidal and generally devoid of Holocene accretionary coastal landforms.They are young, having originated from flooding by the early Holocene. Northern and easterncoasts are lined by receding cliffs that supply abundant sediment to macro\/mesotidal embaymentslodging extensive accretionary coastal landforms. Spit growth on the Atlantic shore played a majorpart in modifying the development of other landforms by barring the shoreline from wave attack.Sediment was mainly supplied from Pleistocene glacial deposits exposed at eroding headlandsand, more locally, obtained by cannibalization and from elevated Pleistocene beaches. In places,westerly winds combined with Atlantic swell to foster spit growth under high-energy wavepower on both bay and ocean flanks ? a unique occurrence. In addition, the westerlies causedwidespread deflation on supratidal marshes and delivered fine-grained sediment inland and tothe sea. Recent acceleration of glacio-eustatic rise is gradually changing the coastal zone to a conditionof relative sea-level rise. The generally high and steep coastlines will not be greatly affected.A few gently-dipping lowlands might be flooded and biodiversity will be negatively affected.","palabras_clave":"BEACHES|GRAVEL|QUATERNARY|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62782,"idioma_id":4,"titulo":"Living with Kin in Lowland Horticultural Societies","resumen":"Classical social organization studies examine postmarital residence decisions by asking whether or not couples generally reside with or near particular relatives after marriage. Historically, the standard method was to note the ideal arrangement or the most common type of residence pattern and to ignore variation. However, it can be difficult clearly to distinguish actual decisions made by individuals and the on-the-ground availability of kin of different categories from preferences for particular residence situations that may not be realized due to demographic constraints. Our method using actual coresidence information may offer some advantages over standard anthropological typologies by reporting and comparing counts of coresident kin. Stated cultural ?rules? may still be informative, however, since our method can only clearly distinguish moves between segments within villages from coresidence in the natal house when censuses are specific to multiple scales of analysis. Comparatively, postmarital residence patterns in traditional human societies figure prominently in models of social evolution with arguments for patrilocal human bands similar in structure to female-dispersal systems in other hominid societies. However, considerable flexibility in hunter-gatherer cultures has led to their characterization as primarily multilocal. Horticulturalists are associated with larger, more sedentary social groups with more political inequality and intergroup conflict and may therefore provide additional insights into evolved human social structures. We analyze coresidence patterns of primary kin for 34 New World horticultural societies (6,833 adults living in 243 residential groupings) to show more uxorilocality (women live with more kin) than found for hunter-gatherers. Our findings further point to the uniqueness of human social structures and to considerable variation that is not fully described by traditional postmarital residence typologies. Sex biases in coresident kin can vary according to the scale of analysis (household vs. house cluster vs. village) and change across the life span, with women often living with more kin later in life. Headmen in large villages live with more close kin, primarily siblings, than do nonheadmen. Importantly, human marriage exchange and residence patterns create meta-group social structures, with alliances extending across multiple villages often united in competition against other large alliances at scales unparalleled by other species.","palabras_clave":"KINSHIP|LOWLAND SOUTH AMERICA|POSTMATRIMONIAL RESIDENCE|COMPARATIVE METHOD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62783,"idioma_id":4,"titulo":"Characterization of LIC11207, a novel leptospiral protein that is recognized by human convalescent sera and prevents apoptosis of polymorphonuclear leukocytes","resumen":"We report the study of a predicted outer-membrane leptospiral protein encoded by the gene lic11207.This protein is conserved in several pathogenic leptospiral strains but is absent in the saprophyte Leptospirabiflexa. This putative outer-membrane protein has a domain of unknown function (DUF) 1565found in several phylogenetically diverse bacteria and in the archaeon Methanosarcina acetivorans. Thegene was cloned and expressed in Escherichia coli BL21 (SI) strain using the expression vector pDEST17.The 34 kDa recombinant protein was tagged with N-terminal hexahistidine and purified by metalchargedchromatography. The purified protein was used to assess: reactivity with human convalescentsera; in vivo expression; ability to activate endothelial cells (EC); and ability to modulate the apoptosis ofpolymorphonuclear cells (PMNs). The LIC11207 coding sequence was identified in vivo in the hamsterrenal tubules during experimental infection with Leptospira interrogans. The rLIC11207 showed significantantigenicity against human convalescent sera when compared with sera from healthy donors. Therecombinant protein did not alter the surface expression of E-selectin or intercellular adhesion molecule1 (ICAM-1) in EC and failed to induce the release of von Willebrand factor (vWF). Interestingly, rLIC11207delayed apoptosis of PMNs suggesting a possible role of this protein during the infection","palabras_clave":"LIC11207|RECOMBINANT PROTEIN|LEPTOSPIROSIS|APOPTOSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62784,"idioma_id":5,"titulo":"Control predictivo para seguimiento de sistemas no lineales. Aplicación a una planta piloto.","resumen":"Este artículo trata el problema del diseño de un controlador predictivo para seguimiento de referencias cambiantes, en el caso de sistemas no lineales. Los controladores predictivos proveen leyes de control adecuadas para regular sistemas lineales o no lineales a un punto de equilibrio dado garantizando la satisfacción de restricciones y la estabilidad asintótica. Pero si este puntode equilibrio cambia, el controlador podría perder la estabilidad o incluso la factibilidad y por lo tanto sería incapaz de seguir la referencia deseada. En (Ferramosca et al., 2009a) se ha propuesto un controlador predictivo para seguimiento de referencias capaz de garantizar factibilidad y convergencia al punto de equilibrio a pesar de los cambios que este pueda sufrir. En este artículo, este controlador se utiliza para controlar en tiempo real una planta piloto de procesos. Los resultados obtenidos demuestran que el controlador predictivo para seguimiento es capaz de controlar plantas con dinámicas no lineales y restricciones. El experimento demuestra cómo el controlador garantiza estabilidad, factibilidad y convergencia también en caso de referencias no alcanzables.","palabras_clave":"CONTROL PREDICTIVO|SEGUIMIENTO DE REFERENCIAS|ESTABILIDAD|SISTEMAS NO LINEALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62785,"idioma_id":4,"titulo":"Cooperative distributed MPC for tracking","resumen":"This paper proposes a cooperative distributed linear model predictive control strategy for tracking changing setpoints, applicable to any finite number of subsystems. The proposed controller is able to drive the whole system to any admissible setpoint in an admissible way, ensuring feasibility under any change of setpoint. It also provides a larger domain of attraction than standard distributed MPC for regulation, due to the particular terminal constraint. Moreover, the controller ensures convergence to the centralized optimum, even in case of coupled constraints. This is possible thanks to the warm start used to initialize the optimization Algorithm, and to the design of the cost function, which integrates a Steady State Target Optimizer (SSTO). The controller is applied to a real 4 tanks plant.","palabras_clave":"MODEL PREDICTIVE CONTROL|DISTRIBUTED CONTROL|COOPERATIVE GAMES|SETPOINT TRACKING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62786,"idioma_id":4,"titulo":"Middle Cenozoic penguin remains from the Patagonian Cordillera","resumen":"Middle Cenozoic marine fossil-bearing beds outcrop in the Cerro Plataforma (western Chubut Province, Argentina) at about 1400 m a.s.l. They are located 500 km far from the modern Atlantic coast and only 50 km from the Pacific Ocean. Well preserved penguin bones and a shark tooth were found therein. Invertebrates include corals, bryozoans, abundant mollusks, echinoids, and crabs. Morphogeometric analysis and comparative description confirmed that the penguin humerus is referable to Palaeospheniscus bergi Moreno and Mercerat, 1891. Most of the fossils indicate a Miocene age. However, there is a debate about if the bearing beds are of Pacific or Atlantic origin. Fossil invertebrates identifiable at species level reveal Pacific affinity, the shark species is cosmopolitan, and Palaeospheniscus bergi is known from early Miocene Atlantic units of southern South America. Yet, Palaeospheniscus Moreno and Mercerat, 1891 is also known from Miocene Pacific beds but it has not been identified at specific level yet. This is the highest site in topographic terms in which penguin fossils occur. This indicates a remarkable uplift for the area, probably from the middle Miocene on.","palabras_clave":"Spheniscidae|Miocene|Patagonia|Fossil penguins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62787,"idioma_id":4,"titulo":"Transcript expression of vesicular glutamate transporters in lumbar dorsal root ganglia and the spinal cord of mice - Effects of peripheral axotomy or hindpaw inflammation","resumen":"Using specific riboprobes, we characterized the expression of VGLUT1-VGLUT3 transcripts in lumbar 4-5 (L4-5) DRGs and the thoracolumbar to lumbosacral spinal cord in male BALB\/C mice after a 1 or 3-day hindpaw inflammation, or a 7-day sciatic nerve axotomy. Sham animals were also included. In sham and contralateral L4-5 DRGs of injured mice, VGLUT1-, VGLUT2- and VGLUT3 mRNAs were expressed in ~45%,  ~69% or ~17% of all neuron profiles (NPs), respectively. VGLUT1 was expressed in large and some medium-sized NPs; VGLUT2 in neurons of all sizes, and VGLUT3 in small and medium-sized NPs. In the spinal cord, VGLUT1 was restricted to a number of neurons at thoracolumbar and lumbar levels, in what appears to be the dorsal nucleus of Clarke, and in mid laminae III-IV. In contrast, VGLUT2 was present in numerous NPs at all analyzed spinal levels, except the lateral aspects of the ventral horns, especially at the lumbar enlargement, were it was virtually absent. VGLUT3 was detected in a discrete number of neurons in laminae III-IV of the dorsal horn. Axotomy resulted in a moderate decrease in the number of DRG NPs expressing VGLUT3; VGLUT1 and VGLUT2 were unaffected. Likewise, VGLUTs transcripts remained unaltered after hindpaw inflammation, both in DRGs and the spinal cord. Altogether, these results confirm previous descriptions on VGLUTs, with the exception of VGLUT1, whose protein expression was detected in a lesser percentage of mouse DRG NPs. We also show a modest decrease in VGLUT3 transcript in DRGs. Lack of changes after injury for VGLUT1 and VGLUT2 transcripts (and VGLUT3 after hindpaw inflammation), as compared to data on protein expression, suggests translational\/trafficking over transcriptional regulations of VGLUTs after injury.","palabras_clave":"VGLUTs|DRGs|SPINAL CORD|AXOTOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62788,"idioma_id":4,"titulo":"A comprehensive approach to determine the probiotic potential of human-derived Lactobacillus for industrial use.","resumen":".","palabras_clave":"."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62789,"idioma_id":4,"titulo":"Multi-proxy evidence of Late Quaternary environmental changes in the coastal area of Puerto Lobos (northern Patagonia, Argentina)","resumen":"A multidisciplinary approach involving geomorphology, taxa composition of mollusks and taphonomy  performed on bivalve shells, linked to mineralogical and microstructural analyses, provide evidence of  Late Quaternary environmental changes in the coastal area of Puerto Lobos, in northern Patagonia. Digital  elevation models (DEMs) showed a beach ridge system developed parallel to the coast, indicating sea  level variations during MIS1, MIS5e, and earlier in the Pleistocene. These ridges can be correlated with  other marine ridges along the Patagonian coast, showing a similar geomorphological pattern and similar  temporal evolution. In relation to faunal composition, when comparing the Pleistocene with the Holocene,  the most relevant differences are the presence of Tegula atra and Mactra patagonica in the Pleistocene  sediments and a diversification of taxa in the Holocene. During the late Holocene, the coastal area  of Puerto Lobos also recorded a faunal shift in which species belonging to the Magellan Province displaced  the fauna of the Argentinean Province to the north, probably in coincidence with the Little Ice Age  (LIA). Taphonomic analysis on Glycymeris longior and Venus antiqua shells, two common taxa in these  beach ridges, indicate greater energy in the depositional environment correlated with the youngest  Holocene beach ridge. These shells exhibited taphonomic differences between them in relation to  fracturing, explained on the basis of higher elasticity in the G. longior shell than in V. antiqua shell,  because of its crossed lamellar microstructure.","palabras_clave":"geomorphology|paleontology|sea level"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62790,"idioma_id":4,"titulo":"Pharmacodynamic study of the 7,8-dihydroxy-4-methylcoumarin-induced selective cytotoxicity towards U-937 leukemic cells versus mature monocytes: cytoplasmic p21Cip1\/WAF1 as resistance factor","resumen":"Abstract  The development of tumor-selective drugs with low systemic toxicity has always been a major challenge in cancer treatment. Our group previously identified the 7,8-dihydroxy-4-methylcoumarin (DHMC) as a potential chemotherapeutic agent due to its potent, selective anti-proliferative and apoptosis-inducing effects on several cancer cell lines over peripheral blood mononuclear cells. However, there are still no published reports that can explain such selectivity of action. Herein, we addressed this question by using the U-937 promonocytic leukemia cell line, which can be forced to differentiate into a monocyte-like phenotype in vitro. U-937 cells differentiation is dependent on the nuclear expression of p21(Cip1\/WAF1), a protein that is absent in immature U-937 cells but present in both the nucleus and the cytoplasm of normal DHMC-resistant monocytes. Considering that induction of differentiation rendered U-937 cells resistant to DHMC, we evaluated the possible causal role of cytoplasmic p21(Cip1\/WAF1) in the onset of such resistance by employing U-937 cells stably transfected with a ZnCl2-inducible p21(Cip1\/WAF1) variant lacking the nuclear localization signal (U-937\/CB6-&#916;NLS-p21 cells). Expression of cytoplasmic p21(Cip1\/WAF1) did not induce differentiation of the cells but turned them resistant to DHMC through inhibition of JNK, a crucial mediator of DHMC-induced apoptosis in U-937 cells. Sub-acute toxicity evaluation of DHMC in Balb\/c mice indicated that DHMC administered intraperitoneally at doses up to 100mg\/kg induced no systemic damage. Collectively, our results explain for the first time the selective cytotoxicity of DHMC for tumor cells over normal monocytes, and encourage further in vivo studies on this compound as potential anti-leukemic agent.","palabras_clave":"Pharmacodynamics|Coumarin|Anti-Leukemic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62791,"idioma_id":5,"titulo":"El sistema vomeronasal y su posible funcionalidad en larvas de anuros","resumen":"El sistema vomeronasal (SVN) es un sistema olfatorio accesorio presente en la mayoría de los tetrápodos. Clásicamente se lo ha asociado con el paso de los vertebrados al ambiente terrestre, sin embargo las evidencias surgidas en los últimos años indican que el SVN apareció tempranamente en la evolución de los tetrápodos y sería funcional en ambientes acuáticos. Este sistema sensorial ha sido descripto en etapas larvales de anuros. Pero ¿es funcional el SVN en renacuajos? No existen experimentos en donde se evalúe la participación del SVN en la quimiodetección en renacuajos. Sin embargo, un número considerable de evidencias indican que este sistema sensorial podría ser funcional en larvas de anuros: 1) El órgano vomeronasal (OVN) aparece durante el desarrollo embrionario y esta presente durante toda la etapa larval. 2) El OVN contiene neuronas bipolares cuyos axones proyectan al bulbo olfatorio accesorio (BOA) donde establecen conexiones sinápticas con neuronas telencefálicas. 3) Las neuronas del OVN expresan los receptores de membrana descriptos en tetrápodos, así como la proteína G involucrada en la señalización intracelular. 4) Los análisis de microscopía electrónica demuestran que el OVN posee neuronas con microvellosidades apicales como se describe para otros grupos y sus características ultraestructurales no se modifican durante la metamorfosis. Más aún, no hay diferencias en la ultraestructura de las conexiones sinápticas entre larvas y adultos a nivel del BOA. Los renacuajos presentan una gran cantidad de comportamientos mediados por quimiodetección. Conocer si el SVN participa en la detección de alguno\/s de estos estímulos ayudaría a dilucidar no solo aspectos relacionados con la comunicación química y el comportamiento en renacuajos sino también comprender aspectos evolutivos de los sistemas quimiosensoriales en vertebrados. ","palabras_clave":"QUIMIODETECCIÓN|ÓRGANO VOMERONASAL|RENACUAJO|FEROMONA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62792,"idioma_id":4,"titulo":"Characterization, dissolution and in vivo evaluation of solid acetazolamide complexes","resumen":"The effects of binary and ternary systems of acetazolamide (ACZ) with hydroxypropyl-b-cyclodextrin (HP-b-CD) alone or with triethanolamine (TEA) on the crystalline properties, dissolution and intraocular pressure (IOP)-lowering effect were investigated.    It was found that the crystal structure of ACZ powder could be modified by the processing conditions. Freeze-drying ACZ powder affected not only the particle morphology but also its polymorphic form and the starting ACZ was converted to pure form A upon freeze-drying treatment. Results provided by DSC\/TGA, XRPD, SEM and FT-IR suggested the formation of inclusion complexes between ACZ with HP-b-CD alone or with TEA, obtained by the freeze-drying method and the conversion of the drug into the amorphous state. Binary and ternary systems of ACZ obtained by freeze-drying exhibited significantly enhanced ACZ dissolution rates. The IOP-lowering effects of ACZ and its complexes with HP-b-CD alone or with TEA were studied in normotensive rabbits. Whereas the maximum IOP-lowering effect (4 mmHg, 33%), obtained with these binary and ternary lyophilized ACZ systems occurred at around 90 min, the ternary system exhibited a longer maximum IOP-lowering effect peak compared with that of the binary system. These results are in line with those obtained from the dissolution studies, where the ternary system exhibited longer dissolution times compared to the lyophilized binary one.    Results obtained from the dissolution studies, also showed that freeze-drying the native crystalline form of ACZ significantly increased the dissolution rate of ACZ, thus improving the IOP-lowering effect of this drug.","palabras_clave":"Acetazolamide|Ciclodextrin|Intraocular Pressure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62793,"idioma_id":4,"titulo":"Mathematical Modeling of Bivariate Distributions of Polymer Properties Using 2-D Probability Generating Functions. Part II: Transformation of Population Mass Balances of Polymer Processes","resumen":"This is the second of two works presenting a newmathematical method for modeling bivariate distributions of polymer properties. It is based on the transformation of population balances using 2D probability generating functions (pgf) and a posteriori recovery of the distribution from the transform domain by numerical inversion. Part I of this work was devoted to the numerical inversion step. Here the transformation of the population balances to the pgf domain is analyzed. A 2D pgf transform table is developed, which allows a simple transformation of any typicalpolymer balance equation. Three copolymerization examples are used to show the application of the complete procedure of this modeling technique.","palabras_clave":"BIVARIATE DISTRIBUTION|MODELING MOLECULAR WEIGHT|DISTRIBUTION\/MOLAR MASS DISTRIBUTION|PROBABILITY GENERATING FUNCTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62794,"idioma_id":4,"titulo":"Effect of cholesterol-poly(N,N-dimethylaminoethyl methacrylate) on the properties of stimuli-responsive polymer liposome complexes","resumen":"The development of new polymer\u2013liposome complexes (PLCs) as delivery systems is the key issue  of this work. Three main areas are dealt with: polymer synthesis\/characterization, liposome formulation\/  characterization and evaluation of the PLCs uptake by eukaryotic cells.  Poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) with low molecular weight and narrow  polydispersity was synthesized by Atom Transfer Radical Polymerization (ATRP). The polymers were  synthesized using two different bromide initiators (cholesteryl-2-bromoisobutyrate and ethyl 2-  bromoisobutyrate) as a route to afford PDMAEMA and CHO-PDMAEMA.  Both synthesized polymers (PDMAEMA and CHO-PDMAEMA) were incorporated in the preparation  of lecithin liposomes (LEC) to obtain PLCs. Three polymer\/lipid ratios were investigated: 5, 10 and 20%. Physicochemical characterization of PLCs was carried out by determining the zeta potential, particle  size distribution, and the release of fluorescent dyes (carboxyfluorescein CF and calcein) at different  temperatures and pHs.  The leakage experiments showed that CHO covalently bound to PDMAEMA strongly stabilizes PLCs. The  incorporation of 5% CHO-PDMAEMA to LEC (LEC CHO-PD5) appeared to be the stablest preparation at pH  7.0 and at 37 &#9702;C. LEC CHO-PD5 destabilized upon slight changes in pH and temperature, supporting the  potential use of CHO-PDMAEMA incorporated to lecithin liposomes (LEC CHO-PDs) as stimuli-responsive  systems.  In vitro studies on Raw 264.7 and Caco-2\/TC7 cells demonstrated an efficient incorporation of PLCs into  the cells. No toxicity of the prepared PLCs was observed according to 3-(4,5-dimethylthiazol-2-yl)-2,5-  diphenyltetrazolium bromide (MTT) assays. These results substantiate the efficiency of CHO-PDMAEMA  incorporated onto LEC to assist for the release of the liposome content in mildly acidic environments, like  those found in early endosomes where pH is slightly lower than the physiologic.  In summary, the main achievements of this work are: (a) novel synthesis of CHO-PDMAEMA by ATRP,  (b) stabilization of LEC by incorporation of CHO-PDMAEMA at neutral pH and destabilization upon slight  changes of pH, (c) efficient uptake of LEC CHO-PDs by phagocytic and non-phagocytic eukaryotic cells.","palabras_clave":"CHO-PDMAEMA|Polymer\u2013lipid complexes|Stimuli responsive-liposome|Liposome internalization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62795,"idioma_id":4,"titulo":"Thermospermine catabolism increases Arabidopsis thaliana resistance to Pseudomonas viridiflava","resumen":"This work investigated the roles of the tetraamine thermospermine (TSpm) by analysing its contribution to Arabidopsis basal defence against the biotrophic bacterium Pseudomonas viridiflava. The participation of polyamine oxidases (PAOs) in TSpm homeostasis and TSpm-mediated defence was also investigated. Exogenous supply of TSpm, as well as ectopic expression of the TSpm biosynthetic gene ACL5, increased Arabidopsis Col-0 resistance to P. viridiflava, while null acl5 mutants were less resistant than Col-0 plants. The above-mentioned increase in resistance was blocked by the PAO inhibitor SL-11061, thus demonstrating the participation of TSpm oxidation. Analysis of PAO genes expression in transgenic 35S::ACL5 and Col-0 plants supplied with TSpm suggests that PAO 1, 3, and 5 are the main PAOs involved in TSpm catabolism. In summary, TSpm exhibited the potential to perform defensive functions previously reported for its structural isomer Spm, and the relevance of these findings is discussed in the context of ACL5 expression and TSpm concentration in planta. Moreover, this work demonstrates that manipulation of TSpm metabolism modifies plant resistance to pathogens.","palabras_clave":"Arabidopsis thaliana|Pseudomonas viridiflava|Polyamines|Thermospermine|ACAULIS5|Polyamine oxidase|Defense"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62796,"idioma_id":4,"titulo":"Antiferromagnetism and ferromagnetism in layered 1T-CrSe2 with V and Ti replacements","resumen":"We have studied the effect of substitution of Cr in metastable 1T-CrSe2 by Ti and V on its structural and magnetic properties. The structural   transitions observed between 165-180 K in the pure material are stomped   by the doping. The pure compound has a magnetization corresponding to an   antiferromagnetic (AF) ground state. On Ti substitution, we observe an   increase of the lattice constants and a gradual passage towards a   ferromagnetic state, while V replacement maintains AF order up to our   highest doping, x_V=0.5. With our experimental results and the help of first-principles calculations, we construct the phase diagram of the system.  ","palabras_clave":"Magnetismo|Propiedades electrónicas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62797,"idioma_id":4,"titulo":"Adherence of model molecules to silica surfaces: first principle calculations.","resumen":"The adherence of model molecules to&nbsp; crystal SiO2 surfaces is studied from first principle calculations at the DFT level. Adsorption energies are calculated which follow the experimental threads obtained elsewhere (Rivera et al.,2013). We study the quantum nature of the electronic charge transfer between the surface and the molecules, showing the localized and delocalized patterns associated to the repulsive and attractive case respectively.","palabras_clave":"Glass surface|Adherence|Methylene blue"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62798,"idioma_id":4,"titulo":"Adherence of molecules to silica glass surface: Experimental results and theoretical calculations","resumen":"The adherence of molecules to surfaces is used in a wide scope of technological applications. In this work we study the attachment of molecules onto porous silica glass surface obtained from a Vycor glass, after glassglass phase separation and leaching of the soluble phase with water. Nitrogen adsorption at 77 K was used for the determination of the specific surface area (BET area) and pore size distribution on the leached glass. The adherence onto the glass surface, of model molecules methylene blue (MB) and eosine yellow (EY), with positive and negative electrical charges respectively, was studied. The adsorption kinetics was determined from aqueous solutions using UV-VIS spectroscopy. It was found that the silica glass surface as prepared in of glass was found for methylene blue, and almost null adsorption for eosine yellow. First principles calculations were performed using the Density Functional Theory in order to model the interaction between both molecules and the glass surface.","palabras_clave":"Glass Surface|Adherence|Doxorubicina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62799,"idioma_id":5,"titulo":"Estado de avance de las investigaciones arqueológicas en el sitio Empalme Querandíes 1 (centro de la subregión Pampa Húmeda, Provincia de Buenos Aires)","resumen":"El objetivo de este trabajo es presentar el estado de avance de las distintas líneas de evidencia (geoambiental, cronología, tafonomía, subsistencia y tendencias tecnológicas) obtenidas a partir de las investigaciones arqueológicas llevadas a cabo en el sitio Empalme Querandíes 1 (centro de la subregión Pampa Húmeda, provincia de Buenos Aires). Los estudios estratigráficos y tafonómicos sugieren que el contexto presenta una baja integridad y resolución, abarcando diferentes eventos de ocupación del Holoceno tardío inicial (entre ca. 3100-2000 años AP). El análisis faunístico evidencia que el guanaco, el venado de las pampas, el ñandú y cuatro especies de armadillos formaron parte de la dieta. Asimismo, los restos óseos de por lo menos las dos primeras especies fueron utilizados como materia prima para confeccionar instrumentos. Respecto del material lítico, las rocas mejor representadas son la ortocuarcita y la ftanita, observándose diferencias en los objetivos de producción y en las estrategias vinculadas con su utilización. En el caso de la cerámica, se registró la presencia de tiestos no decorados y decorados con motivos geométricos. Las distintas líneas de evidencia sugieren que parte de las ocupaciones de este sitio multicomponente estarían vinculadas con loci de actividades múltiples de campamentos base.","palabras_clave":"HOLOCENO TARDÍO INICIAL|CAZADORES-RECOLECTORES|GEOAMBIENTE|ZOOARQUEOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62800,"idioma_id":4,"titulo":"Genetic and Antigenic Evolution Profiles of G1 Rotaviruses in Co´rdoba, Argentina, During a 27-Year Period (1980?2006)","resumen":"Rotavirus G1 strains represent the most common genotype that causes diarrhea in humans and has been incorporated into both, monovalent and multivalent, rotavirus licensed vaccines. The aim of this study was to determine the evolution profile of G1 rotaviruses in Córdoba, Argentina, over a 27-year period (1980-2006). Intragenotype diversity, represented by lineages within rotavirus circulating strains, was observed. Phylogenetic analysis of the VP7-gene of G1 rotavirus clinical strains showed the circulation of G1 lineage IV and V strains in the 1980s, and co-circulation of lineage I and II strains in the 1990s and 2000-2006. The distribution of G1 in lineages could be linked to multiple nucleotide substitutions distributed across lineages that did not correlate with the emergence of G1 antigenic variants. Moreover, temporal lineage distribution was not linked to significant changes in G1 prevalence. Therefore, the continuous and dominant circulation of G1 over time could not be related to the emergence of antigenic variants in the community. Continuous rotavirus surveillance is necessary to understand rotavirus evolution and to measure how genetic and antigenic changes might affect the effectiveness of vaccines in the future.","palabras_clave":"Rotavirus|G1 genotype|Phylogeny"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62801,"idioma_id":4,"titulo":"Osteosarcoma of the jaw: An analysis of a series of 74 cases","resumen":"AIMS:       To analyse a  series of cases of osteosarcoma of the jaw.      METHODS AND RESULTS:       The study  included 74 cases of osteosarcoma of the jaw.  Their clinical, radiographic and histopathological features were analysed, and  their frequency with respect to aggressive and malignant pathologies of the jaw was determined. Survival was assessed in 17 cases with available follow-up. Osteosarcoma  of the jaw accounted for 10% of primary malignant  and aggressive tumours of the jaw, and for 8% of  all malignant lesions of the jaw, including  metastatic and lymphoproliferative tumours. The mean age was 43 ± 18 years.  Radiographic features varied greatly and were non-specific, with a predominance  of mixed images. The dominant histological pattern was osteoblastic (48.4%),  followed by chondroblastic (37.1%). The survival rate at 5 years was 68%.  Females and patients with a predominantly chondroblastic pattern had lower  survival rates.","palabras_clave":"jaw|osteosarcoma|sarcoma"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62802,"idioma_id":4,"titulo":"Altered microRNA expression levels in mononuclear cells of patients with pulmonary and pleural tuberculosis and their relation with components of the immune response","resumen":"Different lines of evidence demonstrate that microRNAs (miRNAs) play an important role in host?pathogen interactions. In this study we investigated the expression patterns of several miRNAs, most of them involved in regulating inflammatory responses, in patients with tuberculosis (TB). In order to understand the events occurring at the site of infection, we employed mononuclear cells obtained from both peripheral blood (PBMC) and pleural fluids (PFMC) of patients. Interestingly, we found that the miRNA signature of each compartment is different, with a strong down-regulation in PFMCs of miR-223, miR-144* and miR-421. In addition, we observed that miR-146a expression is also down-regulated in tuberculosis patients, both in PBMCs and PFMCs while miR-424 levels are elevated only in the peripheral compartments. We also showed that systemic expression of these miRNAs changes upon specific treatment and is associated with IL-6 levels, a cytokine playing a substantial role in TB immunopathology. Present results contribute to a better knowledge of the host responses in TB pathogenesis, pointing out the role of miRNAs in this disease.","palabras_clave":"Tuberculosis|microRNA expression|Immunopathology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62803,"idioma_id":4,"titulo":"Adverse neuro-immune-endocrine interactions in patients with active tuberculosis","resumen":"    The nervous, endocrine and immune  systems play a crucial role in maintaining homeostasis and interact with each  other for a successful defensive strategy against injurious agents. However,  the situation is different in long-term diseases with marked inflammation, in  which defensive mechanisms become altered. In the case of tuberculosis (TB), this  is highlighted by several facts: an imbalance of plasma immune and endocrine mediators,  that results in an adverse environment for mounting an adequate response  against mycobacteria and controlling inflammation; the demonstration that dehidroepiandrosterone  (DHEA) secretion by a human adrenal cell line can be inhibited by culture  supernatants from Mycobacterium  tuberculosis-stimulated peripheral blood mononuclear cells \u2013PBMC- of TB  patients, with this effect being partly reverted when neutralizing transforming  growth factor-b in such supernantants; the in vitro effects of adrenal steroids on  the specific immune response of PBMC from TB patients, that is a cortisol  inhibition of mycobacterial antigen-driven lymphoproliferation and interferon-g production as well as a suppression of TGF-b production in DHEA-treated PBMC; lastly the demonstration that  immune and endocrine compounds participating in the regulation of energy  sources and immune activity correlated with the consumption state of TB  patients. Collectively, immune-endocrine disturbances of TB patients are involved in  critical components of disease pathology with  implications in the impaired clinical status and unfavourable disease outcome.  ","palabras_clave":"TUBERCULOSIS|NEUROSCIENCE|IMMUNE-ENDOCRINE INTERACTIONS|REGULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62804,"idioma_id":4,"titulo":"Tumor Necrosis Factor-a regulates glucocorticoid synthesis in the adrenal glands of Trypanosoma cruzi acutely-infected mice. The role of TNF-R1","resumen":"Adrenal steroidogenesis is under a complex regulation involving extrinsic and intrinsic adrenal factors. TNF-a is an inflammatory cytokine produced in response to tissue injury and several other stimuli. We have previously demonstrated that TNF-R1 knockout (TNF-R12\/2) mice have a dysregulated synthesis of glucocorticoids (GCs) during Trypanosoma cruzi acute infection. Since TNF-a may influence GCs production, not only through the hypothalamus-pituitary axis, but also at the adrenal level, we now investigated the role of this cytokine on the adrenal GCs production. Wild type (WT) and TNF-R12\/2 mice undergoing acute infection (Tc-WT and Tc-TNF-R12\/2 groups), displayed adrenal hyperplasia together with increased GCs levels. Notably, systemic ACTH remained unchanged in Tc-WT and Tc-TNF-R12\/2 compared with uninfectedmice, suggesting some degree of ACTH-independence of GCs synthesis. TNF-a expression was increased within the adrenal gland from both infected mouse groups, with Tc-WT mice showing an augmented TNF-R1 expression. Tc-WT mice showed increased levels of P-p38 and P-ERK compared to uninfected WT animals, whereas Tc-TNF-R12\/2 mice had increased p38and JNK phosphorylation respect to Tc-WT mice. Strikingly, adrenal NF-kB and AP-1 activation during infection was blunted in Tc-TNF-R12\/2 mice. The accumulation of mRNAs for steroidogenic acute regulatory protein and cytochrome P450 were significantly increased in both Tc-WT and Tc-TNF-R12\/2 mice; being much more augmented in the latter group, which alsohad remarkably increased GCs levels. TNF-a emerges as a potent modulator of steroidogenesis in adrenocortical cells during T. cruzi infection in which MAPK pathways, NF-kB and AP-1 seem to play a role in the adrenal synthesis of pro-inflammatory cytokines and enzymes regulating GCs synthesis. These results suggest the existence of an intrinsic immune-adrenal interaction involved in the dysregulated synthesis of GCs during murine Chagas disease.","palabras_clave":"Glucocorticoides|Factor de Necrosis tumoral|Adrenales|Trypanosoma cruzi"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62805,"idioma_id":4,"titulo":"Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes","resumen":"In experimental Trypanosoma cruzi infections, severe thymic atrophy leads to release of activated CD4 CD8 double-positive (DP) T cellsto the periphery. In humans, activated DP T cells are found in the blood in association with severe cardiac forms of human chronic Chagasdisease. The mechanisms underlying the premature thymocyte release during the chagasic thymic atrophy remain elusive. We tested whether themigratory properties of intrathymic thymocytes are modulated by the parasite trans-sialidase (TS). We found that TS affected the dynamics ofthymocytes undergoing intrathymic maturation, and these changes were accompanied by an increase in the number of recent DP thymic emigrantsin the peripheral lymphoid organs. We demonstrated that increased percentages of blood DP T cell subsets were associated with augmented antibody titers against TS in chagasic patients with chronic cardiomyopathy. In vitro studies showed that TS was able to activate theMAPK pathway and actin filament mobilization in thymocytes. These effects were correlated with its ability to modulate the adhesion ofthymocytes to thymic epithelial cells and their migration toward extracellular matrix. These findings point to effects of TS that could influence the escape of immature thymocytes in Chagas disease.","palabras_clave":"Trans-sialidasa|Trypanosoma cruzi|migración|miocardiopatía"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62806,"idioma_id":4,"titulo":"Computing ideal classes representatives in quaternion algebras","resumen":"Let $K$ be a totally real number field and let $B$ be a totally definitequaternion algebra over $K$. In this article, given a set of representatives forideal classes for a maximal order in $B$, we show how to construct in anefficient way a set of representatives of ideal classes for any Bass order in$B$. The algorithm does not require any knowledge of class numbers, and improvesthe equivalence checking process by using a simple calculation with globalunits. As an application, we compute ideal classes representatives for an orderof level $30$ in an algebra over the real quadratic field $Q[sqrt{5}]$.","palabras_clave":"Quaternion algebras|ideal class representatives|Bass orders"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62807,"idioma_id":4,"titulo":"Measurement of hard double-parton interactions in W->lv+ 2 jet events at sqrt(s)=7 TeV with the ATLAS detector","resumen":"The production of W bosons in association with two jets in proton\u2013proton collisions at a centre-of-mass energy of  has been analysed for the presence of double-parton interactions using data corresponding to an integrated luminosity of 36 pb&#8722;1, collected with the ATLAS detector at the Large Hadron Collider. The fraction of events arising from double-parton interactions, f(D)DP, has been measured through the pT balance between the two jets and amounts to f(D)DP = 0.08 ± 0.01 (stat.) ± 0.02 (sys.) for jets with transverse momentum pT > 20 GeV and rapidity |y| < 2.8. This corresponds to a measurement of the effective area parameter for hard double-parton interactions of &#963;eff = 15 ± 3 (stat.) +5&#8722;3 (sys.) mb.","palabras_clave":"Double-parton interactions|ATLAS|W boson"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62808,"idioma_id":4,"titulo":"Measurements of Wgamma and Zgamma production in pp collisions at sqrt{s}= 7 TeV with the ATLAS detector at the LHC","resumen":"The integrated and differential fiducial cross sections for the production of a W or Z boson in association with a high-energy photon are measured using pp collisions at sqrt{s} = 7 TeV. The analyses use a data sample with an integrated luminosity of 4.6 fb^{-1} collected by the ATLAS detector during the 2011 LHC data-taking period. Events are selected using leptonic decays of the W and Z bosons (W(e nu,mu nu) and Z(e+ e-, mu+ mu-, nu nubar)) with the requirement of an associated isolated photon. The data are used to test the electroweak sector of the Standard Model and search for evidence for new phenomena. The measurements are used to probe the anomalous WWgamma, ZZgamma and Zgammagamma triple-gauge-boson couplings and to search for the production of vector resonances decaying to Zgamma and Wgamma. No deviations from Standard Model predictions are observed and limits are placed on anomalous triple-gauge-boson couplings and on the production of new vector meson resonances.","palabras_clave":"ATLAS|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62809,"idioma_id":4,"titulo":"Measurement of kT splitting scales in W->lv events at sqrt(s)=7 TeV with the ATLAS detector","resumen":"A measurement of splitting scales, as defined by the kT clustering algorithm, is presented for final states containing a W boson produced in proton--proton collisions at a centre-of-mass energy of 7 TeV. The measurement is based on the full 2010 data sample corresponding to an integrated luminosity of 36 pb-1 which was collected using the ATLAS detector at the CERN Large Hadron Collider. Cluster splitting scales are measured in events containing W bosons decaying to electrons or muons. The measurement comprises the four hardest splitting scales in a kT cluster sequence of the hadronic activity accompanying the W boson, and ratios of these splitting scales. Backgrounds such as multi-jet and top-quark-pair production are subtracted and the results are corrected for detector effects. Predictions from various Monte Carlo event generators at particle level are compared to the data. Overall, reasonable agreement is found with all generators, but larger deviations between the predictions and the data are evident in the soft regions of the splitting scales.","palabras_clave":"W boson|KT splitting|ATLAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62810,"idioma_id":4,"titulo":"Measurement of the cross-section for W boson production                         in association with b-jets in pp collisions at $\\sqrt{s}$   = 7 TeV with the ATLAS detector","resumen":"This paper reports a measurement of the W +b-jets production cross-section in proton?proton colli-sions at a centre-of-mass energy of 7 TeV at the LHC. These results are based on data correspondingto an integrated luminosity of 4.6 fb&#8722;1 , collected with the ATLAS detector. Cross-sections are pre-sented as a function of jet multiplicity and of the transverse momentum of the leading b-jet for boththe muon and electron decay modes of the W boson. The W +b-jets cross-section, corrected for allknown detector effects, is quoted in a limited kinematic range, using jets reconstructed with the anti-ktclustering algorithm with transverse momentum above 25 GeV and rapidity within ±2.1. Combining themuon and electron channels, the fiducial cross-section for W +b-jets is measured to be 7.1 ± 0.5 (stat)± 1.4 (syst) pb, consistent with next-to-leading order QCD calculations within 1.5 standard deviations.","palabras_clave":"ATLAS|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62811,"idioma_id":4,"titulo":"Search for a light charged Higgs boson in the decay channel H+->csbar in ttbar events using pp collisions at sqrt(s) = 7 TeV with the ATLAS detector","resumen":"A search for a charged Higgs boson (H+) in ttbar decays is presented, where one of the top quarks decays via t->H+b, followed by H+->two jets (csbar). The other top quark decays to Wb, where the W boson then decays into a lepton (e\/mu) and a neutrino. The data were recorded in pp collisions at sqrt(s) = 7TeV by the ATLAS detector at the LHC in 2011, and correspond to an integrated luminosity of 4.7 fb-1. With no observation of a signal, 95% confidence level (CL) upper limits are set on the decay branching ratio of top quarks to charged Higgs bosons varying between 5% and 1% for H+ masses between 90 GeV and 150 GeV, assuming Br(H+->csbar)=100%.","palabras_clave":"ATLAS|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62812,"idioma_id":4,"titulo":"Improved luminosity determination in pp collisions at sqrt(s) = 7 TeV using the ATLAS detector at the LHC","resumen":"The luminosity calibration for the ATLAS detector at the LHC during pp collisions at sqrt(s) = 7 TeV in 2010 and 2011 is presented. Evaluation of the luminosity scale is performed using several luminosity-sensitive detectors, and comparisons are made of the long-term stability and accuracy of this calibration applied to the pp collisions at sqrt(s) = 7 TeV. A luminosity uncertainty of Delta L\/L = +\/- 3.5% is obtained for the 47 pb-1 of data delivered to ATLAS in 2010, and an uncertainty of Delta L\/L = +\/- 1.8% is obtained for the 5.5 fb-1 delivered in 2011.","palabras_clave":"ATLAS|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62813,"idioma_id":4,"titulo":"Search for WH production with a light Higgs boson decaying to prompt electron-jets in proton-proton collisions at $\\sqrt{s}$=7 TeV with the ATLAS detector","resumen":"A search is performed for W H production with a light Higgs boson decaying to hidden-sectorparticles resulting in clusters of collimated electrons, known as electron-jets. The search is performedwith 2.04 fb&#8722;1 of data collected in 2011 with the ATLAS detector at the LHC in proton?proton collisions&#8730;at s = 7 TeV. One event satisfying the signal selection criteria is observed, which is consistent withthe expected background rate. Limits on the product of the W H production cross section and thebranching ratio of a Higgs boson decaying to prompt electron-jets are calculated as a function of aHiggs boson mass in the range from 100 GeV to 140 GeV.","palabras_clave":"ATLAS|PARTICLE|PHYSICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62814,"idioma_id":4,"titulo":"Search for third generation scalar leptoquarks in pp collisions at sqrt(s) = 7 TeV with the ATLAS detector","resumen":"A search for pair-produced third generation scalar leptoquarks is presented, using proton?proton&#8730;collisions at s = 7 TeV at the LHC. The data were recorded with the ATLAS detector and correspondto an integrated luminosity of 4.7 fb&#8722;1 . Each leptoquark is assumed to decay to a tau lepton and ab-quark with a branching fraction equal to 100%. No statistically significant excess above the Stan-dard Model expectation is observed. Third generation leptoquarks are therefore excluded at 95%confidence level for masses less than 534 GeV.","palabras_clave":"ATLAS|PARTICLE|PHYSICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62815,"idioma_id":4,"titulo":"Measurement of the inclusive jet cross section in pp collisions at &#8730;s = 2.76 TeV and comparison to the inclusive jet cross section at &#8730;s = 7 TeV using the ATLAS detector","resumen":"The inclusive jet cross-section has been measured in proton-proton collisions at sqrt(s)=2.76 TeV in a dataset corresponding to an integrated luminosity of 0.20pb-1 collected with the ATLAS detector at the Large Hadron Collider in 2011. Jets are identified using the anti-kt algorithm with two radius parameters of 0.4 and 0.6. The inclusive jet double-differential cross-section is presented as a function of the jet transverse momentum pT and jet rapidity y, covering a range of 20 <= pT < 430 GeV and |y| < 4.4. The ratio of the cross-section to the inclusive jet cross-section measurement at sqrt(s)=7 TeV, published by the ATLAS Collaboration, is calculated as a function of both transverse momentum and the dimensionless quantity xT = 2 pT \/ sqrt(s), in bins of jet rapidity. The systematic uncertainties on the ratios are significantly reduced due to the cancellation of correlated uncertainties in the two measurements. Results are compared to the prediction from next-to-leading order perturbative QCD calculations corrected for non-perturbative effects, and next-to-leading order Monte Carlo simulation. Furthermore, the ATLAS jet cross-section measurements at sqrt(s)=2.76 TeV and sqrt(s)=7 TeV are analysed within a framework of next-to-leading order perturbative QCD calculations to determine parton distribution functions of the proton, taking into account the correlations between the measurements.","palabras_clave":"Jets|Cross-section|ATLAS|Hadron collisions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62816,"idioma_id":4,"titulo":"Measurement of the production cross section of jets in association with a Z boson in pp collisions at &#8730;s = 7 TeV with the ATLAS detector","resumen":"Measurements of the production of jets of particles in association with a Z boson in pp collisions at sqrt(s) = 7 TeV are presented, using data corresponding to an integrated luminosity of 4.6\/fb collected by the ATLAS experiment at the Large Hadron Collider. Inclusive and differential jet cross sections in Z events, with Z decaying into electron or muon pairs, are measured for jets with transverse momentum pT > 30 GeV and rapidity |y| < 4.4. The results are compared to next-to-leading-order perturbative QCD calculations, and to predictions from different Monte Carlo generators based on leading-order and next-to-leading-order matrix elements supplemented by parton showers.","palabras_clave":"Z boson|Jets|ATLAS|pQCD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62817,"idioma_id":4,"titulo":"Triggers for displaced decays of long-lived neutral particles in the ATLAS detector","resumen":"A set of three dedicated triggers designed to detect long-lived neutral particles decaying throughout the ATLAS detector to a pair of hadronic jets is described. The efficiencies of the triggers for selecting displaced decays as a function of the decay position are presented for simulated events. The effect of pile-up interactions on the trigger efficiencies and the dependence of the trigger rate on instantaneous luminosity during the 2012 data taking period at the LHC are discussed.","palabras_clave":"ATLAS|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62818,"idioma_id":4,"titulo":"Measurement of the high-mass Drell--Yan differential cross-section in pp collisions at sqrt(s)=7 TeV with the ATLAS detector","resumen":"This Letter reports a measurement of the high-mass Drell-Yan differential cross-section in proton-proton collisions at a centre-of-mass energy of 7 TeV at the LHC. Based on an integrated luminosity of 4.9 \/fb, the differential cross-section in the Z\/gamma* to e+e- channel is measured with the ATLAS detector as a function of the invariant mass, Mee, in the range 116  25 GeV and pseudorapidity eta < 2.5. A comparison is made to various event generators and to the predictions of perturbative QCD calculations at next-to-next-to-leading order.","palabras_clave":"ATLAS|LHC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62819,"idioma_id":4,"titulo":"Production and characterization of 'Escherichia coli' glycerol dehydrogenase as a tool for glycerol recycling","resumen":"    NAD+-dependent  glycerol (Gro) dehydrogenase (GroDHase) catalyzes the conversion of Gro into  dihydroxyacetone (DHA), the first step for fermentative Gro metabolism in Escherichia  coli. In this work, we cloned the gldA gene that codes for the E. coli  GroDHase and homologously expressed, purified, and kinetically characterized  the recombinant protein. To achieve this, the enzyme was over-produced using  Gro supplemented growth medium and lactose as the inducer. The enzyme was  highly purified using either pseudo-affinity chromatography or a simple  heat-shock treatment, which is potentially valuable for industrial production  of GroDHase. We detected efficient oxidation of Gro derived from biodiesel  production to DHA by gas chromatography. The results presented in this work  support recombinant GroDHase production in a biorefinery setting as a relevant  tool for converting Gro into DHA for future biotechnological applications.  ","palabras_clave":"BIODIESEL|BIOREFINERY|BIOTECHNOLOGY|DIHYDROXYACETONE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62820,"idioma_id":4,"titulo":"Remating behavior in Anastrepha fraterculus (Diptera: Tephritidae) females is affected by male juvenile hormone analog treatment but not by male sterilization","resumen":"The sterile insect technique (SIT) has been proposed as an area-wide method to control the South American fruit fly, Anastrepha fraterculus (Wiedemann). This technique requires sterilization, a procedure that affects, along with other factors, the ability of males to modulate female sexual receptivity after copulation. Numerous pre-release treatments have been proposed to counteract the detrimental effects of irradiation, rearing and handling and increase SIT effectiveness. These include treating newly emerged males with a juvenile hormone mimic (methoprene) or supplying protein to the male´s diet to accelerate sexual maturation prior to release. Here, we examine how male irradiation, methoprene treatment and protein intake affect remating behavior and the amount of sperm stored in inseminated females. In field cage experiments, we found that irradiated laboratory males were equally able to modulate female remating behavior as fertile wild males. However, females mated with 6-day-old, methoprene-treated males remated more and sooner than females mated with naturally matured males, either sterile or wild. Protein intake by males was not sufficient to overcome reduced ability of methoprene-treated males to induce refractory periods in females as lengthy as those induced by wild and naturally matured males. The amount of sperm stored by females was not affected by male irradiation, methoprene treatment or protein intake. This finding revealed that factors in addition to sperm volume intervene in regulating female receptivity after copulation. Implications for SIT are discussed.","palabras_clave":"SOUTH AMERICAN FRUIT FLY|SIT|METHOPRENE|SPERM STORAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62821,"idioma_id":4,"titulo":"Bayesian Approach to Model CD137 Signaling in Human M. tuberculosis in vitro Responses","resumen":"ct  Immune responses are qualitatively and quantitatively influenced by a complex network of receptor-ligand interactions.  Among them, the CD137:CD137L pathway is known to modulate innate and adaptive human responses against  Mycobacterium tuberculosis. However, the underlying mechanisms of this regulation remain unclear. In this work, we  developed a Bayesian Computational Model (BCM) of in vitro CD137 signaling, devised to fit previously gathered  experimental data. The BCM is fed with the data and the prior distribution of the model parameters and it returns their  posterior distribution and the model evidence, which allows comparing alternative signaling mechanisms. The BCM uses  a coupled system of non-linear differential equations to describe the dynamics of Antigen Presenting Cells, Natural Killer  and T Cells together with the interpheron (IFN)-c and tumor necrosis factor (TNF)-a levels in the media culture. Fast and  complete mixing of the media is assumed. The prior distribution of the parameters that describe the dynamics of the  immunological response was obtained from the literature and theoretical considerations Our BCM applies successively the  Levenberg-Marquardt algorithm to find the maximum a posteriori likelihood (MAP); the Metropolis Markov Chain Monte  Carlo method to approximate the posterior distribution of the parameters and Thermodynamic Integration to calculate the  evidence of alternative hypothesis. Bayes factors provided decisive evidence favoring direct CD137 signaling on T cells.  Moreover, the posterior distribution of the parameters that describe the CD137 signaling showed that the regulation of IFNc levels is based more on T cells survival than on direct induction. Furthermore, the mechanisms that account for the effect  of CD137 signaling on TNF-a production were based on a decrease of TNF-a production by APC and, perhaps, on the  increase in APC apoptosis. BCM proved to be a useful tool to gain insight on the mechanisms of CD137 signaling during  human response against Mycobacterium tuberculosis.","palabras_clave":"CD137 |BAYESIAN|TUBERCULOSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62822,"idioma_id":4,"titulo":"Influence of seasonal variables associated with climate change on photochemical diurnal cycles of marine phytoplankton from Patagonia (Argentina)","resumen":"The effects of temperature and ultraviolet radiation (UVR; 280\u2013400 nm) on seasonal succession in phytoplankton assemblages of Patagonia (Argentina) were studied in the context of global change. Samples collected during pre-bloom, bloom onset, bloom, and spring were exposed to in situ and increased (+4°C) temperatures and solar radiation with and without UVR. Daily cycles of effective photochemical quantum yield exhibited a pattern of high values in the morning, decreasing towards noon, and increasing in the afternoon. The decrease in yields towards noon as the season progressed increased from 30% in the pre-bloom to 80% in the spring; in the latter there were significant differences between radiation treatments under both temperature conditions. The highest inhibition rates were during the bloom, whereas the highest recovery rates were during the spring. Inhibition rates were generally higher in treatments exposed to UVR in comparison to photosynthetically active radiation\u2013only treatments and some stages of the succession exhibited an additional temperature effect. Increasing temperatures had little effect on pre-bloom communities but helped to counteract the magnitude of the yield decrease during the bloom onset. However, during the bloom and in the spring, temperature and UVR acted synergistically, increasing the overall photochemical inhibition. Feedback mechanisms of increased temperatures causing a shallower mixing depth will expose phytoplankton to higher radiation, which will have a negative effect on the bloom and on spring assemblages. Due to the differential effects of solar UVR and increased temperature on phytoplankton, future studies should consider the repercussions on higher trophic levels.","palabras_clave":"TEMPERATURE|ULTRAVIOLET RADIATION|PHYTOPLANKTON ASSEMBLAGES|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62823,"idioma_id":4,"titulo":"Responses of tropical plankton communities from the Mexican Caribbean to solar ultraviolet radiation exposure and increased temperature","resumen":"The aim of this study was to evaluate the effects of UVR on growth and taxonomic composition of tropical plankton communities in a scenario of increased temperature and ultraviolet radiation. Water samples were collected from a reef lagoon in the MexicanCaribbean (20.5° N, 86.5° W) during July 2010 and grown for 16 days in microcosms under two natural radiation treatments: a) PAB (PAR + UV-A + UV-B, 280\u2013700 nm) and, b) P, (PAR, 400\u2013700 nm) and two temperature conditions: a) ambient (28 °C), and, b) increased (ambient + 3 °C). A differential factorial response of the studied variables among the main taxonomic groups and more frequent species was found. The biomass of dinoflagellates and colorless plankton was negatively affected by UVR while the increased temperature had negative effects on diatom biomass and cell abundance. During the experimental period there were changes in the contribution of each taxonomic group. At ambient temperature therewas a shift from aflagellate- to a diatom-dominatedcommunity;whereas at increased temperature diatoms co-dominated withflagellates. UVR exposure decreased the contribution of naked dinoflagellates (&gt;20&#956;m) and cryptophytes. On the other hand, the most frequent diatom,Cylindrotheca closteriumwas negatively affected at increased temperature, while small chlorophytes (b10&#956;m), which were one of the dominant groups offlagellates, contributed significantly to the biomass at increased temperature at the end of the experiment. Synergistic effects of UVR and temperature were only detectedat the species level in largediatoms (&gt;20&#956;m; e.g.Leptocylindrussp. andAmphorasp.) and&nbsp; incryptophytes (&gt;10&#956;m). Our results suggest that planktonic assemblages&nbsp; fromtheMexicanCaribbeanaregenerallywell-adapted to the high UVRfluxes and temperature with some species being positively influenced by increased temperature. However there are exceptions with some species being negatively affected by UVR, increased temperature or the combination of both factors. Therefore, our results indicate that under the high radiation conditions of tropical oceans, changes in community structure in terms of taxonomic composition and size distribution would occur in a scenario of global climate change.","palabras_clave":"UVR|PHYTOPLANKTON"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62824,"idioma_id":4,"titulo":"Interactive effects of vertical mixing, nutrients and ultraviolet radiation: In situ photosynthetic responses of phytoplankton from high mountain lakes of Southern Europe.","resumen":"Global change, together with human activities, has resulted in increasing  amounts of organic material (including nutrients) that water bodies receive.  This input further attenuates the penetration of solar radiation, leading to  the view that opaque lakes are more 'protected' from solar ultraviolet  radiation (UVR) than clear ones. Vertical mixing, however, complicates this  view as cells are exposed to fluctuating radiation regimes, for which the effects  have, in general, been neglected. Furthermore, the combined impacts of mixing,  together with those of UVR and nutrient inputs are virtually unknown. In this  study, we carried out complex in situ experiments in three high mountain  lakes of Spain (Lake Enol in the National Park Picos de Europa, Asturias, and  lakes Las Yeguas and La Caldera in the National Park Sierra Nevada, Granada),  used as model ecosystems to evaluate the joint impact of these climate change  variables. The main goal of this study was to address the question of how  short-term pulses of nutrient inputs, together with vertical mixing and  increased UVR fluxes modify the photosynthetic responses of phytoplankton.  The experimentation consisted in all possible combinations of the following  treatments: (a) solar radiation: UVR + PAR (280-700 nm) versus PAR (photosynthetically active radiation)  alone (400?700 nm); (b) nutrient addition (phosphorus (P) and nitrogen  (N)): ambient versus addition (P to reach to a final concentration of  30 &#956;g P L&#8722;1, and N to reach N:P molar ratio of 31); and (c)  mixing: mixed (one rotation from surface to 3 m depth (speed of  1 m 4 min&#8722;1, total of 10 cycles)) versus static. Our findings suggest  that under ambient nutrient conditions there is a synergistic effect between  vertical mixing and UVR, increasing phytoplankton photosynthetic inhibition  and excretion of organic carbon (EOC) from opaque lakes as compared to algae  that received constant mean irradiance within the epilimnion. The opposite  occurs in clear lakes where antagonistic effects were determined, with mixing  partially counteracting the negative effects of UVR. Nutrient input, mimicking  atmospheric pulses from Saharan dust, reversed this effect and clear lakes  became more inhibited during mixing, while opaque lakes benefited from the  fluctuating irradiance regime. These climate change related scenarios of  nutrient input and increased mixing, would not only affect photosynthesis and  production in lakes, but might also further influence the microbial loop and  trophic interactions via enhanced EOC under fluctuating UVR exposure.","palabras_clave":"UVR|MIXING|NUTRIENTS|LAKES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62825,"idioma_id":4,"titulo":"SPERM MORPHOLOGY OF TWO SPECIES OF OLIVANCILLARIA (GASTROPODA: OLIVIDAE) FROM THE SOUTHWESTERN ATLANTIC","resumen":"            Sperm ultrastructure in two  species of the marine snail family Olividae is examined. Euspermatozoa of both species are  composed of a conical, membrane-bound acrosomal vesicle;  an axial rod and a basal plate similar in both species; a solid and highly electron-dense  nucleus; an elongate midpiece consisting of the axoneme sheathed by helical  mitochondrial elements; an elongate glycogen piece; a double electron-dense  ring at the junction of the midpiece and glycogen piece; and a free tail  region. The slight narrowing in the acrosomal vesicle  invagination is situated in different levels between O. deshayesiana and O.  carcellesi.&nbsp; This morphology could be  considered as specific character. The length of the  nucleus in Olivancillaria carcellesi  and Olivancillaria deshayesiana is shorter  than that of other neogastropods, and could be diagnostic at family  level.","palabras_clave":"olividae|sperm ultrastructure|reproduction|south atlantic ocean"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62826,"idioma_id":4,"titulo":"Rapid evolution of male genitalia among populations of Drosophila buzzatii.","resumen":"Increasing evidence from multiple animal systems suggests that genital evolution and diversification are driven by rapid and strong evolutionary forces. Particularly, the morphology of male genital structures is considered to be among the fastest evolving traits in animal groups with internal fertilization. In this study, we investigated patterns of male genital variation within and between natural populations of the cactophilic fly Drosophila buzzatii in its original geographic distribution range in the Neotropics. We detected significant morphological differences among populations and distinguished five differentiated groups. Moreover, among population differentiation in genital morphology was associated with the degree of geographic isolation among populations and clearly contrasted with the general homogeneity detected for the putatively neutral mitochondrial gene COI. Integrating our present data with previous molecular population genetic surveys, our results suggest that male genital morphology has rapidly diverged after the recent demographic expansion that D. buzzatii has undergone in the arid zones of South America. Because the ?lock and key? hypothesis failed to explain the present pattern, we explored alternative explanations for the observed pattern of genital diversification including drift-facilitated sexual selection.","palabras_clave":"aedeagus|Chromosomal inversion|morphological evolution|FST"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62827,"idioma_id":4,"titulo":"Phosphorylation of Mitogen-Activated Protein Kinases Contributes to Interferon &#947; Production in Response to Mycobacterium tuberculosis","resumen":"Immune control of Mycobacterium tuberculosis depends on interferon &#947; (IFN-&#947;)-producing CD4(+) lymphocytes. Previous studies have shown that T cells from patients with tuberculosis produce less IFN-&#947;, compared with healthy donors, in response to mycobacterial antigens, although IFN-&#947; responses to mitogens are preserved. In this work, we found that M. tuberculosis-induced IFN-&#947; production by human T cells correlated with phosphorylation of the mitogen-activated protein kinases (MAPKs), extracellular signal-regulated kinase (ERK), and p38. Moreover, the majority of IFN-&#947;-producing T cells expressed signaling lymphocyte activation molecule (SLAM), and SLAM activation further increased ERK phosphorylation. Interestingly, patients with tuberculosis had delayed activation of ERK and p38, and this was most marked in patients with the poorest IFN-&#947; responses (ie, low responders). Besides, SLAM signaling failed to phosphorylate ERK in low responders. Our findings suggest that activation of p38 and ERK, in part through SLAM, mediates T-cell IFN-&#947; production in response to M. tuberculosis, a pathway that is defective in patients with tuberculosis.","palabras_clave":"TUBERCULOSIS|SIGNALING|IFNg"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62828,"idioma_id":4,"titulo":"Indium-Mediated Regioselective Synthesis of Ketones from Arylstannanes under Solvent-Free Ultrasound Irradiation","resumen":"The solvent-free indium-promoted reaction of alkanoyl chlorides with sterically and electronically diverse arylstannanes is a simple and direct method for the regioselective synthesis of primary, secondary and tertiary alkyl aryl ketones in good to excellent isolated yields (42-84%) under mild and neutral conditions. The protocol is also adequate for the synthesis of aryl vinyl ketones. Reaction times are drastically reduced (from 3-32 hs to 10-70 min) under ultrasonic irradiation. Evidences for the involvement of a homolytic aromatic ipso-substitution mechanism, in which indium metal acts as radical initiator, are presented. It is possible the transference of two aryl groups from tin, thus improving effective mass yield, working with diarylstannanes as starting substrates.","palabras_clave":"Ultrasound|Solvent-free|Aromatic ketones|Arylstannanes|Indium"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62829,"idioma_id":4,"titulo":"Development and validation of a novel diagnostic test for human brucellosis using a glyco-engineered antigen coupled to magnetic beads","resumen":"Brucellosis is a highly contagious zoonosis and still a major human health problem in endemic areas of the world. Although several diagnostic tools are available, most of them are difficult to implement especially in developing countries where complex health facilities are limited. Taking advantage of the identical structure and composition of the Brucella spp. and Yersinia enterocolitica O:9 O-polysaccharide, we explored the application of a recombinant Y. enterocolitica O:9-polysaccharide-protein conjugate (OAg-AcrA) as a novel antigen for diagnosis of human brucellosis. We have developed and validated an indirect immunoassay using OAg-AcrA coupled to magnetic beads. OAg-AcrA was produced and purified with high yields in Y. enterocolitica O:9 cells co-expressing the oligosaccharyltransferase PglB and the protein acceptor AcrA of Campylobacter jejuni without the need for culturing Brucella. Expression of PglB and AcrA in Y. enterocolitica resulted in the transfer of the host O-polysaccharide from its lipid carrier to AcrA. To validate the assay and determine the cutoff values, a receiver-operating characteristic analysis was performed using a panel of characterized serum samples obtained from healthy individuals and patients of different clinical groups. Our results indicate that, using this assay, it is possible to detect infection caused by the three main human brucellosis agents (B. abortus, B. melitensis and B. suis) and select different cutoff points to adjust sensitivity and specificity levels as needed. A cutoff value of 13.20% gave a sensitivity of 100% and a specificity of 98.57%, and a cutoff value of 16.15% resulted in a test sensitivity and specificity of 93.48% and 100%, respectively. The high diagnostic accuracy, low cost, reduced assay time and simplicity of this new glycoconjugate-magnetic beads assay makes it an attractive diagnostic tool for using not only in clinics and brucellosis reference laboratories but also in locations with limited laboratory infrastructure and\/or minimally trained community health workers","palabras_clave":"serological diagnosis|human brucellosis|glycoconjugated|nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62830,"idioma_id":4,"titulo":"A complete O(alpha_S^2) calculation of the signal-background interference for the Higgs diphoton decay channel","resumen":"We present the full $O(alpha^2_S)$ computation of the interference effects between the Higgs diphoton signal and the continuum background at the LHC. While the main contribution to the interference originates on the gg partonic subprocess, we find that the corrections from the qg and q-qbar channels amount up to 35 % of it. We discuss the effect of these new subprocesses in the shift of the diphoton invariant mass peak recently reported by S. Martin in (Phys. Rev. D 86:073016, 2012).","palabras_clave":"Higgs|diphoton channel|QCD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62831,"idioma_id":4,"titulo":"PREDICTING GREEN LEAF PROPORTION IN UNGRAZED KLEINGRASS (Panicum coloratum L.) IN THE SEMIARID PAMPAS REGION OF ARGENTINA","resumen":"The  aims of this work were to establish whether a stable relationship exists  between the proportion of green leaf blade was carried out during four consecutive growing  seasons in an established pasture of kleingrass. During two growing seasons, 90 kg ha-1 of N was applied. The forage samples were at biweekly intervals and separated into leaf blade (inclusive of leaf sheaths) and senescent material. Regression  analysis indicated a  relationship (y = 0.70x-0.41, R = 0.84, P < 0.001, n = 180) between the proportion of green leaf blade and total mass of harvestable forage in ungrazed kleingrass, even under varying environmental conditions of  rainfall and nitrogen availability. Thus, total harvestable forage might be  used to predict the green leaf proportion of kleingrass in contrasting  environments, and used as an easy measure indicator of green leaf proportion that would permit a balance of forage quality and quantity in kleingrass.","palabras_clave":"GREEN LEAF|LEAF:STEM RATIO|TOTAL HARVESTABLE FORAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62832,"idioma_id":4,"titulo":"Crustal structure of the Central Precordillera of San Juan, Argentina(31°S) using teleseismic receiver functions","resumen":"The subduction of the Nazca plate under the South American plate around 31S is characterized by flat slab geometry. The (Chilean) Pampean flat slab of Argentina associated with the subduction of the Juan Fernandez ridge lies in a region of a series of foreland uplifts corresponding to the thin-skinned Pre- cordillera and basement cored Sierras Pampeanas ranges. The SIEMBRA project deployed 40 broadband stations in 2008e2009 in both the Precordillera and the Sierras Pampeanas with the aim to foster the understanding of the entire central Andean flat slab region. One of the SIEMBRA station (DOCA) located on the western flank of Sierra de la Invernada in the Central Precordillera appears particularly appro- priate to study the crustal structure and eventually detect discontinuities related to terranes establish- ment. We thus performed a receiver function analysis using teleseismic data recorded at the DOCA station during the SIEMBRA project and from October 2011 to June 2012 using a broadband UNSJ (Na- tional University of San Juan) seismic station with the purpose to obtain crustal images with details of the intracrustal structure consistent with a mechanism that could explains both the observed earthquake depths and the uplift pattern in the Central Precordillera. Our results show that the Moho beneath the Precordillera lies at a depth of about 66 km. The Moho signal appears diminished and behaves irregularly as a function of azimuthal orientations. Although this observation could be the result of an irregular geometry it also correlates with the hypothesis of partial eclogitisation in the lower crust. Two mid- crustal discontinuities have also been revealed. The shallower one could correspond to a décollement level between the Precordilleran strata and the Cuyania basement at 21 km depth. The deeper one which the presence has been matched with a sharp decrease of the crustal seismic activity drove us to the hypothesis of a major change in crustal composition at 36 km. Finally the flat portion of the subducted slab has been imaged lying at about 100 km depth.","palabras_clave":"Andean retroarc|Flat slab|Décollement|Partial eclotigitization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62833,"idioma_id":4,"titulo":"Field preferences of the Social Wasp Vespula germanica (Hymenoptera: Vespidae) for Protein-rich Baits","resumen":"Food preferences displayed by foraging insects are important from a fundamental perspective and in pest control. We studied the preference of an invasive wasp, V. germanica, for protein foods in field conditions. Preferences were evaluated by placing baits in a paired design in different habitats and analyzing wasp visits, using a Bayesian approach to the Thurstone model. V. germanica workers display a clear rating of preferences, but were affected by the presence of competitors at the bait. These results contribute knowledge aimed at toxic baiting protocols for this wasp and suggest that food choice is a complex process subject to the influence of diverse factors. We emphasize the importance of on-site paired comparisons in preference studies to fully understand the drivers of food choice by insects.","palabras_clave":"invasive insects|Patagonia|Yellowjackets|social insect management"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62834,"idioma_id":4,"titulo":"Plant growth and survival of five perennial grass genotypes exposed to various defoliation managements in arid Argentina","resumen":"The field performance of the native Pappophorum vaginatum, the naturalizedEragrostis curvula, and various cultivars of the introduced Achnatherum hymenoides and Leymus cinereus were evaluated as potential forage resources in rangelands of arid Argentina during the warm-seasons of 2007\/2008 and 2008\/2009. Plants of these grass species, obtained from seeds, were transplanted to the field in 2006, when they were one-year-old. During the study years, there were two defoliation managements: plants of all study genotypes either remained undefoliated (controls) or were defoliated twice a year during spring at 5 cm stubble height. Despite tiller number being lower (p0.05) between defoliation managements by mid-spring, there were no ifferences (p>0.05) in dry weight production between defoliated and ndefoliatedplants in all genotypes at the end of the second growing season. Plants of one or more of the introduced genotypes showed a similar (p>0.05) or greater p0.05) or greater (p 70%) ornaturalized (> 39%) genotypes at the end of the first or second growing seasons.","palabras_clave":"plant demography|plant growth|perennial grass genotypes|arid Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62835,"idioma_id":4,"titulo":"Early maternal separation: neurobehavioral consequences in mother rats","resumen":"Repeated separation of dams from their pups during the postpartum period may evoke emotional stress in the dam. In the present study we investigated whether prolonged maternal separation is stressful for rat dams by studying different behavioral and central responses known to be affected by stress. After delivery, female Wistar rats were subjected to either animal facility rearing (AFR) conditions or daily 4.5 h of mother?litter separation from postpartum day (PPD) 1?21. Maternal care (pup retrieval) was evaluated at PPD 3. After weaning on PPD 21, anxiety (elevated plus maze) and depression-like behaviors (forced swimming test) were assessed in the dams. Memory abilities (one-trial step down inhibitory avoidance) were tested either 1 h (short-term memory) or 24 h (long-term memory) after training session. Finally, c-Fos expression was examined in the central nucleus of the amygdala. The results revealed that pup retrieval efficiency at PPD 3 was significantly impaired by maternal separation. AFR dams retrieved their pups sooner and engaged in more pup-directed activities (nest building and carrying pups). Separation from pups increased the number of entries in open arms of the plus maze and decreased latency times in the inhibitory avoidance test for both short and long-term memory in the dams. There were no differences in depression-related behavior as assessed using the forced swimming test. Furthermore, maternal separation yielded high c-Fos expression in the central nucleus of the amygdala. Together, these data indicate that repeated maternal separation in the early postpartum period reduces maternal care and impairs the retention memory, providing further evidence for the detrimental neurobehavioral effects of maternal separation in dams. ","palabras_clave":"Maternal separation|Maternal behavior|Anxiety|Depression"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62836,"idioma_id":5,"titulo":"Optimization and Evaluation of an Arterial  Thrombus Retrieval Device","resumen":"The aim of this work was the optimization of anarterial clot retrieval device made of Ni-Ti in the shape of ahelical spring. Wire was obteined from GAC International Inc., acompany dedicated to the development of products vinculated toorthodontic and orthopedic, and then employed for itsdevelopment. Finite Element (FE) simulation was used tocompare the effects in the clot when different shapes of springs,cylindrical and conical, are used. Abaqus 6.9 was the softwarechosen to develop those simulations. The assessment of the deviceusing an in vivo model was attempted; an experimental protocolwas developed, using the abdominal aorta of the rat for effectivein vivo testing of the endovascular catheter-delivered device.Taking into account the results obtained with the FE analysis itwas concluded that the utilization of conical shaped springs isbetter than cilindrical due to the lower stress caused in the clot inthis case, that avoids its rupture. The in vivo intervention wassuccesfull, the generation of the clot in the aorthic artery of a ratwas achieved and so was the extraction of it.","palabras_clave":"MECHANICAL EMBOLECTOMY |NITINOL|FINITE ELEMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62837,"idioma_id":4,"titulo":"Neurobiological effects of neonatal maternal separation and post-weaning environmental enrichment","resumen":"Throughout the lifespan, the brain has a considerable degree of plasticity and can be strongly influenced bysensory input from the outside environment. Given the importance of the environment in the regulationof the brain structure, behavior and physiology, the aim of the present work was to analyze the effectsof different environmental qualities during two critical ontogenic periods (early life and peripuberty) onbehavior and hippocampal physiology.Male Wistar rats were separated from their mothers for 4.5 h daily during the first 3 weeks of life.They were weaned on day 21 and housed under either standard or enriched conditions. At 60 d of age,all animals were then housed in same-treatment groups, two per cage, until testing began on day 74.Emotional and cognitive responses were tested using the open field, novel object recognition test andstep-down inhibitory avoidance learning. In the dorsal hippocampus, glucocorticoid receptor expressionand neuronal activity were examined by immunoreactivity.Grooming behavior in the open field was found to be significantly lower in maternally separatedanimals, but post-weaning environmental enrichment completely reversed this tendency. Inhibitoryavoidance but not object recognition memory was impaired in maternally separated animals, suggestingthat early maternal separation alters learning and memory in a task-specific manner. Again, environmentalenrichment reversed the effects of maternal separation on the inhibitory avoidance task. Eventhough maternal separation did not significantly affect Fos and glucocorticoid receptor (GR) expression,environmental enrichment increased both Fos expression in the total hippocampal area and also theoverall number of GR positive cells per hippocampal area, mainly due to the changes in CA1.These findings suggest that differential rearing is a useful procedure to study behavioral and physiologicalplasticity in response to early experience and that, although the effects of adverse experienceearly in life such as maternal separation can persist until adulthood, some of them can be compensatedby early favorable environments, possibly through nervous system plasticity.","palabras_clave":"MATERNAL SEPARATION|ENVIRONMENTAL ENRICHMENT|HIPPOCAMPUS|MEMORY|GLUCOCORTICOID"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62838,"idioma_id":4,"titulo":"Mifepristone, a Blocker of Glucocorticoid Receptors, Promotes Photoreceptor Death","resumen":"Purpose. Glucocorticoids are best known by their protective effect on retinal photoreceptor damage. However, they could also be involved in photoreceptor homeostasis under basal, nonstressful conditions. Therefore, we aimed to study glucocorticoid-induced changes of survival-related molecules in male mice retinas under standard illumination conditions (12 hours light, &#8804; 60 lux\/12 h dark).Methods. Male Balb-c mice were injected with dexamethasone (DEX), a selective glucocorticoid receptor &#945; (GR&#945;) agonist, its antagonist mifepristone (MFP), or both drugs (D+M) at noon. A group of mice was subjected to surgical adrenalectomy (AdrX). Retinas were studied by histology, immunohistochemistry, TUNEL procedure, and Western blotting at different periods after pharmacological or surgical intervention (6 hours, 48 hours, or 7 days).Results. The antiapoptotic molecule Bcl-XL significantly increased 6 hours after DEX injection. By contrast, this molecule could no longer be found after MFP injection. At the same time, high levels of cleaved caspase-3 (CC-3) and Bax appeared in retinal extracts, and TUNEL+ nuclei selectively showed in the outer nuclear layer (ONL). After MFP, retinal extracts also contained phosphorylated histone H2AX (p-H2AX), a marker of DNA breakage and repair. Loss of ONL nuclear rows and decrease of rhodopsin levels were evident 7 days after MFP administration. These changes were minimized when DEX was given together with MFP (D+M). In the absence of MFP, DEX increased Bcl-XL in every retinal layer, with a marked intensification in photoreceptor inner segments. Numerous TUNEL+ nuclei rapidly appeared in the ONL after AdrX.Conclusions. A single dose of MFP induced selective photoreceptor damage in the absence of other environmental stressors. Because damage was prevented by DEX, and was reproduced by AdrX, our findings suggest that glucocorticoids play a critical role in photoreceptor survival.","palabras_clave":"MIFEPRISTONE|GLUCOCORTICOID RECEPTOR|PHOTORRECEPTOR DEATH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62839,"idioma_id":4,"titulo":"A NEW REFINEMENT WAVELET&GALERKIN METHOD IN A SPLINE LOCAL MULTIRESOLUTION ANALYSIS SCHEME FOR BOUNDARY VALUE PROBLEMS","resumen":"In solving differential equations, wavelets provide a robust and accurate alternative  to traditional methods and their advantage is really appreciated when they  are applied to problems having localized singular behavior. The solution is approximated  by an expansion of scaling functions and wavelets, with the convenience that  multiscale and localization properties can be exploited. The choice of wavelet basis  is governed by several factors including the desired order of numerical accuracy and  computational effort.  A good feature of wavelet methods is the possibility to apply adaptive techniques.  In some cases multiscale bases are combined with finite element methods","palabras_clave":"Multiresolution Analisis|Spline Function|Wavelet- Galerkin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62840,"idioma_id":4,"titulo":"ALTERNATIVE LOW-COST APPROACH TO THE SYNTHESIS OF MAGNETIC IRON OXIDE NANOPARTICLES BY THERMAL DECOMPOSITION OF ORGANIC PRECURSORS","resumen":"AbstractA new approach to thermal decomposition of organic iron precursors is reported, which results in a simpler and more economical method to produce well crystallized&nbsp; gamma-Fe2O3 nanoparticles (NPs) with average sizes within the 3\u201317 nm range. The NPs were characterized by TEM, SAED, XRD, DLS-QELS, M¨ossbauer spectroscopy at different temperatures, FT-IR and magnetic measurements. The obtained&nbsp; gamma-Fe2O3 NPs are coated with oleic acid and, in a lower quantity, with oleylamine (about 1.5 nm in thickness). It was shown that changing operative variables allows us to tune the average particle diameters and obtain a very narrow or monodisperse distribution of sizes. The &nbsp;-Fe2O3 NPs behave superparamagnetically at room temperature and their magnetization saturation is reduced by about 34% in comparison with bulk maghemite. The results indicate that the distance between two neighbour NPs, generated by the coating, of about 3 nm is insufficient to inhibit interparticle magnetic interactions when the average diameter is 8.8 nm. The good quality of the NPs, obtained through the present low-cost and easy-handling process, open a new perspective for future technological applications.","palabras_clave":"Maghemite nanoparticles synthesis|Mössbauer spectroscopy|Dipolar magnetic interactions|Magnetic measurements"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62841,"idioma_id":4,"titulo":"Fusarium cerealis associated with barley seeds in Argentina","resumen":"Fusarium Head Blight is a fungal disease caused by a complex of Fusarium species on cereals, such as barley and wheat. It has economic impacts due to yield reductions and mycotoxin contamination. As barley production has increased considerably in the last 5 years in Argentina a survey was conducted for identifying Fusarium species associated with barley grains. Fusarium cerealis was isolated and identified based on morphological and molecular analysis. The potential production of nivalenol and zearalenone was assessed using specific PCR assays. Koch´s postulates were carried out to confirm the pathogenicity of the fungus.","palabras_clave":"FUSARIUM CEREALIS|HORDEUM VULGARE|NIVALENOL|ZEARALENONE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62842,"idioma_id":4,"titulo":"Self-Assembled Monolayers of NH2-Terminated Thiolates: Order, pKa and Specific Adsorption","resumen":"Self-assembled monolayers (SAMs) of amino-terminated alkanethiols on Au were characterized by a combination of electrochemical (LSV, CV and EIS) and spectroscopic (XPS and SER) techniques. Clear correlations were obtained between the apparent surface pKa values determined by impedimetric titrations and order parameters such as the content of trans conformers in the SAMs. These results contrast with previous studies that exhibit dispersions of up to 6 pH units in the reported pKa values. In addition, we determined that inorganic and organic phosphate species bind specifically to these SAMs mediating adsorption and heterogeneous electron transfer of positively charged macromolecules such as cytochrome c.","palabras_clave":"Adsorption| Surface Properties|Sulfhydryl Compounds"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62843,"idioma_id":4,"titulo":"Transcriptional regulation of Insulin Degrading Enzyme modulates mitochondrial A&#946; catabolism and functionality","resumen":"Studies of postmortem brains from Alzheimer?s disease (AD) patients suggest that oxidative damage induced by mitochondrial A&#946; (mitA&#946;) accumulation is associated with mitochondrial dysfunction. However, the regulation of mitA&#946; metabolism is unknown. One of the proteases involved in mitA&#946; catabolism is long Insulin-Degrading Enzyme (IDE) isoform (IDE-Met1). Yet, the mechanisms of its expression are unknown and its presence in brain uncertain. We detected IDE-Met1 in brain and showed that its  expression is regulated by mitochondrial biogenesis pathway (PGC-1&#945;\/NRF-1). A strong positive correlation between PGC-1&#945; or NRF-1 and L-IDE transcripts was found in nondemented brains. This correlation was weaker  in AD. In vitro inhibition of IDE increased mitA&#946; and impaired mitochondrial respiration.  These changes were restored by inhibition of &#61543;-  secretase or promotion of mitochondrial  biogenesis. Our results suggest that IDE-Met1  links mitochondrial biogenesis pathway with  mitA&#946; levels and organella functionality","palabras_clave":"FACTOR DE TRANSCRIPCION|METABOLISMO MITOCONDRIAL|AMILOIDES|NEURODEGENERACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62844,"idioma_id":4,"titulo":"Spectroscopic characterization of mixed cation diphosphates of the type M(I)Fe(III)P2O7 (with M(I) = Li,Na,K,Rb,Cs,Ag)","resumen":"The mixed cation diphosphates of composition M(I)Fe(III)P2O7 have been prepared using the so called co-precipitation method starting with Fe(III) and M(I) nitrate solutions and solid (NH4)H2PO4, and characterized by X-ray powder diffractometry. The infrared and Raman spectra of the compounds were recorded and the results are briefly discussed on the basis of their structural peculiarities.The 57Fe-Mössbauer spectra show that the typical high-spin Fe(III)O6 octahedra existent in these compounds exhibit almost no distortion. However, the hyperfine parameters are sensitive to some characteristics of the M(I) cations and to the different structural types.&nbsp;","palabras_clave":"Inorganic compounds|Chemical synthesis|IR and Raman spectroscopy|Mössbauer spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62845,"idioma_id":4,"titulo":"New Findings for the Composition and Structure of Ni Nanoparticles Protected with Organomercaptan Molecules","resumen":"Here we explore the synthesis of alkanethiolcoatedNi NPs following the one-phase reaction method byBrust et al.1 The reduction of NiCl2 with NaBH4 in thepresence of dodecanethiol (C12SH) yields a complex productthat is difficult to identify as illustrated in the figure of merit.We synthesized Ni(II) dodecanethiolate (C12S) (without theaddition of NaBH4) for comparison and performed anexhaustive characterization with TEM, HR-TEM, AFM,MFM, XPS, XRD, UV&#8722;vis, magnetism, and FT-IR. It isfound that the organic coating is not quite a well-organized self-assembled monolayer (SAM) surrounding the Ni cluster aspreviously reported.2,3 XPS and XRD data show slight differences between both syntheses; however, Ni(II) thiolate appears to bemore stable than reduced Ni when exposed to ambient air, indicating the propensity of metallic Ni to oxidize. It has been shownthat irradiating with TEM electrons over various metal thiolates leads to nanoparticle formation.4 We irradiated over Ni(II)thiolate and observed no evidence of NP formation whereas irradiating a reduced Ni sample exhibited an &#8764;3.0 nm nanoparticlediameter. Magnetism studies showed a difference between both samples, indicating ferromagnetic character for the reduced Nisample. According to our results, the product of the synthesis is comprised of ultrasmall metallic clusters embedded in some formof Ni(II) C12S. In this work, we open a discussion of the chemical nature of the core and the shell in the synthesis of Ni NPsprotected with organomercaptan molecules.","palabras_clave":"Ni nanoparticles |XPS|TEM|AFM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62846,"idioma_id":4,"titulo":"Transient expression of VP2 in Nicotiana benthamiana and its use as a plant-based vaccine  against Infectious Bursal Disease Virus","resumen":"Infectious Bursal Disease Virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds. This disease causes important economic losses in the poultry industry worldwide. The VP2 protein has been used for the development of subunit vaccines in a variety of heterologous platforms. In this context, the aim of this study was to investigate VP2 expression and immunogenicity using an experimental plant-based vaccine against IBDV. We determined that the agroinfiltration of N. benthamiana leaves allowed the production of VP2 with no apparent change on its conformational epitopes. Chickens intramuscularly immunized in a dose\/boost scheme with crude concentrated extracts developed a specific humoral response with viral neutralizing ability. Given these results, it seems plausible for a plant-based vaccine to have a niche in the veterinary field. Thus, plants can be an adequate system of choice to produce immunogens against IBDV.","palabras_clave":"RECOMBINANT VACCINES|PLANT EXPRESSION|VETERINARY ANTIGENS|IMMUNE RESPONSE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62847,"idioma_id":4,"titulo":"Search for dark matter candidates and large extra dimensions in events with a jet and missing transverse momentum with the ATLAS detector","resumen":"A search for new phenomena in events with a high-energy jet and large missing transverse momentum is performed using data from proton-proton collisions at                                 s&#8730;=7                               TeV with the ATLAS experiment at the Large Hadron Collider. Four kinematic regions are explored using a dataset corresponding to an integrated luminosity of 4.7 fb&#8722;1. No excess of events beyond expectations from Standard Model processes is observed, and limits are set on large extra dimensions and the pair production of dark matter particles.","palabras_clave":"dark|matter|search|ATLAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62848,"idioma_id":4,"titulo":"Biodistribution of sodium borocaptate (BSH) for boron neutron capture therapy (BNCT) in an oral cancer model","resumen":"Abstract:      Boron neutron capture therapy (BNCT) is based on selective accumulation of 10B carriers in tumor followed by neutron irradiation. We previously proved the therapeutic success of BNCT mediated by the boron compounds boronophenylalanine and sodium decahydrodecaborate (GB-10) in the hamster cheek pouch oral cancer model. Based on the clinical relevance of the boron carrier sodium borocaptate (BSH) and the knowledge that the most effective way to optimize BNCT is to improve tumor boron targeting, the specific aim of this study was to perform biodistribution studies of BSH in the hamster cheek pouch oral cancer model and evaluate the feasibility of BNCT mediated by BSH at nuclear reactor RA-3. The general aim of these studies is to contribute to the knowledge of BNCT radiobiology and optimize BNCT for head and neck cancer. Sodium borocaptate (50 mg 10B\/kg) was administered to tumor-bearing hamsters. Groups of 3-5 animals were killed humanely at nine time-points, 3,12 h post-administration. Samples of blood, tumor, precancerous pouch tissue, normal pouch tissue and other clinically relevant normal tissues were processed for boron measurement by optic emission spectroscopy. Tumor boron concentration peaked to therapeutically useful boron concentration values of 24,35 ppm. The boron concentration ratio tumor\/normal pouch tissue ranged from 1.1 to 1.8. Pharmacokinetic curves showed that the optimum interval between BSH administration and neutron irradiation was 7-11 h. It is concluded that BNCT mediated by BSH at nuclear reactor RA-3 would be feasible.","palabras_clave":"BORON NEUTRON CAPTURE THERAPY|BNCT|ORAL CANCER|HAMSTER CHEEK POUCH|PRECANCEROUS TISSUE|BSH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62849,"idioma_id":4,"titulo":"New genetic maps for globe artichoke and wild cardoon and their alignment with an SSR-based consensus map","resumen":"An F1 mapping population was bred by crossing an accession of wild cardoon with a single Argentinian globe artichoke plant of the variety Estrella del Sur FCA with a view to generating new Cynara cardunculus linkage maps. Genotyping was conducting using a set of 553 SRAP, SSR, AFLP and SNP markers. The 1,465.5 cM map based on the segregation of alleles present in the wild cardoon parent comprised 214 loci distributed across 16 linkage groups (LGs), while the 910.1 cM globe artichoke-based map featured 141 loci falling into 12 LGs covering the total length. Three of the morphological traits (head spininess, leaf spininess and head color) for which the parents contrasted were inherited monogenically, and the genes conditioning them were mapped. A set of 48 co-dominant loci was used to align the LGs with those derived from a reference SSR-based consensus map of the species.","palabras_clave":"Cynara cardunculus  \u0002  \u0002|Linkage groups|\u0002 Molecular markers| Genetic map"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62850,"idioma_id":4,"titulo":"Combining abilities and Heterotic group in Pisum sativum L","resumen":"The objective of this research was to investigate the use of heterosis  and estimates of the general combining ability (GCA) and specific combining ability (SCA) for yield and the yield-related traits for identifying suitable parents and forming heterotic groups for pea- breeding programs. Seventy-six F1 hybrids derived from crosses between nineteen female lines and four male testers were evaluated during two seasons. Estimates of variance due to GCA and SCA effects and their relationship revealed predominantly additive effects for all traits. Parents with higher GCA values were ?ZAV20? (female parent) and ?ZAV23? (male parent).The cross ?ZAV5 x ZAV23? showed the highest value for seed yield. Days to flowering and number of seeds per plot were the variables with the highest values for broad and narrow-sense heritability (0.93 and 0.65, respectively), indicating that these traits are highly heritable. The highest best parent heterosis for seed yield was observed in the ?ZAV17 x DDR14? hybrid. Four heterotic groups were formed and validated by estimating the intra and inter group heterosis.","palabras_clave":"Pisum sativum L.|LINE X TESTER|SEED YIELD|COMBINING ABILITY|HETEROSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62851,"idioma_id":4,"titulo":"Mitochondrial dysfunction and therapeutic approaches in respiratory and limb muscles of cancer cachectic mice","resumen":"Abnormalities in mitochondrial content, morphology, and function were reported in several muscle wasting conditions. We specifically explored whether experimental cancer-induced cachexia may alter mitochondrial respiratory chain (MRC) complexes and oxygen uptake in respiratory and peripheral muscles, and whether signaling pathways, proteasome, and oxidative stress may influence that process. We evaluated complex I, II, and IV enzyme activities (specific activity assays) and MRC oxygen consumption (polarographic measurements) in diaphragm and gastrocnemius of cachectic mice bearing the LP07 lung tumor with and without treatment with N-acetylcysteine, bortezomib, and nuclear factor (NF)-kB (sulfasalazine) and mitogen-activated protein kinases (MAPK, U0126) inhibitors, n=10\/group, all groups. Whole body and muscle weights and limb muscle force were also assessed in all rodents at baseline and after one month. Compared to controls, cancer cachectic mice showed a significant reduction in body weight gain, smaller sizes of diaphragm and gastrocnemius, lower muscle strength, and decreased activity of complexes I, II, and IV, and oxygen consumption in both muscles. Blockade of NF-kB and MAPK actions restored muscle mass loss and force, and MRC dysfunction in both muscles, while partly reducing tumor burden. Antioxidants improved mitochondrial oxygen uptake without eliciting significant effects on muscle mass loss and force or tumor size, whereas the proteasome inhibitor reduced tumor burden without significantly influencing muscle mass and strength or mitochondrial function. In conclusion, NF-kb and MAPK signaling pathways modulate muscle mass and performance and MRC function of respiratory and limb muscles in this model of experimental cancer cachexia, thus offering targets for therapeutic intervention.","palabras_clave":"LUNG CANCER CACHEXIA|MITOCHONDRIAL RESPIRATORY CHAIN|OXYGEN CONSUMPTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62852,"idioma_id":4,"titulo":"Therapeutic effects of Salmonella typhi in a mouse model of T-cell  lymphoma.","resumen":"In this study we assessed the effectiveness of a live, attenuated Salmonella enterica serovar Typhi vaccine strain as a cancer immunotherapy in a mouse model of metastatic T-cell lymphoma. EL4 tumor-bearing C57BL\/6J mice immunized with Salmonella Typhi strain CVD 915, by injection into the tumor and the draining lymph node areas, displayed a significant decrease in tumor growth, a reduction in the mitotic index of tumors, a delayed development of palpable lymph node metastases and most importantly improved survival, compared to untreated mice. Besides, complete tumor regression was achieved in a small number of bacteria-treated mice. A successful therapeutic response associated with a significant reduction of tumor mass was evident as early as 5 days after treatment. The administration of Salmonella to tumorbearing mice promoted early cellular infiltration (mainly neutrophils) within the tumor, and was accompanied by a decreased intratumoral IL-10 production as well as by leukocyte expansion in tumor draining lymph nodes. A tumor-specific memory immune response was induced in most of cured animals, as evidenced by the lack of tumor growth after a rechallenge with the same tumor. EL4 cultured with live Salmonella failed to proliferate and underwent apoptosis in a dose-, time-, and contactdependent manner. To our knowledge, these results demonstrate for the first time the efficacy of a Salmonella Typhi vaccine strain as an oncolytic and immunotherapeutic agent against a highly malignant tumor and support the use of Salmonella Typhi-based immunotherapy.","palabras_clave":"cancer immunotherapy|Salmonella Typhi|antitumor efficacy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62853,"idioma_id":4,"titulo":"Prenatal stress increases the expression of proinflammatory cytokines and exacerbates the inflammatory response to LPS in the hippocampal formation of adult male mice","resumen":"Early life experiences, such as prenatal stress, may result in permanent alterations in the function of the nervous and immune systems. In this study we have assessed whether prenatal stress affects the inflammatory response of the hippocampal formation of male mice to an inflammatory challenge during adulthood. Pregnant C57BL\/6 mice were randomly assigned to stress (n=10) or non-stress (n=10) groups. Animals of the stress group were placed in plastic transparent cylinders and exposed to bright light for 3 sessions of 45min every day from gestational day 12 to parturition. Non-stressed pregnant mice were left undisturbed. At four months of age, non stressed and prenatally stressed male offspring were killed, 24h after the systemic administration of lipopolysaccharide (LPS) or vehicle. Under basal conditions, prenatally stressed animals showed increased expression of interleukin 1&#946; and tumor necrosis factor-&#945; (TNF-&#945;) in the hippocampus and an increased percentage of microglia cells with reactive morphology in CA1 compared to non-stressed males. Furthermore, prenatally stressed mice showed increased TNF-&#945; immunoreactivity in CA1 and increased number of Iba-1 immunoreactive microglia and GFAP-immunoreactive astrocytes in the dentate gyrus after LPS administration. In contrast, LPS did not induce such changes in non-stressed animals. These findings indicate that prenatal stress induces a basal proinflammatory status in the hippocampal formation during adulthood that results in an enhanced activation of microglia and astrocytes in response to a proinflammatory insult.","palabras_clave":"Prenatal stress|inflammation|cytokines"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62854,"idioma_id":4,"titulo":"Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins","resumen":"In oviparous species, proteins and lipids found in the vitellus form the lipoproteins called lipovitellins that arethe major source of energy for the development, growth, and survival of the embryo. The energy resourcesprovided by the lipovitellins have not yet been investigated in the Order Araneae. Using the wolf spiderSchizocosa malitiosa (Lycosidae) as an experimental model, we identified and characterized the lipovitellinspresent in the cytosol, focusing on the energetic contribution of those lipoprotein particles in the vitellus.Two lipovitellins (LV) named SmLV1 and SmLV2 were isolated. SmLV1 is a high-density lipoproteinwith 67% lipid and 3.6% carbohydrate, and SmLV2 is a very high-density lipoprotein with 9% lipid and 8.8%carbohydrate. Through electrophoresis in native conditions we observed that SmLV1 has a molecular massof 559 kDa composed of three apolipoproteins of 116, 87, and 42 kDa, respectively. SmLV2 comprised severalproteins composed of different proportions of the same subunits (135, 126, 109, and 70 kDa). The principallipids of these lipovitellins are sphingomyelin + lysophosphatidylcholine, esterified sterols, and phosphatidylcholine.Lipovitellin-free cytosol contains abundant phospatidylcholine and triacylglyceride relatedto the yolk nuclei (the vitellus organizing center). The principal fatty acids of SmLV1 and SmLV2 are 18:2n&#8722;6, 18:1 n&#8722;9, and 16:0. Spectrophotometry detected no pigments in either the lipovitellins or the cytosol.The egg caloric content was 92 cal\/g, at proportions of 59.8% protein, 20.1% carbohydrate, and 19.9% lipid.SmLV1 and SmLV2 provided 19.5% and 17.1% of the calories, respectively. Both lipovitellins contribute mainlywith proteins (15.8\u201318%), with the input of carbohydrates and lipids being lower than 1.3%.","palabras_clave":"LIPOVITELLINS|ARACHNIDS|LIPIDS|FATTY ACID"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62855,"idioma_id":4,"titulo":"Staring at the cold sun: Blue light regulation is distributed within the Acinetobacter genus.","resumen":"We previously showed that the opportunistic nosocomial pathogen Acinetobacter baumannii is able to sense and respond to light via BlsA, a BLUF (Blue-Light-sensing Using FAD)-domain photoreceptor protein. Here, we extend our previous studies showing that light regulation is not restricted to A. baumannii, but rather widespread within the genus Acinetobacter. First, we found that blue light modulates motility and biofilm formation in many species of the genus, including members of the Acinetobacter calcoaceticus-A. baumannii complex. In many of these species blue light acts as a key factor guiding the decision between motility or sessility at 24uC, whereas in A. baumannii, light inhibits both motility and biofilm formation. We also show that light regulation of motility occurred not only at 24uC but also at 37uC in non-A. baumannii species, contrasting the situation of A. baumannii which only shows photoregulation at 24uC. Second, we show that Acinetobacter baylyi (strain ADP1) BLUF-photoreceptors can functionally replace in vivo the A. baumannii 17978 BlsA protein and that the pathways leading to biofilm formation are inversely regulated at 24uC between these two microorganisms. Finally, we found the presence of predicted genes coding BLUF-containing proteins in all Acinetobacter sequenced genomes, even though the copy number is variable among them. Phylogenetic analysis suggests a common origin for all BLUF domains present in members of this genus, and could distinguish well-differentiated clusters that group together BLUF homologs from different species, a situation particularly clear for members of the ACB complex. Despite a role played by these BLUF domain-containing proteins in the photoregulation observed in the members of the genus Acinetobacter is a likely scenario given our findings in A. baumannii and A. baylyi, further research will contribute to confirm this possibility.","palabras_clave":"ACINETOBACTER|BLUE LIGHT|MOTILITY|BIOFILM FORMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62856,"idioma_id":4,"titulo":"Enhanced dissolution and systemic availability         of albendazole formulated as solid dispersions","resumen":"Solid dispersions (SDs) containing the anthelmintic compound albendazole (ABZ) and either Pluronic 188 (P 188) or polyethylene glycol 6000 (PEG 6000) as hydrophilic carriers were formulated. Drug?polymers interactions in solid state were investigated using different techniques.  Only a 4% of total ABZ was dissolved at 5 min post-incubation, reaching dissolution rates of 32.8% (PEG 6000) and 69.4% (P 188) in SDs. In this way, P 188 was substantially more efficient as ABZ dissolution promoter in comparison to PEG 6000, especially at the initial stages of the dissolution processes (&lt;30 min).  An increased systemic availability (p &lt; 0.001) was obtained when ABZ was administered as ABZ-P 188 SDs, with a 50% enhancement in systemic exposure (AUC values) compared to treatment with an ABZ suspension. Consistently, the Cmax increased 130% (p &lt; 0.001) following treatment with P 188 based SD ABZ formulation. For the ABZ-PEG 6000  SD formulation, the favorable effect on ABZ systemic availability did not reached statistical significance compared to the control group.  The study reported here showed the utility of pharmacokinetic assays performed on mice as a model for preliminary drug formulation screening studies.","palabras_clave":"Pharmacokinetics|Albendazole|Solid Dispersions|Mice"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62857,"idioma_id":5,"titulo":"Los territorios urbano-regionales como medio de innovación. San Luis, ¿laboratorio ciudadano?","resumen":"El artículo consiste en una reflexión crítica sobre los resultados de tres investigaciones decampo sucesivas (2008-2009, 2009-2010 y 2011-2013) que analizan los alcances e impactosdel programa San Luis Digital (SLD) en la provincia de San Luis, Argentina, promovido por elgobierno provincial, con la participación de la Universidad de La Punta, de empresas de basetecnológica, y de organizaciones no gubernamentales (ONG). La pregunta que guía estetrabajo es la siguiente: ¿Es San Luis un laboratorio ciudadano a gran escala? El estudio es decarácter exploratorio y cuali-cuantitativo. Comprende varias actividades: un estudiodocumental de teorías y de las mejores prácticas a nivel internacional sobre el impacto de lasTIC en los usos y hábitos de las personas; la elaboración de indicadores de evaluacióncualitativos y cuantitativos; un diseño muestral de poblaciones e individuos; el diseño de lasentrevistas; el trabajo de campo (dos viajes a cada localidad estudiada); y el procesamiento yanálisis de la información. Los alcances e impactos se estudiaron sobre toda la provincia, pormedio de entrevistas a responsables, participantes y beneficiarios de SLD. El trabajo concluyeque la provincia de San Luis puede considerarse un laboratorio ciudadano implementadoverticalmente, en el que la participación proactiva de los ciudadanos en los procesos deinnovación está prevista en el corto plazo.","palabras_clave":"Educación|Laboratorio Ciudadano|Politicas Públicas|Sociedad del Conocimiento"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62858,"idioma_id":4,"titulo":"Effects of gabaergic phenols on phospholipid bilayers as evaluated by 1H-NMR","resumen":"The phenols propofol and thymol, and recently  carvacrol, eugenol and chlorothymol, have been shown to act as positive  allosteric modulators on GABAA receptor, which is the main inhibitory receptor of  the central nervous system. GABA receptor is an intrinsic membrane protein  which activity may be affected by surface-active compounds and by physical  changes in the membrane. Recently, we demonstrated that these phenols  interacted with the lipid membrane phase, suggesting their anesthetic activity  could be the combined result of their specific (with receptor proteins) as well  as nonspecific (with surrounding lipid molecules) interaction modulating the  supramolecular organization of the receptor environment. In the current study,  by using 1H-NMR spectroscopy, we have investigated the effects of the insertion  and the possible preferential location of the five phenol derivatives with  GABAergic activity on EPC membranes. The re- sults indicate that all compounds  are able to insert in EPC phospholipid vesicles and to locate in the region  between the polar group (choline molecule), the glycerol and the first atoms of  the acyl chains, being the more lipophilic compounds (propofol and  chlorothymol) that seem to prefer a deeper bilayer insertion. The location of  the phenol molecules would reduce the repulsive forces among phospholipids head  groups allowing closer molecular packing and finally diminish- ing the mobility  of the hydrocarbon chains, as revealed by 1H spin relaxation times.","palabras_clave":"Vesiculas unilamelares grandes|phospholipids|GABAergic phenols|1H-NMR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62859,"idioma_id":4,"titulo":"Brain Organization into Resting State Networks Emerges at Criticality on a Model of the Human Connectome","resumen":"&nbsp; The relation between large-scale brain structure and function is an outstanding open problem inneuroscience. We approach this problem by studying the dynamical regime under which realisticspatiotemporal patterns of brain activity emerge from the empirically derived network of human brainneuroanatomical connections. The results show that critical dynamics unfolding on the structuralconnectivity of the human brain allow the recovery of many key experimental findings obtained fromfunctional magnetic resonance imaging, such as divergence of the correlation length, the anomalousscaling of correlation fluctuations, and the emergence of large-scale resting state networks.","palabras_clave":"critical phenomena|brain|connectome|models"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62860,"idioma_id":4,"titulo":"Desiccation resistance along an aridity gradient in the cactophilic fly Drosophila buzzatii: sex-specific responses to stress","resumen":"Stress resistance characters are valuable tools for the study of acclimation potential, adaptive strategies and biogeographic patterns in species exposed to environmental variability. Water stress is a challenge to terrestrial arthropods because of their small size and relatively high area: volume ratio. Fruit flies have been investigated to record adaptive morphological and physiological traits, as well as to test their responses to stressful factors. In this study, we investigate the ability to cope with water stress, by examining variation in desiccation resistance in a species that lives mainly in desert lands. Specifically, we explored the genetic and ecological basis of desiccation resistance in populations of Drosophila buzzatii from Northern Argentina. We used a common garden experiment with desiccation treatments on a number of isofemale lines from four populations along an aridity gradient. Our results revealed significant among-population differentiation and substantial amounts of genetic variation for desiccation resistance. We also detected significant genotype-by-environment and genotype-by-sex interactions indicative that desiccation resistance responses of the lines assayed were environment- and sex-specific. In addition, we observed clinal variation in female desiccation resistance along gradients of altitude, temperature and humidity; that desiccation resistance is a sexually dimorphic trait, and that sexual dimorphism increased along the aridity and altitudinal gradients. Based on current evidence, we propose that the observed sex-specific responses may reflect different life history traits, and survival and reproductive strategies in different ecological scenarios.","palabras_clave":"Intraspecific variation \u0002 |Desiccation resistance \u0002| Clines \u0002 |Sexual dimorphism \u0002"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62861,"idioma_id":4,"titulo":"Small perturbations in a finger-tapping task reveal inherent nonlinearities of the underlying error correction mechanism","resumen":"Time estimation is critical for survival and control of a variety of behaviors, both in humans and other  animals. Time processing in the few hundred milliseconds range, known as millisecond timing, is involved  in motor control, speech generation and recognition, and sensorimotor synchronization like playing music  or finger tapping to an external beat. In finger tapping, a mechanistic explanation in terms of neuronal  activations of how the brain achieves average synchronization against inherent noise and perturbations in  the stimulus sequence is still missing despite considerable research. In this work we show that nonlinear  effects are important for the recovery of synchronization following a perturbation (a step change in stimulus  period), even for perturbation magnitudes smaller than 10% of the period, which is well below the amount  of perturbation needed to display other nonlinear effects like saturation. We build a mathematical model  for the error correction mechanism and test its predictions, and further propose a framework that allows us  to unify the description of the three common types of perturbations and all perturbation magnitudes with  a single set of parameter values. While previous works have proposed that multiple mechanisms\/strategies  are used for correcting different perturbation conditions (based on fitting the model?s parameters separately  to different perturbation types and sizes), our results suggest that the synchronization behavior can be  interpreted as the outcome of a single mechanism\/strategy, and call for a revision of the idea of multiple  strategies.","palabras_clave":"SYNCHRONIZATION|TAPPING |MODELING|ERROR CORRECTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62862,"idioma_id":4,"titulo":"Individual and mixture toxicity of commercial formulations containing glyphosate, metsulfuron-methyl, bispyribac-sodium, and picloram on Rhinella arenarum tadpoles","resumen":"We investigated the effects of four commercialformulations of herbicides (glyphosate [GLY],metsulfuron-methyl [MET], bispyribac-sodium [BIS],and picloram [PIC]) individually, and in three 50:50mixtures (GLY\u2013MET, GLY\u2013BIS, GLY\u2013PIC) on thecommon toad Rhinella arenarum (Anura: Bufonidae)tadpoles. Enzymatic parameters such as, glutathione\u2013MET, GLY\u2013BIS, GLY\u2013PIC) on thecommon toad Rhinella arenarum (Anura: Bufonidae)tadpoles. Enzymatic parameters such as, glutathioneRhinella arenarum (Anura: Bufonidae)tadpoles. Enzymatic parameters such as, glutathioneS-transferase (GST), butyrylcholinesterase (BChE)and acetylcholinesterase (AChE) activities, as well aserythrocyte nuclear abnormalities (ENA) were studied.Interactions between herbicides in mixtures wereevaluated and classified as additive, synergistic, orantagonistic. Toxicity results (48-h LC50) showed thatPIC was the most toxic herbicide, followed by BIS,GLY, and MET, while GLY\u2013PIC was the most toxicmixture, followed by GLY\u2013BIS, and GLY\u2013MET. Allcommercial herbicide formulations and their mixturessignificantly inhibited BChE activity in exposed tadpoles.The AChE activity was also inhibited by allherbicides and their mixtures, except by GLY\u2013-transferase (GST), butyrylcholinesterase (BChE)and acetylcholinesterase (AChE) activities, as well aserythrocyte nuclear abnormalities (ENA) were studied.Interactions between herbicides in mixtures wereevaluated and classified as additive, synergistic, orantagonistic. Toxicity results (48-h LC50) showed thatPIC was the most toxic herbicide, followed by BIS,GLY, and MET, while GLY\u2013PIC was the most toxicmixture, followed by GLY\u2013BIS, and GLY\u2013MET. Allcommercial herbicide formulations and their mixturessignificantly inhibited BChE activity in exposed tadpoles.The AChE activity was also inhibited by allherbicides and their mixtures, except by GLY\u201350) showed thatPIC was the most toxic herbicide, followed by BIS,GLY, and MET, while GLY\u2013PIC was the most toxicmixture, followed by GLY\u2013BIS, and GLY\u2013MET. Allcommercial herbicide formulations and their mixturessignificantly inhibited BChE activity in exposed tadpoles.The AChE activity was also inhibited by allherbicides and their mixtures, except by GLY\u2013\u2013PIC was the most toxicmixture, followed by GLY\u2013BIS, and GLY\u2013MET. Allcommercial herbicide formulations and their mixturessignificantly inhibited BChE activity in exposed tadpoles.The AChE activity was also inhibited by allherbicides and their mixtures, except by GLY\u2013\u2013BIS, and GLY\u2013MET. Allcommercial herbicide formulations and their mixturessignificantly inhibited BChE activity in exposed tadpoles.The AChE activity was also inhibited by allherbicides and their mixtures, except by GLY\u2013\u2013BIS. The inhibition of GST activity was onlysignificant for GLY, MET, PIC, and GLY\u2013MET.A significant increase in the frequency of ENAwas found for tadpoles exposed either to commercialherbicide formulations or to mixtures, exceptfor GLY. All the mixtures showed synergism forBChE activity while for AChE only the GLY\u2013\u2013MET.A significant increase in the frequency of ENAwas found for tadpoles exposed either to commercialherbicide formulations or to mixtures, exceptfor GLY. All the mixtures showed synergism forBChE activity while for AChE only the GLY\u2013\u2013MET and GLY\u2013PIC mixtures acted synergistically.GLY\u2013MET showed synergism for GST, whereasfor ENA, the mixture GLY\u2013BIS was antagonistic.This study with R. arenarum tadpoles demonstratesthat the interactions between three of the mostintensively used herbicides in soybean cropsresults in synergistic effects on mortality and neurotoxicityand synergistic or additive effects ingenotoxicity.\u2013PIC mixtures acted synergistically.GLY\u2013MET showed synergism for GST, whereasfor ENA, the mixture GLY\u2013BIS was antagonistic.This study with R. arenarum tadpoles demonstratesthat the interactions between three of the mostintensively used herbicides in soybean cropsresults in synergistic effects on mortality and neurotoxicityand synergistic or additive effects ingenotoxicity.\u2013MET showed synergism for GST, whereasfor ENA, the mixture GLY\u2013BIS was antagonistic.This study with R. arenarum tadpoles demonstratesthat the interactions between three of the mostintensively used herbicides in soybean cropsresults in synergistic effects on mortality and neurotoxicityand synergistic or additive effects ingenotoxicity.\u2013BIS was antagonistic.This study with R. arenarum tadpoles demonstratesthat the interactions between three of the mostintensively used herbicides in soybean cropsresults in synergistic effects on mortality and neurotoxicityand synergistic or additive effects ingenotoxicity.R. arenarum tadpoles demonstratesthat the interactions between three of the mostintensively used herbicides in soybean cropsresults in synergistic effects on mortality and neurotoxicityand synergistic or additive effects ingenotoxicity.","palabras_clave":"mixture toxicity | Rhinella arenarum"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62863,"idioma_id":4,"titulo":"High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE","resumen":"This article reports a simple methodology using dispersive liquid?liquid microextraction  (DLLME) combined with CZE. It has been applied for the simultaneous determination of  phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids  and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO).  The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGEwas optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration  a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004?0.251 mg\/kg), recoveries (89.4?101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples","palabras_clave":"CZE|Dispersive liquid-liquid microextraction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62864,"idioma_id":4,"titulo":"Olive oil by capillary electrophoresis: characterization and genuineness","resumen":"Olive oil, obtained from Olea europaea L. (Oleaceae) fruits, is an important ingredient in the Mediterranean diet.  The purpose of this paper is to review and evaluate olive oil analysis using capillary electrophoresis (CE). This review covers a  selection of the literature published on this topic over the past decade. The current state of the art of the topic is evaluated, with  special emphasis on separation conditions, analysis purpose, and analytes investigated. CE has been used to characterize or to  carry out authenticity studies. Particular attention has been focused on the botanical origin because high-quality monovarietal  olive oils have been recently introduced on the markets and their quality control requires the development of new and powerful  analytical tools as well as new regulations to avoid fraud. CE represents a good compromise between sample throughput, sample  volume, satisfactory characterization, and sustainability for the analysis of target compounds present in olive oils.","palabras_clave":"olive oil|capillary electrophoresis|characterization|adulterant|classification"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62865,"idioma_id":4,"titulo":"Refinement of neuronal synchronization with gamma oscillations in the medial prefrontal cortex after adolescence","resumen":"The marked anatomical and functional changes taking place in the medial prefrontalcortex (PFC) during adolescence set grounds for the high incidence ofneuropsychiatric disorders with adolescent onset. Although circuit refinement throughsynapse pruning may constitute the anatomical basis for the cognitive differencesreported between adolescents and adults, a physiological correlate of circuitrefinement at the level of neuronal ensembles has not been demonstrated. We haverecorded neuronal activity together with local field potentials in the medial PFC ofjuvenile and adult mice under anesthesia, which allowed studying local functionalconnectivity without behavioral or sensorial interference. Entrainment of pyramidalneurons and interneurons to gamma oscillations, but not to theta or beta oscillations,was reduced after adolescence. Interneurons were synchronized to gamma oscillationsacross a wider area of the PFC than pyramidal neurons, and the span of interneuronsynchronization was shorter in adults than juvenile mice. Thus, transition fromchildhood to adulthood is characterized by reduction of the strength and span ofneuronal synchronization specific to gamma oscillations in the mPFC. The morerestricted and weak ongoing synchronization in adults may allow a more dynamicrearrangement of neuronal ensembles during behavior and promote parallelprocessing of information.","palabras_clave":"adolescencia|prefrontal|oscilaciones|interneuronas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62866,"idioma_id":4,"titulo":"The effect of previous exposure to nicotine on nicotine place preference","resumen":"Rationale Prior exposure to drugs of abuse may increase ordecrease the reinforcing effects of the drug in later consumptions.Based on the initial locomotor activity (LA)response to an acute drug administration or to novelty inan open-field arena, animals can be classified as low or highLA responders (LR or HR). Few studies have used thisclassification with nicotine, and the results are controversial.Some authors suggested that nicotine can induceconditioned-place preference (CPP) following prior nicotineexposure, whereas others suggested that previous nicotineexposure extinguishes nicotine-CPP.Objective To explore if the administration of nicotine in anovel environment without explicit behavioral consequencesto classify animals in low and high nicotine responders(LNR and HNR) could affect the establishment of nicotineCPP in male Sprague?Dawley rats.Results Prior exposure to a single dose of nicotine (0.4 mg\/kg, subcutaneously) induced CPP in LNR rats after 14 daysof conditioning (seven-trial) but not after two or eight conditioningdays. In contrast, HNR rats did not show CPPunder any condition. In addition, our results indicated thatprevious exposure to nicotine decreased its rewardingeffects in eight conditioning days CPP (four-trial), whichcan be regularly established without prior exposure tonicotine.Conclusion The results suggested that response to a singleexposure to nicotine predicts the acquisition of nicotinepreference in a 14-day conditioning protocol only for LNRrats. Thus, our findings demonstrated the relevance of usingLNR and HNR classification when the individual susceptibilityto nicotine preference is studied.","palabras_clave":"NICOTINA|CONDICIONAMIENTO|ADICCION|VULNERABILIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62867,"idioma_id":5,"titulo":"Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models","resumen":"Mycobacterium tuberculosis has a considerable degree of genetic variability resulting in different epidemiology and disease outcomes. We evaluated the pathogen-host cell interaction of two genetically closely-related multidrug-resistant M. tuberculosis strains of the Haarlem family, namely the strain M, responsible for an extensive multidrug-resistant tuberculosis outbreak, and its kin strain 410 which caused a single case in two decades. Intracellular growth and cytokine responses were evaluated in human monocyte-derived macrophages and dU937 macrophage-like cells. In monocyte-derived macrophages, strain M grew more slowly and induced lower levels of TNF-alfa and IL-10 than 410, contrasting with previous studies with other strains, where a direct correlation was observed between increased intracellular growth and epidemiological success. On the other hand, in dU937 cells, no difference in growth was observed between both strains, and strain M induced significantly higher TNF-alfa levels than strain 410. We found that both cell models differed critically in the expression of receptors for M. tuberculosis entry, which might explain the different infection outcomes. Our results in monocyte-derived macrophages suggest that strain M relies on a modest replication rate and cytokine induction, keeping a state of quiescence and remaining rather unnoticed by the host. Collectively, our results underscore the impact of M. tuberculosis intra-species variations on the outcome of host cell infection and show that results can differ depending on the in vitro infection model","palabras_clave":"Multidrug resistance Mycobacterium tuberculosis|Monocyte derived macrophages|U937|Virulence|Cytokines"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62868,"idioma_id":4,"titulo":"Characterizing Fault-Tolerant Systems by Means of Simulation Relations","resumen":"In this paper, we study a formal characterization of fault-tolerant behaviors of systems via simulation relations. This formalization makes use of particular notions of simulation and bisimulation in order to compare the executions of a system that exhibit faults with executions where no faults occur. By employing variations of standard (bi)simulation algorithms, our characterization enables us to algorithmically check fault-tolerance, i.e., to verify that a system behaves in an acceptable way even under the occurrence of faults.  Our approach has the benefit of being simple and supporting an efficient automated treatment. We demonstrate the practical application of our formalization through some well-known case studies, which illustrate that the main ideas behind most fault-tolerance mechanisms are naturally captured in our setting.","palabras_clave":"FAULT-TOLERANCE|BISIMULATION|TEMPORAL LOGICS|DEONTIC LOGICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62869,"idioma_id":4,"titulo":"Synthesis, characterization and magnetic behavior of Mg-Fe-Al mixed oxides based on layered double hydroxide","resumen":"In the present work, Mg?Al?Fe layered double hydroxides were prepared by coprecipitation reaction  with hydrothermal treatment. The characterization of precursors and their corresponding calcinated  products (mixedoxides) were carried out by Xray diffraction, X-ray photoelectron spectroscopy (XPS),  Termogravimetric Analysis and Differential Scanning Calorimetry, Diffuse Reflectance UV?vis spectroscopy, specific surface area, Mössbauaer and magnetic properties. The Fe3+ species were observed in tetrahedrally and octahedral coordination in brucite layered. The XPS analysis shows that the Fe3+ ions can be found in two coordination environments (tetrahedral and octahedral) as mixed oxides, and as spinel-structure. Oxides show a decrease in the specific surface areas when their on loading is increased. The magneticand Mössbauaer response show that MgAlFe mixed oxides are different behaviours such as different population ratios of ferromagnetic, weak-ferromagnetic, paramagnetic and superparamagnetic phases. The better crystallization of spinel structure with increased temperature, is correlated with the improved magnetic properties","palabras_clave":"Layered doubled hydroxides |Coprecipitation |Mixed oxides|Magnetic properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62870,"idioma_id":4,"titulo":"Chemical composition, antioxidant activity and anti-lipase activity of Origanum vulgare and Lippia turbinata essential oils","resumen":"Many essential oils have shown antioxidant activity. Oregano and Lippia turbinata essential oils could be an alternative as antioxidant agents. This study reported the chemical composition, phenolic content, antioxidant and anti-lipase activity of oregano and Lippia essential oils. The major compounds found in Oregano essential oil were gama-terpinene (32.10%), alfa-terpinene (15.10%), para-cymene (8.00%) and thymol (8.00%). In Lippia essential oil, alfa-limonene (76.80%) and 1,8-cineole (4.95%) represented the major compounds. Oregano essential oil had higher phenolic content (12.47 mg galic acid\/mL) and DPPH scavenging activity (IC50 0.357 g\/mL) than Lippia essential oil (7.94 mg galic acid\/mL and IC50 0.400 g\/mL, respectively). Both essential oils had similar antioxidant indexes (about 1.2) determined by Rancimat. Moreover, Oregano essential oil had also higher anti-lipase activity (IC50 5.09 and 7.26 g\/mL). Higher phenolic content in the essential oils was related with higher scavenging and anti-lipase activities. Oregano and Lippia essential oils could be used as natural antioxidants on food products.","palabras_clave":"ESSENCIAL|LIPASE|ANTIOXIDANT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62871,"idioma_id":4,"titulo":"Effect of peanut skin extract on chemical and sensory stability of salami","resumen":"BACKGROUND: Peanut skin extracts have proved antioxidant properties in different food products. The objective of this study was to evaluate the effect of peanut skin extract as natural preserving compounds on chemical stability and sensory properties of salami during storage.   RESULTS: Peanut skin extract (PSE) was obtained with ethanol-water and added during the preparation of salami samples. Raw salami samples were cured and stored at 15 C and 65% RH. Moisture, peroxide value, conjugated dienes, free fatty acids and sensory descriptive attributes were evaluated on the samples. Peroxide values increased during storage in all samples and were 82.9 in Control (salami without additives), 18.0 in salami with 0.2 g kg-1 PSE (E0.02), 13.0 in salami with 1.0 g kg-1 PSE (E0.1), and 0.63 meqO2 kg-1 in salami with BHT after 42 days of storage. BHT and E0.1 treatments had lower increase in the intensity of oxidized flavor and lower decrease in the intensity of salami flavor.   CONCLUSION: Chemical indicators and descriptive results indicated that the peanut skin extract retards lipid oxidation and preserve sensory properties of salami prolonging its shelf-life.","palabras_clave":"PEANUT|SALAMI|ANTIOXIDANT|PHENOL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62872,"idioma_id":4,"titulo":"Activity of natural compounds from peanut skins on Fusarium verticillioides growth and fumonisin B1 production","resumen":"The objectives of this investigation were to evaluate the antifungal and antimycotoxigenic properties of peanut skin extracts (PSE) against Fusarium &nbsp;verticillioides. The PSE were prepared by a multisolvent extraction procedure, and the activity on growth parameters and fumonisin B1 (FB1) production of the three PSE was explored at different concentrations on potato dextrose agar and in artificially infected maize kernels, respectively. The results demonstrated that all PSE had a significant influence on growth rate or lag phase of F. verticillioides. The yellow and purple (250?500 g\/mL) extracts decreased growth rate, whereas brown extract extended the lag phase. Only the yellow extract at 62.5 g\/mL was able to affect both growth rate and lag phase. With respect to mycotoxin production a significant stimulation on FB1 production was observed with purple (62.5 g\/mL) and brown (250 g\/mL) extracts. In contrast, a significant decreased in FB1 was observed at 62.5 g\/mL of yellow extract. These findings showed that natural compounds from PSE possess inhibitory effects on F. verticillioides growth and mycotoxin production. Thus, PSE could be use as an alternative to minimize fungal contamination.                                   ","palabras_clave":"PEANUT SKIN|ANTIFUNGAL ACTIVITY|FUSARIUM VERTICILLIOIDES|FUMONISIN B1|BY-PRODUCTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62873,"idioma_id":4,"titulo":"Efficacy of novel benznidazole solutions during the experimental infection with 3 Trypanosoma cruzi","resumen":"Chagas´ disease is caused by the protozoan parasite Trypanosoma cruzi. About 8 million people throughout Latin America are infected causing approximately 10,000 deaths annually. Benznidazole, available as unique 100 mg tablets in many of the endemic countries, is currently the drug of choice for the specific treatment of this condition. Despite of the large number of pediatric patients infected, there are no commercial liquid dosage forms available to treat this trypanosomiasis. This work showed that novel benznidazole-water-polyethylene glycol 400 solutions are active against T. cruzi in a murine model of Chagas´ disease. Present results constitute the first demonstration on the usefulness of benznidazole solutions in infected mice","palabras_clave":"Trypanosoma cruzi|benznidazol"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62874,"idioma_id":4,"titulo":"Trophic relationships between macroinvertebrates and fish in a pampean lowland stream (Argentina)","resumen":"ABSTRACT. The diet and trophic relationships between the macroinvertebrates Phyllogomphoides joaquini Rodrigues Capítulo, 1992 andCoenagrionidae (Odonata), Chironomidae (Diptera), Diplodon delodontus (Lamarck, 1919) (Bivalvia: Hyriidae), and Pomacea canaliculata(Lamarck, 1822) (Gastropoda: Ampulariidae) and the fishes Pimelodella laticeps Eigenmann, 1917 (Heptapteridae) and Bryconamericus iheringii(Boulenger, 1887) (Characidae) in a temperate lowland lotic system in Argentina were assessed on the basis of gut contents and stable-isotopeanalyses. The feeding strategies were analyzed by the Amundsen method. Relative food items contribution for the taxa studied indicated ageneralist-type trophic strategy. In macroinvertebrates, in general, the values of stable isotope confirmed the result of the analysis of gut contents.With the fish, stable-isotope analysis demonstrated that both species are predators, although B. iheringii exhibited a more omnivorous behaviour.These feeding studies allowed us to determine the trophic relationships among taxa studied. Detritus and diatoms were a principal source of foodfor all the macroinvertebrates studied. In La Choza stream the particulate organic matter is a major no limited food resource, has a significantinfluence upon the community.","palabras_clave":"MACROINVERTEBRATES|FISH|TROPHIC RELATIONSHIPS|TEMPERATE PLAIN STREAMS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62875,"idioma_id":4,"titulo":"Whey protein concentrate gels with different sucrose content: instrumental texture measurements and sensory perception","resumen":"Abstract  Correlations between instrumental texture, sensory texture and sweetness perception were studied in whey protein concentrate (WPC) gels at different pH (4 and 7), sucrose (0?40%, w\/w) and whey protein (10?20% w\/w) content. The presence of sucrose modified the structure of WPC gels, mainly at pH 4, making the gel structure more homogeneous and with smaller pores. Sucrose also increased the solid behaviour of gels, their water holding capacity, hardness and adhesiveness. Sweetness perception decreased as protein concentration increased, and was higher in gels at pH 4 than in gels at pH 7. A good correlation was obtained between the instrumental and sensory attributes hardness, cohesiveness and elasticity.","palabras_clave":"whey protein|texture |sensory profile"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62876,"idioma_id":4,"titulo":"Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive","resumen":"Background: Microcin J25 (MccJ25) is a plasmid-encoded antibiotic peptide produced by Escherichia coli (E. coli). MccJ25 enters into the sensitive E. coli strains by the outer membrane receptor FhuA and the inner membrane proteins TonB, ExbB, ExbD and SbmA. The resistance of Salmonella enterica serovar Typhimurium (S. Typhimurium) to MccJ25 is attributed to the inability of its FhuA protein to incorporate the antibiotic into the cell.   Results: In this work we demonstrate that S. Typhimurium becomes notably susceptible to MccJ25 when replicating within macrophages. In order to determine the possible cause of this phenomenon, we studied the sensitivity of S. Typhimurium to MccJ25 at conditions resembling those of the internal macrophage environment, such as low pH, low magnesium and iron deprivation. We observed that the strain was only sensitive to the antibiotic at low pH, leading us to attribute the bacterial sensitization to this condition. A MccJ25-resistant E. coli strain in which fhuA is deleted was also inhibited by the antibiotic at low pH. Then, we could assume that the MccJ25 sensitivity change observed in both E. coli fhuA and S. Typhimurium is mediated by a MccJ25 uptake independent of the FhuA receptor. Moreover, low pH incubation also sensitized S. Typhimurium to the hydrophobic antibiotic novobiocin, which does not affect enteric bacteria viability because it is unable to penetrate the bacterial outer membrane. This observation supports our hypothesis about low pH producing a modification in the bacterial  membrane permeability that allows an unspecific MccJ25 uptake. On the other hand, MccJ25 inhibited S. Typhimurium when cells were preincubated in acidic pH medium and then treated at neutral pH with the antibiotic.  Conclusions: Our results suggest that acidic condition does not alter MccJ25 hydrophobicity but irreversibly modifies bacterial membrane permeability. This would allow an unspecific antibiotic uptake into the cell. From our data it is possible to infer that intracellular pathogenic strains, which are in vitro resistant to MccJ25, could become susceptible ones in vivo. Therefore, the MccJ25 action spectrum would be broader than what in vitro experiments indicate.","palabras_clave":"MICROCIN|SALMONELLA|TARGET|CELL LINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62877,"idioma_id":4,"titulo":"On twisted conjugacy classes of type D in sporadic simple groups","resumen":"We determine twisted conjugacy classes of type D associated withthe sporadic simple groups. This is an important step in the program of the classification of finite-dimensional pointed Hopf algebras with non-abelian coradical. As a by-product we prove that every complex finite-dimensional pointed Hopf algebra over the group of automorphisms of M12, J2, Suz, He, HN, T is the group algebra. In the appendix we improve the study of conjugacy classes of type D of sporadic simple groups.&nbsp;","palabras_clave":"Hopf algebras|Nichols algebras|Racks"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62878,"idioma_id":4,"titulo":"Structural and functional properties of venous wall: Relationship between elastin, collagen and smooth muscle components and viscoelastic properties","resumen":"The purpose of this work was (1) to analyze the viscoelastic behavior of different venous segments and their differences, considering the structural characteristics (elastin, collagen and smooth muscle content) of the venous wall; and (2) to analyze the venous biomechanical behavior by means of the histological characteristics of the veins. In this study, nine healthy male Corriedale sheep, weighing 25-35 kg and aged between 12-16 months, were included. One vein was selected from each animal to evaluate its biomechanical properties: (a) anterior vena cava, (b) right jugular vein, and (c) right femoral vein. Each selected vein was instrumented with pressure and diameter sensors. After excision, a small ring-shaped sample was set apart from each segment for histological analysis. The amounts of elastin, collagen and smooth muscle were correlated to calculated biomechanical parameters (high and low pressure compliance and viscosity). Results: High-pressure compliance values were always lower than low-pressure compliance values (p&lt;0.05), in jugular, cava and femoral veins. Both low and high pressure compliance values calculated for the vena cava segments were the highest of those corresponding to jugular and femoral vein segments (p&lt;0.05). On the other hand, the low and high-pressure compliance values calculated for femoral vein segments were the lowest (p&lt;0.05). There is a negative association between the absolute amounts of smooth muscle and collagen tissue in the venous wall (p&lt;0.05). The association between venous wall viscosity and the relative amounts of vascular smooth muscle was positive (p&lt;0.05). The collagen relative content of the vein walls was negatively associated with both low and high-pressure compliance (p&lt;0.05). Conclusions: (1) the viscoelastic behavior of the venous wall varies depending on the vascular territory, and it is associated with the variation of the histological structure. These differences involve muscle (both smooth and striated), elastin and collagen contents. (2) In addition, the quantity of collagen was negatively correlated with high and low-pressure compliances and (3) the smooth muscle content was higher in peripheral veins and is positively correlated with venous wall viscosity. ","palabras_clave":"viscoelastic properties|venous wall|structural and fucntional properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62879,"idioma_id":4,"titulo":"Emulsifying and foaming properties of beta-lactoglobulin modified by heat treatment","resumen":"          The effects of heat treatment on emulsifying  properties of b-lactoglobulin  were studied in order to compare them with previous studies on foaming properties.  Both of them are closely linked to the structural changes on the protein. Aliquots from 5.5% (w\/v) b-lactoglobulin solution in 20 mM phosphate buffer pH 6.8  were heated at 85 ºC  for different time periods, from 1 to 15 min. Protein solubilities were  measured for unheated and heated b-lactoglobulin  samples. Protein-stabilized O\/W emulsions were prepared with  these samples and corn oil. Droplet size distribution in the emulsions and emulsifying  activity index were determined for each system, as parameters of the  emulsifying ability of the protein. Emulsion  stability was estimated from three different methods: backscattering, determination of the remaining protein concentration  after creaming and monitoring the oiling off process. With the assayed methodology, heat treatment of b-lactoglobulin led to different  effects on foaming and emulsifying properties of the protein, depending on the  time of heating. For shorter times of heating, both foamability and foam stability  improved, while emulsifying properties diminished. After 10 min of heating at 85 °C, both foaming and  emulsifying properties diminished. Formation time scales, as well as size of the sedimentable aggregates  and their steric effect on the interfacial film, play an important role in  explaining these differences between foaming and emulsifying properties.          &nbsp;  ","palabras_clave":"BETA-LACTOGLOBULIN|HEATING TREATMENT|PROTEIN AGGREGATES|FOAMING PROPERTIES|EMULSIFYING PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62880,"idioma_id":4,"titulo":"First dendroarchaeological dates of prehistoric contexts in South America: chullpas in the Central Andes","resumen":"Despite the existence of long-lived tree species with excellent tree-ring characteristics, the use of dendrochronological techniques for dating archaeological contexts has been very limited in South America. Recent research in the Bolivian Altiplano of the Central Andes has yielded a network of Polylepis tarapacana chronologies that goes back to the 13th century. This species was regularly used by local populations since pre-Columbian times as raw material for beams, lintels, hooks and other architectural features in houses, storage chambers, and sepulchers. The aim of this study is to argue in favor of the potential of P. tarapacana for providing high resolution dates of significant archaeological events during the pre-Columbian era. The chronology of chullpas (burial towers and storage chambers) in the southern Andean Altiplano is used as a case study. Growth rings from P. tarapacana have provided several centennial-length dendrochronological records including a reference regional chronology covering the last 786 years in the Central Andean highlands. Based on this long reference chronology calendar years were assigned to woody pieces from pre-Hispanic chullpas. Dendroarchaeological results are consistent with contextual evidence and most radiocarbon dates from woody samples and related archaeological items. Our results indicate that P. tarapacana has a high potential for providing annually-resolved tree ringdates for archaeological contexts in the Altiplano since the early 13th century.","palabras_clave":"DENDROARCHAEOLOGY|POLYLEPIS TARAPACANA|ANDEAN ARCHAEOLOGY|ANCESTOR CULTS|CHULLPAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62881,"idioma_id":4,"titulo":"Continental-scale temperature variability during the past two millennia","resumen":"Past global climate changes had strong regional expression. To elucidate their spatio-temporal pattern, we reconstructed past temperatures for seven continental-scale regions during the past one to two millennia. The most coherent feature in nearly all of the regional temperature reconstructions is a long-term cooling trend, which ended late in the nineteenth century. At multi-decadal to centennial scales, temperature variability shows distinctly different regional patterns, with more similarity within each hemisphere than between them. There were no globally synchronous multi-decadal warm or cold intervals that define a worldwide Medieval Warm Period or Little Ice Age, but all reconstructions show generally cold conditions between AD 1580 and 1880, punctuated in some regions by warm decades during the eighteenth century. The transition to these colder conditions occurred earlier in the Arctic, Europe and Asia than in North America or the Southern Hemisphere regions. Recent warming reversed the long-term cooling; during the period AD 1971?2000, the area-weighted average reconstructed temperature was higher than any other time in nearly 1,400 years.","palabras_clave":"PALAEOCLIMATE AND PALAEOCENOGRAPHY|CLIMATE SCIENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62882,"idioma_id":4,"titulo":"Silvopastoral use of Nothofagus antarctica in Southern Patagonian forests, influence over net nitrogen soil mineralization","resumen":"In most temperate forest, nitrogen (N) is considered a limiting factor. This would be more important in extreme environments, as Nothofagus antarctica forests, where the antecedents are scarce. Thinning practices in N. antarctica forests for silvopastoral uses may modify the soil N dynamics. Therefore, the objective of this work was to evaluate the temporal variation of soil N in these systems. The mineral extractable soil N, net nitrification and net N mineralization were evaluated under different crown cover and two site quality stands. The mineral N extractable (NH4+-N + NO3--N) was measured periodically. Similarly, net nitrification and net N mineralization were estimated through the technique of incubation of intact samples with tubes. The total mineral extractable N concentration varied between crown cover and dates, with no differences among site class. The lowest and highest values were found in the minimal and intermediate crown cover, respectively. In the higher site quality stand, the annual net N mineralization was significant lower in the minimal crown cover reaching 11 kg N ha-1 yr-1, whilst the average in the others crown cover was 63 kg N ha-1 yr-1. In the lower site quality stand there was no differences among crown cover. The same pattern was found for net nitrification. Thinning practices for silvopastoral use of these forests, keeping intermediate crown cover values, would affect neither N mineralization nor nitrification. Nevertheless, the results suggest that high removal of trees may decrease N mineralization and nitrification in higher quality sites.","palabras_clave":"Nitrogen dynamic |forest soil|silvopastoral system|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62883,"idioma_id":5,"titulo":"Regeneración por semillas en bosques nativos de Nothofagus antarctica bajo uso silvopastoril en Patagonia Sur, Argentina","resumen":"Se discute la producción de semillas y regeneración del Nothofagus antarctica (ñire).","palabras_clave":"Regeneración|Semillas|Ñire|Silvopastoril"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62884,"idioma_id":4,"titulo":"Genetic diversity in a world germplasm collection of tall fescue","resumen":"Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions ofFestucaspecies from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of polymorphic information content ranged from 0.627 to 0.840. In addition, the accessions analyzed by flow cytometry showed different ploidy levels  (diploid, tetraploid, hexaploid and octaploid), placing in evidence that the world germplasm collection consisted of multiple species, as previously suggested. Interestingly, almost all accessions of INTA germplasm collection were true hexaploid tall fescue, belonging to two eco-geographic races (Continental and Mediterranean). Finally, the data  presented revealed an ample genetic diversity of tall fescue showing the importance of preserving the INTA collection for future breeding programs.","palabras_clave":"SSR MARKERS|GENETIC DIVERSITY|FLOW CYTOMETRY|TALL FESCUE|GENETIC RESOURCES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62885,"idioma_id":4,"titulo":"Optimization of environmentally benign polymers based on thymine and poly vinyl sulfonate using Plackett-Burman design and surface response","resumen":"Traditional approaches to the development of integrated circuits involve the use and\/or manufacture of toxic materials that have a potential environmental impact. An extensive research has been done to design environmentally benign synthetic polymers containing nucleic acid bases, which can be used to enhance the photoresistor technologies. Water soluble, environmentally benign photopolymers of 1-(4-vinylbenzyl) thymine (VBT) and vinylphenylsufonate (VPS) undergo a photodimerization reaction when exposed to low levels of ultraviolet irradiation leading to an immobilization of the copolymer on a variety of substrates. Plackett\u2013Burman design (PBD) and Central Composite Design (CCD) were applied to identify the signi&#64257;cant factors in&#64258;uencing the polymer crosslinking and dye adsortion processes, which are relevant in the fabrication of copolymer films for potential photoresist use. The PBD results assigned a maximum absorption signal of 0.67, while optimal conditions obtained in this experiment following the CCD method predictions provided a response of 0.83&#61617;0.03, being a solid foundation for further use of this methodology in the production of potential photoresistors. The pH effect was relevant for low concentrations but not significant for higher concentrations. To the best of our knowledge, this was the &#64257;rst report applying statistical experimental designs to optimize the crosslinking of thymine based polymers.","palabras_clave":"EXPERIMENTAL DESIGN|PHOTOPOLYMERS|PHOTORESISTORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62886,"idioma_id":4,"titulo":"Shielding properties of CuNiZn ferrite in the radio frequency range","resumen":"Shielding properties of copper-doped NiZn ferrites in the frequency range 1 MHz?1.8 GHz, were explored. Samples of composition CuxNi(0.4-x)Zn0.6Fe2O4  with x=0.0; 0.1; 0.2; 0.3; 0.4, were prepared by the ceramic method. Powders  and pellets were sintered at 1100oC for 2 h. The effect of  compositional variation on structural, dielectric and magnetic properties was  investigated. X-ray diffraction measurements confirmed the formation of  single-phase cubic spinel structure at the selected sintering temperature. The  addition of copper promotes grain growth, increases samples density and  modifies saturation magnetization, permeability and permittivity in the  frequency range from 1MHz to 1.8GHz. The electric resistivity decreases with Cu  substitution. Microwave absorption, in the explored frequency range is strongly  dependent on the amount of Cu in the ferrite. As the RL values for  all the samples are above 35 dB, they can all be considered as shielding  materials. The optimum attenuation occurs for x=0.2, reaching a minimum  reflection loss of almost 60 dB at 10 MHz and presenting an attenuation  bandwidth of at least 3 MHz. ","palabras_clave":"ATTENUATION PROPERTIES|NiZn FERRITES|CuZn FERRITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62887,"idioma_id":4,"titulo":"Cation distribution and magnetic properties of LiMnZn ferrites","resumen":"Spinelcompounds Li0.5x Zn0.6-x Mn0.4 Fe2+0.5x O4(x=0.0; 0.1; 0.2; 0.4) were produced by the self-combustion sol-gel method. The obtained powderswere calcined at 1000ºCfor 2 hours in Ar atmosphere. The structural and microstructuralcharacteristics of the resulting samples were studied by magnetizationmeasurements and X-ray powder diffraction in combination with the Rietveldmethod. Based on magnetization results, a cation distribution model is proposedand is tested with Rietveld refinements. The observed increase in saturationmagnetization with Li content in the structure is explained by an unusualdistribution of Zn2+ ions in octahedral sites.","palabras_clave":"CERAMICS|OXIDE MATERIALS|CRYSTAL STRUCTURE |MAGNETIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62888,"idioma_id":4,"titulo":"Coercivity enhancement of hexagonal ferrites","resumen":"Hexagonalferrites have been widely used as permanent magnets since their discovery inthe 1950s. In spite of their relatively modest magnetic properties, ferritemagnets still show the best performance to cost ratio and big efforts are beingmade in improving their magnetic capabilities by using different synthesismethods and compositions. Differentscientific investigations and techniques (Mössbauer spectrometry, X-raydiffraction, magnetic measurements) have allowed to optimize the permanentmagnet properties of rare earth substituted hexagonal ferrite magnets such asLa-Co and&nbsp; Nd-Co Sr and Ba ferrites.However, the solubility of rare earth ions in M-type hexaferrite is very lowand their introduction leads to the formation of secondary phases, which mustbe avoided in order to obtain permanent magnets with optimal properties. We report resultson enhanced coercivity of hexagonal ferrites with Nd-Co substitution. Thesynthesis of this kind of ferrite is performed with a defficient,non-stoichiometric iron content (ratio Fe\/ Sr1\u2013xRx of 11 instead of12) in order to diminish secondary phases.&nbsp; Comparison with samples of the samecomposition and stoichiometric formulation is made. A further improvement of both intrinsic andmagnetic properties of the Nd-Co substituted ferrite can be obtained byadjusting the chemical composition.","palabras_clave":"HEXAGONAL FERRITES|COERCIVITY ENHANCEMENT|RARE-EARTH SUBSTITUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62889,"idioma_id":5,"titulo":"MODELO DE ANEGAMIENTO Y ESTRATEGIA DE PREDICCIÓN-PREVENCIÓN DEL  RIESGO DE INUNDACIÓN EN ÁREAS DE LLANURA: EL SUDESTE DE LA  PROVINCIA DE BUENOS AIRES COMO CASO DE ESTUDIO","resumen":"En el sector sudeste de la provincia de Buenos Aires se encuentra la llanura interserrana bonaerense, limitada por los sistemas serranos de Tandilla y Ventania, dedicada casi exclusivamente a la producción agrícola. La alta productividad de la zona está ligada fuertemente a la disponibilidad de agua, tanto en forma superficial como subterránea, por lo que las variaciones climáticas tienen un fuerte impacto en la producción y el desarrollo de la región. En este documento se analizan las últimas tres inundaciones ocurridas en la cuenca del río Quequén Grande, correspondientes a los años 1980, 1998 y 2002. Para esto se utilizaron datos de precipitación, caudal e imágenes de satélite, todo enmarcado dentro de un Sistema de Información Geográfica (SIG). Se estableció el comportamiento de la lluvia y los caudales antes y durante los eventos de inundación, tiempos de respuesta y datos estadísticos relevantes. Con las imágenes de satélite y utilizando técnicas clásicas de procesamiento digital se obtuvieron mapas de áreas inundables para los años 1998 y 2002, logrando la cuantificación de las zonas afectadas en cada evento. Se generó además un modelo de zonas con mayores probabilidades de anegamiento utilizando información de suelos, espesor de zona no saturada, pendiente y orientación del terreno. Cada una de estas coberturas fue rasterizada y reclasificada según su potencial aporte a la acumulación de agua en superficie, asignado valores altos a aquellas zonas con mayor potencialidad de anegamiento. Una multiplicación entre las diferentes coberturas y una reclasificación final permitió establecer el mapa de mayor probabilidad de anegamiento. Este último mostró una importante similitud con el mapa de áreas inundadas, por lo que puede ser considerado como una buena aproximación en la determinación de dichas áreas. Por último, se dan recomendaciones sobre las estaciones que deben instalarse para el monitoreo y prevención de este tipo de eventos en la zona.","palabras_clave":"Cuenca del río Quequén Grande|Inundación|Zonas de anegamiento|Peligrosidad de inundación|Metodología SIG|Llanura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62891,"idioma_id":4,"titulo":"k-domination for chordal graphs and related graph classes","resumen":"In this work we obtain a new graph class where the {k}-dominating function problem({k}-DOM) is NP-complete: the class of chordal graphs. We also identify somemaximal subclasses for which it is polynomial time solvable. Firstly, by relatingthis problem with the k-dominating function problem (k-DOM), we prove that{k}-DOM is polynomial time solvable for strongly chordal graphs. Besides, byexpressing the property involved in k-DOM in Counting Monadic Second-orderLogic, we obtain that both problems are linear time solvable for bounded tree-width graphs. Finally, we show that {k}-DOM is linear time solvable for spidergraphs.","palabras_clave":"{k}-dominating function, |$k$-dominating function,| computational complexity, |chordal graph"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62892,"idioma_id":4,"titulo":"First isolation of Leptospira interrogans from Lycalopex griseus (South American gray fox) in Argentina shows new MLVA genotype","resumen":"To identify carriers of Leptospira spp. in Argentina, we  trapped wild animals in Buenos Aires province  for three nights, capturing 12 Didelphis albiventris (white-eared  opossum), six Chaetophractus villosus (big hairy armadillo), five Lycalopex griseus (South  American gray fox) and two Conepatus chinga (Molina´s hog-nosed skunk). All  were tested by microscopic agglutination test, and five (two gray foxes, two armadillos, and one skunk) were positive for Leptospira  interrogans serovars Canícola, and Icterohaemorrhagiae, L. borgpetersenii serovar Castellonis,  and L. kirschneri serovar Grippotyphosa, at titers of 1:50 and  1:100. Kidneys from all animals were cultured, and one isolate of L. interrogans  from a gray fox was  obtained. Hamsters were inoculated with the isolate, and died after six days  with no macroscopic lesions at necropsy. However, histologic examination revealed  glomerulonephritis, interstitial nephritis, and pneumonia. The Leptospira strain from the South  American gray fox was analyzed serologically and its pathogenicity was established.  Genotyping through multiple-locus variable-number tandem repeat analysis (MLVA)  revealed that the strain belonged to a new genotype related to the L. interrogans serogroup  Icterohaemorrhagiae.","palabras_clave":"Genotype|Leptospira interrogans|Lycalopex griseus|multiple-locus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62893,"idioma_id":4,"titulo":"Effect of prolactin acting on the coeliac ganglion via the superior ovarian nerve on ovarian function in the postpartum lactating and non-lactating rat","resumen":"Whether prolactin (PRL) has a luteotrophic or luteolytic effect in the rat ovary depends on the nature of the corpora lutea present in the ovaries and the hormonal environment to which they are exposed. The aim  was to investigate the effect of PRL acting on the coeliac ganglion (CG) on the function of the corpora lutea on day 4 postpartum under either lactating or non-lactating conditions, using the CG?superior ovarian  nerve?ovary system. The ovarian release of progesterone (P), estradiol, PGF2a, and nitrites was assessed in the ovarian compartment at different incubation times. Luteal mRNA expression of 3b-HSD, 20a-HSD,  aromatase, PGF2a receptor, iNOS, Bcl-2, Bax, Fas and FasL was analysed in the corpus luteum of pregnancy at the end of the experiments. Comparative analysis of control groups showed that the ovarian release of P,  nitrites, and PGF2a, the expression of PGF2a receptor, and the Bcl-2\/Bax ratio were lower in non-lactating rats, with increased release of estradiol, and higher expression of aromatase, Fas and FasL, demonstrating the higher luteal functionality in ovaries of lactating animals. PRL added to the CG compartment increased the ovarian release of P, estradiol, nitrites and PGF2a, and decreased the Bcl-2\/Bax ratio in non-lactating rats; yet, with the exception of a reduction in the release of nitrites, such parameters were not modified in lactating animals. Together, these data suggest that the CG is able to respond to the effect of PRL and, via a neural pathway, fine-tune the physiology of the ovary under different hormonal conditions.","palabras_clave":"PROLACTIN|COELIAC GANGLION|SUPERIOR OVARIAN NERVE|PREGNANCY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62894,"idioma_id":4,"titulo":"Prenatal hyperandrogenism and lipid profile during different age stages: an experimental study","resumen":"OBJECTIVE:  The present study investigates the effect of prenatal hyperandrogenization on lipid metabolism and oxidant\/antioxidant balance.  DESIGN:  Experimental study.  SETTING:  Research institute.  ANIMAL(S):  Pregnant Sprague Dawley rats were subcutaneously injected with 2 mg free T between days 16 and 19 of pregnancy, and controls (C) received vehicle (0.1 mL of sesame oil). Prenatally hyperandrogenized female offspring (T2) had a condition that resembles polycystic ovary (PCO). Animals were weighed and killed at 21 and 60 days of age (N = 15 rats\/group).  INTERVENTION(S):  Ovarian tissue and truncal blood were obtained from the C and T2 groups.  MAIN OUTCOME MEASURE(S):  Circulating lipid profile (total cholesterol, high-density lipoprotein [HDL], low-density lipoprotein [LDL] cholesterol, and triglycerides) was quantified by colorimetric-enzymatic methods. Ovarian oxidative stress was evaluated by quantifying lipid peroxidation and glutathione content by spectofotometric assays. Ovarian fat content was evaluated by Red Oil staining and ovarian messenger RNA (mRNA) expression of peroxisome proliferator-activated receptor gamma (PPAR-&#947;) by real-time polymerase chain reaction (PCR).  RESULT(S):  At 60 days of age, 100% of group C rats and 20% of group T2 rats ovulated. At 21 days of age the T2 rats displayed lower body weight than C rats; however, at 60 days of age T2 and C rats showed similar body weights. The lipid profile (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides) was altered in the anovulatory and ovulatory phenotype of the T2 group, but the levels were higher in the anovulatory phenotype. Lipid peroxidation of rats at 21 and 60 days of age from T2 was similar to C but the antioxidant glutathione level was decreased in 21-day-old rats compared with C rats. The lipid content of ovarian tissue, determined by Red Oil staining, was higher in the T2 than in the C group. The mRNA expression of ovarian PPAR-&#947;, quantified by real time PCR, decreased in anovulatory rats at 60 days of age from T2 compared to C rats.  CONCLUSION(S):  Our findings reveal the importance of evaluating the complete lipid profile, especially at early stages of life after the prenatal hyperandrogenism condition. In addition, we demonstrated that the antioxidant-reduced glutathione would represent a good marker of oxidative stress as it is altered before lipid peroxidation. Prenatal hyperandrogenization also alters the gene expression of PPAR-&#947; in rats. Here we demonstrated for the first time that abnormalities in PPAR-&#947; and lipid profile were higher in rats showing an anovulatory phenotype than those displaying an ovulatory phenotype.","palabras_clave":"POLYCYSTIC OVARY SYNDROME|LIPID PROFILE|PPAR gamma"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62895,"idioma_id":4,"titulo":"Combined effects of leaf litter and soil microsite on decomposition process in arid rangelands.","resumen":"The objective of this study was to analyze the combined effects of leaf litter quality and soil properties on litter decomposition and soil nitrogen (N) mineralization at conserved (C) and disturbed by sheep grazing (D) vegetation states in arid rangelands of the Patagonian Monte. It was hypothesized that spatial differences in soil inorganic-N levels have larger impact on decomposition processes of non-recalcitrant than recalcitrant leaf litter (low and high concentration of secondary compounds, respectively). Leaf litter and upper soil were extracted from modal size plant patches (patch microsite) and the associated interpatch area (inter-patch microsite) in C and D. Leaf litter was pooled per vegetation state and soil was pooled combining vegetation state and microsite. Concentrations of N and secondary compounds in leaf litter and total and inorganic-N in soil were assessed at each pooled sample. Leaf litter decay and soil N mineralization at microsites of C and D were estimated in 160 microcosms incubated at field capacity (16 month). C soils had higher total N than D soils (0.58 and 0.41 mg\/g, respectively). Patch soil of C and interpatch soil of D exhibited the highest values of inorganic-N (8.8 and 8.4 mg\/g, respectively). Leaf litter of C was less recalcitrant and decomposed faster than that of D. Non-recalcitrant leaf litter decay and induced soil N mineralization had larger variation among microsites (coefficients of variation ¼ 25 and 41%, respectively) than recalcitrant leaf litter (coefficients of variation ¼ 12 and 32%, respectively). Changes in the canopy structure induced by grazing disturbance increased leaf litter recalcitrance, and reduced litter decay and soil N mineralization, independently of soil N levels. This highlights the importance of the combined effects of soil and leaf litter properties on N cycling probably with consequences for vegetation reestablishment and dynamics, rangeland resistance and resilience with implications for management and conservation. ","palabras_clave":"GRAZING DISTURBANCE|PERENNIAL GRASSES|NITROGEN|SHRUBS|SECONDARY COMPOUNDS|VEGETATION STATES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62896,"idioma_id":4,"titulo":"Artificial Neural Networks Applied to Statistical Design of Window Operators","resumen":"The design of binary W-operators, morphological operators that are translation-invariant and locally defined by a finite neighborhood window, corresponds to the problem of designing Boolean functions,  or their characteristic functions. One of the main issues regarding automatic design of W-operators, based on samples, is the one of generalization. Considering the designing of W-operators as a particular case of designing a pattern recognition system, in this paper we propose a new approach for the automatic design of binary W-operators. The approach consists on a functional representation of the class membership conditional probability for the whole set of patterns viewed through a given window, instead of generalizing the class labels (or the characteristic function values). The estimation of parameters for the functional representation uses a nonlinear regression performed by an artificial feed-forward neural network. The network training is  based on the weighted mean square error cost function, allowing us to use the marginal probability of each pattern viewed by a given window. Experimental results show that, for the applications presented in this  work, the proposed approach leads to better results than those from one of the best existing methods for generalization of characteristic functions of W-operators, like is the case of pyramidal multiresolution.","palabras_clave":"Image Processing|W-operators|Mathematical Morphology|Neural Networks"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62897,"idioma_id":4,"titulo":"Characterization of bio-dynamic speckles through classical and fuzzy mathematical morphology tools","resumen":"In this paper we characterize dynamic speckle signals, obtaining selective information  through the differentiation of morphological patterns of the temporal history of each pixel, using the  Morphological Granulometric Function. This method is applied to the analysis of images of apples and  corn seeds. Studies on the first ones were focused on the activity on their surface, related to healthy  and damaged areas, while for seeds on the viability of the embryo and endosperm. Subsequently, the  analysis was repeated using Fuzzy Mathematical Morphology techniques, comparing the results  obtained by both methods.","palabras_clave":"Dynamic Speckle|athematical Morphology|Fuzzy Mathematical Morphology|Morphological Granulometric Function"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62898,"idioma_id":4,"titulo":"Effectiveness evaluation of glyphosate oxidation employing the H2O2\/UVC process: toxicity assays with Vibrio fischeri and Rhinella arenarum tadpoles","resumen":"              The combination of hydrogen peroxide and UVC radiation (H2O2\/UVC)  is a simple and convenient process to decrease glyphosate concentration to  acceptable limits in water. In this work, the H2O2\/UVC process  was applied to the degradation of a commercial formulation of glyphosate in  water. Two types of microorganisms (Vibrio fischeri and Rhinella arenarum tadpoles) we used to investigate the toxicity of glyphosate at different times  (120, 240 and 360 min) of the H2O2\/UVC process without  removal of the concentration of H2O2 (M1, M2  and M3) and with H2O2 removed (M1,E,  M2,E and M3,E). Acetylcholinesterase  (AChE) and butyrylcholinesterase (BChE) activities were also measured in  tadpoles in order to  know possible sub-lethal effects. The initial concentration of glyphosate (50 mg a.e. L-1; M0) decreased to 27 mg a.e. L-1  over a reaction time of 120 min, to 11 mg a.e. L-1 at 240 min, and  to 4 mg a.e. L-1 after of 360 min. In V.  fischeri, M1,E causes 52% of inhibition, while  at the end of the photodegradation the inhibition represents only 17% (M3,E). Survival of  tadpoles was 100% in the samples M2, M3, and M1,E,  M2,E and M3,E. In tadpoles, the percentage of inhibition of AChE varied  from 21.3% for M1,E to 26.23% for M2,E and to 4,01% for M3,E;  while the percentage of inhibition of BChE varied from 25.05% for M1,E  to 27.89% for M2,E and to 1.86% for M3,E. Minor percentages of enzymatic inhibition were observed in samples without removal of H2O2:  13.96% (AChE) and 16% (BChE) for M2,  and 24.12 % (AChE) and 13.83 % (BChE) for M3.These results show the efficiency of the H2O2\/UVC process to reduce the toxicity of water  or wastewaters polluted by commercial formulations of  glyphosate. The  application of biological assays is important to evaluate the AOP processes and  permit to determinate the final point of the treatment.  According to the ecotoxicity assays the conditions corresponding to M2  (11 mg a.e. L-1 of glyphosate and 4 mg L-1 of H2O2)  could be the final point of the treatment with the H2O2\/UV process.&lt;\/body&gt;  &lt;\/html      ","palabras_clave":"Vibrio fischeri |R. arenarum|glyphosate"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62899,"idioma_id":4,"titulo":"Reproductive activity of anurans in a dominant agricultural landscape from central-eastern Argentina","resumen":"Agriculture can modify natural systems through habitat loss and fragmentation, as well as through the effect of agrochemicals on biological traits such as reproduction. We studied anuran diversity and reproduction of assemblages from 3 sites with different degrees of agricultural activities (an agroecosystem, a transitional area, and a natural forest) located in Entre Ríos province (Argentina). We conducted several field surveys during 2 soybeancropping periods from November 2006 to April 2008. A total of 23 anuran species were identified. Richness, evennessand diversity differed among sites. No range-abundance models were statistically adequate to describe the distribution of abundances within the agroecosystem, whereas the anuran species from the other 2 sites fitted the normal logarithmic model. The location and substrates used for nuptial calling in ponds, as well as the reproductive periods of anuran species varied among sites. These findings might respond to a combination of factors, such as anuran composition,species abundances, availability of reproductive microhabitats, and variation of microclimatic characteristics among sites, the agroecosystem being the most different anuran reproductive community. Finally, our results suggest that agricultural land use have adverse effects on the reproduction of anurans in central-eastern Argentina.","palabras_clave":"BREEDING ACTIVITY|AMPHIBIANS|AGROECOSYSTEM|ENTRE RIOS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62900,"idioma_id":4,"titulo":"An ultrasonic-accelerated oxidation method for determining the oxidative stability of biodiesel","resumen":"Biodiesel is considered an alternative energy because it is produced from fats and vegetable oils by means  of transesterification. Furthermore, it consists of fatty acid alkyl esters (FAAS) which have a great influence  on biodiesel fuel properties and in the storage lifetime of biodiesel itself. The biodiesel storage stability  is directly related to the oxidative stability parameter (Induction Time ? IT) which is determined by  means of the Rancimat method. This method uses condutimetric monitoring and induces the degradation  of FAAS by heating the sample at a constant temperature. The European Committee for Standardization  established a standard (EN 14214) to determine the oxidative stability of biodiesel, which requires it  to reach a minimum induction period of 6 h as tested by Rancimat method at 110 C. In this research, we  aimed at developing a fast and simple alternative method to determine the induction time (IT) based on  the FAAS ultrasonic-accelerated oxidation. The sonodegradation of biodiesel samples was induced by  means of an ultrasonic homogenizer fitted with an immersible horn at 480 Watts of power and 20 duty  cycles. The UV?Vis spectrometry was used to monitor the FAAS sonodegradation by measuring the absorbance  at 270 nm every 2. Biodiesel samples from different feedstock were studied in this work. In all  cases, IT was established as the inflection point of the absorbance versus time curve. The induction time  values of all biodiesel samples determined using the proposed method was in accordance with those  measured through the Rancimat reference method by showing a R2 = 0.998.","palabras_clave":"Biodiesel|Sondegradation|Experimental design|Oxidative stability|Fatty acid alkyl esters|UV-Vis spectrophotometry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62901,"idioma_id":4,"titulo":"Action-verb processing in Parkinson's disease: new pathways for motor-language coupling","resumen":"Recent studies suggest that action-verb processing is particularly affected in early stage Parkinson´s disease (PD), highlighting the potential role of subcortical areas in language processing and in the semantic integration of actions. However, this disorder-related language impairment is frequently unrecognized by clinicians and often remains untreated. Early detection of action-language processing deficits could be critical for diagnosing and developing treatment strategies for PD. In this article, we review how action-verb processing is affected in PD and propose a model in which multiple and parallel frontotemporal circuits between the cortex and the basal ganglia provide the anatomic substrate for supporting action-language processing. We hypothesize that contextual coupling of action-language networks are partially dependent on cortical-subcortical integration, and not only on somatotopic motor cortical organization or in a mirror neuron system. This hypothesis is supported by both experimental and clinical evidence. Then, we identify further research steps that would help to determine the reliability of action-language impairments as an early marker of PD. Finally, theoretical implications for clinical assessment and for models of action-language interaction (action-perception cycle theories, mirror system models of language, and embodied cognition approaches to language) are discussed.","palabras_clave":"PARKINSON_DISEASE|ACTION-VERB_PROCESSING|BASAL_GANGLIA|MOTOR_LANGUAGE_COUPLING|MIRROR_NEURONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62902,"idioma_id":4,"titulo":"Reassignment of Lioestheria codoensis cardoso (Spinicaudata - Anthronestheriidae) from the Lower Cretaceous of Brazil: systematics and paleoecology","resumen":" Lioestheria codoensis Cardoso was described from materials originally collected from the Codó Formation (Lower Cretaceous, Parnaíba Basin, Piauí State), northeastern Brazil. Since its discovery L. codoensis has been assigned to different genera by different authors [as Estheriina (Nudusia) and Cyzicus (Lioestheria)]. In this paper, previous systematic assignments are reviewed and discussed in light of new scanning electron microscopy studies of new material. As a result of this research, the species is assigned to a new genus of the Family Antronestheriidae, and the study integrates palaeontological, biological and ecological approaches to provide a robust description for the species. Previous assignments to other genera (Estheriina, Lioestheria and Cyzicus) are also assessed and comparisons with other related genera of the Family Anthronestheriidae(Pseudestherites, Etmosestheria, Anthronestheria and Paleolepthestheria? chinensis) are made. Detailed stratigraphical (Codó, Santana, Itapecuru, Rio da Batateira and Missão Velha formations) and geographical (seven localities) records are compiled. Finally, the autecology and within-species morphological variation are also addressed.","palabras_clave":"Anthronestheriidae|Santana Formation|Early Cretaceous|Brazil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62903,"idioma_id":4,"titulo":"Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat","resumen":"Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a welldocumented phenomenon and it has been shown through transcriptome analysis thatgenes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis,participate in the process. Thus, we investigated the influence of post-transcriptionalsilencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somaticembryogenesis in wheat. We found that PTGS of either of the target genes drasticallyinhibits callus regeneration and overall efficiency of transformation, in a similar manneras the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the mediumwith glutathione did not overcome the observed low efficiency of wheat transformation.Furthermore, of the small number of obtained transformants, none exhibited alteredGSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential forsomatic embryogenesis and, as a consequence, it is difficult to regenerate wheatplants with silenced GSH1 and GSH2 genes.","palabras_clave":"Wheat|somatic embryogenesis|glutathione"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62904,"idioma_id":4,"titulo":"High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus","resumen":"Delphacodes kuscheli Fenah (Hemiptera: Delphacidae) is the main natural vector of Mal de Río Cuarto virus (MRCV) (Reoviridae: Fijivirus), which infects different poaceae and causes the most important maize disease in Argentina. Wheat is a useful host to study virus-vector interactions. Under experimental conditions, less than 50% of D. kuscheli planthoppers fed on a MRCV-infected plant can transmit the virus. This fact is influenced by insect development stage at acquisition and the latency period. This work describes the relation between transmission efficiency and MRCV accumulation in its planthopper vector. First and 3rd D. kuscheli instars nymphs were allowed to feed on MRCV-infected plants and 9 or 17 days after the acquisition access period (AAP), viral load of transmitting and non-transmitting planthoppers was quantified by qPCR. The transmitting planthoppers showed significantly higher viral titers than non-transmitting ones, suggesting that successful transmission is positively associated to viral accumulation in the insect. However, planthoppers of the 3rd instars group did not transmit the virus 9 days after AAP, even when 46% had similar titers to the transmitting insects of the other treatments. These results indicate that additional factors influence MRCV transmission efficiency when acquisition occurs in older planthoppers. This is the first precise quantitative analysis of MRCV in its main vector species and will definitely contribute to better understand planthopper-Fijivirus interactions and its epidemiological implications.","palabras_clave":"vectorÐvirus interaction|Fijivirus|persistent propagative transmission"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62905,"idioma_id":4,"titulo":"The catechin flavonoid reduces proliferation  and induces apoptosis of murine lymphoma cells LB02 through modulation of antiapoptotic proteins.","resumen":"Received 30 Jul 2012Accepted 18 Jan 2013Revista Brasileira de FarmacognosiaBrazilian Journal of Pharmacognosy The catechin fl avonoid reduces proliferationAbstract: Flavonoids are products of secondary metabolism of plants. They arepresent in herbs and trees and also act as natural chemopreventives and anticanceragents. Ligaria cuneifolia (Ruiz & Pav.) Tiegh., Loranthaceae, is a hemiparasitespecies that belongs to Argentine flora. Phytochemical studies have disclosed thepresence of quercetin, catechin-4&#946;-ol and pro-anthocyanidine as polyphenoliccompounds in the active extracts. We previously demonstrated that ethyl acetateextract was capable of reducing cell proliferation and inducing apoptotic death oflymphoid tumor cells. The aim of the current study is to determine whether or notcatechin, isolated from L. cuneifolia extracts can induce leukemia cell death and todetermine its effect on the cytoplasmatic proteins that modulate cell survival. Ourresults show that catechin can reduce proliferation of murine lymphoma cell lineLB02. The effect is mediated by apoptosis at concentrations upper to 100 &#956;g\/mL.Cell death is related to the loss of mitochondrial membrane potential (&#916;&#936;m) and adown regulation of survivin and Bcl-2 together with the increase of pro-apoptoticprotein Bax. In summary, the current study indicates that catechin present in theextract of L. cuneifolia is in part, responsible for the anti-proliferative activity ofwhole extracts by induction of &#916;&#936;m disruption and modulation of the anti-apoptoticproteins over expressed in tumor cells. These results give new findings into thepotential anticancer and chemopreventive activities of L. cuneifolia.Keywords:apoptosisBcl-2\/Bax ratiocatechinleukemiamitochondrial membrane potentialsurvivinIntroductionFlavonoids, polyphenolic compounds presentin the plant extracts from the heterogeneous family,are functionally related. Plants containing fl avonoidderivatives have been found to be active as a diseasepreventive and therapeutic agent, present in folk medicinepreparations that have been used for thousands of years.They are the main components in a healthy diet rich infruits and vegetables and are concentrated especially intea, apples and grapes (Havsteen, 2002; Chung et al.,2003).As natural products, they contain a wide rangeof biochemical and pharmacological properties; themost investigated of which being their cancer preventiveactivities (Middleton et al., 2000; Sporn & Suh, 2002;Aop02213#Both authors contributed equally","palabras_clave":"APOPTOSIS|BCL-2\/BAX RATIO|CATECHIN|LEUKEMIA|MITOCHONDRIAL MEMBRANE POTENTIAL|SURVIVIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62906,"idioma_id":4,"titulo":"VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display","resumen":"The Foot-and-mouth desease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.","palabras_clave":"Foot-and-mouth disease virus |Baculovirus display |VP1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62907,"idioma_id":4,"titulo":"Improvement of functional and antimicrobial properties of brewery byproduct hydrolysed enzymatically","resumen":"The aim of this study is to investigate the characteristics of brewer?s spent grain (BSG) hydrolysates. Hydrolysis was performed using a Bacillus cereus sp. extracellular peptidase. The proteins were hydrolysed for 2, 8 and 24 h, achieving degree of hydrolysis ranging from 2.65 to 21.80%. During enzymatic hydrolysis, average peptide chain length decreased rapidly and the soluble forms increased. Solubility of the hydrolysates showed a good correlation with turbidity. The water\/oil holding capacity, the emulsifying properties and the foaming expansion were analysed, and improved functional properties were found respect to the control. For the studied hydrolysates concentrations no gel formation were obtained. However, BSG hydrolysates exhibited also desirable rheological properties making their good candidate for many food formulations. Thermal characterization revealed the energy recovery by the enzymatic hydrolysis process. The understanding of hydrolysates antimicrobial properties may lead to utilize their as potent natural antimicrobial against Escherichia coli O157:H7. In the light of the results, hydrolysates made from BSG can be converted into a high value protein food ingredient.","palabras_clave":"ENZYMATIC HYDROLISIS|ANTIMICROBIAL ACTIVITY|BREWER´S SPENT GRAIN HYDROLYSATES|FUNCTIONAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62908,"idioma_id":4,"titulo":"Biochemical characterization and thermal inactivation of polyphenol oxidase from radish (Raphanus sativus var. sativus)","resumen":"Polyphenoloxidase (PPO) is the target for the development of several food antibrowning agents. Different substrates (pyrocatechol, gallic acid, chlorogenic acid, caffeic acid, 3,4 dihydroxybenzoic acid, p-cumaric acid, L-tyrosine, pyrogallic acid and phloroglucinol) were analyzed to determine their affinities with radish PPO. Pyrocatechol, gallic acid and pyrogallic acid were the substrates that showed high affinity based on Vmax \/KM ratio. The optimum pH for the PPO using these three substrates were pH = 7 and the optimum temperatures were 20, 60 and 20-40 ºC for pyrogallic acid, gallic acid and pyrocatechol, respectively. The kinetics of thermal inactivation was successfully modeled by a biphasic model (r2 > 0.888), attributed to the presence of two enzyme fractions, a heat-labile easily inactivated even at low blanching temperatures, and a heat-resistant fraction that requires blanching temperatures above 80ºC to reach 70% of inactivation. The kinetics constants of this model for both heat-labile and heat-resistant increased with temperature in the range from 60 to 90 ºC. The activation energy ratio of resistant to labile fraction was found to be 6 (EaL= 142 kJ\/mol).","palabras_clave":"ENZYMATIC KINETICS|THERMAL STABILITY|MINIMALLY PROCESSING|POLYPHENOLOXIDASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62909,"idioma_id":4,"titulo":"Optimization of chitosan treatments for managing microflora in lettuce seeds without affecting germination","resumen":"Many studies have focused on seed decontamination but no one has been capable of eliminating all pathogenic bacteria. Two objectives were followed. First, to assess the in vitro antimicrobial activity of  chitosan against: (a) Escherichia coli O157:H7, (b) native microflora of lettuce and (c) native microflora of lettuce seeds. Second, to evaluate the efficiency of chitosan on reducing microflora on lettuce seeds.  The overall goal was to find a combination of contact time and chitosan concentration that reduces the microflora of lettuce seeds, without affecting germination. After treatment lettuce seeds presented no  detectable microbial counts (<102 CFU\/50 seeds) for all populations. Moreover, chitosan eliminated E. coli.  Regardless of the reduction in the microbial load, a 90% reduction on germination makes imbibition with chitosan, uneconomical. Subsequent treatments identified the optimal treatment as 10 min contact with  a 10 g\/L chitosan solution, which maintained the highest germination percentage.  © 2012 Published by Elsevier Ltd.","palabras_clave":"BIOPRESERVATIVES|E.COLI|ORGANIC|PREHARVEST"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62910,"idioma_id":4,"titulo":"Application of tea tree and clove essential oil on late development stages of Butterhead lettuce: Impact on microbiological quality","resumen":"Two essential oils (tea tree and clove) were applied as preharvest sanitizers on late development stages of Butterhead lettuce and its effect during postharvest storage was analyzed. The essential oils were applied in vitro (3 MIC) and proved to be effective to inhibit native microflora of Butterhead lettuce. In vivo, the essential oils were applied in a single dose (14, 10, 7, 3 and 0 days before harvest) and in successive applications. Both essential oils showed significant reductions in mesophyllic bacteria and total coliforms when applied at harvest and in successive applications. Clove showed no significant reductions in psychrophilic, yeast and molds counts. The sensorial quality of the product was assessed by a panel of trained judges. No significant differences were found between control and treated samples. After 5 days of refrigerated storage, lettuce treated with tea tree was able to reduce microbial counts after storage (2 log) without affecting sensorial characteristics. Application of clove resulted in no significant reduction on postharvest microbial counts. These results may indicate absence of long term antimicrobial activity of clove on the native microflora of Butterhead lettuce.","palabras_clave":"PREHARVEST|POSTHARVEST|ORGANIC|GREENHOUSE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62911,"idioma_id":4,"titulo":"Morphological, physicochemical and sensory evaluation of celery harvested from early to late maturity","resumen":"236 Hortic. bras., v. 31, n. 2, abr. - jun. 2013  Celery is a highly appreciated  vegetable for its fibber content  and nutritional value. The plant has  approximately 20 leaves attached to  the crown, were the stem is. Each leaf  is formed by a large and fully solid  petiole and several leaflets. The petiole  is the most commonly used part for  consumption.  High quality celery consists of stalks  which are well formed, have thick  petioles, are compact, have minimal  petiole twisting, and have a light green  and fresh appearance. Additional quality  indexes are stalk and midrib length,  absence of defects such as blackheart,  pithy petioles, seed stalks, cracks or  splits, and absence of insect damage  YOMMI AK; DI GERÓNIMO NM; CARROZZI LE; QUILLEHAUQUY V; GOÑI MG; ROURA SI. 2013. Morphological, physicochemical and sensory  evaluation of celery harvested from early to late maturity. Horticultura Brasileira 31: 236-241.  Morphological, physicochemical and sensory evaluation of celery  harvested from early to late maturity  Alejandra Karina Yommi1; Natalia Marcela Di Gerónimo1; Liliana Esther Carrozzi1; Victoria  Quillehauquy1; María Gabriela Goñi2,3; Sara Inés Roura2,3  1UIB (Unidad Integrada Balcarce), Postcosecha y Calidad de Frutas y Hortalizas, CC 276, 7620 Balcarce, Argentina; ayommi@balcarce.  inta.gov.ar; natydiger@hotmail.com; lcarrozzi@balcarce.inta.gov.ar; vquillehauquy@balcarce.inta.gov.ar 2CONICET, Buenos Aires,  Argentina; 3FIA-UNMdP, Grupo de Investigación en Ingeniería en Alimentos, Juan B Justo 4302, 7600 Mar del Plata, Argentina; ggoni@  fi.mdp.edu.ar; sroura@fi.mdp.edu.ar  ABSTRACT  The aim of this study was to determine quality changes in  petioles of self-blanching celery (Apium graveolens), cv. Golden  Boy, harvested at 80, 87, 94, 101, 108, 115, 122 and 129 days aftertransplanting  (DAT). Total weight (TW), total length (TL), number  of leaves per plant (LN) and by plant zone (external, LNZE; middle,  LNZM; internal, LNZI), and petiole length (PL) were evaluated at  each harvest time. Petioles quality of each zone in the plant were  analyzed in terms of: color (hue angle), texture (cutting force), total  soluble solids content (TSS) and titratable acidity (TA). The petioles  also were sensorially evaluated by descriptive analysis considering  visual characteristics (flexibility, hollowness), flavor (typical flavor  and odor) and texture attributes (hardness, crunchiness, juiciness and  fibrousness). From 80 to 129 DAT, TW and TL increased 33 g\/DAT  and 0.62 cm\/DAT, respectively. Leaves development was detected  until the 122 DAT; LNZI grew to a greater extent. The PL increased  during plant development, mainly in middle and internal leaves;  being the middle leaves the longest. Hue angle and cutting force were  similar in external and middle petioles and both resulted higher than  internal ones until the 122 and 115 DAT, respectively. The juiciness  increased and flexibility decreased during maturation, resulting  108 DAT maturity stage less flexible and juicier than 80 DAT. No  significant differences between harvesting dates were observed in  the other sensory properties evaluated. Inflorescence differentiation  was detected at 115 DAT, causing an increase in texture, TSS and  TA. Optimum yield and quality balance were achieved at 122 DAT.  Delayed harvest is associated with higher plants but also with lower  petioles quality, mainly due to an undesirable change in texture.","palabras_clave":"Apium graveolens|bolting|madurez |texture"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62912,"idioma_id":4,"titulo":"Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment","resumen":"                      A keratinase isolated from Paecilomyces  lilacinus (LPS #876) was tested against proteins present in the skin such  as albumin, collagen, elastin and keratin. The enzyme was able to act on all  assayed substrates but collagen was hydrolyzed faster. Keratinase was  physically immobilized onto PVA-pectin cryogels and enzyme release was  20.8±2.1, 63.8±0.2, 41.5±3.5 and 26.0±3.5 % in cryogels containing pectins with  esterification degrees (DE) 33.0, 55.0, 62.7 and 71.7 % respectively at 37 ºC  after 3 hours incubation. In presence of 0.75M NaCl, the percentage of enzyme  release increased to: 57.5±1.5, 65.8±3.8, 57.3±0.2 and 34.0±4.0 for the four  pectins respectively.   In-vitro studies of enrofloxacin release from PVA-pectin cryogels  at pH close to the human skin (pH= 5.5) showed 15.0 % free antibiotic following  first order kinetic at 37ºC after 5 h incubation. However, in the presence of  keratinase only 6.0 % of enrofloxacin was released under the same experimental  conditions.","palabras_clave":"PVA CRYOGELS  Wound healing|KERATINASE|ENROFLOXACIN|CONTROLLED RELEASE|WOUND HEALING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62913,"idioma_id":4,"titulo":"Influence of the synthesis conditions on the physicochemical properties and acidity of Al-MCM-41 as catalysts for the cyclohexanone oxime rearrangement","resumen":"Al-containing  mesoporous catalysts with MCM-41 structure and high surface area have been  successfully prepared by direct hydrothermal synthesis. Various  characterization techniques including XRD, N2 adsorption, ICP-OES, NMR, FT-IR and adsorption of pyridine coupled  to FT-IR spectroscopy were employed. A detailed study about the relationships  existing between the different synthesis parameters, the physico-chemical  properties, the acid behaviour and the catalytic performance is presented in this contribution. We  have suggested that the active sites for the Beckmann rearrangement of  cyclohexanone oxime are nest silanols (at structural defects) of very weak Bronsted acid character, which is  associated with the introduction of Al into the mesoporous framework. In all  the cases, employing soft reaction conditions, we have obtained selectivity to  caprolactam of 100%. Finally, a Si\/Al initial molar ratio of 20, a stirring of synthesis  gel for 7 h and a hydrothermal treatment of 6 days seem to be the optimum  synthesis conditions to obtain the highest framework Al incorporation, more abundant  weak acid sites (silanol nests) and maximum yield for caprolactam.","palabras_clave":"MESOPOROUS MATERIALS|Al-MCM-41|SYNTHESIS TIME|GEL STIRRING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62914,"idioma_id":4,"titulo":"Monounsatured fatty acid aerobic synthesis in Bradyrhizobium TAL 1000 peanut-nodulating in affected by temperature","resumen":"Abstract  Aims: The aim of this work was to clarify the mechanism of monounsaturated  fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high  temperature on this process.  Methods and Results: Bradyrhizobium TAL1000 was exposed to a high growth  temperature and heat shock, and fatty acid composition and synthesis were  tested. To determine the presence of a possible desaturase, a gene was identify  and overexpressed in Escherichia coli. The desaturase expression was detected  by RT-PCR and Western blotting. In B. TAL1000, an aerobic mechanism for  MUFA synthesis was detected. Desaturation was decreased by high growth  temperature and by heat shock. Two hours of exposure to 37°C were required  for the change in MUFA levels. A potential &#916;9 desaturase gene was identified  and successfully expressed in E. coli. A high growth temperature and not heat  shock reduced transcript and protein desaturase levels in rhizobial strain.  Conclusions: In B. TAL1000, the anaerobic MUFA biosynthetic pathway is  supplemented by an aerobic mechanism mediated by desaturase and is downregulated  by temperature to maintain membrane fluidity under stressful  conditions.  Significance and Impact of the Study: This knowledge will be useful for  developing strategies to improve a sustainable practice of this bacterium under  stress and to enhance the bioprocess for the inoculants? manufacture.","palabras_clave":"FATTY ACID|RHIZOBIA|TEMPERATURE|DESATURASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62915,"idioma_id":4,"titulo":"Hydrogen Embrittlement of Magnesium and Magnesium Alloys: A Review","resumen":"Magnesium and magnesium alloys are susceptible to stress corrosion cracking in various environments, including distilled water. There is compelling evidence to conclude that SCC is assisted, at least in part, by hydrogen embrittlement. This paper reviews the thermodynamics of the Mg-H system and the kinetics of hydrogen transport. Aspects of magnesium corrosion relevant to hydrogen absorption are also discussed. Crack growth mechanisms based on delayed hydride cracking, hydrogen adsorption dislocation emission, hydrogen enhanced decohesion, and hydrogen enhanced localized plasticity have been proposed and evidence for each of them is reviewed herein.","palabras_clave":"MAGNESIUM ALLOYS|HYDROGEN EMBRITTLEMENT|STRESS CORROSION CRACKING|MECHANISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62916,"idioma_id":4,"titulo":"Plasma Membrane Calcium ATPase Activity is Regulated by Actin Oligomers Through Direct Interaction","resumen":"SUMMARY As recently described by our group, Plasma Membrane Calcium ATPase (PMCA) activity can be regulated by the actin cytoskeleton. In this study, we characterize the interaction of purified G-actin with isolated PMCA and examine the effect of G-actin during the first polymerization steps. As measured by surface plasmon resonance (SPR) G-actin directly interacts with PMCA with an apparent 1:1 stoichiometry in the presence of Ca2+ with an apparent affinity in the micromolar range. As assessed by the photoactivatable probe [125I]TID-PC\/16, the association of PMCA to actin produced a shift in the distribution of the conformers of the pump towards a calmodulin-activated conformation. G-actin stimulates Ca2+-ATPase activity of the enzyme when incubated under polymerizing conditions displaying a cooperative behavior. The increase in the Ca2+-ATPase activity was related to an increase in the apparent affinity for Ca2+ and an increase in the phosphoenzyme levels at steady-state. Although SPR experiments revealed only one binding site for G-actin, results clearly indicate that more than one molecule of G-actin was needed for a regulatory effect on the pump. Polymerization studies showed that the experimental conditions are compatible with the presence of actin in the first stages of assembly. Altogether, these observations suggest that the stimulatory effect is exerted by short oligomers of actin. The functional interaction between actin oligomers and PMCA represents a novel regulatory pathway by which the cortical actin cytoskeleton participates in the regulation of cytosolic Ca2+ homeostasis.","palabras_clave":"ACTIN |OLIGOMERS|PMCA|REGULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62917,"idioma_id":4,"titulo":"Oxyanions as inhibitors of chloride-induced crevice corrosion of Alloy 22","resumen":"Oxyanions were tested as inhibitors of the chloride-induced crevice   corrosion of Alloy 22, at 90&nbsp;°C. Nitrate was the most efficient   inhibitor showing RCRIT&nbsp;=&nbsp;0.2 for the two tested chloride concentrations. Sulphate showed RCRIT values of 1 and 2 in 0.1&nbsp;mol\/L and 1&nbsp;mol\/L NaCl solutions, respectively. Carbonate showed RCRIT&nbsp;=&nbsp;1 while bicarbonate and carbonic acid did not show any inhibiting effect. Chromate and molybdate showed RCRIT&nbsp;=&nbsp;0.5   in 0.1&nbsp;mol\/L NaCl solutions being less effective in 1&nbsp;mol\/L NaCl   solutions. Tungstate produced a repassivation potential increase without   reaching a complete inhibition.","palabras_clave":"ALLOY|POLARIZATION|CREVICE CORROSION|REPASSIVATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62918,"idioma_id":4,"titulo":"Kinetic study of benzyl sulfamide synthesis by thermolysis of N-(benzyl)-N´-(tert-butoxycarbolyl) sulfamide","resumen":"In this investigation, a kinetic study of the thermolysis of N&#8208;(benzyl)&#8208;N´&#8208;(tert&#8208;butoxycarbonyl)sulfamide to yield benzylsulfamide in an efficient manner was performed. The thermolysis reaction was monitored in helium flow by thermogravimetry at different heating rates 0.2 and 10 oC\/min. The activation energy value was obtained from the Kissinger&#8208;Akahira&#8208;Sunose isoconversional method and theoretical calculations (from Transition State Theory). The reaction model of the process was studied by means of the master&#8208;plot method.Results obtained from experiments of thermolysis performed under the melting point temperature of N&#8208;(benzyl)&#8208;N´&#8208;(tert&#8208;butoxycarbonyl) sulfamide fit with an Avrami&#8208;Erofeev model whereas data found for experiments at higher temperatures fit with first order model.Isothermal experiments were simulated at 115, 120 and 130 oC using the model&#8208;free method, employing only the activation energy value.","palabras_clave":"SULFAMIDES|KINETIC STUDY|BENZYLSULFAMIDE|MODEL-FREE METHOD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62919,"idioma_id":5,"titulo":"El sitio Ampajango, cincuenta años después","resumen":"En este trabajo se abordan los aspectos generales de la explotación del sitio cantera-taller Ampajango,indagando sobre su rol en la definición de la base regional de recursos líticos del Valle de Yocavil. Este sitio  fue analizado previamente por Cigliano y presentado como un caso de ocupación de cazadores-recolectores.  A través de nuestro trabajo de campo delimitamos la superficie del sitio y recolectamos material de superficie.  Los estudios tecnológicos se centraron en la descripción de las actividades de producción lítica. En el sitio Ampajango se desarrollaron desde las primeras actividades de la secuencia de producción (extracción  de formas base, preparación de bifaces) hasta la formatización y regularización de filos. Proponemos que el sitio Ampajango habría funcionado como un sitio cantera-taller sólo en momentos previos al desarrollo de sociedades agro-alfareras. Posteriormente, se construyeron y se superpusieron otros paisajes lo cual indica una concepción distinta del espacio.","palabras_clave":"VALLE DE YOCAVIL|RECURSOS LITICOS|SITIO CANTERA-TALLER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62920,"idioma_id":5,"titulo":"SISTEMA DE PRONOSTICO EXPERIMENTAL EN ALTA RESOLUCION CON  EL MODELO BRAMS","resumen":"Este trabajo presenta las caractersticas del sistema de pronostico numerico en alta  resolucion basado en el modelo BRAMS en su version 4.2, dise~nado para el pronostico  de tormentas en la region pampeana y que se procesa en forma experimental en  el Servicio Meteorologico Nacional (SMN). Se realiza una descripcion del sistema  de pronostico mostrando un caso de estudio y una primera evaluacion de la calidad  del mismo. En particular, se presentan los resultados de la vericacion operativa de  los pronosticos aplicando metodologas adecuadas para pronosticos en alta resoluci  on. Se hace hincapie en la necesidad de utilizar informacion provista por sensores  remotos que cuenten con una mayor resolucion espacial y temporal que las utilizadas  habitualmente. Asimismo, se muestran los resultados de la aplicacion de la  metodologa de los pronosticos probabilsticos a la precipitacion y se examinan las  ventajas de su utilizacion dada la incorporacion de la incertidumbre asociada a las  simulaciones numericas de la atmosfera.","palabras_clave":"PRONOSTICO PRECIPITACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62921,"idioma_id":4,"titulo":"Functional capacity of Shiga-toxin promoter sequences in eukaryotic cells","resumen":"Shiga toxins (Stx) are the main virulence factors in enterohemorrhagic Escherichia coli (EHEC) infections, causing diarrhea and hemolytic uremic syndrome (HUS). The genes encoding for Shiga toxin-2 (Stx2) are located in a bacteriophage. The toxin is formed by a single A subunit and five B subunits, each of which has its own promoter sequence. We have previously reported the expression of the B subunit within the eukaryotic environment, probably driven by their own promoter. The aim of this work was to evaluate the ability of the eukaryotic machinery to recognize stx2 sequences as eukaryotic-like promoters. Vero cells were transfected with a plasmid encoding Stx2 under its own promoter. The cytotoxic effect on these cells was similar to that observed upon incubation with purified Stx2. In addition, we showed that Stx2 expression in Stx2-insensitive BHK eukaryotic cells induced drastic morphological and cytoskeletal changes. In order to directly evaluate the capacity of the wild promoter sequences of the A and B subunits to drive protein expression in mammalian cells, GFP was cloned under eukaryotic-like putative promoter sequences. GFP expression was observed in 293T cells transfected with these constructions. These results show a novel and alternative way to synthesize Stx2 that could contribute to the global understanding of EHEC infections with immediate impact on the development of treatments or vaccines against HUS","palabras_clave":"Stx|PROMOTOR|EUCARIOTAS|PLASMIDO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62922,"idioma_id":4,"titulo":"Interleukin-10 controls human peripheral PMN activation triggered by lipopolysaccharide","resumen":"Large amounts of anti-inflammatory mediators, such as interleukin (IL)-10, are produced and found earlyin the course of sepsis. We explore the role of IL-10 on neutrophil (PMN) activation\/function using anin vitro model. Isolated human PMN were pre-incubated with lipopolysaccharide (LPS) and\/or IL-10 for 18 h. Subsequently, a second LPS exposure was performed and CD11b and CD66b up-regulation, andthe reactive oxygen species (ROS) generation were measured 2 h later. We found that IL-10 preventedPMN activation and the secretion of TNF-a and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposure18 h. Subsequently, a second LPS exposure was performed and CD11b and CD66b up-regulation, andthe reactive oxygen species (ROS) generation were measured 2 h later. We found that IL-10 preventedPMN activation and the secretion of TNF-a and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposure18 h. Subsequently, a second LPS exposure was performed and CD11b and CD66b up-regulation, andthe reactive oxygen species (ROS) generation were measured 2 h later. We found that IL-10 preventedPMN activation and the secretion of TNF-a and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposuremodel. Isolated human PMN were pre-incubated with lipopolysaccharide (LPS) and\/or IL-10 for 18 h. Subsequently, a second LPS exposure was performed and CD11b and CD66b up-regulation, andthe reactive oxygen species (ROS) generation were measured 2 h later. We found that IL-10 preventedPMN activation and the secretion of TNF-a and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposureIL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurea and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generationwas highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurepossible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposurea or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrineactivating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposure","palabras_clave":"LPS, Neutrophils, IL-10"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62923,"idioma_id":4,"titulo":"Effects  of an Inhibitor of the Gamma-Secretase Complex on Proliferation and Apoptotic Parameters in a FOXL2-Mutated Granulosa Tumor Cell Line (KGN)","resumen":"Ovarian granulosa cell tumors (GCTs) represent 3-5% of all ovarian malignancies.Treatments have limited proven efficacy and biologically targeted treatment is lacking. The aim ofthis study was to investigate the role of Notch signaling in the proliferation, steroidogenesis,apoptosis, and PI3K\/AKT pathway in a FOXL2-mutated granulosa tumor cell line (KGN),representative of the adult form of GCTs. When Notch signaling is initiated, the receptors expose acleavage site in the extracellular domain to the metalloproteinase TACE and, following thiscleavage, Notch undergoes another cleavage mediated by the presenilin-&#947;-secretase complex. Toachieve our goal, DAPT, an inhibitor of the &#61543;-secretase complex, was used to investigate the role ofthe Notch system in parameters associated with cell growth and death, using a human granulosacell tumor line (KGN) as an experimental model. We observed that JAGGED1, DLL4, NOTCH1and NOTCH4 were highly expressed in KGN cells as compared to granulosa-lutein cells obtainedfrom ART patients. The proliferation and viability of KGN cells, as well as progesterone andestradiol production, decreased in the presence of 20 &#956;M DAPT. Apoptotic parameters like PARPand caspase 8 cleavages, BAX and BCLXshort increased in KGN cells cultured with DAPT, whileothers such as BCL2, BCLXlong, FAS and FASL did not change. AKT phosphorylation decreasedand PTEN protein increased when Notch signaling was inhibited in KGN cells. We conclude thatthe Notch system acts as a survival pathway in KGN cells, and might be interacting with thePI3K\/AKT pathway.","palabras_clave":"NOTCH|KGN|APOPTOSIS|PI3K\/AKT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62924,"idioma_id":4,"titulo":"Sublethal effects of Leptolegnia chapmanii (Straminipila: Peronosporomycetes) on some biological parameters of the dengue vector, Aedes aegypti","resumen":"Aedes aegypti (L.) is the primary vector of dengue in the Americas. The use of chemical insecticides is recommended during outbreaks of dengue to reduce the number of adult mosquitoes, but because Ae. aegypti is highly synanthropic, the use of insecticides in densely populated areas is a dangerous practice. Leptolegnia chapmanii Seymour (Straminipila: Peronosporomycetes) is an entomopathogenic microorganism that has demonstrated marked pathogenicity toward the larvae of a number of mosquito species, with little or no effect on nontarget insects. Therefore, the purpose of this study was to determine sublethal effects of L. chapmanii on fecundity, number of gonotrophic cycles, fertility and relationship between wing length and fecundity in Ae. aegypti females. Significant differences were observed (F= 210.38, df= 599, P&lt;0.0001; F= 164.11, df= 505, P&lt;0.0001; F= 95.48, df= 421, P&lt;0.0001; F= 74.94, df= 336, P&lt;0.0001; F= 81.08, df= 238, P&lt;0.0001; F= 47.54, df= 171, P&lt;0.0001) in the fecundity of females of Ae. aegypti exposed to zoospores of L. chapmanii with respect to controls in the first, second, third, fourth, fifth and sixth gonotrophic cycles respectively, differences were also observed in the number of gonotrophic cycles of females of Ae. aegypti that survived L. chapmanii infection as compared to controls (six cycles vs eight cycles) respectively. Significant differences (F= 110.90, df= 599, P&lt;0.0001) were also observed in the maximum length of the wing (1.96 mm + 0.59 in Ae. aegypti females that had been in contact with L. chapmanii zoospores vs 2.32 + 0.18 mm in controls). Significant differences were observed (F= 14.43, df= 5, P&lt;0.0191; F= 56.22, df= 5, P&lt;0.0017; F= 55.27, df= 5, P&lt;0.0017; F= 65.75, df= 5, P&lt;0.0013; F= 68.89, df= 5, P&lt;0.0012) in the fertility between females of Ae. aegypti exposed to zoospores of L. chapmanii with respect to controls in the second, third, fourth, fifth and sixth gonotrophic cycles respectively. &nbsp;&nbsp;This is the first study on the sublethal effects experienced by specimens of Ae. aegypti that survived infection with zoospores of L. chapmanii. Although field studies should be carried out, the results obtained in this study are encouraging because the high and rapid larval mortality caused by L. chapmanii coupled with the reduction of reproductive capacity in Ae. aegypti females seem to determine a significant reduction in the number of adults in the mid and long term, with the consequent reduction in the health risk involved. ","palabras_clave":"BIOCONTROL|DENGUE|MOSQUITOES|ZOOSPORES|FECUNDITY|GONOTROPHIC|CYCLES|FERTILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62925,"idioma_id":4,"titulo":"Survival and fecundity of Dichroplus maculipennis and Ronderosia bergi (Orthoptera: Acrididae: Melanoplinae) following infection by Beauveria bassiana (Ascomycota: Hypocreales) under laboratory conditions","resumen":"This study examined the effects of strain Beauveria bassiana (LPSC 1067) on nymphal development time, fecundity, and adult survival in Dichroplus maculipennis and Ronderosia bergi under laboratory conditions. It was observed that infection with 1x103 conidia\/ml altered nymphal development time, fecundity, and adult survival in both species. Mortality of D. maculipennis during third through the last instar (sixth) was significantly higher among treated nymphs (66 + 3.8 %) than in controls (15 + 1.7 %). Similarly, mortality in R. bergi during third through the last instar (fifth) was higher in treated nymphs (71 + 2.8 %) than in controls (19 + 1.5 %). Nymphal development times of both infected D. maculipennis and R. bergi were longer than controls. On the other hand, among survivors of both species, control adults lived longer than infected adults. Finally, control grasshoppers of both species were much more successful reproductively than infected grasshoppers.","palabras_clave":"DICHROPLUS MACULIPENNIS; |RONDEROSIA BERGI|GRASSHOPPERS|BEAUVERIA BASSIANA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62926,"idioma_id":4,"titulo":"About the linearity of the color-magnitude relation of early-type galaxies in the Virgo cluster","resumen":"We revisit the color-magnitude relation of Virgo Cluster early-type  galaxies in order to explore its alleged nonlinearity. To this aim, we  reanalyze the relation already published from data obtained within the  ACS Virgo Cluster Survey of the Hubble Space Telescope and perform our  own photometry and analysis of the images of 100 early-type galaxies  observed as part of this survey. In addition, we compare our results  with those reported in the literature from data of the Sloan Digital Sky  Survey. We have found that when the brightest galaxies and untypical  systems are excluded from the sample, a linear relation arises in  agreement with what is observed in other groups and clusters. The  central regions of the brightest galaxies also follow this relation. In  addition, we notice that Virgo contains at least four compact elliptical  galaxies besides the well-known object VCC 1297 (NGC 4486B). Their  locations in the lang&#956;effrang-luminosity diagram define a  trend different from that followed by normal early-type dwarf galaxies,  setting an upper limit in effective surface brightness and a lower limit  in the effective radius for their luminosities. Based on the  distribution of different galaxy sub-samples in the color-magnitude and  lang&#956;effrang-luminosity diagrams, we draw some conclusions  on their formation and the history of their evolution.","palabras_clave":"GALAXIES|CLUSTER|VIRGO|DWARF|ELLIPTICAL|LENTICULAR|PHOTOMETRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62927,"idioma_id":4,"titulo":"Escherichia coli beta-Galactosidase Inhibitors through Modifications at the Aglyconic Moiety: Experimental Evidence of Conformational Distortion in the Molecular Recognition Process","resumen":"Herein, we describe the use of thioglycosides as glycosidase inhibitors by employing novel modifications at the reducing end of these glycomimetics.  The inhibitors display a basic galactopyranosyl unit (1-4)-bonded to  a 3-deoxy-4-thiopentopyranose moiety. The molecular basis of the observed inhibition has been studied by using a combination of NMR spectroscopy and  molecular modeling techniques. It is demonstrated that these molecules are  not recognized by Escherichia coli bgalactosidase in their ground-state conformation, with a conformational selection process taking place. In fact, the  observed conformational distortion depends on the chemical nature of the  compounds and results from the rotation around the glycosidic linkage (variation  of F or Y) or from the deformation of the six-membered ring of the  pentopyranose. The bound conformations of the ligand are adapted in the  enzymatic pocket with a variety of hydrogen-bond, van der Waals, and stacking  interactions.","palabras_clave":"conformation analysis|enzyme models|glycosides|NMR spectroscopy|S ligands"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62929,"idioma_id":4,"titulo":"Muscular reconstruction and functional morphology of the forelimb of Santacrucian (Early Miocene) sloths (Xenarthra, Folivora) of Patagonia","resumen":"Early Miocene sloths are represented by a diversity of forms ranging from 38 to 95 kg, being registered mainly from Santacrucian Age deposits in southern-most shores of Patagonia, Argentina. Their postcranial skeleton differs markedly in shape from those of their closest living relatives (arboreal forms of less than 10 kg), Bradypus and Choloepus. In order to gain insight on functional properties of the Santacrucian sloths forelimb, musculature was reconstructed and a comparative, qualitative morphofunctional analysis was performed, allowing proposing hypotheses about biological role of the limb in substrate preferences, and locomotor strategies. The anatomy of the forelimb of Santacrucian sloths resembles more closely extant anteaters such as Tamandua and Myrmecophaga, due to the robustness of the elements, development of features related to attachment of ligaments and muscles, and conservative, pentadactylous, and strong-clawed manus. The reconstructed forelimb musculature was very well developed and resembles that of extant Pilosa (especially anteaters), although retaining the basic muscular configuration of generalized mammals. This musculature allowed application of powerful forces, especially in adduction of the forelimb, flexion and extension of the antebrachium, and manual prehension. These functional properties are congruent with both climbing and digging activities, and provide support  for proposed Santacrucian sloths as good climbing mammals, possibly arboreal or semiarboreal, being also capable diggers. Their climbing strategies were limited, thus these forms relied mainly on great muscular strength and curved claws of the manus to move cautiously on branches.","palabras_clave":"FOLIVORA|EARLY MIOCENE|FUNCTIONAL MORPHOLOGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62930,"idioma_id":4,"titulo":"Factors  capable of modifying the response of Pseudomonas aeruginosa   to the  inactivation induced by heterogeneous photocatalysis","resumen":"The heterogeneous photocatalysis (HP) procedure hasbeen demonstrated to be an interesting method for disinfecting water. It is effective for inactivating Pseudomonas aeruginosa, but its effectiveness may be reduced due to the action of several factors which are able to affect bacterial radio-sensitivity. The results reported here show the influence of nutritional stress and pre-exposure to sub-lethal UVA doses on the efficiency of the inactivation of P. aeruginosa by HP. Both previous exposures to low UVA fluencies and nutrient deprivation induce bacterial resistance to this process, in concordance with previous observations about the lethal effect of direct UVA irradiation. Starvation plus pre-irradiation did not have synergistic or antagonist effects. Kinetic parameters are presented for all three cases. These factors should be taken into account in the design of a water treatment process.   ","palabras_clave":"PSEUDOMONAS|PHOTOCATALYSIS|TiO2|SUNLIGHT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62931,"idioma_id":4,"titulo":"Reconstruction of palaeoprecipitation based on pollen transfer functions \u2013 the record of the last 16 ka from Laguna Potrok Aike, southern Patagonia","resumen":"Based on modern pollen samples from different vegetation units in southern Patagonia, showing a close relation to yearly amounts of precipitation and mean annual temperatures, different polleneclimate  transfer functions are developed and tested. Comparing the performance of MAT (Modern Analogue Techniques), WA (Weighted Average), as well as WAPLS (Weighted Average Partial Least Square) statistical techniques, it is possible to determine the statistically most robust model (WAPLS for precipitation). This transfer function is then used to estimate palaeoprecipitation amounts based on Laguna Potrok Aike pollen results for the last 16,000 years. Generally, the results of the precipitation model indicate less precipitation during the Lateglacial and alternating wet and dry periods during the Holocene. The Holocene started with higher amounts of precipitation until about 8 ka cal. BP, followed by a period with lower amounts between 8 and 2.5 ka cal. BP, while the Late Holocene shows a general increase in precipitation. Comparisons with former shoreline reconstructions and carbonate concentrations in the sediments of Laguna Potrok Aike not always show similarities due to the complex environmental factors recorded by these proxies. Moreover, changes in the moisture availability due to the interplay of precipitation and  Temperature, cannot be reconstructed directly. Nevertheless, the general  long-term trend of palaeoprecipitation is in accordance with the absolute moisture content in the air, which is determined mainly by temperature: during cold periods with less absolute moisture, the model  shows less precipitation. Moreover, the model also points to a relation with the position and strength of the Southern Hemisphere Westerlies.","palabras_clave":"ICDP-PROJECT PASADO|MODERN POLLEN SAMPLES|TRANSFER FUNCTIONS|POLLEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62932,"idioma_id":4,"titulo":"The growth rate modulates time to first bud appearance in Physaria mendocina","resumen":"Physaria mendocina is under domestication because its seeds contain significant amounts of hydroxy fatty acids for several industrial uses, but displays a facultative biennial behavior which may represent a drawback in terms of production. Previous work revealed that the time to flower induction in this species is insensitive to temperature, photoperiod and vernalization, but suggested that this length of time could be determined by radiation, water and nutrients and\/or the acquisition of a minimum growth rate. We aimed to determine whether the attainment of a threshold plant growth rate (GRt) triggers the initiation of the flowering phase in P. mendocina. Nutrient, water and radiation availability were manipulated to modify the timing of acquisition of that rate, expecting a concomitant modification of the time to flowering. We also explored the possibility that the stimulus is mediated by an accumulation of active gibberellins (GAs). Linear regressions were fitted between plant dry weight and time, and slopes of the relationships were considered as the growth rates. Radiation, water and nutrients constraints increased the duration of the phase between emergence and, concomitantly, first bud appearance (FBA). However, plants from all treatments reached FBA, after acquiring a growth rate of around 0.01 g d&#8722;1 &#8722;pl &#8722;1 (GRt).When exogenous GAs was applied under limiting radiation, plants reached FBA despite the fact that they never acquired a GRt; conversely, when GAs biosynthesis was inhibited under high irradiances, the plants required more days to reach FBA than controls, despite the fact that they acquired a GRt. The information obtained allow us to conclude that the time to FBA, which is the first visible manifestation of floral induction in this system, is modulated by factors controlling growth mediated by an accumulation of (GAs) and suggest that the acquisition of GRt is the internal feature that triggers floral induction. This knowledge offers a frame within which cropping systems could be designed in order to avoid or not a biennial behavior.","palabras_clave":"Physaria mendocina|Development  |Floral induction|Radiation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62933,"idioma_id":4,"titulo":"The Potential Role of Epigenetics on Multipotent Cell Differentiation Capacity of Mesenchymal Stromal Cells","resumen":"Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts.","palabras_clave":"MESENCHYMAL STROMAL CELLS|TELOMERASE|PLURIPOTENCY FACTORS|EPIGENETICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62934,"idioma_id":4,"titulo":"Neuronal ceroid lipofuscinosis type CLN2: A new rationale for the construction of phenotypic subgroups based on a survey of 25 cases in South America","resumen":"a b s t r a c tTripeptidyl-peptidase 1 (TPP1) null or residual activity occurs in neuronal ceroid lipofuscinosis (NCL) with underlyingTPP1\/CLN2 mutations. A survey of 25 South American CLN2 affected individuals enabled the differentiationof two phenotypes: classical late-infantile and variant juvenile, each in approximately 50% of patients, withresidual TPP1 activity occurring in approximately 32%. Each individual was assigned to one of three subgroups:(I) n=11, null TPP1 activity in leukocytes; (II) n=8, residual TPP1 activity of 0.60?15.85 nmol\/h\/mg (nr110?476); (III) n=6, activity notmeasured in leukocytes. Curvilinear bodies (CB) appeared in almost all studiedCLN2 subjects; the only exceptions occurred in cases of subgroup II: two individuals had combined CBs\/fingerprints(FPs), and one case had pure FPs. There were 15 mutations (4 first published in this paper, 3 previouslyobserved in South America by our group, and 8 previously observed by others). In subgroup I, mutations wereeither missense or nonsense; in subgroups II and III, mutations prevailed at the non-conserved intronic site,c.887&#8722;10A&gt;G (intron 7), and to a lesser extent at c.89+5G&gt;C (intron 2), in heterozygous combinations.Grouping phenotypically and genetically known individuals on the basis of TPP1 activity supported the conceptthat residual enzyme activity underlies a protracted disease course. The prevalence of intronic mutations atnon-conserved sites in subgroup II individuals indicates that somealternative splicingmight allowsome residualTPP1 activity.© 2013 Elsevier B.V. All rights reserved.","palabras_clave":"Neuronal ceroid lipofuscinoses|Tripeptidyl-peptidase 1|TPP1\/CLN2 gene|Phenotype"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62935,"idioma_id":4,"titulo":"Ashes in the air: the effects of volcanic ash emissions on plant--pollinator relationships and possible consequences for apiculture","resumen":"Pollinator foraging performance could be altered by volcanic ash   contaminated flowers, pollen, and nectar. We used the honeybee (Apis mellifera) as a model organism to understand the effects that volcanic ash could have on apiculture and establish some of the mechanisms through which it could affect plant-pollinator interactions. Three mechanisms were investigated: (1) interference with resource location, (2) interference with resource consumption, and (3) disturbing digestive processes. Results indicate that plant-pollinator relationships could be altered by volcanic ash. On the one hand, honeybees seem to recognize flowers covered in ashes only after an adaptation period (i.e., learning). On the other hand, there is no avoidance mechanism to prevent ingestion of contaminated food that ultimately reduces survival. Apiculture could be negatively affected due to this natural disturbance and plant-pollinating relationships could be especially vulnerable to ash   emissions due to the high exposure of pollen and nectar bearing structures susceptible to contamination. Additionally, nectar feeders gut morphology (i.e., convoluted, thin with no resistance to abrasion) enables ash particles in contaminated food to obstruct and lacerate the gut increasing mortality risk.","palabras_clave":"Apis mellifera|disturbance|pollinator|volcanic ash - volcanic complex Puyehue Cordon Caulle"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62936,"idioma_id":4,"titulo":". Development of solar arrays forargentine satellite missions","resumen":"This paper presents the development of solar arrays for Argentine satellite missions performed withinthe frame of cooperation agreements between the Argentine National Atomic Energy Commission (CNEA)and the Argentine National Commission for Space Activities (CONAE). Relevant issues such as simulationand design, solar cells characterisation, set up of soldering and bonding processes, radiation damage anddevelopment of testing techniques, are reviewed. A chamber for testing solar cells and other devices in asimulated space environment, and the integration and tests of two engineering models for qualificationof space technology processes are described. The requirements and solar array design for 3 missions,Aquarius\/SAC-D and SAOCOM 1A and 1B, are presented. Finally, the flight model for the SAC-D mission,which was launched on 10 June 2011, is outlined, and telemetry data analysis from the first stages of themission is presented.","palabras_clave":"PV Array|Space Cells|Radiation Damage|Enviromental Test"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62937,"idioma_id":4,"titulo":"A theoretical approach to photosynthetically active radiation silicon sensor","resumen":"Artículo enviado para su evaluación:&nbsp; This paper presents a theoretical approach for the development of low cost radiometers to measure photosynthetically active radiation (PAR). Two alternatives are considered: a) glass optical filters attached to a silicon sensor, and b) dielectric coating on a silicon sensor. The devices proposed are based on radiometers previously developed by the Argentine NationalAtomic Energy Commission (CNEA). The objective of this work is to adapt these low cost radiometers to construct low cost but reliable instruments for measuring PAR.The transmittance of optical filters and sensors response have been analyzed for different dielectricmaterials, number of layers deposited, and incidence angles. Uncertainties in thickness of layerdeposition were evaluated.","palabras_clave":"photosinthetically active radiation|radiometers|sun sensors|silicon devices"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62938,"idioma_id":4,"titulo":"Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform","resumen":"In this article,we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode(SPCE)applied to determination of clenbuterol(CLB)in bovine hair samples.CLB is member of the b2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning.  In order to perform the CLB detection,the SPCE was modified by gold nanoparticles(AuNPs)electrodeposition.Quantitative determination of CLB was carried out using a competitive indirect immunoassay,method based on the use of anti-CLB antibodies immobilized on magnetic micro  particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase(AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl  phosphate was converted to p-aminophenol by AP,and the electroactive product was quantified on AuNPs\/SPCE at þ 0.1 V. The limit of detection for electrochemical method was 0.008 ngmL1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an ??in vitro?? technique.","palabras_clave":"Microfluidic immunosensor|Clenbuterol|Bovine hair samples|Gold nanoparticles|Magnetic micro particles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62939,"idioma_id":4,"titulo":"Distinguishing similar volcanic source areas from an integrated provenance analysis: Implications for foreland andean basins","resumen":"The Mata Amarilla Formation marks the onset of the foreland stage of the Austral basin, which is composed of mostly nonmarine and littoral siliciclastic sediments, thus providing an opportunity to study the detrital record of the Late Cretaceous southern foreland Andes. Our dataset provides, for the first time, a comprehensive picture of the Late Cretaceous evolution of the Austral foreland basin, constituting a possible analogue to other foreland basins at the foot of the Andes.  Sandstones from the Mata Amarilla Formation testify to variable contributions from Jurassic bimodal volcanic rocks of the Deseado Massif and the Patagonian fold-and-thrust belt, in the context of an eastward-advancing orogen. Sandstone petrography shows an overall feldspathic litharenite composition, whereas sandstones coming from the northeast (Deseado Massif) have higher Lv and lower Qp proportions than samples coming from the west (Patagonian fold-and-thrust belt). In the  central part of the study area, sandstones are characterized by higher proportions of Qt associated with a greater distance and time of transport relating to its position in the Austral foreland basin. In spite of the increased maturity of sandstone in the central area, X-ray analyses permit recognition of the compositional signature of Mata Amarilla Formation, in which four claymineral assemblages were identified: S (rich in smectite), S-K (rich in smectite and kaolinite), Pg (rich in palygorskite), and I\/S (rich in illite\u2013smectite mixed layers). S-assemblage evidences well-crystallized smectite, characteristic of volcaniclastic origin.  Most smectite was formed during early diagenesis through alteration of labile tuffaceous material derived from the Southern Andes. The stratigraphic variations in clay-mineral assemblages reveal a strong environmental control on their distribution. The transformation of smectite into illite and kaolinite is considered as product of pedogenesis, whereas the presence of palygorskite indicates a coastal environment with paleosol development under poorly drained conditions.","palabras_clave":"Upper Cretaceous|greenhouse|Mata Amarilla Formation|Austral Foreland Basin|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62940,"idioma_id":4,"titulo":"Study of the water absorption and its influence on the Young's modulus in a commercial polyamide","resumen":"The water absorption and desorption processes of a commercial polyamide were studied in the temperature range of 20-90 °C. A strong influence of the temperature on the water mass saturation, primarily in the region of the glass transition temperature, was observed. Differences in the parameters related to the diffusion process were also observed between samples with different thicknesses. The results were analyzed using the Langmuir model, which considers two states of absorbed water in the polymer: bound and mobile. The obtained diffusion coefficients were analyzed in terms of the temperature and the state of the polymer, either glassy or rubbery. The influence of the absorbed water on the Young's modulus was evaluated using the impulse excitation technique. It was observed that the water absorbed in the bound state generates the most significant changes in the Young's modulus.","palabras_clave":"WATER ABSORPTION|POLYAMIDE|YOUNGS MODULUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62941,"idioma_id":4,"titulo":"Fractional statistical theory of adsorption applied to protein adsorption","resumen":"Experimental adsorption isotherms of bovine serum albumin (BSA) adsorbed on sulfonated microspheres were described by means of two analytical models: the first is the well-known Langmuir?Freundlich model (LF), and the second, called fractional statistical theory of adsorption (FSTA), is a statistical thermodynamics model developed recently by Ramirez-Pastor et al. [Phys. Rev. Lett. 93 (2004) 18610193 (2004) 186101]. The experimental data, obtained by Hu et al. [Biochem. Eng. J. 23 (2005) 259] for different concentrations of sulfonate group on the surface of the microspheres, were correlated by using a fitting algorithm based on least-squares statistics. The combination of LF and FSTA models, along with the choice of an adequate fitting procedure, allowed us to obtain several conclusions: (i) as previously reported in the literature, the maximum amount adsorbed increases as the amount of sulfonate group increases; (ii) the equilibrium constant does not appear as a sensitive parameter to the amount of sulfonate group on the surface of the microspheres; and (iii) the values of the fitting parameters obtained from FSTA may be indicative of a mismatch between the equilibrium separation of the intermolecular interaction and the distance between the adsorption sites. The exhaustive study presented here has shown that FSTA model is a good one considering the complexity of the physical situation, which is intended to be described and could be more useful in interpreting experimental data of adsorption of molecules with different sizes and shapes.CallSend SMSCall from mobileAdd to SkypeYou'll need Skype CreditFree via Skype","palabras_clave":"Adsorcion|BSA|Constante de Adsorcion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62942,"idioma_id":4,"titulo":"Impact of structural and textural membrane properties on lemon juice clarification","resumen":"Lemon juice was clarified using membranes with different structural and textural properties. Membranes were prepared from 0, 5, 7 and 10 wt.% of PVP in PVDF and they were structurally and functionally characterized. Results indicated that the addition of PVP produced both structural and surface textural changes in the membranes. These textural changes resulted in an increase of apparent hydrophobicity in the membranes prepared from 5 and 7% of PVP in the casting solution. Besides, the presence of residual PVP in the membrane favors hesperidin adsorption enhancingits retention. Analysis of the clarified juice indicated that the membrane prepared with 5% of PVP possessed the highest efficiency, combining high permeate flux and high juice quality.","palabras_clave":"Lemon Juice|Clarification|Membrane|PVDF"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62943,"idioma_id":4,"titulo":"Miscibility in Crystalline\/Amorphous Blends of Poly(3-hydroxybutyrate)\/ DGEBA","resumen":"A differential scanning calorimetry (DSC) and smallangle X-ray scattering (SAXS) study of miscibility in blends of the semicrystalline polyester poly(3-hydroxybutyrate) (PHB) andamorphous monomer epoxy DGEBA (diglycidyl ether of bisphenol A) was performed. Evidence of the miscibility of PHB\/DGEBA in the molten state was found from a DSC study of the dependence of glass transition temperature (Tg) as a function of the blend composition and isothermal crystallization, analyzing the meltin g point (Tm) as a function of blend composition. A negative value of Flory?Huggins interaction parameter vPD was obtained. Furthermore, the lamellar crystallinity in the blend was studied by SAXS as a function of the PHB content. Evidence of the segregation of the amorphous material out of the lamellar structure was obtained","palabras_clave":"BIODEGRADABLE|BLENDS|MISCIBILITY|CRISTALLIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62944,"idioma_id":5,"titulo":"Separación de fases y cristalinidad en mezclas epoxy\/poli-3-hidroxibutirato","resumen":"In this work, epoxy\/poly-3-hydroxybutyrate (PHB) blends in two different proportions, 90\/10 and 85\/15,  were studied. For this purpose, the samples were submitted to 80ºC and 120ºC for the curing process of epoxy. The  phase separation and the crystallization were analyzed using optical microscopy and differential scanning calorimetry.  Likewise, the lamellar structure of PHB was characterized using small angle X-ray scattering. Significant differences  between the two proportions studied were found; while the 90\/10 sample presents miscibility, the 85\/15 sample indicates  a phase separation. Besides, the lamellar structure of PHB is affected due to the epoxy, mainly in the 90\/10 sample. The  results were analyzed on the basis of the competition between crystallization and phase separation processes.","palabras_clave":"MEZCLAS POLIMÉRICAS|EPOXY|PHB|CRISTALIZACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62945,"idioma_id":5,"titulo":"¿Cuántos son los pobres? Contribuciones a la historia de su definición estadística en la Argentina de los años ochenta","resumen":"En 1984, el Instituto  Nacional de Estadísticas y Censos (INDEC) publica un informe titulado La pobreza en Argentina. A partir de los  datos del Censo nacional de población de 1980, se buscaba elaborar mapas de  necesidades básicas insatisfechas para todo el territorio nacional. Este  informe recorre y analiza la situación social del país luego de siete años de  dictadura, con el objeto de contribuir a la orientación de los recursos  distribuidos por las políticas sociales, en especial por el entonces flamante  Programa Alimentario Nacional (PAN). Por primera vez, el Estado produce un  estudio sistemático de los problemas sociales del país en términos de pobreza,  corriendo el foco de la cuestión del trabajo, sobre la que las estadísticas  sociales se habían ocupado de manera dominante. Esta categoría, que hoy aparece  como un dato de la realidad, es el resultado de un largo y complejo trabajo de  construcción técnica, moral y política del que nos ocupamos en este artículo.","palabras_clave":"POBREZA|ESTADÍSTICAS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62946,"idioma_id":4,"titulo":"Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions","resumen":"Nitrogen is the critical factor for crop production. Currently, commercial inoculants arebased on the selection of Rhizobiales isolates capable of fixing nitrogen efficiently insymbiosis with legumes. In this study, the beneficial rhizobacterium Pseudomonasprotegens Pf-5 was genetically modified to fix nitrogen using the genes encoding thenitrogenase complex of Pseudomonas stutzeri A1501 via the X940 cosmid. Therecombinant strain Pf-5 X940 was able to grow in L medium without ammoniumsulphate, displayed high nitrogenase activity and released significant quantities ofammonium into the medium. Interestingly, the expression of nif genes and thenitrogenase activity of Pf-5 X940 were not affected by ammonium, suggesting aconstitutive nitrogen fixation in this recombinant strain. Furthermore, inoculation ofArabidopsis, Alfalfa and Tall fescue with Pf-5 X940 increased ammonium availabilityand plant productivity under nitrogen-deficient conditions. Similar to Pseudomonasprotegens Pf-5, Pseudomonas putida KT2440, Pseudomonas veronii DSM11331 andPseudomonas taetrolens IAM1653 but not Pseudomonas balearica SP1402 andPseudomonas stutzeri CCUG11256 transformed with X940 cosmid showedconstitutive nitrogenase activity suggesting that nitrogenase regulation depend on thegenome context. Taken together, these results open the way to the production ofeffective recombinant inoculants for nitrogen fixation on a wide range of crops.","palabras_clave":"Nitrogen Fixation |Pseudomonas protegens |horizontal transfer|crop productivity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62947,"idioma_id":5,"titulo":"ACUMULACIÓN DE ACETALDEHÍDO Y ESTRÉS OXIDATIVO EN TESTÍCULO LUEGO DE LA INTOXICACIÓN ALCOHÓLICA EN RATAS","resumen":"En el hombre el consumo excesivo de alcohol está asociado con una disminuciónen la producción de testosterona y la atrofia testicular. Pudieron observarseconsecuencias similares en estudios realizados in vitro en testículosaislados y la producción de testosterona, donde el acetaldehído mostró sermás potente que el alcohol para la supresión de la liberación de la hormona.Estudios previos de este laboratorio reportaron que la fracción microsomal deltestículo de rata era capaz de metabolizar el etanol a metabolitos reactivoscomo el acetaldehído y los radicales libres 1-hidroxietilo. En este trabajo sepresenta evidencia de que luego de una dosis única de etanol, el acetaldehídose acumula en el testículo durante las primeras seis horas posteriores al tratamientopara alcanzar concentraciones superiores a las plasmáticas aunquemás bajas que en el hígado. Además se encontró que la actividad aldehídodeshidrogenasa presente en el testículo es significativamente menor que enel hígado. El consumo de etanol en los animales produjo una susceptibilidadaumentada a la oxidación de los lípidos testiculares, que fue detectada porniveles aumentados de hidroperóxidos de lípidos. Los resultados sugieren quela oxidación in situ del etanol a acetaldehído y a radicales libres y la detoxificacióndeficiente podrían ser relevantes para explicar los efectos observados.","palabras_clave":"CONSUMO DE ALCOHOL |TOXICIDAD REPRODUCTIVA |TESTICULO|ESTRES OXIDATIVO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62948,"idioma_id":4,"titulo":"Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery","resumen":"Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed bypolyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was foundto be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5.Partition coefficients values and low entropic and enthalpic changes associated with tannase partitionsuggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottomphase due to the high molar mass of the enzyme. The process was significantly influenced by thetop phase\/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems,a good performance was found, since the enzyme recovery in the bottom phase of the system composedby polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity","palabras_clave":"tannasse|aquous two phases|bioseparation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62949,"idioma_id":4,"titulo":"Presence of amphibole in vermiculite mined in Argentina. Morphology, quantitative and chemical studies on the different phases of production","resumen":"Vermiculites with impurities of amphibole, adry fine residue and calcined materials from three depositsin the province of Co´rdoba, Argentina, were studied. Froma commercial perspective, amphiboles are considered as??asbestos??, a group of silicate minerals with strong andflexible fibres that are heat resistant and chemically inert,and thus well suited for heat insulation. These fibrousparticles have provoked controversies about the toxicityimpact on human health and the development of diseasessuch as mesothelioma, asbestosis or lung cancer. Theircommercialization and exploitation are currently prohibited.The sampled minerals were identified with a petrographicmicroscope, chemical analyses, SEM and XRD. Itwas concluded that amphibole minerals are present in allthe deposits studied and in every phase of plant production,but not all of them have asbestiform characteristics.","palabras_clave":"Vermiculite|Amphibole|Asbestos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62950,"idioma_id":4,"titulo":"Sensitive ergotamine determination in pharmaceuticals and biological samples using cloud point preconcentration and spectrofluorimetric detection","resumen":"A new cloud point extraction (CPE) method for ergotamine analysis using fluorimetric detection is described. Ergotamine from an aqueous solution was preconcentrated into a smaller surfactant-rich phase using nonionic surfactant polyoxyethylene(7.5)nonylphenylether (PONPE 7.5). Differently from the conventional CPE procedure in which the resulting surfactant-rich phase is diluted by a fluidificant before its analysis, in this method the fluorescence measurements were carried out directly onto the undiluted surfactant-rich phase. The high viscosity provided by the undiluted surfactant rich phase greatly improved the fluorescence emission of ergotamine, leading to a total enhancement factor of 1325. This spectral advantage plus the preconcentration factor achieved, contributed to the method sensitivity allowing the ergotamine determination at trace level concentration. Under optimal experimental conditions, a linear calibration curve was obtained from 3.81×10&#8722;7 to 1.10 g mL&#8722;1, with detection and  quantification limits of 0.11 and 0.38 pg mL&#8722;1, respectively. The accuracy and versatility of the present methodology were proved by analyzing ergotamine in real samples of different natures such as pharmaceuticals,  urine and saliva.","palabras_clave":"Ergotamine |Cloud point extraction |Spectrofluorimetry |Pharmaceuticals |Urine|Saliva"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62951,"idioma_id":4,"titulo":"Solid fat content determination by differential scanning calorimetry: Prior treatment and proposed correction.","resumen":"The objective of this work was to develop acorrected method for solid fat content estimation by dif-ferential scanning calorimetry (DSC), as important differ-ences are usually observed between the results given byDSC and pulsed nuclear magnetic resonance (NMR). Coldstorage after full melting of fats was necessary to avoid theappearance of exothermic peaks in the modulated tem-perature DSC thermograms, in order to make an appro-priate estimation of melting energy. Different fats wereanalyzed by NMR and DSC, obtaining considerably highersolid fat content values with the latter, uncorrected method.These differences were attributed to the fact that consumedenergy per unit of melted mass tends to increase with theincrease of the melting temperature of each fraction of thefats. A linear correlation between melting enthalpy andmelting point of different triglycerides was used to estimatethe energy per unit of mass consumed at each temperature.From these data, an estimated transformation of meltingenergy into melted mass was performed and new solid fatcontent values were calculated. The results obtained fromthis correction were much closer to the measurementsmade by NMR, in comparison to the uncorrected DSCmethod.","palabras_clave":"DIFFERENTIAL SCANNING CALORIMETRY|PULSED NUCLEAR MAGNETIC RESONANCE|SOLID FAT CONTENT|MELTING ENTHALPY|COMPARISON OF TECHNIQUES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62952,"idioma_id":5,"titulo":"Disputas y tensiones en la construcción de la verdad jurídica: las distintas versiones construidas alrededor de tres casos de violencia policial","resumen":"A partir del trabajo con una fuente judicial -el expediente-, en este artículo nos proponemos analizar diferentes lógicas, saberes y dinámicas burocráticas que van construyendo y expresando distintas versiones en un caso judicial. Particularmente, nos interesa acceder a las lógicas de construcción de la verdad jurídica y a las disputas que se dieron en torno a ella entre diferentes agentes estatales y no estatales -policías, funcionarios judiciales, abogados y víctimasa partir de tres casos de utilización letal de la fuerza por parte de funcionarios de la Policía provincial de Santa Fe, concretamente en la ciudad de Rosario. Este análisis permitirá señalar cómo, obedeciendo al marco de relaciones en que se encuentran y posicionan determinados agentes, las resoluciones judiciales pueden tomar caminos diferentes que el delineado inicialmente por la versión policia","palabras_clave":"VERSIONES|VERDAD JURIDICA|VIOLENCIA POLICIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62953,"idioma_id":5,"titulo":"Vulnerabilidad y Riesgo en Asunción, Paraguay. Re-enfocando la discusión mediante la aplicación de un dispositivo para pensar la vulnerabilidad social y el riesgo de la vivienda para la salud","resumen":"Resultados de la aplicación de la metodología MDRS y el software RVS en Asunción del Paraguay.","palabras_clave":"VULNERABILIDAD_SOCIAL|RIESGO|VIVIENDA|SALUD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62954,"idioma_id":4,"titulo":"Scleroglucan compatibility with thickeners, alcohols and polyalcohols and downstream processing implications","resumen":"Thickening capacity and compatibility of scleroglucan with commercial thickeners (corn starch, gum arabic, carboxymethylcellulose, gelatin, xanthan and pectin), glycols (ethylene glycol and polyethylene glycol), alcohols (methanol, ethanol, 1-propanol and isopropanol) and polyalcohols (sorbitol, xylitol and mannitol) was explored. Exopolysaccharides (EPSs)from Sclerotium rolfsiiATCC 201126 and a commercial scleroglucan were compared. Compatibility and synergism were evaluated taking into account rheology, pH and sensory properties of different thickener\/scleroglucan mixtures in comparison with pure solutions. S. rolfsii ATCC 201126 EPSs induced or increased pseudoplastic behaviour with a better performance than commercial scleroglucan, showing compatibility and synergy particularly with corn starch, xanthan, pectin and carboxymethylcellulose. Compatibility and a slight synergistic behaviour were also observed with 30% (w\/v) ethylene glycol whereas mixtures with polyethylene glycol (PEG) precipitated. Scleroglucan was compatible with polyalcohols, whilstlower alcohols led to scleroglucan precipitation at 20% (v\/v) and above. PEG-based scleroglucan downstream processing was compared to the usual alcohol precipitation. Downstream processed EPSi (with isopropanol) and EPS-p (with PEG) were evaluated on their yield, purity, rheological properties and visual aspect pointing to alcohol downstream processing as the best methodology, whilst PEG recovery would be unsuitable. The highest purified EPSi attained a recovery yield of &#8764;23%, similar to ethanol purification, with a high degree of purity (88%, w\/w vs. EPS-p, 8%, w\/w) and exhibited optimal rheological properties, water solubility and appearance. With a narrower molecular weight distribution (Mw, 2.66 × 106 g\/mol) and a radius of gyration (Rw, 245 nm) slightly lower than ethanol-purified EPSs, isopropanol downstream processing showed to be a proper methodology for obtaining a refined-grade scleroglucan.","palabras_clave":"Scleroglucan compatibility |compatibility|alcohols and polyalcohols |downstream"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62955,"idioma_id":4,"titulo":"Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior","resumen":"Aqueous solutions (0.2%, w\/v) of scleroglucans from Sclerotium rolfsii ATCC 201126 from different cultivation time or purification protocol (EPS I, EPS II, EPSi) as well as a commercial scleroglucan (LSCL) exhibited different sensitivity against thermal (65, 95 and 150 &#9702;C), ultrasonic (1, 5 and 10 min; 20% amplitude) or alkaline (0.01?0.2 N NaOH) treatments. Scleroglucan triple helix usually showed signs of denaturation at 150 &#9702;C or with 0.2 NaOH with a pronounced decrease in apparent viscosity and loss of pseudoplastic behavior. Differences in sensitivity could be noted depending on the scleroglucan sample, which may be likely related to polysaccharide conformational features, and these latter to production and\/or downstream processing conditions. Transmission electronmicroscopy showed scleroglucan topologies in accordance with thermal and alkaline denaturation. Size exclusion chromatography of control scleroglucans revealed elution profiles compatible with macromolecular aggregates which tended to diminish or disappear as thermal, alkali or sonication treatments progressed. Scleroglucan granule dissolution process took &#8764;8?14 s, according to DIC light microscopy, and showed to be facilitated by addition of NaOH.","palabras_clave":"Scleroglucan|Thermal  treatments|Alkaline  treatments|Ultrasonic  treatments"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62956,"idioma_id":4,"titulo":"Relationship among carotenoid production, copper bioremediation and oxidative stress in Rhodotorula mucilaginosa RCL-11","resumen":"Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a   filter plant of a copper mine in the province of Tucumán, Argentina,   supports high concentrations of the heavy metal Cu(II). Copper overload   augmented carotenoid biosynthesis in this yeast, modifying at the same   time the relative proportion of the pigments produced. Inhibition of the   synthesis pathway with diphenylamine suggests an inverse relationship   between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The   increased activity of superoxide dismutase and catalase measured under   inhibition of carotenoid biosynthesis could explain these observations.   Exposure to H2O2, a second oxidative stress agent,   alone or in combination with Cu(II) also modified the carotenoid   content, both qualitatively and quantitatively. The change in the   relative proportion of the carotenoids torularhodin, torulene and   beta-carotena, as well as the detection of gamma-carotene in the   presence of H2O2 and Cu(II) allows to hypothesize   that the carotenoids produced by R. mucilaginosa RCL-11 plays different   roles in the oxidative stress response of this yeast..","palabras_clave":"CAROTENOID|RHODOTORULA MUCILAGINOSA|COPPER|BIOREMEDIATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62957,"idioma_id":5,"titulo":"PUEBLOS INDÍGENAS URBANOS EN ARGENTINA: UN ESTADO DE LA CUESTIÓN","resumen":"La problemática de los pueblos indígenas urbanos, resulta un particular desafío para la reflexióndesde las ciencias sociales y la disciplina antropológica. Máxime considerando, que en Argentina la mayor parte de los pueblos indígenas reside en ámbitos urbanos y si bien se vienen incrementando recientemente los diferentes estudios en esta línea, ésta ha sido una temática escasamente desarrollada. Nos proponemos como objetivo efectuar una aproximación general de la cuestión indígena urbana contemporánea en Argentina, considerando la dinámica migratoria y los procesos organizativos en ámbitos urbanos. Ejemplificaremos esta problemática a partir de situaciones correspondientes a los tres grupos indígenas de mayor incidencia demográfica del país: el Pueblo Mapuche \u2013a partir del caso de pobladores que han migrado a diversas ciudades de Norpatagonia-, el pueblo Qom y el pueblo Guaraní. En estos últimos dos grupos, analizando los procesos de las comunidades \u201CNogoyin Ni Nala\u201D (Qom y Moqoit) y \u201CCacique Hipólito Yumbay\u201D respectivamente, ambas en el Partido de Almirante Brown, en el Gran Buenos Aires (Provincia de Buenos Aires). Nos planteamos como objetivo complementario, formular una serie de lineamientos para el abordaje de esta temática, que sirva como insumo para profundizar en aportes futuros.","palabras_clave":"Indígenas urbanos|Migraciones|Procesos organizativos|Pueblo Mapuche"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62958,"idioma_id":4,"titulo":"The spatial variation in ant species composition and functional groups across the Subantarctic-Patagonian transition zone","resumen":"The role of ecotones in the maintenance of species diversity is rather controversial; they may represent&nbsp;either biodiversity hotspots with unique and rare forms, or&nbsp;be transitional areas that hold marginal populations of&nbsp; species. We analyse the taxonomic and functional composition of ant species assemblages across the Subantartic-Patagonian transition to evaluate the role that transitional&nbsp;shrublands may play in the maintenance of the taxonomic&nbsp;and functional differentiation. We collected ants using 450&nbsp;pitfall traps within a ~150&nbsp;x 150 km area. Species were&nbsp;classified into functional groups in relation to stress and&nbsp;disturbance, and in foraging groups according to their&nbsp;foraging behavior. An indicator value for each species in&nbsp;each habitat was calculated. The steppes and the forests&nbsp;strongly differed in ant species and functional composition.&nbsp;Climatic effects combined with structural components of&nbsp;plant environment explained about 23\u201327 % of the variation in ant composition. The shrublands did not show a&nbsp;distinctive fauna, and show greater similarity in ant species&nbsp;composition and in the proportional occupancy of functional groups to the steppes than to the forests. They harbor&nbsp;neither rare nor indicator species, except for Lasiophanes&nbsp;valdiviensis, and thus this reinforces the idea that they are&nbsp;not a habitat source of species, but an area of encounter&nbsp;between two distinct forest- and steppe- ant faunas, where a high number of local distributional limits of ant species overlap.","palabras_clave":"Formicidae|Ecotone|Biogeographic transition|IndVal|RDA|Cluster analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62959,"idioma_id":4,"titulo":"Soybean protein films. Characterization and Potential as Novel Delivery Devices of Duddingtonia flagrans Chlamydospores","resumen":"This study was aimed at assessing the potential use of soybean protein concentrate (SPC) ? based film as  vehicle for the delivery of Duddingtonia flagrans chlamydospores for the biological control of gastric nematodes  in ruminals. Glycerol and dialdehyde starch (DAS) were used as plasticizer and cross-linking  agent, respectively. Films were obtained by casting and characterized in terms of their physico-mechanical  properties. The impact of cross-linking extent on moisture absorption, swelling and tensile properties  of the resultant films was evaluated. The adequate tensile properties and stability in wet environment of  SPC films cross-linked with 10 wt.% DAS was counterbalanced with the two-phase morphologies developed,  irrespective of glycerol content, limiting their potential application as delivery devices. SPC films  cross-linked with 5 wt.% DAS and plasticized with 30 wt.% glycerol exhibited the best compromise  between solubility (only 29%), homogeneous morphology, adequate tensile strength (2.50 ± 0.43 MPa)  and elongation at break (18.72 ± 2.34%) and swelling profile. The preliminary results of the release of  D. flagrans chlamydospores in ruminal fluid revealed a slow release profile attaining 4.9% in a period of  24 h. Overall, these results substantiated the potential use of DAS-cross-linked SPC films as viable carrier  matrix for D. flagrans release applications","palabras_clave":"SOYBEAN PROTEIN CONCENTRATE|BIOLOGICAL CONTROL |DISSOLUTION PROPERTIES|Duddingtonia. flagrans chlamydospores"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62960,"idioma_id":4,"titulo":"Altitudinal variation in the taxonomic composition of ground-dwelling beetle assemblages in NW Patagonia, Argentina: environmental correlates at regional and local scales","resumen":"Abstract. 1. Altitudinal gradients offer a unique scenario to elucidate how theincrease in harsh climatic conditions towards the top of the mountain interacts withother environmental factors at regional and local scale to influence the spatialvariation in local species composition and biodiversity maintenance. We analysedthe altitudinal variation in the taxonomic composition of epigaeic beetle assemblagesacross five mountains in north-western Patagonia (Argentina) to address whethersubstantial change in species composition was associated (i) at regional spatial scale,with changes in vegetation types, and the presence of dry and moist mountains, and(ii) at local spatial scale, with variation in temperature, plant cover and richness andseveral soil characteristics.2. We collected beetles using 486 pitfall traps arranged in fifty-four 100-m2 gridplots of nine traps settled at about 100 m of altitude apart from each other, from thebase to the summit of each mountain. We used multivariate analyses to identifybeetle assemblages and to evaluate their association with environment.3. We identified different beetle assemblages, associated more with vegetationtypes rather than with mountains; indicator species showed higher degree of fidelityand specificity to vegetation types rather than to mountains. Local variation intemperature, plant cover and richness, and soil characteristics influence the variationin species composition.4. Our study suggests the existence of a regional beetle fauna that is shared acrossthese mountains. Major regional changes in vegetation types and local variation inenvironment drive the variation in the species composition of beetle assemblages atthese latitudes.","palabras_clave":"altitud|coleópteros|especies indicadoras|Andes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62961,"idioma_id":4,"titulo":"Traditional horticultural and gathering practices in two semi-rural populations of Northwestern Patagonia","resumen":"Horticultural and gathering practices were studied in Comallo and Pilcaniyeu, two semi-rural communities  which share a common Mapuche ancestry and occupy similar arid environments in Northwest  Patagonia, Argentina. We analyzed cultivation and wild plant gathering using semi-structured interviews.  We hypothesized that these activities will be conditioned by access to market economy. Results  showed differences in horticultural and gathering patterns in both populations: the community with less  access to market economy presented greater richness of cultivated and gathered species and collected  more seeds from their own harvests, thus promoting germplasm conservation and higher levels of seed  exchange than the community that had more contact with urban centers. In these populations the  integration of ancestral and novel knowledge seems to favor flexibility and autonomy, which might help  locals deal with change. This could foster greater individual and collective wellbeing, as well as socioecological  diversity.","palabras_clave":"RESILIENCE|ETHNOBIOLOGY|EMBODIED COGNITION|PLASTICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62962,"idioma_id":4,"titulo":"Influence of structural features of tri-functionalized aryl phosphates on the outcome of the SRN1 process with stannyl anions. A DFT study","resumen":"Under irradiation, 1,3-bis(diethoxyphosphoryloxy)-4-chlorobenzene (2), 1,4-bis(diethoxyphosphoryloxy)-  3-chlorobenzene (3) and 1,3-bis(diethoxyphosphoryloxy)-5-chlorobenzene (4) react with trimethyltinsodium  (1) in liquid ammonia giving entirely different distribution of stannylated products. These differences  are explained through theoretical DFT studies. Experimental evidence for the involvement of an SRN1  mechanism was obtained","palabras_clave":"STANNYL ANIONS|ARYL PHOSPHATES|DFT STUDY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62963,"idioma_id":4,"titulo":"Ultra-Compact Dwarfs around NGC 3258 in the Antlia cluster","resumen":"The first compact stellar systems with luminosities in the range of ultracompact dwarfs (UCDs), discovered in the Antlia galaxy cluster, are presented in this paper. By means of imaging from VLT (FORS1), CTIO (MOSAIC), and the HST (ACS) archive, eleven UCDs\/bright globular clusters (GCs) are selected on the basis of photometry and confirmed as Antlia members through radial velocities measured on new GEMINI (GMOS-S) spectra. In addition, nine UCD candidates are identified taking into account properties derived from their surface brightness profiles. All of them, members and candidates, are located in the proximity of NGC 3258, one of the two brightest elliptical galaxies in the cluster core. Antlia UCDs in this sample present absolute magnitudes fainter than Mv ~ -11.6 mag and most of them have colours within the blue GC range, falling only two within the red GC range. Effective radii measured for the ones lying on the ACS field are in the range Reff = 3 - 11 pc and are similar to equivalent objects in other clusters, obtained from the literature. The UCD sample shares the same behaviour on the size-luminosity plane: a linear relation between Reff and Mv is present for UCDs brighter than Mv ~&nbsp; -10.5 -- -11 mag while no trend is detected for fainter ones, that have an approximately constant Reff . The projected spatial distribution of UCDs, GCs and X-ray emission points to an ongoing merger between two Antlia groups, dominated by NGC 3258 and NGC 3268. Nuclei of dwarf elliptical galaxies and blue UCDs share the same locus on the colour-magnitude diagram, supporting the hypothesis that someblue UCDs may be remnants of stripped nucleated dwarfs.","palabras_clave":"GALAXIES|CLUSTERS|ANTLIA |DWARF|NGC3258|NUCLEI|PHOTOMETRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62964,"idioma_id":4,"titulo":"Multicolour Metallicity Relations from Globular Clusters in NGC 4486 (M87)","resumen":"We present Gemini griz photometry for 521 globular cluster (GC) candidates in a 5.5 × 5.5 arcmin field centred 3.8 arcmin to the south and 0.9 arcmin to the west of the centre of the giant elliptical galaxy NGC 4486. All these objects have previously published (C-T1) photometry. We also present new (C-T1) photometry for 338 globulars, within 1.7 arcmin in galactocentric radius, which have (g-z) colours in the photometric system adopted by the Virgo Cluster Survey of the Advanced Camera for Surveys of the Hubble Space Telescope (HST). These photometric data are used to define a self-consistent multicolour grid (avoiding polynomial fits), including the optical (B-I) (Johnson system) and the (I-H) infrared (HST system) indices, and tentatively calibrated in terms of two chemical abundance scales. The resulting multicolour colour-chemical abundance relations are used to test GC chemical abundance distributions. This is accomplished by modelling the ten GC colour histograms that can be defined in terms of the C-griz bands. The results show that the best fit to the GC observed colour histograms is achieved by adopting a genuinely bimodal chemical abundance distribution NGC (Z). In this approach, each ('red' and 'blue') GC subpopulation follows a distinct colour-colour relation.","palabras_clave":"STAR|CLUSTERS|GALAXIES|HALOES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62965,"idioma_id":5,"titulo":"Primera aproximación a una interpretación materialista de la historia  argentina: \u201CAportes para una historia de la cultura en Argentina\u201D de  Germán Avé-Lallemant (1890)","resumen":"El 'padre' del marxismo argentino fue un inmigrante alemán: el agrimensor e ingeniero de minas Germán Avé-Lallemant (1835-1910). En 1890, Lallemant ofreció un análisis materialista pionero de la historia de su país adoptivo, que analizaba las causas del atraso argentino. Las razones que explican el estancamiento nacional se remontarían a la herencia retrógrada de la&nbsp; colonización española y a su supervivencia en la clase dominante local. Esta edición crítica de dicho estudio, inédito hasta hoy en castellano, se propone contribuir a la superación de visiones estereotipadas sobre el marxismo de Lallemant y de la Segunda Internacional.","palabras_clave":"Avé-Lallemant, Germán|Verein Vorwärts|Vorwärts (revista)|historia del socialismo argentino"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62966,"idioma_id":4,"titulo":"Optical Properties of Silver-Coated Silicon Nanowires: Morphological and Plasmonic Excitations","resumen":"The optical extinction spectra of micro- andnanoparticles made up of high-contrast dielectrics exhibita set of very intense peaks due to the excitations ofmorphology-dependent resonances (MDRs). These kind ofresonances are well known at the microscopic scale as whis-pering gallery modes. In this work, we study numericallythe optical spectra corresponding to a core?shell structurecomposed by an infinite silicon nanowire coated with asilver shell. This structure shows a combination of bothexcitations: MDRs and the well-known surface plasmonresonances in dielectric metallic core?shell nanoparticles(Ekeroth Abraham and Lester, Plasmon 2012) . We com-pute in an exact form the complete electromagnetic responsefor both bare and coated silicon nanowires in the range of24?200 nm of cross-sectional sizes. We take into accountan experimental bulk dielectric function of crystalline sil-icon and silver by using a correction by size of the metaldielectric function. In this paper, we consider small silvershells in the range of 1?10 nm of thickness as coatings.We analyze the optical response in both the far and nearfields, involving wavelengths in the extended range of 300?2,400 nm. We show that the MDRs excited at the coreare selectively perturbated by the metallic shell throughthe bonding and antibonding surface plasmons (SPs). Thisperturbation depends on both the size of the core and thethickness of the shell, and, as a consequence, weget an efficient tuneable and detectable simple sys-tem. Our calculations apply perfectly to long nanotubescompared to the wavelength for the two fundamentalpolarizations (s, p).","palabras_clave":"CORE-SHELL NANOWIRES|PLASMONICS|MOPHOLOGIY-DEPENDENT RESONANCES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62967,"idioma_id":4,"titulo":"Cynara cardunculus L. as a potential industry crop","resumen":"Fourteen Cynara cardunculus L. accessions were compared in order to evaluate fresh total aboveground biomass production as well as its partition into leaves, stalks and capitula at the anthesis stage. For each botanical variety, percentages of dry matter were also calculated. An ANOVA was performed and mean values were compared by Duncan?s test. Euclidean distances were calculated and a cluster analysis was performed. Total fresh biomass and all its components showed significant differences among accessions","palabras_clave":"GLOBE ARTICHOKE|CULTIVATED CARDOON|WILD CARDOON|ABOVEGROUND BIOMASS|BIOMASS PARTITIONING|DRY MATTER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62968,"idioma_id":4,"titulo":"Development of a C. cardunculus Linkage Map Based on SRAP, SSR, SNP Markers and Localization of Phenotypic Traits","resumen":"In order to develop a new linkage map of C. cardunculus L., an F1 mapping population was generated from an intraspecific cross between a local genotype of wild cardoon and the globe artichoke ´Estrella del Sur FCA´. The population was genotyped using a combination of molecular markers and 3 phenotypic traits. A total of 421 segregating loci were identified. The female parent map (988.09 cM) consisted in 127 loci, spread over 15 linkage groups (LGs), while the male one (578.2 cM) resulted in 112 loci, distributed in 10 major LGs. The alignment between the female and male maps, by using intercross markers, allowed to determine 17 major LGs. The phenotypic traits Color Head and Spine Leaf were included inthe wild cardoon map, whereas Spine Head was positioned on the LG7 globe artichoke map. The presence of 48 loci in common between the present maps and the previously developed SSR-based linkage maps of the Cynara cardunculus species, allowed the alignment of the LGs. The developed maps represent important tools for the localization of interesting agronomic traits as well as for molecular-assisted breeding strategies and will be useful for the construction of an integrated map and for comparative genetics analysis.","palabras_clave":"Artichoke|Will cardoon|Molecular markers|Color head, spine leaf, spine head"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62969,"idioma_id":4,"titulo":"Role of CaMKII in post acidosis arrhythmias: A simulation study using a human myocyte model","resumen":"Postacidotic arrhythmias have been associated to increased sarcoplasmic reticulum (SR) Ca2+ load and Ca2+\/calmodulin-dependent protein kinase II (CaMKII) activation. However, the molecular underlying mechanisms of these arrhythmias are still unclear. To better understand this process, acidosis effects were incorporated into a myocyte model of excitation-contraction coupling and contractility, including acidotic inhibition of L-type Ca2+ channel (ICaL), Na+-Ca2+ exchanger, Ca2+ release through SR ryanodine receptor (RyR2) (Irel), Ca2+ reuptake by the SR Ca2+ ATPase2a (Iup), Na+-K+ pump, K+ efflux through the inward rectifier K+ channel and the transient outward K+ flow together with increased activity of the Na+-H+ exchanger (INHE). CaMKII ion flow facilitation affecting Irel, Iup, ICaL, INHE was introduced in the model to partially compensate the acidosis outcome. Late INa increase by CaMKII was also incorporated. Using this scheme and assuming that diastolic Ca2+ leak through RyR2 was modulated by the resting state of this channel and the difference between SR and dyadic cleft [Ca2+], postacidotic delayed afterdepolarizations (DADs) were triggered upon returning to normal pH after a 6 min acidotic period at pH = 6.7. The model showed that DADs depend on SR Ca2+ load and on increased Ca2+ leak through RyR2. This postacidotic arrhythmogenic pattern relies mainly on CaMKII effect on ICaL, Iup, and INHE since their individual elimination had the greatest incidence in DAD occurrence. The model further revealed that in the transition period to normal pH, DADs are fully determined by SR Ca2+ load at the end of acidosis. During postacidosis, DADs are maintained by SR Ca2+ reloading by Ca2+ influx through the reverse NCX mode during the time period in which [Na+]i is elevated. ","palabras_clave":"myocyte model|acidotic arrhythmogenesis|sarcoplasmic reticulum Ca2+ leak"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62970,"idioma_id":4,"titulo":"Microencapsulation of linseed oil by spray drying for functional food application","resumen":"Health benefits associated to &#969;-3 fatty acids consumption together with the high susceptibility to oxidation of &#969;-3 containing oils have led to the development of microencapsulated oils for nutraceutical and food enrichment applications. The aim of this work is to obtain different formulations for linseed oil microencapsulation by spray-drying with high encapsulation efficiency and evaluate their resistance to oxidation through the accelerated Rancimat test. Four formulations were tested; using different combinations of gum arabic (GA), maltodextrin (MD), methyl cellulose (MC) and whey protein isolate (WPI). Microcapsules made of 100% GA and ternary mixtures of GA, MD and WPI presented the highest protection from oxidation and microencapsulation efficiencies higher than 90%. They also presented spherical structures with smooth surfaces which kept unaltered after 10-month storage. GA containing formulation was included in bread manufacturing. Fortified bread resulted similar in appearance to control bread without microcapsules, but &#945;-linolenic acid content was reduced significantly after preparation.","palabras_clave":"Linseed oil|Microencapsulation|Rancimat test|Functional bread"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62971,"idioma_id":4,"titulo":"From neural signatures of emotional modulation to social cognition: Individual differences in healthy volunteers and psychiatric participants","resumen":"It is commonly assumed that early emotional signals provide relevant informationfor social cognition tasks. The goal of this study was to test the association between (a) cortical markers of face emotional processing and (b) social-cognitive measures,and also to build a model which can predictthis association (a &amp; b) in healthy volunteers as well as in different groups of psychiatric patients. Thus, we investigated the early cortical processing of emotional stimuli (N170, using a face and word valence task) and their relationship with the social-cognitive profiles (SCPs, indexed by measures of theory of mind, fluid intelligence, speed processing, and executive functions). Group comparisons and individual differences were assessed among schizophrenia (SCZ) patients and their relatives, individuals with attention deficit hyperactivity disorder (ADHD), individuals with euthymic bipolar disorder (BD) and healthy participants (educational level, handedness, age and gendermatched). Our results provide evidence of emotional N170 impairments in the affected groups (SCZ and relatives, ADHD and BD) as well as subtle group differences. Importantly, cortical processing of emotional stimuli predicted the social cognition profile (SCP), as evidenced by a structural equation model (SEM) analysis. This is the first study to report anassociation model of brain markers of emotional processing and SCP.","palabras_clave":"N170|SEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62972,"idioma_id":4,"titulo":"Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in Pseudomonas extremaustralis","resumen":"Therole of Anr in oxidative stress resistance was investigated in Pseudomonas extremaustralis, apolyhydroxybutyrate-producing Antarctic bacterium. The absence of Anr causedincreased sensitivity to hydrogen peroxide under low oxygen tension. Thisphenomenon was associated with a decrease in the redox ratio, higher oxygenconsumption and higher reactive oxygen species production. Physiologicalresponses of the mutant to the oxidized state included an increase in NADP(H)content, catalase activity and exopolysaccharide production. The wild-typestrain showed a sharp decrease in the reduced thiol pool when exposed tohydrogen peroxide, not observed in the mutant strain. In silico analysis of the genome sequence of P. extremaustralisrevealed putative Anr binding sites upstream from genes related to oxidativestress. Genes encoding several chaperones and cold shock proteins, aglutathione synthase, a sulfate transporter and a thiol peroxidase wereidentified as potential targets for Anr regulation. Our results suggest a novelrole for Anr in oxidative stress resistance and in redox balance maintenanceunder conditions of restricted oxygen supply.                  ","palabras_clave":"PSEUDOMONAS EXTREMAUSTRALIS|Anr|GLOBAL REGULATORY NETWORKS|REDOX HOMEOSTASIS|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62973,"idioma_id":4,"titulo":"The paucity of globular clusters around the field elliptical NGC 7507","resumen":"Context. There is strong evidence that globular cluster systems (GCSs) of massive galaxies are largely assembled by infall\/accretion processes. Therefore, we expect the GCSs of isolated elliptical galaxies to be poor. Although not completely isolated, NGC 7507 is a massive field elliptical galaxy with an apparently very low dark matter content. Aims: We determine the richness, the colour distribution, and the structural properties of the GCS of NGC 7507. Methods: We performed wide-field Washington photometry with data obtained with the MOSAIC II camera at the 4m-Blanco telescope (CTIO, Chile). Results: The GCS is very poor with SN &#8776; 0.6. We identify three subpopulations with peaks at (C - T1) colours of 1.21, 1.42, and 1.72. The bluest population may represent the old, metal-poor component. This interpretation is supported by its shallow density profile. The red population is more concentrated, resembling the galaxy light. The intermediate-colour population is strongly peaked in colour, and we interpret this population as the signature of a starburst whose age depends on the metallicity, but should be quite old, since no signatures of a merger are identifiable. In addition, we find a main sequence in the stellar foreground population, which we attribute to the Sagittarius dwarf tidal stream. Conclusions: The extrordinarily poor GCS of NGC 7507, a massive elliptical galaxy, is an illustration of how important the environmental conditions are for producing rich GCSs.","palabras_clave":"GALAXIES|ELLIPTICAL|CLUSTERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62974,"idioma_id":4,"titulo":"Nonlinear dynamic vibration absorbers with a saturation","resumen":"The behavior of a new type of nonlinear dynamic vibration absorber is studied. A distinctive characteristic of the proposed absorber is the impossibility to extend the system to infinity. The mathematical formulation is based on a finite extensibility nonlinear elastic potential to model the saturable nonlinearity. The absorber is attached  to a single degree-of-freedom linear\/nonlinear oscillator subjected to a periodic external excitation. In order to solve the equations of motion and to analyze the frequency response curves, the method of averaging is used. The performance of the FENE absorber is evaluated considering a variation of the nonlinearity of the primary system, the damping and the linearized frequency of the absorber and the mass ratio. The numerical results show that the proposed absorber has a very good efficiency when the nonlinearity of the primary system increases. When compared with a cubic nonlinear absorber, for a large nonlinearity of the primary system, the FENE absorber shows a better effectiveness for the whole studied frequency range. A complete absence of quasi-periodic oscillations is also found for an appropriate selection of the parameters of the absorber. Finally, direct integrations of the equations of motion are performed to verify the accuracy of the proposed method.","palabras_clave":"vibration absorber|Nonlinear dynamic|saturation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62975,"idioma_id":4,"titulo":"Nonlinear dynamics of rotating box FGM beams using nonlinear normal modes","resumen":"In this work an analysis is performed on the nonlinear planar vibrations of a functionally graded beam subjected to a combined thermal and harmonic transverse load in the presence of internal resonance.  Adopting the direct perturbation MMS technique, the partial differential equations of motion of the beam are reduced to sets of first-order nonlinear modulation equations in terms of the complex modes  of the beam. The assumption of steady-state values of centrifugal loads is evaluated. It has to be said that there is a lack of information about modeling of rotating box beams made of functionally graded  materials (FGMs) under thermo-mechanical loads. The influence of the transverse load amplitude and the internal detuning parameter on the strength of nonlinear modal interaction is illustrated. It is also  shown that the system exhibits periodic and quasiperiodic responses for a typical range of parameter values.","palabras_clave":"Nonlinear dynamics|FGM|Internal resonance|Nonlinear modes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62976,"idioma_id":4,"titulo":"Reconstruction of the Early Ordovician Famatinian arc through thermobarometry in lower and middle crustal exposures, Sierra de Valle Fértil, Argentina","resumen":"The crustal structure of the Famatinian paleoarc is reconstructed by determining the metamorphic crystallization P-T conditions from metasedimentary rocks at various structural levels in the Valle Fértil section. The bulk section exhibits a 15-km-thick arc crustal section. Thermobarometry shows that nested tonalitic and granodioritic plutons constructed the arc crust at depths &lt; 20 km. Dioritic and tonalitic bodies dominated between 20 and 25 km. The deepest exposed paleodepths are formed by cumulate and non-cumulate gabbroic rocks with interlayered quartz diorites that crystallized below 25 km. The boundary between the crust and the lithospheric mantle is not observed and would have been underneath a depth of 27 km. P-T estimates throughout the section reveals high geothermal gradients ranging from 25 °C\/km to 35 °C\/km. The thermal regime inferred for middle crustal levels is observed in active and ancient magmatic arcs. Thermophysical models predicting the array of retrieved P-T estimates require heat advection conducted by mafic magmas which either emplaced in the lower crust and\/or intruded into middle crustal levels. Calculated seismic wave velocities of plutonic rocks dominating progressively deeper paleodepths are used to deduce the internal architecture of the Valle Fértil section. This result indicates that the Famatinian arc had a middle crustal structure very similar to that of the ancient Talkeetna arc; however a rapid increase of seismic wave velocities from ~ 6.3 km\/s to &gt; 6.6 km\/s is located at deeper depths in the Famatinian arc than in Talkeetna arc. The thickness of a crustal layer dominated by plutonic rocks with low seismic wave velocities (&lt; 6.2 km\/s) is 10 km thinner than the crustal layer with similar physical properties in the Sierra Nevada batholith. A putative model for the whole Famatinian arc suggests a total crustal thickness between 30 and 35 km with three distinct layers.","palabras_clave":"Paleoarcs   |Thermobarometry|Subduction zones|Famatinian arc"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62977,"idioma_id":5,"titulo":"Geología de la Sierra de Chávez en el sector Centro-Occidental de la Sierra de Valle Fértil, San Juan: Metamorfismo y Evolución Tectónica de la Corteza Inferior del Arco Famatiniano","resumen":"Geology of the Sierra de Chávez in central-western section of the Sierra de Valle Fértil, San Juan: metamorphism and tectonic evolution in the lower crust of the Famatinian arc.  The geology of the Sierra the Chavéz is constituted by igneous and metamorphic rocks that appear complexly inter-layered at all scales, and belong to the mafic unit from defined in the Sierra de Valle Fértil. Five lithological associations have been identified in this complex, which are: dioritic, gabbroic, metasedimentary, mylonitic and granitic-pegmatitic. Thermobarometric estimates performed in metasedimentary migmatites yield peak (M1) metamorphic conditions of 876 °C and 8.3 Kbar, and are interpreted to reflect Early Ordovician magmatism (~ 489-470 Ma). Maximum estimated metamorphic pressures correspond to one of the deepest crustal paleo-depths found all over the Famatinian arc. Field observations and structural analyses allow recognizing the presence of several magmatic and tectonic fabrics, which are: i) magmatic layering (S0) interpreted as primary magmatic foliation, ii) sub-magmatic to sub-solid high temperature foliation and metamorphic foliation (S1), iii) mylonitic foliations first formed in amphibolite (S2a) and then re-worked under green schist facies (S2b), and iv) brittle foliation (S3) occuring as centimeter-spaced cleavage fracture. Both (S0) and (S1) foliations are associated to a first stage of deformation (D1) that developed at high temperature conditions (granulite facies) and was broadly coeval with the magmatism and the metamorphic peak (M1). (S2a,b) and (S3) structures transposes the igneous and metamorphic fabrics developed during the D1 event, and are related to a second and third stage of deformation (D2-D3) which represent a progressive evolution of the deformation from ductile to fragile regimens related to the stage of closure and exhumation of the Famatinian arc.","palabras_clave":"Sierra de Chávez,|arco Famatiniano,|complejo máfico,| evolución petrológica estructural."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62978,"idioma_id":5,"titulo":"La obesidad materna y la programación del fenotipo obeso","resumen":"La prevalencia de la obesidad se ha incrementado y extendido a toda la población sin distinción de edad, sexo, etnia o clase sociocultural. Año a año la cifra de adultos con sobrepeso y obesidad se incrementa, así como la incidencia de la morbilidad asociada. La abundancia de comidas rápidas, hipercalóricas y relativamente económicas, junto con el estilo de vida sedentario, han colaborado con el desarrollo del fenotipo obeso. Por otro lado y de manera alarmante, existe un aumento en la incidencia de la obesidad en niños menores de cinco años. En un intento de hallar la causa de este incremento, se ha estudiado y observado que los hijos de madres obesas presentan mayor incidencia de obesidad y sobrepeso, lo que indica que la obesidad es una alteración metabólica que se transmite de generación en generación. Se ha establecido que la exposición a un entorno metabólico alterado como la obesidad en etapas críticas del desarrollo ?programa? alteraciones en los distintos órganos y sistemas que impactarán en el metabolismo del individuo durante el resto de su vida. En esta revisión se analizarán algunos de los diferentes mecanismos que se alteran durante el desarrollo y conforman las bases para el desarrollo del fenotipo obeso en la adultez.","palabras_clave":"OBESIDAD|PROGARMACION PERINATAL|ALTERACIONES METABOLICAS|SOBREPESO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62979,"idioma_id":4,"titulo":"Participation of eIF4F complex in Junin virus infection: blockage of eIF4E does not impair virus replication","resumen":"Translation efficiency of viral mRNAs is a key factor defining both cytopathogenicity and virulence of viruses, which are entirely dependent on the cellular translation machinery to synthesize their proteins. This dependence has led them to develop different translational reprogramming strategies to ensure viral mRNAs can effectively compete with cellular mRNAs. Junin virus (JUNV) is a member of the family Arenaviridae, whose mRNAs are capped but not polyadenylated. In this work we evaluated the relevance to JUNV replication of the main components of the eIF4F complex: eIF4A, eIF4GI and eIF4E. We found the viral nucleoprotein (N) of JUNV colocalized with eIF4A and eIF4GI but not with eIF4E. Moreover, N could be immunoprecipitated in association with eIF4A and eIF4GI but not with eIF4E. Accordingly, functional impairment of eIF4A as well as eIF4GI reduced JUNV multiplication. By contrast, inhibition of eIF4E did not show a significant effect on JUNV protein synthesis. A similar situation was observed for another two members of arenaviruses: Tacaribe (TCRV) and Pichinde (PICV) viruses. Finally, the nucleoproteins of JUNV, TCRV and PICV were able to interact with 7 methyl-guanosine (cap), suggesting that the independence of JUNV multiplication on eIF4E, the cap-binding protein, may be due to the replacement of this factor by N protein.","palabras_clave":"Virus Junin|nucleoproteina|eIFs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62980,"idioma_id":10,"titulo":"Narrativas no estudo das práticas em saúde mental: contribuições das perspectivas     de Paul Ricoeur, Walter Benjamim e a antropologia médica","resumen":"Narrativas   são cada vez mais frequentes em estudos qualitativos para compreender   experiências e diferentes visões de sujeitos num dado contexto. Partindo   desta concepção, faz-se o resgate de tradições que abordam a   narratividade - a filosofia de Paul Ricoeur, a perspectiva histórica em   Walter Benjamin e o campo da antropologia médica constituída a partir da   fenomenologia. Em Ricoeur, tendo a hermenêutica como pensamento   derivado e variante da fenomenologia, a narrativa é ligada à   temporalidade. Em Benjamin, a narrativa, sempre inconclusa, feita de   restos e fragmentos, emerge à revelia das histórias oficiais. Se Ricoeur   retoma de Gadamer a tradição como componente fundamental para a   construção de um mundo do texto com que se torna possível a imitação da   vida, Benjamin, diante da derrocada da tradição, aponta para a invenção   de formas narrativas fora dos cânones tradicionais, possibilitando   retomar o passado para transformar o presente. Apresentam-se ainda   pressupostos da antropologia médica, que considera a narrativa como   dimensão do vivido e não sua abstração, ou seja, uma narrativa   corporificada e situada. Por fim, apresentam-se três pesquisas distintas   em saúde mental que se utilizam de narrativas, articuladas às correntes   teóricas apresentadas, com suas diferenças e aproximações.","palabras_clave":"Narrativas|Salud Mental|Antropologia Médica|Paul Ricoeur|Walter Benjamin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62981,"idioma_id":4,"titulo":"A Distributed Repository for Managing Business Process Models in Cross-Organizational  Collaborations","resumen":"Cross-organizational collaborations require the management of models for: collaborative business processes (CBPs), which define the collaboration´s behavior; and integration business processes (IBPs), which define the behavior that supports the role an organization performs in a CBP. Managing these business process models becomes a complex task when organizations integrate collaborative networks and set up several cross-organizational collaborations. This paper presents a distributed repository that provides the functionalities required to manage conceptual business process models involved in cross-organizational collaborations. A service-oriented architecture is proposed for the distributed repository. This architecture enables organizations to access a global repository for managing collaborative networks, cross-organizational collaborations, and their CBP models. Organizations can also maintain local repositories of IBP models, which are synchronized and consistent with CBP models, while preserving their private aspects. By using verification methods and a model-driven architecture method, the distributed repository provides services that support the synchronization, consistency and interoperability requirements for CBP and IBP models. A case study is presented along with an implementation of the distributed repository.","palabras_clave":"Cross-organizational Collaboration|Collaborative Business Process|Integration Business  Process|Distributed Business Process Model Repository"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62982,"idioma_id":4,"titulo":"Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes","resumen":"Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in bovinefollicular fluid; when added to maturation culture media, it affects oocyte competence(depending on the type and concentration of LA used). To date, little is known about theeffective level of incorporation of LA and there is apparently no information regarding itsesterification into various lipid fractions of the oocyte and its effect on neutral lipid storage.Therefore, the objective was to assess the uptake and subcellular lipid distribution of LA byanalyzing incorporation of radiolabeled LA into oocyte polar and neutral lipid classes. Theeffects of various concentrations of LA on the nuclear status and cytoplasmic lipid content ofbovine oocytes matured in vitro was also analyzed, with particular emphasis on intermediateconcentrations of LA.Neutral lipids stored in lipid dropletswere quantified with a fluorescenceapproach. Linoleic acid at 9 and 43 uM did not affect the nuclear status of oocytes maturedin vitro, and 100 uM LA inhibited germinal vesicle breakdown, resulting in a higher percentageof oocytes arrested at the germinal state (43.5 vs. 3.0 in controls; P &lt; 0.05). Bovine oocytesactively incorporated LA from the maturation medium (83.4 pmol LA per 100 oocytes at 22hours of incubation; P &lt; 0.05) and metabolized it mainly into major lipid classes, e.g., triacylglycerols and phospholipids (61.1% and 29.3%, respectively). Supplementation of thematuration medium with LA increased triacylglycerol accumulation in cytoplasmic lipiddroplets at all concentrations assayed (P &lt; 0.05). In conclusion, LA added to a defined maturationmedium at concentrations that did not alter the nuclear status of bovine oocytesmatured in vitro (9 and 43 uM) improved their quality by increasing the content of neutrallipids stored in lipid droplets. By directing the free fatty acid (LA) to triacylglycerol synthesispathways and increasing the degree of unsaturation of membrane phospholipids, the oocytewas protected from lipotoxic effects (with an expectation of improved cryotolerance).","palabras_clave":"LINOLEIC ACID|IN VITRO MATURATION|LIPID DROPLETS|OOCYTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62983,"idioma_id":4,"titulo":"Platinum nanoparticles produced by EG\/PVP method: The effect of cleaning on the electro-oxidation of glycerol","resumen":"Carbon supported Pt nanoparticles (NPs) have been prepared through polyols method using PVP. As observed with a large number of surfactants employed in NPs synthesis, it is very difficult to attain an efficient NPs cleaning, which is a fundamental requirement in catalysis researches. The aim of this paper was to obtain clean NPs, beginning by using \u201Csoft\u201D methods, in order to keep unaltered both the NPs size and shape; however, an important surfactant remnant was observed. Consequently, it was performed an electrochemical cleaning through PVP and glycolate electro-oxidation, being accompanied with important perturbations of NPs atoms and dissolution and re-deposition of Pt. Finally, the performance of thecatalyst towards the glycerol electro-oxidation reaction (GER), before and after the cleaning process was studied. In addition, we demonstrate how the impurities bring about poor reproducibility in this kind of experiments due to the fact that the cleaning process is occurring at the same time that the GER and because cleaning extent along cycles varies for each independent experiment.","palabras_clave":"Platinum nanoparticles| Ethylene glycol synthesis |Surfactant removal |Glycerol electro-oxidation reaction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62984,"idioma_id":4,"titulo":"Archaeocyaths from South America: review and a new record","resumen":"In South America, autochthonous archaeocyathan faunas preserved in Early Cambrian limestones have not been found yet. Nevertheless, a few  well-documented occurrences of these fossils in clasts contained in coarse-grained rocks of a wide age range have been discovered in recent years.  Erratic limestone blocks from the Late Carboniferous?Early Permian Fitzroy Tillite Formation in the Falkland\/Malvinas Islands yielded three  archaeocyath taxa. Also, seven taxa were reported from archaeocyathan limestone clasts in a metaconglomerate of the Cambro-Ordovician El  Jagüelito Formation in northern Patagonia. In addition, a new record from the Late Carboniferous?Early Permian Sauce Grande Formation  diamictites in Sierras Australes, Buenos Aires Province, Argentina, is presented herein. Preservation of this scarce new material is poor, but at least  three different taxa can be distinguished. The most likely source of all archaeocyathan limestone clasts found in southern South America is the  Shackleton Limestone from the Transantarctic Mountains in East Antarctica. The new record from the Sauce Grande Formation and the  inferred clast provenance reinforce the correlation between this unit, the Dwyka Tillite (South Africa) and the Fitzroy Tillite Formation  (Falklands\/Malvinas), suggesting a very wide distribution of these Antarctic occurrences during the Late Carboniferous?Early Permian  Gondwana glaciation (Episode III). Thus, even though being allochthonous, archaeocyaths are emerging as a new key biological feature  for Gondwana palaeogeographic reconstructions. Copyright © 2012 John Wiley & Sons, Ltd.","palabras_clave":"Archaeocyaths|South America|Gondwana|Late Palaeozoic glaciations|Sierras Australes|Buenos Aires"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62985,"idioma_id":4,"titulo":"The sesquiterpene lactone dehydroleucodine triggers senescence and apoptosis in association with accumulation of DNA damage markers","resumen":"Sesquiterpene lactones (SLs) are plant-derived compounds that display anti-cancer effects. Some SLs derivatives have a marked killing effect on cancer cells and have therefore reached clinical trials. Little is known regarding the mechanism of action of SLs. We studied the responses of human cancer cells exposed to various concentrations of dehydroleucodine (DhL), a SL of the guaianolide group isolated and purified from Artemisia douglasiana (Besser), a medicinal herb that is commonly used in Argentina. We demonstrate for the first time that treatment of cancer cells with DhL, promotes the accumulation of DNA damage markers such as phosphorylation of ATM and focal organization of &#947;H2AX and 53BP1. This accumulation triggers cell senescence or apoptosis depending on the concentration of the DhL delivered to cells. Transient DhL treatment also induces marked accumulation of senescent cells. Our findings help elucidate the mechanism whereby DhL triggers cell cycle arrest and cell death and provide a basis for further exploration of the effects of DhL in in vivo cancer treatment models.","palabras_clave":"Apoptosis|DNA Damage|Sesquiterpene lactones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62986,"idioma_id":4,"titulo":"Methoprene treatment reduces the pre-copulatory period in Anastrepha fraterculus (Diptera: Tephritidae) sterile males","resumen":"Anastrepha fraterculus is a major fruit pest in South America. Ongoing studies support the implementation of the sterile insect technique (SIT) against this pest. Sexual readiness of sterile males is a key point for SIT application. The pre-copulatory period of A. fraterculus males has not been reported before, but it is expected to last several days. An acceleration of sexual maturation was achieved in other Anastrepha species after topical applications of juvenile hormone analogues, like methoprene. Here, we studied the effect of methoprene on male sexual maturation, mating duration and sperm transfer in A. fraterculus as well as the impact of acetone (methoprene solvent) on survival. We also explored a method to deliver methoprene massively. Pheromone-calling and mating ability were evaluated daily from adult emergence, and used as indicators of sexual maturity. Anastrepha fraterculus males showed a long pre-copulatory period (7 days approximately), as other Anastrepha species. This process was accelerated after methoprene treatment (2.5 &#956;g\/&#956;l), both in non-irradiated and irradiated males which matured 2?3 days earlier. Mating duration for methoprene-treated males was longer than for mature untreated males, however, no differences in sperm transfer were detected. Survival was not affected by acetone. Dipping pupae in methoprene allowed emerging males to mature as fast as those receiving topical application as adults. Dipping of pupae is a promising method to deliver massively methoprene and should be further investigated.","palabras_clave":"JUVENILE HORMONE|SEXUAL MATURATION|SOUTH AMERICAN FRUIT FLY|SIT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62987,"idioma_id":4,"titulo":"Experimental and Theoretical Studies on Polar Diels-Alder Reactions of 1-Nitronaphathalene developed in Ionic Liquids","resumen":"The reactions between 1-nitronaphthalene 1 and two nucleophilic dienes, Danishefsky´s diene 2 and 1-trimethylsilyloxy-1,3-butadiene 3, to yield phenanthrenols 7 and 8 developed in protic and aprotic ionic liquids (ILs) with an imidazolium cation base permit to conclude that the reactions occur in better experimental conditions than in molecular solvents. The yields obtained with protic ILs are better than those observed with aprotic ILs. In this sense, the higher yields obtained with aprotic ILs, (working at 60 uC,48 h) are comparable with the lower yields observed in protic ILs (60 uC, 24 h). DFT calculations for the reaction of 1-nitronaphthalene 1 with diene 3, in the absence and in the presence of tetrafluoroborate of 1-metilimidazolium [HMIM][BF4] as protic ILs, indicate that this reaction is a domino process that comprises two consecutive processes: i) a polar Diels?Alder (P-DA) reaction between 1 and 3, and ii) an elimination of nitroso acid 5 to yield dihydrophenanthrene 11. Thermodynamic calculations indicate that while the first reaction is the rate-determining step of this domino process, the nitroso acid elimination is the driving force responsible for the formation of dihydrophenanthrene 11. Implicit and explicit solvent effects indicate that while the hydrogen bond formation between HMIM and one oxygen atom of the nitro groups favour the two reactions, implicit solvent effects modelled by PCM decelerate the P-DA reaction by a large solvation of reagents. Finally, DFT analysis of reagents offers an explanation of the catalytic role of protic ILs in these P-DA reactions.","palabras_clave":"DIELS-ALDER|IONIC LIQUIDS|NITRONAPHTALENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62988,"idioma_id":5,"titulo":": \u201CMECANISMOS DE TUMOROGENESIS HIPOFISARIA. ESTUDIO DE FACTORES ANGIOGÉNICOS Y DE PROLIFERACIÓN\u201D","resumen":"Introducción y Objetivos: La angiogénesis es un proceso fundamental en el desarrollo tumoral. Sin embargo, se han encontrado discrepancias en el patrón angiogénico de los tumores hipofisarios. Nos propusimos   estudiar la expresión de VEGF y FGF2 y su importancia en la vascularización de los adenomas hipofisarios,  cuantificar los vasos con los marcadores CD31 y CD34 y determinar el índice de proliferación con PCNA y Ki67.  Materiales y Métodos: Se estudiaron 76 macroadenomas hipofisarios que fueron intervenidos quirúrgicamente. Los adenomas se clasificaron según su secreción hormonal.   A partir de cortes histológicos se realizó inmunohistoquímica para los marcadores de endotelio CD31 y   CD34; y Ki-67 para estudio de proliferación celular. Por western blot se midieron VEGF, CD31 y PCNA. Se   efectuaron comparaciones con glándulas normales.  Resultados: El nivel de expresión de VEGF, hallado en todas las muestras analizadas, resultó mayor en los   prolactinomas resistentes respecto a los demás tipos de adenomas hipofisarios. Esta proteína localizó en las   células endoteliales de los vasos como así también en citoplasmas y núcleos de células tumorales.   El 56 % de las muestras resultaron positivas para FGF2, mostrando localización citoplasmática y en matriz   extracelular.  Obtuvimos una fuerte correlación positiva entre VEGF y CD31 en las muestras tumorales, sin encontrar   correlación lineal entre PCNA y VEGF, ni Ki-67 y VEGF en las muestras estudiadas.  El área vascular resultó mayor en los tejidos normales que en los tumores utilizando CD34 como marcador   de vasos.   Conclusión: La importancia del estudio de la angiogénesis en los adenomas hipofisarios radica en la necesidad de hallar marcadores moleculares que predigan el comportamiento tumoral.  Pudimos demostrar la expresión de los factores angiogénicos VEGF y FGF2 en estos adenomas, y la existencia   de correlación lineal entre VEGF y CD31. Nuestros resultados son indicativos de existencia de angiogénesis   en los adenomas hipofisarios por lo que su bloqueo podría plantearse como una estrategia alternativa para   los casos resistentes a las terapias convencionales","palabras_clave":"Adenomas hipofisarios|Angiogènesis|VEGF|proliferación celular"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62989,"idioma_id":4,"titulo":"VALIDATION OF THE FALL3D MODEL FOR THE 2008 CHAITEN ERUPTION USING FIELD AND SATELLITE DATA","resumen":"      The 2008 Chaitén volcano eruption began on 2 May 2008 with an explosive phase that injected great amounts of tephra into the atmosphere. During the first week of the eruption, volcanic ash clouds spread for hundreds of kilometres over Argentina following the prevailing western winds. Tephra deposition extended to the Atlantic Ocean and severely affected the Argentinean Patagonia. Impacts included air and water quality degradation, disruption of ground transportation systems and cancellation of flights even at airports around 1500 km apart. We use the fall3d tephra transport model coupled with the Weather Research and Forecasting-Advanced Research Weather (WRF-ARW) meteorological model to simulate the 2-9 May eruptive period. Our hindcast results are in good agreement with satellite imagery and reproduce ground deposit observations. Key aspects of our analysis, not considered during syn-eruptive forecasts, are the re-initialization of each simulation with actualized meteorological forecast cycles and better constrained model inputs including column heights (inferred from reanalysis of GOES-10 imagery and nearby atmospheric soundings) and granulometric data obtained from field campaigns. This study shows the potential of coupling WRF\/ ARW and fall3d models for short-term forecast of volcanic ash clouds. Our results highlight that, in order to improve forecasting of ash cloud dispersion and tephra deposition, it is essential to implement an operational observation system to measure temporal variations of column height and granulometric characteristics of tephra particles in nearly real-time, at proximal as well as distal locations ","palabras_clave":"CHAITEN ERUPTION|FALL 3D MODEL|ASH DISPERSION|GROUND DEPOSITION |RISK MANAGEMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62990,"idioma_id":4,"titulo":"An improved DNA isolation technique for PCR detection of Strongyloides stercoralis in stool samples","resumen":"Strongyloides stercoralis is a nematode that causes severe infections in immunocompromised patients.  The low parasitic burden of chronically infected patients makes diagnosis difficult to achieve by conventional  methods. Here, an in-house (IH) method for the isolation of parasite DNA from stools and a  PCR assay for the molecular diagnosis of S. stercoralis were optimized. DNA yield and purity improved  with the IH method which included a step of incubation of stool samples with a glycine\u2013SDS buffer and  mechanical disruption prior to DNA extraction. For the PCR assay, the addition of bovine serum albumin  was required to neutralize inhibitors present in stool. The analytical sensitivity of the PCR using DNA as  template, isolated with the IH method, was superior to the commercial one. This study demonstrates that  a combined method that adds the step of glycine\u2013SDS buffer incubation plus mechanical disruption prior  to DNA isolation with the commercial kit increased PCR sensitivity to levels of the IH method. Finally, our  assay was tested on 17 clinical samples. With the IH method for DNA isolation, a S. stercoralis specific band  was detected by PCR in the first stool sample in all patients (17\/17), while with the commercial kit, our S.  stercoralis-specific band was only observed in 7 samples. The superior efficiency of the IH and combined  methods over the commercial kit was demonstrated when applied to clinical samples with low parasitic  burden. These results show that the DNA extraction procedure is a key to increase sensitivity of the S.  stercoralis PCR assay in stool samples. The method developed here could help to improve the molecular  diagnosis of S. stercoralis.","palabras_clave":"Strongyloides stercoralis|stool sampleDNA isolation|molecular diagnosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62991,"idioma_id":4,"titulo":"Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation","resumen":"Background: Previously, a bovine intestinal epithelial cell line (BIE cells) was successfully established. This work hypothesized that BIE cells are useful in vitro model system for the study of interactions of microbial- or pathogenassociated molecular patterns (MAMPs or PAMPs) with bovine intestinal epithelial cells and for the selection of immunoregulatory lactic acid bacteria (LAB).  Results: All toll-like receptor (TLR) genes were expressed in BIE cells, being TLR4 one of the most strongly expressed. We demonstrated that heat-stable PAMPs of enterotoxigenic Escherichia coli (ETEC) significantly enhanced the production of IL-6, IL-8, IL-1! and MCP-1 in BIE cells by activating both NF-'B and MAPK pathways. We evaluated the capacity of several lactobacilli strains to modulate heat-stable ETEC PAMPs-mediated inflammatory response in BIE cells. Among these strains evaluated, Lactobacillus casei OLL2768 attenuated heat-stable ETEC PAMPs-induced pro-inflammatory response by inhibiting NF-'B and p38 signaling pathways in BIE cells. Moreover, L. casei OLL2768 negatively regulated TLR4 signaling in BIE cells by up-regulating Toll interacting protein (Tollip) and B-cell lymphoma 3-encoded protein (Bcl-3).  Conclusions: BIE cells are suitable for the selection of immunoregulatory LAB and for studying the mechanisms involved in the protective activity of immunobiotics against pathogen-induced inflammatory damage. In addition, we showed that L. casei OLL2768 functionally modulate the bovine intestinal epithelium by attenuating heat-stable ETEC PAMPs-induced inflammation. Therefore L. casei OLL2768 is a good candidate for in vivo studying the protective effect of LAB against intestinal inflammatory damage induced by ETEC infection or heat-stable ETEC PAMPs challenge in the bovine host.","palabras_clave":"Bovine intestinal epithelial cells|immunobiotics|TLRs negative regulators|Lactobacillus casei OLL2768"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62992,"idioma_id":4,"titulo":"Nasally administered Lactobacillus rhamnosus accelerate the recovery of humoral immunity in B lymphocyte-deficient malnourished mice","resumen":"The ability of nasally administered Lactobacillus rhamnosus CRL1505 to accelerate the recovery of respiratory B cell- mediated immunity against pneumococcal infection in replete malnourished mice was evaluated. Weaned mice were malnourished after consumption of a protein-free diet for 21 d. Malnourished mice were fed a balanced conventional diet (BCD) for 7 d (BCD group) or a BCD for 7 d with supplemental L. rhamnosus CRL1505 by the nasal route during the last 2 d (BCD+Lr group). Nonreplete malnourished and normal mice were used as the malnourished (MNC) and the well-nourished (WNC) control groups, respectively. Mice were challenged with Streptococcus pneumoniae at the end of each dietary treatment. The immune response was studied before the challenge and at different times postinfection. The MNC mice had less resistance to pneumococcal infection, fewer mature and immature B cells in lung and spleen, and a reduced production of specific antibodies compared with WNC mice. The BCD treatment did not induce a complete normalization of the number B cell populations and antibody amounts. However, the BCD+Lr group had normal numbers of spleen and lung B cells. Moreover, the BCD+Lr mice had a significantly lower susceptibility to S. pneumoniae infection and higher amounts of anti-pneumococcal antibodies. Although further studies are necessary to clarify the effect of malnutrition and nasally administered lactobacilli in other immune cell populations involved in the protection against respiratory pathogens, this work gives evidence of the importance of using nasal priming with probiotics to accelerate the recovery of respiratory immunity in immunocompromised malnourished hosts.","palabras_clave":"respiratory immunity|malnutrition|B lymphocytes|Lactobacillus rhamnosus CRL1505"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62993,"idioma_id":4,"titulo":"Recent Advances on Nanotechnology Applications to Cancer Drug  Therapy","resumen":"One of the greatest challenges in cancer drug therapy is to maximize the effectiveness of the active ingredient while reducing its systemic adverse effects. Conventional (non-targeted) systemic drug therapy is characterized by unspecific distribution of the anticancer drugs: both healthy and affected tissues are thus exposed to the chemotherapeutic agent, giving raise to off-target side-effects. Besides, a number of widely-used chemoterapeutic agents present unfavorable physicochemical properties, such as low solubility or low stability issues, limiting their available routes of administration and therapeutic applications. Nano-delivery systems seem as promising solutions to these issues. They can be used for targeted-drug release, diagnostic imaging and therapy monitoring. Nanosystems allow the formulation of drug delivery systems with tailored properties (e.g. solubility, biodegradability, release kinetics and distribution) that provide means to improve cancer patients? quality of life by lowering the administered dose and, incidentally, the cost of clinical treatments. This article overviews the main features of different nanovehicles (linear and non-linear polymeric nanosystems, lipid-based systems, inorganic nanoparticles) and presents a selection of reports on applications of such systems to cancer therapy published between 2010 and 2013.","palabras_clave":"anticancer drug therapy|dendrimers|inorganic nanoparticles|liposomes|nanocapsules|nanogels|nanospheres"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62994,"idioma_id":4,"titulo":"Modeling transition diffusive - non diffusive transport in a turbid media and application to time-resolved reflectance","resumen":"In this work a generalized solution for the photon density is proposed and applied to two types of experiments in turbid media carried out in the last years. Both involve small typical distances, where it is known that the diffusion approximation ceases to be valid. In one case, the use of time-resolved reflectance at small or null source-detector separation using fast single photon gating to localize small inhomogeneities embedded in diffusive media has been proposed. In other type of experiments, it is addressed the transition between the ballistic and the diffusive regimes, measuring the transmitted light within a relatively narrow solid angle. The model proposed here corroborates the importance of the solid angle of the measurement device in order to see the ballistic photons, and the given generalized solution provides valid answers to problems posed by the mentioned experiments. Furthermore, it permits the description of diffusive photons when the absorption coefficient is relatively high, where the diffusion approximation is not valid.","palabras_clave":"Turbid Media|Ballistic Photons|Snake photons|Biomedical optics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62995,"idioma_id":4,"titulo":"Petrology, geochemistry and mineralization of the Las Aguilas and Virorco mafic-ultramafic bodies, San Luis Province, Argentina","resumen":"The layered mafic?ultramafic rocks in the Sierras de San Luis, Argentina (Las Águilas, Las Higueras and Virorco), constitute a 3?5-km-wide belt that extends over 100 km from NE to SW. They carry a sulphide mineralization consisting of pyrrhotite, pentlandite and chalcopyrite, in veins and as disseminated to massive ore. Disseminated spinels are frequently associated with the sulphide minerals as well as platinum group minerals. A strong correlation between S, Ni, Co, Cu, Cr, Pt and Pd indicates the presence of one to three levels of mineralization within the ultramafic units. The maximum concentration of these elements coincides with the units containing platinum group minerals (PGM) and spinel group minerals. This clear relationship constitutes a good prospecting guide in the search for layers with high-grade ore, probably associated with deeper stratigraphic levels where ultramafic rocks are dominant. The bulk rock chemistry and concentrations of metals and platinum group elements as well as textural evidence suggest that the parental magma was mafic with tholeiitic affinities and MgO rich. The Las Águilas layered mafic?ultramafic body and the remaining bodies in the area bear similar characteristics to well-known stratified complexes developed in extensional tectonic regimes, as it is the case of Jinchuan (China), Kabanga (Tanzania) and Fiambala (Argentina).","palabras_clave":"Layered|Mafic-ultramafic|Geochemistry|PGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62996,"idioma_id":4,"titulo":"ASR1 mediates glucose-hormone crosstalk by affecting sugar trafficking in tobacco plants","resumen":"Asr (for ABA, stress, ripening) genes are exclusively found in the genomes of higher plants and the encoded proteins have been found localized both to the nucleus and cytoplasm. However, before the mechanisms underlying the activity of ASR proteins can be determined, the role of these proteins in planta should be deciphered. Results from the present study suggest that ASR is positioned within the signaling cascade of interactions among glucose, abscisic acid and gibberellins. Nicotiana tabacum transgenic lines with reduced levels of ASR protein showed impaired glucose metabolism and altered abscisic acid and gibberellin levels. These changes were associated with dwarfism, reduced CO2 assimilation and accelerated leaf senescence as a consequence of a fine regulation exerted by ASR to the glucose metabolism. This regulation resulted in an impact on glucose signaling mediated by hexokinase1 and SnRk1 (for Snf1-related kinase) which would subsequently have been responsible for photosynthesis, leaf senescence and hormone level alterations. It thus can be postulated that ASR is not only involved in the control of hexose uptake in heterotrophic organs, as we have previously reported, but also in the control of carbon fixation by the leaves mediated by a similar mechanism.","palabras_clave":"tomato|ASR|CHO metabolism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62997,"idioma_id":4,"titulo":"Biomarkers of sensitivity to potent and selective antitumor 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole (5F203) in ovarian cancer","resumen":"2-(4-Amino-3-methylphenyl)-5-fluorobenzothiazole (5F203, NSC 703786) lysylamide belongs to a novel mechanistic class of antitumor agents. It elicits activity against ovarian, breast, kidney and colorectal cancer models. In sensitive breast cancer cells, 5F203 activates aryl hydrocarbon receptor (AhR) signaling. Herein, we evaluate the role of AhR in 5F203 activity in two ovarian cancer cell lines: IGROV-1 (sensitive to 5F203), SKOV-3 (resistant to this agent). In addition, cancer cells have been isolated from ascites fluid of ovarian cancer patients; sensitivity to 5F203 and concurrent AhR signal transduction has been examined in ascites-isolated ovarian cancer patients´ cells. 5F203 induced enhanced CYP1A1 expression, AhR translocation and ROS formation in IGROV-1 cells and ascites-isolated ovarian cancer cells that were sensitive to 5F203. In IGROV-1 cells 5F203-induced ROS formation was accompanied by JNK, ERK and P38MAPK phosphorylation, DNA damage and cell cycle arrest prior to apoptosis. In contrast, 5F203 failed to induce CYP1A1 expression, AhR translocation or oxidative stress in 5F203-resistant SKOV-3 cells, or in ovarian cancer ascites cells inherently resistant to this agent. We propose that AhR may represent a new molecular target in the treatment of ovarian tumors and 5F203 may exemplify a potential novel treatment. Furthermore, putative biomarkers of sensitivity to this agent have been identified.","palabras_clave":"ARYL HYDROCARBON RECEPTOR|5F 203|OVARIAN CANCER|BIOMARKERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62998,"idioma_id":4,"titulo":"Electrochemical method for Ag-PEG nanoparticles synthesis","resumen":"In this work we present an electrochemical method to successfully prepare silver nanoparticles using only polyethylene glycol as stabilizer and without any other reactive. Here we study the use of the polymeric stabilizer to allow the introduction of a potential tool to reinforce the control of the size and shape of the nanoparticles throughout the synthesis process. The evolution of the reactions was followed by UV-Vis spectroscopy. The electrode processes were characterized by cyclic voltammetric measurements and the final product was studied by Atomic Force Microscopy, Transmission Electron Microscopy, and X-Ray Diffraction. The influences of the current density, polymer length, and concentration media were analyzed.","palabras_clave":"Nanoparticles|Ag-PEG|Synthesis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":62999,"idioma_id":5,"titulo":"CAROTENISMO EN UN EJEMPLAR DE CHURRINCHE (Pyrocephalus rubinus)","resumen":"          Las aberraciones cromáticas son  relativamente comunes en aves, siendo las más frecuentes amelanismo,  hipomelanismo e hipermelanismo. En las aves existen, además de las melaninas,  otros pigmentos denominados carotenoides, los cuales son responsables de los  colores amarillos, naranjas y rojos del plumaje. Los carotenoides no pueden ser  sintetizados de novo y deben ser obtenidos de los alimentos al momento  de la muda. La aberración cromática relacionada con la reducción o la completa  ausencia de carotenoides, o bien con su anormal distribución en el plumaje, se  denomina carotenismo y resulta raramente descripto entre las aves silvestres.  En esta comunicación describimos un caso de carotenismo en una especie de la  familia Tyrannidae, el Churrinche (Pyrocephalus rubinus). El ejemplar  caroténico motivo de la presente comunicación representa un caso de carotenismo  inusual no sólo para el Churrinche, sino para Passeriformes neotropicales en  general.    ","palabras_clave":"CAROTENISMO|PYROCEPHALUS RUBINUS|CAROTENOIDES|OTAMENDI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63000,"idioma_id":4,"titulo":"Habitat distribution modeling reveals vegetation flammability and land use as drivers of wildfire in SW Patagonia","resumen":"Despite important recent advances in modeling current and future global fire activity in relation to biophysical predictors there remain important uncertainties about finer-scale spatial heterogeneity of fire and especially about human influences which are typically assessed at coarse-spatial resolutions. The purpose of the current study is to quantify the influence of biophysical and anthropogenic variables on the spatial distribution of wildfire activity between 1984 and 2010 over an extensive southern Patagonian-Andean region from ca. 43° to 53° S extending from coastal rainforests to xeric woodland and steppe.  We used satellite imagery to map all detectable fires > 5 ha from 1984 to 2010 in four study areas (each of 13,100 to 36,635 km2) and field checked 65 of these burns for accuracy of burned vegetation class and fire perimeters.  Then, we used the MaxEnt modeling technique to assess the relationships of wildfire distributions to biophysical and human environmental variables in each of the four regions.  The 232 fires > 5 ha mapped in the four study areas accounted for an area of 1,314 km2 indicating that at least 1.8% of the total area burned between 1984 and 2010.  In general, areas with intermediate productivity levels (e.g. shrublands) have higher fire probability compared with areas of low and high productivity levels, such as steppe and wet forests, respectively.  There is a marked contrast in the flammability of broad vegetation classes in determining fire activity at a regional scale, as well as a strong spatial relationship of wildfires to anthropogenic variables.  The juxtaposition of fire-resistant tall forests with fire-prone shrublands and woodlands creates the potential for positive feedbacks from human-set fires to gradually increase the flammability of extensive landscapes through repeated burning.  Distance to roads and settlements were also strong predictors, suggesting that fire in all regions is ignition-limited.  However, these anthropogenic predictors influenced probability of fire differently among study regions depending on their main land-use practices and their past and present socioeconomic contexts.","palabras_clave":"HUMAN-IGNITION|MAX-ENT|NOTHOFAGUS|WILDFIRE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63001,"idioma_id":4,"titulo":"Regulation of Bacillus subtilis DesK thermosensor by lipids","resumen":"Temperature sensing is essential for the survival of living cells.  The membrane-bound thermosensor DesK from Bacillus subtilis  is a key representative of histidine kinases receptors able to  remodel membrane lipid composition when the temperature  drops below &#8764;30&#9702;C. Although the receptor is well studied, a  central issue remains: how does the compositional and functional  diversity of the surrounding membrane modulate receptor  function? Reconstituting full-length DesK into proteoliposomes  of well-defined and controlled lipid composition represents  a minimal synthetic approach to systematically address this  question. Thus DesK has been reconstituted in a variety of  phospholipid bilayers and its temperature-regulated autokinase  activity determined as function of fatty acyl chain length, lipid  head-group structure and phase preference. We show that the  head group structure of lipids (both in vitro and in vivo) has little  effect on DesK thermosensing, whereas properties determined  by the acyl chain of lipids, such as membrane thickness and  phase separation into coexisting lipid domains, exert a profound  regulatory effect on kinase domain activation at low temperatures.  These experiments suggest that the non-polar domain of  glycerolipids is essential to regulate the allosteric structural  transitions of DesK, by activating the autophosphorylation of  the intracellular kinase domain in response to a decrease in  temperature.","palabras_clave":"Desk|quinasa|lipidos |enzima"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63002,"idioma_id":4,"titulo":"Zr-supported CuFe2O4 nanoparticles as catalysts for the combustion of propane","resumen":"The nanoparticles of CuFe2O4, with spinel structure, supported over ZrO2   and sulphated ZrO2, were obtained by the precursor citrate route. The   supported catalysts, containing a ferrite\/support ratio of 30%, were   thermally treated within the range 150 -700°C, characterized by XRD,   TPR, FTIR, SEM-EDX, TEM and Mössbauer spectroscopy. The propane   combustion was performed as a reaction test and the influence of the   thermal treatment was analyzed. Despite the differences in the   physico-chemical characteristics of the Zr supports, both supported   CuFe2O4 catalysts, containing 30% loading and treated at 400°C, behave   as active catalysts for propane combustion of similar efficiency.   Furthermore, catalytic activity decreases upon heating, mainly due to   crystal growth and, in ZS supported samples due to a change in the   structure of the support caused by desulphation at around 600°C. The   effect of surface available cations is discussed according to Mossbauer   spectra.","palabras_clave":"Copper ferites|supported CuFe2O4 |Catalyst activity|propane combustion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63003,"idioma_id":4,"titulo":"Dynamical behavior of martensite-austenite transitions: simulations and experiments","resumen":"The dynamical behavior of the reverse martensitic transformation has been numerically simulated with an atomistic model and compared with experiments in Cu-Zn-Al alloys. Starting from different configurations of the martensitic variants (varying mainly their mean size), the transformation to austenite was studied as a function of the heating speed. Both, experimental and numerical results show that at low velocities there is no dependence of the transition temperatures, whereas at higher speeds they gradually increase. Simulations allow us to have an insight of the underlying processes during the transition to austenite. They also show that a heating speed independent transition can only be obtained when a microstructure of very small variants is present.","palabras_clave":"Solid-solid phase transitions|Martensitic transformations|Numerical simulations|Atomistic model|Cu-Zn-Al alloys|Mechanical experiments|Heating rate|Variant configuration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63004,"idioma_id":4,"titulo":"Characterization and Comparative Study of Pseudo-Elastic Cu-Zn-Al foams synthesized by two different methods","resumen":"Low cost Cu-Zn-Al foams present, in specific composition ranges, good   pseudoelastic properties associated with interesting damping capacities,   which establish these materials as attractive from the point of view of   structural applications. These foams are manufactured by infiltration   of small SiO2 spheres into the molten alloys. After alloy solidification the SiO2   spheres are removed by immersing the material in a solution of aqueous   hydrofluoric acid. In this work, we have investigated the effect of two   different foam preparation techniques: inductive and resistive melting.   The paper details both the physic-chemical and mechanical   characterization of the foams synthesized with the two different   methods. The purpose of this study is to determine the quality of each   synthesis method in order to identify the best alternative.","palabras_clave":"ALLOY MELTING|MARTENSITIC TRANSFORMATION|POROUS MATERIAL|SHAPE MEMORY ALLOY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63005,"idioma_id":5,"titulo":"?Mecanismo de acción de la toxina alfa-hemolisina de Escherichia coli","resumen":"Escherichia coli es una de las bacterias anaerobias facultativas más predominantes en el intestino, siendo, en la mayoría de los casos, inocua para el huésped. Existen cepas que traslocan al torrente sanguíneo causando enfermedades extraintestinales como infecciones urinarias, septicemia y meningitis. Dentro de éstas se encuentran las cepas uropatogénicas (Uropathogenic Escherichia coli: UPEC), que secretan varios factores de virulencia. Estos últimos incluyen: toxinas, sistemas de adquisición de hierro, adhesinas y antígenos capsulares. Las principales toxinas secretadas son: alfa-hemolisina (HlyA) y el factor necrotizante citotóxico 1 (CNF-1). En esta revisión se presenta una descripción exhaustiva de HlyA, incluyendo su síntesis, maduración y exportación desde la bacteria. La acilación de la proteína en dos residuos internos de lisina la convierte en una toxina muy virulenta al exponer regiones intrínsecamente desordenadas que son esenciales en diferentes pasos del mecanismo de acción de la misma. Específicamente, la exposición de estas regiones está involucrada en interacciones proteína-proteína dentro del proceso de oligomerización. La formación del oligómero es responsable de la permeabilidad inducida en las células blanco. Finalmente, basado en los conocimientos acerca de las características estructurales y funcionales de HlyA, se presentan potenciales usos de HlyA en terapias basadas en toxinas.","palabras_clave":"TOXINAS|OLIGOMERIZACIÓN|MICRODOMINIOS DE MEMBRANA|INMUNOTOXINAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63006,"idioma_id":4,"titulo":"New at-sea records of pelagic seabirds in the South Atlantic Ocean and Antarctica","resumen":"In   austral summer 1994-95, 2009, 2010, 2011 and 2012 during a ship-based   seabird survey in the south Atlantic and Antarctica we documented at-sea   distributions of Buller´s albatross (Thalassarche bulleri), Atlantic   petrel (Pterodroma incerta), Softplumaged petrel (P. mollis), Kerguelen   petrel (Lugensa brevirostris), and Great-winged petrel (P. macroptera).   In some cases, sightings were considered as extralimital but for other   species, their distributions need updating. Atlantic Petrel, for   example, has been regularly observed in the Drake Passage and north of   the Antarctic Peninsula for about 30 years, but the species distribution   has not been updated in the literature. Our results will contribute to   update the at sea species` distributions and their changes.","palabras_clave":"SEABIRDS|ANTARCTICA|SOUTH ATLANTIC OCEAN|DISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63007,"idioma_id":4,"titulo":"Image texture analysis and gas sensor array studies applied to vanilla encapsulation by octenyl succinic anhydride starches","resumen":"Native starch derivatization with octenyl succinic anhydride (OSA) is a chemical modification designed to enhance flavor microencapsulation performance. Hi Cap 100 and Capsul are two OSA starches derived from waxy maize base, which are especially suited for encapsulation processes. This work performs for the first time the encapsulation of vanilla extract with Capsul and Hi Cap 100 using both spray and freeze drying procedures. The encapsulation efficiency was studied correlating the starch texture with the aroma retention. Texture analysis was accomplished by means of grey level co-occurrence matrix feature extraction (GLCM), yielding image parameters that clearly differ in function of the type of starch and the drying method used for the encapsulation of the flavor. In parallel, the data recorded with a gas sensor array (e-nose) and analyzed by unsupervised multivariate methods allowed to follow up the evolution of the aroma through the whole process. The joint analysis of the GLCM and sensor array recorded data indicates that Capsul shows a higher capacity for vanilla encapsulation than Hi Cap 100. In addition, the obtained converging information from GLCM and e-nose data clearly indicates that particle texture and aroma encapsulation are connected.","palabras_clave":"Gas sensor array|OSA starches|spray and freeze drying, vanilla, |vainilla"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63008,"idioma_id":4,"titulo":"DNA  sequence  variation  of  drought-response  candidate  genes  in  Austrocedrus chilensis","resumen":"Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress.Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (&#960; = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection.Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.","palabras_clave":"ADAPTIVE VARIATION|BALANCING SELECTION|PATAGONIAN CYPRESS|SINGLE NUCLEOTIDE POLYMORPHISM (SNPs)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63009,"idioma_id":4,"titulo":"Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite","resumen":"The main objective of this study was to evaluate the interference of environment components on the in vitro evaluation of  aflatoxin B1 adsorption capacity of sodium bentonite under simulated gastrointestinal conditions of monogastric and ruminant  animals. Sodium bentonite showed a high aflatoxin B1 affinity with all of the assays. Langmuir or sigmoid isotherms were  found in different assays. Both the affinities and the surface excesses at monolayer saturation were affected by the buffer  components. The specific influence of ions in each buffer solution was investigated. A significant decrease in the surface  excess at monolayer saturation was observed under ionic strength control. A change in the isotherm shape from sigmoidal to  Langmuir was observed with the increase in the sodium chloride concentration. This was attributed to the decrease in the  importance of lateral interaction between adsorbed toxin molecules compared with surface-molecules interactions under a high  salt coverage. The presence of rumen fluid components in the adsorption environment decreased the aflatoxin B1 maximum  adsorption capacity of sodium bentonite. Despite the high affinity of this adsorbent to capture aflatoxin B1, different substances  present in the environment could affect the adsorption capacity, at least at low toxin concentrations that mimic chronic  exposure. The environment of the gastrointestinal tract, in either monogastric or ruminant animals, affect in vivo aflatoxin B1  adsorption by sodium bentonite and should be taken into account when an in vitro performance evaluation is done.","palabras_clave":"SODIUM BENTONITE|HPLC|AFLATOXIN B1|ADSORPTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63010,"idioma_id":4,"titulo":"Fungi and mycotoxins in silage: an overview","resumen":"The present revision shows the early and current knowledge in the field of silage fungi and mycotoxins explaining the relevance of fungi and mycotoxins in silage. The problem does not end in animal disease or production losses since mycotoxins in feed can lead to the presence of their metabolic products in dairy products which will be eventually affecting human health, mainly infants. Silage is green forage preserved by lactic fermentation under anaerobic conditions. This ecosystem maintains its quality and nutritional value depending on interactions among physical, chemical and biological agents. Forages used for ensilage are naturally in contact with yeasts and filamentous fungi and the contamination often occurs in the field and can also occur during harvesting, transport, storage. Moreover, post harvest poor management can lead to a rapid spoilage. Studies on fungal contamination of dairy cattle feed have shown how corn silage influences the contamination degree of feed supplied to livestock. Increasing knowledge in this area will help elucidate the influence that this microbiota exerts on production and\/or degradation of mycotoxins present in silage. Some of these fungi, although opportunist pathogens, are relevant epidemiologically; and represent a high risk of contamination to farm workers who handle them improperly.","palabras_clave":"fungi|mycotoxins|silage"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63011,"idioma_id":4,"titulo":"Evaluation of the Hg2+ binding potential of fulvic acids from Fluorescence Excitation-Emission Matrices.","resumen":"The effect of Hg2+ on the fluorescence intensity of three fulvic acids (Pahokee Peat, Pony Lake andSuwannee River) was studied. The fluorescence intensity decreased in the presence of added Hg2+, whilethe fluorescence lifetimes were independent of the concentration of Hg2+ in solution. These results areindicative of ground-states association between the fulvic acids and Hg2+ with formation of stable nonfluorescentcomplexes (static quenching process). The analysis of the excitation?emission matrices withthe Singular Value Decomposition (SVD) and Multivariate Curve Resolution ? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?2+ on the fluorescence intensity of three fulvic acids (Pahokee Peat, Pony Lake andSuwannee River) was studied. The fluorescence intensity decreased in the presence of added Hg2+, whilethe fluorescence lifetimes were independent of the concentration of Hg2+ in solution. These results areindicative of ground-states association between the fulvic acids and Hg2+ with formation of stable nonfluorescentcomplexes (static quenching process). The analysis of the excitation?emission matrices withthe Singular Value Decomposition (SVD) and Multivariate Curve Resolution ? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?fluorescence intensity decreased in the presence of added Hg2+, whilethe fluorescence lifetimes were independent of the concentration of Hg2+ in solution. These results areindicative of ground-states association between the fulvic acids and Hg2+ with formation of stable nonfluorescentcomplexes (static quenching process). The analysis of the excitation?emission matrices withthe Singular Value Decomposition (SVD) and Multivariate Curve Resolution ? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?fluorescence lifetimes were independent of the concentration of Hg2+ in solution. These results areindicative of ground-states association between the fulvic acids and Hg2+ with formation of stable nonfluorescentcomplexes (static quenching process). The analysis of the excitation?emission matrices withthe Singular Value Decomposition (SVD) and Multivariate Curve Resolution ? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?2+ with formation of stable nonfluorescentcomplexes (static quenching process). The analysis of the excitation?emission matrices withthe Singular Value Decomposition (SVD) and Multivariate Curve Resolution ? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern??emission matrices withthe Singular Value Decomposition (SVD) and Multivariate Curve Resolution ? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?? Alternating Least Squares(MCR-ALS) methods provided additional valuable information regarding the binding properties betweenHg2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?2+ ions and specific fluorescence components of the fulvic acids. The three fulvic acids were shown tocontain the same three groups of fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern?fluorophores characterized by excitation\/emission pairs in the followingregions: (320?330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern??330 nm\/425?450 nm), (370?375 nm\/465?500 nm), (290?295 nm\/370?395 nm). Thesepairs are almost not affected by the change of pH from 2.0 to 7.0. Ryan?Weber and modified Stern??Weber and modified Stern?Volmer methods were used to analyze the static fluorescence quenching of the individual components.Similar conditional stability constants of Hg2+ binding for the three components were found by bothmethods. The obtained log K values are in the range of 4.4 to 5.4.fluorescence quenching of the individual components.Similar conditional stability constants of Hg2+ binding for the three components were found by bothmethods. The obtained log K values are in the range of 4.4 to 5.4.2+ binding for the three components were found by bothmethods. The obtained log K values are in the range of 4.4 to 5.4.K values are in the range of 4.4 to 5.4.","palabras_clave":"Fulvic acids|Excitation-Emission Matrices|MCR-ALS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63012,"idioma_id":4,"titulo":"Nitrobenzene degradation in Fenton-like systems using Cu(II) as catalyst. Comparison between Cu(II)- and Fe(III)-based systems","resumen":"Nitrobenzene (NBE) degradation in Fenton-like systems using copper(II) as catalyst wasinvestigated and compared with the trends observed in iron(III)-based systems. UV-visspectroscopy, HPLC and TOC measurements were used to characterize the evolution ofthe reaction mixtures. Kinetic profiles were analyzed under a wide range of experimentalconditions in order to assess the effects of the reaction temperature, initial concentrationsand working pH. For both Cu(II)-based and Fe(III)-based systems, treatment timesdecreased with increasing oxidant and catalyst concentrations, but augmented withincreasing NBE concentration. However, significant differences were observed for Cu(II)-based systems: higher activation energy above room temperature, broader pH range ofactivity with an optimal pH of about 6.2, absence of 1,3-dinitrobenzene among the reactionproducts and important reductions in the TOC levels even in the absence of irradiation.","palabras_clave":"NITROBENCENO|FENTON|CATALIZADORES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63013,"idioma_id":4,"titulo":"The Oxidative Stress Induced in Vivo by Shiga Toxin -2 Contributes to The Pathogenicity of Hemolytic Uremic Syndrome","resumen":"Typical haemolytic uraemic syndrome (HUS) is caused by Shiga toxin (Stx)-producing Escherichia coli infections and is characterized by thrombotic microangiopathy that leads to haemolytic anaemia, thrombocytopenia and acute renal failure. Renal or neurological sequelae are consequences of irreversible tissue damage during the acute phase. Stx toxicity and the acute inflammatory response raised by the host determine the development of HUS. At present there is no specific therapy to control Stx damage. The pathogenic role of reactive oxygen species (ROS) on endothelial injury has been largely documented. In this study, we investigated the in-vivo effects of Stx on the oxidative balance and its contribution to the development of HUS in mice. In addition, we analysed the effect of anti-oxidant agents as therapeutic tools to counteract Stx toxicity. We demonstrated that Stx induced an oxidative imbalance, evidenced by renal glutathione depletion and increased lipid membrane peroxidation. The increased ROS production by neutrophils may be one of the major sources of oxidative stress during Stx intoxication. All these parameters were ameliorated by anti-oxidants reducing platelet activation, renal damage and increasing survival. To conclude, Stx generates a pro-oxidative state that contributes to kidney failure, and exogenous anti-oxidants could be beneficial to counteract this pathogenic pathway.","palabras_clave":"Shiga toxin 2 |oxidative stress-|Hemolytic Uremic Síndrome"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63014,"idioma_id":4,"titulo":"2,3,5-Trimethylphenol oxidation over Co-based solid catalysts","resumen":"2,3,5-Trimethylphenol (TMP) was oxidized with hydrogen peroxide employing cobalt based solid materials. K6[CoW12O40]·6H2O (CoW12) was probed as selective catalyst for the oxidation obtaining better results when it was supported over ZSM-5 zeolites (CoW12-ZSM-5). CoW12-ZSM-5 was found to be an ef&#64257;cient catalyst in the oxidation of TMP to 2,3,5-trimethylbenzoquinone (TMBQ) and 2,3,5-trimethylhydroquinone (TMHQ) undermild reaction conditions. Liquid phase reaction parameters were evaluated obtaining better results employing hydrogen peroxide as oxidant, with an oxidant\/substrate molar ratio of 5, methanol as solvent and 60&#9702;C as reaction temperature. An apparent activation energy of 94.19kJ\/mol was obtained.","palabras_clave":"2,3,5- Trimethylhydroquinone|2,3,5-Trimethylphenol|Co-based solid catalysts|Hydrogen peroxide"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63015,"idioma_id":4,"titulo":"Resistant estimates for high dimensional and functional data based on random projections","resumen":"We herein propose a new robust estimation method based on random projections that is adaptive and, automatically produces a robust estimate, while enabling easy computations for high or infinite dimensional data. Under some restricted contamination models, the procedure is robust and attains full efficiency. We tested the method using both simulated and real data.","palabras_clave":"robust estimates|high dimensional data|trimming procedures"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63016,"idioma_id":4,"titulo":"Interpretable clustering using unsupervised binary trees","resumen":"We herein introduce a new method of interpretable clustering that uses  unsupervised binary trees. It is a three-stage procedure, the first stage of which entails  a series of recursive binary splits to reduce the heterogeneity of the data within the  new subsamples. During the second stage (pruning), consideration is given to whether  adjacent nodes can be aggregated. Finally, during the third stage (joining), similar  clusters are joined together, even if they do not share the same parent originally.  Consistency results are obtained, and the procedure is used on simulated and real data  sets.","palabras_clave":"UNSUPERVISED CLASSIFICATION|CART|PATTERN RECOGNITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63017,"idioma_id":4,"titulo":"Effect of coiled-coil peptides on the function of the type III secretion system-dependent activity of enterohemorragic Escherichia coli O157:H7 and Citrobacter rodentium","resumen":"Many animal and human pathogenic Gram-negative bacteria such as Salmonella, Yersinia,Enterohemorrhagic Escherichia coli (EHEC) and Enteropathogenic Escherichia coli (EPEC) possess a type III secretion system (TTSS) that is used to deliver virulence proteins directly into the host cell. Recent evidence suggested that CoilA and CoilB, two synthetic peptides corresponding to coiled-coil domains of the translocator protein EspA, were effective in inhibiting the action of TTSS from enteropathogenic E. coli (EPEC). In the current study, the action of these coiled-coil peptides on the TTSS of EHEC O157:H7 and C. rodentium was examined. CoilA and CoilB showed to be effective in reducing the red blood cell (RBC) lysis mediated by EHEC O157:H7 and the in vitro secretion of translocator proteins EspB and EspD by EHEC O157:H7 and EspD by C. rodentium. Treatment of mice with CoilA and CoilB peptides prevented colon damage when the animals were inoculated with C. rodentium. Colon samples of the non-treated group showed areas with loss of superficial epithelium, damaged cells and endoluminal mononuclear inflammatory infiltrate, consistent with histological lesion induced by C. rodentium, whereas mice treated with the synthetic peptides kept normal the surface epithelium showing a similar structure as the uninfected control group. These encouraging results prompted us to test coiled-coil peptides as treatment or vaccines in other models of bacterial infections.","palabras_clave":"EHEC|Citrobacter|peptidos|antimicrobianos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63018,"idioma_id":4,"titulo":"Coxiella burnetii in ticks, Argentina","resumen":"We found 41 (44.6%) of 92 ticks and 2 (15.4%) of 13 of the A. tigrinum and A. parvum adult ticks, respectively, to be infected by C. burnetii. Because these ticks were collected as engorged&nbsp; nymphs from wild rodents in a natural biome of Argentina, namely, the Chaco phytogeographic domain (5,6), our results indicate that C. burnetii is established in this part of the country where ticks possibly play an essential role in the enzootic cycle. Serologic evidence of C. burnetii infection ha been found among goats and cattl in several areas of Argentina (3,4). Because free-ranging domestic cattle and goats are considered among the most likely hosts for A. parvum adult icks in the Chaco domain (6), humans are likely being exposed to C. burneti as well. ","palabras_clave":"Argentina|Coxiella|Coxiella burnetii|Research|Ticks"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63019,"idioma_id":4,"titulo":"Educating to Build Bridges","resumen":"&nbsp;In March 2012, 30 faculty and 49 studentsfrom all over the world met in El Calafate, Argentina, duringtwo intense weeks. It was the second Latin American Schoolfor Education, Cognitive, and Neural Sciences (LASchool),sponsored by the James S. McDonnell Foundation. The LASchool seeks to critically examine research findings potentiallyrelevant to the development, design, and implementation ofeffective educational practices, and to foster a new generationof researchers able to operate at the interface betweeneducation and science. Here we introduce a special issuewhich brings together theory, experiments, and educationalinterventions which emerged from ideas presented anddiscussed during the 2012 LASchool.","palabras_clave":"Education|Neuroscience"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63020,"idioma_id":4,"titulo":"Dielectric behavior of epoxy\/BaTiO3 composites using nanostructured ceramic fibers obtained by electrospinning","resumen":"Composite materials made of epoxy resin and barium titanate (BT) electrospun nanostructured fibers were prepared. BT fibers were synthesized from a sol based on barium acetate, titanium isopropoxide and poly(vinyl pyrrolidone). The fibers were heat-treated at different temperatures and characterized by X-ray diffraction, scanning electron microscopy (SEM) and Raman spectroscopy. Mats of BT fibers heat-treated at 800 °C were embedded in epoxy resin into suitable moulds. The composites were characterized by SEM and dielectric measurements were performed by means of dielectric spectroscopy. The dielectric permittivity and dielectric modulus of epoxy resin\/BT-fiber composites were measured for two types of samples: with the electrodes parallel and perpendicular to the BT fiber layers. Interestingly, composite samples with electrodes perpendicular to the fibers layers and a BT content as low as 2 vol.% led to dielectric permittivities 3-times higher than that of pure epoxy resin.","palabras_clave":"BaTiO3|Composites|Epoxy resin|Dielectric permittivity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63021,"idioma_id":4,"titulo":"Differential effect of hyperglycaemia on the immune response in an experimental model of diabetes in BALB\/cByJ and C57Bl\/6J mice: participation of oxidative stress","resumen":"Diabetes is associated with an increased risk of death from infectious disease. Hyperglycaemia has been identified as the main factor contributing to the development of diseases associated with diabetes mellitus. However, experimental evidence indicates individual susceptibility to develop complications of diabetes. In this context, the aim of this work was to study the immune response in a streptozotocin-induced type 1 diabetes in two mouse strains: BALB\/cByJ and C57Bl\/6J. The participation of hyperglycaemia and oxidative stress was also analysed. Diabetic BALB\/cByJ mice showed a decrease in both the in-vivo and in-vitro immune responses, whereas diabetic C57Bl\/6J mice had higher blood glucose but exhibited no impairment of the immune response. The influence of hyperglycaemia over the immune response was evaluated by preincubation of lymphocytes from normal mice in a high glucose-containing medium. T and B cells from BALB\/cByJ mice showed a decrease in cell viability and mitogen-stimulated proliferation and an increase in apoptosis induction. An increase in oxidative stress was implicated in this deleterious effect. These parameters were not affected in the T and B lymphocytes from C57Bl\/6J mice. In conclusion, BALB\/cByJ mice were sensitive to the deleterious effect of hyperglycaemia, while C57BL\/6J were resistant. Although an extrapolation of these results to clinical conditions must be handled with caution, these results highlight the need to contemplate the genetic background to establish models to study the deleterious effect of diabetes in order to understand phenotypical variations that are of clinical importance in the treatment of patients.","palabras_clave":"BALB\/cByJ and C57Bl\/6J mice     | diabetes| high glucose| immune response|oxidative stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63022,"idioma_id":4,"titulo":"Trimethylsilyl trichloroacetate vibrational, structural and electronic properties and their comparison with related acetates","resumen":"The molecular structure of trimethylsilyl trichloroacetate, CCl3C(O)OSi(CH3)3, was determined by ab initio (MP2) and DFT calculations using 6-31G(d), 6-311G(d, p), 6- 311++G(d, p) and 6-311++G(3df,3pd) basis sets. The infrared and Raman spectra for the liquid phase were also recorded and the bands observed assigned to the vibrational normal modes. The study was completed using natural bond orbital (NBO) analysis and atoms in molecules (AIM) calculations. The comparison between the calculated molecular geometrical parameters, conformation and vibrational properties and those measured for CX3C(O)OR [X= F, Cl and R= CH3, Si(CH3)3] was of particular interest in order to check the behavior of the C=O and C-O with respect to the different substitutions. The experimental vibrational data, along with calculated theoretical force constants, were used to define a scaled quantum mechanical force field for the target system that enabled us to estimate the measured wavenumbers with a final root-meansquare deviation of 8.92 cm-1","palabras_clave":"Ab initio calculations|DFT calculations| Infrared spectroscopy| Raman spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63023,"idioma_id":4,"titulo":"Copper and nickel catalysts supported on praseodymium-doped ceria (PDC) for the water-gas shift reaction","resumen":"The  aim  of  this  work  is  to  study  the  behavior  of  Cu  and  Ni  catalysts  supported  on  praseodymium-dopedceria  (PDC)  for  the  WGS  reaction.  Samples  were  prepared  by  impregnation  of  several  PDC  supports(praseodymium  content  in  the  range  0?50  wt%)  synthesized  by  the  urea  thermal  decomposition  method.Bare  support  and  catalysts  were  characterized  by  BET,  XRD,  oxygen  storage  capacity  and  TPR.  The  activitytests  were  carried  out  in  an  atmospheric  fixed  bed  reactor,  using  a  CO\/H2O\/H2\/N2mixture  as  the  feedstream.  Doping  of  ceria  with  small  loading  of  Pr  increases  the  surface  reducibility  and  the  oxygen  storagecapacity  of  ceria.  Nickel  catalysts  are  more  active  than  copper  ones.  In  both  cases,  catalysts  supported  onPDC  with  5  Pr  wt%  are  more  active  than  samples  supported  on  pure  ceria.  Methanation,  which  is  likelyto  occur  on  Ni  catalysts,  is  moderated  in  samples  supported  on  praseodymium-doped  ceria","palabras_clave":"CO removal|Hydrogen|Ceria support|Praseodymium|Cu-Ni catalyst"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63024,"idioma_id":4,"titulo":"Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina","resumen":"A total of 120 Burkholderia cepacia complex isolates collected during 2004\u20132010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed bycenters in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed byBurkholderia cepacia complex isolates collected during 2004\u20132010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed byBurkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.(15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.and the genetic changes occurring in infecting bacteria over the course of chronic infection.B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.","palabras_clave":"Burkholderia contaminans|Cystic|Fribrosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63025,"idioma_id":4,"titulo":"Search for single $b^*$-quark production with the ATLAS detector at $\\sqrt{s}=7$ TeV","resumen":"The results of a search for an excited bottom-quark b&#8727; in pp collisions at &#8730;s=7 TeV, using 4.7 fb&#8722;1 of data collected by the ATLAS detector at the LHC are presented. In the model studied, a single b&#8727; -quark is produced through a chromomagnetic interaction and subsequently decays to a W boson and a top quark. The search is performed in the dilepton and lepton+jets &#64257;nal states, which are combined to set limits on b&#8727;-quark couplings for a range of b&#8727;-quark masses. For a benchmark with unit size chromomagnetic and Standard Model-like electroweak b&#8727; couplings, b&#8727; quarks with masses less than 870 GeV are excluded at the 95% credibility level.","palabras_clave":"Excited quarks|New phenomena|ATLAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63026,"idioma_id":4,"titulo":"Electro-catalytic performance of 60%NiO\/Ce0.9Zr0.1O2 cermets as anodes of intermediate temperature solid oxide fuel cells","resumen":"This paper analyses the electrocatalytic performance of a 60%NiO\/Ce0.9Zr0.1O2 anode under reducing atmospheres, using a symmetric cell configuration, [anode\/electrolyte\/anode], with Samaria doped Ceria (SDC) as electrolyte. This anode exhibited excellent performance in H2 atmosphere in comparison with conventional anode materials. A dual chamber solid oxide fuel cell with 60%NiO\/Ce0.9Zr0.1O2 anode, SDC electrolyte, and Pt cathode was constructed and tested under H2 atmospheres at intermediate temperatures. Good values of power densities were obtained, indicating the good performance of 60%NiO\/Ce0.9Zr0.1O2 in the operating conditions.","palabras_clave":"CeO2eZrO2   |IT-SOFCs|ANODES|ELECTROCATALYTICAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63027,"idioma_id":4,"titulo":"Emulsifiers: Effects on Quality of Fibre-Enriched Wheat Bread","resumen":"Resistant starch (RS) is a nutritional ingredientcommonly used in bread products as dietary fibre (DF). This ingredient presents similar physiological functions than those imparted by DF, promoting beneficial effects such as the reduction of cholesterol and\/or glucose levels on blood.Quality improvement of bread containing RS, with an optimized combination of emulsifiers, will be useful in the development of new and healthy bakery products. The objective of this research was to analyse the effects of different emulsifiers on several quality parameters of dough and bread prepared with wheat flour partially substituted with resistant starch as a dietary fibre. A blend of wheat flour\/maize-resistant starch (MRS; 87.5:12.5) with sodium chloride, ascorbic acid, &#945;-amylase, compressed yeast and water was utilized. Emulsifiers were incorporated to formulations in different levels according to a simplex centroid design.The viscoelastic, textural and extensional properties of dough were analysed. Bread quality was evaluated throughout the gelatinization and retrogradation of starch, specific volume of loaves, and texture and firmness of bread crumb.The incorporation of 12.5% (w\/w) of MRS to wheat flour caused an increase of 5% in water absorption. Stabilitydecreases markedly (from 9.9 to 2.2 min) and the mixing tolerance index increased (from 79 to 35 UF). The sodium stearoyl lactylate (SSL)?diacetyl tartaric acid esters of monoglycerides (DATEM) mixture increased hardness and resistance to extension on dough, whilst dough containing Polysorbate 80 (PS80) was softer; nevertheless, both types of dough retained less CO2. An optimized concentration ofthe three emulsifiers (0.24% SSL, 0.18% PS80, 0.08% DATEM, w\/w) was obtained by surface response methodology.The bread prepared with this combination of emulsifiers presented a considerable specific volume with a very soft crumb.","palabras_clave":"RESISTANT STARCH|WHEAT FLOUR|EMULSIFIERS |BREAD MAKING QUALITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63028,"idioma_id":4,"titulo":"MISTIC: mutual information server to infer coevolution","resumen":"MISTIC (mutual information server to infer coevolution) is a web server for graphical representation of the information contained within a MSA (multiple sequence alignment) and a complete analysis tool for Mutual Information networks in protein families. The server outputs a graphical visualization of several information-related quantities using a circos representation. This provides an integrated view of the MSA in terms of (i) the mutual information (MI) between residue pairs, (ii) sequence conservation and (iii) the residue cumulative and proximity MI scores. Further, an interactive interface to explore and characterize the MI network is provided. Several tools are offered for selecting subsets of nodes from the network for visualization. Node coloring can be set to match different attributes, such as conservation, cumulative MI, proximity MI and secondary structure. Finally, a zip file containing all results can be downloaded. The server is available at http:\/\/mistic.leloir.org.ar. In summary, MISTIC allows for a comprehensive, compact, visually rich view of the information contained within an MSA in a manner unique to any other publicly available web server. In particular, the use of circos representation of MI networks and the visualization of the cumulative MI and proximity MI concepts is novel.","palabras_clave":"Coevolution|Mutual Information|Multiple Sequence Alignment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63029,"idioma_id":4,"titulo":"Vibrational, electronic and structural properties of 6-nitro- and 6-amino-2-trifluoromethylchromone: An experimental and theoretical study","resumen":"Two 2-trifluoromethylchromones, 6-nitro-2-trifluoromethylchromone (1) and 6-amino-2-trifluoromethylchromone (2) were synthesized and characterized by NMR (1H, 13C, and 19F), UV&#8722;vis, vibrational (IR and Raman) spectroscopy, MS spectrometry, and compound 1 also by single structural X-ray diffraction methods. This substance crystallizes in the monoclinic P21\/c space group with Z = 4 molecules per unit cell. In the solid, the fused rings and the amino group of 1 are coplanar and the trifluoromethyl group adopts a nearly staggered conformation. The NMR, vibrational, and electronic spectra were discussed and assigned with the assistance of DFT calculations. ","palabras_clave":"Synthesis|Spectroscopic properties|Single crystal X-ray diffraction|Quantum Chemical Calculations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63030,"idioma_id":4,"titulo":"Skull anatomy of the bizarre crocodylian Mourasuchus nativus (Alligatoridae, Caimaninae)","resumen":"          Mourasuchus is  a Miocene alligatorid genus endemic to South America,  and represented by four species. Together  with the closely related Purussaurus,  Mourasuchus is a peculiar crocodylian taxon of South American neogene  Caimaninae and one of the most bizarre forms among eusuchian crocodiles. The  phylogenetic relationships between Mourasuchus  species have not been explored and detailed  skull descriptions are scarce. The goal of this study is to provide new data on  skull morphology and cranial recesses in M. nativus, including a new tomography analysis (3D modeling). We observed that several diagnostic  characters of Purussaurus such as lack  of contact between the nasal and lacrymal bones, separation of the nasal and  frontal by the prefrontals and the posterior dorsal margin of the skull table, are  shared with Mourasuchus. M. nativus  is characterized by the presence of solid transverse squamosal eminences, large  postemporal fenestrae and a quadrate latero-caudal bridge separating V2-V3  trigeminal openings. Compared with other crocodylians, the endocast of M. nativus is similar in shape but quite  sigmoid in lateral view, the canal of the supraorbital ramus  of V2 is more vertically oriented, the thick tympanic branch canal opens in a  large foramen aligned with trigeminal foramen and the canal of the vagal (X) tympanic  ramus is also very wide. Contrary to  extant alligatorids, the median  pharyngeal recess remains paired throughout its course and only connects its opposite  fellow near the external ventral opening. The knowledge of the internal skull anatomy  of Mourasuchus contributes to the  understanding of the general morphology of alligatorids, Caimaninae, and their  variation.  ","palabras_clave":"Alligatoridae|Mourasuchus nativus|Neogene|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63031,"idioma_id":4,"titulo":"The snout of Cricosaurus araucanensis: a case study in novel anatomy of the nasal region of metriorhynchids","resumen":"Metriorhynchids are the only crocodyliforms adapted to pelagic marine life. Snout natural endocasts of the Tithonian (Late Jurassic) metriorhynchid Cricosaurus araucanensis indicated that skeletal changes defining the peculiar metriorhynchid body plan were coupled with changes of the soft cephalic anatomy such as the enlarged salt glands and restructuring of the paranasal sinus system. Seven new natural endocasts of the snout and a three-dimensional reconstruction of C. araucanensis are described. Data from these casts and the reconstruction are congruent, and they are combined into an accurate reconstruction that improves out knowledge of the preorbital anatomy. The olfactory tract, bulbs, olfactory nasal region, and the anterior extension of the antorbital sinus within the maxilla are recognized. Osteological correlates of the salt gland body are also proposed. Paleobiological inferences are erected based on the integration of natural endocasts and three-dimensional reconstruction data. It is proposed that C. araucanensis nasal salt glands were highly vascularized with a blood supply comparable to those of extant marine birds. Reduced olfactory bulbs and olfactory nasal region indicate that the aerial olfaction, differing from extant crocodilians, was not well developed.","palabras_clave":"THALATTOSUCHIA |METRIORHYNCHIDAE|PARANASAL SINUSES|OLFACTORY REGION|NATURAL VS DIGITAL ENDOCASTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63032,"idioma_id":4,"titulo":"Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant","resumen":"    The possibility that the reaction between Tl(III)  (10-75 mM)  and luminol at physiological pH requires the presence of a co-oxidant to  produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce  CL, unless the media was added with H2O2. This effect linearly  depended both on Tl(III) (10-75 mM)  and H2O2 (0.1-1 mM) concentrations. In the absence of  agitation CL kinetics followed an oscillatory pattern, resembling a Belousov-Zhabotinski  reaction. The optimal pH for the reaction was 7 with no CL detected below pH  6.5. The optimal temperature range for the reaction was 20-30 ºC, decreasing at  higher temperatures and being undetectable beyond 45 ºC. The amount of Tl(I)  recovered from samples matched the theoretical amount of Tl(OH)2+ in  samples, suggesting that this species may be the responsible for the reaction. Proposed  mechanism indicates that Tl(III) forms a complex with luminol with an apparent association  constant (K) estimated in 1.95x1010 M-1.  Superoxide anion was generated along the reaction, and the addition of  superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be  detected at micromolar concentrations at physiological pH through the reaction with  luminol, providing the presence of H2O2 as co-oxidant.  ","palabras_clave":"THALLIUM|LUMINOL|HYDROGEN PEROXIDE|CHEMILUMINESCENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63033,"idioma_id":4,"titulo":"Postnatal development of the endocrine pancreas in mice lacking functional GABAB receptors","resumen":"Adult mice lacking functional GABAB receptors (GABAB1KO) have glucose   metabolism alterations. Since GABAB receptors (GABABRs) are expressed in   progenitor cells, we evaluated islet development in GABAB1KO mice.   Postnatal day 4 (PND4) and adult, male and female, GABAB1KO, and   wild-type littermates (WT) were weighed and euthanized, and serum   insulin and glucagon was measured. Pancreatic glucagon and insulin   content were assessed, and pancreas insulin, glucagon, PCNA, and   GAD65\/67 were determined by immunohistochemistry. RNA from PND4   pancreata and adult isolated islets was obtained, and Ins1, Ins2, Gcg,   Sst, Ppy, Nes, Pdx1, and Gad1 transcription levels were determined by   quantitative PCR. The main results were as follows: 1) insulin content   was increased in PND4 GABAB1KO females and in both sexes in adult   GABAB1KOs; 2) GABAB1KO females had more clusters (&lt;500 &#956;m(2)) and   less islets than WT females; 3) cluster proliferation was decreased at   PND4 and increased in adult GABAB1KO mice; 4) increased &#946;-area at the   expense of the &#945;-cell area was present in GABAB1KO islets; 5) Ins2, Sst,   and Ppy transcription were decreased in PND4 GABAB1KO pancreata, adult   GABAB1KO female islets showed increased Ins1, Ins2, and Sst expression,   Pdx1 was increased in male and female GABAB1KO islets; and 6) GAD65\/67   was increased in adult GABAB1KO pancreata. We demonstrate that several   islet parameters are altered in GABAB1KO mice, further pinpointing the   importance of GABABRs in islet physiology. Some changes persist from   neonatal ages to adulthood (e.g., insulin content in GABAB1KO females),   whereas other features are differentially regulated according to age   (e.g., Ins2 was reduced in PND4, whereas it was upregulated in adult   GABAB1KO females).","palabras_clave":"gabab ko|endocrine pancreas|development"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63034,"idioma_id":4,"titulo":"Model of the boundary layer of a vacuum-arc magnetic filter","resumen":"A model is developed to describe the electrostatic boundary layer in a positively biased magnetic  filter in filtered arcs with low collisionality. The set of equations used includes the electron  momentum equation, with an anomalous collision term due to micro-instabilities leading to Bohm  diffusion, electron mass conservation, and Poisson equation. Analytical solutions are obtained,  valid for the regimes of interest, leading to an explicit expression to determine the electron density  current to the filter wall as a function of the potential of the filter and the ratio of electron density at  the plasma to that at the filter wall. Using a set of planar and cylindrical probes it is verified  experimentally that the mentioned ratio of electron densities remains reasonably constant for  different magnetic field values and probe bias, which allows to obtain a closed expression for the  current. Comparisons are made with the experimentally determined current collected at different  sections of a positively biased straight filter.","palabras_clave":"Boundary layer|model|vacuum arc|magnetic filter"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63035,"idioma_id":4,"titulo":"Electrical studies and plasma characterization of an atmospheric pressure plasma jet operated at low frequency","resumen":"Low-temperature, high-pressure plasma jets have an extensive use in medical and biological  applications. Much work has been devoted to study these applications while comparatively fewer  studies appear to be directed to the discharge itself. In this work, in order to better understand the  kind of electrical discharge and the plasma states existing in those devices, a study of the electrical  characteristics of a typical plasma jet, operated at atmospheric pressure, using either air or argon, is  reported. It is found that the experimentally determined electrical characteristics are consistent with  the model of a thermal arc discharge, with a highly collisional cathode sheet. The only exception is  the case of argon at the smallest electrode separation studied, around 1mm in which case the  discharge is better modeled as either a non-thermal arc or a high-pressure glow. Also, variations of  the electrical behavior at different gas flow rates are interpreted, consistently with the arc model, in  terms of the development of fluid turbulence in the external jet.","palabras_clave":"plasma jet|arc model"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63036,"idioma_id":4,"titulo":"The Circadian System: Plasticity at Many Levels","resumen":"Over the years it has become crystal clear that a variety of processes encode time-of-day information, ranging from gene expression, protein stability, or subcellular localization of key proteins, to the fine tuning of network properties and modulation of input signals, ultimately ensuring that physiology and behavior are properly synchronized to a changing environment. The purpose of this review is to put forward examples (as opposed to generate a comprehensive revision of all the available literature) in which the circadian system displays a remarkable degree of plasticity, from cell autonomous to circuit-based levels. In the literature, the term circadian plasticity has been used to refer to different concepts. The obvious one, more literally, refers to any change that follows a circadian (circa=around, diem=day) pattern, i.e. a daily change of a given parameter. The discovery of daily remodeling of neuronal structures will be referred herein as structural circadian plasticity, and represents an additional and novel phenomenon modified daily. Finally, any plasticity that has to do with a circadian parameter would represent a type of circadian plasticity; as an example, adjustments that allow organisms to adapt their daily behavior to the annual changes in photoperiod is a form of circadian plasticity at a higher organizational level, which is an emergent property of the whole circadian system. Throughout this work we will revisit these types of changes by reviewing recent literature delving around circadian control of clock outputs, from the most immediate ones within pacemaker neurons to the circadian modulation of rest-activity cycles.","palabras_clave":"circadian plasticity|circadian network|PDF|structural plasticity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63037,"idioma_id":4,"titulo":"Immune responses of poultry to Newcastle  disease virus","resumen":"Newcastle disease (ND) remains a constant threat to poultry producers worldwide, in spite of the availability and global employment of ND vaccinations since the 1950s. Strains of Newcastle disease virus (NDV) belong to the order Mononegavirales, family Paramyxoviridae, and genus Avulavirus, are contained in one serotype and are also known as avian paramyxovirus serotype-1 (APMV-1). They are pleomorphic in shape and are single-stranded, non-segmented, negative sense RNA viruses. The virus has been reported to infect most orders of birds and thus has a wide host range. Isolates are characterized by virulence in chickens and the presence of basic amino acids at the fusion protein cleavage site. Low virulent NDV typically produce subclinical disease with some morbidity, whereas virulent isolates can result in rapid, high mortality of birds. Virulent NDV are listed pathogens that require immediate notification to the Office of International Epizootics and outbreaks typically result in trade embargos. Protection against NDV is through the use of vaccines generated with low virulent NDV strains. Immunity is derived from neutralizing antibodies formed against the viral hemagglutinin and fusion glycoproteins, which are responsible for attachment and spread of the virus. However, new techniques and technologies have also allowed for more in depth analysis of the innate and cell-mediated immunity of poultry to NDV. Gene profiling experiments have led to the discovery of novel host genes modulated immediately after infection. Differences in virus virulence alter host gene response patterns have been demonstrated. Furthermore, the timing and contributions of cell-mediated immune responses appear to decrease disease and transmission potential. In view of recent reports of vaccine failure from many countries on the ability of classical NDV vaccines to stop spread of disease, renewed interest in a more complete understanding of the global immune response of poultry to NDV will be critical to developing new control strategies and intervention programs for the future.","palabras_clave":"Newcastle disease virus|Paramyxoviridae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63038,"idioma_id":4,"titulo":"Babesia bovis: lipids from virulent S2P and attenuated R1A strains trigger differential signalling and inflammatory responses in bovine macrophages","resumen":"The intra-erythrocytic protozoan&nbsp;Babesia&nbsp;bovis is an economically important pathogen that causes an acute and often fatal infection in adult cattle. Babesiosis limitation depends on the early activation of macrophages, essential cells of the host innate immunity, which can generate an inflammatory response mediated by cytokines and nitric oxide (NO). Herein, we demonstrate in bovine macrophages that lipids from B. bovis attenuated R1A strain (LA) produced a stronger NO release, an early TNFƒ¿ mRNA induction and 2-fold higher IL-12p35 mRNA levels compared to the lipids of virulent S2P strain (LV). Neither LA nor LV induced anti-inflammatory IL-10. Regarding signalling pathways, we here report that LA induced a significant phosphorylation of p38 and extracellular signal-regulated kinases 1 and 2 (ERK1\/2) whereas LV only induced a reduced activation of ERK1\/2. Besides, NF-ƒÈB was activated by LA and LV, but LA produced an early degradation of the inhibitor IƒÈB. Interestingly, LV and the majority of its lipid fractions, exerted a significant inhibition of concanavalin A-induced peripheral blood mononuclear cell proliferation with respect to LA and its corresponding lipid fractions. In addition, we determined that animals infected with R1A developed a higher increase in IgM anti-phosphatidylcholine than those inoculated with S2P. Collectively, S2P lipids generated a decreased inflammatory response contributing to the evasion of innate immunity. Moreover, since R1A lipids induced a pro-inflammatory profile, we propose these molecules as good candidates for immunoprophylactic strategies against babesiosis.","palabras_clave":"Lipids|Babesia bovis|bovine macrophages|signalling|innate immunity|anti-phospholipid antibodies"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63039,"idioma_id":4,"titulo":"Fuzzy assessment of herbicide resistance risk: Glyphosate-resistant johnsongrass, Sorghum halepense (L.) Pers, in Argentina's croplands.","resumen":"A fuzzy-logic based model was built in order to assess the relative influence of different ecological and management drivers on glyphosate resistance risk (GRR) in Sorghum halepense (L.) Pers. The model was hierarchically structured in a bottom-up manner by combining 16 input variables throughout a logical network. Input data were related to 1) herbicide usage, 2) crop rotation, 3) landscape characterization, 4) weed dispersal, and 5) mean maximum and minimum seasonal temperature. Mean maximum and minimum seasonal temperatures and the dominance of glyphosate use were the variables that showed the highest sensitivity to input changes. Application of the model at a regional scale resulted in a wide range of GRR values. The lowest range values (lower than 0 and between 0 and 0.25) were represented in 5.5% and 21.5 % of the cropping area, respectively. Intermediate GRR range (between 0.25 and 0.5) were assessed in 57.3 % of the cropping area whilst the highest GRR range values (0.5 - 0.7) were represented in only 15.6 % of the studied area. The assessment of trade-offs between different ecosystem functions through expert opinion can complement traditional analyses for predicting herbicide resistance risk based on solely the genetic aspect of the evolutionary process.","palabras_clave":"FUZZY LOGIC|HERBICIDE RESISTANCE|MODELLING|WEED SCIENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63040,"idioma_id":4,"titulo":"Dual role of immune cells in the testis Protective or pathogenic for germ cells?","resumen":"The purpose of this review is to describe how the immune cells present in the testis interact with the germinal epithelium contributing to survival or apoptosis of germ cells (GCs). Physiologically, the immunosuppressor testicular microenvironment protects GCs from immune attack, whereas in inflammatory conditions, tolerance is disrupted and immune cells and their mediators respond to GC self antigens, inducing damage of the germinal epithelium. Considering that experimental models of autoimmune orchitis have clarified the local immune mechanisms by which protection of the testis is compromised, we described the following topics in the testis of normal and orchitic rats: (1) cell adhesion molecule expression of seminiferous tubule specialized junctions and modulation of blood-testis barrier permeability by cytokines (2) phenotypic and functional characteristics of testicular dendritic cells, macrophages, effector and regulatory T cells and mast cells and (3) effects of pro-inflammatory  cytokines (TNF-alpha, IL-6 and FasL) and the nitric oxide-nitric oxide synthase system on GC apoptosis. IL-6 and FasL and the nitric oxide- nitric oxide synthase system on GC apoptosis.","palabras_clave":"IMMUNE CELLS|GERM CELL APOPTOSIS|SEMINIFEROUS TUBULES|BLOOD-TESTIS BARRER|CYTOKINES|NITRIC OXIDE|INFLAMMATION|TESTIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63041,"idioma_id":4,"titulo":"Effect of diet on the hepatopancreas structure in the red clayfish Cherax quadricarinatus","resumen":"EFECTO DE LA DIETA SOBRE LA ESTRUCTURA DEL HEPATOPÁNCREAS EN LA LANGOSTA Cherax quadricarinatus (DECAPODA)  1Sacristán H. J.; 1Franco Tadic L. M.; 2,3 Fernandez Gimenez A 1,3 López Greco L. S.  1Lab. Biología de la Reproducción y el Crecimiento de Crustáceos Decápodos. DBBE, FCEN, UBA, Argentina. 2Laboratorio de Acuicultura, UNMdP, Mar del Plata, Argentina. 3 CONICET hsacristan@bg.fcen.uba.ar  El hepatopáncreas (HP) de los crustáceos es el principal órgano de síntesis y secreción de enzimas digestivas, absorción y almacenamiento de lípidos y glucógeno. La unidad funcional es un túbulo ciego de epitelio cilíndrico simple formado por cuatro tipos celulares principales: E, F, R y B. El objetivo es evaluar el efecto de tres dietas sobre la estructura del HP de juveniles de C. quadricarinatus. La composición de las dietas utilizadas fue: 6,50%, 4,40 y 16,03 de lípidos y 47,50%, 37,45 y 3,85 de proteína para las Dietas 1 (D1), 2 (D2) y 3 (D3) respectivamente. Juveniles de peso promedio de 1, 7 y 14 gr fueron alimentados diariamente durante 24 días (5 animales por cada peso y dieta). Se disecó el HP y se procesó para microscopía óptica. Los animales alimentados con D1 no mostraron alteraciones estructurales mientras que con D2 se observó reducción en el número de células F, hipertrofia de células B, algunas áreas con menor altura del epitelio y escasa diferenciación entre las células B y R. Con la D3 se observaron los mismos tipos de alteraciones que con D2 pero mucho más acentuadas, además de la pérdida de la estructura tubular. Concluímos que la composición de la D1, al menos en cuanto al balance proteína: lípidos, es más apropiada para la alimentación de los juveniles de C. quadricarinatus. Financiación: PICT 2007 (01187), X458 (UBACYT 2008-2011), PIP 2009 (129),  Palabras claves: hepatopáncreas; C. quadricarinatus; dieta.","palabras_clave":"LANGOSTA|HISTOLOGIA|HEPATOPANCREAS|NUTRICION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63042,"idioma_id":4,"titulo":"Effect of crude oil petroleum hydrocarbons on protein expression of the prawn Macrobrachium borellii","resumen":"Hydrocarbon pollution is a major environmental threat to ecosystems in   marine and freshwater environments, but its toxicological effect on   aquatic organisms remains little studied. A proteomic approach was used   to analyze the effect of a freshwater oil spill on the prawn   Macrobrachium borellii. To this aim, proteins were extracted from midgut   gland (hepatopancreas) of male and female prawns exposed 7days to a   sublethal concentration (0.6ppm) of water-soluble fraction of crude oil   (WSF). Exposure to WSF induced responses at the protein expression   level. Two-dimensional gel electrophoresis (2-DE) revealed 10 protein   spots that were differentially expressed by WSF exposure. Seven proteins   were identified using MS\/MS and de novo sequencing. Nm23 oncoprotein,   arginine methyltransferase, fatty aldehyde dehydrogenase and glutathione   S-transferase were down-regulated, whereas two   glyceraldehyde-3-phosphate dehydrogenase isoforms and a lipocalin-like   crustacyanin (CTC) were up-regulated after WSF exposure. CTC mRNA levels   were further analyzed by quantitative real-time PCR showing an   increased expression after WSF exposure. The proteins identified are   involved in carbohydrate and amino acid metabolism, detoxification,   transport of hydrophobic molecules and cellular homeostasis among   others. These results provide evidence for better understanding the   toxic mechanisms of hydrocarbons. Moreover, some of these differentially   expressed proteins would be employed as potential novel biomarkers.","palabras_clave":"BIOMARKER|CRUSTACEAN|PROTEIN DIFFERENTIAL EXPRESSION|HYDROCARBON POLLUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63043,"idioma_id":4,"titulo":"Low irradiance pulses improve postharvest quality of spinach leaves (Spinacia Oleraceae L. cv Bison)","resumen":"            The aim of this work is to extend  and improve the postharvest life of mature spinach leaves using clean  technologies like the use of short pulses of light at low irradiance. After  harvest spinach leaves were immediately sealed in polyethylene bags in the  laboratory. These bags were placed in a dark chamber at 23 ºC under continuous dark  or with the application of light pulses (LP) consisting of 15 min each 2 - 6 h  or 7 min each 2 h for 3 d. The chosen irradiance, 30 &#956;mol-1 m-2  s-1 PPFD, corresponded to the light compensation point previously  measured in spinach plants under greenhouse conditions. After the leaves were  treated with LP for 3 d, all the samples were transferred to a chamber at 4º C under  continuous dark for another week. Senescence was triggered in leaves under continuous  dark after 3 d of storage and delayed in those receiving LP. In addition ascorbic  acid and glutathione contents were kept higher in LP-treated than in untreated  spinach. These trends were conserved after storage under continuous dark and  refrigeration for another week. When LP were applied in combination with 1-MCP the  antioxidant capacity was further improved. These results demonstrate that short  LP of low irradiance can be used to extend and to improve postharvest life of  mature spinach leaves.     ","palabras_clave":"Ascorbic acid|ethylene|glutathione|light pulses|senescence|spinach"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63044,"idioma_id":4,"titulo":"CRYSTAL STRUCTURE REFINEMENT AND VIBRATIONAL ANALYSIS OF Y[Co(CN)6].4H2O AND ITS THERMAL DECOMPOSITION PRODUCTS","resumen":"Studies on Y[Co(CN)6]·4H2O by means of thermal analysis, IR and Raman spectroscopy and X-ray powder diffraction were carried out. The complex Y[Co(CN)6]·4H2O crystallizes in the orthorhombic crystal system, space group Cmcm. Y3+ ion is eight-coordinated forming bicapped distorted trigonal prism YN6O2 and Co3+ ion is octahedrally coordinated to six CN groups. The IR spectrum shows well-resolved bands in the CN stretching region due to the presence of 13C and 15N in relative natural abundance. The Raman spectrum of the complex shows two distinct set of bands of the CN stretching vibration: one is a singlet and the other is split due to the low symmetry of the Y3+ ion compared to the pentahydrate complex. The thermal decomposition was studied in order to investigate the formation of YCoO3. The crystal structure of YCoO3 was refined by means of Rietveld analysis using PXRD data. We found that it was slightly deficient in Y3+, which is in agreement with the small amount of Y2O3 found as impurity in the sample. The formula of the mixed oxide obtained by this method of synthesis is Y0.961CoO3.","palabras_clave":"CRYSTAL STRUCTURE REFINEMENT|THERMAL DECOMPOSITION|YCoO3|RAMAN SPECTROSCOPY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63046,"idioma_id":4,"titulo":"Synthesis and Characterization of Iron Oxyhydroxide Nanowires","resumen":"A very simple method of synthesis of goethite, FeOOH, nanowire is reported. To fabricate the nanowires, an anodized alumina nanoporous template (AAO) is used. AAO has pores with an averagediameter of 60 nm. The synthesis is based on a self-combustion reaction of the chemical precursor (Fe NO saturated solution) which occurs inside the nanopores. The geometry of AAO deter-mines the morphology of the nanowires and the confinement conditions in which the heat treatment determines the composition of the nanostructure. The nanowires are characterized using scanning electron microscopy, high resolution transmission electron microscopy and magnetometry [magnetization versus appliedfield (M versus H)]. TEM analysis indicates that nanowires are composed of several FeOOH single crystals. The nanowires have a clear magnetic oriented structure.","palabras_clave":"Iron oxide|Iron oxyhydroxide|Nanowires|Structural and magnetic properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63047,"idioma_id":4,"titulo":"One-step room temperature synthesis of very small &#947;-Fe2O3 nanoparticles","resumen":"                Very small maghemite nanoparticles (~3 nm) are  obtained through a one-step synthesis at room temperature. The fast neutralization  reaction (co-precipitation) of a ferric solution (FeCl3 aqueous) in  a basic medium (NH4OH concentrated) produces an intermediate phase,  presumably two-line ferrihydrite, that in oxidizing conditions is transformed  to maghemite nanoparticles. The synthesis of maghemite, as final product of the  reaction, was characterized by High-Resolution Transmission Electron Microscopy  (HR-TEM), X-ray Absorption Fine Structure (XAFS), Mössbauer spectroscopy, and  magnetometry. The XAFS technique allowed the analysis of the crystallographic  variations into maghemite nanoparticles as a result of modification in its  surface\/volume ratio. Mössbauer spectroscopy at low temperature (4.2 K)  confirms the presence of Fe(III) in tetrahedral and octahedral interstices, in  the stoichiometry corresponding to maghemite. The specific magnetization, M vs  H (3K and 300 K, up to 7T) and temperature dependence of the magnetization (50  Oe by ZFC mode, 2 K &#8804; T &#8804; 300 K) indicate that maghemite nanoparticles of 3 nm  are in superparamagnetic state with a blocking temperature close to 36 K.  ","palabras_clave":"nanopartículas magnéticas|maghemita|ferrihidrita"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63048,"idioma_id":4,"titulo":"Synthesis, characterization and biofunctionalization of magnetic gold nanostructured particles","resumen":"Magnetic Fe3O4@Au nanocomposite, nanostructured particles were prepared bydepositing gold onto seeds of superparamagnetic iron oxide nanoparticles (SPION). Theparticles were characterized by SEM, TEM, XRD, visible and near infraredspectroscopy, and magnetic measurements. They were also functionalized and employedas enzymatic carriers for electrochemical biosensing. The nanostructured particlesshowed an urchin-like morphology, roughly spherical particles whose surface wascovered with nanometric protuberances. The average diameter of the particles could becontrolled in the 100-500 nm range by changing the concentration of surfactants(oleylamine and oleic acid) employed in the synthesis. XRD showed a preferentialgrowth of {111} facets. 3-mercaptopropionic acid was used to chemically modify thesurface of the nanostructured particles and horseradish peroxidase was immobilizedonto the gold surface. Attached enzymes showed good activity even after several cyclesof magnetic collection, drying, and redispersion.","palabras_clave":"SPION|Gold|Nanostructured Particles|Biosensing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63049,"idioma_id":4,"titulo":"IBUPROFEN-MALTODEXTRIN INTERACTION:  STUDY OF ENANTIOMERIC RECOGNITION AND COMPLEX CHARACTERIZATION","resumen":"The  interaction  between  ibuprofen  and  maltodextrins  with  different  dextrose  equivalent  was  studied  in  solution  and   solid state in order to investigate the effect on the solubility of ibuprofen and to determine their usefulness in terms of   chiral recognition. Apparent binding constants were calculated using nuclear magnetic resonance spectroscopy experi-  ments and solubility studies. The results showed an increase in the apparent solubility of ibuprofen in the presence of   maltodextrins that depended on their ionization state. The freeze-drying method was used to prepare solid complexes,   while physical  mixtures were  obtained  by simple  blending.  These solid  systems  were  characterized in  the  solid  state   using differential scanning calorimetry, thermogravimetric analysis, Fourier Transform-Infrared spectroscopy, scanning   electron microscopy and X-ray diffractometry. Detailed nuclear magnetic resonance studies provided evidence of the   influence of the type and concentration of the maltodextrin host on the chiral recognition of racemic ibuprofen, indicat-  ing that these linear ligands act as chiral selectors.","palabras_clave":"IBUPROFEN|MALTODEXTRIN|CHIRAL RECOGNITION|COMPLEX CHARACTERIZATION|NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY|SOLUBILITY STUDIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63050,"idioma_id":4,"titulo":"Spatial and temporal epidemiology of bovine trichomoniasis and bovine genital campylobacteriosis in La Pampa province (Argentina)","resumen":"The  venereal diseases bovine trichomoniasis (BT) and bovine genital  campylobacteriosis (BCG) cause economic losses in endemic areas like  La Pampa province in Argentina, where beef cattle are usually managed  extensively. This study used data compiled under a Provincial  Programme for the Control and Eradication of BT and BGC (PCE) to  determine the spatio-temporal distribution of these diseases and  identify spatial clusters.  The  study pop- ulation comprised 29,178 non-virgin bulls drawn from 3766  herds, tested for BT and BGC in 2010. Preputial smegma samples were  cultured for BT detection, while BGC was diagnosed by direct  immunofluorescence testing of these samples. Campylobacter fetus  infection  was  detected in 1.5% of bulls and 2.3% of herds, and Tritrichomonas  foetus infection was found in 1.1% of bulls and 5.1% of herds. The  proportion of positive tests was highest in February for BT, while in  April it was highest for BCG, and was inversely related to the number  of tests, which was greatest during the breeding season (spring). An  elliptical spatial cluster of high risk for BGC and a circular  cluster  for BT were  both  identified in the south of La Pampa province, which could not be  explained by cattle herd density. The spatial and temporal patterns  identified in this study provide baseline data for monitoring the  success of BT and BGC control activities in La Pampa","palabras_clave":"VENEREAL DISEASES|EPIDEMIOLOGY|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63051,"idioma_id":4,"titulo":"Microbiological culture broth designed from food waste","resumen":"The current and growing pollutions (air, water and soil) are, in part, the result of inadequate management of municipal solid waste (MSW). The relation between Public Health - storage, collection and improper disposal of solid waste, has encouraged several studies, and these results have attributed to this interrelationship the spread of over twenty human and animal diseases. The term 'single cell protein (SCP), is referred to the microbial biomass used as a dietary additive. It has high nutritional value because of its high protein content of biological quality (presence, in general, all essential amino acids), of vitamins and lipids. The aim of this work was focused on the use of nutrients contained in organic waste, conduced to design culture media for microbiological assays and, to produce single cell proteins for animal feed. The nutritional quality (percentage values) obtained after processing the food debris allowed infer a good chance for microbial growth, because carbohydrate (1.00 ± 0.03 %) and protein (0.77 ± 0.18) levels were not contained as limiting growth factors. The microorganisms considered to study the effectiveness between both culture media, Food Waste and LAPTg, were: Lactobacillus plantarum ATCC 7469, Lactobacillus rhamnosus, Enterococcus, Staphylococcus aureus, Shigella sonnei, . In all cases, the CFU\/mL obtained from  Food Waste (FW) and LAPTg culture media, showed not significant statically differences (p>0,05). In addition, to determine the behavior of Food Waste (FW) and LAPTg culture media, showed not significant statically differences (p>0,05), comparative studies involving Molasses   broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.  broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.    broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.    broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.    broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.    broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.  L1, comparative studies involving Molasses   broth (MB), FW Broth and Basal Broth (BB), were carried out. The  biomass yield calculated at 24 h, from FW Broth was 13% lower than  from the MB. The FW Broth, despite showing lower biomass yield,  statistically significant (p   molasses is not available. FW Broth can be elaborated, at low cost, in  any region of the world, because its ingredients are wastes generated by  the population. It has great versatility, allowing the development of a  wide variety of microorganisms, both Gram negative and Gram positive  bacteria as well as yeasts. The production of safe protein additives, with  high biological quality and low cost, is a current view in front of the  increasing global demand about food for human and animal.","palabras_clave":"food waste broth|single cell proteins|bacterial growth|microbial biotechnology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63052,"idioma_id":4,"titulo":"Quantum Origin of the Primordial Fluctuation Spectrum and its Statistics","resumen":"The account of the origin of cosmic structure, as provided by the standard  inflationary paradigm, is not fully satisfactory, as has been argued in Perez  et al 2006. The central point of that work is to point out the need to discuss  and explore the physical mechanism that is capable of generating the  inhomogeneity and anisotropy of our Universe, starting from an exactly  homogeneous and isotropic initial state associated with the early inflationary  regime. We review this issue briefly here together with the proposal to address  this shortcoming in terms of a dynamical collapse of the vacuum state of the  inflaton field. We also briefly indicate how this issues might be connected to  other questions being faced in the study of the quantum\/gravity interface, and  their relevance to the investigations concerning the statistical  characterization of the primordial spectrum.","palabras_clave":"Cosmic Microwave Background|Non gaussianities|statistics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63053,"idioma_id":4,"titulo":"REDISCOVERY AND LECTOTYPIFICATION OF DICRANELLA LORENTZII (DICRANELLACEAE, BRYOPHYTA)","resumen":"Suárez, G., J. Larraín & M.M. Schiavone. 2013. Rediscovery and lectotypification of Dicranella lorentzii (Dicranellaceae, Bryophyta). Boletín de la Sociedad Argentina de Botánica 48(1): 53?57","palabras_clave":"BRYOPHYTA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63054,"idioma_id":4,"titulo":"Measurement of in-plane displacements using the phase singularities generated by directional wavelet transforms of speckle pattern images","resumen":"We present a method to determine micro and nano in-plane displacements based on the phase singularities generated by application of directional wavelet transforms to speckle pattern images. The spatial distribution of the obtained phase singularities by the wavelet transform configures a network, which is characterized by two quasi-orthogonal directions. The displacement value is determined by identifying the intersection points of the network before and after the displacement produced by the tested object. The performance of this method is evaluated using simulated speckle patterns and experimental data. The proposed approach is compared with the optical vortex metrology and digital image correlation methods in terms of performance and noise robustness, and the advantages and limitations associated to each method are also discussed.","palabras_clave":"Coherence and statistical optics : Speckle|Image processing : Digital image processing|Instrumentation, measurement, and metrology : Speckle imaging|Other areas of optics : Phase"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63055,"idioma_id":4,"titulo":"Thermal Stability of Peroxy Acyl Nitrates Formed in the Oxidation of C x F 2 x +1 CH 2 C(O)H ( x = 1,6) in the Presence of NO 2","resumen":"Theformation of&nbsp; CxF2x+1CH2C(O)OONO2(x=1,6) from the photo oxidation of&nbsp; CxF2x+1CH2C(O)H(x=1,6) in the presence of NO2 was investigated. Infrared spectrumof C6F13CH2C(O)OONO2 is reportedfor the first time, and thermal stability for both peroxynitrates at 295 K and9.0 mbar are informed. Kinetic parameters (activation energy andpre-exponential factor) for CF3CH2C(O)OONO2 at9.0 and 1000 mbar are: 108 ± 2 kJ\/mol, 1.5 x 1015, and 114 ± 2kJ\/mol, 2.4 x 1016, respectively. Comparison with fluoro and hydrogenatedperoxy acyl nitrates is presented","palabras_clave":"Peroxynitrates|Telomeric alcohols|Thermal stability|Atmospheric degradation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63056,"idioma_id":4,"titulo":"Accumulation and biochemical effects of microcystin-LR on the Patagonian pejerrey (Odontesthes hatcheri) fed with the toxic cyanobacteria Microcystis aeruginosa","resumen":"We studied accumulation and biochemical effects of microcystin-LR (MCLR) in Odontesthes hatcheri after dietary administration of the cyanobacteria Microcystis aeruginosa (1.3 &#956;g MCLR \/ g body mass,incorporated in standard fish food). After 12 h, MCLR content in liver did not differ between fish fed with crushed or intact cells, demonstrating O.hatcheri?s capacity to digest cyanobacteria and absorb MCLR. In the second experiment, fish received toxic cells, non-toxic cells or control food; MCLR accumulation was monitored for 48 h. Protein phosphatase 1 (PP1), catalase (CAT), glutathione S-transferase (GST) activities and lipid peroxidation (as MDA) were measured in liver and intestine. Methanol-extractable MCLR was determined by PP1 inhibition assay (PPIA), extractable and protein-bound MCLR were measured by Lemieux  oxidation - gas chromatography \/ mass spectrometry (GC\/MS). MCLR  Accumulated rapidly up to 22.9 and 9.4 &#956;g MCLR \/ g in intestine and liver respectively followed by a decreasing tendency. Protein-bound MCLR  represented 66 to ca. 100% of total MCLR in both tissues. PP1 activity remained unchanged in intestine but was increased in liver of MCLR treated fish.CAT and GST activities and MDA content were significantly  increased by MCLR only in liver. We conclude that O. hatcheri is able to digest cyanobacteria, accumulating MCLR mostly bound to proteins. Our data suggest that this freshwater fish can be adversely affected by  cyanobacterial blooms. However, the rapid decrease of the detectable MCLR in both tissues could imply that sublethal toxin accumulation is rapidly reversed.","palabras_clave":"ODONTESTIS HACHERI|MICROCISTIN-LR|DIGESTION|OXIDATIVE STRESS|ACUMULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63057,"idioma_id":4,"titulo":"Delineating the 'galectin signature' of the tumor microenvironment.","resumen":"Galectins, a family of glycan-binding proteins, can control tumor progression by promoting transformation, angiogenesis and immune escape. We identified a dynamically regulated ?galectin signature?, which delineates the progression of prostate cancer, highlighting galectin-1 as an attractive target for anti-angiogenic therapy in advanced stages of the disease.","palabras_clave":"angiogenesis|galectins|gene signature|metastasis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63058,"idioma_id":4,"titulo":"Activation of H(+)-ATPase by glucose in Saccharomyces cerevisiae involves a membrane serine protease.","resumen":"Background: Glucose induces H+-ATPase activation in Saccharomyces  cerevisiae. Our previous study showed that (i) S. cerevisiae plasma membrane H+-ATPase forms a complex with acetylated tubulin (AcTub), resulting in inhibition of the enzyme activity; (ii) exogenous glucose addition results in the dissociation of the complex and recovery of the enzyme activity.  Methods: We used classic biochemical and molecular biology tools in order to identify the key components inth e mechanism that leads to H+-ATPase activation after glucose treatment.  Results: We demonstrate that glucose-induced dissociation of the complex is due to pH-dependent activation of a protease that hydrolyzes membrane tubulin. Biochemical analysis identified a serine protease with a kDa of  35?40 and an isoelectric point between 8 and 9. Analysis of several knockout yeast strains led to the detection of Lpx1p as the serine protease responsible of tubulin proteolysis. When lpx1Ä cells were treated with glucose, tubulin was not degraded, the AcTub\/H+-ATPase complex did not undergo dissociation, and H+-ATPase activation  was significantly delayed.  Conclusion: Our findings indicate that themechanism of H+-ATPase activation by glucose involves a decrease in the cytosolic pH and consequent activation of a serine protease that hydrolyzes AcTub, accelerating the process of the AcTub\/H+-ATPase complex dissociation and the activation of the enzyme.  General significance: Our data sheds light into the mechanism by which acetylated tubulin dissociates from the yeast H+-ATPase, identifying a degradative step that remained unknown. This finding also proposes an indirect way to pharmacologically regulate yeast H+-ATPase activity and open the question about mechanistic similarities with other higher eukaryotes.","palabras_clave":"H+-ATPase|Tubulin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63059,"idioma_id":4,"titulo":"On Oskar Lange's theoretical positions on equilibrium and capital in some 1930s and 1940s writings","resumen":"The paper addresses the hypothesis according to which the traditional equilibrium notion as a centre of gravitation, i.e. a position that the economy tends to realize over sufficient time under time-consuming adjustments and that forcibly needs a value specification of capital, still prevailed in neoclassical theory even after John Hick´s 1939 approach became dominant within that theory. This is shown by examining Oskar Lange´s work on equilibrium and capital, which has led us to conclude that despite he adopted the Hicksian approach in his 1944 essay, Lange continued to reason in terms of those traditional conceptions of equilibrium and capital.","palabras_clave":"capital Theory|centre of gravitation |neo-Walrasian equilibrium |Oskar Lange"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63060,"idioma_id":4,"titulo":"Biodegradable Films Containing Clove or Citronella Essential Oils against the Mediterranean Fruit Fly Ceratitis capitata (Diptera: Tephritidae)","resumen":"A concentration-response bioassay against the Mediterranean fruit fly, Ceratitits capitata, was performed to evaluate the insecticidal activity of biodegradable films containing clove (Eugenia caryophyllata, Myrtaceae) or citronella (Cymbopogon nardus, Poaceae) oils. We recorded the time of knock down flies after the exposure to soy protein-lignin films incorporated with 3% food grade clove or citronella essential oils, or control films without bioactive. The vapors of clove released from the film containing 3% essential oil were efficient to trigger the tumbling of the flies (40% after 4 h exposition) and after 20 h 90% of the flies died. However, films containing citronella essential oil had poor insecticidal activity. Citronella oil have a much higher insecticidal activity in a pure form, but probably one or both of their main bioactive compounds (citronellal and geraniol) were poorly released from the films. In this work, for the first time, a novelpackaging system controlling the release of essential oil of clove with good insecticidal activity against Ceratitits capitata was achieved, that can be used to improve quality of fruit and for food products.","palabras_clave":"BIODEGRADABLE FILMS | DIPTERA|CYMPOGON NARDUS |EUGENIA CARYOPHYLLATA | INSECTICODAL ACTIVITY|ESENTIAL OIL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63061,"idioma_id":4,"titulo":"A New Quantitative Method to Determine the Uptake of SPIONs in Animal Tissue and Its Application to Determine the Quantity of Nanoparticles in the Liver and Lung of Balb-c Mice Exposed to the SPIONs","resumen":"We propose a new method for determining the quantity of superparamagnetic iron oxide nanoparticles (Fe3O4, SPIONs) embedded in animal tissue using magnetization measurements. With this method, the smallest detectable quantity of magnetite nanoparticles in a tissue sample is 1 &#956;g. We showed that this method has proved being efficient. In this study, we focused in determining the quantity of SPION confined in lung and liver tissue of mice injected with 13 nm magnetite superparamagnetic nanoparticles. Furthermore, the method allowed us to detect the magnetite nanoparticles present in animal tissues without letting the natural iron ions present in the tissue or blood interfere with the measurements.","palabras_clave":"SPIONS Quantification| Superparamagnetism|Magnetic Nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63062,"idioma_id":4,"titulo":"Preparation of iron oxide nanoparticles stabilized with biomolecules: Experimental and mechanistic issues","resumen":"Nanoparticles (NPs) with magnetic properties based on magnetite (Fe3O4, MAG) modified with oleic acid (OA), chitosan (CS) and bovine serum albumin (BSA) have been prepared. A versatile method of synthesis was employed, involving two steps: (i) co-precipitation of MAG; and (ii) nanoprecipitation of macromolecules on as-formed MAG NPs. Experimental variables have been explored to determine the set of conditions that ensure suitable properties of NPs in terms of their size, functionality and magnetic properties. It was found that the presence of OA in Fe+2\/Fe+3 solutions yields MAG NPs with lower aggregation levels, while increasing initial amounts of OA may change the capability of NPs to disperse in aqueous or organic media by modifying the stabilization mechanism. Incorporation of CS was verified through Fourier transform IR spectroscopy. This biopolymer stabilizes NPs by electrostatic repulsions leading to stable ferrofluids and minimal fraction of recoverable solid NPs. BSA was successfully added to NP formulations,increasing their functionality and probably their biocompatibility. In this case too stable ferrofluids were obtained, where BSA acts as a polyelectrolyte. From the proposed methodology it is possible to achieve a wide range of NPs magnetically active intended for several applications. The required properties may be obtained by varying experimental conditions.             ","palabras_clave":"Magnetic nanoparticles|Co-precipitation|Chitosan|Oleic acid|Bovine serum albumin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63063,"idioma_id":4,"titulo":"Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history","resumen":"Sunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collected from Dipsacus fullonum (-dip) and Ibicella lutea (-ibi) were determined from three overlapping cDNA clones and subjected to phylogenetic and recombination analyses. SuCMoV-dip and -ibi genomes were 9,953-nucleotides (nt) long; their sequences contained an open reading frame of 9,561 nucleotides, which encoded a polyprotein of 3,187 amino acids flanked by a 50-noncoding region (NCR) of 135 nt and a 30-NCR of 257 nt. SuCMoVdip and -ibi genome nucleotide sequences were 90.9 identical and displayed 90 and 94.6 % identity to that of SuCMoV-C, and 90.8 and 91.4 % identity to that of SuCMoV-CRS, respectively. P1 of SuCMoV-dip and \u2013ibi was 3-nt longer than that of SuCMoV-CRS, but 12-nt shorter than that of SuCMoV-C. Two recombination events were detected in SuCMoV genome and the analysis of dN\/dS ratio among SuCMoV complete sequences showed that the genomic regions are under different evolutionary constraints, suggesting that SuCMoV evolution would be conservative. Our findings provide evidence that mutation and recombination would have played important roles in the evolutionary history of SuCMoV.","palabras_clave":"Potyvirus|Complete genomes|SuCMoV|Phylogenetic analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63064,"idioma_id":5,"titulo":"Comentario bibliográfico del libro de Eduardo Weisz: Racionalidad y tragedia. La filosofía histórica de Max Weber","resumen":"El trabajo es un comentario bibliográfico del libro de Eduardo Weisz: 'Racionalidad y tragedia. La filosofía histórica de Max Weber\u201D","palabras_clave":"WEBER|RACIONALIDAD|FILOSOFÍA DE LA HISTORIA|RACIONALIZACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63065,"idioma_id":4,"titulo":"Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity","resumen":"Baculoviruses (Bvs) potentiate the immune response against soluble antigens. We investigated whether Bv could be used as immunoactivator in foot-and-mouth disease (FMD) vaccines using the BALB\/c mouse model.    Mice were vaccinated with a single dose of inactivated FMDV (iFMDV), iFMDV + Bv, Bv, or culture medium. Humoral and cellular immune responses were higher in animals immunized with iFMDV + Bv than in mice vaccinated with iFMDV alone. Animals receiving iFMDV + Bv had significantly lower viremia at 2, 4 and 7 dpv, than those immunized with iFMDV alone.  In order to prolong the immune response, iFMDV oil vaccine was co-inoculated with Bv. Animals receiving iFMDV oil vaccine + Bv were protected two days earlier than those receiving the iFMDV oil vaccine alone. Both formulations protected until 14 dpv, the last day of the experiment.  This is the first report in which Bv is used as an adjuvant in a FMDV vaccine.","palabras_clave":"Baculovirus|FMDV|Early immunity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63066,"idioma_id":4,"titulo":"Experimental study of solute dispersion in macroscopic suspension  flow","resumen":"We experimentally investigate the influence of suspended   neutrally-buoyant particles on the dispersion of a passive solute in   pressure-driven axial flow in a constant aperture fracture (parallel-  plates configuration). A dye is employed as solute in order to measure   its local concentration by means of a light transmission technique. In   the experiments a dyed particle suspension displaces a transparent one at   constant flow rate, for volume fractions fi ranging from 0 to 0.25 and   for solute Péclet numbers (Pes) between 35 and 476 (mean flow velocities   U between  0.004 and 0.0544 cm\/s). The local time variation of the solute   concentration in the measurement zone was well-fitted by the solution of   the advection-dispersion equation, and a longitudinal dispersion   coefficient D for the solute was measured. For Pes &lt; 300, the values of   D for flow with particles (fi&gt;0) and without particles (fi=0) are equal   within the measurement error. For Pes &gt; 300, D decreases for fi&gt;0   compared to fi=0. The magnitude of the reduction increases as \/fi   increases, and also as Pe_s increases. This decrease of D in the presence   of suspended macroscopic particles is analyzed in the light of   theoretical, numerical and experimental results from other authors that   studied suspension flow in similar geometries.","palabras_clave":"Suspension|Dispersion|Solute|Transport"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63067,"idioma_id":4,"titulo":"Estrogen receptor-alpha  mediates human multidrug resistance associated protein 3 induction by 17-alpha-ethynylestradiol. Role of activator protein 1.","resumen":"Previously,  we  have  demonstrated  that  17a-ethynylestradiol  (EE)  induces  rat  multidrug-resistance associated  protein  3  (Mrp3,  Abcc3)  expression  transcriptionally  through  estrogen  receptor-a  (ER-a) activation.  We  explored  the  effect  of  EE  on  MRP3  expression  of  human  origin.  HepG2  cells  were transfected  with  ER-a  and  incubated  with  EE  (1?10?50  mM)  for  48  h.  MRP3  protein  and  mRNA  levels were  measured  by  Western  blotting  and  Real  time  PCR,  respectively.  EE  up-regulated  MRP3  protein  and mRNA  at  50  mM  only  in  ER-a(+)-HepG2  cells.  The  in  silico  analysis  of  mrp3  promoter  region demonstrated  absence  of  estrogen  response  elements,  but  showed  several  Ap-1  binding  sites.  We  further evaluated  the  potential  involvement  of  the  transcription  factors  c-JUN  and  c-FOS  (members  of  Ap-1)  in MRP3  up-regulation.  ER-a(+)  HepG2  cells  were  incubated  with  EE  and  c-FOS  and  c-JUN  levels  measured by  Western  blotting  in  nuclear  extracts.  EE  up-regulated  only  c-JUN.  Experiments  of  overexpression  and knock-down  of  c-JUN  by  siRNA  further  demonstrated  that  this  transcription  factor  is  indeed  implicated in  MRP3  upregulation  by  EE.  Co-immunoprecipitation  assay  demonstrated  that  EE  induces  c-JUN\/ER-a interaction,  and  chromatin  immunoprecipitation  assay  showed  that  this  complex  is  recruited  to  the  AP-1 binding  consensus  element  present  at  the  position  (1300\/1078  bp)  of  human  mrp3  promoter.  We conclude  that  EE induces  MRP3  expression  through  ER-a,  with  recruitment  of  ER-a  in  complex  with  c-JUN  to  the  human  mrp3  promoter.","palabras_clave":"MRP3|AP1|ETHYNYLESTRADIOL|ESTROGEN RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63068,"idioma_id":4,"titulo":"Hormonal regulation of hepatic drug biotransformation and transport systems","resumen":"The human body is constantly exposed to many xenobiotics including environmental pollutants, food additives, therapeutic drugs, etc. The liver is considered the primary site for drug metabolism and elimination pathways, consisting in uptake, phase I and II reactions, and efflux processes, usually acting in this same order. Modulation of biotransformation and disposition of drugs of clinical application has important therapeutic and toxicological implications. We here provide a compilation and analysis of relevant, more recent literature reporting hormonal regulation of hepatic drug biotransformation and transport systems. We provide additional information on the effect of hormones that tentatively explain differences between sexes. A brief discussion on discrepancies between experimental models and species, as well as a link between gender-related differences and the hormonal mechanism explaining such differences, is also presented. Finally, we include a comment on the pathophysiological, toxicological and pharmacological relevance of these regulations.","palabras_clave":"BIOTRANSFORMATION SYSTEMS|ABC TRANSPORTERS|GENDER DIFFERENCES|HORMONAL REGULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63069,"idioma_id":4,"titulo":"Cu(Nor)2.5H2O, a complex of Cu(II) with Norfloxacin : theoretical approach and biological studies. Cytotoxicity and genotoxicity in cell cultures","resumen":"Norfloxacin is a fluoroquinolone antibiotic used in the treatment of bacterial infections. In this article, we studied the potential antitumoral action of a complex of Norfloxacin with Cu(II), Cu(Nor)2_5H2O on osteosarcoma cells (UMR106) and calvaria-derived cells (MC3T3-E1),  evaluating its cytotoxicity and genitoxicity. We have also elucidated the more stable conformation of this complex under physiologic conditions by Molecular Dynamic simulations based on the model of the canonical ensemble and PM6 force field. When solvent effect was taken into account,  the complex conformation with both carbonyl groups in opposite sides displayed lower energy. Cu(Nor)2_5H2O caused an inhibitory effect on the proliferation on both cell lines from 300 lM (P&#65533;.01). Nevertheless, the decline on cell proliferation of UMR106 cells was more pronounced (45 % vs basal) than in MC3T3-E1 cells (20 % vs basal) at 300 lM (P&#65533;.01). Cu(Nor)2_5H2O altered lysosomal metabolism (Neutral Red assay) in a dose-dependent manner from 300 lM (P&#65533;.001). Morphological studies showed  important transformations that correlated with a decrease in the number of cells in a dose-dependent manner. Moreover, Cu(Nor)2_5H2O caused statistically significant genotoxic effects on both osteoblast cell lines in a lower range of concentrations (Micronucleus assay) (P&#65533;.05 at 10 lM,  P&#65533;.001 from 25 to 50 lM). UMR106 cells displayed a dose-related  enotoxic effect between 5 and 25 lM while the MC3T3-E1 cells showed a narrower concentration dependent range. Altogether, these results suggest that Cu(Nor)2_5H2O is a good candidate to be further evaluated for alternative therapeutics in cancer treatment.","palabras_clave":"COPPER|NORFLOXACIN|CYTOTOXICITY|GENOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63070,"idioma_id":4,"titulo":"QTL detection for fruit shelf life and quality traits across segregating populations of tomato","resumen":"The aim of this work was to detect QTLs associated with fruit shelf life and quality traits across different segregating populations derived from an interspecific cross. The first backcross generation (BC1) of an interspecific tomato cross was analyzed to detect genomic regions from Solanum pimpinellifolium accession LA722 that could improve fruit quality and prolonging fruit shelf life in an Argentinean cultivar. Families derived from five BC1 self-plant and BC2 were used to validate the detected QTL in the BC1 as well as to identify regions with wild type recessive alleles of QTLs controlling these traits. Thirty polymorphic markers (SSR) in parental genotypes and F1 were used to analyze the segregating populations. The comparison among QTLs detected in the BC1 and BC2 generations and the families BC1S1 allowed assessing the consistency of six QTLs for length, shape, weight, pH, soluble solids content and fruit shelf life. QTLs with recessive effects from wild parent prolonging fruit shelf life were found and it was possible to detect QTLs for quality traits that have not been previously reported. This finding provides alternative genes for breeding programs that attempt to improve the color, soluble solids content and fruit shelf life of tomato fruits.","palabras_clave":"BAKCROSSES|GENETIC RESOURCES|MOLECULAR MARKERS|POSTHARVEST TRAITS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63071,"idioma_id":4,"titulo":"Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform","resumen":"Trypanosoma cruzi, the etiological agent of Chagas? disease, is an early divergent eukaryote  in which control of gene expression relies mainly in post-transcriptional mechanisms.  Transcription levels are globally up and down regulated during the transition between  proliferating and non-proliferating life-cycle stages. In this work we characterized a nuclear  adenylate kinase isoform (TcADKn) that is involved in ribosome biogenesis. Nuclear  adenylate kinases have been recently described in a few organisms, being all related to  RNA metabolism.  Depending on active transcription and translation, TcADKn localizes in the nucleolus or the  cytoplasm. A non-canonical nuclear localization signal was mapped towards the N-terminal  of the protein, being the phosphate-binding loop essential for its localization. In addition,  TcADKn nuclear exportation depends on the nuclear exportation adapter CRM1. TcADKn  nuclear shuttling is governed by nutrient availability, oxidative stress and by the equivalent in  T. cruzi of the mammalian TOR (Target of Rapamycin) pathway. One of the possible  biological functions of TcADKn is ribosomal 18S RNA processing by direct interaction with  ribosomal protein TcRps14. Finally, TcADKn expression is regulated by its 3? UTR mRNA.  Depending on extracellular conditions, cells modulate protein translation rates regulating  ribosome biogenesis and nuclear adenylate kinases are probably key components in these  processes.","palabras_clave":"TRYPANOSOMA|ADK|RIBOSOME|BIOGENESIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63072,"idioma_id":4,"titulo":"Phylogenetic relationships of Culex (Culex) species (Diptera, Culicidae) from Argentina based on morphological characters","resumen":"The purpose of this study was for the first time to establish phylogenetic hypotheses among Culex (Culex) species from  Argentina, and to evaluate the relationships of this subgenus with subgenera Phenacomyia and Phytotelmatomyia. We employed  maximum parsimony to analyse morphological characters derived from the morphology of the adult, pupal and  larval stages. More than 30 figures, including photographs, illustrating 87 characters are provided. The cladistic analysis  of 61 terminal taxa and 95 characters was undertaken, under implied weighting method, with a range of K values from 6  to 9. Three unstable taxa were detected therefore a reduced strict consensus tree was created. The subgenus Culex appears  polyphyletic relative to subgenus Oculeomyia. The internal classification of subgenus Culex is shown to be chaotic; the  Pipiens Group is polyphyletic relative to the Sitiens and Coronator Groups, the latter being polyphyletic with respect to  the Pipiens Group. Phylogenetic analysis combining characters provided by morphology and molecular sources are needed  to interpret more fully the relationships in the group.","palabras_clave":"Phylogeny|Mosquitoes|Cladistic|Morphological features"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63073,"idioma_id":4,"titulo":"First report of seroprevalence of Toxoplasma gondii and Neospora caninum in dairy sheep from Humid Pampa, Argentina","resumen":"The aim of this study was to describe the occurrenceof antibodies to Toxoplasma gondii and Neosporacaninum in dairy sheep from the Humid Pampa region,Argentina. Blood samples from 704 dairy sheep belongingto six flocks were collected. Using a cut off titer of 1:50, anindirect fluorescence antibody test was used. Antibodies toT. gondii or N. caninum were detected in 17.3 % (n=122)and 3 % (n=21), respectively. All the flocks had at least oneseropositive animal to T. gondii but two of them had noseropositive sheep to N. caninum. Fifty-two of 122 (42.6 %)positive samples to T. gondii had antibody titers higher than1:400. There was a significantly higher proportion of T.gondii seropositive animals in females and older sheep(p&lt;0.05). Ten of 21 (52.3 %) positive samples to N.caninum had antibody titers higher than 1:400. This is thefirst report of seroprevalence of T. gondii and N. caninum indairy sheep from Humid Pampa, Argentina. Further researchis required for a better understanding of the role of toxoplasmosisand neosporosis in dairy sheep in Argentina.","palabras_clave":"Neospora|Toxoplasma|serology|sheep"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63074,"idioma_id":4,"titulo":"Dose-dependent immunogenicity of a soluble Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin\/&#946;-glucan adjuvant in cattle","resumen":"tMice immunized with a soluble extract of Neospora caninum tachyzoites (sNcAg) formulatedwith Providean-AVEC®, an aqueous soy-based adjuvant, are fully protected from N. caninummultiplication. Here we evaluated the dose-dependent immunogenicity of this vaccine for-mulation in cattle. Cattle (N = 3 per group) were immunized with two applications (30 daysapart) of formulations containing Providean-AVEC®and different payloads of sNcAg (100,50 and 10 g), that were five to fifty times lower than the only reported study using thissame antigen in cattle. Kinetics and magnitude of the vaccine-induced immune responseswere dose-dependent. Cattle immunized with 100 g-sNcAg elicited high-avidity specificantibodies 3 weeks after the primary vaccination while those that received 50 g of anti-gen had maximum levels of specific high-avidity antibodies 5 days after the day 30 boost.Vaccination with 10 g of sNcAg induced comparable antibody responses after 2 weekspost re-vaccination. IgG1 was the predominant isotype in all vaccinated animals. Maxi-mum systemic IFN- levels were measured in cattle immunized with 50 and 100 g-sNcAg(14 ± 2.8 ng\/ml). CD4+-T cells from vaccinated animals proliferated after sNcAg stimulationin vitro, producing IFN-. Recall IFN- responses mediated by CD4+-T cells were detected upto 140 days post vaccination. Formulations containing Providean-AVEC®and 50 g of sNcAgstimulated broad cellular and humoral immune responses against N. caninum in cattle. Theprofile and magnitude of the immune response elicited by this vaccine can be modified bythe antigen-dose and vaccination schedule. This is the first dose?response study performedin cattle using sNcAg as antigen","palabras_clave":"Neospora caninum|Adjuvant|Vaccine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63075,"idioma_id":4,"titulo":"Identification and physiological characterization of phosphatidic acid phosphatase enzymes involved in triacylglycerol biosynthesis in Streptomyces coelicolor","resumen":"BACKGROUND:Phosphatidic acid phosphatase (PAP, EC 3.1.3.4) catalyzes the dephosphorylation of phosphatidate yielding diacylglycerol (DAG), the lipid precursor for triacylglycerol (TAG) biosynthesis. Despite the importance of PAP activity in TAG producing bacteria, studies to establish its role in lipid metabolism have been so far restricted only to eukaryotes. Considering the increasing interest of bacterial TAG as a potential source of raw material for biofuel production, we have focused our studies on the identification and physiological characterization of the putative PAP present in the TAG producing bacterium Streptomyces coelicolor.RESULTS:We have identified two S. coelicolor genes, named lpp&#945; (SCO1102) and lpp&#946; (SCO1753), encoding for functional PAP proteins. Both enzymes mediate, at least in part, the formation of DAG for neutral lipid biosynthesis. Heterologous expression of lpp&#945; and lpp&#946; genes in E. coli resulted in enhanced PAP activity in the membrane fractions of the recombinant strains and concomitantly in higher levels of DAG. In addition, the expression of these genes in yeast complemented the temperature-sensitive growth phenotype of the PAP deficient strain GHY58 (dpp1lpp1pah1). In S. coelicolor, disruption of either lpp&#945; or lpp&#946; had no effect on TAG accumulation; however, the simultaneous mutation of both genes provoked a drastic reduction in de novo TAG biosynthesis as well as in total TAG content. Consistently, overexpression of Lpp&#945; and Lpp&#946; in the wild type strain of S. coelicolor led to a significant increase in TAG production.CONCLUSIONS:The present study describes the identification of PAP enzymes in bacteria and provides further insights on the genetic basis for prokaryotic oiliness. Furthermore, this finding completes the whole set of enzymes required for de novo TAG biosynthesis pathway in S. coelicolor. Remarkably, the overexpression of these PAPs in Streptomyces bacteria contributes to a higher productivity of this single cell oil. Altogether, these results provide new elements and tools for future cell engineering for next-generation biofuels production","palabras_clave":"Oleaginous bacteria| Triacylglycerol|Lipid metabolism|Phosphatidic acid phosphatase"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63076,"idioma_id":4,"titulo":"Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus","resumen":"The exposure to endocrine disrupters and female reproductive tract disorders has not been totally clarified.  The present study assessed the long-term effect of perinatal (gestation + lactation) exposure to diethylstilbestrol (DES) on the rat uterus and the effect of estrogen replacement therapy. DES (5 g\/kg bw\/day) was administered in the drinking water from gestational day 9 until weaning and we studied the uterus of young adult (PND90) and adult (PND360) females. To investigate whether perinatal exposure to DES modified the uterine response to a long-lasting estrogen treatment, 12-month-old rats  exposed to DES were ovariectomized and treated with 17-estradiol for 3 months (PND460). In young adult rats (PND90), the DES treatment decreased both the proliferation of glandular epithelial cells and the percentage of glandular perimeter occupied by -smooth muscle actin-positive cells. The other tissue compartments remained unchanged. Cell apoptosis was not altered in DES-exposed females. In control adult rats (PND360), there were some morphologically abnormal uterine glands. In adult rats exposed to DES, the incidence of glands with cellular anomalies increased. In response to estrogens (PND460), the incidence of cystic glands increased in the DES group. We observed glands with daughter glands and conglomerates of glands only on PND460 and in response to estrogen replacement therapy, independently of DES exposure. The p63 isoforms were expressed without changes on PND460. Estrogen receptors  and  showed no changes, while the progesterone receptor decreased in the subepithelial stroma of DESexposed animals with estrogen treatment. The long-lasting effects of perinatal exposure to DES included the induction of abnormalities in uterine tissues of aged female rats and an altered response of the adult uterus to estradiol.","palabras_clave":"DIETHYLSTILBESTROL |UTERUS|STEROID RECEPTORS|UTERINE GLANDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63077,"idioma_id":4,"titulo":"Acid sites development on Cr3+\/SiO2 catalysts obtained by the sol-gel method and hydrothermal treatment: Effect of calcination temperature","resumen":"Cr\/SiO2 catalysts (4\u20138% Cr, w\/w), obtained from Cr(III) salts and tetraethylortosilicate (TEOS), were preparedby the sol\u2013gel method. After TEOS hydrolysis in ammoniacal solution (pH: 9.5), the gel was treatedunder hydrothermal conditions at 120\u2013220 &#9702;C. The solids were characterized by N2 sorptometry, TG\u2013DTA,DRUV\u2013vis, XRD, and FTIR. In vacuum or N2 atmosphere, Cr3+ ions were stable up to 450 &#9702;C. However, calcinationin air promotes the partial oxidation of Cr3+ to Cr6+ as the temperature increases from 200 to450 &#9702;C. The amount of Cr6+ formed depends on the temperature of the hydrothermal treatment. 2,6-Lutidine adsorption allows to determine the evolution, with respect to calcination temperature, of bothLewis and Brönsted acid centers. Cr3+ ions were related to the presence of Lewis sites, and Cr6+ ions to thedevelopment of weak Brönsted acid sites. Accordingly, the 2-propanol dehydration reaction rate showsan increase when the catalysts are treated in N2 at 450 &#9702;C, while catalysts calcined in air develop a loweracidity. The dehydration reaction is attributed to the presence of Lewis acid centers.© 2013 Elsevier B.V. All rights reserved.","palabras_clave":"Cr\/SiO2|Sol-Gel|DRUV-vis|2,6-Lutidine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63078,"idioma_id":4,"titulo":"Overexpression of the Insulin-like growth factor 1 receptor (IGF-1R) is associated with malignancy in familial pheochromocytomas and paragangliomas","resumen":">Context: Pheochromocytomas and paragangliomas (pheo\/pgl) are  neuroendocrine tumors derived from chromaffin cells. Although mostly benign, up  to 26% of pheo\/pgl will undergo malignant transformation.Reliable histological signs to differentiate  benign pheo\/pgl from malignant tumors are currently lacking. Increased  IGF-1R expression has been shown during progression to metastatic phenotypes of  several types of cancer. Objective: to analyze the distribution and expression  of the IGF-1R in pheo\/pgl of different genetic origin and degree of malignancy.  Measurements: we studied the expression of the IGF-1R protein by immunohistochemistry,  in 40 primary tumors from patients with pheo\/pgl from different genetic  etiology (11\/29 metastatic\/non-metastatic disease). Results: We found a strong  association between increased expression of IGF-1R and malignant behavior  regardless the age at diagnosis and the genetic etiology. IGF-1R labeling was  mostly weak in primary tumors from patients with non-metastatic pheo\/pgl.  Conversely, intense IGF-1R labeling was predominant in cases of pheo\/pgl with  confirmed metastatic disease. The risk of metastases was 11.7 times higher if tumor  IGF-1R labeling was intense independently of age at diagnosis. The probability  of remaining free of metastases was higher in patients with pheo\/pgl scored  weak for IGF-1R at 60 months and more than two fold higher at 120 months of  follow-up than in patients with intense IGF-1R labeling in their primary  tumors. Conclusions: Our results strongly  suggest that IGF-1R is associated with malignancy in familial pheo\/pgl and  that IGF-1R expression in the primary tumor might be a useful tool to detect  those patients harboring pheo\/pgl that have an increased risk of metastasis.","palabras_clave":"Pheochromocytoma|Paraganglioma|IGF-1R|metastasis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63079,"idioma_id":4,"titulo":"Transmission of endemic St Louis encephalitis virus strains by local Culex quinquefasciatus populations in Co ´ rdoba, Argentina","resumen":"Background St Louis encephalitis virus (SLEV) is a re-emerging human pathogen widely distributed in the American continent. Although it is not fully understood, the SLEV transmission network may involve Culex quinquefasciatus mosquitoes as vectors and Columbidae species as hosts.    Methods To calculate infection rates, we inoculated Cx. quinquefasciatus mosquitoes from Córdoba, Argentina by feeding them on viremic chicks.    Results We observed differences in infection rate among the viral strains, the highest rate (78\/87 mosquitoes, 90.8%) being seen in strain 78V-6507. After re-feeding on susceptible chicks, mosquitoes were able to transmit the virus.    Conclusion Our findings suggest that Cx. quinquefasciatus populations are susceptible to and able to transmit different SLEV strains.","palabras_clave":"St Louis encephalitis virus|Culex quinquefasciatus Mosquitoes |Vector competence |Córdoba, Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63080,"idioma_id":4,"titulo":"Proposal to conserve the name Capparis baducca (Capparaceae) with a conserved type","resumen":"There is still controversy and debate regarding the application of the Linnaean name Capparis baducca. The underlying taxon was recognized for the first time by Linnaeus in his work Hortus Cliffortianus (1738: 204) with the following phrase-name: 'Capparis inermis, foliis ovato-oblongis per spatia confertis perennantibus', thus unmistakably indicating unarmed plants with clustered leaves. Subsequently this phrase-name was scarcely modified in Linnaeus\u2019s Species Plantarum (1753: 504): 'Capparis inermis, foliis ovato-oblongis determinate confertis perennantibus'. He marked this phrase-name with an asterisk in Species Plantarum (1753: 504), thus indicating that it was a good description, according to Stearn (in Ray Soc. Publ. 140: 162. 1957). Linnaeus did not explicitly mention a herbarium specimen for this Capparis, as he did for some species published in the Species Plantarum, but he did cite one illustration by Rheede in the protologue.","palabras_clave":"Capparis frondosa|Capparis rheedei|lectotipo|Linneo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63081,"idioma_id":4,"titulo":"Genetic and Morphometric markers are able to differentiate three morphotypes belonging to Section Algarobia of genus Prosopis (Legumi-nosae, Mimosoideae)","resumen":"The section Algarobia of genus Prosopis includes promising species for reforestation and afforestation programmes in arid and semiarid regions, mainly of the Americas. Many interspecific natural hybrid combinations have been described in this group. In this paper we analysed a hybrid zone in Chaco biogeographical province in Argentina, where P. ruscifolia and P. alba overlap and hybridise producing intermediate fertile hybrid forms. Eleven morphological traits and 76 loci RAPD were analysed to determine the effect of hybridization between these species. The comparison of morphological traits among groups yielded significant or highly significant differences for all traits. Estimates of He in P. alba and P. ruscifolia did not differ from each other, but both showed significantly lower values than the hybrid group. The analysis of correlations between shared phenotypes and pair-wise relationships estimated from RAPD gave also strong support to the hypothesis that most of the phenotypic traits analysed have significant heritability. The analyses of population structure and clustering based on morphological and molecular data by DAPC and STRUCTURE were rather consistent and indicated that the three morphotypes studied here are differentiated with low overlapping. All results indicated that despite the occurrence of natural hybridization and introgression, interspecific gene flow would be limited by hybrid breakdown or natural selection favouring the maintenance of species integrity.","palabras_clave":"Prosopis  |RAPD markers  |Morphometry  |Hybridization  |DAPC  |Structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63082,"idioma_id":4,"titulo":"Microhardness, Corrosion Behaviour and Microstructures of Directionally Solidified Al-Cu Alloys","resumen":"The aim of this work is to analyze the effect of microstructural parameters (secondary dendritic arm spacings) on the microhardness and corrosion behavior of hypoeutectic Al-Cu alloys. Experimental results include HV microhardness values, corrosion and pitting potential and current density. It was found that high cooling rates during solidification provide finer dendritic spacings, which encourage better mechanical properties. The Vickers microhardness increases as the contents of copper in the alloy increases. The most influencing microstructural variable for corrosion resistance has been found to be the secondary dendrite arm spacing. It has been found that that corrosion resistance decreases with the increasing insecondary spacing until 10wt.% of Cu.","palabras_clave":"Al-Cu alloys|directional solidification|mechanical properties|corrosion behavior"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63083,"idioma_id":4,"titulo":"Evaluation of neophobia and its potential impact upon predator control techniques: A study on two sympatric foxes in southern Patagonia","resumen":"An alternative approach to increase the efficiency of predator control and selectivity is to consider the  natural behavioural repertoire of the target species and how such behaviours may increase their vulnerability.  Neophobia, or the hesitancy to approach a novel food item, object, or place, is an important  factor influencing the investigative behaviour of animals, and its incorporation to predator control techniques  may help to reduce losses of livestock to predators. In this study, we simultaneously evaluated  the existence and intensity of neophobic responses in two sympatric fox species, the Culpeo (Pseudalopex  culpaeus) and the Grey (P. griseus) foxes in southern Patagonia, Argentina. For this purpose, we used bait  stations to compare fox behavioural responses in the absence (pre-treatment), presence (treatment) and  removal (post-treatment) of a novel stimulus, which consisted of an orange PVC-traffic cone. Both fox  species showed a neophobic response: bait-station visitation rates decreased (P = 0.005 and P = 0.048, for  Culpeo and Grey foxes, respectively) in the presence of the novel object. The intensity of the response  differed between species being higher for Culpeo foxes (approximately 80% of reduction in visitation rate  during treatment for Culpeo foxes vs. 10% for Grey foxes). However, the bait-station visitation pattern  after novel object removal indicated that animals probably increased exploration of the station. The high  level of neophobia achieved by the Culpeo fox, together with an increase in post-treatment site exploration,  suggests that behavioural manipulations (reduction of neophobia and its consequent increase in  risk taking) could improve selective and efficient fox control in rural areas where livestock production is  a major economic activity.","palabras_clave":"fox|neophobia;|novel stimulus|Patagonia;|Pseudalopex culpaeus|Pseudalopex griseus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63084,"idioma_id":4,"titulo":"Macroinvertebrate trophic responses to nutrient addition in a temperate stream in South America","resumen":"The present continuous anthropogenic pressure has resulted in an enhancement of nutrient inputs intorivers and streams. This situation has worsened, mainly in agricultural areas, causing an accelerated eutrophication.Macroinvertebrate feeding strategies reflect the species\u2019 adaptations to environments. For a characterizationof the macroinvertebrate trophic response to eutrophication, we added nitrogen and phosphorus into the La Chozastream and examined the gut contents of those taxa in order to analyze dietary alterations and assign each macroinvertebrateto a functional feeding group (FFG). Complementary C and N stable isotopes analysis was carriedout. The gut contents of all taxa studied contained principally detritus, but statistical analyses indicated significantdifferences before and after fertilization in some taxa. At the control site, and the treatment site before fertilization,the FFGs maintained constant proportions, the gathering-collector species being dominant in all samples. Afterfertilization the composition of the taxa at the treatment site varied. The &#948;13C isotopes values showed that most taxaused detritus as a basal resource. &#948;15N values were generally coincident with gut content analyses. These resultsprovide a prediction of the functional responses of macroinvertebrates to the environmental consequences expectedfrom accelerated land use. The functional responses constitute a powerful tool to assess eutrophication and its consequencesin temperate plain streams.","palabras_clave":"nutrients | stable isotopes|macroinvertebrates|gut contents| functional feeding groups"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63085,"idioma_id":4,"titulo":"Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster","resumen":"In insects, pre-adult stages of the life cycle are exposed to variation in temperature that may differ from that in adults. &nbsp;However, the genetic basis for adaptation to environmental temperature could be similar between the pre-adult and the adult stages of the life cycle. Here, we tested quantitative trait loci (QTL) for heat-stress survival in larvae of D. melanogaster, with and without a mild-heat-stress pre-treatment. Two sets of recombinant inbred lines derived from lines artificially selected for high and low levels of knockdown resistance to high temperature in young flies were used as mapping population. There was no apparent increase in heat-shock survival between heat-pretreated and non-pretreated larvae. There was a positive correlation between the two experimental conditions of heat-shock survival (with and without a heat pre-treatment) except for males from one set of lines. Several QTL were identified involving all three major chromosomes. Most QTL for larval thermotolerance overlapped with thermotolerance-QTL identified in previous studies for adults, indicating that heat-stress resistance is not genetically independent between life cycle stages because of either linkage or pleiotropy. The sign of the effects of some QTL-alleles differed both between the sexes and between life stages. ","palabras_clave":"CANDIDATE GENES|HEAT SHOCK|LARVAL SURVIVAL|QTL|THERMOTOLERANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63086,"idioma_id":4,"titulo":"Gastrointestinal parasites in greater rheas (Rhea americana) and lesser rheas (Rhea pennata) from Argentina","resumen":"Few data exist on the parasites of ratites, especially from regions within their natural range. It is only recently that extensive studies on the parasites of ostriches (Struthio camelus) have been published, mainly from European countries where commercial farming has expanded. Two species of ratites are native in South America: the lesser rhea also known as Darwin´s rhea (Rhea pennata) and the greater rhea (Rhea americana). Both species are considered near threatened by the IUCN and are included in the CITES? Appendices I and II, respectively. Parasitological studies have conservation implications, as they allow us to assess the risk of transmission of pathogens from farmed ratites to wild populations. In this study 92 faecal samples from greater rheas and 55 faecal samples from lesser rheas from different localities in Argentine were analyzed to determine their gastrointestinal parasites. In greater rheas the protozoa (Balantidium coli-like and Entamoeba spp.) and helminths (Fasciola hepatica and Deletrocephalus spp.). The protozoa had not previously been cited as parasites of greater rheas in South America. Cysts and\/or trophozoites of B. coli-like were found in 16.3% of the samples, while the prevalence of the remaining parasites was below 10%. Lesser rheas harbored the protozoa B. coli-like, Entamoeba spp. and Chilomastix spp. as well as F. hepatica and nematode eggs and larvae. B. coli-like cysts were found in 20.0% of the samples, while the prevalence of the other parasites remained below 5%. Some of them had not been cited as infecting lesser rheas yet.","palabras_clave":"ÑANDUES|CONSERVACION|MANEJO|PARASITOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63087,"idioma_id":4,"titulo":"Corticosterone stress response of Greater rhea (Rhea americana) during short-term road transport","resumen":"The effect of transport stress on blood corticosterone levels in captive Greater Rheas was investigated. Twelve adult individuals (7 males; 5 females) were loaded in pairs inside wooden crates and transported along a paved road for 30 min. Blood samples were taken before the individuals were introduced into the crate (baseline value) and immediately after they were unloaded (30 min after capture). To assess whether corticosterone levels were affected by the blood sampling procedure per se, another 6 (nontransport) control birds (3 males; 3 females) were also captured and sampled at the same times as their transported counterparts. Plasma corticosterone concentrations were measured using a commercially available corticosterone 125I radio-immunoassay kit. Baseline corticosterone levels were similar in the control and transported birds (9.0 ± 1.6 and 10.4 ± 0.8 ng\/mL, respectively). Transportation induced a highly significant (P < 0.001), more than 40-fold increase in the corticosterone levels (433.6 ± 35.4 ng\/mL) that was about 5 times higher (P < 0.001) than in their nontransported counterparts (88.4 ± 14.8 ng\/mL). The present findings suggest that Greater Rhea is a species highly sensitive to stressful manipulations. Both blood sampling and transportation induced highly significant adrenocortical responses. Considering that transportation is one of the unavoidable common practices in the management of Greater Rheas and, as shown in the present study, that it induces a significant 40-fold corticosterone stress response, efforts should focus on helping to generate management transport standards for optimization of the welfare of this ratite.","palabras_clave":"ÑANDUES|STRESS|MANEJO|CONSERVACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63088,"idioma_id":4,"titulo":"Parameter Optimization in the Synthesis of BZT Ceramics to Achieve Good Dielectric Properties","resumen":"The powder synthesis of barium zirconate titanate (BZT) (BaZrTiO3) from the mechanochemical activation of BaCO3, ZrO2, and TiO2 was studied. The grinding effect, by using a planetary ball milling, on the crystallization temperature of BZT powders  was analyzed. X-ray diffractometry, differential thermal analysis, thermogravimetric analysis, and scanning electronic microscopy (SEM) were used as characterization methods. The crystallization behavior of powders activated by high-energy grinding and the effect of grinding time on the BZT crystallization were analyzed. After grinding by 4&#8201;h, the BaZr(0.05)Ti(0.95)O3 sample was almost fully crystallized at . The results of dielectric and ferroelectric properties show that high-energy ball milling is a practical and promising way to prepare BZT ceramics.","palabras_clave":"BZT ceramics |Dielectric properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63089,"idioma_id":4,"titulo":"Vortices in Ionization Collisions by Positron Impact","resumen":"The presence of vortices in the ionization of hydrogen atoms by positrons at intermediate impact energies is investigated. The present findings show that a previously reported minimum in the fully differential cross section is the signature of a vortex in the continuum positron\u2013electron\u2013proton system. The behaviour of the real and imaginary parts of the complex-valued transition matrix is studied in order to determine and characterize the vortex in momentum space. The obtained information is translated to fully differential ionization cross sections, feasible of being measured with currently available techniques.","palabras_clave":"IONIZATION|POSITRON|VORTICES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63090,"idioma_id":4,"titulo":"Apsidal motion in massive close binary systems - I. HD 165052, an extreme case?","resumen":"We present a new set of radial-velocity measurements of the spectroscopic binary HD 165052 obtained by disentangling of high-resolution optical spectra. The longitude of the periastron (w = 60 +\/- 2 degrees) shows a variation with respect to previous studies. We have determined the apsidal motion rate of the system dw\/dt = 12.10+\/-0.3 &#9702;\/yr, which was used to calculate the absolute masses of the binary components: M1 = 22.5 +\/- 1 Msol and M2 = 20.5 +\/- 0.9 Msol. Analysing the separated spectra we have re-classied the components as O7Vz and O7.5Vz stars","palabras_clave":"STARS: EARLY TYPE|STARS: FUNDAMENTAL PARAMETERS|STARS: MASSIVE|STARS: INDIVIDUAL: HD165052"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63091,"idioma_id":4,"titulo":"Mechanisms of fluoride release in sediments of  Argentina's central region","resumen":"We analyze the sources and mechanisms that control the release of fluoride from sediments collected from two types of aquatic reservoirs in the central region of Argentina: 1)&nbsp; mountainous rivers draining crystalline rocks from the Sierras Pampeanas ranges, and 2) shallow aquifers in loessic sediments. The assessment was performed on the basis of experimental work and the study of chemical and mineralogical characteristics ofsediments in contact with F-rich waters of the studied region. The chemical and mineralogical compositions of sediments were analyzed by ICP\/OES, DRX, and SEM-EDS. Batch experiments were conducted to evaluate the kinetics of fluoride release under variable pH and ionic composition of the solution. The enhanced release of fluoride at more acidic pH, the inhibition of release in Ca-rich solutions and the positive significant linear trends between Ca2+ and fluoride concentrations suggest that the dissolution of a Ca\/F-bearing phase (like fluorapatite) strongly controls the dynamics of fluoride in the early stages of water\u2013sediment interaction, particularly under acidic conditions. Calculations revealed that the dissolution of an amount of FAp equivalent to that estimated in the studied sediments may widely account for the values measured in the leaching experiments at pH 6, whatever the dominant cation in the solution. Under such conditions, dissolution of FAp (present as coatings onto glass surfaces or as detritic grains) is likely the major primary source of fluorine in the studied sediments. Contribution from biotite may be also considered as a source in fluvial sediments. When adequate surfaces are present the released anions may partially be scavenged from the solution by adsorption at acidic pH. Increasing alkalinity in the aquatic reservoirs may then release the adsorbed fluoride through desorption or through competition with other anionic species. Comparing both mechanisms, dissolution predominates at lower pH while desorption is the main contribution under alkaline conditions. ","palabras_clave":"LOESS|FLUORAPATITE|DESORPTION|VOLCANIC GLASS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63092,"idioma_id":4,"titulo":"New family host and records from Argentina, and implications in the transmission mechanism of cimicid bugs among birds´nests","resumen":"Acanthocrios furnarii (Cordero &amp; Vogelsang, 1928) [Hemiptera: Cimicidae: Haematosiphoninae] is an ectoparasite onavian hosts from Argentina and Uruguay. It has been mostly found in mud nests of Furnarius rufus (Gmelin, 1788) [Aves: Furnariidae], but its true hosts are some of the inquiline birds that use F. rufus nests. These inquiline hosts belong to the families Emberizidae, Hirundinidae, Icteridae, Passeridae, and Troglodytidae. Outside F. rufus mud nests, A. furnarii has been found in nests of other Furnariidae, Hirundinidae, and Passeridae. The present work adds the first nonpasserine host (Falconidae) of A. furnarii, together with new records in La Pampa, Argentina. The transmission mechanism of A.furnarii, together with all other cimicid bugs from Argentina and adjacent countries, is increased considering this new host; and we also take into account the birds that nidificate in nest boxes, the cavity-nesting birds in trees and earth, and the inquiline birds in stick nests of Furnariidae and Psittacidae","palabras_clave":"BIRD BUGS|HEMIPTERA|CIMICIDAE|FALCONIDAE|NEW HOST|NEW RECORDS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63093,"idioma_id":4,"titulo":"Heights of varieties in multiprojective spaces and arithmetic Nullstellensätze","resumen":"We present bounds for the degree and the height of the polynomials&nbsp; arising in some problems in effective algebraic geometry&nbsp; including the implicitization of rational maps and the effective&nbsp; Nullstellensatz over a variety.&nbsp; Our treatment is based on&nbsp; arithmetic intersection theory in products of projective spaces and&nbsp; extends to the arithmetic setting constructions and results due to&nbsp; Jelonek.&nbsp; A key role is played by the notion of {canonical mixed&nbsp;&nbsp;&nbsp; heights} of multiprojective varieties. We study this notion from&nbsp; the point of view of resultant theory and establish some of its&nbsp; basic properties, including its behavior with respect to&nbsp; intersections, projections and products. We obtain analogous results&nbsp; for the function field case, including a parametric Nullstellensatz.","palabras_clave":"Multiprojective spaces|mixed heights of varieties|implicitization|arithmetic nullstellensatze"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63094,"idioma_id":4,"titulo":"Survey of Aspergillus section Flavi and aflatoxin B1 in brewers grain used as pig feedstuff in Córdoba, Argentina","resumen":"Brewing industry by-products are important animal  feedstuff alternatives for local swine producers in Córdoba,  Argentina. The high content of nutrients makes these  by-products vulnerable to bacterial and fungal contamination.  The objectives of the present study were (1) to determine  the presence of Aspergillus section Flavi in brewer´s  grain used to feed pigs and (2) to evaluate the incidence of  aflatoxin B1 in the substrate. Total fungal count of most  samples exceeded the levels proposed as feed quality limits,  and most Aspergillus section Flavi strains found were able  to produce high amounts of AFB1 in vitro. However, the  incidence of AFB1 was low. The presence of contamination  by aflatoxicogenic species in feedstuff might affect the  productivity of swine producers and indirectly represents a  public health issue.","palabras_clave":"ASPERGILLUS FLAVUS|AFLATOXIN B1|FEEDSTUFF|BREWER´S GRAINS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63095,"idioma_id":4,"titulo":"Liquid-liquid microextraction based on a dispersion of Pd nanoparticles combined with ETAAS for sensitive Hg determination in water samples","resumen":"A novel and highly efficient microextraction methodology based on the use of palladium nanoparticles (Pd NPs) was developed for the preconcentration and determination of Hg in water samples. Selective separation of the analyte was achieved by application of dodecanethiolate -coated Pd monolayer- protected clusters (C12SPdMPCs) in a liquid\u2013liquid microextraction technique (LLME). A volume of 20 mL of toluene phase containing C12SPdMPCs was used for extraction and final phase was injected in an electrothermal atomic absorption spectrometer (ETAAS) for Hg detection. The effects of different variables, such as sample volume, extraction time, and NPs dispersion volume were carefully studied. A sensitivity enchancement factor of 95 was obtained under optimal experimental conditions. Furthermore, low detection limit (7.5ng\/L) and good precision (relative standard deviation of 4.1% at 0.25 mg\/L Hg and n=10) were achieved. The proposed method can be considered as a rapid, cost-effective, and efficient alternative for Hg determination in water samples like river, lake, mineral and tap water.","palabras_clave":"Organic-coated Pd nanoparticles|Microextraction|Mercury|Preconcentration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63096,"idioma_id":4,"titulo":"Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup\u0002 (glyphosate) concentrations used in agriculture","resumen":"Caiman latirostris is one of the two crocodilian species living in Argentina. As a result of agricultural expansion produced in recent years, some populations are exposed to continuous pesticide dischargedue to the proximity of its natural geographic distribution to extensive agricultural areas. The aim of this study was to evaluate genotoxicity and the effects on growth of Roundup (RU; glyphosate basedformulation) on C. latirostris hatchlings, considering concentrations commonly applied in crops and subsequently decay of the compound in water through time, as it might occur in the environment. C. latirostris 20 days old, from three different clutches, were exposed to two RU concentrations in plastic containers, during two months. RU concentration was progressively decreased through time, so experimental groups were: treatment 1: 11 mg\/l (concentration at the beginning) to 2.5 mg\/l (concentration at the end of experiment), treatment 2: 21 mg\/l to 5 mg\/l; and a control without RU. At the end of exposure period, blood samples were obtained and the micronucleus (MN) test applied in erythrocytes as amarker of genotoxicity. Results indicated a significant increase in the frequency of MN (p < 0.05) and a tendency to lower growth in the groups exposed to RU compared to the negative control. These results, together with those reported in previous studies; warn about the effect that C. latirostris wild populations continuously exposed to low concentrations of pesticides might be suffering.","palabras_clave":"GENOTOXICITY|MICRONUCLEUS TEST|PESTICIDES|FIELD CONCENTRATIONS|GLYPHOSATE DECREASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63097,"idioma_id":4,"titulo":"The receptor-like kinase ERECTA contributes to the shade avoidance syndrome in a background-dependent manner","resumen":"      Background and Aims Plants  growing at high densities perceive a decrease in the red to far-red (R:FR)  ratio of incoming light. These changes in light quality trigger a suite of responses  collectively known as the shade avoidance syndrome (SAS) including hypocotyl and  stem elongation, inhibition of branching, and acceleration of flowering.       Methods We mapped quantitative trait loci (QTL) for the hypocotyl length to  end-of-day far-red (EOD), a simulated shade avoidance response, in recombinant  inbred line (RIL) populations of Arabidopsis  thaliana seedlings, derived from Landsberg erecta (Ler) and three accessions (Columbia, Col; Nossen, No-0; and  Cape Verde Islands, Cvi-0).       Key Results We  identified five loci responsible for the EOD response with positive  contribution of Ler alleles on the phenotype independently of the RIL  population. Quantitative complementation analysis and transgenic lines showed  that PHYB is the candidate gene for EODRATIO5 in the Ler x Cvi-0 RIL population,  but not for two colocalized QTL, EODRATIO1  and EODRATIO2 mapped in the Ler x No-0 and Ler x Col RIL  populations, respectively. The ERECTA gene was also implicated in the  SAS in a background-dependent manner. For hypocotyl length EOD response, we  found a positive contribution of erecta  alleles in Col and Van-0, but not in Ler, Cvi-0, Hir-1 and Ws. Furthermore, pleiotropic  effects of ERECTA in the EOD response  were also detected for petiole and lamina elongation, hyponastic growth, and  flowering time.      Conclusions Our results show that the analysis of multiple  mapping populations leads to a better understanding of the SAS genetic  architecture. Moreover, the background- and the trait-dependent contribution of  ERECTA in the SAS suggest that its  function in shaded natural environments may be relevant for some populations in  different phases of plant development. We propose that ERECTA is involved in canalization processes buffering the genetic  variation of the SAS against environmental light fluctuations.  ","palabras_clave":"ERECTA|Shade Avoidance Syndrome|Natural Genetic Variation|QTL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63098,"idioma_id":4,"titulo":"In vivo antigenotoxic activity of watercress juice (Nasturtium officinale L.) against induced DNA damage","resumen":"The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7?8 weeks old (N = 48) were treated by gavage with 1 g kg1 body weight and 0.5 g kg1 body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assayin peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P<0.05 and P<0.001,respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage.","palabras_clave":"WATERCRESS|ANTIGENOTOXICITY|CYCLOPHOSPHAMIDE|BLADDER|BIOMARKERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63099,"idioma_id":4,"titulo":"Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay","resumen":"From the organic extracts of the sponge Siphonochalina fortis, collected at Bahía Bustamante, Chubut, Argentina, three major compounds were isolated and identified as deoxycholic acid 3, 12-diacetate (1),cholic acid 3, 7, 12-triacetate (2) and cholic acid, 3, 7, 12-triacetate. (3). This is the first report ofacetylated bile acids in sponges and the first isolation of compound 3 as a natural product. Thepotential induction of DNA lesions by the isolated compounds was investigated using the comet assay in lymphocytes of human peripheral blood as in vitro model. The results showed that theadministration of the bile acid derivatives would not induce DNA damages, indicating that acetylated bile acids are nontoxic metabolites at the tested concentrations. Since the free bile acids were not detected, it is unlikely that the acetylated compounds may be part of the sponge cells detoxification mechanisms. These results may suggest a possible role of acetylated bile acids as a chemical defense mechanism, product of a symbiotic relationship with microorganisms, which would explain their seasonal and geographical variation, and their influence on the previously observed genotoxicity of the organic extract of S. fortis.","palabras_clave":"ACETYLATED BILE ACID|COMET ASSAY|DNA DAMAGE|MARINE SPONGES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63100,"idioma_id":4,"titulo":"Chemobiosynthesis of New Antimalarial Macrolides","resumen":"We have synthesized new derivatives of the macrolide antibiotics erythromycin and azithromycin. Novel deoxysugar moieties were attached to these standard antibiotics by biotransformation using a heterologous host. The resulting compounds were tested against several standard laboratory and clinically isolated bacterial strains. In addition, they were also tested in vitro against standard and drug-resistant strains of human malaria parasites (Plasmodium falciparum) and the liver stages of the rodent malaria parasite (Plasmodium berghei). Antibacterial activity of modified erythromycin and azithromycin showed no improvement over the unmodified macrolides, but the modified compounds showed a 10-fold increase in effectiveness after a short-term exposure against blood stages of malaria. The new compounds also remained active against azithromycin-resistant strains of P. falciparum and inhibited growth of liver-stage parasites at concentrations similar to those used for primaquine. Our findings show that malaria parasites have two distinct responses to macrolide antibiotics, one reflecting the prokaryotic origin of the apicoplast and a second, as-yet uncharacterized response that we attribute to the eukaryotic nature of the parasite. This is the first report for macrolides that target two different functions in the Plasmodium parasites.","palabras_clave":"MALARIA|MACROLIDE|BIOTRANSFORMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63101,"idioma_id":4,"titulo":"Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations","resumen":"Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molecular markers were used to evaluate 14 populations from the Instituto Nacional de Tecnología Agropecuaria (INTA) sunflower germplasm collection. These populations were chosen to represent historically important accessions that still play a central role within the INTA sunflower breeding programme due to their extensive variation in diverse agronomically important traits. Nine quantitative and eight qualitative agro-morphological descriptors were recorded and compared with those of a larger set of accessions representative of the phenotypic diversity of the sunflower collection. Molecular characterization was conducted on a total of 311 individuals using 16 microsatellite markers. Overall, the average gene diversity was 0.56 and the average number of alleles per locus was 6.25. No statistically significant differences in genetic diversity were detected between the OPs and CPs. Global estimates of F ST revealed very high levels of differentiation among accessions (F ST= 0.413, P< 0.05). Population structure analyses were consistent with the observed levels of differentiation and identified two major groups. The results of this work show that high global diversity is preserved within the accessions analysed here. Additionally, this study provides a set of reliable and discriminant markers for the cost-effective molecular characterization of sunflower accessions, along with the guidelines for the delineation of sampling strategies for OPs and CPs, thus aiding the efficient management and exploitation of sunflower germplasm collections.","palabras_clave":"sunflower|population genetics|germplam|molecular markers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63102,"idioma_id":4,"titulo":"Fine-scale genetic structure in populations of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae).","resumen":"Fine scale patterns of genetic structure and dispersal in Triatoma infestans populations from Argentina was analysed. A total of 314 insects from 22 domestic and peridomestic sites from the locality of San Martín (Capayán department, Catamarca province) were typed for 10 polymorphic microsatellite loci. The results confirm subdivision of T. infestans populations with restricted dispersal among sampling sites and suggest inbreeding and\/or stratification within the different domestic and peridomestic structures. Spatial correlation analysis showed that the scale of structuring is approximately of 400 m, indicating that active dispersal would occur within this distance range. It was detected difference in scale of structuring among sexes, with females dispersing over greater distances than males. This study suggests that insecticide treatment and surveillance should be extended within a radius of 400 m around the infested area, which would help to reduce the probability of reinfestation by covering an area of active  dispersal. The inferences made from fine-scale spatial genetic structure analyses of T. infestans populations has demonstrated to be important for community-wide control programs, providing a complementary approach to help improve vector control strategies.","palabras_clave":"TRIATOMA|CHAGAS|GENETIC|STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63103,"idioma_id":4,"titulo":"Concomitant infection of Neospora caninum and Bovine  Herpesvirus type 5 in spontaneous bovine abortions","resumen":"Bovine Herpesvirus type 5 (BoHV-5) has not been conclusively demonstrated to causebovine abortion. Brain lesions produced by Neospora caninum and Bovine Herpesvirustype 1 (BoHV-1) exhibit common features. Therefore, careful microscopic evaluation andadditional diagnostic procedures are required to achieve an accurate final etiological diagnosis.The aim of the present work was to investigate the occurrence of infections due toBoHV-1, BoHV-5 and N. caninum in 68 cases of spontaneous bovine abortions which showedmicroscopic lesions in the fetal central nervous system. This study allowed the identificationof 4 (5.9%) fetuses with dual infection by BoHV-5 and N. caninum and 33 (48.5%)cases in which N. caninum was the sole pathogen identified. All cases were negative toBoHV-1. The results of this study provide evidence that dual infection by BoHV-5 and N.caninum occur during pregnancy in cattle; however, the role of BoHV-5 as a primary causeof bovine abortion needs further research. Molecular diagnosis of BoHV-5 and N. caninumconfirmed the importance of applying complementary assays to improve the sensitivity ofdiagnosing bovine abortion.","palabras_clave":"NEOSPORA|HERPESVIRUS|ABORTO|BOVINO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63104,"idioma_id":4,"titulo":"Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents","resumen":"Abstract: Eryptosis is a process by which mature erythrocytes  can undergo self-destruction sharing several features  with apoptosis. Premature programmed erythrocyte  death may be induced by different agents. In this study, we  compared mechanisms involved in two eryptotic models  (oxidative stress and cell calcium overload) so as to distinguish  whether they share signaling pathways and could  be prevented by erythropoietin (Epo). Phosphatidylserine  (PS) translocation and increased calcium content were  common signs in erythrocytes exposed to sodium nitrite  plus hydrogen peroxide or calcium ionophore A23187  (CaI), while increased ROS and decreased GSH levels  were detected in the oxidative model. Protein kinase activation  seemed to be an outstanding feature in eryptosis  induced by oxidative stress, whereas phosphatase activation  was favored in the CaI model. Cell morphology and  membrane protein modifications were also differential  signs between both models. Epo was able to prevent cell  oxidative imbalance, thus blunting PS translocation.  However, the hormone favored intracellular calcium influx  which could be the reason why it could not completely  counteract the induction of eryptosis. Instead, Epo was  unable to inhibit PS externalization in the CaI model. The  different mechanisms involved in the eryptotic models may  explain the differential action of Epo upon erythrocytes  induced to eryptosis by different agents.","palabras_clave":"ERYPTOSIS|ERYTHROPOIETIN|OXIDATIVE STRESS|CALCIUM OVERLOAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63105,"idioma_id":4,"titulo":"Signaling pathways of cell proliferation are involved in the differential effect of erythropoietin and its carbamylated derivative","resumen":"It is now recognized that in addition to its activity upon erythroid progenitor cells, erythropoietin (Epo) is capable of stimulating survival of different non-erythroid cells. Since stimulation of erythropoiesis is unwanted for neuroprotection, Epo-like compounds with a more selective action are under investigation. Although the carbamylated derivative of erythropoietin (cEpo) has demonstrated nonhematopoietic tissue protection without erythropoietic effect, little is known about differential mechanisms between Epo and cEpo. Therefore, we investigated signaling pathways which play a key role in Epo-induced proliferation. Here we show that cEpo blocked FOXO3a phosphorylation, allowing expression of downstream target p27kip1 in UT-7 and TF-1 cells capable of erythroid differentiation. This is consistent with the involvement of cEpo in slowing down G1-to-S-phase progression compared with the effect of Epo upon cell cycle. In contrast, similar antiapoptotic actions of cEpo and Epo were observed in neuronal SH-SY5Y cells. Inhibition and competition assays suggest that Epo may act through both, the homodimeric (EpoR\/EpoR) and the heterodimeric (EpoR\/beta commonR) receptors in neuronal SH-SY5Y cells and probably in the TF-1 cell type as well. Results also indicate that cEpo needs both the EpoR and beta common R subunits to prevent apoptosis of neuronal cells. Based on evidence suggesting that cell proliferation pathways were involved in the differential effect of Epo and cEpo, we went forward to studying downstream signals. Here we provide the first evidence that unlike Epo, cEpo failed to induce FOXO3a inactivation and subsequent p27kip1 downregulation, which is clearly shown in the incapacity of cEpo to induce erythroid cell growth.","palabras_clave":"ERYTHROPOIETIN|CARBAMYLATED ERYTHROPOIETIN|FOXO3a|P27kip1|CELL PROLIFERATION|CELL CYCLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63106,"idioma_id":4,"titulo":"B cell lymphopoiesis is regulated by cathepsin L","resumen":"Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The  involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt\/nkt mice that lack  CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool  by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the  influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. Bcell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt\/nkt mice. Increases in splenic B-cell  numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in  the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt\/nkt mice. In the BM, the  percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, in  vitro and in vivo experiments showed that BM B-cell production was markedly increased in CTSLnkt\/nkt mice. Besides, BM Bcell emigration to the spleen was increased in CTSLnkt\/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence  of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to  sustain the increased B-cell hematopoiesis in CTSLnkt\/nkt mice. Overall, our data clearly demonstrate that CTSL negatively  regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.","palabras_clave":"B LYMPHOPOIESIS|CATHEPSIN L|BONE MARROW"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63107,"idioma_id":4,"titulo":"Antioxidant treatment prevents the development of fructose-induced abdominal adipose tissue dysfunction","resumen":"In the present study, we tested the effect of OS (oxidative stress) inhibition in rats fed on an FRD [fructose-rich diet; 10% (w\/v) in drinking water] for 3 weeks. Normal adult male rats received a standard CD (commercial diet) or an FRD without or with an inhibitor of NADPH oxidase, APO (apocynin; 5 mM in drinking water; CD-APO and FRD-APO).We thereafter measured plasma OS and metabolic-endocrine markers, AAT (abdominal adipose tissue) mass and cell size, FA (fatty acid) composition (content and release), OS status, LEP (leptin) and IRS (insulin receptor substrate)-1\/IRS-2 mRNAs, ROS (reactive oxygen species) production, NADPH oxidase activity and LEP release byisolated AAT adipocytes. FRD-fed rats had larger AAT mass without changes in body weight, and higher plasmalevels of TAG (triacylglycerol), FAs, TBARS (thiobarbituric acid-reactive substance) and LEP. Although no signi&#64257;cantchanges in glucose and insulin plasma levels were observed in these animals, their HOMA-IR (homoeostasis modelassessment of insulin resistance) values were signi&#64257;cantly higher than those of CD. The AAT from FRD-fed rats hadlarger adipocytes, higher saturated FA content, higher NADPH oxidase activity, greater ROS production, a distortedFA content\/release pattern, lower insulin sensitivity together with higher and lower mRNA content of LEP andIRS-1-\/2 respectively, and released a larger amount of LEP. The development of all the clinical, OS, metabolic,endocrine and molecular changes induced by the FRD were signi&#64257;cantly prevented by APO co-administration. Thefact that APO treatment prevented both changes in NADPH oxidase activity and the development of all the FRD-induced AAT dysfunctions in normal rats strongly suggests that OS plays an important role in the FRD-inducedMS (metabolic syndrome) phenotype.","palabras_clave":"ABDOMINAL ADIPOCYTE|ADIPOKINE|FRUCTOSE-RICH DIET|INSULIN SIGNALLING|LIPID METABOLISM|METABOLIC SYNDROME"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63108,"idioma_id":4,"titulo":"Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites","resumen":"Nowadays, there are no simple techniques for mimicking in vitro the life cycle ofthe kinetoplasmid Trypanosoma cruzi (Chagas 1909), causative agent of Chagas disease,especially for those parasite strains maintained as epimastigotes for many years. In the presentwork, we propose a method for obtaining metacyclic trypomastigotes, which were capable ofinfecting mammalian cells, by simply lowering pH media. The collected amastigotes andtrypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro.Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using thismethod, we were able to infect cells with transgenic parasites obtaining trypomastigotes andamastigotes using a neomycin resistant cell line.","palabras_clave":"TRYPANOSOMA CRUZI|CHAGAS DISEASE|METACYCLOGENESIS|PARASITE  DIFFERENTIATION|INTRACELLULAR EPIMASTIGOTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63109,"idioma_id":5,"titulo":"Consideraciones socioambientales sobre la cuenca del río Gallegos y Chico, provincia de Santa Cruz","resumen":"El objetivo de este artículo es analizar la distribución y función de los asentamientos humanos de la cuenca del río Gallegos y Chico, ubicada al Sur de la Provincia de Santa Cruz y relacionarlos con los componentes fisiográficos, en particular con los tipos de humedales.   Metodológicamente se relevó y analizó bibliografía científica e información oficial, relacionándola en una base de datos geográfica con el fin de visualizar los asentamientos rurales y urbanos y vincularlos con los distintos tipos de humedales.  Como resultados se destaca que la población se concentra en el tramo superior e inferior de la cuenca y que las características naturales del área permiten un alto índice de carga ganadera. Asimismo, se destaca que en los humedales presentes en el ámbito rural se reconocen actividades de uso directo, mientras que en los presentes en el ámbito urbano se identifican valores de uso indirecto.","palabras_clave":"cuenca del río Gallegos y Chico| Dinàmica demográfica|Humedales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63110,"idioma_id":4,"titulo":"Biomonitors of urban air pollution: Magnetic studies and SEM observations of corticolous foliose and microfoliose lichens and their suitability for magnetic monitoring","resumen":"This study explored the suitability of available lichen species as air pollution biomonitors and assessed their potential for magnetic monitoring in cities. Several lichens on tree bark were collected in urban and industrial sites from Tandil city, as well as control sites. The results showed that magnetite-like minerals were the main magnetic carriers in all sites and samples. However, the concentration varied between clean and polluted sites. In addition, magnetic-grain size-distribution showed clear differences between sites. Observations by scanning electron microscopy showed different particles in a variety of shapes and grain sizes; moreover, the presence of iron oxides and several toxic elements was detected by energy dispersive spectroscopy analysis. Although eleven lichen species were identified that appeared suitable for use as air-pollution monitors, three of them, Parmotrema pilosum, Punctelia hipoleucites and Dirinaria picta, occurred more frequently in the area, thus constituting appropriate species for future monitoring in the study area.","palabras_clave":"Biomonitoring|Magnetic susceptibility|Lichen|Atmospheric pollution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63111,"idioma_id":4,"titulo":"Antibacterial potential of non-volatile constituents of Rosmarinus officinalis against 37 clinical isolates of multidrug-resistant bacteria","resumen":"Rosmarinus officinalis L. and their main constituents, carnosic acid and rosmarinic acid, against 37 nosocomial strains of multidrug-resistant bacteria. Results obtained showed that both the rosemary extract and carnosic acid inhibited all clinical isolates of Staphylococcus aureus , Enterococcus faecalis gentamicin and streptomycin-resistant bacteria examined (MICs 60 ìg\/mL vs. 200 ìg\/mL, respectively). Rosemary extract showed MIC values between 400 and 1600 ìg\/ml against the Gram-negative multidrug-resistant bacteria: Proteus mirabilis, Enterobacter cloacae, Pseudomonas aeruginosa, Morganella morganii , Providencia stuartii , while carnosic acid showed MIC of 120 to 240 ìg\/mL. Bactericidal effect of carnosic acid against S. aureus and E. faecalis was observed at their MIC value, while 2 x MIC to 4 x MIC were needed to kill Gram-negative bacteria. Rosmarinic acid showed a narrow spectrum of action against a few Gram-negative clinical isolates. Our findings suggest that carnosic acid would be a good lead candidate useful in counteracting drug-resistant infections.","palabras_clave":"Antibacteriano|acido carnósico|cepas multiresistente|acido rosmarinico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63112,"idioma_id":4,"titulo":"Genes related to suppression of malignant phenotype induced by Maitake D-Fraction in breast cancer cells","resumen":"It is already known that the Maitake (D-Fraction) mushroom is involved in stimulating the immune system and activating certain cells that attack cancer, including macrophages, T-cells, and natural killer cells. According to the U.S. National Cancer Institute, polysaccharide complexes present in Maitake mushrooms appear to have significant anticancer activity. However, the exact molecular mechanism of the Maitake antitumoral effect is still unclear. Previously, we have reported that Maitake (D-Fraction) induces apoptosis in breast cancer cells by activation of BCL2-antagonist\/killer 1 (BAK1) gene expression. At the present work, we are identifying which genes are responsible for the suppression of the tumoral phenotype mechanism induced by Maitake (D-Fraction) in breast cancer cells. Human breast cancer MCF-7 cells were treated with and without increased concentrations of Maitake D-Fraction (36, 91, 183, 367 &#956;g\/mL) for 24 h. Total RNA were isolated and cDNA microarrays were hybridized containing 25,000 human genes. Employing the cDNA microarray analysis, we found that Maitake D-Fraction modified the expression of 4068 genes (2420 were upmodulated and 1648 were downmodulated) in MCF-7 breast cancer cells in a dose-dependent manner during 24 h of treatment. The present data shows that Maitake D-Fraction suppresses the breast tumoral phenotype through a putative molecular mechanism modifying the expression of certain genes (such as IGFBP-7, ITGA2, ICAM3, SOD2, CAV-1, Cul-3, NRF2, Cycline E, ST7, and SPARC) that are involved in apoptosis stimulation, inhibition of cell growth and proliferation, cell cycle arrest, blocking migration and metastasis of tumoral cells, and inducing multidrug sensitivity. Altogether, these results suggest that Maitake D-Fraction could be a potential new target for breast cancer chemoprevention and treatment","palabras_clave":"D-FRACTION|GENES|BREAST|CANCER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63113,"idioma_id":4,"titulo":"Inter-male aggression with regard to polygynous mating system in Pampean grassland mouse, Akodon azarae (Cricetidae: Sigmodontinae)","resumen":"Abstract Based on the hypothesis that, in Akodon azarae, polygyny operates through female defence, we studied inter-male aggression in order to test the following predictions: during the breeding period (1) resident males are more aggressive than intruder males in the presence offemales (FP), and (2) aggressive behaviour is independent of male condition (resident or intruder) in the absence of females (FA). To test our predictions, we used the resident male behavioural response towards an intruder male in relation to FP or FA. We conducted 30 encounters in FP and 27 in FA in 0.79-m2 round enclosures placed in the Espinal Reservation. Our results support the prediction that, in FP, the intensity of aggressive behaviour exhibited by males varied in relation to resident or intruder condition. Resident males showed high levels of aggression towards intruders, and intruders exhibited the greatest values of submissive behaviours with residents. In FA, the intensity of aggressive behaviour did not vary in relation to resident or intruder condition. Both resident and intruder males exhibited low aggressive behaviour and inter-male encounters resulted mainly in non interactive behaviours. Our results support the hypothesis that, in A. azarae, the polygynous mating system operates through female defence.","palabras_clave":"Aggressive behaviour|Cricetidae|Mating system|Polygyny"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63114,"idioma_id":4,"titulo":"Dynamics of arbuscular mycorrhizal fungi spore populations and their viability under contrasting tillage systems in wheat at different phenological stages","resumen":"Arbuscular mycorrhizal fungi (AMF) are in&#64258;uenced by soil management. The use of different tillage systems affects AMF activity. AMF spores are formed in soils or roots and provide a long-term reservoir of inoculum in the &#64257;eld. As spores can persist in soils, they re&#64258;ect the accumulated sporulation history of the respective soil and not necessarily the current symbiosis of the crop. The aim of this study was to evaluate theAMF spore population dynamics and spore viability in conventional tilled and nontilled soils throughout the wheat growing cycle and its fallow in the warm temperate Argentine Pampas. It was found that the differences in spore abundance between both tillage systems depend on the phenological stage of the crop. In both years, at the early phenological stages of the wheat crop, spore counts were about two or three times higher in no-tillage (NT) than in conventional tillage (CT). The lower spore counts inCT at the end of the fallow and at the early crop stages could be explained by the dilution of the AMF propagules in the zone of seedling establishment by ploughing. The percentages of viable spores varied with the treatments and the sampling periods, with values ranging from 10.5% to 58.8%, and were higher in CT in all the phenological stages and signi&#64257;cantly higher at tillering stage. Assuming that a viablespore could be newly formed, then the lower percentages of viable spores in NT may suggest a higher accumulation of old residual spores.","palabras_clave":"Conventional tillage|Glomeromycota|number of spores|vital stain"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63115,"idioma_id":4,"titulo":"Milky Way Demographics with the VVV Survey II. Color Transformations and Near-Infrared Photometry for 136 Million Stars in the Southern Galactic Disk","resumen":"The new multi-epoch near-infrared VISTA Variables in the Vía Láctea (VVV) survey is sampling 562&nbsp;&nbsp;&nbsp;deg2   of the Galactic bulge and adjacent regions of the disk. Accurate   astrometry established for the region surveyed allows the VVV data to be   merged with overlapping surveys (e.g., GLIMPSE, WISE, 2MASS, etc.),   thereby enabling the construction of longer baseline spectral energy   distributions for astronomical targets. However, in order to maximize   use of the VVV data, a set of transformation equations are required to   place the VVV JHKs photometry onto the 2MASS   system. The impetus for this work is to develop those transformations   via a comparison of 2MASS targets in 152 VVV fields sampling the   Galactic disk. The transformation coefficients derived exhibit a   reliance on variables such as extinction. The transformed data were   subsequently employed to establish a mean reddening law of EJ&#8722;H\/EH&#8722;Ks&nbsp;=&nbsp;2.13&nbsp;±&nbsp;0.04,   which is the most precise determination to date and merely emphasizes   the pertinence of the VVV data for determining such important   parameters.","palabras_clave":"GALAXY DISK|GALAXY STELLAR CONTENT|GALAXY STRUCTURE|INFRARED STARS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63116,"idioma_id":4,"titulo":"Rotatable anisotropy of epitaxial Fe$_{1-x}$Ga$_{x}$ thin films","resumen":"We show by a combined magnetic force microscopy and synchrotron radiation spectroscopy study that stripe-like patterned magnetic domains are present in Fe1-xGax thin films. These stripes, whose origin is attributed to an out-of-plane magnetic component, can be rotated by an external magnetic field.","palabras_clave":"-"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63117,"idioma_id":4,"titulo":"oiwa, a female gametophytic mutant impaired in a mitochondrial Mn-SOD, reveals crucial roles for ROS during embryo sac development and fertilization in Arabidopsis thaliana.","resumen":"Reactive oxygen species (ROS) can work as signaling molecules, regulating key aspects of plant development, or as toxic compounds leading to oxidative damage. In this report, we show that control of ROS during megagametogenesis is largely dependent on MSD1, a mitochondrial Mn-superoxide dismutase. WT mature embryo sacs show ROS exclusively in the central cell, which appears to be the main source of ROS before pollination. Accordingly, expression of MSD1 shows a complementary pattern. MSD1 expression is maintained elevated in the egg apparatus at maturity, but is downregulated in the central cell.  oiwa mutants are characterized by high levels of ROS detectable both  in the central cell and in the micropylar cells. Remarkably, egg apparatus cells in oiwa show central cell features, indicating that high levels of ROS result in the expression of central cell-characteristic genes.  Notably, ROS are detected in synergid cells after pollination. This ROS burst depends on stigma pollination but precedes fertilization, suggesting that embryo sacs sense the imminent arrival of pollen tubes and respond generating an oxidative environment. Altogether, we show that ROS play a crucial role during female gametogenesis and fertilization.  MSD1 activity seems critical for maintaining ROS localization and important for embryo sac patterning.","palabras_clave":"embryo sac|female gametophyte|Reactive oxygen species |mitochondrial Mn-SOD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63118,"idioma_id":4,"titulo":"Factors controlling macroalgae assemblages in a Southwest Atlantic coastal lagoon modified by an invading reef forming polychaete","resumen":"In the SW Atlantic Mar Chiquita coastal lagoon (37° 40´S, 57° 23°W, Argentina) the invasive reef building polychaete Ficopomatus enigmaticus can directly and indirectly change the composition and abundance of primary producers by providing habitat to some species and excluding others. The magnitude of these effects may be regulated by consumers and nutrients availability. Here we evaluate the relative importance of consumers (large consumers: birds, fish and crabs; small consumers: amphipods, hydrobiid snails and crabs) and nutrients in controlling macroalgae development on the reefs and on the sediment in areas without reefs. Experiments showed that large consumers positively affect green macroalgae development in the sediment of areas without reefs, probably by enhancing availability of bivalve shells for macroalgae attachment, but had no effects on the development of red and green macroalgae on the reefs. No effect of nutrient addition on macroalgal biomass was detected. Small consumers, however, negatively affected the development of the green alga Cladophora sp., probably by direct consumption, and had a positive effect on the biomass of the red alga Polysiphonia subtilissima. These interactions would determine the abundance of macroalgae, the state of algal mats (e.g., thallus damage) and the spatial distribution (e.g., macroalgae patchiness). Consequently, the effect of an invader ecosystem engineer in the composition of species assemblages may be mediated by other community structuring forces, which in turn may have different effects according their interaction with the invader.","palabras_clave":"biological invasions|macroalgae|consumers|nutrient addition|coastal lagoon"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63119,"idioma_id":4,"titulo":": Freeze drying encapsulation of red wine polyphenols in an amorphous matrix of maltodextrin.","resumen":"...","palabras_clave":"POLYPHENOLS|RED WINE|ENCAPSULATION|FREEZEDRYING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63120,"idioma_id":4,"titulo":"Glyphosate reduces spore viability and root colonization of arbuscular mycorrhizal fungi","resumen":"    Glyphosate  is the most widely used herbicide in the world, but its effects on non-target  organisms, such as arbuscular mycorrhizal fungi (AMF), are unclear. No studies  have been found that made reference to effects of glyphosate on AMF spore  viability despite its importance as a source of propagules for the perpetuation  and spread of AMF in the system. The objective of this study was to evaluate  the effect of glyphosate application on AMF spore viability, and their ability  to colonize roots. Soil samples were collected from a grassland area located in  the Flooding Pampa region (Argentina).  We evaluated three herbicide rates: 0, 0.26 and 1× recommended field rate, 10  and 30 days after application. Part of the soil from each tray was used to  estimate the spore viability, and the remainder was used as substrate for  growing Lolium multiflorum Lam. One month after sowing, total root colonization  and percentage of arbuscules and vesicles were determined. The spore viability  in herbicide untreated soils was between 5.8- and 7.7-fold higher than in  treated soils. This reduction was detected even when the lower rate was  applied. Root colonization was significantly lower in plants grown in  glyphosate treated soil than in untreated ones. A decrease in arbuscular  colonization (but not in vesicles) was found in plants grown in soils treated  with the highest herbicide rate. That would indicate that symbiosis  functionality was affected, given that arbuscules are the main site for  host\u2013fungus nutrient exchange. The results indicate that soil residence time of  glyphosate and\/or its degradation products was enough to reduce AMF spore viability  and their ability to colonize roots. This decrease in propagules viability may affect  plant diversity, taking into account the different degrees of mycorrhizal dependency  between plant species that may coexist in grassland communities.  ","palabras_clave":"NON TARGET ORGANISMS|GRASSLAND|LOLIUM MULTIFLORUM|AMF PROPAGULES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63121,"idioma_id":4,"titulo":"Involvement of the ANGPTs\/Tie-2 system in Ovarian Hyperstimulation Syndrome (OHSS)","resumen":"Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with ovarian stimulation. OHSS   features are ovarian enlargement with fluid shifting to the third   space. Disturbances in the vasculature are considered the main changes   that lead to OHSS.   Our aim was to analyze the levels of angiopoietins 1 and 2 (ANGPT1 and   2) and their soluble and membrane receptors (s\/mTie-2) in follicular   fluid (FF) and in granulosa-lutein cells culture (GLCs) from women at   risk of developing OHSS. We also evaluated the effect of ANGPT1 on endothelial cell migration. In ovaries from an OHSS   rat model, we analyzed the protein concentration of ANGPTs, their   mTie-2 receptor, and platelet-derived growth factor PDGF-B, -D and   PDGFR-&#946;. ANGPT1 levels were increased in both FF and GLCs from women at   risk of OHSS. Incubation of these FF with an ANGPT1 neutralizing antibody decreased endothelial cell migration. In the ovaries of OHSS   rat model, mTie-2 protein levels increased and PDGF-B and -D decreased.   In summary, these results suggest that ANGPT1 could be another mediator   in the development of OHSS.","palabras_clave":"OVARY|ANGIOGENESIS|ANGIOPOIETINS|OHSS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63122,"idioma_id":4,"titulo":"Further evidence of widespread Permian remagnetization in the North Patagonian massif, Argentina","resumen":"A paleomagnetic study on accurately dated (472?476 Ma) Early Ordovician undeformed granitoids exposed in the northeastern margin of the North Patagonian massif (41.5°S, 65.0°W) was carried out, with the main goal of comparing the Early Ordovician paleogeographic position of Patagonia relative to Gondwana. About one hundred specimens were processed, from ten sites on the granitoids plus two sites on the quartzites and sandstones of the unconformably overlying Early Devonian Sierra Grande Formation. Seven out of the twelve sites, all in the granitoids, provided consistent directions of a characteristic remanent magnetization. Structural correction of paleomagnetic data from plutons could only be applied at a few sites, from bedding attitudes of overlying Devonian or Tertiary sedimentary rocks. The paleomagnetic study failed to recover the primary remanence. Thermal demagnetization isolated a reversed magnetic component with high unblocking temperatures. Magnetic properties and petrographic analyses suggest that remagnetization is carried by secondary magnetic minerals that grew from circulating high temperature fluids inferred from the pervasive to localized hydrothermal alteration. This led to the formation of magnetite and\/or hematite after biotite. A paleomagnetic pole (PP) was computed from the mean site directions. The position of this PP (011°E, 65.0°S; A95 = 12°, K = 24.5) on the apparent polar wander path of South America suggests that this magnetization was probably acquired during the Early Permian. Syntectonic remagnetization during Permian times has been reported for sandstones of the lower member of the Devonian Sierra Grande Formation at nearby outcrops. Our data suggest that the remagnetization was pervasive and affected the Ordovician granitoids as well. Many South American Permian paleomagnetic poles have been derived from syn- to postectonic magnetizations along the southwestern Gondwana margin, from the Argentine Precordillera to the Sierras Australes belt and related to the ca. 290?270 Ma San Rafael tectonic phase. Slightly younger remagnetizations have been found affecting sedimentary rocks exposed in the foreland both in Argentina and Uruguay, suggesting a migration of the remagnetization front towards the North-Northeast. The similarity of the position of our new remagnetized pole from the North Patagonian massif to those from units in southern Gondwana confirms that Patagonia was already part of Gondwana by the Early Permian. Whether the remagnetization of the Ordovician granitoids is associated to the widespread and well dated in 290?280 Ma magmatism that affected the northern Patagonia and a major part of southwestern Gondwana during the Permian or can be attributed to tectonic processes associated to the collision of Patagonia, or post-collision adjustment of minor plates, is discussed.","palabras_clave":"PATAGONIA|GONDWANA|PANGEA|PALEOZOIC|PALEOMAGNETISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63123,"idioma_id":5,"titulo":"COMPOSICIÓN QUÍMICA DE BIOTITA Y HORNBLENDA Y EDADES DE ENFRIAMIENTO COMO INDICADORES DE LAS CONDICIONES DE EMPLAZAMIENTO DEL COMPLEJO PLUTÓNICO LA ESPERANZA (PÉRMICO SUPERIOR), MACIZO NORPATAGÓNICO","resumen":"El Complejo Plutónico La Esperanza está situado en el sector norte del Macizo   Norpatagónico y es parte del Cinturón magmático norte Paleozoico superior de la Patagonia.  Dentro de este complejo se agrupan mayormente granodioritas y monzogranitos biotítico35  anfibólicos (Granodiorita Prieto) o biotíticos. (Granito Donosa), siendo 273Ma la edad de   cristalización de los primeros. En este trabajo se utilizan las composiciones minerales de   biotitas y anfíboles para describir la naturaleza del magma y estimar la presión y temperatura  38 del emplazamiento de estos cuerpos plutónicos. En base a la química de las biotitas, el   Complejo Plutónico La Esperanza presenta una signatura magmática calcoalcalina con  tendencia a la peraluminosidad, la cual puede ser asociada a ambientes transicionales orogénico- anorogénico. Las presiones de emplazamiento calculadas para la Granodiorita  Prieto oscilan entre 0.6 y 2.0 kbar registrándose un aumento de la presión en la facies de granitoides inequigranulares aflorante hacia el sector este de Estancia La Esperanza. El  termómetro hornblenda-plagioclasa indica que este cuerpo se emplazó a una temperatura de entre 630-790ºC en condiciones de alta fugacidad de oxígeno. Estos resultados, en combinación con edades de enfriamiento, indican un emplazamiento somero en un ambiente   con un régimen termal elevado que habría permanecido en actividad desde Pérmico medio al  menos hasta el límite permotriásico.","palabras_clave":"Complejo Plutónico La Esperanza|Biotita|Hornblenda|Pérmico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63124,"idioma_id":4,"titulo":"Targeted Delivery of Immunotoxin by Antibody to Ganglioside GD3: A Novel Drug Delivery Route for Tumor Cells","resumen":"Gangliosides are sialic acid-containing glycolipids expressed on plasma membranes from nearly all vertebrate cells. The expression of ganglioside GD3, which plays essential roles in normal brain development, decreases in adults but is up regulated in neuroectodermal and epithelial derived cancers. R24 antibody, directed against ganglioside GD3, is a validated tumor target which is specifically endocytosed and accumulated in endosomes. Here, we exploit the internalization feature of the R24 antibody for the selective delivery of saporin, a ribosome-inactivating protein, to GD3-expressing cells [human (SK-Mel-28) and mouse (B16) melanoma cells and Chinese hamster ovary (CHO)-K1 cells]. This immunotoxin showed a specific cytotoxicity on tumor cells grew on 2D monolayers, which was further evident by the lack of any effect on GD3-negative cells. To estimate the potential antitumor activity of R24-saporin complex, we also evaluated the effect of the immunotoxin on the clonogenic growth of SK-Mel-28 and CHO-K1GD3+ cells cultured in attachment-free conditions. A drastic growth inhibition (.80\u201390%) of the cell colonies was reached after 3 days of immunotoxin treatment. By the contrary, colonies continue to growth at the same concentration of the immuntoxin, but in the absence of R24 antibody, or in the absence of both immunotoxin and R24, undoubtedly indicating the specificity of the effect observed. Thus, the ganglioside GD3 emerge as a novel and attractive class of cell surface molecule for targeted delivery of cytotoxic agents and, therefore, provides a rationale for future therapeutic intervention in cancer.","palabras_clave":"GANGLIOSIDE|IMMUNOTOXIN|CANCER CELLS|DRUG DELIVERY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63125,"idioma_id":4,"titulo":"Stability of Individual Phenolic Compounds and Antioxidant Activity During Storage of a Red Wine Powder","resumen":"The addition of maltodextrin DE 10 to red wine(Cabernet Sauvignon) followed by freeze-drying allowed toobtain a free-flowing (dealcoholized) wine powder (WP) havinga phenolic concentration about 3.6 times higher than theoriginal liquid red wine. The powder, having a water activity(aw) of 0.053 and 0.330 was stored at 28 °C and 38 °C and thecontent of ten different phenolic compounds was determinedby HPLC. Caftaric acid, quercetin 3-glucoside, caffeic acid,gallic acid and resveratrol contents in the WP stored at 28 °Cand 38 °C, remained almost constant during 70 days ofstorage, while epicatechin gallate, catechin, malvidin 3-Gand epicatechin showed slight losses (about 15?25 %) duringstorage. On the contrary, epigallocatechin experienced astrong loss of concentration (around 61% loss) during storageat the same conditions. A moderate decrease of antioxidantactivity, determined by free radical scavenging capacity of the2,2-diphenyl-1-picrylhydrazyl free radical (DPPH*) and ferricreducing antioxidant power (FRAP) was observed alongstorage. This decrease was more important at higher temperature(38 °C) and higher aw (0.33). These results would allowthe feasibility of using this WP as a healthy ingredient inalcohol-free powder drinks.","palabras_clave":"WINE POWDER|DEALCOHOLIZED|POLYPHENOLS|ANTIOXIDANT CAPACITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63126,"idioma_id":4,"titulo":"Adding genotypic differences in reproductive partitioning and grain set efficiency for estimating sorghumk grain number.","resumen":"Current sorghum crop growth models predict grain number using a calculated plant growth rate around flowering and a genotype-dependent parameter that describes the relationship between both traits. Few values of this parameter have been reported; being similar among triple-dwarf and single-dwarf sorghum genotypes. This approach narrows genotypic differences in grain number determination mostly to differences in traits affecting biomass production. Relevant traits like biomass partitioning to reproductive structures and grain set efficiency are not specifically considered, but both vary across genotypes and could improve grain number estimations. In the present study we first explored variation for these traits (CGR, crop growth rate around flowering; PR, biomass partitioning to reproductive structures during this period; EG, grain set per unit of accumulated reproductive biomass) for a set a sorghum commercial hybrids and inbred lines growing at different conditions. Later, we used a second set of experiments to test if considering genotype specific PR and EG improved estimates of grain number when compared to the current equation used in crop simulation models. &nbsp;&nbsp;               Grain number variations (14 to 63 x103 grains m-2) due to genotype and environment were a consequence of significant differences (p&lt;0.05) in all analysed traits (CGR, PR and EG). Biomass partitioning and grain set per unit of accumulated reproductive biomass showed consistent genotypic differences (p&lt;0.001), however they also showed significant environment or genotype x environment effects. When these specific genotypic parameters dealing with biomass partitioning and grain set efficiency were used for estimating grain number at other non-related experiments the accuracy of the predictions improved (r2=0.47, p&lt;0.05, RMSE=7029 grains m-2) when compared to the general model using a constant parameter for most genotypes (r2=0.14, p&lt;0.28, RMSE=12630 grains m-2) or a calculated parameter for each genotype (r2=0.38, p&lt;0.10, RMSE=8919 grains m-2). We proposed these traits (PR and EG) need to be considered and included in sorghum crop growth models, as they help predict grain number performance of different genotypes at different growth environments.","palabras_clave":"sorghum|grain number|kernel set"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63127,"idioma_id":4,"titulo":"Uni- and Biaxial Impact Behavior of Double-Gated Nanoclay-Reinforced Polypropylene Injection Moldings","resumen":"Polypopylene\/nanoclay three-dimensional parts were produced without intermediate steps by direct injection molding to explore the influence of flow features and nanoclay incorporation in their impact performance.  The nanocomposite was obtained by direct compounding of commercial PP with nanoclay masterbatch. The as-molded morphology was analyzed by X-ray and TEM analyses in terms of skin-core structure and nanoclay particle dispersion. The nanoclay particles induced the reduction of b-form spherulites, a known toughener. The impact behavior was assessed in tensile and biaxial modes. The PP nanocomposite molding toughness was practically unaffected by the processing melt temperature and flow rate. Conversely the nanoclay presence is influent in the impact performance.  Under biaxial stress impact, the regions close to weld lines are tougher than the bulk and the fracture develops with main crack paths along the flow direction and the weld line. Cracking along the weld line results from less macromolecular interpenetration and chain entanglement, and unfavorable nanoparticle orientation. It seems that a failure mechanism which involves nanoclay delamination and multiple matrix crazing explains the toughening of PP in the directions where the nanoparticle orientation with respect to loading is adequate.","palabras_clave":"POLYPROPYLENE|INJECTION MOULDING|IMPACT BEHAVIOUR|NANOINDENTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63128,"idioma_id":5,"titulo":"No todo da lo mismo: de la comparación al distanciamiento. El caso de igual","resumen":"Este artículo intenta ofrecer una respuesta a la pregunta acerca de cuáles son las propiedades funcionales y semánticas que habilitan al ítem 'igual' a desencadenar tanto la lectura de posibilidad como la de distanciamiento pasando por la concesión, a partir del reconocimiento de un origen común para todos los empleos de igual como marcador discursivo: el de adverbio de comparación, que deviene a su vez del adjetivo comparativo, y que comporta una cuantificación de indiferencia. El recorrido supone un proceso de gramaticalización (Hopper y Traugott 1993; Company Company 2004, 2008; Diewal 2011), en el que las orientaciones argumentativas que se desprenden del contenido cuantificado juegan un papel determinante en la capacidad del ítem igual para convertirse en un marcador discursivo, externo a la predicación.","palabras_clave":"IGUAL|COMPARACIÓN|DISTANCIAMIENTO|POSIBILIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63129,"idioma_id":4,"titulo":"Nanofiltration of partial oxidation products and copper from catalyzed wet peroxidation of phenol","resumen":"The aim of this work is to study the combination of Catalytic Wet Peroxide Oxidation (CWPO) and nanofiltration processes in the treatment of phenol solutions. A home-made Cu-chitosan\/Al2O3 catalyst was applied for this purpose. The effluent from CWPO usually contains some metal leached from the catalyst in addition to latter oxidation intermediates (short chain organic acids). Downstream nanofiltration is used to retain the metal leached from the catalyst, which would result in secondary pollution and negatively impact on a possible subsequent biological treatment step. The CWPO was performed in a batch recycle trickle bed reactor packed with the catalyst. The final distribution of intermediates was identified by HPLC. Cross flow filtration experiments using either model solutions containing phenol and the main intermediates (succinic, malonic, maleic and oxalic acid), with and without Cu(II) or the effluent of the CWPO reaction were performed using a NF90 membrane. When all intermediates were combined, the rejection of copper reached over 95% while an acceptable maximum of 10% of permeate flux loss, compared with the initial flux, was maintained. Nanofiltration of a real CWPO effluent gave a higher copper recovery of 97%. Thus, the permeate toxicity was highly reduced. In addition, after 4 h of filtration, only a 5% of flux loss was observed, which guarantees a stable filtration for days.","palabras_clave":"CWPO|PHENOL OXIDATION|COPPER|NANOFILTRATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63130,"idioma_id":4,"titulo":"Delivery of recombinant vaccines against bovine herpesvirus type 1 and Babesia bovis to mice using liposomes derived from egg yolk lipids","resumen":"Liposomes prepared from total egg yolk lipid extracts were used to deliver experimental DNA vaccines to mice consisting of pCI-neo plasmids encoding bovine herpesvirus type 1 (BoHV-1) gD or Babesia bovis MSA-2c. A significantly higher proportion of mice in the B. bovis MSA-2c group, but not those in the BoHV-1 gD group, developed detectable immunoglobulin G responses when vaccinated with liposome encapsulated DNA in comparison with mice vaccinated with naked DNA. In both groups, antibody titres were similar between mice vaccinated with liposome encapsulated DNA and naked DNA.","palabras_clave":"DNA vaccine|Egg yolk |liposome encapsulation|Bovine herpesvirus type 1 gD|Babesia bovis MSA-2c|Mice"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63131,"idioma_id":4,"titulo":"Nanostructured Ce1-xZrxO2 solid solutions produced by mechanochemical processing","resumen":"The nanostructured Ce1-xZrxO2 solid solutions (x&lt;0.2) have been successfully synthesized from CeCl3-ZrCl4-NaOH mixtures by mechanochemical processing as a gradual transformation, involving in-situ CeO2 and amorphous ZrO2 formation as intermediates. Solid solutions type-Ce1-xZrxO2 along with NaCl as diluent were obtained at different milling times, with a final composition of Ce0.8Zr0.2O2 after 5 h and 15 h under high and low energetic milling conditions, respectively. The NaCl formed during the mechanochemical reaction, which is eliminated by washing after calcination of the as-milled sample, allows to obtain a Ce0.8Zr0.2O2 solid-solution with high surface area and nanometric grains. The nanostructured Ce0.8Zr0.2O2 solid-solution shows good thermal stability after prolonged heating at 600°C. However, the addition of an extra amount of diluent during mechanochemical reaction evidences a detrimental effect, avoiding the formation of solid solution or deteriorating the textural\/ microstructuctural characteristics of the obtained Ce1-xZrxO2 solid-solution. Removal of NaCl previous to calcination improves notably the textural\/microstructural characteristics of the Ce0.8Zr0.2O2 solid-solution.","palabras_clave":"CERIA|CATALYST|MECHANOCHEMISTRY|NANOSTRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63132,"idioma_id":4,"titulo":"Effective synthesis of Mg2CoH5 by reactive mechanical milling and its hydrogen sorption behavior after cycling","resumen":"Mg2CoH5 was synthesized by reactive mechanical milling (RMM) under hydrogen atmosphere (0.5 MPa) from 2MgH2-Co and 3MgH2-Co mixtures, with a yield >80%. The microstructure, structure and thermal behavior of the phases formed during the processing were investigated by transmission electron microscopy, X-ray diffraction and differential scanning calorimetry. Kinetic properties of the reaction with hydrogen of the 2MgH2-Co and 3MgH2-Co mixtures after RMM were evaluated using modified Sieverts-type equipment. The 3MgH2-Co mixture showed better properties for storage applications, with its highest rate of hydrogen absorption and desorption at 300 °C, its storage capacity of about 3.7 wt% in less than 100 s, and good stability after cycling. Although the starting material presents Mg2CoH5 as majority phase, the cycling leads to disproportion between Mg and Co. We obtained a mixture of Mg2CoH5, Mg6Co2H11 and MgH2 hydrides, as well as other phases such as Co and\/or Mg, depending on experimental conditions.","palabras_clave":"HYDROGEN|STORAGE|HYDRIDES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63133,"idioma_id":4,"titulo":"Low-dimensional magnetic systems in nanopore arrays","resumen":"FeCo nanowires 20 nm diameter are synthesized by AC electrodeposition using AAO membranes as templates. The hysteresis andstructural properties of the as deposited nanowire arrays are characterized. The orientation dependence of the coercive field measuredin the array is compared with the predictions of a magnetization reversalmodel involving the nucleation and propagation of a transversewall. The model is promissory because it predicts the experimental curve shape quite well.","palabras_clave":"ALUMINA|NANOSTRUCTURE|NANOWIRES|MEMBRANE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63134,"idioma_id":4,"titulo":"Validation of a Galerkin technique on a boundary integral equation for creeping flow around a torus","resumen":"A validation of the numerical solution for the steady and axisymmetric creeping flow around a three-dimensional torus is presented. This solution is obtained by means of the Boundary Element Method. Both, a Galerkin weighting technique and collocation to the centroid of the elements are employed. The curve of the viscous drag force as a function of the diameter of the torus relative to its thickness is compared against a semi-analytical solution and laboratory experimental measurements taken from the literature. The semi-analytical solution, as it is known for this kind of geometry, involves the Legendre functions of first and second kind of order one and semi-integer degrees, also called toroidal harmonics.","palabras_clave":"CREEPING FLOW|STEADY FLOW|BOUNDARY ELEMENT METHOD|COLLOCATION TECHNIQUE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63135,"idioma_id":4,"titulo":"Nitric oxide as a key component in hormone-regulated processes","resumen":"Nitric oxide (NO) is a small gaseous molecule,  with a free radical nature that allows it to participate in a  wide spectrum of biologically important reactions. NO is  an endogenous product in plants, where different biosynthetic  pathways have been proposed. First known in animals  as a signaling molecule in cardiovascular and nervous  systems, it has turned up to be an essential component for a  wide variety of hormone-regulated processes in plants.  Adaptation of plants to a changing environment involves a  panoply of processes, which include the control of CO2  fixation and water loss through stomatal closure, rearrangements  of root architecture as well as growth restriction.  The regulation of these processes requires the  concerted action of several phytohormones, as well as the  participation of the ubiquitous molecule NO. This review  analyzes the role of NO in relation to the signaling pathways involved in stomatal movement, plant growth  and senescence, in the frame of its interaction with abscisic  acid, auxins, gibberellins, and ethylene.","palabras_clave":"abscisic acid|auxins|ethylene|gibberellins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63136,"idioma_id":4,"titulo":"New structures for goat corrals to control peridomestic populations of Triatoma infestans (Hemiptera: Reduviidae) in the Gran Chaco of Argentina","resumen":"Goat production is an important economic activities for rural communities in the GranChaco of Argentina. Goat corrals are important for the survival of T. infestans peridomestic populations. This study evaluated the impact of the modification of the traditional structure of the goat corral on T. infestans populations and on goat productivity in the region of Los Llanos (La Rioja). Thirty nine experimental corrals were constructed and 57 traditional corrals were kept as controls. We evaluated the infestation of the control and experimental corrals during five years after the structures construction. The results showed that the new structures enhanced T. infestans infestation detection, decreased the abundance of this species and contributed to a better control and monitoring of their populations by vector control programs using pyrethroid insecticides, because of the increased visibility of the insects and a better performance of the suspension concentrate formulation of the pyrethroid. The experimental corrals significantly increased goat productivity and represent an element through which local governments can promote the development of rural communities. Acceptability and adoption of the new corrals by the owners was high, as the enclosures offer a better protection for the goats, higher growth of kids and facilitates the herd handling.T. infestans peridomestic populations. This study evaluated the impact of the modification of the traditional structure of the goat corral on T. infestans populations and on goat productivity in the region of Los Llanos (La Rioja). Thirty nine experimental corrals were constructed and 57 traditional corrals were kept as controls. We evaluated the infestation of the control and experimental corrals during five years after the structures construction. The results showed that the new structures enhanced T. infestans infestation detection, decreased the abundance of this species and contributed to a better control and monitoring of their populations by vector control programs using pyrethroid insecticides, because of the increased visibility of the insects and a better performance of the suspension concentrate formulation of the pyrethroid. The experimental corrals significantly increased goat productivity and represent an element through which local governments can promote the development of rural communities. Acceptability and adoption of the new corrals by the owners was high, as the enclosures offer a better protection for the goats, higher growth of kids and facilitates the herd handling.","palabras_clave":"Triatoma infestans|vector control|goal productivity|Chagas disease"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63137,"idioma_id":5,"titulo":"Produccion de bovinos bi transgenicos y clonados","resumen":"La modificación de genomas para la obtención de animales transgénicos por distintas técnicas ofrece una nueva alternativa para la producción de proteínas recombinantes (?animal pharming?). La principal aplicación ha sido la obtención de proteínas humanas para uso tanto medicinal (farmacéutico) como alimenticio (nutricéutico), producidas en leche de animales de granja transgénicos. Los objetivos de este trabajo fueron: obtener a través de métodos de Ingeniería Genética un vector capaz de introducir en el genoma de una línea celular dos genes humanos (lactoferrina y lisozima humanas) bajo la acción de un solo promotor y, utilizando estas células transformadas y mediante la técnica de clonación por transferencia nuclear obtener embriones, preñeces, gestaciones y un individuo bitransgénico que exprese a nivel de glándula mamaria las dos proteínas correspondientes. Se utilizó un vector pIRES2-EGFP (Clontech inc, USA), al cual se le eliminó el promotor de CMV y se adicionó el promotor de &#946;-caseína caprina y el gen de lisozima y lactoferrina humanas. Se efectuaron estudios preliminares transfectando células mamarias murinas con el vector de expresión desarrollado en este trabajo, encontrándose ambas proteínas en los cultivo celulares luego de inducir la expresión por métodos hormonales. Al cumplir un año y un mes de vida, la ternera fue inducida artificialmente a la lactancia verificándose en su leche, mediante la técnica de Western Blot, las proteínas lisozima y lactoferrina humana. Como resultado de estos experimentos se logró desarrollar un vector funcional capaz de introducir ambos genes humanos en el genoma bovino, manteniéndose los mismos durante el desarrollo embrionario, gestación y luego del nacimiento, logrando expresar las proteínas correspondientes a nivel de la glándula mamaria. Esto constituye un logro sin antecedentes a nivel mundial, debido a que no se dispone de informes sobre bovinos bitransgénicos","palabras_clave":"SCNT|TRANSGENESIS|VECTOR|IRES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63138,"idioma_id":4,"titulo":"Probability mapping images in dynamic speckle classification","resumen":"We propose the use of a learning procedure to identify regions of similar dynamics in speckle imagesequences that includes more than one descriptor. This procedure is based on the application of a naïveBayes statistical classifier comprising the use of several descriptors. The class frontiers can be depicted sothat the proportion of identified regions may be measured. To demonstrate the results, assembly of anRGB image, where each plane (R, G, and B) is associated with a particular region (class), was labeledaccording to its biospeckle dynamics. A high brightness in one color means a high probability of the pixelbelonging to the corresponding class, and vice versa.","palabras_clave":"Dynamic speckle|Neural network|Naive Bayes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63139,"idioma_id":4,"titulo":"Checklist of the Argentine Agaricales 5. Agaricaceae","resumen":"ABSTRACT?A checklist of species belonging to the family Agaricaceae was made for Argentina. The list included all species published up to 2011. Thirteen genera and 171 species were recorded.","palabras_clave":"BIODIVERSITY|NOMENCLATURE|SOUTH AMERICA|TAXONOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63140,"idioma_id":4,"titulo":"Hydrogen recovery from the purge stream of an HDA process using the concept of Mass Exchange Networks","resumen":"In this paper we use the concept of Mass Exchange Networks to design the hydrogen recoveryfrom the purge stream of an HDA Process by implementing a recently proposed counter current gas permeation equipment to exchange hydrogen between the purge and the toluene feed to the process. This design would correspond to the final design refinement step in the Douglas [1,2] hierarchical process design procedure, proposed by Fischer and Iribarren [3]. The goal of this design is recovering part of the hydrogen available in the purge stream, and results in a process alternative different from other flow sheet recently proposed by Bouton and Luyben [4], also resorting to gas permeation membrane units, but in a traditional arrangement. Two different types of available zeolite ceramic membranes were studied, of different permeability and selectivity. The here proposed mass exchange design recovers a similar amount of hydrogen as the process alternative proposed by Bouton and Luyben [4] who use a less expensive type of polymeric membrane, but need a compressor to recycle the permeate stream because they use transmembrane pressure as the driving force. The here proposed design at actual cost of zeolite membranes allows an 153.9% increase of the Net Annual Savings with respect to the pressure driven membrane system when using the less selective ceramic membrane, while this figure descents to a 32.61% when using the most selective (which is also the most expensive alternative).","palabras_clave":"HDA|HYDROGEN|MASS EXCHANGE|INTEGRATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63141,"idioma_id":4,"titulo":"Suitable regions for assessing long term trends in lightning activity","resumen":"The efficiency and stability of global lightning data are not yet sufficient to provide accurate estimatesof long term trends in lightning activity. In contrast, regional lightning networks are generally bothefficient and stable. Regions with a low level of interannual variability are well suited to theidentification of trends in lightning activity. Satellite lightning data are used to identify countries inSouth America which display only mild interannual variability. These countries are candidates forregional studies of long term trends in lightning activity. These trends can be linked to climate change.","palabras_clave":"Lightning|Thunderstorms|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63142,"idioma_id":4,"titulo":"Spatial and time distribution of the flash rate over tropical Africa","resumen":"The lightning ash rate over tropical Africa was analyzed using lightning data of two independent lightning detection systems, the World Wide Lightning Location Network and the Lightning Imaging Sensor. Spatial and time distributions of lightning activity were studied using different spatial and temporal scales. Results show a very localized high-lightning activity center on the west side of the Albertine Rift Mountain, located east of the Congo Basin, and substantial lightning activity in the center of the region. Both centers show low variations on the lightning activity throughout the year and a marked diurnal cycle with a maximum lightning activ ty in the local afternoon. A secondary lightning activity center was found over Lake Victoria, which has an annual variation similar to that of the Congo Basin lightning activity centers but does not show a marked diurnal cycle. The lightning activities observed seem to be strongly in&#64258;uenced by topography and are in agreement with the small temperature ranges and with the rainfall observed in the region.","palabras_clave":"Tropical |Africa |lightning"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63143,"idioma_id":5,"titulo":"DIVERSIDAD EN EL INSTRUMENTAL LÍTICO Y USO DEL ESPACIO  DURANTE EL HOLOCENO MEDIO Y  TARDÍO EN LA COSTA NORTE DEL GOLFO SAN MATÍAS (RÍO NEGRO, ARGENTINA)","resumen":"La costa norte del golfo San Matías presenta una gran cantidad de loci arqueológicos, asignables a dos períodos diferentes del consumo de recursos marinos correspondientes al Holoceno medio-tardío y tardío final. Los conjuntos artefactuales líticos y las materias primas usadas en cada uno de estos bloques temporales presentan diferencias de índole cronológica y geográfica que son abordadas en el presente trabajo. A partir del análisis estadístico de los conjuntos recuperados, se establecen diferencias significativas en relación con la distribución de las clases de instrumentos en los bloques temporales definidos. Los resultados sugieren que la diversidad estaría estructurada cronológicamente más que en forma espacial, lo cual resulta acorde con el modelo de uso de los recursos propuesto previamente (Favier Dubois et al. 2009). Respecto del uso de materias primas líticas, se observan diferencias que tendrían que ver con cambios en las estrategias de aprovisionamiento a lo largo del tiempo","palabras_clave":"Holoceno medio y tardío|diversidad y riqueza artefactual|materias primas líticas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63144,"idioma_id":4,"titulo":"Quantum control of a model qubit based on a multi-layered quantum dot","resumen":"In this work we present a model qubit whose basis states are eigenstates of a multi-layered quantum dot. We show that the proper design of the quantum dot results in qubit states that have excellent dynamical properties when a time-dependent driving is applied to it. In particular, it is shown that a simple sinusoidal driving is sufficient to obtain good quality Rabi oscillations between the qubit states. Moreover, the switching between states can be performed with very low leakage, even under off-resonance conditions. In this sense, the quantum control of the qubit is robust under some perturbations and achieved with simple means. More precisely, in this article, we propose a device that is within the reach of current semiconductor technology, a ZnS\/CdSe\/ZnS\/CdSe\/ZnS layered quantum dot. In addition, in order to clarify, we show a more general and theoretical model, which we believe is helpful in order to search the ideal experimental device.","palabras_clave":"quantum dots|control|qubit"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63145,"idioma_id":4,"titulo":"Labile and recalcitrant carbon in crop residue and soil under no-till practices in central region of Argentina","resumen":"Abstract We evaluated the effects of the amount and type of C compounds (labile\/recalcitrant) of crop residues on the soil organic matter (SOM) components under no-tillage practices in semiarid region of Argentina, with the aim of establishing criteria for sustainable agriculture. Five agricultural situations were evaluated: soybean\/wheat rotation; soybean monoculture; soybean\/maize rotation and maize\/wheat\/potato rotations with biannual ploughing. In each situation crop residues and soil samples were collected. In residue samples, biomass (total and components), organic carbon, insoluble fibers, and soluble C compounds were evaluated. In soil samples total SOM, non-humic substances (NHS), humic substances (HS), humic (HA), and fulvic acids (FA) were analyzed. The total biomass and its components differed among situations and were lower in the situation with plough, while the fiber contents were higher in situations that includes wheat crop. Total SOM, NHS, HA content were higher in the plowed situation (57.20 g kg-1, 47.30 g kg-1, 7.80 g kg-1 respectively). We concluded that the total SOM quantity in continuous no-tillage is not affected by the different crop rotations and sequences, but the HA quantity (stable SOM) seems to be favored by the high recalcitrant compounds amount of the crop residues (particularly wheat). For these reason, the following criteria for sustainable agriculture management in semiarid zones can be suggested: a) continuous no-tillage utilization; b) summer crop annual rotations; and c) winter wheat crop instead of bare fallow.","palabras_clave":"Soluble C|Crop residues biomass|Fibers|Humics Substances"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63146,"idioma_id":4,"titulo":"Sustainability\/resilience of soil organic matter components in an Argentinean arid region","resumen":"This short communication presents a novel approach to determining the soil sustainability of productive practices in an Argentinean arid region, using the resilience degree of soil organic matter   components. The study was conducted in four sites of the Arid Chaco region of the Cordoba province: one undisturbed site, two sites with livestock (with total and with selective clearing) and one site   with agriculture. In each site three soil samples were taken and total soil organic matter, fulvic and   humic acids, and non-humic substances were analyzed. Variations of each component (%) between   each productive practice and the undisturbed site were calculated in order to establish the resilience   degree. The livestock soils showed: a) moderate resilience for non-humic substances, b) low resilience   for organic matter and humic acids, and c) no resilience for fulvic acids. The agricultural soils showed:   a) low resilience for total organic matter and non-humic substances, and b) no resilience for fulvic   and humic acids. We conclude that this approach is a powerful tool for establishing management   practices according to each particular situation, allowing improved productivity in arid regions.","palabras_clave":"Arid Chaco|Humic Acids|Clearing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63147,"idioma_id":4,"titulo":"Assessment of antioxidant responses and trace metal accumulation  by digestive gland of ribbed mussel Aulacomya atra atra from Northern Patagonia","resumen":"Seasonal and spatial variability of trace metal concentrations and of a battery of antioxidant parameters were evaluated in digestive gland of the ribbed mussel Aulacomya atra atra. Fe, Al and Cu accumulated in tissue exhibited maximum values in winter, coinciding partially with the highest labile concentrations of Fe and Cu in sediment. Metals, as other pollutants, are known to influence the oxidative status of organisms and antioxidant enzymes have been often proposed as biomarkers of exposure to contaminants. Seasonal variations of trace metals did not appear to influence those of biochemical parameters, which generally showed an opposite trend with higher enzymatic activities in summer when trace metal concentrations were lower. The effects of metals on biomarkers were more evident when mussels from different sites were compared. In particular, organisms from Punta Cuevas (control site) showed higher induction of reactive oxygen species production than those from both considered impacted sites, suggesting the possibility of some biochemical adaptation in organisms or a higher modulation of environmental and physiological factors on antioxidant responses than levels of trace metals. This study, which is the first in the area in this matter, showed that seasonal variations of potential biomarkers should be incorporated into interpretation of long-term biomonitoring studies in this marine coastal ecosystem.","palabras_clave":"Biomarkers|Trace metals|Oxidative stress|Ribbed mussel Aulacomya atra atra"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63148,"idioma_id":4,"titulo":"Distribution and origin of trace metals in sediments of a marine park (Northern San Jorge Gulf) from Argentina","resumen":"The Northern San Jorge Gulf (NSJG) was designated Interjurisdictional   Coastal Marine Park ?Patagonia Austral? in 2008 with the objective of   conserving biodiversity and natural resources. Metals released to the   environment can be accumulated by organisms and can be toxic in some   cases, making it necessary to evaluate their presence and biological   risk. This study examined concentrations of Fe, Mn, Zn, Cu, Cr, Ni, Cd   and Pb in intertidal sediments of the NSJG, and was the first study of   its kind to be conducted in this area. Concentrations of all metals fell   below biological risk levels. Anthropogenic enrichment was only found   for Ni around the Aristizábal lighthouse and was attributed to the   frequent oil spills that impact this particular area.","palabras_clave":"Trace metals|Surface sediments|Coastal Marine Park Patagonia Austral|San Jorge Gulf Basin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63149,"idioma_id":4,"titulo":"Mutations in the EXT1 and EXT2 genes in Spanish patients with multiple osteochondromas","resumen":"Multiple osteochondromas is an autosomal dominant skeletal disorder characterized by the&nbsp; formation of multiple cartilage-capped tumours. Two causal genes have been identified, EXT1 and EXT2, which account for 65% and 30% of cases, respectively. We have undertaken a mutation analysis of the EXT1 and EXT2 genes in 39 unrelated Spanish patients, most of them with moderate phenotype, and looked for genotype-phenotype correlations.Wefound the mutant allele in 37 patients, 29 inEXT1 and 8 inEXT2. Five of the EXT1 mutations were deletions identified by MLPA. Two cases of mosaicism were documented. We detected a lower number of exostoses in patients with missense mutation versus other kinds of mutations. In conclusion, we found a mutation in EXT1 or in EXT2 in 95% of the Spanish patients. Eighteen of the mutations were novel.","palabras_clave":"Multiple osteochondromas|METABOLIC DISORDERS|Bone tumours|EXT genes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63150,"idioma_id":5,"titulo":"Uso de un Sistema Computarizado de Análisis Seminal (CASA)","resumen":"El sistema CASA se introduce en el mercado originalmente para la evaluación de semen humano. Con el paso de los años se ha ido perfeccionando y modernizando, y gracias a que su precio fue descendiendo, ha sido incorporado a otros ámbitos además del de reproducción humana. En la actualidad se utiliza en veterinaria, tanto en la investigación como en la industria, como es el caso de los centros de inseminación artificial.","palabras_clave":"Semen|CASA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63151,"idioma_id":4,"titulo":"Habitat occupancy of the Dusky-legged Guan in the lower delta of the Paraná River, Argentina","resumen":"The Dusky-legged Guan Penelope obscura is the southernmost species of the family Cracidae, reaching its southern distributional limit in the delta of the Paraná River. Habitat loss, together with uncontrolled harvest, has led to local-scale decreases or extirpation of the species, but no quantitative evaluation of habitat preferences has been made. We surveyed Dusky-legged Guans in the Delta del Paraná Biosphere Reserve, Argentina, by motorboat along 543.9 km of nine waterways during both January and July 2009 and used occupancy modelling to estimate habitat and seasonal effects on occupancy. Detectability was 21\u201322% on average and occupancy estimates were highly variable within habitats, but highest in secondary forest, followed by mature plantation, and lowest in occupied residences. There were no significant differences in occupancy or detectability among habitats or seasonally. There was a strong positive effect of length of riparian habitat segments on occupancy and detectability. Habitat management efforts should address increasing the suitability of mature plantation forest for guans by increasing their similarity to native forest in structure and composition. Furthermore, we illustrate that surveys by boat can be logistically effective for surveying cracids associated with riverine habitats and that it is important to account for incomplete detectability since in our case failing to do so would have underestimated occupancy by 78\u201379% on average. Given this, the use of commonly accepted methodologies for surveying cracids that do not account for incomplete detectability should be reconsidered and methodologies that can produce robust, reliable estimates applied.","palabras_clave":"RIVER|SPECIES|CRACIDAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63152,"idioma_id":4,"titulo":"Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions","resumen":"Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and\/or activities of specific synthesizing enzymes.","palabras_clave":"PHOSPHOLIPID|CIRCADIAN RHYTHM|FIBROBLAST|SERUM SHOCK|SYNCHRONIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63153,"idioma_id":4,"titulo":"Enhanced phosphatidylinositol 3-kinase (PI3K)\/Akt signaling has pleiotropic targets in hippocampal neurons exposed to iron-induced oxidative stress","resumen":"The phosphatidylinositol 3-kinase (PI3K)\/Akt pathway is a key component   in synaptic plasticity and neuronal survival. The aim of this work was   to investigate the participation of the PI3K\/Akt pathway and its outcome   on different molecular targets such as glycogen synthase kinase 3&#946;   (GSK3&#946;) and Forkhead box-O (FoxO) transcription factors during mild   oxidative stress triggered by iron overload. The exposure of mouse   hippocampal neurons (HT22) to different concentrations of Fe2+ (25-200 &#956;M) for 24 h led us to define a mild oxidative injury status (50 &#956;M Fe2+)   in which cell morphology showed changes typical of neuronal damage,   with increased lipid peroxidation and cellular oxidant levels but no   alteration of cellular viability. There was a simultaneous increase in   both Akt and GSK3&#946; phosphorylation. Levels of phospho-FoxO3a (inactive   form) increased in the cytosolic fraction of cells treated with iron in a   PI3K-dependent manner. Moreover, PI3K and Akt translocated to the   nucleus in response to oxidative stress. Iron-overloaded cells harboring   a constitutively active form of Akt showed decreased oxidants levels.   Indeed, glutathione (GSH) synthesis under oxidative stress conditions   was regulated by activated Akt. Our results show that activation of the   PI3K\/Akt pathway during iron-induced neurotoxicity regulates multiple   targets such as GSK3&#946;, FoxO transcriptional activity and glutathione   metabolism thus modulating neuronal response to oxidative stress.","palabras_clave":"PI3K|FOXO Transcription factors|oxidative stress|iron"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63154,"idioma_id":4,"titulo":"Specific roles for Group V secretory PLA2 in retinal iron-induced oxidative  stress. Implications for age-related macular degeneration.","resumen":"    Iron  accumulation and oxidative stress are hallmarks of retinas from patients with  age-related macular degeneration (AMD). We have previously demonstrated that  iron-overloaded retinas are a good in  vitro model for the study of retinal degeneration during iron-induced  oxidative stress. In this model we have previously characterized the role of  cytosolic phospholipase A2 (cPLA2) and  calcium-independent isoform (iPLA2). The aim of the present study  was to analyze the implications of Group V secretory PLA2 (sPLA2),  another member of PLA2 family, in cyclooxigenase (COX)-2 and nuclear  factor kappa B (NF-&#954;B) regulation. We found that sPLA2 is localized in  cytosolic fraction in an iron concentration-dependent manner. By immunoprecipitation  (IP) assays we also demonstrated an increased association between Group V sPLA2  and COX-2 in retinas exposed to iron overload. However, COX-2 activity in IP  assays was observed to decrease in spite of the increased protein levels  observed. p65 (RelA) NF-&#954;B levels were increased in nuclear fractions from  retinas exposed to iron. In the presence of ATK (cPLA2 inhibitor)  and YM 26734 (sPLA2 inhibitor), the nuclear localization of both p65  and p50 NF-&#954;B subunits was restored to control levels in retinas exposed to  iron-induced oxidative stress. Membrane repair mechanisms were also analyzed by  studying the participation of acyltransferases in phospholipid remodeling  during retinal oxidation stress. Acidic phospholipids, such as  phosphatidylinositol (PI) and phosphatidylserine (PS), were observed to show an  inhibited acylation profile in retinas exposed to iron while  phosphatidylethanolamine (PE) showed the opposite. The use of PLA2  inhibitors demonstrated that PS is actively deacylated during iron-induced  oxidative stress. Results from the present study suggest that Group V sPLA2  has multiple intracellular targets during iron-induced retinal degeneration and  that the specific role of sPLA2 could be related to inflammatory  responses by its participation in NF-&#954;B and COX-2 regulation.   ","palabras_clave":"RETINA|IRON|PHOSPHOLIPASE A2|MACULAR DEGENERATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63155,"idioma_id":4,"titulo":"Water content effect on the chromatic attributes of dehydrated strawberries during storage, as evaluated by image analysis","resumen":"The changes of chromatic attributes due to different 13 hydration degree,  14 anthocyanins degradation and browning development were studied in dehydrated  15 strawberries. Strawberry slices were freeze-dried, equilibrated at different relative  16 humidities and stored at 45 ºC. The changes in reflectance were analyzed using a computer  17 vision system, employing segmented image analysis. Darkening promoted by browning  18 reactions could be evaluated through the decrease of L* and the increase of b* in the light  19 sections of the samples. The anthocyanin degradation increased as increasing water content.  20 a* values of the reddish sections of the samples correlated with anthocyanin degradation,  21 only at RH values higher than 43%. Light diffusion in the dried material caused a lighter  22 appearance up to 75% relative humidity, while browning reaction and pigment degradation  23 were accelerated at this relative humidity value. These effects were related with the  appearance of mobile water populations in the material, as detected by 124 H-NMR.","palabras_clave":"STRAWBERRY|FREEZE DRIED|IMAGE SEGMENTATION|WATER CONTENT|ANTHOCYANINS|LOW FIELD 1H NMR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63156,"idioma_id":4,"titulo":"Effect of bovine viral diarrhea virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers","resumen":"The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on thereproductive female tract by means of analyzing the ovarian follicular population ofpersistently infected (PI) heifers, and evaluating the performance of oocytes procured formthose heifers in in vitro fertilization procedures.Seven BVDV PI Aberdeen Angus and British crossbred heifers ranging from 18 to36 months of age were spayed and their ovaries used for viral isolation, microscopicexamination, and in vitro fertilization procedures. Bovine Viral Diarrhea Virus wasdetected from the follicular fluid and sera of all PI heifers. Microscopic examination of theovaries from PI heifers showed a significant drop in the number of follicles cortical regions,compared with controls. A comparative analysis of the stages of follicular developmentshowed a significant decrease in the number of primordial and tertiary follicles in thecortical regions of ovaries from PI heifers. Viral antigen was detected by immunohistochemistry,and was widely distributed throughout the ovarian tissues. There weredifferences in the rate of cleavage and embryo development between oocytes obtainedfrom the ovaries of control animals and PI heifers. Furthermore, two developed embryosobtained from oocytes from one of the PI heifers were positive to BVDV, as well as twomedia from in vitro fertilization (IVF) procedures. The results of this study demonstratethat BVDV PI heifers exhibit alterations in follicular population through of the earlyinteraction between the virus and germ cell line affecting directly the mechanismsinvolved in the ontogenesis of the ovary.","palabras_clave":"DIARREA-VIRAL-BOVINA|OVARIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63157,"idioma_id":4,"titulo":"RNA-seq analysis of single bovine blastocysts","resumen":"BackgroundUse of RNA-Seq presents unique benefits in terms of gene expression analysis because of its wide dynamic range and ability to identify functional sequence variants. This technology provides the opportunity to assay the developing embryo, but the paucity of biological material available from individual embryos has made this a challenging prospect.ResultsWe report here the first application of RNA-Seq for the analysis of individual blastocyst gene expression, SNP detection, and characterization of allele specific expression (ASE). RNA was extracted from single bovine blastocysts (n = 5), amplified, and analyzed using high-throughput sequencing. Approximately 38 million sequencing reads were generated per embryo and 9,489 known bovine genes were found to be expressed, with a high correlation of expression levels between samples (r > 0.97). Transcriptomic data was analyzed to identify SNP in expressed genes, and individual SNP were examined to characterize allele specific expression. Expressed biallelic SNP variants with allelic imbalances were observed in 473 SNP, where one allele represented between 65-95% of a variant\u2019s transcripts.ConclusionsThis study represents the first application of RNA-seq technology in single bovine embryos allowing a representation of the embryonic transcriptome and the analysis of transcript sequence variation to describe specific allele expression.","palabras_clave":"RNA Seq|bovine|SNP|blastocyst"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63158,"idioma_id":4,"titulo":"Polymorphic behavior during isothermal crystallization of high stearic high oleic sunflower oil stearins","resumen":"The polymorphic phases of two high stearic high oleic (HSHO) sunflower oil stearins obtained by dry and solvent fractionation of the oil with the aim of use them as trans fat replacers or cocoa butter equivalent were studied by performing in situ isothermal crystallization studies following the appearance of polymorphic forms by X-ray using synchrotron radiation. Thermal behavior, equilibrium and actual isothermal solid fat content and morphology of phases were also analyzed. Three polymorphic forms were observed when samples were crystallized at 10°C\/min to different crystallization temperatures (Tc): &#945;, &#946;\u20192, and &#946;\u20191. The &#61537; form was the first polymorph obtained at all temperatures used and in the opposite way expected, at most crystallization temperatures it did not disappear when &#946;\u20192 or &#946;\u20191 forms appeared. &#946;\u20192 form crystallized below 16 or 23°C for soft and hard stearins, respectively. Above those temperatures, the obtained polymorph was the &#946;\u20191 form. The &#946; polymorphs were not obtained during the times selected for isothermal crystallization. However, &#946;2 form appeared at least after 6 h at Tc while after 48 h of storage at 25°C the &#946;1 polymorph was the main form. &#946;2, the one required for chocolate manufacture, has a very short life and was obtained isolated from &#946;1 by applying cooling\/reheating cycles. The &#946;1 form was the most frequently observed. Therefore, the processing conditions must be carefully controlled to obtain the desired polymorphic form. This study provided the characterization and quantification in real time of polymorphic phases of HSHO stearins, which is very relevant to industrial applications.","palabras_clave":"POLYMORPHISM|MICROSTRUCTURE|THERMAL BEHAVIOR|ISOTHERMAL CRYSTALLIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63159,"idioma_id":4,"titulo":"Lack of support for the association between facial shape and aggression: a reappraisal based on a worldwide population genetics perspective","resumen":"Antisocial and criminal behaviours are multifactorial traits whose interpretation   relies on multiple disciplines. Since these interpretations may have social, moral   and legal implications, a constant review of the evidence is necessary before   any scientific claim is considered as truth. A recent study proposed that men   with wider faces relative to facial height (WHR) are more likely to develop   unethical behaviour mediated by a psychological sense of power. This research   was based on reports suggesting that sexual dimorphism and selection would   be responsible for a correlation between WHR and aggression. Here we show   that 6,243 individuals from 100 modern human populations belonging to a vast   array of genetic and cultural contexts do not display significant amounts of WHR   sexual dimorphism. Sexual dimorphism was indeed more pronounced in other   craniofacial indices, and there was no significant evidence supporting that   populations with higher levels of bellicosity, aggressive behaviour, or powermediated behaviour display greater WHR. Finally, a regression analysis of   WHR on individual?s fitness measures showed no significant correlation   between this facial trait and reproductive success. Our results suggest that   facial attributes are poor predictors of behaviour, or, at least, that sexual   selection was weak enough to leave a signal on patterns of between and within   sex and population facial variation.","palabras_clave":"WIDTH-TO-HEIGHT FACIAL RATIO|SEXUAL DIMORPHISM|HUMAN BEHAVIOUR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63160,"idioma_id":5,"titulo":"Triquinelosis: estudio epidemiológico retrospectivo de casos durante el periodo 1998-2009 en la provincia de Santa Fe. Argentina","resumen":"Objetivos. Analizar las características epidemiológicas, clínicas y de laboratorio a partir de los datos sobre casos y brotes de trichinelosis que se presentaron en la provincia de Santa Fe (Argentina) entre los años 1998 y 2009.   Métodos. A partir de 1519 fichas epidemiológicas de personas que presentaron síntomas y signos compatibles con triquinelosis o que estuvieron asociados epidemiológicamente a casos confirmados de triquinelosis por laboratorio en la provincia de Santa Fe correspondientes al período 1998-2009, se realizó un análisis epidemiológico retrospectivo con base en estadísticas descriptivas y de asociación de variables (clínicas, demográficas, culturales y de laboratorio).   Resultados. El 58,7% de los casos clínicos eran de sexo masculino con un rango de edades entre 11 y 50 años. Los residentes en áreas rurales tuvieron mayor riesgo (OR=1,79) de enfermar que aquellas personas que vivían en las ciudades. El alimento implicado en la totalidad de los casos fue la carne o productos elaborados a base de carne porcina. El 48,66% de los individuos enfermos y confirmados por laboratorio presentaron los signos de mialgia y eosinofilia. El 29,3% de los individuos que presentaron los signos característicos de la enfermedad (mialgia, edema bipalpebral y eosinofilia) fueron confirmados por laboratorio. Se pudo establecer que la región geográfica específica donde se presentaron la mayor proporción de brotes y casos se corresponde con la región donde se concentra la mayor producción de cerdos de la provincia. Los meses en los que se presentan agrupados los casos con mayor frecuencia son los de agosto, setiembre y octubre.   Conclusiones. La situación epidemiológica actual de la trichinelosis en la provincia de Santa Fe (Argentina) indica la necesidad de continuar y profundizar las acciones de gestión y comunicación del riesgo tendientes a erradicar el consumo de carne y subproductos a base de carne porcina sin inspección veterinaria ante y post-mortem.","palabras_clave":"TRIQUINOSIS|EPIDEMIOLOGÍA|CHACINADOS|SALUD PÚBLICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63161,"idioma_id":4,"titulo":"Fire history in the Araucaria araucana forests of Argentina: Human and climate influences","resumen":"Little is known about drivers and trends of historic fire regimes in the Araucaria araucana forests of southwestern Argentina. Fire history in these forests was reconstructed by the analysis of 246 fire-scarred partial cross-sections from this fire-resistant tree collected at 10 sites in Neuque´n, northern Patagonia. Fire chronologies showed an increase in fire occurrence during the nineteenth century and a sharp decrease since the early twentieth century. The creation of Lanín  National Park in 1937, the change in human activities, and the active suppression of wildfires led to a significant increase in mean fire intervals since 1930. In addition to these multidecadal to centennial scale drives of fire frequency, interannual variability in wildfire activity was associated with El Nin˜o\u2013Southern Oscillation. Years of widespread fire are related to negative departures of both Nin˜o 3.4 and Pacific Decadal Oscillation indexes (i.e. La Nin˜a conditions), as well as coincident phases of positive Southern Annular Mode and La Niña events. Temporal variations in the Araucaria fire history in Argentina clearly show the combined effect of human and climate influences on fire regimes.Acomparison with previous fire history studies in the Araucaria forests of Chile reveals substantial differences related to differences in human activities on both sides of the Andes and the earlier implementation of protected areas in Argentina.","palabras_clave":"dendroecology|fire scars|Patagonia|tree rings"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63163,"idioma_id":4,"titulo":"Variation in Mitochondrial NADH Dehydrogenase Subunit 5 and NADH Dehydrogenase Subunit 4 Genes in the Chagas Disease Vector Triatoma infestans (Hemiptera: Reduviidae)","resumen":"Variation in mitochondrial NADH dehydrogenase subunit 5 (ND5) and NADH dehydrogenase subunit 4 (ND4) genes was surveyed in Triatoma infestans from 24 localities of Argentina. The DNA sequence comparisons of 2,183 basepairs of the mitochondrial genome, which include the complete sequence of ND5 (1,712 basepairs) and 401 basepairs of ND4 genes, showed 19 haplotypes determined by 48 variable sites and a nucleotide diversity value of 0.292%. Twenty-six (65%) substitutions were synonymous, and there were 14 (35%) predicted amino acid replacements in ND5. In ND4, 5 (62.5%) substitutions were synonymous and 3 (37.5%) were replacement sites. Samples from six localities studied shared one haplotype and the rest of the localities had different haplotypes. The amplified regions should be useful for population genetic studies.","palabras_clave":"TRIATOMA|CHAGAS|GENES|VARIATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63164,"idioma_id":4,"titulo":"Pheromone-induced morphogenesis improves osmoadaptation capacity by activating \tthe HOG MAPK pathway.","resumen":"Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the ?shmooing? morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.","palabras_clave":"Pheromone-Induced Morphogenesis|HOG MAPK Pathway"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63165,"idioma_id":4,"titulo":"Optimization of Multi-Effect Evaporation desalination plants","resumen":"This paper focuses on the mathematical modeling and optimization of Multi-Effect Evaporation plants (MEE).A simplified and detailed enough model to accurately predict the MEE system performance is presented. Themodel is highly nonlinear and it is based on mass and energy balances which are derived from a superstructure.The superstructure involves several process configurations which have to be simultaneously optimizedto determine the best stream flow-patterns (synthesis), the size of each evaporation effect (sizing) and theoperating conditions of the whole process. Beside the conventional configuration, the model also includesdifferent alternative flow-patterns for the distillate and the vapor streams. An equation-oriented environmentwas selected to develop and implement the model, allowing different application instances, such assimulation, sensitivity analysis and optimization, to be easily performed.Simulation results have shown a good agreement with realistic design data and other authors' results. A rankingof themodel parameters is presented according to their impact on the heat transfer area.Moreover, optimizationresults showed that themodification of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.fied and detailed enough model to accurately predict the MEE system performance is presented. Themodel is highly nonlinear and it is based on mass and energy balances which are derived from a superstructure.The superstructure involves several process configurations which have to be simultaneously optimizedto determine the best stream flow-patterns (synthesis), the size of each evaporation effect (sizing) and theoperating conditions of the whole process. Beside the conventional configuration, the model also includesdifferent alternative flow-patterns for the distillate and the vapor streams. An equation-oriented environmentwas selected to develop and implement the model, allowing different application instances, such assimulation, sensitivity analysis and optimization, to be easily performed.Simulation results have shown a good agreement with realistic design data and other authors' results. A rankingof themodel parameters is presented according to their impact on the heat transfer area.Moreover, optimizationresults showed that themodification of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.figurations which have to be simultaneously optimizedto determine the best stream flow-patterns (synthesis), the size of each evaporation effect (sizing) and theoperating conditions of the whole process. Beside the conventional configuration, the model also includesdifferent alternative flow-patterns for the distillate and the vapor streams. An equation-oriented environmentwas selected to develop and implement the model, allowing different application instances, such assimulation, sensitivity analysis and optimization, to be easily performed.Simulation results have shown a good agreement with realistic design data and other authors' results. A rankingof themodel parameters is presented according to their impact on the heat transfer area.Moreover, optimizationresults showed that themodification of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.flow-patterns (synthesis), the size of each evaporation effect (sizing) and theoperating conditions of the whole process. Beside the conventional configuration, the model also includesdifferent alternative flow-patterns for the distillate and the vapor streams. An equation-oriented environmentwas selected to develop and implement the model, allowing different application instances, such assimulation, sensitivity analysis and optimization, to be easily performed.Simulation results have shown a good agreement with realistic design data and other authors' results. A rankingof themodel parameters is presented according to their impact on the heat transfer area.Moreover, optimizationresults showed that themodification of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.figuration, the model also includesdifferent alternative flow-patterns for the distillate and the vapor streams. An equation-oriented environmentwas selected to develop and implement the model, allowing different application instances, such assimulation, sensitivity analysis and optimization, to be easily performed.Simulation results have shown a good agreement with realistic design data and other authors' results. A rankingof themodel parameters is presented according to their impact on the heat transfer area.Moreover, optimizationresults showed that themodification of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.flow-patterns for the distillate and the vapor streams. An equation-oriented environmentwas selected to develop and implement the model, allowing different application instances, such assimulation, sensitivity analysis and optimization, to be easily performed.Simulation results have shown a good agreement with realistic design data and other authors' results. A rankingof themodel parameters is presented according to their impact on the heat transfer area.Moreover, optimizationresults showed that themodification of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.fication of the flowpatterns improve the process performance, reducing the processspecific total heat transfer area in about 5% compared to the optimal value of the conventional case.fic total heat transfer area in about 5% compared to the optimal value of the conventional case.","palabras_clave":"Multi-Effect Evaporation system|Optimization|Non-linear programming (NLP)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63166,"idioma_id":4,"titulo":"Impurity effects in two-electron coupled quantum dots: entanglement modulation","resumen":"                          \t    We present a detailed analysis of the electronic   and optical properties of two-electron quantum dots with a   two-dimensional Gaussian confinement potential. We study the effects of   Coulomb impurities and the possibility of manipulating the entanglement   of the electrons by controlling the confinement potential parameters.   The degree of entanglement becomes highly modulated by both the location   and charge screening of the impurity atom, resulting in two regimes:   one of low entanglement and the other of high entanglement, with both of   them mainly determined by the magnitude of the charge. It is shown that   the magnitude of the oscillator strength of the system could provide an   indication of the presence and characteristics of impurities and,   therefore, the degree of entanglement.                                                      ","palabras_clave":"Qauntum Dots|Entanglement|Optical Properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63167,"idioma_id":4,"titulo":"Phytoplankton summer bloom dynamics in the Bahía Blanca Estuary in relation to changing environmental conditions","resumen":"We examined austral summer phytoplankton data (December?March) that cover the years 1978?2008 and compared with physico-chemical and meteorological variations in the Bah&#305;´a Blanca Estuary,Argentina. During the years 1978?1982, 1992?1993 and 2006?2008, counts of phytoplankton abundance showed an increase in recent summers; from a mean value of 12103 cells L1 in 1978to 2239103 cells L1 in 2008, while the chlorophyll concentration remained relatively constant (8.572.5 mg L1, CV¼27%) over the continuous time Series (1978?2008). The rise in the ratio ?cell abundance: chlorophyll concentration? was linked to modifications in species composition, fromdominance of phytoflagellates (10?20 mm) and relatively large diatoms (e.g., Cyclotella striata 25?38 mm, Paralia sulcata 15?70 mm, Cerataulina pelagica 18?30 mm, Thalassiosra hendeyi 27?52 mm) towards the dominance of the small (5?15 mm) centric diatom Thalassiosira minima, which reached480% of the total phytoplankton abundance in summers 2006?2008. The Bah&#305;´a Blanca Estuary has undergone climate modifications and increasing anthropogenic disturbances during the last three decades. In the early 1990s, regional climatic conditions revealed a significant shift. Additionally, dredging activities were initiated to allow the traffic of large ships, rising the levels of suspended sediments, and the invasive copepod Eurytemora americana was introduced via ballast waters into theestuary and has displaced the dominance of the native copepod Acartia tonsa towards summer periods.The examination of physico-chemical conditions of the estuary showed a trend to increase in the minima of water temperature and higher water turbidity, dissolved phosphate, nitrite and nitrate concentrations in the pelagic environment in recent summers. We discuss the potential effects of these changes and trophic interactions on the structure and composition of the phytoplankton summer blooms in this temperate and eutrophic estuary in the Southwestern Atlantic.","palabras_clave":"phytoplankton summer blooms|physico-chemical modifications|dredging activities|resuspension|temperate estuary|trophic interactions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63168,"idioma_id":4,"titulo":"Melatonin, given at the time of reperfusion, prevents ventricular arrhythmias in isolated hearts from fructose-fed rats and spontaneously hypertensive rats","resumen":"Melatonin reduces reperfusion arrhythmias when administered before coronary occlusion, but in the clinical context of acute coronary syndromes, most of the therapies are administered at the time of reperfusion. Patients frequently have physiological modifications that can reduce the response to therapeutic interventions. This work determined whether acute melatonin administration starting at the moment of reperfusion protects against ventricular arrhythmias in Langendorff-perfused hearts isolated from fructose-fed rats (FFR), a dietary model of metabolic syndrome, and from spontaneous hypertensive rats (SHR). In both experimental models, we confirmed metabolic alterations, a reduction in myocardial total antioxidant capacity and an increase in arterial pressure and NADPH oxidase activity, and in FFR, we also found a decrease in eNOS activity. Melatonin (50 &#956;m) initiated at reperfusion after 15-min regional ischemia reduced the incidence of ventricular fibrillation from 83% to 33% for the WKY strain, from 92% to 25% in FFR, and from 100% to 33% in SHR (P = 0.0361, P = 0.0028, P = 0.0013, respectively, by Fisher´s exact test, n = 12 each). Although, ventricular tachycardia incidence was high at the beginning of reperfusion, the severity of the arrhythmias progressively declined in melatonin-treated hearts. Melatonin induced a shortening of the action potential duration at the beginning of reperfusion and in the SHR group also a faster recovery of action potential amplitude. We conclude that melatonin protects against ventricular fibrillation when administered at reperfusion, and these effects are maintained in hearts from rats exposed to major cardiovascular risk factors. These results further support the ongoing translation to clinical trials of this agent.","palabras_clave":"melatonin|spontaneously hypertensive rats|reperfusion|ischemic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63169,"idioma_id":4,"titulo":"Characterization of Shiga toxin-producing Escherichia coli O130:H11 and O178:H19 isolated from dairy cows","resumen":"Shiga toxin-producing E. coli (STEC) are isolated from human patients with bloody diarrhea, hemorrhagic colitis (HC), and hemolytic uremic syndrome (HUS). In the last years, the infections with non-O157 serotypes are increasing their frequency of association with human disease. STEC produce Shiga toxin (Stx) and other virulence factors that could contribute to human pathogenesis. Cattle are the main reservoir and the transmission to humans is through the consumption of undercooked meat, non-pasteurized dairy products, and vegetables or water contaminated with feces. We have previously determined that O130:H11 and O178:H19 serotypes were the most prevalent in dairy cows from Argentina. In the present study, 37 and 25 STEC isolates from dairy cows belonging to O130:H11 and O178:H19 serotypes, respectively, were characterized regarding to their cytotoxicity on Vero cells, stx subtypes, presence of sab and typing by multiple-locus variable-number tandem repeat analysis (MLVA). All strains demonstrated a cytotoxic effect, and in O130:H11 isolates, stx2EDL933 was the predominant subtype. In O178:H19 isolates the main stx2 subtype was stx2vha. The sab gene was detected in 65 and 24% of the isolates belonging to O130:H11 and O178:H19, respectively. Only one MLVA profile was identified among the O130:H11 isolates meanwhile 10 MLVA profiles were detected among the O178:H19 isolates which were grouped in two main clusters. In conclusion, our data show that O130:H11 and O178:H19 STEC isolates encode virulence factors associated with severe human disease and both serotypes should be considered for routinely testing. Our subtyping experiments showed that isolates could be distinguished based on the stx2 subtype and the presence\/absence of sab gene, and for isolates belonging to O178:H19, also when the MLVA type was considered. However, MLVA subtyping of O130:H11 isolates will require the development of more specific markers. ","palabras_clave":"STEC|dairycattle|MLVA|Shigatoxin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63170,"idioma_id":4,"titulo":"Receptor-mediated endocytosis and trafficking between endosomal-lysosomal vacuoles in Giardia lamblia.","resumen":"The early branching Giardia lambliahas highly polarized vacuoles, located underneath the plasma mem-brane, which have at least some of the characteristics ofendosomes and of lysosomes. These peripheral vacuoles  (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic  in trophozoites with a rate similar to the internalization ofthe low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We  speculate on PV functional heterogeneity and maturation in this parasite.","palabras_clave":"Giardia lamblia|Receptor-mediated endocytosis|Peripheral vacuoles|Low-density lipoprotein"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63171,"idioma_id":4,"titulo":"Temperature affects the inhalant siphon regeneration of Amiantis purpurata (Lamarck, 1818) (Bivalvia; Veneridae)","resumen":"The purple clam Amiantis purpurata, inhabits intertidal and shallow subtidal  platforms from San Matías Gulf, Argentina to Santa Catarina, Brazil. This edible  bivalve is an important component of the marine trophic webs due to siphon nipping.  The present study investigates the influence of temperature on the regeneration of the  inhalant siphon tentacle crown of A. purpurata. Under laboratory conditions 140 purple  clams were exposed to four different temperatures (12, 15, 20 and 22 ºC). At each  temperature, the distal 0.5 cm of the inhalant siphon was removed from each clam. The  length of long tentacles from each group was measured on different days until the  tentacles had reached asymptotic growth. Four candidate models were used for each  tentacle dataset for growth at the four experimental temperatures. The data was fitted to  size-increments for the long siphon at different temperatures using maximum  likelihood. The von Bertalanffy model was the one that best fitted the data at most  experimental temperatures. To evaluate differences in tentacle regeneration in each  treatment, the growth parameters were compared using a likelihood ratio test. The two  interdependent parameters affecting the regeneration process k (growth constant) and  ti.p. (inflexion point), were clearly influenced by temperature. In the case of k, increasing  temperature produces acceleration in the regeneration process while ti.p is not dependent  on temperature. Regeneration was slower than in any other species previously reported  in the literature and decreasing temperature slowed down the process.","palabras_clave":"PURPLE CLAM|SIPHON CROPPING|SOUTH-WESTERN ATLANTIC|INHALANT SIPHON"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63172,"idioma_id":4,"titulo":"A histopathological survey of the razor clam Ensis macha (Pharidae) along the Patagonian Argentina coast","resumen":"This is the first study performed to determine the health status of the razor clam, Ensis macha, including six different populations along Argentina Patagonian coast and one of Chile. The parasites and pathologies affecting E. macha were analyzed and their prevalence and mean intensity values were calculated.To establish which factors affect the presence and intensity of infection, Generalized Linear Models  (GLMs) were applied. Basophilic inclusions, ciliates, coccidians protozoans and turbellarians were found. We report an Aporocotylidae digenean and hemocyte infiltrations. None of the parasites is OIE (World  Organisation for Animal Health) notifiable, and none seemed to be pathogenic, with the exception of the digenean. The prevalence of the parasites was affected mainly by environmental factors (such as site  of sampling and season) instead of intrinsic conditions of the clam (such as size, condition index, sex and gonadal stage). On the other hand, the maximum intensity of parasites was not only related with cold  seasons but also with the partially spawned gonadal stage of E. macha. During this stage, the clams would need to store energy for the next gametogenesis cycle, might be more susceptible to infection by the  parasites.","palabras_clave":"ENSIS MACHA|HEALTH STATUS|PATAGONIAN COAST|PARASITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63173,"idioma_id":4,"titulo":"Some factors affecting the number of days open in Argentinean dairy herds","resumen":"The purpose of this study was to estimate, the relative contribution of factors affecting how quickly cattle become pregnant in Argentinean dairy herds. A total of 76,401 cows corresponding to 249 dairy herds were analyzed. A hazard model was adjusted to explore days open (DO). The factors considered were milk yield, lactation number, calving season and breeding technique [i.e. type of service: artificial insemination (AI), and combined service].  Cows of lower milk yield had a 1.09 to 1.38 higher likelihood to get pregnant than those animals of higher production (P<0.0001).The number of DO increased linearly with the increasing number of lactations (P<0.0001). Cows calving in the fall-winter had a shorter time to conception than cows calving in the summer. The HR for combined service was 1.27, indicating that cows with combined service are more likely to becoming pregnant over the observation period than those bred by AI. The difference between high and low milk yield cows regard to the proportion of pregnant cows was higher under combined service than AI. Furthermore, dairies using mainly combined service had lower milk production (5,693.7 L) than those using mainly AI (7,684.4 L). While lactation number and calving season are contributing factors to explain the number of days open, the influence of production level, the type of service and the interaction between the two also appear as determinant factors influencing reproductive efficiency in Argentinean dairy herds.","palabras_clave":"MILK YIELD|OPEN COWS|HAZARD MODEL|ARTIFICIAL INSEMINATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63174,"idioma_id":4,"titulo":"Surgical Lung Biopsy in Transplant Patients with Diffuse Lung Disease: How Much Worse When the Lung Is the Graft?","resumen":"BACKGROUND: There are no data that compare the clinical presentation and results of surgical lung biopsy (SLB) for diffuse lung disease (DLD) in lung transplant patients, in contrast to individuals with other type of solid organ grafts. Our objective was to compare the clinical picture, radiologic pattern, pathology results, and outcomes of SLB for DLD in these two subsets of patients. METHODS: We retrospectively reviewed the clinical records of transplant patients undergoing SLB for DLD at our institution between 2004 and 2011. Patients with lung transplants and those with other transplants were compared. RESULTS: During the study period, 1,232 solid organ transplants were done at our institution. Of these, 49 patients (4%) had DLD that needed SLB for diagnosis, and 24 of these patients had a lung transplant. Dyspnea and a radiologic reticular pattern were more frequent in lung transplant patients, 21 of 24 vs 11 of 25 (p = 0.001) and 14 of 24 vs 7 of 25 (p = 0.03), respectively. Although postoperative complications and in-hospital deaths were more common in lung transplant patients, the differences were not statistically significant. Having the SLB performed for diagnosis, as opposed to being conducted for DLD that did not improve on medical treatment, had a protective effect on multivariate analysis (hazard ratio, 0.39; 95% confidence interval, 0.16 to 0.96; p = 0.042). A prior lung transplant was the only independent predictor of survival (hazard ratio, 4.62; 95% confidence interval, 1.53 to 13.92, p = 0.006). CONCLUSIONS: It is relatively uncommon for a solid organ transplant patient with DLD to require a SLB. Clinical and radiologic presentation differ in patients with lung transplants compared with other transplants. Postoperative outcomes are not significantly different between the groups. SLB performed early in the course of the disease might be beneficial. Having a lung transplant is a significant negative predictor of survival.","palabras_clave":"lung surgery|pathology|biopsy|transplantation|prognosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63175,"idioma_id":4,"titulo":"Osmotic induction marking with Alizarin Red S on pejerrey, Odontesthes bonariensis juveniles","resumen":"Juveniles of pejerrey, Odontesthes bonariensis, were exposed to 0.1% Alizarin Red S (ARS) alone or with a previous immersion in 2.2% saline solution (Osmotic Induction, OI) to enhance the ARS marking method. The procedures were performed in the field, and fish were then released in 1 m3 cages in ?La Salada de Monasterio? lagoon, Chascomús, Buenos Aires, Argentina. After 73 days, clear marks were observed in the otoliths, caudal fin rays and scales with both treatments, being the intensity of the signal in the scales of OI+ARS treated fish higher. On the other hand, no marks were observed in the control group on the same structures. Approximately one year post-treatment (385 days), only marks in caudal fin rays were found clearly in OI+ARS treated fish. After this period, no significant differences in total length or weight between marked or control fish were observed and the mortality ranged between 30-40 % in all cages. These results provide strong evidence for the potential applicability of this cost-effective marking technique in differentiation of wild and hatchery-produced pejerrey, a much needed step in the advance of the O. bonariensis propagation and extensive aquaculture. The success in the caudal fin rays marking is also important because it is easy to do and does not require the sacrifice of fish.","palabras_clave":"ALIZARIN|FIN RAYS|OTOLITHS|PEJERREY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63176,"idioma_id":4,"titulo":"Genotypic sex determination in teleosts: insights from the testis-determining amhy gene","resumen":"The master sex-determining genes identified so far in fishes are clearly not conserved, as evidenced by several unrelated genes reported to play critical roles in sex determination. In this study, we reviewed the molecular process of sex determination in the Patagonian pejerrey Odontesthes hatcheri, an emerging model due to the recent discovery that a Y-chromosome linked, duplicated copy of the anti-Müllerian hormone gene, amhy plays a pivotal role in sex determination. A comparative analysis with other newly found sex-determining genes of teleost fish, DMY\/dmrt1bY, sdY, amhr2, andgsdfY is performed and alternative ideas are proposed to explain the mechanism involved in the rise of various types of non-homologous sex-determining genes.","palabras_clave":"Odontesthes hatcheri|Patagonian pejerrey|GSD|amhy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63177,"idioma_id":4,"titulo":"Environmental stress-induced testis differentiation: androgen as a by-product of cortisol inactivation","resumen":"This review deals with the gonadal  masculinization induced by thermal stress in fish with focus on the action of  11&#946;-hydroxysteroid dehydrogenase (11&#946;-HSD) as this mechanism key transducer. High temperatures have  been reported to produce male-skewed sex ratios in several species with TSD (Temperature-dependent  Sex Determination), and in some of them, this process was reported to be  associated with high levels of cortisol, the hormone-related stress in  vertebrates, during early gonad development. In addition, in pejerrey larvae  reared at high-masculinizing temperatures, 11-ketotestosterone (11-KT), the  main and most potent androgen in fish, was also detected at high levels. In testicular explants, cortisol induced the synthesis of 11-KT, suggesting that its synthesis could  be under the control of the stress axis at the time of gonadal fate  determination. 11&#946;-HSD is one of the enzymes shared by the  glucocorticoid and androgen pathways; this enzyme converts cortisol to  cortisone and also participates in the finals steps of the synthesis of the 11-oxigenated  androgens. Based on these data and literature information, here we propose that the  masculinization induced by thermal stress can be considered as a consequence of  cortisol inactivation and the concomitant synthesis of 11-KT and discussing  this as a possible mechanism of masculinization induced by different types of  environmental stressors.","palabras_clave":"Environmental sex determination|stress|masculinization|11&#946;-HSD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63178,"idioma_id":5,"titulo":"Selection of Induction Machine Models for Efficiency Evaluation in Electric Vehicles","resumen":"Abstract\u2014 Different induction machine (IM) models are evaluated, with the aim to determine its usefulness in efficiency studies for electric vehicles (EV) traction drives. In particular, two simple IM models proposed in the literature, which take into account the additional losses, are compared. With this aim, model parameters are adjusted from experimental tests performed on a 4 kW IM. Then, results obtained from both models at different operating points are compared. Even when the estimation errors are similar when using both models, one of them is chosen because of its simplicity. Finally, a simple application of this model is proposed, which consists in use the model for obtaining a complete efficiency map of the IM, without need further essays. Such efficiency map can be used to evaluate the overall efficiency of the traction drive of an EV, or in EV design.","palabras_clave":"INDUCTION MACHINE|MODELS|LOSSES|ELECTRIC VEHICLES|EFFICIENCY MAPS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63179,"idioma_id":4,"titulo":"Role of He excited configurations in the neutralization of He+ ions colliding with a HOPG surface","resumen":"The very high neutral fractions measured in He+ scattered by graphitelike surfaces, at intermediate incoming energies (1 keV &lt; Ein &lt; 6 keV), cannot be explained only by the resonance of the He ionization level with the valence band states of the surface. Excited configurations (1s2s) and (1s2p) appear as possible resonant neutralization channels together with the ground state one (1s2). We develop, in this work, a time-dependent quantum-mechanical calculation of the charge-transfer process in He+\/HOPG collision, where the resonant neutralization to the ground and first excited states of He is taken into account.We use an Anderson Hamiltonian projected on the electronic configurations of the projectile atom which are energetically favorable for the charge-exchange process. Thus, an exhaustive analysis of different possible approximations to the&nbsp; neutralization of He+ is performed: the typical neutralization to the ground state by either neglecting or not the electron spin and finally the one including excited configurations.Our results reproduce&nbsp; the observed experimental trends only when excited configurations (1s2s) and (1s2p) are involved in the charge exchange between the ion and the surface.","palabras_clave":"He+\/HOPG COLLISION| CHARGE EXCHANGE PROCESS|EXCITED CONFIGURATIONS |CORRELATION EFFECTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63180,"idioma_id":4,"titulo":"Fungal root colonization of Puccinellia frigida (Phil.) Johnston, a dominant grass species inhabiting the margins of high-altitude hypersaline Andean wetlands","resumen":"High-altitude hypersaline Andean wetlands are considered stressful environments by the prevalence of extreme abiotic conditions affecting both plant host and fungal root endophytes. Puccinellia frigida (Phil.) Johnston, a dominant plant species inhabiting the margins of these wetlands over 4000 m.a.s.l. in Northwest Argentina, is frequently colonized by fungal root endophytes. Here we examined the nature and dynamics of fungal root colonization on this plant species in three different wetlands (six sampling sites) and two growing seasons. Morphologically diverse septate fungi and chytrids were observed in root samples of P. frigida in all sampling sites, whereas arbuscular mycorrhizal fungi were found only in two sites. The level of colonization of chytrids and septate fungi differed significantly both between sites and seasons. Soil organic matter and electrical conductivity were the only habitat parameters significantly related to the observed differences. The widespread occurrence of non-destructive fungal associations suggest an important role for plant survival in extreme environments.","palabras_clave":"MYCORRHIZA |STRESSFUL ENVIRONMENT |SALINITY |ANDEAN WETLANDS|PUCCINELLIA FRIGIDA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63181,"idioma_id":4,"titulo":"Characterization of egg yolk immunoglobulin (IgY) against enterotoxigenic Escherichia coli and evaluation of its effects on bovine intestinal cells","resumen":"            Enterotoxigenic Escherichia coli (ETEC) infection is common in calves. Egg yolk antibodies (IgY) have been used to treat gastrointestinal infectious diseases. This study aimed to characterize IgY against bovine ETEC and to evaluate its effects on bovine intestinal cell culture challenged with a bovine ETEC strain. IgY was isolated from the egg yolks of hens immunized with ETEC. The characteristics of IgY were determined by Bradford, ELISA, gel electrophoresis and immunoblotting. Significant differences in anti-ETEC activity between anti-ETEC IgY and non-specific IgY were found in lyophilized fractions. In the bacterial growth assay, anti-ETEC IgY (40 mg\/mL) showed growth inhibition of ETEC after 2 h of incubation (p&lt;0.05). The difference in bacterial growth between anti-ETEC IgY and non-specific IgY groups was 0.51 log CFU\/ml after an 8 h incubation (p&lt;0.05). The bacterial adhesion assay indicated that anti-ETEC IgY (40 mg\/ml) significantly decreased the adhesion of ETEC to bovine intestinal epithelial cells within 4 h (about 1.36 log units compared with the control group; p&lt;0.05). This study demonstrates that anti-ETEC IgY inhibits the growth and adherence of ETEC to bovine intestinal cells and is a potential alternative to traditional treatments of E. coli infections. ","palabras_clave":"Immunoglobulin Y (IgY)|Neonatal calf diarrhea|Enterotoxigenic Escherichia coli|Bacterial adherence"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63182,"idioma_id":4,"titulo":"Evaluation of an ensemble of regional climate model simulations over South America driven by the ERA-Interim reanalysis: model performance and uncertainties","resumen":"The capability of a set of 7 coordinated regional climate model simulations performed in theframework of the CLARIS-LPB Project in reproducing the mean climate conditions over the South American continent has been evaluated. The model simulations were forced by the ERA-Interim reanalysis dataset for the period 1990?2008 on a grid resolution of 50 km, following the CORDEX protocol. The analysis was focused on evaluating the reliability of simulating mean precipitation and surface air temperature, which are the variables most commonly used for impact studies. Both the common features and the differences among individual models have been evaluated and compared against several observational datasets. In this study the ensemble bias and the degree of agreement among individual models havebeen quantified. The evaluation was focused on the seasonal means, the area-averaged annual cycles and the frequency distributions of monthly means over target subregions.Results show that the Regional Climate Model ensemble reproduces adequately well these features, with biases mostly within ±2 C and ±20 % for temperature and precipitation, respectively. However, the multi-model ensemble depicts larger biases and larger uncertainty (as defined by the standard deviation of the models) over tropical regions compared with subtropical regions. Though some systematic biases were detected particularly over the La Plata Basin region, such as underestimation of rainfall during winter months and overestimation of temperature during summer months, every model shares asimilar behavior and, consequently, the uncertainty in simulating current climate conditions is low. Every model is able to capture the variety in the shape of the frequency distribution for both temperature and precipitation along the South American continent. Differences among individual models and observations revealed the nature of individual model biases, showing either a shift in the distribution or an overestimation or underestimation of therange of variability.","palabras_clave":"regional climate|model performance|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63183,"idioma_id":4,"titulo":"Influence of silica and coupling agent loading on thermal, morphological and mechanical properties of hybrid membranes","resumen":"Hybrid polyetherimide-silica (PEI-SiO2) membranes were synthesized via the sol?gel method through the hydrolysis of tetraethoxysilane (TEOS) with 3-aminopropyltriethoxysilane (APTEOS) as a coupling agent. The effects of silica content and the APTEOS\/TEOS ratio in thermal, morphological and mechanical properties of the hybrid membranes were studied. Although many studies report improvements in the structure or properties of composite materials when a coupling agent is used, these contributions do not investigate the effect of the coupling agent loading on the properties of the new material synthesized. In this study, we prepared hybrid membrane with a fixed amount of APTEOS modifying TEOS content to analyze the silica content effect. Conversely, to determine the coupling agent effect, hybrid membranes were prepared varying the APTEOS content while the amount of TEOS was kept constant. All hybrid membranes have a dense and uniform internal structure and exhibit good thermal resistance with a degradation temperature above 544 C. Membranes with 10 wt% of silica showed better tensile strength with a high modulus and low rupture elongation indicating an effective load transfer between the phases. Concerning the coupling agent effect, the maximum modulus was observed for membranes with 10 wt% of APTEOS, suggesting a greater interaction between the organic and inorganic phases.","palabras_clave":"POLYETHERIMIDE _ SILICA|HYBRID MEMBRANES |THERMAL PROPERTIES |MECHANICAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63184,"idioma_id":4,"titulo":"'Tripanocide and antibacterial activity of Alvaradoa subovata Cronquist extracts'","resumen":"We studied antioxidant, antibacterial and tripanocide activities of Alvaradoa subovata extracts. The ethanolic extracts showed the greatest DPPH radical scavenging capacity, especially that of bark with an IC50 = 4.7 ± 0.18 &#956;g\/mL. Wood dichloromethane extract displayed growth inhibition of the phytopathogenic bacteria Xanthomonas axonopodis in the disk diffusion assay and showed a MIC value of 100 &#956;g\/m.. It also showed growth inhibition of Trypanosoma cruzi (IC50 = 0.063 ± 0.003 mg\/mL). A fraction of this extract, which has emodin as the main component, showed tripanocide activity (60% of growth inhibition at 100 ug\/mL). The main compounds in wood dichloromethane extract were anthraquinones identified as chrysophanol and emodin, and scopoletin was identified. These three compounds could serve as analytical markers of the extract. The results of this study show that wood extract of A. subovata constitute a source of bioactive compounds such as antiparasitic and pesticides agents.","palabras_clave":"Alvaradoa subovata|DPPH|Picramniaceae|Xanthomonas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63185,"idioma_id":4,"titulo":"An odd case of heteroallelic acute intermittent porphyria in the argentinean population","resumen":"AbstractAIP is an acute liver disorder caused by a deficiency of porphobilinogen deaminase (PBGD) characterized by neuroabdominalsymptoms. It is an autosomal dominant disease. However, homozygous dominant AIP (HD-AIP) have been described.In some cases erythrodontia was observed. CEP is an autosomal recessive disease produced by mutations in the uroporphyrinogenIII synthase gene (UROS), characterized by severe cutaneous lesions and erythrodontia. The aim of the work was toestablish the differential diagnosis of porphyria in a patient with abdominal pain, neurological attacks, skin symptoms anderythrodontia. The PBGD activity was reduced 50% and the genetic analysis indicated the presence of two genetic variantsin the PBGD gene, p.G111R and p.E258G, a new genetic variant, revealing a case of heteroallelic HD-AIP. The patient, firstdiagnosed as a carrier of a dual porphyria: AIP \/ CEP based on the excretion profile of porphyrins, precursors and her clinicalsymptoms, would be an atypical case of human HD-AIP. These results would also suggest the presence of a phenocopy ofthe CEP, induced by an endogenous or exogenous factor. Our findings highlight the importance of genetic studies for a properdiagnosis of porphyria, prevention of its manifestation and its treatment.","palabras_clave":"Porfiria Aguda Intermitente|Porfobilinogeno deaminasa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63186,"idioma_id":4,"titulo":"Attaichnus kuenzelii revisited: a Miocene record of fungus-growing ants from Argentina","resumen":"Attaichnus kuenzelii is a nest of leaf-cutting ants from the Miocene of La Pampa, Argentina, described by Laza (1982) in a pioneer paper for insect palaeoichnology. The type locality was revisited to update morphological description, affinities, and palaeoenvironmental conditions. In present day outcrops, A. kuenzelii shows nomounds or high concentration of interconnected chambers as originally described, but well separated and regularly distributed chambers, which at most may compose groups of two or three. This updated morphologysuggests that A. kuenzelii can be attributed to Attini other than Atta, probably species of Acromyrmex or Trachymyrmex. Associated trace fossils are large vertebrate burrows, rhizoliths, meniscate burrows, and scarceCoprinisphaera. Other specimens of dung beetle and bee trace fossils found in the same palaeosolswere probably constructed by younger producers on soils developed on post-Miocene sediments, which are mostly eroded today. Evidence for this assumption is the lack of carbonate cementation and presence of organic remains in these traces. The reinterpretation of A. kuenzelii as a nest of species of Acromyrmex or Trachymyrmex modifies the original assumption of a northern subtropical palaeoenvironment during the Miocene for this area based on the distribution of Atta species. The new affinities proposed in combination with the studied palaeosols interpreted as mollisols, phytoliths, and previous palaeontological, and sedimentological evidence suggest anopen palaeoenvironment under a temperate climate, dominated by grasses.","palabras_clave":"ATTAICHNUS|ANT NEST|PALAEOENVIRONMENT|MIOCENE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63187,"idioma_id":4,"titulo":"The South American ancestry of the North Patagonian Massif: geochronological evidence for an autochthonous origin?","resumen":"New U-Pb and 40Ar\/39Ar age data from deformed and undeformed granitoids of the North Patagonian Massif establish  the presence of Early Cambrian and widespread Ordovician  magmatism in northern Patagonia. These data suggest that  the Pampean (Cambrian) and Famatinian (Ordovician) magmatic belts of the Sierras Pampeanas are continuous into  Patagonia. SHRIMP U-Pb age spectra from detrital zircons of  Cambro-Ordovician metasedimentary rocks show patterns  very similar to those from equivalent units of the Pampia  block, over 500 km farther north. These results suggest that  the North Patagonian Massif was likely part of the South  American margin of Gondwana in the early Palaeozoic and  strongly argue in favour of an authochtonous or para-autochthonous origin for this block.","palabras_clave":"Patagonia|Magmatism|Terranes|Gondwana"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63188,"idioma_id":4,"titulo":"Mycoflora and Potential Mycotoxin Production of Freshly Harvested Blueberry in Concordia, Entre Ríos Province, Argentina","resumen":"Blueberries cv. Misty (2009) and cvs. Emerald, Jewel, andMisty (2010) were analyzed for mycoflora occurrence in themain production area of Argentina. Isolation frequencies andrelative densities of the fungal species were statistically compared.Alternaria tenuissima was predominant and the potentialpresence of Alternaria toxins may pose a risk for blueberriesconsumption. This is the first report of Aspergillusflavus, Alternaria alternata, Alternaria vaccinii, Arthriniumphaeospermum, Cladosporium cladosporioides, Curvularia lunata,Epicoccum nigrum, Eurotium chevalieri, Fusarium graminearum,Fusarium semitectum, Fusarium verticillioides, Geotrichum candidum,Mucor racemosus, Penicillium citrinum, Trichodermaharzianum and Trichocladium spp. isolated from blueberries inArgentina.","palabras_clave":"BLUEBERRY CULTIVARS|FUNGI|ALTERNARIA|MYCOTOXINS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63189,"idioma_id":4,"titulo":"Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina","resumen":"A total of 89 freshly harvested soybean seed samples (Roundup Ready [transgenic] soybean cultivars) from the 2010\/2011 crop season were collected from five locations in the Northern Pampean Region II, Argentina. These samples were analyzed for internal mycoflora, toxin production of isolated fungi, and for a range of mycotoxins. Mycotoxin analysis of aflatoxins (AFs), zearalenone (ZEA), fumonisins (FBs) and ochratoxin A (OTA) was done by HPLC-FLD (high performance liquid chromatography with postcolumn fluorescence derivatization), alternariol and alternariol monomethyl ether with HPLC-UV (HPLC with UV detection), trichothecenes (deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin, fusarenon X, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol were analyzed by GC-ECD (gas chromatography with electron capture detector). Fungal colonization was more frequently found for samples from America, Saladillo and Trenque Lauquen than for samples from General Villegas and Trenel a total of 1,401 fungal isolates were obtained from the soybean seeds. The most commonly identified fungal genera were Alternaria, Sclerotinia, Chaetomium, Cladosporium, Aspergillus, Penicillium, Phomopsis and Fusarium. Alternaria alternata, A.tenuissima, Aspergillus flavus, Penicillium citrinum, Fusarium verticillioides and F.semitectum were the predominant toxigenic fungal species. Mycotoxin production was confirmed for several isolates of toxigenic species, including Aspergillus flavus, A. parasiticus, Alternaria alternata, A.tenuissima, Fusarium graminearum, F semitectum and F. verticillioides. In particular, the percentage of mycotoxigenic Alternaria alternata (100 %), A.tenuissima (95 %) and aflatoxigenic strains of A. flavus (57 %) were remarkably high. Although none of the mycotoxins, AFs, ZEA, FBs, trichothecenes and OTA, were directly detected in samples of soybean seeds, the frequent presence of toxigenic fungal species indicates the risk of multiple mycotoxin contamination.","palabras_clave":"SOYBEAN|FUNGI|MYCOTOXINS|ALTERNARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63190,"idioma_id":4,"titulo":"Mycobiota and Potential Mycotoxin Contamination of Soybean RR in Different Production Areas in Argentina","resumen":"A total of 348 freshly harvested soybean samples from a multi-environment trial, conducted inexperimental fields belonging to INTA (Instituto Nacional de Tecnología Agropecuaria) in 12 localities, were analyzed for mycotoxin natural occurrence and for determining the associated endogenous mycoflora. Aflatoxins (AFs) and zearalenone (ZEN) were analyzed by HPLC and deoxynivalenol (DON) by CG.Samples from Reconquista, Tres Pozos, Rafaela and Manfredi were the most infested by fungi and those from Barrow and Balcarce the less contaminated. A total of 13,316 fungal isolates were identified from the seeds of 32 Roundup® Ready (transgenic) soybean cultivars. All fungi isolated were mitosporic fungi and ascomycetes. The most common fungi identified included species that belong to Alternaria, Fusarium, Sclerotinia, Phomopsis, Rhizoctonia and Cladosporium genera. The isolation frequencies and relative densities of species were calculated. Alternaria alternata, Fusarium equiseti, Sclerotinia sclerotiorum, Phomopsis spp., Fusarium semitectum, Cladosporium cladosporioides and Rhizoctonia solani were the predominant fungal species identified as endogenous mycoflora. No soybean samples were naturally contaminated with AFs, DON or ZEN. This is the first report on contaminant mycoflora and mycotoxin natural occurrence in transgenic soybean seeds from an extensive production area in Argentina.","palabras_clave":"SOYBEAN|MYCOFLORA|TRANSGENIC|ALTERNARIA|FUSARIUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63191,"idioma_id":4,"titulo":"Phylogenetic relationships of a patagonian frog radiation, the Alsodes + Eupsophus clade (Anura, Alsodidae) with comments on the supposed paraphyly of Eupsophus","resumen":"The frog clade composed of the alsodid genera Alsodes + Eupsophus is the most species-rich of the Patagonian endemic frog clades, including nearly 31 of the slightly more than 50 species of that region. The biology of this group of frogs is poorly known, its taxonomy quite complex (particularly Alsodes), and its diversity in chromosome number striking when compared with other frogs (collectively, there are species having 2n = 22, 2n = 26, 2n = 28, 2n = 30 or 2n = 34). We present&nbsp; a phylogenetic analysis of this Patagonian frog clade based on mitochondrial and nuclear gene sequences. We sequenced five mitochondrial genes (cytochrome b, cytochrome oxidase I, 12S, 16S, NADH dehydrogenase subunit 1) with three intervening tRNAs, and fragments of three&nbsp; nuclear genes (seven in absentia homolog 1, rhodopsin exon 1, RAG-1), for a maximum of 6510 bp for multiple specimens from 26 of the 31 species. We recovered Eupsophus as polyphyletic, with E. antartandicus, E. sylvaticus, and E. taeniatus in Batrachylidae, in accordance with most previous hypotheses. Based on this result, we transfer E. antartandicus and E. taeniatus back to Batrachyla, and E. sylvaticus to Hylorina (resurrected from the synonymy of Eupsophus), remediating the paraphyly of Eupsophus. Our results strongly corroborate the monophyly of Alsodes + Eupsophus (sensu stricto), the individual monophyly of these genera, and the monophyly of the species groups of Eupsophus. They also show the non-monophyly of all non-monotypic species groups of Alsodes proposed in the past. Our results expose several taxonomic problems particularly in Alsodes, and to a lesser extent in Eupsophus. This phylogenetic context suggests a rich evolutionary history of karyotypic diversification in the clade, in part corroborating previous hypotheses. In Alsodes, we predict three independent transformations of chromosome number from the plesiomorphic 2n = 26. All these, strikingly, involve increments or reductions of pairs of&nbsp; haploid chromosomes. Finally, the phylogenetic pattern recovered for Alsodes and Eupsophus suggests a trans-Andean origin and diversification of the group, with multiple, independent ingressions over cis-Andean regions.","palabras_clave":"Alsodes|Eupsophus|Filogenia|Batrachyla"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63192,"idioma_id":4,"titulo":"Millimeter length micromachining using a heavy ion nuclear microprobe with standard magnetic scanning","resumen":"Abstract: In order to increase the scanning length of our microprobe, we have developed an irradiation procedure suitable for use in any nuclear microprobe, extending at least up to 400% the length of our heavy ion direct writing facility using standard magnetic exploration. Although this method is limited to patterns of a few millimeters in only one direction, it is useful for the manufacture of curved waveguides, optical devices such Mach\u2013Zehnder modulators, directional couplers as well as channels for micro-fluidic applications.As an example, this technique was applied to the fabrication of 3 mm 3D-Mach\u2013Zehnder modulators in lithium niobate with short Y input\/output branches and long shaped parallel-capacitor control electrodes. To extend and improve the quality of the machined structures we developed new scanning control software in LabView™ platform. The new code supports an external dose normalization,electrostatic beam blanking and is capable of scanning figures at 16 bit resolution using a National Instruments ™ PCI-6731 High-Speed I\/O card. A deep and vertical micromachining process using swift 35Cl ions 70 MeV bombarding energy and direct write patterning was performed on LiNbO3, a material which exhibits a strong natural anisotropy to conventional etching. The micromachined structures show the feasibility of this method for manufacturing micro-fluidic channels as well.","palabras_clave":"Direct write|Swift heavy ions|Lithium Niobate|Wave guides"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63193,"idioma_id":4,"titulo":"LATIN AMERICAN DEMOCRACY. WHAT TO DO WITH THE LEADERS?","resumen":"The democratic deficit or the gap between citizens\u2019 aspirations and their level of satisfaction is increasing in Latin America. Such dissatisfaction helps to understand many of the region\u2019s presidential crises: since 1985, 23 Latin American presidents have left government abruptly. While civil society may have been able to provoke the fall of presidents, it has not managed to avoid the re-emergence of deep-rooted political practices under subsequent administrations. Extreme presidentialism, clientelism and populism have re-emerged strengthened after deep political crises. This article offers some ideas regarding the impact that different types of political leaders can have on how well democracy works.","palabras_clave":"LEADERS|POLITICAL PARTIES|INSTITUTIONALISATION|ARGENTINA, ECUADOR, URUGUAY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63194,"idioma_id":4,"titulo":"Anandamide inhibits transport-related oxygen consumption in the loop of Henle by activating CB1 receptors","resumen":"The energy required for active Na chloride reabsorption in the thick ascending limb (TAL) depends on oxygen consumption and oxidative phosphorylation (OXP). In other cells, Na transport is inhibited by the endogenous cannabinoid anandamide through the activation of the cannabinoid receptors (CB) type 1 and 2. However, it is unclear whether anandamide alters TAL transport and the mechanisms that could be involved. We hypothesized that anandamide inhibits TAL transport via activation of CB1 receptors and NO. For this, we measured oxygen consumption (QO2) in TAL suspensions to monitor the anandamide effects on transport and OXP. Anandamide reduced QO2 in a concentration-dependent manner. During Na-K-2Cl cotransport and Na\/H exchange inhibition, anandamide did not inhibit TAL QO2. To test the role of the cannabinoid receptors, we used specific agonists and antagonists of CB1 and CB2 receptors. The CB1-selective agonist WIN55212\u20132 reduced QO2 in a concentration-dependent manner. Also, the CB1 receptor antagonist rimonabant blocked the effect of anandamide on QO2. In contrast, the CB2-selective agonist JHW-133 had no effect on QO2, while the CB2 receptor antagonist AM-630 failed to block the anandamide effects on QO2. To confirm these results, we measured CB1 and CB2 receptor expression and only CB1 expression was detected. Because CB1 receptors are strong nitric oxide synthase (NOS) stimulators and NO inhibits transport in TALs, we evaluated the role of NO. Anandamide stimulated NO production and the NOS inhibitor NG-nitro-L-arginine methyl ester blocked the anandamide effects on QO2. We conclude that anandamide inhibits TAL Na transport-related QO2 via activation of CB1 receptor and NOS.","palabras_clave":"Hipertension|Reabsorcion de sal|NHE|NKCC2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63196,"idioma_id":5,"titulo":"Aislamiento de Leptospira borgpetersenii de fuentes de agua en argentina","resumen":"las especies del género Leptospira son los agentes causales de la leptospirosis, enfermedad considerada como la zoonosis de mayor distribución en el mundo. En Argentina reviste carácter endémico. La provincia de Santa Fe registra el mayor número de casos humanos. Desde mediados de la década de 1980, las especies patógenas de leptospiras aisladas de animales y humanos fueron diferenciadas sobre la base de estudios de hibridización ADN-ADN, surgiendo nuevas especies: L. interrogans, L. kirschneri, L. weilii, L. noguchii, L. borgpetersenii, L. santarosai, L. meyeri, L. inadai, L. faineri y L. alexanderi. Objetivo: aislar y caracterizar mediante métodos moleculares, leptospiras de fuentes de agua que vierten en un canal que atraviesa la ciudad de Casilda, Santa Fe, en Argentina. Métodos: se sembraron 6 muestras de agua de las vertientes al canal, previa filtración con filtros Millipore de 0,22 µm, en medios EMJH y Fletcher para aislamiento de leptospiras. Se incubaron en estufa a 30 °C durante 15 días, se observaron semanalmente mediante microscopia de campo oscuro. Se implementó la técnica de reacción en cadena de la polimerasa bajo las condiciones específicas (Sugathan, 2005), con dos juegos de cebadores (Gravekamp, 1993), que permiten detectar la presencia de ADN de leptospiras patógenas. La técnica molecular utilizada para genotipificar fue multiple-locus variable-number tandem repeats analysis (MLVA). Resultados: se obtuvieron 5 aislamientos de Leptospira spp., de los cuales 2 resultaron positivos a la reacción en cadena de la polimerasa, determinando que se trataba de leptospiras patógenas. Mediante la genotipificación por MLVA se pudo observar que uno de los aislamientos patógenos mostró un patrón correspondiente a la especie Leptospira borgpetersenii, no siendo identificable la otra cepa. Conclusiones: en la ciudad del estudio, que tiene alrededor de 40 000 habitantes, se logró identificar por primera vez una cepa de Leptospira borgpetersenii de fuentes de aguas urbanas, con el peligro potencial que esto representa para la población humana y animal.","palabras_clave":"LEPTOSPIRA BORGPETERSENII|AGUA|SANTA FE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63197,"idioma_id":4,"titulo":"A new non-Mammaliaform Eucynodont from the Carnian-Norian Ischigualasto Formation, northwestern Argentina","resumen":"The record of non-mammaliaformes eucynodonts from the Carnian-Norian Ischigualasto Formation is diverse and abundant, including a medium to large size herbivore and small carnivores. Here is described a new small eucynodont from the Ischigualasto Formation, on the basis of a partial skull. The new taxon is characterized by palatal process of the premaxilla extending posterior to the level of the fi rst postcanine; deep and large maxillary laterodorsal fossa that opens at the level of the root of the upper canine; and postorbital bar diverging posterolaterally at very low angle (35.6°) from the anteroposterior axis of the skull. Results from a phylogenetic analysis supports the new genus placement as a probainognathian eucynodont, more derived than Probainognathus Romer, and more closely related to Ecteninion Martinez, May & Forster and Trucidocynodon Oliveira, Soares & Schultz than to any other eucynodont. Ecteniniidae is proposed as a new clade including the new genus, Ecteninion and Trucidocynodon, and in the phylogenetic hypothesis represents the sister-group of Prozostrodontia (Prozostrodon Bonaparte & Barberena, Tritylodontidae and Mammaliaformes). Additionally, the new taxon from the Ischigualasto Formation shows that the Scaphonyx-Exaeretodon-Herrerasaurus biozone has similar cynodont diversity than the supposedly contemporaneous Hyperodapedon Assemblage Zone of Santa Maria 2 Sequence, in Southern Brazil.","palabras_clave":"ECTENINIIDAE|EUCYNODONTIA|ISCHIGUALASTO|TRIASSIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63198,"idioma_id":5,"titulo":"Primer registro de displasia coxofemoral en un rincosaurio del Triásico Superior","resumen":"La paleopatología es la disciplina que se ocupa de enfermedades evidenciadas en el registro fósil. Este campo abarca la identificación de la patología y las consecuencias que la misma pudo dejar en el animal. En este aporte se presenta el hallazgo de displasia coxo- femoral en un rincosaurio del Triásico Superior, el cual constituye la primera identificación de esta patología en el registro fósil. El ejemplar corresponde a un esqueleto incompleto, parcialmente articulado de Hyperodapedon sanjuanensis (Sill) proveniente de la Formación Ischigualasto (Carniano-Noriano). Los rincosaurios son herbívoros de amplia distribución geográfica, escasa diversidad específica y biocrón acotado a afloramientos triásicos. Las anomalías halladas consisten en atrofia de sacro y pelvis, estructuras esclerosadas en algunas vértebras dorsales y deformación del fémur izquierdo. Estos rasgos son característicos de la displasia acetabular en canes actuales. La displasia acetabular es una enfermedad multifactorial que aparece en los estadios tempranos de desarrollo y se acentúa durante toda la vida, desencadenando una osteoartrosis articular secundaria en el adulto y conduciendo a la invalidez total del miembro afectado. El avanzado estado de la enfermedad del ejemplar estudiado indica un andar denominado \u201Cclaudicación de cuarto grado\u201D, estado en el cual se deja de apoyar el miembro afectado,desplazando el centro de gravedad y cargando el peso del cuerpo sobre las otras patas. Por último, el estado adulto alcanzado por el ejemplar a pesar de la gravedad de su afección, podría sugerir estrategias de supervivencias complejas en Hyperodapedon sanjuanensis, las cuales podrían ser comprobadas en futuros trabajos","palabras_clave":"Triásico|Paleopatología|Displasia|Rincosaurio"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63199,"idioma_id":4,"titulo":"Effect of growth temperature on the high stearic and high stearic-high oleic sunflower traits","resumen":"We investigated variability in the response of oil fatty acid composition to temperature among high stearic andhigh stearic-high oleic sunflower (Helianthus annuus L.) genotypes. Two experiments were conducted with high stearic(including the CAS-3 mutation) and high stearic-high oleic inbred lines (including both the CAS-3 and the high oleic Soldatovmutations). Plants were cultivated in pots with soil, irrigated, and fertilised. Plants were exposed to different day\/nighttemperatures during grain filling: 16\/168C, 26\/168C, 26\/268C, and 32\/268C. Oil fatty acid composition was determined bygas?liquid chromatography in seeds harvested after physiological maturity. Higher temperature during grain filling increasedpalmitic and oleic acid percentages and reduced stearic and linoleic acid percentages, suggesting some modifications onenzymatic activities. When the high oleic mutation was included, the variation in stearic and oleic acid percentages inresponse to temperature was reduced but not the variation in palmitic acid concentration. Variations in fatty acid compositionin high stearic genotypes were mainly associated with night temperature as reported previously for traditional and high oleichybrids. Knowing the effect of temperature on oil fatty acid composition in traditional and mutated genotypes is useful forselecting the environment in which to produce grains with the desired oil quality.","palabras_clave":"grain filling|mutations|oil quality|stearic acid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63200,"idioma_id":5,"titulo":"Intoxicación por plomo y su tratamiento","resumen":"La intoxicación por plomo es un problema de salud pública. La evidencia de poblaciones afectadas por niveles tóxicos de plomo en sangre, confirma que hay que seguir trabajando desde una visión  pluridisciplinar. Es necesario definir políticas para la prevención, detección, diagnostico y tratamiento de los efectos nocivos del plomo sobre la salud. Los niños constituyen el segmento de la población más vulnerable, con consecuencias de alto impacto social, como una disminución del coeficiente intelectual y deficiente desarrollo neurológico. En la práctica clínica, hay cuatro medicamentos que se usan para el tratamiento de la intoxicación crónica, son el edetato cálcico disódico IV e IM, el dimercaprol IM, la penicilamina VO y el succímero VO. Si se tiene en cuenta que sólo penicilamina está autorizada en nuestro país, el problema se torna mayor. Esta breve revisión pretende brindar información sobre la intoxicación por exposición al plomo, los tratamientos recomendados y los disponibles en nuestro país.","palabras_clave":"INTOXICACION|PLOMO|MEDICAMENTOS NO DISPONIBLES|CORDOBA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63201,"idioma_id":4,"titulo":"An approach based on constraint satisfaction problems to disruptive event management in supply chains","resumen":"This work introduces a generalized model for evaluating and restoring feasibility in the execution of supply chain processes. The model was designed to provide automation to the disruption management function of Supply Chain Event Management (SCEM) systems reducing the need for a rescheduling task. This is especially important in collaborative supply chains composed by several partners where the schedules of their corresponding processes must be coordinated. The repair mechanism is based on a constraint satisfaction problem that can be automatically instantiated from self-contained descriptions of the ongoing schedules without previous knowledge of the supply chain structure. In order to provide this ability, we propose a reference model as a common language to describe supply processes and the interaction with the resources in a generic way and focusing on their feasibility aspects. The proposed mechanism intends to make surgical modifications to the current schedule which do not affect the economical and operational considerations made at the moment the schedule was created. On purpose, the allowed changes are limited to the space of slacks already included by the original schedule. This level of repair can be safely delegated to automated systems and would facilitate the design of collaborative inter-organizational business processes to manage events along the supply chain. A complete case study validates the applicability of the proposed models.","palabras_clave":"constraint satisfaction |disruptive event|execution control|supply chain"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63202,"idioma_id":4,"titulo":"Diffusion Dynamics in Pinus sylvestris Kraft Impregnation: Effect of Deacetylation and Galactoglucomannan Degradation","resumen":"During the impregnation stage of a Kraft cooking, dynamic changes occur in wood properties due to the alkali action. Particularly, its ion transport capacity, the effective capillary cross sectional area (ECCSA), is changed due to chemical reactions and swelling. In this work, the ECCSA in the transverse wood direction has been determined for Pinus sylvestris on the basis of the analogy between capillarity and electrical conductivity. Results show that the behavior of ECCSA can be associated with (a) the degree of removal of native acetyl groups and (b) the galactoglucomannan (GGM) losses due to peeling\/stopping reactions and alkaline hydrolysis. Kinetic expressions for both reactions were discussed and a correlation between the ECCSA and both acetyl and GGM contents was established.","palabras_clave":"Diffusion|deacetylation kinetics|galactoglucomannan degradation|effective capillary cross sectional area (ECCSA)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63203,"idioma_id":5,"titulo":"Enfermedades mitocondriales en Córdoba (Argentina). Estudio farmacoepidemiológico","resumen":"Uno de los objetivos de este trabajo es poner en evidenciala cantidad de pacientes con Enfermedades Mitocondriales(EM) bajo tratamiento farmacológico, quese atienden en la ciudad de Córdoba y que retiran medicamentosen el Servicio de Farmacia (SF) del HospitalNacional de Clínicas (HNC). Además, se propone describirlas características demográficas y clínicas de estospacientes, identificar los medicamentos prescriptos,sus pautas de uso y analizar su disponibilidad nacionale internacional.El tipo de estudio fue observacional descriptivo retrospectivo(abril 2010- diciembre 2011; muestreo por conveniencia).La información se tomó de recetas, historiasclínicas y entrevistas a profesionales. Para el análisis dedisponibilidad se consultó a agencias reguladoras denuestro país y otros de referencia.El trabajo farmacoepidemiológico realizado, permitiódar visibilidad a 17 pacientes con EM en Córdoba describiendosus características demográficas y clínicascomo así también los tratamientos farmacológicos empleados.Los medicamentos consistieron, principalmente,en complementos vitamínicos. El 50% de ellosson Medicamentos No Disponibles en nuestro país yalgunos tampoco están disponibles en otros paísesconsultados. Esto permite enfatizar la importancia quetiene su elaboración como medicamentos magistralesdesde el SF del HNC. Dado que no existen datos epidemiológicospublicados sobre los pacientes con EM enCórdoba Argentina, tener un registro de quienes la padecen,aporta resultados que pueden ser el punto departida para investigaciones clínicas más exhaustivas","palabras_clave":"ENFERMEDADES MITOCONDRIALES|MEDICAMENTOS NO DISPONIBLES|FARMACOEPIDEMIOLOGIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63204,"idioma_id":5,"titulo":"PROSPECCIONES ARQUEOLÓGICAS EN EL VALLE DE GUANDACOL, DPTO FELIPE VARELA, LA RIOJA","resumen":"El presente artículo da a conocer los resultados de trabajosde prospección realizados en el valle de Guandacol, dpto. Felipe Varela en eloeste de La Rioja,concretamente sobre ambientes eólico-fluviales en las cuencas del Río Guandacoly La Troya. Elpropósito de este estudio consistió en reunir información de carácterarqueológico y otros rasgos del paisaje, desde una perspectiva regional ydiacrónica en función de comprender la dinámica poblacional de la micro-regióny formas de la ocupación del espacio. Mediante el trazado de transectas serelevaron concentraciones de materiales, fundamentalmente cerámicos, y lugaresde hallazgos aislados. Nos focalizamos y efectuamos análisis espacialesutilizando herramientas proporcionadas por los Sistemas de InformaciónGeográfica (SIG) que nos permitieron evaluar distribuciones, establecerrelaciones entre las locaciones y el paisaje y vinculaciones con la Tambería de Guandacol,núcleo de poblaciones adscriptas al período de los Desarrollos Regionales(1100-1470 d.C.) y de dominación Inca (1470-1536 d.C.). Asimismo profundizamosen los estudios tecnoestilísticos de los conjuntos alfareros que nos dieroncuenta de las variaciones cronoculturales. &nbsp;Palabras claves: Arqueología regional, análisis espaciales,estudios alfareros","palabras_clave":"Análisis|Espaciales|Arqueología|Regional"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63205,"idioma_id":5,"titulo":"Caracterización de la resistencia de Amaranthus quitensis a tres familias de herbicidas","resumen":"Amaranthus quitensis es una especie anual de origen sudamericano que se reproduce por semillas. En Argentina su distribución abarca centro y norte del país y constituye una de las principales malezas en los cultivos de verano (soja, maíz y girasol) del área pampeana. En la región central (Provincia de Córdoba) se detectaron fallas en su control al emplearse herbicidas de las familias de distintas familias (imidazolinonas, sulfonilureas y triazolopirimidinas) por lo cual el objetivo de este trabajo fue caracterizar la resistencia a nivel bioquímico y molecular de biotipos de A. quitensis correspondientes a cuatro localidades de esta región. Específicamente utilizamos distintos herbicidas inhibidores de la Aceto-Lactato Sintasa (ALS), una enzima involucrada en la síntesis de un grupo de aminoácidos, tomando como base de comparación un biotipo susceptible. Como objetivos específicos determinamos el origen geográfico de la resistencia a cada uno de estas tres familias de herbicidas y evaluamos el efecto de diferentes dosis sobre la actividad de la ALS in vitro. Los resultados indican que los materiales analizados corresponden a eventos de resistencia independientes y no a un origen común por movilidad de germoplasma entre las localidades en donde se detectaron las resistencias. Los estudios de variabilidad genética de los biotipos estudiados aportan evidencias de los mecanismos de resistencia frente a diferentes herbicidas que tienen como blanco la enzima ALS.","palabras_clave":"ALS|Amaranthus|resistencia a herbicidas|biodiversidad|agroecosistemas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63206,"idioma_id":4,"titulo":"Birds as mediators of passive restoration during early post-fire recovery","resumen":"Remnant trees or artificial perches in burned areas can favor passive restoration through the perch effect. Birds consuming fruits in remnant vegetation patches or unburned adjacent communities are expected to defecate or regurgitate seeds as they perch on standing burned trees and shrubs in post-fire areas. The purpose of this study was to determine whether standing burned trees and shrubs are used by frugivorous birds as perching structures and if their maintenance promotes passive restoration of burned ecosystems. We: (a) recorded the seed rain of woody species in microsites underneath perches and in the open, (b) recorded seedling survival underneath the canopy of resprouting individuals and in the open, and (c) quantified seed rain and seedling recruitment of endozoochorous species at hectare level. Standing burned trees and shrubs were used as natural perches by frugivorous birds and increased seed arrival into recently burned communities. In addition, seedling survival was greater below the canopy of resprouting woody vegetation than in the open. Thus, standing burned trees and shrubs provide an important structural component attracting frugivorous birds, and therefore seeds during early post-fire regeneration. Resprouting trees and shrubs were also critical during post-fire recovery, because they act as seed traps, facilitate seedling survival and produce fruits that attract frugivorous birds, promoting seed flow among different communities at a landscape level. Therefore, the maintenance of standing burned woody vegetation could favor passive restoration of burned areas. ","palabras_clave":"STANDING BURNED TREES|SEED DISPERSAL|FRUGIVORY|MOBILE-LINKS|SEEDLINGS|SEED RAIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63207,"idioma_id":4,"titulo":"Thermal Parameters and Locomotor Performance in Juvenile of Pleurodema nebulosum (Anura: Leptodactylidae) from the Monte Desert","resumen":"We studied a combination of thermal parameters (critical thermal maximum,selected body temperature, and field body temperature) and locomotorperformance capacities (laboratory and field conditions) of juveniles of Pleurodema nebulosum. We found that field body temperature was determined largely by the temperature of the micro-environment. Field body temperatures of juveniles of P. nebulosum were below selected body temperature. The locomotor performance curve was maximized and reaches a plateau between 30 and 35°C, with 35°C being the temperature at which maximum performance was obtained for analyzed individuals. The plateau values were close to the selected body temperature (Tsel) obtained for the studied frogs. In field conditions the locomotor performance wasdeterminated by the substrate temperature. Apparently, juveniles of P. nebulosum show thermal coadaptation because the selected body temperature and the optimum temperature for locomotion had close values. We believe that the temperatures prevailing during the early hours of activity would allow frogs to explore the micro-environment, covering larger areas in search of food.","palabras_clave":"Field body temperature|Selected body temperature|Critical thermal maximum|Pleurodema nebulosum"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63208,"idioma_id":5,"titulo":"Sentidos de nación y tecnociencia: una mirada comparativa en instituciones del área nuclear","resumen":"Este trabajo pretende aportar al análisis de los nexos entre identidad nacional y tecnociencia. Para ello, estudiaremos los sentidos de nación que se performan en dos ceremonias conmemorativas del cincuentenario de instituciones dedicadas a la producción de ciencia y tecnología en Francia y Argentina: la sede del Commissariat à l Energie Atomique de Grenoble y el Instituto Balseiro, un centro de formación de la Comisión Nacional de Energía Atómica en Bariloche. Mostraremos cómo los sentidos de nación activan y justifican discursos y prácticas ligadas a la producción tecnocientífica concentrándonos en los recursos a través de los cuales la tecnociencia se incorpora en cada celebración. La propuesta se enmarca en investigaciones etnográficas que incluyen, entre sus métodos, la elaboración de entrevistas en profundidad y la observación participante de situaciones cotidianas o extra cotidianas desde una perspectiva comparativa.","palabras_clave":"IDENTIDAD NACIONAL|CONMEMORACION |TECNOCIENCIA|PERFORMANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63209,"idioma_id":5,"titulo":"Relato Hegemónico y Controversias de Relatos: la Memoria en la Discusión de un Modelo de Ciudad","resumen":"Este trabajo aborda el rol de la memoria en la  definición y discusión de la identidad de uno de los territorios tecnocientíficos  e industriales más importantes de Francia: la ciudad de Grenoble. A partir de  entrevistas y fuentes escritas que destacan la tradición del vínculo entre  ciencia, educación superior e industria, analizamos los argumentos del discurso  hegemónico que promueve el modelo grenoblés.  Un modelo que, paralelamente, es cuestionado por colectivos de ciudadanos que  objetan la exclusividad de la orientación tecnocientífica y productiva que se  deriva del modelo y denuncian la convergencia de intereses políticos,  económicos y científicos de las elites locales. Por último, el artículo  reflexiona sobre la&nbsp; ambivalencia  política del discurso de polo tecnocientífico.","palabras_clave":"GRENOBLE|POLO TECNOCIENTIFICO|CONTESTACION|MEMORIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63210,"idioma_id":4,"titulo":"Characterization and structural analysis of the potent antiparasitic and antiviral agent Tizoxanide","resumen":"Tizoxanide [2-(hydroxy)-N-(5-nitro-2-thiazolyl)benzamide, TIZ] is a new potent anti-infective agent which may enhance current therapies for leishmaniasis, Chagas disease and viral hepatitis. The aim ofthis study was to identify the conformational preferences that may be related to the biological activity of TIZ by resolving its crystal structure and characterizing various physicochemical properties, including its experimental vibrational and 13C nuclear magnetic resonance properties, behavior on heating and solubilityin several solvents at 25 C. TIZ crystallizes from dimethylformamide as the carboxamide tautomer in the triclinic system, space group P(1) (No. 2) with the following unit cell parameters at 173(2) K: a = 5.4110(3) Å, b = 7.3315(6) Å, c = 13.5293(9) Å, a = 97.528(3), b = 95.390(4), c = 97.316(5),V = 524.41(6) Å3, Z = 2, Dc = 1.680 g\/cm3, R1 = 0.0482 and wR2 = 0.0911 for 2374 reflections. This modification of TIZ has a \u2018graphitic\u2019 structure and is composed of tightly packed layers of extensively hydrogenbonded molecules. The various spectroscopic data [Diffuse Fourier transform infrared (DRIFT) and FT-Raman, recorded in the range 3600\u2013500 and 4000\u2013200 cm1 respectively, and solid-state 13C NMR]were consistent with the structure determined by X-ray crystallography. From DSC, TG and thermomicroscopy,it was concluded that TIZ is thermally stable as a solid and that melting is not an isolated event from the one-step thermal decomposition that it undergoes above 270 C. This modification of TIZ is practicallyinsoluble in water and slightly soluble in polar aprotic solvents such as dimethylsulfoxide, dimethylformamide and dioxane.","palabras_clave":"CRYSTAL STRUCTURE |13C CPMAS NMR|DRIFT|FT-RAMAN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63211,"idioma_id":4,"titulo":"Environmental fate of glyphosate and aminomethylphosphonic acid in surface waters and soil of agricultural basins","resumen":"Argentinian agricultural production is fundamentally based on a technological package that combines no-till and glyphosate in the cultivation of transgenic crops. Transgenic crops (soybean, maize and cotton) occupy 23 million hectares. This means that glyphosate is the most employed herbicide in the country, where 180 to 200 million liters are applied every year.   The aim of this work is to study the environmental fate of glysophate and its major degradation product, aminomethylphosphonic acid (AMPA), in surface water and soil of agricultural basins. Sixteen agricultural sites and forty-four streams in the agricultural basins were sampled three times during 2012. The samples were analyzed by UPLC-MS\/MS ESI(+\/-) (Aqcuity-Quattro Premier).    In cultivated soils, glyphosate was detected in concentrations between 35 to 1502 µg Kg-1, while AMPA concentration ranged from 299 to 2256 µg Kg-1. In the surface water studied, the presence of glyphosate and AMPA was detected in about 15% and 12% of the samples analyzed, respectively. In suspended particulate matter, glyphosate was found in 67% while AMPA was present in 20% of the samples. In streams sediment Glyphosate and AMPA were also detected in 66% and 88.5% of the samples respectively.   In the present study, it was demonstrated that Glyphosate and AMPA are present in soils under agricultural activity. It was also found that in stream samples the presence of Glyphosate and AMPA is relatively more frequent in suspended particulate matter and sediment than in water.","palabras_clave":"Glyphosate|AMPA|Soil|Surface Water|Sediment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63212,"idioma_id":4,"titulo":"Inhibition of Cytotoxicity of Shiga Toxin of Escherichia coli O157:H7 on Vero Cells by Prosopis alba Griseb (Fabaceae) and Ziziphus mistol Griseb (Rhamnaceae) Extracts","resumen":"The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 mg\/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 mg\/ml for the P. alba aqueous extract and 3.75 mg\/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.","palabras_clave":"SHIGA TOXIN|CYTOTOXICITY|PROSOPIS ALBA GRISEB|ZIZIPHUS MISTOL|ANTIOXIDANT EFFECTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63213,"idioma_id":4,"titulo":"Mechanochemically assisted synthesis of nanocrystalline BiFeO3","resumen":"The synthesis of multiferroic BiFeO3 phase is carried out by means of a solid-state reaction induced by high energy ball-milling between metal chlorides (Lewis´ acids) and sodium hydroxide (base). The chemical reaction occurs at room temperature and produces a solid mixture of nanocrystalline BiFeO3 and NaCl. Thermal treatments at several temperatures are performed to favor the crystallization of the desired phase. The evolution of the mechanochemical reaction and calcination are followed by X-ray diffraction, Raman spectroscopy and scanning electron microscopy. Electric and magnetic ordering transitions are determined by thermal analysis.","palabras_clave":"INORGANIC COMPOUNDS|MAGNETIC MATERIALS|POWDER DIFFRACTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63214,"idioma_id":4,"titulo":"Rapid and reliable identification of Staphylococcus aureus capsular serotypes by means of artificial neural network-assisted Fourier-Transform Infrared Spectroscopy","resumen":"Staphylococcus aureus capsular polysaccharides (CP) are important   virulence factors and represent putative targets for vaccine   development. Therefore, the purpose of this study was to develop a   high-throughput method to identify and discriminate the clinically   important S. aureus capsular serotypes 5, 8, and NT (nontypeable). A   comprehensive set of clinical isolates derived from different origins   and control strains, representative for each serotype, were used to   establish a CP typing system based on Fourier transform infrared (FTIR)   spectroscopy and chemometric techniques. By combining FTIR spectroscopy   with artificial neuronal network (ANN) analysis, a system was   successfully established, allowing a rapid identification and   discrimination of all three serotypes. The overall accuracy of the   ANN-assisted FTIR spectroscopy CP typing system was 96.7% for the   internal validation and 98.2% for the external validation. One isolate   in the internal validation and one isolate in the external validation   failed in the classification procedure, but none of the isolates was   incorrectly classified. The present study demonstrates that ANN-assisted   FTIR spectroscopy allows a rapid and reliable discrimination of S.   aureus capsular serotypes. It is suitable for diagnostic as well as   large-scale epidemiologic surveillance of S. aureus capsule expression   and provides useful information with respect to chronicity of infection.","palabras_clave":"STAPHYLOCOCCUS AUREUS|FTIR|CAPSULE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63215,"idioma_id":4,"titulo":"Spatial and long-term analysis on reference and sewage-impacted sites of the SW Atlantic (38ºS, 57ºW) to assess sensitive and tolerant polychaetes","resumen":"Urbanized littoral areas receive the effluent discharges  producing non-linear changes on benthic organisms. The benthic community  composition is often used into a quality classification to know the  health of the environment. This study undertakes a comparative analysis  of polychaetes associated to mussel beds and environmental variables, in  both reference and sewage-impacted sites, along ten years in a rock  intertidal of the SW Atlantic shore. The results of the study showed  differences in the abundance and dominance of polychaetes spatial and  temporally. A pattern of increasing deterioration of environmental  quality of the area is also shown. This study allowed classifies the  polychaetes in ecological groups in order to calculate environmental  quality indexes.","palabras_clave":"POLYCHAETES|SENSITIVE|TOLERANT|SEWAGE-IMPACT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63216,"idioma_id":4,"titulo":"The dimensionality of ecological networks","resumen":"How many dimensions (trait-axes) are required to predict whether two species interact? This unanswered question originated with the idea of ecological niches, and yet bears relevance today for understanding what determines network structure. Here, we analyse a set of 200 ecological networks, including food webs, antagonistic and mutualistic networks, and find that the number of dimensions needed to completely explain all interactions is small ( < 10), with model selection favouring less than five. Using 18 high-quality webs including several species traits, we identify which traits contribute the most to explaining network structure. We show that accounting for a few traits dramatically improves our understanding of the structure of ecological networks. Matching traits for resources and consumers, for example, fruit size and bill gape, are the most successful combinations. These results link ecologically important species attributes to large-scale community structure.","palabras_clave":"ECOLOGICAL NETWORKS|FOOD WEB STRUCTURE|INTERVALITY|NICHE SPACE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63217,"idioma_id":4,"titulo":"The Insulin-Like Growth Factor 1 Receptor Is Essential for Axonal Regeneration in Adult Central Nervous System Neurons","resumen":"Axonal regeneration is an essential condition to re-establish functional neuronal connections in the injured adult centralnervous system (CNS), but efficient regrowth of severed axons has proven to be very difficult to achieve. Althoughsignificant progress has been made in identifying the intrinsic and extrinsic mechanisms involved, many aspects remainunresolved. Axonal development in embryonic CNS (hippocampus) requires the obligate activation of the insulin-likegrowth factor 1 receptor (IGF-1R). Based on known similarities between axonal growth in fetal compared to mature CNS, wedecided to examine the expression of the IGF-1R, using an antibody to the bgc subunit or a polyclonal anti-peptideantibody directed to the IGF-R (C20), in an in vitro model of adult CNS axonal regeneration, namely retinal ganglion cells(RGC) derived from adult rat retinas. Expression of both bgc and the b subunit recognized by C20 antibody were low infreshly isolated adult RGC, but increased significantly after 4 days in vitro. As in embryonic axons, bgc was localised to distalregions and leading growth cones in RGC. IGF-1R-bgc co-localised with activated p85 involved in the phosphatidylinositol-3kinase (PI3K) signaling pathway, upon stimulation with IGF-1. Blocking experiments using either an antibody whichneutralises IGF-1R activation, shRNA designed against the IGF-1R sequence, or the PI3K pathway inhibitor LY294002, allsignificantly reduced axon regeneration from adult RGC in vitro (,40% RGC possessed axons in controls vs 2\u20138% in thedifferent blocking studies). Finally, co-transfection of RGC with shRNA to silence IGF-1R together with a vector containing aconstitutively active form of downstream PI3K (p110), fully restored axonal outgrowth in vitro. Hence these datademonstrate that axonal regeneration in adult CNS neurons requires re-expression and activation of IGF-1R, and targetingthis system may offer new therapeutic approaches to enhancing axonal regeneration following trauma.","palabras_clave":"IGF-1 receptor|retina ganglion cell|regeneration|axon"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63218,"idioma_id":4,"titulo":"Controlled lateral packing of insulin monolayers influences neuron polarization in solid-supported cultures","resumen":"Neurons are highly polarized cells, composed of one axon and several branching dendrites. One important issue in neurobiology is to understand the molecular factors that determine the neuron to develop polarized structures. A particularly early event, in neurons that do not yet exhibit a discernible axon, is the segregation of activatable IGF-1 (Insulin like Growth Factor-1) receptors in one neurite. This receptor can be activated by insulin in bulk. Nevertheless, it is not known if changes of the organization of insulin as a monomolecular film can affect the induction of neuron polarization. In this work we developed two kind of solid-supported  Langmuir-Blodgett insulin nanofilms. Hyppocampal pyramidal neurons, in early stage of differentiation, were cultured onto the substrates and polarization was studied after 24 hs by confocal microscopy. Also we used surface reflection interference contrast microscopy (SRIC) to study attachment patterns and morphology of growth cones. We observed that insulin nanofilms packed at 14 mN\/m induced polarization in a similar manner to high insulin concentration in bulk. However, insulin nanofilms packed at 44 mN\/m did not induce polarization. Thus, our results provide novel evidence that the neuron polarization through IGF-1 receptor activation can be selectively modulated by the lateral packing of insulin organized as a monomolecular surface for cell growth. ","palabras_clave":"insulin|monolayer|axon|polarity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63219,"idioma_id":4,"titulo":"Regulation of spine density and morphology by IQGAP1 protein domains","resumen":"IQGAP1 is a scaffolding protein that regulates spine number. We now show   a differential role for IQGAP1 domains in spine morphogenesis, in which   a region of the N-terminus that promotes Arp2\/3-mediated actin   polymerization and branching stimulates spine head formation while a   region that binds to Cdc42 and Rac is required for stalk extension.   Conversely, IQGAP1 rescues spine deficiency induced by expression of   dominant negative Cdc42 by stimulating formation of stubby spines.   Together, our observations place IQGAP1 as a crucial regulator of spine   number and shape acting through the N-Wasp Arp2\/3 complex, as well as   upstream and downstream of Cdc42.","palabras_clave":"IQGAP|NEURON|SPINES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63220,"idioma_id":4,"titulo":"Palynofacies analysis and palynology of the Agua de la Mula Member (Agrio Formation) in a sequence stratigraphy framework, Lower Cretaceous, Neuquén Basin, Argentina","resumen":"Variations in thecontent and composition of the palynological organic matter combined with macrofaunalanalysis, contributed to the interpretation of the depositional environmentsconditions of the Agua de la Mula Member at Bajada del Agrio section. Organic-rich AOM-dominated palynofacies arerestricted to the Spitidiscus riccardiizone and the lowermost part of the Crioceratitesdiamantensis zone and correlate with the highest COT values. Terrestrially derived&nbsp;palynofacies characterize the rest of the Agua de la Mula Member and coincidewith lowest COT values (less than 1).&nbsp;The evidence of fluids, like drops and veins,confirm the generation and presumably initial migration of liquid hydrocarboncomponents. ","palabras_clave":"PALYNOFACIES|AGRIO FORMATION|NEUQUEN BASIN|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63221,"idioma_id":4,"titulo":"Behavioral and molecular analysis of nicotine-conditioned place preference in zebrafish","resumen":"                Studies using mice and rats have demonstrated that nicotine  induces a conditioned place preference (CPP), with more effective results  obtained by using biased procedures. Zebrafish have also been used as a model  system to identify factors influencing nicotine-associated reward by using an  unbiased design. Here, we report that zebrafish exhibited putative nicotine  biased CPP to an initially aversive compartment (nicotine-paired group). A  counterbalanced nicotine-exposed control group did not show a significant  preference shift, providing evidence that the preference shift in the  nicotine-paired group was not due to a reduction of aversion for this  compartment. Zebrafish preference was corroborated by behavioral analysis of  several indicators of drug preference, such as time spent in the drug-paired  side, number of entries to the drug-paired side, and distance traveled. These  results provided strong evidence that zebrafish may actually  develop a preference for nicotine, although the drug was administrated in an  aversive place for the fish, which was further supported by molecular studies.  Reverse transcription-quantitative real-time PCR analysis depicted a  significant increase in the expression of &#945;7 and &#945;6 but not &#945;4 and &#946;2 subunits  of the nicotinic receptor in nicotine-paired zebrafish brains. In contrast,  zebrafish brains from the counterbalanced nicotine group showed no significant changes. Moreover, CREB phosphorylation, an indicator of  neural activity, accompanied the acquisition of  nicotine-CPP. Our studies offered an incremental value  to the drug addiction field, because they further describe behavioral features  of CPP to nicotine in zebrafish. The results  suggested that zebrafish exposed to nicotine in an unfriendly environment can  develop a preference for that initially aversive place, which is likely due to  the rewarding effect of nicotine. Therefore, this model can be used to screen  exogenous and endogenous molecules involved in nicotine-associated reward in  vertebrates.   ","palabras_clave":"ZEBRAFISH|NICOTINE|CONDITIONING|BEHAVIOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63222,"idioma_id":4,"titulo":"Wild Pollinators Enhance Fruit Set of Crops Regardless of Honey Bee Abundance","resumen":"Diversity and abundance of wild-insect pollinators have declined in many agricultural landscapes. Whether such declines reduce crop yields, or are mitigated by managed pollinators such as honey bees, is unclear. Here we show universally positive associations of fruit set with wild-insect visits to flowers in 41 crop systems worldwide, and thus clearly demonstrate their agricultural value. In contrast, fruit set increased significantly with visitation by honey bees in only 14% of the systems surveyed. Overall, wild insects pollinated crops more effectively, because increase in their visitation enhanced fruit set by twice as much as an equivalent increase in honey bee visitation. Further, visitation by wild insects and honey bees promoted fruit set independently, so high abundance of managed honey bees supplemented, rather than substituted for, pollination by wild insects. Our results suggest that new practices for integrated management of both honey bees and diverse wild-insect assemblages will enhance global crop yields.","palabras_clave":"APIS MELLIFERA|POLLINATION SERVICE|WILD POLLINATORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63223,"idioma_id":4,"titulo":"Silicophytoliths in Holocene peatlands and fossil peat layers from Tierra del Fuego, Argentina, southernmost South America","resumen":"Phytolith analyses are practically absent from peatlands, except for studies developed on tropical mires and peat profiles. In Tierra del Fuego, Argentina, peatlands represent the largest surface coverage in the South American continent. Because of their continuous sedimentation pattern, they represent relevant palaeoenvironmental records from which the reconstruction of the latest Pleistocene-Holocene history of this region can be obtained. The aim of the study was to analyze the presence of phytoliths in selected mires and fossil peat layers from Tierra del Fuego, Argentina, and to compare these records with present vegetation. Phytoliths from the most abundant and representative plant species, a minerotrophic mire and an alluvial sequence with fossil peat layers were analyzed and described. Nine of the ten plant families analyzed produced phytoliths. The morphotypes described coincided with previous studies carried on species from other regions. Phytolith assemblages from top soil of both sites mostly coincided with the phytolith assemblages of the plants developed over them, except for tabular and cone sedge phytoliths, which were hardly found in soil samples. A lower silicification level and\/or fragmentation processes could account for this absence. Profile analyses showed a low diversity of phytolith morphologies, either due to low plant diversity, a poor phytolith production of the species or to dissolution\/ fragmentation processes. Besides this low diversity, the phytolith assemblages described reflect the presence of grass communities during the development of these environments, being the Pooideae subfamily the dominant component of these communities. This study is the first to present results of phytolith analyses from cold-temperate, subantarctic peatlands, showing the importance of this proxy as a complement of others, such as pollen or charcoal particles in the palaeoenvironmental interpretation of the region.","palabras_clave":"AMORPHOUS SILICA|PHYTOLITHS|PEATLANDS|PLANT COMMUNITIES|SOILS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63224,"idioma_id":4,"titulo":"Amorphous silica biomineralizations in Polytrichum strictum Menzies ex Brid. (Bryophyta)","resumen":"In this study, the presence of amorphous silica biomineralizations (silicophytoliths) in individuals of  Polytrichum strictum collected from Tierra del Fuego, Argentina was analyzed. Gametophyte and  sporophyte samples were subjected to calcination and staining techniques, through which the  biomineralizations obtained were observed and described by optic and scanning electronic microscopes.  Lastly, the composition of them was analyzed by energy dispersive spectroscopy. In both gametophyte  and sporophyte silica skeletons comprising thin elongated silicophytoliths with blunt, round or acute ends  were observed. These morphologies may correspond to the silicification of the costa or midrib region of the  leaves (in the gametophyte) and the seta (in the sporophyte) and to the silicification of the awns of the  leaves. The other morphologies found were silica skeletons comprising silicified walls of tabular polygonal  cells, probably derived from the silicification of the cells of the unistratose lamina of leaves. Energy  dispersive spectroscopy analyses and the use of a phenol-staining technique confirmed the silicon  composition of these biomineralizations. The results obtained comprise the first description of amorphous  silica biomineralization in mosses and the implications of the silicification process in this taxon are  discussed. The data obtained will be relevant not only for anatomical, but also for palaeobotanical studies,  since the preservation of these structures in the soil after the decomposition of organic matter, would allow  the identification of P. strictum in the fossil record.","palabras_clave":"ARGENTINA|MOSSES|PEATLAND|POLYTRICHUM STRICTUM|SILICA|SILICOPHYTOLITHS|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63225,"idioma_id":4,"titulo":"Supramacromolecular organization of gold nanocrystals capped with amphiphilic hyperbranched polyethyleneimine","resumen":"The supramolecular structural aspects of hetero-assemblies constituted of gold nanoparticles capped with amphiphilic unimolecular micelles were studied using synchrotron-based small angle X-ray scattering (SAXS). Experimental results revealed that straightforward transfer of citrate-capped Au nanoparticles from an aqueous environment to a toluenic solution of amphiphilic hyperbranched polymers results in the spontaneous integration of the nanocrystals into the extended hydrophilic domains of  self-assembled supramolecular structures. In this way, we were able to self-organize metal-polymer nanoarchitectures in solution displaying interesting thermoactive functions, i.e.: hybrid assemblies exhibiting negative thermal expansion coefficients. We consider that this strategy has potentiality to realize self-organized supramolecular hetero-assemblies as it provides an alternative methodology to spontaneously integrate nanoscale building blocks into preformed supramolecular objects.","palabras_clave":"Materiales Supramoleculares|Autoensamblado Supramacromolecular|SAXS|Autoorganización"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63226,"idioma_id":5,"titulo":"Estimación de la PPNA y la capacidad de carga por ambientes mediante información satelital en un establecimiento ganadero en la Pampa Deprimida","resumen":"En sistemas de pastoreo extensivo, la productividad primarianeta aérea, PPNA, representa el principal determinante de la capacidad de carga.A escala de paisaje, existe un limitado conocimiento sobre la variaciónespacial y temporal de la PPNA. A partir de información novedosa provista por satélitesse caracterizó la dinámica mensual de la PP NA&nbsp;para el período 2000-2006, cada 5,3 ha en 1000 ha en el centro de la PampaDeprimida. Como resultado, se obtuvo una clasificación donde se diferenciaron 4unidades funcionales. En ellas, la PPNA anual media varió entre los 6700 y 7600kg MS ha año, y el coeficiente de variación interanual entre 12 y 19%. En unaño donde la precipitación fue un 25% menor respecto al promedio, la PPNA disminuyóen promedio un 23% dentro del área evaluada. En cambio, en un año donde la precipitaciónfue un 50% mayor respecto al promedio, la PPNA aumentó un 19%. Contar con este tipode información permitió estimar la variación entre años en la capacidad de carga.En términos absolutos, la capacidad de carga varió entre 0,62 y 1,20 vacas ha, estosignifica un cambio del 30% por encima o&nbsp;por &nbsp;debajo del valor correspondientea un año con precipitaciones promedio.","palabras_clave":"satélite MODIS|seguimiento forrajero"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63227,"idioma_id":4,"titulo":"Basking behaviour in two sympatric herbivorous lizards (Liolaemidae: Phymaturus) from the Payunia volcanic region of Argentina","resumen":"We studied several aspects of the thermal biology of two coexisting saxicolous species of the genus Phymaturus (Liolaemidae) at the Payunia, a volcanic region in Argentina where harsh conditions prevail. We marked individuals in grids and we assessed the environmental conditions when the individuals showed their maximum activity (i.e. when more than 50% of marked individuals were active), the time spent basking, and the postural behaviour in relation to different cloudiness conditions. We found that temperatures recorded when the species exhibit their peaks of activity was higher for P. payuniae, the smaller species, during summer at one of the three studied sites. Phymaturus roigorum exhibited the longest basking periods, but during these longer periods cloudy conditions prevailed. Both species adopted a head-up posture when conditions were sunny and a head-down posture when under cloudy conditions. This is the first contribution where thermal aspects of two sympatric species of Phymaturus are evaluated. ","palabras_clave":"ACTIVITY PEAKS|COEXISTENCE|POSTURAL ADJUSTMENTS|ROCKY LIZARDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63228,"idioma_id":4,"titulo":"Optimal Devaluations","resumen":"The paper analyzes optimal policy in a simple small open economy model with price setting frictions. In particular, the paper studies the optimal response of the nominal exchange rate following a terms-of-trade shock. The paper departs from the New Keynesian (NK) literature in that it explicitly models internationally traded commodities as intermediate inputs in the production of local final goods and assume that the small open economy takes this price as given. This modification is not only in line with the long standing tradition of small open economy models, but also changes the optimal movements in the exchange rate. In contrast with the recent Small Open Economy NK literature, the model in this paper is able to reproduce the comovement between the nominal exchange rate and the price of exports, as it has been documented in the commodity currencies literature. Although the paper shows that there are preferences for which price stability is optimal even without flexible fiscal instruments, the model suggests that more attention should be given to the coordination between monetary and fiscal policy (taxes) in small open economies that are heavily dependent on exports of commodities. The model the paper proposes is a useful framework to study fear of floating.","palabras_clave":"Commodity Prices|New Keynesian|Optimal fiscal and monetary policy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63229,"idioma_id":4,"titulo":"Sexual segregation in timing of foraging by imperial shags (Phalacrocorax atriceps): is it always ladies first?","resumen":"The time seabirds have to forage is restricted while breeding, as time at sea must be balanced against the need to take turns with the partner protecting the nest site or offspring, and timing constraints change once the breeding season is over. Combined geolocator-immersion devices were deployed on eleven Imperial Shags (four males and seven females) in Argentina (43°04´S; 64°2´W) in November 2006 and recovered in November 2007. During the breeding season, females foraged throughout the morning, males exclusively in the afternoon, and variability between individuals was low. Outside the breeding season, both sexes foraged throughout the day, and variability between individuals was high. Timing differences may be explained by higher constraints on foraging or greater demands of parental duties experienced by the smaller sex, females in this case. Sexual differences in reproductive role, feeding habits or proficiency can also lead to segregation in timing of foraging, particularly while breeding. ","palabras_clave":"IMPERIAL CORMORANT|PHALACROCORAX ATRICEPS|SEXUAL SEGREGATION|FORAGING BEHAVIOUR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63230,"idioma_id":4,"titulo":"Molecular population genetics and evolution of the Chagas disease vector Triatoma infestans (Hemiptera: Reduviidae).","resumen":"Triatoma infestans (Klug) is the main vector of Chagas? disease in the Southern Cone of Latin America between the latitudes 10° S and 46° S. The long-term effectiveness of the control campaigns is greatly dependent upon the vector population structure. Mitochondrial DNA (mtDNA) genes have been used in a number of T. infestans population genetic analyses. However, the maternally inherited markers as well as nuclear ribosomal DNA analysed until the present exhibited low or limited levels of variation. Analyses based in microsatellite markers strongly supported the existence of some type of stratification in T. infestans populations and supports the hypothesis of vector population recovery from survivors of the insecticide-treated areas, highlighting the value of population genetic analyses in assessing the effectiveness of Chagas? disease vector control programmes.  Although phylogeographic studies have generally suggested a Bolivian Andean origin of T. infestans, they recovered two reciprocal monophyletic groups of T. infestans and Bolivian populations were not basal as expected for an ancestral group. In addition, a non-Andean origin could not be excluded by mtDNA genealogies that included sylvatic bugs from Gran Chaco. On the other side, mitochondrial and microsatellite markers supported the hypothesis of two independent migration events of colonization and secondary contacts in southern South America. Since the phylogenetic analyses remain inconclusive, more sequences, not only from mitochondrial genes but also from nuclear genes, need to be examined.","palabras_clave":"Chagas' disease vector|DNA sequences|Genetic structure|Microsatellites|Phylogeography|Triatoma infestans"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63231,"idioma_id":4,"titulo":"Vortex kinks in superconducting films with periodically modulated thickness","resumen":"We report magnetoresistance measurements in Nb films having a periodic thickness modulation. The cylinder shaped thicker regions of the sample, which form a square lattice, act as repulsive centers for the superconducting vortices. For low driving currents along one of the axes of the square lattice, the resistivity rho increases monotonously with increasing magnetic field B and the rho-B characteristics are approximately piecewise linear. The linear  rho versus B segments change their slope at matching fields where the number of vortices is an integer or a half integer times the number of protruding cylinders in the sample. Numerical simulations allow us to associate the different segments of linear magnetoresistance to different vortex flow regimes, some  of which are dominated by the propagation of discommensurations (kinks).","palabras_clave":"VORTEX LATTICES|FLUX PINNING|TRANSPORT AND MAGNETIC PROPERTIES |SUPERCONDUCTING FILMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63232,"idioma_id":4,"titulo":"A new chronology for middle Eocene?early Miocene South American Land Mammal Ages","resumen":"Cenozoic South American Land Mammal Ages (SALMAs) have historically been  correlated to the geologic time scale using 40Ar\/39Ar dating and magnetostratigraphy. At Gran Barranca (68.7&#730;W, 45.7&#730;S)\u2014one of South America\u2018s key areas for constraining SALMAs\u2014 existing radioisotopic ages have uncertainties of up to 4 Myr. To better constrain the ages of mammalian assemblages we employed high precision (± <40 kyr) U-Pb dating using single zircon crystals. We dated 9 tuffs from the Sarmiento Formation containing middle Eocene\u2013early Miocene faunas (Barrancan, Mustersan, Tinguirirican, Deseadan, Colhuehuapian and &#8213;Pinturan&#8214;).  The new dates span from 39.861 ± 0.037 Ma to 19.041 ± 0.027 Ma. The La Cancha Tuff, occurring within the Tinguirirican faunal level yielded an age of 33.581 ± 0.015 Ma, confirming that the Vera Member contains the only fossiliferous geologic section encompassing the Eocene\u2013Oligocene Transition in the Southern Hemisphere. The pre-Deseadan fauna, La Cantera is &#8804;30.77 Ma, the age of the Colhuehuapian is expanded to 21.1\u201320.1 Ma, and the Pinturan may be as old as ~19 Ma.  The new U-Pb dates confirm that atmospheric temperatures and vegetation remained constant across the EOT in Patagonia and that hypsodonty occurred in South American ungulates much earlier than on any other continent. Additionally, refinement of the SALMA boundaries will eventually provide the context necessary to compare faunal transitions across continents, although currently too much data are missing to allow such comparisons. Finally, the new ages provide a high-resolution age model from which hypotheses about rates of environmental and evolutionary change at Gran Barranca can be tested.","palabras_clave":"Sarmiento Formation,|magnetostratigraphy|timescale|SALMAs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63233,"idioma_id":4,"titulo":"The two-component systems PrrBA and NtrYX co-ordinately regulate the adaptation of Brucella abortus to an oxygen-limited environment","resumen":"Brucella is the causative agent of the zoonotic disease brucellosis, which is endemic in many parts of the world. The success of Brucella   as pathogen relies in its ability to adapt to the harsh environmental   conditions found in mammalian hosts. One of its main adaptations is the   induction of the expression of different genes involved in respiration   at low oxygen tension. In this report we describe a regulatory network   involved in this adaptation. We show that Brucella abortus&#8197;  PrrBA is a functional two-component signal transduction system that   responds to the redox status and acts as a global regulator controlling   the expression of the regulatory proteins NtrY, FnrN and NnrA, which are   involved in the adaptation to survive at low oxygen tension. We also   show that the two-component systems PrrBA and NtrYX co-ordinately   regulate the expression of denitrification and high-affinity cytochrome   oxidase genes. Strikingly, a double mutant strain in the prrB and ntrY genes is severely impaired in growth and virulence, while the ntrY and prrB single mutant strains are similar to wild-type B.&#8201;abortus. The proposed regulatory network may contribute to understand the mechanisms used by Brucella for a successful adaptation to its replicative niche inside mammalian cells.","palabras_clave":"TWO-COMPONENT|BRUCELLA|DENITRIFICATION|HISTIDINE KINASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63234,"idioma_id":4,"titulo":"Honeybee cognitive ecology in a fluctuating agricultural setting of apple and pear trees","resumen":"Honeybees? plasticity to adjust responses according to environmental changes has been extensively studied in the laboratory but  seldom along temporal variation in natural resources. Apple (Malus domesticus) and pear (Pyrus communis) trees often coexist  in mixed agricultural settings offering different resources, and their blooming periods are slightly shifted. This scenario provides  an opportunity to study how changing environments influence the way the honeybees perceive, learn, discriminate, and use odorresource  information along successive flowering events. We found that honeybees preferred to gather pollen on pear flowers  and nectar on apple. These individual preferences correlated with variations in the type of resources collected at the colony  level according to changes in the floral market. Spontaneous proboscis extension response to pear and apple floral scents of  bees captured at the hives located within the crops fluctuated according to changes in floral availability too. The capability of the  proboscis extension response-trained honeybees to discriminate between both floral scents at the beginning or at the end of the  flowering period was also found, although bees lose this ability when both flowers were fully available. These response patterns  suggest that olfactory information experienced on flowers were memorized to predict reward yet susceptible of being reversed  when the floral scents were no longer available in the orchard. Combining behavioral assays and field observations, we provide  here an example of how honeybees process floral odor information to make decisions about resources in a fluctuating complex  environment.","palabras_clave":"honeybee|ecology|learning|Apis mellifera"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63235,"idioma_id":4,"titulo":"Environmental hyperosmolality regulates phospholipid biosynthesis in the renal epithelial cell line MDCK","resumen":"Hyperosmolality is a key signal for renal physiology. On the one hand, it contributes to the differentiation of renal medullary structures and to the development of the urinary concentrating mechanism. On the other, it is a stress factor. In both cases, hyperosmolality activates processes   that require an adequate extension of cellular membranes. In the present work, we examined whether hyperosmolality regulates phospholipid biosynthesis, which is needed for the membrane biogenesis in the renal epithelial cell line Madin-Darby canine kidney (MDCK). Because phospholipids are the structural determinants of all cell membranes,   we evaluated their content, synthesis, and regulation in   MDCK cultures subjected to different hyperosmotic concentrations of NaCl, urea, or both. Hyperosmolality increased phospholipid content in a concentration-dependent manner. Such an effect was exclusively due to changes in NaCl concentration and occurred at the initial stage of hyperosmolar treatment concomitantly with the expression of the osmoprotective protein COX-2. The hypertonic upregulation of phosphatidylcholine (PC) synthesis, the main constituent of all cell membranes, involved the transcriptional activation of two main regulatory enzymes, choline kinase (CK) and cytidylyltransferase  (CCTalpha ) and required ERK1\/2 activation. Considering that physiologically, renal medullary cells are constantly exposed to high and variable NaCl, these findings could contribute to explaining how renal cells could maintain cellular integrity even in a nonfavorable environment.","palabras_clave":"OSMOTIC STRESS|MEMBRANE BIOGENESIS|RENAL EPITHELIAL CELLS|MADIN-DARBY CANINE KIDNEY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63236,"idioma_id":4,"titulo":"Isothermal sintering kinetic of 3YTZ and 8YSZ cation diffusion","resumen":"Archivo no disponible al momento","palabras_clave":"isothermal sintering kinetic|3YTZ|8YSZ|cation diffusion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63237,"idioma_id":4,"titulo":"Fabrication of dense ZrO2\/CNT composites: Influence of bead-milling treatment","resumen":"Highly concentrated zirconia-carbon nanotube (CNT) water suspensions were prepared using an advanced milling technique. The bead-milling operation parameters were optimized for this system and used to prepare zirconia-stabilized water-based suspensions with different CNT contents. The effects of different milling conditions were studied. The particle dispersion was evaluated by SEM observations on dried suspension. Green?s density and SEM observations of compacts were used to follow the colloidal dispersability of the composites. Materials of tetragonal zirconia and CNTs were prepared with a high concentration of CNTs (1, 5, and 10 wt pct CNT). The homogeneous dispersion and distribution of the fibers in the bulk material after slip casting of the suspension were examined. The samples were sintered using spark plasma sintering (SPS) at 1473 K (1200 _C) and finally, fully dense materials were obtained. The mechanical properties were evaluated using the Vickers indentation technique.","palabras_clave":"Zirconia|Nanotubos de Carbono|Materiales compuestos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63238,"idioma_id":4,"titulo":"Natural therapies assessment for the treatment of endometriosis","resumen":"Study question: Can resveratrol and epigallocatechin-3-gallate (EGCG) inhibit the growth and survival of endometriotic-like lesions in vivo in a BALB\/c model of endometriosis, and in vitro in primary cultures of human epithelial endometrial cells (EECs)?  Summary answer: Resveratrol and EGCG exerted a potent inhibitory effect on the development of endometriosis in a BALB\/c murine model and on the survival of EECs.   What is known already: Endometriosis is a common condition associated with infertility and pelvic pain in women of reproductive age. Resveratrol and EGCG are two polyphenols with anticarcinogenic and antioxidant properties, that have been proposed as natural therapies to treat endometriosis.   Study design, size, duration: Fifty six two-month-old female BALB\/c mice underwent surgical induction of endometriosis¬. Treatments with resveratrol or EGCG started 15 days post-surgery and continued for 4 weeks. Human biopsies were taken with metal Novak curette from posterior uterine wall from 16 patients with untreated endometriosis and 15 controls who underwent diagnostic laparoscopy for infertility.  Materials, setting, methods: After treatments, animals were sacrificed and lesions were counted, measured, excised and fixed. Immunohistochemistry for proliferating cell nuclear antigen (PCNA) and CD34 was performed for cell proliferation and vascularization assessment in lesions. TdT-mediated dUTP Nick-End Labelling (TUNEL) technique was performed for apoptosis evaluation. Peritoneal fluid was collected to analyze vascular endothelial growth factor levels. Human EECs were purified from proliferative phase endometrial biopsies and cultured. The effect of both polyphenols on cell proliferation was determined by a colorimetric assay using the CellTiter 96®AQueous One Solution Cell Proliferation Assay kit and on apoptosis by the TUNEL technique, using an In Situ Cell Death Detection Kit with Fluorescein.  Main results: In the mouse model, both treatments significantly reduced the mean number (p<0.05 vs. Control) and the volume of established lesions (p<0.05 vs. Control). Treatments consistently statistically significantly diminished cell proliferation (resveratrol p<0.01 and EGCG p<0.05, vs. Control), reduced vascular density (resveratrol p<0.01 and EGCG p<0.001, vs. Control) and increased apoptosis within the lesions (resveratrol p<0.01 and EGCG p<0.05, vs. Control). Both compounds induced reduction in human EEC proliferation (p<0.05 vs. Basal) and increased apoptosis (p<0.05 vs. Basal) in primary cultures.   Limitations: In vitro studies were only carried out in epithelial cells from human eutopic endometrium.  Wider implications of the findings: The present findings are promising and will assist the development of novel natural treatments for endometriosis.  Study funding: This study was supported by ANPCYT (PICT 6384 BID 1201 OC-AR) and CONICET (PIP 5471), Argentina.  None of the authors has any conflict of interest to declare.","palabras_clave":"ENDOMETRIOSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63239,"idioma_id":4,"titulo":"Anastrozole and celecoxib for endometriosis treatment, good  to keep them apart?","resumen":"Endometriosis is a benign gynecological disease. Cyclooxygenase (COX)-2 and aromatase proteins have been shown to be overexpressed in eutopic endometrium from women suffering from this disease compared to disease-free women. Furthermore, inhibiting these molecules individually was demonstrated to have antiproliferative and proapoptotic effects both in vitro and in vivo in several models. In this study, the effect of combining celecoxib, a selective COX-2 inhibitor, and anastrozole,an aromatase inhibitor, on the implantation and growth of endometriotic like lesions in a murine model of endometriosis was evaluated. Endometriosis was surgically induced in female BALB\/c mice. After 28 days of treatment with celecoxib, anastrozole or their combination, animals were sacrificed and lesions were counted, measured, excised and fixed. Immunohistochemistry for proliferating cell nuclear antigen and CD34 were performed for assessment of cell proliferation and vascularization. TdT mediated dUTP Nick-End Labelling technique was performed for apoptosis evaluation. Celecoxib was the only treatment to significantly reduce the number of lesions established per mouse, their size and vascularized area. In addition, cell proliferation was significantly diminished and apoptosis was significantly enhanced by both individual treatments. When the therapies were combined they reversed their effects. These results confirm that celecoxib and anastrozole separately decrease endometriotic growth, but when combined they might have antagonizing effects.","palabras_clave":"ENDOMETRIOSIS|COX-2|AROMATASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63240,"idioma_id":4,"titulo":"Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B","resumen":"Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses.  RESULTS:  We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism.  CONCLUSIONS:  IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation.","palabras_clave":"Insulina|IGFII|Insulin receptor|Microscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63241,"idioma_id":4,"titulo":"Quantum Dots as Templates for Self-Assembly of Photoswitchable Polymers: Small, Dual-Color Nanoparticles Capable of Facile Photomodulation","resumen":"A photomodulatable amphiphilic polymer has been synthesized with a backbone of poly[isobutylene-altmaleic anhydride] and pendant dodecyl alkyl chains, Lucifer Yellow (LY) fluorescent probes, and diheteroarylethenes photochromic (PC) groups. The latter serve as reversible UV-activated FRET acceptors for the LY donors. We characterized the spectral and switching properties of the polymer in an organic solvent (CHCl3). In an aqueous medium the polymer forms polymersomes, constituting fluorescence probes &#8764;75 nm in diameter. Self-assembly of the polymer on the surface of a quantum dot (QD) serving as a template creates a dual-color photoswitchable nanoparticle (psNP) with improved properties due to the increase in polymer density and efficiency of PC photoconversion. The psNP exhibits a second QD red emission band that functions as an internal standard requiring only a single excitation wavelength, and is much reduced in size (&lt;20 nm diameter) compared to the polymersomes. The QD template also greatly increases the depth of modulation by photochromic FRET of the LY emission monitored by both steady-state and time-resolved (lifetime) fluorescence (from 20%&#8594;70%, and from 12%&#8594;55%, respectively).","palabras_clave":"Nanoparticles|Photocromism|Fluorescence|FRET"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63242,"idioma_id":4,"titulo":"Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: Similarities with Sunflower chlorotic mottle virus-induced symptom","resumen":"Sugars are part of an integrated redox system, since they are key regulators of respiration and photosynthesis, and therefore of the levels of reducing power, ATP and ROS. These elements are major determinants of the cellular redox state, which is involved in the perception and regulation of many endogenous and environmental stimuli. Our previous findings suggested that early sugar increase produced during compatible Sunflower chlorotic mottle virus (SuCMoV) infection might modulate chlorotic symptom development through redox state alteration in sunflower. The purpose of this work was to characterize redox-related metabolites and gene expression changes associated with high sugar availability and symptom development induced by SuCMoV. The results show that sugar caused an increase in glutathione, ascorbate, pyridine nucleotides and ATP. In addition, higher sugar availability reduced hydrogen peroxide and &#632;PSII. This finding suggests that high sugar availability would be associated with cellular redox alteration and photoinhibitory process. The expression of the genes analyzed was also strongly affected by sugar, such as the down-regulation of psbA and up-regulation of psbO and cp29. The expression level of cytoplasmic- (apx-1 and gr) and chloroplastic- (Fe-sod) targeted genes was also significantly enhanced in sugar-treated leaves. Therefore, all these responses suggest that sugars induce chloroplastic redox state alteration with photoinhibition process that could be contributing to chlorotic symptom development during SuCMoV infection.","palabras_clave":"Compatible plant- virus interaction|Gene expression|Reactive oxygen species|Redox related metabolites|SuCMoV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63243,"idioma_id":4,"titulo":"Cebus nigritus impact the seedling assemblage below their main sleeping sites","resumen":"    Capuchin monkeys (Cebus nigritus) in Argentina  prefer particular sleeping trees they maintain over the years. We compared  species composition and abundance of seedlings under these sites with that of  control sites. Species richness and abundance of seedlings was higher under  sleeping trees than in control plots. Moreover, the proportion of dispersed  versus non-dispersed species and individuals by capuchins was higher under the  most frequently used sleeping trees than in control plots located in other  areas of their home range. Thus, the dominance of plant species dispersed by  these primates in the seedling assemblages below sleeping trees confirm the strong weight of C. nigritus and their potential  influence in the  dynamics of the seedling populations in the forest.    &nbsp;Los  monos capuchinos (Cebus nigritus) en Argentina prefieren árboles dormideros particulares que mantienen durante  varios años. Comparamos la composición y la abundancia de especies de plántulas  debajo de estos sitios con la de sitios controles. La riqueza y abundancia de  especies de plántulas fue mayor debajo de árboles dormideros que en parcelas  controles. Además la proporción de especies e individuos dispersados vs. No dispersados  por los capuchinos fue mayor debajo de los árboles dormideros más  frecuentemente usados que en parcelas controles ubicadas en otras partes de su  área de acción. Así la dominancia de las especies de plantas dispersadas por  estos primates en los ensambles de plántulas debajo de los árboles dormideros  confirma el fuerte peso de C. nigritus y su influencia potencial en las dinámicas de las poblaciones de  plántulas en el bosque.  ","palabras_clave":"Argentina|Cebus|defecation pattern|seedling diversity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63244,"idioma_id":4,"titulo":"Adjoint method for a tumour growth PDE-constrained optimization problem","resumen":"In this paper we present a method for estimating unknown parameters that appear on an avascular, spheric tumor growth model. The model for the tumor is based on nutrient driven growth of a continuum of live cells, whose birth and death generate volume changes described by a velocity field. The model consists of a coupled system of partial differential equations whose spatial domain is the tumor, that changes in size over time. Thus, the situation can be formulated as a free boundary problem. After solving the direct problem properly, we use the model for the estimation of parameters by fitting the numerical solution with real data, obtained via in vitro experiments and medical imaging. We define an appropriate functional to compare both the real data and the numerical solution. We use the adjoint method for the minimization of this functional. &copy; 2013 Elsevier Ltd. All rights reserved.","palabras_clave":"AVASCULAR TUMOR|PDE CONSTRAINED OPTIMIZATION|INVERSE PROBLEM|MATHEMATICAL MODELING|ADJOINT METHOD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63245,"idioma_id":4,"titulo":"Modulatory effects of  Mesorhizobium tianshanense and Glomus intraradices on plant proline and polyamine levels during early plant response of Lotus tenuis to salinity","resumen":"Aims 1-  to evaluate  the effect of Mesorhizobium tianshanense on plant proline and polyamine levels of  Lotus tenuis, and its modulatory effect during plant response to short term salt-stress, and 2- to compare these effects with those caused by mycorrhizal symbiosis.    Methods.  Experiments consisted of a randomized factorial design of two factors:  salinity, (two levels, 0 and 150 mM NaCl) and symbiosis (three levels: uninoculated, Glomus intraradices and M. tianshanense).    Results.  Salinization led to increased proline levels regardless plant organ and symbiotic status, excepting in mycorrhizal L. tenuis roots. Salinity diminished the total polyamine level of control and rhizobial plants, but not in mycorrhizal ones. Variations in the pattern response of the three individual polyamines (putrescine, spermidine and spermine) differed in accordance with the symbiotic status of the plant, highlighting a divergence on proline and polyamine metabolisms between rhizobial and mycorrhizal symbiosis.    Conclusions . Spermidine and spermine  contributed the most with the salt-induced  root polyamine increment observed upon salinization  in roots of nodulated plants, suggesting that these polyamines might mediate  an adaptive role of the  plant-M. tianshanense symbiosis in L. tenuis plants growing in a saline environment.","palabras_clave":"MESORHIZOBIUM TIANSHANENSE|LOTUS TENUIS|PROLINE|POLYAMINES|GLOMUS INTRARADICES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63246,"idioma_id":4,"titulo":"Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana","resumen":"Abstract  AtWRKY30 belongs to a higher plant transcription factor superfamily, which responds to pathogen attack. In previous studies, the AtWRKY30 gene was found to be highly and rapidly induced in Arabidopsis thaliana leaves after oxidative stress treatment. In this study, electrophoretic mobility shift assays showed that AtWRKY30 binds with high specificity and affinity to the WRKY consensus sequence (W-box), and also to its own promoter. Analysis of the AtWRKY30 expression pattern by qPCR and using transgenic Arabidopsis lines carrying AtWRKY30 promoter-&#946;-glucuronidase fusions showed transcriptional activity in leaves subjected to biotic or abiotic stress. Transgenic Arabidopsis plants constitutively overexpressing AtWRKY30 (35S::W30 lines) were more tolerant than wild-type plants to oxidative and salinity stresses during seed germination. The results presented here show that AtWRKY30 is responsive to several stress conditions either from abiotic or biotic origin, suggesting that AtWRKY30 could have a role in the activation of defence responses at early stages of Arabidopsis growth by binding to W-boxes found in promoters of many stress\/developmentally regulated genes.","palabras_clave":"germinación|estrés oxidativo|arabidopsis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63247,"idioma_id":4,"titulo":"EFFECT OF Li- AND Ta- DOPING ON THE FERROELECTRIC PROPERTIES OF NA0.5K0.5NBO3 THIN FILMS PREPARED BY A CHELATE ROUTE","resumen":"The effects of lithium and tantalum doping on the properties of Na0.5K0.5NbO3 (NKN) thin films were investigated. The films were fabricated by an optimized chelate route which offers the advantage of a simple and rapid solution synthesis. The optimization was achieved by investigating the effects of alkaline volatilization loss on film properties. In this way, undoped NKN thin films fabricated by this conventional method exhibited good ferroelectric properties (Pr * 8 lC\/cm2, and Ec * 55 kV\/cm for films annealed at 650 C). The developed chelate route was then used to grow Li (5 %) and Ta (10 %) substituted thin films. Such structures allowed us to compare the effect of these dopant cations on phase formation, microstructureand ferroelectric properties. We show that both modifications produced a remarkable improvement on the ferroelectricity of the films. While the undoped material exhibited large leakage components in films annealed at 600 C, films modified with Li or Ta presented well saturated ferroelectric hysteresis loops, indicating that those ions have a significant influence on the conducting process. The remnant polarizations of the Ta-doped films are greater than those of the Li-doped samples. This feature is however reversed for films annealed at low temperature (600 C) due to the presence of a non-ferroelectric secondary phase in the Ta-doped composition.","palabras_clave":"Ferroelectric thin films|Sol-Gel| Lead-free piezoelectrics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63248,"idioma_id":4,"titulo":"Simple and rapid fabrication of Na0.5K0.5NbO3 thin films by a chelate route","resumen":"Na0.5K0.5NbO3 (NKN) thin films were prepared by a chelate route which offers the advantage of a simple and rapid solution  synthesis. The route is based on the use of acetoin as a chelating agent. The process was optimized by investigating the effects of  alkaline volatilization on film properties.While we observed no evidence of stoichiometry problems due to potassiumvolatilization  loss during the heat treatments, thin films synthesized with insufficient sodium excess presented a potassium-rich secondary  phase, which has a significant influence on the ferroelectric properties. We show that the amount of spurious phase decreases  with increasing Na+ concentration, in such a way that a 20mol% Na+ excess is necessary to fully compensate the volatilization loss  that occurred during the heat treatment. In this way, NKN thin films annealed at 650&#8728;C presented a well-crystallized perovskite  structure, no secondary phases, well-defined ferroelectric hysteresis loops (&#119875;&#119903; &#8764; 9&#120583;C\/cm2, &#119864;&#119862; &#8764; 45kV\/cm), and low leakage  current density (2 × 10&#8722;7 A\/cm2 at 80 kV\/cm).","palabras_clave":"NKN thin films|Chelate route"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63249,"idioma_id":4,"titulo":"Functional human sperm capacitation requires both bicarbonate-dependent PKA activation and down-regulation of Ser\/Thr phosphatases by Src family kinases","resumen":"In all mammalian species studied  so far, sperm capacitation correlates with an increase in protein tyrosine  (Tyr) phosphorylation mediated by a bicarbonate-dependent cAMP\/PKA pathway. Recent  studies in mice revealed however that a Src Family Kinase (SFK) induced  inactivation of serine\/threonine (Ser\/Thr) phosphatases is also involved in the  signaling pathways leading to Tyr phosphorylation. In view of these observations and  with the aim of getting a better understanding of the signaling pathways  involved in human sperm capacitation, in the present work we investigated the  involvement of both the cAMP\/PKA and SFK\/phosphatase pathways in relation to  the capacitation state of the cells. For this purpose, different signaling events and  sperm functional parameters were analyzed as a function of capacitation  time.  Results revealed a very early  bicarbonate-dependent activation of PKA indicated by the rapid (1 min) increase  in both phospho-PKA substrates and cAMP levels (p&lt;0.05). However, a complete pattern of Tyr phosphorylation was  detected only after 6 h-incubation at which time sperm exhibited the ability to  undergo the acrosome reaction (AR) and to penetrate zona-free hamster eggs.  Sperm capacitated in the presence of the SFK inhibitor SKI606 showed a decrease  in both PKA substrate and Tyr phosphorylation levels which was overcome by  exposure of sperm to the Ser\/Thr phosphatase inhibitor okadaic acid (OA). However,  OA was unable to induce phosphorylation when sperm were incubated under  PKA-inhibitory conditions (i.e. in the absence of bicarbonate or presence of  PKA inhibitor). Moreover, the increase in PKA activity by exposure to a cAMP  analogue and a phosphodiesterase inhibitor did not overcome the inhibition  produced by SKI606. Whereas the presence of SKI606 during capacitation produced  a negative effect (p&lt;0.05) on  sperm motility,  progesterone-induced AR and fertilizing ability, none of these inhibitions were  observed when sperm were exposed to SKI606 and OA. Interestingly, different  concentrations of inhibitors were required to modulate human and mouse  capacitation revealing the species-specificity  of the molecular mechanisms underlying this process. In conclusion, our results  describe for the first time the involvement of both PKA activation and Ser\/Thr  phosphatase down-regulation in functional human sperm capacitation and provide  convincing evidence that early PKA-dependent phosphorylation is the convergent regulatory  point between these two signaling pathways.","palabras_clave":"sperm capacitation|src|kinases|fertilization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63250,"idioma_id":4,"titulo":"Picoplankton abundance and cytometric group diversity along a trophic and latitudinal lake gradient","resumen":"The picoplankton (PP) is responsible for major ecosystem functions in most aquatic environments. However, not much is known about the factors that regulate its total abundance and community structure. Using the capability of flow cytometry (FC) to detect particles based on their pigments and nucleic-acid content, we described and quantified the photosynthetic (PPP) and the heterotrophic (HB: heterotrophic bacteria) populations (or groups) composing PP in 32 water bodies located along a trophic and latitudinal gradient in the Argentinean Patagonia to determine flow-cytometrically defined community structures. We set to identify the environmental variables regulating total PP abundance, group structure and cytometric diversity. We identified a total of 28 different cytometric populations within the HB, 14 of phycoerythrin (PE)-rich picocyanobacteria (Pcy), 8 of phycocyanin (PC)-rich Pcy, and 41 of picoeukaryotes (Peuk) in the different water bodies, with average 3.9 HB and 4.6 PPP groups per water body. We found a strong influence of environmental factors and a less marked effect of latitude on PP structure. HB and PPP abundances decreased towards higher latitudes but their cytometric diversity did not, whereas HB, PC-rich Pcy and Peuk abundances together with PPP diversities increased with higher values of chlorophyll. The relative contribution of PE-rich Pcy to total Pcy decreased with chl a, whereas the relative contribution of PC-rich Pcy and the number of PC-rich cytometric populations increased with chl a. Peuk prevailed over Pcy with increasing trophic status and light attenuation, whereas HB prevailed over PPP with increasing trophy.","palabras_clave":"FLOW CYTOMETRY|PICOPLANKTON|PATAGONIAN WATER BODIES|LATITUDINAL GRADIENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63251,"idioma_id":4,"titulo":"Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina)","resumen":"We assessed the influence of environmental factors in shaping the free-living bacterial community structure in a set of shallow lakes characterized by contrasting stable state patterns (clear-vegetated, inorganic-turbid and phytoplankton-turbid). Six temperate shallow lakes from The Pampa Plain (Argentina) were sampled over an annual cycle, and two fingerprinting techniques were applied: A 16S rDNA analysis was performed using DGGE profiles, and a 16S- 23S internally transcribed spacer region (ITS) analysis was conducted by means of ARISA profiles. Our results show that the steady state that characterized the different shallow lakes played a major role in structuring the community: The composition of free- living bacteria differed significantly between clear-vegetated, inorganic-turbid and phytoplankton-turbid shallow lakes. The state of the system was more important in determining these patterns than seasonality, geographic location, or degree of hydrological connectivity. Moreover, this strong environmental control was particularly evident in the pattern observed in one of the lakes, which  shifted from a clear to a turbid state over the course of the study. This lake showed a directional selection of species from a typical clear-like to a turbid-like community. The combined DGGE\/ARISA approach revealed not only broad patterns among different alternative steady states, but also more subtle differences within different regimes.","palabras_clave":"BACTERIOPLANKTON|SHALLOW LAKES|DGGE|ARISA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63252,"idioma_id":4,"titulo":"SSA study of early polyethylenes degradation stages. Effects  of attack rate, of average branch length, and of backbone polymethylene sequences length distributions","resumen":"Low-density polyethylenes have been attacked by free radicals in the presence of oxygen to promotechains scission, aiming at showing effects of the molecular structure on the thermal behavior asdetermined by using a Successive Self-Nucleation and Annealing (SSA) technique. Three types of lowdensitypolyethylenes were used: a hydrogenated polybutadiene (HPB), as a model for a statistical linearethyleneebutene copolymer, a linear ethyleneebutene copolymer (LLDPE) with short branches notrandomly distributed along the main chains, and a low-density polyethylene (LDPE) with randomlyplaced long and short branching. All the polymers were subjected to thermal oxidation in air. Additionally,the LDPE and HPB were exposed to gamma irradiation in air, while the HPB was also degradedwith an organic peroxide. By virtue of the thermally activated oxidation, the three polymers show signsof molecular scission evidenced by the appearance of endotherms in the high temperature range of thefinal SSA thermogram. These endotherms are associated with the melting of thicker crystals formed bylonger polymethylene sequences that were not available for crystallization in the original polymer.Similar indication of chain scission was detected in some of the irradiated HPB. In the case of the irradiatedLDPE or the HPB modified with peroxide, the main effect observed was crosslinking that wasidentified by a reduction of the area of the higher melting endotherms in the final SSA thermogram.","palabras_clave":"ETHYLENE-BUTENE COPOLYMERS|RADIATION|CROSSLINKING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63253,"idioma_id":4,"titulo":"The humerus of South American caviomorph rodents: shape, function and size in a phylogenetic context","resumen":"South American caviomorph rodents comprise four major lineages encompassingwide taxonomic and ecological diversity, but the morphological diversity of theirpostcranial skeleton has not been thoroughly explored using phylogenetic comparativemethods. The main goal of this work is to analyze their humerus usinggeometric morphometrics in a phylogenetic context and attempt to tease apart theinfluence of locomotory preferences and shared evolutionary history on morphologicalvariation. We examined 28 genera in 9 families representing all majorclades. Humeral shape was captured by 13 landmarks and four semilandmarks in2D. In the morphospace of the first two principal components, most taxa werelocated near the origin along both axes. Fossorial octodontoids were apart fromthis central group. Most caviids were separated in one extreme of the morphospace;the specialized digging ctenomyid Ctenomys, the fossorial chinchillid Lagostomusand the generalized cavioid Cuniculus were located at the opposite end.Phylogenetic signal was significant. Regressions of shape on size were not significant;regression of shape on habit was significant for raw data and not significantafter phylogenetic control. Humeral shape variation was primarily associated withthe phylogenetic structure of the group; additionally, some morphological traitswere associated with particular habits and interpreted as functional specializations.This association between humeral shape and both phylogeny and habit atdifferent hierarchical levels suggests early ecomorphological diversification ofcaviomorphs","palabras_clave":"FUNCTIONAL MORPHOLOGY|GEOMETRIC MORPHOMETRICS|HUMERUS|PHYLOGENETIC COMPARATIVE METHODS|SOUTH AMERICAN HYSTRICOMORPHS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63254,"idioma_id":4,"titulo":"Protective effect of oestradiol in the coeliac ganglion against ovarian apoptotic mechanism on dioestrus","resumen":"The aims of this work were to investigate if oestradiol 10&#8722;8M in the incubation media of either the ovary alone (OV) or the ganglion compartment of an ex vivo coeliac ganglion?superior ovarian nerve?ovary  system (a) modifies the release of ovarian progesterone (P4) and oestradiol (E2) on dioestrus II, and (b)modifies the ovarian gene expression of 3-HSD and 20-HSD enzymes and markers of apoptosis. The  concentration of ovarian P4 release was measured in both experimental schemes, and ovarian P4 and E2 in the ex vivo system by RIA at different times. The expression of 3-hydroxysteroid dehydrogenase,20-hydroxysteroid dehydrogenase and antiapoptotic bcl-2 and proapoptotic bax by RT-PCR were determined. E2 added in the coeliac ganglion caused an increase in the ovarian release of the P4, E2 and 3-HSD,while in the ovary incubation alone it decreased P4 and 3-HSD but increased and 20-HSD and bax\/bcl-2  ratio. It is concluded that through a direct effect on the ovary, E2 promotes luteal regression in DII rats, but the addition of E2 in the coeliac ganglion does not have the same effect. The peripheral nervous system,through the superior ovarian nerve, has a protective effect against the apoptotic mechanism on DII.","palabras_clave":"OESTRADIOL|COELIAC GANGLION |APOPTOSIS |DIOESTRUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63255,"idioma_id":4,"titulo":"The impact of obesity on male reproduction: its biological significance","resumen":"SUMMARY Since obesity and male subfertility have increased in parallel during the last decades, there might exist an association between these two phenomenons; this hypothesis has been explored by several researchers. Although there is no consensus, it is apparent that obesity may impact men´s reproductive potential by several mechanisms that include alterations on the hypothalamus-pituitary-testicular axis physiology, modifications on spermatogenesis and semen quality and\/or impairment of men´s sexual health. This review intends to summarize the underlying bases of such alterations and propose some new ones, without miscalculating their biological significance. Evidences demonstrated that obesity is not rigorously related to subfertility and currently researchers are exploring the existence of a genetic predisposition to obesity-linked sterility. Nonetheless the impact of obesity on male reproductive potential must be fully elucidated, because the prevalence of obesity is growing and consequently the number of obese men with reduced fertility will rise as well. ","palabras_clave":"epididymal maturation|epididymis|spermatogenesis|overweight"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63256,"idioma_id":5,"titulo":"El campo religioso argentino hoy: creencia, autoadscripción y práctica religiosa. Una aproximación a través de datos agregados","resumen":"El artículo se pregunta por la vigencia y las formas diversas que tomó el legado cristiano de la sociedad argentina, así como sobre las configuraciones en torno a nuevos actores. Efectuamos una aproximación a la situación religiosa del país recurriendo al análisis de datos recogidos por encuestas realizadas a muestras representativas de todo el país; utilizando principalmente los datos generados por el Barómetro de la Deuda Social de la UCA. Primero discutimos las limitaciones de emplear este tipo de estrategia metodológica y las precauciones que requiere este uso para describir el campo religioso en Argentina. Luego efectuamos una aproximación al alcance de la creencia en Dios; continuamos con la caracterización de la afiliación\/identidad\/adscripción religiosa; y luego abordamos la práctica y participación religiosa. Para todas estas dimensiones analizamos en qué medida y cómo son influidas por el sexo, la edad, el nivel de instrucción, la pobreza estructural y la región del país. El trabajo destaca que la herencia cristiana de la sociedad es el sustrato desde el cual las personas -solas, en grupos, y desde grados de cercanías institucionales muy variadas- se referencian de múltiples maneras para construir sus proyectos de vida, su configuración de sentidos, su mundo simbólico, su ritualidad.","palabras_clave":"CAMPO RELIGIOSO ARGENTINO|CREENCIA RELIGIOSA|PRACTICA RELIGIOSA|AUTOADSCRIPCION RELIGIOSA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63257,"idioma_id":4,"titulo":"Differential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide","resumen":"Acetohydroxyacid synthase (AHAS) catalyzes the first reaction in branch chain amino acids biosynthesis.This enzyme is the target of several herbicides, including all members of the imidazolinone family. Littleis known about the expression of the three acetohydroxyacid synthase genes (ahas1, ahas2 and ahas3) in sunflower. The aim of this work was to evaluate ahas gene expression and AHAS activity in different tissues of sunflower plantlets. Three genotypes differing in imidazolinone resistance were evaluated, two ofwhich carry an herbicide resistant-endowing mutation known as Ahasl1-1 allele. In vivo and in vitro AHAS activity and transcript levels were higher in leaves than in roots. The ahas3 transcript was the less abundant in both tissues. No significant difference was observed between ahas1 and ahas2 transcript levels ofthe susceptible genotype but a higher ahas1 transcript level was observed in leaves of genotypes carryingAhasl1-1 allele. Similar transcript levels were found for ahas1 and ahas2 in roots of genotypes carrying Ahasl1-1 allele whereas higher ahas2 abundance was found in the susceptible genotype. Herbicide treatment triggered tissue-specific, gene and genotype-dependent changes in ahas gene expression. AHASactivity was highly inhibited in the susceptible genotype. Differential responses were observed between in vitro and in vivo AHAS inhibition assays. These findings enhance our understanding of AHAS expressionin sunflower genotypes differing for herbicide resistance and its response to herbicide treatment.","palabras_clave":"Helianthus annuus L.|Imidazolinone herbicide|ahas gene expression|AHAS activity|Herbicide tolerance"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63258,"idioma_id":4,"titulo":"Lipoic acid prevents liver metabolic changes induced by administration of a fructose-rich diet","resumen":"Background: Fructose administration rapidly induces oxidative stress that triggers compensatory hepatic metabolic changes. We evaluated the effect of an antioxidant, R\/S-&#945;-lipoic acid on fructose-induced oxidative stress and carbohydrate metabolism changes.  Methods: Wistar rats were fed a standard commercial diet (control), the same diet plus 10% fructose in drinking water (fructose), or injected with R\/S-&#945;-lipoic acid (35 mg\/kg, i.p.) (control+L and fructose+L). Three weeks thereafter, blood samples were drawn to measure glucose, triglycerides, insulin, and the homeostasis model assessment-insulin resistance (HOMA-IR) and Matsuda indices. In liver, we measured gene expression (real-time PCR), protein content (Western blot) and activity of several enzymes, and metabolite concentration.   Results: Comparable body weight changes and calorie intake were recorded in all groups after the treatments. Fructose fed rats had hyperinsulinemia, hypertriglyceridemia, higher HOMA-IR and lower Matsuda indices compared to control animals. Fructose fed rats showed increased fructokinase gene expression, protein content and activity, glucokinase and glucose-6-phosphatase gene expression and activity, glycogen storage, glucose-6-phosphate dehydrogenase mRNA and enzyme activity, NAD(P)H oxidase subunits (gp91phox and p22phox) gene expression and protein concentration and phosphofructokinase-2 protein content than control rats. All these changes were prevented by R\/S-&#945;-lipoic acid co-administration.   Conclusions: Fructose induces hepatic metabolic changes that presumably begin with increased fructose phosphorylation by fructokinase, followed by adaptive changes that attempt to switch the substrate flow from mitochondrial metabolism to energy storage. These changes can be effectively prevented by R\/S-&#945;-lipoic acid co-administration.   General Significance: Control of oxidative stress could be a useful strategy to prevent the transition from impaired glucose tolerance to type 2 diabetes.","palabras_clave":"Glucose metabolism |Glycoxidative stress|R\/S-&#945;-lipoic acid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63259,"idioma_id":4,"titulo":"Within-population variability in architectural traits and suitability to forestry conditions in Nothofagus nervosa (= N. alpina; Nothofagaceae)","resumen":"There is evidence that Nothofagus nervosa (= N. alpina) is suitable for timber production in temperate regions due to its wood quality and fast growth. However, high intra-specific variability in the architectural traits of this species limits its usefulness. & Aims This study was aimed at evaluating intra-specific variability in the architecture of N. nervosa trees at the population level, with emphasis on traits related to the suitability of trees for timber production. & Methods The size of 13-year-old trees installed in an experimental field population and the extent of differentiation between trunk and branches were compared among families (trees derived from different mother trees). & Results The large majority of trees exhibited architectural features indicative of high suitability for timber production: clear differentiation between trunk and main branches and few occurrences of permanent trunk forks, apex deaths, and sylleptic branching. Trees belonging to different families differed in height and trunk diameter. Micro-environmental conditions had a very significant effect on most architectural traits, including the trunk differentiation. & Conclusions N. nervosa emerges as a suitable option for timber production in temperate regions. Trunk growth seems to be under both environmental and genetic control in this species.","palabras_clave":"ARCHITECTURE|DOMESTICATION|HIERARCHICAL STRUCTURE|GROWTH UNIT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63260,"idioma_id":4,"titulo":"Analysis of sequences of hepatitis C virus NS5A genotype 1 in HIV-coinfected patients with a null response to nitazoxanide or peg-interferon plus ribavirin","resumen":"Even though new drugs have been approved for HCV treatment, the risk for drug-drug interactions and the concern over overlapping toxicities has hindered the development of studies in HIV\/HCV coinfected individuals. Traditional treatment with pegylated interferon plus ribavirin (peg-IFN+RBV) is highly expensive and has low rates of sustained virological response in co-infected patients especially if infected with HCV genotype 1. Nitazoxanide (NTZ) is a drug that is being evaluated for the treatment of chronic HCV infection both in HCV monoinfected and HIV\/HCV coinfected patients. To understand NTZ resistance mechanism could allow minimizing the development of resistance and expanding the treatment options mainly in special populations such as HIV\/HCV coinfected patients.  Similarly to IFN, NTZ increases the activity of the cellular protein kinase activated by double-stranded RNA (PKR), a key kinase in the innate antiviral response. In order to elucidate whether sequence heterogeneity in the PKR-binding domain of the HCV genotype 1 NS5A protein could influence the antiviral activity of either NTZ monotherapy or, peg-IFN+RBV, baseline and end-of-therapy plasma samples from two groups of eleven non-responder HIV\/HCV coinfected patients that received NTZ or peg-IFN+RBV were studied. Most of the HCV NS5A sequences examined at end-of-therapy did not change from baseline, even after 30 days course of antiviral therapy. An extensive comparison with database HCV-NS5A genotype 1 and 4 sequences with reported IFN therapy outcome was performed in order to infer their phylogenetic relationships.  The HCV genotype 1 NS5A nucleotide sequences from therapy non-responder patients were intermingled amongst those the database, irrespective of their IFN-therapy outcome. When comparing the NS5A-PKRBD amino acid identity, it was only significantly different against those sequences ascribed to genotype 4 but not to those of genotype 1 (p0.05, respectively). In conclusion, despite both IFN and NTZ sharing the protein kinase activated by double-stranded RNA as the cellular target, the HCV genotype 1 strategy to counteract the IFN action mediated by NS5A ISDR\/PKRBD does not explain drug resistance in HIV\/HCV coinfected patients. Other plausible viral factors involved are discussed as well.","palabras_clave":"nitazoxanide|HCV|genotype 1| HIV coinfection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63261,"idioma_id":4,"titulo":"Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA","resumen":"The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM\/ATR, Chk1\/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies.","palabras_clave":"p19INK4d|DNA repair|Cell cycle|Chromatin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63262,"idioma_id":5,"titulo":"Subgrupo Río Colorado (Grupo Neuquén): registros de paleosismidad en la cuenca de antepaís andina, Cuenca Neuquina, Provincias de Neuquén y Río Negro, Argentina.","resumen":"The Neuquén basin is located in the central and western part of Argentina and represents the most important Mesozoic-Neogene  depocenter in the country and is one of the basins of the Southern Hemisphere with an excellent marine and continental record.  It is characterized by three stage of evolution: an initial rift, subduction related to \u201Csag\u201D a state of thermal and foreland basin. The  Río Colorado Subgroup is deposited during the foreland development. Subgroup sindepositacional tectonics is recorded by three  deformational levels, two in Bajo de la Carpa Formation and one in the Anacleto Formation. They are composed of deformation and  liquefaction structures interpreted as sismites. The deformation is represented by simple load structures, balls and pillows, flaming,  pseudonódulos, lamination convolute, pinch and swell, slumps, sandstone disorganized, folded sandstone, mud diapirs, fractures,  dish and pillar and autoclastic breccias. These deformational structures in the Bajo de la Carpa Formation involves deposits of channel  belts and flood plain, while in the Anacleto Formation deposits affects the tidal channels of high and low sinuosity, intertidal  mud flats, sandy and muddy plain supratidal associated with an middle estuarine system. The recognized sismitas levels are probably  associated with Sierra Barrosa and Lindero Atravesado lineaments and these maybe are dynamic response to the activity of the structures.  The nature of the structures and their distribution suggests that the magnitude of earthquakes is greater than 6 on Richter scale.","palabras_clave":"SEDIMENTOLOGÍA|DEFORMACIONES|SISMITAS|CUENCA NEUQUINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63263,"idioma_id":4,"titulo":"Elevated Serum Levels of Macrophage Migration Inhibitory Factor Are Associated with Progressive Chronic Cardiomyopathy in Patients with Chagas Disease","resumen":"Clinical symptoms of chronic Chagas disease occur in around 30% of the individuals infected with Trypanosoma cruzi and  are characterized by heart inflammation and dysfunction. The pathogenesis of chronic chagasic cardiomyopathy (CCC) is  not completely understood yet, partially because disease evolution depends on complex host-parasite interactions.  Macrophage migration inhibitory factor (MIF) is a pleiotropic proinflammatory cytokine that promotes numerous  pathophysiological processes. In the current study, we investigated the link between MIF and CCC progression. Immunohistochemical  analysis demonstrated MIF overexpression in the hearts from chronically T. cruzi-infected mice, particularly  those showing intense inflammatory infiltration. We also found that MIF exogenously added to parasite-infected murine  macrophage cultures is capable of enhancing the production of TNF-a and reactive oxygen species, both with pathogenic  roles in CCC. Thus, the integrated action of MIF and other cytokines and chemokines may account for leukocyte influx to the  infected myocardium, accompanied by enhanced local production of multiple inflammatory mediators. We further  examined by ELISA the level of MIF in the sera from chronic indeterminate and cardiomyopathic chagasic patients, and  healthy subjects. CCC patients displayed significantly higher MIF concentrations than those recorded in asymptomatic T.  cruzi-infected and uninfected individuals. Interestingly, increased MIF levels were associated with severe progressive Chagas  heart disease, in correlation with elevated serum concentration of high sensitivity C-reactive protein and also with several  echocardiographic indicators of left ventricular dysfunction, one of the hallmarks of CCC. Our present findings represent the  first evidence that enhanced MIF production is associated with progressive cardiac impairment in chronic human infection  with T. cruzi, strengthening the relationship between inflammatory response and parasite-driven pathology. These  observations contribute to unravel the elements involved in the pathogenesis of CCC and may also be helpful for the design  of novel therapies aimed to control long-term morbidity in chagasic patients","palabras_clave":"MACROPHAGE MIGRATION INHIBITORY FACTOR|INFLAMMATORY CARDIOMYOPATHY|CHRONIC CHAGAS DISEASE|PATHOGENESIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63264,"idioma_id":5,"titulo":"El peronismo y las elecciones bonaerenses. De la derrota a la consolidación en el gobierno provincial, 1983-1991","resumen":"Este artículo analiza las performances electorales del peronismo bonaerense entre 1983 y 1991. Intenta comprender las causas que incidieron en que, tras ser derrotado en las primeras elecciones, recuperara la primacía en 1987 y se consolidara como partido en el gobierno. Indaga acerca de la evolución del voto justicialista en la provincia en elecciones de gobernador (1983, 1987 y 1991) y parlamentarias (1985 y 1989), aplicando inferencia ecológica. Describe tendencias referidas a la evolución del padrón y la participación electoral, la distribución seccional y la expansión territorial del voto peronista. Además, relaciona las conclusiones obtenidas del análisis cuantitativo con los procesos históricos. Brinda algunos indicios que complejizan las explicaciones en torno a los fenómenos político-electorales y contribuye a entender en su especificidad la historia política bonaerense del período reciente.","palabras_clave":"performances electorales del peronismo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63265,"idioma_id":4,"titulo":"Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)","resumen":"A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.","palabras_clave":"SuCMoV|Sunflower|Infectious clone|Potyvirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63266,"idioma_id":4,"titulo":"The Birth of Paleomagnetism in Latin America: Argentina","resumen":"Paleomagnetic activities in Latin America started with the first field trip of the young Ken Creer in 1957. In the early sixties, and by initiative of the by then Director of the Department of Geological Sciences at the University of Buenos Aires, Feliz Gonzalez Bonorino; the engineer Daniel A. Valencio is hired to start the teaching of Geophysics to the undergraduate students of Geology and to begin with the Paleomagnetic research in Argentina. The Paleomagnetic Laboratory of Buenos Aires was created in 1964 and rapidly became an internationally recognized center, promoter of research in the emergent theory of Plate Tectonics and a spreading center of Paleomagnetism in Latin America. The network of friends across the continent and the unselfish cooperation among colleagues from different Latin American countries helped in building up the first laboratories in Brazil and Mexico. Despite several vicissitudes that conspired against its survival along the last five decades, the Paleomagnetic Laboratory \u201CDaniel A. Valencio\u201D continues in full activity honoring the tradition of unselfish and friendly collaboration that shaped its beginnings.","palabras_clave":"PALEOMAGNETISM|PALEOMAGNETIC RESEARCH|PALEOMAGNETIC LABORATORIES|HISTORY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63267,"idioma_id":4,"titulo":"Performance of alginate films for retention of l-(+)-ascorbic acid","resumen":"In view of acting as controlled delivery systems for nutritional supplementation, therapy or antioxidant activity at interfaces, alginate films of different copolymer composition and glycerol plasticizer levelswere developed in the presence of Ca2+ for achieving higher stability of l-(+)-ascorbic acid (AA). The ability of the alginate network to preserve AA from hydrolysis, tested by storage under vacuum at 25 &#9702;C, only decreased with the relative humidity (RH) increase when alginates were mainly constituted by guluronic\u2013guluronic acid blocks (GG), whereas also decreased with the glycerol level increase when mannuronic\u2013mannuronic acid (MM) and\/or alternating guluronic\u2013mannuronic (GM + MG) flexible blocks were present in higher proportions. This result could be probably related to the lower capability of the latter alginate block compositions to immobilize water in the network as they are not able to constitute Ca2+ mediated junction zones where water molecules are highly retained. Films also studied under air storage showed that even at less favorable conditions of RH and glycerol levels, both GG and GM + MGenriched alginate networks in general preserved AA from oxidation. It also demonstrated that hydrolysis is the principal way by which AA is lost when supported in films.","palabras_clave":"ALGINATE FILMS|ASCORBIC ACID HYDROLYSIS|GLYCEROL|BIOMOLECULE DELIVERY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63268,"idioma_id":4,"titulo":"Secochiliolide acid and some semi-synthetic derivatives affect the in vitro growth of Trypanosoma cruzi epimastigotes.","resumen":"Secochiliolide acid (1) isolated from the Patagonian shrub  Nardophyllum bryoides, was used as a scaffold for the preparation of a  series of nine derivatives. Compound 1 and its derivatives were tested  against Trypanosoma cruzi epimastigotes grown in liquid media. It was  first observed that secochiliolide acid (1) inhibited the proliferation  of the parasites, with an IC50 of 2 &#956;g\/ml. Six of the synthesized  derivatives were also active with IC50s between 2 and 7 &#956;g\/ml which are  comparable to that of the commercial drug benznidazole (2.5 &#956;g\/ml). These  results indicate that the carboxyl group is not essential for the  bioactivity of 1, while the presence of the tetrasubstituted exocyclic  double bond seems to be important. Moreover, the presence of the furan  and spirolactone rings is not essential for the bioactivity per se, but  is important in combination with other structural fragments present in  the molecule.","palabras_clave":"secochiliolide acid|Trypanosoma cruzi|Nardophyllum bryoides|trypanocidal activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63269,"idioma_id":4,"titulo":"XANES Study of the Radiation Damage on Alkanethiolates Capped Au Nanoparticles","resumen":"The  radiation  damage  during  XANES  experiments  on  alkanethiols  capped  gold nanoparticles has been investigated. Different carbon length chains and nanoparticle sizes have been studied. Changes in the spectra after 45 minutes of irradiation, using a bend magnet, were observed for chains with more than 6 carbon atoms and are associated with the cleavage of S-C bonds and formation of atomic sulphur on the nanoparticle surface.","palabras_clave":"RADIATION DAMAGE|XANES|ALKANETHIOLATES|AU NANOPARTICLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63270,"idioma_id":4,"titulo":"Antigenic and molecular studies of Parainfluenza virus type 3 isolated from cattle and buffalo in Argentina","resumen":"This work proposed the immunological and molecular study of antigenic variation that may exist between genotypes of Bovine Parainfluenza Virus type 3 (BPIV3). The results obtained in the cross IH assay demonstrated that sera against genotype A can inhibit both genotype heterologous C and homologous virus activity but the greater differences were observed when the sera against genotype B was assayed with genotype A virus. Phylogenetic analysis of the both buffaloes (genotyoe B) and cattle isolates (genotypes A and C) based on a fragment of the HN gene showed two well-defined clusters supported by a high bootstrap (73). In this study we report that the genotype B clustered with the genotype C and separated of the genotype A. The differences observed in the recognition of hyperimmune serum against genotype A when was assayed with the bubaline isolate of genotype B by Inhibition Haemagutination Assay (IHA) correlate with the observed differences in phylogenetic trees.","palabras_clave":"Water buffaloes| genotype B|Parainfluenza virus 3|Antigenic Differences"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63271,"idioma_id":4,"titulo":"Contribution of Genetic Background, Traditional Risk Factors, and HIV-Related Factors to Coronary Artery Disease Events in HIV-Positive Persons.","resumen":"Background&#8195;Persons infected with human immunodeficiency virus (HIV) have increased rates of coronary artery disease (CAD). The relative contribution of genetic background, HIV-related factors, antiretroviral medications, and traditional risk factors to CAD has not been fully evaluated in the setting of HIV infection. Methods&#8195;In the general population, 23 common single-nucleotide polymorphisms (SNPs) were shown to be associated with CAD through genome-wide association analysis. Using the Metabochip, we genotyped 1875 HIV-positive, white individuals enrolled in 24 HIV observational studies, including 571 participants with a first CAD event during the 9-year study period and 1304 controls matched on sex and cohort. Results&#8195;A genetic risk score built from 23 CAD-associated SNPs contributed significantly to CAD (P = 2.9×10(-4)). In the final multivariable model, participants with an unfavorable genetic background (top genetic score quartile) had a CAD odds ratio (OR) of 1.47 (95% confidence interval [CI], 1.05-2.04). This effect was similar to hypertension (OR = 1.36; 95% CI, 1.06-1.73), hypercholesterolemia (OR = 1.51; 95% CI, 1.16-1.96), diabetes (OR = 1.66; 95% CI, 1.10-2.49), &#8805;1 year lopinavir exposure (OR = 1.36; 95% CI, 1.06-1.73), and current abacavir treatment (OR = 1.56; 95% CI, 1.17-2.07). The effect of the genetic risk score was additive to the effect of nongenetic CAD risk factors, and did not change after adjustment for family history of CAD. Conclusions&#8195;In the setting of HIV infection, the effect of an unfavorable genetic background was similar to traditional CAD risk factors and certain adverse antiretroviral exposures. Genetic testing may provide prognostic information complementary to family history of CAD.","palabras_clave":"HIV |INFECTION|ANTIRETROVIRAL|THERAPY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63272,"idioma_id":4,"titulo":"Enhanced Photoacoustic Beam Profiling of Pulsed Lasers","resumen":"An improved version of a photoacoustic beam profiler of pulsed lasers is presented. The new model resorts to high-bandwidth condenser microphones to register higher-order, excited acoustic modes, thus enabling more accurate profiling. In addition, Xe was used as a buffer gas since its high atomic weight further reduces the eigenfrequencies. Furthermore, a new gas-handling system makes up for some deficiencies found in the first model. The system was calibrated using the Airy patterngenerated with a pinhole illuminated by a frequency-doubled Nd:YAG laser that excited NO2 traces. Once calibrated, the beam profile of aTEA CO2 laser was obtained, using ethylene as the absorbing species. This profiler returns more accurate profiles than thermal paper.","palabras_clave":"Fotoacústica|Haz láser"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63273,"idioma_id":4,"titulo":"Long-term Impact of No Tillage in Two Intensified Crop Rotations on Different Soil Organic Matter Fractions in Argentine Rolling Pampa","resumen":"It is expected that the agricultural intensification occurred in recent decades in the Argentine Rolling Pampa significantly  alters the SOM reserves. Therefore, it is necessary to identify soil organic carbon (C) and nitrogen (N) fractions to  understand the functionality and stabilization of these reserves. Our objectives were to study the NT effect in two crop  rotations, corn-double cropped wheat\/soybean (MWS) and double cropped wheat\/soybean (WS) on: 1) SOM and its  particle size and biological fractions contents, 2) C and N stubble biomass and 3) some soil properties in order to explain  the SOM differences found. The larger biomass residue remaining on the soil surface under NT promoted higher  aggregate stability and lower soil temperature and pH. At 0-5 cm soil depth, NT exhibited higher C and N contents, for  both uncomplexed and intimately associated to the mineral components fractions. However, the results indicated  variations in the SOM protection according to the rotation: in MWS the high aggregate stability showed better physical  protection, while in WS the greater cation exchange capacity and the lower value of N released by anaerobic incubation  would indicate the presence of transformed SOM. At 5-20 cm soil depth, only in WS, C microbial biomass was higher  with a low metabolic rate, indicating again the presence of highly decomposed SOM. The results obtained in WS under  NT would indicate the possibility of achieving slower recycled of the SOM.","palabras_clave":"NO TILLAGE|CROP ROTATIONS|SOIL ORGANIC CARBON FRACTIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63274,"idioma_id":4,"titulo":"Peritidal cyclic sedimentation from La Manga Formation (Oxfordian) Neuquén Basin, Mendoza, Argentina","resumen":"The La Manga Formation consists of marine carbonates and represents most of the sedimentary record ofthe Callovian-Oxfordian in the Neuquén Basin. Three localities in the southern Mendoza province werestudied and their cyclicity was determined by means of facies analysis and their vertical arrangement.Facies of inner ramp, that were deposited in extremely shallow-water environments with intermittentsubaerial exposures have been broken down into shallow subtidal, and intertidalesupratidal environments.Shallow subtidal facies are arranged into decimetre scale upward-shallowing cycles composed ofmarls, laminated or massive mudstones or bioclastic wackestones and intraclastic wackestoneepackstones.Intertidal-supratidal centimetre-scale cycles consist of an upward-shallowing succession ofrestricted facies, overlaid by horizontal or crinkle microbial laminites, flat pebble conglomerates orbreccias beds. The defined cycles show a shallowing upward trend in which the evidence of relativesea-level lowering is accepted. The interpretation of Fischer plots allowed the recognition of changes inaccommodation space.","palabras_clave":"estromatolitos|fosiles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63275,"idioma_id":4,"titulo":"Brucella abortus Invasion of Synoviocytes Inhibits Apoptosis and Induces Bone Resorption through RANKL Expression","resumen":"Arthritis is one of the most common complications of human active brucellosis, but its pathogenic mechanisms have not been completely  elucidated. In this paper, we describe the role of synoviocytes in the pathogenesis of brucellar arthritis. Our results indicate that  Brucella abortus infection inhibited synoviocyte apoptosis through the upregulation of antiapoptotic factors (cIAP-2, clusterin, livin,  and P21\/CIP\/CDNK1A). In contrast, infection did not change the expression of proteins that have been involved in apoptosis induction  such as Bad, Bax, cleaved procaspase 3, CytC, and TRAIL, among others; or their expression was reduced, as occurs in the case of  P-p53(S15). In addition, B. abortus infection induced upregulation of adhesion molecules (CD54 and CD106), and the adhesion of  monocytes and neutrophils to infected synoviocytes was significantly higher than to uninfected cells. Despite this increased adhesion,  B. abortus-infected synoviocytes were able to inhibit apoptosis induced by supernatants from B. abortus-infected monocytes and neutrophils.  Moreover, B. abortus infection increased soluble and membrane RANKL expression in synoviocytes that further induced  monocytes to undergo osteoclastogenesis. The results presented here shed light on how the interactions of B. abortus with synovial  fibroblasts may have an important role in the pathogenesis of brucellar arthritis","palabras_clave":"Brucella abortus|Synoviocytes|Apoptosis|Bone repsortion|RANKL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63276,"idioma_id":4,"titulo":"A Catalog of Aspect Refactorings for Spring\/AOP","resumen":"The importance of enterprise applications in current organizations makes it necessary to facilitate their maintenance and evolution along their life. These kind of systems are very complex and they have several requirements that orthogonally crosscut the system structure (called crosscutting concerns). Since many of the enterprise systems are developed with the Spring framework, can be taken advantage of the benefit provided by the aspect-oriented module of Spring in order to encapsulate the crosscutting concerns into aspects. In this way, the maintenance and evolution of the enterprise systems will be improved. However, most of the aspect refactorings presented in the literature are not directly applicable to Spring systems. Along this line, in this work we present an adaptation of a catalog of aspect refactorings, initially presented for AspectJ, to be used with Spring\/AOP. Also, we conduct a case study in which two enterprise applications developed with the Spring framework are refactored in order to encapsulate their crosscutting concerns into aspects.","palabras_clave":"aspect-oriented programming|refactoring|separation of concerns"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63277,"idioma_id":4,"titulo":"Toward automated refactoring of crosscutting concerns into aspects","resumen":"Aspect-oriented programing (AOP) improves the separation of concerns by encapsulating crosscutting concerns into aspects. Thus, aspect-oriented programing aims to better support the evolution of systems. Along this line, we have defined a process that assists the developer to refactor an object-oriented system into an aspect-oriented one. In this paper we propose the use of association rules and Markov models to improve the assistance in accomplishing some of the tasks of this process. Specifically, we use these techniques to help the developer in the task of encapsulating a fragment of aspectizable code into an aspect. This includes the choice of a fragment of aspectizable code to be encapsulated, the selection of a suitable aspect refactoring, and the analysis and application of additional restructurings when neces- sary. Our case study of the refactoring of a J2EE system shows that the use of the process reduces the intervention of the developer during the refactoring.","palabras_clave":"Software evolution|Separation of concerns|Aspect refactoring"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63278,"idioma_id":4,"titulo":"Influence of MDR1 C1236T polymorphism on lopinavir plasma concentration and virological response in HIV-1-infected children","resumen":"Background: Variability in MDR1 and PXR has been associated with differences in drug plasma levels and response to antiretroviral therapy. We investigated whether polymorphisms in MDR1 (T-129C, C1236T and C3435T) and PXR (C63396T) affect lopinavir plasma concentration and the virological or immunological response to HAART in HIV-1-infected children. Methods: Genotypes were identified in 100 blood donors and 38 HIV-1-infected children. All children received HAART with lopinavir boosted with ritonavir (LPV\/r) at the time of LPV plasma level quantification, before (Ctrough) and between 1 and 2 h after (Cpost-dose) the administration of the next dose of drug. CD4+ T-cell counts and plasma viral load were analyzed before and after the initiation of LPV\/r. Results: MDR1 1236T, MDR1 3435T and PXR 63396T alleles showed a frequency of ~50% while the MDR1 -129C allele only reached 5%. Children heterozygotes 1236CT showed a significantly lower LPV Cpost-dose than homozygotes 1236TT (median Cpost-dose = 3.04 &#956;g\/ml and 6.50 &#956;g\/ml, respectively; p = 0.016). Children heterozygotes 1236CT also had a lower decrease of viral load after 36 weeks of LPV\/r exposure compared with homozygotes1236CC (median viral load changes = &#8722;0.50 log10 copies\/ml and &#8722;2.08 log10 copies\/ml, respectively; p = 0.047). No effect on the immunological response was observed for polymorphisms of MDR1 or PXR.Conclusions: Our results suggest that the MDR1 C1236T SNP significantly reduces LPV plasma concentration affecting the virological response to HAART. Heterozygotes 1236CT might have an altered level of P-gp expression\/activity in enterocytes and CD4+ T lymphocytes that limits the absorption of LPV leading to an impaired virological suppression.","palabras_clave":"MDR-1|HIV-1|Pediatría|Lopinavir"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63279,"idioma_id":4,"titulo":"Short-term analysis of the phytoplankton structure and dynamics in two ponds with distinct trophic states from Cierva Point (maritime Antarctica)","resumen":"Abstract  Phytoplankton communities dominating Musgos and Papúa ponds with differing trophic states were sampled over 3 days enabling the detection of the physiological and population responses of microalgae to short-scale changes in biotic and abiotic factors, rather than frequently analyzed changes in community composition responses to long-scale environmental changes. We hypothesized that both environments undergoing diel changes would be dominated by phytoplankton with generalist strategies, while community structure would be mostly dictated by the trophic state of each water body. The phytoplankton biovolumes of both ponds were strongly dominated by euplanktonic nanoflagellated Chlorophyta, while phycocyanin-rich picocyanobacteria dominated the picophytoplankton. Parallel diel cycles of air and water temperatures were more pronounced on a sunny, warm day which prompted algal photosynthesis, revealed by strong increases in dissolved oxygen and pH. Nutrient and phytoplanktonic chlorophyll a confirmed the hypertrophic condition of Papúa pond. This accounted for the distinct community composition encountered in each pond, which remained stable throughout the study, as revealed by the SIMI index. The inverse relationship between the chl a\/abundance ratio and the abundances of dominant species together with varying net growth rates (k&#8242;) showed algal reproduction, yet densities remained rather stable in both cases. In Musgos pond, fluctuations in k&#8242; for small and median ciliates shadowed those of pico- and nanophytoplankton, respectively, strongly suggesting that they can control algal growth in these 2-level trophic chains.","palabras_clave":"maritime Antarctica|ponds|phytoplankton structure|short-term study"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63280,"idioma_id":4,"titulo":"Polysaccharide-synthesizing Glycosyltransferases and Carbohydrate Binding Modules: the case of Starch Synthase III","resumen":"Glycosyltransferases (GTs) are a ubiquitous group of enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Nucleotide-sugars, lipid phosphate sugars and phosphate sugars can act as activated donor molecules while acceptor substrates involve carbohydrates, proteins, lipids, DNA and also, numerous small molecules (i. e. antibiotics, flavonols, steroids). GTs enzyme families are very ancient. They are founded in all the three domains of life and display three different folds (named GT-A, GTB and GT-C) which are a variant of a common &#945;\/&#946; scaffold. In addition, several GTs contain an associated non-catalytic carbohydrate binding module (CBM) that could be critical for enzyme activity. This work reviews the current knowledge on the GTs structures and functions and highlights those enzymes that contain CBMs, particularly starch binding domains (SBDs). In addition, we also focus on A. thaliana starch synthase III enzyme, from the GT-5 family. This protein has a GT-B fold, and contains in its N-terminal region three in tandem SBDs, which are essential for the regulation of enzyme activity.","palabras_clave":"carbohydrate binding modules|enzymes|glysosyltransferases|starch"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63281,"idioma_id":4,"titulo":"Landscape determinants of Saint Louis encephalitis human infections in Córdoba city, Argentina during 2010","resumen":"AbstractBackgroundSaint Louis Encephalitis virus (SLEV) is endemic in Argentina. During 2005 an outbreak occurred in Cordoba, being the first register of epidemic activity in South America. In the following years only sporadic cases were registered, but during summer-autumn of 2010 an outbreak occurred in Cordoba city with a lower magnitude than the one reported in 2005. Understanding the association of different landscape elements related to SLEV hosts and vectors in urban environments is important for identifying high risk areas for human infections, which was here evaluated. MethodsThe current study uses a case-control approach at a household geographical location, considering symptomatic and asymptomatic human infections produced by SLEV during summer-autumn 2010 in Cordoba city. Geographical information systems and logistic regression analysis were used to study the distribution of infected human cases and their proximity to water bodies, vegetation abundance with a threshold value of Normalized Difference Vegetation Index (NDVI) higher than 0.3, agricultural fields and housing density classified as high\/low density urban constructions. Population density at a neighborhood level was also analyzed as a demographic variable.  Results Logistic regression analysis revealed vegetation abundance (NDVI>0.3) was significantly and positively associated with the presence of human infections by SLEV. Areas with lower density of urban constructions were also important to predict the occurrence of SLEV infections. Other variables like different types of water bodies and agricultural fields were not associated to the occurrence of human infections by this virus. The logistic model was used to develop a map of probability of human infections in Cordoba city. The population density analysis shows that SLEV infections are more likely to occur when population density by neighborhood is lower.   ConclusionsThe model highlights areas that are most likely to experience SLEV infections. Major landscape contributing to the outbreak of SLEV in 2010 were the proximity to places with NDVI>0.3 (parks, squares, riversides) and the presence of low density urban constructions, like residential areas. These findings and the predictive map developed could be important and useful for public health surveillance and to improve prevention of vector-borne diseases.","palabras_clave":"ARBOVIRUS|ENCEPHALITIS|OUTBREAK|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63282,"idioma_id":4,"titulo":"Spatial Patterns of High Aedes aegypti Oviposition Activity in Northwestern Argentina","resumen":"Background: In Argentina, dengue has affected mainly the Northern provinces, including Salta. The objective of this study was to analyze the spatial patterns of high Aedes  aegypti oviposition activity in San Ramón de la Nueva Orán, northwestern Argentina. The location of clusters as hot spot areas should help control programs to identify priority areas and allocate their resources more effectively.Methodology: Oviposition activity was detected in Orán City (Salta province) using ovitraps, weekly replaced (October 2005- 2007). Spatial autocorrelation was measured with Moran´s Index and depicted through cluster maps to identify hot spots. Total egg numbers were spatially interpolated and a classified map with Ae. aegypti high oviposition activity areas was performed. Potential breeding and resting (PBR) sites were geo-referenced. A logistic regression analysis of interpolated egg numbers and PBR location was performed to generate a predictive mapping of mosquito oviposition activity.Principal Findings: Both cluster maps and predictive map were consistent, identifying in central and southern areas of the city high Ae. aegypti oviposition activity. A logistic regression model was successfully developed to predict Ae. aegypti oviposition activity based on distance to PBR sites, with tire dumps having the strongest association with mosquito oviposition activity. A predictive map reflecting probability of oviposition activity was produced. The predictive map delimitated an area of maximum probability of Ae. aegypti oviposition activity in the south of Orán city where tire dumps predominate. The overall fit of the model was acceptable (ROC = 0.77), obtaining 99% of sensitivity and 75.29% of specificity. Conclusions: Distance to tire dumps is inversely associated with high mosquito activity, allowing us to identify hot spots. These methodologies are useful for prevention, surveillance, and control of tropical vector borne diseases and might assist National Health Ministry to focus resources more effectively.","palabras_clave":"SPATIAL|DISTRIBUTION|OVIPOSITION|DENGUE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63283,"idioma_id":4,"titulo":"An integrate analysis of an Hauterivian coral biostrome from the Agrio Formation, Neuquén Basin, west-central Argentina","resumen":"Abstract: A coral biostrome from the lower Hauterivian of the Neuquén Basin, west-central Argentina, was examined in detail. Taxonomic analysis established it as a monospecific assemblage of the scleractinian colonial coral Stereocaenia triboleti (Koby). Palaeoecological analysis of the species allows us to infer that it was an opportunistic species, capable of thriving in mixed carbonatesiliciclatic environments, on a soft substrate under a moderate to low sedimentation rate showing great regenerative capacity. Taphonomic analysis, together with lithofacies and microfacies analysis, allowed us to establish that the coral biostrome was deposited in a middle ramp setting under open marine and well-oxygenated conditions. Corals colonized a soft substrate during a transgressive phase and reaching its climax in the maximum flooding zone under lowest clastic input. They managed to form a low coral meadow of ramose forms of up to 30 cm in height. Coral remains were exposed on theinterface after death and thus were subject to severe encrustation and bioerosion. The coral biostrome is interpreted as a mixed biogenic-sedimentologic skeletal concentration deposited by an interplay of an initial biogenic concentration through later episodes of physical reworking, probably storms, which yielded a largely parautochthonous fossil assemblage with minimum lateral transport. As sedimentation rate increased during a highstand interval, the coral-dominated community could not cope with it and was finally buried. The described coral biostrome recorded at the base of a shallowing-upwards cycle closely resembles coral biostromes described from the Hauterivian of Peru and Turkmenistan and differs significantly from framework reef facies recorded in the Hauterivian of the Tethys region from the Paris Basin to Crimea.","palabras_clave":"SCLERACTINIA|STEREOCAENIA|AGRIO FORMATION|HAUTERIVIAN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63284,"idioma_id":4,"titulo":"Serpulids and other calcareous tube-dwelling encrusting polychaetes from the Early Cretaceous  Agrio Formation (Neuquén Basin, Argentina)","resumen":"                    Serpulids  and other related tube-dwelling polychaetes are often ignored when found as  fossil remains. They are, however, a widespread and important group today, and  abundant literature has been published on them. Knowledge of fossil serpulids  is centered on European specimens, and little has been done on South American  fossil calcareous tubes. In this paper, seven serpulid and sabellid morphotypes  are described from the Early Cretaceous marine Agrio Formation of Argentina,  revealing a diversity of worms recorded as encrusters on bivalves, ammonites  and corals. Sabellids are represented by Glomerula  cf. serpentina. Serpulids are  represented by two subfamilies: 'Serpulinae' includes Mucroserpula&nbsp; mucroserpula, Parsimonia  antiquata, Placostegus cf. conchophilus, Propomatoceros&nbsp; semicostatus  and P. sulcicarinatus; Spirorbinae is  represented by heavily worn tiny coiled tubes assigned to Neomicrorbis?. Serpulids and sabellids are one of the main  components of the mollusk encrusting fauna recorded in the Agrio Formation,  along with small oysters but much more diverse. They are most commonly found as  post-mortem encrusters, but some cases of unquestionable living interaction  were also found, remarkably serpulid tubes embedded on coral branches. They are  often overgrown by bryozoans, and sometimes by oysters, and frequently occur  aggregated.","palabras_clave":"SERPULID|SABELLID|EARLY CRETACEOUS|ENCRUSTER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63285,"idioma_id":5,"titulo":"Prevalencia De Consumo De Tabaco Y Variables Psicosociales En Profesionales Psicólogos De Argentina","resumen":"Uno de los factores que influencia el consumo de tabaco en la población general es el consumo en los profesionales de la salud. Ese consumo se ha estudiado fundamentalmente en médicos. Se determinó la prevalencia de consumo de tabaco y otras variables psicosociales en profesionales psicólogos empleando un diseño observacional transversal. Se indagó: consumo de tabaco, estadios de cambio,tentación, nivel de adicción, aceptabilidad social. El cuestionario fue administrado a una muestra intencional de 1204 psicólogos. Los resultados arrojan una prevalencia del 34.1%, un 57.5% de la muestra se encuentra en el estadio de precontemplación. La prevalencia de consumo de tabaco en psicólogos resulta mayor que en población general y existe una alta resistencia para dejar de fumar.","palabras_clave":"CONSUMO DE TABACO|PSICOLOGOS|ESTADIOS DE CAMBIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63286,"idioma_id":4,"titulo":"The Farallon Aluk ridge collision with South America: Implications for the geochemical changes of slab window magmas from fore- to back-arc","resumen":"The collision of a divergent ocean ridge may evolve into two end cases: in the continuity of ocean-floor subduction, or in the detachment of the subducted plate. The northern Patagonia active plate margin has the unique situation that in Cenozoic time it has been subjected to two divergent ridge collisions, each one representing one of the end members. The Neogene Antarctica-Nazca divergent ridge collision evolved as a continuous ocean-floor subduction system, promoting a magmatic hiatus at the arc axis, the obduction of part of the ridge ocean-floor in the fore-arc, and basaltic volcanism in the back-arc. In contrast, the Paleogene Farallon-Aluk divergent ridge collision evolved into a transform margin, with the detachment and sinking of the Aluk plate and the development of a large slab window. As in the previous case, this collision promoted a magmatic hiatus at the arc axis, but the tectono-magmatic scenario changed to postorogenic synextensional volcanism that spread to the former fore-arc (basalt, andesite, rhyolite) and former back-arc (bimodal ignimbrite flare-up, basalt). Geochemistry of this slab window synextensional volcanism shows more MORB-like basalts towards the former fore-arc, and MORB-OIB-like basalts towards the former back-arc. Instead, an isolated undeformable crustal block in the former back-arc, with an ?epeirogenic? response to the slab window and extensional regime, was covered by OIB-type basalts after uplift. Major elements show that slab window basalts reach TiO2 values up to 3 wt%, as compared with the top value of 1.5 wt% of arc magmas. Besides, the MgO with respect to (FeOt + Al2O3) ratio helps to distinguish slab window magma changes from the former fore-arc to the former back-arc and also with respect to the ?epeirogenic? block. Higher contents of HFS elements such as Nb and Ta also help to distinguish this slab window from arc magmas and also, to distinguish slab window magma changes from the former fore-arc to the former back-arc and ?epeirogenic? block settings. The isotope compositions of slab window magmatism show a disparate coeval array from MORB to crustal sources, interpreted as a consequence of the lack of protracted storage and homogenization due to the extensional setting.","palabras_clave":"ALUK PLATE|PETROLOGY|SLAB WINDOW|MORB"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63287,"idioma_id":4,"titulo":"Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity","resumen":"As part of our ongoing research on the antibacterial activity of Achyrocline satureioides, this study seeks to  better understand the interactions between the metabolites isolated from this plant. For this purpose, the  combined effect of 23-methyl-6-O-desmethylauricepyrone (1), quercetin (2) and 3-O-methylquercetin  (3), obtained through bioguided fractionation from A. satureioides ethanol extract, was evaluated against  Staphylococcus aureus and Escherichia coli. In first place, the antibacterial effect of the combination of  flavonols 2 and 3 was assessed, as these showed individual effectiveness lower than or equal to that of  the fraction from which they were obtained. When the flavonols were applied together at concentrations  below their minimum inhibitory concentration (MIC) values, a synergistic effect (FICI < 0.30) against S.  aureus was observed. In addition, compounds 2 and 3 in combination reduced 1000 times the MIC of  compound 1, showing a clear synergistic interaction (FICI < 0.15) in treatments against the Gram (+) bacterium.  The most active combination against E. coli showed an additive interaction (FICI < 0.62) between  the three assayed compounds 1\u20133. These results indicated the existence of concerted action between  these metabolites, evidence of the importance of the synergistic interactions between the components  of plant-derived extracts for the control of pathogenic bacteria","palabras_clave":"ACHYROCLINE SATUREIOIDES|SYNERGISM|NATURAL ANTIBIOTICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63288,"idioma_id":4,"titulo":"Analysis of the structure, configuration and sizing of Cu and Cu oxide nanoparticles generated by fs laser ablation of solid target in liquids","resumen":"We report on the analysis of structure, configuration, and sizing of Cu and Cu oxide nanoparticles (Nps) produced by femtosecond (fs) laser ablation of solid copper target in liquids. Laser pulse energy ranged between 500 &mu;J and 50 &mu;J. Water and acetone were used to produce the colloidal suspensions. The study was performed through optical extinction spectroscopy using Mie theory to fit the full experimental spectra, considering free and bound electrons size dependent contributions to the metal dielectric function. Raman spectroscopy and AFM technique were also used to characterize the sample. Considering the possible oxidation of copper during the fabrication process, two species (Cu and Cu2O) arranged in two structures (bare core or core-shell) and in two configuration types (Cu-Cu2O or Cu2O-Cu) were considered for the fitting depending on the laser pulse energy and the surrounding media. For water at high energy, it can be observed that a Cu-Cu2O configuration fits the experimental spectra of the colloidal suspension, while for decreasing energy and below a certain threshold, a Cu 2O-Cu configuration needs to be included for the optimum fit. Both species coexist for energies below 170 &mu;J for water. On the other hand, for acetone at high energy, optimum fit of the full spectrum suggests the presence a bimodal Cu-Cu2O core-shell Nps distribution while for decreasing energy and below a 70 &mu;J threshold energy value, Cu2O-Cu core-shell Nps must be included, together with the former configuration, for the fit of the full spectrum. We discuss possible reasons for the changes in the structural configuration of the core-shell Nps. &copy; 2013 American Institute of Physics.","palabras_clave":"NANOPARTICLES|COPPER AND COPPER OXIDE|LASER ABLATION|FEMTOSECOND"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63289,"idioma_id":4,"titulo":"Neuromodulatory role of Angiotensin-(1-7) in the central nervous system","resumen":"Angiotensin (Ang) (1-7) constitutes an important functional end-product of the renin-angiotensin system endogenously formed from Ang I or Ang II through the catalytic activity of angiotensin-converting enzyme (ACE) 2, prolyl-carboxypeptidase, neutral endopeptidase or other endopeptidases. Ang-(1-7) lacks the pressor, dipsogenic or stimulatory effect on aldosterone release characteristic of Ang II. In contrast, it produces vasodilation, natriuresis and diuresis and inhibits angiogenesis and cell growth. At the central level, Ang-(1-7) acts in sites involved in the control of cardiovascular function, thus contributing to blood pressure regulation. This action may result from its inhibitory neuromodulatory action on norepinephrine (NE) levels at the synaptic cleft. I.e., Ang-(1-7) reduces NE release and synthesis, while it causes an increase in the NE transporter expression, contributing in this way to centrally NE neuromodulation. Thus, by selective neurotransmitter release Ang-(1-7) may contribute to the overall central cardiovascular effects. Here, we summarize the central effects of Ang-(1-7) and the mechanism by which the peptide modulates NE levels in the synaptic cleft. We also provide new evidences of its cerebroprotective role.","palabras_clave":"Angiotensin-(1-7)|Mas Receptor|Norepinephrine|Tyrosine hydroxylase"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63290,"idioma_id":4,"titulo":"Metacaspases, autophagins and metallocarboxypeptidases:  Potential new targets for chemotherapy of the Trypanosomiases.","resumen":"During the last decade, de novo drug discovery approaches have come into focusdue to the increased number of parasite pathogen genomes sequenced and thesubsequent availability of genome-scale functional datasets. In order toprioritize target proteins, these approaches consider traits commonly thought to be desirable in a drug target, including essentiality, druggability (whetherdrug-like molecules are likely to interact with the target), assayability,importance in lifecycle stages of the pathogen relevant to human health, andspecificity (i.e. the target is absent from, or substantially different in, thehost). Proteases from protozoan parasites have become popular drug targets since these enzymes accomplish both housekeeping tasks common to many eukaryotes aswell as functions highly specific to the parasite life style. Trypanosoma cruzi, the parasitic flagellate, agent of Chagas Disease, contains several cysteine,serine, threonine and metallo proteinases. This review will deal with peculiarfamilies described in this parasite. Among them, two eukaryote homologues of the carboxypeptidases Taq are promising targets due to their particular phylogenetic distribution. Also absent in metazoans, metacaspases are essential peptidasesplaying important roles in cell growth, death and differentiation oftrypanosomatids. Finally, autophagins are involved in the regulation of aconserved degradative pathway, the autophagy pathway, and result important forparasite survival under nutritional stress conditions and differentiation.Although so far there are no specific inhibitors for these families, theincreasing knowledge of their biochemical properties, including substratespecificity, crystal structure, and biological functions, is an essential steptowards the development of inhibitors.","palabras_clave":"Trypanosomatids|Metacaspases|Autophagins|Metallocarboxypeptidases"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63291,"idioma_id":4,"titulo":"A Supramolecular Assembly Formed by Heteroassociation of Ciprofloxacin and Norfloxacin in the Solid State: Co-Crystal Synthesis and Characterization","resumen":"The interaction in the solid state between ciprofloxacin (CIP) and norfloxacin (NOR) was investigated and a new multicomponent molecular complex (COP) was obtained and characterized. It is composed of equimolar quantities of both compounds, according to TLC and 1H NMR data. As single-crystal X-ray analysis showed, COP crystallizes in the triclinic system, space group P(1&#773;). The asymmetric unit comprises three independent zwitterions (A, B, and C) with contributions of both CIP and NOR, as well as 14.6 water molecules. At the zwitterion A site, the occurrence is about 37% (CIP), 63% (NOR); at the B site, there is predominantly CIP (70%); and at the C site the occurrence is about 43% (CIP) and 57% (NOR), yielding an overall 1:1 CIP-NOR ratio in the crystal. TG data evidenced a one-step loss of solvent (about 18% per COP zwitterion) below 110 °C, confirming the presence of water molecules. The propensity for heteroassociation between CIP and NOR, under the conditions described herein, originated a co-crystal reported for the first time between FQs, and became one of the most interesting aspects of this research.","palabras_clave":"Cocristal|Norfloxacin|Ciprofloxacin|Heteroassociation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63292,"idioma_id":4,"titulo":"Changing patterns of marine resource exploitation by hunter-gatherers throughout the late Holocene of Argentina are uncorrelated to sea surface temperature","resumen":"During the late Holocene, the coastal marine resources of southern South America were exploited by both marine hunter-gatherers with aquatic mobility in the Beagle Channel and the Chilean archipelago and terrestrial hunter-gatherers who inhabited various coastal settings in Argentina. Although the two cultures differed in technology and in historical exploitation patterns, they both used otariids, molluscs,fishes and sea birds as sources of food and raw materials for centuries. Ultimately, their use of these resources, particularly of otariids, declined strongly at different times. Overexploitation has been suggested as the main reason for this pattern, at least in the Beagle Channel, but similar declines in the north Pacific have been attributed to an increase in sea surface temperature (SST). The present paper tests the latter hypothesis in southern South America by using the d18O of bivalve shells (Aulacomya atra atra and Mytilus edulis) collected at archaeological sites as a proxy for SST and comparing the patterns of d18O with the patterns of resource exploitation by hunter-gatherers. Samples were collected from the Beagle Channel and the central-northern Patagonian coast (north to 43 S) to generate two comparable datasets.The results suggest that SST increased in both areas at the beginning of the late Holocene and was slightly higher than at present during most of that period, except during the Little Ice Age, when values were similar to those recorded at the end of the middle Holocene were found. The relative importance of otariids, mainly Arctocephalus australis, in the economy of the inhabitants of the Beagle Channel declined as SST increased, but otariid exploitation did not intensify again during the Little Ice Age. On the contrary, the intensity of otariid exploitation in central-northern Patagonia, mainly Otaria flavescens, was unrelated to the changes in d18O. Thus, changes in SST are unlikely to be the major driver of these resource exploitation patterns.","palabras_clave":"Sea surface temperature|Stable isotopes|Patagonia|Hunter-gatherers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63293,"idioma_id":4,"titulo":"Retrieving the relative contribution of aerosol types from single particle analysis and radiation measurements and calculations: a comparison of two independent approaches","resumen":"The main purpose of this work is to determine the relative contribution of different types of aerosols at an urban site by using two independent approaches: individual particle analysis and radiative transfer calculations and irradiance measurements. To accomplish that purpose, we used our UV-B irradiance (280-315 nm) dataset, the AERONET (AErosol RObotic NETwork) database, the SEM (Scanning Electron Microscopy, LEO 1450VP) analyses of the collected particles and the Santa Barbara DISORT Atmospheric Radiative Transfer (SBDART) model. On one hand, the collected particles were analyzed by SEM-EDX (Energy Dispersive X-Ray, Genesis 2000) in order to determine their chemical composition. Then, by using a developed algorithm they were classified as rural or urban, resulting in a (24±3)% of rural and (76±8)% of urban. On the other hand, aerosols were incorporated into the SBDART model through two of its default profiles (urban or rural) and by using the Aerosol Optical Depth (AOD) provided by AERONET. The aerosol effect on experimental surface UV-B irradiance was reproduced by a linear combination of the irradiances calculated by using these profiles. From this analysis we found that, in average, a mix of aerosols of (30±3)% rural and (70±7)% urban explains the observed reduction in the experimental irradiance. Considering the agreement between the results obtained by using these two independent methodologies, the use of the irradiance as a surrogate variable to retrieve aerosol types is discussed. The methodology presented here is applicable to any site provided irradiance measurements and AOD are available.","palabras_clave":"AEROSOL TYPES|SINGLE PARTICLE ANALYSIS|SEM-EDX|SBDART model|UV-B IRRADIANCE|AEROSOL OPTICAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63294,"idioma_id":4,"titulo":"Indigenous filamentous fungi on the surface of Argentinean dry fermented sausages produced in Colonia Caroya (Córdoba)","resumen":"Some producers of dry fermented sausages use fungal starter cultures with the aim to achieve a desirable surface appearance and avoid the growth of mycotoxigenic fungi. These commercial cultures are mainly composed of Penicillium nalgiovense biotype 6. In contrast, in the case of producers who do not use starters, sausages are spontaneously colonized by the house mycobiota, which generally consists of heterogeneous molds corresponding to different genera and species. In this work, the surface mycobiota of dry fermented sausages produced in Colonia Caroya (Córdoba, Argentina) was determined in both summer and winter seasons. All the sausages sampled had been made without the use of surface fungal starters. &nbsp;In the 57 sausages analyzed in the two winter seasons studied (2010 and 2012), we found a total of 95 isolates of filamentous fungi belonging to six genera (Penicillium, Aspergillus, Mucor, Cladosporium, Scopulariopsis and Eurotium) and ten fungal species, whereas in the 36 sausages analyzed in the two summer seasons studied (2011 and 2012), we found 89 isolates belonging to five genera (Penicillium, Aspergillus, Mucor, Cladosporium and Geotrichum) and ten fungal species. Although 16 different species were found in both winter and summer seasons, only 2 of them predominated completely. P. nalgiovense was found in almost 100% of the sausages analyzed, where biotype 4 was the most frequent. This species gives a whitish gray coloration to the sausages. Considering that the factories sampled do not use fungal starter cultures, this predominance is very interesting since mycotoxin production by this fungus has not been reported. A. ochraceus was isolated with a frequency of 80-90% in the summer seasons, but in none of the winter samples. The presence of this fungus in sausages produced in the summer was attributed to the high environmental temperatures and the uncontrolled temperature in the ripening rooms during the night. In all cases, A. ochraceus was responsible for the undesirable yellowish gold color of the casing. This fungus thus causes significant economic losses to the producers of Colonia Caroya during the months of high temperatures.           ","palabras_clave":"FILAMENTOUS FUNGI|SAUSAGES|PENICILLIUM NALGIOVENSE|ASPERGILLUS OCHRACEUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63295,"idioma_id":4,"titulo":"'Microstructure and magnetic properties of as-cast Ni2MnGa alloys processed by twin roller melt spinning","resumen":"The magnetismandthemicrostructureofNi2MnGaalloys,processedforthefirsttimebytwinroller  melt spinningattangentialwheelspeedsof10m\/s(V10),15m\/s(V15)and20m\/s(V20)are  investigated.Atroomtemperature,themajorphaseintheas-castalloysisthecubicL21 Ni2MnGa  ordered austeniticphase,withalatticeparameteronly 0.1% largerthanthetabulatedvalue.The  order domainsizeintheausteniticphasedecreasesfrom(4071) nminsamplesV10to(1971) nmin  V20.Mn(S,Se)smallprecipitatesarealsofounduniformlyembeddedintheribbonswithmeansizeof  (2672) nm(V10)and(772) nm(V20),exhibitingadefiniteorientationrelationwiththeaustenitic  matrix, o1 0 04P\/\/ o1 0 04a. Theas-castalloystransformtoanintermediatecubicphaseIatabout  220?230Kdependingonthequenchingrateandtoamartensiticphaseatabout130K.Thehigh  temperatureausteniticphaseandthelowtemperaturemartensiticphaseareferromagnetic;inboth  cases thesaturationpolarizationislowerinsamplesquenchedathigherrates.Thedemagnetization  curvesmeasuredfromsaturationinthemartensiticstateshowtwomarkedsteps:afirstonefor  positivefieldsinV15andV10(46 mT)andalargersecondoneforrelativelylargeinversefields  (130?250 mT)inallthesamples.Thesestepsarelikelytoarisefromademagnetizationmechanism  involvingafieldinducedtwinboundarymotioninthefewmartensitevariantsselectedbythe  crystallographictextureandthequenchedstressesintheribbons","palabras_clave":"Ni2MnGa alloy |Twin rolle rmeltspinning |Shape memory |X-ray diffraction| TEM |Magnetic property"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63296,"idioma_id":4,"titulo":"Multicellular spheroid formation and evolutionary conserved behaviors of apple snail hemocytes in culture","resumen":"A hemocyte primary culture system for Pomacea canaliculata in a medium mimicking hemolymphatic plasma composition was developed. Hemocytes adhered and spread onto culture dish in the first few hours after seeding but later began forming aggregates. Time-lapse video microscopy showed the dynamics of the early aggregation, with cells both entering and leaving the aggregates. During this period phagocytosis occurs and was quantified. Later (>4 h), hemocytes formed large spheroidal aggregates that increased in size and also merged with adjacent spheroids (24e96 h). Large single spheroids and spheroid aggregates detach from the bottom surface and float freely in the medium. Correlative confocal,  transmission electron and phase contrast microscopy showed a peculiar organization of the spheroids,with a compact core, an intermediate zone with large extracellular lacunae and an outer zone of flattened cells; also, numerous round cells emitting cytoplasmic extensions were seen attaching to the spheroids\u2019smooth surface. Dual DAPI\/propidium iodide staining revealed the coexistence of viable and non-viable cells within aggregates, in varying proportions. DNA concentration increased during the first 24 h of culture and stabilized afterward. BrdU incorporation also indicated proliferation. Spontaneous spheroid formation in culture bears interesting parallels with spheroidal hemocyte aggregates found in vivo in P. canaliculata, and also with spheroids formed by tumoral or non-tumoral mammalian cells in vitro.","palabras_clave":"APPLE SNAILS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63297,"idioma_id":4,"titulo":"Barrier Properties and Structural Study of Nanocomposite of HDPE\/Montmorillonite Modified with Polyvinylalcohol","resumen":"In this work was studied the permeation of CO2 in films of high-density polyethylene (HDPE) and organoclay modified withpolyvinylalcohol (MMTHDTMA\/PVA) obtained frommelt blending. Permeation study showed that the incorporation of the modifiedorganoclay generates a significant effect on the barrier properties of HDPE. When a load of 2wt% of MMTHDTMA\/PVA wasincorporated in the polymer matrix, the flow of CO2 decreased 43.7% compared to pure polyethylene.The results of TEM showedthat clay layers were dispersed in the polymeric matrix, obtaining an exfoliated-structure nanocomposite. The thermal stabilityof nanocomposite was significantly enhanced with respect to the pristine HDPE. DSC results showed that the crystallinity wasmaintained as the pure polymericmatrix. Consequently, the decrease of permeability was attributable only to the effect of tortuositygenerated by the dispersion ofMMTHDTMA\/PVA. Notably themechanical properties remain equal to those of pure polyethylene, butwith an increase in barrier properties to CO2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.2 in films of high-density polyethylene (HDPE) and organoclay modified withpolyvinylalcohol (MMTHDTMA\/PVA) obtained frommelt blending. Permeation study showed that the incorporation of the modifiedorganoclay generates a significant effect on the barrier properties of HDPE. When a load of 2wt% of MMTHDTMA\/PVA wasincorporated in the polymer matrix, the flow of CO2 decreased 43.7% compared to pure polyethylene.The results of TEM showedthat clay layers were dispersed in the polymeric matrix, obtaining an exfoliated-structure nanocomposite. The thermal stabilityof nanocomposite was significantly enhanced with respect to the pristine HDPE. DSC results showed that the crystallinity wasmaintained as the pure polymericmatrix. Consequently, the decrease of permeability was attributable only to the effect of tortuositygenerated by the dispersion ofMMTHDTMA\/PVA. Notably themechanical properties remain equal to those of pure polyethylene, butwith an increase in barrier properties to CO2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.HDTMA\/PVA) obtained frommelt blending. Permeation study showed that the incorporation of the modifiedorganoclay generates a significant effect on the barrier properties of HDPE. When a load of 2wt% of MMTHDTMA\/PVA wasincorporated in the polymer matrix, the flow of CO2 decreased 43.7% compared to pure polyethylene.The results of TEM showedthat clay layers were dispersed in the polymeric matrix, obtaining an exfoliated-structure nanocomposite. The thermal stabilityof nanocomposite was significantly enhanced with respect to the pristine HDPE. DSC results showed that the crystallinity wasmaintained as the pure polymericmatrix. Consequently, the decrease of permeability was attributable only to the effect of tortuositygenerated by the dispersion ofMMTHDTMA\/PVA. Notably themechanical properties remain equal to those of pure polyethylene, butwith an increase in barrier properties to CO2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.HDTMA\/PVA wasincorporated in the polymer matrix, the flow of CO2 decreased 43.7% compared to pure polyethylene.The results of TEM showedthat clay layers were dispersed in the polymeric matrix, obtaining an exfoliated-structure nanocomposite. The thermal stabilityof nanocomposite was significantly enhanced with respect to the pristine HDPE. DSC results showed that the crystallinity wasmaintained as the pure polymericmatrix. Consequently, the decrease of permeability was attributable only to the effect of tortuositygenerated by the dispersion ofMMTHDTMA\/PVA. Notably themechanical properties remain equal to those of pure polyethylene, butwith an increase in barrier properties to CO2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.2 decreased 43.7% compared to pure polyethylene.The results of TEM showedthat clay layers were dispersed in the polymeric matrix, obtaining an exfoliated-structure nanocomposite. The thermal stabilityof nanocomposite was significantly enhanced with respect to the pristine HDPE. DSC results showed that the crystallinity wasmaintained as the pure polymericmatrix. Consequently, the decrease of permeability was attributable only to the effect of tortuositygenerated by the dispersion ofMMTHDTMA\/PVA. Notably themechanical properties remain equal to those of pure polyethylene, butwith an increase in barrier properties to CO2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.HDTMA\/PVA. Notably themechanical properties remain equal to those of pure polyethylene, butwith an increase in barrier properties to CO2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.2. This procedure allows obtaining nanocomposites of HDPE with a good barrierproperty to CO2 which would make it competitive in the use of packaging.2 which would make it competitive in the use of packaging.","palabras_clave":"PERMEATION CO2|HDPE|ORGANOCLAY|PVA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63298,"idioma_id":4,"titulo":"POD-spectral decomposition for fluid flow analysis and model reduction","resumen":"We propose an algorithm that combines proper orthogonal decomposition with a spectral method to analyze and extract reduced ordermodels of flows from time data series of velocity fields. The flows considered  in this study are assumed to be driven by non-linear dynamical systems exhibiting a complex behavior within quasiperiodic orbits in the phase space. The technique is appropriate to achieve efficient reduced order models even in complex cases for which the flow description requires a discretization with a fine spatial and temporal resolution. The proposed analysis enables to decompose complex flow dynamics into modes oscillating at a single frequency. These modes are associated with different energy levels and spatial structures. The approach  is illustrated using time-resolved PIV data of a cylinder wake flow with associated Reynolds number equal to 3,900.","palabras_clave":"Koopman analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63299,"idioma_id":4,"titulo":"Classical transients and the support of open quantum maps","resumen":"The basic ingredients in a semiclassical theory are the classical invariant objects serving as a support forquantization. Recent studies, mainly obtained on quantum maps, have led to the commonly accepted belief thatthe classical repeller\u2014the set of nonescaping orbits in the future and past evolution\u2014is the object that suitablyplays this role in open scattering systems. In this paper we present numerical evidence warning that this maynot always be the case. For this purpose we study recently introduced families of tribaker maps [L. Ermann,G. G. Carlo, J. M. Pedrosa, and M. Saraceno, Phys. Rev. E 85, 066204 (2012)], which share the same asymptoticproperties but differ in their short-time behavior. We have found that although the eigenvalue distribution of theevolution operator of these maps follows the fractal Weyl law prediction, the theory of short periodic orbits foropen maps fails to describe the resonance eigenfunctions of some of them. This is a strong indication that newelements must be included in the semiclassical description of open quantum systems. We provide an interpretationof the results in order to have hints about them.","palabras_clave":"quantum chaos|resonances|fractal Weyl law"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63300,"idioma_id":4,"titulo":"Spectral properties of Google matrix of Wikipedia and other networks","resumen":"We study the properties of eigenvalues and eigenvectors of the Google matrix of the Wikipediaarticles hyperlink network and other real networks. With the help of the Arnoldi method, we analyzethe distribution of eigenvalues in the complex plane and show that eigenstates with significant eigenvaluemodulus are located on well defined network communities. We also show that the correlator betweenPageRank and CheiRank vectors distinguishes different organizations of information flow on BBC and LeMonde web sites.","palabras_clave":"Complex Networks|World Wide Web|Google Matrix|Wikipedia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63301,"idioma_id":4,"titulo":"Effect of moisture content on some physical properties of barley","resumen":"Physical properties of barley (Hordeum vulgare L.) seeds were determined as a function of moisture content.Several properties were studied in the moisture range from 13.15 to 45.82% dry basis, the lengthincreased from 8.10 to 8.62 mm, the width from 3.42 to 3.74 mm, the thickness from 2.61 mm to 2.84 mm,the arithmetic and geometric diameter of seeds from 4.71 to 5.07 mm and from 4.16 to 4.50 mm, respectively.Besides, the 1000-seed mass increased from 39.46 to 51.13 g, the surface area increased from 54.60to 63.79 mm2, the porosity increased from 42.69% to 44.44%, the angle of repose increased from 18.18° to27.31°, and the static coefficient of friction showed an increase of 60.17%, 38.41% and 59.35% for the surfacesof plywood, galvanized steel and aluminum, respectively. From the experimental determinations itcould be observed that only the apparent and true density decreased from 699.38 to 647.04 kg m&#8722;3 andfrom 1220.30 to 1164.62 kg m-3 respectively. In the range of evaluated moisture, all these properties ?except length ? showed moisture dependence according to linear relationships; the length exhibited abehavior of second-order polynomial. Finally, the sphericity behaves differently presenting a maximumof 53.15% for a moisture content of 35.29%.","palabras_clave":"BARLEY|PHYSICAL PROPERTIES|MOISTURE CONTENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63303,"idioma_id":4,"titulo":"Application of hairy roots for phytoremediation: what makes them an interesting tool for this purpose?","resumen":"In recent years, hairy roots (HRs) have been  successfully used as research tools for screening the potentialities  of different plant species to tolerate, accumulate,  and\/or remove environmental pollutants, such as PCBs,  TNT, pharmaceuticals, textile dyes, phenolics, heavy metals,  and radionuclides. This is in part due to several advantages  of this plant model system and the fact that roots have  evolved specific mechanisms to deal with pollutants because  they are the first organs to have contact with them.  In addition, by using HRs some metabolic pathways and  enzymatic catalyzed reactions involved in pollutants detoxification  can be elucidated as well as the mechanisms of  uptake, transformation, conjugation, and compartmentation  of pollutants in vacuoles and\/or cell walls, which are important  detoxification sites in plants. Plant roots also stimulate  the degradation of contaminants by the release of root exudates  and oxido-reductive enzymes, such as peroxidases  (Px) and laccases, that are associated with the removal of  some organic pollutants. HRs are also considered good  alternatives as enzyme sources for remediation purposes.  Furthermore, application of genetic engineering methods  and development of microbe-assisted phytoremediation are  feasible strategies to enhance plant capabilities to tolerate,  accumulate, and\/or metabolize pollutants and, hence, to  create or find an appropriate plant system for environmental  cleanup. The present review highlights current knowledge,  recent progress, areas which need to be explored, and future  perspectives related to the application and improvement of  the efficiency of HRs for phytoremediation research.","palabras_clave":"Hairy roots|Phenol|Phytoremediation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63304,"idioma_id":4,"titulo":"High reactivity of nitric oxide with peroxo groups on BaO particles. DFT calculations","resumen":"The reactivity of NO with peroxo groups  present on Ba6O6 particles is studied using the density functional theory (DFT). Bulk-like and hexagonal structures were considered. The surface peroxo groups were modeled by adding an oxygen atom (Oa) to a structural oxygen anion (Os). Calculations indicate that the NO molecule can react either with Os or, with Oa to form NO2 by surpassing an activation barrier of only 0.2 eV or less. In particular, the NO2 formation via the extraction of Os involves a transition state in which an interchange is produced between Oa and Os. The predicted high reactivity is in agreement with experiments performed on highly defective samples of BaO wherein NO activation is observed to occur on surface peroxo groups.","palabras_clave":"NO|BaO PARTICLE|PEROXO|NOx STORAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63305,"idioma_id":4,"titulo":"AN APPLICATION OF THE MAXIMUM ENTROPY METHOD WITH SMOOTHING TO INDUSTRIAL TOMOGRAPHY","resumen":"The maximum entropy method combined with a smoothing restriction was applied to the tomography of a BNC connector using five noisy radiographies from different rotating views. A heuristic control of the filter parameter is constructed by combining the projection error and the global smoothness. The proposed algorithm can be used in cases where the homogeneity of internal material is a priori known from the design and manufacturing processes.","palabras_clave":"Tomografía computada|Reconstrucción de imágenes|Máxima entropía"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63306,"idioma_id":4,"titulo":"Detection and Characterization of Phospholipase A2 (PLA2) in Caiman latirostris and Caiman yacare Plasma","resumen":"Reptiles have proven to have a versatile and efficient nonspecific immune system adapted to the environments in which they commonly live. Phospholipase A2 (PLA2) is important hydrolytic enzyme involved in the regulation of specific types of messengers, with significant roles in the  innate immune response. A number of agents that exert effects on cellular receptors emit a series of signals leading to the increased activity of PLA2. Phospholipase A2 has been identified and characterized  in temperature, plasma concentration, and kinetic dependence in two species of caiman.  The results of these studies suggest that the high PLA2 activities observed in caiman plasma may be an important component of a well-developed innate immunity. Based on the knowledge of their properties, this powerful immunologic component should be evaluated as a possible application in the veterinary or even human therapeutic industry. Additionally, this is another reason to consider these animals excellent models for the study of immune phylogenetic mechanisms.","palabras_clave":"PHOSPHOLIPASE| PLA2|IMMUNE SYSTEM|CROCODILIANS|INNATE IMMUNITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63307,"idioma_id":4,"titulo":"Moderate and severe perinatal asphyxia induces differential effects on cocaine sensitization in adult rats","resumen":"Perinatal asphyxia (PA) increases the likelihood of suffering from dopamine-related disorders, such as ADHD and schizophrenia. Since dopaminergic transmission plays a major role in cocaine sensitization, the purpose of this study was to determine whether PA could be associated with altered behavioral sensitization to cocaine. To this end, adult rats born vaginally (CTL), by caesarean section (C+), or by C+ with 15 min (PA15, moderate PA) or 19 min (PA19, severe PA) of global anoxia were repeatedly administered with cocaine (i.p., 15 mg\/kg) and then challenged with cocaine (i.p., 15 mg\/kg) after a five-day withdrawal period. In addition, c-Fos, FosB\/&#916;FosB, DAT and TH expression were assessed in dorsal (CPu) and ventral (NAcc) striatum. Results indicated that PA15 rats exhibited an increased locomotor sensitization to cocaine, while PA19 rats displayed an abnormal acquisition of locomotor sensitization and did not express a sensitized response to cocaine. c-Fos expression in NAcc, but not in CPu, was associated with these alterations in cocaine sensitization. FosB\/&#916;FosB expression was increased in all groups and regions after repeated cocaine administration, although it reached lower expression levels in PA19 rats. In CTL, C+ and PA15, but not in PA19 rats, the expression of TH in NAcc was reduced in groups repeatedly treated with cocaine, independently of the challenge test. Furthermore, this reduction was more pronounced in PA15 rats. DAT expression remained unaltered in all groups and regions studied. These results suggest that moderate PA may increase the vulnerability to drug abuse and in particular to cocaine addiction.","palabras_clave":"PERINATAL ASPHYXIA|COCAINE SENSITIZATION|C-FOS|DELTAFOSB|TYROSINE HYDROXYLASE|DOPAMINE TRANSPORTER|CAUDATE-PUTAMEN|NUCLEUS ACCUMBENS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63308,"idioma_id":4,"titulo":"A land cover map of Latin America and the Caribbean in the framework of the SERENA project","resumen":"Land cover maps at different resolutions and mapping extents contribute   to modeling and support decision making processes. Because land cover   affects and is affected by climate change, it is listed among the 13   terrestrial essential climate variables. This paper describes the   generation of a land cover map for Latin America and the Caribbean (LAC)   for the year 2008. It was developed in the framework of the project   Latin American Network for Monitoring and Studying of Natural Resources   (SERENA), which has been developed within the GOFC-GOLD Latin American   network of remote sensing and forest fires (RedLaTIF). The SERENA land   cover map for LAC integrates: 1) the local expertise of SERENA network   members to generate the training and validation data, 2) a methodology   for land cover mapping based on decision trees using MODIS time series,   and 3) class membership estimates to account for pixel heterogeneity   issues. The discrete SERENA land cover product, derived from class   memberships, yields an overall accuracy of 84% and includes an   additional layer representing the estimated per-pixel confidence. The   study demonstrates in detail the use of class memberships to better   estimate the area of scarce classes with a scattered spatial   distribution. The land cover map is already available as a printed wall   map and will be released in digital format in the near future. The   SERENA land cover map was produced with a legend and classification   strategy similar to that used by the North American Land Change   Monitoring System (NALCMS) to generate a land cover map of the North   American continent, that will allow to combine both maps to generate   consistent data across America facilitating continental monitoring and   modeling.","palabras_clave":"Land cover classification|Decision trees|CEREGEO|MODIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63309,"idioma_id":5,"titulo":"'Seguridad y soberanía alimentaria en Argentina'","resumen":"En el articulo se proponen algunas líneas de reflexión entorno a la situación actual de Argentina respecto de la seguridad y lasoberanía alimentaria a partir de que el país vive la paradojal y críticasituación de ser reconocido como proveedor de alimentos y simultáneamentemostrar parte de su población con porcentajes de desnutrición yhambre. Consideramos que los planteos en torno a formas agrícolas de producciónalternativa, soberanía alimentaria, y función social de la tierra,permiten en primer lugar poner en discusión al sistema agroproductivodominante.","palabras_clave":"SOBERANÍA|SEGURIDAD|ALIMENTACIÓN|PRODUCCIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63310,"idioma_id":5,"titulo":"Edad mínima de exposición de superficies en canteras-taller: reflexiones en torno a las primeras dataciones mediante microlaminaciones del barniz de las rocas (VML) para el noroeste argentino","resumen":"El objetivo de este trabajo es   presentar los resultados de las primeras dataciones del barniz de las   rocas para el Noroeste argentino, obtenidas mediante&nbsp; la técnica VML   (varnish microlamination) y discutir su utilidad a partir de un caso de   estudio localizado en el valle de Santa María. El VML brinda una   estimación de la edad mínima de exposición de las superficies geológicas   y estructuras arqueológicas (clastos, rodados y artefactos) y es   diferente e independiente de métodos como el carbono 14. Se recolectaron   y analizaron conjuntos líticos superficiales ?geológicos y   arqueológicos- del sitio arqueológico Planchada La Puntilla (PLP), cuyas   dataciones sugieren una edad mínima de exposición de la superficie   entre los 6.500-7.300 años a.p. y una edad mínima para las piezas   líticas del sitio Planchada La Puntilla de 6.500-5.900 años a.p..   Además, la secuencia de microlaminaciones del barniz conforman un   registro climático, que hacen del barniz una herramienta única para   discutir el cambio climático del pasado en un área donde ha sido difícil   encontrar proxies más tradicionales.&nbsp; Los resultados de VML permitieron   discutir la temporalidad de los procesos de producción artefactual   lítica y sus implicancias para otros sitios de superficie,   tradicionalmente asociados a la denominada Industria Ampajango, así como   las variaciones climáticas durante el Holoceno medio y tardío.","palabras_clave":"ARQUEOLOGIA|CONJUNTOS LITICOS DE SUPERFICIE|MICROLAMINACION DEL BARNIZ VML|GEOCRONOLOGIA|NOROESTE ARGENTINO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63311,"idioma_id":4,"titulo":"Dam Methylation Participates in the Regulation of PmrA\/ PmrB and RcsC\/RcsD\/RcsB Two Component Regulatory Systems in Salmonella enterica Serovar Enteritidis","resumen":"The absence of Dam in Salmonella enterica serovar Enteritidis causes a defect in lipopolysaccharide (LPS) pattern associated  to a reduced expression of wzz gene. Wzz is the chain length regulator of the LPS O-antigen. Here we investigated whether  Dam regulates wzz gene expression through its two known regulators, PmrA and RcsB. Thus, the expression of rcsB and  pmrA was monitored by quantitative real-time RT-PCR and Western blotting using fusions with 36FLAG tag in wild type (wt)  and dam strains of S. Enteritidis. Dam regulated the expression of both rcsB and pmrA genes; nevertheless, the defect in LPS  pattern was only related to a diminished expression of RcsB. Interestingly, regulation of wzz in serovar Enteritidis differed  from that reported earlier for serovar Typhimurium; RcsB induces wzz expression in both serovars, whereas PmrA induces  wzz in S. Typhimurium but represses it in serovar Enteritidis. Moreover, we found that in S. Enteritidis there is an interaction  between both wzz regulators: RcsB stimulates the expression of pmrA and PmrA represses the expression of rcsB. Our results  would be an example of differential regulation of orthologous genes expression, providing differences in phenotypic traits  between closely related bacterial serovars.","palabras_clave":"DAM METHYLATION |Lipopolysaccharide (LPS)|Salmonella enterica Serovar Enteritidis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63312,"idioma_id":4,"titulo":"Toll-like receptor 2 ligands promote microglial cell death by inducing autophagy","resumen":"Microglial cells are phagocytes in the central nervous system (CNS) and become activated in pathological conditions, resulting in microgliosis, manifested by increased cell numbers and inflammation in the affected regions. Thus, controlling microgliosis is important to prevent pathological damage to the brain. Here, we evaluated the contribution of Toll-like receptor 2 (TLR2) to microglial survival. We observed that  activation of microglial cells with peptidoglycan (PGN) from Staphylococcus aureus and other TLR2 ligands results in cell activation followed by the induction of autophagy and autophagy-dependent cell death. In C57BL\/6J mice, intracerebral injection of PGN increased  the autophagy of microglial cells and reduced the microglial\/macrophage cell number in brain parenchyma. Our results demonstrate a novel role of TLRs in the regulation of microglial cell activation and survival,  which are important for the control of microgliosis and associated inflammatory responses in the CNS.","palabras_clave":"inflammation|peptidoglycan|CNS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63313,"idioma_id":4,"titulo":"The assessment of a smart anticorrosive coating by the electrochemical noise technique","resumen":"Zinc phosphate and related compounds are convenient replacements for chromates. However, more eco-compatible pigments are being investigated. The objective of this research was to develop a modified zeolitic rock which is intended to replace phosphate pigments in anticorrosive paints. The modified zeolitic rock was obtained by grinding the rock followed with ionic exchange with molybdenyl ions. This ?composite? has an intelligent behavior because molybdenum compounds are leached from the zeolite particle by corrodent species. The anticorrosive properties of this zeolitic rock were studied by electrochemical techniques, employing inhibitor suspensions, and formulating anticorrosive coatings. Coatings  performance was evaluated by accelerated tests (humidity chamber and salt spray) and electrochemical noise measurements (ENM). Electrochemical noise data were analyzed in the time domain. The noise resistance (Rn) was compared, as far as possible, with the polarization resistance.  It was demonstrated that zinc phosphate content could be reduced to one-third with respect to the recommended value in the literature. The electrochemical noise technique allowed to differentiate the anticorrosive performance of the different coatings formulated in this research.","palabras_clave":"ZINC PHOSPHATE|ZEOLITES|MOLYBDENUM IONS|ELECTROCHEMICAL NOISE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63314,"idioma_id":4,"titulo":"The application of bioactive compounds from the food industry to control mould growth in indoor waterborne coatings","resumen":"Microbial growth in indoor environments creates health problems, especially in people with asthma; approximately 80% of these patients are allergic to mold. Antimicrobial coatings are formulated to generate surfaces that are easy to clean and may also incorporate active agents, commonly called biocides, which inhibit microbial colonization, subsequent growth and bio-deterioration of the substrates.  Some research lines seek to replace traditional organometallic and organochlorines biocides with environmentally acceptable ones. The aim of this research was, primarily, to explore the possible application of different compounds used in food industry like preservatives to be used as antimicrobial additives for antimicrobial coatings. Four biocides were tested against two different ambient moulds isolated from an interior painted wall (Chaetomium globosum and Alternaria alternate). The selected biocides were zinc salicylate, zinc benzoate, calcium benzoate and potassium sorbate. The resulting paints were subjected to biological and physical tests (viscosity, hiding power, humidity absorption and biocides leaching rate). Bioassays revealed that zinc benzoate and zinc salicylate resulted active against both fungi.","palabras_clave":"ANTIMICROBIAL COATING|MOLD|BIODETERIORATION|BIOCIDE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63315,"idioma_id":5,"titulo":"LA IDENTIDAD POLÍTICA DEL MAS-IPSP EN BOLIVIA. DE TRADICIONES, DEMANDAS Y ANTAGONISMOS","resumen":"En este artículo se pretende analizar al MAS-IPSP en tanto identidad política que emergió en resistencia a la férrea aplicación de políticas neoliberales en Bolivia y se consolidó como una alternativa popular que triunfó en las elecciones nacionales de diciembre de 2005 y de diciembre de 2009. El análisis aquí propuesto arroja importantes claves para comprender el proceso boliviano actual, planteando novedosas articulaciones entre \u201Clo social\u201D y \u201Clo político\u201D.","palabras_clave":"IDENTIDAD|DEMANDAS|FRONTERAS|TRADICIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63316,"idioma_id":5,"titulo":"Las Múltiples Identidades Políticas en la Refundación Institucional  Boliviana","resumen":"El presente artículo rescata la noción de identidades políticas para analizar el actual proceso político boliviano. En ese sentido se plantean articulaciones entre la consolidación de los sindicatos de productores de coca, el ciclo de acción colectiva de los primeros años del siglo XXI, el crecimiento del MAS-IPSP y la llegada al gobierno de Evo Morales. El énfasis se coloca en las implicancias de esta articulación para la refundación institucional del país, expresada - entre otros factores- a través de la sanción de una nueva constitución política del Estado.(Indexação: Clase - Citas Latinoamericanas en Ciencias Sociales y Humanidades).","palabras_clave":"IDENTIDAD POLITICA|ESTADO|BOLIVIA|DEMOCRACIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63317,"idioma_id":4,"titulo":"Effect of the interface on the photophysics of eosin-Y in reverse miceles","resumen":"The photophysics of the xanthene dye eosin-Y (Eos) was studied in reverse micelles (RMs) of sodium 1,4-bis (2-ethylhexyl) sulfosuccinate (AOT) and benzylhexadecyldimethylammonium chloride (BHDC). Special interest was assigned to the effect of organization on the triplet state properties. In the reversemicellar media both the absorption and emission spectra of the dye present a red shift with respect to water. It is concluded that Eos in both reverse micellar systems is localized in the interface. Although this is predictable in BHDC due to the positive charge at the interface and the negative charge of the dye, it is notable in the case of the negative interface of AOT. In this case it may be concluded that Eos resides in the interface co-micellizing with the surfactant, although more exposed to water than in the case of BHDC. Triplet state decay kinetics and absorption spectra were determined by laser flash photolysis. The triplet lives longer in RMs than in homogeneous solvents. The compartmentalization of the dye precludes the auto-quenching which shorts the lifetime in homogeneous solvents. The quenching by aliphatic amines was also investigated. The hydrophilic triethanolamine (TEOA) is localized in the water pool, and the triplet quenching is more efficient than in homogeneous solvents. On the other hand the quenching by tributylamine (TBA) which is located in the bulk organic solvent is much less efficient. The quenching by TEOA in RMs can be understood in terms of an exchange mechanism between micelles. Quantum yieldsof radicals produced in the triplet quenching process were determined.","palabras_clave":"Eosin-Y|Photophysics|Triplet state|Reverse micelles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63318,"idioma_id":4,"titulo":"From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments","resumen":"Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by \u201Ceffective diffusion coefficients\u201D that depend on both the reaction rates and the \u201Cfree\u201D diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.","palabras_clave":"FLUORESCENCE|CORRELATION|SPECTROSCOPY|DIFFUSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63319,"idioma_id":4,"titulo":"Visible light photopolymeriza-tion in BHDC reverse micelles. Laser flash photolysis study of the photoinitiating mechanism","resumen":"The photopolymerization of acrylamide (AA) in reverse micelles (RMs) of benzyl hexadecyl dimethylammonium chloride (BHDC) was investigated. The polymerization was performed by irradiation in the 500 nm region of the spectrum, employing as photoinitiating system the synthetic dye safranine-O and triethanolamine (TEOA) as co-initiator. The characterization of the nanoparticles of polyacrylamide wasdone by dynamic light scattering. Molecular weight determinations were carried out by viscosimetry. Small nanoparticles of polyacrylamide are formed with a low polydispersity and a molecular weight close to 106. The results were compared with those in AOT (sodium bis(2-ethylhexyl) sulfosuccinate) RMs. Laser flash photolysis was used to investigate the photoinitiating mechanism. The maximum of the T?T absorption spectra in RMs confirms that, in spite of its positive charge, the dyes remain at the interface co-micellizing with BHDC. The triplet lifetime is much longer in the RMs than in homogeneous organic solvents. The triplet quenching by TEOA was very much more effective in the RMs than in a homogeneous solvent. The quantum yield of formation of active radicals was evaluated from the transient absorption spectra. It was concluded that they are generated by an intermicellar electron transfer process followed by an intramicellar proton transfer reaction. The quantum yield was found to be 0.2±0.02. A similar radical yield in a homogeneous solvent requires an amine concentration near 50 times higher.","palabras_clave":"visible photopolymerization|Safranine|Reverse micelles|Triplet quenching"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63320,"idioma_id":4,"titulo":"Endothelin 1 and 3 induce choleresis in the rat through ETB receptors coupled to nitric oxide and vago-vagal reflexes","resumen":"We have reported previously that centrally applied ET (endothelin)-1 and ET-3 induce either choleresis or cholestasis  depending on the dose. In the present study, we sought to establish the role of these endothelins in the short-term  peripheral regulation of bile secretion in the rat. Intravenously infused endothelins induced significant choleresis in  a dose-dependent fashion, ET-1 being more potent than ET-3. Endothelins (with the exception of a higher dose of  ET-1) did not affect BP (blood pressure), portal venous pressure or portal blood flow. ET-1 and ET-3 augmented the  biliary excretion of bile salts, glutathione and electrolytes, suggesting enhanced bile acid-dependent and  -independent bile flows. ET-induced choleresis was mediated by ETB receptors coupled to NO and inhibited by  truncal vagotomy, atropine administration and capsaicin perivagal application, supporting the participation of  vagovagal reflexes. RT (reverse transcription)?PCR and Western blot analysis revealed ETA and ETB receptor  expression in the vagus nerve. Endothelins, through ETB receptors, augmented the hepatocyte plasma membrane  expression of Ntcp (Na+ \/taurocholate co-transporting polypeptide; Slc10a1), Bsep (bile-salt export pump; Abcb11),  Mrp2 (multidrug resistance protein-2; Abcc2) and Aqp8 (aquaporin 8). Endothelins also increased the mRNAs of  these transporters. ET-1 and ET-3 induced choleresis mediated by ETB receptors coupled to NO release and  vagovagal reflexes without involving haemodynamic changes. Endothelin-induced choleresis seems to be caused by  increased plasma membrane translocation and transcriptional expression of key bile transporters. These findings  indicate that endothelins are able to elicit haemodynamic-independent biological effects in the liver and suggest  that these peptides may play a beneficial role in pathophysiological situations where bile secretion is impaired.","palabras_clave":"ENDOTELINAS |ÓXIDO NÍTRICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63321,"idioma_id":4,"titulo":"Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis","resumen":"Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials\/Methods. Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w\/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results. A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions. Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.","palabras_clave":"WATER CHANNEL|RENAL FUNCTION|THYROID HORMONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63322,"idioma_id":4,"titulo":"Risk Assessment on Irrigation of Vitis vinifera L. cv Malbec with Hg Contaminated Waters","resumen":"Concerns regard watering crops with Hg  contaminated waters have arisen worldwide recently. In these  sense Hg uptake by Vitis vinifera L. cv. Malbec was evaluated  under greenhouse conditions by the administration of Hg2+ for  4 days through irrigation water (short-term administration).  Vines uptake Hg translocating it from roots through stems to  leaves. Roots accumulated the higher Hg concentration. Hg in  stems and leaves was accumulated mostly as organic Hg, bind  to different moieties. Size exclusion chromatography (SEC)  and ion pair chromatography (IPC) were employed to reach  insights into these ligands. Hg is distributed mainly in high  molecular weight fractions of 669 kDa in vine plants. In stems  and leaves, Hg&#8722;S associations were found in 669 and 66 kDa  fractions. Hg&#8722;S association at 66 kDa suggests a possible protein or peptide binding affecting vines normal physiology. Since Hg  contamination through organomercurials is more harmful than Hg2+ itself, methyl mercury, dimethyl mercury, and phenyl  mercury, more toxic Hg species were evaluated with negative results.","palabras_clave":"Contaminated water|Dimethyl mercury|Greenhouse conditions|Hg concentrations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63323,"idioma_id":4,"titulo":"Spectral Characteristics and Photosensitization of TiO2 Nanoparticles in Reverse Micelles by Perylenes","resumen":"We report on the photosensitization of titanium dioxide nanoparticles (TiO2 NPs) synthesized inside AOT (bis(2-ethylhexyl) sulfosuccinate sodium salt) reverse micelles following photoexcitation of perylene derivatives with dicarboxylate anchoring groups. The dyes, 1,7-dibromoperylene-3,4,9,10-tetracarboxy dianhydride (1), 1,7-dipyrrolidinylperylene-3,4,9,10-tetracarboxy dianhydride (2), and 1,7-bis(4-tert-butylphenyloxy)perylene-3,4,9,10-tetracarboxy dianhydride (3), have considerably di&#64256;erent driving forces for photoinduced electron injection into the TiO2 conduction band, as estimated by electrochemical measurements and quantum mechanical calculations. Fluorescence anisotropy  measurements indicate that dyes 1 and 2 are preferentially solubilized in the micellar structure, creating a relatively large local concentration that favors the attachment of the dye to the TiO2 surface. The binding process was followed by monitoring the hypsochromic shift of the dye absorption spectra over time for 1 and 2. Photoinduced electron transfer from the singlet excited state of 1 and 2 to the TiO2 conduction band (CB) is indicated by emission quenching of the TiO2 -bound form of the  dyes and con&#64257;rmed by transient absorption measurements of the radical cation of the dyes and free carriers (injected electrons) in the TiO2  semiconductor. Steady state and transient spectroscopy indicate that dye 3 does not bind to the TiO2 NPs and does not photosensitize the semiconductor. This observation was rationalized as a consequence of the bulky t-butylphenyloxy groups which create a strong steric impediment for deep access of the dye within the micelle structure to reach the semiconductor oxide surface.","palabras_clave":"Nanoparticles |TiO2|Reverse micelles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63324,"idioma_id":4,"titulo":"Evaluation of total white blood cell count as a marker for proviral load of  bovine leukemia virus in dairy cattle from herds with a high seroprevalence of  antibodies against bovine leukemia virus.","resumen":"OBJECTIVE: To determine the reference interval for WBC counts in Holstein dairy  cows from herds with high seroprevalence for anti-bovine leukemia virus (BLV)   antibodies, analyze the correlation of total WBC counts and blood proviral load   (bPVL) in BLV-infected animals, and determine whether total WBC count can be used    a hematologic marker for in vivo infection. ANIMALS: 307 lactating cows from 16  dairy herds with high BLV seroprevalence. PROCEDURES: Blood samples were   collected for assessment of plasma anti-BLV p24 antibody concentration (all cows), manual determination of WBC count (161 BLV-seronegative cows from 15 herds), and evaluation of bPVL (146 cows from another herd). RESULTS: The WBC      count reference interval (ie, mean +\/ 2 SD) for BLV-seronegative dairy cows was 2,153 to 11,493 cells\/muL. Of the 146 cows used to analyze the correlation  between WBC count and bPVL, 107 (73%) had WBC counts within the reference  interval; of those cows, only 21 (19.6%) had high bPVL. Most cows with high WBC  counts (35\/39) had high bPVL. Mean WBC count for cows with high bPVL was  significantly higher than values for cows with low or undetectable bPVL. White  blood cell counts and bPVL were significantly (rho = 0.71) correlated. CONCLUSIONS AND CLINICAL RELEVANCE: These data have provided an updated reference      interval for WBC counts in Holstein cows from herds with high BLV seroprevalence.   In dairy cattle under natural conditions, WBC count was correlated with bPVL;  thus, WBC count determination could be a potential tool for monitoring BLV  infection levels in attempts to control transmission.","palabras_clave":"BLV|proviral load|white blood cells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63325,"idioma_id":4,"titulo":"Hepatocellular alterations and dysregulation of oncogenic pathways in the liver of transgenic mice overexpressing growth hormone","resumen":"Growth hormone (GH) overexpression throughout life in transgenic mice is associated with the development of liver tumors at old ages. The preneoplastic pathology observed in the liver of young adult GH-overexpressing mice is similar to that present in humans at high risk of hepatic cancer. To elucidate the molecular pathogenesis underlying the pro-oncogenic liver pathology induced by prolonged exposure to elevated GH levels, the activation and expression of several components of signal transduction pathways that have been implicated in hepatocellular carcinogenesis were evaluated in the liver of young adult GH-transgenic mice. In addition, males and females were analyzed in parallel in order to evaluate sexual dimorphism. Transgenic mice from both sexes exhibited hepatocyte hypertrophy with enlarged nuclear size and exacerbated hepatocellular proliferation, which were higher in males. Dysregulation of several oncogenic pathways was observed in the liver of GH-overexpressing transgenic mice. Many signaling mediators and effectors were upregulated in transgenic mice compared with normal controls, including Akt2, NF&#954;B, GSK3&#946;, &#946;-catenin, cyclin D1, cyclin E, c-myc, c-jun and c-fos. The molecular alterations described did not exhibit sexual dimorphism in transgenic mice except for higher gene expression and nuclear localization of cyclin D1 in males. We conclude that prolonged exposure to GH induces in the liver alterations in signaling pathways involved in cell growth, proliferation and survival that resemble those found in many human tumors.","palabras_clave":"transgenic mice|growth hormone|heart|tumor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63326,"idioma_id":4,"titulo":"Optimization of benzyloxazoles as non-nucleoside inhibitors of HIV-1 reverse transcriptase to enhance Y181C potency","resumen":"Design of non-nucleoside inhibitors of HIV-1 reverse transcriptase with improved activity towards Tyr181Cys containing variants was pursued with the assistance of free energy perturbation (FEP) calculations. Optimization of the 4-R substituent in 1 led to ethyl and isopropyl analogs 1e and 1f with 1\u20137 nM potency towards both the wild-type virus and a Tyr181C variant","palabras_clave":"Anti-HIV agents|Computer-aided drug design|Free-energy calculations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63327,"idioma_id":5,"titulo":"Influencia de las condiciones de incubación en la  detección de anticuerpos contra el Virus de la leucosis bovina por ELISA","resumen":"En este trabajo se modificaron las condiciones de incubación del ensayo de ELISAp24 que detecta anticuerpos contra el Virus de la Leucosis bovina, con el propósito de evaluar si se reduce el número de resultados falsos y en consecuencia la factibilidad de usarlo con fines diagnósticos confirmatorios, ya que fue desarrollado y validado en nuestro laboratorio para el análisis y monitoreo de seroprevalencia a nivel rodeo y\/o para tamizaje de muestras individuales. Se analizaron 128 muestras de suero pertenecientes a 2 rodeos infectados naturalmente de distinto sistema productivo y prevalencia individual, utilizando las condiciones estándar (20-25ºC-30 minutos) y las modificadas (4-8ºC-16-24hs). Los resultados evidencian que las condiciones modificadas no sólo mejoran la capacidad de detección de individuos infectados, sino que disminuyen el número de resultados falsos positivos. Estos hallazgos evidencian la factibilidad de utilizar el ensayo de ELISAp24 como método confirmatorio, y abren camino hacia la realización de un protocolo integral de validación para evaluar su performance diagnóstica, ya que la mejora independiente de la sensibilidad y la especificidad resulta de importancia crítica cuando se utiliza un ensayo para determinar el estado sanitario individual.                                       ","palabras_clave":"BLV|ELISA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63328,"idioma_id":4,"titulo":"Invasion status of Asiatic red-bellied squirrels in Argentina","resumen":"The only known introduction of a squirrel species into South America is the case of the Asiatic red-bellied tree squirrel Callosciurus erythraeus that was introduced in the Pampas Region of Argentina in 1970. To inform management programmes, we analysed the current distribution, expansion rate, and abundance of the red-bellied squirrel in Argentina, and identified invasion pathways. Apart from the first and main focus of invasion, three other invasion foci have originated as a consequence of intentional releases between 1995 and 2000. The main invasion focus already extends over >1300 km2, where estimated density averaged 15 ind\/ha and numbers of squirrels may reach 100000 individuals. The area invaded in the other three invasion foci varied between 2 and mean densities were around 3-5 ind\/ha. Squirrel abundance and residents? attitude towards this introduced species in the main invasion focus make eradication unfeasible, though management actions such as control, containment and mitigation of damages should be undertaken. Eradication of the relatively small invasion foci must be immediately evaluated, with priority given to valuable conservation areas under high invasion risk.  ","palabras_clave":"Callosciurus erythraeus|Abundance and distribution|Pathways of invasion|Invasive species|Landscape fragmentation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63329,"idioma_id":4,"titulo":"Optimization of diarylazines as anti-HIV agents with dramatically","resumen":"Non-nucleoside inhibitors of HIV-1 reverse transcriptase are reported that have ca. 100-fold greater solubilitythan the structurally related drugs etravirine and rilpivirine, while retaining high anti-viral activity.The solubility enhancements come from strategic placement of a morpholinylalkoxy substituent inthe entrance channel of the NNRTI binding site. Compound 4d shows low-nanomolar activity similarto etravirine towards wild-type HIV-1 and key viral variants4d shows low-nanomolar activity similarto etravirine towards wild-type HIV-1 and key viral variants","palabras_clave":"Anti-HIV agent|NNRTI|Drug solubility"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63330,"idioma_id":4,"titulo":"Improvement of the boron neutron capture therapy (BNCT) by the previous administration of the histone deacetylase inhibitor sodium butyrate  for the treatment of thyroid carcinoma","resumen":"Abstract We have shown that boron neutron capture  therapy (BNCT) could be an alternative for the treatment of  poorly differentiated thyroid carcinoma (PDTC). Histone  deacetylase inhibitors (HDACI) like sodium butyrate (NaB)  cause hyperacetylation of histone proteins and show capacity  to increase the gamma irradiation effect. The purpose of  these studies was to investigate the use of the NaB as a  radiosensitizer of the BNCT for PDTC. Follicular thyroid  carcinoma cells (WRO) and rat thyroid epithelial cells  (FRTL-5) were incubated with 1 mM NaB and then treated  with boronophenylalanine 10BPA (10 lg 10B ml-1) ? neutrons,  or with 2, 4-bis (a,b-dihydroxyethyl)-deutero-porphyrin  IX 10BOPP (10 lg10B ml-1) ? neutrons, or with aneutron beam alone. The cells were irradiated in the thermal  column facility of the RA-3 reactor (flux = (1.0 ± 0.1) 9  1010 n cm-2 s-1). Cell survival decreased as a function of  the physical absorbed dose in both cell lines. Moreover, the  addition of NaB decreased cell survival (p&#65533;.05) in WRO  cells incubated with both boron compounds. NaB increased  the percentage of necrotic and apoptotic cells in both BNCT  groups (p&#65533;.05). An accumulation of cells in G2\/M phase  at 24 h was observed for all the irradiated groups and the  addition of NaB increased this percentage. Biodistribution  studies of BPA (350 mg kg-1 body weight) 24 h after NaB  injection were performed. The in vivo studies showed that  NaB treatment increases the amount of boron in the tumor at2-h post-BPA injection (p&#65533;.01). We conclude that NaB  could be used as a radiosensitizer for the treatment of thyroid  carcinoma by BNCT.","palabras_clave":"Sodium butyrate |Radiosensitizer|Thyroid|cancer|BNCT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63331,"idioma_id":4,"titulo":"Optimal dual frames and frame completions for majorization","resumen":"In this paper we consider two problems in frame theory. On the one hand, given a set of vectors F we describe the spectral and geometrical structure of optimal completions of F by a finite family of vectors with prescribed norms, where optimality is measured with respect to majorization. In particular, these optimal completions are the minimizers of a family of convex functionals that include the mean square error and the Benedetto?Fickus&#700; frame potential. On the other hand, given a fixed frame F we describe explicitly the spectral and geometrical structure of optimal frames G that are in duality with F and such that the Frobenius norms of their analysis operators is bounded from below by a fixed constant. In this case, optimality is measured with respect to submajorization of the frames operators. Our approach relies on the description of the spectral and geometrical structure of matrices that minimize submajorization on sets that are naturally associated with the problems above.","palabras_clave":"DUAL FRAMES|FRAME COMPLETIONS|MAJORIZATION|SCHUR-HORN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63332,"idioma_id":5,"titulo":"Tolerancia a la desecación de semillas de Prosopis ferox y Pterogyne nitens (Fabaceae)","resumen":"Desiccation tolerance in seeds of Prosopis ferox and Pterogyne nitens (Fabaceae). The high number of endemisms and species diversity together with the accelerated biodiversity loss by deforestation, especially in North Western Argentina, points out the need to work on species conservation combining ex situ and in situ strategies. The aim of this work was to study the desiccation tolerance in seeds of P. ferox and P. nitens for long term ex situ conservation at the Germplasm Bank of Native Species (BGEN) of the National University of Salta (Argentina). The fruits were collected from ten individuals in P. ferox at the National Park Los Cardonesand from two sites (Orán and Rivadavia) for P. nitens. Desiccation tolerance was assessed following previous established methodologies. The moisture content (MC) of the seeds was determined by keeping them in oven at 103°C and weighting the samples at different intervals till constant weight. Germination essays were carried out with two treatments (control and scarification), with different seed MC (fresh, 10-12%, 3-5%) and in desiccated seeds (3-5% MC) stored six months at -20ºC. The MC in P. ferox seeds was 14.2% and 10% in P. nitens, for bothpopulations studied. Percentage germination in P. ferox was higher in the scarification treatments (&lt;82%). The difference between treatments increased with the reduction in MC and the storage for six months at -20°C. Fresh seeds of P. nitens do not need scarification treatment, but it is required with the reduction in MC and storage. Mean germination percentage of desiccated seeds stored six months at -20°C was similar in both populations and greater than 82%.We concluded that both species are probably orthodox because seeds tolerated desiccation to 3-5% and storage for six months at -20°C.","palabras_clave":"SEEDS|GERMINATION|MOISTURE CONTENT|NATIVE SPECIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63333,"idioma_id":4,"titulo":"Discrete axial motion of a radioactive tracer reconstructed from the response of axially aligned detectors: Application to the analysis of a bubble column dynamics","resumen":"The feasibility of extracting relevant dynamic information of a bubble column from the approximatereconstruction of a radioactive tracer axial trajectory using a set of axially aligned detectors (AAD) is explored. The experimental procedure involves scanning simultaneously different column heights with scintillation detectors, located vertically aligned beside the examined vessel, while a neutrally buoyant radioactive tracer particle is freely moving inside. The reconstruction considers that the detectors located closer in axial coordinate to the tracer are the ones which record the largest number of counts. Based on this assumption, time series of the approximate tracer axial coordinate are obtained with a maximum resolution of 2N&#8722;1 (N: number of detectors used). Compared to the powerful radioactive particle tracking (RPT) technique, although the information extracted is more limited, the present experimental procedure has the advantage of not requiring a calibration stage, which is rather cumbersome to implement at industrial scale and prevents RPT massive use for troubleshooting. Part of the information extracted from the analysis of tracer axial trajectories in RPT can also be inferred from the discrete tracer axial trajectories obtained with this methodology. Therefore, tracer fast axial velocity distributions, liquid axial mixing time and liquid holdup axial profiles are estimated from the reconstructed axial trajectories and related to the operating conditions, for experiments with water and non-Newtonian aqueous solutions of carboxymethylcellulose (CMC).","palabras_clave":"Axially Aligned Detectors|Radioactive tracers|Bubble column hydrodynamics|Mixing times|Gas holdup profiles|Flow regimes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63334,"idioma_id":4,"titulo":"Quantification of the water balance and hydrogeological processes of groundwater-lake interactions in the Pampa plain, Argentina","resumen":"This paper gives an account of the assessment and quantification of the water balance and the hydrogeological processes related to lake-groundwater interaction in the Pampa Plain by using hydrogeochemical, isotopic and flow numerical modeling techniques. La Salada is a permanent shallow lake, with an area of 5.8 km2, located on the SE of Buenos Aires Province. A total of 29 lake water samples and 15 groundwater samples were collected for both hydrochemical analysis and environmental stable isotope determination. Water table depths were measured in wells closed to the Lake.. Groundwater samples appear grouped on the Local Meteoric Water Line (LMWL), suggesting a well-mixed system and that rainfall is the main recharge source to the aquifer. Water evaporation process within La Salada is also corroborated by its isotopic composition. The model that best adjusts to La Salada Lake hydrochemical processes include: evaporation from groundwater, calcite precipitation with CO2 release and cationic exchange. The annual water balance terms for the lake basin indicates for each hydrological component the following values: 1.16 E08 m3 rainfall, 8.15 E07 m3 evapotranspiration, 1.90 E06 m3 runoff, 1.55 E07 m3 groundwater recharge, 6.01 E06 m3 groundwater discharge to the lake, 9.54 E06 m3 groundwater discharge to the river, 5.00 E05 m3 urban extraction and 4.90 E06 m3 lake evaporation. Integrated analysis of hydrochemical and isotopic information helped to calibrate the groundwater flow model, to validate the conceptual model and to quantitatively assess the basin water balance.","palabras_clave":"ENVIRONMENTAL ISOTOPES|HYDROGEOCHEMISTRY|FLOW MODELING|SHALLOW LAKE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63335,"idioma_id":4,"titulo":"The genomes of four tapeworm species reveal adaptations to parasitism","resumen":"Tapeworms (Cestoda) cause neglected diseases that can be fatal and are difficult to treat, owing to inefficient drugs. Here  we present an analysis of tapeworm genome sequences using the human-infective species Echinococcus multilocularis,  E. granulosus, Taenia solium and the laboratory model Hymenolepis microstoma as examples. The 115- to 141-megabase  genomes offer insights into the evolution of parasitism. Synteny is maintained with distantly related blood flukes but we  find extreme losses of genes and pathways that are ubiquitous in other animals, including 34 homeobox families and  several determinants of stem cell fate. Tapeworms have specialized detoxification pathways, metabolism that is finely  tuned to rely on nutrients scavenged from their hosts, and species-specific expansions of non-canonical heat shock  proteins and families of known antigens. We identify new potential drug targets, including some on which existing  pharmaceuticals may act. The genomes provide a rich resource to underpin the development of urgently needed  treatments and control.","palabras_clave":"Genome|tapeworms|parasitism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63336,"idioma_id":4,"titulo":"The nervous and prenervous roles of serotonin in Echinococcus spp.","resumen":"Serotonin (5-hydroxytryptamine, 5-HT) is an important neuroactive and morphogenetic molecule in several metazoan phyla, including flatworms. Serotoninergic nervous system studies are incomplete and 5-HT function\/s are unknown in Echinococcus spp., the flatworm parasites that cause hydatid disease. In the present work, we searched for genes of the serotoninergic pathway and performed immunocytochemical and functional analyses of 5-HT in Echinococcus spp. Bioinformatic analysis using the recently available Echinococcus multilocularis and Echinococcus granulosus genomes suggests the presence of genes encoding enzymes, receptors and transporters participating in 5-HT synthesis, sensing and transport in these parasites. However, some components of the pathway could not be identified, suggesting loss or divergence of parasite homologous genes. The serotoninergic neuroanatomy study performed by confocal scanning laser microscopy on different E. granulosus stages showed an increasing level of complexity when the protoscolex develops towards the adult stage and a progressive diminution when the parasite develops towards the metacestode stage. The role of 5-HT as a neurotransmitter in E. granulosus was evaluated by determining the effect of this substance on protoscolex motility. The addition of 5-HT to protoscoleces induced a significant increase in motility for short time periods. Preincubation with 100 lM citalopram, a known 5-HT transporter inhibitor, abolished the 5-HT-induced increase in motility, indicating that the effect could be mediated by a 5-HT transporter. Incubation of protoscoleces with 5-HT for time periods of several days induced a progressive differentiation towards the metacestode stage. The results indicate that 5-HT could have nervous and prenervous roles during Echinococcus spp. development. Taking into account the important roles of 5-HT in parasite biology and the divergence of 5-HT pathway genes with respect to human counterparts, the serotoninergic system could be considered as an amenable drug target against hydatid disease.","palabras_clave":"Echinococcus granulosus|Echinococcus multilocularis|Neurotransmitter|Protoscolex motility|Serotonin|Morphogen"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63337,"idioma_id":5,"titulo":"Bandas de sonido transnacionales: Voz e identidad en el cine brasileño contemporáneo","resumen":"A lo largo del siglo XX, en el marco de los cines nacionales, la textura del discurso y la sincronización de voces y cuerpos fueron centrales para la creación de lo que Benedith Anderson entendía por comunidad imaginada, al mismo tiempo que otros usos de la voz, en otras cinematografías, permiten articular sentidos que no necesariamente consolidan esas ideas de comunidad. Desde esta perspectiva, que considera la relación entre lo sonoro y el espacio, es posible problematizar qué pasa con las identidades nacionales en las películas del siglo XXI que se focalizan en desplazamientos trasnacionales.&nbsp; Teniendo en cuenta que la voz, en sincronía o diacronía con las imágenes exhibidas en pantalla, delimita espacios que sustentan o desestabilizan identidades culturales, en este artículo me gustaría reconsiderar la idea de una identidad territorial, lingüística y afectiva brasileña por medio del análisis de la lengua hablada y del uso de las voces en dos importantes filmes de la retomada: Terra Estrangeira, de Walter Salles y Daniela Thomas (1995), y Estorvo, de Ruy Guerra (2000). ","palabras_clave":"Cine brasileño|Desplazamientosq|Voz y acento|Afectividad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63338,"idioma_id":4,"titulo":"Sexual Differences in Life History Traits of Philornis seguyi  (Diptera: Muscidae) Parasitizing House Wrens (Troglodytes aedon).","resumen":"We studied life history traits of Philornis seguyi (Garcia, 1952) parasitizing house wrens, Troglodytes aedon, and analyzed sexual differences in the size of larvae, pupae and adults as well as in the length of larval and pupal stages and in adult survival. Males were larger than females at the larval, pupal and adult stages, but there were no sexual differences in the length of larval and pupal stages, or in the time adults survived. Larvae developed in 5-6 days and started the larval-pupal molt within 24 h after abandoning the host. Pupal stage lasted for 9 days and its length was positively associated with size of the pupa. The size of the pupa was positively associated with that of the larva and the size of the adult with that of the pupa. In laboratory conditions, 86% of the larvae pupated and 75% of the pupae emerged as adults. In natural conditions, the proportion of unsuccessful pupae increased by the end of host?s breeding season. The proportion of males and females that survived until the adult stage did not differ from random. Our results show that P. seguyi has a relatively short generation time, which would allow it a rapid population growth during the host breeding season and indicate that intrasexual selection may have selected for large body size in P. seguyi males.","palabras_clave":"Botfly parasitism|Sexual differences|Philornis seguyi|House Wren"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63339,"idioma_id":4,"titulo":"UV-B and abscisic acid effects on grape berry maturation and quality","resumen":"This review provides an overview of existing literature on the effects of ultraviolet-B (UV-B) radiation and abscisic acid (ABA) on physiological and biochemical aspects affecting grape berry (Vitis vinifera L.) growth, maturation and their quality for winemaking. The UV-B (280?315 nm) comprises only a small fraction of sunlight that reaches the Earth?s surface, but has enough energy to cause large photobiological effects on higher plants. The UV-B levels are greater in the tropics than in upper latitudes and also increase with altitude, hence some vineyards are exposed to high UV-B but photoprotection and repair mechanisms are usually sufficient to prevent the occurrence of damage in grapevine tissues. ABA is a phytohormone that, aside to control stomatal aperture, regulates acclimation to adverse environmental conditions, and controls grape berry maturation (non-climacteric). A promotive effect of UV-B on ABA biosynthesis has been found in grapevine leaves. Accumulation of phenols (namely phenolic acids, stilbenes and flavonoids) is an acclimation and protective response against UV-B, either directly by absorbing UV-B in epidermal tissues and\/or by reducing its penetration through underlying tissues, or indirectly by scavenging free radicals so acting as antioxidants. High UV-B and ABA applications increase total phenols in grape berries, but those with higher antioxidant capacity (i.e. dihydroxylated anthocyanidins and flavonols like quercetin) are increased relatively more. These treatments also hasten berry sugar and phenol accumulation, but reduce berry growth and sugar per berry at harvest, and therefore decrease yield. The quality of grape berries for winemaking integrates various aspects, but for red wines, it has a high correlation with accumulation of phenolics stimulated by UV-B and ABA.","palabras_clave":"UV-B radiation|abscisic acid|phenols|grapevines|berries|Vitis vinifera L."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63340,"idioma_id":4,"titulo":"Photophysics and photobiology of novel liposomal formulations of 2,9(10), 16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl]phthalocyaninatozinc(II)","resumen":"Six novel liposomal formulations of 2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl]phthalocyaninatozinc(II) (Pc9) were investigated to establish how the environments affect their photophysical and photobiological properties. The incorporation efficiency and solubility during a time period were evaluated. All Pc9-liposomal formulations were efficient singlet oxygen quantum yield generators. The photobiological potentials were evaluated on human nasopharynx KB carcinoma cells. None of the formulations studied showed cytotoxic effects in the absence of light, whereas all of them showed good performance after irradiation. The 50% inhibition of cell proliferation (IC50) was in the range of 0.21e0.47 mM for Pc9-loaded liposome formulations. A lysosomal localization was found for S-PEG as well as for S.","palabras_clave":"PHOTOPHYSICS AND PHOTOBIOLOGY|LIPOSOMAL FORMULATIONS|ZN PHTHALOCYANINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63341,"idioma_id":4,"titulo":"Photodynamic effects of  zinc(II) phthalocyanine-loaded  polymeric micelles in human nasopharynx KB carcinoma cells","resumen":"A major difficulty in photodynamic therapy is the poorsolubility of the photosensitizer (PS) under physiologicalconditions which correlates with low bioavailability. PSaggregation leads to a decrease in the photodynamic efficiencyand a more limited activity in vitro and in vivo. Toimprove the aqueous solubility and reduce the aggregation of2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl]phthal-ocyaninatozinc(II) (Pc9), the encapsulation into fourpoloxamine polymeric micelles (T304, T904, T1107 andT1307) displaying a broad spectrum of molecular weight andhydrophilic?lipophilic balance was investigated. The aqueoussolubility of Pc9 was increased up to 30 times. Morphologicalevaluation showed the formation of Pc9-loaded sphericalmicelles in the nanosize range. UV\/Vis and fluorescence studiesindicated that Pc9 is less aggregated upon encapsulationin comparison with Pc9 in water?DMSO 2% and remainedphotostable. Pc9-loaded micelles generated singlet molecularoxygen in high yields. Photocytotoxicity assays using humannasopharynx KB carcinoma cells confirmed that the encapsulationof Pc9 in T1107 and T1307 increases its photocytotoxicityby 10 times in comparison with the free form inwater?DMSO. In addition, Pc9 incorporated into cells wasmainly localized in lysosomes.","palabras_clave":"PHOTODYNAMIC EFFECTS|ZN PHTALOCYANINE|POLIMERIC MICELLES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63342,"idioma_id":4,"titulo":"Benthic diversity and assemblage structure of a north Patagonian rocky shore: a monitoring  legacy of the NaGISA project","resumen":"The rocky shore of Punta Este, Golfo Nuevo (Patagonia, Argentina), was sampled by means ofthe standardized NaGISA (CoML) protocol, that was aimed to generate biodiversity baselinedata in 6 levels, from high intertidal to 10m depth. Based on the generated data, we analyzed thebenthic assemblage structure, species richness, mean abundance and the distribution pattern ofinvertebrate functional groups, typifying species in each intertidal and subtidal level. Theintertidal sampled is exposed to extreme physical conditions higher than any other rocky shoresystem studied, with air temperature variation of 40°C during the year, maximum winds of 90km\/h and semidiurnal tides of 5m amplitude; on the other hand subtidal presents less thermalvariation (&#916;T 10°C along the year) and more homogeneous physical conditions. We identified64 taxa represented by six animal phyla: Mollusca, Arthropoda, Annelida (Polychaeta),Echinodermata, Cnidaria and Nemertea; and three algal phyla: Chlorophyta, Rhodophyta andHeterokontophyta (class: Phaeophyceae). Ordination nMDS plots showed three differentassemblages in terms of species composition (intertidal, subtidal 1m level and subtidal 5-10mlevels). The intertidal was represented by suspension feeders, mainly Mollusca. The tinymussels Brachidontes rodriguezii and Brachidontes purpuratus, and the algae Corallinaofficinalis dominated the intertidal and acts as a buffer that prevents other species for theextreme physical stress. The subtidal was mainly represented by grazers. Our results showed atendency of suspension feeders decreasing and increasing of grazers and predators from highintertidal to subtidal, probably driven by decreasing in physical stress. The gastropod Tegulapatagonica, the sea urchins Arbacia dufresnii and Pseudechinus magellanicus and the invasivealgae Undaria pinnatifida were the most abundant species in 1m-10m levels. Based on previousworks performed in the region, we hypothesize that the differences registered between intertidaland subtidal levels could be explained in part by an increase in physical stress at the intertidalwith low predation pressure that promotes positive interactions, while in the subtidal theincreasing in consumers and decreasing of physical stress could lead to associational defenses.Our results could be useful as baseline data to develop a sustainable network for long-termmonitoring benthic community changes due to anthropogenic activities.","palabras_clave":"BIODIVERSITY|ROCKY SHORES|PATAGONIA|PHYSICAL STRESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63343,"idioma_id":4,"titulo":"Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging application","resumen":"Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends upon complementation of two peptides, lac705&#945; and lac705&#946;) and bacteriocin\/s with strong anti-Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 °C was reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass adsorption maximum of 0.72 ± 0.05 &#956;g cm&#8722;2 and a minimum inhibition concentration of 1 &#956;g ml&#8722;1. The influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial bacteriocin\/s adsorption remained unaffected. To explain these results, a lack of lac705&#946; and lac705&#945; peptides complementation necessary for antimicrobial activity, while no interactions among impurities and antilisterial bacteriocin\/s during adsorption was suggested. Antilisterial bacteriocin\/s activity on the film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial bacteriocin\/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins while maintaining antilisterial antimicrobial activity. This study highlights the technological importance of adsorption optimization to obtain effective antimicrobial food packaging systems","palabras_clave":"BACTERIOCINS|ADSORPTION|ANTILISTERIA|ACTIVE PACKAGING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63344,"idioma_id":4,"titulo":"Granite emplacement by crustal boudinage: example of the Calmayo and El Hongo plutons (Córdoba, Argentina)","resumen":"This study deals with the structure and emplacement of the Calmayo and El Hongo trondhjemite plutons (Famatinian belt of C_ordoba, Argentina). It provides structural data from the granites and the country rocks and a study of the magnetic fabric in the plutons. New U\/Pb geochronological data yield intrusion ages of 512.1 _ 3.4 Ma and 500.6 _ 4.5 Ma for the Calmayo and El Hongo plutons respectively. The El Hongo massif and the southern part of the Calmayo trondhjemite preserve magmatic structures, whereas the northern domain of Calmayo shows the imprint of solid-state deformation. The main foliation in the country rocks outlines a boudin-like pattern at the map scale and the granites are located along boudin necks, suggesting that the emplacement of these trondhjemite plutons was linked to large-scale boudinage of the country rocks.                                                   ","palabras_clave":"emplacement|cortical boudinage|trondhjemite"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63345,"idioma_id":4,"titulo":"Photosensitized Generation of Singlet Oxygen from Re(I) Complexes: A Photophysical Study Using LIOAS and Luminescence Techniques","resumen":"Quantum yields and efficiencies of 1O2 (1&#916;g) production along with  photophysical properties for a number of Re(I) complexes in acetonitrile solutions are  reported. Two different classes of Re(I) complexes, LS-CO2-Re(CO)3(bpy) (LS = 2-  pyrazine, 2-naphthalene, 9-anthracene, 1-pyrene, 2-anthraquinone) and XRe(CO)3L (X =  CF3SO3, py; L = bpy, phen), were probed as photosensitizers for 1O2 (1&#916;g) production in  air-saturated acetonitrile solutions. Depending on the nature of the Re(I) complex, the  excited state responsible for the generation of 1O2 (1&#916;g) is either a metal-to-ligand charge  transfer (3MLCT) or a ligand centered (3LC) state. With LS-CO2-Re(CO)3(bpy)  complexes, 1O2 (1&#916;g) is produced by oxygen quenching of 3LC states of anthracene and  pyrene with high quantum yields (&#934;&#916; between 0.8 and 1.0), while the complexes bearing the ligands LS = 2-anthraquinone, 2-  pyrazine, and 2-naphthalene did not yield 1O2. XRe(CO)3L complexes generate 1O2 (1&#916;g) mainly by oxygen quenching of their  3MLCT luminescence with an efficiency of 1O2 (1&#916;g) formation close to unity. Bimolecular rate constants for the quenching of  the XRe(CO)3L complexes? emission by molecular oxygen range between 1 × 109 and 2 × 109 M&#8722;1 s&#8722;1, and they are all &#8804; 1\/9kd,  in good agreement with the predominance of the singlet channel in the mechanism of 1O2 (1&#916;g) generation using these Re(I)  complexes as photosensitizers. All the experimental singlet oxygen efficiencies are consistent with calorimetric and luminescence  data for the studied complexes. With LS-CO2-Re(CO)3(bpy) complexes, calorimetric experiments were utilized in the calculation  of the quantum yields of triplet formation; namely &#981;T = 0.76 and 0.83 for the triplet states of anthracene and pyrene, respectively.","palabras_clave":"PHOTOACUSTIC|RHENIUM|COMPLEXES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63346,"idioma_id":4,"titulo":"Biochemical responses and physiological status in the crab Hemigrapsus crenulatus (Crustacea, Varunidae) from high anthropogenically-impacted estuary (Lenga, south-central Chile)","resumen":"Estuarine environmental assessment by sub-individual responses is important in order to understand contaminant effects and to find suitable estuarine biomonitor species. Our study aimed to analyze oxidative stress responses, including glutathione-S-transferase (GST) activity, total antioxidant capacity (ACAP) and lipid peroxidation levels (TBARS) in estuarine crabs Hemigrapsus crenulatus from a high anthropogenically-impacted estuary (Lenga) compared to low and non-polluted estuaries (Tubul and Raqui), in a seasonal scale (winter\u2013summer), tissue specific (hepatopancreas and gills) and sex related responses. Results showed that hepatopancreas in male crabs better reflected inter-estuary differences. Morpho-condition traits as Cephalothorax hepatopancreas index (CHI) could be used as an indicator of physiological status of estuarine crabs. Discriminant analysis also showed that GST and TBARS levels in summer are more suitable endpoints for establishing differences between polluted and non-polluted sites. These results suggest the importance of seasonality, target tissue, sex and physiological status of brachyuran crabs for estuarine biomonitoring assessment.","palabras_clave":"GST|TBARS|HEPATOPANCREAS|HEMIGRAPSUS CRENULATUS|ESTUARY|MERCURY|PAHS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63347,"idioma_id":4,"titulo":"Biochemical and behavioral responses in the estuarine polychaete Perinereis gualpensis (Nereididae) after in situ exposure to polluted sediments","resumen":"In situ translocation experiments are advantageous relative to traditional laboratory experiments, particularly for understanding the bioavailability of trace metals like mercury (Hg). Individuals of the polychaete Perinereis gualpensis were translocated from a reference site (Raqui estuary, Chile) to an estuarine site with significant sediment Hg concentrations (Lenga estuary: 1.78\u20139.89 mg\/kg). Individuals were exposed in polluted and non-polluted sediments for 21 days and sampled every 7 days with cages deployed at three different depths. Tissue Hg concentrations were measured in conjunction with oxidative stress responses. Translocated polychaetes rapidly accumulated Hg. Glutathione S-transferase (GST) activities measured from posterior body regions were 2-fold higher than control activities after 21 days of exposure. Other antioxidant measures were idiosyncratic. Distinct burrowing behavior differences were observed; control polychaetes exhibited more homogenous vertical distributions, whereas in Lenga, worms tended to remain in upper layers. These studies demonstrate that under natural conditions, Hg is highly bioavailable to polychaetes affecting both biochemical and behavioral responses after relatively short-term exposure","palabras_clave":"IN SITU|MERCURY|GST|BEHAVIORAL RESPONSES|PERINEREIS GUALPENSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63348,"idioma_id":4,"titulo":"In silico structural and functional characterization of the RSUME splice variants","resumen":"RSUME (RWD-containing SUMO Enhancer) is a small protein that increases SUMO conjugation to proteins. To date, four splice variants that codify three RSUME isoforms have been described, which differ in their C-terminal end. Comparing the structure of the RSUME isoforms we found that, in addition to the previously described RWD domain in the N-terminal, all these RSUME variants also contain an intermediate domain. Only the longest RSUME isoform presents a C-terminal domain that is absent in the others. Given these differences, we used the shortest and longest RSUME variants for comparative studies. We found that the C-terminal domain is dispensable for the SUMO-conjugation enhancer properties of RSUME. We also demonstrate that these two RSUME variants are equally induced by hypoxia. The NF-&#954;B signaling pathway is inhibited and the HIF-1 pathway is increased more efficiently by the longest RSUME, by means of a greater physical interaction of RSUME267 with the target proteins. In addition, the mRNA and protein levels of these isoforms differ in human glioma samples; while the shortest RSUME isoform is expressed in all the tumors analyzed, the longest variant is expressed in most but not all of them. The results presented here show a degree of redundancy of the RSUME variants on the SUMO pathway. However, the increased inhibition conferred by RSUME267 over the NF-&#954;B signaling pathway, the increased activation over the HIF-1 pathway and the different expression of the RSUME isoforms suggest specific roles for each RSUME isoform which may be relevant in certain types of brain tumors that express RSUME, like human pituitary adenomas and gliomas.","palabras_clave":"Sumoylación|RSUME|Hipófisis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63349,"idioma_id":5,"titulo":"DIVERSIDAD DE MURCIÉLAGOS (MAMMALIA, CHIROPTERA) EN LA CIUDAD DE LULES, TUCUMÁN","resumen":"Se identificaron las especies de murciélagos y sus refugios en el área urbana de Lules, Tucumán. Es el primer estudio sistemático en Argentina en un área urbana. Se colectaron siete especies refugiadas principalmente en techos, entre las cuales es importante mencionar la captura de ejemplares de Eumops glaucinus, que representan el segundo registro para la provincia.Eumops glaucinus, que representan el segundo registro para la provincia.","palabras_clave":"Murcielagos|Refugios|Urbanos|Diversidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63350,"idioma_id":5,"titulo":"Los ectoparásitos de los roedores sigmodontinos (Cricetidae) de La Rioja: resultados preliminares","resumen":"El conocimiento sobre la fauna parasitaria es escasa o nula en muchas regiones de Argentina siendo La Rioja una de las provincias con menos registros, debido a los escasos muestreos de roedores sigmodontinos. El objetivo de este trabajo es reportar nuevos hospedadores y nuevas localidades geográficas para sifonápteros y ácaros parásitos de roedores sigmodontinos de la provincia de La Rioja. Las siguientes nuevas asociaciones fueron identificadas: Andalgalomys olrogi-Hectopsylla gracilis, Graomys chacoensis-Hectopsylla gracilis y Oligoryzomys cf. longicaudatus-Laelaps paulistanensis. Para la provincia se citan por primera vez Hectopsylla gracilis y las tres especies de ácaros registradas; y se da a conocer por primera vez un sifonáptero parásito para A. olrogi.","palabras_clave":"Pulgas|Acaros|La Rioja|Sigmodontinae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63351,"idioma_id":5,"titulo":"Pulgas (Siphonaptera) parásitas de roedores (Rodentia: Cricetidae) de la provincia de Salta, Argentina: nuevos registros de distribución","resumen":"Se mencionan nuevos registros de distribución geográfica y hospedatoria para las pulgas parásitas de roedores sigmodontinos de la provincia de Salta, Argentina. Son reportadas por primera vez para Salta las siguientes especies: Craneopsylla minerva minerva (Rothschild); Agatopsylla pearsoni Traub; y Neotyphloceras crassispina hemisus Jordan; asi como los géneros Cleopsylla  y Plocopsylla .  Agatopsylla pearsoni se cita por primera vez para el pais. Cinco especies de roedores incrementan su número de especies parasitas. Se registraron ocho nuevas asociaciones parasito-hospedador.","palabras_clave":"PULGAS|ROEDORES SIGMODONTINOS|ASOCIACION HOSPEDATORIA|DISTRIBUCION GEOGRAFICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63352,"idioma_id":4,"titulo":"Linking dynamical and functional properties of intrinsically bursting neurons","resumen":"Several studies have shown that bursting neurons can encode information   in the number of spikes per burst: As the stimulus varies, so does the   length of individual bursts.Therepresented stimuli, however, vary   substantially among different sensory modalities and different   neurons.The goal of this paper is to determine which kind of stimulus   features can be encoded in burst length, and how those features depend   on the mathematical properties of the underlying dynamical system.We   show that the initiation and termination of each burst is triggered by   specific stimulus features whose temporal characteristsics are   determined by the types of bifurcations that initiate and terminate   firing in each burst. As only a few bifurcations are possible, only a   restricted number of encoded features exists. Here we focus specifically   on describing parabolic, square-wave and elliptic bursters. We find   that parabolic bursters, whose firing is initiated and terminated by   saddle-node bifurcations, behave as prototypical integrators: Firing is   triggered by depolarizing stimuli, and lasts for as long as excitation   is prolonged. Elliptic bursters, contrastingly, constitute prototypical   resonators, since both the initiating and terminating bifurcations   possess well-defined oscillation time scales. Firing is therefore   triggered by stimulus stretches of matching frequency and terminated by a   phase-inversion in the oscillation. The behavior of square-wave   bursters is somewhat intermediate, since they are triggered by a fold   bifurcation of cycles of well-defined frequency but are terminated by a   homoclinic bifurcation lacking an oscillating time scale. These   correspondences show that stimulus selectivity is determined by the type   of bifurcations. By testing several neuron models, we also demonstrate   that additional biological properties that do not modify the bifurcation   structure play a minor role in stimulus encoding. Moreover, we show   that burst-length variability (and thereby, the capacity to transmit   information) depends on a trade-off between the variance of the external   signal driving the cell and the strength of the slow internal currents   modulating bursts. Thus, our work explicitly links the computational   properties of bursting neurons to the mathematical properties of the   underlying dynamical systems.","palabras_clave":"BURSTS|NEURAL CODING|DYNAMICAL SYSTEMS|COVARIANCE ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63353,"idioma_id":4,"titulo":"First report of microcystins and anatoxin-a co-occurrence in San Roque reservoir (Córdoba-Argentina)","resumen":"The aim of this study was to evaluate the presence of microcystin-LR, microcystin-RR,microcystin-YR, and the neurotoxin anatoxin-a in water samples collected monthly during 1 year inSan Roque reservoir (Córdoba, Argentina) to identify the environmental factors that could&nbsp; promote the presence of these cyanotoxins. The HPLC-UV and MS\/MS analysis showed the presence of microcystin in most of the sampling times, even when Cyanobacteria were subdominant. Microcystin concentrations varied from not detectable levels to 119.0 &#956;g\/ L. Thus, they frequently surpassed the guidelines suggested by WHO for drinking water (1 &#956;g\/ L) and recreational exposure (20 &#956;g\/ L). To the extent of our knowledge, this is the first report ofanatoxin-a in freshwaters in South America. Anatoxin-a concentrations varied from not detectable levels to 6.6 ng\/ L, a thousand times below the provisional guideline adopted by New Zealand for drinking water. Microcystin showed significant correlation with Microcystis and Pseudoanabaena while anatoxin-a correlated with Oscillatoria and Anabaena counts. Linear discriminant analysis showed that higher pH levels and more variable chlorophyll-a concentrations were measuredin San Roque reservoir when cyanotoxins were present. Lower inorganic nitrogen concentrations were observed in autumn, when the prevalence of Anabaena became significant in Cyanobacteria composition and highest anatoxin-a levels were measured. The observed dynamic of phytoplankton going together with the cyanotoxins occurrence could be explained by the hypothesis of cyanotoxins acting as allelopathic compounds.The microcystin levels measured plus the presence of anatoxin-a show the need of stronger management efforts to preserve human and wildlife health.","palabras_clave":"Cyanotoxins|HPLC-MS\/MS|Neurotoxins|Hepatotoxins|Environmental factors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63354,"idioma_id":4,"titulo":"Asymmetrical behavioral response towards two boron toxicants depends on the ant species (Hymenoptera: Formicidae)","resumen":"Urban ants are a worldwide critical household pests, and efforts to control themusually involve the use of alimentary baits containing slow-acting insecticides. A common toxicant used is boron, either as borax or boric acid. However, the presence of these compounds can affect the consumption of baits by reducing their acceptance and ingestion. Moreover, as feeding motivation varies widely, according not only to food properties but also to colony conditions, bait consumption might be diminished further in certain situations. In this study, we compared the feeding response of ants toward two boron toxic baits (boric acid and borax) in low motivation situations that enhance any possible phago-deterrence the baits may produce. Most studies investigating bait ingestion evaluate whole nests or groups of ants; here, we analyzed the individual ingestion behavior and mortality of the Argentine ant, Linepithema humile (Mayr), and the carpenter ant, Camponotus mus (Roger), for two boron baits, to detect which compound generates a higher rejection in each of these species. Although these two species have similar feeding habits, our results showed that ants under lowmotivation conditions reduced the acceptance and consumption of the toxic baits asymmetrically. While L. humile mostly rejected the borax, C. mus rejected the boric acid. These results denote the importance of considering the preference of each species when developing a pest management strategy.","palabras_clave":"ANT|BORIC ACID|SODIUM BORATE|FEEDING|BAIT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63355,"idioma_id":4,"titulo":"Genetic structure of Argentinean hexaploid wheat  germplasm","resumen":"The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely  linked to genes and neutral) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified, which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.","palabras_clave":"Wheat|Genetic structure|Molecular Markers|Genetic Diversity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63356,"idioma_id":4,"titulo":"Odour Profile of Beef using an Electronic Nose Based on MOS-Sensor","resumen":"The  development of the aroma and flavour of cooked meat is a very complex process in  which different components react to produce chemical intermediates or final  flavour volatiles compounds. The aim of the present research was to study the  odour profile of beef produced under different feeding regimes and cooking  conditions using an electronic nose based on MOS sensors to monitor the odour  characteristics. Odour profiles of different grilled-cooked muscles were  evaluated. A clear discrimination between groups corresponding to muscle was  observed. Grilled-cooked samples of Longissimus dorsi muscle from animals  fed to other cooking process. under different diets showed a clear  discrimination between groups. Finally, Striploins samples cooked by moist-heat  method tend to be different when compared.","palabras_clave":"Electronic nose| Volatile compounds|Meat| MOS-sensors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63358,"idioma_id":4,"titulo":"The effect of pre-slaughter stressors on physiological indicators and meat quality traits on Merino lambs","resumen":"Merino lambs of 90 days of mean age (standard deviation -s.d.- 6 days) and 22.0 Kg of mean live weight (s.d. 2.7 kg) were used to explore the effects of pre-slaughter stressors on physiological characteristics and meat quality attributes. Three stressors were studied in a controlled experiment: Fasting (food deprivation for 24 hours before slaughter), physical Exercise (keeping animals walking for 30 minutes at approximately 3 km\/h) and Fear stress (exposing animals to barking dogs for 5 minutes). A fourth treatment was kept as a Control. Fasted lambs had greater (P  0.05) in physiological indicators and lambs exposed to barking dogs had greater (P < 0.05) cortisol concentration compared with Control. The stressor treatments studied did not affect meat quality parameters. Therefore, even though the stressors imposed on the lambs induced changes in blood constituents typically associated with the stress response, the intensity and (or) duration of these stressors had no effect on meat quality traits.","palabras_clave":"lamb|meat quality|pre slaughter|stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63360,"idioma_id":4,"titulo":"Maritrema orensense and Maritrema bonaerense (Digenea: Microphallidae): descriptions, life cycles, and comparative morphometric analyses","resumen":"We elucidate the life cycle of Maritrema orensense for the first time and experimentally confirm that of the sympatric Maritrema bonaerense. In Argentinean estuaries, both species parasitize the cochliopid snail Heleobia australis as first intermediate host, the grapsid crabs Neohelice granulata and Cyrtograpsus angulatus as second intermediate hosts, and gulls as definitive hosts. We describe the daughter sporocysts and cercariae of M. orensense, and redescribe these stages for M. bonaerense. Sporocysts of M. orensense were shorter, with fewer developed cercariae than M. bonaerense. The cercaria of M. orensense had longer, bigger, and more undulating penetration glands than M. bonaerense. We redescribe metacercariae and adults of both species, and compare them with the previous descriptions. Intestinal cecum length, vitellaria shape and extension, and egg size are the most relevant characteristics in metacercariae and adults for differentiating the species. Hence, the detailed morphological description and comparative analyses of morphometrics obtained from natural and experimental infections permit clear differentiation of M. orensense and M. bonaerense at each life stage.","palabras_clave":"EXPERIMENTAL INFECTION|PARASITE|COCHLIOPID SNAIL|GRAPSID CRAB"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63361,"idioma_id":5,"titulo":"Digeneos parásitos de juveniles de Mugil liza (Pisces: Mugilidae) en la Bahía de Samborombón, Argentina, con el reporte de  metacercarias zoonoticas de Ascocotyle (Phagicola) longa.","resumen":"El presente trabajo representa el primer estudio sobre digeneos, estadios larvales y adultos parásitos de juveniles de la lisa, Mugil liza Valenciennes, 1836 de las áreas de cría de la Bahía de Samborombón, Argentina. En el intestino se ha encontrado Dicrogaster fastigata Thatcher y Sparks, 1958, Hymenocotta manteri Overstreet, 1969, en la cavidad abdominal Phyllodistomum mugilis Knoff y Amato, 1992, en la musculatura y los mesenterios la metacercaria de Ascocotyle (Phagicola) longa Ransom, 1920 causante de heterofasiasis en el ser humano y en la cavidad abdominal una metacercaria desenquistada de la familia Hemiuridae. Este trabajo representa una nueva cita para Argentina de H. manteri y P.mugilis, a su vez para este último se amplia el rango de distribución, representanado la segunda cita a nivel mundial.","palabras_clave":"MUGIL LIZA|DIGENEOS|PARASITOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63363,"idioma_id":4,"titulo":"Morphological aspects and histological effects of the attachment organ of Parabrachiella sp. (Copepoda: Lernaeopodidae) on the grey mullet, Mugil liza Valenciennes","resumen":"The genus Parabrachiella Wilson, 1915 (Lernaeopodidae)  is represented by copepods that are  highly adapted to a parasitic way of life. In Argentina,  only P. insidiosa var. lageniformis Heller,  1865, P. chevreuxii Van Beneden, 1891 and  P. spinicephala Ringuelet, 1945 have been cited,  but none of these have been reported on mugilids.  Recently, other species of this genus were found  attached to the nasal cavities of juvenile grey mullets,  Mugil liza Valenciennes, from Samborombon  bay, Buenos Aires province. In this study, the  prevalence and mean intensity of the Parabrachiella  sp. on grey mullet is investigated. In addition,  the damage the parasite imposes on its hosts is  examined through evaluation of histological sections  and immunostaining for proliferative cell  nuclear antigen (PCNA). The morphology of the  parasite\u2019s bulla is described from light and scanning  electron micrographs.","palabras_clave":"Copepoda|Parabrachiella|PCNA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63364,"idioma_id":4,"titulo":"A new ornithopod (Dinosauria; Ornithischia) from Antarctica","resumen":"A new ornithopod dinosaur from Antarctica, Trinisaura santamartaensis n. gen. et n. sp. is diagnosed by a unique combination of characters that includes a scapula with a spike-like acromial process with a strong and sharp lateral crest and longer than other ornithopods, a humerus with a rudimentary deltopectoral crest represented as a thickening on the anterolateral margin of the humerus, and shaft strongly bowed laterally, and an ischium gently curved along its entire length. The holotype specimen comprises vertebral and appendicular elements. The presence of axially elongate distal caudal vertebrae, pubis with long prepubic and postpubic processes, as well as a femur with a distinct anterior trochanter, pendant 4th trochanter and shallow anterior intercondylar groove constitute a combination of characters present in the Late Cretaceous Patagonian Gasparinisaura, Anabisetia and Talenkahuen. The materials were found on the surface enclosed in a hard sandstone concretion collected near the Santa Marta Cove, James Ross Island, from the lower levels of the Snow Hill Island Formation (Campanian). This is the first ornithopod taxon identified from this unit, and the second ornithischian dinosaur, after the ankylosaur Antarctopelta oliveroi. However, other ornithopod reports from nearby localities of James Ross and Vegaislands in outcrops of the overlying Lopez de Bertodano Formation suggest that this clade was widely represented in the Campanian and Maastrichtian of the James Ross Basin, Antarctic continent.","palabras_clave":"Dinosauria|Maastrichtian|Antarctica|Ornithopoda"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63365,"idioma_id":4,"titulo":"Main pathways in the evolution of the Paleogene Antarctic Sphenisciformes","resumen":"The Seymour Island fossil penguins from the Cross Valley and La Meseta Formations, Seymour Island (James Ross Basin; late Paleocene, and Eocene\/?early Oligocene respectively) constitute the most com-  plete stratigraphic records of the group known in the world. In addition to the geographic and stratigraphic thoroughness of the collected fossil remains, they offer a unique opportunity to the  understanding of the major evolutionary patterns of the Sphenisciformes. We analyze their taxonomic diversity and abundance in La Meseta Formation, in a context of the spread patterns of the Paleogene  penguins in relationship to the Antarctic Circumpolar Current incidence. Our data suggest that the highest diversity and geographical distribution (Antarctica, South America and Australasia) in the Late  Eocene were linked to water-cooling events and the opening of the Drake Passage. However, the first divergence of the group is documented in the Early Eocene. This fact supports that a marine dispersion of  the Weddellian penguins to other continents occurs when there was a shallow and warm ocean current circulation (Weddellian Current) flowing along the western margin of West Antarctica and South  America The phylogenetic analysis and the recent discoveries in the Eocene of Chile and Peru support the hypothesis that establishes one of the way for the dispersion of the Weddellian species from the  Peninsula Antarctica was the Pacific coast during the early Eocene.","palabras_clave":"SPHENISCIFORMES|BIODIVERSITY|ANTARCTICA|WEDDELLIAN CURRENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63366,"idioma_id":5,"titulo":"Presencia de zearalenona  en pasturas del este de Chaco","resumen":"Se estudió la presencia de zearalenona en pasturas y alimentos que son utilizados para la producción debovinos de carne de la zona este de la provincia del Chaco. Asimismo, se realizó el aislamiento e identificaciónde diversas especies de Fusarium y se determinó su capacidad de producir zearalenona en condicionesde laboratorio. La determinación química de la toxina se realizó mediante las metodologías HPLC y ensayoinmunoenzimático (ELISA). Se analizaron 29 muestras y en 17 de ellas se confirmó la ocurrencia natural dezearalenona en cantidades variables. Se identificaron 63 aislamientos que correspondieron a Fusarium cf.acuminatum, F. chlamydosporum, F. equiseti, F. oxysporum y F. semitectum, confirmándose además la producciónin vitro de zearalenona en 34 de éstos aislamientos.","palabras_clave":"Micotoxinas|Fusarium|Pastos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63367,"idioma_id":4,"titulo":"The opening of the Magellan Strait and its geodynamic implications","resumen":"The existence of a Neogene-Quaternary rift system responsible for the opening of the eastern Magellan strait has been one of the more peculiar tectonic features of the southernmost Andes since its discovery more than a decade ago. However, until now there has been no explanation pinpointing the specific tectonic factors that led to the development of the rift. This work focuses on the interaction between the fault systems and the tectonic regimes that have affected Tierra del Fuego north of the South America?Scotia left lateral strike-slip plate boundary. Due to a good match between a Jurassic extensional fault array orientation and the principal stress directions related to the strike-slip plate boundary, we conclude that Jurassic transfer faults reactivated extensionally generating the Magellan rift system. This process was enhanced by northward continental drift, and has implications for the geodynamic behind the separation of South America from Antarctica.","palabras_clave":"MAGELLAN STRAIT|DRAKE PASSAGE|SOUTHERNMOST ANDES|TECTONIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63368,"idioma_id":4,"titulo":"Phenological variations of polyphenols in Smilax campestris (Smilacaceae)","resumen":"Polyphenol profiles can suffer quali-quantitative modifications as the plant modifies its phenological condition. The objectiveof this work was to determine if there is a rhythm of production in the synthesis of polyphenols according to the phenological conditionin the leaves, roots, and rhizomes of Smilax campestris Griseb. The plant material analysed corresponded to individuals of a colonyof S. campestris collected in different phenological conditions. Standard methodology was used for the chromatographic profiles offlavonols (kaempferol, quercetin, isorhamnetin, and their glycosides) and proanthocyanidins (procyanidin and propelargonidin) andquantification of total phenol, condensed tannins, and flavonols. Appearance of metabolic changes was established in the studied organsof S. campestris according to the phenological condition.Smilax campestris Griseb. The plant material analysed corresponded to individuals of a colonyof S. campestris collected in different phenological conditions. Standard methodology was used for the chromatographic profiles offlavonols (kaempferol, quercetin, isorhamnetin, and their glycosides) and proanthocyanidins (procyanidin and propelargonidin) andquantification of total phenol, condensed tannins, and flavonols. Appearance of metabolic changes was established in the studied organsof S. campestris according to the phenological condition.S. campestris collected in different phenological conditions. Standard methodology was used for the chromatographic profiles offlavonols (kaempferol, quercetin, isorhamnetin, and their glycosides) and proanthocyanidins (procyanidin and propelargonidin) andquantification of total phenol, condensed tannins, and flavonols. Appearance of metabolic changes was established in the studied organsof S. campestris according to the phenological condition.S. campestris according to the phenological condition.","palabras_clave":"SMILAX CAMPESTRIS|PHENOLOGICAL  CONDITION|POLIPHENOLS|FLAVONOIDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63369,"idioma_id":4,"titulo":"Understanding grain yield responses to source-sink ratios during grain filling in wheat and barley under contrasting environments","resumen":"A better understanding of the effects of different source?sink ratio during the grain filling period on grain growth may be relevant in order to further increase cereal grain yield. The main objective of the present work was to determine the effect of different manipulations of the source?sink ratios in wheat and barley grown at four different environmental conditions on responsiveness of sinks (grain growth and yield) and sources (spike photosynthesis and water soluble carbohydrates in the stems). Four treatments were imposed 7 days after anthesis in two contrasting locations with low- and high-inputs conditions in wheat (cv. Soissons) and barley (cv. Sunrise): they were a control, a treatment removing all the spikelets from the upper half of the spikes (TS), and shadings decreasing incident radiation by 75% on the whole canopy (SW) or only on the leaves (having the top area of the meshes individual holes for each spike to be exposed to solar radiation, SL). As expected grain yield was closely related to grain number per m2. Average grain weight was reduced by shading treatments far more markedly in Sw than in SL. Interestingly, significant amounts of water soluble carbohydrates in the stems remained at maturity in SL and Sw treatments and spike photosynthesis in SL was consistently higher than in the unshaded controls in both species. These results may be an indication that wheat and barley are not source-limited during grain filling and that only when subjected to an extremely severe stress, grain size would be reduced due to lack of enough assimilates available to fill them.","palabras_clave":"Grain number|Grain weight|Spike photosynthesis|Stem reserves"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63370,"idioma_id":5,"titulo":"Dinámica de polifenoles y estudio anatomo-histoquímico en Schinus longifolius (Lindl.) Speg. (Anacardiaceae) en respuesta a la infección por Calophya mammifex (Hemiptera \u2013 Calophyidae)","resumen":"Se analizaron los fenoles totales, taninos totales, proantocianidinas, flavonoides totales y ácidos hidroxicinámicos totales de hojas sanas y agallas de Schinus longifolius (Lindl.) Speg. (Anacardiaceae) infectado por Calophya mammifex (Hemiptera - Calophyidae). La agalla presenta una variación cuali-cuantitativa de los polifenoles estudiados, con una disminución de los fenoles, taninos, flavonoides y ácidos hidroxicinámicos y un aumento en el contenido de proantocianidinas, cuyo monómero se transforma en cianidina luego del clivaje ácido. Se realizó además un análisis anatómico de la agalla donde se encontraron acúmulos de esclereidas y un mayor desarrollo vascular respecto de la hoja sana y un ensayo histoquímico que mostró una expresión diferencial de metabolitos polifenólicos en sus tejidos. C. mammifex utiliza la agalla como refugio contra las condiciones climáticas adversas y predadores y provee además de alimento con menos compuestos potencialmente perjudiciales en las etapas tempranas de su desarrollo.","palabras_clave":"Agallas|Calophya mammifex|Polifenoles|Schinus longifolius"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63371,"idioma_id":4,"titulo":"The importance of substrate size and interstitial space in the microhabitat selection by the stream-dwelling catfish Hatcheria macraei (Actinopterygii, Trichomycteridae )","resumen":"The study tests whether diurnal microhabitat use by Hatcheria macraei depends upon specific environmental parameters and\/or the abundance of other fish. We carried out a 1-year field study in a low order river of northern Patagonia, Pichileufu River,and used experimental trials to  determine substrate preferences. Fishes were captured during daylight andphysicochemical environmental variables were recorded. Headwater zones were dominated by rainbow trout (Oncorhynchus mykiss) and brown trout(Salmo trutta), while native fishes, H. macraei and the creole perch (Percichthys trucha) were more abundant downstream. H. macraei inhabited mostly shallow microhabitats with fast water velocity and substrateshaving significant interstitial spaces, independently of the abundance of other fishes. Experimental trials pointed out that H. macraei preferred mostly coarser substrates ([6 cm), avoiding fine ones. This study highlights the importance of erosional zones with high water velocity, large substrates, and suitable interstitial space in the microhabitat selection of H. macraei. The knowledge of microhabitat use by native fishpopulations is critical for management and conservation strategies and should be taken into account before any river modification","palabras_clave":"Trichomycteridae |Habitat selection |Fish assemblage variation|Stream substrate preference|Fish density"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63372,"idioma_id":4,"titulo":"The genus Gymnotus (Gymnotiformes: Gymnotidae) in Argentina. How bad taxonomy results in poor regulations and no conservation","resumen":"Four species of the genus Gymnotus are present in Argentina:  G. inaequilabiatus, G. omarorum, G. pantanal, and G. sylvius,  the last three species being recorded for the first time in  freshwater courses. Gymnotus omarorum, G. pantanal, and  G. sylvius together with others of the genus Brachyhypopomus  are the group of fishes that bear the greatest impact in  the trade as live bait for sport fishing in the northeastern  region of Argentina. Within this large area, only the provinces  of Chaco, Corrientes, and Formosa have regulations  for the catch, trade, and sale of species as live bait. Unfortunately,  the species covered by legal regulations are Gymnotus  carapo and Brachyhypopomus brevirostris, neither of which  occurs in freshwater habitats of Argentina. Comments are  included as to how this bad taxonomy affects the regulations  and conservation status of these species.","palabras_clave":"GYMNOTUS|CONSERVATION|FISHES|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63373,"idioma_id":4,"titulo":"Cytogenetics of the Porthole Shovelnose Catfish, Hemisorubim platyrhynchos (Valenciennes, 1840) (Siluriformes, Pimelodidae), a widespread species in South American rivers","resumen":"Hemisorubim platyrhynchos is a medium- to large-sized pimelodid catfish distributed along several river  basins of the Neotropical Region, noteworthy for representing an important fishery source. In this work,  H. platyrhynchos from three isolated populations were cytogenetically analyzed. The karyotype shows a  diploid number of 2n=56 chromosomes comprising 22m, 16sm, 10st, 8a (FN=104). NORs detected by  AgNO3 were located in the terminal regions of the short arm of a st chromosome pair, as confirmed by  CMA3 and FISH using an 18S rDNA probe. C-banding revealed a small amount of heterochromatin in  chromosomes, including the NORs, and one biarmed pair that showed conspicuous positive bands on  both arms. This fact was also evidenced when using other banding techniques, such as RE (AluI), and  indicates that this pair constitutes a species-specific cytogenetic marker.","palabras_clave":"HEMISORUBIM|FISHES|CYTOGENETICS|CATFISH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63374,"idioma_id":4,"titulo":"Effect of high molecular weight glutenins and rye translocations on soft wheat flour cookie quality","resumen":"The influence of high molecular weight glutenin subunits (HMW-GS) on wheat breadmaking quality has been extensively studied but the effect of different Glu-1 alleles on cookie quality is still poorly understood.  This study was conducted to analyze the effect of HMW-GS composition and wheat-rye translocations on physicochemical flour properties and cookie quality of soft wheat flours. Alleles encoded at Glu-A1, Glu-B1 and Glu-D1 locus had a significant effect over physicochemical flour properties and solvent  retention capacity (SRC) profile. The null allele for Glu-A1 locus presented the highest cookie factor observed (CF ¼ 7.10), whereas 1BL\/1RS and 1AL\/1RS rye translocations had a negative influence on CF. The three cultivars that showed the highest CF (19, 44 and 47) had the following combination: Glu-A1 ¼ null, Glu-B1 ¼7 þ 8, Glu-D1 ¼ 2 þ 12 and no secalins. Two prediction equations were developed to estimate soft wheat CF: one using the HMW-GS composition and the other using physicochemical flour parameters, where SRCsuc, SRC carb, water-soluble pentosans, damaged starch and protein turned out to be better CF predictors. This data suggests that grain protein allelic composition and physicochemical flour properties can be useful tools in breeding programs to select soft wheat of good cookie making quality.","palabras_clave":"Soft wheat | High molecular weight glutenins|Wheat-rye translocation |Cookie"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63375,"idioma_id":4,"titulo":"Cellulose recycling as a source of raw chirality","resumen":"  Modern organic chemistry requires easily obtainable chiral building blocks that show high chemical versatility for their application in the synthesis of enantiopure compounds. Biomass has been demonstrated to be a widely available raw material that represents the only abundant source of renewable organic carbon. Through the pyrolitic conversion of cellulose or cellulose-containing materials it is possible to produce levoglucosenone, a highly functionalized chiral structure. This compound has been innovatively used as a template for the synthesis of key intermediates of biologically active products and for the preparation of chiral auxiliaries, catalysts, and organocatalysts for their application in asymmetric synthesis.","palabras_clave":"ASYMMETRIC SYNTHESIS|BIOMASS|CATALYSIS|CELLULOSE|CHIRAL AUXILIARIES|D-ALLAL|LEVOGLUCOSENONE|ORGANOCATALYSTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63376,"idioma_id":5,"titulo":"' Buscando paz. Peregrinos al cerro de las  Apariciones de la  Virgen  en Salta'","resumen":"En Salta, al norte de la Argentina, desde el 2001 todos los sábado se congregan miles de personas que acompañan a una mujer, M. Livia, que afirma ver y recibir mensajes de la Virgen. El fenómeno se ha ido consolidando a lo largo de los años pese al creciente distanciamiento por parte de la Iglesia local. Lo que sucede en Salta y el evento que actualmente tiene lugar en San Nicolás - Pcia. de Buenos Aires-, son los dos fenómenos de \u201CApariciones de la Virgen\u201D que mayor cantidad de gente congregan y de mayor notoriedad pública.  Nuestra  preocupación por conocer el fenómeno nos ha llevado a abordarlo desde aproximaciones cualitativas y cuantitativas. En este  artículo presentamos los resultados de un abordaje cuantitativo realizado   a través de una encuesta  a peregrinos. Nos interesaba abordar  aspectos vinculados a las motivaciones para participar,  a  sus vivencias durante la participación en la ceremonia junto a la vidente, y al conocimiento y percepción que tienen del fenómeno. Preguntamos asimismo aspectos vinculados a su situación sociodemográfica, creencias, identidad y práctica religiosa. Estos últimos los comparamos con los datos provenientes de la Primera Encuesta Argentina sobre Religión y Estructura social efectuada en el 2007 por el equipo coordinado por el Dr. Mallimaci. Esta comparación nos permitió avanzar en el conocimiento la especificidad sociorreligiosa de las personas que concurren al lugar.","palabras_clave":"APARICIONES MARIANAS|DEVOCIONES MARIANAS|SALTA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63377,"idioma_id":4,"titulo":"Chemical  and  functional  properties  of  cell  wall  polymers  from  two  cherry varieties  at  two  developmental  stages","resumen":"The cell wall polysaccharides of Regina and Sunburst cherry varieties at two developmental stages were extracted sequentially, and their changes in monosaccharide composition and functional properties were studied. The loosely-attached pectins presented a lower d-galacturonic acid\/rhamnose ratio than ionically-bound pectins, as well as lower thickening effects of their respective 2% aqueous solution: the lowest Newtonian viscosity and shear rate dependence during the pseudoplastic phase. The main constituents of the cell wall matrix were covalently bound pectins probably through diferulate cross-linkings), with long arabinan side chains at the RG-I cores. This pectin domain was also anchored into the XG-cellulose elastic network. Ripening occurred with a decrease in the proportion of HGs, water extractable GGM and xylogalacturonan, and with a concomitant increase in neutral sugars. Ripening was also associated with higher viscosities and thickening effects, and to larger distribution of molecular weights. The highest firmness and compactness of Regina cherry may be associated with its higher proportion of calcium-bound HGs localized in the middle lamellae of cell walls, as well as to some higher molar proportion of NS (Rha and Ara) in covalently bound pectins. These pectins showed significantly better hydration properties than hemicellulose and cellulose network. Chemical composition and  functional properties of cell wall polymers were dependent on cherry variety and ripening stage, and helped explain the contrasting firmness of Regina and Sunburst varieties.","palabras_clave":"SWEET CHERRY|CELL WALL BIOPOLYMERS|POLYSACCHARIDES|RIPENING|FUNCTIONAL PROPERTIES|FIRMNESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63378,"idioma_id":4,"titulo":"Blanching of red beet (Beta vulgaris L. var. conditiva) root. Effect of hot water or microwave radiation on cell wall characteristics","resumen":"The effect of enzymes inactivation through traditional (water, 90 C) and microwave blanching (constant power of 350Wand changing power with values higher than 900 W) on cell wall polysaccharides as well as on microstructure of beet root tissue was studied with the goal of attaining a better understanding of the changes produced by treatment and its consequences on the functional properties of cell wall polymers. Powers higher than 900 W produced greater tissue microstructural damage due to alteration of the cell wall network. The contact between neighboring cells persisted after microwave treatment at 350 W while traditional blanching or blanching at higher microwave powers (90MW) produced separation of the middle lamella at different points and also cell separation was observed for the last mentioned treatment. The formation of entanglements of pectin-in-extensin in 350 W-treated beet root and the higher content of calcium and diferulated cross linked pectins in the case of traditional treatment may account for the better mechanical performance observed for these tissues. The microwaving at 350 W modified the cell wall polymers in such a manner that produced an increase in their hydrophilicity.","palabras_clave":"remolacha|escaldado|microondas|pared celular y microestructura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63379,"idioma_id":4,"titulo":"First record of gastroliths associated with elasmosaur remains from La Colonia Formation (CampanianeMaastrichtian), Chubut, Patagonia Argentina, with comments on the probable depositional palaeoenvironment of the source of the gastroliths","resumen":"A gastrolith set, comprising 197 elements, associated with an indeterminate elasmosaurid plesiosaur collected from the CampanianeMaastrichtian La Colonia Formation, Chubut, Argentina, is described. Most of the gastroliths are discoidal (41.9%) or spheroidal (34.8%), with a mean Maximum Projection Sphericity Index value of 0.69 and a mean OP Index value of 0.74. The values of these indices are compared with those recorded for gastroliths associated with other Upper Cretaceous elasmosaurids to see if patterns with palaeobiological relevance are evident. The mean values of the Maximum Projection Sphericity Index and the Oblate-Prolate Index allow us to infer a fluvial or estuarine origin for all the elasmosaurid gastroliths for which these indices have been calculated. This inference is palaeobiologically informative because it indicates that at least some Upper Cretaceous elasmosaurs entered into estuarine (or fluvial) environments. It also helps explain the interesting vertebrate assemblage of the Allen and La Colonia formations where a rich continental fauna is recorded but elasmosaurids and polycotylids are the only well represented vertebrates with marine affinities.","palabras_clave":"Gastroliths|Plesiosaurs|La Colonia Formation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63380,"idioma_id":4,"titulo":"The Behavioral Implications of a Multi-Individual Bonebed of a Small Theropod Dinosaur","resumen":"Background: Central Patagonia, Argentina, preserves an abundant and rich fossil record. Among vertebrate fossils from the Upper Cretaceous Bajo Barreal Formation of Patagonia, five individuals of the small, non-avian theropod dinosaur Aniksosaurus darwini were recovered. Group behavior is an important aspect of dinosaur paleoecology, but it is not well-documented and is poorly understood among non-avian Theropoda. Methods\/Principal Findings: The taphonomic association of individuals from the Bajo Barreal Formation and aspects of their bone histology suggest gregarious behavior for Aniksosaurus, during at least a portion of the life history of this species. Histology indicates that the specimens were juvenile to sub-adult individuals. In addition, morphological differences between individuals, particularly proportions of the appendicular bones, are probably related to body-size dimorphism rather than ontogenetic stage. Conclusions\/Significance: Gregarious behaviour may have conferred a selective advantage on Aniksosaurus individuals, contributing to their successful exploitation of the Cretaceous paleoenvironment preserved in the Bajo Barreal Formation. The monospecific assemblage of Aniksosaurus specimens constitutes only the second body fossil association of small, coelurosaurian theropods in South America and adds valuable information about the paleoecologies of non-avian theropod dinosaurs, particularly in the early Late Cretaceous of Patagonia. ","palabras_clave":"THEROPODA|BEHAVIOUR|BAJO BARREAL FORMATION|LATE CRETACEOUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63381,"idioma_id":4,"titulo":"An Intact Brachyury Function Is Necessary to Prevent Spurious Axial Development in Xenopus laevis","resumen":"We have previously shown that the member of the HES family hairy2 induces the ectopic expression of dorsal markers when it is overexpressed in the ventral side of Xenopus embryos. Intriguingly, hairy2 represses the mesoderm transcription factor brachyury (bra) throughout its domain in the marginal zone. Here we show that in early gastrula, bra and hairy2 are expressed in complementary domains. Overexpression of bra repressed hairy2. Interference of bra function with a dominant-negative construct expanded the hairy2 domain and, like hairy2 overexpression, promoted ectopic expression of dorsal axial markers in the ventral side and induced secondary axes without head and notochord. Hairy2 depletion rescued the ectopic dorsal development induced by interference of bra function. We concluded that an intact bra function is necessary to exclude hairy2 expression from the non-organiser field, to impede the ectopic specification of dorsal axial fates and the appearance of incomplete secondary axes. This evidence supports a previously unrecognised role for bra in maintaining the dorsal fates inhibited in the ventral marginal zone, preventing the appearance of trunk duplications.","palabras_clave":"BRACHYURY|DORSAL MIDLINE|NOTCH|VERTEBRATE DEVELOPMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63382,"idioma_id":4,"titulo":"Autoantibodies against muscarinic receptors in breast cancer. Their role in tumor angiogenesis.","resumen":"The presence of autoantibodies in cancer has become relevant in recent years. We demonstrated that autoantibodies purified from the sera of breast cancer patients activate muscarinic acetylcholine receptors in tumor cells. Immunoglobulin G (IgG) from breast cancer patients in T1N0Mx stage (tumor size¡Ü2 cm, without lymph node metastasis) mimics the action of the muscarinic agonist carbachol stimulating MCF-7 cell proliferation, migration and invasion. Angiogenesis is a central step in tumor progression because it promotes tumor invasion and metastatic spread. Vascular endothelial growth factor-A (VEGF-A) is the main angiogenic mediator, and its levels have been correlated with poor prognosis in cancer. The aim of the present work was to investigate the effect of T1N0Mx-IgG on the expression of VEGF-A, and the in vivo neovascular response triggered by MCF-7 cells, via muscarinic receptor activation. We demonstrated that T1N0Mx-IgG (10&#8722;8 M) and carbachol (10&#8722;9 M) increased the constitutive expression of VEGF-A in tumor cells, effect that was reverted by the muscarinic antagonist atropine. We also observed that T1N0Mx-IgG and carbachol enhanced the neovascular response produced by MCF-7 cells in the skin of NUDE mice. The action of IgG or carbachol was reduced in the presence of atropine. In conclusion, T1N0Mx-IgG and carbachol may promote VEGF-A production and neovascularization induced by breast tumor cells via muscarinic receptors activation. These effects may be accelerating breast tumor progression.","palabras_clave":"Autoantibodies|muscarinic receptors|angiogenesis|breast cancer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63383,"idioma_id":4,"titulo":"Evidence for extensive genetic diversity and sub-structuring of the Babesia bovis metapopulation","resumen":"Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and minisatellite markers was used to estimate population genetic parameters of eighteen parasite isolates originating from different continents, countries and geographic regions including North America (Mexico, USA), South America (Argentina, Brazil), the Middle East (Israel) and Australia. For eleven of the eighteen isolates, a unique haplotype was inferred suggesting selection of a single genotype by either in vitro cultivation or amplification in splenectomized calves. Furthermore, a high genetic diversity (H = 0.780) over all marker loci was estimated. Linkage disequilibrium was observed in the total study group but also in sample subgroups from the Americas, Brazil, and Israel and Australia. In contrast, corresponding to their more confined geographic origin, samples from Israel and Argentina were each found to be in equilibrium suggestive of random mating and frequent genetic exchange. The genetic differentiation (FST) of the total study group over all nineteen loci was estimated by analysis of variance (&#920;) and Nei?s estimation of heterozygosity (GST?) as 0.296 and 0.312, respectively. Thus, about 30% of the genetic diversity of the parasite population is associated with genetic differences between parasite isolates sampled from the different geographic regions. The pairwise similarity of multilocus genotypes (MLGs) was assessed and a neighbour-joining dendrogram generated. MLGs were found to cluster according to the country\/continent of origin of isolates, but did not distinguish the attenuated from the pathogenic parasite state. The distant geographic origin of the isolatesstudied allows an initial glimpse into the large extent of genetic diversity and differentiation of the B. bovis population on a global scale.","palabras_clave":"bovine babesiosis|satellite markers|population structure|genetic diversity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63384,"idioma_id":5,"titulo":"ACOMPAÑAR EL PROCESO DE INICIACION EN INVESTIGACION DE JOVENES ESTUDIANTES DE GRADO Y POSGRADO","resumen":"Este artículo se intenta describir y comprender las características del trabajo que los docentes-investigadores realizan al acompañar a jóvenes estudiantes de grado y posgrado en el proceso de iniciación en la formación en investigación. Surge de la reflexión que los autores producen sobre su propia experiencia como tutores de jóvenes investigadores, en distintas universidades y programas de investigación de nuestra región. Propone una modalidad de acompañamiento basado en la incentivación del aprendizaje en dos dimensiones: la dimensión humana-social y la dimensión intelectual-operativa, a partir de un dispositivo de supervisión que revaloriza el lugar de la experiencia personal y emotiva.","palabras_clave":"investigadores|supervision|jovenes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63385,"idioma_id":5,"titulo":"Lista de los peces de la Provincia de La Rioja","resumen":"Los censos de la fauna de un país o región cualquiera, constituyenuna suerte de codificación de la Naturaleza, como paso previo eindispensable para un ensayo racional de aprovechamiento de lasriquezas naturales y de su adecuada conservación. Desde fines delsiglo XVIII se ha dicho, y lo han repetido en nuestro medio, CosmeArgerich, Alberto Palcos y otros estudiosos y cientistas, que unCódigo de la Naturaleza es indispensable para la libertad de unanación. [R. A. Ringuelet & R. H. Arámburu. Enumeraciónsistemática de los vertebrados de la Provincia de Buenos Aires. LaPlata, MAA, Publ. 119, 1957].","palabras_clave":"Ictiofauna|La Rioja|Lista"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63386,"idioma_id":4,"titulo":"Strong correlation between molecular configurations and charge-transfer processes probed at the single molecule level by SERS","resumen":"Single-molecule (SM) electrochemistry studied  by surface-enhanced Raman scattering (SERS) with high  spectral resolution reveals a picture in which the frequency of  Raman modes is correlated with the electrochemical process  through the interaction with the surface. Previously unexplored  phenomena can be revealed by the synergy of electrochemistry  and SM-SERS, which explores in this case subtler spectroscopic aspects (like the frequency of a vibration within the  inhomogeneous broadening of a many-molecules Raman  peak) to gain the information. We demonstrate, among  other things, that the interaction with the surface is correlated both with the molecule vibrational frequencies and with the ability  of single molecules to be reduced\/oxidized at di&#64256;erent potentials along the electrochemical cycle. Qualitative models of the  interaction of molecules with surfaces are also touched upon.","palabras_clave":"Electrochemistry|SERS|Single-molecule|Charge transfer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63387,"idioma_id":4,"titulo":"Cajal bodies are developmentally regulated during pollen development and pollen tube growth in Arabidopsis thaliana","resumen":"Cajal bodies (CBs) are sub-nuclear bodies first described in neurons byRamon y Cajal in 1903 and subsequently characterized in both plant and animals(Gall, 2003). They appear close to the nucleoli as discrete foci whose size andnumber vary during the cell cycle (Strzelecka et al., 2010). They are dynamicstructures that move, fuse and separate depending on the transcriptional statusof the cell (Cioce and Lamond, 2005). CBs are involved in assembly andtrafficking of small nucleolar ribonucleoproteins (snoRNPs) and in assembly andtrafficking of spliceosomal small nuclear ribonucleoprotein (snRNPs) complexesthat are involved in mRNA splicing (Cioce and Lamond, 2005; Gall, 2003). Cajalbodies also contain components of the machinery involved in siRNA-mediatedsilencing and in methylation of repetitive DNA (Li et al., 2006). Vertebrate Cajalbodies contain a high concentration of coilin, a protein that is widely used as amolecular marker for CBs (Cioce and Lamond, 2005). In Arabidopsis there is adistant homolog of vertebrate coilin (Collier et al., 2006). An Arabidopsis mutant,ncb1 (no cajal bodies 1), lacks CBs due to a single base change at a splice site inArabidopsis coilin (At1g13030), resulting in total disassembly of CBs (Collier etal., 2006; Fang and Spector, 2007). A tagged version of Arabidopsis coilinrestored the formation of CBs in ncb-1 (Collier et al., 2006). Despite thefunctional relevance of CBs in RNA processing, knock-out mutants for coilin havebeen obtained in mice, although they exhibit reduced viability (Walker et al.,2009), whereas Arabidopsis and Drosophila that lack CBs are completely viableand normal (Collier et al., 2006; Liu et al., 2009). These results therefore suggestthat the processes that normally occur in the CBs can be carried out in thenucleoplasm.","palabras_clave":"cajal bodies"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63388,"idioma_id":4,"titulo":"Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum","resumen":"Bioremediation of contaminated sites by biosorption of pollutants onto a wide range of materials has  emerged as a promising treatment for recalcitrant aromatic compounds or heavy metals. When  adsorption occurs on living white-rot fungi mycelia, the pollutants may be degraded by ligninolytic  enzymes. However, the survival of mycelia in harsh conditions is one of the drawbacks of those methodologies.  In this study, it was demonstrated that culture media supplemented with several guaiacol  derivatives (vanilloids) increased the resistance of Ganoderma lucidum E47 cultures to chemical stress by  enhancing the adsorptive capacity of the extracellular mucilaginous material (ECMM). The toxicity of the  fungicides gentian violet (GV), malachite green (MG) and clotrimazole, and the heavy metal Cadmium  was noticeably diminished in fungal cultures supplemented with the guaiacol derivative vanillic acid  (VA). No degradation of the tested compounds was detected. The activity of the oxidative enzymatic  systems like laccase, a well-known oxidase associated to dye degradation, was only detectable after  complete growth on plates. Extremely low concentrations of VA caused a signi!cant protective effect,  radial extension of the growth halo in plates supplemented with 0.0001 mM of VA plus GV was up to 20%  to that obtained in control plates (without addition of GV and VA). Therefore, the protective effect could  not be attributable to VA per se. ECMM separated from the mycelium exhibited a much higher increase in  the adsorptive capacity when isolated from liquid cultures containing VA, while that obtained from  unsupplemented cultures showed an almost null adsorptive capacity.  ! 2013 Elsevier Ltd. All rights reserved","palabras_clave":"BIOREMEDIATION|BIOSORPTION|EXOPOLYSACCHARIDE|FUNGAL GROWTH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63390,"idioma_id":4,"titulo":"New records of Geastrum Pers. (Phallomycetidae, Geastrales) from Argentina","resumen":"In the present study two new records of Geastrum are reported for Argentina: G. hirsutum Baseia & Calonge and G. setiferum Baseia. Descriptions, comments and illustrations of basidiomata are given.","palabras_clave":"Argentina|Geastrales|mycobiota|phallomycetidae|taxonomy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63391,"idioma_id":5,"titulo":"DESARROLLO DE UN ELISA INDIRECTO PARA CUANTIFICAR ANTICUERPOS BOVINOS CONTRA PARAINFLUENZA VIRUS 3","resumen":"Parainfluenza virus tipo 3 (PIV3) es un virus envuelto de ARN simple cadena y polaridad negativo, pertenece al género Respirovirus y a la familia Paramixoviridae. Este virus ocasiona graves pérdidas en términos de morbilidad y reducción de la producción en el ganado vacuno. El cuadro clínico de la infección con parainfluenza 3 bovino puede variar considerablemente desde una infección asintomática a una severa enfermedad respiratoria. En nuestro país la infección por PIV3 fue detectada por evidencia serológica en la década del 80\u2019. Relevamientos serológicos realizados en la década del 2000 en rodeos bovinos no vacunados de Jujuy y Neuquén indicaron que el 100% de los bovinos adultos resultaron seropositivos para anticuerpos contra este agente viral. Además el virus se ha aislado de casos de enfermedad respiratoria de bovinos y de búfalos. Las cepas detectadas en bovinos se clasificaron dentro de los genotipos A y C, mientras que las cepas detectadas en búfalos correspondieron a un virus bovino perteneciente al genotipo B. Con éste hallazgo, resultó necesario conocer el estatus inmunológico actual de rodeos bovinos argentinos. La técnica gold standards para la determinación de anticuerpos anti PIV3 es la inhibición de la hemoaglutinación (IH), sin embargo, esta técnica resulta poco efectiva por su falta de objetividad. Por lo tanto, se describe en el presente trabajo el desarrollo de un ELISA indirecto para evaluar anticuerpos en sueros bovinos.   El ELISA indirecto se estandarizó para cuantificar anticuerpos contra virus parainfluenza 3 de bovino de los tres genotipos semipurificados e inmovilizados en la placa. Las condiciones óptimas de este ELISA fueron incubación con PBSt-OVA 1 % como solución de bloqueo y una hora de incubación para los sueros problema. La técnica de ELISA indirecta fue comparada con la técnica de IH con un grupo de sueros bovinos y se pudo observar que la técnica que se describe en éste trabajo, detecta el mismo número de sueros positivos y negativos que la IH pudiéndose utilizar ésta como complementaria al ensayo de IH. Para establecer el punto de corte se realizó una curva de frecuencia con sueros positivos y negativos de historia conocida. El punto de corte se determinó en una densidad óptica corregida mayor o igual a 0.4 a partir de la cual se consideran los sueros positivos. El ELISA desarrollado resulta una técnica rápida, cuantitativa y complementaria a la IH para cuantificar anticuerpos contra PIV3 de bovinos y podría estar disponible comercialmente en el país en un futuro cercano.","palabras_clave":"Parainfluenza virus 3|ELISA indirecto|anticuerpos en bovinos|diagnóstico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63392,"idioma_id":5,"titulo":"Mapeo de QTL para resistencia a mal de Rio Cuarto en maiz","resumen":"La enfermedad más importante del maíz en la Argentina es el Mal de Río Cuarto (MRC). Producida por el virus identificado como Mal de Río Cuarto virus (MRCV) género Fijivirus, Familia Reoviridae, es una enfermedad endémica de la región central de Argentina. Estudios iniciales mostraron que la resistencia al MRCV es un carácter oligogénico de moderada a baja heredabilidad, regido por genes nucleares con efectos aditivos y dominantes. Los genotipos usualmente tienen respuestas impredecibles porque esta enfermedad virósica, que se presenta de manera no sistemática, manifiesta una marcada interacción genotipo-ambiente. El mapeo de QTL para la reacción al MRCV permitió determinar el número y el efecto individual de loci involucrados y estimar las interacciones entre estos genes y de ellos con el ambiente. Los resultados obtenidos con diferentes poblaciones de mapeo, fondos genéticos y métodos de análisis estadísticos indican que los QTL identificados en la mayoría de los ambientes de evaluación se ubican en las mismas regiones cromosómicas en las que se han detectado loci de resistencia a otros patógenos agrupados en racimo (cluster). Los QTL para reacción a MRCV están ubicados en los cromosomas 1, 3, 4, 6, 8 y 10, los cuales explican entre 6 y 42 % de la variación fenotípica según las poblaciones, los ambientes y los métodos de análisis. En particular, los QTL de los bins 1.02\/3, 8.02\/3 y 10.02\/4 podrían considerarse promisorios para detectar genes candidatos relacionados con esta enfermedad virósica. Estudios adicionales permitirán su utilización en mejoramiento genético, conservación de germoplasma y análisis genómico.","palabras_clave":"loci de caracteres cuantitativos|enfermedad viral|MRCV|marcadores moleculares"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63393,"idioma_id":5,"titulo":"Mapeo de QTL para una medida multivariada de la reacción al virus del Mal de Río Cuarto","resumen":"El índice de severidad de enfermedad (ISE), como indicador de   resistencia multidimensional, es una media ponderada de la severidad e   incidencia de la enfermedad del maíz causada por el Mal de Río Cuarto   Virus (MRCV). Un mapeo por intervalo se realizó con el objetivo de   identificar QTLs asociados con el ISE del Mal de Río Cuarto (MRC) en una   población de líneas endocriadas recombinantes (RILs), las que fueron   evaluadas en ambientes donde la enfermedad es endémica. Los resultados   sugieren algunas regiones genómicas (cromosomas 1, 4, 6, 8 y 10) con   efecto significativo sobre la reacción al MRCV. Alrededor del 40% de los   intervalos significativos coinciden con aquellos identificados en   estudios previos. Por otro lado, se identificaron intervalos no   detectados cuando el análisis se realiza carácter por carácter. Los   resultados sugieren que es posible realizar la selección de genotipos   resistentes al virus utilizando los síntomas particulares de la   enfermedad así como mediante el uso de QTLs asociados al MRC.  Palabras   clave: maíz, líneas endocriadas recombinantes, índice de severidad de   enfermedad, microsatélites, enfermedad viral.","palabras_clave":"QTLS|MARCADORES MOLECULARES|MAL DE RIO CUARTO|INDICE DE SEVERIDAD DE ENFERMEDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63394,"idioma_id":4,"titulo":"Swarming motility in Bradyrhizobium japonicum","resumen":"Flagella-driven bacterial motility is an important trait for colonization of natural environments. Bradyrhizobium japonicum is a soil species that possesses two different flagella systems: one subpolar and the other lateral, each one with a filament formed by a different set of flagellins. While the synthesis of subpolar flagellins was constitutive, translation of lateral flagellins was detected in rhizobia grown with L-arabinose but not with D-mannitol as sole carbon-source, independently of whether bacteria were in liquid or semisolid medium. We characterized swarming of B. japonicum in semisolid medium and found that this motility was faster with L-arabinose than with D-mannitol. By using mutants with deletions in each flagellin set, we evaluated the contribution of each flagella system to swarming in semisolid culture media, and in soil. Mutants devoid of either of the flagella were affected in swarming in culture media, being this impairment stronger for mutants without lateral flagellum. In sterile soil at 100 % or 80 % field capacity, flagella-driven motility of mutants able to swim but impaired in swarming was similar to wild-type, indicating that swimming was the predominant movement here.","palabras_clave":"Swarming|Motility|Flagella|Bradyrhizobium"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63395,"idioma_id":5,"titulo":"Dopamina Renal: nuevos roles con implicancias clínicas en nefroprotección y regulación de la presión arterial","resumen":"La dopamina (DA) renal modula la excreción de sodio y agua y la presión arterial por medio de receptores D1 (D1R) y D2 y es degradada por las enzimas monoamino-oxidasa (MAO) y catecol-O-metiltransferasa (COMT). Nuestro propósito es estudiar el patrón de excreción urinaria de DA (UDAV) y la actividad de MAO y COMT durante el consumo de dietas con distinto contenido de sodio. Para ello, ratas Wistar macho, divididas en 4 grupos, recibieron dieta normosódica (NS, NaCl 0.24%), hiposódicas (BS, NaCl 0.02%) e hipersódicas (HS, NaCl 1%) durante 5 días. El 4to grupo, NS, BS e HS, recibió SCH 23390 (antagonista D1R, 1 mg\/kg SC) los últimos 3 días. Observamos que UDAV (ng\/d\/100gPC) en BS fue más baja que NS: 571.2±30.3 vs. 730±45, p<0.01, y aumentó en HS: 1443±203.2, p<0.01vs. NS. En BS la actividad de MAO (nmol\/ mg \/ hora) aumentó en corteza (C) 9.44±0.55 respecto a NS, 7.66±0.52, p<0.05. COMT, (pmol\/mg \/hora) aumentó en C, 15.67±1.23 vs. 11.4±0.93, p<0.05 vs. NS y disminuyó en médula (M) 7.79±0.33 vs. 9.84±0.31, p<0.01 vs. NS. En HS  MAO disminuyó en C: 6.3±0.25 vs. 7.66±0.52, p<0.05 vs NS; en tanto COMT disminuyó algo en M. No hubo cambios en la presión arterial sistólica (PAS) basal entre los grupos. El tratamiento con SCH 23390 disminuyó la natriuresis en HS y NS, y aumentó significativamente la PAS en BS. En conclusión, DA y natriuresis, exhiben una correlación positiva frente a modificaciones en la ingesta de sodio, respuesta  modulada por MAO y COMT y mediada por D1R.","palabras_clave":"DOPAMINA|PRESIÓN ARTERIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63396,"idioma_id":4,"titulo":"Flowering regulation in the facultative biennial Oenothera biennis L.: Environmental effects and their relation to growth rate","resumen":"The lifecycle of the facultative biennial oilseed-crop evening primrose (Oenothera biennis) is a major constraint for its commercial production under different growing conditions, as a variable proportion of plants fails to flower during the first season and remains as vegetative rosettes (biennial behavior). The aim of this work was to understand how flowering is regulated in this species and to identify the main determinants of its biennial behavior. Different planting dates and manipulative treatments (seed vernalization, photoperiod extension and fertilization) were employed to analyze if: i) biennial behavior occurs when obligate requirements for vernalization or photoperiod are not satisfied; and ii) responses to these environmental cues depend on the size and\/or growth rate of rosettes. Our results indicate that O. biennis has an obligate long-day requirement for flowering and a facultative vernalization response. There is no minimum size requirement for vernalization response (as very small seedlings responded to the vernalization treatment) and the rate of development towards flowering under inductive photoperiods was strongly affected by rosette\u2019s growth rate. The incidence of high temperatures just before the onset of reproduction is proposed as an inhibitory factor that prevents reproduction under otherwise photo-inductive conditions. This last factor would explain the high incidence of biennial behavior frequently observed in spring\/summer sowing in this crop.","palabras_clave":"Oenothera biennis| Flowering| Photoperiod|High temperature"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63397,"idioma_id":4,"titulo":"Comparing methods for extracting amaranthus starch and the properties of the isolated starches","resumen":"Starch was isolated from Amaranthus cruentus whole grain (WG) and whole grain flour (WGF) using both the alkaline method (AM) and AM combined with food degree protease digestion (AMP). The methods involved successive soaking in NaOH solution (0.25g\/100ml in AM and 0.05g\/100ml in AMP), fibrous fraction wet milling, enzymatic hydrolysis in AMP and multi-staged centrifugation. Milling the amaranth grains in both methods increased significantly starch yield, recovery, and purity when compared against WG and lowered soaking times as well. Starch yield and recovery were 116.7% and 123.6% higher in WGF while protein, fiber, and ash contents showed decreases of about 44.4%, 34.8%, and 30.4% respectively. The effect of the extracting methods was less notorious than that of the grain milling. The results suggest that both methods are suitable for extracting starch from previously milled grains despite the fact that the AM shows significant operative advantages. The starches extracted showed conservative structure, A-type difractrometric patterns with high crystallinity degrees (~39%) and Tg (gelatinization temperature) values (~74ºC). These properties not present significant differences as a consequence of the extracting procedure used.","palabras_clave":"amaranth starch|cristallinity|thermal properties|extraction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63398,"idioma_id":4,"titulo":"Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian native forest","resumen":"Cellulolytic activities of three bacterial consortia derived from a   forest soil sample from Chaco region, Argentina, were characterized. The   phylogenetic analysis of consortia revealed two main highly supported   groups including Achromobacter and Pseudomonas   genera. All three consortia presented cellulolytic activity. The   carboxymethylcellulase (CMCase) and total cellulase activities were   studied both quantitatively and qualitatively and optimal enzymatic   conditions were characterized and compared among the three consortia.   Thermal and pH stability were analyzed. Based on its cellulolytic   activity, one consortium was selected for further characterization by   zymography. We detected a specific protein of 55&nbsp;kDa with CMCase   activity. In this study, we have shown that these consortia encode for   cellulolytic enzymes. These enzymes could be useful for lignocellulosic   biomass degradation into simple components and for different industrial   applications.","palabras_clave":"CHARACTERIZATION|CELLULOLYTIC|BACTERIAL|CONSORTIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63399,"idioma_id":4,"titulo":"A BDNF sensitive mechanism is involved in the fear memory resulting from the interaction between stress and the retrieval of an established trace","resumen":"The present study investigates the fear memory resulting from the interaction of a stressful experience and the retrieval ofan established fear memory trace. Such a combination enhanced both fear expression and fear retention in adult Wistar rats.Likewise, midazolam intra-basolateral amygdala (BLA) infusion prior to stress attenuated the enhancement of fear memorythus suggesting the involvement of a stress-induced reduction of the GABAergic transmission in BLA in the stress-inducedenhancing effect. It has been suggested that, unlike the immediate-early gene Zif268 which is related to the reconsolidationprocess, the expression of hippocampal brain-derived neurotrophic factor (BDNF) is highly correlated with consolidation.We therefore evaluate the relative contribution of these two neurobiological processes to the fear memory resulting fromthe above-mentioned interaction. Intra-dorsal hippocampus (DH) infusions of either the antisense Zif268 or the inhibitor ofthe protein degradation (Clasto-Lactacystin b-Lactone), suggested to be involved in the retrieval-dependent destabilization process, did not affect the resulting contextual memory. In contrast, the knockdown of hippocampal BDNF mitigated thestress-induced facilitating influence on fear retention. In addition, the retrieval experience elevated BDNF level in DH at 60min after recall exclusively in stressed animals. These findings suggest the involvement of a hippocampal BDNF sensitivemechanism in the stress-promoting influence on the fear memory following retrieval.process, did not affect the resulting contextual memory. In contrast, the knockdown of hippocampal BDNF mitigated thestress-induced facilitating influence on fear retention. In addition, the retrieval experience elevated BDNF level in DH at 60min after recall exclusively in stressed animals. These findings suggest the involvement of a hippocampal BDNF sensitivemechanism in the stress-promoting influence on the fear memory following retrieval.process, did not affect the resulting contextual memory. In contrast, the knockdown of hippocampal BDNF mitigated thestress-induced facilitating influence on fear retention. In addition, the retrieval experience elevated BDNF level in DH at 60min after recall exclusively in stressed animals. These findings suggest the involvement of a hippocampal BDNF sensitivemechanism in the stress-promoting influence on the fear memory following retrieval.b-Lactone), suggested to be involved in the retrieval-dependent destabilization process, did not affect the resulting contextual memory. In contrast, the knockdown of hippocampal BDNF mitigated thestress-induced facilitating influence on fear retention. In addition, the retrieval experience elevated BDNF level in DH at 60min after recall exclusively in stressed animals. These findings suggest the involvement of a hippocampal BDNF sensitivemechanism in the stress-promoting influence on the fear memory following retrieval.","palabras_clave":"BDNF|Fear memory|Stress|retrieval"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63400,"idioma_id":4,"titulo":"Comparative reproductive biology of Sciaenidae family species in the Río de la Plata and Buenos Aires Coastal Zone, Argentina","resumen":"A comparative analysis on various aspects of the reproductive biology of 7 Sciaenidae family species was done. In addition provides the reproductive parameters needed for stock assessment models. The research covered the analysis of the duration of the breeding season and the estimation of reproductive variables such as size at maturity, fecundity and spawning frequency. When all species were considered together, some aspects of reproduction were similar, regarding to the reproductive strategy; all species are batch spawners with indeterminate fecundity and an extensive reproductive season (approximately six months) except of Pogonias cromis, which showed a more limited spawning season (three month). With regard to reproductive potential, wide differences in fecundity values mainly due to the different sizes attained by each species where observed (range in batch fecundity values between 5,000 and 2,800,000 hydrated oocytes). However, when relative fecundity was compared, average values were generally similar, ranging approximately between 150 and 200 hydrated oocytes per female gram. P. cromis is the exception within this group, because the mean relative fecundity ranged between 75 and 98 hydrated oocytes per ovary-free female gram. This difference may be a consequence of the greater oocyte size reached by P.cromis eggs (1100 - 1400 &#956;m) compared to the rest of sciaenid analyzed (750-900).","palabras_clave":"SCIAENIDAE|REPRODUCTIVE STRATEGY|REPRODUCTIVE POTENTIAL|LENGTH AT MATURITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63401,"idioma_id":4,"titulo":"Seasonal analysis of condition, biochemical and bioenergetic indices of females of Brazilian flathead, Percophis brasiliensis","resumen":"Percophis brasiliensis is a demersal species that constitutes an important resource of Argentine coastal fisheries. Nevertheless, information about bioenergetic dynamic of reproduction has not been reported. Therefore, seasonal variations of condition factors, biochemical composition and energy density of different tissues were analyzed in order to determine the strategy of energy allocation during the reproductive cycle of this species. Condition indices (hepatosomatic and K) showed a seasonal pattern opposite to that observed for gonadosomatic index, which was characterized by higher values during the reproductive period (spring-summer), decreasing at the end of spawning. Biochemical composition of different tissues also showed a clearly seasonality associated to reproductive cycle. Analysis of energy density variation of liver indicates that P. brasiliensis accumulate reserves in winter before reproduction, which later decrease during the spawning season. In contrast, the energy density in muscle did not show significant differences among seasons, indicating that individuals could be also using an external source of energy during spawning. Therefore, it is possible that P. brasiliensis respond to an intermediate strategy of energy allocation, combining characteristics of both capital breeders (stores energy previous to the onset of reproductive activity) and income breeders (acquire energy by active feeding during spawning period).","palabras_clave":"Percophis brasiliensis|CONDITION|ENERGY|REPRODUCTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63402,"idioma_id":4,"titulo":"SAFETY AND THERAPEUTIC EFFECT OF METRONOMIC CHEMOTHERAPY WITH CYCLOPHOSPHAMIDE AND CELECOXIB IN ADVANCED BREAST CANCER PATIENTS","resumen":"Metronomic chemotherapy (MCT), the chronic administration, at regular intervals, of low doses of chemotherapeutic drugs without extended rest periods, allows chronic treatment with therapeutic efficacy and low toxicity. Our preclinical results suggested that combined MCT with cyclophosphamide and celecoxib could inhibit breast cancer growth. The aim of this study was to determine the toxicity, safety and efficacy of oral MCT with cyclophosphamide 50 mg per orem daily and celecoxib 400 mg (200 mg per orem two-times a day) in advanced breast cancer patients. During the first stage of the study, the therapeutic response consisted of prolonged stable disease for &#8805;24 weeks in six out of 15 (40%) patients with a median duration of 37.5 weeks and a partial response in one out of 15 (response rate: 6.7%) patients lasting 6 weeks. The overall clinical benefit rate was 46.7%. The median time to progression was 14 weeks. Progression-free survival at 24 weeks was 40% and the 1-year overall survival rate was 46.7%. The adverse events were mild (gastric, grade 1; and hematologic, grade 1 or 2). No grade 3 or 4 toxicities were associated with the treatment. Evaluation of patients´ quality of life showed no changes during the response period. MCT with cyclophosphamide plus celecoxib is safe and shows a therapeutic effect in advanced breast cancer patients.","palabras_clave":"Breast cancer|Metronomic chemotherapy|cyclophosphamide|celecoxib|Biomarker"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63403,"idioma_id":4,"titulo":"Analysis of novel mycobacteriophages indicates the existence of different strategies for phage inheritance in mycobacteria","resumen":"Mycobacteriophages have been essential in the development of mycobacterial genetics through their use in the construction of tools for genetic manipulation. Due to the simplicity of their isolation and variety of exploitable molecular features, we searched for and isolated 18 novel mycobacteriophages from environmental samples collected from several geographic locations. Characterization of these phages did not differ from most of the previously described ones in the predominant physical features (virion size in the 100\u2013400 nm, genome size in the 50\u201370 kbp, morphological features compatible with those corresponding to the Siphoviridae family), however novel characteristics for propagation were noticed. Although all the mycobacteriophages propagated at 30°C, eight of them failed to propagate at 37°C. Since some of our phages yielded pinpoint plaques, we improved plaque detection by including sub-inhibitory concentrations of isoniazid or ampicillin-sulbactam in the culture medium. Thus, searches for novel mycobacteriophages at low temperature and in the presence of these drugs would allow for the isolation of novel members that would otherwise not be detected. Importantly, while eight phages lysogenized Mycobacterium smegmatis, four of them were also capable of lysogenizing Mycobacterium tuberculosis. Analysis of the complete genome sequence obtained for twelve mycobacteriophages (the remaining six rendered partial genomic sequences) allowed for the identification of a new singleton. Surprisingly, sequence analysis revealed the presence of parA or parA\/parB genes in 7\/18 phages including four that behaved as temperate in M. tuberculosis. In summary, we report here the isolation and preliminary characterization of mycobacteriophages that bring new information to the field.","palabras_clave":"MYCOBACTERIUM|TUBERCULOSIS|MYCOBACTERIOPHAGES|GENOME ORGANIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63404,"idioma_id":4,"titulo":"Cretaceous cicatricose spores from north central-western Argentina:Taxonomic and biostratigraphic discussion","resumen":"Cicatricose spores have been broadly described from Cretaceous worldwide basins. A complete revision of previous records from Argentina and other parts of South America is presented here, as well as the results of a detailed taxonomic study of the species found in the La Yesera and Lagarcito formations (north and central western Argentina, respectively). Four genera and 11 species are described: Cicatricosisporites cuneiformis, C. pramparoana, C. sp. 1, Fisciniasporites brevilaesuratus, F. sp., Nodosisporites sp., Ruffordiaspora australiensis, R. cardielensis, R. ludbrookiae, R.cf. R. ticoensis and R. sp. 1.The genus Fisciniasporites is recorded for the first time in Argentina (La Yesera Formation), and Nodosisporitesin the San Luis Basin.The abundance of the cicatricose spores is higher in the La Yesera Formation (8-54.2% of the total assemblage) than in the Lagarcito Formation (2-16 %). An acme of cicatricose spores diversity in Argentina was recognized during the Aptian-Albian interval. Taxonomic studies of these types of spores are very important as they constitute a useful contribution of accurate descriptions and illustrations within a morphologic group that has many misidentifications, and also considering the biostratigraphic significance of some species, e.g. Cicatricosisporites cuneiformis in Australia.","palabras_clave":"CICATRICOSE SPORES|ANEMIACEAE|CRETACEOUS|SALTA GROUP BASIN,SAN LUIS BASIN, ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63405,"idioma_id":4,"titulo":"Therapeutic efficacy of metronomic chemotherapy with cyclophosphamide and doxorubicin on murine mammary adenocarcinomas","resumen":"BACKGROUND: Metronomic chemotherapy (MCT) refers to the chronic and equally spaced administration of low doses of different chemotherapy drugs, without extended rest periods. Herein, we investigated the therapeutic efficacy of metronomic cyclophosphamide (Cy) combined with doxorubicin (Dox) in two mouse mammary adenocarcinoma models. MATERIALS AND METHODS: Mice were s.c. challenged with M-234p or M-406 mammary tumors, and when the tumors reached approximately 150 mm(3), they were treated with: (I) no treatment (controls); (II) Cy in the drinking water (30 mg\/kg body weight\/day); (III) Dox (0.5 mg\/kg body weight i.p. three times\/week); (IV) treated as (II) + (III). Mice challenged i.v. with M-234p or M-406 tumor cells received, on day 3, the same treatments. RESULTS: We found that MCT with Cy plus Dox inhibited tumor growth, decreased lung metastases, and increased the median survival time, while having low toxic effect. Combined MCT was more effective than each monotherapy causing decrease in VEGF serum concentration and tumor proliferation rate plus increase in tumor apoptosis. CONCLUSION(S): The therapeutic benefits of combined MCT with Cy and Dox on mammary adenocarcinomas together with its low toxic effect profile suggest the possibility of future translation into the clinic.","palabras_clave":"angiogenesis|cyclophosphamide|doxorubicin|mammary adenocarcinomas|metronomic chemotherapy|toxic effec"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63406,"idioma_id":4,"titulo":"Uranium Uptake by Montmorillonite-Biomass Complexes","resumen":"Montmorillonite clays and biomass have noticeable metal sorption capacity. Clays or biomass are difficult toseparate from the solution when used as sorbent materials. A methodology to retain biomass and improve separation processes is to generate clay biopolymers matrices from fungal biomass grown on a natural Montmorillonite (MMT). The objective of this study is to generate and characterize clay biopolymers matrices and evaluate their uranium adsorption capacity. The generated clay biopolymers (BMMTs) were characterized through X-ray diffraction, measurement of the apparent diameter of particles, and electrophoretic mobility. Some BMMTs showed greater Uranium-specific adsorption capacity than that found for MMT. The X-ray diffraction analysis indicated that the Uranium was located partially in the clay interlayer. The BMMT surfaces were morenegatively charged than the MMT surface, thus favoring their uranium uptake. Also, immobilization of the biomass and better coagulation of the system were achieved. These preliminary studies indicate that BMMTs have a great potentiality for uranium uptake processes.","palabras_clave":"Uranium|Biomass|montmorillonite|Adsorption"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63407,"idioma_id":4,"titulo":"Three new genera in Chytridiales from aquatic habitats in Argentina","resumen":"Sampling for chytrids in a variety of habitats has resulted in pure cultures that whenanalyzed have yielded hypotheses of relationships based on molecular and zoosporeultrastructural markers. To extend our understanding of diversity of Chytridiales in easternArgentina and USA, we isolated and examined the morphology, ultrastructure and 28S andITS1-5.8S-ITS2 rDNA sequences of numerous chytrids from aquatic habitats from these tworegions. Three family-level lineages (Chytridiaceae, Chytriomycetaceae, family incertae sedis)In Press at Mycologia, preliminary version published on May 25, 2013 as doi:10.3852\/12-353Copyright 2013 by The Mycological Society of America.are represented in our molecular phylogeny, and three new genera (Avachytrium,Odontochytrium in Chytriomycetaceae, Delfinachytrium in family incertae sedis) are described.These findings of new genera and species emphasize the potential for discovery of additionaldiversity.","palabras_clave":"chytrid|morphology|phylogeny|systematics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63408,"idioma_id":4,"titulo":"Candida infections, causes, targets and resistance mechanisms. Traditional and alternative antifungal agents","resumen":"The genus Candida includes about 200 different species, but only a few species are human opportunistic pathogens and cause infections when the host becomes debilitated or immunocompromised. Candida infections can be superficial or invasive. Superficial infections often affect the skin or mucous membranes and can be treated successfully with topical antifungal drugs. However, invasive fungal infections are often life-threatening, probably due to inefficient diagnostic methods and inappropriate initial antifungal therapies. Here, we briefly review our current knowledge of pathogenic species of the genus Candida and yeast infection causes and then focus on current antifungal drugs and resistance mechanisms. An overview of new therapeutic alternatives for the treatment of Candida infections is also provided.","palabras_clave":"CANDIDA INFECTIONS|ANTIFUNGAL AGENTS|RESISTANCE MECHANISMS|ALTERNATIVE AGENTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63409,"idioma_id":4,"titulo":"Molecular fingerprints to identify Candida species","resumen":"A wide range of molecular techniques have been developed for genotyping Candida species. Among them, multilocus sequence typing (MLST) and microsatellite length polymorphisms (MLP) analysis have recently emerged. MLST relies on DNA sequences of internal regions of various independent housekeeping genes, while MLP identifies microsatellite instability. Both methods generate unambiguous and highly reproducible data. Here, we review the results achieved by using these two techniques and also provide a brief overview of a new method based on high-resolution DNA melting (HRM). This method identifies sequence differences by subtle deviations in sample melting profiles in the presence of saturating fluorescent DNA binding dyes.","palabras_clave":"CANDIDA SPECIES|POLYMORPHISMS ANALYSIS|RESISTANCE|FINGERPRINTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63410,"idioma_id":4,"titulo":"A large accumulation of avian eggs from the Late Cretaceous of Patagonia (Argentina) reveals a novel nesting strategy in Mesozoic birds","resumen":"We report the first evidence for a nesting colony of Mesozoic birds on Gondwana: A fossil accumulation in Late Cretaceous sediments mapped and collected from within the campus of the National University of Comahue, Neuquén City, Patagonia (Argentina). Here, Cretaceous ornithothoracine birds, almost certainly Enanthiornithes, nested in an arid, shallow basinal environment among sand dunes close to an ephemeral water-course. We mapped 65 complete, near-complete, and broken eggs across an area of more than 55m2 that were laid either singly, or occasionally in pairs, onto a sandy substrate. All eggs were found apparently in, or close to, their original nest site; they all occur within the same bedding plane and almost certainly represent the product of a single nesting season.&nbsp; Although there is no evidence for nesting structures, all but one of the Comahue eggs were placed upright in the sand with their pointed end downwards, a position that would have exposed the pole containing the air cell and precluded egg turning. This egg position is not seen in living birds, with the exception of the basal galliform megapods who place their eggs within mounds of vegetation, and reveals a novel nesting behaviour in Mesozoic Aves. ","palabras_clave":"PATAGONIAN CRETACEOUS|FOSSIL EGGS|ENANTIORNITHES|NESTING COLONY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63411,"idioma_id":4,"titulo":"Taphonomy and palaeoecology of singular Hauterivian-Barremian nerineoid shell beds from the Neuquén Basin, west-central Argentina","resumen":"Nerineoid shell beds are described for the first time from Lower Cretaceous deposits of southern South America. These come from carbonates near the top of the Agrio Formation in southern Mendoza Province, west-central Argentina. To envisage the origin of the nerineoid shell beds, a taphonomic study was carried out, which indicated that these represent within-habitat time-averaged, primary sedimentologic concentrations with a secondary biogenic imprint related to a relatively high local production of nerineoid shells. The associated palaeoenvironments were studied through a facies analysis of the carbonate succession including the shell beds. The carbonates were deposited in a homoclinal ramp system and depict a shallowing upward trend from mid to inner ramp. The individuals lived and accumulated in oolitic shoals within the inner ramp, in a shallow, well-lit, high-energy setting above fair-weather wave base. Substrate was oxygenated and loose. The nerineoids are shown to belong to one species of the genus Eunerinea, and through the functional morphology of the shells they are tentatively interpreted as infaunal or semi-infaunal. It is suggested that the recorded monospecific nerineoid shell beds indicate that the palaeoenvironmental conditions may have been favourable for the development of abundant populations of these gastropods in the northern part of the Neuquén Basin during a short time interval in the Hauterivian- Barremian boundary. This could have been related to a brief warming episode but other factors may have also been involved.&nbsp;","palabras_clave":"Argentina|Early Cretaceous|Gastropods|Nerineoids|Neuquén Basin|Shell beds|Taphonomy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63412,"idioma_id":5,"titulo":"Tsunamis en el lago Nahuel Huapi: historias de deslizamientos y erupciones","resumen":"En el lecho del lago Nahuel Huapi se preservan evidencias de erupciones, sismos y deslizamientos subacuáticos. Estudiar estos fenómenos nos permite evaluar los peligros naturales en las costas lacustres.","palabras_clave":"NAHUAL HUAPI|TSUNAMI|GEOSWATH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63413,"idioma_id":4,"titulo":"Effect of a dam on epilithic algal communities of a mountain stream: before-after dam construction comparison","resumen":"In this study we evaluated the effect of a dam on epilithic algal communities by analyzing community response after dam construction and by comparing community composition, structure and biomass upstream and downstream of the dam. Samples of epilithic algae and environmental data were collected at each site during high and low water periods before and after dam construction in Achiras Stream (Córdoba, Argentina). Ordinations showed modifications in algal assemblages after dam construction and downstream of the dam. Ordinations also suggested a loss of seasonality at the downstream site since the assemblages were similar between hydrological periods after dam construction. Our results also pointed out that dam construction affected not only the assemblages living at downstream site but also those in the upstream site. Indicator species, obtained by the Indicator Value method, showed that, after dam construction, there could have been an increase in nutrient concentration and a release of plankton from the impoundment. Abundance, richness and diversity were altered after dam construction as assessed by ANOVAs derived from a modified BACI Design. The proportion of earlysuccessional species was higher at the upstream site while late-successional species were dominant at the downstream site, as predicted. Current velocity was higher in the high water period upstream of the dam, with no differences between hydrological periods at the downstream site. So, lower fluctuations in discharge downstream of the dam may have helped succession advance, whereas at the upstream site, mainly during the high water period, floods appear to have caused sloughing of life forms from the outer layers of the biofilm, resetting the algal community to early successional stages. It may be concluded that the dam affected algal community and favored succession advance mainly by reducing current velocity and flow fluctuations.","palabras_clave":"Periphyton|regulated stream|detector species"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63414,"idioma_id":4,"titulo":"Galectin-1 controls cardiac inflammation and ventricular remodeling during acute myocardial infarction","resumen":"Galectin-1 (Gal-1), an evolutionarily conserved &#946;-galactoside-binding lectin, plays essential roles in the control of inflammation and neovascularization. Although identified as a major component of the contractile apparatus of cardiomyocytes, the potential role of Gal-1 in modulating heart pathophysiology is uncertain. Here, we aimed to characterize Gal-1 expression and function in the infarcted heart. Expression of Gal-1 was substantially increased in the mouse heart 7 days after acute myocardial infarction (AMI) and in hearts from patients with end-stage chronic heart failure. This lectin was localized mainly in cardiomyocytes and inflammatory infiltrates in peri-infarct areas, but not in remote areas. Both simulated hypoxia and proinflammatory cytokines selectively up-regulated Gal-1 expression in mouse cardiomyocytes, whereas anti-inflammatory cytokines inhibited expression of this lectin or had no considerable effect. Compared with their wild-type counterpart, Gal-1-deficient (Lgals1(-\/-)) mice showed enhanced cardiac inflammation, characterized by increased numbers of macrophages, natural killer cells, and total T cells, but reduced frequency of regulatory T cells, leading to impaired cardiac function at baseline and impaired ventricular remodeling 7 days after nonreperfused AMI. Treatment of mice with recombinant Gal-1 attenuated cardiac damage in reperfused AMI. Taken together, our results indicate a protective role for Gal-1 in normal cardiac homeostasis and postinfarction remodeling by preventing cardiac inflammation. Thus, Gal-1 treatment represents a potential novel strategy to attenuate heart failure in AMI.","palabras_clave":"GALECTIN 1|VENTRICULAR REMODELING|MYOCARDIAL INFARCTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63415,"idioma_id":5,"titulo":"Efectos del ejercicio intenso sobre la función ventricular basal y la respuesta inotrópica, cronotrópica y lusitrópica en ratones","resumen":"Resumen no disponible","palabras_clave":"EJERCICIO INTENSO|FUNCIÓN VENTRICULAR BASAL|RESPUESTA INOTRÓPICA, CRONOTRÓPICA Y LUSITRÓPICA|RATONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63416,"idioma_id":4,"titulo":"Beneficial effects of solar UV-B radiation on soybean yield mediated by reduced insect herbivory under field conditions","resumen":"Ultraviolet-B radiation  (UV-B: 280-315 nm) has damaging effects on cellular components and  macromolecules. In plants, natural levels of UV-B can reduce leaf area  expansion and growth, which can lead to reduced productivity and yield. UV-B  can also have important effects on herbivorous insects. Owing to the successful  implementation of the Montreal Protocol, current models predict that clear-sky levels  of UV-B radiation will decline during this century in response to ozone  recovery. However, because of climate change and changes in land use practices,  future trends in UV doses are difficult to predict. In the experiments reported  here, we used an exclusion approach to study the effects of solar UV-B  radiation on soybean crops, which are extensively grown in many areas of the  world that may be affected by future variations in UV-B radiation. In a first  experiment, performed under normal management practices (which included  chemical pest control), we found that natural levels of UV-B radiation reduced  soybean yield. In a second experiment, where no pesticides were applied, we  found that solar UV-B significantly reduced insect herbivory and, surprisingly,  caused a concomitant increase in crop yield. Our data support the idea that  UV-B effects on agro-ecosystems are the result of complex interactions  involving multiple trophic levels. A better understanding of the mechanisms  that mediate the anti-herbivore effect of UV-B radiation may be used to design  crop varieties with improved adaptation to the cropping systems that are likely  to prevail in the coming decades in response to agricultural intensification.","palabras_clave":"plant-insect interactions|agricultural intensification|crop density|Glycine max|phenolic compounds"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63417,"idioma_id":4,"titulo":"COLLABORATER FOR A CAR-LIKE VEHICLE DRIVEN BY A USER WITH VISUAL INATTENTION","resumen":"This paper proposes a control system applied to a car-like vehicle driven by a user. The controller is designed to mitigate the negative effects produced by possible visual distractions of him. In addition, the paper proposes to evaluate the user?s visual distraction defining a vector that has two components: one respect to the path and the other one respect to the obstacles. These elements can be computed on-line and are associated with two time delays that produce a similar effect of instability on the vehicle motion. The proposed scheme considers the distraction in the design through such delays. Finally, experiments using a car simulator are carried out.","palabras_clave":"VISUAL ATTENTION|DRIVER|SAFETY|DELAY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63418,"idioma_id":4,"titulo":"Towards systematic software reuse of GIS: Insights from a case study","resumen":"With the development and adoption of geographic information systems, there is an increasingly amount of software resources being stored or recorded as products to be reused. At the same time, complexity of geographic services is addressed through standardization, which allows developers reaching higher quality levels. In this paper, we introduce our domain-oriented approach to developing geographic software product lines focusing on the experiences collected from a case study. It was developed in the Marine Ecology Domain (Patagonia, Argentina) and illustrates insights of the process as well as design decisions behind the model in order to reach a better level of reuse.","palabras_clave":"Geographic  Information Systems|ISO 19119 std|Software product lines|Reused Services"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63419,"idioma_id":4,"titulo":"Comparative efficacy of commercial combs in removing head lice (Pediculus humanus capitis) (Phthiraptera: Pediculidae)","resumen":"The use of a fine comb for removing lice from thehead of the human host is a relevant tool both in the diagnosisof infestations and as part of an integrated control strategy ofhead lice. The effectiveness of a fine comb depends, in part, onthe design and material they are built. The aim of this studywas to compare in vivo the efficacy ofmetal and plastic combsthat are currently used in the removal of head lice and eggsworldwide. The space between comb teeth and the length was0.23 and 13 mm in KSL® plastic, 0.3 and 10.7 mm inNOPUCID® plastic, 0.15 and 31 mm in KSL® metal and0.09 and 37 mmin ASSY® metal. The assays were performedcomparing the combs in pairs: (a) KSL® vs. NOPUCID®plastic combs, (b) KSL® vs. ASSY® metal combs and (c)KSL® plastic comb vs. ASSY® metal comb. The most effectiveplastic comb was KSL®, removing a higher number ofindividuals of all stages. The most effective metal comb wasASSY®, removing more insects of all stages (except adults).The comparative test between KSL® plastic and ASSY®metal showed that ASSY® was the most effective in removinghead lice and their eggs","palabras_clave":"Head Lice|Pediculus humanus capitis|Control|Combs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63420,"idioma_id":4,"titulo":"A Brucella virulence factor targets macrophages to trigger B-cell proliferation","resumen":"Brucella spp. and Trypanosoma cruzi are two intracellular pathogens that have no evolutionary common origins but share a similar lifestyle as they establish chronic infections for which they have to circumvent the host immune response. Both pathogens have a virulence factor (prpA in Brucella and tcPrac in T. cruzi) that induces B-cell proliferation and promotes the establishment of the chronic phase of the infectious process. We show here that, even though PrpA promotes B-cell proliferation, it targets macrophages in vitro and is translocated to the cytoplasm during the intracellular replication phase. We observed that PrpA-treated macrophages induce the secretion of a soluble factor responsible for B-cell proliferation and identified nonmuscular myosin IIA (NMM-IIA) as a receptor required for binding and function of this virulence factor. Finally, we show that the Trypanosoma cruzi homologue of PrpA also targets macrophages to induce B-cell proliferation through the same receptor, indicating that this virulence strategy is conserved between a bacterial and a protozoan pathogen.","palabras_clave":"BACTERIA|BRUCELLA|LYMPHOCYTE|MYOSIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63421,"idioma_id":5,"titulo":"Ciudad segura. Vecindad, víctimas y gubernamentalidad. Notas sobre la campaña electoral del PRO en la Ciudad de Buenos Aires (2011)","resumen":"En lassociedades de seguridad el sujeto político interpelado es el ciudadano-víctimay el Estado se presenta como dador de un marco de protección a un ciudadano enriesgo. El artículo propone un estudio de estas lógicas en la comunicaciónpolítica argentina, a partir del análisis de piezas comunicacionales de lacampaña 2011 del Jefe de Gobierno de la Ciudad de Buenos Aires. Este caso permite identificarlos significados sobre la victimización, el peligro y la gubernamentalidad enel marco de una enunciación electoral centrada en la eficacia del Estado en elcontrol de los riesgos y la seguridad.","palabras_clave":"COMUNICACIÓN POLÍTICA|CAMPAÑAS|SEGURIDAD|VICTIMIZACIÓN|GUBERNAMENTALIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63422,"idioma_id":4,"titulo":"Enhanced removal of a pesticides mixture by single cultures and consortia of free  and immobilized Streptomyces strains","resumen":"Pesticides are normally used to control specific pests and to increase the productivity in crops; as a result, soils are contaminated with mixtures of pesticides. In this work the ability of Streptomyces strains (either as pure or mixed cultures) to remove pentachlorophenol and chlorpyrifos was studied. The antagonism among the strains and their tolerance to the toxic mixture were evaluated. Results revealed that the strains did not have any antagonistic effects, and showed tolerance against the pesticides mixture. In fact, the growth of mixed cultures was significantly higher than in pure cultures. Moreover a pure culture (Streptomyces sp. A5) and a quadruple culture had the highest pentachlorophenol removal percentages (10.6 and 10.1%, respectively), while Streptomyces sp. M7 presented the best chlorpyrifos removal (99.2%). Mixed culture of all Streptomyces spp. when assayed either as free or immobilized cells, showed chlorpyrifos removal percentages of 40.17% and 71.05% respectively, and for pentachlorophenol 5.24% and 14.72% respectively, suggesting better removal of both pesticides by using immobilized cells. These results reveal that environments contaminated with mixtures of xenobiotics could be successfully cleaned up by using either free or immobilized cultures of Streptomyces, through in situ or ex situ remediation techniques.","palabras_clave":"STREPTOMYCES|PESTICIDES MIXTURE REMOVAL|PENTACHLOROPHENOL |CHLORPYRIFOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63423,"idioma_id":5,"titulo":"Fuera de programa. La ausencia de los sectores vulnerables en la formación jurídica","resumen":"El artículo aborda la problemática de la inserción en la formación profesional de los abogados de temáticas vinculadas con sectores vulnerables y su relación con el Derecho, en particular en lo que hace al sector campesino, la niñez y adolescencia y los jóvenes de sectores empobrecidos y marginados. Sobre la base del análisis de la bibliografía, de los programas de la carrera y de entrevistas a docentes y estudiantes avanzados, se advierte una inclusión insuficiente de estas temáticas tanto desde el punto de vista formal, en los programas de estudio, como en su abordaje efectivo durante el desarrollo de las respectivas asignaturas. Las conclusiones, en términos de los déficits que implican en la formación de profesionales comprometidos con la realidad, constituyen una invitación a reflexionar respecto de qué conocimiento generamos y transmitimos, quiénes son los destinatarios directos e indirectos de la educación universitaria y, en definitiva, qué tan dispuestos estamos a colaborar desde lo propio de la labor universitaria, con la construcción de una sociedad más justa.","palabras_clave":"SECTORES VULNERABLES|FORMACION JURIDICA|DERECHO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63424,"idioma_id":4,"titulo":"A Differential Redox Regulation of the Pathways Metabolizing Glyceraldehyde-3-Phosphate Tunes the Production of Reducing Power in the Cytosol of Plant Cells","resumen":"Abstract: Adaptation to aerobic life leads organisms to sense reactive oxygen species and use the signal for coordination of the entire metabolism. Glycolysis in plants is a particular network where specific steps, like oxidation of glyceraldehydes-3-phosphate (Ga3P), are critical in order for it to function. The triose-phosphate can be converted into 3-phosphoglycerate through the phosphorylating Ga3P dehydrogenase (Ga3PDHase, EC 1.2.1.12) producing ATP and NADH, or via the non-phosphorylating enzyme (np-Ga3PDHase; EC 1.2.1.9) generating NADPH. In this work we found redox regulation to be a posttranslational mechanism allowing the fine-tuning of the triose-phosphate fate. Both enzymes were inactivated after oxidation by reactive oxygen and nitrogen species. Kinetic studies determined that Ga3PDHase is marked (63-fold) more sensitive to oxidants than np-Ga3PDHase. Thioredoxin-h reverted the oxidation of both enzymes (although with differences between them), suggesting a physiological redox regulation. The results support a metabolic scenario where the cytosolic triose-phosphate dehydrogenases are regulated under changeable redox conditions. This would allow coordinate production of NADPH or ATP through glycolysis, with oxidative signals triggering reducing power synthesis in the cytosol. The NADPH increment would favor antioxidant responses to cope with the oxidative situation, while the thioredoxin system would positively feedback NADPH production by maintaining np-Ga3PDHase at its reduced active state.","palabras_clave":"GLYCOLYSIS|REDOX|REDUCING POWER|TRIOSE-PHOSPHATE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63427,"idioma_id":4,"titulo":"The effect of an invading filter-feeding bivalve on a phytoplankton assemblage from the Paraná system: a mesocosm experiment","resumen":"The effect of different concentrations of the bivalve Limnoperna fortunei on the algal density and structure and its filtration rate was estimated in a mesocosm experiment. The experiment was carried out using containers of 200L and three treatments: Control (without bivalves), C1 (with 128 individual bivalves), and C2 (with 256 individual bivalves). Decrease in phytoplankton density was detected in bivalve treatments after 6 h. A decrease of 52% and 86% of the initial density was measured in C1 and C2, respectively, after 48 h. All algae groups showed substantial decrease in densities (460%) and the composition of the algae assemblages changed in the presence of the bivalve. In particular, there was an increase in the density of Flagellates. The maximum estimated bivalve filtration rate was 357mLind_1 h_1 at 28_C. Our results suggest that the invading bivalves could significantly affect both the plankton density and assemblage structure in natural systems.","palabras_clave":"MUSSEL|INVASIVE|LIMNOPERNA FORTUNEI|PHYTOPLANKTON|SPECIES SELECTIVITI|FILTRATION RATE|MESOCOSM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63428,"idioma_id":4,"titulo":"Gasteroid mycobiota (Basidiomycota) from Polylepis australis woodlands of central Argentina","resumen":"Gasteroid fungi associated with Polylepis australis forests from Córdoba Mountain range, central Argentina, were studied. Samples were collected during April-May 2007. Twenty-nine species were identified among which Bovista pila, Geastrum morganii and Tulostoma xerophilum are new records for Argentina and the distribution range of Bovista nigrescens, Cyathus olla, Geastrum fornicatum and Lycoperdon pyriforme are expanded to high altitude woodlands from Córdoba.        download page     http:\/\/www.mycotaxon.com\/resources\/weblists.html","palabras_clave":"estrellas de tierra|diversidad fungica en bosques de altura|falos hediondos|hongos polvera"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63429,"idioma_id":4,"titulo":"Phylogeography and diversification of truffles in the Tuberaceae and identification of its sister lineage in the Southern Hemisphere","resumen":"Truffles have evolved from epigeous (aboveground) ancestors in nearly every major lineage of fleshy fungi. Because  accelerated rates of morphological evolution accompany the transition to the truffle form, closely related epigeous  ancestors remain unknown for most truffle lineages. This is the case for the quintessential truffle genus Tuber, which  includes species with socio-economic importance and esteemed culinary attributes. Ecologically, Tuber spp. form obligate  mycorrhizal symbioses with diverse species of plant hosts including pines, oaks, poplars, orchids, and commercially  important trees such as hazelnut and pecan. Unfortunately, limited geographic sampling and inconclusive phylogenetic  relationships have obscured our understanding of their origin, biogeography, and diversification. To address this problem,  we present a global sampling of Tuberaceae based on DNA sequence data from four loci for phylogenetic inference and  molecular dating. Our well-resolved Tuberaceae phylogeny shows high levels of regional and continental endemism. We  also identify a previously unknown epigeous member of the Tuberaceae \u2013 the South American cup-fungus Nothojafnea  thaxteri (E.K. Cash) Gamund&#305;´. Phylogenetic resolution was further improved through the inclusion of a previously  unrecognized Southern hemisphere sister group of the Tuberaceae. This morphologically diverse assemblage of species  includes truffle (e.g. Gymnohydnotrya spp.) and non-truffle forms that are endemic to Australia and South America. Southern  hemisphere taxa appear to have diverged more recently than the Northern hemisphere lineages. Our analysis of the  Tuberaceae suggests that Tuber evolved from an epigeous ancestor. Molecular dating estimates Tuberaceae divergence in  the late Jurassic (,156 million years ago), with subsequent radiations in the Cretaceous and Paleogene. Intra-continental  diversification, limited long-distance dispersal, and ecological adaptations help to explain patterns of truffle evolution and  biodiversity.","palabras_clave":"hongos gasteroides hipogeos|filogenia|Tuberaceae|estudios moleculares"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63430,"idioma_id":4,"titulo":"The conflict between agricultural expansion and priority conservation areas: Making the right decisions before it is too late","resumen":"Agricultural expansion generates problems to ecosystems and hinders conservation efforts. Although several researchers worldwide are working to develop efficient approaches for conserving biodiversity ade- 29  quately, certain decisions should be taken urgently. Here, by using two entire classes of vertebrates(amphibians and reptiles), we calculated optimal conservation area networks (CANs) under nine different scenarios (including three different degrees of land-use and three different degrees of ambition in conservation objectives), in a highly deforested province of central Argentina. We show that if conservation decisions are delayed further, not only will more land be necessary to meet a given conservation objective, but also that optimal CANs will have to have a much greater perimeter, a larger number of fragments and a less appropriate shape. Therefore, as agricultural frontiers continue to advance without designating specific sites for conservation, we are losing the possibility to conserve biodiversity adequately.","palabras_clave":"DESFORESTATION|CEONSERVATIOS AREAS|AMPHIBIANS|REPTILES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63431,"idioma_id":4,"titulo":"Monitoring and modeling land surface dynamics in Bermejo River Basin, Argentina: time series analysis of MODIS NDVI data","resumen":"The purpose of this work was to monitor and model land surface phenology over the pastten years in the South American Bermejo River basin using the Moderate ResolutionImaging Spectroradiometer (MODIS) normalized difference vegetation index (NDVI)product. In order to do this, we evaluated the characteristics of the satellite data andinformation available on the study area\u2019s ecosystem to choose the best model to capturethe temporal dynamics of NDVI in local vegetation (sufficiently complex to provide agood fit and simple enough so that each parameter has an immediate ecological meaning).An ecological interpretation of model parameters was provided. Different landsurfaces showed distinct fluctuations over time in NDVI values, and this informationwas used to improve object-oriented classification. A decision tree classification wasdeveloped to identify spatial patterns of NDVI functional form and the fluctuationsthat these patterns presented from 2000 to 2010. We integrated inter-annual informationin a final map that distinguishes stable areas from changing sites. Assuming thatlarge inter-annual spatial-scale fluctuations were related to climatic events, we establishedhow vegetated land surfaces within the study area responded to these. Our studywas designed to emphasize the interpretation of the spatial and temporal scales of landsurface phenology.","palabras_clave":"NDVI|MODELLING|MULTITEMPORAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63432,"idioma_id":4,"titulo":"Growth, survival and peptidolytic activity of Lactobacillus plantarum I91 in a hard-cheese model","resumen":"In this work, we studied the growth, survival and peptidolytic activity of Lactobacillus plantarum I91 in a hard-cheese model consisting of a sterile extract of Reggianito cheese. To assess the influence of the primary starter and initial proteolysis level on these parameters, we prepared the extracts with cheeses that were produced using two different starter strains of Lactobacillus helveticus (Lh138 or Lh209) at three ripening times: 3, 90 and 180 days. The experimental extracts were inoculated with Lb. plantarum I91; the control extracts were not inoculated, and the blank extracts were heat-treated to inactivate enzymes and were not inoculated. All extracts were incubated at 34ºC for 21 days, and then the pH, microbiological counts and proteolysis profiles were determined. The basal proteolysis profiles in the extracts of young cheeses made with either strain tested were similar, but many differences between the proteolysis profiles of the extracts of the Lh138 and Lh209 cheeses were found when riper cheeses were used. The pH values in the blank and control extracts did not change, and no microbial growth was detected. In contrast, the pH value in experimental extracts decreased, and this decrease was more pronounced in extracts obtained from either of the young cheeses and from the Lh209 cheese at any stage of ripening. Lb. plantarum I91 grew up to 8 log during the first days of incubation in all of the extracts, but then the number of viable cells decreased, the extent of which depended on the starter strain and the age of the cheese used for the extract. The decrease in the counts of Lb. plantarum I91 was observed mainly in the extracts in which the pH had diminished the most. In addition, the extracts that best supported the viability of Lb. plantarum I91 during incubation had the highest free amino acids content. The effect of Lb. plantarum I91 on the proteolysis profile of the extracts was marginal. Significant changes in the content of free amino acids suggested that the catabolism of free amino acids by Lb. plantarum I91 prevailed in a weakly proteolysed medium, whereas the release of amino acids due to peptidolysis overcame their catabolism in a medium with high levels of free amino acids. Lb. plantarum I91 was able to use energy sources other than lactose to support its growth because equivalent numbers of cells were observed in extracts containing residual amounts of lactose and in lactose-depleted extracts. The contribution of Lb. plantarum I91 to hard-cooked cheese peptidolysis was negligible compared to that of the starter strain; however, its ability to transform amino acids is a promising feature of this strain.","palabras_clave":"LACTOBACILLUS PLANTARUM|STERILE CHEESE EXTRACT|HARD-COOKED CHEESE|PROTEOLYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63433,"idioma_id":4,"titulo":"Techniques for measuring aerosol attenuation using the Central Laser Facility at the Pierre Auger Observatory","resumen":"The Pierre Auger Observatory in Malargue, Argentina, is designed to study the properties of ultra-high energy cosmic rays with energies above 10^18 eV. It is a hybrid facility that employs a Fluorescence Detector to perform nearly calorimetric measurements of Extensive AirShower energies. To obtain reliable calorimetric information from the FD, the atmospheric conditions at the observatory need to be continuously monitored during data acquisition. In particular, light attenuation due to aerosols is an important atmospheric correction. The aerosol concentration is highly variable, so that the aerosol attenuation needs to be evaluated hourly. We use light from the Central Laser Facility, located near the center of the observatory site, having an optical signature comparable to that of the highest energy showers detected by the FD. This paper presents two procedures developed to retrieve the aerosol attenuation of fluorescence light from CLF laser shots. Cross checks between the two methods demonstrate that results from both analyses are compatible, and that the uncertainties are well understood. The measurements of the aerosol attenuation provided by the two procedures are currently used at the Pierre Auger Observatory to reconstruct air shower data.","palabras_clave":"Data analysis|Large detector systems for particle and astroparticle physics|Detector alignment and calibration methods (lasers, sources, particle-beams)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63434,"idioma_id":4,"titulo":"Influence of environmental factors on spawning of sciaenids from the Buenos Aires Coastal Zone, Argentina","resumen":"The fish community in coastal waters of the Buenos Aires Province is dominated by seven sciaenid species: Micropogonias furnieri, Cynoscion guatucupa, Macrodon ancylodon, Pogonias cromis, Umbrina canosai, Paralonchurus brasiliensis, and Menticirrhus americanus. The relation between sciaenid spawning females and environmental factors was examined in order to describe the spawning strategy of these species in Argentine coastal waters. Sciaenid species were classified into three groups according to the thermohaline range ofthe spawning areas: (a) estuarine spawners associated with the bottom salinity front comprised those species that spawned in the inner area of the estuary in accordance with the main horizontal salinity gradients at the bottom (M. furnieri, M. ancylodon, and P. chromis); (b) estuarine spawners not associated with the bottom salinity front comprised those species that spawned mainly in the middle of the estuary, in brackish water with salinities ranging from 24 to 30 (P. brasiliensis and M. americanus); and (c) marine spawners comprised those species that alwaysspawned in salt water (salinity values higher than 30) in the outer area of the Río de la Plata Estuary or El Rincón, in the southern part of Buenos Aires Province (C. guatucupa, U. canosai, and M. furnieri). Micropogonias furnieri, the most abundant sciaenid in Argentina that presents the highest latitudinal distribution, can behave as a marine or estuarine spawner depending on the environmental characteristics. This allows it to make better use of each ecosystem and thus increase its survival chances","palabras_clave":"SCIAENIDAE|SPAWNING|ENVIRONMENT|RIO DE LA PLATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63435,"idioma_id":4,"titulo":"The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae","resumen":"Morphometrical and histological techniques were employed to characterize Pagrus pagrus larvae nutritional condition. Larvae were reared in laboratory under controlled conditions with the main objective of testing whether these methodologies allowed finding differences between larvae from different feeding treatments. Once yolk was consumed (3 days after hatching) larvae were assigned to a feeding treatment: T1 starved during the whole experiment; T2 delayed feeding, starved during three days; T3 fed during the entire experiment. Algae (Nannochloropsis oculata) and rotifers (Brachionus plicatilis) were provided to larvae for feed treatments. Larvae were fixed daily; for morphometrical purposes in 5% formaldehyde solution, and in Bouin for histological sections. Results herein obtained showed that both methodologies are sensitive enough to distinguish larvae characterized by different nutritional condition obtained from the feeding treatments. Consequently, these methodologies could be employed in wild red porgy larvae or in other species commercially exploited in order to asses their nutritional condition. These techniques could also be employed to check larval quality obtained with aquaculture purposes in order to estimate the effects of changes in rearing protocols or kind of food supply and thus, to guaranty a higher survival of early developmental stages of reared larvae with aquaculture purposes","palabras_clave":"CRITICAL PERIOD|STARVATION|LARVAL HISTOLOGY|RED PORGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63436,"idioma_id":5,"titulo":"LA SECUENCIA NEOPALEOZOICA DE LA QUEBRADA DE AGUA DE JAGÜEL (PRECORDILLERA DE MENDOZA): EDAD Y REDEFINICIÓN ESTRATIGRÁFICA","resumen":"Se propone la denominación de Formación Cordón de Jagüel para la sucesión pérmica de areniscas, pelitas y escasos conglomerados que forman la ladera occidental del Cordón de Agua de Jagüel en la Precordillera de Mendoza. Estas rocas descansan en discordancia sobre metasedimentitas de la Formación Villavicencio y son separadas por falla de areniscas, pelitas y diamictitas pertenecientes a la Formación Agua de Jagüel (Pennsylvaniano). En la Formación Cordón de Jagüel se han identificadoseis asociaciones de facies sedimentarias. La asociación 1 está integrada mayormente por conglomerados polimícticos y areniscas gruesas de origen fluvial. El inicio de un ascenso relativo del nivel del mar aparece registrado en la asociación de facies 2, formada por areniscas y pelitas carbonosas depositadas en ambientes transicionales, probablemente estuarinos. La asociación de facies 3, compuesta por areniscas finas, indica el pasaje a un ambiente marino costero, mientras que la asociación de facies 4 incluye areniscas con paquetes de estratificación entrecruzada de gran escala y probablemente corresponde a ambientes subtidales con importante crecimiento de barras de arena de gran porte. Una progresiva somerización llevó a la depositación de pelitas carbonosas, areniscas finas e intercalaciones de margas (asociación de facies 5) en ambientes restringidos (albuferas).Finalmente, la asociación de facies 6 (pelitas laminadas y areniscas finas) indica un nuevo evento transgresivo. Restos de invertebrados marinos pertenecientes a la Biozona de Costatumulus y de palinofloras conteniendo Lueckisporites virkkiae Potonié yKlaus sugieren una edad pérmica temprana, probablemente cisuraliana tardía para la unidad.","palabras_clave":"Pérmico temprano|Formación Cordón de jaguel|Precordillera|Gondwana"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63437,"idioma_id":4,"titulo":"In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies","resumen":"Abstract: The development of effi cient, rapid and species-specifi c techniques is indispensable for assessing growth and neutral lipid yield in microalga cultures for biodiesel oil production.  Nannochloropsis oculata is a small microalgae with a thick cell wall. In vivo techniques to estimate cell density, chlorophyll a and neutral lipids are reported. A calibration curve of cell  density versus optical density was obtained and validated at 540 nm, under different growth phases. Intracellular neutral lipid storage was evaluated with fl uorometry and epifl uorescent  microscopy employing fl uorochrome Nile Red. The addition of 5 % dimethyl sulfoxide enhanced 12.5 times the fl uorescence signal effi ciency. In situ fl uorescence measurements allowed estimating the neutral lipid content (NR-FI). Besides, no signifi cant differences were  found in the lipid neutral content between gravimetric and triolein methods. The relationship between NR-FI and chlorophyll fl uorescence signals was used as a neutral lipid accumulation index, which is useful in order to establish the optimum harvesting time. Thus, these procedures  may be applied for a better monitoring mode of growth and neutral lipid accumulation in N. oculata?s cultures at commercial scale.","palabras_clave":"NANNOCHLOROPSIS|NEUTRAL LIPIDS|CHLOROPHYLL A|OPTICAL DENSITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63438,"idioma_id":4,"titulo":"Constraints on the origin of cosmic rays above $10^{18}$ eV from large scale anisotropy searches in data of the Pierre Auger Observatory","resumen":"A thorough search for large-scale anisotropies in the distribution of arrival directions of cosmic rays detectedabove 1018 eV at the Pierre Auger Observatory is reported. For the first time, these large-scale anisotropy searchesare performed as a function of both the right ascension and the declination and expressed in terms of dipole andquadrupole moments.Within the systematic uncertainties, no significant deviation from isotropy is revealed. Upperlimits on dipole and quadrupole amplitudes are derived under the hypothesis that any cosmic ray anisotropy isdominated by such moments in this energy range. These upper limits provide constraints on the production ofcosmic rays above 1018 eV, since they allow us to challenge an origin from stationary galactic sources denselydistributed in the galactic disk and emitting predominantly light particles in all directions.","palabras_clave":"ASTROPARTICLE PHYSICS|COSMIC RAYS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63439,"idioma_id":4,"titulo":"The Interpretation of the Depths of  Shower Maximum of Extensive Air Showers Measured by the Pierre Auger Observatory","resumen":"To interpret the mean depth of cosmic ray air shower maximum and its dispersion,we parametrize those two observables as functions of the rst two moments of the lnAdistribution. We examine the goodness of this simple method through simulations of testmass distributions. The application of the parameterization to Pierre Auger Observatorydata allows one to study the energy dependence of the mean lnA and of its variance underthe assumption of selected hadronic interaction models. We discuss possible implications ofthese dependences in term of interaction models and astrophysical cosmic ray sources","palabras_clave":"ultra high energy cosmic rays|cosmic ray experiments"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63440,"idioma_id":4,"titulo":"Bounds on the density of sources of ultra-high energy cosmic rays from the Pierre Auger Observatory","resumen":"We derive lower bounds on the density of sources of ultra-high energy cosmic  rays from the lack of significant clustering in the arrival directions of the  highest energy events detected at the Pierre Auger Observatory. The density of  uniformly distributed sources of equal intrinsic intensity was found to be  larger than $sim (0.06 - 5) \times 10^{-4}$ Mpc$^{-3}$ at 95% CL, depending on  the magnitude of the magnetic deflections. Similar bounds, in the range $(0.2 -  7) \times 10^{-4}$ Mpc$^{-3}$, were obtained for sources following the local  matter distribution.","palabras_clave":"ultra high energy cosmic rays|cosmic ray experiments"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63441,"idioma_id":5,"titulo":"El infarto agudo de miocardio. El camino hacia nuevas terapias para  su tratamiento.","resumen":"El infarto agudo de miocardio constituye una urgencia médica que requiere atención inmediata.  Paradójicamente, algunas de las terapias más exitosas para su tratamiento pueden generar  una nueva lesión, similar a la original. ¿Cuáles son los mecanismos responsables de estas  complicaciones? ¿Cómo se puede avanzar en el diseño de nuevas terapias?","palabras_clave":"ISQUEMIA|CORAZÓN|TERAPIAS|MITOCONDRIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63442,"idioma_id":4,"titulo":"Phenomenology involved in self-pressurized, natural circulation, low thermo-dynamic quality, nuclear reactors: The thermal-hydraulics of the CAREM-25 reactor","resumen":"The interwoven phenomena involved in a prototypical self-pressurized natural circulation,low thermo-dynamical quality nuclear reactor such as CAREM-25 are analytically presented. These phenomena present many differences with traditional light water nuclear power plants. The dependence between mass flow and core inlet enthalpy on generated power is found. The need of tuning the mass flow rate in accordance to the design value is found to be important in order to keep the thermal margin and the heat transfer coefficients in the steam generators. The influence of condensation in structures or walls in the upper dome on the two-phase boundary is also studied. The dynamic consequences of all these results are therefore discussed. A numerical code is then used to verify the aforementioned findings and to test the validity of the modeling approximations. From the results it is clear that the way the phenomena interact causes the resulting dynamics in CAREM-25 to be substantially different from that existing in reactors such as PWRs, BWRs and also natural circulation BWRs. It is thus clear that the combination of different effects makes CAREM-25 behavior impossible to be extrapolated from existing knowledge and accumulated experience. For this reason an exhaustive analysis regarding the thermal-hydraulic performance (including stability analysis) needs to be performed.","palabras_clave":"NUCLEAR REACTOR|NATURAL CIRCULATION|THERMAL-HYDRAULICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63443,"idioma_id":4,"titulo":"Quantum dynamical simulations of local field enhancement in metal nanoparticles","resumen":"Field enhancements  around small Ag nanoparticles (NPs) are calculated using a quantum dynamical simulation formalism and the results are compared with electrodynamic simulations using the discrete dipole approximation (DDA) in order to address the important issue of the intrinsic atomistic structure of NPs. Quite remarkably, in both quantum and classical approaches the highest values of &#1113088; are located in the same regions around single NPs. However, by introducing a complete atomistic description of the metallic NPs in optical simulations, a different pattern of the &#1113088; distribution is obtained. Knowing the correct pattern of the &#1113088; distribution around NPs is crucial for understanding the spectroscopic features of molecules inside hot spots. The enhancement produced by surface plasmon coupling is studied by using both approaches in NP dimers for different inter-particle distances. The results show that the trend of the variation of &#1113088; versus inter-particle distance is different for classical and quantum simulations. This difference is explained in terms of a charge transfer mechanism that cannot be obtained with classical electrodynamics. Finally, time dependent distribution of the enhancement factor is simulated by introducing a time dependent field perturbation into the Hamiltonian, allowing an assessment of the localized surface plasmon resonance quantum dynamics.","palabras_clave":"metal nanoparticles|field enhancement|dda|quantum dynamics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63444,"idioma_id":4,"titulo":"The Structure, Energy, Confinement, and Enhancement of Hot Spots between Two Nanoparticles","resumen":"Hot spots (HSs) between two nanoparticles (NPs) can be considered the   canonical example of plasmonic coupling effects and are able to achieve   huge enhancements of signals from molecules in fluorescence and Raman   spectroscopy. In this work, it is found that these HSs between two NPs   can be modeled as a superposition of sub-HSs that are located on the   surface of each NP of the pair, a fact that can be important to describe   plasmon coupling effects. The analyzed HSs are almost fully   characterized by means of their energy, degree of localization, and mean   enhancement, quantities that are able to give a measure of their   performance. It is demonstrated that the interplay between the energy   and degree of localization is what actually determines the enhancement   in a general way, that is, HSs with similar energy could have a   large\/small enhancement depending on their degree of localization","palabras_clave":"HOT SPOT|ENHANCEMENT |NANOPARTICLE|PLASMON"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63445,"idioma_id":4,"titulo":"Semen quality and sperm function loss by hypercholesterolemic diet was recovered by addition of olive oil to diet in rabbit","resumen":"Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase in serum and sperm membrane chol, which ultimately altered membrane-coupled sperm specific functions: osmotic resistance, acrosomal reaction, and sperm capacitation in White New Zealand rabbits. These changes were also associated with a reduction in motility percentage and appearance of abnormal sperm morphology. The present study was carried out to evaluate the effect of dietary olive oil (OO, 7% v\/w) administration to several male hypercholesterolemic rabbits hypercholesterolemic rabbits, HCR) with altered fertility parameters. These HCR males were achieved by feeding normal rabbits with a high-fat diet (0.05%chol). HCR were associated with a modest non-significant increase in body weight (standard diet, 4.0860.17 Kg, versus high-fat diet, 4.3760.24 Kg). Hypercholesterolemic rabbits presented a marked decrease in semen volume, sperm cell count, and percentage of sperm motility, associated with a significant increase in sperm cell abnormalities. Moreover, sperm capacitation measured by the characteristic phosphorylated protein pattern in and induced acrosomal reaction were also altered suggesting sperm dysfunction. However, the administration of OO (for 16 weeks) to rabbits that were fed with 50% of the high-fat diet normalized serum chol. Curiously, OO supply succeeded to attenuate the seminal and sperm alterations observed in HCR group. Administration of OO alone did not cause any significant changes in above mentioned parameters. These data suggest that OO administration to HCR male rabbits recovers the loss of semen quality and sperm functionality.","palabras_clave":"Hypercholesterolemia|Olive oil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63446,"idioma_id":4,"titulo":"Shifts in d15N signature following the onset of exogenous feeding in fishes: Importance of combining length and age data","resumen":"The d15N isotopic change of recently emerged rainbow trout (Oncorhynchus mykiss) due to diet shift from yolk sac to exogenous feeding was evaluated in a field study. The fit of a general model including both fish length and age in days as co-variables indicates that the collection of isotope signatures could have arisen from a dilution process of a common maternally inherited signature. The results suggest that the specific d15N of individual fish at any given&nbsp; time along the ontogeny is determined by its growth trajectory and not by time or size alone. The results are discussed in the context of the potential bias in determining maternal origin in partially migratory salmonids through muscle isotope ratios based on fish size alone.","palabras_clave":"MATERNAL ORIGIN|PARTIAL ANADROMY|SALMONIDS|STABLE ISOTOPES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63447,"idioma_id":4,"titulo":"Genetic structure and different color morphotypes suggest the occurrence and bathymetric segregation of two incipient species of Sebastes off Argentina","resumen":"Rockfishes of the genus Sebastes are extensively distributed in the Pacific and Atlantic oceans. Although the occurrence of two morphologically similar species in the Southern Hemisphere, Sebastes oculatus and Sebastes capensis, is now clearly established, the taxonomic status and phylogeographic patterns for the genus in the region have not yet been completely resolved. In this study, we provide new insights into the taxonomy and evolutionary relationships of rockfishes inhabiting the Southwestern Atlantic Ocean, off the coast of mainland Argentina, by combining mitochondrial DNA (mtDNA) control region sequences, microsatellite data and color pattern analyses. Differences in coloration (-dark- and -light- fish) together with bathymetric segregation between color morphotypes were evident from fish collection and literature review. In addition, the mtDNA phylogenetic analysis and Bayesian clustering analysis using microsatellite data separated the fish into two distinct groups (FST = 0.041), most likely representing incipient species. Our results suggest that speciation-by-depth in the absence of physical barriers could be a widespread mechanism of speciation in Sebastes from both the Northern and Southern Hemispheres. Nevertheless, the degree of genetic differentiation found, added to the large number of individuals displaying high levels of admixture, points to the occurrence of incomplete reproductive barriers between color morphotypes. Beyond the taxonomic and phylogeographic implications of our findings, the occurrence of distinct groups of Sebastes off the coast of Argentina being targeted by different fisheries (angling and trawling) have consequences for the design and implementation of appropriate fishery regulations to avoid overharvest of either group.","palabras_clave":"SEBASTIDAE|PHYLOGEOGRAPHY|ROCKY REEF FISH|INCOMPLETE REPRODUCTIVE ISOLATION|INCIPIENT SPECIATION|MORPHOTYPES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63448,"idioma_id":5,"titulo":"Influencia del tamaño de las partículas del agregado reactivo frente a los álcalis en las barras de mortero","resumen":"Uno de los temas más investigados vinculado a la durabilidad de las estructuras de hormigón es la reacción álcali-sílice (RAS), ya que esta reacción se produce dentro del hormigón y provoca fallas estructurales de gran importancia cuando no es controlada. Para que se produzca necesitamos un agregado potencialmente reactivo, álcalis (cemento portland) y humedad. La reacción produce un gel silico-alcalino expansivo que genera fisuras y deterioro de las estructuras de hormigón. Otrosfactores que controlan la RAS son el porcentaje y el tamaño de partícula de agregado reactivo,&nbsp; que pueden generar un efecto de ?pessimum? produciendo valores de máxima expansión en morteros u hormigones. En el presente trabajo se tratará de establecer, mediante el ensayo&nbsp; acelerado de la barra de mortero, si el efecto de ?pessimum? se hace efectivo a un tamaño de partícula específico de agregado reactivo. En la confección de los morteros se utilizó un agregado fino compuesto por 90% de arena natural sílico-feldespática no reactiva y un 10% de arenisca ?de Chaco? (arena de trituración) potencialmente reactiva, tomando de está última diferentes fracciones granulométricas para la realización de las distintas mezclas. Los resultados del ensayo físico y el análisis petrográfico de las barras muestran que los mayores valores de expansión coinciden con un rango granulométrico determinado y estos resultados se corroboran por la presencia de los rasgos deletéreos observados durante el estudio petrográfico.","palabras_clave":"MORTEROS|ARENISCA|RAS|PESSIMUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63449,"idioma_id":4,"titulo":"Ritonavir-boosted lopinavir plus nucleoside or nucleotide reverse transcriptase inhibitors versus ritonavir-boosted lopinavir plus raltegravir for treatment of HIV-1 infection in adults with virological failure of a standard first-line ART regimen (SECOND","resumen":"Background Uncertainty exists about the best treatment for people with HIV-1 who have virological failure with  fi rst-line combination antiretroviral therapy of a non-nucleoside analogue (NNRTI) plus two nucleoside or nucleotide  analogue reverse transcriptase inhibitors (NtRTI). We compared a second-line regimen combining two new classes of  drug with a WHO-recommended regimen.  Methods   We did this 96-week, phase 3b\/4, randomised, open-label non-inferiority trial at 37 sites worldwide. Adults  with HIV-1 who had confi rmed virological failure (plasma viral load >500 copies per mL) after 24 weeks or more of  fi rst-line treatment were randomly assigned (1:1) to receive ritonavir-boosted lopinavir plus two or three NtRTIs  (control group) or ritonavir-boosted lopinavir plus raltegravir (raltegravir group). The randomisation sequence was  computer generated with block randomisation (block size four). Neither participants nor investigators were masked  to allocation. The primary endpoint was the proportion of participants with plasma viral load less than 200 copies  per mL at 48 weeks in the modifi ed intention-to-treat population, with a non-inferiority margin of 12%. This study is  registered with ClinicalTrials.gov, number NCT00931463.  Findings We enrolled 558 patients, of whom 541 (271 in the control group, 270 in the raltegravir group) were included  in the primary analysis. At 48 weeks, 219 (81%) patients in the control group compared with 223 (83%) in the  raltegravir group met the primary endpoint (diff erence 1·8%, 95% CI \u20134·7 to 8·3), fulfi lling the criterion for  non-inferiority. 993 adverse events occurred in 271 participants in the control group versus 895 in 270 participants in  the raltegravir group, the most common being gastrointestinal.  Interpretation The raltegravir regimen was no less effi cacious than the standard of care and was safe and well tolerated.  This simple NtRTI-free treatment strategy might extend the successful public health approach to management of HIV  by providing simple, easy to administer, eff ective, safe, and tolerable second-line combination antiretroviral therapy.  Funding University of New South Wales, Merck, AbbVie, the Foundation for AIDS Research.","palabras_clave":"Ritonavir-boosted lopinavir |nucleoside or nucleotide reverse transcriptase inhibitors|raltegravir"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63450,"idioma_id":4,"titulo":"Acridones as antiviral agents: synthesis, chemical and biological properties","resumen":"Acridones are a class of compounds that have attracted attention in recent years for their wide range of biological properties, including selective inhibition of diverse human pathogenic viruses. The wide spectrum of antiviral activity includes DNA and RNA viruses, such as herpes simplex virus, cytomegalovirus, adenovirus, hepatitis C virus, dengue virus, and Junin virus, among others, indicative of the involvement of cellular factors as potential targets of acridone derivatives. At the present, their precise mode of action is not clearly determined, although the predominant action seems to be centered on the synthesis of nucleic acids. Regarding this point, inhibitory activity against cellular and viral enzymes and the ability to intercalate into nucleic acid molecules was demonstrated for some acridone compounds. Then, the possibility of a multiple effect on different targets renewed interest in these agents for virus chemotherapy allowing a potent inhibitory effectiveness associated to less feasibility of generating antiviral resistance. This review summarizes the current knowledge regarding the methods of synthesis, the antiviral properties of acridone derivatives, their mechanism of action, and structural characteristics related to antiviral activity as well as the perspectives of this class of compounds for clinical application against human viral infections.","palabras_clave":"ACRIDONES|ANTIVIRAL|ARENAVIRUS|FLAVIVIRUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63451,"idioma_id":4,"titulo":"Toward Integrated Production and Distribution Management in Multi-Echelon Supply Chains","resumen":"The effective management of multi-site systems involves the proper coordination of activities performed in multiple factories, distribution centers (DCs), retailers and end-users located in many different cities, countries and\/or continents. To optimally manage numerous production and transportation decisions, a novel monolithic continuous-time MILP-based framework is developed to determine the best short-term operational planning to meet all customer requests at minimum total cost. The formulation lies on the unit-specific general precedence concept for the production scheduling problem whereas the immediate precedence notion is used for transportation decisions. To illustrate the applicability and potential benefits of the model, a challenging example corresponding to a supply chain comprising several locations geographically spread in six European countries has been solved to optimality with modest CPU times. Several scenarios with different logistics features were addressed in order to remark the significant advantages of using the integrated approach.","palabras_clave":"MILP-based approach|Production and Distribution Scheduling|Multi-site System|Logistics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63452,"idioma_id":4,"titulo":"Logistics Management in Maritime Transportation Systems","resumen":"The efficient routing and scheduling of multi-parcel chemical tankers is a challenging problem for both chemical and shipping industry. To optimally manage complex logistics problem, a novel continuous time precedence-based MILP mathematical formulation is developed to determine the optimal solution to the ship routing and scheduling. The approach aims to determine the assignment of cargos to ships and define the optimal route that each ship should follow to maximize its profit. The MILP-based model is then combined with an iterative algorithm in order to tackle large-scale problems involving a large numbers of ships, ports, and cargos. To illustrate the applicability and importance of the proposed method, a real industrial case study taken from literature is solved with modest CPU times.","palabras_clave":"Ship Routing and Scheduling|MILP-based Strategies|Heuristic Techniques|Maritime Transportation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63453,"idioma_id":4,"titulo":"Knowledge-based approach for the integration of the planning and scheduling decision making levels","resumen":"This work aims to integrate the tactical and operational decision making levels. A typical Scheduling mixed integer linear programming (MILP) model has been solved using several demand scenarios. The results have been analyzed and accordingly the operation cost vs. production levels nonlinear equations have been obtained. The aforementioned equations have been included as constraints of the SC planning problem. Hence, production, inventory and distribution variables along the complete SC have beenoptimized using a NLP model.","palabras_clave":"Knowledge-based Approach|Planning and Scheduling|NLP Model|Supply Chain"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63454,"idioma_id":4,"titulo":"Unique thermal behavior of sphingomyelin species with nonhydroxy and 2-hydroxy very-long-chain (C28-C32) polyunsaturated fatty acids","resumen":"In rat germ cells and spermatozoa, sphingomyelin (SM) contains molecular species with nonhydroxy (n) and 2-hydroxy (h) very-long-chain-polyunsaturated fatty acids (V), the most abundant being SMs with (n- and h-) 28:4n-6, 30:5n-6, and 32:5n-6 as acyl chains. The aim of this study was to gain information about their thermotropic behavior and interactions with other lipids. After isolation from rat testis, multilamellar and giant unilamellar vesicles from these SMs were examined using fluorescent probes. Only n-32:5 SM and h-32:5 SM displayed a gel-liquid transition temperature (Tt ¡Ö 21-22¡ãC), the rest remaining in the liquid state in the 5¡ãC-45¡ãC range. The degree of order was larger in bilayers of any of the h-V SMs than in those of their chain-matched n-V SMs. Both, but n-V SM relatively more than h-V SM, decreased the Tt of dimyristoylphosphatidylcholine as their proportion increased in binary PC:SM liposomes. In contrast to the established ability of 16:0-SM to form lateral cholesterol\/SM-rich ordered domains in ternary dioleoylphosphatidylcholine:cholesterol:SM bilayers, neither n-V SM nor h-V SM showed a tendency to do so. Thus, these SMs are in the fluid state and are not involved in this type of domains in spermatozoa at physiological temperatures. However, this state could be altered at the very low temperatures at which these gametes are usually preserved","palabras_clave":"2-HYDROXY FATTY ACIDS|ANISOTROPY|CHOLESTEROL-SPHINGOMYELIN DOMAINS|GENERALIZED POLARIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63455,"idioma_id":4,"titulo":"Central dopamine D2 receptors regulate growth hormone-dependent body growth and pheromone signaling to conspecific males.","resumen":"Competition between adult males for limited resources such as food and receptive females is shaped by the male pattern of pituitary growth hormone (GH) secretion that determines body size and the production of urinary pheromones involved in male-to-male aggression. In the brain, dopamine (DA) provides incentive salience to stimuli that predict the availability of food and sexual partners. Although the importance of the GH axis and central DA neurotransmission in social dominance and fitness is clearly appreciated, the two systems have always been studied unconnectedly. Here we conducted a cell-specific genetic dissection study in conditional mutant mice that selectively lack DA D2 receptors (D2R) from pituitary lactotropes (lacDrd2KO) or neurons (neuroDrd2KO). Whereas lacDrd2KO mice developed a normal GH axis, neuroDrd2KO mice displayed fewer somatotropes; reduced hypothalamic Ghrh expression, pituitary GH content, and serum IGF-I levels; and exhibited reduced body size and weight. As a consequence of a GH axis deficit, neuroDrd2KO adult males excreted low levels of major urinary proteins and their urine failed to promote aggression and territorial behavior in control male challengers, in contrast to the urine taken from control adult males. These findings reveal that central D2Rs mediate a neuroendocrine-exocrine cascade that controls the maturation of the GH axis and downstream signals that are critical for fitness, social dominance, and competition between adult males.","palabras_clave":"DOPAMINE|GROWTH HORMONE|BEHAVIOR|MUP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63456,"idioma_id":4,"titulo":"Application of LnCrTeO6 oxides as new ceramic pigments of the type 'green chromium'","resumen":"The family of green mixed oxides LnCrTeO6 (Ln = La, Nd, Gd, Dy, Er, Tm   and Yb) was investigated as potential new green ceramic pigments. Prior   to enamelling, they were fully characterized by XRD analysis and FTIR   spectroscopy. The materials were tested by enamelling with transparent   and opaque glazes, applied for single and double firing. The chromatic   qualities of all the formulations, obtained in the thermal range   850-1000 &#61616;C, were evaluated. The colour performance is fairly maintained   at 1000 &#61616;C, in the transparent frit, and a lighter green hue is achieved   in the opaque frit. In presence of a PbO-containing frit, a dark green   yellowish shade is obtained. The colorimetric parameters a and b are   within the range of typical pigments based on Cr2O3 in silicate and   aluminate glazes. The homogeneity of the enamels was confirmed by   SEM-EDX microscopy. These materials are promising for ceramic   applications since a low amount of Cr-pigment would be necessary to   achieve intense green coloration.","palabras_clave":"LnCrTeO6 OXIDES|Cr-CERAMIC GLAZES|XRD ANALYSIS|FTIR SPECTROSCOPY|COLOUR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63457,"idioma_id":4,"titulo":"The Chacoan bat fauna identity: patterns of distributional congruence and conservation implications","resumen":"The aim of this paper is to characterize the Chaco thorn-scrub savanna as a unit, biologically defined by means of its identification as a regional pattern of distributional congruence, through the evaluation of the utility of bat assemblages as biogeographical indicators of the Chaco at regional scale, and to then evaluate this characterization in terms of the conservation value of this habitat. We have considered the whole territory of Paraguay and Bolivia, and the northern of Argentina; that is the entire Chaco biogeographic province, and part of the surrounding biogeographic provinces. We conducted an exhaustive search and constructed a database consisting in 9509 geo-referenced records showing that the bat fauna of the Chaco is richer than previously reported. We used an optimality criterion to identify patterns of distributional congruence determined by the distribution of bats. We recovered several areas that correspond with the Chaco. The areas that are coincident with the Chaco were supported by eight species: Eptesicus brasiliensis, E. diminutus, Eumops dabbenei, Histiotus velatus, Promops centralis, P. nasutus, Pygoderma bilabiatum, and Tonatia bidens. These results show that bat assemblages are useful as characterizing of regional patterns of distributional congruence. These patterns provide first-step testable hypotheses of areas of endemism for future analyses of neighboring regions or analyses at more inclusive scales. Bat assemblages may be used to characterize the Chaco despite the fact that it was thought this habitat lacked an identity in relation to its bat fauna. The Chacoan nature as a biologically distinctive natural unit is clear now also in terms of regional patterns of distributional congruence of bat species, which provides further evidence for intensifying efforts to protect this endangered habitat.","palabras_clave":"Chaco|Chiroptera|Biogeography|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63458,"idioma_id":4,"titulo":"The Developmental Brain Gene NPAS3 Contains the Largest Number of Accelerated Regulatory Sequences in the Human Genome","resumen":"To identify the evolutionary genetic novelties that contributed to shape human-specific traits such as the use of a complex language, long term planning and exceptional learning abilities is one of the ultimate frontiers of modern biology. Evolutionary signatures of functional shifts could be detected by comparing non-coding regions that are highly conserved across mammals or primates and rapidly accumulated nucleotide substitutions only in the lineage leading to humans. Since gene loci densely populated with human accelerated elements (HAEs) are more likely to have contributed to human-specific novelties we sought to identify the transcriptional units and genomic 1 Mb intervals of the entire human genome carrying the highest number of HAEs. To this end we took advantage of four available datasets of human genomic accelerated regions obtained through different comparisons and algorithms and performed a meta-analysis of the combined data. We found that the brain developmental transcription factor NPAS3 contains the largest cluster of non-coding accelerated regions in the human genome with up to 14 elements that are highly conserved in mammals, including primates, but carry human-specific nucleotide substitutions. We then tested the ability of the 14 HAEs identified at the NPAS3 locus to act as transcriptional regulatory sequences in a reporter expression assay performed in transgenic zebrafish. We found that 11 out of the 14 HAEs present in NPAS3 act as transcriptional enhancers during development, particularly within the nervous system. Since NPAS3 is known to play a crucial role during mammalian brain development, our results indicate that the high density of HAEs present in the human NPAS3 locus could have modified the spatio-temporal expression pattern of NPAS3 in the developing human brain and, therefore, contributed to human brain evolution.","palabras_clave":"brain|human|evolution|NPAS3"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63459,"idioma_id":4,"titulo":"Age-of-onset of menopause is associated with enhanced painful and non-painful sensitivity in fibromyalgia","resumen":"Age-of-onset of menopause is associated with enhanced painful and non-painful sensitivity in fibromyalgia                                   Martinez-Jauand M, Sitges C, Femenia J, Cifre I, Gonzalez S, Chialvo D, Montoya P.           Martinez-Jauand M, Sitges C, Femenia J, Cifre I, Gonzalez S, Chialvo D, Montoya P.       Fibromyalgia (FM) is a chronic pain condition characterized by high prevalence in women. In particular, estrogen deficit has been considered as a potentially promoting factor of FM symptoms. This study was aimed to examine the relationship between age-of-onset of menopause and pain sensitivity in FM. For this purpose, pain sensitivity was assessed in 74 FM and 32 pain-free control women. All participants were postmenopausal and underwent a detailed semi-structured clinical interview, including data about menopause transition, previous history of hysterectomy or ovariectomy, and menses time. Participants were divided into two groups depending on age-of-onset of menopause: early menopause [&lt; 49 years] vs. late menopause [&gt;49&nbsp;years]. Pain and non-pain thresholds were assessed by using cold, heat, mechanical, and electrical stimulation. FM women showed higher overall pain sensitivity as compared with healthy subjects. FM women with early age-of-onset of menopause displayed greater pain and non-pain sensitivity than FM women with late age-of-onset of menopause, whereas no differences were observed in healthy women due to age-of-onset of menopause. These results suggest that an early transition to menopause (shortening the time of exposure to estrogens) may influence pain hypersensitivity and could be related to aggravation of FM symptoms.                         ","palabras_clave":"FIBROMYALGIA|MENOPAUSE|NON-PAIN THRESHOLDS|ESTROGEN|PAIN THRESHOLDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63460,"idioma_id":4,"titulo":"Senecio pseudodepressus (Asteraceae, Senecioneae), a New Species from San Juan Province, Argentina","resumen":"Senecio pseudodepressus, a new species from the Province of San Juan, Argentina, is described and illustrated. Senecio pseudodepressus is morphologically similar to Senecio depressus, but it is distinguished by having mostly 3-8-dentate leaves, pedunculate capitula and pubescent achenes. The new species is assigned to Senecio section Senecio series Chilenses subseries Candidi by its subshrubby habit, tomentose indumentum and solitary discoid capitula. A key to distinguish species of Senecio subser. Candidi with dentate leaves and large involucres is provided. The differences between Senecio pseudodepressus and its related taxa are also discussed.","palabras_clave":"Compositae|Senecio ser. Chilenses|Senecio subser. Candidi| taxonomy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63461,"idioma_id":4,"titulo":"Minute Time Scale Prolyl Isomerization Governs Antibody Recognition of an Intrinsically Disordered Immunodominant Epitope","resumen":"Conformational rearrangements in antibodyantigen recognitionare essential events where kinetic discrimination of isomersexpands the universe of combinations.Weinvestigated theinteraction mechanism of a monoclonal antibody, M1, raisedagainst E7 from human papillomavirus, a prototypic viral oncoproteinand a model intrinsically disordered protein. Themapped 12-amino acid immunodominant epitope lies within a?hinge? region between the N-terminal intrinsically disorderedand the C-terminal globular domains. Kinetic experimentsshow that despite being within an intrinsically disorderedregion, the hinge E7 epitope has at least two populations separatedby a high energy barrier. Nuclear magnetic resonancetraced the origin of this barrier to a very slow (t1&#8260;24 min) transcisprolyl isomerization event involving changes in secondarystructure. The less populated (10%) cis isomer is the bindingcompetentspecies, thus requiring the 90% of molecules in thetrans configuration to isomerize before binding. The associationrate for the cis isomer approaches 6107M1 s1, a ceilingfor antigen-antibody interactions. Mutagenesis experimentsshowed that Pro-41 in E7Ep was required for both binding andisomerization. After a slow postbinding unimolecular rearrangement,a consolidated complex with KD  1.2  107M isreached. Our results suggest that presentation of this viralepitope by the antigen-presenting cells would have to be?locked? in the cis conformation, in opposition to the most populatedtrans isomer, in order to select the specific antibody clonethat goes through affinity and kinetic maturation.","palabras_clave":"PROLYL ISOMERIZATION|INTRINSICALLY DISORDERED PROTEIN|ANTIBODY-ANTIGEN RECOGNITION|CONFORMATIONAL SELECTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63462,"idioma_id":4,"titulo":"Percolation of dimers on square lattices","resumen":"A theoretical approach, based on exact calculations of configurations on   finite rectangular cells, is applied to study the percolation of   homonuclear dimers on square lattices. An efficient algorithm allows us   to calculate the detailed structure of the configuration space for M= Lx× Ly   cells, with M varying from 16 to 36. The percolation process has been   monitored by following the percolation function, defined as the ratio   between the number of percolating configurations and the total number of   available configurations for a given cell size and concentration of   occupied sites. The percolation threshold has been calculated by means   of two complementary methods: one based on well-known renormalization   techniques and the other based on determining the inflection point of   the percolation function curves. A comparison of the results obtained by   these two methods has been performed. The study includes the use of   finite-size scaling theory to extrapolate numerical results towards the   thermodynamic limit. The effect of jamming due to dimers is also   established. Finally, the critical exponents &#957;, &#946; and &#947; have been   obtained and values compared with numerical results and expected   theoretical estimations. The present results show agreement and even   improvement (in the case of &#947;) with respect to some numeric values   available in the literature.","palabras_clave":"PERCOLATION|MULTISITE OCCUPANCY|CRITICAL EXPONENTS|SCALING PHENOMENA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63463,"idioma_id":4,"titulo":"Phase behavior of attractive k-mers on two-dimensional lattices: a study from quasi-chemical approximation","resumen":"The phase behavior of attractive linear rigid  k-mers on two-dimensional lattices was studied by theoretical  and simulation calculations in the framework of the  lattice-gas model. Combining (i) the analytical expression  for the partition function of non-interacting k-mers, and (ii)  a generalization of the classical quasi-chemical approximation  (QCA) in which the adsorbate can occupy more  than one adsorption site, the main thermodynamic functions  of the system were explicitly obtained. It was found  that, for temperatures below a certain condensation temperature,  the system undergoes a first-order phase transition  which is observed as a clear discontinuity in the adsorption  isotherms. The transition was studied in detail by calculating  the temperature\u2013density phase diagrams. Comparisons  with analytical data from Bragg\u2013Williams approximation  and Monte Carlo simulations were performed in order to  test the validity of the theoretical model. The results  obtained allowed us not only to analyze the effect of  introducing the lateral interactions by following the configuration-  counting procedure of the QCA, but also to  discuss the consequences of choosing the configurational  factor associated to adsorption of non-interacting k-mers  from different models developed to treat the multisite  occupancy adsorption problem.","palabras_clave":"Multisite occupancy adsorption| Phase transitions| Quasi-chemical approximation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63464,"idioma_id":4,"titulo":"Statistical thermodynamics of molecules with multiple adsorption states: application to protein adsorption","resumen":"The statistical thermodynamics of molecules with multiple adsorption states has been studied using a lattice model. The thermodynamic functions calculated for a system characterized by two adsorption states are applied to describe the adsorption of antifreeze proteins onto an ice crystal. The theoretical formalism reproduces the classical Langmuir equation (one-state limit), leads to the exact statistical thermodynamics  of molecules adsorbed in one dimension with n dierent adsorption states,  and provides a close approximation for two-dimensional systems. Comparisons with Monte Carlo simulations and analytical data derived from kinetic arguments are performed in order to test the validity of the theoretical model. The resulting framework represents a qualitative advance with respect to the existing development on multistate adsorption thermodynamics and seems to be a promising way toward a more accurate description of the adsorption thermodynamics of structurally diverse  proteins.","palabras_clave":"STATISTICAL THERMODYNAMICS|PROTEIN ADSORPTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63465,"idioma_id":4,"titulo":"Determination of volatile organic compounds of Tagetes filifolia Lag. (Asteraceae) from Córdoba (Argentina) using HS-SPME analysis","resumen":"A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC\u2013FID) are described for the analysis of volatile compounds in Tagetes filifolia Lag. The composition of the total aerial parts of the plant (whole plant) and the inflorescences, leaves and stems were studied separately using HS-SPME. As a result, 54 compounds were determined, of which 47 were identified. The major components observed in this analysis were trans-anethole and estragole. The HS-SPME method used for the analysis of volatile compounds of T. filifolia is simple, fast, effective, free from the use of solvents, and permits by an analysis of small amounts of samples to achieve better results in terms of the determination of the composition than those reported in the literature for the analysis of essential oil.","palabras_clave":"HS-SPME; | TAGETES FILIFOLIA LAG|VOLATILE|ORGANIC COMPOUNDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63466,"idioma_id":4,"titulo":"Spatial variability of chlorophyll-a and abiotic variables in a river-floodplain system during different hydrological phases","resumen":"Chlorophyll-a (Chl-a) and abiotic variables were measured in the main channel and floodplain waterbodies of the Middle Parana´ River to  analyse the system dynamics and to assess their spatial variability during different hydrological phases, including an extreme flood. We wanted to test that the flood does not always have a homogenising effect  in a river?floodplain system. An explanatory model for Chl-a was performed according to Akaike?s Information Criterion (AIC), and the relation of water level with the coefficient of variation (CV) among  sites for each variable was explored. The model explained 64% of Chl-a variability. Water level, depth:euphotic zone ratio (Zd:Zeu) (inverse correlation) and conductivity (direct correlation) were the significant explicative variables. The CV of Chla decreased with flood from the main channel to the floodplain, but for turbidity, Zd:Zeu, pH, dissolved  oxygen, soluble reactive phosphorus and Chla: pheophytin-a ratio, it increased. However, within the floodplain, CV of turbidity, Zd:Zeu and pH  decreased during flood. These suggest that the homogenising effect frequently observed during inundation cannot be generalised and that the floodplain may maintain its identity even during flood. The extreme flood and its overlap with the warm season and sedimentological pulse probably contributed to the heterogeneity in the spatial gradient.","palabras_clave":"PHYTOPLANKTONIC CHLOROPHYLL-A |LARGE FLOODPLAIN RIVER |SPATIAL HETEROGENEITY\/HOMOGENEITY |HYDROLOGICAL FLUCTUATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63467,"idioma_id":4,"titulo":"Ecomorphology of the African felid ensemble: The role of the skull and postcranium in determining species segregation and assembling history","resumen":"Morphology of extant felids is regarded as highly conservative. Most previous studies have focussed on skull morphology, so a vacuum exists about morphofunctional variation in postcranium and its role in structuring ensembles of felids in different continents. The African felid ensemble is particularly rich in ecologically specialized felids. We studied the ecomorphology of this ensemble using 31 cranial and 93 postcranial morphometric variables measured in 49 specimens of all 10 African species. We took a multivariate approach controlling for phylogeny, with and without body size correction. Postcranial and skull + postcranial analyses (but not skull-only analyses) allowed for a complete segregation of species in morphospace. Morphofunctional factors segregating species included body size, bite force, zeugopodial lengths and osteological features related to parasagittal leg movement. A general gradient of bodily proportions was recovered: lightly built, long-legged felids with small heads and weak bite forces vs. the opposite. Three loose groups were recognized: small terrestrial felids, mid-to-large sized scansorial felids and specialized Acinonyx jubatus and Leptailurus serval. As predicted from a previous study, the assembling of the African felid ensemble during the Plio-Pleistocene occurred by the arrival of distinct felid lineages that occupied then vacant areas of morphospace, later diversifying in the continent.","palabras_clave":"Africa|cranium|ecomorphology|ensemble|Felidae|postcranium|species segregation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63468,"idioma_id":4,"titulo":"The Placental Mammal Ancestor and the Post-K-Pg Radiation of Placentals","resumen":"To discover interordinal relationships of living and fossil placental mammals and the time of origin of placentals relative to the Cretaceous-Paleogene (K-Pg) boundary, we scored 4541 phenomic characters de novo for 86 fossil and living species. Combining these data with molecular sequences, we obtained a phylogenetic tree that, when calibrated with fossils, shows that crown clade Placentalia and placental orders originated after the K-Pg boundary. Many nodes discovered using molecular data are upheld, but phenomic signals overturn molecular signals toshow Sundatheria (Dermoptera + Scandentia) as the sister taxon of Primates, a close link between Proboscidea (elephants) and Sirenia (sea cows), and the monophyly of echolocating Chiroptera (bats). Our tree suggests that Placentalia first split into Xenarthra and Epitheria; extinct New World species are the oldest members of Afrotheria","palabras_clave":"Placental Mammals|K-PG"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63469,"idioma_id":4,"titulo":"Ultrasonographic and endocrine characterization of follicular waves in llamas with a special reference to the overlapping phenomenon during successive waves","resumen":"Ovarian follicular growth in non-mated llamas occurs in  successive waves that generally superimpose their origin on the  regression of the preceding wave (overlapping), originating  prolonged sexual receptivity in the species. The aim of this  study was to perform an ultrasonographic and endocrine  characterization of individual and successive waves in nonmated  llamas with a special interest on the overlapping  phenomenon. Twelve llamas were examined daily by transrectal  ultrasonography for at least two consecutive waves. In six  females, blood samples were collected daily at the end of each  examination. The development of the largest follicle (F)  showed a wavelike pattern with a mean duration of 25 days.  All waves evaluated were partially overlapped on the preceding  wave and emerged at a mean interval of 15.8  0.5 days.  This interwave interval determines a mean overlapping degree  of 32% of the wave length. Similarly, mean plasma oestradiol-  17b (E2) concentrations followed a wavelike pattern. However,  E2 concentrations started to decline before the structural  regression of the F was observed. Mean basal E2 concentrations  remain higher than 10.9  0.6 pmol\/l. In conclusion,  follicular activity in non-mated llamas is characterized by  continuous emergence of successive waves that always overlap  the preceding wave with variable degrees. E2 production  during the follicular wave is shorter in duration than the  morphological development of the F. Finally, the overlapping  phenomenon maintains increased plasma E2 concentrations  persistently and this could explain the prolonged periods of  sexual receptivity registered in llamas.","palabras_clave":"Follicular Activity|Oestradiol|Llamas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63470,"idioma_id":5,"titulo":"La ?necesidad sentida? del progreso. Construcción y representación del espacio urbano en Santa Fe. Fines del siglo XIX","resumen":"En este período de formación estatal, las atribuciones asumidas por las instituciones estatales tuvieron en la reconfiguración y formalización del aparato represivo una de sus manifestaciones más patentes. Durante la segunda mitad del siglo XIX, en la ciudad de Santa Fe, el control y el castigo de los comportamientos cotidianos en los espacios públicos estuvieron a cargo de la Policía, ella misma en conformación. Desde un enfoque crítico sobre las relaciones sociales de poder, emergen nuevas preguntas a realizar al respecto a las fuentes policiales.  En este sentido, la información que ellas brindan sobre los sujetos perseguidos y apresados por delitos contra el orden público arroja información plausible de utilizarse en análisis tanto cuantitativos como cualitativos, que contribuyan a reconstruir la conformación de los sectores subalternos urbanos como objeto de control, dada la notoria escasez de fuentes procedentes de dichos sectores.  El presente trabajo analiza partes emitidos por la Jefatura de Policía del Departamento La Capital al Poder Ejecutivo provincial, proponiendo dos líneas de indagación: qué información se brinda y cuál no sobre los sujetos arrestados (género, procedencia, edad, ocupación, entre otras) y qué apreciaciones subjetivas hacen los funcionarios policiales sobre dichos sujetos.","palabras_clave":"CARTOGRAFIA|ORDEN SOCIAL |REPRESENTACIONES SOCIALES|ESPACIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63471,"idioma_id":4,"titulo":"Behavior of the equivalent slab thickness over three European stations","resumen":"The total electron content (TEC) derived from the global positioning system (GPS) and the F2-layer peak electron density obtained  from Digisonde data have been used to study the diurnal, seasonal and solar activity variations of the ionospheric equivalent slab thickness  (s) over three European stations located at Pruhonice (50.0N,15.0E), Ebro (40.8N,0.5E) and El Arenosillo (37.1N,353.3E).  The diurnal variation of the s is characterized by daytime values lower than nighttime ones for all seasons at low solar activity while  daytime values larger than nighttime characterizes the diurnal variation for summer at high solar activity. A double peak is noticeable  at dusk and at dawn, better expressed for winter at low solar activity. The seasonal variations of s depend on local time and solar activity,  the daytime values of s increases from winter to summer whereas nighttime values of s show the opposite. The effect of the solar activity  on s depends on local time and season, there being very sensitive for winter nighttime values of s. The results of this study are compared  with those presented by other authors.","palabras_clave":"Ionospheric slab thickness|F2-layer peak|Total Electron Content"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63472,"idioma_id":4,"titulo":"Fermentation of quinoa and wheat slurries by Lactobacillus plantarum CRL 778: proteolytic activity","resumen":"Quinoa fermentation by lactic acid bacteria (LAB) is an interesting alternative to produce new bakery products with high nutritional value; furthermore, they are suitable for celiac patients because this pseudocereal contains no gluten. Growth and lactic acid production during slurry fermentations by Lactobacillus plantarum CRL 778 were greater in quinoa (9.8 log cfu\/mL, 23.1 g\/L) than in wheat (8.9 log cfu\/mL, 13.9 g\/L). Lactic fermentation indirectly stimulated flour protein hydrolysis by endogenous proteases of both slurries. However, quinoa protein hydrolysis was faster reaching 40-100% at 8 h of incubation while wheat protein hydrolysis was only 0-20%. In addition, a higher amount of peptides (24) and free amino acids (5g\/L) were determined in quinoa compared to wheat. Consequently, greater concentrations (ca. 2.6-folds) of the antifungal compounds (phenyllactic and hydroxyphenyllactic acids) were synthesized from Phe and Tyr in quinoa by L. plantarum CRL 778, an antifungal strain. These promising results suggest this LAB strain could be used in the formulation of quinoa sourdough to obtain baked goods with improved nutritional quality and shelf life, suitable for celiac patients.","palabras_clave":"Quinoa|Proteolysis|Lactobacillus|Fermentation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63473,"idioma_id":4,"titulo":"Metabolomics as a tool for the comprehensive understanding of fermented and functional foods with lactic acid bacteria","resumen":"        Metabolomics,  also called metabonomics or metabolic profiling, deals with the simultaneous  determination and quantitative analysis of intracellular metabolites or low-molecular-mass  molecules. The metabolomics field, which has begun a little more than ten years  ago thanks to the development of technologies such as nuclear magnetic  resonance (NMR) and mass spectrometry (MS), has been successfully applied in  different areas of food science. This review deals with the recent achievements  of metabolomics in the comprehensive analysis of fermented foods predominated by  lactic acid bacteria, the fermentative capacity of these microorganisms and the  beneficial effects of functional foods and probiotics&nbsp;   ","palabras_clave":"METABOLOMICS|METABOLOMIC PROFILING|FERMENTED FOODS|PROBIOTICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63474,"idioma_id":4,"titulo":"Diversity in proteinase specificity of thermophilic lactobacilli as revealed by hydrolysis of dairy and vegetable proteins","resumen":"Ability of industrially relevant species of thermophilic lactobacilli strains to hydrolyze proteins from animal (caseins and &#946;-lactoglobulin) and vegetable (soybean and wheat) sources, as well as influence of peptide content of growth medium on cell envelope-associated proteinase (CEP) activity, was evaluated. Lactobacillus delbrueckii subsp. lactis (CRL 581 and 654), L. delbrueckii subsp. bulgaricus (CRL 454 and 656), Lactobacillus acidophilus (CRL 636 and 1063), and Lactobacillus helveticus (CRL 1062 and 1177) were grown in a chemically defined medium supplemented or not with 1 % Casitone. All strains hydrolyzed mainly &#946;-casein, while degradation of &#945;s-caseins was strain dependent. Contrariwise, &#954;-Casein was poorly degraded by the studied lactobacilli. &#946;-Lactoglobulin was mainly hydrolyzed by CRL 656, CRL 636, and CRL 1062 strains. The L. delbrueckii subsp. lactis strains, L. delbrueckii subsp. bulgaricus CRL 656, and L. helveticus CRL 1177 degraded gliadins in high extent, while the L. acidophilus and L. helveticus strains highly hydrolyzed soy proteins. Proteinase production was inhibited by Casitone, the most affected being the L. delbrueckii subsp. lactis species. This study highlights the importance of proteolytic diversity of lactobacilli for rational strain selection when formulating hydrolyzed dairy or vegetable food products.","palabras_clave":"proteolysis|lactic acid bacteria|proteinase|inhibition"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63475,"idioma_id":4,"titulo":"RETRIEVAL OF RELEVANT PARAMETERS OF NATURAL MULTILAYER       SYSTEMS BY MEANS OF BIO-INSPIRED OPTIMIZATION STRATEGIES","resumen":"Natural photonic structures exhibit remarkable color effects such as metallic appearance and iridescence. A  rigorous study of the electromagnetic response of such complex structures requires to accurately determine  some of their relevant optical parameters, such as the refractive indices of the materials involved. In this  paper, we apply different heuristic optimization strategies to retrieve the real and imaginary parts of the  refractive index of the materials comprising natural multilayer systems. Through some examples, we  compare the performances of the inversion methods proposed and show that these kinds of algorithms have  a great potential as a tool to investigate natural photonic structures.","palabras_clave":"structural color|photonic structures|multilayers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63476,"idioma_id":4,"titulo":"In-plane Displacement measurement in Vortex Metrology by Synthetic Network Correlation Fringes","resumen":"  Recently we proposed an alternative method of displacement analysis in vortex metrology, based on the application  of the Fourier optics techniques, that is suitable for an intermediate range of displacement measurements  ranging below the resolution of speckle photography and above that of the conventional vortex metrology. However,  for smaller displacements, we introduce an approach to perform the Fourier analysis from vortex networks.  In this work, we present an enhanced method for measuring uniform in-plane displacements, taking advantage of  the capability of determining the subpixel locations of vortices and having the ability to track the homologous  vortices onto a plane. It is shown that high-quality fringe systems can be synthesized and analyzed to accurately  measure in an extended range of displacements and for highly decorrelated speckle patterns. Experimental results  supporting the validity of the method are presented and discussed","palabras_clave":"OPTICAL VORTEX METROLOGY|SPECKLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63477,"idioma_id":4,"titulo":"Ab initio potential for the He-Ag(110) interaction investigated using grazing-incidence fast-atom diffraction","resumen":"Experimental diffraction patterns produced by grazing scattering of fast helium atoms from a Ag(110) surface are used as a sensitive tool to test an ab initio potential model derived from accurate density-functional theory(DFT) calculations. The scattering process is described by means of the surface eikonal approximation, which is a distorted-wave method that includes the quantum interference between contributions coming from different projectile paths, taking into account the complete corrugation of the three-dimensional projectile-surface potential. A fairly good agreement between the theoretical and experimental momentum distributions is found for incidence along different low-indexed crystallographic directions. This agreement is indicative of the quality of the DFT potential. The effective corrugation of the interaction potential across the incidence channel is also investigated.","palabras_clave":"diffraction|crystal|atom|grazing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63478,"idioma_id":4,"titulo":"Interaction potentials for multi-electron atoms in front of a LiF (001) surface 3 from rainbow scattering","resumen":"Pairwise interaction potentials for multi-electron atoms moving in front of a LiF (001) surface are investigated theoretically and experimentally. From angular distributions of fast He, N, S, Cl and Kr atoms grazingly scattered under axial surface channeling conditions, rainbow angles are experimentally determined for a wide range of energies for the motion normal to the surface plane. These angles are used as a benchmark to probe the pairwise potential model. In the simulations the scattering process is described by means of the surface eikonal approximation, while the atom?surface interaction is derived by adding binary interatomic potentials that include the proper asymptotic limit.","palabras_clave":"rainbow|surface|insulator|potential"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63479,"idioma_id":4,"titulo":"The role of irreversible and reversible phenomena in the piezoresistive behavior of graphene epoxy nanocomposites applied to structural health monitoring","resumen":"The use of graphene for the development of a strain and damage sensor was evaluated and modeled. To achieve this, a graphene epoxy reactive mixture was used as a conductive coating which was cured onto a carbon fiber reinforced composite. This methodology proved to be very effective where substantial changes in piezoresistivity (up to 400%) were found as a function of strain (up to 2%). This contributed to a very high linear gauge factor (56.7±0.69). The role of reversible and irreversible phenomena in the sensor piezoresistivity was modeled using the concepts of tunneling currents and conduction paths. In order to predict the response at higher deformation, an irreversible component was introduced to account for the substantial increase of piezoresistivity. A model which incorporated both components was able to predict the piezoresistivity up to high deformation.","palabras_clave":"graphene|structural health monitoring"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63480,"idioma_id":4,"titulo":"SEMANTICS AND LEGAL INTERPRETATION. AN APPROACH FROM A COMPARATIVE STUDY OF THE VALUE OF EMBRYONIC LIFE UNDER THE ARGENTINE AND U.S. CONSTITUTIONAL CASE LAW","resumen":"The question regarding the legal status of the embryo hinges around a more conceptual or, rather, more fundamental legal distinction, namely, the distinction between things and persons. What is involved here is determining whether embryonic human life is personal life and, thus, whether the embryo has rights or whether is it just the object of somebody else´s rights.  The claim that the legal value of embryonic life depends upon its stage of development and its viability perspectives is one of the main arguments in favor of a right to abortion in American constitutional Case law and, extensively, in favor of the right -and sometimes duty- to discard embryos (section 2). On the other hand, Argentine constitutional case law -with some exceptions- acknowledges a personal nature in each and every human being from the time of conception, which is, in turn, placed at the moment of fertilization (setion 3).   Although the arguments raised in each of these legal practices for or against embryonic life legal protection arise in different normative contexts, the creative nature of constitutional interpretation justifies the comparative approach propounded in this review. But creativity in interpretation operates within the frame given by two margins: the teleological one and the linguistic or, more generally, the semantic one. With these restrictions in mind, the questions that this comparative study aims at answering are: (a) Which is the justificatory or teleological perspective of interpretation assumed or postulated in each of these case law practices? ; (b) Which is the semantic theory undergrounding the whole interpretative process in each of these case law practices?; (c) Which of these teleological and semantic postulates best fit the final aims or values of constitutional Law?.  In the end, we aim at reflecting upon the reciprocal influence between these two margins of interpretation. Particularly, we aim at testing the coherence between, on the one side, the claim that fundamental rights are deontological and, on the other, the assumption of a constructive or criterial semantic theory of language when interpreting the concept of legal personhood.","palabras_clave":"semántica|embrión|realismo|convencionalismo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63481,"idioma_id":5,"titulo":"El rol de las poblaciones marginales en la conservación del acervo genético de la única conífera del sur de Yungas en Argentina y Bolivia, Podocarpus parlatorei (Podocarpaceae)","resumen":"La conservación del acervo genético asegura el potencial evolutivo y los eventos de especiación.Para desarrollar potenciales planes de conservación, uso sustentable y manejo de las especies,es importante conocer los patrones de distribución de las variantes genéticas y de la diversidadgenética. Podocarpus parlatorei (Podocarpaceae) es una especie protegida bajo el Apéndice I deCITES y es la única conífera nativa tolerante al frío que habita la selva montana del extremo surde Yungas subtropicales. Presenta una distribución naturalmente fragmentada, que abarca 13°de latitud sobre la cordillera Oriental. En este trabajo analizamos 21 poblaciones de Argentinay Bolivia, que incluyen todo el rango de distribución, con dos marcadores moleculares distintospara analizar el rol de las poblaciones marginales como reservorio de variantes particulares y dediversidad genética. Utilizamos electroforesis de isoenzimas y secuencias de ADN del cloroplasto.Calculamos parámetros de diversidad genética, aplicamos análisis multivariados de agrupamientoen base a distancias genéticas y bayesiano de estructuración. Los resultados ponen de manifiestoque existen variantes genéticas únicas para ambos marcadores en las poblaciones del sur de ladistribución y para poblaciones del norte. La distribución de la diversidad genética entre ambosmarcadores difiere, siendo para isoenzimas las poblaciones del sur las más diversas y para ADNdel cloroplasto las del centro de la distribución. Las poblaciones con alta diversidad y haplotiposúnicos no se encuentran dentro de áreas protegidas, por lo que sugerimos que algunas poblacionessean consideradas en futuros planes de conservación.","palabras_clave":"Bosque montano|Filogeografía|Genética de poblaciones|Podocarpáceas|Sudamérica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63482,"idioma_id":4,"titulo":"Fine-scale genetic structure of Nothofagus pumilio (lenga) at contrasting elevations of the altitudinal gradient","resumen":"Montane forests provide the natural framework to test for various ecological settings at distinct elevations as they may affect population demography, which in turn will affect the spatial genetic structure (SGS). We analyzed the fine-scale SGS of Nothofagus pumilio, which dominates mountain areas of Patagonia, in three pairs of sites at contrasting elevations (low- vs. high-elevation). Within a total area of 1 ha fresh leaf tissue from 90 individuals was collected at each of the six studied stands following a spatially explicit sampling design. Population genetic diversity parameters were analyzed for all sampled individuals using five polymorphic isozyme loci, and a subset of 50 individuals per stand were also screened for five microsatellite loci. The SGS was assessed on 50 individuals \/ stand, using the combined datasets of isozymes and microsatellites. Most low-elevation stands consisted of older individuals with complex age structures and genetically diverse plots. In contrast, high-elevation stands and one post-fire low-elevation population yielded even-aged structures with evidence of growth suppression, and were genetically homogeneous. All stands yielded significant SGS. Similarly to mature stands of the non-sprouter congener N. dombeyi, multi-age low-altitude N. pumilio yielded significant SGS weakened by competing species of the understory and the formation of seedling banks. Alike the sprouter N. antarctica, high-altitude stands produced significant SGS as a consequence of occasional seedling establishment reinforced by vegetative spread.","palabras_clave":"CLONALITY|ISOZYMES|MICROSATELLITES|NOTHOFAGUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63483,"idioma_id":4,"titulo":"Reactive sputter magnetron reactor for preparation of thin films and simultaneous in situ structural study by X-ray diffraction","resumen":"The purpose of the designed reactor is (i) to obtain polycrystalline   and\/or amorphous thin films by controlled deposition induced by a   reactive sputtering magnetron and (ii) to perform a parallel in situ   structural study of the deposited thin films by X-ray diffraction, in   real time, during the whole growth process. The designed reactor allows   for the control and precise variation of the relevant processing   parameters, namely, magnetron target-to-sample distance, dc magnetron   voltage, and nature of the gas mixture, gas pressure and temperature of   the substrate. On the other hand, the chamber can be used in different   X-ray diffraction scanning modes, namely, &#952;-2&#952; scanning, fixed &#945;-2&#952;   scanning, and also low angle techniques such as grazing incidence small   angle X-ray scattering and X-ray reflectivity. The chamber was mounted   on a standard four-circle diffractometer located in a synchrotron beam   line and first used for a preliminary X-ray diffraction analysis of AlN   thin films during their growth on the surface of a (100) silicon wafer.","palabras_clave":"ALUMINIUM COMPOUNDS|GAS MIXTURES|III-V SEMICONDUCTORS|SEMICONDUCTOR GROWTH|SEMICONDUCTOR THIN FILM|SPUTTER DEPOSITION|SYNCHROTRONS|WIDE BAND GAP SEMICONDUCTORS|X-RAY DIFFRACTION|X-RAY DIFFRACTOMETERS|X-RAY REFLECTION|X-RAY SCATTERING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63484,"idioma_id":4,"titulo":"The Adventitia Layer Modulates the Arterial Wall Elastic Response to Intra-Aortic Counterpulsation: In Vivo Studies","resumen":"Background: there is a relationship between the benefits of Intra-Aortic Balloon Pumping (IABP) and the dynamic behavior of muscular arteries, which is associated with induced changes on the vessel walls due to an endothelial-dependent mechanism. On the other hand, the arterial wall elastic behavior is influenced by adventitial function; however, no studies were undertaken in order to elucidate if this layer plays a role in the changes determined by IABP. Aims: to quantify acute IABP effects on the elasticity of iliac arteries before and after removing the adventitia, and during acute heart failure (AHF) in de-adventialized iliac arteries. Methods: pressure and diameter signals were recorded in the iliac arteries of sheep (n=7), before and during 1:2 IABP: (1) in control state with intact iliac arteries, (2) in control state after iliac arteries adventitia removal, and (3) in de-adventitialized iliac arteries after AHF. Conduit function, compliance and arterial distensibility were calculated for each state. Results: (1) during Control State, IABP resulted in intact iliac artery dilatation and in an increase in conduit function, compliance and distensibility (p&lt;0.05); (2) adventitial removal determined a decrease of iliac artery compliance and conduit function with respect to the control state (p&lt;0.05), which increased when IABP was used (p&lt;0.05); (3) the increase in iliac artery stiffness observed after adventitia removal was also detected in AHF state; (4) IABP improves conduit function and arterial wall elasticity in de-adventitialized arteries during AHF (p&lt;0.05). Conclusion: the stiffness reduction observed in intact iliac arteries subjected to IABP was also observed after adventitia removal. Furthermore, during AHF, the augmented vessel wall stiffness was reduced by IABP in de-adventitialized iliac arteries.  ","palabras_clave":"Adventitia|Acute heart failure |Arterial stiffness  |Intra-aortic balloon pumping"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63485,"idioma_id":4,"titulo":"Stability of expanded austenite, generated by ion carburizing and ion nitriding of AISI 316L SS, under high temperature and high energy pulsed ion beams irradiation","resumen":"Expanded austenite can be generated on austenitic stainless steels either by ion carburizing or ion nitriding. In both cases the resulting fcc crystal structure, supersaturated with nitrogen or carbon, is strongly hardened with improved wear-resistance, while maintaining the original resistance to corrosion. In this work, we have studied the stability of expanded austenite, generated by ion nitriding and ion carburizing on AISI 316L SS with N and C, under: a\u2014high temperature (225&nbsp;°C \u2013 504&nbsp;°C), and b\u2014under irradiation with high energy (30&nbsp;keV \u2013 500&nbsp;keV), high fluence (~&nbsp;1015&nbsp;cm&#8722;&nbsp;2), short duration (~&nbsp;400&nbsp;ns) light (deuterium and helium) ion beams. It was found that expanded austenite is stable below 325&nbsp;°C. Between 325&nbsp;°C and 504&nbsp;°C expanded austenite lattice parameter presents gradual reduction with increasing temperature. We observed microstructural changes related only to the temperature treatment. We did not observe any microstructure change due to the duration of the heat treatment. Over 504&nbsp;°C, the lattice parameter returns to the material's austenite original parameter. On the other hand, when irradiated with pulsed ion beams, a gradual reduction of the lattice parameter corresponding to the expanded austenite with the number of pulses was observed. This behavior can be explained through the thermal shock induced on the surface by each beam, consisting in fast heating followed by fast cooling that induces the gradual exo-diffusion of N (or C). Nevertheless, after 20 ion pulses, a final lattice parameter slightly higher than the corresponding to the original austenite was found as stable limit. This residual expansion can be attributed to partial amorphization of the first few micrometers that induces stresses on the crystals of austenite which are closer to the surface layers.","palabras_clave":"ION CARBURIZING|EXPANDED AUSTENITE|PLASMA FOCUS|CRYSTALLINE STABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63486,"idioma_id":5,"titulo":"ACTIVIDAD ELECTRICA ASOCIADA A  LA ERUPCION DEL COMPLEJO VOLCÁNICO CORDÓN CAULLE  DURANTE 2011","resumen":"Se realizó un estudio de la actividad eléctrica generada por la erupción del complejo volcánico Cordón Caulle en junio de 2011 utilizando datos de descargas eléctricas detectadas por la red World Wide Lightning Location Network. La actividad eléctrica observada se corresponde a descargas eléctricas generadas en la pluma volcánica con una buena correlación entre la cantidad de descargas y la altura de la misma. Aunque la actividad eléctrica detectada fue baja, los resultados están de acuerdo con los obtenidos por estudios previos y muestran que puede ser usada como un indicador de la actividad volcánica. Además muestra la factibilidad de utilizar la red para una detección temprana de erupciones volcánicas.","palabras_clave":"actividad electrica|cordon Caulle"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63487,"idioma_id":4,"titulo":"Nitrated fatty acids: Synthesis and measurement","resumen":"Ver PDF","palabras_clave":"NITRATE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63488,"idioma_id":5,"titulo":"DESEMPEÑO DEL PRONÓSTICO DE MODELOS DE ALTA RESOLUCIÓN, EN UN ÁREA LIMITADA: ANÁLISIS DE LA ESTACIÓN DE VERANO 2010-2011","resumen":"Los modelos  numéricos constituyen hoy en día una herramienta indispensable para  la comunidad meteorológica, tanto para fines operativos como de  investigación. En  nuestra región, los modelos BRAMS y WRF han sido utilizados con  diversos fines. En particular, a partir de Noviembre de 2010 se  procesa en el Servicio Meteorológico Nacional, una versión del  BRAMS de pronóstico diario a 18 horas de alta resolución sobre la  Provincia de Buenos Aires.Dado  que el uso del modelo WRF se está incrementando año a año en la  comunidad científica, es el objetivo de este trabajo hacer una  comparación entre pronósticos generados por el WRF y los que se  procesan diariamente en el SMN con el BRAMS, utilizando anidados de  resolución mayor a la utilizada hasta el momento en nuestro país en  tiempo real. Se evalúa el desempeño de ambos durante el período de  verano del Hemisferio Sur 2010 - 2011. A  partir del análisis de la precipitación se pudo concluir que  mediante el uso de modelos de mayor resolución se pueden representar  máximos de precipitación en casos de precipitación intensa, que no  se logran detectar con el modelo de menor resolución. Además, se  realizó la verificación del perfil vertical termodinámico en la  estación aerológica de Ezeiza, la cual mostró que ambos modelos  poseen habilidad en representar la estructura vertical de la  atmósfera, aunque con algunas limitaciones en la capa límite.","palabras_clave":"PRONÓSTICO NUMÉRICO|DESEMPEÑO|ALTA RESOLUCIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63489,"idioma_id":4,"titulo":"Quantitative risk assessment of human campylobacteriosis by consumption of salad cross-contaminated with thermophilic Campylobacter spp. from broiler meat in Argentina","resumen":"Here, we developed a quantitative risk assessment for thermophilic Campylobacter spp. related to the consumption of salad prepared alongside broiler meat. The assessment considered initial contamination levels, cross-contamination and decontamination events during the broiler slaughter process and distribution, and storage and consumption patterns in Argentina and other Latin American countries. The model predicted an infection risk of 3.32x10-4 per serving. This estimation was variable according to the dose-response model used. Considering the number of chickens slaughtered annually in Argentina, the estimated number of people who could suffer campylobacteriosis related to poultry meat consumption was, on average, 484,304. The risk of human campylobacteriosis was most sensitive to the probability of infection from a Campylobacter (r= 0.72), the number of Campylobacter spp. per serving (r= 0.40), the frequency of washing the cutting board (r= -0.31), the preparation of raw poultry before salad using the same cutting board (r= 0.14), and the frequency of hand washing (r = -0.14). The most sensitive stages of the process identified through the risk assessment can be used as a basis for measures of risk management. Public campaigns on hygiene habits during food preparation at home should focus on the importance of washing the cutting board before preparing raw and ready-to-eat foods and of washing the hands during food preparation.","palabras_clave":"QUANTITATIVE RISK ASSESSMENT|CAMPYLOBACTER|BROILER MEAT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63490,"idioma_id":4,"titulo":"Identification of lactic acid bacteria with potential biopreservative isolated from slaughterhouse avian blood","resumen":"Blood is a common by-product of the meat industry, which has several potential applications in the animal feed industry. However, since blood is highly susceptible to microbial spoilage, blood and its fractions are often not suitable ingredients for the feed industry. Biopreservation appears as an alternative for the improvement of blood?s quality towards its use as an ingredient in foodstuff. The objective of this work was to isolate and identify Lactic Acid Bacteria (LAB) in avian blood obtained from industrial slaughterhouses and evaluate their antimicrobial activity. Ninety-six LAB were isolated from avian blood and genotyped. Eleven Amplified rDNA Restriction Analysis groups were identified.  Between two and five different species were detected in each blood sample (31 strains in all blood samples) and these were selected for study antagonistic activity. Twenty-eight out of these produced antimicrobial compounds such as organic acids, 11 strains produced hydrogen peroxide (H2O2), and two released bacteriocin-like compounds. The latter, identified as Lactobacillus salivarius (DSPV 027SA) and Enterococcus faecalis (DSPV 008SA), inhibited the growth of Escherichia coli, Pseudomonas aeruginosa and some serotypes of Salmonella. These two LAB strains would be candidates for potential application as a blood biopreservation system. This biotechnological tool is cheaper than others sanitation techniques and could reduce the risk of pathogens transmission thought food chain.","palabras_clave":"Lactic acid bacteria,| Avian blood, |Biopreservation|Bacteriocins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63491,"idioma_id":4,"titulo":"Mechanical and transport properties of ten years old concretes prepared with different coarse aggregates","resumen":"Many studies have discussed the effects of coarse aggregate on the mechanical properties of concrete, but this is not the case of the transport properties. The evaluation of the behaviour of old concretes becomes of interest as the presence of microcracks can modify its response. Concretes prepared with different coarse aggregates (granitic and quartzitic crushed stone and river gravel, maximum sizes 19 and 38&#65440;mm damaged by drying shrinkage after the exposure during 10 years to moderate environmental conditions are studied. To analyze greater levels of damage the residual properties of the same concretes after high temperature exposure or load-induced cracking are also studied. The paper includes the evaluation of mechanical and transport properties together with a characterization of the internal structure of concrete (density of cracks, density and perimeter of coarse aggregates). The compressive strength decreased after 10 years exposed to laboratory ambient; the concrete prepared with river gravel 38 mm maximum size showed the largest reductions while concretes prepared with quartzitic stone maintained the initial strength level. The different damage processes affect the transport properties in different manner according to the distribution and density of induced cracks; water permeability seems to be the mechanism most sensitive to the type and size of coarse aggregate.","palabras_clave":"Coarse aggregate type|Damage|Microcracking|Transport properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63492,"idioma_id":4,"titulo":"Structural basis for feed-forward transcriptional regulation of membrane lipid homeostasis in Staphylococcus aureus","resumen":"    The biosynthesis  of membrane lipids is an essential pathway for virtually all bacteria. Despite  its potential importance for the development of novel antibiotics, little is  known about the underlying signaling mechanisms that allow bacteria to control their  membrane lipid composition within narrow limits. Recent studies disclosed an  elaborate feed-forward system that senses the levels of malonyl-CoA and  modulates the transcription of genes that mediate fatty acid and phospholipid synthesis  in many Gram-positive bacteria including several human pathogens. A key  component of this network is FapR, a transcriptional regulator that binds malonyl-CoA,  but whose mode of action remains enigmatic. We report here the crystal structures  of FapR from Staphylococcus aureus (SaFapR) in three relevant states of its regulation cycle. The  repressor-DNA complex reveals that the operator binds two SaFapR homodimers with different affinities, involving sequence-specific contacts  from the helix-turn-helix motifs to the major and minor grooves of DNA. In  contrast with the elongated conformation observed for the DNA-bound FapR  homodimer, binding of malonyl-CoA stabilizes a different, more compact, quaternary  arrangement of the repressor, in which the two DNA-binding domains are attached  to either side of the central thioesterase-like domain, resulting in a  non-productive overall conformation that precludes DNA binding. The structural transition  between the DNA-bound and malonyl-CoA-bound states of SaFapR involves substantial changes and large (.30 A° )  inter-domain movements; however, both conformational states can be populated by  the ligand-free repressor species, as confirmed by the structure of SaFapR in two distinct crystal forms. Disruption of the ability of SaFapR to monitor malonyl-CoA compromises cell growth, revealing the  essentiality of membrane lipid homeostasis for S. aureus survival and uncovering novel opportunities for the development of  antibiotics against this major human pathogen.","palabras_clave":"Lipid Homeostasis|Transcriptional Regulation|Staphylococcus aureus|Antibiotic target"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63493,"idioma_id":4,"titulo":"Activation of brain areas concerned with social cognition during  moral decisions is abnormal in schizophrenia patients and unaffected siblings","resumen":"Moral decision-making involves complex social cognitive processes which   are known to be altered in patients with schizophrenia and first-degree   relatives. Traditional philosophical views on human moral behavior have   distinguished between utilitarian views (which emphasize outcomes) and   deontological approaches (defining what is right to do according to   certain norms). Since emotions have been suggested to play a determining   role in moral behavior, we hypothesized patients with schizophrenia and   unaffected siblings would make more utilitarian choices and show faulty   activation of brain areas concerned with emotion regulation during such   tasks. Unexpectedly, all participants (n&nbsp;=&nbsp;13 per group) made   the same proportion of utilitarian and deontological decisions. Brain   activation common to all groups induced by moral decisions included two   circumscribed portions of right ventromedial and dorsolateral prefrontal   cortex, adding to previous evidence on a right prosencephalic cognitive   network involved in ethical decisions. However, brain activation   induced by moral decisions was different in healthy persons,   schizophrenia patients, and nonpsychotic siblings in regards to areas   directly concerned with emotion processing. These results seem to   underscore the role of acquired norms in moral decisions, a frequently   overlooked concept in the neurobiological characterization of human   ethical behavior, and add to previous evidence of abnormal social   cognitive processing in schizophrenia.","palabras_clave":"Moral|Emotion|Schizophrenia|Siblings"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63494,"idioma_id":4,"titulo":"Kinetics and functional diversity among the five members of the NADP-malic enzyme family from Zea mays, a C 4 species","resumen":"NADP-malic enzyme (NADP-ME) is involved  in different metabolic pathways in several organisms due to  the relevant physiological functions of the substrates and  products of its reaction. In plants, it is one of the most  important proteins that were recruited to fulfil key roles in  C4 photosynthesis. Recent advances in genomics allowed  the characterization of the complete set of NADP-ME  genes from some C3 species, as Arabidopsis thaliana and  Oryza sativa; however, the characterization of the complete  NADP-ME family from a C4 species has not been performed  yet. In this study, while taking advantage of the  complete Zea mays genome sequence recently released, the  characterization of the whole NADP-ME family is presented.  The maize NADP-ME family is composed of five  genes, two encoding plastidic NADP-MEs (ZmC4- and  ZmnonC4-NADP-ME), and three cytosolic enzymes  (Zmcyt1-, Zmcyt2-, and Zmcyt3-NADP-ME). The results  presented clearly show that each maize NADP-ME displays  particular organ distribution, response to stress  stimuli, and differential biochemical properties. Phylogenetic  footprinting studies performed with the NADP-MEs  from several grasses, indicate that four members of the  maize NADP-ME family share conserved transcription  factor binding motifs with their orthologs, indicating conserved  physiological functions for these genes in monocots.  Based on the results obtained in this study, and considering  the biochemical plasticity shown by the NADP-ME, it is  discussed the relevance of the presence of a multigene  family, in which each member encodes an isoform with  particular biochemical properties, in the evolution of the C4  NADP-ME, improved to fulfil the requirements for an  efficient C4 mechanism.","palabras_clave":"MAIZE|NADP-MALIC ENZYME|C4 PHOTOSYNTHESIS|ORTHOLOGS|EVOLUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63495,"idioma_id":4,"titulo":"Gas-phase conversion of 1,3-butanediol on single acid-base and Cu-promoted oxides","resumen":"The gas-phase conversion of 1,3-diols was studied on catalysts containing different active sites and using1,3-butanediol as a model molecule. Transformation of the diol primary and\/or secondary OH groups bydehydration and dehydrogenation reactions yields valuable oxygenates combining OH and C C, OH andC O or C C and C O functions, along with other compounds. A series of single oxides with differentacid?base properties, copper?silica and copper-single oxide catalysts were prepared by several methodsand characterized by TPR, XRD and N2O chemisorption.Acid?base oxides transform 1,3-butanediol at low rates. Oxide electronegativity determines the mainreaction pathway: acidic oxides promote 1,3-butanediol dehydration toward unsaturated alcohols andolefins, whereas basic oxides dehydrogenate-dehydrate the diol toward the unsaturated ketone.The effect of Cu0in monofunctional copper?silica, or bifunctional copper\/acid or copper\/base catalysts,is to increase the reaction rate and to shift the reaction pathway toward 1,3-butanediol dehydro-genation. The metal dispersion depends on the preparation method by impregnation, ion exchange orco-precipitation. Small Cu0particles strongly adsorb reactant and products allowing consecutive reac-tions to take place after initial dehydrogenation. In bifunctional catalysts, the role of the acid or base sitesis to promote consecutive dehydration-hydrogenation or C C bond cleavage reactions, respectively.","palabras_clave":"DEHYDRATION|DEHYDROGENATION|DIOLS|ACID-BASE CATALYSIS|COPPER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63496,"idioma_id":4,"titulo":"Conversion of diols by dehydrogenation and dehydration reactions on silica-supported copper catalysts","resumen":"The gas-phase conversion of a 1,3-polyol (1,3-butanediol) containing primary and secondary OH functionswas studied on a series of copper-silica catalysts, ZCuSiO2 (Z = 1¨C25 wt.% Cu), and thoroughly characterized by several techniques such as BET surface area, TPR, XRD, N2O chemisorption, and UV-vis-DRS. The physicochemical properties of the ZCuSiO2 catalysts depended on whether the metal loading was above or below the copper monolayer surface coverage (Z = 13.5 wt.% Cu). Copper species presenting differentdegrees of interaction with the silica support were detected. At low Z values Cu0 dispersion was high (D¡Ö40%) due to a predominant contribution of nano-sized Cu species (3 nm) which are difficult to reduce, but for Z &gt; 13.5 wt.%, D abruptly dropped to 3¨C8% because of formation of larger tridimensional Cu clustered species (30 nm) that reduced at lower temperatures because of a decreased copper-silicainteraction. On ZCuSiO2 catalysts, dehydrogenation of the 1,3-butanediol secondary OH function prevailed over that of the primary one and therefore valuable ketones were the main reaction products. Consecutivelyto dehydrogenation, dehydration and hydrogenation reactions also took place. Products of the tandemreaction were the -hydroxy ketone (4-hydroxy-2-butanone), the ,-unsaturated ketone (methyl vinyl ketone) and the saturated ketone (methyl ethyl ketone). A direct 1,3-butanediol dehydration pathwaytoward methyl ethyl ketone was also found. Reaction pathways were strongly dependent on the Cu loadingand therefore on the kind of Cu species (isolated or clustered). When compared at similar conversionlevels, selectivity to the dehydrogenation product 4-hydroxy-2-butanone increased with Z suggestingthat on large Cu0 particles 4-hydroxy-2-butanone was released to the gas phase before being converted in consecutive steps. On the contrary, on highly dispersed Cu0 crystals of low Cu loading catalysts, 1,3-butanediol was readily dehydrated giving the saturated ketone.Kinetically relevant reaction steps of 1,3-butanediol conversion by dehydrogenation and dehydrationwere promoted on Cu0 sites. Dehydration of the intermediate 4-hydroxy-2-butanone also occurred on Cu0 sites. Turnover rates were constant on Cu0 nano particles and slightly higher on clustered species.","palabras_clave":"1,3-BUTANEDIOL|DEHYDRATION|DEHYDROGENATION|COPPER|BIOMASS UPGRADING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63497,"idioma_id":4,"titulo":"Measurements of natural deposition ice nuclei in Cordoba, Argentina","resumen":"Ice nucleation in the atmosphere is of practical and fundamental importance since ice crystals influence the release of snow, rain and hail. Suspended aerosols in the atmosphere can initiate freezing at temperatures below -15ºC. In this work we describe an experimental device designed to measure the concentration of natural ice nuclei under controlled temperature and supersaturation conditions. The measurements were performed at Córdoba City, for temperatures between -15ºC and -30ºC and the sampled air was supersaturated with respect to ice and subsaturated with respect to liquid water; under these conditions the deposition ice nuclei were quantified. There are few studies reported in the literature regarding measurements of deposition ice nuclei concentration and, to our knowledge, there are no previous laboratory data of this kind of ice nuclei for T &lt; -20ºC. The results show that the number of deposition ice nuclei increases at colder temperatures and higher supersaturations. These results are in general in good agreement with results previously reported by other authors. A fitting function which depends on temperature and supersaturation is proposed to parameterize the results obtained in the present work.","palabras_clave":"HETEROGENEOUS NUCLEATION|ICE NUCLEI|DEPOSITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63498,"idioma_id":5,"titulo":"El registro sedimentario Pleistoceno tardío-Holoceno de la Salina de Ambargasta (Argentina central): una aproximación paleolimnológica","resumen":"La Salina de Ambargasta es un sistema playa localizado a latitudes medias de Argentina (29°S; 64°W). El estudio de dos testigos sedimentarios, AB-1 (borde este de la salina) y AB-2 (área central), efectuado mediante un enfoque de multi-indicadores ambientales, la aplicación de un modelo sedimentario análogo y edades radiocarbónicas, permitió reconstruir la historia paleohidrológica de la salina de Ambargasta a partir del Pleistoceno tardío. La reconstrucción paleoambiental de los últimos ca. 45,000 años hasta el presente, indica seis estadios ambientales principales: 1) entre 44,700 y 39,600 años cal. AP, un intervalo seco está representado por el desarrollo de una planicie fangosa capilar; 2) entre 39,600 y 26,700 años cal. AP, un período más húmedo es registrado por facies lacustres con precipitación carbonática y sulfatada; 3) entre 26,700y 23,600 años cal. AP ocurre la fase más húmeda en Ambargasta, caracterizada por la sedimentación en una laguna efímera sulfatada, relativamente profunda, rica en tapices microbianos y marginada por planicies fangosas; 4) entre 23,600y 18,500 años cal. AP se presenta un cambio a condiciones más secas con pulsos húmedos intercalados, evidenciado por el desarrollo de planicies fangosas salinas que alternan con lagunas efímeras, y planicies fangosas capilares en las zonas supralitorales; 5) entre 18,500y 8,600 años cal. AP se identifica un intervalo más seco caracterizado por el dominio de planicies fangosas salinas y capilares, y 6) desde los 8,600 años cal. AP al presente se registra el balance hídrico más negativo en el sistema salino representado por una expansión de las planicies fangosas capilares. El control del balance hidrológico en la Salina de Ambargasta está vinculado con la variación del Sistema de tipo Monzónico Sudamericano, por lo cual la reconstrucción efectuada desde el Pleistoceno tardío permite aportar nuevas claves para comprender las fluctuaciones pasadas de este importante forzante del sistema climático de Sudamérica.","palabras_clave":"PLAYA|PALEOLIMNOLOGIA|REGISTRO SEDIMENTARIO|RECONSTRUCCION PALEOAMBIENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63499,"idioma_id":5,"titulo":"Implementation of a noise reduction algorithm in a hearing aid device based on a dsPIC","resumen":"Nowadays  there are many people affected by hearing impairments. The cost of  assistive listening devices leaves out of reach for many people a  technological solution. In the search of an economical alternative,  low-cost DSPs arises as a good choice. However, it could be difficult  to decide which has the best cost - effectiveness trade off among all  devices offered in the market. The main goal of this work, then, is  to determine the memory requirements of three noise reduction  methods. It is done in order to select the algorithm that uses fewer  resources, which will be implemented on a hearing aid device based on  a low-cost DSP. This assistive listening device through digital  signal processing techniques can get the basic features of a high-end  assistive listening device. Objective experiments are performed to  analyze the performance of the implemented system","palabras_clave":"HEARING AID DEVICE|SIGNAL PROCESSING|SNR IMPROVEMENT|CROSSCORRELATION DOA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63500,"idioma_id":5,"titulo":"Desarrollo de un sistema para adquisición y procesamiento de señales de voz para fonoaudiología","resumen":"Hoy en día muchos especialistas utilizan para el análisis de la voz medidas objetivas de la misma. Estas medidas, que generalmente son calculadas mediante programas de computadora, suelen ser complementarias a las medidas subjetivas de la voz y se utilizan para analizar la evolución del paciente a lo largo del tiempo. La principal limitación que aparece a la hora de calcular dichos parámetros es que las señales de voz son grabadas en el consultorio del especialista, donde rara vez se dispone de una cámara anecoica e insonorizada. Por ello, pueden aparecer señales espúreas que provocan mediciones erróneas. Por lo tanto, se desea desarrollar un sistema capaz de reducir el impacto de dichas señales en el resultado del análisis. Si bien hay diversas formas de eliminar componentes indeseadas de una señal, debe tenerse la precaución de no afectar las señales de interés de forma tal de que del resultado del análisis posterior no se obtengan resultados erróneos.   Por ello, se propone desarrollar un sistema capaz de adquirir sonidos mediante un arreglo de micrófonos. El equipo funciona en dos configuraciones básicas: promediación y substracción. Se realizan simulaciones para analizar el rendimiento de ambos sistemas.","palabras_clave":"ADQUISICIÓN SE SEÑALES|VOZ|PROCESAMIENTO DIGITAL DE SEÑALES|REDUCCIÓN DE RUIDO|FONOAUDIOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63501,"idioma_id":5,"titulo":"Sobre la presencia de Amaranthus palmeri (Amaranthaceae) en Argentina","resumen":"Resumen: Amaranthus palmeri, especie nativa de Estados Unidos de Norte América y México, se cita por primera vez para la fora adventicia argentina sobre la base de ejemplares coleccionados principalmente en la provincia de Córdoba, además de San Luis y La Pampa. Se describe e ilustra la especie y se menciona su distribución geográfca.     Palabras clave: Amaranthus palmeri, Argentina central, Fora adventicia.","palabras_clave":"AMARANTHUS|MALEZAS|IDENTIFICACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63502,"idioma_id":4,"titulo":"A comprehensive system for facial animation of generic 3D head models driven by speech","resumen":"A comprehensive system for facial animation of generic 3D head models riven by speech is presented in this article. In the training stage, audio-visual information is extracted from audio-visual training data, and then used to compute the parameters of a single joint audio-visual hidden Markov model (AV-HMM). In contrast to most of the methods in the literature, the proposed approach does not require segmentation\/classification processing stages of the audio-visual data, avoiding the error propagation related to these procedures. The trained AV-HMM provides a compact representation of the audio-visual data, without the need of phoneme (word) segmentation, which makes it adaptable to different languages. Visual features are estimated from the speech signal based on the inversion of the AV-HMM. The estimated visual speech features are used to animate a simple face model. The animation of a more complex head model is then obtained by automatically mapping the deformation of the simple model to it, using a small number of control points for the interpolation. The proposed algorithm allows the animation of 3D head models of arbitrary complexity through a simple setup procedure. The resulting animation is evaluated in terms of intelligibility of visual speech through perceptual tests, showing a promising performance. The computational complexity of the proposed system is analyzed, showing the feasibility of its real-time implementation.","palabras_clave":"FACIAL ANIMATION|HIDDEN MARKOV MODELS|AUDIO-VISUAL SPEECH PROCESSING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63503,"idioma_id":4,"titulo":"Real-time physical engine for floating objects with two-way fluid-structure coupling","resumen":"A method to simulate graphic animations of objects floating in a water surface in real time is presented. The fluid is simulated by means of the Lattice Boltzmann method for the shallow-waters equations and the movement of the floating objects is calculated with a Newtonian physical engine suitable for the mechanics of rigid bodies. A two-way interaction between the fluid surface and the object structures is achieved by providing inputs to the Newtonian engine representing buoyancy, drag and lift forces calculated from the solution of the Lattice Boltzmann scheme, which in turn is perturbed by displacement forces acting at the objects boundaries. The method is tested in animation scenes of boats and different adrift objects,  showing excellent rendering rates in desktop computers.","palabras_clave":"REAL-TIME ANIMATION|PHYSICS-BASED MODELLING|SHALLOW WATERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63504,"idioma_id":4,"titulo":"Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine","resumen":"The oxidation and nitration of unsaturated fatty acids transforms cell membrane and lipoprotein constituents into mediators that regulate signal transduction. The formation of 9-NO2-octadeca-9,11-dienoic acid and 12-NO2-octadeca-9,11-dienoic acid stems from peroxynitrite- and myeloperoxidase-derived nitrogen dioxide reactions as well as secondary to nitrite disproportionation under the acidic conditions of digestion. Broad anti-inflammatory and tissue-protective responses are mediated by nitro-fatty acids. It is now shown that electrophilic fatty acid nitroalkenes are present in the urine of healthy human volunteers (9.9 + 4.0 pmol\/mg creatinine); along with electrophilic 16- and 14-carbon nitroalkenyl &#946;-oxidation metabolites. High resolution mass determinations and co-elution with isotopically-labeled metabolites support renal excretion of cysteine-nitroalkene conjugates. These products of Michael addition are in equilibrium with the free nitroalkene pool in urine and are displaced by thiol reaction with HgCl2. This reaction increases the level of free nitroalkene fraction >10-fold and displays a KD of 7.5x10-6 M. In aggregate, the data indicates that formation of Michael adducts by electrophilic fatty acids is favored under biological conditions and that reversal of these addition reactions is critical for detecting both parent nitroalkenes and their metabolites. The measurement of this class of mediators can constitute a sensitive non-invasive index of metabolic and inflammatory status.","palabras_clave":"NITRO-FATTY ACID|NITRATION|ELECTROPHILE|MICHAEL ADDITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63505,"idioma_id":4,"titulo":"Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency","resumen":"            Iron is an essential  micronutrient required for a wide variety of cellular functions in plant growth  and development. Chlorosis is the first visible symptom in iron deficient  plants. Glutathione (GSH) and ascorbic acid (Asc) are multifunctional  metabolites playing important roles in redox balancing. In this work, we show  that GSH and Asc treatment prevent the chlorosis and the increase of reactive  oxygen species (ROS) concentration induced by iron deficiency in Arabidopsis  leaves. In iron deficient Arabidopsis, GSH and Asc are also able to preserve  the levels of the iron-sulfur protein ferredoxin 2 (Fd2) and the activity of  the heme protein ascorbate peroxidase (APX) at the same level found in iron  sufficient plants. GSH content decreases 25 % in iron deficient Arabidopsis seedlings,  whereas the Asc levels are not affected. All together, these results show that  GSH and Asc supplementation protect Arabidopsis seedlings from iron deficiency preserving  cell redox homeostasis and improving internal iron availability.   ","palabras_clave":"Ácido ascórbico|Glutatión|Hierro|Plantas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63506,"idioma_id":5,"titulo":"Perfil nutricional y dializabilidad de minerales de alimentos de interés social","resumen":"RESUMENIntroducción: evaluar la composicion, digestibilidad proteica (DP) y bioaccesibilidad de Fe, Zn y Ca (antes y luego de la coccion) de Alimentos de Interés Social (AIS).Material y Métodos: se analizaron 4 AIS. La composicion se determino segun AOAC (2000). La bioaccesibilidad de minerales se estimo a traves del porcentaje del mineral dializado, luego de un proceso de digestion que simula los procesos gastrointestinales. El aporte potencial (AP) de minerales se establecio como el producto de su concentracion y dializabilidad. La DP se determino por digestion enzimatica y midiendo el incremento del nitrogeno no proteico.se analizaron 4 AIS. La composicion se determino segun AOAC (2000). La bioaccesibilidad de minerales se estimo a traves del porcentaje del mineral dializado, luego de un proceso de digestion que simula los procesos gastrointestinales. El aporte potencial (AP) de minerales se establecio como el producto de su concentracion y dializabilidad. La DP se determino por digestion enzimatica y midiendo el incremento del nitrogeno no proteico.Resultados: la composicion de AIS fue la siguiente: proteinas: 11,53-24,67g\/100g; grasa: 4,31\u20138,46g\/100g; cenizas: 2,95\u20133,66g\/100g; fibra dietaria: 0,6\u20134g\/100g; carbohidratos: 49,38\u201360,37g\/100g; energia: 366,05\u2013389,38Kcal\/100g; Fe: 28,91-60,41mg\/Kg; Zn: 5,99\u201333,08mg\/Kg; Ca: 1127,69-417,39mg\/Kg; Na: 2517,21\u201313217,50mg\/Kg. La DP estuvo en un rango de 58\u201392%. Los alimentos cocidos presentaron una bioaccesibilidad de Fe y Zn menor que los alimentos crudos, lo cual puede atribuirse, en el caso del Fe, a la perdida de acido ascorbico que se produce durante la coccion, y para el Zn a interacciones con otros componentes de la matriz alimentaria que dificultan su liberacion durante los procesos digestivos. Segun su AP, los AIS cubririan entre un 10\u201326% los requerimientos de Fe, 6-8% de los requerimientos de Ca y 2\u201334% de los requerimientos de Zn.la composicion de AIS fue la siguiente: proteinas: 11,53-24,67g\/100g; grasa: 4,31\u20138,46g\/100g; cenizas: 2,95\u20133,66g\/100g; fibra dietaria: 0,6\u20134g\/100g; carbohidratos: 49,38\u201360,37g\/100g; energia: 366,05\u2013389,38Kcal\/100g; Fe: 28,91-60,41mg\/Kg; Zn: 5,99\u201333,08mg\/Kg; Ca: 1127,69-417,39mg\/Kg; Na: 2517,21\u201313217,50mg\/Kg. La DP estuvo en un rango de 58\u201392%. Los alimentos cocidos presentaron una bioaccesibilidad de Fe y Zn menor que los alimentos crudos, lo cual puede atribuirse, en el caso del Fe, a la perdida de acido ascorbico que se produce durante la coccion, y para el Zn a interacciones con otros componentes de la matriz alimentaria que dificultan su liberacion durante los procesos digestivos. Segun su AP, los AIS cubririan entre un 10\u201326% los requerimientos de Fe, 6-8% de los requerimientos de Ca y 2\u201334% de los requerimientos de Zn.Conclusiones: los AIS, poseen un buen balance nutricional. La disponibilidad de minerales de los AIS fue muy buena y se redujo ligeramente al ser cocidos.los AIS, poseen un buen balance nutricional. La disponibilidad de minerales de los AIS fue muy buena y se redujo ligeramente al ser cocidos.","palabras_clave":"Alimentos de Interés|Bioaccesibilidad de|Digestibilidad|Perfil nutricional."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63507,"idioma_id":4,"titulo":"Prevention of hydrogen peroxide-induced red blood cells lysis by Ilex paraguariensis aqueous extract: participation of phenolic and xanthine compounds","resumen":"The fresh leaves and stems of Ilex paraguariensis (Aquifoliaceae) are employed to prepare the commercial product used in North-eastern Argentina, Southern Brazil and Eastern Paraguay named mate. The presence of polyphenols and xanthines, which present antioxidant activity, is well described in I. paraguariensis. ROS play an important role in tissue damage and pathological events in living organisms affecting erythrocyte\u2019s membranes inducing to hemolysis.  Taking into account, the aim of this work was to evaluate the protective effect of an aqueous extract of I. paraguariensis (green leaves) on the hemolysis of red blood cells (RBC) induced by H2O2 and correlate this activity with the enzymatic like activity related to H2O2 metabolism. The antioxidant activity of chlorogenic acid and caffeine was also analyzed in order to investigate their participation in the activity of the crude extract. The extract as well as the compounds protected RBC from hemolysis in relation to enzymatic CAT like activity. This study could contribute to the knowledge of the antioxidant activity of I. paraguariensis in view of the great quantities of yerba mate consumed by population.","palabras_clave":"HYDRGEN PEROXIDE|ILEX PARAGUARIENSIS|HEMOLYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63508,"idioma_id":4,"titulo":"Anti-inflammatory activity of Bromelia hieronymi: comparison with bromelain","resumen":"Some plant proteases (e. g., papain, bromelain, ficin) have been used as anti-inflammatory agents for some years, and especially bromelain is still being used as alternative and\/or complementary therapy to glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. Bromelain is an extract rich in cysteine endopeptidases obtained from Ananas comosus. In this study the anti-inflammatory action of a partially purified extract of Bromelia hieronymi fruits, whose main components are cysteine endopeptidases, is presented. Different doses of a partially purified extract of B. hieronymi were assayed on carrageenan-induced and serotonine-induced rat paw edema, as well as in cotton pellet granuloma model. Doses with equal proteolytic activity of the partially purified extract and bromelain showed significantly similar anti-inflammatory responses. Treatment of the partially purified extract and bromelain with E-64 provoked loss of anti-inflammatory activity on carrageenan-induced paw edema, a fact which is consistent with the hypothesis that the proteolytic activity would be responsible for the anti-inflammatory action.","palabras_clave":"Bromelia hieronymi|Bromeliaceae|bromelain|anti-inflammatory"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63509,"idioma_id":4,"titulo":"Local and Regional Water Flow Quantification in Groundwater-dependent Wetlands","resumen":"Runoff, groundwater recharge and discharge, and surface water?groundwater  interaction are the main driving forces of wetlands. The quantification of such flows is crucial information in the restoration and management of groundwater-dependent wetlands. The objective of this work is to quantify groundwater inflow at the level of the hydrogeological basin, which discharges into the wetland in the coastal plain of the Río de la Plata  (Argentina). It also aims at evaluating whether such inflow is affected by groundwater exploitation in the high plain adjacent to the wetland. On the basis of water level data, a model of hydrological behaviour was developed and then a numerical simulation to quantify groundwater inflow was carried out. The evolution of groundwater levels was analyzed considering three situations: one of them in natural conditions and two others under different groundwater exploitation conditions. In the first case, the inflow originates in the recharge from precipitation, in the local groundwater discharge from the adjacent high plain and in the regional one from the semi-confined aquifer. The exploitation of the semi-confined aquifer in the high plain causes the formation of a cone of depression which modifies the hydrodynamics of the wetland in the area adjacent to the extraction wells. The quantification of flows shows that groundwater exploitation in areas of the basin located out of the wetland may cause the volume of water flowing into the wetland through groundwater discharge to  decrease by approximately 25 %. The importance of considering discharge wetlands as part of regional hydrogeological systems should be highlighted, mainly as regards the management of natural resources.","palabras_clave":"WETLAND |GROUNDWATER FLOW |WATER BALANCE|NUMERICAL SIMULATIONS|HYDROGEOLOGICAL SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63510,"idioma_id":4,"titulo":"Hydro-morphologic setting of the Samborombon Bay (Argentina) at the end of the 21st century","resumen":"We report on the hydrologic and morphologic setting of the Samborombon Bay, Argentina, which is expected at the end of the 21st century as a consequence of possible scenarios of relative sea level rise (RSLR). The  geomorphological analysis of the Samborombon coastland points out only minor changes occurred over the last 40 yr. The modifications are mainly related to the construction of canals to enhance the floodplain drainage. A digital elevation model (DEM) obtained by the Shuttle Radar Topography  Mission (SRTM) data archive, ad hoc calibrated\/validated for the study area, highlights that about 3000 km2 of coastal plain present a morphological setting at high risk of sea flooding. The analysis of sea level and storm surge events recorded from 1905 to 2010 in Buenos Aires provides the RSLR rate and the return period of extreme floods. In addition, vertical land movements (VLM) measured by the permanent  GPS stations of Buenos Aires and La Plata allow for the quantification of the eustatic component of the RSLR and estimating a plausible RSLR rate in the Samborombon Bay. Taking into account possible RSLR scenarios at the end of 2100 as resulting from the statistical analysis of (i) tide gauge  and GPS time series and (ii) Intergovernmental Panel on Climate  Change (IPCC) predictions, the potential effect of the increased sea level on the Samboromb´on coastland is simulated. The results show that the combined rise of sea levels, surficial waters and groundwater will lead to a new morphohydrologic setting of the coastal area, especially in the lowlying southern sector. Here, a coastline retreat up to 40 km is expected, with temporary submersion up to 4000 km2 during storm surges.","palabras_clave":"SIN PALABRAS CLAVES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63511,"idioma_id":4,"titulo":"Recharge assessment in an urban area: a case study of La Plata, Argentina","resumen":"Leakage from water mains, storm drainage and sewer systems in urban areas constitutes a source of recharge that is difficult to identify and quantify at a regional scale. The objective of this work is to apply a methodology that would make it possible to evaluate urban recharge at a regional scale, taking as case study the city of La Plata (Argentina). In the study area, population growth and an increase in water demand caused the intensive exploitation of groundwater with the resulting alteration in groundwater flow. The methodology used was developed on the basis of a water balance and the simulation of the temporal evolution of the cones of depression and the volumes of water extracted from the aquifer. The method consists in adjusting the piezometry resulting from the numerical modelling to the measured piezometry, by means of the variation of the recharge parameter in the urban area. The results obtained make it possible to identify and quantify urban recharge, which in this case represents a volume of water similar to the recharge from precipitation.","palabras_clave":"HYDROGEOLOGY|NUMERICAL SIMULATION |HYDROGEOLOGICAL SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63512,"idioma_id":4,"titulo":"Surface water and groundwater response to the tide in coastal wetlands: Assessment of a marsh in the outer Río de la Plata estuary, Argentina","resumen":"On the coast of the outer Río de la Plata estuary (Argentina) there is a vast coastal wetland occurring in a marsh environment. The hydrodynamics of this wetland is subject to a semidiurnal microtidal regime which causes a wedge of estuary salt water to penetrate below the freshwater coming from continental discharge. This paper analyzes the hydrodynamics of surface and groundwater, and it assesses how anthropogenic changes affect the natural hydrological behavior of the coastal wetland in the southern sector of the outer Río de la Plata estuary. Water level and temperature measurements were carried out on water from the canals flowing into the estuary and the phreatic aquifer located in the marsh. The salinity of the water column was measured at one high tide which was above and one which was below the regional groundwater discharge level. The results show that in natural conditions the wetland has a complex hydrological behavior conditioned by the tidal flow. Surface and groundwater saline stratification is a distinctive characteristic, and the variations in level, temperature and salinity of groundwater depend on its interaction with estuary and continental water. At present, 47 % of the marsh is excluded from the tidal cycle due to anthropogenic action (levees, roads and canals with floodgates), causing a major alteration to the hydrological behavior and the environmental characteristics of the wetland.","palabras_clave":"INTERTIDAL WETLANDS|SURFACE WATER - GROUNDWATER INTERACTION|WATER SALINITY|ANTHPROGENIC CHANGES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63513,"idioma_id":4,"titulo":"Nitrite Reduction Mediated by Heme Models. Routes to NO and HNO?","resumen":"The  water-soluble ferriheme model FeIII(TPPS) mediates oxygen atom transfer from  inorganic nitrite to a water-soluble phosphine (tppts), dimethyl sulfide, and  the biological thiols cysteine (CysSH) and glutathione (GSH). The products with  the latter reductant are the respective sulfenic acids CysS(O)H and GS(O)H,  although these reactive intermediates are rapidly trapped by reaction with  excess thiol. The nitrosyl complex FeII(TPPS)(NO) is the dominant iron species  while excess substrate is present. However, in slightly acidic media (pH &#8776; 6),  the system does not terminate at this very stable ferrous nitrosyl. Instead, it  displays a matrix of redox transformations linking spontaneous regeneration of  FeIII(TPPS) to the formation of both N2O and NO. Electrochemical sensor and  trapping experiments demonstrate that HNO (nitroxyl) is formed, at least when  tppts is the reductant. HNO is the likely predecessor of the N2O. A key pathway  to NO formation is nitrite reduction by FeII(TPPS), and the kinetics of this  iron-mediated transformation are described. Given that inorganic nitrite has protective  roles during ischemia\/reperfusion (I\/R) injury to organs, attributed in part to  NO formation, and that HNO may also reduce net damage from I\/R, the present  studies are relevant to potential mechanisms of such nitrite protection.","palabras_clave":"Nitrite|Heme|Nitroxyl|Iron"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63514,"idioma_id":4,"titulo":"A Sweep-Heuristic Based Formulation for the Vehicle Routing Problem with Cross Docking","resumen":"Cross-docking is a warehousing strategy in logistics used by process industries making products with high proportions of distribution costs. It is described as the process of moving goods from suppliers to customers through a cross-dock terminal without a long-term storage in this facility. The vehicle routing problem with cross-docking (VRPCD) consists of fulfilling a set of transportation requests using a fleet of homogeneous vehicles to sequentially accomplish the pickup and delivery tasks. Between those operations, there is a consolidation process of incoming shipments at the cross-dock. This work introduces a monolithic formulation for the VRPCD that determines pickup\/delivery routes and schedules simultaneously with the truck scheduling at the terminal. To derive a more efficient formulation, a constraint set mimicking the widely known sweep algorithm was incorporated into the rigorous model.The resulting model based on the sweep heuristic can find near-optimal solutions to large problems at very acceptable CPU times.","palabras_clave":"CROSS-DOCKING|SUPPLY CHAIN MANAGEMENT|VEHICLE ROUTING PROBLEM|SCHEDULING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63515,"idioma_id":4,"titulo":"Enhancing fish diet analysis: equations to reconstruct Themisto gaudichaudii and Euphausia lucens length from partially digested remains","resumen":"Themisto gaudichaudii and Euphausia lucens are important prey of several fish and squid species of commercial value in the southwest Atlantic Ocean. Although allometric relationships linking size and weight can be used to estimate prey biomass, size is often difficult to obtain as they are usually partly digested. The objective of our work was to provide length-length relationships to estimate body size of both crustaceans from their remains in fish stomachs, as a first step to estimate their  weight. Measurements were performed on intact specimens collected in the field. Each of the body dimensions analysed (eye height and width for T. gaudichaudii, and carapace length and eye diameter for E. lucens) was significantly related to total length in both species. Thus, they appear to be highly reliable predictors of original size and can potentially aid in the identification of crustacean remains, allowing a more accurate analysis of digested contents.","palabras_clave":"PREY SIZE RECONSTRUCTION|ZOOPLANKTONIC CRUSTACEANS|FISH STOMACH CONTENTS|SOUTHWEST ATLANTIC OCEAN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63516,"idioma_id":5,"titulo":"Políticas públicas para la mejora de la calidad de las carreras de ingeniería: Estudio de caso del PROMEI en la Argentina","resumen":"Este trabajo analiza la asignación de fondos públicos trianuales a las universidades nacionales argentinas para que las carreras de grado reguladas por el Estado puedan implementar los planes de mejora comprometidos como resultado de la acreditación. Para ello se emplea el método de estudios de caso, seleccionando el proyecto de mayor relevancia por el monto asignado y la cantidad de carreras comprometidas: el Proyecto de Mejoramiento de la Enseñanza de las Ingenierías (PROMEI). Se analizaron documentos e indicadores cuantitativos y se realizaron entrevistas en profundidad a informantes claves y semiestructuradas a autoridades y docentes de las unidades académicas. Teniendo presente el resultado de la segunda fase de acreditación de las carreras de ingeniería, los indicadores recopilados y las percepciones de los actores entrevistados, el impacto del PROMEI sobre la mejora de la calidad de los insumos de las unidades académicas y las carreras ha sido positivo. La combinación de procedimientos de acreditación y financiamiento para la mejora muestra ser una estrategia adecuada para alinear los objetivos de las universidades (agentes) con aquellos del gobierno (principal), en lo que respecta a la formación de profesionales. No obstante, estas políticas públicas parten de un supuesto no corroborado respecto de que ciertos insumos y procesos conducen necesariamente a la formación de graduados de calidad. Es importante a futuro avanzar en la producción de información sobre distintas alternativas pedagógicas y organizacionales que contribuyan con la formación de profesionales de alto nivel académico en los distintos campos del conocimiento.","palabras_clave":"EDUCACION SUPERIOR|FINANCIAMIENTO UNIVERSITARIO |ENSEÑANZA INGENIERIA|ACREDITACION DE LA CALIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63517,"idioma_id":4,"titulo":"Chromosome evolution in Cophomantini (Amphibia, Anura, Hylinae)","resumen":"Dendropsophini is the most species rich tribe within Hylidae with 234 described species. Although cytogenetic information is sparse, chromosome numbers and morphology have been considered as an important character system for systematic inferences in this group. Using a diversity of standard and molecular techniques, we describe the previously unknown karyotypes of the genera Xenohyla, Scarthyla, and Sphaenorhrynchus, and provide new information on Dendropsophus and Lysapsus. Our results reveal significant karyotype diversity among Dendropsophini, with diploid numbers ranging from 2n = 22 in Scarthyla goinorum, 2n = 24 in Lysapsus, Scinax, Xenohyla, and almost all species of Sphaenorhynchus and Pseudis, 2n = 26 in Sphaenorhynchus carneus, 2n = 28 in Pseudis cardosoi, to 2n = 30 in all known Dendropsophus species. Although Nucleolar Organizer Regions (NORs) and C-Banding patterns show a high degree of variability, NORs positions in 2n = 22, 24, and 28 karyotypes, and C-banding patterns in Lysapsus and Pseudis, are informative cytological markers. Interstitial telomeric sequences reveal a diploid number reduction from 24 to 22 by a chromosome fusion event. The diploid number of Xenohyla truncata corroborates the character state of 2n = 30 as a synapomorphy of Dendropsophus.","palabras_clave":"B CHROMOSOMES|DENDROPSOPHINI|HYLIDAE|INTERSTITIAL TELOMERIC SEQUENCE|KARYOTYPE DIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63518,"idioma_id":5,"titulo":"La represa de Yacyretá en el desarrollo energético y territorial","resumen":"Varias décadas han pasado entre los inicios y la terminación de la represa de Yacyretá. Pero ahora genera aportes en electricidad para una Argentina con necesidades energéticas crecientes y tiende a modificar la realidad y el potencial de la región del Noreste, por largo tiempo relegada. Una mirada geohistórica dada a Yacyretá ilustra en el tiempo y en el espacio, la magnitud de esta obra pública como fuente de energía para los países y la región y como proyecto de infraestructura en un territorio. Este trabajo se apoya sobre una base empírica y en el estudio de bibliografía sobre la represa, tomando en consideración reflexiones teóricas vinculadas al tema de las redes, la coordinación entre actores y el desarrollo territorial.","palabras_clave":"hidroelectricidad|territorio|redes|integración"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63519,"idioma_id":5,"titulo":"La universidad como organización: tres enfoques para el análisis de sus problemas de gestión","resumen":"El artículo integra las contribuciones más relevantes del campo de la teoría de las organizaciones sociales para el estudio de las universidades, dividiendo los antecedentes en tres perspectivas: estructural, cultural, y política. Dentro de la perspectiva estructural se incorporan los trabajos que se orientan al estudio y descripción de las organizaciones como un sistema de relaciones ordenadas a diversos fines. Este análisis permite delinear el escenario formal que sirve como arena política para las acciones de los individuos en una organización, no obstante, se compone de explicaciones basadas exclusivamente en la racionalidad de las estructuras. Por ello, se suman los aportes de perspectiva cultural, en la cual se agrupan los resultados de trabajos centrados en los factores subjetivos de las organizaciones, tales como el clima de trabajo y las creencias y representaciones de los sujetos trabajadores. Finalmente, se agrega la perspectiva política que favorece la comprensión de los procesos micro-sociales y las formas de los intercambios políticos, como la distribución del poder y del liderazgo entre actores. Se concluye que un trabajo comparativo e integrador de ellas permite configurar categorías y esquemas interpretativos, integrando un modelo teórico plausible de ser aplicado al análisis de las universidades y sus problemas de gestión.","palabras_clave":"Cultura|Estructura|Política|Universidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63520,"idioma_id":4,"titulo":"N-terminal palmitoylation is required for Toxoplasma gondii HSP20 inner membrane complex localization","resumen":"Toxoplasma gondii is an obligate intracellular parasite and the causative agent of toxoplasmosis. Protein palmitoylation is known to play roles in signal transduction and in enhancing the hydrophobicity of proteins thus contributing to their membrane association. Global inhibition of protein palmitoylation has been shown to affect T. gondii physiology and invasion of the host cell. However, the proteins affected by this modification have been understudied. This paper shows that the small heat shock protein 20 from T. gondii (TgHSP20) is synthesized as a mature protein in the cytosol and is palmitoylated in three cysteine residues. However, its localization at the inner membrane complex (IMC) is dependent only on N-terminal palmitoylation. Absence or incomplete N-terminal palmitoylation causes TgHSP20 to partially accumulate in a membranous structure. Interestingly, TgHSP20 palmitoylation is not responsible for its interaction with the daughter cells IMCs. Together, our data describe the importance of palmitoylation in protein targeting to the IMC in T. gondii.&nbsp;","palabras_clave":"Toxoplasma|parasite|palmitoylation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63521,"idioma_id":4,"titulo":"Inhibition of Carbonic Anhydrase Prevents the Na+\/H+ Exchanger 1- dependent Slow Force Response to Rat Myocardial Stretch","resumen":"Myocardial stretch is an established signal that leads to hypertrophy. Myocardial stretch inducesa first immediate force increase followed by a slow force response (SFR), which is aconsequence of an increased Ca²+ transient that follows the NHE1 Na+\/H+ exchanger activation.Carbonic anhydrase II (CAII) binds to the extreme C-terminus of NHE1 and regulates itstransport activity. We aimed to test the role of CAII bound to NHE1 in the SFR. The SFR andchanges in intracellular pH (pHi) were evaluated in rat papillary muscle bathed with CO2\/HCO3-buffer and stretched from 92% to 98% of the muscle maximal force development length for 10min, in the presence of the CA inhibitor 6-ethoxzolamide (ETZ, 100 &#956;M). SFR control was120±3% (n=8) of the rapid initial phase and was fully blocked by ETZ, 99±4% (n=6). The SFRcorresponded with a maximal increase in pHi of 0.18±0.02 pH units (n=4), and pHi changes wereblocked by ETZ (0.04±0.04, n=6), as monitored by epifluorescence. NHE1\/CAII physicalassociation was examined in the SFR by coimmunoprecipitation, using muscle lysates. CAIIimmunoprecipitated with an anti-NHE1 antibody and the CAII immunoprecipitated proteinlevels increased 58±9% (n=6) upon stretch of muscles, assessed by immunoblots. The p90RSKkinase inhibitor SL0101-1 (10 &#956;M) blocked the SFR of heart muscles after stretch 102±2%(n=4), and reduced the binding of CAII to NHE1, suggesting that the stretch-inducedphosphorylation of NHE1 increases its binding to CAII. CAII\/NHE1 interaction constitutes acomponent of the SFR to heart muscle stretch which potentiates NHE1-mediated H+ transport inthe myocardium.","palabras_clave":"stretch|carbonic anhydrase II|slow force response"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63522,"idioma_id":4,"titulo":"Phenotypic plasticity in response to food source in Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae: Triatominae)","resumen":"In the Gran Chaco region of Argentina, Bolivia, and Paraguay, vector transmission of Trypanosoma cruzi, the etiological agent of Chagas disease, is still a severe problem because, among other causes, houses are reinfested with Triatoma infestans, the main vector of T. cruzi in southern South America. A better understanding of adaptation and evolution of T. infestans populations may contribute to the selection of appropriate vector control strategies in this region. Phenotypic plasticity is essential to understand development and maintenance of morphological variation. An experimental phenotypic plasticity study was conducted to assess if blood meal source induced head shape and size  variation during development in T. infestans. Eighteen full-sib families were assigned to one of two food sources (pigeon and guinea pig) to examine the effect of food source on head shape and size in all  nymph instars and adults. Data were analyzed using geometric morphometric tools and phenotypic plasticity analyses. Significant differences in head shape and size were observed between adults fed on different food sources. Allometric effects at the adult stage were observed. Head size showed significant food source × family interaction for fifth-instar nymphs and adults. For head size, significant differences between food sources were observed at stages and in ontogenetic trajectory. Phenotypic  plasticity expression was found for head shape and size in adults; indeed, bugs fed on guinea pigs exhibited greater changes in head shape and larger heads than those fed on pigeon. Full-sib families  exhibited different patterns of phenotypic expression in response to food source. Food source × family interaction may indicate that the observed variation in phenotypic plasticity may contribute to changes  in head morphometry. These results may contribute to the selection of an appropriate control strategy for T. infestans in the Gran Chaco region, since they provide evidences of morphological plasticity in this species.","palabras_clave":"Triatoma infestans|Phenotypic plasticity|Head morphometry|Food source"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63523,"idioma_id":4,"titulo":"Along-strike structural variations in the Southern Patagonian Andes: Insights from physical modeling","resumen":"The Southern Patagonian Andes between 48° and 53° SL offers a unique opportunity to study the results of orogenic growth superimposed over a previously rifted region. In this sector the northern Austral or Magallanes basin was affected by late to middle Jurassic extension followed by Late Cretaceous to Neogene tectonic compression. The resultant fold-thrust belt displays significant along-strike variations in width and lateral position of the structural domains that may be reflecting a first order control of the Jurassic extensional depocenters, as proposed in previous works. This hypothesis is tested using a series of scaled sandbox analog models involving the positive inversion of two adjacent depocenters with different extensional rates accommodated by a transfer fault. The correlation between structures in the Southern Patagonian Andes and the features in the model have important first order similarities supporting the theory of a positively inverted extensional basin with a previous history of southward increase in extension. Moreover structural cross-sections analyzed from field and seismic data have a geometry that is consistent with serial cross sections resulting from the models.","palabras_clave":"Southern Patagonian Andes|Asymmetric rift|Analog modeling|Tectonic inversion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63524,"idioma_id":4,"titulo":"The times they're a-changing: Effects of circadian desynchronization on physiology and disease","resumen":"Circadian rhythms are endogenous and need to be continuously entrained (synchronized) with the environment. Entrainment includes both coupling internal oscillators to external periodic changes as well as synchrony between the central clock and peripheral oscillators, which have been shown to exhibit different phases and resynchronization speed. Temporal desynchronization induces diverse physiological alterations that ultimately decrease quality of life and induces pathological situations. Indeed, there is a considerable amount of evidence regarding the deleterious effect of circadian dysfunction on overall health and on disease onset and progression, both in human studies and in animal models. In this review we discuss the general features of circadian entrainment and introduce diverse experimental models of desynchronization. In addition, we focus on metabolic, immune and cognitive alterations under situations of acute or chronic circadian desynchronization, as exemplified by jetlag and shiftwork schedules. Moreover, such situations might lead to an enhanced susceptibility to diverse cancer types. Possible interventions (including light exposure, scheduled timing for meals and use of chronobiotics) are also discussed.","palabras_clave":"CIRCADIAN RHYTHMS|ENTRAINMENT|JET-LAG|SHIFTWORK"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63525,"idioma_id":4,"titulo":"Assessment of sábalo (Prochilodus lineatus) fisheries in the lower Paraná River basin (Argentina) based on hydrological, biological, and fishery indicators","resumen":"This study assesses the change in fish exploitation patterns of the sábalo fisheries of the lower Paraná River basin based on  hydrological, biological, and fishery indicators. From historical catch records, we recorded a shift from a lightly and moderate  exploited status before 2001 to a heavily exploited one, as a result of the development of sábalo exports from 2001 onwards.  Channel and floodplain connectivity indices demonstrated that the Paraná exhibited highly favorable hydrological conditions  between 1972 and 1999 but more adverse conditions from 1999 to 2009, as a consequence of flood pulses reduction coincident  with a fishing mortality increment. A total catch of 15,000 tons, including local consumption, regional market, informal sales,  and exportation volumes, was estimated as the maximum sustainable catch. That level was instead exceeded 3 fold in 2004, in  parallel with a decrease in the mean fish length from 48 to 42 cm, along with a reduction in both the mesh size from 16 to 12 cm  and megaspawner proportion, and an increase of fishing mortality. Although growth overfishing was not noted, an analysis  of the spawning potential ratio trend indicated that recruitment overfishing took place during 2005, since the fishing mortality  rate was 4 times greater than the natural mortality rate. These observations suggest that less favorable hydrological conditions  after 1999 coupled with a coincident high fishing pressure could have impacted the sábalo population biomass. Management  of sábalo fisheries requires to set aside a single economic vision of the resource use moving to an ecosystem-oriented  approach that incorporate, among others components, the hydrological regime, species life history traits, fishing impacts on  other species, and main stakeholders socioeconomic requirements as key elements for the preservation of fishery sustainability.","palabras_clave":"Artisanal fisheries|Flood pulse|Neotropical rivers|Overfishing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63526,"idioma_id":4,"titulo":"Miocene block uplift and basin formation in the Patagonian foreland: The Gastre Basin, Argentina","resumen":"The intraplate fault-block mountains and intermontane deposits of the   Gastre Basin, which are recorded more than 550 km east of the Andean   trench in central Patagonia, Argentina, are analysed. The Gastre Basin   is one of the largest Patagonian intermontane basins, limited by   uplifted blocks strongly oblique to the Andean chain. It was originated   by reverse faulting and inversion of pre-existing normal faults   associated with a Mesozoic rift basin and defined by older crustal   heterogeneities. The deformational event occurred during the middle   Miocene, related to a short contractional episode (16.1?14.86 Ma),   probably in response to an eastward migration of the Andean fold and   thrust belt. During Pliocene to Quaternary times, neither younger   fault-block uplifts nor reconfigurations of the basin occurred.   Similarities between the study area and other parts of the Patagonian   foreland?such as the presence of Miocene reverse or inversion tectonics,   as well as the accommodation of the Miocene sedimentary   successions?suggest that the Gastre Basin is part of a major late early   to middle Miocene broken foreland system (i.e. the Patagonian broken   foreland) that exhumed discrete fault- block mountains and generated   contemporary basins along more than 950 km parallel to the Andean trench   (i.e. between 40°00&#8242; and 48°00&#8242; south latitude). Based on recent   studies on the southern Andean Margin, this continental-scale   contractional episode may be the result of a flat-slab subduction   segment. Nevertheless, such a hypothesis is very difficult to support   when analysing such a large flat subduction segment along the entire   Patagonian trench. This suggests the need to consider alternative   flat-slab trigger mechanisms or other factors in the generation of   broken foreland systems.","palabras_clave":"NEÓGENO|TECTONICA|CUENCAS SEDIMENTARIAS|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63527,"idioma_id":4,"titulo":"Late Cretaceous uplift in the Malargüe fold-and-thrust belt (35ªS) southern Central Andes of Argentina and Chile","resumen":"The Cordillera de los Andes is one of the most important mountain ranges in the world, extending for more than 8.000 km along the western margin of South America. It is the result of the subduction of the oceanic Nazca plate below continental South America, being the typical example of a subduction-related orogen (Dewey and Bird, 1970; James, 1971). The present configuration of the range is the result of Cenozoic deformation and uplift (Jordan et al., 1983; Mégard, 1984; Ramos and Alemán, 2000; Vicente, 2005; and many others). However, in many sectors of the Andes, the first stage of Andean uplift took place in the Late Cretaceous. This deformation stage is known as the Peruvian Phase, since it is more clearly recognized in the Peruvian Andes (Steinmann, 1929; Aubouin et al., 1973; Vicente et al., 1979; Mégard, 1984) where the deformation has been recently dated as starting in mid-Turonian times (Jaillard et al., 2005). Cretaceous compressional deformation has also been recognized in other sectors of the Andes such as the Northern Andes of Ecuador, Colombia and Venezuela (Aspden et al., 1992; Jaimes and de Freitas, 2006; Martin-Gombojav and Winkler, 2008), the Central Andes in Bolivia (Sempere, 1995), and the Patagonian Andes (Fildani et al., 2003). In the Southern Central Andes, south of 36ºS, deposits of the Late Cretaceous Neuquén Group have recently been interpreted as syn-orogenic strata corresponding to a Cretaceous foreland basin (Cobbold and Rossello, 2003; Ramos and Folguera, 2005). Cobbold and Rossello (2003) have also presented evidences of Late Cretaceous activity for many structures in the same area. Based on detrital zircon ages, Tunik et al. (2010) have dated the beginning of deposition of syn-orogenic strata as taking place in the early Cenomanian. At the latitude of 33ºS, Orts and Ramos (2006) have described syn-tectonic angular unconformities in beds of the Neuquén Group. In this contribution, we will present evidences of a Cretaceous age for the beginning of Andean uplift and deformation at 35ºS. ","palabras_clave":"Andes|orogeny|Cretaceous uplift|foreland basin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63528,"idioma_id":4,"titulo":"Alizarin complexone: An interesting ligand for designing TiO 2-hybrid nanostructures","resumen":"Herein we report the characterization and photoelectrochemical behavior of nanocrystalline TiO2 films modified with some alizarin derivatives. Particularly, alizarin complexone (3-[N,N-bis(carboxymethyl)-aminomethyl]-1,2- dihydroxyanthraquinone) and its Fe(ii) and Fe(iii) metal complexes have been examined. Based on UV-Visible and Raman spectroscopic studies we proposed that AC forms a tridentate complex with titanium dioxide involving the 2-OH and (methylimino) diacetate groups, while Fe(ii) and Fe(iii) AC-complexes bind to titanium dioxide through the carbonyl (CO) and hydroxyl (OH) groups at the 1,9 positions of the alizarin framework. These findings allow the design of different nanostructures with diverse photoelectrochemical behavior. Analysis of the j-V curves reveals a sign reversal at -90 mV and -100 mV upon the excitation of AC@TiO2 and [FeIIIAC]@TiO2 photoelectrodes, respectively, whereas [FeIIAC]@TiO2 displays only anodic photocurrents. A description involving both electronic and geometric factors to account for these differences is proposed.","palabras_clave":"alizarin complexone|photoelectrochemical|TiO2 films |Raman spectroscopic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63529,"idioma_id":4,"titulo":"Computational Analysis of a Vortex Ingesting Bioreactor for Hydrogen Production","resumen":"In this work, the fluid dynamics behaviour of a model bioreactor specifically designed for a fermentative hydrogen production process is investigated by Computational Fluid Dynamics (CFD) simulations. The  geometrical features of the bioreactor, which is a dual impellers baffled stirred tank provided with a draft tube and the gas-liquid characteristics of the vortex ingesting operating mode, make the modelling and the  numerical solution tasks particularly challenging. The computational strategy is based on the two-phase formulation of the Reynolds Averaged Navier-Stokes equations in an Eulerian framework for both the  continuous and the dispersed phase. The results of the simulations are compared with available experimental data under the same operating conditions and an identical bioreactor geometry. The  reliability of the predicted overall hydrodynamics behaviour and the accuracy of the turbulent two-phase mean velocity field are evaluated and critically discussed.","palabras_clave":"BIOHYDROGEN|VORTEX INGESTING|CFD|BIOREACTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63530,"idioma_id":4,"titulo":"Mathematical Programming and GAme Theory Optimization-based tool for Supply Chain Planning in Cooperative\/competitive Environments","resumen":"A multi-objective Mixed Integer Linear Programming (MILP) model is proposed in this paper, devised to optimize the tactical decisions of several Supply Chains (SC?s) with multiple objectives under uncertain competition behavior. Game Theory optimization has been used as a decision making tool, to deal with cooperative and\/or competitive scenarios. Three different optimization criteria are considered (total cost, total delivery time and expenses of the buyers for the competitive problem). The multi objective problem has been solved using the Pareto frontier solutions, and both cooperative and non cooperative scenarios are considered, so multiple optimization tools\/techniques (Game Theory, MILP based approach and Pareto frontiers) have been combined to improve the decision making, analyzing the different trade-offs associated to the policies related to planning problems under uncertainty. The model has been probed in a real multi-product, multi-echelon Supply Chain case study, based on the operation of two different SC?s in both competitive and cooperative situations.","palabras_clave":"SUPPLY CHAIN MANAGEMENT|MULTI-OBJECTIVE OPTIMIZATION|GAME THEORY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63531,"idioma_id":4,"titulo":"Biotechnological and in situ food production of polyols by lactic acid bacteria","resumen":"Polyols such as mannitol, erythritol, sorbitol, and  xylitol are naturally found in fruits and vegetables and are  produced by certain bacteria, fungi, yeasts, and algae. These  sugar alcohols are widely used in food and pharmaceutical  industries and in medicine because of their interesting physicochemical  properties. In the food industry, polyols are  employed as natural sweeteners applicable in light and  diabetic food products. In the last decade, biotechnological  production of polyols by lactic acid bacteria (LAB) has been  investigated as an alternative to their current industrial production.  While heterofermentative LAB may naturally produce  mannitol and erythritol under certain culture  conditions, sorbitol and xylitol have been only synthesized  through metabolic engineering processes. This review deals  with the spontaneous formation of mannitol and erythritol in  fermented foods and their biotechnological production by  heterofermentative LAB and briefly presented the metabolic  engineering processes applied for polyol formation.","palabras_clave":"LACTIC|ACID|BACTERIA|POLYOLS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63532,"idioma_id":4,"titulo":"NMDA receptor subunits in the adult rat hippocampus undergo similar changes after 5 minutes in an open field and after LTP induction","resumen":"NMDA receptor subunits change during development and their synaptic   expression is modified rapidly after synaptic plasticity induction in   hippocampal slices. However, there is scarce information on subunits   expression after synaptic plasticity induction or memory acquisition,   particularly in adults. GluN1, GluN2A and GluN2B NMDA receptor subunits   were assessed by western blot in 1) adult rats that had explored an open   field (OF) for 5 minutes, a time sufficient to induce habituation, 2)   mature rat hippocampal neuron cultures depolarized by KCl and 3)   hippocampal slices from adult rats where long term potentiation (LTP)   was induced by theta-burst stimulation (TBS). GluN1 and GluN2A, though   not GluN2B, were significantly higher 70 minutes--but not 30   minutes--after a 5 minutes session in an OF. GluN1 and GluN2A total   immunofluorescence and puncta in neurites increased in cultures, as   evaluated 70 minutes after KCl stimulation. Similar changes were found   in hippocampal slices 70 minutes after LTP induction. To start to   explore underlying mechanisms, hippocampal slices were treated either   with cycloheximide (a translation inhibitor) or actinomycin D (a   transcription inhibitor) during electrophysiological assays. It was   corroborated that translation was necessary for LTP induction and   expression. The rise in GluN1 depends on transcription and translation,   while the increase in GluN2A appears to mainly depend on translation,   though a contribution of some remaining transcriptional activity during   actinomycin D treatment could not be rouled out. LTP effective induction   was required for the subunits to increase. Although in the three models   same subunits suffered modifications in the same direction, within an   apparently similar temporal course, further investigation is required to   reveal if they are related processes and to find out whether they are   causally related with synaptic plasticity, learning and memory.","palabras_clave":"LTP|NMDA|Hippocampus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63533,"idioma_id":4,"titulo":"The type specimens of Tachinidae (Diptera) housed in the Museo Argentino de Ciencias Naturales \u201CBernardino Rivadavia\u201D, Buenos Aires","resumen":"The type material of species of Tachinidae (Diptera) housed in the collection of the Entomology Division of the Museo Argentino de CienciasNaturales \u201CBernardino Rivadavia\u201D were examined and are herein documented. The collection contains 202 type specimens consisting of 54species described by E.E. Blanchard and 12 described by J. Brèthes. Comparison of their original descriptions with the label information reveals the existence of 24 holotypes, 1 lectotype, 141 syntypes and 36 paratypes. Complete information is given for each type, including reference to the original description, label data, and preservation condition.","palabras_clave":"TAXONOMY|TACHINIDAE|MACN|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63534,"idioma_id":4,"titulo":"Forager abundance and its relationship with colony activity level in three species of South American Pogonomyrmex harvester ants","resumen":"The proportion of foragers in ant colonies is a fairly constant species-specific characteristic that could be determined by intrinsic or extrinsic factors. If intrinsic factors are relevant, species with similar life history characteristics (e.g., colony size and foraging strategies) would be expected to have a similar proportion of foragers in their colonies. Within the genus Pogonomyrmex, North American species can vary largely in their colony size, whereas only species with small colonies are known in South America.We studied the characteristics of the foraging subcaste in three sympatric South American species of Pogonomyrmex harvester ants, and compared it with the available information on other species of the same genus. We used two mark-recapture methods and colony excavations to estimate the number and proportion of foragers in the colonies of P. mendozanus, P. inermis, and P. rastratus, and to test the relationship between forager external activity levels and abundance per colony. Forager abundance in the three studied species was lower than in most North American species. The percentage of foragers in their colonies ranged 7-15 %, more similar to North American species with large colonies than to those with small colony size. Foraging activity was positively correlated with forager abundance in all three species, implying that colony allocation to number of foragers allows for higher food acquisition. Further comparative studies involving a wider range of traits in South and North American species would allow to unveil the role of environmental factors in shaping each species' particular traits.","palabras_clave":"FORAGER ABUNDANCE|COLONY SIZE|POGONOMYRMEX|FORAGING ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63535,"idioma_id":4,"titulo":"Effect of drought on morphological and functional traits of Poa ligularis and Pappostipa speciosa, native perennial grasses with wide distribution in Patagonian rangelands, Argentina","resumen":"Poa ligularis Nees. Ap. Steudel and Pappostipa speciosa (Trin. et Rupr.) Romaschenko are dominant perennial grasses in the arid Patagonian rangelands of Argentina. Both species are exposed to periods of water shortage during spring-summer and are grazed by domestic and native herbivores. Pappostipa speciosa displays xeromorphic adaptations and is less preferred by herbivores than P. ligularis. The knowledge of how drought affects morphological\/functional traits in coexisting perennial grass species is useful to understanding the function of desert perennial grasses, and for the use and conservation of Patagonian arid rangelands. The hypothesis of this study was that co-existing perennial grasses contrasting in drought resistance mechanisms display different degrees of phenotypic plasticity in underlying and\/or functional traits. Plants of both species were exposed to two levels of gravimetric soil moisture: 16% (~field capacity) and 4%. Plant vegetative and reproductive traits were measured weekly in individual plants and these were harvested at the end of the experiment. Aboveground and root biomass were separated in the harvested plants and the concentration of photosynthetic pigments wasassessed in green leaves. The trait response range was also calculated through the plasticity index. In both species, drought stress led to significant reductions in plant height, total plant dry weight, number of total leaves, dry weight of green and senescent leaf, percentage of flowering plants, length of inflorescences, and number, length and dry weight of roots. The concentration of photosynthetic pigments increased under drought in both species. In conclusion, drought strongly affectedreproductive and vegetative traits in both species and the greatest negative effect of drought was found in P. speciosa, the most conservative species. However, our findings might indicate that both species are able to maintain photosynthetic activity through the increase of photosynthetic pigments under drought conditions in Patagonian rangelands.","palabras_clave":"DROUGHT|POA LIGULARIS|PAPPOSTIPA SPECIOSA|PLASTICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63536,"idioma_id":4,"titulo":"Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas","resumen":"Meningiomas, the most frequent benign intracranialand intraspinal types of tumors are normallyremoved by surgery. Complications can occur when thetumor is critically localized and cannot be completelyremoved or when comorbidities of the mostly elder patientsincrease the general surgical risk. Thus, alternate medicaltreatment concepts for the therapy of meningiomas wouldbe desirable. Curcumin, the active ingredient of the spiceplant Curcuma longa has shown anti-tumorigenic actionsin many different types of tumors and therefore, its effecton growth and apoptosis of meningioma cells was studiedin the present paper. In vitro, treatment of the human Ben-Men-1 meningioma cell line and of a series of 21 primaryhuman meningioma cell cultures with curcumin (1\u201320 lM)strongly reduced the proliferation in all cases in a dosedependent manner. Cell cycle analysis by fluorescenceactivatedcell sorting showed growth arrest at G2\/M phase,which was confirmed by demonstrating the correspondingmodulation of proteins involved in G2\/M arrest byimmunoblotting and\/or confocal laser microscopy. Highdosages (20, 50 lM) of curcumin induced a significantincrease of apoptosis in Ben-Men-1 and primary meningiomacell cultures as demonstrated by morphologicalchanges of cell nuclei, DNA fragmentation, translocationof cell membrane associated phosphatidyl serine and theinduction of apoptotic-acting cleaved caspase-3. Ourresults suggest that the multi-targeting drug curcumin haspotent anti-tumorigenic actions in meningioma cells andmight therefore be a putative candidate for the pharmacologicaltreatment of meningiomas.","palabras_clave":"Meningioma|Curcumina|Proliferation|Apoptosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63537,"idioma_id":4,"titulo":"Oocyte maturation and in vitro hormone production in small antral follicles (SAFs) isolated from rhesus monkeys","resumen":"Purpose: The small antral follicles (SAFs) from the ovarian medulla can be a potential source of oocytes for infertility patients, but little is known about their ability to yield mature oocytes. This study evaluated the response of these SAFs to a stimulatory bolus of human corionic gonadotropin (hCG) in vitro. Methods: Oocyte nuclear maturation and hormone production (estradiol [E2], progesterone [P4]), antimullerian hormone [AMH]) by individual intact SAFs (n=91; &gt;0.5 mm; n=5 monkeys) was evaluated after 34 hr of culture in the absence (control) or presence of hCG. Results: Of the total cohort (n=91), 49% of SAFs contained degenerating oocytes. The percentage of healthy oocytes able to reinitiate meiosis to the metaphase I (MI) and MII was greater (p&lt;0.05) after hCG compared to controls. E2, P4 and AMH levels were higher (p&lt;0.05) in SAF cultures containing germinal vesicle (GV) oocytes compared to those with MII oocytes regardless of hCG exposure. SAF with MI oocytes produced more E2, but less (p&lt;0.05) P4 and AMH compared to SAFs containing GV oocytes (p&lt;0.05). Follicles ¡Ý 1mm produced more (p&lt;0.05) E2, whereas follicle diameter did not correlate with P4 or AMH levels. Only P4 increased (p&lt;0.05) in response to hCG, regardless of follicle size or oocyte maturity. SAFs containing degenerating oocytes produced similar levels of E2, P4 and AMH compared to SAFs containing healthy oocytes. Conclusions: These data indicate, for the first time, that oocytes within primate SAFs can reinitiate meiosis in vitro in the absence of hCG, but nuclear maturation is enhanced in SAFs cultured with hCG. Oocyte nuclear maturation within SAFs in is associated with decreased E2, P4 and AMH levels. Furthermore, hormone content within the culture media does not necessarily reflect oocyte quality. ","palabras_clave":"SMALL ANTRAL FOLLICLE|OOCYTE MATURATION|RHESUS MONKEY|E2|P4|AMH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63538,"idioma_id":4,"titulo":"Evidence of heterogeneous selection on quantitative traits of Prosopis flexuosa (Leguminosae) from multivariate QST?FST test","resumen":"Prosopis flexuosa is an arboreal Leguminosae that grows in arid and semiarid temperate zones of Argentina, in the Monte eco-region. It is a promising native forest species for recovering arid and semiarid regions because it plays an important role in erosion control as well as in soil fertility. Furthermore, it provides diverse economical resources. The main challenge to the forestry sector is finding a balance between production and forest protection. For this purpose, it is necessary to gather information about genetic parameters. In this study, we measured the distribution of the variation of 14 quantitative traits in an experimental half-sib stand, where families are representative of hierarchically structured populations. We applied a multivariate extension of the classical QST?FST neutrality test to determine the relative importance of drift versus selection in the distribution of genetic variability. We found strong evidence that different selective regimes act on different traits and that selection favors different optima in each sampling site. The selection to different optima is much stronger among than within provenances. This result helps explain the possible causes for the regional variation observed in P. flexuosa and to define the management units and the evolutionarily significant units for this species.","palabras_clave":"Multivariate QST  |Quantitative traits|Molecular  markers |FST|Prosopis flexuosa|Forest trees |Selection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63539,"idioma_id":4,"titulo":"The carbohydrate-binding module of Fragaria x ananassa expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities 'in vitro'..","resumen":"A putativecarbohydrate binding module (CBM) from strawberry (Fragaria x ananassa Duch.) expansin 2 (CBM-FaExp2) was cloned andthe encoding protein was over-expressed in Escherichiacoli and purified in order to evaluate its capacity to bind different cellwall polysaccharides \u201Cin vitro\u201D. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectinwith different affinities (Kad= 33.6 mL g-1 ± 0.44; Kad=11.37 mL g-1 ± 0.87; Kad= 10.4 mL g-1 ± 0.19,respectively). According to \u201Cin vitro\u201D enzyme assays, this CBM isable to decrease the activity of cell wall degrading enzymes such aspolygalacturonase, endo-glucanase, pectinase and xylanase, probably because thebinding of CBM-FaExp2 to the different substrates interferes with enzymeactivity. The results suggest that expansins would bind not only cellulose butalso a wide range of cell wall polymers. ","palabras_clave":"CBM|Cell Wall|Expansin|Strawberry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63540,"idioma_id":4,"titulo":"PPAR activation as a regulator of lipid metabolism, nitric oxide production and lipid peroxidation in the placenta from type 2 diabetic patients","resumen":"PPARs (Peroxisome Proliferator Activated Receptors) are ligand activated transcription factors with crucial functions in lipid homeostasis, anti-inflammatory processes and placental development. Maternal diabetes induce a pro-inflammatory environment and alter placental development. We investigated whether PPARs regulate lipid metabolism and nitric oxide (NO) production in placentas from healthy and type 2 diabetic (DM2) patients. We found decreased PPARalpha and PPARgamma concentrations, no changes in PPARdelta concentrations, as well as increased lipids, lipoperoxides and NO production in placentas from DM2 patients. Activation of PPARalpha and PPARdelta reduced the placental lipid concentrations of phospholipids, cholesteryl esters, triglycerides and cholesterol. PPARgamma agonists increased lipid concentrations in placentas from DM2 patients and more markedly in placentas from healthy patients. Endogenous ligands from the three PPAR isotypes reduced NO production and lipoperoxidation in placentas from DM2 patients. We conclude that PPARs play a role in placental lipid metabolic and anti-oxidant\/anti-inflammatory pathways, relevant to regulate impaired lipid homeostasis and a pro-oxidant\/pro-inflammatory environment in placentas from DM2 patients.","palabras_clave":"Diabetes|Gestation|PPARs|Placenta"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63541,"idioma_id":4,"titulo":"Ethanol production from corn contaminated with fumonisins: a preliminary economic analysis including novel processing alternatives","resumen":"In this work, technical and economical feasibility of bioethanol production from corn with high concentrations of fumonisins is analyzed. Based on data obtained from a limited number of experiments, cost data of ethanol facilities and conceptual design methods maximum prices for corn contaminated with fumonisins are estimated. The scope of the analysis includes average ethanol concentrations in the fermentor in a 6-3 wt. % range for non contaminated corn and strongly contaminated corn (1400 ppm), respectively. The maximum price for contaminated corn varies from 66% to 33% of the fumonisins-free feedstock cost according to the level of contamination.                 The performance of the continuously operated process was also analyzed considering the coupling of the fermentor with a pervaporation unit for continuous ethanol separation. Estimations were made for a volumetric productivity of alcohol of 7.8 kg\/(m3h) and membrane flux (0.9 kg\/( m2h)) and selectivity (S= 5) corresponding to a commercial PDMS membrane for a level of 6 wt.% ethanol in the stirred-tank fermentor. Results show that an increase of 100 % in the membrane flux with a constant value for the selectivity is required to make the continuous alternative attractive.               ","palabras_clave":"BIOETHANOL|HYBRID|PROCESS|PERVAPORATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63542,"idioma_id":5,"titulo":"La adicción a la nicotina: vulnerabilidad, epigénesis y modelos animales de estudio","resumen":"Nicotine addiction: studies about vulnerability, epigenesis and animal models: This article is a summary about the current research of nicotine effects on the nervous system and its relationship to the generation of an addictive behavior. Like other drugs of abuse, nicotine activates the reward pathway, which in turn is involved in certain psychiatric diseases. There are individuals who have a high vulnerability to nicotine addiction. This may be due to genetic and epigenetic factors and\/or the environment. In this review, we described some epigenetic factors that may be involved in those phenomena. The two animal models most widely used for studying the reinforcing effects of nicotine are: self-administration and conditioning place preference (CPP). Here, we emphasized the CPP, due to its potential application in humans. In addition, we described the locomotor activity model (as a measure of psychostimulant effects) to study vulnerability to drugs of abuse.","palabras_clave":"NICOTINA|CONDICIONAMIENTO|EPIGENESIS|ADICCION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63543,"idioma_id":4,"titulo":"The snout of Cricosaurus araucanensis: a case study in novel","resumen":"  Metriorhynchids are the only crocodyliforms adapted to pelagic marine life. Snout  natural endocasts of the Tithonian (Late Jurassic) metriorhynchid Cricosaurus araucanensisCricosaurus araucanensis   indicated that skeletal changes defining the peculiar metriorhynchid body  plan were coupled with changes of the soft cephalic anatomy such as the enlarged salt  glands and restructuring of the paranasal sinus system. Seven new natural endocasts of  the snout and a 3-D reconstruction of C. araucanensis are described. Data from these   casts and the reconstruction are congruent, and they are combined into an accurate  reconstruction that improves our knowledge of the pre-orbital anatomy. The olfactory  tract, bulbs, olfactory nasal region and the anterior extension of the antorbital sinus  within the maxilla are recognized. Osteological correlates of the salt gland body are  also proposed. Palaeobiological inferences are erected based on the integration of  natural endocasts and 3-D reconstruction data. It is proposed that C. araucanensis  casts and the reconstruction are congruent, and they are combined into an accurate  reconstruction that improves our knowledge of the pre-orbital anatomy. The olfactory  tract, bulbs, olfactory nasal region and the anterior extension of the antorbital sinus  within the maxilla are recognized. Osteological correlates of the salt gland body are  also proposed. Palaeobiological inferences are erected based on the integration of  natural endocasts and 3-D reconstruction data. It is proposed that C. araucanensis    casts and the reconstruction are congruent, and they are combined into an accurate  reconstruction that improves our knowledge of the pre-orbital anatomy. The olfactory  tract, bulbs, olfactory nasal region and the anterior extension of the antorbital sinus  within the maxilla are recognized. Osteological correlates of the salt gland body are  also proposed. Palaeobiological inferences are erected based on the integration of  natural endocasts and 3-D reconstruction data. It is proposed that C. araucanensis  C. araucanensis are described. Data from these   casts and the reconstruction are congruent, and they are combined into an accurate  reconstruction that improves our knowledge of the pre-orbital anatomy. The olfactory  tract, bulbs, olfactory nasal region and the anterior extension of the antorbital sinus  within the maxilla are recognized. Osteological correlates of the salt gland body are  also proposed. Palaeobiological inferences are erected based on the integration of  natural endocasts and 3-D reconstruction data. It is proposed that C. araucanensisC. araucanensis   nasal salt glands were highly vascularized with a blood supply comparable with those  of extant marine birds. Reduced olfactory bulbs and olfactory nasal region indicate  that the aerial olfaction, differing from extant crocodilians, was not well  developed.","palabras_clave":"Metriorhynchidae|Natural and digital endocasts|Olfactory region|Paranasal sinuses|Thalattosuchia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63544,"idioma_id":4,"titulo":"Experimental hypothyroidism increases apoptosis in dimethylbenzanthracene- induced mammary tumors","resumen":"Epidemiological and in vitro data have not provided conclusive evidence concerning the involvement of thyroid hormones (THs) on mammary carcinogenesis. We used an in vivo model to assess the relationship between THs, adipose tissue and breast cancer development. Female Sprague&#8209;Dawley rats were treated with a dose of 7,12-dimethylbenz(a)anthracene (15 mg\/rat) at 55 days of age and were then divided into four experimental groups: hypothyroid rats (HypoT, 0.01% 6-N-propyl-2-thiouracil in drinking water), untreated control (EUT); hyperthyroid rats (HyperT, 0.25 mg\/kg\/day T4 s.c.) and vehicle-treated control rats. The latency of tumor appearance and the incidence and progression of tumors were determined. At sacrifice, blood samples were collected for hormone determinations and samples of tumor and mammary glands were obtained for immunohistological studies. HypoT rats had retarded growth and an increase in mammary fat. The latency was longer (p&lt;0.0001), the incidence rate was lower (p&lt;0.05) and tumor growth was slower in HypoT rats compared to EUT and HyperT rats. Mitotic index and PCNA immunostaining were similar in all groups. HypoT rats showed increased apoptosis (p&lt;0.05) as evaluated by the apoptotic index and TUNEL staining. No differences in serum prolactin and progesterone were observed. However, circulating estradiol (E2) was significantly lower in HypoT and HyperT rats. Serum leptin levels were reduced in HypoT rats even though the abdominal fat mass was similar in all groups. To note, the leptin level was higher in HypoT rats that developed mammary tumors than the level in non-tumoral HypoT rats. In conclusion, hypothyroidism altered animal growth, breast morphology, body composition and leptin secretion and serum E2 enhancing apoptosis and, consequently, retarding mammary carcinogenesis in rats.","palabras_clave":"HYPOTHYROIDISM |APOPTOSIS|DIMETHYLBENZANTHRACEN|MAMMARY TUMORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63545,"idioma_id":4,"titulo":"Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina","resumen":"Abstract  Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1\/P7 and R16F2n\/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n\/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina.","palabras_clave":"ARGENTINA|PEACH|YELLOWING|PCR-RFLP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63546,"idioma_id":4,"titulo":"Astrocytes: new targets of melanocortin 4 receptor actions","resumen":"Astrocytes exert a wide variety of functions with paramount importance in brain physiology. After injury or infection, astrocytes become reactive and they respond by producing a variety of inflammatory mediators that help maintain brain homeostasis. Loss of astrocyte functions as well as their excessive activation can contribute to disease processes thus it is important to modulate reactive astrocyte response. Melanocortins are peptides with well-recognized anti-inflammatory and neuroprotective activity. Although melanocortin efficacy was shown in systemic models of inflammatory disease, mechanisms involved in their effects have not yet been fully elucidated. Central anti-inflammatory effects of melanocortins and their mechanisms are even less well known and in particular the effects of melanocortins on glial cells are poorly understood. Of the five known melanocortin receptors (MCR) only subtype 4 is present in astrocytes. MC4R has been shown to mediate melanocortin effects on energy homeostasis, reproduction, inflammation and neuroprotection and, recently, to modulate astrocyte functions. In this review we will describe MC4R involvement in anti-inflammatory, anorexigenic, and anti-apoptotic effects of melanocortins on the brain. We will highlight MC4R action in astrocytes and discuss their possible mechanisms of action. Melanocortin effects on astrocytes provide a new means of treating inflammation, obesity, and neurodegeneration, making them attractive targets for therapeutic interventions in the central nervous system","palabras_clave":"MELANOCORTIN 4 RECEPTOR|ASTROCYTES|NEUROPROTECTION|NEUROINFLAMMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63547,"idioma_id":4,"titulo":"Understanding species persistence for defining conservation actions: a management landscape for jaguars in the Atlantic Forest","resumen":"Habitat models constitute useful instruments for understanding species-habitat interactions and can constitute helpful conservation tools. The Upper Paraná Atlantic Forest (UPAF) of South America still holds the world´s southernmost jaguar (Panthera onca) population. Our aims were (i) to test several a priori hypotheses on the factors affecting jaguar persistence in this region, (ii) to map habitat suitability and identify areas with potentially conflicting habitat conditions, and (iii) to identify priority areas for management and improve the conservation initiatives for jaguars and the UPAF. Following an information-theoretic approach, we used presence records of jaguars and pseudoabsences in generalized linear models. We structured hypotheses into two groups which demand different management actions: land cover and human persecution. The best model of each group was used to develop a two-dimensional habitat model. Jaguar persistence was favoured by current and historical native forest cover, and hindered by human land uses. Protection favoured jaguar presence whereas human accessibility and high human population density had negative effects. The twodimensional model suggests that <8% (20 670 km2) of the landscape represents potential core areas for jaguars (good land-cover characteristics and low human persecution) and 11.8% (32 563 km2) stands as potentially attractive sinks where good land-cover conditions conflict with high human persecution. Reduction of human persecution is urgently needed to increase the core areas for jaguars in this region, but improvement of land-cover conditions is important for sustaining the connectivity among jaguar populations that seem to be isolated in different areas of the UPAF.","palabras_clave":"Attractive sink;|Human persecution|Land-cover conditions|Panthera onca|Two-dimensional habitat model|Upper Paraná Atlantic Forest"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63548,"idioma_id":4,"titulo":"The Ultraviolet Radiation Environment around M dwarf Exoplanet Host Stars","resumen":"The spectral and temporal behavior of exoplanet host stars is a critical   input to models of the chemistry and evolution of planetary   atmospheres. Ultraviolet photons influence the atmospheric temperature   profiles and production of potential biomarkers on Earth-like planets   around these stars. At present, little observational or theoretical   basis exists for understanding the ultraviolet spectra of M dwarfs,   despite their critical importance to predicting and interpreting the   spectra of potentially habitable planets as they are obtained in the   coming decades. Using observations from the Hubble Space Telescope,   we present a study of the UV radiation fields around nearby M dwarf   planet hosts that covers both far-UV (FUV) and near-UV (NUV)   wavelengths. The combined FUV+NUV spectra are publicly available in   machine-readable format. We find that all six exoplanet host stars in   our sample (GJ&nbsp;581, GJ&nbsp;876, GJ&nbsp;436, GJ&nbsp;832, GJ&nbsp;667C, and GJ&nbsp;1214)   exhibit some level of chromospheric and transition region UV emission.   No 'UV-quiet' M dwarfs are observed. The bright stellar Ly&#945; emission   lines are reconstructed, and we find that the Ly&#945; line fluxes comprise   ~37%-75% of the total 1150-3100 Å flux from most M dwarfs; 103 times the solar value. We develop an empirical scaling relation between Ly&#945; and Mg II emission, to be used when interstellar H I attenuation precludes the direct observation of Ly&#945;. The intrinsic unreddened flux ratio is F(Ly&#945;)\/F(Mg II) = 10 ± 3. The F(FUV)\/F(NUV) flux ratio, a driver for abiotic production of the suggested biomarkers O2 and O3, is shown to be ~0.5-3 for all M dwarfs in our sample, &gt;103   times the solar ratio. For the four stars with moderate signal-to-noise   Cosmic Origins Spectrograph time-resolved spectra, we find UV emission   line variability with amplitudes of 50%-500% on 102-103 s timescales. This effect should be taken into account in future UV transiting planet studies, including searches for O3 on Earth-like planets. Finally, we observe relatively bright H2   fluorescent emission from four of the M dwarf exoplanetary systems   (GJ&nbsp;581, GJ&nbsp;876, GJ&nbsp;436, and GJ&nbsp;832). Additional modeling work is needed   to differentiate between a stellar photospheric or possible   exoplanetary origin for the hot (T(H2)  2000-4000&nbsp;K) molecular gas observed in these objects.","palabras_clave":"PLANETARY SYSTEMS|STARS: ACTIVITY|STARS: LOW MASS|STARS: ULTRAVIOLET"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63549,"idioma_id":4,"titulo":"Magnetic Activity Cycles in the Exoplanet Host Star epsilon Eridani","resumen":"The active K2 dwarf Eri has been extensively characterized both as a young solar analog and more recently as an exoplanet host star. As one of the nearest and brightest stars in the sky, it provides an unparalleled opportunity to constrain stellar dynamo theory beyond the Sun. We confirm and document the 3-year magnetic activity cycle in eps Eri originally reported by Hatzes and coworkers, and we examine the archival data from previous observations spanning 45 years. The data show coexisting 3-year and 13-year periods leading into a broad activity minimum  that resembles a Maunder minimum-like state, followed by the resurgence of a coherent 3-year cycle. The nearly continuous activity record suggests the simultaneous operation of two stellar dynamos with cycle periods of 2.95 ± 0.03 years and 12.7 ± 0.3 years, which, by analogy with the solar case, suggests a revised identification of the dynamo mechanisms that are responsible for the so-called 'active' and 'inactive' sequences as proposed by Bohm-Vitense. Finally, based on the observed properties of Eri, we argue that the rotational history of the Sun is what makes it an outlier in the context of magnetic cycles observed in other stars (as also suggested by its Li depletion), and that a Jovian-mass companion cannot be the universal explanation for the solar peculiarities.","palabras_clave":"Stars: activity|Stars: chromospheres|Stars: individual(HD 22049)|Surveys"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63550,"idioma_id":4,"titulo":"A time and space correlated turbulence synthesis method for Large Eddy Simulations","resumen":"In the present work the problem of generating synthesized turbulence at inflow boundaries of the simulation domain is addresed in the context of the Large Eddy Simulation (LES) method. To represent adequately certain statistical properties of a turbulent process, we propose a synthesized turbulence method which is based on previous works [Huang,Smirnov]. For this purpose, time and space correlations are introduced strictly in the mathematical formulation of the synthetic turbulence inflow data. It is demonstrated that the proposed approach inherits the properties of the methods on which it is based while presents some particular advantages as well.  The strategy of imposing conditions on the inlet velocity field through turbulence synthesis is  implemented in the parallel multiphysics code called PETSc-FEM (http:\/\/www.cimec.org.ar\/petscfem) primarily targeted to calculations throughout finite elements on general unstructured 2D and 3D grids. We present several numerical tests in order to validate and evaluate the method describing the dynamic phenomena that take place in real-life problems, such as a swirling turbulent flow inside a diffuser and the airflow around a vehicle model inside a wind tunnel at high Reynolds number.","palabras_clave":"Turbulence|Synthesis of inlet boundary conditions|Time and Space Correlation|Numerical modeling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63551,"idioma_id":4,"titulo":"Urodilatin regulates renal dopamine metabolism","resumen":"Background: Sodium and water transport across renal proximal tubules are  regulated by diverse hormones such as dopamine and urodilatin. We have  previously reported that urodilatin stimulates extraneuronal dopamine uptake  in external renal cortex by activation of the type A natriuretic peptide receptor,  coupled to cyclic guanylate monophosphate signal and protein kinase G.  Moreover, urodilatin enhances dopamine-induced inhibition of Na+, K+-  ATPase activity in renal tubules. The aim of the present study was to evaluate  whether urodilatin could also alter renal dopamine synthesis, release,  catabolism and turnover.  Methods: The effects of urodilatin on dopamine synthesis, release,  catabolism and turnover were measured in samples of renal cortex from  Sprague-Dawley rats.  Results: The results indicate that urodilatin increases L-dopa decarboxylase  activity and decreases catechol-o-methyl transferase and monoamine  oxidase activity. Moreover, urodilatin does not affect either dopamine basal  secretion or potassium chloride-induced dopamine release in external renal  cortex, and reduces amine turnover.  Conclusions: Both the present results and previous findings show that  urodilatin modifies dopamine metabolism in external renal cortex of rats by  enhancing dopamine uptake and synthesis and by decreasing catechol-omethyl  transferase and monoamine oxidase activity and dopamine turnover.  Those effects taken together may favor dopamine accumulation in renal cells  3  and increase its endogenous content and availability. This would permit D1  receptor recruitment and stimulation and, in turn, Na+, K+-ATPase activity  over inhibition that results in decreased sodium reabsorption. Therefore,  urodilatin and dopamine enhance natriuresis and diuresis through a common  pathway.","palabras_clave":"URODILATIN|DOPAMINE|KIDNEY|METABOLISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63552,"idioma_id":4,"titulo":"Ab initio and shell model studies of structural, thermoelastic and vibrational properties of SnO 2 under pressure","resumen":"The pressure dependences of the structural, thermoelastic and vibrational properties of SnO2 in its rutile phase are studied, as well as the pressure-induced transition to a CaCl2-type phase. These studies have been performed by means of ab initio (AI) density functional theory calculations using the localized basis code SIESTA. The results are employed to develop a shell model (SM) for application in future studies of nanostructured SnO2. A good agreement of the SM results for the pressure dependences of the above properties with the ones obtained from present and previous AI calculations as well as from experiments is achieved. The transition is characterized by a rotation of the Sn-centered oxygen octahedra around the tetragonal axis through the Sn. This rotation breaks the tetragonal symmetry of the lattice and an orthorhombic distortion appears above the critical pressure Pc. A zone-center phonon of B1g symmetry in the rutile phase involves such rotation and softens on approaching Pc. It becomes an Ag mode which stabilizes with increasing pressure in the CaCl2 phase. This  behavior, together with the softening of the shear modulus (C11 &#8722;C12)\/2 related to the orthorhombic distortion, allows a precise determination of a value for Pc. An additional determination is provided by the splitting of the basal plane lattice parameters. Both the AI and the experimentally observed softening of the B1g mode are incomplete, indicating a small discontinuity at the transition. However, all results show continuous changes in volume and lattice parameters, indicating a second-order transition. All these results indicate that there should be sufficient confidence for the future employment of the shell model.","palabras_clave":"SnO2|nanoparticles|Shell model"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63553,"idioma_id":4,"titulo":"Paleoenvironmental studies of Alfar archaeological site (mid-Holocene; Southeastern Pampas of Argentina): Silicophytoliths, gastropods and archaeofauna","resumen":"The firsts results of the silicophytolith research carried out for the sedimentary sequence of Alfar archaeological site are presented. The aims of this study were to identify the vegetation associations and to contribute to the reconstruction of past environments during the hunter-gatherers? occupation of the site in the mid-Holocene. This siliceous microfossil data is also complemented with other proxy evidence  derived from the freshwater gastropods recorded throughout the stratigraphic sequence and the zooarchaeological study of the bone remains recovered from the site. The results of this multi-proxy  approach show the existence of a permanent cover of gramineous communities on a lagoon margin and a considerable pedogenetic activity during human occupation. The obtained data indicates that the  human populations occupied Alfar site under dry conditions, but with the warm climate of the mid-Holocene Hypsithermal.","palabras_clave":"no se requieren"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63554,"idioma_id":4,"titulo":"FAS-DPD (FAmily Specific Degenerate Primer Design): A tool to design consensus degenerated oligonucleotides","resumen":"Se presenta el desarrollo de un algoritmo para el diseño de primers universales que incluyen degeneraciones","palabras_clave":"degenerated primer design|universal primers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63555,"idioma_id":4,"titulo":"Structural and magnetic behavior of ferrogels obtained by freezing thawing of polyvinyl alcohol\/poly (acrylic acid) (PAA)-coated iron oxide nanoparticles","resumen":"Superparamagnetic ferrogels with high swelling ability and potential   applications as solvent absorbers and stimuli-responsive drug delivery   devices were obtained by a non-toxic and environmentally friendly route   based on dispersion of poly(acrylic acid)-coated iron oxide   nanoparticles (PAA-coated NPs) in poly(vinyl alcohol) (PVA) solutions   followed by freezing-thawing. Presence of carboxylate groups arising   from the PAA coating allowed hydrogen bonding formation between NPs and   PVA and enabled the synthesis of optically homogenous, superparamagnetic   materials formed by a homogenous distribution of NPs diffuse clusters   in the PVA matrix. The addition of PAA-coated NPs produced a remarkable   increase in crystallinity degree, thermal degradation and swelling   percentage respect to the neat matrix, which demonstrates that ferrogels   with improved properties can be obtained by this procedure. Thereafter,   combination of a cryogenic technique with the use of non-toxic   components and magnetic NPs coated by a pH sensitive polymer makes these   ferrogels very promising for applications in the biomedical field.","palabras_clave":"Magnetic nanoparticles|Ferrogel|Magnetic properties|Iron oxides"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63556,"idioma_id":4,"titulo":"Optimal bundles in normed spaces","resumen":"In this paper, we prove existence of optimal bundles for a countable set of data in a broad class of normed spaces, which extend previous known results for a finite data set in a Hilbert space. In addition, we study the behavior of deviations and diameters for an increasing sequence of data  sets.","palabras_clave":"OPTIMAL BUNDLES|DEVIATION|DIAMETER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63557,"idioma_id":4,"titulo":"The Parkinson-associated human P5B-ATPase ATP13A2 increases the spermidine uptake","resumen":"P-type ion pumps are membrane transporters that have been classified into five subfamilies termed P1-P5. The ion transported by the P5-ATPases is not known. Five genes named ATP13A1-ATP13A5 that belong to the P5-ATPase group have been identified in humans. Mutations of the human gene ATP13A2 underlie a form of Parkinson?s disease (PD). Previous studies have suggested a relation between polyamines and P5B-ATPases. We have recently shown that the cytotoxicity induced by the polyamine analog paraquat (1,1´-dimethyl-4,4´-bipyridinium), which is an environmental agent related to PD development, was increased in ATP13A2-expressing CHO cells.    Here we show that ATP13A2-expressing CHO cells exhibit two-fold higher accumulation of spermidine. Increasing concentrations of spermidine reduced the viability of CHO cells stably expressing the ATP13A2. The higher levels of spermidine attained by the ATP13A2-expressing CHO cells were correlated with the increase in the ATP-dependent spermidine uptake in an isolated subcellular fraction containing lysosomes and late endosomes. These results support the idea that the human P5B-ATPase ATP13A2 is involved in polyamine uptake.","palabras_clave":"Parkinson|ATP13a2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63558,"idioma_id":4,"titulo":"On the role of ATP release, ectoATPase activity and extracellular ADP in the regulatory volume decrease of Huh-7 human hepatoma cells","resumen":"Hypotonicity triggered in human hepatoma cells (Huh-7) the release of ATP and cell swelling, followed by volume regulatory decrease (RVD). We analyzed how the interaction between those processes modulates cell  volume. Cells exposed to hypotonic medium swelled 1.5 times their  basal volume. Swelling was followed by 41% RVD40 (extent of RVD  after 40 min of maximum), whereas the concentration of extracellular  ATP (ATPe) increased 10 times to a maximum value at 15 min.  Exogenous apyrase (which removes di- and trinucleotides) did not  alter RVD, whereas exogenous Na-K-ATPase (which converts  ATP to ADP in the extracellular medium) enhanced RVD40 by 2.6  times, suggesting that hypotonic treatment alone produced a basal  RVD, whereas extracellular ADP activated RVD to achieve complete  volume regulation (i.e., RVD40 100%). Under hypotonicity, addition of 2-(methylthio)adenosine 5=-diphosphate (2MetSADP; ADP  analog) increased RVD to the same extent as exposure to Na-K-  ATPase and the same analog did not stimulate RVD when coincubated with MRS2211, a blocker of ADP receptor P2Y13. RT-PCR and  Western blot analysis con&#64257;rmed the presence of P2Y13. Cells exhibited signi&#64257;cant ectoATPase activity, which according to RT-PCR  analysis can be assigned to ENTPDase2. Both carbenoxolone, a  blocker of conductive ATP release, and brefeldin A, an inhibitor of  exocytosis, were able to partially decrease ATPe accumulation, pointing to the presence of at least two mechanisms for ATP release. Thus,  in Huh-7 cells, hypotonic treatment triggered the release of ATP.  Conversion of ATPe to ADPe by ENTPDase 2 activity facilitates the  accumulated ADPe to activate P2Y13 receptors, which mediate complete RVD.","palabras_clave":"CELL VOLUME|HUMAN HEPATOMA|NUCLEOTIDES|P2Y13 RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63559,"idioma_id":4,"titulo":"Digital Image Analysis of Distribution of Immiscible Fluids in Natural Porous Media","resumen":"This article proposes a method of digital image analysis to monitor immiscible displacement in soil samples. Four different natural soil samples were studied: coarse, medium, fine and silty sand. Paraffin oil and a sodium fluorescein solution were used as the displaced and displacing phase, respectively. Immiscible displacement tests were performed in a plexiglass cell and monitored by means of digital image analysis and direct volumetric measurement. The images were processed to obtain gray levels at different stages during the tests. Saturation of paraffin oil was then computed from the gray level and a linear regression equation was developed for each soil tested. The proposed method enabled valuable information to be obtained of the processes  that take place at the face of the samples during displacement and in the middle of the samples at the end of the tests. This procedure was very effective for analyzing different flow patterns, isolating areas with trapped paraffin oil, and to determining the ganglia size distribution at  different stages of the immiscible displacement tests. The results indicated that particle size and microstructure heterogeneities determine the flow pattern features observed during the immiscible displacement of paraffin oil by water.","palabras_clave":"IMMISCIBLE DISPLACEMENT|LNAPL|DIGITAL IMAGE ANALISYS|FLOW"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63560,"idioma_id":4,"titulo":"Synthesis and antifungal activity of C-21 steroids with an aromatic D ring","resumen":"Six analogues of salpichrolides with a simpli&#64257;ed side chain (6\u201311) were synthesized using a new methodology to obtain steroids with an aromatic D-ring. The key step was the elimination of HBr in a vicinal  dibromo D-homosteroid by treatment with 1,4-diazabicyclo[2.2.2]octane (DABCO). All new compounds  were completely characterized by 2D NMR techniques and tested on two fungal pathogenic species,  Fusarium virguliforme and Fusarium solani.","palabras_clave":"Anillo D aromático | Salpichrolidos |DABCO |Actividad antifúngica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63561,"idioma_id":4,"titulo":"Osmotic dehydration of apple: Influence of sugar and water activity on tissue structure, rheological properties and water mobility","resumen":"Osmotic dehydration of apple tissue (Malus pumila, Granny Smith cultivar) to water activity (aw) 0.97 or  0.94 with maltose or maltose syrup solutions was studied and compared with previous results using glucose  or trehalose as humectants. Structure (optical and transmission electronic microscopy observations),  rheological properties (small scale dynamic oscillatory and creep\/recovery measurements and large scale  compression force-deformation testing), and water mobility (1H NMR spectra) of parenchymatous apple  tissue were significantly affected by osmotic treatments. Osmotically dehydrated apples became soft and  extensible and lost crispness and hardness, while the behavior of the moduli G0 and G00 indicated weaker  gels after osmosis. Compression properties of the tissues abruptly changed after osmotic dehydration to  aw 0.97, while reduction to aw 0.94 led to a compression response more similar to that of untreated  apples. Compression behavior and state and distribution of water in apple tissues were influenced by  the osmotic agent employed and the aw level, while in general mechanical spectra and creep analysis  were not able for distinguishing physical differences between osmotic treatments assayed.","palabras_clave":"Apple|osmosis|Water mobility|sugars|rheology|structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63562,"idioma_id":4,"titulo":"An initial meteoroid stream survey in the southern hemisphere using the Southern Argentina Agile Meteor Radar (SAAMER)","resumen":"We present in this manuscript a 4 year  survey of meteor shower radiants utilizing the Southern Argentina Agile Meteor  Radar (SAAMER). SAAMER, which operates at the southern most region of South  America, is a new generation SKiYMET system designed with significant  differences from typical meteor radars including high transmitted power and an  8-antenna transmitting array enabling large detected rates at low zenith  angles. We applied the statistical methodology developed by Jones and Jones  (Jones, J., Jones, W. [2006]. Month. Not. R. Astron. Soc. 367, 1050\u20131056) to  the data collected each day and compiled the results into 1 composite  representative year at 1_ resolution in Solar Longitude. We then search for enhancements  in the activity which last for at least 3 days and evolve temporally as is  expected from a meteor shower. Using this methodology, we have identified in  our data 32 shower radiants, two of which were not part of the IAU commission  22 meteor shower working list. Recently, SAAMER\u2019s capabilities were enhanced by  adding two remote stations to receive meteor forward scatter signals from  meteor trails and thus enable the determination of meteoroid orbital  parameters. SAAMER started recording orbits in January 2012 and future surveys  will focus on the search for unknown meteor streams, in particularin the  southern ecliptic sky.","palabras_clave":"meteoroids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63563,"idioma_id":4,"titulo":"Dairy Herd Mastitis Program in Argentina: Farm Clusters and Effects on Bulk Milk Somatic Cell Counts","resumen":"        This research has been conducted to characterize dairy farm clusters  according to  mastitis control program practiced among small and medium dairy producer from  Argentina, and also to evaluate the effect of such farm cluster patterns on  bulk milk  somatic cell count (BMSCC). Two samples of 51 (cross-sectional) and 38  (longitudinal) herds were selected to identify farm clusters and study the  influence  of management on monthly BMSCC, respectively. The cross-sectional sample  involved the milking routine and facilities assessment of each herd visited.  Hierarchical cluster analysis was used to find the most discriminating farm  attributes in the cross sectional sample. Afterward, the herd cluster  typologies were  identified in the longitudinal sample. Herd monthly BMSCC average was evaluated  during 12 months fitting a linear mixed model. Two clusters were identified,  the  farms in the Cluster I applied a comprehensive mastitis program in opposite to  Cluster II. Post-dipping, dry cow therapy and milking machine test were  routinely  applied in Cluster I. In the longitudinal study, 14 out of 38 dairy herds were  labeled  as Cluster I and the rest were assigned to Cluster II. Significant difference  in  BMSCC was found between cluster I and II (60,000 cells\/mL). The present study  showed the relevance and potential impact of promoting mastitis control  practices  among small and medium sized dairy producers in Argentina.        &nbsp;  ","palabras_clave":"CLUSTER ANALYSIS |DRY COW THERAPY |MASTITIS CONTROL |MASTITIS MANAGEMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63564,"idioma_id":4,"titulo":"Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus)","resumen":"The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.","palabras_clave":"epididymis|sperm morphology|reproduction cycle|viscacha"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63565,"idioma_id":4,"titulo":"Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH).","resumen":"Knobs heterochromatic blocks occur in all Zea species with 2n = 20, varying in size and number across maize races and their wild relatives (Kato, Mass. Agric. Exp. Stn. Bull.635: 1-185, 1976; McClintock et al., Colegio de Postgraduados, Chapingo, México, 1981; González & Poggio, Genome 54: 26\u201332, 2011; Poggio et al., Cytogenet. Genome Res. 109: 259\u2013267, 2005). Variation in DNA content has been proposed to be principally due to differences in the amount of heterochromatin, which is mainly located in distal knobs(Laurie & Bennett, Herediy 55:307-313, 1985; Poggio et al., Ann. Bot. 82:115-117, 1998).  These structures can be observed as subtelomeric bands by DAPI-banding, and using Fluorescent In Situ Hybridization (FISH) (González et al., Chrom. Res. 14: 629-635, 2006).  Knobs have been described as highly repeated tandem arrays of 180-bp and TR-1 (350 bp)sequences, both repeated in different proportions constituting different knobs (Dennis & Peacock, J. Mol. Evol. 20: 341-350, 1984; Ananiev et al., Proc. Natl. Acad. Sci. 95: 10785-10790, 1998).  In this study we present the karyotypic formulae, asymmetry indexes and the position and composition of the knobs of two races of maize from Argentine Northeast(NEA), Tupi Amarillo (VAV 6563) and Rosado (VAV 6565). DAPI-banding and FISH techniques were applied. The plant material has been provided by the Vavilov Lab. of Universidad de Buenos Aires (UBA), and cultivated in the greenhouse of the Facultad deAgronomía, UBA.","palabras_clave":"MAIZE RACES|KARYOTYPICAL STUDIES|NORTHEAST ARGENTINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63566,"idioma_id":5,"titulo":"Knobs number variability in Argentine Andean maize populations (races Amarillo Grande y Garrapata)","resumen":"In this work we studied six populations belonging to two races of maize native of northwestern Argentina (NOA) in order to analyze the variation respect to the number of heterochromatic blocks (knobs), using fluorescent banding techniques (DAPI banding).    Cámara Hernández et al., 2011 (Razas de maíz nativas de la Argentina. Ed. Facultad de Agronomía, 168pp.) identified and described 28 native maize races from NOA. Two of these races, Amarillo Grande and Garrapata, differ by their morphological maize ears and grains, and   grow at different altitudes in the provinces of Jujuy and Salta (Argentina).   Zea taxa with 2n = 20 chromosomes are variable in number, size and position of the knobs, which can be found in 34 different chromosomal positions in corn and teosinte (Kato, Mass. Agr. St. Re. Exp. B. 635:1-185, 1976; McClintock, Maize Breeding and Genetics 59-184,   1978). This variability was used for the characterization of maize races of Latin America(Grobman et al., 1961. NAS-NRC, 375 pp.; Longley, J. Agron. Res 56:177-195, 1938; Ramírez et al., 1960. NAS-NRC, 159pp; Wellhausen et al., 1957. NAS-NRC, 138 pp.). Number and size variations of the knobs are related to intra and interspecific differences in DNA content found in the genus Zea (Tito et al., Theor. Appl. Genet. 83:58-64, 1991; Rosato et al., Am J. Bot. 85:168-174, 1998, Poggio et al., Ann. Bot. 82:115-117, 1998, Laurie & Bennett, Heredity 55:307-313, 1985).   In this work, we studied the number of knobs, using DAPI chromosome banding Summer, 1990. Chromosome banding. Ed. Unwin Hyman, 434 pp.) on interphase nuclei and mitotic metaphases.","palabras_clave":"MAIZE|CYTOGENETICS|KNOBS|DAPI BANDING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63567,"idioma_id":4,"titulo":"On the connection between complementarity and uncertainty principles in the Mach-Zehnder interferometric setting","resumen":"We revisit the connection between the complementarity and uncertainty principles of quantum mechanics within the framework of Mach\u2013Zehnder interferometry. We focus our attention on the trade-off relation between complementary path information and fringe visibility. This relation is equivalent to the uncertainty relation of Schrödinger and Robertson for a suitably chosen pair of observables. We show that it is equivalent as well to the uncertainty inequality provided by Landau and Pollak. We also study the relationship of this trade-off relation with a family of entropic uncertainty relations based on R´enyi entropies. There is no equivalence in this case, but the different values of the entropic parameter do define regimes that provides us with a tool to discriminate between non-trivial states of minimum uncertainty. The existence of such regimes agrees with previous results of Luis (2011 Phys. Rev. A 84 034101), although their meaning was not sufficiently clear. We discuss the origin of these regimes with the intention of gaining a deeper understanding of entropic measures.","palabras_clave":"UNCERTAINTY PRINCIPLE|COMPLEMENTARITY|MACH-ZEHNDER INTERFEROMETER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63568,"idioma_id":4,"titulo":"Detection of antibodies against Brucella abortus, Leptospira spp., and Apicomplexa protozoa in water buffaloes in the Northeast of Argentina","resumen":"Water buffalo industry has become a profitable activity worldwide, including the Northeast of Argentina (NEA). However, research on diseases affecting this species is scarce. The aim of the present study was to detect antibodies against Brucella abortus, Leptospira spp., Neospora caninum, Toxoplasma  gondii and Sarcocystis spp., in 500 water buffalo cows from five ranches (100 animals each) in the NEA. Serum samples were tested for B. abortus by fluorescence polarization assay (FPA), Leptospira spp. by  micro agglutination test (MAT), and N. caninum, T. gondii and Sarcocystis spp. by indirect fluorescen antibody tests (IFAT). Overall, the proportion of seropositive animals was 6.4, 22.2, 42.2, 25.4 and50.8% for brucellosis, leptospirosis, neosporosis, toxoplasmosis and sarcocystosis, respectively. The proportion of seropositive animals for all diseases was statistically different among herds (p<0.05). Statistically differences were also detected among age groups for brucellosis and neosporosis (p<0.05).The detection of specific antibodies to B. abortus, Leptospira spp. and several Apicomplexa protozoans in water buffaloes in the NEA is reported in this study.","palabras_clave":"Bubalus bubalis|Brucella abortus,|Leptospira spp.|Apicomplexa protozoa."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63569,"idioma_id":5,"titulo":"Patrones biogeográficos de los helechos de las sierras de Córdoba (Argentina) y sus implicancias en la conservación.","resumen":"Los atlas biogeográficos son la síntesis de patrones de distribución representada por trazos individuales, generalizados, nodos, áreas de endemismo y cladogramas de áreas, y a partir de ellos es posible establecer áreas prioritarias a conservar. Los helechos son indicadores de calidad de los hábitats y su falta de relaciones coevolutivas con vectores bióticos, así como su monofilia y estabilidad morfológica los hacen apropiados para establecer patrones biogeográficos. Nuestro objetivo fue aplicar análisis de trazos y parsimonia de endemismos a la flora de helechos de las Sierras de Córdoba (78 taxones) para contribuir a la elaboración del Atlas Biogeográfico centro-argentino. El patrón distribucional encontrado muestra las estrechas relaciones de estos helechos con las floras de las Sierras Subandinas; los Andes del Norte, los sistemas serranos de Buenos Aires y el sur de Brasil, correspondiéndose con el Arco Peripampásico Serrano. La protección de un espacio geográfico rico y complejo, con taxones endémicos y de distribución más amplia, garantiza preservar las condiciones en las que los taxones se desenvuelven. En este sentido, las sierras de Córdoba son un área prioritaria para conservar, porque constituyen un nodo o área biogeográficamente compleja, con una riqueza apreciable en especies de helechos y orígenes históricos, siendo un centro de convergencia biótica.","palabras_clave":"Helechos|Distribución|Chaco serrano|Panbiogeografía|Parsimonia de endemismos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63570,"idioma_id":5,"titulo":"Diversidad de helechos y licofitas del Parque Nacional Calilegua, Provincia de Jujuy, Argentina","resumen":"La provincia biogeográfica de las Yungas es considerada por la UNESCO como una de las  Reservas de la Biosfera. El Parque Nacional Calilegua es uno de los ?core? de estas Yungas, donde los  helechos y licofitas crecen en diferentes ambientes como epifitos, terrestres o saxicolas. El objetivo de  este trabajo es dar a conocer los helechos y licofitas que habitan en el Parque Nacional Calilegua, con  el fin de contribuir a la información sobre la biodiversidad en las Yungas y aportar a su conservación.  El estudio se realizó teniendo en cuenta el material colectado en viajes de campo por diferentes zonas  del Parque, durante los meses de diciembre a mayo de los años 2007 a 2011. Asimismo, se consultaron  distintos herbarios nacionales y bibliografía específica. Se registraron en el PN Calilegua un total de 103  taxones (especies y variedades) de helechos y licofitas, agrupados en 18 familias y 43 géneros. Se dan  a conocer seis nuevas citas para la provincia de Jujuy y se presentan actualizaciones nomenclaturales y  claves para la identificación de los taxa registrados.","palabras_clave":"Yungas|Biodiversidad|Helechos|Licofitas|Parque Nacional Calilegua"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63571,"idioma_id":4,"titulo":"Effect of a reservoir in the water quality of the Reconquista river, Buenos Aires, Argentina","resumen":"Abstract The lower portion of the Reconquista River is highly polluted. However, little is known about the state of the high and middle basins. The aims of this work were to assess the water quality on the high and  middle Reconquista River basins and to determinate if the presence of a reservoir in the river has a positive effect on the water quality. We conducted a seasonal study between August 2009 and November 2010 at  the mouth of La Choza, Durazno, and La Horqueta streams at the Roggero reservoir?which receives the water from the former streams?at the origin of the Reconquista River and 17 km downstream from the reservoir. We measured 25 physical and chemical parameters, including six heavy metal concentrations, and performed a multivariate statistical analysis to  summarize the information and allow the interpretation of the whole data set. We found that the Durazno and La Horqueta streams had better water quality than La Choza, and the presence of the reservoir contributed  to the improvement of the water quality, allowing oxygenation of the water body and processing of organic matter and ammonia. The water quality of the Reconquista River at its origin is good and similar  to the reservoir, but a few kilometers downstream, the water quality declines as a consequence of the presence of industries and human settlements. Therefore,the Roggero reservoir produces a significant improvement of water quality of the river, but the discharge of  contaminants downstream quickly reverses this effect.","palabras_clave":"RECONQUISTA RIVER BASIN|WATER QUALITY|DEPURATION|RESERVOIR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63572,"idioma_id":4,"titulo":"Periphyton responses to non-point pollution in naturally eutrophic conditions in Pampean streams","resumen":"The aim of this study was to know the effects of different intensities of non-point pollution on the water quality and the periphyton community in natural eutrophic environments as the Pampean streams. Non-point pollution produced by agricultural and farm activities are an important contributor to water quality deterioration. Our hypothesis was that, in spite of the natural water eutrophication of the pampean streams, the pollution generated by rural activities promote changes in water quality modifying nutrient concentrations and promoting or inhibiting the periphyton developed depending of kind and intensity of land use. The more important periphyton parameters to assess the non-point pollution effect due to rural activities estimated were chlorophyll-a, net production and alkaline phosphatase activity. The water quality parameters useful to differentiate rural activities were soluble reactive phosphorus and conductivity.  Agriculture caused an increase of authotrophic biomass and then increases net production whereas cattle farming caused an increase of heterotrophic biomass. The chlorophytes were abundant in agricultural zones whereas cyanophytes or euglenophytes were more abundant in the cattle farming areas. Thus, periphyton could be used as indicator of non-point pollution, differentiating not only the types kinds of activit but also their  persistence and the  intensity of rural practices.","palabras_clave":"PERIPHYTON|NON-POINT POLLUTION|PAMPEAN STREAMS|CATTLE FARMING|AGRICULTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63573,"idioma_id":5,"titulo":"Origen e Impacto de dos líneas de trabajo en extensión en Ciencias Agropecuarias","resumen":"El objetivo de este trabajo es presentar una experiencia de diez años (2002-2011) en desarrollar dos líneas de trabajo en extensión universitaria en servicios, transferencia y asesoramiento, particularmente en lo referido al origen, las dificultades presentadas y a los impactos producidos en los diferentes actores sociales destinatarios. Se tomaron como casos de estudio dos líneas en extensión de la Cátedra de Microbiología Agrícola de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Córdoba  ('Difusión del uso de inoculantes', y 'Mejoramiento de la calidad del agua') originados a partir de demandas de servicios. Las demandas provenían de las actividades de docencia, debido al ámbito socio\/familiar de los alumnos (establecimientos rurales y municipios) y estaban relacionadas con áreas de vacancia (calidad microbiológica de recursos e insumos para uso agropecuario). A lo largo de los diez años analizados, las demandas provinieron no sólo de productores agropecuarios, sino también de industrias productoras de inoculantes, industrias interesadas en tratamientos de agua y efluentes, instituciones públicas, medios de prensa y ONGs. La cantidad y tipo de actividades requeridas variaron sustancialmente entre los años, dependiendo de las ofertas de servicios privados y de las condiciones económicas de los sectores involucrados. Se presenta un análisis de: a) los criterios que se debieron considerar para establecer normas de funcionamiento: alta eficiencia, objetividad e imparcialidad y transparencia en el manejo de los fondos generados por el servicio; b) la relación con los destinatarios solicitantes: particularmente empresarios y medios de prensa, y c) el impacto de los servicios en la unidad académica y la sociedad. Se concluye que las líneas de trabajo en extensión originadas en demandas de servicios tienen un fuerte impacto en la sociedad debido a que permanentemente se retroalimentan con requerimientos concretos. Por tales motivos, deben tener alta flexibilidad en el funcionamiento para adaptarse a los cambios socio\/productivos de los actores involucrados.","palabras_clave":"DEMANDA DE SERVICIOS|PRODUCTORES AGROPECUARIOS|ASESORAMIENTO A EMPRESAS|PLANTA PILOTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63574,"idioma_id":5,"titulo":"LA MATRIZ DE VIABILIDAD EMPRESARIA RSE Aplicación al caso de las empresas mineras de Tandil ante cambios de contexto","resumen":"El artículo vincula la matriz de viabilidad empresaria RSE, producto del trabajo del equipo de investigación, con las circunstancias que dieron forma al llamado conflicto canteril en la ciudad de Tandil, desde 2007 hasta 2011. En el trabajo se clasifican los acontecimientos según significan diversas posiciones de las empresas canteriles, y diversos cambios en el entorno a través de actores como la Asamblea Ciudadana por la Preservación de las Sierras, los agentes turísticos y los actores institucionales y políticos. A raíz de cambios en el entorno, la viabilidad RSE de las  empresas canteriles se torna primero dificultosa, y luego imposible. Como resultado, el caso muestra la posibilidad concreta de aplicar la matriz para construir una mejor relación de la empresa con la comunidad, cuando los valores de la comunidad se van volviendo más exigentes en terminos de RSE.","palabras_clave":"MATRIZ DE VIABILIDAD RSE|RSE Y MEDIO AMBIENTE|RSE Y PYME|ENTORNO-EMPRESA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63575,"idioma_id":4,"titulo":"Graphene  paste  electrode:  Electrochemical behavior  and  analytical  applications for the quantification of NADH.","resumen":"This work reports a critical study of the electrochemical behavior of a graphene paste electrode (GrPE) obtained by mixing graphite, mineral oil, and graphene obtained by reduction of graphene oxide with ascorbic acid and characterized by IR and Raman spectroscopy. The GrPE was characterized by scanning electron microscopy, cyclic voltammetry and amperometry. The effect of the GrPE composition on the electrochemical response of the resulting electrodes was studied using different redox probes. GrPE selectively catalyzes the electrooxidation of different bioanalytes such as ascorbic acid, dopamine and NADH without suffering common surface passivation. The advantages of GrPE for the simultaneous, selective and sensitive quantification of NADH in the presence of ascorbic acid are also reported.","palabras_clave":"GRAPHENE|GRAPHENE PASTE ELECTRODE|COMPOSITE ELECTRODE|NADH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63576,"idioma_id":4,"titulo":"Trophic relationships between a Patagonian gastropod and its epibiotic anemone revealed by using stable isotopes and direct observations","resumen":"The carnivorous snail Adelomelon ancilla  usually carries, attached to its shell, the anemone Antholoba  achates, which also lives attached to hard substrates in  the same area. Interaction between both species was studied  by stable isotopes analyses (SIA), direct observations,  and analysis of gut contents. Results did not show evidences  of dietary overlap between anemones and snails.  A. ancilla consumed mainly bivalves and secondarily  gastropods. The diet of A. achates involved sea urchins and  echiurids as main prey. The trophic niches of anemones  and snails did not overlap; the species had similar d15N  values but differences in their d13C values. Sessile and  epibiotic anemones also showed differences in their d13C  means, revealing access to different resources. Whereas  SIA provided information on trophic relationships and  structure, observation of feeding events provided details of  prey utilization patterns. Combining direct and indirect  approaches helps to overtake method weaknesses and  greatly improve dietary descriptions.","palabras_clave":"DIET|ISOTOPIC-ANALYSYS|TROPHIC-INTERACTION|VOLUTID"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63577,"idioma_id":4,"titulo":"Inhibition of Fast Axonal Transport by Pathogenic SOD1 Involves Activation of p38 MAP Kinase","resumen":"Dying-back degeneration of motor neuron axons represents an established   feature of familial amyotrophic lateral sclerosis (FALS) associated with   superoxide dismutase 1 (SOD1) mutations, but axon-autonomous effects of   pathogenic SOD1 remained undefined. Characteristics of motor neurons   affected in FALS include abnormal kinase activation, aberrant   neurofilament phosphorylation, and fast axonal transport (FAT) deficits,   but functional relationships among these pathogenic events were   unclear. Experiments in isolated squid axoplasm reveal that FALS-related   SOD1 mutant polypeptides inhibit FAT through a mechanism involving a   p38 mitogen activated protein kinase pathway. Mutant SOD1 activated   neuronal p38 in mouse spinal cord, neuroblastoma cells and squid   axoplasm. Active p38 MAP kinase phosphorylated kinesin-1, and this   phosphorylation event inhibited kinesin-1. Finally, vesicle motility   assays revealed previously unrecognized, isoform-specific effects of p38   on FAT. Axon-autonomous activation of the p38 pathway represents a   novel gain of toxic function for FALS-linked SOD1 proteins consistent   with the dying-back pattern of neurodegeneration characteristic of ALS.","palabras_clave":"Superoxide dismutase 1|Fast axonal transport|p38 Map kinase|Kinesin 1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63578,"idioma_id":4,"titulo":"The Discovery of Stromatolites Developing at 3570 m above Sea Level in a High-Altitude Volcanic Lake Socompa, Argentinean Andes","resumen":"The water at the site of stromatolites formation is alkaline, hypersaline, rich in inorganic nutrients, very rich in arsenic, andwarm (20?24uC) due to a hydrothermal input. The stromatolites do not lithify, but form broad, rounded and low-domed bioherms dominated by diatom frustules and aragonite micro-crystals agglutinated by extracellular substances. In comparison to other modern stromatolites, they harbour an atypical microbial community characterized by highlyabundant representatives of Deinococcus-Thermus, Rhodobacteraceae, Desulfobacterales and Spirochaetes. Additionally, a high proportion of the sequences that could not be classified at phylum level showed less than 80% identity to the best hit in the NCBI database, suggesting the presence of novel distant lineages. The primary production in the stromatolites is generally high and likely dominated by Microcoleus sp. Through negative phototaxis, the location of these cyanobacteria inthe stromatolites is controlled by UV light, which greatly influences their photosynthetic activity. Diatoms, dominated by Amphora sp., are abundant in the anoxic, sulfidic and essentially dark parts of the stromatolites. Although their origin in the stromatolites is unclear, they are possibly an important source of anaerobically degraded organic matter that induces in situ aragonite precipitation. To the best of our knowledge, this is so far the highest altitude with documented actively forming stromatolites. Their generally rich, diverse and to a large extent novel microbial community likely harbours valuable genetic and proteomic reserves, and thus deserves active protection. Furthermore, since the stromatolites flourish in an environment characterized by a multitude of extremes, including high exposure to UV radiation, they can be an excellent model system for studying microbial adaptations under conditions that, at least in part, resemble those during the early phase of life evolution on Earth.","palabras_clave":"STROMATOLITES|MODERN LAKES|PUNA|ANDES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63579,"idioma_id":4,"titulo":"Differentiability and best local approximation","resumen":"In this paper we give sucient conditions over the diff\u000Berentiability  of a function to assure existence of the best local approximant in Lp-spaces,  0 < p  <1. These conditions are weaker than those given in previous papers.  For p = 2 we show that, in a certain way, they are also necessary. In addition,  we characterize the best local approximant.","palabras_clave":"BEST  APPROXIMATION|L^P-NORM|LOCAL APPROXIMANT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63580,"idioma_id":4,"titulo":"Gymnophalloides  nacellae  n.  sp.  (Digenea:  Gymnophallidae), amended diagnosis of the genus Gymnophalloides Fujita, 1925 and its inclusion in Parvatrematinae: morphological and molecular evidences","resumen":"Morphological and molecular evidence suggest that specimens formerly described as Lacunovermis sp. from Nacella (Patinigera) spp (Patellogastropoda: Patellidae) belong to a new species of Gymnophalloides Fujita, 1925. Based on the new information, they are identified as Gymnophalloides nacellae n. sp. The new species differs from Gymnophalloides tokiensis, Gymnophalloides seoi, and Gymnophalloides heardi mainly through the presence of a group of papillae located on the ventral surface between oral and ventral suckers. A detailed morphological study revealed the lack of pars prostatica, a character previously reported in G. seoi, which is why it was formerly placed in the Gymnophallinae. Molecular information proved that G. nacellae is close to G. seoi, being nestled together with Parvatrema representatives. This molecular information, along with the absence of pars prostatica,  allows these 2 genera to be placed in Parvatrematinae. An amended diagnosis of Gymnophalloides is provided. Histological sections of  mantle epithelium of the limpet show metacercariae attached by their oral and ventral suckers in a similar manner to G. seoi in its host, the oyster Crassostrea gigas. Tissue reaction includes cells of outer mantle epithelium being stretched by sucker attachment, hemocyte infiltration of connective tissue between mantle epitheliums, and abnormal calcareous deposition on the inner surface of the shell.","palabras_clave":"MORPHOLOGICAL EVIDENCE|MOLECULAR EVIDENCE|GYMNOPHALLOIDES NACELLAE|NACELLA (PATINIGERA) SPP."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63581,"idioma_id":4,"titulo":"Temperature response of luminescent tris(bipyridine)ruthenium(II)-doped silica nanoparticles","resumen":"Nanoparticle-based temperature imaging is an emerging field of advanced applications. Herein, the sensitivity of the phosphorescence of tris(bipyridine)ruthenium(II)-doped silica nanoparticles towards temperature is studied. 130 nm size particles were prepared by a modification of Stöber?s method, that allows the incorporation of Ru[(bpy)3]2+ into the outer particle shell. The entrapped Ru[(bpy)3]2+ retains its photophysical properties, yet the emission of the particles is not affected by the presence of O2, neither by anionic quenchers; quenching by MV2+, on the other hand, is strongly dependent on pH. Between 20 and 60 °C, the steady-state emission of the particles decreases linearly with increasing temperature. The slope of the straight line diminishes slightly on thermal cycling, but soon stabilizes. Fluorescence measurements by scanning confocal microscopy indicate that the silica nanoparticles doped with Ru[(bpy)3]2+ can indeed be employed to probe thermal processes in micro-environments.","palabras_clave":"fluorescent nanoparticles|fluorescence quenching |doped silica| tris(bipyridine)ruthenium(II)|luminescent temperature nanosensors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63582,"idioma_id":4,"titulo":"Controlled Pd deposition on fibers by electroless plating. The effects of the support on the reduction of nitrite in water","resumen":"tPd catalysts supported on activated silica?alumina fibers (CFs) and activated carbon felts (ACFs) wereprepared and evaluated for the reduction of nitrite in water in a batch reactor. The electroless plating (ELP)method was used in order to deposit small Pd particles on the surface of the fibers. While a homogeneousdistribution of Pd nanoparticles was obtained for carbon fibers, this was not the case for ceramic onesin which non-homogeneous patches of Pd films were formed. This fact resulted in a higher activity fornitrite reduction when carbon fibers were used. However, the selectivity toward the undesired productammonia, measured at the end of the reaction, was slightly higher for Pd\/ACFs catalysts.","palabras_clave":"NITRATES|NITRITES|REDUCTION|WATER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63583,"idioma_id":4,"titulo":"Influence of culture conditions on laccase production, growth and isoenzymes patterns in native white rot fungi from the Misiones rainforest (Argentina)","resumen":"Many biotechnological processes pursuing sustainability search effective, inexpensive and environmentally friendly alternatives to replace conventional practices. Lignocellulolytic white rot fungi can serve for this task through their enzymatic system including laccase (Lac). One of purposes of biotechnological enzymes production processes is usually to find the best growing and secretion conditions for a particularly selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633 and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33°C) and pHs (3.5, 4.5, 5.5) under static conditions for 7, 10 and 14 days to evaluate their growing ability and Lac production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U\/l)  at 29ºC and pH 4.5 at the 10th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature and time of cultivation for the different tested fungi.","palabras_clave":"White rot fungi|Laccase|Culture conditions|Isoenzymes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63584,"idioma_id":4,"titulo":"Detection of minimally disseminated disease in the cerebrospinal fluid of children with high-risk retinoblastoma by reverse transcriptase-polymerase chain reaction for GD2 synthase mRNA","resumen":"To evaluate minimally disseminated disease (MDD) in   cytologically negative cerebrospinal fluid (CSF) specimens of patients   with high-risk retinoblastoma by the detection of the synthase of ganglioside GD2 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). METHODS: The CSF was evaluated in 26 patients   with high risk for CSF relapse: 14 with postlaminar optic nerve invasion, five of them with tumour at the resection margin, five with massive choroidal invasion, three with overt orbital extension and four patients with systemic metastasis. Serial CSF examinations were repeated at different time intervals according to stage and in the event of suspected relapse. GD2 synthase mRNA was evaluated by RT and nested PCR at each procedure. RESULTS: MDD was present at diagnosis in six cases (23%) and it was significantly associated to massive optic   nerve involvement or history of glaucoma (p<0.05). Three of the children with positive MDD had a CSF relapse. Thirteen patients had negative MDD at diagnosis and one had a CSF relapse. In seven children no ARN could be obtained for PCR analysis and two subsequently relapsed. The probability of CSF relapse was 0.50 (95% confidence interval (CI) 0.13-0.88) for children with MDD and 0.08 (95% CI 0.02-0.46) for those with negative RT-PCR examination of the CSF at diagnosis (p=0.03). CONCLUSIONS:  MDD in the CSF detected by RT-PCR for GD2-synthase mRNA occurred in 31.7% of evaluable high-risk children with retinoblastoma with no initial central nervous system (CNS) involvement. It was significantly associated to optic nerve involvement and glaucoma and increased risk of CSF relapse.","palabras_clave":"MINIMALLY DISSEMINATED DISEASE |CEREBROSPINAL FLUID |RETINOBLASTOMA|GD2 SYNTHASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63585,"idioma_id":4,"titulo":"Characterization of glucose electro-oxidation at Ni and Ni-Cr alloy electrodes","resumen":"Electro catalytic oxidation of glucose by the nickel oxy-hydroxide film developed in  alkaline medium on different working electrodes materials (Ni, Ni-Cr (90:10) or Ni-Cr (80:20)) is  demonstrated. In the presence of glucose the oxidation peak current of nickel hydroxide increases  while that of the reverse process decreases. This is attributed to the mediation of nickel species in the  electro-catalytic process. The electro-catalytic film is formed by using a potential scan program  developed in our research group. This program facilitated the formation of stable proportions of  Ni(II)\/Ni(III) oxy-hydroxides which are necessary for the electro-catalysis study. The electrochemical  behavior of the electrodes in 0.10 M NaOH and in the presence of glucose was characterized by cyclic  voltammetry. The structural morphology and roughness of the oxy-hydroxides were characterized by  using, scanning electron microscopy and atomic force microscopy. The nickel electrode, as well as  nickel-chromium alloy electrodes, showed good catalytic activity for the anodic oxidation of glucose. A  new and more complete reaction mechanism based on the electrochemical generation of a Ni(III)  active site at the electrode surface and the formation of a complex between the analyte and this active  site prior to the electron transfer step is proposed. The Ni-Cr (80:20) alloy electrode gave the highest  oxidation peak current to glucose concentrations indicating a greater catalytic activity of the film,  attributed to an increased roughness of the electro-catalytic film.","palabras_clave":"NICKEL|NICKEL-CHROMIUM ALLOYS|ELECTROCATALYSIS|GLUCOSE|CYCLIC VOLTAMMETRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63586,"idioma_id":4,"titulo":"Catalysts of alkaline nitrates supported on oxides for the diesel soot combustion.  Deactivation by hydro-treatment and CO2","resumen":"&nbsp; The deactivation of supportedcatalysts CsNO3 and LiNO3 for diesel soot combustion is studied in this work. Theeffect of hydro-treatment at high temperature and presence of CO2 in feed flowwere investigated. Catalysts were characterized by means of X ray diffraction,DSC technique and FTIR. A fixed bed reactor was used to measure activity offresh and deactivated catalysts with NO2\/O2 feed. Thermogravimetrical systemwith air\/CO2 feed was used to study the deactivation with CO2. Catalysts withLiNO3 were the most resistant to deactivation by hydro-treatment, and they arenot deactivated by CO2. It is postulated that the cesium nitrate solubilizes inwater and is eliminated during the hydro-treatment, instead, lithium speciesinteracted more strongly with the support and generate more resistance species.&nbsp;","palabras_clave":"DIESEL SOOT|ALKALI NITRATES|DESACTIVATION|CATALYTIC COMBUSTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63587,"idioma_id":4,"titulo":"Physiological focus on the erythropoietin?hepcidin?ferroportin axis","resumen":"To analyze the interconnection between erythropoiesis and iron metabolism, one of the issues raised in this study is to know iron bioavailability under physiopathological conditions. Our aim was to understand the functional axis response composed of Erythropoietin (Epo)-Hepcidin-Ferroportin(FPN), when two dysfunctional states coexist, using an animal model of iron overload followed by hypoxia. FPN and Prohepcidin were assessed by immunohistochemistry using rabbit anti-mouse FPN polyclonal and Prohepcidin monoclonal antibodies. Goat-labeled polymer-HRP anti-rabbit Envision+System (DAB) was used as secondary antibody. Epo levels were measured by ELISA. Tissue iron was studied by Prussian Blue iron staining. Erythropoietic response was assessed using conventional hematological tests. Iron overload increased Prohepcidin that remained high in hypoxia, coexisting with high levels of Epo in hypoxia, with or without iron overload. In hypoxia, FPN was clearly evident in reticuloendothelial macrophages, more than in hypoxia with iron overload. Interestingly, duodenal FPN was clearly identified on basolateral membrane in hypoxia, with or without iron overload. Our data indicate that two signals could induce the cell-specific response: Iron signal: That induced Prohepcidin, which reduce reticuloendothelial FPN reducing iron availability; Hypoxia signal: That stimulated Epo, affecting iron absorption by stabilizing duodenal FPN, allowing iron supply to erythropoiesis, independently of stores size.","palabras_clave":"FERROPORTIN|PROHEPCIDIN|ERYTHROPOIETIN|IRON"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63588,"idioma_id":4,"titulo":"Preparation and characterisation of calcined Mg\/Al hydrotalcites impregnated with alkaline nitrate and their activities in the combustion of particulate matter","resumen":"The effect of incorporating alkaline nitrates in hydrotalcites for use in the combustion of particulate matter  from diesel emissions has been studied. The catalysts were characterised by X-ray diffraction (XRD), N2 adsorption,  scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), elemental analysis  (EA), atomic absorption spectrophotometry (AAS) temperature programmed reduction (TPR) and Fourier  transform infrared spectroscopy (FTIR). Activity measurements were carried out using a thermobalance in  air and using a fixed-bed reactor with a NO\/O2 flow. The observed activities decreased in the following  order: HTMgAlcCs > HTMgAlcK > HTMgAlcLi > HTMgAlc.","palabras_clave":"HYDROTALCITES|PARTICULATE MATTER|IRFT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63589,"idioma_id":4,"titulo":"Shrub biomass estimation in the semiarid Chaco forest: a contribution to the quantification of an underrated carbon stock","resumen":"Context: The quantification of biomass of woody plants isat the basis of calculations of forest biomass and carbonstocks. Although there are well-developed allometricmodels for trees, they do not apply well to shrubs, andshrub-specific allometric models are scarce. There is thereforea need for a standardized methodology to quantifybiomass and carbon stocks in open forests and woodlands.Aims: To develop species-specific biomass estimationmodels for common shrubs, as well as a multispecies shrubmodel, for the subtropical semiarid Chaco forest of centralArgentina.Methods: Eight shrub species (Acacia aroma, Acaciagilliesii, Aloysia gratissima, Capparis atamisquea, Celtisehrenbergiana, Larrea divaricata, Mimozyganthuscarinatus, and Moya spinosa) were selected, and, on average,30 individuals per species were harvested. Their totalindividual dry biomass was related with morphometric variablesusing regression analysis.Results: Crown area as well as crown-shaped variablesproved to be the variables with the best performance forboth species-specific and multispecies shrub models. Theseallometric variables are thus recommended for standardizedshrub biomass assessments.Conclusion: By accounting for the shrub component of thevegetation, our models provide a way to improve the quantificationof biomass and carbon in semiarid open forest andwoodlands.","palabras_clave":"ALLOMETRIC MODELS|BIOMASS QUANTIFICATION|CARBON INVENTORIES|CHACO|DIMENSIONAL RELATIONSHIPS|SHRUBS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63590,"idioma_id":4,"titulo":"Differential expression of endocannabinoid system in normal and preeclamptic placentas: Effects on nitric oxide synthesis","resumen":"Anandamide (AEA) is a lipid mediator that participates in the regulation of several reproductive functions. This study investigated the endocannabinoid system in normal (NP) and preeclamptic (PE) placentas, and analyzed the potential functional role of AEA in the regulation of nitric oxide synthesis. The protein expression and localization of NAPE-PLD, FAAH and CB1 receptor were analyzed in normal and preeclamptic pregnancies using immunoblotting and immunohistochemistry. NAPE-PLD expression was shown to be significantly higher (p < 0.05) in PE tissues than in NP. In contrast, a decrease in FAAH protein (p < 0.001) was detected in placentas collected from women with preeclampsia. Both enzymes were mainly located in the syncytiotrophoblasts from normal and preeclamptic tissues. No differences were seen in CB1 receptor from both groups of placental villous. Exogenous and endogenous AEA significantly increased NOS activity. Although pre-incubation with AM251 (CB1 antagonist) had no effect, co-incubation with both AEA and AM251 diminished NOS activity from normal term placentas. We observed increased NOS activity in placental villous from women with preeclampsia compared with normotensive pregnant women. Furthermore, NOS activity from preeclamptic tissues was diminished by co-treatment with AM251, illustrating that the NO levels could be modulated by AEA. These data suggest that AEA may be one of the factors involved in the regulation of NOS activity in normal and preeclamptic placental villous. Interestingly, the differential expression of NAPE-PLD and FAAH suggests that AEA could play an important role in the pathophysiology of PE.","palabras_clave":"Placenta|Preeclampsia|Nitric Oxide|Endocannabinoids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63591,"idioma_id":4,"titulo":"Redistribution of high energy alpha particles due to sawteeth with partial reconnection","resumen":"The redistribution of high energy alpha particles due to internal kink modes is studied in plasmas with ITER-like parameters. The exact particle trajectories in the total fields, equilibrium plus perturbation, are calculated. Theequilibrium magnetic field is obtained by analytically solving the Grad?Shafranov equation and the perturbed electricand magnetic fields are reconstructed using ideal MHD and the experimental information about the displacementeigenfunction. The (1, 1), (2, 2) and (2, 1) modes are included and the effect of changing their amplitude andfrequency is determined. The results show that if the conditions are similar to those reported in Igochine et al (2007Nucl. Fusion 47 23), the peak density of counter-passing particles decreases between 25% and 40% (depending onthe energy); the peak of the trapped particles density shifts outwards by approximately 10% of the minor radius andthe total on axis density decreases by more than 25%. This redistribution occurs inside the q = 1 surface. Theaddition of a (2, 1) mode, which can produce the stochastization of the magnetic field, significantly increases particleredistribution and allows particles to spread beyond the q = 1 surface. Different groups of particles (co-passing,counter-passing, trapped) respond differently to the perturbations.","palabras_clave":"ITER|SAWTOOTH|PARTICLES|REDISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63592,"idioma_id":4,"titulo":"PROPERTIES OF POLYETHERSULPHONE ULTRAFILTRATION MEMBRANES MODIFIED WITH POLYETHYLENE GLYCOLS","resumen":"Polyethersulfone ultrafiltration membranes have been prepared by using PEGs, of: 400; 1,000 and 10,000 g\/mol, as additive with dimethylacetamide as solvent. IR analysis proves that PEG leaves almost completely the surface of the membranes after 24 hours of water immersion. SEM, contact angle, and liquid-liquid displacement porometry have been used to characterize the membrane morphology, surface hydrophilicity and porous structure. The relative flux reduction factor, flux, retention - of PEG (20,000 and 35,000 g\/mol) and BSA (67,000 g\/mol) - and pure water permeability have been measured for the membranes.Results show that the addition of PEG increases slightly hydrophilicity and decreases pore size and narrows the corresponding pore size distribution while thickening the skin layer, in spite of the fast disappearance of the added PEG form the membrane surface. The resulting flux and pure water permeability are higher when middle size PEGs are added but decrease again when very high molecular weight PEGs are added. Retention decreases initially for increasing molecular weights of PEG although for very long PEG chains (MW of 10,000 g\/mol) retention increases again. After filtration, the membranes with PEG added showed a lower relative flux reduction that decreases for increasing molecular weight of the added PEGs.","palabras_clave":"POLYETHERSULFONE ULTRAFILTRATION MEMBRANE|POLYETHYLENE GLYCOL ADDITIVE|MEMBRANE CHARACTERIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63593,"idioma_id":4,"titulo":"Maternal effects on the fecundity and egg quality of the Patagonian stock of the Argentine Hake (Merluccius hubbsi)","resumen":"The influence of age, size and condition of spawning females on fecundity and oocyte quality were analyzed for the Patagonian stock of Merluccius hubbsi. Samples of mature females were collected in the spawning area during two research surveys in January 2010 and 2011, during the peak of the reproductive season. Batch fecundity (BF) ranged between 40,500 (29 cm total length [TL]) and 2,550,000 (95 cm TL) hydrated oocytes, and was positively correlated with total length, gutted weight, age, hepatosomatic index (HSI) and the relative condition factor (Kn). Relative fecundity ranged between 85 and 1040 hydrated oocytes g-1 and showed significant positive relationships with gutted weight, HSI and Kn; however coefficients of determination were low for all regressions. Dry weights of samples of 100 hydrated oocytes ranged between 1.8 and 3.95 mg and were positively correlated with all variables analyzed, including batch and relative fecundity. Multiple regression models using the morpho-physiological characteristics of females supported maternal influences on fecundity and egg weights. Larger individuals had better somatic and egg condition, mainly revealed by higher HSI and hydrated oocytes with larger oil droplets. These results would be associated with the fact that larger females showed higher feeding activity during the spawning season in comparison with young individuals.","palabras_clave":"MATERNAL CONDITION|BATCH FECUNDITY|OOCYTE QUALITY|ARGENTINE HAKE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63594,"idioma_id":4,"titulo":"Impacts of composite wastewater on a Pampean stream (Argentina) and phytoremediation alternative with Spirodela intermedia Koch (Lemnaceae) growing in batch reactors","resumen":"Poultry slaughterhouses are producing increasing amounts of solid organic by-products and waste in Argentina. The objectives of this study are to assess the impacts of wastewater, mainly composed of poultry slaughterhouse effluent, on a Pampean stream and to evaluate the convenience of phytoremediation alternative with Spirodela intermedia. Impacts over La Choza stream (Buenos Aires, Argentina) were evaluated by means of physical and chemical characterization of the water upstream and downstream input. Nutrients and total suspended solids increased downstream of the open channel outlet. A bioassay was performed in order to evaluate the role of S. intermedia in wastewater treatment. Two treatments were performed in batch reactors during 144 h (6 days), with (treatment A) and without (treatment B) the addition of S. intermedia. Water samples were taken at 8, 24, 48, 96 and 144 h after the start of the assay for the determination of ammonium, nitrite, nitrate, soluble reactive phosphorus and total phosphorus. Since removal rates of ammonium and total phosphorus were significantly higher in treatment A than in treatment B and the nitrification process was highly increased in treatment A, then the species could be used for the phytoremediation of composite wastewater.","palabras_clave":"Wastewater|Nutrients|Spirodela intermedia|Batch reactor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63595,"idioma_id":4,"titulo":"Caiman gasparinae sp. nov., a huge alligatorid (Caimaninae) from the late Miocene of Paraná, Argentina","resumen":"          Morphological  studies of South American alligatorids are scarce and the phylogenetic  relationships within Caimaninae are poorly understood. In this context, a systematic revision and detailed morphological  description of the enigmatic Argentinean Miocene  caimanines are highly relevant. The current record is characterized by numerous species within the genus Caiman, although, morphological disparity  remains unclear. Here we describe Caimangasparinae sp. nov., which is one of  the largest known Caiman species from the late Miocene of Argentina. Similar to other Caimanspecies and Melanosuchus,  the new taxon has orbits larger than the infratemporal fenestrae and  small supratemporal fenestrae that are not lost, as in Paleosuchus.  Together with the absence of conspicuous rostral crests, the  shape of the snout distinguishes C. gasparinae from Melanosuchus.Caiman gasparinae can be further distinguishedusing other cranial  characters, such as a broad and low rostrum with a narial opening oriented dorsally, a relatively long interpremaxillary suture, and the occipital surface of the braincase with a poorly  developed crista tuberalis exposing the carotid foramina in lateral view.          &nbsp;  ","palabras_clave":"Alligatoridae|Caiman|Neogene|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63596,"idioma_id":4,"titulo":"Investigation of the Stability of Novozym® 435 in the Production of Biodiesel","resumen":"The effect of the composition of the feedstock (free fatty acids and methanol), the presence or absence of moisture and the contact with biodiesel on the catalytic, enzymatic and physical stability of Novozym® 435 during enzymatic biodiesel production was studied. The continuous removal of moisture leads to the deactivation of the biocatalyst and favours the degradation of the polymethylmethacrylate (PMMA) that constitutes the support of the Candida antarctica B lipase (CALB). Nevertheless, the presence of PMMA along with the active protein was detected in the reaction media regardless of the operating conditions. Surprisingly, biodiesel (without the presence of methanol) is able to diffuse inside the biocatalyst beads producing its swelling, modifying the internal texture and also dissolving the polymeric matrix.","palabras_clave":"NOVOZYM435|BIODIESEL|FAESTER|ENZYMATIC ESTERIFICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63597,"idioma_id":4,"titulo":"Backscattered Electron Images, X-ray Maps and Monte Carlo simulations applied to the study of plagioclase composition in volcanic rocks","resumen":"Zoning patterns in plagioclases are related to abrupt changes in the anorthite content along the crystal growing direction. Accurate characterization of these patterns by electron microprobe is useful to identify magma chamber processes such as recharge, mingling and whole-chamber overturn events. In this work, a new procedure to obtain high resolution quantitative maps of anorthite concentration in single plagioclase crystals is developed. The methodology consists of performing a calibration of backscattered electron images using quantitative X-ray maps. The ultimate resolution of characteristic X-rays and backscattered electron signals is studied by Monte Carlo simulation. The method is applied to characterize the chemical composition of a volcanic plagioclase from the Cerro Vilama, Argentina. The results obtained are more precise than the values given by the methods commonly used in the study of plagioclase composition, i.e. the classical profiling by electron microprobe point analysis or the modern backscattered electron image calibration by means of quantitative energy or wavelength dispersive X-ray analysis at a few selected points.","palabras_clave":"Backscattered electron imaging|X-ray maps|Plagioclase zoning"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63598,"idioma_id":4,"titulo":"Ectomycorrhizal fungal communities associated to Nothofagus species in Northern Patagonia","resumen":"Ectomycorrhizal fungi constitute an important  component of soil biota in Nothofagus forests in Patagonia.  However, ectomycorrhizal fungal community is poorly  known in this region. Here, we assess biodiversity and community  compositions of ectomycorrhizal fungal species associated  with Nothofagus dombeyi, N. obliqua and N. alpina.  We selected three monospecific Nothofagus forest sites for  each species within the boundaries of the Lanin National Park  in Northern Patagonia. Ectomycorrhizal fungal species were  identified based on morphotyping and rDNA (ITS and 28S  rDNA) sequence analysis using both universal and taxonspecific  primers. Contrary to previous studies on congeneric  host trees, our results showed no significant differences  among Nothofagus forest types in terms of fungal biodiversity  and community composition. However, altitude had a strong  effect on the structure of the ectomycorrhizal fungal community  associated with Nothofagus spp.","palabras_clave":"ECM fungi|Richness|Community composition|Nothofagus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63599,"idioma_id":4,"titulo":"When allowed, females prefer novel males in the polygynous subterranean rodent Ctenomys talarum (tuco-tuco).","resumen":"                      The ability to recognize familiar conspecifics  plays an important role at the time of choosing a mating partner in rodents. A  laboratory study using preference test was used in order to test the hypothesis  that, in the polygynous subterranean rodent C.  talarum, females prefer novel males when offered two odors, or individuals  (one familiar and one novel) limited in their movements so that male aggression  is prevented. Our findings show that females prefer novel tuco-tucos  at three levels of male assessment: odor samples (consisting of shavings  soiled with urine, faeces, and presumably, other body secretions collected from  the male home cage), confined males behind a wire mesh, and full  contact with tethered males. Previous studies of this species demonstrated that  in the wild, male-male competition and male coercion severely limit the  possibility of females mating non-neighbors, i.e. novel males. Females mating  neighbors to whom they are familiarized, obtain high quality mating since they  are territorial, highly competitive males. Nonetheless, when females have the  opportunity, as shown in the two-choice experiments, they choose novel males,  probably benefiting their progeny from novel genetic combinations. Hence,  combining evidence from laboratory and field studies in C. talarum, it is possible to better understand female preferences in  modelling individual reproductive strategies.  ","palabras_clave":"Ctenomys talarum|tuco- tucos|famialiarity|female choice|odors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63600,"idioma_id":4,"titulo":"High performance purification process of methyl and ethyl esters produced by transesterification","resumen":"The aim of this work is the obtention of high quality methyl and ethyl esters, particularly in those cases in which conventional purification procedures fail such as in the production of ethylesters or in the case of methylesters obtained from high acidity raw materials, e.g. in the range 1\u20134%. Three different methodologies were employed, according to the soap content of the crude biodiesel: (a) one washing step with a CO2 saturated solution; (b) two consecutive washing steps with CO2 saturated solutions; (c) first neutral washing using a small volume of water and a subsequent washing with a CO2 saturated solution. These procedures were compared with the classical method that uses a first acid washing followed by a neutral one. The washings with CO2 saturated solutions showed excellent performance, giving a final product with quality parameters similar to the ones obtained with conventional methods (washing with HCl solution). It was demonstrated that the utilization of a first neutral washing to purify biodiesel with high soap content is highly efficient since it abates a high percentage of these soaps without generating phase separation or problems of emulsion formation. Furthermore, this acid solution (water saturated with CO2) is less corrosive than the solutions normally used (hydrochloric, sulfuric, phosphoric, or citric acid). It was found that in order to decrease the sterol glucosides deposition in the final biodiesel, the combination of longer washing times and centrifugal separation was very effective in reducing these contaminants in the final product.","palabras_clave":"BIODIESEL|WATER WASHING|PURIFICATION|CARBON DIOXIDE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63601,"idioma_id":4,"titulo":"Differential Distribution of Plasmid Mediated Quinolone Resistance Genes in Clinical Enterobacteria with Unusual Phenotypes of Quinolone Susceptibility from Argentina","resumen":"We studied a collection of 105 clinical enterobacteria with unusual  phenotypes of quinolone susceptibility to analyze the occurrence of plasmid  mediated quinolone resistance (PMQR) and oqx  genes and their implications on quinolone susceptibility. The oqxA and oqxB genes were found in 31\/34 (91%) Klebsiella pneumoniae and 1\/3 Klebsiella  oxytoca. However, the oqxA\/oqxB-harboring isolates lacking other known  quinolone resistance determinants showed wide ranges of susceptibility to nalidixic  acid and ciprofloxacin. Sixty of the 105 (57%) isolates harbored at least one  PMQR gene (qnrB19, qnrB10, qnrB2, qnrB1, qnrS1 or aac(6´)-Ib-cr), belong to 8 enterobacterial species, were disseminated  throughout the country and most of them were categorized as susceptible by the  current clinical quinolone susceptibility breakpoints. We developed a disk  diffusion-based method to improve the phenotypic detection of aac(6\u2019)-Ib-cr. The most frequent PMQR genes in  our collection (qnrB19, qnrB10, aac(6\u2019)-Ib-cr)  were differentially distributed among enterobacterial species and two different  epidemiological settings were evident. First, the species associated to  community-acquired infections (Salmonella  spp. and Escherichia coli) mainly  harbored qnrB19 (unique PMQR gene) located  in small ColE1-type plasmids that might constitute its natural reservoirs. qnrB19 was not associated to an extended-spectrum-&#946;-lactamase  phenotype. Second, the species associated to hospital-acquired infections (Enterobacter spp., Klebsiella spp. and Serratia  marcescens) mainly harbored qnrB10  in ISCR1-containing class-1 integrons  that may also have aac(6\u2019)-Ib-cr as a cassette within the variable region. These two PMQR genes  were strongly associated to an extended-spectrum-&#946;-lactamase phenotype. Therefore,  this differential distribution of PMQR genes is strongly influenced by their  linkage or not to integrons.","palabras_clave":"PMQR|ENTEROBACTERIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63602,"idioma_id":4,"titulo":"Hysteresis properties of hexagonal arrays of FePd nanowires","resumen":"FePd nanowires (NWs) with different compositions havebeen grown into anodized aluminum oxide templates (AAO) by AC electrodepositionat room temperature. The effects of nanowire composition and morphology on thehysteresis properties of the ordered array of NWs are investigated. All the NWsare polycrystalline; the Fe-rich wires have a structure while the Pd rich onesare . FePd NWs are ferromagnetic and the spontaneous magnetization is found tobe parallel to the nanowire long axis (out of plane easy axis) and both, thesaturation polarization and the coercive field increase with the iron content","palabras_clave":"Alumina Template|FePd nanowires|hyysteresis properties|shape anisotropy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63603,"idioma_id":4,"titulo":"Evaluation of the protection exerted by Pisum sativum Ferredoxin-NADP(H) Reductase against injury induced by hypothermia on Cos-7 cells","resumen":"Hypothermia is employed as a method to diminish metabolism rates and preserve tissues and cells. However,low temperatures constitute a stress that produces biochemical changes whose extension depends on the duration and degree of cold exposure and is manifested when physiological temperature is restored. For many cellular types, cold induces an oxidative stress that is dependent on the elevation of intracellular iron, damages macromolecules, and is prevented by the addition of iron chelators. Pisum sativum Ferredoxin-NADP(H) Reductase (FNR) has been implicated in protection from injury mediated by intracellular iron increase and successfully used to reduce oxidative damage on bacterial, plant and mammalian systems. In this work, FNR was expressed in Cos-7 cells; then, they were submitted to cold incubation and iron overload to ascertain whether this enzyme was capable of diminishing the harm produced by these challenges. Contrary to expected, FNR was not protective and even exacerbated the damage under certain circumstances. It was also found that the injury induced by hypothermia in Cos-7 cells presented both iron-dependent and iron-independent components of damage when cells were actively dividing but only iron-independent component when cells were in an arrested state. This is in agreement with previousfindings which showed that iron-dependent damage is also an energy-dependent process.","palabras_clave":"HYPOTHERMIA|MAMMALIAN CELLS|PLANT FERREDOXIN REDUCTASE|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63604,"idioma_id":4,"titulo":"Synthesis of C-cinnamoyl glycosides and their inhibitory activity against mammalian carbonic anhydrases","resumen":"          A  small series of C-cinnamoyl glycosides incorporating the phenol moiety has been  prepared by reaction of glycosyl ketones with the appropiate benzaldehydes. &nbsp;Glycosides were tested for the inhibition of  twelve mammalian isoforms of carbonic anhydrase. This is the first study in  which a-CAs have been investigated for  their interaction with C-glycosides, a novel carbohydrate scaffold in the  design of carbonic anhydrase inhibitors. The C-cinnamoyl glycosides were generally effective CA inhibitors, with  inhibition constants in the low micromolar range against CA I, II, IV, VA, VB,  VI, VII, IX, XII, XIII, XIV and ineffective inhibitors of CA III. These results  confirm that attaching carbohydrate moieties to CA phenol pharmacophore improves  its inhibitory activity.          &nbsp;  ","palabras_clave":"C-GLYCOSIDES|CARBONIC ANHYDRASE|PHENOL|ENZYME INHIBITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63605,"idioma_id":4,"titulo":"'Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media'","resumen":"The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in culture medium, it is common the addition of growth-inducer. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by the adding of two growth-inducers, meso-inositol and tween 80, both at 0.5% in a traditional culture medium sabouraud glucose agar (SGA), and also in a traditional culture medium enriched with wheat flour and milk powder. From a traditional sterile culture of D. flagrans four groups were made: sabouraud glucose agar (SGA); sabouraud glucose agar meso-inositol 0.5 % (SGA-MI 0.5%); sabouraud glucose agar-tween 80 0.5% (SGA-TW8 0.5%) and sabouraud glucose agar - enriched (SGA-E). These media were placed at a constant temperature of 27ºC for four weeks. Following this, chlamydospores were gently rinsed off with sterile water and counted using a Neubauer haematocytometer to estimate the number of chlamydospores per ml of water. The addition of meso-inositol 0.5% to SGA promoted a significant increase (p<0.05) in chlamydospores production obtaining an average of 51,715,000 chlamydospores per Petri dish. The highest chlamydospores concentration was observed in SGA-E in comparison with SGA (p<0.01) obtaining an average of 208,760,000 chlamydospores. The aim of this study was to obtain basic knowledge regarding the effect of enriched culture medium and growth-inducing meso-inositol and tween 80 on micelial growth and production of chlamydospores","palabras_clave":"NEMATOPHAGOUS FUNGI|SOLID CULTURE MEDIA|BIOLOGICAL CONTROL|SPORULATION RATE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63606,"idioma_id":4,"titulo":"In vitro production of embryos in South American camelids","resumen":"Studies in reproductive biotechnology techniques have been minimal in South Americancamelids&nbsp; (SAC).&nbsp; Complex&nbsp; reproductive&nbsp; characteristics&nbsp; of&nbsp; these&nbsp; species&nbsp; contribute&nbsp; to&nbsp; slowprogress.&nbsp; Nevertheless,&nbsp; some&nbsp; techniques,&nbsp; such&nbsp; as&nbsp; in&nbsp; vitro&nbsp; fertilization,&nbsp; intracytoplasmicsperm injection and nuclear transfer have been applied and have produced advances in nowledge&nbsp; on&nbsp; embryo&nbsp; environment&nbsp; and&nbsp; in&nbsp; vitro&nbsp; conditions&nbsp; necessary&nbsp; for&nbsp; development.Embryo&nbsp; production&nbsp; may&nbsp; have&nbsp; a&nbsp; high&nbsp; impact&nbsp; in&nbsp; both&nbsp; domestic&nbsp; and&nbsp; wild&nbsp; camelids&nbsp; popula-tion.&nbsp; Studies&nbsp; addressed&nbsp; to&nbsp; improve&nbsp; in&nbsp; vitro&nbsp; embryo&nbsp; production&nbsp; and&nbsp; oocyte&nbsp; collection&nbsp; couldbe&nbsp; a&nbsp; potential&nbsp; key&nbsp; to&nbsp; develop&nbsp; IVF&nbsp; and&nbsp; embryo&nbsp; production&nbsp; as&nbsp; a&nbsp; routine&nbsp; procedure&nbsp; in&nbsp; camelids.","palabras_clave":"alpaca|llama|IVF|ICSI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63607,"idioma_id":4,"titulo":"PeV neutrinos from the propagation of ultra-high energy cosmic rays","resumen":"We discuss the possibility that the PeV neutrinos recently observed byIceCube are produced by the interactions of extragalactic cosmic raysduring their propagation through the radiation backgrounds. We show thatthe fluxes resulting from the decays of neutrons produced in theinteractions of cosmic ray protons with the CMB background aresuppressed (E&#957;2d&#934;&#957;\/dE &lt;10&#8209;10 GeV\/cm2 s sr), with those resultingfrom the decays of pions produced in the interactions with theUV\/optical\/IR backgrounds being the dominant ones at PeV energies. Theanti-neutrino fluxes produced by the decay of neutrons resulting fromthe photodisintegration of heavy nuclei with CMB photons are also shownto be quite suppressed(E&#957;2d&#934;&#957;\/dE &lt;10&#8209;11 GeV\/cm2 s sr), while those produced byphoto-pion processes with UV\/optical\/IR backgrounds may be larger,although they are not expected to be above those achievable in the pureproton case. Scenarios with mixed composition and low cutoff rigiditiescan lead to PeV neutrino fluxes enhanced with respect to those in thepure Fe scenarios. We also discuss the possible impact of the Glashowresonance for the detection of these scenarios, showing that it plays amoderate role.","palabras_clave":"cosmological neutrinos|ultra high energy cosmic rays|ultra high energy photons and neutrinos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63608,"idioma_id":4,"titulo":"Trypanosoma cruzi, the causative agent of Chagas disease, modulates interleukin-6-induced STAT3 phosphorylation via gp130 cleavage in different host  cells.","resumen":"Interleukin-6 mediates host defense and cell survival mainly through the activation of the transcription factor STAT3 via the glycoprotein gp130, a shared signal-transducing receptor for several IL-6-type cytokines. We have reported that the cardiotrophic parasite Trypanosoma cruzi protects murine cardiomyocytes from apoptosis. In agreement, an intense induction of the anti-apoptotic factor Bcl-2 is found in cardiac &#64257;bers during the acute phase of infection, establishing a higher threshold against apoptosis. We report here that inactive cruzipain, the main cysteine protease secreted by the parasite, speci&#64257;cally triggered TLR2 and the subsequent release of IL-6, which acted as an essential anti-apoptotic factor for cardiomyocyte cultures. Although comparable IL-6 levels were found under active cruzipain stimulation, starved cardiac cell monolayers could not be rescued from apoptosis. Moreover, cardiomyocytes treated with active cruzipain completely abrogated the STAT3 phosphorylation and nuclear translocation induced by recombinant IL-6. This inhibition was also observed on splenocytes, but it was reverted when the enzyme was complexed with chagasin, a parasite cysteine protease inhibitor. Furthermore, the inhibition of IL-6-induced p-STAT3 was evidenced in spleen cells stimulated with pre-activated supernatants derived from trypomastigotes. To account for these observations, we found that cruzipain enzymatically cleaved recombinant gp130 ectodomain, and induced the release of membrane-distal N-terminal domain of this receptor on human peripheral blood mononuclear cells. These results demonstrate, for the &#64257;rst time, that the parasite may modify the IL-6-induced response through the modulation of its cysteine protease activity, suggesting that  speci&#64257;c inhibitors may help to improve the immune cell activation and cardioprotective effects.","palabras_clave":"TRYPANOSOMA CRUZI|TLR2|IL-6 SIGNALING|GP130 IG-LIKE DOMAIN|HUMAN LEUKOCYTES|CARDIOMYOCYTES|CRUZIPAIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63609,"idioma_id":4,"titulo":"Development of magnetohydrodynamic modes during sawteeth in tokamak plasmas","resumen":"A dynamical analysis applied to a reduced resistive magnetohydrodynamics model is shown to explain the chronology of the nonlinear destabilization of modes observed in tokamak sawteeth. A special emphasis is put on the nonlinear self-consistent perturbation of the axisymmetric m&#8201;=&#8201;n&#8201;=&#8201;0 mode that manifests through the q-profile evolution. For the very low fusion-relevant resistivity values, the q-profile is shown to remain almost unchanged on the early nonlinear timescale within the central tokamak region, which supports a partial reconnection scenario. Within the resistive region, indications for a local flattening or even a local reversed-shear of the q-profile are given. The impact of this ingredient in the occurrence of the sawtooth crash is discussed.","palabras_clave":"PLASMA CONFINEMENT|PLASMA MAGNETOHYDRODYNAMICS|PLASMA INSTABILTY|TOKAMAK"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63610,"idioma_id":4,"titulo":"Bordetella pertussis iron regulated proteins as potential vaccine components","resumen":"Bordetella  pertussis  is  the  etiologic  agent  of  whooping  cough,  an  illness  whose  incidence  has  been  increasing  over  the  last  decades.  Pertussis  reemergence  despite  high  vaccination  coverage,  together  with  the recent  isolation  of  circulating  strains  de&#64257;cient  in  some  of  the  vaccine  antigens,  highlight  the  need  for  new vaccines.  Proteins  induced  under  physiological  conditions,  such  as  those  required  for  nutrient  acquisition during  infection,  might  represent  good  targets  for  better  preventive  strategies.  By  mean  of  serological proteome  analysis  we  identi&#64257;ed  two  novel  antigens  of  B.  pertussis  potentially  involved  in  iron  acquisition  during  host  colonization.  We  had  previously  demonstrated  that  one  of  them,  designated  IRP1-3, is  protective  against  pertussis  infection  in  mice.  In  the  present  study,  we  show  that  the  other  anti-  gen,  named  AfuA  (BP1605),  is  a  highly  antigenic  protein,  exposed  on  the  bacterial  surface,  conserved among  clinical  isolates  and  expressed  during  infection.  Immunization  of  mice  with  the  recombinant AfuA  induced  opsonophagocytic  antibodies  which  could  explain  the  protection  against  B.  pertussis  infection  conferred  by  mice  immunization  with  rAfuA.  Importantly,  we  found  that  the  addition  of  rAfuA  and rIRP1-3  proteins  to  the  commercial  three  pertussis  components  acellular  vaccine  signi&#64257;cantly  increased  its  protective  activity.  Taken  together,  our  results  point  at  these  two  antigens  as  potential  components of a new generation of acellular vaccines.","palabras_clave":"Bordetella pertussis|Vaccine|New antigens"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63611,"idioma_id":4,"titulo":"Violence as a barrier for HIV prevention among female sex workers in Argentina","resumen":"                  BACKGROUND:                   Violence against female sex workers (FSWs) has been increasingly reported as an important determinant of HIV infection risk. This study explores the frequency of different violent experiences (sexual abuse, rejection, beating and imprisonment) among FSWs in Argentina and its association with condom use and HIV and T. pallidum prevalence.                  METHODS:                   A convenience sample of 1255 FSWs was included in a cross-sectional study conducted between October 2006 and November 2009.                  RESULTS:                   Sexual abuse was reported by 24.1% (219\/907) of women. A total of 34.7% (42\/1234) reported rejection experiences, 21.9% (267\/1215) reported having been beaten and 45.4% (561\/1236) stated having been arrested because of their sex work activity. There was a higher frequency of inconsistent condom use with clients among FSWs who had experienced sexual abuse, rejection, and police detention. A higher frequency of HIV and T. pallidum infection was detected among FSWs who reported having been arrested by the police.                  CONCLUSION:                   The study shows for the first time the frequency of different violent situations among FSWs in Argentina. The association between violence against sex workers, condom use and STI prevalence demonstrated here calls for measures to reduce stigma and violence against FSWs. Such violent experiences may increase vulnerability to STI through coerced unprotected sex.","palabras_clave":"VIH|Trabajadoras sexuales femeninas|Violencia|Sífilis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63612,"idioma_id":4,"titulo":"(k, c)-coloring via Column Generation","resumen":"In this paper we study the (k; c)-coloring problem, a generalization of the well known Vertex Coloring Problem (VCP). We propose a new formulation and compare it computationally with another formulation from the literature. We also develop a diving heuristic that provides with good quality results at a reasonable computational e\u000Bort.","palabras_clave":"GRAPH COLORING|INTEGER PROGRAMMING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63613,"idioma_id":4,"titulo":"An exact DSatur-based algorithm for the Eq- uitable Coloring Problem","resumen":"This paper describes an exact algorithm for the Equitable Coloring Problem, basedon the well known DSatur algorithm for the classic Coloring Problem with newpruning rules specifically derived from the equity constraint. Computational experiencesshow that our algorithm is competitive with those known in literature.","palabras_clave":"equitable coloring|DSatur|exact algoritm"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63614,"idioma_id":4,"titulo":"A new species of Renealmia (Zingiberaceae) from Colombia","resumen":"Renealmia elianae (Zingiberaceae), a new species from the Central Andes of Colombia is described and illustrated. Renealmia elianae is morphologically close to R. puberula, differing by the inflorescence position, corolla Size, labellum texture, surface and color, and size of the epigynous glands.","palabras_clave":"ZINGIBERACEAE|RENEALMIA|TAXONOMY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63615,"idioma_id":4,"titulo":"Topological and Dimensional Synthesis of Planar Linkages for Multiple Kinematic Tasks","resumen":"This paper presents the combined use of two systematic methods for the synthesis of planar linkage mechanisms satisfying multiple kinematic tasks. First, a Graph Theory-based method is used to exhaustively enumerate the topological alternatives for a given problem. Then each feasible alternative is automatically dimensioned using the Precision Position Method; this computation includes space and design constraints. The existing methods to synthesize multiple tasks solve, in sequence, a decomposition of the problem into single kinematic tasks. The task decomposition and the topology selection for each task are usually performed by hand. This process leads to topologies with a repeated pattern and could lead to ignoring potentially desirable topologies. This paper analyzes a design strategy for the simultaneous solution of multiple kinematic tasks. This strategy has two advantages: (i) it eliminates the need for task decomposition, and (ii) it allows the exhaustive exploration of all non-isomorphic topologies up to a defined number of links. An example of simultaneous synthesis for a double rigid-body guidance task with application to a flap-tab mechanism is shown to illustrate the methodology.","palabras_clave":"Planar linkage mechanisms|Graph theory |Number synthesis |Modular dimensional synthesis |Multiple kinematic tasks |Synthesis strategies"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63616,"idioma_id":4,"titulo":"Molecular tools for cryptic Candida species identification with applications in a clinical laboratory","resumen":"Candida spp. includes more than 160 species but only 20 species pose clinical problems. C. albicans and C. parapsilosis account for more than 75% of all the fungemias worldwide. In 1995 and 2005, one C. albicans and two C. parapsilosis-related species were described, respectively. Using phenotypic traits, the identification of these newly described species is inconclusive or impossible. Thus, molecular-based procedures are mandatory. In the proposed educational experiment we have adapted different basic molecular biology techniques designed to identify these species including PCR, multiplex PCR, PCRbased restriction endonuclease analysis and nuclear ribosomal RNA amplification. During the classes, students acquired the ability to search and align gene sequences, design primers, and use bioinformatics software. Also, in the performed experiments, fungal molecular taxonomy concepts were introduced and the obtained results demonstrated that classic identification (phenotypic) in some cases needs to be complemented with molecular-based techniques. As a conclusion we can state that we present an inexpensive and well accepted group of classes involving important concepts that can be recreated in any laboratory.spp. includes more than 160 species but only 20 species pose clinical problems. C. albicans and C. parapsilosis account for more than 75% of all the fungemias worldwide. In 1995 and 2005, one C. albicans and two C. parapsilosis-related species were described, respectively. Using phenotypic traits, the identification of these newly described species is inconclusive or impossible. Thus, molecular-based procedures are mandatory. In the proposed educational experiment we have adapted different basic molecular biology techniques designed to identify these species including PCR, multiplex PCR, PCRbased restriction endonuclease analysis and nuclear ribosomal RNA amplification. During the classes, students acquired the ability to search and align gene sequences, design primers, and use bioinformatics software. Also, in the performed experiments, fungal molecular taxonomy concepts were introduced and the obtained results demonstrated that classic identification (phenotypic) in some cases needs to be complemented with molecular-based techniques. As a conclusion we can state that we present an inexpensive and well accepted group of classes involving important concepts that can be recreated in any laboratory.","palabras_clave":"CANDIDA|TAXONOMIA|MOLECULAR|METODOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63617,"idioma_id":4,"titulo":"HIV Knowledge and Beliefs among men who have sex with men (MSM) in Buenos Aires, Argentina.","resumen":"Five hundred men who have sex with men(MSM), recruited through respondent driven sampling inBuenos Aires, Argentina, were interviewed in order toassess knowledge and beliefs about HIV infection. Themean proportion of HIV correct knowledge answers was62 %; however participants whose sexual partners in theprior year included women (MSM&amp;W) had lower frequenciesof correct answers than participants with nowomen partners. Men with previous HIV testing experienceand those who were HIV positive had higher HIVknowledge. In relation to HIV beliefs, less than half ofparticipants responded correctly to each of the scenariospresented. Accurate answers for all items were more likelyamong those who only have sex with men. Men have basicHIV knowledge but also many misconceptions abouttransmission and prevention. Furthermore, MSM&amp;W haveless information than those who are exclusively MSM,probably related to the fact that information campaignsspecifically targeted gay identified men.","palabras_clave":"HIV KNOWLEDGE|MSM|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63618,"idioma_id":4,"titulo":"Changes in plant cover and functional traits induced by grazing disturbance in arid rangelands","resumen":"Grazing disturbance may affect the structure and functioning of arid rangelands. We analyzed the changes in plant cover and plant functional traits (plant height, SLA, N in green leaves) at the community, morphotype and species level in relation to grazing disturbance in arid ecosystems with more than 100 years of sheep grazing history. We identified two grazing areas and within each area we selected two representative and homogeneous sites located far (low grazing disturbance) and near (high grazing disturbance) from the single permanent watering point. We evaluated the plant cover at community, morphotype (evergreen tall shrubs, deciduous shrubs, dwarf shrubs, perennial herbs and perennial grasses) and species level at each site and randomly selected three individuals of modal size of each species to evaluate at them the selected plants traits. Plant cover was reduced by grazing disturbance at the community level. The cover of perennial grasses and evergreen tall shrubs decreased and that of dwarf shrubs increased with increasing grazing disturbance. Increasing cover of dwarf shrubs did not compensate the cover reduction of the other morphotypes. In contrast, plant height, SLA and N in green leaves were not affected by high grazing disturbance at community level as a consequence of positive and negative changes in these traits at morphotype and species levels induced by high grazing disturbance. We concluded that cover was the trait most affected by high grazing disturbance and positive and negative changes in other traits at plant morphotype or species level did not affect community attributes related to resistance against herbivory.","palabras_clave":"GRAZING DISTURBANCE|NITROGEN|PLANT COVER|PLANT HEIGHT|PLANT MORPHOTYPES|SPECIFIC LEAF AREA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63619,"idioma_id":4,"titulo":"Structure, Dynamics and Phase Behavior of Water in TiO2 Nanopores","resumen":"Mesoporous titania is a highly studied materialdue to its energy and environment-related applications, whichdepend on its tailored surface and electronic properties.Understanding the behavior of water in titania pores is acentral issue for practical purposes in photocatalysis, solar cells,bone implants, or optical sensors. In particular, themechanisms of capillary condensation of water in titaniamesopores and the organization and mobility of water as afunction of pore filling fraction are not yet known. In this work, molecular dynamics simulations of water confined in TiO2-rutilepores of diameters 1.3, 2.8, and 5.1 nm were carried out at various water contents. Water density and diffusion coefficients wereobtained as a function of the distance from the surface. The proximity to the interface affects density and diffusivity within adistance of around 10 Å from the walls, beyond which all properties tend to converge. The densities of the confined liquid in the2.8 and the 5.1 nm pores decrease, respectively, 7% and 4% with respect to bulk water. This decrease causes the watertranslational mobility in the center of the 2.8 nm pore to be appreciably larger than in bulk. Capillary condensation takes place inequilibrium for a filling of 71% in the 2.8 nm pore and in conditions of high supersaturation in the 5.1 nm pore, at a filling of65%. In the former case, the surface density increases uniformly with filling until condensation, whereas in the larger nanopore, acluster of water molecules develops on a localized spot on the surface for fillings just below the transition. No phase transition isdetected in the smaller pore. For all the systems studied, the first monolayer of water is strongly immobilized on the interface,thus reducing the accessible or effective diameter of the pore by around 0.6 nm. As a consequence, the behavior of water in thesepores turns out to be comparable to its behavior in less hydrophilic pores of smaller size.","palabras_clave":"titania|nanopores|molecular dynamics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63620,"idioma_id":4,"titulo":"Molecular and electronic structure of electroactive self-assembled monolayers","resumen":"Self-assembled monolayers (SAMs) containing electroactive functional groups are excellent modelsystems for the formation of electronic devices by self-assembly. In particular ferrocene-terminatedalkanethiol SAMs have been extensively studied in the past. However, there are still open questionsrelated with their electronic structure including the influence of the ferrocene group in the SAM-induced work function changes of the underlying metal. We have thus carried out a thorough exper-imental and theoretical investigation in order to determine the molecular and electronic structure offerrocene-terminated alkanethiol SAMs on Au surfaces. In agreement with previous studies we foundthat the Fc-containing alkanethiol molecules adsorb forming a thiolate bond with the Au surface witha molecular geometry 30&#9702; tilted with respect to the surface normal. Measured surface coverages in-dicate the formation of a compact monolayer. We found for the first time that the ferrocene grouphas little influence on the observed work function decrease which is largely determined by the alka-nethiol. Furthermore, the ferrocene moiety lies 14 Å above the metal surface covalently bonded tothe alkanethiol SAM and its HOMO is located at &#8722;1.6 eV below the Fermi level. Our results providenew valuable insight into the molecular and electronic structure of electroactive SAMs which are offundamental importance in the field of molecular electronics.","palabras_clave":"electron transfer|self-assembled monolayers|electronic structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63621,"idioma_id":4,"titulo":"Automatic dwelling segmentation of Buenos Aires Province for the 2010 Argentinian Census","resumen":"One of the logistical challenges in planning a population census is determining whichdwellings each enumerator must visit within a census tract. This is known as the dwellingsegmentation problem, which generally includes a set of constraints on the enumerators'assigned routes and various criteria regarding the homogeneity and uniformity of thesegmentation solutions. In this work, we present a computational approach for this prob-lem, which represents an improvement over manual methods and existing software. Theresulting method was successfully applied to the Province of Buenos Aires in the 2010Argentinian census.","palabras_clave":"integer programming|census|mathematical programming"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63622,"idioma_id":5,"titulo":"Variación anual e intrapoblacional de la dieta de Phymaturus cf. Palluma (Iguania: Liolaemidae en los Andes centrales en Argentina","resumen":"El conocimiento de los parámetros tróficos ayuda a comprender la historia natural de la especie, sus requerimientos ecológicos y estrategias involucradas en la búsqueda de alimento. Por ello, describimos y analizamos la dieta de Phymaturus cf. palluma y su variación en términos estacionales e  intrapoblacionales. Los ítems fueron identificados en términos cuali-cuantitativos. Se estimó el IRI según el volumen, numerosidad y frecuencia de ocurrencia de los ítems. Se calcularon porcentajes de material vegetal y parásitos nemátodos en tracto digestivo. Phymaturus cf. palluma presenta una alimentación herbívora basada en estructuras vegetales reproductivas y vegetativas. El ítem ?flores? fue fundamental, tanto en adultos como juveniles. En adultos macho y hembra, las flores de Adesmia pinnifolia fueron ítems fundamentales, sin embargo, la similitud cualitativa entre machos y hembras fue baja. Los machos presentaron mayor amplitud trófica respecto a hembras. Las estaciones de verano fueron las de mayor diversidad trófica. En cuanto al material vegetativo, hubo diferencias significativas sólo en el consumo de hojas entre estaciones. La competencia estacional por el recurso alimentario sería muy leve debido al amplio espectro de ítems encontrados en las distintas fechas. Phymaturus cf. palluma es un lagarto generalista que forrajea material vegetal en forma activa.","palabras_clave":"NICHO TRÓFICO|HERBOVORÍA|ESTRUCTURAS VEGETALES REPRODUCTIVAS|ESTRUCTURAS VEGETATIVAS|NEMÁTODOS|GENERALISMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63623,"idioma_id":4,"titulo":"THERMAL BIOLOGY OF LIOLAEMUS LIZARDS FROM THE HIGH ANDES: BEING EFFICIENT DESPITE ADVERSITY","resumen":"We studied the efficiency of thermoregulation in four high elevation Liolaemus species in the Andes of Salta, Argentina; L. irregularis, L. multicolor, L. albiceps and L. yanalcu. One of the species, L. irregularis, shows a broad distribution being in allopatry in some localities and in sympatry with L. albiceps, L. multicolor and L. yanalcu at different sites. Together with this variation in assemblages, the degree of phylogenetic relatedness is different with L. irregularis being most closely related to L. albiceps than to the other two species (L. multicolor and L. yanalcu). We measured body (Tb), microenvironmental (Ta, Ts), and operative temperatures (Te) in the field, and preferred body temperature (Tpref) in laboratory for each one of the species of assemblages. Three out of the four species showed a high thermoregulatory efficiency except for L. yanalcu, a moderate thermoregulator. The species studied here show high Tb in the field compared to most of the recorded Liolaemus species. However, the Tpref values were similar to other Liolaemus species. No evidence of thermal niche segregation between species in sympatry was observed. Our results suggest that the species studied here, despite of living at high elevation and harsh climatic conditions are able to behaviorally or physiologically thermoregulate to achieve Tbs close to their Tpref, probably because of low predation risk and perhaps low levels of competition.","palabras_clave":"LIOLAEMUS|THERMORAGULATION|SIMPATRY|ALLOPATRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63624,"idioma_id":4,"titulo":"Mathematical models for optimizing production chain planning in salmon farming","resumen":"The salmon farming production chain is structured in four consecutive phases: freshwater, seawater, plant processing, and distribution and marketing. The phases interact in a pull manner, freshwater stocks fish to meet seawater's demand, seawater produces to meet plant processing biomass demand, and the processing plant produces to satisfy consumers' demand. Freshwater planning decisions are in regard to which freshwater center the fish should be located depending on the state of development of the fish. The goal is to satisfy seawater's demand while minimizing costs. In the seawater phase, the fish are first placed in seawater centers, and then sent to the processing plant as they approach suitable harvest conditions. The goal of seawater is to maximize harvested biomass while satisfying processing plant's demand. This paper presents two mixed-integer linear programming models?one for the freshwater phase and another for the seawater phase. These models are designed in such a way that the production planning is well integrated and more efficient and incorporates the requirements of the farm operator's freshwater and seawater units (biological, economic, and health-related constraints) ensuring that production in both phases is better coordinated. The development of the two models was based on the farming operations of one of the main producer farms in Chile. Preliminary evaluations of the models indicate that they not only succeed in enforcing constraints that are difficult to be met by manual planning but also led to more effective results in terms of the objectives set out.","palabras_clave":"mathematical programming|integer programming|models"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63625,"idioma_id":4,"titulo":"An approach for efficient ship routing","resumen":"Ship routing problems are a particular kind of routing problems where the vehicles to be routed are vessels or ships, usually in maritime environments. In contrast to land routing, ship routing has unique features, including overnight trips, disjoint time windows, not necessarily prespecified routes, and a great uncertainty derived from weather conditions. In this work we present a special ship routing problem, which incorporates many features present in general ship routing settings. We discuss aspects related with data gathering and updating, which are particularly difficult in the context of ship routing. Additionally, we present a GRASP algorithm to solve this problem. We apply our solution approach to a salmon feed supplier based in southern Chile, and present computational results on real data.","palabras_clave":"mathematical programming|heuristics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63626,"idioma_id":4,"titulo":"The quasi-periodicity of the minority game revisited","resumen":"We analyze two well-known related aspects regarding the sequence of minority sides from the Minority Game (MGMG) in its symmetric phase: period-two dynamics and quasi-periodic behavior. We also study the sequence of minority sides in a general way within a graph-theoretical framework. In order to analyze the outcome dynamics of the MG, it is useful to define the MGpriorMGprior, namely an MGMG with a new choosing rule   of the strategy to play, which takes into account both prior preferences and game information. In this way, each time an agent is undecided because two of her best strategies predict different choices while being equally successful so far, she selects her a priori favorite   strategy to play, instead of performing a random tie-break as in the MGMG. This new choosing rule leaves the generic behavior of the model unaffected and simplifies the game analysis. Furthermore, interesting properties arise which are only partially present in the MGMG, like the quasi-periodic   behavior of the sequence of minority sides, which turns out to be periodic   for the MGprior.","palabras_clave":"Minority Game|Quasiperiodic Behaviour"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63627,"idioma_id":4,"titulo":"Heterogeneous Photoinduced Homolytic Aromatic Substitution of Electron-Rich Arenes with Perfluoroalkyl Groups in Water and Aqueous Media \u2013 A Radical-Ion Reaction","resumen":"The photoinduced electron transfer (PET) substitution reaction of electron rich aromatic nuclei with perfluoroalkyl groups was carried out in water or aqueous mixtures to render the substitution products resulting from replacement of aromatic H´s with the Rf moiety. Some mechanistic aspects are discussed, supporting the notion of a PET reaction leading to radical homolytic aromatic substitution (HAS) followed by an electron transfer (ET) and then a proton transfer (PT) sequence. A radical mechanism superimposed with a redox process is proposed to account for product formation. Evidence for the radical cation species (as an initiation event) generated from electron-rich arenes in the presence of perfluoroalkyl halides is provided by the UV transient spectra obtained by Nanosecond Laser Flash Photolysis techniques","palabras_clave":"aromatic perfluoroalkylation |electron transfer |green chemistry |homolytic aromatic substitution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63628,"idioma_id":4,"titulo":"BYSSAL ATTACHMENT ETCHINGS. A NEW BIOEROSION TRACE ON RECENT OYSTERS","resumen":"Byssate bivalves can be attached to hard substrates by byssal threads. Dissolution of the substrate to which they are attached may leave superficial bioerosion traces. This study reports the scars produced by byssus of Mytilus edulis and Aulacomya atra recorded in shells of Ostrea puelchana. Oyster valves were cut and gold-sputtered prior to scanning electron microscope observation. Each byssal thread leaves a characteristic scar on shells substrate. They etch an irregular trace on the substrate which may reach a diameter of several centimeters. These etching-traces comprise shallow round\/oval holes of variable number and placement. A bundle of pits corresponding to fibers that compose the thread core was identified in the interior of each hole. We suggest that this trace could be included in the ethological class Fixichnia. The description of this trace would allow adjusting the composition of fossil assemblages and therefore paleoenvironmental interpretations in those cases in which mytilid shells have not been preserved. The recognition of this trace along with other ones of tracemakers with reotaxis or positive phototaxis could be useful in inferring the life habit of fossil biogenic substrates and in reconstructing their taphonomic history.","palabras_clave":"OYSTERS|FIXICHNIA|BYSSAL ETCHINGS|SW ATLANTIC OCEAN|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63629,"idioma_id":4,"titulo":"Endemic tenebrionids (Coleoptera: Tenebrionidae) from the Patagonian steppe: a preliminary identification of areas of micro-endemism and richness hotspots","resumen":"Based on data from sixteen museum collections we listed the endemic tenebrionids that inhabit thePatagonian steppe. Then, according to the geographical location of the species, we identified areas ofmicro-endemism and hotspots within this biogeographic province. Results showed 115 endemic tenebrionidspecies from the Patagonian steppe, 25 areas of micro-endemism and five hotspots. We used these outcomesto identify areas of importance for conservation. Based on the comparison with other biogeographicprovinces, we suggest that the Patagonian steppe constitutes a center of high endemism for tenebrionids. Wehypothesize that the isolation of the Patagonian steppe from other arid areas would cause the highoccurrence of endemism. In addition, we suggest processes that could be responsible for the origin of theareas of micro-endemism and hotspots identified in this work.","palabras_clave":"ARGENTINA|ARID ENVIRONMENTS|BIODIVERSITY CONSERVATION|DARKLING BEETLES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63630,"idioma_id":4,"titulo":"The armadillo Propraopus sulcatus (Mammalia: Xenarthra) from the late Quaternary of northern Brazil and a revised synonymy with Propraopus grandis","resumen":"This paper describes new remains of Propraopus sulcatus from a late Quaternary cave deposit located in  Aurora doTocantins, northern Brazil. Propraopuswas recorded innumerous late Pleistoceneeearly Holocene  sites in South America, and its inclusiveness has been long debated. In order to address some of the  controversial taxonomic questions, the osteoderms of P. sulcatus were qualitatively and quantitatively  compared to those of related nominal species (Propraopus grandis, Propraopus magnus, Dasypus bellus, and  Dasypus punctatus); special attentionwas given to the former due to the debated synonymy between both  taxa. Analyses reveal that there is no morphologic, spatial, or temporal discontinuity between P. sulcatus and  P. grandis. Adopting morphologic, associational, and distributional criteria to define morphospecies, we  believe it is impossible to clearly separate both nominal entities. As a consequence, in agreement with  previous studies, we favor their synonymization. P. sulcatus has nomenclatural priority over P. grandis and  should be the validnamefor the species. The scarce analyzed remains referred to P. magnus showconcordant  size and morphology with P. sulcatus, but the analysis of more complete material is essential to determine its  synonymization. Finally, we revised and updated the geochronologic distribution of Propraopus.","palabras_clave":"Quaternary|Armadillo|Xenarthra|Brazil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63631,"idioma_id":4,"titulo":"Randomness of FSSM over GF(4) and quality of hopping turbo codes","resumen":"In this study, the authors study a turbo-coding (TC) scheme, whose constituent codes are designed using convolutional encoders. These encoders are finite-state sequence machines (FSSMs) operating over the Galois Field, GF(4). The scheme includes encryption polynomials whose coefficients are selected every L steps, from the set of optimal polynomials of GF(4). Two cases are considered for the polynomial selection: periodical and random. This kind of encoder was studied in a previous study and a correspondence between the randomness of the encoded sequence and performance of the TC was conjectured. The main contribution of this study is to systematically confirm this correspondence, by analysing the randomness of the output and performance of the TC using several randomness quantifiers. Three of the quantifiers are defined on the basis of recurrence plots. Other two quantifiers are defined on the basis of the information theory. All the quantifiers allow one to justify why the proposed TC works better with random selection of the optimal polynomials and with small values of L. In summary, it is shown that a random selection of polynomials and a small L produce FSSMs with enhanced randomness properties and it is also shown that they produce the best quality of the TC, measured by means of the corresponding bit error rate.","palabras_clave":"RANDOMNESS|FINITE STATE|SEQUENCE MACHINE|GALOIS FIELD|TURBO CODES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63632,"idioma_id":4,"titulo":"Aging modifies endocannabinoid 2-arachidonoylglycerol metabolism","resumen":"Abstract 2-Arachidonoylglycerol (2-AG) is one of the principal monoacylglycerol (MAG) species in animal tissues and the diacylglycerol lipase (DAGL) pathway the most important 2-AG biosynthetic pathway proposed to date. Lysophosphatidate phosphatase (LPAase) activity is part of another 2-AG-forming pathway in which monoacylglycerol lipase (MAGL) is the major degrading enzyme. The purpose of this study was to analyze the manner in which DAGL, LPAase and MAGL enzymes are modified in the central nervous system (CNS) during aging. To this end, diacylglycerols (DAGs) and MAGs of different composition were used as substrates of DAGL and MAGL, respectively. All enzymatic activities were evaluated in membrane and soluble fractions as well as in synaptic terminals from the cerebral cortex (CC) of adult and aged rats. Results related to 2-AG metabolism show that aging: a) decreases DAGLá expression in the membrane fraction whereas in synaptosomes it increases DAGLâ and decreases MAGL expression; b) decreases LPAase activity in both membrane and soluble fractions; c) decreases DAGL and stimulates LPAase activities in CC synaptic terminals; d) stimulates membrane-associated MAGL-coupled DAGL activity; and e) stimulates MAGL activity in CC synaptosomes. Our results also reveal that during aging the net balance between the enzymatic activities involved in 2-AG synthesis and breakdown is low availability of 2-AG in CC membrane fractions and synaptic terminals. Taken together, our results lead us to conclude that these enzymes play crucial roles in the regulation of 2-AG tissue levels during aging. ","palabras_clave":"2-ARACHIDONOYLGLYCEROL|CEREBRAL CORTEX|LYSOPHOSPHATIDIC ACID|SYNAPTOSOMES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63633,"idioma_id":5,"titulo":"Paleoflora de la Formación Paraná (Mioceno), Cuenca Chaco-Paranense, Argentina","resumen":"El registro fósil de fitolitos de palmeras afines a Trithrinax Mart. y Copernicia Mart. ex Endl., impresiones foliares afines a Ocotea Aublet., Paramyrciaria Kausel y Schinus L. y leños fósiles con afinidad a Anadenanthera Speg., Parapiptadenia Brenan, As­tronium Jack. y Solanum L. en el Mioceno Tardío de la Formación Paraná, soportan la idea que estos elementos tuvieron un área de distribución mayor en el pasado, y estarían indicando un clima más cálido y húmedo que el actual para esta región. La presencia de Anadenantheroxylon villaurquisense Brea, Aceñolaza y Zucol 2001 y Astroniumxylon portmannii Brea, Aceñolaza y Zucol 2001, dos leños fósiles afines a Anadenanthera colubrina var. cebil (Vell.) Brenan y Astronium urundeuva (Fr. Allem.) Engl respectivamente, permiten estimar que la distribución de los Bosques Estacionales Secos Neotropicales fue más amplia y austral durante el Mioceno.","palabras_clave":"MIOCENO SUPERIOR|PALEOFLORA| CUENCA CHACO-PARANENSE|PALEOECOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63634,"idioma_id":5,"titulo":"Age and gender dependent development of theory of mind in 6 to 8-years old children.","resumen":"The ability to attribute different mental states to distinct individuals, or Theory of Mind (ToM), is widely believed to be developed mostly during preschool years. How different factors such as gender, number of siblings or coarse personality traits affect this development is not entirely agreed upon. Here, we introduce a computerized version of the scaled ToM suite of tasks introduced by Wellman and Liu (2004), which allows us to meaningfully test ToM development on children 6 to 8-years old. We find that kids this age are still not entirely proficient in all ToM tasks, and continue to show a progression of performance with age. By testing this age range, too, we are able to observe a significant advantage of girls over boys in ToM performance. Other factors such as number of siblings, birth order, and coarse personality traits show no significant relation with the ToM task results. Finally, we introduce a novel way to quantify the scaling property of the suite involving a sequence of set inclusions and also, a comparison between specially tailored sets of logistic models. These measures confirm the validity of the scale in the 6 to 8-years old range.","palabras_clave":"Theory of Mind|Mental states|Development"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63635,"idioma_id":4,"titulo":"Synthesis and anticonvulsant activity of bioisosteres of trimethadione, N-derivative-1,2,3-oxathiazolidine-4- one-2,2-dioxides from a-hydroxyamides","resumen":"The synthesis and anticonvulsant activity of novel heterocycles N-derivative-1,2,3-oxathiazolidine-4-one-2,2-dioxides, bioisosteres of trimethadione (TMD, oxazolidine-2,4-dione) and phenytoin (PHE), are  described. TMD is an anticonvulsant drug widely used against absences seizures in the early 80\u2019s and PHE is an antiepileptic drug with a wide spectrum activity. The intermediates of synthesis of Nderivative-1,2,3-oxathiazolidine-4-one-2,2-dioxides, a-hydroxyamides, were obtained using microwave assisted synthesis. Anticonvulsant screening was performed in mice after intraperitoneal administration in the maximal electroshock seizure test (MES) and subcutaneous pentylenetetrazole seizures test  (scPTZ). These new compounds showed a wide spectrum activity and were no neurotoxic in the RotoRod test. a-Hydroxyamides and N-derivative-1,2,3-oxathiazolidine-4-one-2,2-dioxides were 3\u20134700 times more potent than valproic acid in the MES test. Quanti&#64257;cation of anticonvulsant protection was calculated (ED50) for the most active candidates; a-hydroxyamides 3a\u2013c and 3e, and N-derivative-oxathiazolidine-4-one-2,2-dioxides 5a\u2013c with ED50 values of 9.1, 53.9, 44.6, 25.2, 15.1, 91.1 and 0.06 mg\/kg,  respectively, in the MES test.","palabras_clave":"N-DERIVATIVE-1,2,3-OXATHIAZOLIDIN-4-ONE- 2,2-DIOXIDES|a-HYDROXYAMIDES|MICROWAVE SYNTHESIS|ANTICONVULSANT SCREENING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63636,"idioma_id":4,"titulo":"Synthesis and biological activity of divalent ligands based on 3-deoxy-4-thiolactose, an isosteric analogue of lactose","resumen":"We report here the synthesis of divalent ligands containing 3-deoxy-4-thiolactose. This thiodisaccharidehas been synthesized using the Michael addition of &#946;-1-thiogalactose to the &#945;,&#946;-unsaturated system ofsugar-derived dihydropyranones, followed by the reduction of the remaining carbonyl group. We wereable to control the configuration (S) of the stereocenter linked to sulfur (C-4) of the reducing end by conductingthe thioglycosylation at high temperature or by isomerization during the reduction of the2-ulose thiodisaccharide with NaBH4\/THF. The energy profile for this reaction on a model compound wascalculated. The anomeric position of the 3-deoxy-4-thiolactose was functionalized with a terminalalkyne, which was coupled to azide-containing sugar scaffolds through CuAAC reaction to affordmono- and divalent ligands. The final products were competitive inhibitors of E. coli &#946;-galactosidase inthe micromolar range. Their binding affinities to peanut agglutinin (PNA) were determined by isothermalcalorimetry, which showed a clear decrease in the Ka values for monovalent derivatives compared tolactose. This report contributes to establishing the role of a particular hydroxyl group of lactose in sugarproteinrecognition processes.","palabras_clave":"synthesis|click|ruthenium|PNA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63637,"idioma_id":4,"titulo":"Caffeic acid phenylethyl ester and MG132, two novel nonconventional chemotherapeutic agents, induce apoptosis of human leukemic cells by disrupting mitochondrial function","resumen":"The ability to modulate balance between cell survival and death is recognized for its great therapeutic potential. Therefore, research continues to focus on elucidation of cell machinery and signaling pathways that control cell proliferation and apoptosis. Conventional chemotherapeuticagents often have a cytostatic effect over tumor cells. New natural or synthetic chemotherapeutic agents have a wider spectrum of interesting antitumor activities that merit in-depth studies. In the present work, we aimed at characterizing the molecular mechanism leading to induction ofcell death upon treatment of the lymphoblastoid cell line PL104 with caffeic acid phenylethyl ester (CAPE), MG132 and two conventional chemotherapeutic agents, doxorubicine (DOX) and vincristine (VCR). Our results showed several apoptotic hallmarks such as phosphatidylserine (PS) exposure on the outer leaflet of the cell membrane, nuclear fragmentation, and increase sub-G1 DNA content after all treatments. In addition, all four drugs downregulated survivin expression.CAPE and both chemotherapeutic agents reduced Bcl-2, while only CAPE and MG132 significantly increased Bax level. CAPE and VCR treatment induced the collapse of mitochondrial membrane potential (&#916;&#968;m). All compounds induced cytochrome c release from mitochondrial compartment to cytosol. However, only MG132 caused the translocation of Smac\/DIABLO. Except for VCR treatment, all other drugs increased reactive oxygen species (ROS) productionlevel. All treatments induced activation of caspases 3\/7, but only CAPE and MG132 led to the activation of caspase 9. In conclusion, our results indicate that CAPE and MG132 treatmentof PL104 cells induced apoptosis through the mitochondrial intrinsic pathway, whereas the apoptotic mechanism induced by DOX and VCR may proceed through the extrinsic pathway.","palabras_clave":"Caffeic acid phenylethyl ester (CAPE) |MG132 |Mitochondria | Leukemia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63638,"idioma_id":4,"titulo":"Interplay between autophagy and apoptosis in pancreatic 5 tumors in response to gemcitabine","resumen":"Pancreatic cancer is an aggressive disease. Its incidencehas increased over the last two decades. It's currently the fourth cause ofdeath among cancers in the western world. Unfortunately, systemic chemotherapystill relies on just a few drugs which until now have produced unsatisfactoryresults. Gemcitabine (2\u2019-2\u2019-difluorodeoxycytidine) is currently the standardchemotherapy treatment at all stages of pancreatic adenocarcinoma. Survivalbenefit and clinical impact however remain moderate due to a high degree ofintrinsic and acquired resistance. Autophagy plays an important role incell death decision but can also protect cells from various apoptotic stimuli. Weinvestigated the function of autophagy in pancreatic carcinoma cells, which arefrequently insensitive tostandard chemotherapeuticagents. We previously explained that gemcitabine induced both autophagy andapoptosis in pancreatic tumor cells. We did not however describe therelationship between these two mechanisms. Here we demonstrate that autophagyis one of the mechanisms responsible for the refractory response of pancreatictumors to gemcitabine. We present evidence invitro and in vivo that proves autophagyplays a protective role in pancreatic ductal carcinoma cells, preventing themfrom entering the apoptotic pathway after stimulus with gemcitabine, thuscontributing to treatment resistance. A better understanding of the role in theprocess may help in the discovery of new strategies to overcome tumor drugresistance in this aggressive disease.","palabras_clave":"apoptosis|autophagy|drug resistance|pancreatic tumor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63640,"idioma_id":4,"titulo":"Cooperative effect of E2 and FGF2 on lactotroph proliferation triggered by signalling initiated at the plasma membrane","resumen":"In the present work we investigated the effect of 17â-estradiol (E2) and basic fibroblast growth factor (FGF2) on the lactotroph cell proliferative response and the related membrane-initiated signalling pathway. Anterior pituitary mixed cell cultures of random cycling 3-month-old female rats were treated with 10nM of E2, E2 membrane-impermeable conjugated (E2-BSA), PPT (ERá agonist) and DPN (ERâ agonist) alone or combined with FGF2 (10ng\/ml) for 30min or 4h. Although our results showed that the uptake of BrdU into the nucleus of lactotrophs was not modified by E2 or FGF2 alone, a significant increase in the lactotrophs uptake of BrdU was observed after E2\/FGF2 co-incubation, with this effect being mimicked by PPT\/FGF2. These proliferative effects were blocked by ICI182780 or PD98059. The involvement of membrane ER in the proliferative response of prolactin cells induced by the steroid and FGF2 co-incubation was confirmed using E2-BSA, and the association between ERá and FGF receptor was observed after E2\/FGF2 treatment by immunoprecipitation. A significant increase in the ERK1\/2 expression was noted after E2, E2-BSA, PPT and FGF2 alone, which was more noticeable after E2-BSA\/FGF-2, E2\/FGF2 or PPT\/FGF2 treatments. This study provides evidence that E2 and FGF2 exert a cooperative effect on the lactotroph proliferation principally by signalling initiated at the plasma membrane triggering a genomic effect mediated by MEK\/ERK1\/2, a common signalling pathway, which finally regulates the lactotroph population thus contributing to pituitary plasticity.","palabras_clave":"ESTRADIOL |FGF-2|LACTOTROPAS|ESTROGEN RECEPTORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63641,"idioma_id":4,"titulo":"RESTORATION OF THE NORMAL CLARA CELL PHENOTYPE AFTER CHRONIC ALLERGIC INFLAMMATION","resumen":"Bronchiolar Clara cells play a critical role in lung homeostasis. The main goal of this study was to evaluate the effects of chronic allergy on Clara cells and the efficacy of budesonide (BUD) and montelukast (MK) in restoring their typical phenotypes after OVA-induced chronic allergy in mouse. Their main defensive products, CC16 and SP-D, and the cytochrome CYP2E1 were evaluated and correlated with cell ultrastructure. Chronic allergy induced extensive bronchiolar metaplasia and normal Clara cell phenotype almost disappeared; instead, cells accumulated numerous big electron-lucent granules negative for CC16, and consequently the level of this anti-inflammatory protein was significantly reduced in BAL. A concomitant reduction of SP-D and CYP2E1 content was observed.   In spite of these profound phenotypic changes, they were pharmacologically reversed by both treatments applied, with a better efficiency being obtained for MK. This treatment decreased AB\/PAS positive content to control level, while after BUD it remained higher. In addition, most non-ciliated cells recovered their normal morphological features after MK whereas in BUD co-existed normal and ?transitional? cells with remnant mucous granules, and strongly stained for CC16 and SP-D. Accordingly, their levels were above normal values, while they were normalized by MK; similar differences were obtained for CYP2E1. Glucocorticoids were also less able to normalize neutrophils and may contribute to prolonged mucin expression.   These results show that chronic allergy-induced Clara cell metaplasia affects its own defensive mechanisms. In addition, anti-inflammatory treatments re-established Clara cells? functionality, with MK being more efficient at restoring a normal profile than BUD. This study highlights the role of epithelial cells in lung injuries and their contribution in anti-inflammatory therapies.","palabras_clave":"CC16|CLARA CELLS|ASTHMA TREATMENT|CHRONIC INFLAMMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63642,"idioma_id":4,"titulo":"Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation","resumen":"The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by other basic proteins called protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male pronucleus and syngamy. Decondensation is accomplished by protamine disulfide bond reduction by oocyte glutathione and replacement of protamines by oocyte histones with the aid of an acceptor molecule. Previous results from our laboratory have demonstrated that heparan sulfate present in the ooplasm functions as protamine acceptor during human sperm decondensation in vivo. In the present paper, we analyze the role of heparin, structural analogue of heparan sulfate, and dermatan sulfate in murine sperm chromatin decondensation in vitro, including the possibility of a synergistic effect between both glycosaminoglycans. Decondensation was assessed under phase contrast microscopy following incubation of murine spermatozoa with glutathione and either heparin, dermatan sulfate or a combination of both. Ultrastructural changes taking place during decondensation were analyzed by transmission electron microscopy. Both glycosaminoglycans were able to promote the decondensation of murine spermatozoa in vitro but the decondensing ability of heparin was significantly higher. Use of both glycosaminoglycans together revealed the existence of a synergistic effect. Transmission electron microscopy analysis of decondensing spermatozoa supported these findings. Synergism between heparin and dermatan sulfate was observed both in capacitated and non capacitated spermatozoa but decondensation kinetics was faster in the former. The results obtained indicate a new potential role for dermatan sulfate in murine sperm decondensation at fertilization and provide evidence of differences in the degree of chromatin condensation throughout the murine sperm nucleus.","palabras_clave":"chromatin decondensation| dermatan sulfate| heparin| murine spermatozoa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63643,"idioma_id":4,"titulo":"Functional group dominance and identity effects influence the magnitude of grassland invasion","resumen":"Variation in functional community composition is expected to influence the extent of exotic species invasions. Yet, whether resident functional groups control invasion through their relative biomass (mass ratio hypothesis) or by traits other than biomass (identity hypothesis) remains poorly understood.  We performed a 6-year experiment to determine the effects of removing different functional groups on exotic species biomass in a Flooding Pampa grassland, Argentina. Functional groups were defined by life-form (grasses or forbs), phenology (winter or summer) and origin (native or exotic). Removal of each functional group was compared against the removal of an equivalent amount of random biomass. Exotic group responses were monitored over 4 years of continuous removals, and after 2 years of recovery without manipulations.  Removal of dominant native summer grasses caused the greatest impact on exotic species and overall community composition. Native summer-grass removal significantly increased exotic grass (120%) and forb (730%) biomass beyond the level (46% and 180%, respectively) expected from deleting a similar amount of biomass at random. Exotic annual grasses showed only a transient increase, whereas exotic forb invasion persisted even after 2 years without removals.  Removing subordinate, native or exotic winter grasses, and rare native forbs significantly promoted exotic forbs, but to the same level (300%) as random biomass removals. Total grass removal increased exotic forbs to half the extent expected from adding the effects of single grass group removals. Dispersal limitation and harsh abiotic conditions may constrain exotic forb spread into such heavily grass-depleted patches.  Synthesis. The impact of losing a functional group on the magnitude and persistence of invasion reflected its relative contribution to community biomass. Identity attributes other than biomass (e.g. phenological niche) further enhanced the biotic control that dominant native grasses exerted on established exotic species. Our findings highlight the community legacies of past disturbances to dominant functional groups.","palabras_clave":"BIODIVERSITY|BIOTIC RESISTANCE|INVASION ECOLOGY|MASS RATIO HYPOTHESIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63644,"idioma_id":4,"titulo":"A Geodynamic View of Oil and Gas Resources Associated to the Unconventional Shale Reservoirs of Argentina","resumen":"Forecasting the presence of source rocks, reservoirs, seals, overburden rocks, as well as the development of traps  requires a thorough knowledge of the basin history. Sedimentary basins are the result of thermo-mechanical  processes acting on the lithosphere. At a general scale their infilling can then be divided into depositional sequences  according to different tectonics events, but in basin and petroleum modeling it is necessary to also consider climate  and sea level variations as fundamental controls in the evolution and location of the sedimentary sequences. A  geodynamic analysis will let us understand the complex interaction of the supply of sediments, the availability of the  accommodation space (both with tectonic components), sea level variations (which also may have a significant  tectonic influence) and\/or climate variations during basin filling and thus will give us significant tools for predicting  the petroleum system.  In Argentina, 545,000 km2 correspond to hydrocarbon producing basins known as Paleozoic, Cuyana, Austral,  Neuquén, Golfo San Jorge and Cretaceous, while the non-hydrocarbon producing ones cover 2,500,000 km2. The  associated source rocks are eleven, all of it with potential to be unconventional reservoirs. A situation that is  becoming true in view of the results obtained from drilling more than 100 wells in the past two years. Five of them  are marine in origin while six were deposited in lacustrine environments with ages ranging from Late Devonian to  Early Cretaceous. Average TOC are in between 0.5 to 11 (wt%) with variable thicknesses up to 2,000 meters.  Drilled wells targeting these objectives have a total depth that varies from 2,000 down to 5,000 meters. The aim of  the present contribution is to propose a dynamic modeling of the geological processes occurred in the productive  basins of Argentina over geological time spans to better understand the existing unconventional reservoirs as volume  estimations assign 774 TCF of technically recoverable resources (EIA, 2011).","palabras_clave":"Unconventional|Shale oil\/gas|geodynamic|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63645,"idioma_id":4,"titulo":"Human leukaemic cell lines synthesize hyaluronan to avoid  senescence and resist chemotherapy","resumen":"Hyaluronan (HA) is one of the major components of the extracellular matrix. Several solid tumors produce high levels of HA, which promotes survival and multidrug resistance (MDR). HA oligomers (oHAs) can block HA effects. However, little is known about the role of HA in hemato-logical malignancies. The aim of this work was to determine whether HA or its oligomers can modulate the proliferation of leukemia cells as well as their effect on MDR. Receptors and signaling pathways involved were also analyzed. For this purpose, the human leukemic cell lines K562 and Kv562, which are sensitive and resistant to Vincristine (VCR), respectively, were used. We demonstrated that HA induced cell proliferation in both cell lines. On K562 cells, this effect was mediated by cluster differentiation 44 (CD44) and activation of both phosphoinositide 3-kinase (PI3K)\/protein kinase B (Akt) and mitogen-activated protein kinase kinase (MEK)\/extracellular signal-regulated kinase (ERK) pathways, whereas on Kv562 cells, the effect was mediated by receptor for hyaluronan-mediated motility (RHAMM) and PI3K\/Akt activation. The inhibition of HA synthesis by 4-methylumbelliferone (4MU) decreased cell line proliferation and sensitized Kv562 to the effect of VCR through P-glycoprotein (Pgp) inhibition, in both cases with senescence induction. Moreover, oHAs inhibited K562 proliferation mediated by CD44 as well as Akt and ERK down-regulation. Furthermore, oHAs sensitized Kv562 cells to VCR by Pgp inhibition inducing senescence. We postulate that the synthesis of HAwould promote leukemia progression mediated by the triggering of the above-mentioned proliferative signals. These findings highlight the potential use of oHAs and 4MU as coadjuvant for drug-resistant leukemia. © The Author 2013.","palabras_clave":"Hyaluronan|CD44|RHAMM|Senescence|Drug resistance|Leukaemia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63646,"idioma_id":4,"titulo":"ENVIRONMENTAL PESTICIDE EXPOSURE MODULATES CYTOKINES, ARGINASE AND ORNITHINE DECARBOXYLASE EXPRESSION IN HUMAN PLACENTA","resumen":"  To evaluate the citokine balance and enzymatic alterations induced by environmental pesticide exposure during pregnancy, this transversal study explored placentas derived from non.exposed womwn and from women living in a rural área, collected during intensive organophsophate pesticide spraying season and during non-spreying season.    The exposure bionarkers blood cholinesterase and placental carboxylesterase were significantly decreased. Among the citokines studied IL-8 IL-6 IL-10 and IL-13 the expression frequency of IL-13 increases.    arginase and ornithine decarboxilase enzymes were induced in SCT and endotelial cells. These findings suggest that environmental pesticide exposure impacts the placenta by increasing the expression frequency of the anti-inflammatory cytokine IL-13, wich may be related to the up-regulation of enzymes implicated in tissue repair","palabras_clave":"placenta|cytokines|arginase|ornithine decarboxylase"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63647,"idioma_id":4,"titulo":"Amphibole in vermiculite mined in Argentina. Morphology, quantitative and chemical studies on the different phases of production. Their environmental impact","resumen":"Vermiculites with impurities of amphibole, a  dry fine residue and calcined materials from three deposits  in the province of Co´rdoba, Argentina, were studied. From  a commercial perspective, amphiboles are considered as  ??asbestos??, a group of silicate minerals with strong and  flexible fibres that are heat resistant and chemically inert,  and thus well suited for heat insulation. These fibrous  particles have provoked controversies about the toxicity  impact on human health and the development of diseases  such as mesothelioma, asbestosis or lung cancer. Their  commercialization and exploitation are currently prohibited.  The sampled minerals were identified with a petrographic  microscope, chemical analyses, SEM and XRD. It  was concluded that amphibole minerals are present in all  the deposits studied and in every phase of plant production,  but not all of them have asbestiform characteristics.","palabras_clave":"amohibole|vermiculite|asbesto|Córdoba"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63648,"idioma_id":4,"titulo":"Experimental infection with Trichinella T12 in domestic cats","resumen":"Trichinella spiralis has been documented in wild animals in Argentina, including puma, armadillos, rats and wild boars. In 2008, molecular analysis identified Trichinella T12 from a naturally infected puma (Puma concolor) from Patagonia. The aim of the present work was to study the relationship between the infectivity and pathology of Trichinella T12 in the puma and in domestic cats, and the possible risks that may be present for transmission among these animals. Two cats (A and B) were orally-infected with 3300 and 1850 Trichinella T12 muscle larvae, respectively; one additional cat was used as a control. During the 54 days post-infection, a daily examination was performed which included monitoring body temperature, and cardiac and respiration rates; the animals were then euthanized. Hematological studies included hematocrit (%), hemoglobin (g\/dl), and white cell, neutrophil, lymphocyte and eosinophil counts. Blood biochemistry included urea, creatinine, AST, ALT, CK, LDH and ALP. An ELISA assay was also performed. At necropsy, organs (liver, spleen, brain, cerebellum and kidney), nails and muscle samples were obtained for histopathology studies and artificial digestion. The muscles that were studied included the diaphragm, massetter, cutaneous, temporal, intercostals, lumbar, tongue, limbs, neck and tail. Clinical signs, such as anorexia, diarrhea, vomiting, shaggy hair, decay and muscle pain, were observed in both cats. The eosinophil counts were elevated in both cats A and B. Trichinella larvae were recovered from all of the muscles analyzed where the histopathology showed larvae in several muscles without degenerative reaction. Neither larvae nor lesions were observed in non-muscular organs. Cat A had a maximum of 246 larvae per gram (lpg) in the temporal muscle and a minimum of 80 lpg in the tongue, while cat B had a maximum of 65 lpg in muscles of the leg and a minimum of 10 lpg in tail muscles. This study represents the first record of experimental infection of cats with Trichinella T12.","palabras_clave":"TRICHINELLA T12|DOMESTIC CATS|EXPERIMENTAL |INFECTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63649,"idioma_id":4,"titulo":"Physical Properties, Star Formation and Active Galactic Nucleus Activity in Balmer Break Galaxies at 0<z<1.","resumen":"We present a spectroscopic study with the derivation of the physical properties of 37 Balmer break galaxies,which have the necessary lines to locate them in star-forming\u2013active galactic nuclei (AGNs) diagnostic diagrams.These galaxies span a redshift range from 0.045 to 0.93 and are somewhat less massive than similar samples ofprevious works. The studied sample has multiwavelength photometric data coverage from the ultraviolet to mid-infrared (MIR) Spitzer bands. We investigate the connection between star formation and AGN activity via optical,mass-excitation (MEx), and MIR diagnostic diagrams. Through optical diagrams, 31 (84%) star-forming galaxies,two (5%) composite galaxies, and three (8%) AGNs were classified, whereas from the MEx diagram only onegalaxy was classified as AGN. A total of 19 galaxies have photometry available in all the IRAC\/Spitzer bands. Ofthese, three AGN candidates were not classified as AGN in the optical diagrams, suggesting they are dusty\/obscuredAGNs, or that nuclear star formation has diluted their contributions. By fitting the spectral energy distribution ofthe galaxies, we derived the stellar masses, dust reddening E(B &#8722; V ), ages, and UV star formation rates (SFRs).Furthermore, the relationship between SFR surface density (&#931;SFR ) and stellar mass surface density per time unit(&#931;M&#8727; \/&#964; ) as a function of redshift was investigated using the [O ii] &#955;3727, 3729, H&#945; &#955;6563 luminosities, whichrevealed that both quantities are larger for higher redshift galaxies. We also studied the SFR and specific SFR(SSFR) versus stellar mass and color relations, with the more massive galaxies having higher SFR values but lowerSSFR values than less massive galaxies. These results are consistent with previous ones showing that, at a givenmass, high-redshift galaxies have on average larger SFR and SSFR values than low-redshift galaxies. Finally, bluergalaxies have larger SSFR values than redder galaxies and for a given color the SSFR is larger for higher redshiftgalaxies.","palabras_clave":"galaxies: active|galaxies: evolution|galaxies: high redshift|galaxies: nuclei"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63650,"idioma_id":4,"titulo":"Host Cell Poly(ADP-ribose) glycohydrolase Is Crucial for Trypanosoma cruzi  Infection Cycle","resumen":"Trypanosoma cruzi, etiological agent of Chagas´ disease, has a complex life cycle which involves the invasion of mammalian host cells, differentiation and intracellular replication. Here we report the first insights into the biological role of a poly(ADPribose) glycohydrolase in a trypanosomatid (TcPARG). In silico analysis of the TcPARG gene pointed out the conservation of key residues involved in the catalytic process and, by Western blot, we demonstrated that it is expressed in a life stagedependant manner. Indirect immunofluorescense assays and electron microscopy using an anti-TcPARG antibody showed that this enzyme is localized in the nucleus independently of the presence of DNA damage or cell cycle stage. The addition of poly(ADP-ribose) glycohydrolase inhibitors ADP-HPD (adenosine diphosphate (hydroxymethyl)pyrrolidinediol) or DEA (6,9-diamino-2-ethoxyacridine lactate monohydrate) to the culture media, both at a 1 &#956;M concentration, reduced in vitro epimastigote growth by 35% and 37% respectively, when compared to control cultures.  We also showed that ADP-HPD 1 &#956;M can lead to an alteration in the progression of the cell cycle in hydroxyurea synchronized cultures of T. cruzi epimastigotes. Outstandingly, here we demonstrate that the lack of poly(ADP-ribose) glycohydrolase activity in Vero and A549 host cells, achieved by chemical inhibition or iRNA, produces the reduction of the percentage of infected cells as well as the number of amastigotes per cell and trypomastigotes released, leading to a nearly complete abrogation of the infection process. We conclude that both, T. cruzi and the host, poly(ADP-ribose) glycohydrolase activities are important players in the life cycle of Trypanosoma cruzi, emerging as a promising therapeutic target for the treatment of Chagas´ disease.","palabras_clave":"POLY(ADP-RIBOSE)GLYCOHYDROLASE|TRYPANOSOMA CRUZI|CELL INFECTION|PARG INHIBITORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63651,"idioma_id":5,"titulo":"Aplicación del método de Lattice Boltzmann a la simulación de obstrucciones complejas fácilmente penetrables","resumen":"Se presenta la aplicación del Método de Lattice Boltzmann (LBM) a la simulación de flujos a través de obstrucciones complejas fácilmente penetrables. En particular nos interesa la simulación de flujos laminares estacionarios así como de flujos que presentan oscilaciones periódicas generadas por inestabilidades del flujo laminar. Para la simulación del flujo a través del medio poroso se utilizó un esquema originalmente desarrollado para bordes inmersos en el dominio de cálculo (IB - Immersed Boundary). Para validar los resultados numéricos se contrastaron con mediciones experimentales realizadas con anemometría térmica en un canal de aire y con los resultados de un análisis de estabilidad lineal. Los resultados de las simulaciones en condiciones de flujo estacionario, es decir a bajos números de Reynolds, muestran buena coincidencia con los resultados experimentales. Se pudo observar que el cambio a un modo de flujo con oscilaciones ocurre a un número de Reynolds por encima del valor crítico obtenido del análisis de estabilidad lineal. Finalmente se compararon los campos de las fluctuaciones de velocidad con el modo más inestable obtenido del análisis de estabilidad lineal, pudiendo observar un excelente acuerdo entre ambos.","palabras_clave":"Lattice Boltzmann|Obstrucciones complejas|Medio poroso|Frontera inmersa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63652,"idioma_id":4,"titulo":"Recurrent Jellyfish Blooms are a Consequence of Global Oscillations","resumen":"A perceived recent increase in global jellyfish abundance has been portrayed as a symptom of degraded oceans. This perception is based primarily on a few case studies and anecdotal evidence, but a formal analysis of global temporal trends in jellyfish populations has been missing. Here, we analyze all available long-term datasets on changes in jellyfish abundance across multiple coastal stations, using linear and logistic mixed models and effect-size analysis to show that there is no robust evidence for a global increase in jellyfish. Although there has been a small linear increase in jellyfish since the 1970s, this trend was unsubstantiated by effect-size analysis that showed no difference in the proportion of increasing vs. decreasing jellyfish populations over all time periods examined. Rather, the strongest nonrandom trend indicated jellyfish populations undergo larger, worldwide oscillations with an approximate 20-y periodicity, including a rising phase during the 1990s that contributed to the perception of a global increase in jellyfish abundance. Sustained monitoring is required over the next decade to elucidate with statistical confidence whether the weak increasing linear trend in jellyfish after 1970 is an actual shift in the baseline or part of an oscillation. Irrespective of the nature of increase, given the potential damage posed by jellyfish blooms to fisheries, tourism, and other human industries, our findings foretell recurrent phases of rise and fall in jellyfish populations that society should be prepared to face.","palabras_clave":"DECADAL CYCLES|SYNCHRONY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63653,"idioma_id":4,"titulo":"Biodiversity and distribution patterns of planktonic cnidarians in San Matías Gulf, Patagonia, Argentina","resumen":"The special location (40 - 42 ºS in the Southwestern Atlantic ocean) and hydrodynamic regime (limited water exchange with open ocean) in San Matías Gulf (Argentina) seem leading to a particular fauna of planktonic cnidarians where their abundances are mainly shaped by the Gulf circulation patterns. Four oceanographic cruises, covering 93 stations in 3 different seasons during 2007 and 2008 were carried out in order to quantify species richness, abundance as well as to analyse its distributions.  Twenty 20 species of hydromedusae and 1 siphonophore were identified, increasing the total number of hydromedusae for the area to 23, s. A similar value to the one found in the abutting Argentine continental shelf (20) but with a different assemblage composition. Generally, Hhydromedusae abundances found were low except for a bloom of the leptomedusa Obelia spp., during the cold season. The only siphonophore (Pyrostephos vanhoeffeni) found in the area has previously been thought to be endemic to Antarctic and Subantarctic waters, this being the first record for temperate waters of the Southwest Atlantic Ocean.","palabras_clave":"HYDROMEDUSAE|SIPHONOPHORAE|SAN MATÍAS GULF"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63654,"idioma_id":4,"titulo":"Routine HIV testing among hospitalized patients in Argentina. Is it time for a policy change?","resumen":"Introduction: The Argentinean AIDS Program estimates that 110,000 persons are living with HIV\/AIDS in Argentina. Of those, approximately 40% are unaware of their status, and 30% are diagnosed in advanced stages of immunosuppression. Though studies show that universal HIV screening is cost-effective in settings with HIV prevalence greater than 0.1%, in Argentina, with the exception of antenatal care, HIV testing is always client-initiated.  Objective: We performed a pilot study to assess the acceptability of a universal HIV screening program among inpatients of an urban public hospital in Buenos Aires.  Methods: Over a six-month period, all eligible adult patients admitted to the internal medicine ward were offered HIV testing. Demographics, uptake rates, reasons for refusal and new HIV diagnoses were analyzed.  Results: Of the 350 admissions during this period, 249 were eligible and subsequently enrolled. The enrolled population was relatively old compared to the general population, was balanced on gender, and did not report traditional high risk factors for HIV infection. Only 88 (39%) reported prior HIV testing. One-hundred and ninety (76%) patients accepted HIV testing. In multivariable analysis only younger age (OR 1.02; 95%CI 1.003-1.05) was independently associated with test uptake. Three new HIV diagnoses were made (undiagnosed HIV prevalence: 1.58%); none belonged to a most-at-risk population.  Conclusions:  Our findings suggest that universal HIV screening in this setting is acceptable and potentially effective in identifying undiagnosed HIV-infected individuals.  If confirmed in a larger study, our findings may inform changes in the Argentinean HIV testing policy.","palabras_clave":"voluntary universal HIV testing|provider-initiated HIV testing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63655,"idioma_id":4,"titulo":"Early Gag Immunodominance of the HIV-Specific T-Cell Response during Acute\/Early Infection Is Associated with Higher CD8","resumen":"The important role of the CD8 T-cell response on HIV control is well established. Moreover, the acute phase of infection representsa proper scenario to delineate the antiviral cellular functions that best correlate with control. Here, multiple functionalaspects (specificity, ex vivo viral inhibitory activity [VIA] and polyfunctionality) of the HIV-specific CD8 T-cell subset arisingearly after infection, and their association with disease progression markers, were examined. Blood samples from 44 subjectsrecruited within 6 months from infection (primary HIV infection [PHI] group), 16 chronically infected subjects, 11 elite controllers(EC), and 10 healthy donors were obtained. Results indicated that, although Nef dominated the anti-HIV response duringacute\/early infection, a higher proportion of early anti-Gag T cells correlated with delayed progression. Polyfunctional HIV-specificCD8 T cells were detected at early time points but did not associate with virus control. Conversely, higher CD4 T-cell setpoints were observed in PHI subjects with higher HIV-specific CD8 T-cell VIA at baseline. Importantly, VIA levels correlatedwith the magnitude of the anti-Gag cellular response. The advantage of Gag-specific cells may result from their enhanced abilityto mediate lysis of infected cells (evidenced by a higher capacity to degranulate and to mediate VIA) and to simultaneously produceIFN-. Finally, Gag immunodominance was associated with elevated plasma levels of interleukin 2 (IL-2) and macrophageinflammatory protein 1 (MIP-1). All together, this study underscores the importance of CD8 T-cell specificity in the improvedcontrol of disease progression, which was related to the capacity of Gag-specific cells to mediate both lytic and nonlyticantiviral mechanisms at early time points postinfection.","palabras_clave":"CTL   |HIV|Acute Infection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63656,"idioma_id":4,"titulo":"Supervised Constrained Optimization of  Bayesian Non-local Means Filter with Sigma","resumen":"Speckle reduction is an important problem in synthetic aperture radar   (SAR) image analysis. Recent years have seen how Bayesian filters emerge   as the natural extension of the nonlocal means filters, providing a   general framework to deal with multiplicative (speckle) noise. In this   paper, we present an easy-to-use software tool applying an evolutionary   algorithm to optimize a Bayesian nonlocal means filter with sigma   preselection for denoising SAR images. The desired result is a filtered   image having a significative reduction in its variance but preserving   the original mean value of the noisy image. A mixed-integer constrained   optimization problem is stated and solved with the human intervention,   where the user assists the evolutionary algorithm to reduce the noisy   image variance under the restriction of keeping the mean value of the   noisy SAR image within a predetermined interval of acceptance. We apply   the methodology to a set of synthetic and real SAR speckle corrupted   images. The results through the evaluation of objective global and local   quality criteria show the excellent potential of the proposal.","palabras_clave":"evolutionary computation|image denosising|radar imaging|Syntethic Aperture Radar"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63657,"idioma_id":4,"titulo":"A Public Health Response against Strongyloides stercoralis : Time to Look at Soil-Transmitted Helminthiasis in Full","resumen":"Strongyloides stercoralis infections have aworldwide distribution with a global burden in terms ofprevalence and morbidity that is largely ignored. A publichealth response against soil-transmitted helminth (STH)infections should broaden the strategy to include S.stercoralis and overcome the epidemiological, diagnostic,and therapeutic challenges that this parasite poses incomparison to Ascaris lumbricoides, Trichuris trichiura, andhookworms. The relatively poor sensitivity of single stoolevaluations, which is further lowered when quantitativetechniques aimed at detecting eggs are used, alsocomplicates morbidity evaluations and adequate drugefficacy measurements, since S. stercoralis is eliminated instools in a larval stage. Specific stool techniques for thedetection of larvae of S. stercoralis, like Baermann?s andKoga?s agar plate, despite superiority over direct techniques are still suboptimal. New serologies using recombinant antigens and molecular-based techniques offernew hopes in those areas. The use of ivermectin ratherthan benzimidazoles for its treatment and the need tohave curative regimens rather than lowering the parasiteburden are also unique for S. stercoralis in comparison tothe other STH due to its life cycle, which allowsreproduction and amplification of the worm burdenwithin the human host. The potential impact on STH ofthe benzimidazoles\/ivermectin combinations, alreadyused for control\/elimination of lymphatic filariasis, shouldbe further evaluated in public health settings. Whilewaiting for more effective single-dose drug regimens andnew sensitive diagnostics, the evidence and the toolsalready available warrant the planning of a commonplatform for STH andS. stercoralis control.","palabras_clave":"STRONGYLOIDES|SOIL-TRANSMITTED-HELMINTHS|ARGENTINA|MORBIDITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63658,"idioma_id":5,"titulo":"FRACCION EROSIONABLE DEL SUELO EN EL OESTE BONAERENSE BAJO SISTEMAS DE USO DE LA TIERRA CONTRASTANTES","resumen":"La región Sub-húmeda Pampeana (RSP) posee características edafo-Climáticas que le confieren a sus tierras una gran fragilidad al proceso de degradación por erosión eólica. La expresión de dicho proceso dependerá de la implementación de aquellos usos de la tierra que favorezcan la presencia en superficie de la fracción de agregados erosionables (FE). En suelos con escaso contenido de arcilla, como los encontrados en la RSP, la materia orgánica edáfica (MO) es un elemento importante para el mantenimiento de estructuras estables. En la RSP son escasos los estudios a escala de lote, acerca de cómo es la distribución  espacial de la FE bajo usos contrastantes de la tierra. Los objetivos del presente trabajo son: 1) comparar la FE y el contenido de MO superficial de suelos correspondientes a dos lotes ubicados en el oeste bonaerense, con sistemas de uso de la tierra contrastantes; 2) investigar para los dos usos de la tierra estudiados, el grado de vinculación entre ambas variables; 3) evaluar y contrastar su distribución en el espacio en los dos lotes bajo análisis. El trabajo se realizó en el partido de Trenque Lauquen, sobre situaciones bajo uso ganadero y agrícola. En ambas se tomaron muestras a intervalos regulares, mediante un diseño en cuadrícula. Se determinó la FE y MO, y se compararon los resultados de estas variables entre usos de la tierra, confeccionándose además los respectivos mapas de distribución espacial. El uso ganadero determinó valores de FE más bajos y de MO más altos, respecto al agrícola. Ambas propiedades se vincularon significativamente sólo bajo el primer uso. Los mapas de distribución espacial de FE y MO de ambos lotes mostraron patrones similares. Se pudo comprobar que la expresión de la  fragilidad a la erosión eólica puede ser controlada a través del uso de la tierra, aunque dichos cambios son condicionados por la heterogeneidad edáfica intrínseca heredada.","palabras_clave":"EROSIÓN EÓLICA|USO DE LA TIERRA|MATERIA ORGÁNICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63659,"idioma_id":4,"titulo":"Use of clomipramine as chemotherapy of the chronic phase of  Chagas disease","resumen":"Chagas infection is a major endemic disease affecting Latin American countries. The persistence of Trypanosoma cruzi generates a chronic inflammatory reactivity that induces an immune response directed to the host´s tissues. The effectiveness of the treatment in the chronic phase is still unsatisfactory due, amongst other reasons, to the collateral effects of the drugs used. We investigated the effect of clomipramine, a tricyclic antidepressant that, when used as a treatment of T. cruzi-chronically infected mice, inhibits trypanothione reductase, an exclusive and vital enzyme of T. cruzi. Clomipramine improved survival (P<0.05) by diminishing the parasite intensity as demonstrated by PCR studies in the heart and skeletal muscle, and significantly prevented the evolution to fibrosis of the inflammatory infiltrates. Clomipramine could be a good candidate for the treatment of chronic Chagas disease.","palabras_clave":"CHAGAS DISEASE|TREATMENT|TRYPANOTHIONE REDUCTASE|CLOMIPRAMINE|CARDIAC CHRONIC STAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63660,"idioma_id":4,"titulo":"Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein","resumen":"Citrus psorosisvirus (CPsV) and Mirafiori lettucebig-veinvirus (MiLBVV),membersofthe Ophioviridae  family,havesegmentednegative-sensesingle-strandedRNAgenomes.Todatenoreportshavedescribed  how ophiovirusesspreadwithinhostplantsand\/ortheproteinsinvolvedinthisprocess.Hereweshow  that the54KproteinofCPsVisencodedbyRNA2anddescribeitssubcellulardistribution.Upontransient  expressionin Nicotiana benthamiana epidermal cellsthe54Kprotein,andalsoits54Kcounterpart  proteinofMiLBVV,localizetoplasmodesmataandenhanceGFPcell-to-celldiffusionbetweencells.Both  proteins,butnotthecoatproteins(CP)oftherespectiveviruses,functionally trans-complement cell-to-  cell movement-defective PotatovirusX (PVX) and Tobaccomosaicvirus (TMV) mutants.The54Kand54K  proteinsinteractwiththevirus-specific CPinthecytoplasm,suggestingapotentialroleofCPin  ophiovirus movement.Thisisthe first studycharacterizingthemovementproteins(MP)ofophioviruses","palabras_clave":"Citrus psorosis virus |Mirafiori lettuce big-vein virus|ophiovirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63661,"idioma_id":4,"titulo":"Dynamical analysis of the Gliese-876 Laplace resonance","resumen":"The number of multiple-planet systems known to be involved in meanmotion conmensurabilities has increased significantly since the Keplermission. Although most correspond to two-planet resonances, multipleresonances have also been found. The Laplace resonance is a particularcase of a three-body resonance in which the period ratio betweenconsecutive pairs is n1\/n2 ?n2\/n3 ? 2\/1. It is not clear how this tripleresonance acts to stabilize (or not) the system.The most reliable extrasolar system located in a Laplace resonance is GJ876, because it has two independent confirmations. However, best-fitparameters were obtained without previous knowledge of resonancestructure, and not all possible stable solutions for the system havebeen explored.In the present work we explore the various configurations allowed by theLaplace resonance in the GJ 876 system by varying the planetaryparameters of the third outer planet. We find that in this case theLaplace resonance is a stabilization mechanism in itself, defined by atiny island of regular motion surrounded by (unstable) highly chaoticorbits. Low-eccentricity orbits and mutual inclinations from -20° to20° are compatible with observations. A definite range of mass ratiomust be assumed to maintain orbital stability. Finally, we provideconstraints on the argument of pericentres and mean anomalies to ensurestability for this kind of system.","palabras_clave":"planetas extrasolares|resonancias|estabilidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63662,"idioma_id":4,"titulo":"Seed production in Polylepis australis (Rosaceae) as influenced by tree size, livestock and interannual climate variations in the mountains of central Argentina","resumen":"The expansion of tree species within a grassland-woodland matrix relies heavily on an abundant seed crop, which may be affected by many factors; among these, browsing by livestock has gone largely ignored. Here we explore how seed production is affected by tree size, grazing by large domestic herbivores and interannual climate fluc-tuations in a small-sized tree (Polylepis australis, Rosaceae), which dominates the remnant forests in the high mountains of central Argentina. Seed number and seed mass per tree  were quantified in 100 different-sized trees located in areas with and without livestock. Using seed traps we also determined seed rain variation in 15 plots between 2005 and 2008. The estimated number of seeds per tree ranged from 0 to 224,000 and increased with tree height (power relation); for trees smaller than 354 cm in height, seed production was lower in areas with livestock and higher in areas without livestock. Mass per seed increased  with tree height, with no significant livestock effect. The largest seed rain was recorded in 2008, probably due to growth of seed trees and not in response to climate. To promote seed production and forest expansion, livestock should be excluded from areas with small trees, such as forest edges.","palabras_clave":"MOUNTAIN FOREST|LARGE HERBIVORES|SEED MASS|BROWSING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63663,"idioma_id":4,"titulo":"Occurrence of top carnivores in relation to land protection status, human settlements and rock outcrops in the high mountains of central Argentina","resumen":"We evaluated puma (Puma concolor) and culpeo fox (Pseudalopex culpaeus smithersi) occurrence in relation to a national park where livestock was reduced and hunting banned, and in relation to human presence and landscape features. In the high mountains of central Argentina puma signs were about 3.5 times less frequent than culpeo signs. Puma and culpeo signs were 33 and 19 times, respectively, more abundant within than outside the park. Puma signs increased with distance to settlements within the park but no trend was detected outside the park where signs were scarce. Culpeos were abundant all throughout the park with no relation with distance to settlements while outside the park signs increased with distance from settlements. Rock outcrops had a positive effect on pumas, which used this habitat frequently, and negative effect on culpeos, whose presence decreased slightly at sites with the highest proportion of rock outcrops inside the park and sharply at sites with presence of rock outcrops outside the park. We conclude that top carnivores were positively affected by the park creation and encourage the creation of more protected areas with connectivity in the region to better assure the long-term conservation of top predators.","palabras_clave":"Culpeo fox|generalized linear models|land use|poaching"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63664,"idioma_id":5,"titulo":"Distribución y estado de conservación de las poblaciones de árboles y arbustos del género Polylepis (Rosaceae) en las montañas de Argentina","resumen":"RESUMEN. El género Polylepis es endémico de las montañas de Sudamérica. Sus especies frecuentemente dominan   la canopia de bosques y arbustales cuya conservación y restauración son una prioridad a nivel continental. En   Argentina, el género incluye cinco especies que se distribuyen en las montañas del centro y noroeste del país.   Sus distribuciones, amenazas y estados de conservación están poco documentados e incluso existen fuertes   controversias sobre el grado en que el Hombre ha impactado en la cobertura, la estructura y la distribución   de estos bosques. Para contribuir a revertir esta situación confeccionamos modelos potenciales por especie   que sugieren 14800 km2   aptos para la presencia de al menos una de las cinco especies. Evaluamos 490 parcelas   de 3600 m2   distribuidas en 43 localidades y para cada especie describimos los bosques en mejor estado de   conservación que de manera tentativa podrían usarse como referencia para determinar metas de conservación   y en trabajos de restauración. En tres de las cinco especies encontramos evidencias de fuego en más de 60% de   las parcelas visitadas. Muchas localidades presentaban suelos con evidencias de erosión y escasa cobertura de   vegetación, así como una baja cobertura y complejidad estructural de bosque. Consideramos que Argentina   ofrece buenas oportunidades para la conservación y restauración de estos bosques ya que aún quedan grandes   extensiones, numerosos relictos, y en muchas áreas la presión antrópica ha disminuido. Es prioritario reducir   los fuegos, establecer más y mejores áreas protegidas y, en particular en el noroeste Argentino, tener un mejor   conocimiento de la ecología de cada especie y desarrollar métodos para restaurar sus bosques.","palabras_clave":"modelos de distribucion|Polylepis|Maxent"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63666,"idioma_id":4,"titulo":"Demography and socioeconomic vulnerability influence fire occurrence in Bariloche (Argentina)","resumen":"Despite the enormous impact of fires on human welfare, few studies evaluated the influence of demographic and socioeconomic conditions on the occurrence of vegetation fires. We used quantile regression to estimate the dependence of fire density (no. ha&#8722;1) on demographic and socioeconomic conditions for neighborhoods with contrasting fire density levels (i.e., quartiles), using data of Bariloche, a city with a large wildland?urban interface zone, as a case study. We evidenced that socioeconomic and demographic variables can explain part of the fire density?s variability not explained by biophysical variables, througha principal component analysis, and examined the goodness of fit of the model to the data through the Akaike?s Information Criterion. Opposite to that observed in natural areas, fire density increased in the recent years in the interface areas. Population density was positively and significantly related to interface fire density, likely representing the effect of anthropic pressure on the environment. Fire density increased with socioeconomic vulnerability, particularly, high unemployment rate, high level of teenagers that neither study nor work, and low educational levels. Therefore, population welfare and education must be the most important target for public policies, even from a fire preventive viewpoint. The demographic and socioeconomic influence on wildland?urban interface fires should be explicitly considered by policymakers, and complement biophysical data to plan land use and regulate forest management, as well as to measure the additional effort needed for firefighting (e.g., investments in infrastructure and extra workers) to attend the greater population.","palabras_clave":"FIRE|PATAGONIA|POPULATION DENSITY|QUANTILE REGRESSION|SOCIOECONOMIC VULNERABILITY|WILDLAND-URBAN INTERFACE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63667,"idioma_id":4,"titulo":"INFLUENCE OF PHYSICAL AND CHEMICAL SOIL PROPERTIES ON THE ADSORPTION OF Escherichia coli IN MOLLISOLS AND ALFISOLS OF ARGENTINA","resumen":"Bacterial adsorption on soils and sediments is one of the main factors that control bacte-rial transport to water bodies. In this work, 32 soil samples representative of the most im-portant arable areas of the Rolling Pampa region (Argiudolls) and bottomlands devoted to livestock production (Natraqualfs) were analyzed in order to evaluate bacterial-soil adsorption. The first axis of a principal component analysis explained 45% of the total variation among soils in 11 physical and chemical properties, and was strongly and positively related to bacterial adsorption (r =0.67). Soil bacterial adsorption presented a large range of values (25-73%), being those for Argiudolls significantly higher than those for Natraqualfs. For both soils, cation exchange capacity (CEC) (r =0.67) and clay content (r =0.55) were positively associated with bacterial adsorption; whereas exchangeable sodium percentage (ESP) showed a negative tendency (r =0.42). It is concluded that in the basin studied, granulometry, CEC and ESP proved to be impor-tant properties to discriminate bacterial-soil adsorption, and the following equation to estimate mean soil bacterial adsorption in these soils is proposed: y=1.73×CEC ?1 2? 0.05×sand(50-250 &#956;m)[g kg ]?0.54×ESP (R adjust=0.77). These results would help to monitor water quality of surface water bodies by the development of bacterial transport models using standard soil data.","palabras_clave":"contaminación hídrica|pastoreo|adsorción bacteriana|Argiudoles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63668,"idioma_id":4,"titulo":"A global quantitative synthesis of local and landscape effects on wild bee pollinators in agroecosystems","resumen":"Bees provide essential pollination services that are potentially affected both by local farm management and the surrounding landscape. To better understand these different factors, we modelled the relative effects of landscape composition (nesting and floral resources within foraging distances), landscape configuration (patch shape, interpatch connectivity and habitat aggregation) and farm management (organic vs. conventional and local-scale field diversity), and their interactions, on wild bee abundance and richness for 39 crop systems globally. Bee abundance and richness were higher in diversified and organic fields and in landscapes comprising more high-quality habitats; bee richness on conventional fields with low diversity benefited most from high-quality surrounding land cover. Landscape configuration effects were weak. Bee responses varied slightly by biome. Our synthesis reveals that pollinator persistence will depend on both the maintenance of high-quality habitats around farms and on local management practices that may offset impacts of intensive monoculture agriculture.","palabras_clave":"Agri-environment schemes|diversified farming system|ecologically scaled landscape index|ecosystem services|farm management|habitat fragmentation|landscape structure|organic farming|pollinators"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63669,"idioma_id":4,"titulo":"Adsorption and affinity of Escherichia coli   to different aggregate sizes of a silty clay soil","resumen":"The sediments produced by water erosion are the main source of pollution of agricultural origin of surface water bodies. These sediments may be associated to bacteria, compromising the quality of nearby water bodies.Therefore, to understand this biological contamination, it is necessary to find out the adsorption capacity and bacterial affinity to aggregate sizes that may result in a differential sedimentation. To this end, in the present work, the distribution, adsorption capacity and affinity to different aggregate sizes of two strains of Escherichia coli in two liquid media of contrasting ionic strength were evaluated in a silty clay soil. The &lt;2 &#956;m fraction showed a higher proportion of bacteria than the other aggregate sizes (48%), whereas among the fractions &gt;2 &#956;m, the 20&#150;50 &#956;m fraction was the one that showed the highest bacterial adsorption in both liquid media (37.9%). On the other hand, the highest values of bacterial affinity were found in the 20 to 50 &#57884;m fraction (coarse silt) in the low ionic strength media and 20&#150;50 and &gt;50 &#57884;m in the high ionic strength media. However, the bacterial strains used revealed only some trends in the modification of these variables. This work contributes to the development and implementation of strategies to mitigate pollution, such as control of sediment generation and its subsequent capture in filter strips.&nbsp;","palabras_clave":"Biological contamination|Surface transport|Water quality"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63670,"idioma_id":4,"titulo":"Substrate effect on the swelling and water sorption of Nafion nanomembranes","resumen":"Swelling and sorption of water, from the vapour phase, by thin (100?1000&nbsp;nm) and ultra-thin (&lt;100&nbsp;nm) Nafion nanomembranes deposited on several surfaces were determined using quartz crystal micro-balance and environmental ellipsometric porosimetry techniques. The water uptake at room temperature, measured as the number of water molecules per ionic group in the polymer, is much lower than that observed in bulky Nafion 117 membranes. Water sorption by supported nanofilms was found to be independent of film thickness, but it depends on substrate nature. Aging due to slow relaxation of the polymer microstructure is also discussed. Results are relevant for modelling the three-phase region of proton exchange membrane fuel cells, where a thin Nafion film covers the carbon supported catalyst nanoparticles.","palabras_clave":"Nafion|Membrane |Water|Nanofilms"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63671,"idioma_id":5,"titulo":"RESULTADOS PRELIMINARES DEL EFECTO DE LA GANADERÍA EN LA PRODUCTIVIDAD DEL ESTRATO HERBÁCEO EN EL PARQUE PROVINCIAL POTRERO DE YALA","resumen":"Se determinó la productividad del estrato herbáceo en la franja altitudinal de los 2000 msm en el Parque Provincial Potrero de Yala. Para ello se tomaron muestras durante un año en cada estación climática El criterio para ubicar las mismas, se basó en identificar subzonas con estratificación diferente de la vegetación: pastizal, arbustal y bosque.Se tomaron 19 muestras de 1m x 1m, en las que se realizó el corte del material herbáceo al ras, tres de ellas en sectores cerrados al acceso del ganado. Luego fueron secadas en estufa por 72 horas a 70 ºC y se obtuvo el peso seco de las mismas.Los resultados de la productividad del estrato herbáceo, a lo largo de un año revelaron que no hay diferencias significativas entre las muestras tomadas en áreas cerradas y las tomadas en lugares abiertos al acceso del ganado. Asimismo también se demostró que entre las estaciones no hay diferencias significativas de biomasa producida, salvo en verano que es cuando se encontró la mayor productividad del estrato herbáceo. Estos resultados preliminares indican que la carga animal actual del Parque Provincial Potrero de Yala es adecuada para el área protegida. Se necesitan resultados de varios años para dar una confirmación definitiva al respecto.","palabras_clave":"yungas|productividad herbacea|ganado"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63672,"idioma_id":4,"titulo":"Boron Neutron Capture Therapy for oral pre-cancer: proof of principle in an experimental animal model.","resumen":"Objectives: Field cancerized tissue can give rise to second primary tumours, causing therapeutic failure. Boron Neutron Capture Therapy (BNCT) is based on biological targeting and would serve to treat undetectable foci of malignant transformation. The aim of this study was to optimize BNCT for the integral treatment of oral cancer, with particular emphasis on the inhibitory effect on tumour development originating in pre-cancerous conditions, and radiotoxicity of different BNCT protocols in a hamster cheek pouch oral pre-cancer model. Materials and Methods: Groups of cancerized hamsters were locally exposed to single or double (2 or 4 weeks apart) applications of BNCT at different dose levels, mediated by the boron compounds boronophenylalanine (BPA) or BPA and decahydrodecaborate (GB-10) administered jointly. Cancerized, sham-irradiated hamsters served as controls. Clinical status, tumour development from field cancerized tissue and mucositis were followed for 8 months. Results: A double application (4 weeks apart) of BNCT mediated by GB-10 + BPA at a total dose of 10 Gy in two 5 Gy doses rendered the best therapeutic advantage (63% - 100% inhibition of tumour development from field cancerized tissue), minimizing dose-limiting mucositis. Conclusion: BNCT can be optimized for the integral treatment of head and neck cancer, considering the implications for field cancerized tissue.","palabras_clave":"Boron Neutron Capture Therapy|BNCT|hamster cheek pouch oral pre-cancer model|field cancerization|pre-cancerous conditions|oral cancer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63673,"idioma_id":4,"titulo":"Polyethylene glycol-based low generation dendrimers functionalized with &#946;-cyclodextrin as cryo- and dehydro-protectant of catalase formulations.","resumen":"Polyethylene glycol (PEG)-based low generation dendrimers are analyzed as single excipient or combined with trehalose in relation to their structure and efficiency as enzyme stabilizers during freeze-thawing, freeze-drying, and thermal treatment. A novel functional dendrimer (DGo-CD) based on the known PEG´s ability as cryo-protector and B-CD as supramolecular stabilizing agent is presented. During freeze-thawing, PEG and B-CD failed to prevent catalase denaturation, while dendrimers, and especially DGo-CD, offered the better protection to the enzyme. During freeze-drying, trehalose was the best protective additive but DGo-CD provided also an adequate catalase stability showing a synergistic behavior in comparison to the activities recovered employing PEG or B-CD as unique additives. Although all the studied dendrimers improved the enzyme remaining activity during thermal treatment of freeze-dried formulations, the presence of amorphous trehalose was critical to enhance enzyme stability. The crystallinity of the protective matrix, either of PEG derivatives or of trehalose, negatively affected catalase stability in the freeze-dried systems. When humidified at 52% of relative humidity, the dendrimers delayed trehalose crystallization in the combined matrices, allowing extending the protection at those conditions in which normally trehalose fails. The results show how a relatively simple covalent combination of a polymer such as PEG with B-CD could significantly affect the properties of the individual components. Also, the results provide further insights about the role played by polymer-enzyme supramolecular interactions (host - guest crosslink, hydrogen bonding, and hydrophobic interactions) on enzyme stability in dehydrated models, being the effect on the stabilization also influenced by the physical state of the matrix.","palabras_clave":"dendrimer|enzyme stability|b-cyclodextrin|dehydration|freezing and thawing|PEG|trehalose|catalase"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63674,"idioma_id":4,"titulo":"First evidence of sucrose biosynthesis by single cyanobacterial bimodular proteins","resumen":"The net synthesis of  sucrose (Suc) is catalysed by the sequential  action of Suc-phosphate synthase (SPS) and Suc-phosphate phosphatase (SPP). SPS  and SPP from Anabaena sp. PCC 7120 (7120-SPS and 7120-SPP) define minimal catalytic units. Bidomainal SPSs,  where both units are fused, occur in plants and cyanobacteria, but they display  only SPS activity. Using recombinant proteins that have fused 7120-SPS and  7120-SPP, we demonstrated that they are bifunctional chimeras and that the arrangement  7120-SPS\/SPP is the most efficient to catalyse the sequential reactions to  yield Suc. Moreover, we present the first evidence of a bidomainal SPS present  in the cyanobacterium Synechococcus  elongatus PCC 7942 with both, SPS and SPP activity.","palabras_clave":"SUCROSE-PHOSPHATE SYNTHASE|BIFUNCTIONAL|CYANOBACTERIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63675,"idioma_id":4,"titulo":"PSG gene expression is up-regulated by lysine acetylation involving histone and nonhistone proteins","resumen":"Background: Lysine acetylation is an important post-translational modification that plays a central role in eukaryotic  transcriptional activation by modifying chromatin and transcription-related factors. Human pregnancy-specific glycoproteins  (PSG) are the major secreted placental proteins expressed by the syncytiotrophoblast at the end of pregnancy and  represent early markers of cytotrophoblast differentiation. Low PSG levels are associated with complicated pregnancies,  thus highlighting the importance of studying the mechanisms that control their expression. Despite several transcription  factors having been implicated as key regulators of PSG gene family expression; the role of protein acetylation has not been  explored.  Methodology\/Principal Findings: Here, we explored the role of acetylation on PSG gene expression in the human  placental-derived JEG-3 cell line. Pharmacological inhibition of histone deacetylases (HDACs) up-regulated PSG protein and  mRNA expression levels, and augmented the amount of acetylated histone H3 associated with PSG 59regulatory regions.  Moreover, PSG5 promoter activation mediated by Sp1 and KLF6, via the core promoter element motif (CPE, 2147\/2140),  was markedly enhanced in the presence of the HDAC inhibitor trichostatin A (TSA). This effect correlated with an increase in  Sp1 acetylation and KLF6 nuclear localization as revealed by immunoprecipitation and subcellular fractionation assays. The  co-activators PCAF, p300, and CBP enhanced Sp1-dependent PSG5 promoter activation through their histone acetylase  (HAT) function. Instead, p300 and CBP acetyltransferase domain was dispensable for sustaining co-activation of PSG5  promoter by KLF6.  Conclusions\/Significance: Results are consistent with a regulatory role of lysine acetylation on PSG expression through a  relaxed chromatin state and an increase in the transcriptional activity of Sp1 and KLF6 following an augmented Sp1  acetylation and KLF6 nuclear localization.","palabras_clave":"PLACENTA|GENE REGULATION|PREGNANCY|SP\/KLF FAMILY|CO-ACTIVATORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63676,"idioma_id":5,"titulo":"'Identificaciones étnico nacionales y procesos de legitimación del saber en grupos indígenas y migrantes en Argentina'","resumen":"En este trabajo abordamos la relación entre saberese identificaciones en grupos indígenas y migrantes y los procesosde legitimación de los saberes en espacios familiares y escolares.En un breve recorrido inicial identificamos antecedentes en el abordajede estas cuestiones desde la antropología clásica, los aportes delas perspectivas crítico reproductivistas y de trabajos antropológicossobre aprendizaje y prácticas sociales. En la segunda parte aludimosa situaciones registradas en el trabajo con dos poblaciones (comunidadesmbyà guaraníes de Misiones, inmigrantes bolivianos en laProvincia de Buenos Aires), las que nos permiten realizar reflexionessituadas y avanzar en algunas comparaciones significativas. Consideramosque abordar conjuntamente la cuestión de los saberes, lasidentificaciones y los procesos de legitimación aporta elementospara la definición de propuestas educativas adecuadas a los complejoscontextos interculturales que caracterizan la situación de losniños indígenas y migrantes en Argentina","palabras_clave":"SABERES|INDIGENAS|MIGRANTES|INTERCULTURALIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63677,"idioma_id":5,"titulo":"Reproductive pattern in the southernmost populations of South American Redbelly Toads","resumen":"Anurans can be classified as explosive or prolonged breeders. In species with short breeding periods, intensive competition for females is expected. We analyze the temporal breeding pattern of Melanophryniscus aff. montevidensis, a bufonid inhabiting grasslands of Argentina, whose breeding is concentrated in events of short duration. Males are active during more than one breeding event per season, while most females take part in a single event per season. Active males outnumbered females by up to 14 times at one event, operational sex ratio (OSR) ranged from 0.07 to 0.75 and intensity of sexual selection (Is) from 0.69 to 24. We found a negative association between Is and OSR, but no correlation between number of active males per event and OSR or Is. OSR is the key that regulates the intensity of sexual selection, nevertheless, and in spite of being explosive breeders, females would potentially have chances to select their mate. ","palabras_clave":"BIOLOGÍA REPRODUCTIVA|ANFIBIOS|SELECCIÓN SEXUAL|PASIZALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63678,"idioma_id":4,"titulo":"New family host and records of Acanthocrios furnarii (Cordero & Vogelsang, 1928) (Hemiptera: Cimicidae) from Argentina, and implications in the transmission mechanism of cimicid bugs among birds? nests","resumen":"Acanthocrios furnarii (Cordero &amp; Vogelsang, 1928) [Hemiptera: Cimicidae: Haematosiphoninae] is an ectoparasite on avian hosts from Argentina and Uruguay. It has been mostly found in mud nests of Furnarius rufus (Gmelin, 1788) [Aves: Furnariidae], but its true hosts are some of the inquiline birds that use F. rufus nests. These inquiline hosts belong to the families Emberizidae, Hirundinidae, Icteridae, Passeridae, and Troglodytidae. Outside F. rufus mud nests, A. furnarii hasbeen found in nests of other Furnariidae, Hirundinidae, and Passeridae. The present work adds the first nonpasserine host (Falconidae) of A. furnarii, together with new records in La Pampa, Argentina. The transmission mechanism of A.furnarii, together with all other cimicid bugs from Argentina and adjacent countries, is increased considering this new host; and we also take into account the birds that nidificate in nest boxes, the cavity-nesting birds in trees and earth, andthe inquiline birds in stick nests of Furnariidae and Psittacidae.","palabras_clave":"bird bugs|Hemiptera|Cimicidae|Falconidae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63679,"idioma_id":4,"titulo":"Physical properties and mechanical behaviour of concrete made with recycled aggregates and fly ash","resumen":"Enhancing the environmental sustainability of human activities and industrial processes is a common challenge in various branches of modern research and technology. Being characterised by a huge demand of both energy and raw materials and by a significant contribution to the global emissions of greenhouse gases (GHGs), the construction industry is fully concerned by this challenge. Particularly, since concrete is the most widely used construction material, several solutions are nowadays under investigation to reduce the environmental impact of its production processes. They often consist of partially replacing \u2018\u2018natural\u2019\u2019 constituents (i.e. aggregates, cement, water, fibres) with recycled ones, in view of the twofold objective of reducing both the demand of raw materials and the amount of waste to be disposed in landfills. However, the formulation of reliable relationships between the main physical and mechanical properties of concrete produced with the aforementioned recycled constituents is still considered as an open issue.This paper is a contribution to such topic. The results of a wide experimental campaign carried out on concretes made using recycled concrete aggregates (RCAs) and fly-ash (FA) in partial substitution of natural aggregates (NAs) and cement (C) are presented and discussed herein. Particularly, concretes characterised by variable water\u2013binder ratios and produced with different percentages of RCA and variable the content of FA have been tested. Test results have allowed estimating the time evolution of the compressive strength, as well as the tensile strength at 28 days, along with some relevant physical properties, such as permeability and resistance to chloride ion penetration. The feasibility of producing&nbsp; structural concrete made with even significant amounts of the aforementioned recycled constituents and industrial by-products clearly emerges from the experimental results.","palabras_clave":"Sustainability|Concrete|Recycled aggregates|Fly ash|Experimental tests"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63680,"idioma_id":4,"titulo":"A fracture-based interface model for simulating the bond behaviour of FRP strips glued to a brittle substrate","resumen":"The present paper deals with the bond behaviour of Fiber Reinforced Polymer (FRP) laminates glued to brittle substrates. Based on a similar model already available in the scientic literature for discrete-crack analyses on plain concrete, the proposed work formulates a numerical model completely conceived within the general framework of fracture mechanics and plasticity-based concepts. The most common proposals and theoretical models available in the scientic literature and adopted for describing the bond behaviour of FRP-to-brittle adhesive joints are generally based on assuming 'a priori'the analytical expression of the interface bond-slip law, while a more general formulation developed within the framework of fracture mechanics is proposed in this paper. Numerical applications demonstrate the soundness of the proposed formulation and show a very good agreement with experimental results of FRP-to-concrete bond-slip tests.","palabras_clave":"Bond, |Brittle substrate,|Fracture mechanics, |FRP,| Zero-thickness interface"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63681,"idioma_id":4,"titulo":"A numerical recipe for modelling hydration and heat flow in hardening concrete","resumen":"This paper presents a sound theoretical formulation and an effective numerical implementation of a heatflowand hydration model for concrete hardening. The model is based on the Fourier equation of heat flowwith the adiabatic hydration curve employed as a reference for simulating the hydration heat source. Theproposed formulations are based on a consistent scheme for the partial differential equation and itsboundary and starting conditions. The hydration kinetics is simulated through the Arrhenius approach.Formulations for the compressive strength and the elastic modulus are provided and the maturity functionis also considered. A finite difference numerical solution is derived with a forward explicit time integrationin the time\u2013space domain. The numerical solution is designed as a stepwise \u2018\u2018recipe\u2019\u2019 specificallyconceived to be easily implemented by means of either a high-level programming language or even aspreadsheet tool. Experimental temperature measurements for two different mixtures, under adiabaticand semi-adiabatic conditions, are used for validating the proposed model. The adiabatic and semi-adiabatictemperature simulations show good agreement with the experimental data for both concrete mixtures.The degree of hydration could be simulated and used as the fundamental parameter for scrutinisingthe evolution of the compressive strength. Particularly, a linear trend between the compressive strengthand the degree of hydration and the maturity was figured out.","palabras_clave":"Heat| Hydration |Cement |Arrhenius |Temperature| Strength"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63682,"idioma_id":4,"titulo":"Copper nanoparticles supported on silica coated maghemite as versatile, magnetically recoverable and reusable catalyst for alkyne coupling and cycloadditon reactions","resumen":"A versatile and magnetically recoverable catalyst consisting of copper nanoparticles on silica coated maghemite nanoparticles (MagSilica®) is presented. The catalyst has been prepared under mild conditions by fast reduction of anhydrous CuCl2 with lithium sand and a catalytic amount of DTBB (4,4\u2019-di-tert-butylbiphenyl) as electron carrier, in the presence of the magnetic support. The catalyst has been fully characterized and its performance in different coupling and cycloaddition reactions of terminal alkynes has been studied. This new copper-based catalyst has shown to be very efficient and easily reusable in the Glaser alkyne dimerization reaction in THF, the multicomponent Huisgen 1,3-dipolar cycloaddition reaction in water and the three-component synthesis of propargylamines under solvent free conditions.","palabras_clave":"COPPER NANOPARTICLES|MAGNETIC SUPPORT|ALKYNE|COUPLING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63683,"idioma_id":4,"titulo":"High Surface Area CeO2 Promoted Suzuki?Miyaura Cross-Coupling of Arylboronic Acids and Aryl Iodides","resumen":"Abstract: High surface area cerium oxide (240 m2\/g) efficientlypromoted the Suzuki?Miyaura cross-coupling of aryl iodides witharylboronic acids, in the absence of palladium or any othertransition-metal catalyst. Excellent selectivity towards the crosscouplingproduct was observed. Cerium oxide could be recoveredand reused at least three times without any loss of activity.","palabras_clave":"BIARYLS|CROSS COUPLING|CERIUM OXIDE|SUZUKI-MIYAURA REACTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63684,"idioma_id":4,"titulo":"Structure of neutron stars in R-squared gravity","resumen":"The effects implied for the structure of compact objects by the modification of General Relativity (GR) produced by the generalization of the Lagrangian density to the form f(R)=R+&#945;R2 , where R is the Ricci curvature scalar, have been recently explored. It seems likely that this squared-gravity may allow heavier Neutron Stars (NSs) than GR. In addition, these objects can be useful to constrain free parameters of modified-gravity theories. The differences between alternative gravity theories are enhanced in the strong gravitational regime. In this regime, because of the complexity of the field equations, perturbative methods become a good choice to treat the problem. Following previous works in the field, we performed a numerical integration of the structure equations that describe NSs in f(R) -gravity, recovering their mass-radius relations, but focusing on particular features that arise from this approach in the profiles of the NS interior. We show that these profiles run in correlation with the second-order derivative of the analytic approximation to the Equation of State (EoS), which leads to regions where the enclosed mass decreases with the radius in a counter-intuitive way. We reproduce all computations with a simple polytropic EoS to separate zeroth-order modified gravity effects.","palabras_clave":"MODIFIED GRAVITY|NEUTRON STARS|GENERALISED GRAVITY EQUATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63685,"idioma_id":4,"titulo":"Cell death mechanisms during follicular atresia in Dipetalogaster maxima, a vector of Chagas' disease (Hemiptera: Reduviidae).","resumen":"              In  this work we have analyzed the involvement of cell death pathways during the  process of follicular atresia in the hematophagous insect vector Dipetalogaster  maxima. Standardized insect rearing conditions were established to induce a  gradual follicular degeneration stage by depriving females of blood meal during  post-vitellogenesis. We first characterized the morpho-histological and  ultrastructural changes of the ovarian tissue at early and late follicular  atresia by light and transmission electron microscopy. Apoptosis was  investigated by DAPI nuclear staining, TUNEL labeling and the detection of  active caspase-3 by immunofluorescence. Autophagy was assessed by the  measurement of acid phosphatase activity in ovarian homogenates and monitored  by the detection of the specific marker of autophagic compartments, LC3. High  levels of acid phosphatase activity were detected at all atretic stages.  However, follicular cells of follicles undergoing incipient degeneration observed  in early atresia exhibited features of apoptosis such as chromatin  condensation, DNA fragmentation and the presence of active caspase-3. The  ultrastuctural findings and the increased levels of LC3-II founded at late  follicular atresia supported the relevance of autophagy at this atretic stage,  although the extent of autophagosome formation demonstrated that this cell  death pathway also occurred at early atresia. In late atresia, follicular cells  also displayed more drastic changes compatible with necrosis. Taken together, results  showed that apoptosis, autophagy and necrosis were operative during follicular  atresia in D. maxima. Moreover, it was shown that the relevance of these  cell death mechanisms correlates with the time elapsed since the onset of the  degenerative process.              Keywords:Dipetalogaster maxima, reproduction,  follicular atresia, apoptosis, autophagy, necrosis.  ","palabras_clave":"Dipetalogaster maxima|Reproduction|Follicular atresia|Apoptosis|Autophagy|Necrosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63686,"idioma_id":4,"titulo":"Chemical anchorage of polypropylene onto glass fibers. Effect on adhesion and mechanical properties of their composites","resumen":"The objective of this work is to study the influence of the polypropylene (PP) chains, chemically anchored onto glass fibers (GF), over the adhesion and consequently on mechanical properties of the PP\/GF composites. The anchoring of glass fibers to the polymer matrix was achieved by direct metallocenic polymerization of propylene onto the fibers surface, previously treated with methylaluminoxane. With this method, homogeneous fiber coverage is obtained, drastically changing the fiber surface activity, from hydrophilic to hydrophobic. Sandwich PP\/GF\/PP specimens were prepared including pre-straightened and uniaxially oriented fibers. The yield and break elongation, as well as the toughness of composites having polymerized fibers were notably improved indicating clearly a much greater PP\/GF adhesion. These results were corroborated by electronic microscopy analysis on the specimens after tensile tests and interpreted in terms of a phenomenological analysis of the interaction between polymerized fibers and polymer.","palabras_clave":"composites|adhesion by chemical bonding|in-situ polymerization|polypropylene-glass fiber composites"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63687,"idioma_id":4,"titulo":"Surface modified talc particles by acetoxy groups grafting. Effects on mechanical properties of polypropylene\/talc composites","resumen":"This paper deals with the effects of surface modified talc particles on mechanical properties of polypropylene\/talc composites. These materials were prepared by injection molding of polypropylene (PP) blended with different concentrations of non-treated and treated talc, under the same processing conditions. Differential Thermal Calorimetry (DSC) and Scanning Electron Microscopy (SEM) were used to assess thermal properties and morphology of the final composites. The reinforcing effect of talc, either treated or non-treated surface, on PP is analyzed through the tensile properties as a function of the mineral content (0-10 wt %).  Morphological structure of composites revealed that the talc treatment improved the particle dispersion and distribution within the PP matrix and enhanced the interfacial PP-talc adhesion. The mechanical properties of these composites, especially the Young modulus, tensile strength and elongation at break, were found to be improved respect to PP-untreated talc ones. Article first published online : 6 JUL 2012, DOI: 10.1002\/pen.23243","palabras_clave":"Composites|Mechanical properties|organoclays"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63688,"idioma_id":4,"titulo":"Influence of talc morphology on the mechanical properties of talc filled polypropylene","resumen":"Injected polypropylene (PP)\/talc composites were studied to evaluate the influence of different talc morphologies on their final mechanical properties. Talc genesis is responsible for type and content of associated minerals, crystalline degree, particle size distribution, and specially, morphology. Consequently, when talc is incorporated into PP, differences in the genesis mean differences in particle morphology and may influence the composite properties. Two talc samples having predominant macro and microcrystalline morphologies were used to prepare two composite sets to be tested. Thermal and mechanical properties of each PP\/talc composites set were measured, analyzed and discussed comparatively. The results reveal that the main influences of talc morphology are given on modulus, yield strength and elongation at break of PP\/talc composites. Macrocrystalline morphology of talc induces better composite mechanical properties than microcrystalline one. This behavior could be explained by talc characteristics as lamellarity, crystalline character and crystallinity degree.","palabras_clave":"MECHANICAL PROPERTIES|TALC MORPHOLOGY|POLYPROPYLENE|COMPOSITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63689,"idioma_id":4,"titulo":"Nucleation and growth of silver nanostructures onto HOPG electrodes in the presence of picolinic acid","resumen":"Silver electrodeposition onto HOPG electrodes in the presence of picolinic acid (PA) has been studied bycyclic voltammetry and chronoamperometry. Changes in the nucleation and growth mechanisms, whichare dependent on the PA concentration and solution pH, have been observed. Formation of complexeswith Ag+ ions and adsorption of the additive molecules on both, the substrate and the growing silver crystallitescan be correlated with the potential dependence of the kinetic parameters, No and A. The amount,distribution (random or localized on the defects sites) and size of the crystallites on the substrate areinfluenced by the composition of the solution (PA concentration and pH) when the control of the nucleationand growth processes is regulated by application of a double potential program.","palabras_clave":"Silver nanostructures|Nucleation and growth|Electrocrystallization|Picolinic acid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63690,"idioma_id":4,"titulo":"Chemical and Electrochemical Oxidation of Silicon Surfaces Functionalized with APTES: The Role of Surface Roughness in the AuNPs Anchoring Kinetics","resumen":"Oxidation of Si(111) surfaces is a procedure widely used for their further functionalization with 3-aminopropyltriethoxysilane (APTES). In the present work, the formation of silicon oxide is carried out by chemical and electrochemical oxidation of the hydrogenated-silicon surfaces, giving rise to Si-OxChem and Si-OxEchem surfaces, respectively. Both surfaces are then functionalized with APTES solution to form an aminopropylsilane (APS) film, using two quite different concentrations of APTES (0.001 and 0.1% v\/v), to compare two limiting situations. At the lowest APTES concentration, the comparison of the kinetics of gold nanoparticles (AuNPs) anchoring process on both surfaces is found to be quite different, not only in the initial rate of NPs anchoring but also in the maximum percentage of coverage. In contrast, the kinetics behavior is almost the same when the surfaces are modified with the highest APTES concentration, reaching the same value of surface coverage. The different or similar behavior of both surfaces is analyzed by a careful characterization of Si-OxChem and Si-OxEchem surfaces using XP spectroscopy and AFM measurements, before and after APS functionalization. The significant differences in the surface roughness of the Si-Ox samples, together with the determination of the number of &#8722;NH3+ moieties after silanization at both APTES concentrations, leads to the conclusion that the availability of &#8722;NH3+ moieties is dependent on two factors: the roughness of the Si-OxChem and Si-OxEchem surfaces as well as the concentration of the APTES solutions. When the APS layer is formed at the lowest APTES concentration, surface roughness controls the number of different types of nitrogen functional groups. In contrast, at the highest APTES concentration, the surface roughness does not have any significant role in the number of &#8722;NH3+ moieties present on both surfaces. Because the kinetics of AuNPs anchoring depends mainly on the probability of interacting with the &#8722;NH3+ groups, the above characterization allows us to explain in a consistent way the kinetics behavior observed for each particular condition of surface preparation. Si-OxChem and Si-OxEchem surfaces, respectively. Both surfaces are then functionalized with APTES solution to form an aminopropylsilane (APS) film, using two quite different concentrations of APTES (0.001 and 0.1% v\/v), to compare two limiting situations. At the lowest APTES concentration, the comparison of the kinetics of gold nanoparticles (AuNPs) anchoring process on both surfaces is found to be quite different, not only in the initial rate of NPs anchoring but also in the maximum percentage of coverage. In contrast, the kinetics behavior is almost the same when the surfaces are modified with the highest APTES concentration, reaching the same value of surface coverage. The different or similar behavior of both surfaces is analyzed by a careful characterization of Si-OxChem and Si-OxEchem surfaces using XP spectroscopy and AFM measurements, before and after APS functionalization. The significant differences in the surface roughness of the Si-Ox samples, together with the determination of the number of &#8722;NH3+ moieties after silanization at both APTES concentrations, leads to the conclusion that the availability of &#8722;NH3+ moieties is dependent on two factors: the roughness of the Si-OxChem and Si-OxEchem surfaces as well as the concentration of the APTES solutions. When the APS layer is formed at the lowest APTES concentration, surface roughness controls the number of different types of nitrogen functional groups. In contrast, at the highest APTES concentration, the surface roughness does not have any significant role in the number of &#8722;NH3+ moieties present on both surfaces. Because the kinetics of AuNPs anchoring depends mainly on the probability of interacting with the &#8722;NH3+ groups, the above characterization allows us to explain in a consistent way the kinetics behavior observed for each particular condition of surface preparation. ","palabras_clave":"SILICON SURFACE ROUGHNESS|APTES-FUNCTIONALIZATION|GOLD NANOPARTICLES ANCHORING|ANCHORING KINETICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63691,"idioma_id":5,"titulo":"Diferenciación de quesos tipo grana de distintos orígenes a través del análisis de los perfiles de compuestos volátiles","resumen":"La evaluación de los parámetros fisicoquímicos y microbiológicos y de las características sensoriales de los quesos contribuye al conocimiento del proceso de maduración, otorga valor agregado y permite encontrar marcadores que posibiliten distinguir un dado producto de otros de características similares presentes en el mercado, protegiendo la identidad de los mismos. En el presente estudio se analizó la fracción aromática de muestras comerciales de quesos tipo grana de diferentes orígenes: Reggianito (R) de Argentina y Parmigiano Reggiano (PR) y Grana Padano (GP), de distintas regiones de Italia. Se utilizó la microextracción en fase sólida (SPME) acoplada a cromatografía gaseosa con detección de ionización de llama y espectrometría de masas (GC-FID\/MS) con el fin de obtener los perfiles de compuestos volátiles y de este modo, determinar similitudes y diferencias tanto cualitativas como cuantitativas. Las principales diferencias en los perfiles de compuestos volátiles de los distintos quesos fueron de tipo cuantitativo. Los compuestos volátiles de mayor incidencia en el flavor de los quesos tipo grana presentaron mayores valores de áreas en los quesos italianos. El análisis de las proporciones en que los diferentes grupos químicos de compuestos contribuyen a los perfiles de volátiles reveló que tanto en los quesos PR y GP como en los quesos R hubo una prevalencia de compuestos del grupo de los ácidos. Sin embargo, diferencias significativas (P &#61603; 0.05) se encontraron en los grupos de las cetonas, ésteres, aldehídos y alcoholes. De este modo, el perfil de compuestos volátiles de los quesos R se caracterizó por una particular incidencia de compuestos del grupo de los alcoholes, cetonas y aldehídos mientras que en los quesos italianos se observó una mayor contribución de compuestos de los grupos de las cetonas, alcoholes y ésteres. El análisis multivariado de los datos mostró una discriminación de las muestras de quesos de acuerdo a su origen.","palabras_clave":"quesos tipo grana|microextracción en fase sólida (SPME)|compuestos volátiles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63692,"idioma_id":4,"titulo":"Can livestock and fires convert the sub-tropical mountain rangelands of central Argentina into a rocky desert?","resumen":"Soil erosion, as a result of livestock grazing, has been widely reported for arid and semi-arid ecosystems, but information is lacking in more mesic ecosystems where erosion is generally studied in relation to agriculture. To test the hypothesis that, in the high-mountain rangelands of Córdoba (Argentina), grazing by livestock can drive the system into a rocky desert, two-hundred 4 x 4 m plots under different livestock stocking rates and timings of grazing were monitored for five years. Four indicators of soil erosion: change rate of rock surface and of total bare surface, advance rate of erosion edges, and their activity persistence were estimated for each plot. Erosion edges are steps with a vertical bare soil surface, whose advance usually leaves behind an exposed rock area. For each plot, the average annual stocking rate for the five-year period, and an index of seasonality, were calculated. Multiple regressions were used to analyze the data. Under high stocking rates, rock and bare surface increased, edges advanced faster and persisted more actively, while under low or nil stocking rates, rock and bare surface decreased and edges tended to stabilize. From these results, it was estimated that under high stocking rates, 18 % of the whole area could be transformed into rocky surface in 400 years. As fire is a usual tool for this rangeland management, surface soil loss during one year in 77 burned and unburned plots, with and without post-fire livestock grazing, were compared. Burned plots lost 0.6 cm of surface soil when grazed, and 0.4 cm when ungrazed, while unburned plots lost less than 0.05 cm when grazed, and gained 0.07 cm when ungrazed. It was concluded that the present-day combination of livestock and fire management has the potential to convert this rangeland into a rocky desert. It is suggested that commercial livestock production, as it is carried-on at present, is not sustainable, and some suggestions on changes necessary for a future sustainable grazing industry are made.","palabras_clave":"desertification|land degradation|overgrazing|restoration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63693,"idioma_id":4,"titulo":"Molecular structure of 4-hidroxy-3-(3-methyl-2-butenyl) acetophenone, a plant antifungal, by X-ray diffraction, DFT calculation, and NMR and FTIR spectroscopy","resumen":"The molecular structure of two mixed and closely related conformers of the title compound, C13H16O2, found in the solid with unequal occupancies has been determined by X-ray diffraction methods. The substance crystallizes in the monoclinic Pca21 space group with a = 17.279(2), b = 5.1716(7), c = 12.549(2) Å, and Z = 4 molecules per unit cell. The structure was solved from 1314 reflections with I &gt; 2s(I) and refined to an agreement R1-factor of 0.049. The minor conformer (34.7%) is nearly mirror-related to and extensively overlapped with the major one. The skeleton of the 4-hydroxyacetophenone molecular fragment and the prenyl group, - (CH2)-(CH)=C(CH3)2, pendant arm attached to it are both planar and perpendicular to each other. A strong intermolecular O-H···O bond links neighboring molecules in the lattice to produce a polymeric structure. The conformational structures of the compound in the gas phase have been calculated by the DFT method and the geometrical results have been compared with the X-ray data. These data allow a complete assignment of vibration modes in the solid state FTIR and Raman spectra. The calculated 1H and 13C chemicals shifts are in good agreement with the corresponding experimental NMR spectra of the compound in solution. ","palabras_clave":"4-Hydroxy-3-(3-methyl-2-butenyl) acetophenone|Vibrational spectra|Molecular structure|Force field|DFT calculations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63694,"idioma_id":4,"titulo":"Ethanol steam reforming for hydrogen generation over structured catalysts","resumen":"abstract  The present paper reports on the preparation, characterization and reaction evaluation of structured catalysts toward hydrogen generation via ethanol steam reforming. To these ends, 400 cpsi cordierite monoliths were functionalized with RhePd\/CeO2 catalyst. SEM, TEM and XRD showed a uniform and well-covering CeO2 layer where RhePd nanoparticles of less than 0.5 nm were anchored. The functionalized monoliths were successfully tested for synthesis gas production from ethanol steam reforming. Realistic operating conditions were selected, including temperatures between 500 and 950 K, pressures from 1 to 6 bar, undiluted ethanol:water molar ratios 1:4e1:8 (i.e., 2  S\/C  4) and a wide range of feed loads. Appropriate activity and hydrogen selectivity were verified for the catalytic system, with ca. complete ethanol conversion at T > 700 K and a minor, or even negligible, generation of by-products (acetaldehyde, acetone, ethane, ethylene). Operating at 950 K and 1.5 bar, a H2 yield of 3.4mol hydrogen permol ethanol in feed was achieved for a liquid feed  load of 0.22 mlliq\/(mgcat min) (S\/C = 3), with 8.1% of CH4 and 8.2% of CO on dry basis. Kinetic parameters of a phenomenological set of reaction rate equations were fitted against experimental data, considering ethanol decomposition (methane formation) with subsequent methane steam reforming to both CO and CO2 and wateregas shift reaction.","palabras_clave":"Hydrogen|Ethanol|Monolith|Structured"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63695,"idioma_id":4,"titulo":"Study of the production of alkaline keratinases in submerged cultures as an alternative for solid waste treatment generated in leather technology","resumen":"        Six non-pathogenic  fungal strains isolated from alkaline soils of Buenos Aires Province, Argentina  (Acremonium murorum, Aspergillus sidowii, Cladosporium cladosporoides, Neurospora tetrasperma, Purpureocillium lilacinum (formerly Paecilomyces lilacinus) and Westerdikella dispersa) were tested for  their ability to produce keratinolytic enzymes. Strains were grown on feather meal  agar as well as in solid state and submerged cultures using a basal mineral  medium and \u201Chair waste\u201D as sole sources of carbon and nitrogen. All the tested fungi  grew on feather meal agar, but only three of them were capable of hydrolyzing  keratin, producing clear zones. Among these strains, P. lilacinum produced the highest proteolytic and keratinolytic  activity, both in solid-state and submerged fermentations. Medium composition  and culture conditions for the keratinases production by P. lilacinum were optimized. Addition of glucose (5 g\/l) and yeast  extract (2.23 g\/l) to the basal hair medium, increased keratinases production.  The optimum temperature and initial pH for the enzyme production were 28ºC and  6.0, respectively. A beneficial effect was observed when the original  concentration of four metal ions, present in the basal mineral medium, was reduced  up to 1:10. The maximum yield of the enzyme was 15.96 Uc\/ml in the  optimal hair medium; this value was about 6.5-fold higher than the yield in the  basal hair medium.  These results suggest that keratinases from P. lilacinum can be useful for biotechnological  purposes such as biodegradation (or bioconversion) of hair waste leading to a reduction  of the environmental pollution caused by leather technology with the concomitant  production of proteolytic enzymes and protein hydrolyzates.","palabras_clave":"KERATINASES|HAIR WASTE|LEATHER TECHNOLOGY|PURPUREOCILLIUM LILACINUM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63696,"idioma_id":4,"titulo":"Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876","resumen":"                Enzymatic  decomposition of gelatin layers on used X-ray films and repeated utilization of  enzyme for potential application in silver recovery were investigated using keratinolytic  serine proteases from Purpureocillium  lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was  enhanced by the increase of enzyme concentration or by the increase of the temperature  up to 60 ºC. Under the conditions of 6.9 U\/ml, 60 ºC and pH 9.0, hydrolysis of gelatin  layers and the resulting release of silver particles were achieved within 6  min. The protective effect of polyols against thermal denaturation was  investigated. The presence of glycerol and propylene glycol increases enzyme  stability. When the reusability of the enzyme for gelatin hydrolysis was tested  it could be seen that it could be effectively reused for more cycles when glycerol  was added compared to the enzyme without protective agents. The results of  these repeated treatments suggested that a continuous process of recycling  silver from used X-ray is feasible. Keeping in mind that recycle is -at the  present time- needed and imperative, it can be remark that, in this research,  three wastes were used: hair waste in order to produce serine proteases;  glycerol in order to enhance enzyme thermal stability and used X-ray films in  order to recover silver and PET films.        ","palabras_clave":"SILVER RECOVERY |SERINE PROTEASES|X-RAY FILMS|THERMOSTABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63697,"idioma_id":5,"titulo":"'Ciencia' del Derecho y normas jurídicas: problemas de jerarquía, de atribución de sentido y de referencialidad","resumen":"Esta revista se encuentra indizada en Latindex Directorio.  Distribución: Nacional e internacional.            &nbsp;          Resumen:            Los intentos por superar los embates al carácter científico del estudio  de las normas jurídicas provenientes de la concepción moderna de las ciencias colisionan  constantemente con una serie de inconvenientes que plantean, precisamente,  estos criterios positivistas. En esta ponencia se describen tres de estos  problemas, a saber: 1. la relación entre las normas jurídicas generales y las  individuales y la posibilidad de obtener una explicación en conjunto que no  subordine la relevancia de una a la otra; 2. la determinación del sentido  normativo desde las perspectivas sincrónica y diacrónica y el obstáculo del  hallazgo de una verdad absoluta e invariable; y 2. el problema de la  referencialidad normativa siempre que la norma jurídica se entienda como una  entidad biplánica compuesta por su formulación y su\/s sentido\/s.             Asimismo, como abordaje posible, pero rupturista con la concepción  positivista de las ciencias, se muestra a la hermenéutica jurídica como método  adecuado de comprensión integral y superador de los inconvenientes en  tratamiento y a las normas jurídicas como textos a ser interpretados en  distintos contextos. Como marco teórico de la hermenéutica jurídica se  seleccionaron solo dos autores que parten de distintos presupuestos pero que  arriban a ciertas conclusiones semejantes: Lenio Streck y Mauricio Beuchot.          &nbsp;","palabras_clave":"normas jurídicas|cientificidad|jerarquía|hermenéutica|sentido"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63698,"idioma_id":5,"titulo":"Discurso jurídico y relaciones entre alteridades. Debates en torno a la construcción de la ciudadanía a partir de la participación política","resumen":"Esta revista se encuentra indexada en Publindex, categoría CIRC: C e indizada  en Dialnet, V-lex, Elektronische Zeitschriftenbibliotek, Lex Base, CLASE (Citas  latinoamericanas en Ciencias Sociales y Humanidades), Leyex.info y Actualidad  Iberoamericana.                    Actualmente, en el Congreso de la  Nación Argentina, se encuentra en discusión un proyecto de ley tendiente a  incorporar en el electorado a los extranjeros que hayan residido en el  territorio estatal durante al menos dos años. En este trabajo se parte de este proyecto como disparador  para efectuar una serie de reflexiones de corte teórico respecto de la  complejidad cultural de las sociedades y los desafíos que se plantean a la  democracia como sistema político y de organización de la comunidad. En  particular, se pretende repensar cómo la presencia de los extranjeros acciona  mecanismos sociales de inclusión y de exclusión que son acompañados y  respaldados por el discurso jurídico. Junto con ello, la noción de ciudadanía  se encuentra presente y enfrenta procesos de retracción o de expansión. El  objeto de este trabajo, entonces, no es llevar a cabo un análisis del proyecto  de ley sino más bien aprovechar la circunstancia de su presentación para  plantear un debate respecto de cómo se dibujan categorías sociales entre los  que son considerados parte de una sociedad y los que son mantenidos fuera de  ella.","palabras_clave":"cambios normativos|ciudadanía|alteridad|discurso jurídico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63699,"idioma_id":5,"titulo":"La transformación de la virtud moral en el pensamiento de Juan Duns Escoto","resumen":"En  su comentario a la distinción 33 del Tercer Libro de las Sentencias, Juan Duns Escoto desarrolla su doctrina referida al sujeto de las virtudes morales mediante la cual establece la voluntad como única sede posible de las mismas. En el presente trabajo se intentará mostrar que esta determinación, por un lado, es consecuencia de la previa postulación de la voluntad como única potencia moral del hombre y que, por otro, implica un fuerte debilitamiento del rol e importancia de la virtud para la perfección moral. La revista se encuentra indizada en ISI Thomson Reuters\/Web of Science, SCIELO, CCN\/Ibict, Bibliografia Teológica Comentada; Bibliographie Latinoamericaine D´Articles; Clase-Cich-Unam; International Directory of Philosophers Index; International Philosophical Bibliography; Revista Interamericana de Bibliographia; MLA International Bibliography; Dare Databank; EBSCO","palabras_clave":"JUAN DUNS ESCOTO|VIRTUD MORAL (SUJETO)|VOLUNTAD|FILOSOFIA MORAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63700,"idioma_id":5,"titulo":"Aportes para la degradación biológica de residuos sólidos de curtiembre","resumen":"El proceso de depilado conservador del pelo tienecomo beneficio hacia el medio ambiente una disminución de la contaminación enel efluente líquido de ribera, pero como contraparte, durante esteprocedimiento se genera un nuevo residuo sólido llamado \u201Cresiduo pelo\u201D que debeser apropiadamente dispuesto. En un depilado conservador del pelo se produceaproximadamente el 10% de residuo pelo en estado húmedo (humedad 70-75%)respecto al peso de piel vacuna salada. En la República Argentina, másespecíficamente en la Cuenca hídrica Matanza- Riachuelo (Provincia de BuenosAires) existen alrededor de 247 curtiembres que procesan cada una, más de milpieles diariamente. Se estima que en el país se generan más de 100 toneladasdiarias de este residuo, dando una idea de la magnitud del problema ambientalque se ha ido generando a lo largo de los años. Si bien no es considerado unresiduo peligroso por la legislación argentina (Ley 24.051) el problemaambiental que provoca es debido al gran volumen con que se genera diariamente ysu alta resistencia a la degradación. Dado que este residuo es de naturalezaproteica puede resultar interesante su utilización como materia prima paradiferentes propósitos, dando como resultado una valorización del mismo.El residuo pelo presenta como mayorcomponente orgánico (95%) una proteína llamada queratina y en menor proporciónproteínas no-queratina que son fácilmente extraídas con soluciones buffers . La queratina es la proteína más abundantepresente en las células epiteliales de vertebrados y representa el mayorconstituyente de la piel y sus apéndices: uñas, pelo, plumas y lana. Estaproteína filamentosa se encuentra empaquetada en forma de &#945;-hélices y hojas-&#946;-plegadas, unidas por puentes disulfuro, interacciones hidrofóbicas y puentes dehidrógeno que la estabilizan haciéndola insoluble en agua y resistente a ladegradación por enzimas proteolíticas como la tripsina, la pepsina y papaína .&nbsp;Existe en la naturaleza un grupode proteasas que poseen la capacidad de degradar residuos queratínicos demanera más eficiente que otras proteasas y se las conoce con el nombre dequeratinasas. Estas proteasas son producidas por varios microorganismos entrelos cuales se encuentran hongos dermatofitos de género Microsporum y Trichophyton así como también producidas por bacteriasdel género Bacillus&nbsp; y Streptomyces. En la actualidad existen escasos reportesrelacionados a la caracterización de queratinasas producidas por hongos nodermatofitos.&nbsp;La biotransformación fúngica delresiduo pelo implica considerarlo, en lugar de un residuo, una materia prima(sustrato) sobre el que el hongo actúa dando lugar a un residuo orgánicomodificado que puede ser utilizado como fuente proteica para preparadosnutricionales y en la industria de los fertilizantes. Al mismo tiempo, segenera un extracto enzimático con actividad proteolítica de interés biotecnológicocon un amplio campo de aplicación como la industria de los cosméticos,detergente, etc.&nbsp;En este trabajo se presenta la caracterizaciónbioquímica del extracto enzimático producido por Paecilomyces lilacinus (Thom) SamsonLPS#876, una cepa fúngica aislada del suelo del bosque de La Plata, cuandocrece en medio mineral mínimo en presencia de residuo pelo como única fuente decarbono y nitrógeno.","palabras_clave":"RESIDUOS SOLIDOS|QUERATINASAS|CURTIEMBRES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63701,"idioma_id":4,"titulo":"Optimization of keratinolytic protease production by Purpureocillium lilacinum LPS # 876 as a sustainable management of tannery hair waste","resumen":"Hair   waste is recognized as a solid waste generated after a hair-saving    unhairing process in leather technology and it is produced in large   amounts in  Buenos Aires province, Argentine. At the present time, the   unique option for  this solid waste is its disposal, which results in an   environmental troublesome  and a lost of money for the tanneries   because of the cost of it disposal.  Biodegradation of hair waste by   keratinolytic microorganisms represents an  alternative for   bioconversion of keratin waste. This paper reports the  optimization of   keratinases production by Purpureocillium  lilacinum LPS   # 876. Firstly, one factor-at-a-time method was used to investigate    the effect of nitrogen-sources, metal ions concentration in the basal   mineral  medium, as well as, initial pH and temperature on enzyme   production. Medium  composition for the keratinases production by P. lilacinum was   optimized using two statistical methods:  Plackett-Burman design and   central composite design -combined with response  surface methodology-   used to optimize three significant variables: initial pH,  glucose and   CaCl2. The optimal conditions were determined as 7.10 g l-1  of glucose; 0.0065 mg l-1 of CaCl2 and initial pH of  5.60; at this condition the maximum keratinase yield predicted was 26.7 U ml-1.    The model validation showed that it described adequately the influence   of  glucose and calcium concentration and initial pH on enzyme   production.  Production of keratinases by P. lilacinum  is a less-exploited field that might represent a novel and promising  biotechnological application for this microorganism.","palabras_clave":"STATISTICAL DESIGN|KERATINASE PRODUCTION |HAIR WASTE|CENTRAL COMPOSITE DESIGN|RESPONSE SURFACE METHODOLOGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63702,"idioma_id":4,"titulo":"Chemical constituents and biological activity of Euglena gracilis extracts","resumen":"Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements,suggesting the existence of diverse physiological patterns. The aim of this work is to carryout a study about secondary metabolites biosynthesis on two strains of E. gracilis culturedEuglena gracilis is a microalgae with a wide range of nutritional requirements,suggesting the existence of diverse physiological patterns. The aim of this work is to carryout a study about secondary metabolites biosynthesis on two strains of E. gracilis culturedE. gracilis culturedin vitro..Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolatedfrom an urban polluted river (MAT) were screened for preliminary identification ofchemical constituents. Both strains were studied in their photosynthetic and bleachedforms, on their exponential and stationary growth phases. Chromatographic analysis ofpigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition,and toxic activity were tested in vitro.Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolatedfrom an urban polluted river (MAT) were screened for preliminary identification ofchemical constituents. Both strains were studied in their photosynthetic and bleachedforms, on their exponential and stationary growth phases. Chromatographic analysis ofpigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition,and toxic activity were tested in vitro.in vitro.Results: The phytochemical analysis of extracts indicated the presence of steroids in allsamples, cardenolids and triterpenes in the exponential growth phase. With the exceptionof the photosynthetic MAT strain, tannins were present in all the other on exponentialphase samples and flavonoids were only observed in the stationary phase of both photosyntheticstrains. Chromatographic profiles show that chlorophyll content decreased whilecarotenoids content increased in the stationary phase of both photosynthetic strains, andreveal the presence of flavonols derived from quercetin. In concordance with the presenceof polyphenols, the fractions with the highest polarity showed antioxidant activity againstDPPH and growth inhibition activity in vitro even in the absence of paramylon, previouslyreported to have antitumoral properties.The phytochemical analysis of extracts indicated the presence of steroids in allsamples, cardenolids and triterpenes in the exponential growth phase. With the exceptionof the photosynthetic MAT strain, tannins were present in all the other on exponentialphase samples and flavonoids were only observed in the stationary phase of both photosyntheticstrains. Chromatographic profiles show that chlorophyll content decreased whilecarotenoids content increased in the stationary phase of both photosynthetic strains, andreveal the presence of flavonols derived from quercetin. In concordance with the presenceof polyphenols, the fractions with the highest polarity showed antioxidant activity againstDPPH and growth inhibition activity in vitro even in the absence of paramylon, previouslyreported to have antitumoral properties. and growth inhibition activity in vitro even in the absence of paramylon, previouslyreported to have antitumoral properties.Conclusion: This work constitutes the first report about polyphenol production in Euglenoids,which allows us a first assessment of the potential of E. gracilis as a source ofbioactive products.This work constitutes the first report about polyphenol production in Euglenoids,which allows us a first assessment of the potential of E. gracilis as a source ofbioactive products.E. gracilis as a source ofbioactive products.","palabras_clave":"Antioxidant activity|Euglena gracilis|Growth inhibition activity|Phytochemical screening polyphenols"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63703,"idioma_id":4,"titulo":"The first fossil species of Salminus, a conspicuous South American freshwater predatory fish (Teleostei, Characiformes), found in the Miocene of Argentina","resumen":"ABSTRACT?The first fossil specimen of the characiform genus Salminus is described here. The material, a threedimensionallyarticulated head, is very well preserved and allowed us to identify a new species. We could study both outer andinner bones. The specimen was found in the locality Toma Vieja, northeast of the city of Paran´ a, central eastern Argentina.The bearing horizon is the so-called ?Conglomerado os´&#305;fero,? which is putatively included in the lowermost beds of the fluvialItuzaing ´o Formation. The ?Conglomerado os´&#305;fero? is early Tortonian (early late Miocene) in age. Recent species of Salminusare top-ranking pelagic predatory fishes in temperate to tropical lowland freshwaters of South America. The aquatic vertebratefauna occurring in the bearing bed shows a similar composition to several northern South American units such as theUrumaco Formation (Venezuela, late Miocene) and La Venta Formation (Colombia, middle Miocene).","palabras_clave":"SALMINUS|MIOCENO DE PARANA|BIOGEOGRAFIA|SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63704,"idioma_id":4,"titulo":"Evaluation of the antidiarrheal activity of Rhizophora mangle L. (Rhizophoraceae)","resumen":"The bark of Rhizophora mangle L. (Rhizophoraceae), ?red mangrove?, has been used traditionally in folk medicine of Caribbean countries due to its antiseptic, astringent, antifungal and antidyspeptic properties. Owed to its astringent properties is used to treat diarrhea; however, there is no scientific evidence to support the use. This work was designed to determine the antidiarrheal effects of the bark of R. mangle in mice. The liophilized extract was given by oral gavage (125, 250, and 500 mg\/kg of weight). Pretreatment with the extract (500 mg\/kg) resulted in a significant delay of gastrointestinal motility (p <0.001). Propranolol (2.5 mg\/kg) and verapamil (5.0 mg\/kg) antagonized significantly (p <0.001) the inhibitory effect of the gastrointestinal motility caused by Rhizophora mangle L. The extract showed dose-dependent inhibitory respons in the range 125-500 mg\/kg in castor oil-induced diarrhea. In conclusion, the present results suggest that the bark of Rhizophora mangle L. produces an inhibitory effect on intestinal function, and we suppose that their action is mediated, at least in part, through &#946;-adrenergic and calcium systems.","palabras_clave":"RHIZOPHORA MANGLE L|ANTIDIARRHEAL ACTIVITY|GASTROINTESTINAL MOTILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63705,"idioma_id":4,"titulo":"White croaker (Micropogonias furnieri) paleodistribution in the Southwestern Atlantic Ocean. An archaeological perspective","resumen":"This paper presents strong and well dated evidence of the presence of Micropogoniasfurnieri in areas that are currently out of distribution. This evidence comes fromhunter-gatherer archaeological sites along the north Patagonian coast (Argentina). Ourresults allow us to determine a change in the spatial distribution of M. furnieri duringthe Holocene, which are possibly linked to biogeographic changes in a set of speciesat regional level. Finally we discuss the potential for archaeological evidence in theservice of spatial and biogeographic studies of different species.","palabras_clave":"WHITE CROAKER (Micropogonias furnieri)|PALEODISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63706,"idioma_id":4,"titulo":"Where is historical biogeography going? The evolution of the discipline in the first decade of the 21st century","resumen":"Historical biogeography studies how those processes that occur over long periods of time influence distributional patterns of life forms. It has been argued that historical biogeography is in the midst of a scientific revolution. The aim of this paper is to analyze the evolution of historical biogeography during the first decade of the 21st century and its major trends in the near future. To achieve this objective we constructed a data base containing all articles which dealt with historical biogeography published in the Journal of Biogeography during 1998-2010. The data base included 610 published contributions. Our results indicated that historical biogeography is going through a growth period. The papers analysed were written by 2018 authors, with a mean of 3.3 authors per paper. Authors from 62 countries were involved, and most of them worked in Europe or North America. The Palaearctic was the most analysed region. Most contributions dealt with terrestrial habitats and were devoted to animal (especially Chordata) and plant taxa. Phylogeography was the most used approach (35%), followed by parsimony analysis of endemicity (13%) and molecular biogeography (12%), with cladistic biogeography and event-based methods at 6% each. Some of the future challenges that historical biogeography faces could be summarized as follows: 1) to increase the study of taxa which are underrepresented according to the segment  of biodiversity they represent, 2) to balance the amount of work devoted to different biogeographical regions, 3) to increase biogeographical knowledge of aquatic habitats, 4) to maintain the diversity of approaches, preventing the reduction of time, spatial, and taxonomic scales addressed by the discipline, and 5) to continue integrating historical biogeography along with other sources of information from other disciplines (e.g. ecology, palaeontology, geology, isotope chemistry, remote sensing) into a richer context for explaining past, present, and future patterns of biodiversity on Earth.","palabras_clave":"AUTHORSHIP|BIBLIOMETRY|BIOGEOGRAPHICAL REGIONS|HISTORICAL BIOGEOGRAPHICAL APPROACHES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63708,"idioma_id":4,"titulo":"Comparative anatomy of elaiophores and oil secretion in the genus Gomesa (Orchidaceae)","resumen":"Oils are atypical floral rewards in Orchidaceae, and are produced by epidermal glands called elaiophores. Flowers of many members of the sub-tribe Oncidiinae either produce oil or mimic oil-producing flowers of Malpighiaceae. We focus our research on three species of Gomesa, namely, G. flexuosa, G. riograndensis and G. varicosa in order to investigate the presence of elaiophores, their micromorphology and internal structure and the process of oil secretion. Elaiophores were located using Sudan III, and their internal and external features examined using light and scanning electron microscopy, respectively. The presence of elaiophores was confirmed for regions of calli that are prominent and exposed. In all three species, the secretory tissue comprised a single layer of epidermal cells, together with some parenchymatous, subsecretory cells. Epidermal cells were isodiametric or palisade-like, with dense cytoplasm, small vacuoles and prominent nuclei. Gomesa flexuosa differs from the other species investigated in that it bears intermediate elaiophores (i.e. epithelial and possessing unicellular, secretory trichomes). Based on the absence of a distended cuticle, we propose that oil possibly passes through the wall and cuticle as small lipid moieties. In addition, some evolutionary trends in the elaiophores in the genus Gomesa are suggested.","palabras_clave":"GOMESA|MICROMORPHOLOGY|OIL SECRETION|ONCIDIINAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63709,"idioma_id":5,"titulo":"Volcanismo alcalino involucrado en el proceso de apertura de la cuenca pensilvaniana, provincia de Mendoza","resumen":"Las rocas volcánicas inter-carboníferas de la Cordillera Frontal de Mendoza comprenden laAsociación Volcánica Meso-Varíscica, compuesta por diques (hasta 5 m de espesor) y coladas vesiculares de colores negruzcos. Estratigráficamente son pre-Pensilvaniano. Presentan textura intergranular a intersertal, compuesta por plagioclasa de hasta 2 mm (57%), entre los que se disponen clinopiroxeno (13%), opacos (12%), anfíbol de hasta 1 cm (10%), olivino, ocasionalmente cuarzo, feldespato potásico y epidoto; con apatito de hasta 1 mm como accesorio común. Químicamente son basaltos, traquibasaltos potásicos, mugearitas y hawaiitas, con una tendencia alcalina y afinidad con una suite de tipo Basanita-Fonolita. Registran una cristalización fraccionada de olivinoplagioclasa en los estadios iniciales, y de clinopiroxeno-plagioclasa en estadios más avanzados. Fueron generados en un ambiente tectónico de intraplaca a partir de la fusión parcial de un manto de composición lhertzolítica a harzburgítica, de signatura MORB, modificado por contaminación cortical. La relación K vs K\/Th* y la presencia de fases minerales hidratadas, sugieren a su vez un proceso de subducción y procesos de metasomatismo por fluidosasociados a subducción que brindan a estas rocas una firma de arco magmático continental. Su signatura geoquímica es equivalente a los basaltos del Rift Africano, con algunas variaciones similares a las registradas en los diversos brazos de la mega-estructura. Sin embargo, la complejidad de la distribución geoquímica de los elementos genera la necesidad de datos isotópicos que confirmen la interpretación presentada. Este episodio magmático ocurridobajo un régimen extensional (rifting) marca el inicio de la cuenca marina carbonífera superior de la Formación Alto Tunuyán, es equivalente al registrado en otras localidades a lo largo del eje cordillerano e implica un evento de extensión generalizado en el margen occidental de Gondwana limitado estratigráficamente por la Fase Diastrófica Chánica (Devónico superior) y el arco volcánico Permo-Triásico (Grupo Choiyoi).","palabras_clave":"BASALTOS|INTRAPLACA|PRE-PENSILVANIANO|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63710,"idioma_id":4,"titulo":"High Resolution Para-Hydrogen Induced Polarization in Inhomogeneous Magnetic Fields","resumen":"The application of parahydrogen for the generation of hyperpolarization has increased continuously during the  last years. When the chemical reaction is carried out at the same field as the NMR experiment (PASADENA protocol)  an antiphase signal is obtained, with a separation of the resonance lines of a few Hz. This imposes a stringent limit to  the homogeneity of the magnetic field in order to avoid signal cancellation. In this work we detect the signal arising  from hyperpolarized Hexene by means of a CPMG pulse train. After Fourier transformation the obtained J-Spectra  not only presents an enhanced spectral resolution but also avoids partial peak cancellation.","palabras_clave":"RMN|PHIP|Espectroscopia|Hiperpolarizacion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63711,"idioma_id":4,"titulo":"Morphology of the claw closer muscle of two estuarine crab species (Crustacea, Varunidae): an ultrastructural study","resumen":"We analyzed the ultrastructural features of the claw closer muscles in two estuarine crabs, Cyrtograpsus angulatus and Neohelice granulata, by transmission electron microscopy. Adult male crabs at intermolt stage were collected in the Mar Chiquita Coastal Lagoon (Buenos Aires, Argentina). The muscle fibers of both species showed evident striations, peripheral and intermyofibrillar nuclei, clefts in continuity with T and Z tubules, sarcoplasmic reticulum and T tubules forming dyads and triads usually located between the A and I bands, and mitochondria located mainly beneath the sarcolemma. Glycogen was observed as diffuse, small particles among myofilaments. The claw closer muscle of C. angulatus exhibited two fiber types: one with relatively fast-contracting fibers (shorter sarcomeres, myofilaments with an ordered arrangement, lineal Z discs, a well-developed sarcotubular system) and fatigue-resistant (numerous large mitochondria); and the other type, with slower-contracting fibers (longer sarcomeres, less orderly arranged myofilaments, wavy Z discs, a less developed sarcotubular system) and less resistant to fatigue (lower mitochondrial density). N. granulata showed only the slow, less resistant to fatigue type. The fibers less resistant to fatigue and more slowly contracting would presumably be used primarily for displays and agonistic interactions, whereas fast fibers with abundant mitochondria would be associated with continuous movements during feeding and grooming.","palabras_clave":"ULTRASTRUCTURE|CLAW CLOSER MUSCLE|CYRTOGRAPSUS ANGULATUS|NEOHELICE GRANULATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63712,"idioma_id":4,"titulo":"Brucella abortus induces intracellular retention of MHC-I molecules in human macrophages down-modulating cytotoxic CD8(+) T cell responses.","resumen":"Brucella abortus elicits a vigorous Th1 immune response which activates cytotoxic T lymphocytes. However, B. abortus persists in its hosts in the presence of CD8+ T cells, establishing a chronic infection. Here, we report that B. abortus infection of human monocytes\/macrophages inhibited  the IFN-g-induced MHC-I cell surface expression. This phenomenon was dependent on metabolically active viable bacteria. MHC-I down-modulation  correlated with the development of diminished CD8+ cytotoxic T cell response as evidenced by the reduced expression of the activation marker CD107a on CD8+ T lymphocytes and a diminished percentage of IFN-g producing CD8+ T cells. Inhibition of MHC-I expression was not due to changes in protein synthesis. Rather, we observed that upon B. abortus infection MHC-I molecules were retained within the Golgi apparatus. Overall, these results describe a novel mechanism based on the intracellular sequestration of MHC-I molecules whereby B. abortus would avoid CD8+ cytotoxic T cell responses, evading their immunological  surveillance.","palabras_clave":"EVASION|INTERFERON|CONFOCAL|BRUCELLA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63713,"idioma_id":4,"titulo":"Brucella abortus induces TNF-&#945;-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases","resumen":"Background: Central nervous system (CNS) invasion by bacteria of the genus Brucella results in an inflammatorydisorder called neurobrucellosis. We have recently demonstrated that B. abortus infects microglia and astrocytes,eliciting the production of a variety of pro-inflammatory cytokines which contribute to CNS damage. Matrixmetalloproteinases (MMP) have been implicated in inflammatory tissue destruction in a range of pathologicalsituations in the CNS. Increased MMP secretion is induced by pro-inflammatory cytokines in a variety of CNSdiseases characterized by tissue-destructive pathology.Methods: In this study, the molecular mechanisms that regulate MMP secretion from Brucella-infected astrocytesin vitro were investigated. MMP-9 was evaluated in culture supernatants by ELISA, zymography and gelatinolyticactivity. Involvement of mitogen-activated protein kinases (MAPK) signaling pathways was evaluated by Westernblot and using specific inhibitors. The role of TNF-&#945; was evaluated by ELISA and by assays with neutralizingantibodies.Results: B. abortus infection induced the secretion of MMP-9 from murine astrocytes in a dose-dependent fashion.The phenomenon was independent of bacterial viability and was recapitulated by L-Omp19, a B. abortuslipoprotein model, but not its LPS. B. abortus and L-Omp19 readily activated p38 and Erk1\/2 MAPK, thus enlistingthese pathways among the kinase pathways that the bacteria may address as they invade astrocytes. Inhibition ofp38 or Erk1\/2 significantly diminished MMP-9 secretion, and totally abrogated production of this MMP when bothMAPK pathways were inhibited simultaneously. A concomitant abrogation of B. abortus- and L-Omp19-inducedTNF-&#945; production was observed when p38 and Erk1\/2 pathways were inhibited, indicating that TNF-&#945; could beimplicated in MMP-9 secretion. MMP-9 secretion induced by B. abortus or L-Omp19 was completely abrogatedwhen experiments were conducted in the presence of a TNF-&#945; neutralizing antibody. MMP-9 activity was detectedin cerebrospinal fluid (CSF) samples from patients suffering from neurobrucellosis.Conclusions: Our results indicate that the inflammatory response elicited by B. abortus in astrocytes would lead tothe production of MMP-9 and that MAPK may play a role in this phenomenon. MAPK inhibition may thus beconsidered as a strategy to control inflammation and CNS damage in neurobrucellosis","palabras_clave":"BRUCELLA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63714,"idioma_id":4,"titulo":"Phenotypic and genotypic characteristics of Neisseria meningitidis disease-causing strains in Argentina, 2010","resumen":"Phenotypic and genotypic characterization of 133 isolates of Neisseria meningitidis obtained from meningococcal diseasecases in Argentina during 2010 were performed by the National Reference Laboratory as part of a project coordinated bythe PAHO within the SIREVA II network. Serogroup, serotype, serosubtype and MLST characterization were performed.Minimum Inhibitory Concentration to penicillin, ampicillin, ceftriaxone, rifampin, chloramphenicol, tetracycline andciprofloxacin were determined and interpreted according to CLSI guidelines. Almost 49% of isolates were W135, and twoserotype:serosubtype combinations, W135:2a:P1.5,2:ST-11 and W135:2a:P1.2:ST-11 accounted for 78% of all W135 isolates.Serogroup B accounted for 42.1% of isolates, and was both phenotypically and genotypically diverse. Serogroup C isolatesrepresented 5.3% of the dataset, and one isolate belonging to the ST-198 complex was non-groupable. Isolates belongedmainly to the ST-11 complex (48%) and to a lesser extent to the ST-865 (18%), ST-32 (9,8%) and the ST-35 complexes (9%).Intermediate resistance to penicillin and ampicillin was detected in 35.4% and 33.1% of isolates respectively. TwoW135:2a:P1.5,2:ST-11:ST-11 isolates presented resistance to ciprofloxacin associated with a mutation in the QRDR of gyrAgene Thr91-Ile. These data show serogroup W135 was the first cause of disease in Argentina in 2010, and was stronglyassociated with the W135:2a:P1.5,2:ST-11 epidemic clone. Serogroup B was the second cause of disease and isolatesbelonging to this serogroup were phenotypically and genotypically diverse. The presence of isolates with intermediateresistance to penicillin and the presence of fluorquinolone-resistant isolates highlight the necessity and importance ofmaintaining and strengthening National Surveillance Programs.","palabras_clave":"NEISSERIA|MENINGITIDIS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63715,"idioma_id":4,"titulo":"Glutathione-S-transferase (GST) polymorphisms are associated with relapse after radical prostatectomy","resumen":"Background:Organ confined prostate cancer (PCa) can be cured by radical retropubic prostatectomy (RRP); however, some tumors will still recur. Current tools fail to identify patients at risk of recurrence. Glutathione-S- transferases (GSTs) are involved in the metabolism of carcinogens, hormones and drugs. Thus, genetic polymorphisms that modify the GST activities may modify the risk of PCa recurrence.Methods:We retrospectively recruited Argentine PCa patients treated with RRP to study the association between GST polymorphisms and PCa biochemical relapse after RRP. We genotyped germline DNA in 105 patients for: GSTP1 c.313A>G (p.105 Ile>Val, rs1695) by PCR-RFLP; and GSTT1 null and GSTM1 null polymorphisms by multiplex PCR. Kaplan-Meier curves and Cox proportional hazard models were used to evaluate these associations.Results: Patients with GSTP1 c.313GG genotype showed shorter biochemical relapse-free survival (BRFS) (P=0.003) and higher risk for recurrence in unadjusted (Hazard ratio (HR)=3.16, 95% confidence interval (95% CI)=1.41-7.06, P=0.005) and multivariate models (HR=3.01, 95% CI=1.13-8.02, P=0.028). We did not find significant associations for GSTT1 and GSTM1 genotypes. In addition, we found shorter BRFS (P=0.010) and increased risk for recurrence for patients having two or more risk alleles when we combined the genotypes of the three GSTs in multivariate models (HR=3.06, 95% CI=1.20-7.80, P=0.019).Conclusions:Our results give support to the implementation of GSTs genotyping for personalized therapies as a novel alternative for PCa management for patients who undergo RRP. To the best of our knowledge, this is the first study that examined GST polymorphisms in PCa progression in Argentine men. Replication of our findings in larger cohort is warranted. © 2013 Macmillan Publishers Limited. All rights reserved.","palabras_clave":"BIOCHEMICAL RELAPSE|GLUTATHIONE-S-TRANSFERASE|GST|POLYMORPHISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63716,"idioma_id":4,"titulo":"Optimization of biodiesel production process using sunflower oil and tetramethyl ammonium hydroxide as catalyst","resumen":"In this work, the biodiesel production process by transesterification of sunflower oil was investigated using tetramethyl ammonium hydroxide (TMAH) as catalyst. The reaction conditions, such as catalyst concentration, temperature and reaction time were optimized in order to obtain a product that fulfills the quality requirements of international standards. It was found that the optimum reaction conditions were: temperature 60 °C, 1.76 wt% TMAH catalyst and 2 h reaction time. High amount of soaps were formed when using this catalyst. Therefore, an alternative purification methodology was used, especially useful for samples with high soaps content. This procedure consists of a first extraction stage using neutral water, which efficiently remove soaps. TMAH increases the solubility of mono and di-glycerides in the glycerine phase, thus decreasing their concentration in the biodiesel phase. Consequently, for a given production of mono- and di-glycerides, lower amount of these compounds were found in the final biodiesel product, being this an important advantage of this catalyst.","palabras_clave":"biodiesel|tetramethylammonium|sunflower oil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63717,"idioma_id":4,"titulo":"Direct effect of dsRNA mimetics on cancer cells induces endogenous IFN&#946; production capable of improving dendritic cells function.","resumen":"The use of viral double-stranded RNA (dsRNA) mimetics in cancer immunotherapy has been explored for several decades with the idea of enhancing innate and adaptive immune responses and altering the tumor microenvironment.  Polyinosine-polycytidylic acid (poly I:C) and Polyadenylic?polyuridylic acid (poly A:U) are synthetic analogs of viral dsRNA, which are strong inducers of a type I Interferon (IFN) response.  In this work, we describe a novel effect of dsRNA analogs on cancer cells: besides their potential of inducing cancer cell apoptosis through an IFN&#946; autocrine loop, dsRNA-elicited IFN&#61538; production is enough to improve dendritic cell functionality, which could in turn, improve the antitumoral immune response.  Human A549 lung and DU145 prostate carcinoma cells significantly respond to poly I:C stimulation, producing IFN&#61538;&#61472;, at levels that are capable of activating STAT1 and enhancing CD40 and CD86 expression on human monocyte-derived dendritic cells (Mo-DC). Also, IFN&#61538;&#61472;produced by&#61472;&#61472;poly I:C-activated human cancer cells  increase the capacity of Mo-DCs of stimulating IFN-&#947; production in an allogeneic stimulatory culture in vitro.  Moreover, when melanoma murine B16 cells were stimulated in vitro with poly A:U and then inoculated into TLR3 deficient mice, they elicited smaller tumors. This tumor growth inhibition was abrogated in IFNAR1-deficient mice.  Thus, dsRNA compounds are effective adjuvants not only because they activate DCs and promote strong adaptive immunity, but also because they can directly act on cancer cells to induce endogenous IFN&#61538; production and contribute to the antitumoral immune response.","palabras_clave":"DENDRITIC CELLS |DSRNA|IFN-B|TLR3|TUMOR IMMUNOLOGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63718,"idioma_id":4,"titulo":"New hydrogel obtained from a novel dendritic monomer as a promising candidate for biomedical applications","resumen":"Acid functional hydrogels are a type of materials with many advantages. Over the last years, increasing attention for the synthesis of dendronized polymers has been drawn due to their unique properties of high multivalence in the same surface as compared to conventional polymers. In this study, we report the preparation of novel acid dendronized hydrogels using a dendritic monomer obtained from Behera?s amine. The swelling and rheological performance, the non-toxicity over fibroblast cells and the drug encapsulation capacity of the novel hydrogels suggests that the new materials can achieve great potential as carrier for drug delivery and other potential biomedical applications","palabras_clave":"BIOMATERIALS|DENDRONIZED POLYMERS|DRUG CARRIERS|HYDROGELS|SOFT MATERIALS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63719,"idioma_id":4,"titulo":"Modeling of a bench-scale photocatalytic reactor for water disinfection from laboratory-scale kinetic data","resumen":"A model of a bench-scale, annular, continuous flow reactor for the photocatalytic disinfection of water,operated under conditions of no perfect mixing is presented. The performance of the photoreactor has  been simulated following a predictive procedure, with no adjustable parameters, based on the intrinsic kinetics and the information about the geometry, irradiation source and operation conditions (catalysts concentration and its properties as well as the initial concentration of bacteria). The only experimental information required to be determined at laboratory scale are the kinetic parameters of a mechanistic model that takes into account the explicit dependence of the reaction rate with respect to the local volumetric rate of photon absorption (LVRPA) and the optical properties of the catalyst. The proposed model predicts an optimal catalyst concentration in the range 0.1-0.2  10-3 g\/cm3, with a significant decrease in the disinfection efficiency for higher catalyst loadings. Important resistances to the mass transport are detected at high TiO2 concentrations, as a result of the low diffusion coefficient of the employed microorganism (Escherichia coli). Operating under the optimal catalyst concentration, model predictions show satisfactory agreement with experimental results extracted from the custom-built bench scale reactor.","palabras_clave":"PHOTOCATALYSIS|DISINFECTION|PHOTOREACTOR|MASS TRANSPORT|KINETICS|E. COLI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63720,"idioma_id":5,"titulo":"Las condiciones de trabajo de los asalariados de la esquila patagónica argentina.","resumen":"En un contexto de globalización de los mercados productivos agropecuarios y de una actividad  económica destinada a la exportación, este artículo propone analizar las condiciones del trabajo de  los asalariados en la esquila de ovinos en Chubut y sus posibles modificaciones respecto de las  características históricas. Desde fines del siglo XIX, la producción de lana ovina en Chubut fue de  gran importancia económica y organizadora principal del mercado laboral de la zona. Partiendo de  una revisión histórica del desarrollo de la actividad lanera, se considerarán tanto las regulaciones  laborales, las políticas públicas y la presencia gremial, como las características y la dinámica propias  de este mercado de trabajo, para comprender los cambios y continuidades en las condiciones  laborales de los esquiladores. Este artículo surge de una investigación grupal e involucra información  de diversas fuentes, obtenida principalmente mediante entrevistas, encuestas, observaciones en  campo y otros registros realizados durante casi una década en torno a esta temática, en ésta y otras  zonas del país.","palabras_clave":"Mercado de trabajo rural |Esquila|Condiciones de trabajo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63721,"idioma_id":6,"titulo":"Bref parcours à travers l'histoire de la presse satirique argentine: de ses débuts au XIXe siècle jusqu'à nos jours","resumen":"On pourrait dire que l´Argentine est née avec une plume et un crayon à la main, car les premières publications satiriques remontent avant la consolidation de la Nation. Leur histoire est marquée par des périodes de développement et d?autres d´extrême difficulté, lorsque l?interdiction des médias d?opposition, la censure et les menaces étaient monnaie courante. Les cinq coups d´État qui se sont succédé au cours du XXe siècle ont sans doute laissé leur trace dans le développement de la presse en question. Voici un parcours de l?histoire satirique et humoristique du pays à travers quelques-unes des publications les plus marquantes depuis XIXe siècle jusqu´à nos jours.","palabras_clave":"prensa satírica|Argentina|historia medios"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63722,"idioma_id":4,"titulo":"Sequential combined treatment with allopurinol and benznidazole in the chronic phase of Trypanosoma cruzi infection: a pilot study.","resumen":"OBJECTIVES:  Even though the use of combined drugs has been proved to be effective in other chronic infections, assessment of combined treatment of antiparasitic drugs in human Chagas´ disease has not been performed. Herein, a pilot study was conducted to evaluate the tolerance and side effects of a sequential combined treatment of two antiparasitic drugs, allopurinol and benznidazole, in the chronic phase of Trypanosoma cruzi infection.  PATIENTS AND METHODS:  Changes in total and T. cruzi-specific T and B cells were monitored during a median follow-up of 36 months. Allopurinol was administered for 3 months (600 mg\/day) followed by 30 days of benznidazole (5 mg\/kg\/day) in 11 T. cruzi-infected subjects.  RESULTS:  The combined sequential treatment of allopurinol and benznidazole was well tolerated. The levels of T. cruzi-specific antibodies significantly decreased after sequential combined treatment, as determined by conventional serology and by a multiplex assay using recombinant proteins. The frequency of T. cruzi-specific interferon-&#947;-producing T cells significantly increased after allopurinol treatment and decreased to background levels following benznidazole administration in a substantial proportion of subjects evaluated. The levels of total naive (CD45RA + CCR7 + CD62L+) CD4 + and CD8 + T cells were restored after allopurinol administration and maintained after completion of the combined drug protocol, along with a decrease in T cell activation in total peripheral CD4 + and CD8 + T cells.  CONCLUSIONS:  This pilot study shows that the combination of allopurinol and benznidazole induces significant modifications in T and B cell responses indicative of a reduction in parasite burden, and sustains the feasibility of administration of two antiparasitic drugs in the chronic phase of Chagas´ disease.","palabras_clave":"Sequential combined treatment | allopurinol and benznidazole .|the chronic phase|Trypanosoma cruzi infection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63723,"idioma_id":4,"titulo":"Clinical characteristics and outcome of 107 adult patients with chronic Chagas disease and parasitological cure criteria","resumen":"BACKGROUND: The cure in adult patients with chronic Chagas disease and the relationship between parasitological and clinical evolution is still under debate. The aim of this study was to analyze the clinical, epidemiological and progression features of the disease in a patient population who became serologically negative either spontaneously or post-etiological treatment. METHODS: We included 107 patients over 20 years old with three different confirmed reactive anti-Trypanosoma cruzi serologic tests on admission, and a minimum of two years of follow-up. Patients were assigned to clinical groups according to Kuschnir. Change of clinical group was considered a heart disease progression criterion, and seronegative conversion of two or three as parasitological cure criterion. RESULTS: From 107 patients with parasitological cure, 82 had received treatment (77%) and 25 became spontaneously seronegative (23%). Forty-six (43%) and 61 (57%) patients had two and three negative serological tests, respectively. No differences in clinical groups, ECG, echocardiogram and heart disease progression were found in patients who became negative spontaneously or post-treatment. The clinical progression and ECG changes were observed in 5\/107 (5%) and 11\/107 (10%) respectively, in a mean of 10 years follow-up. CONCLUSIONS: Adults with chronic Chagas disease can cure, mostly post-etiological treatment, but also spontaneously, showing a favourable clinical outcome.","palabras_clave":"Benznidazole|Chagas disease|Disease progression|Parasitological cure|Spontaneous seroconversion|Treatment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63724,"idioma_id":5,"titulo":"Regeneración de plantas leñosas en el Chaco semiárido argentino: relación con factores bióticos y abióticos en micrositios creados por el aprovechamiento forestal","resumen":"La cosecha forestal genera micrositios que pueden ser colonizados por distintas especies de plantas leñosas, donde factores bióticos y abióticos pueden influir en la regeneración. En este trabajo se describen tres micrositios creados por la tala (caminos principales, vías de saca y claros de extracción) en dos sitios con distinto tiempo de recuperación (uno y dos años) del Chaco semiárido, Argentina. Se compara la riqueza y diversidad de plantas leñosas y se evalúa la relación de la densidad de plantas en función del tipo de micrositio, tiempo de recuperación, dureza del suelo, cobertura del dosel y cobertura de herbáceas. En los tres micrositios, la composición y riqueza de especies fueron similares, dominados por las mismas especies. Los caminos tuvieron suelos más compactados, las vías de saca doseles más cerrados y los claros suelos con mayor cobertura herbácea. La densidad de leñosas dependió de la interacción de las co-variables (dureza del suelo, coberturas del dosel y de herbáceas) con los micrositios y el tiempo de recuperación. La densidad en los caminos fue baja y se relacionó negativamente con la dureza del suelo y positivamente con las coberturas del dosel y de herbáceas, sugiriendo una interacción positiva en los micrositios más afectados. La regeneración de las dos especies de mayor valor forestal fue baja en comparación a otras especies; Aspidosperma quebracho-blanco tuvo una densidad similar y Schinopsis lorentzii mayor en comparación a la regeneración de la matriz circundante. Sin embargo, este resultado debe interpretarse con cuidado debido a la baja constancia de ambas especies (proporción de parcelas con un cierto taxón). Se propone, con cautela, que los micrositios generados no estarían afectando negativamente a la regeneración de las dos especies de mayor valor forestal de este ambiente.","palabras_clave":"CAMINOS|CLAROS|COBERTURA DEL DOSEL|DUREZA DEL SUELO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63725,"idioma_id":4,"titulo":"Long-distance bird migration within South America revealed by light-level geolocators","resumen":"        Little  is known about the timing of migration, migration routes, and migratory connectivity  of most of the &gt;230 species of birds that breed at south temperate latitudes  of South America and then migrate towards the tropics to overwinter. We used  light-level geolocators to track the migration of three male and three female  Fork-tailed Flycatchers (Tyrannus savana)  captured on their breeding territories in Argentina. All birds initiated fall  migration between late January and late February, and migrated 45 to 66 km day-1  in a northwesterly direction through central South America to either one or two  wintering areas. Five individuals first spent several weeks (in April and May)  in western Amazonia (mainly Peru, northwestern Brazil and southern Colombia),  before they movedeast to spend the rest of the nonbreeding season in central  Venezuela and northern Brazil. One individual occupied primarily one wintering  area in eastern Colombia, northwestern Brazil, and southwestern Venezuela. Fall  migration took approximately 7\u201312 weeks to complete, and covered  a distance of 2,888\u20134,105 km. We did not analyze  spring migration data because of broad overlap with the  austral spring equinox.These  results represent the first data on the wintering locations, migration timing  and routes of individual migrant passerine birds that breed in South America.  Given the general lack of similar data for practically all migratory birds that  breed in South America, geolocator technology has the potential to  revolutionize our understanding of how birds migrate&#8213;and the threats they face&#8213;on  South America\u2019s rapidly changing landscape.        Key  words: Argentina, Fork-tailed Flycatcher, geolocator, Neotropical austral  migrants, Tyrannus savana,  Tyrannidae.  ","palabras_clave":"ARGENTINA|FORK-TAILED FLYCATCHER|GEOLOCATOR|NEOTROPICAL AUSTRAL MIGRANTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63726,"idioma_id":4,"titulo":"Abnormalities in amphibian populations inhabiting agroecosystems in northeastern Buenos Aires Province, Argentina","resumen":"ABSTRACT: The occurrence of abnormalities in amphibians has been reported in many populations,  and its increase could be related to environmental pollution and habitat degradation. We  evaluated the type and prevalence of abnormalities in 5 amphibian populations from agroecosystems  with different degrees of agricultural disturbance (cultivated and reference areas). We  detected 9 types of abnormalities, of which the most frequent were those occurring in limbs. The  observed prevalence of abnormality in assessed populations from cultivated and reference areas  was as follows: Rhinella fernandezae (37.1 and 10.2%, respectively), Leptodactylus latrans adults  (28.1 and 9.2%) and juveniles (32.9 and 15.3%), and Hypsiboas pulchellus (11.6 and 2.8%). Scinax  granulatus populations did not show abnormalities. Pseudis minuta, which was only detected in  the reference area, exhibited a prevalence of 13.3%. For R. fernandezae, L. latrans, and H. pulchellus,  generalized linear mixed models showed that prevalence of abnormalities was significantly  higher (p < 0.05) in cultivated than in reference areas. L. latrans juveniles were more vulnerable  to abnormalities than adults (p < 0.05). The presence of abnormalities in some species  inhabiting different agroecosystems suggests that environmental stress factors might be responsible  for their occurrence. While we detected pesticides (endosulfan, cypermethrin, and chlorpyrifos)  and lower dissolved oxygen levels in ponds of the cultivated area, no data are currently available  on how other factors, such as injuries from predators and parasite infections, vary by land use.  Further research will be necessary to evaluate possible causes of abnormalities detected in the  present study mainly in the context of factor interactions.  K","palabras_clave":"AGRICULTURAL DISTURBANCE|PESTICIDE|BRACHYDACTYLY|ECTRODACTYLY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63727,"idioma_id":4,"titulo":"Gliotoxinogenic Aspergillus fumigatus in the dairy herd environment","resumen":"Abstract The potential association between hygienic conditions  in the environment of lactating cows and the presence  of gliotoxinogenic Aspergillus fumigatus strains was  studied. Milk samples (individual cow?s milk [ICM], bulk  tank milk [BTM]) from 44 dairy farms were sampled. In  ICM samples, eight different species of Aspergillus were  identified. A. flavus and A. fumigatus were predominant,  with 37.8 % and 26.1 % relative densities, respectively. A.  fumigatus strains were isolated from 61.4 % of the BTM  samples, and 34 % of these strains were able to produce  gliotoxin. Principal component analysis was used to associate  the presence of A. fumigatus with some hygienic and  sanitary practices. A significant and positive correlation was  observed between dry cow therapy and forestripping. The  presence of A. fumigatus gliotoxin producers in milk was  associated with high somatic cells count (SCC) samples.  Good hygienic and sanitary practices were associated with  absence of A. fumigatus and relatively low SCCs of  <250,000 cells\/ml. In general, a high percentage of dairy  farms were positive for A. fumigatus in BTM samples. This is the first work that indicates the positive effects of adequate  hygienic and sanitary practices in dairy herds on the  control of A. fumigatus and related species. By reducing the  frequency of Aspergillus spp. in the dairy environment, the  risk of farm handlers? exposure and the risk of  intramammary fungal infections would also be reduced.","palabras_clave":"Aspergillus fumigatus . |Gliotoxin |Somatic cell count . |Milk hygiene |Dairy cow"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63728,"idioma_id":4,"titulo":"Rotor demagnetization effects on permanent magnet synchronous machines","resumen":"The effects of asymmetrical magnet faults on the rotor of permanent magnet synchronousmachines (PMSM) are analyzed in the present work. In particular, demagnetization effects areanalyzed using the finite element method. Two different stator winding configurations, series andparallel connected windings, are considered in the analysis. From PMSM with parallel windings, a faultdetection strategy based on the information contained in winding-current spectrum is proposed. Afault severity factor is also proposed to quantify demagnetization on a single magnet. This factor ispractically independent from the motor load conditions. Additionally, voltage reduction and currentderating due to rotor demagnetization are also analyzed. Experimental results are presented tovalidate the proposal.","palabras_clave":"Permanent magnet synchronous machine|Demagnetization|Fault detection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63729,"idioma_id":4,"titulo":"Top-antitop resonance searches beyond 1 TeV","resumen":"We perform a general parton level analysis for the search of heavy resonant states in the production of tt&#773;         pairs at the LHC with an integrated luminosity of 30&#8201;&#8201;fb-1.   We assume the existence of resonances that only couple to quarks and   propose kinematic cuts in order to increase the number of events   produced through quark annihilation. We study the interplay between   different variables and their impact on the purity of the selected   sample. We focus on the longitudinal (&#946;) and transverse (pT) momenta of the tt&#773;         pair and the scattering angle (&#952;) in the center of mass reference frame. We observe that &#946; is replaced by &#952;   as a suitable discriminating variable of quark-annihilation processes   for invariant masses above 1&nbsp;TeV. Finally, we illustrate the analysis   with a gluon resonance of 1.5&nbsp;TeV and show the improvement in the   sensitivity of the signal when cuts on &#952; are imposed.","palabras_clave":"invariant-spectrum|new physics|TeV|lhc"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63730,"idioma_id":4,"titulo":"Therapeutic inorganic ions in bioactive glasses to enhance bone formation and beyond","resumen":"Bioactive glasses (BG) are being widely used for bone tissue engineering applications due to their bioactivity (ability to form strong bonds to bone) and their stimulating effects on bone formation. Recently, progress has been made to enhance the biological impact of BGs by incorporating specific metallic ions in silicate (or phosphate) glasses, including boron, copper, cobalt, silver, zinc and strontium. This review summarizes the newest developments on novel compositions of bioactive glasses in the field of bone tissue engineering related to osteogenesis and angiogenesis. Furthermore, new applications areas for bioactive glasses, including nerve regeneration and cancer treatment, are highlighted.","palabras_clave":"El trabajo no posee palabras claves"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63731,"idioma_id":4,"titulo":"Single-step electrochemical deposition of antimicrobial orthopeaedic coatings based on bioactive glass\/chitosan\/nano-silver composite system","resumen":"Composite orthopaedic coatings with antibacterial capability containing chitosan, Bioglass® particles (9.8ìm) and silver nanoparticles (Ag-np) were fabricated using a single-step electrophoretic deposition (EPD) technique, and their structural and preliminary in vitro bactericidal and cellular properties were investigated. Stainless steel 316 was used as a standard metallic orthopaedic substrate. The coatings were compared with EPD coatings of chitosan and chitosan\/Bioglass®. The ability of chitosan as both a complexing and stabilizing agent was utilized to form uniformly deposited Ag-np. Due to the presence of Bioglass® particles, the coatings were bioactive in terms of forming carbonated hydroxyapatite in simulated body fluid (SBF). Less than 7wt.% of the incorporated silver was released over the course of 28days in SBF and the possibility of manipulating the release rate by varying the deposition order of coating layers was shown. The low released concentration of Ag ions (<2.5ppm) was efficiently antibacterial against Staphyloccocus aureus up to 10days. Although chitosan and chitosan\/Bioglass® coating supported proliferation of MG-63 osteoblast-like cells up to 7days of culture, chitosan\/Bioglass®\/Ag-np coatings containing 342 ìg of Ag-np showed cytotoxic effects. This was attributed to the relatively high concentration of Ag-np incorporated in the coatings.","palabras_clave":"COMPOSITE COATINGS|BIOGLASS|CHITOSAN|SILVER NANOPARTICLES|ELECTROPHORETIC DEPOSITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63732,"idioma_id":4,"titulo":"Composite polymer-bioceramic scaffolds with drug delivery capability for bone tissue engineering","resumen":"Introduction: Next-generation scaffolds for bone tissue engineering (BTE)  should exhibit the appropriate combination of mechanical support and morphological guidance for cell proliferation and attachment while at the same time serving as matrices for sustained delivery of therapeutic drugs and\/or biomolecular signals, such as growth factors. Drug delivery from BTE scaffolds to induce the formation of functional tissues, which may need to vary temporally and spatially, represents a versatile approach to manipulating the local environment for directing cell function and\/or to treat common bone diseases or local infection. In addition, drug delivery from BTE is proposed to either increase the expression of tissue inductive factors or to block the expression of others factors that could inhibit bone tissue formation.  Composite scaffolds which combine biopolymers and bioactive ceramics in  mechanically competent 3D structures, including also organic--inorganic  hybrids, are being widely developed for BTE, where the affinity and interaction between biomaterials and therapeutic drugs or biomolecular signals play a decisive role in controlling the release rate.  Areas covered: This review covers current developments and applications of 3D composite scaffolds for BTE which exhibit the added capability of controlled delivery of therapeutic drugs or growth factors. A summary of drugs and biomolecules incorporated in composite scaffolds and approaches developed to combine biopolymers and bioceramics in composites for drug delivery systems for BTE is presented. Special attention is given to identify the main challenges and unmet needs of current designs and technologies for developing such multifunctional 3D composite scaffolds for BTE.  Expert opinion: One of the major challenges for developing composite scaffolds for BTE is the incorporation of a drug delivery function of sufficient complexity to be able to induce the release patterns that may be necessary for effective osseointegration, vascularization and bone regeneration. Loading 3D scaffolds with different biomolecular agents should produce a codelivery system with different, predetermined release profiles. It is also envisaged that the number of relevant bioactive agents that can be loaded onto scaffolds will be increased, whilst the composite scaffold design should exploit synergistically the different degradation profiles of the organic and inorganic components.","palabras_clave":"BONE TISSUE ENGINEERING|COMPOSITE SCAFFOLDS|DRUG DELIVERY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63733,"idioma_id":4,"titulo":"Indigenous PAH-Degrading Bacteria from oil-polluted   sediments in Caleta Cordova, Patagonia Argentina","resumen":"Indigenous bacteria with the capability to degrade polycyclic aromatic hydrocarbons (PAH) were isolated from polluted sediment samples recovered from Caleta Cordova by using selective enrichment cultures supplemented with phenanthrene. Bacterial communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in order to detect changes along enrichment culture and relationships with the representative strains subsequently isolated. Members of these communities included marine bacteria such as Lutibacter, Polaribacter, Arcobacter and Olleya, whose degradation pathway of PAH has not been studied yet. However, isolated bacteria obtained from this enrichment comprised the genus Pseudomonas,  Marinobacter, Salinibacterium and Brevibacterium. The ability of isolates to grow and degrade naphthalene, phenanthrene and pyrene was demonstrated by detection of the residual substrate by HPLC. Archetypical naphthalene and catechol dioxygenase genes were found in two isolates belonging to  genus Pseudomonas (Pseudomonas monteilii P26 and Pseudomonas xanthomarina N12), suggesting biodegradation potential in these sediments. The successful bacterial isolation with the ability to degrade PAH in pure culture suggest the possibility to study and further consider strategies like growth stimulation in situ, in order to increase the intrinsic bioremediation opportunities in the polluted Caleta Cordova harbor.","palabras_clave":"Biodegradation|Caleta Cordova|PHA|polluted sediment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63734,"idioma_id":4,"titulo":"Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation","resumen":"&nbsp;In Antarctica, the environmental conditions and the restrictions imposed by the Antarctic Treaty prevent inoculation with foreign bacteria. Therefore, our aim was to investigate native bacterial consortia which might serve to design bacterial formulas suitable for soil bioremediation processes at cold temperatures. Two bacterial consortia, M10 and J13, were isolated from diesel contaminated Antarctic soils. Their ability to use hydrocarbons was evaluated in vitro and by the detection of three catabolic genes (alkB, nahAc, xylE). Both consortia showed similar 16S rRNA gene profiles, suggesting the presence of the same phylotypes. Total 16S rDNA was cloned from M10 grown on diesel. Sixty clones were screened, grouped by restriction profiles of PCR-amplified inserts and sequenced. T-RFLP (Terminal-Restriction-Fragment-Length-Polymorphism) of clones showed that all phylotypes from the entire consortia were recovered. A culture-dependent approach was used to isolate M10 components able to utilise aliphatic and aromatic hydrocarbons. Pseudomonas, Stenotrophomonas, Pedobacter and Brevundimonas genera were detected. The combination of dependent and independent culture methods allowed elucidating the taxonomic composition of these&nbsp; native bacterial consortia. Further work will assess whether combining the isolates obtained as a defined mixed culture can enhance bioremediation of contaminated soils. &nbsp; ","palabras_clave":"bacterial consortium|Antarctica|T-RFLP|Bioremediation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63735,"idioma_id":4,"titulo":"Detecting Pedestrians on a Movement Feature Space","resumen":"This work aims at detecting pedestrians in surveillance video sequences. A pre-processing step detects motion regions on the image using a scene background model based on level lines, which generates a Movement Feature Space, and a family of oriented histogram descriptors. A cascade of boosted classifiers generates pedestrian hypotheses using this feature space. Then, a linear Support Vector Machine validates the hypotheses that are likeliest to contain a person. The combination of the three detection phases reduces false positives, preserving the majority of pedestrians. The system tests conducted in our dataset, which contains low-resolution pedestrians, achieved a maximum performance of 25.5 % miss rate with a rate of of 0.1 false positives per image. This value is comparable to the best detection values for this kind of images. In addition, the processing time is between 2 and 6 fps on 640x480 pixel captures. This is therefore a fast and reliable pedestrian detector.","palabras_clave":"PATTERN RECOGNITION|FEATURE SPACE|LEVEL LINES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63736,"idioma_id":4,"titulo":"Ontogenetic diet shift in Commerson's dolphin (Cephalorhynchus commersonii commersonii) off Tierra del Fuego","resumen":"The Commerson´s dolphin (Cephalorhynchus c. commersonii, Lacépède, 1804) is the most common endemic dolphin from the coastal waters of Tierra del Fuego incidentally caught in artisanal coastal gill nets. A better understanding of its trophic ecology is essential to facilitate the effective management and conservation of it population. The  aim of this study was to analyze possible shifts in diet among age and sex classes by analyzing bone collagen &#948;13C and &#948;15N values collected from 220 individuals along the coast of Tierra del Fuego. Additional analysis of potential prey allowed us to use a stable isotope mixing model to quantify resource use. Isotopic comparisons suggest that all age classes share the same foraging areas. We found, however, a significant  dietary shift, juveniles consume a higher mean (±SD) proportion (60±7%) of pelagic Sprattus fuegensis than adults (36±10%), and the consumption of benthopelagic species such as Illex argentinus and Loligo gahi is higher in adults (33±12%) than juveniles (20±7%). This may result from an improvement in foraging skills and expansion of habitats to adulthood. While males move greater distances in search of mates and resources a lack of isotopic differences between the sexes suggests little evidence for sex-related resource partitioning. A significant decrease in &#948;15N values between calves and juveniles indicate a weaning period between 0.5 to 1 year as was shown in previous captive studies on this species. Our results also suggest that artisanal fisheries, despite being a major source of mortality, do not affect the long-term food habits of Commerson´s dolphin since they showed a weak trophic overlap.","palabras_clave":"STABLE ISOTOPES|DIET|CEPHALORHYNCHUS COMMERSONII COMMERSONII|SUBANTARCTIC WATERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63737,"idioma_id":4,"titulo":"Efficient Descriptor Tree Growing For Fast Action Recognition","resumen":"Video and image classification based on Instance-to-Class (I2C) distance attracted many recent studies, due to the good generalization capabilities it provides for non-parametric classifiers. In this work we propose a method for action recognition. Our approach needs no intensive learning stage, and its classification performance is comparable to the state-of-the-art. A smart organization of training data allows the classifier to achieve reasonable computation times when working with large training databases. An efficient method for organizing training data in such a way is proposed. We perform thorough experiments on two popular action recognition datasets: the KTH dataset and the IXMAS dataset, and we study the influence of one of the key parameters of the method on classification performance.","palabras_clave":"Action recognition| Nearest neighbor|Instance-to-Class distance"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63738,"idioma_id":4,"titulo":"A REVIEW ON INVASIVE PLANTS IN RANGELANDS OF ARGENTINA","resumen":"In Argentina, information on invasive plants is restricted to croplands, while no information is available on rangelands. Our objectives were to 1) identify and describe various characteristics of the most important non-native species that have become invasive and widespread in major rangeland territories of Argentina; 2) summarize the biological information about some naturalized, non-native species with potential to transform natural plant communities, and 3) discuss strategies for plant invasion management and biodiversity conservation in local rangeland ecosystems. The invasive species most represented at local, regional or state scale studies were Acroptilon repens, Centaurea solstitialis, Eleagnus angustifolia, Medicago minima, Chondrilla juncea, Dipsacus sativus and Sorghum halepense. Successful invasion and naturalization rates have been the result of several combined ecological traits: 1) capacity to produce allelophatic compounds, 2) deep rooting, 3) high module density, 4) rapid vegetative spread aboveground, 5) various traits that make species highly competitive, 6) tolerance to shading and water stress, 7) ability to take advantage of disturbances, 8) high seed production, germination and dispersal, and 9) high viability of residual seed banks. The determination of the abundance of invasive species at country scale, and their ecological and economical damage, are objectives of future research. This information will be a critical tool to make decisions on the need to control invasive species. Ecological studies providing understanding of the strategies which make an invader species a successful competitor are critical, and should be the first step to establish policies for control of invasive species and use of rangelands. &nbsp;","palabras_clave":"Argentina|Biodiversity|Invasive species|Rangelands|Weeds"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63739,"idioma_id":4,"titulo":"The effect of substrate diameter and incline on locomotion in an arboreal frog","resumen":"Frogs are characterized by a unique morphology associated with their saltatory lifestyle. Yet, arboreal species show morphological specializations relative to other ecological specialists allowing them to hold on to narrow substrates. However, almost nothing is known about the effects of substrate characteristics on locomotion in frogs. Here, we quantified the 3D kinematics of forelimb movement for frogs moving across branches of different diameters (1 and 40mm) and two different inclines (horizontal and 45deg uphill). Our results show that grip types differ while moving across substrates of differentdiameters and inclines. The kinematics of the wrist, elbow and shoulder as well as the body position relative to the substrate also showed significant effects of individual, diameter and incline. Kinematic differences involved duration, velocity of movement and angular excursions. Differences were most pronounced for the proximal joints of the forelimb and effects for substrate diameter were greater than for incline. Interestingly, the effects of diameter and incline on both grip type and kinematics are similar to what has been observed for lizards and primates, suggesting that the mechanics of narrow substrate locomotion drive the kinematics of movement independent of morphology and phylogeny.","palabras_clave":"KINEMATICS|MOVEMENT|FINE BRANCH MILIEU|GRASPING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63740,"idioma_id":4,"titulo":"Observations of UV radiation and total ozone column using ground based instruments in Río Gallegos, Argentina (51º 36' S, 69º 19' W)","resumen":"As a part of environmental studies in the southern hemisphere, the CEILAP Lidar Division with the financial support of JICA (Japan International Cooperation Agency) and the collaboration of IPSL France, mounted a ground based remote sensing site at Río Gallegos city (51º 36\u2019S, 69º 19\u2019W), at southern part of South America for the measurements of stratospheric ozone, with lidar remote sensing techniques and passive sensors to measure solar UV irradiance. The Patagonian region is characterized by high cloud cover during day changing strongly the distribution of UV radiation that reaches the ground surface. During the spring season some overpasses of ozone hole are masked by cloud cover avoiding the increase in UVB radiation. Solar UV radiation measured with multiband filter radiometer GUV-541 and Biometer manufactured by Biospherial Inc. San Diego and the Yankee Environmental Systems (YES) companies respectively. We present nine study days in the period 2007-2011 where total ozone column was below 250 DU focusing the impact that cloud cover had on the temporal evolution of these events.","palabras_clave":"UV index|Cloud cover|Ozone hole|Radiative transfer model"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63741,"idioma_id":4,"titulo":"Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells","resumen":"Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and metalloproteases activity on tumor (MCF-7 and IBH-7) and non-tumor (MCF-10A) human breast epithelial cell lines. Materials and methods: Human adipose tissues were obtained from patients and the conditioned medium from hATN and hATT collected after 24 h of incubation. MCF-10A, MCF-7 and IBH-7 cells were grown and incubated with CMs and proliferation and adhesion, as well as migration ability and metalloprotease activity, of epithelial cells after exposing cell cultures to hATN- or hATT-CMs were quantified. The statistical significance between different experimental conditions was evaluated by one-way ANOVA. Tukey0s post hoc tests were performed. Results: Tumor and non-tumor breast epithelial cells significantly increased their proliferation activity after 24 h of treatment with hATT-CMs compared to control-CMs. Furthermore, cellular adhesion of these two tumor cell lines was significantly lower with hATT-CMs than with hATNCMs. Therefore, hATT-CMs seem to induce significantly lower expression or less activity of the components involved in cellular adhesion than hATN-CMs. In addition, hATT-CMs induced pro-MMP-9 and MMP-9 activity and increased the migration of MCF-7 and IBH-7 cells compared to hATN-CMs. Conclusions: We conclude that the microenvironment of the tumor interacts in a dynamic way with the mutated epithelium. This evidence leads to the possibility to modify the tumor behavior\/phenotype through the regulation or modification of its microenvironment. We developed a model in which we obtained CMs from adipose tissue explants completely, either from normal or tumor breast. In this way, we studied the contribution of soluble factors independently of the possible effects of direct cell contact.","palabras_clave":"HUMAN BREAST ADIPOSE TISSUE|BREAST EPITHELIAL CELLS|CANCER|EPITHELIAL-STROMAL INTERACTIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63742,"idioma_id":4,"titulo":"Arbuscular mycorrhizal associations and dark septate endophytes in Yacon (Smallanthus sonchifolius) and a wild relative (Smallanthus macroscyphus)","resumen":"Mycorrhizal associations in Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson, Yacon, an ancient Andean crop and Smallanthus macroscyphus (Baker ex Martius) Grau, wild yacon, a close wild relative, are described for the first time. Yacon fibrous roots growing under field conditions have high levels of colonization by arbuscular mycorrhizal fungi (86%). Numerous vesicles filled with several types of lipid droplets were observed. Other fungi colonizing roots included dark septate endophytes (45%) and unidentified fungi that are probably saprophytic (25%) were observed. Only 9% of the samples analyzed were not colonized by any type of fungi. Glomus, Acaulospora, Scutellospora, Gigaspora and Pacispora were the main genera of arbuscular mycorrhiza identified. A similar high degree of mycorrhizal colonization was observed in Smallanthus macroscyphus in natural populations associated with Juglans australis native forest. The high level of mycorrhizal colonization and the low number of fine absorbing roots and the large roots diameters observed, suggest that both Smallanthus species are likely dependent on mycorrhiza.","palabras_clave":"SMALLANTHUS SONCHIFOLIUS|SMALLANTHUS MACROSCYPHUS|MYCORRHYZAS|VESICULAR ARBUSCULAR MYCORRHIZAS|DARK SEPTATE ENDOPHYTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63743,"idioma_id":4,"titulo":"LANDBIRD ASSEMBLAGES IN DIFFERENT AGRICULTURAL LANDSCAPES: A CASE STUDY IN THE PAMPAS OF CENTRAL ARGENTINA J","resumen":"The combination of countryside elements (e.g., crops and pasture lands, strip roadside vegetation, woodlots, temporary wetlands) increases landscape heterogeneity and defines different types of agricultural landscapes, from homogeneous landscapes dominated by a single countryside element (i.e., pure cropland or pure pastoral farming) to heterogeneous landscapes dominated by two or more countryside elements (i.e., mixed farming landscape). In this study we characterized landbird assemblages in different agricultural landscapes (cropland, pastoral and mixed landscapes) in the Pampas of central Argentina. We surveyed landbirds along transects to determine species richness, composition and abundance. Mixed landscapes had greater heterogeneity than pastoral and cropland landscapes. Mixed landscapes showed greater species richness than pastoral and cropland landscapes, particularly generalist species richness. On the other hand, cropland landscapes had lower grassland specialists richness (species of increased conservation concern in the Pampas) than pastoral and mixed landscapes. An indicator-species analysis supported the association of some grassland-specialists (Rhea americana, Asthenes hudsoni, Embernagra platensis, Pseudoleistes virescens) with pastoral landscapes. Our results suggest that maintaining landscape heterogeneity throughout the Pampas (i.e., mixed landscapes) is important for preserving bird species richness. However, it should point out that several threatened grassland specialists inhabiting the Pampas like Rhea Americana and Asthenes hudsoni are exclusively found at pastoral landscapes, so the long-term survival of these species will depend on the conservation of this landscape type","palabras_clave":"agroecosystem|bird species|land-use|matrix"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63744,"idioma_id":4,"titulo":"Breeding season habitat selection by Ferruginous Pygmy Owls Glaucidium brasilianum in central Argentina","resumen":"Capsule: The presence of Ferruginous Pygmy Owls Glaucidium brasilianum breeding in the xerophytic forest of Caldén Prosopis caldenia in central Argentina was slightly affected by forest maturity but neither by the structure of vegetation strata at the micro-habitat scale, nor by forest composition (mosaic of forest?grassland or shrubland) or proximity of water bodies at the macro-habitat scale.Aims: To assess the habitat characteristics selected by Ferruginous Pygmy Owls during the breeding season.Methods: Random transects were performed across a portion of the Luro Natural Reserve by broadcasting Ferruginous Pygmy Owl calls. Locations along these transects were used to carry out a presence\/absence habitat analysis at micro- and macro-habitat levels.Results: Ferruginous Pygmy Owls were found in the majority of sampled locations. However, their presence was not associated with the vegetation structure and composition around sampled locations. Model outputs suggested similarity among presence and absence locations at both spatial scales, though owls showed a slight preference for areas with older trees and higher edge-density values. Conclusion: The Ferruginous Pygmy Owl was more abundant than previously thought in the Caldén xerophytic forest, emphasizing the habitat?s conservation importance, particularly because of the landuse changes that the La Pampa region is experiencing (e.g. forest exploitation).","palabras_clave":"Ferruginous Pygmy Owl|Glaucidium brasilianum|Habitat selection|Breeding season"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63745,"idioma_id":4,"titulo":"Is floret primordia death triggered by floret development in durum wheat?","resumen":"Survival of floret primordia initiated seems critical for the determination of grain number and yield in wheat, and understanding what determines floret mortality would help in the development of more robust physiological models of yield determination. The growth of the juvenile spikes has been frequently considered the determinant of grain number, implying that floret development would depend on resource availability and that the onset of floret death would be related to spike growth. However, this model has been recently challenged from a study concluding that floret death started when the most advanced floret primordia reached a particular developmental stage. As the few previous studies on this relationship involved photoperiod treatments which affect both floret development and the onset of spike growth, conclusions cannot be considered mechanistic. This comprehensive study analysed in detail floret development in wheat as affected by resource availability (mainly soil nitrogen levels) and found that the onset of floret death may occur when development of the most advanced florets ranged from stages 5 to 9 and that the average and standard deviation of floret developmental stage coinciding with the onset of floret death was not related to the level of availability of resources. These results provide further support to the model relating the onset of floret death with the initiation of active growth of the juvenile spike in which florets are developing.","palabras_clave":"wheat|seed number|floret development|nitrogen"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63746,"idioma_id":4,"titulo":"Floret development and grain setting differences between modern durum wheats under contrasting nitrogen availability","resumen":"Wheat yield depends on the number of grains per square metre, which in turn is related to the number of fertile florets at anthesis. The dynamics of floret generation\/degeneration were studied in contrasting conditions of nitrogen (N) and water availability of modern, well-adapted, durum wheats in order to understand further the bases for grain number determination. Experiments were carried out during the 2008\u20132009 and 2009\u20132010 growing seasons at Lleida (NE Spain). The first experiment involved four cultivars (Claudio, Donduro, Simeto, and Vitron) and two contrasting N availabilities (50 kgN ha\u20131 and 250 kgN ha\u20131; N50 and N250) while experiment 2 included the two cultivars most contrasting in grain setting responsiveness to N in experiment 1, and two levels of N (N50 and N250), under irrigated (IR) and rainfed (RF) conditions. In addition, a detillering treatment was imposed on both cultivars under the IR+N250 condition. The number of fertile florets at anthesis was increased by ~30% in response to N fertilization (averaging across treatments and spikelet positions). The effect of N and water availability was evident on floret developmental rates from the third floret primordium onwards, as these florets in the central spikelets of all genotypes reached the stage of a fertile floret in N250 while in N50 they did not. In this study, clear differences were found between the cultivars in their responsiveness to N by producing more fertile florets at anthesis (through accelerating developmental rates of floret primordia), by increasing the likelihood of particular grains to be set, or by both traits.","palabras_clave":"wheat|flower development|seed number|detillering"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63747,"idioma_id":4,"titulo":"A linear programming approach for adaptive synchronization of  traffic signals","resumen":"As traffic congestion during rush hours is a growing problem for most cities, there is an increasing need for more effective management of traffic signal control and traffic assignment systems. We present here a new adaptive system based on a linear programming model for the signal control problem.The objective is to minimize the total length of the queues of vehicles waiting at each intersection. The model is based on traffic information provided by real-time sensors installed at each intersection. In order to compare the performance of our program with that of the current scheduling designed by the transit office of Buenos Aires city, we sed a traffic simulation system and real traffic flow data of two pilot areas of the city. Preliminary results are very promising.","palabras_clave":"Urban traffic control|Adaptive signal control|Signal timing|Linear Programming"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63748,"idioma_id":4,"titulo":"Grazing effects of the sea urchin Tetrapygus niger and the snail Tegula atra on a rocky shore of central Peru","resumen":"Here we describe habitat use and the grazing effects of the sea  urchin Tetrapygus niger and the gastropod Tegula atra in the low intertidal zone of a rocky shore in central Peru (Ancón bay: 11° 46?S, 77° 12?W), where these two species were documented as coexisting with no evidence of habitat segregation between them. Gut content and isotopic analyses revealed differences between their diets: T. atra consumes mainly microalgae while T. niger consumed also benthic macroalgae. Individual grazing effects were evaluated with inclusion\/exclusion experiments. Tetrapygus niger prevented the colonization of the substrate by sessile organisms, including the dominant mussel Semimytilus algosus, while T. atra reduced the abundance of microalgae and green ephemeral  macroalgae during early succession, but these effects were overwhelmed  by the rapid increase in cover of S. algosus. We suggest that T. niger plays a key role in this low intertidal community. Through directly limiting mussels to monopolize the substratum, T. niger can reduce the diversity of mussel associated species; conversely, through controlling primary space holders, T. niger can also benefit other grazers that live on bare substrate by maintaining a suitable area for feeding or living","palabras_clave":"GRAZING EFFECTS|PERU|ROCKY SHORE|TEGULA ATRA|TETRAPYGUS NIGER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63749,"idioma_id":4,"titulo":"Chronic indeterminate phase of Chagas disease: mitochondrial involvement in infection with two strains","resumen":"Chagasic cardiopathy has become one of the most frequent causes of heart failure and sudden death, as well as one of the most common causes of cardio-embolic stroke in Latin America. The myocyte response to oxidative stress involves the progression of cellular changes, primarily targeting the mitochondria and modifying therefore the energy supply. In this paper we analysed the effect of the infection of mice with 2 different strains of Trypanosoma cruzi (Tulahuen and SGO Z12) in the chronic indeterminate stage (75 days post-infection), upon the structure and function of cardiac mitochondria. The structural results showed that 83% of the mitochondria from the Tulahuen-infected mice presented an increase in their matrix and 91% of the mitochondria from the SGO Z12-infected group showed a reduction in their diameter (P<0·05).  When the Krebs cycle and mitochondrial respiratory chain functionality was analysed through the measurement of the citrate synthase and complexes I to IVactivity, it showed that their activitywas altered in all cases in a similar manner in both infected groups. In this paper we have demonstrated that the chronic indeterminate phase is not \u2018silent\u2019 and that cardiac mitochondria are clearly involved in the genesis and progression to the chronic chagasic cardiopathy when different factors alter the host-parasite equilibrium.","palabras_clave":"chronic|chagas disease|mitochondria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63750,"idioma_id":4,"titulo":"Clomipramine and benznidazole association for the treatment of acute experimental Trypanosoma cruzi infection","resumen":"Alternative strategies are being designed to identify candidates among drugs already available on the market  that could be used in combination to improve the efficacy of Chagas disease treatment. This work evaluates  the effect of the association of clomipramine (CLO) with benznidazole (BZN) for the treatment of experimental  Chagas disease in the acute stage, in Swiss albino mice infected with Trypanosoma cruzi Tulahuen strain.  Infected mice were treated with CLO 5 mg\/kg\/day and BZN 50 and 100 mg\/kg\/day, each separately or together.  Efficacy of the treatment was evaluated through parasitemia, survival, electrocardiography, histopathological  studies, serological and PCR assays at 90 days post-infection (dpi). All treatments significantly  (P b 0.05) reduced mortality and decreased parasitemia. Histopathological analysis of liver and kidneys of  mice treated with CLO and the drug combination showed less injury than mice treated only with BZN. The  lower dose of BZN (50 mg\/kg\/day) combined with CLO showed the same efficacy as the habitual dose of  BZN (100 mg\/kg\/day) combined with CLO. The therapeutic results from the combination of BZN with CLO  presented lesser side effects than the treatment with BZN.  © 2013 Elsevier Ireland Ltd. All rights reserved.","palabras_clave":"american  trypanosomiasis|chemotherapy|antypanosomal drugs|acute chagas disease"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63751,"idioma_id":4,"titulo":"Effects of in vivo exposure to Roundup on immune system of Caiman latirostris","resumen":"The present study aimed to evaluate the effect of Roundup® (RU, glyphosate-based formulation) on some parameters of the immune system and growth of Caiman latirostris. Seventy-two caimans (20-day-old) from Proyecto Yacaré (Gob. Santa Fe\/MUPCN) were used. Two groups were exposed for two months to different concentrations of RU (11 or 21 mg\/L; taking into account the concentration recommended for its application in the field), while one group was maintained as control. The RU concentration was progressively decreased through the exposure period to simulate glyphosate degradation in water. Animals were measured and weighed at the beginning and end of the experiment, and blood samples taken after exposure to determine total and differential white blood cell (WBC) counts as well as total protein concentration (TPC), and for performing protein electrophoresis. The results showed that, compared against control hosts, there was a decrease in WBC counts, a higher percentage of heterophils, a higher TPC (with a low percentage of F2 protein fraction), and a negative effect on growth in the young caimans exposed to RU. These results demonstrate that in vivo exposure to RU induced alterations in the selected immune parameters, plasma proteins, and growth of caimans, thereby providing relevant information about the effects of this type of pesticide in this important species in the Argentinian wetlands","palabras_clave":"BROAD SNOUTED CAIMAN|IMMUNE SYSTEM|PESTICIDES|GLYPHOSATE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63752,"idioma_id":4,"titulo":"The most complete known Neogene Glyptodontidae (Mammalia, Xenarthra, Cingulata) from northern South America: taxonomic, paleobiogeographic and phylogenetic implications","resumen":"The knowledge of northern South American Glyptodontidae (Mammalia, Xenarthra, Cingulata) is very scarce compared with that of southern South American taxa, which have been systematically studied since the Nineteenth Century. Recently, the northern taxa (originally assigned to the Glyptodontidae Propalaehoplophorinae Asterostemma and Propalaehoplophorus) have been reinterpreted as basal Glyptodontinae, belonging to the new genus Boreostemma. In this contribution we present and describe the most complete Neogene Glyptodontidae from northern South America (Middle Miocene of the Honda Group, La Venta, Colombia), and its main taxonomic, paleobiogeographic and phylogenetic implications.  This new material expands the morphological characterization of B. acostae and corroborates differences compared with Glyptodontidae from Miocene southern South America. A cladistic analysis corroborates the monophyly of the Glyptodontinae, being B. acostae and B. venezolensis the sister group of the remaining taxa of Glyptodontinae. The traditionally recognized genera (e.g., Glyptodon and Glyptotherium) constitute natural groups. Whereas the Miocene seems to represent a diversification period for Glyptodontidae in Southern South America, the recorded taxa in northern South America are restricted with certainty only to the Glyptodontinae Boreostemma.","palabras_clave":"GLYPTODONTIDAE|XENARTHRA|NEOGENE|IMPLICATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63753,"idioma_id":4,"titulo":"Multidimensional Poverty in Latin America and the Caribbean. New Evidence from the Gallup World Poll","resumen":"This paper studies poverty in Latin America and the Caribbean from a multidimensional perspective, exploiting the Gallup World Poll, a survey that provides a unique opportunity to perform intercountry comparisons. By applying factor analysis we find that welfare can be appropriately summarized by three dimensions: income, subjective welfare and \u201Cbasic needs\u201D. Another finding is that the US$ 1 line appears to be a reasonable cut-off value to measure food deprivation.","palabras_clave":"POVERTY|LATIN AMERICA|GALLUP WORLD POLL|MULTIDIMENSIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63754,"idioma_id":4,"titulo":"The effect of anthropic pressures and elevation on the large and medium-sized terrestrial mammals of the subtropical mountain forests (Yungas) of NW Argentina","resumen":"We conducted a 55-day long camera-trap survey in the Yungas subtropical forest in NW Argentina, to assess the effect of human accessibility, conservation status of the area, domestic animals and elevation on the diversity and composition of the large and medium-sized native terrestrial mammal assemblage. We deployed 24 camera-trap stations at distances of ~2 km from each other. The study area is covered by continuous forest and has its center in the small community of Acambuco, in the Acambuco Provincial Reserve. The main economic activity in the area is oil\/gas exploitation. Local residents raise cattle, hunt and use timber and non-timber forest products. The human impact was indirectly measured with an accessibility cost model. We used a multiple regression ANCOVA to assess the effect of elevation (range: 628-1170 masl), accessibility, protection status (reserve vs not) and frequency of records of domestic animals on the native mammal species richness and on a nonmetric multidimensional scaling (NMDS) ordination based on the frequency of records of the native mammals recorded at > 3 camera-trap stations. We recorded 15 species of native mammals. Native mammal species richness decreased with elevation. Elevation was correlated with NMDS axes. Other predictive variables had no effect on species richness or the NMDS ordination, probably as a result of the relatively narrow range of conditions assessed in this study. The effect of elevation on mammal assemblages should be considered in landscape planning processes aimed at promoting biodiversity conservation.","palabras_clave":"Accessibility cost model|Biodiversity conservation|Elevation|Hunting|Protected areas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63755,"idioma_id":4,"titulo":"The impact of flood control on the loss of wetlands in Argentina","resumen":"1. Wetlands are among the most important ecosystems on Earth, but historically they have been degraded and destroyed by humans. The south east of Córdoba province in central Argentina was covered by hundreds ofwetlands in a vast matrix of grasslands and savannas. In the last few centuries, this area has been mostly transformed into agriculture, forcing wetlands to become the last refuge for remaining wildlife.2. Since the mid-1970s, a gradual increase in rainfall has enlarged the area occupied by wetlands. To reverse the situation, vast flooded regions were altered by the construction of artificial drainage channels, including importantareas for conservation of biodiversity.3. A non-supervised classification of satellite images was used to assess the changes in flooded areas of south-eastern Córdoba before the main floods (1987\u20131988) and after channelizations occurred (2007). Areas with highchannelization (Córdoba) and non-channelization (Santa Fe) were compared for years with the same amount of accumulated rainfall.4. The pluviometric registers in both regions showed a trend of increasing annual rainfall, and this was reflected in a 65.9% increase (64 837 ha) of the flooded area in Santa Fe. Conversely, the channelized area in Córdobasuffered a loss of 12% of its ponds, corresponding to a 14.7% reduction of the flooded area (11 655 ha). A greater reduction in the flooded area (42.1%) was observed when considering only the western portion of theCórdoba site where most of the channelization occurred.5. These results indicate that besides the mitigation of floods, the channelization in Córdoba favoured agriculture expansion at the expense of wetlands, thereby destroying these wildlife refuges. Wetlands areimportant for their biodiversity, ecosystem services, and cultural legacy. In central Argentina the channelization process still continues, so it is of the utmost importance to support conservation actions leading to sustainablemanagement and territorial planning of this region.","palabras_clave":"CANAL|POND|HABITAT MANAGEMENT|REMOTE SENSING|FLOODING|LAND DRAINAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63756,"idioma_id":4,"titulo":"Detection of antibodies against to brucella abortus, leptospiras spp. and apicomplexa.","resumen":"Water buffalo industry has become a profitable activity worldwide, including the Northeast of Argentina (NEA). However, research on diseases affecting this species is scarce. The aim of the present study was to detect antibodies against Brucella abortus, Leptospira spp., Neospora caninum, Toxoplasma gondii, and Sarcocystis spp. in 500 water buffalo cows from five ranches (100 animals each) in the NEA. Serum samples were tested for B. abortus by fluorescence polarization assay, Leptospira spp. By microagglutination test, and N. caninum, T. gondii, and Sarcocystis spp. by indirect fluorescent antibody tests. Overall, the proportion of seropositive animals was 6.4, 22.2, 42.2, 25.4, and 50.8 % for brucellosis, leptospirosis, neosporosis, toxoplasmosis, and sarcocystosis, respectively. The proportion of seropositive animals for all diseases was statistically different among herds (p<0.05). Statistical differences were also detected among age groups for brucelosis and neosporosis (p<0.05). The detection of specific antibodies to B. abortus, Leptospira spp., and several Apicomplexa protozoans in water  buffaloes in the NEA is reported in this study.","palabras_clave":"BUBALUS BUBALIS|BRUCELLA ABORTUS|LEPTOSPIRA|APICOMPLEXA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63757,"idioma_id":4,"titulo":"A composite beam finite element for multibody dynamics: Application to large wind turbine modeling","resumen":"This work presents a beam finite element with multibody capabilities for modeling high aspect ratio composite wind turbine components, particularly the tower and the blades. The proposed formulation is based on an Updated Lagrangian approach, in which the virtual work equations are written as a function the director field and its derivatives. The cross-sectional modeling of the wind turbine components is based on the constitutive relations obtained from the analysis of the mechanics of composite laminates. The formulation of the equations of motion and the derivation of a hinge joint is presented. Several results for a large wind turbine model are shown.","palabras_clave":"Wind turbines |Total Lagrangian |Anisotropic materials| Multibody systems"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63758,"idioma_id":4,"titulo":"Photoacoustic Detection of Perfluorocarbon Tracers in Air for Application to Leak Detection in Oil-Filled Cables","resumen":"The underground oil-filled cable consists of a hollow copper conductor   surrounded by oiled paper which acts as electrical insulation. The oil   flows along the conductor and diffuses through it to the insulating   paper. A lead sheath is used as the outer retaining wall. As the   deterioration of this cover may cause a loss of insulation fluid, its   detection is very important since this high voltage and power cable is   used in cities even under sidewalks. The method of perfluorocarbon vapor   tracers, based on the injection and subsequent detection of these   volatile chemical substances in the vicinity of the cable, is one of the   most promising methods, so far used in combination with gas   chromatography and mass spectrometry. In this study, the possibility of   detecting two different tracers by means of resonant photoacoustic spectroscopy is studied. The beam from a tunable amplitude-modulated  laser goes through an aluminum cell with quarter wave filters at both   ends of an open resonator and an electret microphone in its center,   attached to the walls. The calibration of the system for either substance diluted in chromatographic air showed a higher sensitivity for it, so the experiment was completed checking the behavior of this substance   in samples prepared with ambient air in order to analyze the   application of the system to field studies  <","palabras_clave":"OIL-FILLED CABLE|PERFLUOROCARBON|PHOTOACOUSTIC|TRACER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63759,"idioma_id":4,"titulo":"TAUTOMERIZABLE STYRENIC COPOLYMERS CONFINED IN AAO TEMPLATES","resumen":"Abstract: The aim of this work is to prepare new keto-enol tautomerizable  polystyrene copolymer nanofibers, by infiltrating the polymer into  nanoporous anodized aluminum oxide (AAO) nanocavities, and to examine the  influence of confinement effects on the polymer properties of  nanostructured polymers in comparison to the bulk. The interest of this  work lies, on the one side, on the presence of tautomeric groups that  could lead to discriminate against specific substrates and, therefore,  could make them interesting in biomedicine. And, on the other hand, on  the topographic characteristic of oriented nanofibrous structures that  could be convenient for cell regeneration.  In this work nanostructured keto-enol tautomerizable polystyrene  copolymer nanofibers, i.e. Styrene-co-2-methyl-3-oxo-5-phenyl-4-  pentenonitrile (St-co-MOP) of different chemical composition, have been  prepared under soft infiltration conditions of the polymer into AAO  templates. For all compositions, SEM images show stacked nanofibers  reproducing the dimensions of AAO nanopores and Raman confocal  spectroscopy of the samples, confirms the homogenous chemical  distribution of nanofibers through all the length of AAO pores. The glass  transition temperature values for St-co-MOP copolymer nanofibers inside  AAO nanocavities determined by differential scanning calorimetry are  higher than those corresponding to the bulk copolymers. From thermograms  of nanofibers obtained by thermo gravimetric analysis (TGA), in an air  atmosphere, the existence of two thermal peaks is observed, the second  one probably caused by the presence of tautomeric forms in the  copolymers. Moreover, the elastics moduli found for the bulk copolymers  are consistent with their employment for building extracellular matrices  In summary, this study clearly demonstrates the feasibility of  producing nanofibers from styrene-co-2-methyl-3-oxo-5-phenyl-4-  pentenonitrile (St-co-MOP) tautomeric polymers with a high degree of  homogeneity and appropriate thermal and mechanical properties.  Furthermore, preliminary results for tautomeric copolymers do not show  cytotoxic effects, indicating that the obtained nanostructured copolymer  combines several relevant properties required for its future applications  as a biomaterial for cell scaffolds.","palabras_clave":"Tautomeric Copolymers|AAO templates|nanostructured scaffolds"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63760,"idioma_id":4,"titulo":"Xiphosurid trackways in a Lower Cretaceous tidal flat in Patagonia: Palaeoecological implications and the involvement of microbial mats in trace-fossil preservation","resumen":"Lower Cretaceous trackways assignable to xiphosurids were recently found in tidaly influenced marginal-marine deposits of the Agrio Formation (Patagonia Argentina). The aim of this paper is to describe these trace fossils in detail, discuss their conditions of preservation, make paleoecological and taphonomic inferences from them, and analyse the importance of their paleogeographic location. These trace fossils are assigned to Kouphichnium, and five track morphotypes are established. Microbially induced sedimentary structures (MISS) are associated with the trackways. Scanning electron microscope (SEM) studies document the involvement of microbial mats in the preservation by the presence of cyanobacteria-like filament sheaths. The studied surface could represent a high-tide mating ground associated with a very shallow water deposits on a warm Cretaceous tidal flat. This is the first convincingly documented trackways produced by xiphosurids in the Early Cretaceous worldwide and the second fossil record of this group form the Cretaceous in the South Hemisphere.","palabras_clave":"XIPHOSURID TRACKWAYS|MARGINAL MARINE|KOUPHICHNIUM|CRETACEOUS|SOUTH AMERICA|MICROBIALLY INDUCED SEDIMENTARY STRUCTURES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63761,"idioma_id":4,"titulo":"Why do trees adjust water relations and hydraulic architecture","resumen":"Studies on water relations, hydraulic architecture and effects of nutrients on growth in trees have been studied for many years in relative isolation. Only recently the functional relations of these groups of plant traits have been investigated with a more integrative approach (e.g. Harvey and van den Driessche, 1999; Clearwater and Meinzer 2001: Ewers et al. 2000, 2001; Bucci et al. 2006; Hacke et al. 2010; Faustino et al. 2013). In this commentary we attempt to answer the question of why water relations and the hydraulic architecture of trees are functionally related with their nutrient availability. We will focus on results of studies where nutrient limitations have been removed or alleviated by additions of the two most important macronutrients: nitrogen and phosphorous.","palabras_clave":"NUTRIENTS|HYDRAULIC ARCHITECTURE|NITROGEN|LEAF WATER POTENTIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63762,"idioma_id":4,"titulo":"The stem xylem of Patagonian shrubs operates far from the point of catastrophic dysfunction and is additionally protected from drought- induced embolism by leaves and roots","resumen":"&nbsp;  Hydraulic architecture was  studied in shrub species differing in rooting depth in a cold desert in  Southern Argentina. All species exhibited strong hydraulic segmentation between  leaves, stems and roots with leaves being the most vulnerable part of the  hydraulic pathway. Two types of safety margins describing the degree of  conservation of the hydraulic integrity were used: the difference between  minimum stem or leaf water potential (Y) and the Y at which stem or leaf hydraulic function was reduced by 50% (Y - Y50), and the difference between leaf and stem Y50. Leaf Y50 - stem Y50 increased  with decreasing rooting depth. Large diurnal decreases in rootspecific hydraulic  conductivity suggested high root vulnerability to embolism across all species.  Although stem Y50 became more  negative with decreasing species-specific Ysoil and minimum stem Y,  leaf Y50 was independent of Y  and minimum leaf Y. Species with embolism-resistant stems also had  higher maximum stem hydraulic conductivity. Safety margins for stems were &gt;2.1 MPa, whereas those for leaves were  negative or only slightly positive. Leaves acted as safety valves to protect  the integrity of the upstream hydraulic pathway, whereas embolism in lateral  roots may help to decouple portions of the plant from the impact of drier soil layers.  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;                                                                                 ","palabras_clave":"HYDRAULIC CONDUCTIVITY|HYDRAULIC SEGMENTATION|LEAF WATER POTENTIAL|SAFETY MARGIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63763,"idioma_id":4,"titulo":"Homeostasis in leaf water potentials on leeward and windward sides of desert shrub crowns: water loss control vs. high hydraulic efficiency","resumen":"Phenotypic plasticity in morphophysiologicalleaf traits in response to wind was studied in two dominantshrub species of the Patagonian steppe, used as modelsystems for understanding effects of high wind speed onleaf water relations and hydraulic properties of smallwoody plants. Morpho-anatomical traits, hydraulic conductanceand conductivity and water relations in leaves ofwind-exposed and protected crown sides were examinedduring the summer with nearly continuous high winds.Although exposed sides of the crowns were subjected tohigher wind speeds and air saturation deficits than theprotected sides, leaves throughout the crown had similarminimum leaf water potential (WL). The two species wereable to maintain homeostasis in minimum WL using differentphysiological mechanisms. Berberis microphyllaavoided a decrease in the minimum WL in the exposed sideof the crown by reducing water loss by stomatal control,loss of cell turgor and low epidermal conductance.Colliguaja integerrima increased leaf water transport efficiencyto maintain transpiration rates without increasingthe driving force for water loss in the wind-exposed crownside. Leaf physiological changes within the crown help toprevent the decrease of minimum WL and thus contribute tothe maintenance of homeostasis, assuring the hydraulicintegrity of the plant under unfavorable conditions. Theresponses of leaf traits that contribute to mechanicalresistance (leaf mass per area and thickness) differed fromthose of large physiological traits by exhibiting low phenotypicplasticity. The results of this study help us tounderstand the unique properties of shrubs which havedifferent hydraulic architecture compared to trees.","palabras_clave":"hydraulic conductance|osmotic potential|patagonia|wind|stomatal conductance"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63764,"idioma_id":4,"titulo":"Water Storage Discharge and Refilling in the Main Stems of Canopy Tree Species Investigated Using Frequency Domain Reflectometry and Point Electronic Dendrometers","resumen":"Trunks of large trees play an important role in whole-plant water balance but technical difficulties have limited most hydraulic research to small stems, leaves and roots. To investigate the dynamics of water economy processes in tree trunks diurnal changes in volumetric water content (VWC) and radius length were monitored continuously using frequency domain moisture sensors and electronic dendrometers respectively, in 10 canopy tree species of a subtropical seasonal forest in Northern Argentina. In addition, species-specific water storage capacitance of the active xylem was assessed using pressure volume relationships determined with a psychometric method. In nearly all the species, trunk VWC and radius length decreased during the daytime when the air saturation deficit increased, suggesting that the trunk water storage was drawn by canopy transpiration, while the increase of VWC during the nighttime suggests stem water storage recharge. Species with higher water storage capacitance exhibited lower wood density, and higher average VWC. ","palabras_clave":"SAPWOOD CAPACITANCE|TROPICAL TREES|FOURIER SERIE TIME|VOLUMETRIC WATER CONTENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63765,"idioma_id":4,"titulo":"Preference for different inorganic-nitrogen forms among plant-functional types and species of the Patagonian steppe","resumen":"            We explored species-specific  preferences for nitrate and ammonium as an alternative niche separation in ecosystems  where nitrogen (N) is mostly inorganic. The Patagonian steppe is dominated by shrubs  and grasses. Shrubs absorb water and nutrients from deep soil layers, which are  N poor, while grasses have the opposite pattern. We hypothesized that shrub and  grass preferences for inorganic-N forms are different, and that the rate of  potential N-uptake is greater in shrubs than in grasses. We hydroponically grew  individuals of six dominant species in solutions with different  ammonium:nitrate concentration ratios. Nitrate uptake was higher in shrubs,  while ammonium uptake was similar between plant-functional types. The NH4+:NO3-  uptake ratio was significantly lower for shrubs than grasses showing opposite  preference for different N forms. Shrubs, which under field conditions have  deeper rooting systems than grasses, showed higher N-absorption capacity than  grasses and preference for the more mobile N-form, nitrate. Grasses preferred  ammonium over nitrate and have lower N-uptake rates than shrubs. These  complementarities between grasses and shrubs suggest a more thorough  exploitation of resources by diverse ecosystems than those dominated by just  one functional type. Loss of one group or a significant change in its abundance  would represent a reduction in resource-use efficiency and ecosystem  functioning.  ","palabras_clave":"N economy|niche partitioning|arid ecosystems|competition"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63766,"idioma_id":4,"titulo":"Fibroblast growth factor receptor 1 ( FGFR1 ) variants and craniofacial variation in Amerindians and related populations","resumen":"AbstractOBJECTIVES:The polymorphic site rs4647905 of the FGFR1 gene was previously associated with a decrease in cephalic index (CI). Here, we evaluate the relationships between genotypes and cephalometric measurements and indices in one Mexican Native and two mestizo Mexican populations using two haplotype-tag SNPs (rs4647905 and rs3213849) that represent >85% of the FGFR1 variability, plus three other SNPs (rs2293971, rs2304000, and rs930828) situated nearby. In addition, we genotyped five South American natives, two European, one African, and one Siberian populations to evaluate their intra and intercontinental population diversity.METHODS:The five SNPs were tested and the craniofacial measurements and indices were collected using standardized procedures. Principal Component Analysis was used to verify individual\/population comparisons. Associations were performed through the generalized linear model (GLM), coefficient of determination R(2) and linear regression tests.RESULTS:We found a tendency for a decrease in CI in individuals homozygous for allele rs4647905C, regardless of the population to which they belong, though the effect is more pronounced in mestizo. When the GLM analyses were performed using the absolute\/linear cephalometric measurements, a statistically significant association was found between four SNPs and head length in the mestizo population.CONCLUSIONS:FGFR1 polymorphisms, especially rs4647905, can have an important role in the normal human skull variation, primarily due to their influence in head length, which would affect other cephalometric absolute\/linear measures as well as indices like CI as a result of the pervasive nature of the morphological integration that characterizes the human skull.","palabras_clave":"FIBROBLAST GROWTH FACTOR RECEPTOR GENE|RANIOFACIAL DEVELOPMENT|NATIVE AMERICANS|MEXICAN POPULATIONS|CEPHALIC INDEX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63767,"idioma_id":4,"titulo":"Exploiting electron storage in TiO2 nanoparticles for dark reduction of As(V) by accumulated electrons","resumen":"UV irradiation of an ethanolic sol of TiO2 nanoparticles produces a trapped Ti(III) centre and a noticeable Burstein shift. Direct evidence that the accumulated charges can drive the otherwise forbidden reduction of As(V) by conduction band electrons is presented.","palabras_clave":"UV IRRADIATION|ARSENIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63768,"idioma_id":4,"titulo":"A comparative assessment of linearization methods for bilinear models","resumen":"In this article, optimization problems with bilinear constraints involving one discrete variable are studied. Several industrial problems present bilinear non-convex constraints which are dif&#64257;cult to solve to  global optimality. For this purpose models must be reformulated what in general terms increases the  problem size. This article proposes two disjunctive transformation techniques which are compared to  other approaches presented in the literature. An analysis is made comparing qualitative and quantitative characteristics of the methods employed. In order to implement proposed transformations, three  industrial cases are studied: trim-loss in a paper mill, cutting stock in the production of carton board  boxes and the purchase, inventory and delivery optimization problem. All of them are reformulated and  solved using the strategies included in the paper. Several instances of each problem are evaluated and  their results are analyzed comparing performance of the different methods.","palabras_clave":"MINLP |LINEARIZATION METHODS|BILINEAR TERMS|DISCRETE DECISIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63769,"idioma_id":4,"titulo":"A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop.","resumen":"Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1-miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17&#8764;92 and miR-106a&#8764;363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17&#8764;92 \/ miR-106a&#8764;363 miRNAs in controlling NSC proliferation and neuronal differentiation.","palabras_clave":"Transcripción|Células madre neurales|E2F1|microARNs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63770,"idioma_id":4,"titulo":"Acute effects of pregabalin  on the function and cellular distribution of Ca(V)2.1 in HEK293t cells","resumen":"We established a cell model to study the acute effects of pregabalin (PGB), a drug widely used in epilepsy  and neuropathic pain, on voltage gated CaV2.1 (P\/Q-type) calcium channels function and distribution at  the membrane level. HEK293t cells were transfected with plasmids coding for all subunits of the CaV2.1  channel. We used a 1 fused to an eGFP tag to follow its distribution in time and at different experimental  conditions.  The expressed channel was functional as shown by the presence of barium-mediated, calcium currents  of transfected cells measured by \u2018whole-cell voltage-clamp\u2019 recordings, showing a maximum current  peak in the I\u2013V curve at +20 mV. The GFP fluorescent signal was confined to the periphery of the cells.  Incubation with 500 M PGB, that binds 2 subunits, for 30 min induced changes in localization of the  fluorescent subunits as measured by fluorescent time lapse microscopy. These changes correlated with  a reversible reduction of barium currents through CaV2.1 calcium channels under the same conditions.  However, no changes in the cellular distribution of the subunits were visualized for cells either expressing  another membrane associated protein or after exposure of the CaV2.1 channels to isoleucine, another 2  ligand. Together these results show strong evidence for an acute effect of PGB on CaV2.1 calcium channels\u2019  currents and distribution and suggest that internalization of CaV2.1 channels might be a mechanism of  PGB action.","palabras_clave":"Pregabalin|CaV2.1 calcium channels|Neuropathic pain|Epilepsy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63771,"idioma_id":5,"titulo":"Una reconceptualización del concepto de elemento como base para una nueva  representación del sistema periódico","resumen":"    ABSTRACT (A reconceptualization of  the concept of element as the basis for a new    The aim of this paper is to propose a  new conceptualization of the term \u2018element\u2019 as the basis    for a new representation of the periodic  system. For this purpose we begin by recalling the dual    sense of the concept of element. Next,  we develop the \u2018limits isotopes\u2019 argument which is the    basis of the new periodic chart. This  task leads us both to reconceptualize the notion of element    and to characterize the term \u2018basic  substance\u2019. In turn, the argument is used to face the    epistemological problem with hydrogen  and helium in the periodic table. Finally, the Döbereiner\u2019s    triads are used to calculate atomic  masses in three periodic charts: the medium-long-form, the    modified \u2018left-step\u2019 proposed by Scerri,  and the proposed in this work. Evaluation results allows    us to stand out the fruitful predictive  power of our periodic system.  ","palabras_clave":"new periodic chart|element|isotopes|hydrogen"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63772,"idioma_id":4,"titulo":"Pore Size Distribution of Ordered Nanostructured Carbon CMK-3 by means of Experimental Techniques and Monte Carlo Simulations","resumen":"The design and study of new materials with specific properties is interesting in several scientific and technological fields. In the last years, nanostructured carbons (NC) have rapidly attracted the attentionof some researchers due to their physicochemical properties useful for many applications among them in adsorption and catalysis.In this work, the synthesis of a NC was carried out by a nanocasting method, using as a template a very ordered mesoporous material (SBA-15) and sucrose as carbon source. The final material consists of an ordered arrangement of parallel carbon nanorods bonded with some carbon nanowires (CMK-3 type), formed in the mesopores and micropores of the inorganic matrix. The inorganic matrix and the NC were structurally characterized by X-ray diffraction, texturally by N2 sorption at 77 K, and morphologically by scanning electron microscopy. The Nitrogen experimental isotherms were simulated using Grand Canonical Monte Carlo (GCMC) method based on two kernels using slit and cylindrical pore  models. From these models were obtained the pore size distributions (PSD) which were compared with those obtained by Quenched Solid Density Functional Theory (QSDFT) model. The GCMC simulation showed a good agreement with experimental adsorption isotherms and some differences with the corresponding pore size distribution obtained by Density Functional Theories Methods. These correlations validate the presentedGCMC method as an alternative to study in detail the porosity of these materials.","palabras_clave":"Nanostructured|carbon|PSG|GCMC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63773,"idioma_id":4,"titulo":"Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of  barley aleurone cells.","resumen":"Phosphatidic acid (PA) is the common lipid product in abscisic acid (ABA) and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid) rapidly (minutes) in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate  phosphatase 1 and 2 isoforms (E.C. 3.1.3.4) was verified in isolated aleurone membranes in vitro, the former but not the latter being Specifically responsive to the presence of GA or ABA. The in vitro DGPP  phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase e that allows the production of 18:2-DGPP from 18:2-PA e is stimulated by ABA. The long term effects (24 h) of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the  substrate of PAP1 and\/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment.  The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubation","palabras_clave":"ALEURONA|ABSCISIC ACID|DIACYLGLYCEROL PYROSPHOSPHATE|GIBBERELLYC ACID"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63774,"idioma_id":4,"titulo":"Understanding the Lévy ratchets in terms of Lévy jumps","resumen":"We investigate the overdamped dynamics of a particle in a spatiallyperiodic potential with broken reflection symmetry and subject to the action of a symmetric white Lévy noise. The system (referred to as the Lévy ratchet) has been previously studied using both Langevin and fractional Fokker\u2013Planck formalisms, with the main find being the existence of a preferred direction of motion toward the steepest slope of the potential, producing a non-vanishing current. In this contribution we develop a semi-analytical study combining the Fokker\u2013Planck and Langevin formalisms to explore the role of Lévy flights on the system dynamics. We analyze the departure positions of Lévy jumps that take theparticle out of a potential well as well as the rates and lengths of such jumps, and we study the way in which long jumps determine the non-vanishing current. We also discuss the essential difference from the Gaussian-noise case (producing no current). Finally we study the current for different potential shapes as a function of the amplitude of the potential barrier. In particular, we show that standard Lévy ratchets produce a non-vanishing current in the infinite-barrier limit. This latter counterintuitive result can be easily understood in terms of the long Lévyjumps and analytically demonstrated.","palabras_clave":"Ratchets|Levy noise|Molecular motors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63775,"idioma_id":4,"titulo":"Directed transport induced by &#945;-stable Lévy noises in weakly asymmetric periodic potentials","resumen":"We study the motion of a particle in spatially periodic potentials with broken mirror symmetry under the influence of white &#945;-stable Lévy noises. We consider both time-independent and fluctuating potentials. We focus on cases in which the spatial asymmetry of the potential is due not to a difference between the distances from an absolute minimum to the absolute maximum on its left and to the absolute maximum on its right but only to the curvatures of the potential profiles. The analysis is performed using the fractional Fokker-Planck formalism. Consistent results from Langevin simulations are also presented. We analyze the influence of the symmetry properties of the potentials in combination with the fluctuating characteristics of the system in the determination of the current. We find different situations in which both the absolute value and the direction of the current depend not only on the properties of the potential but also on the parameters characterizing the &#945;-stable Lévynoise. Among other features, we analyze the case of supersymmetric potentials. In particular, we show that a static supersymmetric potential produces no current, and we analyze the conditions for observing a nonvanishing current when the potential fluctuates between different supersymmetric profiles.","palabras_clave":"Molecular motors|Ratchets|Levy noise"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63776,"idioma_id":4,"titulo":"Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution","resumen":"The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg \/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p &lt; 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p &lt; 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 andIGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p &lt; 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability.","palabras_clave":"PANAX GINSENG|BOVINE MAMMARY GLAND|INVOLUTION|IGF"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63777,"idioma_id":4,"titulo":"Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease","resumen":"Cystic  ovarian disease (COD) is one of the main factors responsible for reproductive  disorders in cattle. Although the pathogenesis and mechanism of cyst formation  are not fully understood, it  has been proposed that the IGF system could play an essential role, as it is a  key intraovarian regulator. The  aim of the present study was to determine whether the altered levels in IGF1  detected in bovines with COD are associated with changes at mRNA level or with  differential modulation by IGFBPs. The mRNA levels of the IGF components studied  were analyzed by real time PCR and in situ hybridization, and IGFBP expression  and activity were assayed by immunohistochemistry and ligand blot respectively.  Results showed a decreased IGF1 mRNA  level due to a lower granulosa cell gene expression in cystic follicles (P &lt;  0.05). Results also showed variations in IGFBP expression in the intraovarian  cellular compartment and concentration in follicular fluid, and suggest that IGFBP3  is a key regulator of intrafollicular IGF1 in animals with COD.","palabras_clave":"CATTLE|CYSTIC OVARIAN DISEASE|INSULIN-LIKE GROWTH FACTOR|OVARIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63778,"idioma_id":4,"titulo":"Inter-annual and inter-decadal variability of dry days in Argentina","resumen":"This work proposes to employ the number of dry days (days without precipitation) as a variable of study, and to analyse their spatial and temporal variability in Argentina. Climatological aspects of dry days, such as their annual mean values and its seasonal cycle, were discussed and compared with precipitation features in the country. Linear trends in the annual number of dry days (ANDD) were identified for the period 1960?2005. Most of the regions exhibited decreasing trends, but few stations showed significant ones. The most important trends were present in the Central-West region and over the Patagonian coast and their magnitudes indicated a decrease of two to six dry days per decade. These trends coincide with the observed increase of accumulated precipitation in part of the country during the second half of the 20th century. To identify long-term fluctuations in the ANDD, a low pass filter, a wavelet analysis and a cubic polynomial fit was applied to the longest time series of the selected locations. Through these methodologies, periodicities were identified in the inter-annual and inter-decadal ranges for the last 80 years. The most important significant cycles were observed in the range of 10?24 years, but also, depending on the region, significant inter-annual variations were found.","palabras_clave":"DRY DAYS|CLIMATOLOGY|LINEAR TRENDS|WAVELET ANALYSIS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63779,"idioma_id":4,"titulo":"Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth","resumen":"Human embryonic stem cells (hESCs) are self-renewing pluripotent cells that can differentiate into specialized cells and hold great promise as models for human development and disease studies, cell-replacement therapies, drug discovery and in vitro cytotoxicity tests. The culture and differentiation of these cells are both complex and expensive, so it is essential to extreme aseptic conditions. hESCs are susceptible to Mycoplasma sp. infection, which is hard to detect and alters stem cell-associated properties. The purpose of this work was to evaluate the efficacy and cytotoxic effect of Plasmocin(TM) and ciprofloxacin (specific antibiotics used for Mycoplasma sp. eradication) on hESCs. Mycoplasma sp. infected HUES-5 884 (H5 884, stable hESCs H5-brachyury promoter-GFP line) cells were effectively cured with a 14 days Plasmocin(TM) 25 µg\/ml treatment (curative treatment) while maintaining stemness characteristic features. Furthermore, cured H5 884 cells exhibit the same karyotype as the parental H5 line and expressed GFP, through up-regulation of brachyury promoter, at day 4 of differentiation onset. Moreover, H5 cells treated with ciprofloxacin 10 µg\/ml for 14 days (mimic of curative treatment) and H5 and WA09 (H9) hESCs treated with Plasmocin(TM) 5 µg\/ml (prophylactic treatment) for 5 passages retained hESCs features, as judged by the expression of stemness-related genes (TRA1-60, TRA1-81, SSEA-4, Oct-4, Nanog) at mRNA and protein levels. In addition, the presence of specific markers of the three germ layers (brachyury, Nkx2.5 and cTnT: mesoderm; AFP: endoderm; nestin and Pax-6: ectoderm) was verified in in vitro differentiated antibiotic-treated hESCs. In conclusion, we found that Plasmocin(TM) and ciprofloxacin do not affect hESCs stemness and pluripotency nor cell viability. However, curative treatments slightly diminished cell growth rate. This cytotoxic effect was reversible as cells regained normal growth rate upon antibiotic withdrawal.","palabras_clave":"mycoplasma |celulas madre"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63780,"idioma_id":4,"titulo":"Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina","resumen":"Abstract: To detect and characterize Chlamydia trachomatis (C. trachomatis) genotypes in infertile patients of Córdoba, Argentina; 660 endocervical and urethral swabs and semen samples were collected from infertile patients for detection of C. trachomatis by omp A gene with Hemi Nested-PCR and cryptic plasmid-PCR. Sequencing methods of omp A gene were used to identify C. trachomatis genotypes. The sequences obtained were aligned with chlamydial sequences currently available in the GenBank, for the design of the phylogenetic tree. The prevalence of C. trachomatis was 7.27% (48\/660). We did not detect C. trachomatis cryptic plasmid free strains. According to the results of nucleotide sequences, the distribution of genotypes was L1 (50 %) followed by G (25 %), E (12.5%) and D (12.5%). Patients who tested positive to genotype L1 had no symptoms of lymphogranuloma venereum (LGV). This is the first study that provides information about the distribution of C. trachomatis genotypes and the circulation of cryptic plasmid negative strains of C. trachomatis among patients with infertility in Córdoba, Argentina.","palabras_clave":"CLAMIDIA|GENOTYPE|INFERTILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63781,"idioma_id":5,"titulo":"Esquema Perturbativo de las Ecuaciones Conformes de Einstein en el Formalismo de Superficies Nulas","resumen":"          El objetivo de este trabajo es estudiar las formulaciones  KNT y de Nurowski de las ecuaciones conformes de Einstein de vacío a través de un  esquema perturbativo. Luego, se traducen al Formalismo de Superficies Nulas y  se comparan los resultados entre sí.  ","palabras_clave":"TRANSFORMACIONES CONFORMES|ESPACIOS DE EINSTEIN|FORMALISMO DE SUPERFICIES NULAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63782,"idioma_id":4,"titulo":"Future changes in drought characteristics over Southern South America projected by a CMIP5 ensemble","resumen":"The impact of climate change on drought main characteristics was assessed over Southern South America. This wasdone through the precipitation outputs from a multi-model ensemble of 15 climate models of the Coupled Model IntercomparisonProject Phase 5 (CMIP5). The Standardized Precipitation Index was used as a drought indicator, given itstemporal flexibility and simplicity. Changes in drought characteristics were identified by the difference for early(2011-2040) and late (2071-2100) 21st century values with respect to the 1979-2008 baseline. In order to evaluate themulti-model outputs, model biases where identified through a comparison with the drought characteristics from theGlobal Precipitation Climatology Centre database for the baseline period. Future climate projections under moderateand high-emission scenarios showed that the occurrence of short-term and long-term droughts will be more frequent inthe 21st century, with shorter durations and greater severities over much of the study area. These changes in droughtcharacteristics are independent on the scenario considered, since no significant differences were observed on droughtchanges. The future changes scenario might be even more dramatic, taking into account that in most of the region themulti-model ensemble tends to produce less number of droughts, with higher duration and lower severity. Therefore,drought contingency plans should take these results into account in order to alleviate future water shortages that canhave significant economic losses in the agricultural and water resources sectors of Southern South America.","palabras_clave":"CMIP5 MODELS|DROUGHT|CLIMATE CHANGE|SOUTHERN SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63783,"idioma_id":4,"titulo":"Surface circulation types and daily maximum and minimum temperatures in southern La Plata Basin","resumen":"La Plata Basin is one of the most important agriculture and hydropower producingregions worldwide. Extreme climate events such as cold and heat waves and frostevents have a significant socio-economic impact. This work analyzes the influence ofthe surface circulation in Southern South America on daily maximum (TMAX) andminimum (TMIN) temperatures in southern La Plata Basin. A Z test for the comparisonof mean values and a Kolmogorov-Simirnov test for the comparison of distributions ofTMAX and TMIN associated with each circulation pattern were performed. Specificdaily surface circulation types are found to contribute to TMAX and TMIN anomaliesand have a predominant occurrence in the development of the extreme temperatureevents in the region. The TMAX spatial response to the regional low level circulation ismore homogenous and extended than the response of TMIN.","palabras_clave":"ATMOSPHERIC CIRCULATION|SYNOPTIC CLIMATOLOGY|SURFACE TEMPERATURE|CLASSIFICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63784,"idioma_id":4,"titulo":"Stratification of the Radiation Field Inside a Photobioreactor During Microalgae Growth","resumen":"Light availability is a main issue in autotrophic growth of photosynthetic microorganisms. The change of the suspended cells concentration and that of their chlorophylls content during microalgal growth alters the optical properties of the aqueous suspension. This brings about changes in the properties of the radiation field inside the reactor. In this work, we have computed the evolution in time of the local volumetric rate of absorption of photons inside a photobioreactor by means of a Monte Carlo simulation algorithm previously developed. From this study, we have computed two operational variables that are useful tools for the analysis, performance comparison, optimization and scaling up of photobioreactors: the average rate of photon absorption and the volumetric distribution function of photons absorption rates. Based on these two variables, it is possible to systematically quantify the degree of stratification of the culture medium, which is a decisive aspect that hampers the reactor efficiency regarding the energy usage for biomass production.","palabras_clave":"microalgas|fotobioreactores|biodiesel|campo radinate"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63785,"idioma_id":4,"titulo":"Effects of dietary composition on life span of Drosophila buzzatii and its short-lived sibling species D. koepferae","resumen":"Two sibling Drosophila species dramaticallydivergent in longevity, Drosophila buzzatii andDrosophila species dramaticallydivergent in longevity, Drosophila buzzatii andDrosophila buzzatii andD. koepferae, were examined for possible effects ofboth developmental culture medium and dietarycomposition (DC) on longevity. Longevity wasgreatly increased in the longer lived D. buzzatii whenflies were reared and fed on a rich-in-nutrient andcactus-based culture (R-CBC) as compared to longevityin a poor nutrient culture (PNC). In D. buzzatii,life span was further increased by exposing flies toshort periods of a poor-in-nutrient and cactus-basedculture (P-CBC). In contrast, variation in the here usednutrient composition did not change life span in theshorter lived D. koepferae, as longevity in this speciesdid not differ among R-CBC, P-CBC and PNCcultures. Hormesis is a plausible explanation for thebeneficial biological effects against aging arisingfrom brief exposure to a lowed calorie food sourcein D. buzzatii. This study shows that genetic variationbetween closely related species is substantial fordietary effects on longevity., were examined for possible effects ofboth developmental culture medium and dietarycomposition (DC) on longevity. Longevity wasgreatly increased in the longer lived D. buzzatii whenflies were reared and fed on a rich-in-nutrient andcactus-based culture (R-CBC) as compared to longevityin a poor nutrient culture (PNC). In D. buzzatii,life span was further increased by exposing flies toshort periods of a poor-in-nutrient and cactus-basedculture (P-CBC). In contrast, variation in the here usednutrient composition did not change life span in theshorter lived D. koepferae, as longevity in this speciesdid not differ among R-CBC, P-CBC and PNCcultures. Hormesis is a plausible explanation for thebeneficial biological effects against aging arisingfrom brief exposure to a lowed calorie food sourcein D. buzzatii. This study shows that genetic variationbetween closely related species is substantial fordietary effects on longevity.D. buzzatii whenflies were reared and fed on a rich-in-nutrient andcactus-based culture (R-CBC) as compared to longevityin a poor nutrient culture (PNC). In D. buzzatii,life span was further increased by exposing flies toshort periods of a poor-in-nutrient and cactus-basedculture (P-CBC). In contrast, variation in the here usednutrient composition did not change life span in theshorter lived D. koepferae, as longevity in this speciesdid not differ among R-CBC, P-CBC and PNCcultures. Hormesis is a plausible explanation for thebeneficial biological effects against aging arisingfrom brief exposure to a lowed calorie food sourcein D. buzzatii. This study shows that genetic variationbetween closely related species is substantial fordietary effects on longevity.D. buzzatii,life span was further increased by exposing flies toshort periods of a poor-in-nutrient and cactus-basedculture (P-CBC). In contrast, variation in the here usednutrient composition did not change life span in theshorter lived D. koepferae, as longevity in this speciesdid not differ among R-CBC, P-CBC and PNCcultures. Hormesis is a plausible explanation for thebeneficial biological effects against aging arisingfrom brief exposure to a lowed calorie food sourcein D. buzzatii. This study shows that genetic variationbetween closely related species is substantial fordietary effects on longevity.D. koepferae, as longevity in this speciesdid not differ among R-CBC, P-CBC and PNCcultures. Hormesis is a plausible explanation for thebeneficial biological effects against aging arisingfrom brief exposure to a lowed calorie food sourcein D. buzzatii. This study shows that genetic variationbetween closely related species is substantial fordietary effects on longevity.D. buzzatii. This study shows that genetic variationbetween closely related species is substantial fordietary effects on longevity.","palabras_clave":"DIETARY RESTRICTION|LIFE SPAN EXTENSION|ENVIRONMENTAL STRESS|HORMESIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63786,"idioma_id":4,"titulo":"Mitochondrial reactive oxygen species (ROS) as signaling molecules of intracellular pathways triggered by the cardiac renin-angiotensin II-aldosterone system (RAAS).","resumen":"Mitochondriarepresentmajorsourcesofbasalreactiveoxygenspecies(ROS)productionofthecardiomyocyte.TheroleofROSassignalingmoleculesthatmediatedifferentintracellularpathwayshasgainedincreasinginterestamongphysiologistsinthelastyears.Inourlab,wehavebeenstudyingtheparticipationofmitochondrialROSintheintracellularpathwaystriggeredbytherenin-angiotensinII-aldosteronesystem(RAAS)inthemyocardiumduringthepastfewyears.WehavedemonstratedthatacuteactivationofcardiacRAASinducesmitochondrialATP-dependentpotassiumchannel(mitoKATP)openingwiththeconsequentenhancedproductionofmitochondrialROS.Theseoxidantmolecules,inturn,activatemembranetransporters,assodium\/hydrogenexchanger(NHE-1)andsodium\/bicarbonatecotransporter(NBC)viathestimulationoftheROS-sensitiveMAPKcascade.Thestimulationofsucheffectorsleadstoanincreaseincardiaccontractility.Inaddition,itisfeasibletosuggestthatasustainedenhancedproductionofmitochondrialROSinducedbychroniccardiacRAAS,andhence,chronicNHE-1andNBCstimulation,wouldalsoresultinthedevelopmentofcardiachypertrophy.","palabras_clave":"cardiac myocytes|reactive oxigen species|sodium-hydrogenantiporter|secondmessengersystems"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63787,"idioma_id":4,"titulo":"Heavy metal resistant strains are widespread along Streptomyces phylogeny","resumen":"The genus Streptomyces comprises a group of bacteria species with high economic importance. Several of these species are employed at industrial scale for the production of useful compounds. Other characteristic found in different strains within this genus is their capability to tolerate high level of substances toxic for humans, heavy metals among them. Although several studies have been conducted in different species of the genus in order to disentangle the mechanisms associated to heavy metal resistance, little is known about how they have evolved along Streptomyces phylogeny. In this study we built the largest Streptomyces phylogeny generated up to date comprising six genes, 113 species of Streptomyces and 27 outgroups. The parsimony-based phylogenetic analysis indicated that i) Streptomyces is monophyletic ii) It appears as sister clade of a group formed by Kitasatospora and  Streptacidiphilus species, both genera also monophyletic. Streptomyces strains resistant to heavy metals are not confined to a single lineage but widespread along Streptomyces phylogeny. Our result in combination with genomic, physiological and biochemical data suggest that the resistance to heavy metals originated several times and by different mechanisms in Streptomyces history.","palabras_clave":"STREPTOMYCES|HEAVY METALS|RESISTANCE|MOLECULAR PHYLOGENY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63788,"idioma_id":4,"titulo":"Optimal Design of a Corrugated-Wall Photocatalytic Reactor Using Efficiencies in Series and Computational Fluid Dynamics (CFD) Modeling","resumen":"The optimization of a corrugated wall photocatalytic reactor for air treatment was addressed using the concept of  efficiencies in series. A new concept has been introduced: the inner incidence efficiency, associated with the internal configuration of the reactor (for instance, the folding angle in the considered case). The goal of the study was to obtain an optimum for the folding angle, subjected to certain constraints: fixed reactor volume, radiation flux, and gas flow rate. Previous experimentally determined degradation kinetics of a gaseous pollutant was used in the reactor modeling. The simulations of the reactor were performed computationally by using a computational fluid dynamics (CFD) package. The results showed opposed behaviors for the relative catalytic area per unit window area and the relative incident flux with the folding angle. Given that the inner incidence efficiency comprises the product of the two mentioned variables, an optimal folding angle was identified.","palabras_clave":"photoreactors|efficiencies|modelling|air polution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63789,"idioma_id":4,"titulo":"Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms","resumen":"Chromate-resistant microorganisms able to reduce toxic Cr(VI) into insoluble Cr(III) are seen as promising candidates for alleviating Cr(VI) contamination. In this study, chromate-reducing yeasts could be isolated from a textile-dye effluent and associated biofilm by using microcosm methodology with periodical 1 mM Cr(VI)-pulses. Viable cell count seemed to reveal a progressive tolerance increase to Cr(VI). However, fungal colony numbers decreased after 108 h of cultivation most likely as a consequence of the accumulated toxic effects of metal during enrichment. From 49 different Cr(VI)-tolerant fungal morphotypes isolated under selective conditions, 12 yeasts showed resistance up to 50 mM and 6 filamentous fungi up to 2 mM. These highly tolerant yeasts could be subsequently grouped into 8 OTUs (Operational Taxonomic Units) according to the ITS1-NL4 RFLP (Restriction Fragment Length Polymorphism) analysis. From them, microsatellite amplification, sequencing and Cr(VI)-removal ability allowed to select two representative isolates. A polyphasic approach including morphological, physiological\/biochemical characterization and molecular taxonomy analysis allowed to identify these isolates as Cyberlindnera jadinii M9 (previously Pichia jadinii) and Wickerhamomyces anomalus M10 (previously Pichia anomala). Cy. jadinii M9 and W. anomalus M10 were grown in YNB? medium plus 1 mM Cr(VI) at 25 C and pH 5.0, causing complete chromium removal before reaching 48 h of cultivation. Flame Atomic Absorption Spectroscopy (FAAS) assays suggested that Cr(VI) withdrawal was coupled to Cr(III) appearance. Electron microscopy studies indicated absence of precipitates on the cell wall region or microprecipitates into the cellular cytoplasm. These complementary results revealed that biospeciation to Cr(III) would the main detoxification mechanism in selected chromate-resistant yeasts, which could be thus envisaged as promising tools for future biological treatment of Cr(VI) pollution.","palabras_clave":"Textile-dye|chromate-reducing yeasts|polluted waters|microcosms"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63790,"idioma_id":4,"titulo":"Microstructural and electrical characterizations of tungsten-doped La2Mo2O9 prepared by spray pyrolysis","resumen":"La2Mo2-xWxO9 (x=0, 0.5, 1.0, and 1.3) nanocrystallinepowders were synthesized by spray pyrolysis(SP) assisted by an ultrasonic atomizer. Microstructure,sinterability and thermal stability of the prepared powderswere investigated. Spherical particles of 430 to 530 nm indiameter and crystallite sizes in the 30-44 nm range wereobtained. Additionally, the fine microstructure has allowedthe reduction of sintering temperatures with respect to thatusually reported for a solid-state reaction (SSR) method.Pellets with a variety of average grain sizes and morphologies were obtained by applying different sintering programs starting from La2Mo2-xWxO9 powders (x=0 and 1.3) obtained by SP and SSR processes. The electrical properties of these ionic conductors were analyzed by Electrochemical Impedance Spectroscopy (EIS) and correlated with microstructural observations. No significant variation of the electrical properties of these ionic conductors wasfound as compared to conventional ceramics with remarkable microstructural differences.","palabras_clave":"SOFC|LAMOX|Spray pyrolysis|Spark plasma sintering"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63791,"idioma_id":4,"titulo":"A  disappearing  oasis  in  the  semi-arid  Chaco:  Deficient  palm  regeneration  and establishment","resumen":"Palm forests of Copernicia alba are a rare habitat in the semi-arid Chaco of Northwestern Argentina, are centres of high species diversity, and provide key resources for many species. Our goal was to assess the conservation status of five C. alba patches in Northwestern Argentina: Reserve; Embarcación; Palma Sola; Vinalito; and, Talar. We compared patches to identify the sites with greatest conservation needs based on four criteria: population size structure; palm density (of individuals with height >1.30 m); probability of seedlings being browsed; and, presence of potential seed dispersers. We found that three (Embarcación, Reserve, and Talar) out of five sites had palm densities greater than 200 individuals\/ha and only one site (Embarcación) showed a reverse J-shaped size structure for height. Reserve and Embarcación had the greatest probability of seedlings being browsed (0.99 ± 0.01 and 0.88 ± 0.12, respectively). A total of 14 potential disperser species of mammals and birds were recorded across the five sites. Only Reserve harboured all of the potential dispersers, but at least two potential disperser species were recorded at the other sites. None of the palm patches studied had an adequate conservation status. However, Embarcación met three out of four criteria, and therefore it can be considered to have the best conservation status in the semi-arid Chaco. Palma Sola and Vinalito have the greatest conservation needs. To conserve C. alba in Northwestern Argentina, strategies are needed that ensure seedling establishment for future populations to reach an adequate density and structure.","palabras_clave":"BROWSING|FAUNA|LIVESTOCK|PALMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63792,"idioma_id":4,"titulo":"'Improving Test Generation under Rich Contracts by Tight Bounds and Incremental SAT Solving","resumen":"we present a novel and general technique for automated test generation that combines tight   bounds with incremental SAT solving. The proposed technique uses incremental SAT to build test suites targeting a specific testing criterion, amongst various black-box and white-box criteria. As our experimental results show, the combination of tight bounds with incremental SAT, and the testing criterion driven approach implemented in our prototype tool FAJITA, enable us to effectively generate test suites for container classes with rich contracts, more efficiently than other state-of-the-art tools.","palabras_clave":"automated testing|SAT solving|white-box criteria|rich contracts"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63793,"idioma_id":4,"titulo":"Post-transplant lymphoproliferative disorder in adult liver transplant recipients: a South American multicenter experience","resumen":"Post-transplant lymphoproliferative disorder (PTLD) is a major and potentially life-threatening complication after solid-organ transplantation. The aim of this study was to describe the disease characteristics, clinical practices, and survival related to PTLD in adult orthotopic liver transplant (OLT) recipients in South America. We conducted a survey at four different transplant groups from Argentina, Brazil, and Chile. Among 1621 OLT recipients, 27 developed PTLD (1.7%); the mean age at diagnosis was 53.7 (± 14) yr with a mean time of 39.7 (± 35.2) months from OLT to PTLD diagnosis. Initial therapy included reduction in immunosuppression alone in 23.1% of the patients. Either rituximab or chemotherapy was employed as initial or second-line therapy in 76.9% of the patients. PTLD location was frequently extranodal (80.7%) and mostly involving the transplanted liver (59.3%). The overall survival at one and five yr post-PTLD diagnosis was 53.8% and 46.2%, respectively. Significant univariate risk factors for post-PTLD mortality included lactate dehydrogenase &#8805; 250 U\/L (HR 9.66, p = 0.02), stage III\/IV PTLD (HR 5.34, p = 0.004), and HCV infection (HR 7.68, p = 0.01). In conclusion, PTLD in OLT adult recipients is predominantly extranodal, and although mortality is high, long-term survival is possible","palabras_clave":"Post-transplant|ymphoproliferative|life-threatening"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63794,"idioma_id":4,"titulo":"Use of liver grafts from anti-hepatitis B core-positive donors: a multicenter study in Argentina","resumen":"Liver transplantation success is limited by the availability of donors. To overcome this limitation, anti-core-positive donors are increasingly being accepted, but underutilization of this resource still occurs. We performed the current study to determine the prevalence of anti-core-positive donors in our region and to describe the management of these donors and their recipients. Between January 2005 and July 2011, the national transplant database included 2,262 registered liver donors among whom 106 (4.7%) were anti-core-positive including 59 (56%) discarded and 47 (44%) implanted organs. A median of 14.5 offers (range 4-60) were rejected before harvesting and implanting the accepted grafts. The only difference between the implanted and the discarded grafts was found for the alanine aminotransferase level, which was higher among the discarded ones (50 ± 59 UI\/L vs 25 ± 16, P < .05). Among 40 recipients included in the study, 5 (12.5%) did not receive any prophylaxis; 18 (45%) a nucleos(t)ide analog 11 (25.5%), heptitis B immunoglobulin and nucleos(t)ide analogs and 6 (15%) pretransplant hepatitis B vaccination. Over a mean follow-up of 871 ± 585 days, 4 de novo hepatitis B cases were identified at 545, 720, 748, and 1,080 days posttransplantation. None of these patients had received any prophylaxis. In all cases entecavir successfully controlled viral replication. We believe that better utilization of these donors and careful management of their recipients represent safe strategies to expand the liver donor pool in Argentina.","palabras_clave":"LIVER TRANSPLANTATION |ANTI-HBC DONOR|MULTICENTER|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63795,"idioma_id":4,"titulo":"Gas phase imidazole methylation on zeolites and HPA\/SiO2: Study of acid site requirements and catalyst deactivation","resumen":"The gas-phase alkylation of imidazole with methanol was studied at 523 K on HPA\/SiO2 and zeolites HMCM22, HBEA, NaY, ZnY and&nbsp; HY. The nature, density and strength of acid sites were determined by temperature programmed desorption of NH3 coupled with infrared spectra of adsorbed pyridine. Coke formation was studied by temperature programmed oxidation technique. On all the samples, the&nbsp; selectivity to N-methylimidazole was greater than 98%.&nbsp; Catalysts presenting essentially Lewis (NaY and ZnY) or Brønsted (HPA\/SiO2) acidity did not promote efficiently the methylation of imidazole and yielded less than 20% of N-methylimidazole. &nbsp;In contrast, on samples containing similar concentration of Lewis and Brønsted acid sites (HY, HBEA, HMCM22) the N-methylimidazole yield was between 60 % (HMCM22) and 100% (HY). &nbsp;All the samples deactivated during the 4 h catalytic tests and formed significant amounts of coke, between 2.3% (NaY) and 8% (ZnY). Catalytic tests performed at different contact times showed that the initial activity decay diminished with increasing imidazole conversion, suggesting that catalyst deactivation is mainly related with the presence of the reactants, methanol and\/or imidazole. Additional catalytic tests using different feed compositions showed that&nbsp; the initial catalyst deactivation followed a linear correlation with the partial pressure of imidazole which indicated that the activity decay is essentially related with the strong adsorption of imidazole on surface acid sites. ","palabras_clave":"Imidazole methylation|N-methylimidazole|catalyst deactivation|solid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63796,"idioma_id":4,"titulo":"Evaluation of performance of an accelerator-based BNCT facility for the treatment of different tumor targets","resumen":"Purpose: Encouraging Boron Neutron Capture Therapy (BNCT) clinical results obtained in recent years have stimulated intense research to develop accelerator-based neutron sources to be installed in clinical facilities. In this work an assessment of an accelerator-based {BNCT} facility for the treatment of different tumor targets was performed, comparing the accelerator-derived results with reported reactor-based trials under similar conditions and subjected to the same clinical protocols. Materials and methods A set of real image studies was used to cover clinical-like cases of brain and head-and-neck tumors. In addition, two clinical cases of malignant nodular melanoma treated at the RA-6 {BNCT} facility in Argentina were used to thoroughly compare the clinical dosimetry with the accelerator-derived results. Results The minimum weighted dose delivered to the clinical target volume was higher than 30 Gy and 14 Gy for the brain tumor and head-and-neck cases, respectively, in agreement with those achieved in clinical applications. For the melanoma cases, the minimum tumor doses were equal or higher than those achieved with the RA-6 reactor for identical field orientation and protocol. The whole-body dose assessment showed that the maximum photon-equivalent doses for those normal organs close to the beam direction were below the upper limits considered in the protocols used in the present work. Conclusions The obtained results indicate not only the good performance of the proposed&nbsp;beam shaping assembly design associated to the facility but also the potential applicability of accelerator-based BNCT in the treatment of both superficial and deep-seated tumors.","palabras_clave":"Accelerator-based neutron source|Treatment planning assessment|Whole-body dose assessment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63797,"idioma_id":4,"titulo":"Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents","resumen":"Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents.  The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.","palabras_clave":"microbial source tracking|pollution microbiology|fecal pollution|Bacteroidetes|quantitative real-time PCR|genetic markers|water quality"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63798,"idioma_id":4,"titulo":"Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells","resumen":"Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS.","palabras_clave":"DENDRITIC|SUPERANTIGENS|TRAFFICKING|IMMUNITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63799,"idioma_id":4,"titulo":"Characterisation of inclusions in resulphurised microalloyed steel","resumen":"Resulphurised steels, due to their good mechanical properties and machinability, are employed inthe manufacture parts of trucks and high strength components. The microstructure control ofthese steels can improve their toughness without losing strength. In this sense, the chemicalcomposition, morphology and distribution of MnS achieved with different thermomechanical processes affect the mechanical properties of the steel. In this work, the morphology and distribution of MnS inclusions in resulphurised steel 15V45 were analysed. Samples of the steel in its as cast condition and after hot rolling were examined using light and scanning electron (SEM)microscopies. Microhardness measurements and chemical composition of different MnS types were carried out to evaluate the plastic behaviour of each inclusion. The SEM images have provided information about the nucleation sites and size of the inclusions. Applying a Fe-Mn-S diagram under different thermomechanical conditions, this study could predict ductility problems and crack generation during hot rolling processes.","palabras_clave":"microalloyed steel|resulphurized|MnS|Inclusions|Microscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63800,"idioma_id":5,"titulo":"Renuncias presidenciales, opinión pública y legitimidad en el quinquenio difícil (1890-1895)","resumen":"La Revolución del Noventa impulsó importantes cambios en el escenario político argentino de finales del siglo XIX. Ente 1890 y 1895 un proceso de ampliación y diversificación política trastocó las bases del orden conservador que el Partido Autonomista Nacional (PAN) había instaurado en 1880. El propósito de este trabajo es sugerir algunas líneas para analizar la crisis política que se desenvolvió durante esos años atendiendo, en particular, al papel que cumplió la opinión pública como eje articulador de críticas y cuestionamientos al régimen de hegemonía del PAN. Con esa finalidad, hemos elegido situarnos en dos coyunturas especiales como fueron las renuncias de los presidentes Miguel Juárez Celman (en agosto de 1890) y Luis Sáenz Peña (en enero de 1895). Ambos momentos marcan el inicio y el cierre de un ciclo, e instalan en un primer plano el problema de la construcción y la reproducción de la legitimidad política.","palabras_clave":"RENUNCIA PRESIDENCIAL|OPINIÓN PÚBLICA|LEGITIMIDAD|SIGLO XIX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63801,"idioma_id":4,"titulo":"On asteroidal sets in chordal graphs","resumen":"We analyze the  relation between three parameters of a chordal  graph $G$: the number  of non-separating cliques $nsc(G)$, the  asteroidal number $an(G)$ and the leafage $l(G)$. We  show that  $an(G)$ is equal to the maximum value of $nsc(H)$ over all  connected induced subgraphs $H$ of $G$.  As a corollary, we prove  that if $G$ has no   separating  simplicial cliques then  $an(G)=l(G)$.    A graph $G$ is minimal $k$-asteroidal if $an(G)=k$ and $an(H)3$; for $k=3$ it is the family described by Lekerkerker and  Boland to characterize interval graphs. We prove that, for every  minimal $k$-asteroidal chordal graph, all the  above parameters  are equal to $k$. In addition, we characterize the split graphs  that are minimal $k$-asteroidal and obtain all the minimal  $4$-asteroidal split graphs. Finally, we applied our results on  asteroidal sets to describe the clutters with $k$ edges that are  minor-minimal in  the sense that every minor has less than $k$  edges.","palabras_clave":"chordal graphs|asteroidal sets|Sperner families"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63802,"idioma_id":5,"titulo":"Estimación de tasas de predación de Mnemiopsis leidyi (Ctenophora: Lobata) bajo condiciones de laboratorio","resumen":"Se presentan los primeros resultados en el desarrollo de una metodología estandarizada para estimar las tasas de ingestión de Mnemiopsis leidyi y su impacto sobre el reclutamiento de peces. Se evaluó si M. leidyi era capaz de consumir huevos liofilizados de peces para luego estimar las tasas de ingestión sobre este tipo de presas, nauplii de Artemia y rotíferos. Aunque M. leidyi es capaz consumir huevos de peces liofilizados, hemos observado que el consumo de huevos fue muy bajo e incluso frecuentemente nulo, y que en todos los casos fue menor que el estimado para los otros tipos de presa ofrecidos. Por otro lado, la tasa de alimentación sobre cada una de las presas aumentó con la talla de los ctenóforos. Los resultados observados sugieren que esta especie no sería capaz de afectar el reclutamiento de peces a través del consumo de sus huevos. No obstante, no existe en la literatura una metodología estandarizada que permita analizar el problema en forma comparativa. Los resultados obtenidos en este trabajo resaltan la necesidad de definir una metodología estándar que nos permita abordar el estudio de la alimentación de M. leidyi y sus efectos sobre el reclutamiento de peces. El desarrollo de una presa modelo de experimentación representará un paso adelante en este sentido. Para ello será necesario repetir los experimentos presentados en este trabajo utilizando huevos vivos en comparación con los liofilizados.","palabras_clave":"PLANCTON GELATINOSO|CTENOFOROS|RECLUTAMIENTO|MAR ARGENTINO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63803,"idioma_id":4,"titulo":"Effect of the pore geometry in the characterization of the pore size distribution of activated carbons","resumen":"n this work, the characterization of Activated Carbons (AC) by using the independent pore models is discussed, with special emphasis on the issue of how the assumed pore geometry can affect the resulting Pore Size Distribution (rPSD) and on the problem of the unicity of the PSD when different probe molecules are used in adsorption experiments. A theoretical test was performed using virtual solids based in the so-called Mixed Geometry Model (MGM) (Azevedo et al. 2010). The MGM uses a kernel of adsorption isotherms generated by GCMC for different pore sizes and two pore geometries: slit and triangular. The adsorption isotherms of a virtual MGM solid were fitted with both the traditional Slit Geometry Model (SGM) and the Mixed Geometry Model (MGM). It is demonstrated that, by assuming a different pore geometry model from that of the real sample, different PSDs may be obtained by fitting adsorption isotherms of different probe gases. Finally, experimental results are shown which both point toward the MGM as an acceptable extension of the SGM and confirm that the MGM is a closer representation of the actual porous structure of most activated carbons.","palabras_clave":"Activated Carbons|PSD|Unicity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63804,"idioma_id":4,"titulo":"Mate Marote: a flexible automated framework for large-scale educational interventions","resumen":"There is big consensus that computer games may be an  effective way of learning and many initiatives are being developed where  aspects from cognitive sciences are being applied in the development of these games.  In this article, we present Mate Marote, a flexible framework for large-scale  educational interventions. Based on the delivery programs of computers to each  student in Argentinian schools, we developed an environment that provides  activities\/games and registers usage statistics. This framework keeps installation  up-to-date connecting with a central server as Internet connection is detected,  synchronizing new activities, version updates and usage history. As a first  testbed intervention, we deployed three games in La Rioja province (Argentina),  where OLPC is the official program. These games were focused on training  inhibitory control, working memory and planning skills. We found that usage statistics  of games replicate previous results found at the laboratory, showing that this  platform works as an intervention framework despite its unsupervised nature.","palabras_clave":"educational neuroscience|executive functions|cognitive training|computer games"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63805,"idioma_id":4,"titulo":"The VMP1-Beclin 1 interaction regulates autophagy induction","resumen":"The VMP1-Beclin 1 interaction regulates autophagy induction Maria I. Molejon, Alejandro Ropolo, Andrea Lo Re, Veronica Boggio &amp; Maria I. VaccaroScientific Reports 3, Article number: 1055 doi:10.1038\/srep01055The Vacuole Membrane Protein 1 -VMP1- is a pancreatitis-associated transmembrane protein whose expression triggers autophagy in several human diseases. In the current study, we unveil the mechanism through which this protein induces autophagosome formation in mammalian cells. We show that VMP1 autophagy-related function requires its 20-aminoacid C-terminus hydrophilic domain (VMP1-AtgD). This is achieved through its direct binding to the BH3 motif of Beclin 1 leading to the formation of a complex with the Class III phosphatidylinositol-3 kinase (PI3K) hVps34, a key positive regulator of autophagy, at the site where autophagosomes are generated. This interaction also concomitantly promotes the dissociation of Bcl-2, an autophagy inhibitor, from Beclin 1. Moreover, we show that the VMP1-Beclin 1-hVps34 complex favors the association of Atg16L1 and LC3 with the autophagosomal membranes. Collectively, these findings reveal that VMP1 expression recruits and activates the Class III PI3K complex at the site of autophagosome formation during mammalian autophagy.","palabras_clave":"VMP1|Beclin 1|AUTOPHAGY|AUTOPHAGOSOME FORMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63806,"idioma_id":4,"titulo":"EFFECTS OF TRANS FATTY ACIDS ON LIVER LIPID METABOLISM IN MICE FED ON DIETS SHOWING DIFFERENT FATTY ACID COMPOSITION","resumen":"Aim: Our aim was to investigate the effects of trans fatty acids (TFA) on liver lipid metabolism in mice fed on experimental diets rich in either oleic or linoleic acid. Methods: 22 male CF1 mice (22.0±0.1g) were fed with diets rich in corn oil or olive oil, supplemented or not with TFA (0.75g TFA\/100g diet), for 4 weeks. Changes in triacylglycerol content, the activity and expression of enzymes involved in lipogenesis and fatty acid oxidation were measured. &nbsp; Results: Supplementation of an olive oil-rich diet with TFA increased liver triacylglycerols, the activity and expression of lipogenic enzymes and SREBP1a expression. By contrast, when TFA were added to a corn oil-rich diet they did not modify these parameters. No significant differences were observed among experimental groups in the activity and expression of CPT-Ia, body and liver weights or serum triacylglycerol concentrations. Conclusions: The effect of TFA on liver fat accumulation depends on the dietary fatty acid composition. Steatosis induced by TFA when included in olive oil diet, but not in corn oil diet, was associated with an increased lipogenesis but not to a decreased fatty acid oxidation in animals fed on the olive oil diet. This metabolic change is mediated by SREBP1a, but not by SREBP1c, and seems to be independent of insulin. ","palabras_clave":"Trans Fatty acids|fatty acid oxidation|lipogenesis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63807,"idioma_id":4,"titulo":"VMP1 IS A NEW PLAYER IN THE REGULATION OF THE AUTOPHAGY-SPECIFIC PHOSPHATIDYLINOSITOL 3-KINASE COMPLEX ACTIVATION","resumen":"We have elucidated a novel mechanism through which the autophagy-specific class III phosphatidylinositol 3-kinase (PtdIns3K) complex can be recruited to the PAS in mammalian cells, through the interaction between BECN1 and the vacuole membrane protein 1 (VMP1), an integral autophagosomal membrane protein. This interaction involves the binding between the C-terminal 20 amino acids of the VMP1 hydrophilic domain, which we have named the VMP1 autophagy-related domain (VMP1-AtgD), and the BH3 domain of BECN1. The association between these two proteins allows the formation of the autophagy-specific PtdIns3K complex, which activity favors the generation of phosphatidylinositol-3-phosphate (PtdIns3P) and the subsequent association of the autophagy-related (ATG) proteins, including ATG16L1, with the phagophore membranes. Therefore, VMP1 regulates the PtdIns3K activity on the phagophore membrane through its interaction with BECN1. Our data provide a novel model describing one of the key steps in phagophore assembly site (PAS) formation and autophagy regulation, and positions VMP1 as a new interactor of the autophagy-specific PtdIns3K complex in mammalian cells.","palabras_clave":"VMP1|PTDINS3K COMPLEX|BECLIN 1|PHAGOPHORE ASSEMBLY SITE FORMATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63808,"idioma_id":5,"titulo":"NUEVOS REGISTROS DE GARRAPATAS (ACARI: IXODIDAE) ASOCIADAS A ROEDORES SIGMODONTINOS EN CHUBUT, ARGENTINA","resumen":"Se reportan nuevos hallazgos de garrapatas obtenidas de roedores capturados en sitios cercanos a Cholila (42o31\u2019S, 71o27\u2019W), provincia del Chubut (Argentina). Se registraron las siguientes asociaciones parásito-hospedador: Oligoryzomys  lonngicaudatus: 1 larva de Amblyomma tigrinum, 1 hembra de Ixodes sigelos, 2 ninfas de I. sigelos; Reithrodon auritus: 1 hembra de I. sigelos; Loxodontomys micropus: 1 ninfa de I. sigelos; Chelemys macronyx: 7 larvas de I. sigelos. Ixodes sigelos no ha sido reportada previamente en C. macronyx, así como tampoco A. tigrinum sobre O. longicaudatus. Estos resultados brindan nueva evidencia del rol fundamental que cumplen los roedores en el ciclo biológico de algunas garrapatas presentes en la Argentina.","palabras_clave":"Ixodes|Amblyomma|sigmodontinos|Patagonia|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63809,"idioma_id":4,"titulo":"Reciprocal influences between leptin and glucocorticoids during acute Trypanosoma cruzi infection","resumen":"Leptin and glucocorticoids (GCs) are involved in metabolic functions, thymic homeostasis and immune activity through complex interactions. We recently showed that C57BL\/6 mice infected with Trypanosoma cruzi revealed a fatal disease associated to a dysregulated immune-endocrine response characterized by weight loss, deleterious synthesis of pro-inflammatory cytokines and GCs-driven thymus atrophy. Extending this study, we now explored the relationship between leptin and GCs, in terms of infection outcome, thymic and metabolic changes. T. cruzi-infected mice showed a food intake reduction, together with hypoglycemia and lipolysis-related changes. Infected animals also displayed a reduction in systemic and adipose tissue levels of leptin, paralleled by a down-regulation of their receptor (ObR) in the hypothalamus. Studies in infected mice subjected to adrenalectomy (Adx) showed a worsened course of infection accompanied by even more diminished systemic and intrathymic leptin levels, for which GCs are necessary not only to decrease inflammation but also to sustain leptin secretion. Adx also protected from thymic atrophy, independently of the reduced leptin contents. Leptin administration to infected mice aggravated inflammation, lowered parasite burden and attenuated GCs release, but did not normalize thymic atrophy or metabolic parameters.   Acute T. cruzi infection in C57BL\/6 mice, coexist with a dysregulation of leptin\/hypothalamic ObR circuitry dissociated from body weight and food intake control. Endogenous GCs production attempted to reestablish systemic leptin concentrations, but failed to improve leptin-protective activities at the thymic level, suggesting that the leptin\/GCs intrathymic relationship is also altered during this infection.","palabras_clave":"Glucocorticoids|Leptin|Chagas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63810,"idioma_id":4,"titulo":"PRODAN Dual Emission Feature to Monitor BHDC Interfacial Properties Changes with the External Organic Solvent Composition","resumen":"We have investigated the water\/benzyl-n-hexadecyldimethylammonium chloride (BHDC)\/n-heptane:benzene reverse micelles (RMs) interfaces properties using 6-propionyl-2-(N,N-dimethyl)aminonaphthalene,   PRODAN, as molecular probe. We have used absorption and emission   (steady-state and time-resolved) spectroscopy of PRODAN to monitor the   changes in the RMs interface functionalities upon changing the external   organic solvent blend. We demonstrate that PRODAN is a useful probe to   investigate how the external solvent composition affects the micelle   interface properties. Our results show that changes in the organic   solvent composition in water\/BHDC\/n-heptane:benzene RMs have a   dramatic effect on the photophysics of PRODAN. Thus, increasing the   aliphatic solvent content over the aromatic one produces PRODAN   partition and PRODAN intramolecular electron transfer (ICT) processes.   Additionally, the water presence in these RMs makes the PRODAN ICT   process favored with the consequent decreases in the LE emission   intensity and a better definition of the charge transfer (CT) band. All   this evidence suggests that the benzene molecules are expelled out of   the interface, and the water\u2013BHDC interactions are stronger with more   presence of water molecules in the polar part of the interface. Thus, we   demonstrate that a simple change in the composition of the external   phase promotes remarkable changes in the RMs interface. Finally, the   results obtained with PRODAN together with those reported in a previous   work in our lab reveal that the external phase is important when trying   to control the properties of RMs interface. It should be noted that the   external phase itself, besides the surfactant and the polar solvent   sequestrated, is a very important control variable that can play a key   role if we consider smart application of these RMs systems.","palabras_clave":"COLOIDES|SOLVATACION|NANOESTRUCTURAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63811,"idioma_id":4,"titulo":"Kinetics of colour and texture changes of beef during storage","resumen":"The influence of the packaging films, aerobic and vacuum conditions, and refrigeration storage temperature (0, 4 and 10 °C) on colour and texture of raw meat were studied during a 2-week storage period in order to analyze kinetics of colour and texture changes. The rate of redness decrease was  most noticeable at the highest storage temperature and aerobic conditions and these changes were well described by the firstorder reaction. Texture parameter reflected a progressive softening during storage for both films. Decrease on shear force of raw beef during storage followed a first-order kinetic model. Temperature dependence of colour and texture change was  adequately modelled with the Arrhenius equation.","palabras_clave":"Shelf life|Maximum shear force| Storage temperature|Packaging films"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63812,"idioma_id":4,"titulo":"OXA-258 from Achromobacter ruhlandii: a Species Specific Marker","resumen":"A new blaOXA-258 gene is described as a species-specific taxonomic marker for Achromobacter ruhlandii isolates (all recovered from cystic fibrosis patients). Even though OXA-258 differs from OXA-114 variants, isolates could be misidentified as A. xiloxosidans by the amplification of an inner fragment from the OXA-coding gene. A robust identification of A. ruhlandii can be achieved by sequencing this single OXA gene, as well as by a more laborious recently proposed multilocus sequence-typing (MLST) scheme.","palabras_clave":"OXA-258|Achromobacter ruhlandii"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63813,"idioma_id":5,"titulo":"Evaluación de las creencias sobre extensión rural de los extensionistas paraguayos","resumen":"En gran parte de los países latinoamericanos se llevan adelante iniciativas y proyectos de desarrollo y extensión rural orientados a pequeños productores, los cuales incluyen capacitación, provisión de créditos o subsidios y trabajo asociativo. Diversos teóricos han argumentado la existencia de dos modelos diferenciados de extensión, uno transferencista, centrado en la transferencia de tecnologías, y otro dialógico, orientado a la construcción de saberes superadores entre técnicos y productores.       En el presente trabajo se evalúan las creencias fundamentales asociadas a uno y otro modelo en extensionistas paraguayos y el perfil de los técnicos y técnicas que se vinculan con cada uno de ellos. Se concluye que estos modelos, si bien teóricamente contrapuestos, se superponen. Además, se observa una menor presencia de supuestos transferencistas en técnicos de mayor edad y experiencia y en técnicas mujeres.                                                     ","palabras_clave":"Desarrollo |Extensión Rural|Modelo Dialógico|Modelo Transferencista"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63814,"idioma_id":5,"titulo":"La construcción de un currículo intercultural a partir del diálogo de saberes: descripción y análisis de una  experiencia de formación docente continua","resumen":"En este trabajo pretendemos dar a conocer los fundamentos  y algunos resultados de la sistematización y análisis de un módulo curricular  focalizado en el análisis y sistematización de la ciencia formal y factual, que  formó parte del posgrado en Interculturalidad y Bilingüismo, desarrollado en  un centro de formación docente de la provincia de Jujuy (Argentina).  Nuestra intención ha sido revisar la experiencia didáctica, para construir  nuevos aprendizajes y socializarlos con quienes transitan caminos similares.  Hemos identificado algunos aspectos que posibilitarían y fortalecerían el  diseño e implementación de propuestas didácticas interculturales para la  enseñanza de las ciencias naturales, así como aquéllos que funcionarían  como obstaculizadores. Este proceso de formación docente continua posee  la singularidad de haber sido el primero en nuestro país en un posgrado de  actualización docente y, en este sentido, constituye un aporte como  innovación didáctica.","palabras_clave":"Interculturalidad|Curriculum|Formación docente|Enseñanza de las ciencias"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63815,"idioma_id":4,"titulo":"Distribution of Allelic Variants of the Chromosomal Gene bla OXA-114-like in Achromobacter xylosoxidans Clinical Isolates","resumen":"Achromobacter xylosoxidans is increasinglybeing documented in cystic fibrosis patients. The blaOXA-114 gene has been recognized as a naturally occurringchromosomal gene, exhibiting different allelic variants. Inthe population under study, the blaOXA-114-like gene wasfound in 19\/19 non-epidemiological-related clinical isolatesof A. xylosoxidans with ten different alleles including1 novel OXA-114 variant.","palabras_clave":"Achromobacter|OXA-114 variants"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63816,"idioma_id":5,"titulo":"Evaluación de un proceso de capacitación para extensionistas rurales implementado en Paraguay","resumen":"            Se implementó una metodología,  innovadora en el contexto paraguayo, para capacitar extensionistas rurales del  departamento de Caazapá. Esta capacitación se caracterizó por orientarse al  fortalecimiento y a la transformación de las prácticas de extensión, buscando  desarrollar un modelo más horizontal y participativo de interacción. Para esto  se implementaron ocho módulos de capacitación de carácter mensual donde se  incorporaron procesos reflexivos sobre los problemas a los que enfrentan los  extensionistas en su práctica y espacios para la construcción de propuestas  para superarlos. En este artículo se describe y evalúa tanto la implementación  como el impacto de la capacitación en las creencias de los extensionistas,  tanto a nivel cualitativo como cuantitativo. La evidencia muestra que la  metodología implementada transformó significativamente el modo en que los  técnicos comprenden su rol en el trabajo de extensión. En concreto, ahora se  conciben como facilitadores que trabajan de manera conjunta con los productores  en lugar de como quienes tienen que aportar todas las soluciones  ","palabras_clave":"EXTENSIÓN RURAL|CAPACITACIÓN DE EXTENSIONISTAS|PARAGUAY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63817,"idioma_id":4,"titulo":"GREEN METHOD BASED ON A FLOW-BATCH ANALYZER SYSTEM FOR THE SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN PHARMACEUTICALS USING A CHEMOMETRIC APPROACH","resumen":"Green method based on a flow-batch analyzer system for the simultaneous determination of ciprofloxacin and dexamethasone in pharmaceuticals using a chemometric approach","palabras_clave":"-"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63818,"idioma_id":4,"titulo":"The Effect of Age and Sex on Object Exploration and Manipulative Behavior in a Neotropical Raptor, the Chimango Caracara, Milvago chimango","resumen":"We studied object exploration and manipulative behavior in wild-caught Chimango Caracaras (Milvago chimango), an opportunistic and generalist raptor species, in relation to age and sex differences. Each bird was presented with six objects. We then recorded the latencies to approach and first contact with the objects, the number of objects explored, and the number of exploration events performed on each object. Age influenced the tendency to explore in M. chimango. Compared with adults, juveniles were more likely to explore the objects, approaching and contacting them more quickly. The number of objects explored was also higher in young than adult birds. Both age classes used a variety of manipulative behaviors to explore the objects, some of which have been described as play in others studies. Sex did not affect an individual?s likelihood to explore or the number and frequency of manipulative behaviors used during object exploration. The tendency for both young and adult birds to explore and manipulate objects that not resemble prey is likely to be a distinct advantage for a generalist species like M. chimango which must cope with a high diversity of modified environments.","palabras_clave":"OBJECT EXPLORATION|MANIPULATIVE BEHAVIOR|MILVAGO CHIMANGO|BEHAVIOR FLEXIBILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63819,"idioma_id":4,"titulo":"Improvement in the adsorption of thiabendazole by using aluminum pillared clays","resumen":"Many chemical products like fungicides are used in agricultural activities with the purpose of improving the quality and quantity of food production but, contaminating the natural resources with serious consequences to human health and environment.    In this work, the removal of thiabendazole (widely used fungicide in the Alto Valle de Rio Negro-Neuquén, Argentina) by the use of aluminum pillared clays as adsorbents, in comparison with their starting material (a natural clay mineral) was studied. The aim of this work is to improve both thiabendazole remotion by an adsorption process and the posterior extraction of the used adsorbent from the solution, using modified clays instead of the natural clay mineral. The pillared clays were synthesized from a natural clay mineral from the northern Patagonia in Argentina, by using two aluminum oligocations synthesized at different temperatures. The samples were characterized by several techniques, remarking that some structural and textural changes were produced during the pillaring process. The pillared samples showed a considerable increase in their adsorption capacity in comparison to the natural clay mineral. This effect can be related to the textural and structural changes produced during the pillaring process. Additionally, the increase in the hydrophobicity of the pillared samples would allow an easier separation from the aqueous systems. Consequently, aluminum pillared clays could be the new materials to be used in studied environmental process.","palabras_clave":"Thiabendazole removal;  |; Adsorption in solution;|Aluminum pillared clays|Adsorbents"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63820,"idioma_id":4,"titulo":"COMPARATIVE STUDY OF ZEA KARYOTYPES","resumen":"The taxa of genus Zea, maize and teosintes, have both intra and interspecificvariation in its genome size, due to differences in heterochromatin content (Tito et al.Theor. App. Genet. 83:58-64, 1991; Poggio et al. Ann. J. Bot. 82:115-117, 1998). Theheterochromatin mainly forms telomeric or subtelomeric blocks called knobs (Kato,Mass. Agric. Exp. Stn. Bull. 635: 1-185, 1976).A comparative analysis of the karyotypes of different taxa of genus Zea ispresented. In addition, we studied the karyotype variation in the percent and location ofheterochromatic regions, and discuss the inter and intrachromosomal asymmetry foreach karyotype.","palabras_clave":"Zea|karyotypes|asymmetry indexes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63821,"idioma_id":4,"titulo":"Development of Conformation Independent Computational Models for the Early Recognition of Breast Cancer Resistance Protein substrates","resumen":"ATP-Binding Cassette (ABC) efflux transporters are polyspecific members of the ABC superfamily that, acting as drug and metabolite carriers, provide a biochemical barrier against drug penetration and contribute to detoxification. Moreover, their overexpression is linked to multidrug resistance issues in a diversity of diseases (e.g. cancer). Breast Cancer Resistance Protein (BCRP) is the most expressed ABC efflux transporter throughout the intestine and the blood-brain barrier, limiting the oral absorption and brain bioavailability of its substrates. Early recognition of BCRP substrates is thus essential to optimize oral drug absorption, design of novel therapeutics for central nervous system conditions and overcome BCRP-mediated cross-resistance issues. Here, we present the development of an ensemble of ligand-based machine learning algorithms for the early recognition of BCRP substrates, from a database of 262 substrates and non-substrates compiled from literature. Such dataset was rationally partitioned into training and test sets by application of a 2-step clustering procedure. The models have been developed through application of Linear Discriminant Analysis to random subsamples of Dragon molecular descriptors. Simple data fusion and statistical comparison of partial areas under the curve of Receiving Operating Characteristic curves were applied to obtain the best 2-model combination, which presented 82% of overall accuracy in the training set and 74.5% of overall accuracy in the test set. These are remarkable results considering the broad substrate specificity of BCRP. Moreover, Receiving Operating Characteristic curves may be applied to attain an optimal, context-dependent balance between specificity and sensitivity of the model ensemble.","palabras_clave":"BREAST CANCER RESISTANCE PROTEIN|ABC TRANSPORTER|MULTIDRUG RESISTANCE|BCRP SUBSTRATES|2D COMPUTATIONAL MODELS|IN SILICO CLASSIFICATION MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63822,"idioma_id":4,"titulo":"Application of computer-aided drug repurposing in the search of new cruzipain inhibitors: discovery of amiodarone and bromocriptine inhibitory effects","resumen":"Cruzipain (Cz) is the major cystein protease of the protozoan Trypanosoma cruzi, etiological agent of Chagas disease. From a 163-compound dataset, a 2D-classifier capable of identifying Cz inhibitors was obtained and applied in a virtual screening campaign on the DrugBank database, which compiles FDA-approved and investigational drugs. 54 approved drugs were selected as candidates, 4 of which were acquired and tested on Cz and T. cruzi epimastigotes. Among them, the antiparkinsonian and antidiabetic drug bromocriptine and the antiarrhythmic amiodarone showed dose-dependent inhibition of Cz and antiproliferative activity on the parasite.","palabras_clave":"Virtual Screening|Chagas disease|Drug repurposing |Trypanosoma cruzi|Amiodarone|Bromocriptine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63823,"idioma_id":4,"titulo":"The nursing image and professional identity. The future of a construction","resumen":"El Objetivo del estudio fue Describir la imagen que tienen los enfermeros, médicos y otros profesionales de la salud sobre el personal de enfermería. Metodología. Estudio descriptivo realizado en 2010 en una muestra probabilística de 308 participantes (100 enfermeros, 103 médicos y 105 otros profesionales) de Buenos Aires, Argentina. Se emplearon cuestionarios con preguntas cerradas de opción múltiple. Resultados. Entre los principales resultados se encontró una percepción de las condiciones laborales que implican desgaste, impotencia y conflictos en el grupo de trabajo (con más frecuencia entre enfermeros que en los otros grupos de estudio). Asimismo, se encontraron discrepancias entre lo que los enfermeros consideran como sus ´funciones específicas´ y lo que consideran los otros profesionales. Conclusión. Existe tensión entre el rol prescrito por el modelo médico y el que define enfermería. Es necesario concebir cómo la identidad profesional se construye a partir de estrategias identitarias resultantes, a su vez, de estrategias objetivas y subjetivas","palabras_clave":"ENFERMERIA |MEDICOS|EJERCICIO PROFESIONAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63824,"idioma_id":4,"titulo":"Neosiphonia howei (Ceramiales: Rhodomelaceae)\u2014a common epiphyte of the spreading coral reef alga Lobophora variegata (Dictyotales: Dictyotaceae)","resumen":"Fleshy macroalgae are becoming a dominant benthic component on Caribbean     coral reefs, with increased abundance and frequency across all reef     zones. Over the past two decades, the brown alga Lobophora variegata     has proliferated on shallow and deep reefs in Curaçao, former     Netherlands Antilles. This alga provides a living substrate for a     diverse epiphytic community. Here we report on Neosiphonia howei (Rhodomelaceae: Ceramiales), growing epiphytically on L. variegata over a broad depth gradient (6.5 to 40&nbsp;m). Although N. howei has been reported as a typical epiphyte in shallow water, it was commonly found on L. variegata     blades on the reef slope in all fertile stages. This epiphyte   anchored   deeply into the host tissue, suggesting hemiparasitic   behaviour. The   spread of L. variegata may have increased the depth-range of N. howei and the exact nature of the interaction between N. howei and its host deserves further research considering the importance of fleshy macroalgae on coral reefs.","palabras_clave":"Deep coral reefs|Curacao|Phase shift|Turf algae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63825,"idioma_id":4,"titulo":"Increased physiological performance of Halimeda opuntia in response to experimental nutrient enrichment on a Caribbean coral reef.","resumen":"Calcifying green macroalgae of the genus Halimeda are widely     distributed on coral reefs and may become more abundant under higher     nutrient availability. To determine how nutrient enrichment affects the     physiological performance of Halimeda opuntia (Linnaeus)     J.V.Lamouroux in relation to different water depths, we carried out in     situ nutrient enrichment experiments in Curaçao, Netherlands Antilles. H. opuntia     was collected in 5&nbsp;m and then incubated at 5 and 15&nbsp;m in clear   acrylic   cages with or without addition of N and P. Growth, algal   tissue   composition (internal C, N, and P content, &#948;15N   signatures,   protein content), photosynthetic performance and pigment   content were   measured after 14 days of incubation. Growth rates and   total C increased   with nutrients and were higher in 5&nbsp;m water depth. N   and P content were   higher and &#948;15N signatures were lighter   with nutrients in   both depths. Photosynthetic performance,   concentrations of the main and   accessory photosynthetic pigments, and   photoprotection also increased   with nutrients and showed some response   to depth. These results indicate   that nutrient enrichment supported a   rapid increase in physiological   performance of H. opuntia,   but with differences in depth. In   5&nbsp;m, more C was allocated to growth,   where light levels were sufficient,   while in 15&nbsp;m C was allocated to   photosynthetic pigments. These results   suggest that nutrient   enrichment may influence their abundance and   depth distribution on the   reef.","palabras_clave":"Nitrogen and phosphorus limitation|Growth|Carbon allocation|Photoacclimation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63826,"idioma_id":4,"titulo":"Immune response and serum bactericidal activity against Brucella ovis elicited       using a short immunization schedule with the polymeric antigen BLSOmp31 in rams.","resumen":"Brucella ovis is the etiologic agent of ovine brucellosis. The control measures for this disease are periodical diagnosis by serological tests and\/or bacteriological culture and culling of positive animals. Vaccination with Brucella melitensis Rev 1 is recommended when prevalence is high. This attenuated strain vaccine gives protection against B. ovis but it has important disadvantages associated with the development of antibodies interfering with serodiagnosis, virulence for humans and the prohibition of its use in countries considered free of B. melitensis. Consequently, there is a need for new safe and effective brucellosis vaccines to be developed. We have previously reported that the polymeric subcellular vaccine BLSOmp31 confers protection against experimental challenge with B. ovis when rams are immunized three times. In the present work we evaluated and characterized, along 56 weeks after the first immunization of adult rams, the evolution of the immune response elicited by BLSOmp31 using a short immunization schedule.","palabras_clave":"Brucella ovis|BLSOmp31|vaccine|immunization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63827,"idioma_id":4,"titulo":"Short Communication: Fasting Increases Serum Concentrations of Bilirubin in Patients Receiving Atazanavir: Results from a Pilot Study","resumen":"Unconjugatedhyperbilirubinemia resulting from therapy with atazanavir is physiologicallyrelated to hyperbilirubinemia in Gilbert´s syndrome (GS). In patients with GS,changes in diet have a significant impact on bilirubinemia. Our aim was toinvestigate whether changes in diet affect the level of serum bilirubin inpatients receiving atazanavir. Thirty patients on stable therapy withritonavir-boosted atazanavir without evidence of GS were enrolled. Hemolysisand chronic hepatitis were excluded. After a baseline period of normal intakeof calories, the patients were randomized to follow a 24-h 400-calorie diet(fasting), then a 48-h period of normal calorie intake and, afterward, a 24-hperiod of a high-calorie diet, or the same interventions in inverse order.Serum bilirubin concentrations were measured before and after eachintervention. A high adherence to the recommended diet was observed. The meanunconjugated bilirubin concentration before the high-calorie diet was 2.79±1.53&#8201;mg\/dl and after such intervention it was 2.70±1.40&#8201;mg\/dl. The mean difference between preintervention andpostintervention was &#8722;0.08±0.69&#8201;mg\/dl (p=NS). The meanunconjugated bilirubin concentration before the fasting diet was 2.31±1.23&#8201;mg\/dl and it was 3.84±1.90&#8201;mg\/dl after.The mean difference between prefasting and postfasting was 1.53±1.17&#8201;mg\/dl (p=0.001). According to these results, short periods of fastingseem to increase the unconjugated bilirubin concentration in patients onatazanavir. A high-calorie diet did not have any impact in bilirubin probablybecause most patients follow similar diets in their everyday life.","palabras_clave":"AIDS|ATAZANAVIR|HYPERBILIRUBINEMIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63828,"idioma_id":4,"titulo":"Influence of swimming strategy on microorganism separation by asymmetric obstacles","resumen":"Ver","palabras_clave":"SWIMMING STRATEGY|ASYMMETRIC OBSTACLES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63829,"idioma_id":4,"titulo":"Kefir-isolated Lactococcus lactis subsp. lactis inhibits the cytotoxic effect of Clostridium difficile in vitro","resumen":"Kefir is a dairy product obtained by fermentation of milk with a complex microbial population and several health-promoting properties have been attributed to its consumption. In this work, we tested the ability of different kefir-isolated bacterial and yeast strains (Lactobacillus kefir, Lactobacillus plantarum, Lactococcus lactis subps. lactis, Saccharomyces cerevisiae and Kluyveromyces marxianus) or a mixture of them (MM) to antagonize the cytopathic effect of toxins from Clostridium difficile (TcdA and TcdB). Cell detachment assays and F-actin network staining using Vero cell line were performed. Although incubation with microbial cells did not reduce the damage induced by C. difficile spent culture supernatant (SCS), Lc. lactis CIDCA 8221 and MM supernatants were able to inhibit the cytotoxicity of SCS to Vero cells. Fraction of Lc. lactis CIDCA 8221 supernatant containing components higher than 10 kDa were responsible for the inhibitory activity and heating of this fraction for 15 min at 100 °C completely abrogated this ability. By dot-blot assay with anti-TcdA or anti-TcdB antibodies, concentration of both toxins seems to be reduced in SCS treated with Lc. lactis CIDCA 8221 supernatant. However, protective effect was not affected by treatment with proteases or proteases-inhibitors tested. In conclusion, we demonstrated that kefir-isolated Lc. lactis CIDCA 8221 secreted heat-sensitive products able to protect eukaryotic cells from cytopathic effect of C. difficile toxins in vitro.  Our findings provide new insights into the probiotic action of microorganisms isolated from kefir against virulence factors from intestinal pathogens.","palabras_clave":"LACTOCOCCUS LACTIS|CLOSTRIDIUM DIFFICILE |IN VITRO|TOXINS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63830,"idioma_id":4,"titulo":"Protective effect of a mixture of kefir-isolated lactic acid bacteria and yeasts in a hamster model of Clostridium difficile infection","resumen":"The objective of this work was to test the protective effect of a mixture (MM) constituted by kefir-isolated microorganisms (Lactobacillus plantarum, Lactobacillus kefir, Lc. lactis, Kluyveromyces marxianus and  Saccharomyces cerevisiae) in a hamster model of infection with Clostridium difficile, an anaerobic Grampositive bacterium that causes diarrhoea. Placebo or MM was administered ad libitum in drinking water  from day 0 to the end of treatment. Hamsters received orally 200 mg of clyndamicin at day 7 and then were infected with 1  108 CFU of C. difficile by gavage. Development of diarrhoea and death was registered until the end of the protocol. Surviving animals were sacrificed at day 16, and a test for biological activity of clostridial toxins and histological stainings were performed in caecum samples. Six of seven infected animals developed diarrhoea and 5\/7 died at the end of the experimental protocol. The histological sections showed oedema and inflammatory infiltrates with neutrophils and crypt abscesses.  In the group of animals infected and treated with MM1\/1000, only 1 of 7 hamsters showed diarrhoea and none of them died. The histological sections showed only a slight thickening of the mucosa with presence  of lymphocytic infiltrate. These results demonstrate that an oral treatment with a mixture of kefirisolated bacteria and yeasts was able to prevent diarrhoea and enterocolitis triggered by C. difficile.","palabras_clave":"CLOSTRIDIUM DIFFICILE|KEFIR|HAMSTERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63831,"idioma_id":4,"titulo":"Late Cenozoic Foraminifera from diamictites of Cape Lamb, Vega Island, Antarctic Peninsula","resumen":"Abstract. Foraminiferal assemblages recovered from Cape Lamb sediments on Vega Island are examined and illustrated herein. The foraminifera were discovered in Cenozoic debris and palagonitic breccia lithofacies that crop out at the southwestern tip of Cape Lamb. Amongall the Cenozoic diamictite deposits recognized in this island, the studied sediments are the youngest and the most fossiliferous. Pectinids,brachiopods, and microfaunas are exceptionally well preserved and indicate little or no transport. The foraminiferal assemblage is dominatedby infaunal species, with the most abundant including Globocassidulina biora, Cribroelphidium sp. aff. E. excavatum, and Cassidulinoides parkerianus, and the epifaunal Cibicides refulgens. These foraminifera suggest a normal inner shelf marine environment. Microfossils and macrofauna, stratigraphical evidence observed in the field and published isotopic ages from the nearby Cape Lamb suggest a Pleistocene age for this deposit.","palabras_clave":"FORAMINIFERA|DIAMICTITE|CENOZOIC|ANTARCTICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63832,"idioma_id":4,"titulo":"Qualitative autoradiography with polycarbonate foils enables histological and track analyses on the same section","resumen":"Neutron autoradiography is an imaging methodology that enables analysis of the spatial distributionof heavy ion emitters in a given material. In particular, it allows localization of 10 B in atissue section put in contact with a nuclear track detector. Boron imaging is essential when consideringboron neutron capture therapy as an option for treating cancerous tumors. A descriptionof the autoradiography method is presented together with specifi c characteristics and technicaldetails developed in our laboratory. We propose a new mounting technique to compare autoradiographyimages with the same section that gave rise to the latent tracks. The solid state nucleartrack detector is polycarbonate, because it can be processed rapidly to obtain the autoradiographicresults. It is a transparent material, which allows visualization of the sections mountedon it. Tissue can be removed easily and background is minimal.","palabras_clave":"boron imaging|histochemical localization|neutron autoradiography|SSNTD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63833,"idioma_id":4,"titulo":"The aestivation chamber of the giant earthworm Glossoscolex bergi (Glossoscolecidae) in the subtropical rainforest of Misiones (Argentina)","resumen":"A new ichnotaxon, attributed to earthworm aestivation chambers, is described from paleosols of the Sopas Formation (Upper Pleistocene) of northern Uruguay. This ichnofossil consists of a spherical chamber with a constructed wall of imbricated faecal pellets and a filling of rounded to meniscate pellets arranged in winding strings. The strings may also be connected to specimens of Taenidium serpentinum in the paleosol, resulting in a compound trace fossil. Castrichnus incolumis igen. et isp. nov. is interpreted as an earthworm aestivation chamber based on its morphological similarity to the chambers produced by extant earthworms. As such, C. incolumis is considered an indicator of subaerial exposure, and also of seasonal climate. C. incolumis is the second trace fossil that can be attributed with certainty to earthworms. In addition, the Castrichnus?T. serpentinum compound specimens indicate that in paleosols, earthworms can be the producers of T. serpentinum. Aestivation chambers would represent a new ethological category.","palabras_clave":"EARTHWORM|GLOSSOSCOLEX BERGI |SUBTROPICAL RAINFOREST|CASTRICHNUS INCOLUMIS|PALAEOENVIRONMENTAL INDICATOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63834,"idioma_id":4,"titulo":"An individual-based model to estimate the daily energetic cost of Greater Rheas and its contribution on population recruitment","resumen":"An individual-based model for estimating the energetic costs in Rhea americana was developed considering their sexual and seasonal differences in the behavioral activities. The model includes as variables the individual's characteristics, as well as corporal weight, the time spent on different activities, and the cost associated with each activity. We estimated the daily energetic demand of an adult rhea based on the activities individuals normally develop during postreproductive, nonreproductive, and reproductive seasons, differentiating between sexes. The time spent in each activity for one given animal was calculated from field observations of individuals and the estimations of energetic costs for each activity were obtained from specialized literature. The model built varied between sexes because males and females have different reproductive costs. Both models have the same general formulation but they differ in the cost associated with reproduction. In Greater Rheas, while males assume all of the incubation, the females only lay eggs communally in a single nest. Also the possibility that the individual reproduces or not was considered. The model does not allow to determine whether the energetic costs associated with the breeding are the reason why few individuals try to reproduce but it indicates that there is a clear difference in the daily energetic costs of individuals which reproduce and those which do not reproduce. Other activities associated with parental care posthatching, not taken into account here, would increase these differences, and would explain the low number of breeding attempts observed at wild.","palabras_clave":"ENERGETIC COSTS|INDIVIDUAL BEHAVIOR|RHEA AMERICANA|REPRODUCTIVE COSTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63835,"idioma_id":4,"titulo":"The NSAIDs Indomethacin and Diflunisal as scavengers of  photogenerated reactive oxygen species","resumen":"Diflunisal (DFNS) and Indomethacin (IMTC) are two profusely  employed NSAIDs that provide anti-inflammatory and analgesic effects in humans.  The scavenging of reactive oxygen species (ROS) by both NSAIDs was  systematically studied in pH 7 aqueous solution. The ROS O2(1Dg), O2·? and  H2O2, generated  by visible light irradiation of Riboflavin  in the presence of DFNS and IMTC, are deactivated by the NSAIDs.               The ROS  scavenging action by both NSAIDs  constitutes an interesting result and adds  one more positive aspect to the beneficial actions attributed to these drugs. Nevertheless  it should be taken into account that several NSAIDs, in particular IMTC, have  been connected to the pathogenesis of gastric mucosal lesions, which in some cases includes ROS  generating-ability.              DFNS quenches  ROS in a dominant physical fashion. It constitutes an excellent protective-antioxidant  provided that is practically not destroyed\/oxidized after the ROS scavenging  action. IMTC, being also an efficient interceptor of ROS, belong to the  so-called group of sacrificial-ROS quenchers:  It is easily degraded by the oxidative  species in the scavenging action. Although this property is negative in the  context of prolonged ROS elimination, exhibits a promissory aspect for the  degradation of pharmaceutical contaminants, such as NSAIDs, in waste waters. ","palabras_clave":"DIFLUNISAL|INDOMETHACIN|NSAIDS|QUENCHING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63836,"idioma_id":4,"titulo":"Parent-progeny relationships between maize inbreds and hybrids: Analysis of grain yield and its determinants for contrasting soil nitrogen conditions","resumen":"Most  research in maize (Zea mays L.) parent¨Cprogeny  relationships has focused on heterosis for plant grain yield (PGY)  determination, whereas nonheterotic effects for traits other than PGY has  remained less explored. Our objectives were to analyze (i) frequency  distribution and phenotypic plasticity for 29 eco-physiological traits in  different genotypic groups (6 inbreds and 12 hybrids) and environments, (ii)  parent¨C progeny relationships for these traits as well as variations in these  relationships caused by contrasting growing conditions, and (iii) direct and indirect  effects of traits measured in inbreds on hybrid PGY determination. Genotypes  were cropped in the field at two contrasting N levels during three growing  seasons. Range in phenotypic plasticity was (i) similar for inbreds and hybrids,  (ii) largest for traits such as PGY and nitrogen use efficiency (NUE), and (iii)  smallest for traits such as time to flowering and kernel weight. Inbred  phenotype was usually (26 traits) a good predictor of hybrid phenotype, but  analysis of standardized data demonstrated that (i) for nine traits (e.g., PGY,  kernel numbers) this relationship was exclusively driven by environmental effects,  and (ii) for the other traits there was a true genetic control. A high  correlation (r &gt; 0.26; P ¡Ü  0.024)  was established betweenhybrids PGY and 12 traits measured in inbreds, among  which we distinguished NUE and eargrowth rate for their high direct effect and participation  in the indirect effect of other traits.","palabras_clave":"MAIZE|PARENT-PROGENY RELATIONSHIPS|GRAIN YIELD|NITROGEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63837,"idioma_id":4,"titulo":"Macrophytes, epipelic biofilm, and invertebrates as biotic indicators of physical habitat degradation of lowland streams (Argentina)","resumen":"Our objective was to assess the effect of the physical habitat degradation in three lowland streams of Argentina that are subject to different land uses. To address this matter, we looked into some physical habitat alterations, mainly the water quality and channel changes, the impact on macrophytes? community, and the structural and functional descriptors of the epipelic biofilm and invertebrate assemblages. As a consequence of physical and chemical perturbations, we differentiated  sampling sites with different degradation levels. The low degraded sites were affected mainly for the suburban land use, the moderately degraded sites for the rural land use, and the highly degraded sites for the urban  land use. The data shows that the biotic descriptors that best reflected the environmental degradation were vegetation cover and macrophytes richness, the dominance of tolerant species (epipelic biofilm and invertebrates), algal biomass, O2 consumption by the epipelic biofilm, and invertebrates? richness and diversity. Furthermore, the results obtained highlight the importance of the macrophytes in the lowland streams, where there is a poor diversification of abiotic substrates and where the macrophytes not only provide shelter but also a food source for invertebrates and other trophic levels such as fish. We also noted that both in benthic communities, invertebrates and epipelic biofilm supplied different information: the habitat?s physical structure provided by  the macrophytes influenced mainly the invertebrate descriptors; meanwhile, the water quality mainly influenced most of the epipelic biofilm descriptors.","palabras_clave":"Macrophytes|Epipelic biofilm|Lowland streams"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63838,"idioma_id":4,"titulo":"Brucella abortus induces TNF-alpha-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases","resumen":"Background: Central nervous system (CNS) invasion by bacteria of the genus Brucella results in an inflammatory disorder called neurobrucellosis. We have recently demonstrated that B. abortus infects microglia and astrocytes, eliciting the production of a variety of pro-inflammatory cytokines which contribute to CNS damage. Matrix metalloproteinases (MMP) have been implicated in inflammatory tissue destruction in a range of pathological situations in the CNS. Increased MMP secretion is induced by pro-inflammatory cytokines in a variety of CNS diseases characterized by tissue-destructive pathology.  Methods: In this study, the molecular mechanisms that regulate MMP secretion from Brucella-infected astrocytes in vitro were investigated. MMP-9 was evaluated in culture supernatants by ELISA, zymography and gelatinolytic activity. Involvement of mitogen-activated protein kinases(MAPK) signaling pathways was evaluated by Western blot and using specific inhibitors. The role of TNF-&#945; was evaluated by ELISA and by assays with neutralizing antibodies. Results: B. abortus infection induced the secretion of MMP-9 from murine astrocytes in a dose-dependent fashion. The phenomenon was independent of bacterial viability and was recapitulated by L-Omp19, a B. abortus lipoprotein model, but not its LPS. B. abortus and L-Omp19 readily activated p38 and Erk1\/2 MAPK, thus enlisting these pathways among the kinase pathways that the bacteria may address as they invade astrocytes. Inhibition of p38 or Erk1\/2 significantly diminished MMP-9 secretion, and totally abrogated production of this MMP when both MAPK pathways were inhibited simultaneously. A concomitant abrogation of B. abortus- and L-Omp19-induced TNF-&#945; production was observed when p38 and Erk1\/2 pathways were inhibited, indicating that TNF-&#945; could be implicated in MMP-9 secretion. MMP-9 secretion induced by B. abortus or L-Omp19 was completely abrogated  when experiments were conducted in the presence of a TNF-&#945; neutralizing antibody. MMP-9 activity was detected in cerebrospinal fluid (CSF) samples from patients suffering from neurobrucellosis.  Conclusions: Our results indicate that the inflammatory response elicited by B. abortus in astrocytes would lead to the production of MMP-9 and that MAPK may play a role in this phenomenon. MAPK inhibition may thus be  considered as a strategy to control inflammation and CNS damage in neurobrucellosis.","palabras_clave":"BRUCELLA ABORTUS|NEUROBRUCELLOSIS|ASTROCYTES|MATRIX METALLOPROTEINASES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63839,"idioma_id":4,"titulo":"Gelation, thermal and pasting properties of pigeon pea (Cajanus cajan L.), dolichos bean (Dolichos lablab L.) and jack bean (Canavalia ensiformis) flours","resumen":"This study evaluated the rheological, thermal and pasting properties of pigeon pea (PP), dolichos bean (DB) and jack bean (JB) legume flours and gels. Starch and protein contents were also measured and its molecular weight distribution was determined by electrophoresis. PP and DB showed the highest viscosities while JB had the highest pasting temperature. The minimum flour concentrations for gel formation were estimated at 6?8% for DB and PP and 10% for JB. Above these concentrations all flour suspensions heated to 95 C led to gels with a solid-like behavior. Differential scanning calorimetry showed two endothermic peaks in all flours at 80?89 C and 96?100 C. Avrami model was successfully fitted to the hardening kinetics of PP and DB gels stored at 4 C. The half-life times were 22 and 6 h for PP and DB respectively. PP and DB flours were able to form self-supporting gels and could be applied in the formulation of gel-like foods.","palabras_clave":"GELS|LEGUMES|RHEOLOGICAL PROPERTIES|DIFFERENTIAL SCANNING CALORIMETRY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63840,"idioma_id":4,"titulo":"Chasing the Patagonian sun: comparative thermal biology of Liolaemus lizards","resumen":"The importance of the thermal environment for ectotherms and its relationship with thermal physiology and ecology is widely recognized. Several models have been proposed to explain the evolution of the thermal biology of ectotherms, but experimental studies have provided mixed support. Lizards from the Liolaemus goetschi group can be found along a wide latitudinal range across Argentina. The group is monophyletic and widely distributed, and therefore provides excellent opportunities to study the evolution of thermal biology. We studied thermal variables of 13 species of the Liolaemus goetschi group, in order to answer three questions. First, are aspects of the thermal biology of the L. goetschi group modelled by the environment or are they evolutionarily conservative? Second, have thermal characteristics of these animals co-evolved? And third, how do the patterns of co-evolution observed within the L. goetschi group compare to those in a taxonomically wider selection of species of Liolaemus? We collected data on 13 focal species and used species information of Liolaemus lizards available in the literature and additional data obtained by the authors. We tackled these questions using both conventional and phylogenetically based analyses. Our results show that lizards from the L. goetschi group and the genus Liolaemus in general vary in critical thermal minimum in relation to mean air temperature, and particularly the L. goetschi group shows that air temperature is associated with critical thermal range, as well as with body temperature. Although the effect of phylogeny cannot be ignored, our results indicate that these thermal biology aspects are modelled by cold environments of Patagonia, while other aspects (preferred body temperature and critical thermal maximum) are more conservative. We found evidence of co-evolutionary patterns between critical thermal minimum and preferred body temperature at both phylogenetic scales (the L. goetschi group and the extended sample of 64 Liolaemus species).","palabras_clave":"THERMOREGULATION|PHYLOGENETIC SCALE|CO-EVOLUTION|COMPARATIVE ANALYSIS|LIOLAEMUS GOETSCHI GROUP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63841,"idioma_id":4,"titulo":"Dynamic mitochondrial-nuclear redistribution of the immunophilin FKBP51 is regulated by PKA signaling pathway to control gene expression in the process of adipocyte differentiation","resumen":"Glucocorticoids play an important role in adipogenesis via the glucocorticoid receptor (GR) that forms a heterocomplex with Hsp90?Hsp70 and one high molecular weight immunophilin FKBP51 or FKBP52. &nbsp;When 3T3-L1 preadipocytes are induced to differentiate, FKBP51 expression progressively increases, whereas FKBP52 decreases, and Hsp90, Hsp70, p23 and Cyp40 remain unchanged. &nbsp;Interestingly, FKBP51 rapidly translocates from mitochondria to the nucleus where it is retained upon its interaction with chromatin and the nuclear matrix. &nbsp;FKBP51 nuclear localization is transient, after 48 h it cycles back to mitochondria. &nbsp;Importantly, this dynamic FKBP51 mitochondrial-nuclear shuttling depends on PKA signaling, since its inhibition by PKI or knock-down of PKA-cá by siRNA, abrogated FKBP51 nuclear translocation induced by IBMX. &nbsp;In addition, FKBP51 electrophoretic pattern of migration is altered by treatment of cells with PKI or knock-down of PKA-cá suggesting that FKBP51 is a PKA substrate. &nbsp;In preadipocytes, FKBP51 co-localizes with PKA-cá in mitochondria. When adipogenesis is triggered, PKA-cá also moves to the nucleus co-localizing with FKBP51 mainly in the nuclear lamina. &nbsp;Moreover, FKBP51 and GR interaction increases when preadipocytes are induced to differentiate. &nbsp;GR transcriptional capacity is reduced when cells are incubated in the presence of IBMX, forskolin or dibutiryl-cAMP, compounds that induced FKBP51 nuclear translocation , but not by an specific activator of EPAC. &nbsp;FKBP51 knock-down facilitates while ectopic expression of FKBP51 blocks adipogenesis. These findings indicate that the dynamic mitochondrial-nuclear shuttling of FKBP51 regulated by PKA may be key in fine tuning the transcriptional control of GR-target genes required for the acquisition of adipocyte phenotype.","palabras_clave":"ADIPOGENESIS|FKBP51|PKA|GLUCOCORTICOID RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63842,"idioma_id":5,"titulo":"La dinámica redistribución mitocondria-núcleo de la inmunofilina FKBP51 es regulada por PKA para modular la expresión de genes durante el proceso de adipogénesis","resumen":"Los glucocorticoides juegan un rol central en la adipogénesis, ejerciendo su acción a través de la unión a su receptor, RG, que se encuentra en el citoplasma formando parte de un heterocomplejo al que pertenece también una inmunofilina (INM) de alto peso molecular FKBP51 o FKBP52. Durante la diferenciación adipocítica los niveles de Hsp90, Hsp70 y p23 no se modifican sin embargo, mientras el nivel de expresión de FKBP52 disminuye el de FKBP51 aumenta progresivamente. FKBP51 sufre una dinámica redistribución mitocondria-núcleo al inicio del proceso de adipogénesis, concentrándose transitoriamente en la lámina nuclear, evento que coincide temporalmente con su reorganización. Esta localización es transitoria, a las 48 hs de iniciado el proceso la INM se concentra nuevamente en las mitocondrias. Esta dinámica redistribución mitocondria -núcleo es regulada por glucocorticoides y, principalmente, por la vía AMPc-PKA ya que la inhibición de PKA por PKI-miristoilado bloquea la traslocación de FKBP51 a núcleo inducida por 3-isobutil-1-metilxantina (IBMX). Se sabe que PKA-c se asocia con RG de manera ligando dependiente potenciando su actividad transcripcional. La capacidad transcripcional de RG disminuye cuando las células son tratadas con IBMX, forskolina o dibutiril-AMPc, agentes que inducen la traslocación a núcleo de FKBP51 y, por lo tanto, PKA podría ejercer un rol dual en la regulación de dicho factor. En síntesis, la presencia de FKBP51 en el núcleo dependiente de la activación de PKA puede ser crítica para el control de RG y posiblemente para otros factores no pertenecientes a la familia de los receptores nucleares cuya función es regulada también por dicha vía de señalización, evento que tiene lugar en una etapa del proceso de diferenciación con alto nivel de remodelamiento de cromatina, pero donde la transcripción debe estar estrictamente controlada para la adquisición del fenotipo adipocítico. ","palabras_clave":"ADIPOGENESIS|FKBP51|PKA|GLUCOCORTICOID RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63843,"idioma_id":4,"titulo":"An efficient and mild access to N-acetyl protected purine nucleosides based on a chemoselective enzymatic hydrolysis","resumen":"N-Monoacetylated derivatives of ribo- (adenosine, guanosine) and 2&#8242;-deoxyribonucleosides (2&#8242;-deoxyadenosine and 2&#8242;-deoxyguanosine), useful as oligonucleotide building blocks, were obtained in 88?100% by enzymatic chemoselective hydrolysis of the corresponding peracetylated nucleosides. Among the tested hydrolases, most satisfactory results were found with acylase I from Aspergillus melleus and Candida antarctica lipase B. For acylase I, the observed chemoselectivity towards ester hydrolysis, without amide reaction, broadens the information about the selectivity of the enzyme and its synthetic applications in the field of nucleosides.","palabras_clave":"CHEMOSELECTIVITY|CANDIDA ANTARCTICA LIPASE B|ACYLASE I|NUCLEOBASE N-PROTECTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63844,"idioma_id":4,"titulo":"Endoparasitic infections in dogs from rural areas in the Lobos District, Buenos Aires province, Argentina","resumen":"Dogs are definite  hosts for several zoonotic helminthes and protozoan. Rural areas from the Lobos  District in the northeast of Buenos Aires province, Argentina, are mainly used  for livestock activity, increasing in this way the number of dogs on farms as well  as the human risk of parasitic infections. The aims of this research were to  evaluate the endoparasitic infections in dogs from farms in the Lobos District  and analyze their zoonotic importance as well as several risk practices and  habits of the rural population. Forty-two dog fecal samples obtained in 21  farms were analyzed through coproparasitological methods and coproantigen  tests, which resulted in an overall parasite prevalence of 69.05% and 80.95% of  the parasitized farms. The most frequent parasites were Trichuris vulpis and  Eucoleus aerophila (26.19%), Echinococcus granulosus (19.05%), Uncinaria  stenocephala and coccids (14.29%). The analysis of epidemiological files  showed several habits of the rural population considered as risk factors associated  with the presence of fecal samples parasitized and the presence of E.  granulosus on the farms. It is clear that people involved with the farms  studied were exposed to several helminthes that could cause serious diseases  like cystic echinococcosis, which can become an important public health issue  and affect the economy worldwide.","palabras_clave":"Environmental contamination|Endoparasites"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63845,"idioma_id":4,"titulo":"CHARACTERIZATION OF CITRUS SINENSIS TRANSCRIPTION FACTORS CLOSELY ASSOCIATED WITH THE NON-HOST RESPONSE TO XANTHOMONAS CAMPESTRIS PV. VESICATORIA","resumen":"Plants, when exposed to certain pathogens, may display a form of genotype-independent resistance, known as non-host response. In this study, the response of Citrus sinensis (sweet orange) leaves to Xanthomonas campestris pv. vesicatoria (Xcv), a pepper and tomato pathogenic bacterium, was analyzed through biochemical assays and cDNA microarray hybridization and compared with Asiatic citrus canker infection caused by Xanthomonas citri subsp. citri. Citrus leaves exposed to the non-host bacterium Xcv showed hypersensitive response (HR) symptoms (cell death), a defense mechanism common in plants but poorly understood in citrus. The HR response was accompanied by differentially expressed genes that are associated with biotic stress and cell death. Moreover, 58 transcription factors (TFs) were differentially regulated by Xcv in citrus leaves, including 26 TFs from the stress-associated families AP2-EREBP, bZip, Myb and WRKY. Remarkably, in silico analysis of the distribution of expressed sequence tags revealed that 10 of the 58 TFs, belonging to C2C2-GATA, C2H2, CCAAT, HSF, NAC and WRKY gene families, were specifically over-represented in citrus stress cDNA libraries. This study identified candidate TF genes for the regulation of key steps during the citrus non-host HR. Furthermore, these TFs might be useful in future strategies of molecular breeding for citrus disease resistance.","palabras_clave":"CITRUS SINENSIS;|MICROARRAY TRANSCRIPTOME ANALYSIS|NON-HOST RESPONSE|XANTHOMONAS CAMPESTRIS PV. VESICATORIA;|XANTHOMONAS CITRI SUBSP. CITRI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63846,"idioma_id":4,"titulo":"Orographic effects related to deep convection events over the Andes region","resumen":"In this work, we analyze a set of 39 storms which took place between 2006 and 2011 over the  South of Mendoza, Argentina. This is a semiarid region situated at mid-latitudes (roughly  between 32S and 36S) at the east of the highest Andes tops which constitutes a natural  laboratory where diverse sources of gravity waves usually take place. We consider a cultivated  subregion near San Rafael district, where every summer a systematic generation of deep  convection events is registered. We propose that the lift mechanism required to raise a parcel  to its level of free convection is partially supplied by mountain waves (MWs). From Weather  Research and Forecasting (WRF) mesoscale model simulations and radar network data, we  calculate the evolution of convective available potential energy and convective inhibition  indices during the development of each storm. Global Final Analysis is used to construct initial  and boundary conditions. Convective inhibition indices are compared with the vertical kinetic  energy capable of being supplied by the MWs, in order to provide a rough estimation of  this possible triggering mechanism. Vertical velocity is chosen as an appropriate dynamical  variable to evidence the presence of MWs in the vicinity of each detected first radar echo. After  establishing a criterion based on a previous work to represent MWs, the 39 storms are split  into two subsets: with and without the presence of MWs. 12 cases with considerable MWs  amplitude are retained and considered. Radar data differences between the two samples are  analyzed and the simulated MWs are characterized.","palabras_clave":"mountain waves|storms|Andes|Mendoza"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63847,"idioma_id":4,"titulo":"A New Approach to Global Gravity Wave Momentum Flux Determination from GPS Radio Occultation Data","resumen":"GPS Radio Occultation (RO) is a well-established technique for obtaining global gravity wave (GW) information. RO uses GPS signals received aboard low Earth orbiting satellites for atmospheric limb sounding. Temperature pro&#64257;les are derived with high vertical resolution and provide a global coverage under any weather conditions o&#64256;ering the possibility for global monitoring of the vertical temperature structure and atmospheric wave parameters. The six satellites constellation COSMIC\/FORMOSAT-3 delivers approximately 2000 temperature pro&#64257;les daily. In this study, we use a method to obtain global distributions of horizontal gravity wave wavelengths, to be applied in the determination of the vertical &#64258;ux of horizontal momentum transported by gravity waves. The horizontal wavenumber is derived by the ratio of the phase shift and the spatial  distance between adjacent temperature &#64258;uctuation pro&#64257;les at a given altitude, following the method by Ern et al. (2004). A new method for the determination of the real horizontal wavelength from triads of vertical pro&#64257;les is presented and applied to the COSMIC data. The horizontal and vertical wavelength, the speci&#64257;c potential energy (Ep) and the vertical &#64258;ux of horizontal momentum (MF) are calculated and their global  distribution is discussed.","palabras_clave":"radio ocultamiento GPS|ondas de gravedad|flujo de momento"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63848,"idioma_id":4,"titulo":"Making rules credible: Divided government and political budget cycles","resumen":"Political budget cycles (PBCs) can result from the credibility problems   office-motivated incumbents face under asymmetric information, due to   the temptation to manipulate fiscal policy to increase their electoral   chances. We analyze the role of rules that limit public debt, because   borrowing is a necessary condition for aggregate PBCs. Since the   legislature must typically authorize new debt, divided government can   make these fiscal rules credible. Commitment is undermined by either   unified government or imperfect compliance with the budget law, which   can help explain why PBCs are stronger in developing countries and in   new democracies. When divided government affects efficiency, voters must   trade off electoral distortions and government competence.","palabras_clave":"POLITICAL BUDGET CYCLES|UNIFIED GOVERNMENT|RULES|CREDIBILITY|DIVIDED GOVERNMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63849,"idioma_id":4,"titulo":"Lysophosphatidic acid increases the production of pivotal mediators of decidualization and vascularization in the rat uterus","resumen":"Introduction  The decidual   reaction and the formation of new vessels in the uterus are two crucial   processes during embryo implantation. Previously, we observed that   lysophosphatidic acid (LPA) increases cyclooxygenase-2 derived ?   prostaglandin E2 production during implantation in the rat uterus and   that it augments the expression of decidualization (IGFBP-1) and   vascularization (IL-10) markers. Both cyclooxygenase and nitric oxide   synthase (NOS) are known enzymes involved in these processes. Thus, we   became interested in studying which factors contribute to LPA   receptor-specific role during the decidual and the vascular reaction at   implantation.  Methods  We adopted a pharmacological approach in&nbsp;vitro   incubating the uterus from rats on day 5 of gestation (day of   implantation) with LPA, DGPP (a highly selective antagonist of LPA3, an   LPA receptor) and cyclooxygenase and NOS selective and non-selective   inhibitors. We determined NOS activity, prostaglandin E2 production and   IGFBP-1 and IL-10 expression to evaluate decidualization and   vascularization.  Results  We   observed that LPA augmented the activity of the inducible NOS isoform   through LPA1\/LPA3. Inducible NOS activity participated in the induction   of cyclooxygenase-2\/prostaglandin E2 increase stimulated by LPA. Also,   cyclooxygenase-2 derived prostaglandins mediated LPA-stimulatory action   on NOS activity. Both cyclooxygenase-2 and inducible NOS mediated LPA   effect on IGFBP-1 and IL-10 expression.  Conclusions  These   results suggest the participation of LPA\/LPA3 in the production of   crucial molecules involved in vascularization and decidualization, two   main processes that prepare the uterine milieu for embryo invasion   during implantation.","palabras_clave":"LPA3 RECEPTOR|DECIDUALIZATION|VASCULARIZATION|IMPLANTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63850,"idioma_id":4,"titulo":"Satisfiability Calculus: The Semantic Counterpart of a Proof  Calculus in General Logics","resumen":"Since its introduction by Goguen and Burstall in 1984, the theory of Institutions has been one of the most widely accepted formalizations of abstract model theory.This work was extended by a number of researchers, José Meseguer among them, who described his ideas in the paper General Logics: an abstract framework that complements the model theoretical view of Institutions by defining the notion of proof theory in a categorical way. In this paper we intend to complete this picture by providing the notion of Satisfiability Calculus, which might be thought of as the semantical counterpart of the notion of Proof Calculus; it provides the formal foundations for those proof systems that use model construction techniques to prove or disprove a given formula, thus, providing a concrete ``implementation'' of the satisfiability relation of an institution.","palabras_clave":"Software Specification|Category Theory|Automatic Deduction|Logic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63851,"idioma_id":4,"titulo":"ALKALOID PROFILING AND ANTICHOLINESTERASE ACTIVITY OF SOUTH AMERICAN Lycopodiaceae SPECIES","resumen":"  The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for theirin vitro anticholinesterase activities. IC50 values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 ìg\/mL), followed by H. quadrifariata (2.0 ± 0.3 ìg\/mL), H. acerosa (5.5 ± 0.9 ìg\/mL), H. heterocarpon (25.6 ± 2.7 ìg\/mL) and L. cernua (42.6 ± 1.5 ìg\/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 ìg\/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.","palabras_clave":"HUPERZIA ACEROSA|HUPERZIA HETEROCARPON|HUPERZIA QUADRIFARIATA|HUPERZIA REFLEXA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63852,"idioma_id":4,"titulo":"Study of the interaction of Huperzia saururus Lycopodium alkaloids with the acetylcholinesterase enzyme","resumen":"In the present study, we describe and compare the binding modes of three Lycopodium alkaloids (sauroine, 6-hydroxylycopodine and sauroxine; isolated from Huperzia saururus) and huperzine A with the enzyme acetylcholinesterase. Refinement and rescoring of the docking poses (obtained with different programs) with an all atom force field helped to improve the quality of the protein?ligand complexes. Molecular dynamics simulations were performed to investigate the complexes and the alkaloid´s binding modes. The combination of the latter two methodologies indicated that binding in the active site is favored for the active compounds. On the other hand, similar binding energies in both the active and the peripheral sites were obtained for sauroine, thus explaining its experimentally determined lack of activity. MM-GBSA predicted the order of binding energies in agreement with the experimental IC50 values","palabras_clave":"ACETYLCHOLINESTERASA|BINDING MODES|ALKALOIDS|MOLECULAR SIMULATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63853,"idioma_id":5,"titulo":"Reflexiones teóricas, herramientas metodológicas y panoramas empíricos para una cartografía de la biopolítica contemporánea","resumen":"Se repasan las políticas vitales que se despliegan con el avance del siglo XXI, por lo cual revisten aún un carácter emergente. Éstas se caracterizan por ser políticas de la vida misma, que apelan a la capacidad creciente de los individuos 'de controlar, administrar, modificar, redefinir y modular las propias capacidades vitales de los seres humanos en cuanto criaturas vivas' (Rose, 2012: 25). Y se diferencian de otra forma histórica, la política de la salud, que abarcó desde el siglo XVIII hasta la primera mitad del siglo XX, y se ocupó (frecuentemente de manera coercitiva) de la salud de la población.  Se retoma el andamiaje teórico-metodológico foucaulteano, para generar categorías y herramientas teórico-operativas noveles que den cuenta de la también novel problemática, desgranando categorías como individualidad somática, susceptibilidad, responsabilidad genética, ciudadanía biológica, biosocialidad, yo neuroquímico, biovalor, ética somática, entre otras que configuran un prolífico entramado argumentativo. También se presenta una serie de conceptos operativos, como cartografía del presente, molecularización, estilo de pensamiento, aparato de verdad y bioeconomía.  En esta apuesta quedan expuestos los límites del proyecto de Foucault, ya sea porque en sus desplazamientos, reconfiguraciones y recentramientos analíticos, se acuñen o recurra a nuevos conceptos, como porque las lógicas que concibió el pensador francés ya no resultan adecuadas para describir los procesos que se suscitan.","palabras_clave":"biopolítica|cartografía|biomedicina|poder"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63854,"idioma_id":4,"titulo":"Glibenclamide inhibits cell growth by inducing G0\/G1 arrest in the human breast cancer cell line MDA-MB-231","resumen":"Background: Glibenclamide (Gli) binds to the sulphonylurea receptor (SUR) that is a regulatory subunit of ATP-sensitive potassium channels (KATP channels). Binding of Gli to SUR produces the closure of KATP channels and the inhibition of their activity. This drug is widely used for treatment of type 2-diabetes and it has been signaled as antiproliferative in several tumor cell lines. In previous experiments we demonstrated the antitumoral effect of Gli in mammary tumors induced in rats. The aim of the present work was to investigate the effect of Gli on MDA-MB-231 breast cancer cell proliferation and to examine the possible pathways involved in this action. Results: The mRNA expression of the different subunits that compose the KATP channels was evaluated in MDA-MB-231 cells by reverse transcriptase-polymerase chain reaction. Results showed the expression of mRNA for both pore-forming isoforms Kir6.1 and Kir6.2 and for the regulatory isoform SUR2B. Gli inhibited cell proliferation assessed by a clonogenic method in a dose dependent manner, with an increment in the population doubling time. The KATP channel opener minoxidil increased clonogenic proliferation, effect that was counteracted by Gli. When cell cycle analysis was performed by flow cytometry, Gli induced a significant cell-cycle arrest in G0\/G1 phase, together with an up-regulation of p27 levels and a diminution in cyclin E expression, both evaluated by immunoblot. However, neither differentiation evaluated by neutral lipid accumulation nor apoptosis assessed by different methodologies were detected. The cytostatic, non toxic effect on cell proliferation was confirmed by removal of the drug. Combination treatment of Gli with tamoxifen or doxorubicin showed an increment in the antiproliferative effect only for doxorubicin.Conclusions: Our data clearly demonstrated a cytostatic effect of Gli in MDA-MB-231 cells that may be mediated through KATP channels, associated to the inhibition of the G1-S phase progression. In addition, an interesting observation about the effect of the combination of Gli with doxorubicin leads to future research for a potential novel role for Gli as an adjuvant in breast cancer treatment","palabras_clave":"Glibenclamide|Potassium channels|MDA-MB-231|Cytostatic effect"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63855,"idioma_id":5,"titulo":"Garrapatas (ixodida) y ácaros (mesostigmata) parásitos de roedores sigmodontinos del Delta del Paraná, Argentina","resumen":"Se determinaron las especies de garrapatas y ácaros mesostigmata parásitos de roedores sigmodontinos en una localidad del Delta del Paraná. Fueron registradas 38 asociaciones parásito-hospedador entre dos especies de garrapatas y cinco especies de ácaros con seis especies de roedores, que mostraron una distribución agregada de sus parásitos. Dos de las asociaciones (Holochilus brasiliensis-Amblyomma triste y H. brasiliensis-Ixodes loricatus) son nóveles, siendo la asociación H. brasiliensis-Am. triste de importancia sanitaria ya que esta garrapata es vector de Rickettsia parkeri, patógeno que afecta a los humanos. La mayoría de las asociaciones se detectaron previamente sólo en la eco-región correspondiente a la localidad de estudio. Por el contrario, unas pocas asociaciones ya registraban antecedentes para otras regiones argentinas, el Brasil, Paraguay o el Uruguay.","palabras_clave":"GARRAPATAS|ACAROS|ROEDORES|ASOCIACIONES|PARANÁ"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63856,"idioma_id":4,"titulo":"A fraction rich in phenyl propanoids from L. divaricata aqueous extract is capable of inducing apoptosis, in relation to H2O2 modulation, on a murine lymphoma cell line","resumen":"Leukemia and lymphoma are a group of heterogeneous neoplastic disorder of white blood cells characterized by the uncontrolled proliferation and block in differentiation of hematopoietic cells. Nowadays, there is an interest in therapy with drugs of plant origin because conventional medicine can be inefficient or also results in side effects. Larrea divaricata Cav, is a plant widely distributed in Argentina that possess antiproliferative and antioxidant activities reported. Nordihydroguaiaretic acid (NDGA) was previously found in the plant and related to both antiproliferative and pro-proliferative action on a lymphoma cell line. In order to demonstrate whether the presence of NDGA may be beneficial or not in the antiproliferative action of the aqueous extract, the extract of L. divaricata was submitted to a fractionation and fractions with and without NDGA were studied in a murine lymphocitic leukaemia cell line (EL-4) proliferation. The effect of the most active fraction was studied in relation to H2O2 modulation and the synergistic action between compounds, found in fractions, was analyzed. The presence of NDGA was not a detonator for pro-proliferative action and its presence could be beneficial in low concentrations allowing a synergist antiproliferative action with other compounds.","palabras_clave":"LARREA DIVARICATA|APOPTOSIS|LYMPHOMA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63857,"idioma_id":4,"titulo":"Methyl anthranilate as generator and quencher of reactive oxygen species. A photochemical study","resumen":"Methyl anthranilate (MA) is a naturally occurring aromatizer and flavorant agent  frequently  employed in  food industry and in personal care products. Due to the property of MA of being a UV-A radiation- absorber it is incorporated to topical sunscreen formulations.  Within the framework of the wide variety of practical uses and applications, the photodegradation of MA  becomes a topic of interest from health to an economic  point of view. In this context, kinetics and mechanistic aspects of its photo-oxidation has been studied,  in order to know the  degradation pathways of MA under natural-like photoirradiation. The assays were performed in the absence and in the presence of riboflavin (Rf, vitamin B2) acting the later as a native photosensitizer. The results showed that MA is capable to generate singlet molecular oxygen (O2(1&#916;g)) upon direct UV-A irradiation, with a quantum yield value of 0.13 ± 0.02. The anthranilate also quenches the oxidative species with an overall rate constant value kt = 2.1 ± 0.2 x107  M-1s-1 while the reactive interaction between MA and O2(1&#61508;g) showed a rate constant value in the range of 5.9 ? 6.8  x106  M-1s-1. The kr\/kt ratio, lower than 0.35 indicates that only one third of the  O2(1&#61508;g)-quenching events effectively lead to oxidation of the scavenger, being this an acceptable property for a sunscreen component. Regarding the Rf-photosensitized visible-light irradiation of MA-Rf mixtures, the anthranilate  quenches the electronically excited singlet and triplet states of the vitamin. As a result the oxidative species O2?? and H2O2, are generated, being the later effectively deactivated by MA. At first, the described results would show MA as a candidate for  UV-A and visible-light mediated degradation. Nevertheless, at the same time MA acts as a moderate physical scavenger of photogenerated oxidative species in a sort of self-protector against photoinduced degradation and protector of oxidizable surrounding compounds.","palabras_clave":"Methyl anthranilate|Singlet Oxygen|Reactive Oxygen Species|photochemistry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63858,"idioma_id":4,"titulo":"Frustration behaviors in domestic dogs","resumen":"During extinction a previously learned behavior stops being reinforced. In addition  to the decrease in the rate of the instrumental response, it produces an aversive  emotional state known as frustration. This state can be assimilated with the fear  reactions that occur after aversive stimuli are introduced at both the physiological  and behavioral levels. This study evaluated frustration reactions of domestic dogs  (Canis familiaris) during a communicative situation involving interactions with a  human. The task included the reinforcement and extinction of the gaze response  toward the experimenter?s face when the dogs tried to obtain inaccessible food. The  dog?s frustration reactions during extinction involved an increase in withdrawal and  side orientation to the location of the human as well as lying down, ambulation,  sniffing, and vocalizations compared with the last acquisition trial. These results  are especially relevant for domestic dog training situations in which the extinction  technique is commonly used to discourage undesirable behaviors","palabras_clave":"DOMESTIC DOGS|FRUSTRATION|EXTINCTION|COMMUNICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63859,"idioma_id":5,"titulo":"Comprensión de textos en niños: Intervenciones orientadas a su desarrollo en contextos desfavorables","resumen":"La comprensión de textos es una de las habilidades cognitivas más complejas que poseen los seres humanos. El proceso de comprender un texto implica una construcción activa de significado mediante la puesta en marcha simultánea de habilidades lingüísticas, memoria, atención, razonamiento, conocimiento acerca del mundo y de las estrategias de lectura, entre otros. El objetivo de este trabajo es comparar el efecto de una intervención sobre las capacidades de comprensión lectora en niños de 10 años con necesidades básicas insatisfechas (NBI) y satisfechas (NBS). Para ello a ambos grupos se les evaluó su capacidad de comprensión de textos antes y después de la intervención mediante el Test Leer para Comprender, utilizando específicamente dos áreas: modelos mentales y flexibilidad. Los niños recibieron una sesión con un breve entrenamiento para favorecer el desarrollo de estas habilidades. Los resultados mostraron que en la primera evaluación, los niños NBI presentaron menos cantidad de respuestas correctas que los alumnos NBS en el área de flexibilidad. Las actividades de una sola sesión de entrenamiento no afectaron el desempeño de los niños NBS en ninguna de las dos habilidades cognitivas. Sin embargo, la segunda evaluación mostró que el programa produjo un aumento significativo en las respuestas correctas de los niños NBI en el área de flexibilidad. Estos resultados señalan que una sesión de entrenamiento en la resolución de las actividades seleccionadas, podría mejorar la comprensión de textos, específicamente del área de flexibilidad, en una población con vulnerabilidad social.","palabras_clave":"comprensión lectora|vulnerabilidad social|flexibilidad|modelos mentales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63860,"idioma_id":4,"titulo":"ECTOMYCORRHIZAS NATURALLY ESTABLISHED IN NOTHOFAGUS NERVOSA SEEDLINGS UNDER DIFFERENT CULTIVATION PRACTICES IN A FOREST NURSERY","resumen":"Mycorrhizas are mutualistic associations between  soil fungi and plant roots which usually improve water and  nutrient uptake, influencing plant fitness. Nothofagus nervosa  (Raulí) is an ecologically and economically important species  of South American temperate forests. Since this native tree  species yields valuable timber, it was overexploited and its  natural distribution area was critically reduced, so it is currently  included in domestication and conservation programs.  Among the factors that should be considered in these programs  are the ectomycorrhizas (EcM), which would be important  for the successful establishment and survival of  outplanted seedlings. The aim of this work was to analyze  the abundance and diversity of EcM in N. nervosa nurserycultivated  seedlings assessed by morphotyping, fungal isolation,  and DNA sequencing. Arbuscular mycorrhiza (AM)  occurrence was also studied. A 2-year trial was conducted  following the cultivation conditions used for domestication  programs. Seedlings were cultivated under two different cultivation  practices (greenhouse and nursery soil) without artificial  inoculation of mycorrhizal fungi. Seedlings? roots were  examined at different times. It was observed that they developed  EcM between 6 and 12 months after germination and  AMs were not detected in any plant. The most abundant  ectomycorrhizal fungi present in seedlings? roots were  Tomentella ellisii (Basidiomycota) and an unidentified fungus  named Ascomicetous EcM sp. 1. Abundance and diversity of  EcM varied between the two cultivation techniques analyzed  in this study, since seedlings that continued growing in the  greenhouse had higher colonization values, but those  transplanted to the nursery soil were colonized by a higher  diversity of fungal taxa.","palabras_clave":"RAULI|TREE DOMESTICATION|CONTAINERIZED SEEDLINGS, FERTIRIGATION|REFORESTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63861,"idioma_id":4,"titulo":"Disentangling Fabiana imbricata (Solanaceae) regeneration: The importance of disturbance and rainfall","resumen":"In Mediterranean ecosystems, fire influences plant population dynamics and changes plant communitystructure by affecting germination and establishment of seeder shrubs. Fabiana imbricata is a long-lived  seeder shrub with a broad distribution in South America. In Northwestern Patagonia grasslands there are many records of F. imbricata recruitment after fires.We hypothesized that recruitment is promoted by: 1)soil erosion that exposes deeply buried seeds; 2) direct fire effects such as heat that could break seed dormancy; 3) indirect fire effects (i.e. increase of light availability and elimination of allelopathic substances)and 4) water availability in spring. In field conditions, we tested the effects of shading and soildisturbance on F. imbricata recruitment and seed availability and distribution in the soil profile. Under controlled conditions, we investigated the influence of leaf leachate, light, heat and water on seed germination and seedling emergence. Seed germination was inhibited by leachate, and seedling emergence was inhibited by seed heated to 80 C and 120 for 5 min. F. imbricata seedlings only emerged in germination treatments that simulated wet and very wet spring conditions. Fire and postfire wind that  favor seed exposure, combined with high precipitation early in the growing season, would be necessary for successful shrub recruitment. When F. imbricata shrubland colonizes the grassland, it strongly  modifies local vegetation structure, reduces species richness and increases fuel loads. F. imbricata is a key species in the ecosystem specially related to fire regime. Information on the postfire regeneration of this species would provide valuable knowledge of the changes in biomass accumulation in a fire-prone Mediterranean ecosystem.","palabras_clave":"FIRE|GERMINATION REQUIREMENTS|MEDITERRANEAN GRASSLAND|PATAGONIA|SEEDER SHRUB"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63862,"idioma_id":4,"titulo":"A convenient synthesis of a 2,7-difunctional tetra(alkoxy)triphenylene involving 4,4-diacetoxy-3,3dialkoxybiphenyl as a key precursor and its conversion to extended hybrid mesogenic compounds","resumen":"A new rational pathway to 2,7-difunctionalized-Beta-hexasubstituted triphenylenes is presented, requiring less protection\/deprotection and purification steps than more conventional synthetic procedures in the framework of the 'biphenyl route'. Main improvements are deprotection via alkaline hydrolysis of an ester in ethanol\/water medium instead of using toxic and pyrophoric reagents like lithium diphenylphosphide, and the use of easily prepared brominated precursors instead of iodinated reagents for biphenyl synthesis. 4,4´-Diacetoxy-3,3´-bis(hexyloxy)biphenyl has been synthesized under this scheme, and characterized by 1H NMR, elemental analysis and single-crystal crystallography. It crystallizes in the P-1 space group, and exhibits a layered structure built-up through dipolar, C-H...Pi and C-H...O=C non-covalent interactions. This compound has been oxidatively coupled with 1,2-bis(hexyloxy)benzene to yield 2,7-dihydroxy-3,6,10,11-tetrakis(hexyloxy)triphenylene, a non mesogen key precursor for the synthesis of the corresponding liquid-crystalline 2,7-difunctional triphenylenes. Indeed, a reactive 2,7 difunctional mesogen was prepared and used to produce new triphenylene-siloxane hybrid monomeric, trimeric and polymeric mesogens. All of them exhibited Colh mesophases. ","palabras_clave":"LIQUID CRYSTALS|TRIPHENYLENES|BIPHENYL ROUTE|SILOXANE|POLYMER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63863,"idioma_id":4,"titulo":"Simulation of Mesogenic Diruthenium Tetracarboxylates. Development of a Force Field for Coordination Polymers of the MMX type","resumen":"(Este artículo fue portada de la revista)A classical molecular mechanics force field, able to simulate coordination polymers (CP) based on ruthenium carboxylates (Ru2(O2CReq)4Lax) (eq = equatorial group containing aliphatic chains, Lax¼ axial ligand), has been developed.&nbsp;New parameters extracted from experimental data and quantum calculations&nbsp; on short aliphatic chains model systems were included in the generalized AMBER force field. The proposed parametrization was evaluated using model systems with known structure, containing either short or long aliphatic chains; experimental results were reproduced satisgfactorily. New parameters extracted from experimental data and quantum calculations&nbsp; on short aliphatic chains model systems were included in the generalized AMBER force field. The proposed parametrization was evaluated using model systems with known structure, containing either short or long aliphatic chains; experimental results were reproduced satisgfactorily. This modified force field, although in a preliminary stage, could then be applied to long chain liquid crystalline compounds. The resulting atomistic simulations allowed assessing the relative influence of the factors determining the CP conformation, determinant for the physical properties of these materials. VC 2013 Wiley Periodicals, Inc.","palabras_clave":"FORCE FIELD|RUTHENIUM CARBOXYLATES|ATOMISTIC SIMULATIONS|MMX POLYMERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63864,"idioma_id":4,"titulo":"PARASITIC STAGE OF OSTERTAGIA OSTERTAGI. A MATHEMATICAL MODEL FOR THE LIVESTOCK PRODUCTION REGION OF ARGENTINA","resumen":"The model here presented describes the dynamics of adult Ostertagia Ostertagi parasites in cattle in the field.The model also describes the population of infecting L3 larvae subjet to the conditioning process while in the pasture as well as the inhibition-desinhibition process in ingested L4 larvae. The model has two modules: one for the conditioning processand the other for the parasitic stage.The first module determines the proportion of ingested L3 larvae that will go through an arrested stage of development,or hypobiosis,and for how long depending on the weather conditions and the length of the period of exposure in the pasture.The second one describes the adult population dynamics as deinhibiting larvae and recently ingested larvae accumulate in the animal.The model has a discrete daily step and is parameterized using fuzzy inference system of the Mamdani type (FM).Given that the inhibition-desinhibition process is not yet fully understood,a series of perturbations on the differents input variables were performed with the purpose of analysisng the model´s behavior.Simulations show that the adult population exhibits two maximal peaks of infection in the hosts in December-January (Summer) and April-May (Autumn).This high values are a consequence of hypobitic larvae resuming their development at those times.","palabras_clave":"FUZZY RULE BASED SYSTEMS|GASTROINTESTINAL NEMATODES|INHIBITION-DISINHIBITION|POPULATION DYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63865,"idioma_id":5,"titulo":"'Diálogos entre feminismos y Buen Vivir\/ Vivir Bien: Distintas perspectivas, múltiples puntos de encuentro'","resumen":"Este artículo surgedel diálogo entre distintos saberes y conocimientos, con el objetivo deidentificar principalmente las convergencias entre el Buen Vivir\/VivirBien y los feminismos latinoamericanos. Iniciamos este trabajo considerando lasideas centrales del enfoque del Buen Vivir\/Vivir Bien y sus mecanismos detraducción concreta en las reformas constitucionales de Bolivia y Ecuador.Luego, nos detenemos en las confluencias -aunque identificando algunastensiones- entre esta última propuesta y los feminismos, haciendo foco en ladimensión económica. Y finalmente, reflexionamos sobre los aspectos nodalesdesarrollados en el trabajo, buscando, asimismo repensar el papel las cienciassociales a la luz de estas corrientes de pensamiento y de los procesos socialescontrahegemónicos.","palabras_clave":"BUEN VIVIR|FEMINISMO LATINOAMERICANO|DESARROLLO|PERSPECTIVAS DECOLONIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63866,"idioma_id":4,"titulo":"Aripiprazole salts. III. Bis(aripiprazolium) oxalate-oxalic acid (1\/1)","resumen":"The asymmetric unit of the title salt [systematic name: bis­(4-(2,3-dichloro­phen­yl)-1-{4-[(2-oxo-1,2,3,4-tetra­hydro­quinolin-7-yl)­oxy]but­yl}piperazin-1-ium) oxalate-oxalic acid (1\/1)], 2C23H28Cl2N3O2+·C2O42-·C2H2O4, consists of one protonated aripiprazole unit (HArip+), half an oxalate dianion and half an oxalic acid mol­ecule, the latter two lying on inversion centres. The conformation of the HArip+ cation differs from that in other reported salts and resembles more the conformation of neutral Arip units in reported polymorphs and solvates. The inter­molecular inter­action linking HArip+ cations is also similar to those in reported Arip compounds crystallizing in the space group Poverline{1}, with head-to-head N-H...O hydrogen bonds generating centrosymmetric dimers, which are further organized into planar ribbons parallel to (01overline{2}). The oxalate anions and oxalic acid mol­ecules form hydrogen-bonded chains running along [010], which `pierce´ the planar ribbons, inter­acting with them through a number of stronger N-H...O and weaker C-H...O hydrogen bonds, forming a three-dimensional network.","palabras_clave":"ARIPIPRAZOLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63867,"idioma_id":4,"titulo":"Solid state modifications of drospirenone","resumen":"This manuscript presents a structural, thermal and spectroscopic characterization of two different solid state forms of drospirenone (C24 H30 O3), a crystalline phase and an amorphous one. The molecule contains three fused six-membered rings and two five-membered rings; none of them is planar in character.An intra-molecular hydrogen bond was observed in the crystalline phase. Although the melting point is 202(1) C, only very weak interactions could be considered responsible for the packing in the crystal. The amorphous phase was obtained from molten material and was found five times more soluble than the crystalline one. Infrared and Raman spectra for both phases were recorded and the vibrational properties were calculated using the GAUSSIAN 03 software package; overall agreement between calculations and experiment was obtained.","palabras_clave":"Drospirenona|Crystal structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63868,"idioma_id":4,"titulo":"Insulin receptor membrane retention by a traceable chimeric mutant","resumen":"Background: The insulin receptor (IR) regulates glucose homeostasis, cell growth and differentiation. It has beenhypothesized that the specific signaling characteristics of IR are in part determined by ligand-receptor complexeslocalization. Downstream signaling could be triggered from the plasma membrane or from endosomes. Regulationof activated receptor's internalization has been proposed as the mechanism responsible for the differential isoformand ligand-specific signaling.Results: We generated a traceable IR chimera that allows the labeling of the receptor at the cell surface. Thismutant binds insulin but fails to get activated and internalized. However, the mutant heterodimerizes with wildtype IR inhibiting its auto-phosphorylation and blocking its internalization. IR membrane retention attenuates AP-1transcriptional activation favoring Akt activation.Conclusions: These results suggest that the mutant acts as a selective dominant negative blocking IRinternalization-mediated signaling.Keywords: Insulin receptor, Membrane retention, Dominant negative, Endocytosis","palabras_clave":"Insulin|Insulin receptor|Endocytosis|Membrane retention"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63869,"idioma_id":4,"titulo":"Adlayers of alkanedithiols on Au(111): effect of disulfide reducing agent","resumen":"High-resolution photoemission spectroscopy is used to characterize   adlayers of ethane-, hexane-, and nonanedithiol molecules grown on   Au(111) surfaces by the immersion method. The effect of using a reducing   agent during and after the immersion to inhibit or eliminate S-S bonds   is investigated. Our results demonstrate that immersion 24 h in   millimolar dithiol ethanolic solutions gives rise to the formation of   multilayers; this effect is more pronounced in the case of   ethanedithiol, the shortest molecule. A post-treatment with a disulfide   reducing agent is effective to produce monolayers of standing-up   molecules; this effect is again more pronounced in the case of   ethanedithiol. Finally, the immersion 24 h in a solution containing   dithiol and the reducing agent gives an unexpected result: most   molecules remain adsorbed in the lying-down configuration; in this case,   the almost complete suppression of the standing-up phase occurs equally   with the three types of molecules, which suggests that the formation of   S-S bonds must be important for the lifting of the molecule","palabras_clave":"Alkanes|Disulfides|Gold|Reducing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63870,"idioma_id":4,"titulo":"Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection","resumen":"Previously we showed that orally administered Lactobacillus rhamnosus CRL1505 beneficially regulated the balance between pro- and anti-inflammatory mediators in the lungs of poly(I:C)-challenged mice, allowing an effective inflammatory response against the TLR3\/RIG-I agonist but at the same time reducing tissue damage. The aim of the present study was to investigate whether oral administration of the CRL1505 strain was able to improve resistance against respiratory syncytial virus (RSV) infection in infant mice and to evaluate the immunological mechanisms involved in the immunobiotic effect. We demonstrated that treatment of 3-week old BALB\/c mice with L. rhamnosus CRL1505 significantly reduce lung viral loads and tissue injuries after the challenge with RSV. Moreover, we showed that the protective effect achieved by the CRL1505 strain is related to its capacity to differentially modulate respiratory antiviral immune response. Our results shows that IFN-&#947; and IL-10 secreted in response to L. rhamnosus CRL1505 oral stimulation would modulate the pulmonary innate immune microenvironment conducting to the activation of CD103+ and CD11bhigh dendritic cells and the generation of CD3+CD4+IFN-&#947;+ Th1 cells with the consequent attenuation of the strong and damaging Th2 reactions associated with RSV challenge. Our results indicate that modulation of the common mucosal immune system by immunobiotics could favor protective immunity against respiratory viral pathogens with a high attack rate in early infancy, such as RSV.","palabras_clave":"L. rhamnosus CRL1505|respiratory syncytial virus|antiviral immunity|respiratory tract"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63871,"idioma_id":4,"titulo":"Synthesizing Masking Fault-Tolerant Systems from Deontic Specifications","resumen":"In this paper, we study the problem of synthesizing fault-tolerant components from specifications, i.e., the problem of automatically constructing a fault-tolerant component implementation from a logical specification of the component, and the system?s required level of fault-tolerance. We study a specific level of fault-tolerance: masking tolerance. A system exhibits masking tolerance when both the liveness and the safety properties of the behaviors of the system are preserved under the occurrence of faults. In our approach, the logical specification of components is given in dCTL, a branching time temporal logic with deontic operators, especially designed for fault-tolerant component specification. The synthesis algorithm takes the component specification, and automatically determines whether a component with masking fault-tolerance is realizable, and the maximal set of faults supported for this level of tolerance. Our technique for synthesis is based on capturing masking fault-tolerance via a simulation relation. Furthermore, a combination of an extension of a synthesis algorithm for CTL to cope with dCTL specifications, with simulation algorithms, is defined in order to synthesize masking fault-tolerant implementations.","palabras_clave":"Síntesis de Programas|Métodos Formales|Verificación de Programas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63872,"idioma_id":4,"titulo":"Training Planning and Working Memory in Third Graders","resumen":"  p, li { white-space: pre-wrap; }    Working memory and planning are fundamental cognitive skills supporting fluid reasoning. We show that 2 games that train working memory and planning skills in school-aged children promote transfer to 2 different tasks: an attentional test and a fluid reasoning test. We also show long-term improvement of planning and memory capacities in 8-year-old children after playing adaptive computer games specifically tailored to entrain these cognitive functions. Working memory capacity expanded from 5 to 7 items by using our games. Furthermore, steady progression in the task indicates that this capacity can be trained rapidly. Planning abilities persisted in a nonmarkovian form of play,where a move is highly influenced by previous moves, avoiding back-ups. Here, we introduce a public and growing platform (http:\/\/www.matemarote.com.ar\/) developed for this research which has the potential for wide use in educational research.","palabras_clave":"Working Memory|Cognitive Skill Transfer|Planning|Third Graders"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63873,"idioma_id":5,"titulo":"Respuesta instrumental del gravímetro Scintrex CG-5 #40484 en modos continuo y relevamiento","resumen":"Debido a sus características técnicas y su simplicidad de medición, el gravímetro automático Scintrex CG-5 es usado por una variada gama de usuarios. Diversas investigaciones han demostrado que el desempeño de cada gravímetro debe ser evaluado particularmente, puesto que la respuesta de cada instrumento es afectada de forma diferente por factores externos, dando lugar a residuos que podrían ser del orden de la precisión demandada. En este trabajo, para el gravímetro Scintrex CG-5 S\/N 40484: 1) Se investigó en condiciones de laboratorio para el modo continuo, la influencia sobre los registros gravimétricos de variables meteorológicas (presión y temperatura atmosféricas). También se determinó la deriva instrumental de largo plazo (registros de 35 días). 2) En el modo relevamiento se llevó a cabo una calibración, una determinación de gradiente vertical de gravedad local y mediciones sobre puntos situados en la provincia de San Juan. Las diferencias de gravedad entre estaciones y los parámetros del gravímetro fueron estimadas mediante un ajuste de red.En base a nuestros resultados, concluimos que este instrumento podría detectar en modo continuo (e.g., movilidad cortical, hidrogeología, etc.) o en modo relevamiento, variaciones de gravedad con exactitudes de algunos microgales. En ambos casos es necesario seguir un adecuado protocolo de medición y postprocesamiento de datos.","palabras_clave":"MICROGRAVIMETRIA|GRAVIMETROS|AUTOMATICOS|RELATIVA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63874,"idioma_id":4,"titulo":"Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil","resumen":"A phenanthrene-degrading consortium (CON-Phe) was obtained from a chronically contaminated soil. The consortium degraded 58% of the phenanthrene supplied during the first 7 days of incubation with the concomitant accumulation of 1-hydroxy-2-naphthoic acid (HNA). The composition of CON-Phe and its dynamic during phenanthrene degradation were determined using culture-dependent and independent approaches (polymerase chain reaction?denaturing gradient gel electrophoresis and clone libraries). Among the detected bacteria by both methods, Sphingomonadaceae family frequently occurred, but some genera were only observed through culture-dependent methods (Enterobacter sp. and Pseudomonas sp.) and others only through culture-independent methods (Ochrobacterium sp., Alcaligenes sp.). Five different strains were isolated and identified; between them only the strain AM (Sphingobium sp.) showed phenanthrene degradation. And only in strains AM and B (Enterobacter sp) evidence of the presence of PAH-ring hydroxylating dioxygenases genes was found. In order to determine the role of the isolated strains in the CON-Phe and the interaction between them, the phenanthrene degradation by defined mixed cultures was studied. All the defined consortia, formed by strain AM together with another of the isolated strains, showed percentages of phenanthrene biodegradation significantly higher than the strain AM alone and the natural consortia. In addition, no accumulation of HNA was observed in these defined consortia. These results might suggest that in the soil consortia the competition between the species and the community dynamics could cause a negative effect in the phenanthrene degradation. On the other hand, a synergistic effect between the phenanthrene-degrading strain AM and the other isolated strains was observed.","palabras_clave":"Phenantrene degradation|Bacterial consortium|DGGE|Sphingomonadaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63875,"idioma_id":4,"titulo":"Near-infrared photometry of Galactic planetary nebulae with the VVV Survey","resumen":"Context. Planetary nebulae (PNe) are powerful tracers of evolved stellar populations. Among the 3000 known PNe in the Galaxy,about 600 are located within the 520 square-degree area covered by the VVV survey. The VVV photometric catalogue provides animportant new dataset for the study of PNe, with high-resolution imaging in five near-infrared bands.Aims. There are various colour-colour diagrams that can be obtained from the VVV filters. We investigate the location of PNe in thesediagrams and the separation from other types of objects. This includes the new Y&#8722;J vs. Z&#8722;Y diagram.Methods. Aperture photometry of known PNe in the VVV area was retrieved from source catalogues. Care was taken to minimiseany confusion with field stars. The colours of the PNe we are determined for (H&#8722;Ks ), (J&#8722;H), (Z&#8722;Y), and (Y&#8722;J), and compared to starsand to other types of emission line objects. Cloudy photo-ionisation models were used to predict colours for typical PNe.Results. We present near-infrared photometry for 353 known PNe. The best separation from other objects is obtained in the (H&#8722;Ks )vs. (J&#8722;H) diagram. We calculated the emission-line contribution to the in-band flux based on a model for NGC 6720: we find thatthis is highest in the Z and Y bands at over 50%, lower in the J band at 40%, and lowest in the H and Ks bands at 20%. A new viewof PNe in the wavelength domain of the Z and Y bands is shown. Photo-ionisation models are used to explore the observed coloursin these bands. The Y band is shown to be dominated by He i 1.083 &#956;m and He ii 1.012 &#956;m, and colours involving this band are verysensitive to the temperature of the ionizing star.Conclusions. The VVV survey represents a unique dataset for studying crowded and obscured regions in the Galactic plane. Thediagnostic diagrams presented here allow one to study the properties of known PNe and to uncover objects not previously classified.","palabras_clave":"planetary nebulae: general|infrared: ISM|catalogs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63876,"idioma_id":4,"titulo":"Zinc co-ordination by the DHHC cysteine-rich domain of the palmitoyltransferase Swf1","resumen":"S-acylation, commonly known as palmitoylation, is a widespread  post-translational modification of proteins that consists of  the thioesterification of one or more cysteine residues with  fatty acids. This modification is catalysed by a family of  PATs (palmitoyltransferases), characterized by the presence  of a 50-residue long DHHC-CRD (Asp-His-His-Cys cysteinerich  domain). To gain knowledge on the structure?function  relationships of these proteins, we carried out a randommutagenesis  assay designed to uncover essential amino acids in  Swf1, the yeast PAT responsible for the palmitoylation of SNARE  (soluble N-ethylmaleimide-sensitive fusion protein-attachment  protein receptor) proteins.We identified 21 novel loss-of-function  mutations, which are mostly localized within the DHHC-CRD.  Modelling of the tertiary structure of the Swf1 DHHC domain  suggests that it could fold as a zinc-finger domain, co-ordinating  two zinc atoms in a CCHC arrangement. All residues predicted to  be involved in the co-ordination of zinc were found to be essential  for Swf1 function in the screen. Moreover, these mutations result  in unstable proteins, in agreement with a structural role for  these zinc fingers. The conservation of amino acids predicted to  form each zinc-binding pocket suggests a shared function, as the  selective pressure to maintain them is lost upon mutation of one  of them. A Swf1 orthologue that lacks one of the zinc-binding  pockets is able to complement a yeast swf1&#916; strain, possibly  because a similar fold can be stabilized by hydrogen bonds instead  of zinc co-ordination. Finally, we show directly that recombinant  Swf1 DHHC-CRD is able to bind zinc. Sequence analyses of  DHHC domains allowed us to present models of the zinc-binding  properties for all PATs.","palabras_clave":"PALMITOYLATION |S-ACYLATION|YEAST|SNARES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63877,"idioma_id":4,"titulo":"Pistachio (Pistacia vera var Kerman) from Argentinean cultivars. A natural product with potential to improve human health","resumen":"ABSTRACT  The chemical profile, mineral content as well as antioxidant activities of three cultivars of Pistacia vera cv Kerman were investigated. The total phenolic (TP) content flavonoids (FT) and anthocyanins (TA) were measured. Additionally, the profile of polyphenols was analyzed. A slight, not significant, increment was observed in TP content between cultivars with different age (5, 9 and 11 years old). The 9 years old cultivar showed the highest FL value, while the 11 years old cultivar had the higher TA content. Main polyphenols were separated by HPLC and identified by electrospray ionization (ESI) coupled to quadrupole-time of flight mass spectrometry (LC-ESI-QTOF-MS). Gallic acid and (+)-catechin were present in higher amounts. The presence of myricetin, isoquercitrin and a dimer of procyanidin are reported for the first time in pistachio. Additionally, K, Ca and Mg were found in high proportion. The highest antioxidant capacity was measured in the 11 years old pistachio cultivar. This work presents the first evidence that Pistacia vera cv Kerman from Argentinean cultivars could be considered as a functional food or ingredient in a diet, with potential to improve human health.","palabras_clave":"Pistacia vera cv Kerman|Polyphenolics |Antioxidant activity|Mineral content |Nutritional value"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63878,"idioma_id":4,"titulo":"Lactobacillus rhamnosus CRL1505 enhances systemic and respiratory innate immune response in immunocompromised malnourished mice","resumen":"The effect of Lactobacillus rhamnosus CRL1505 on blood and bone marrow (BM) myeloid cells and their correlation with resistance against Streptococcus pneumoniae infection was evaluated in protein-malnourished mice. Repletion of malnourished mice with supplemental L. rhamnosus improved recovery of BM responsiveness against the infectious challenge through increase in myeloid progenitors and mobilisation of granulocytes. The CRL1505 strain normalised the number of Gr1+ cells and Gr1low\/Gr1high cells balance in BM. These changes in BM correlated with improved neutrophils recruitment, higher phagocytic activity and increased resistance against pneumococcal infection in probiotic-treated mice. This research provides strong evidence of the importance of dietary supplementation with probiotic bacteria to reverse alterations in myeloid progenitors in malnourished mice. The present results strongly suggest that the CRL1505 strain could be used in the development of probiotic foods, which would be especially useful for the recovery of immunocompromised hosts.","palabras_clave":"Lactobacillus rhamnosus CRL1505|myeloid cells|malnutrition|bone marrow| respiratory immunit"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63879,"idioma_id":5,"titulo":"'Los Atlas Históricos de Ciudades: instrumentos para la interpretación de los procesos urbanos. Santa Fe  (Argentina), 1880 - 1945'","resumen":"Este artículo pretende resumir los fundamentos teóricos y los postulados metodológicos que sustentaron la construcción de un Atlas Histórico urbano de una ciudad provinciana del Litoral argentino, Santa Fe. Partiendo de un nutrido y diverso fondo documental, producto de una prolongada tarea de investigación, el atlas fue concebido como un instrumento que permitió sistematizar, representar e interpretar distintas etapas en la conformación de la ciudad durante el transcurso del proceso de modernización (1880-1945). Sobre los documentos cartográficos se operó la lectura crítica y la posterior preparación de una nueva cartografía, restituyendo la secuencia de crecimiento urbano en sucesivos cortes temporales para disponer de una base cartográfica homogénea y producir el mapeo de los distintos indicadores del nivel de urbanización de la ciudad para cada momento histórico.","palabras_clave":"PATRIMONIO DOCUMENTAL|HISTORIA URBANA|REPRESENTACIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63880,"idioma_id":5,"titulo":"Territorialidad y acceso a la tierra. Luchas por legitimar el derecho a la tierra en el Chaco salteño","resumen":"La liberalización de la economía y la desregulación del Estado representaron las condiciones de posibilidad para el avance del modelo del agronegocio en Argentina, vehiculizado principalmente a partir de la expansión del monocultivo de soja transgénica. Ello implicó además un proceso de expansión de la frontera agropecuaria sobre el territorio habitado y en la producción hecha por comunidades indígenas y campesinas, generando aumento de la conflictividad en el agro. El objetivo de este trabajo es analizar y comprender el rol del poder judicial en las disputas jurídicas entre campesinos, indígenas y empresarios en torno a la propiedad de la tierra en el Chaco Salteño. Es en este contexto que nos preguntamos cómo los distintos sujetos intervinientes (campesinos, indígenas, empresarios) representan esta relación y cómo los jueces se trasladan de estos lenguajes (los de las normas) de la imaginación a otro (el de la toma de una decisión, un fallo), formando, por consiguiente, un determinado sentido de la justicia.","palabras_clave":"Liberalización de la economía|Monocultivo de soja transgénica|Comunidades indígenas y campesinas|Disputas legales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63881,"idioma_id":4,"titulo":"Characterization of locomotor activity circadian rhythms in athymic nude mice","resumen":"BackgroundThe relation between circadian dysregulation and cancer incidence and progression has become a topic of major interest over the last decade. Also, circadian timing has gained attention regarding the use of chronopharmacology-based therapeutics. Given its lack of functional T lymphocytes, due to a failure in thymus development, mice carrying the Foxn1(&#916;\/&#916;) mutation (nude mice) have been traditionally used in studies including implantation of xenogeneic tumors. Since the immune system is able to modulate the circadian clock, we investigated if there were alterations in the circadian system of the athymic mutant mice.MethodsGeneral activity circadian rhythms in 2\u20134 month-old Foxn1(&#916;\/&#916;) mice (from Swiss Webster background) and their corresponding wild type (WT) controls was recorded. The response of the circadian system to different manipulations (constant darkness, light pulses and shifts in the light\u2013dark schedule) was analyzed.ResultsFree-running periods of athymic mice and their wild type counterpart were 23.86&#8201;±&#8201;0.03 and 23.88&#8201;±&#8201;0.05 hours, respectively. Both strains showed similar phase delays in response to 10 or 120 minutes light pulses applied in the early subjective night and did not differ in the number of c-Fos-expressing cells in the suprachiasmatic nuclei, after a light pulse at circadian time (CT) 15. Similarly, the two groups showed no significant difference in the time needed for resynchronization after 6-hour delays or advances in the light\u2013dark schedule. The proportion of diurnal activity, phase-angle with the zeitgeber, subjective night duration and other activity patterns were similar between the groups.ConclusionsSince athymic Foxn1(&#916;\/&#916;) mice presented no differences with the WT controls in the response of the circadian system to the experimental manipulations performed in this work, we conclude that they represent a good model in studies that combine xenograft implants with either alteration of the circadian schedules or chronopharmacological approaches to therapeutics.","palabras_clave":"immune|circadian rhythms|nude"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63882,"idioma_id":4,"titulo":"Molecular techniques for Dicistrovirus detection without RNA extraction and purification","resumen":"Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.","palabras_clave":"Triatoma virus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63883,"idioma_id":4,"titulo":"Cryo-electron microscopy reconstructions of Triatoma virus particles: a clue to unravel genome delivery and capsid disassembly","resumen":"Triatoma virus (TrV) is a member of the insect virus family Dicistroviridae and consists of a small, non-enveloped capsid that encloses its positive-sense ssRNA genome. Using cryo-transmission electron microscopy and three-dimensional reconstruction techniques combined with fitting of the available crystallographic models, this study analysed the capsids corresponding to mature and several RNA-empty TrV particles. After genome release, the resulting reconstruction of the empty capsids displayed no prominent conformational changes with respect to the full virion capsid. The results showed that RNA delivery led to empty capsids with an apparent overall intact protein shell  and suggested that, in a subsequent step, empty capsids disassemble into small symmetrical particles. Contrary to what is observed upon genome release in mammalian picornaviruses, the empty TrV capsid maintained a protein shell thickness and size identical to that in full virions.","palabras_clave":"Triatoma virus|Dicistroviridae|cryo-transmission electron microscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63884,"idioma_id":4,"titulo":"Probing the biophysical interplay between a viral genome and its capsid","resumen":"The interaction between a viral capsid and its genome governs crucial steps in the life cycle of a virus, such as assembly  and genome uncoating. Tuning cargo?capsid interactions is also essential for successful design and cargo delivery in engineered viral systems. Here we investigate the interplay between cargo and capsid for the picorna-like Triatoma virus using a combined native mass spectrometry and atomic force microscopy approach. We propose a topology and assembly model in which heterotrimeric pentons that consist of five copies of structural proteins VP1, VP2 and VP3 are the free principal units of assembly. The interpenton contacts are established primarily by VP2. The dual role of the genome is first to stabilize the densely packed virion and, second, on an increase in pH to trigger uncoating by relaxing the stabilizing interactions with the capsid. Uncoating occurs through a labile intermediate state of the virion that reversibly disassembles into pentons with the concomitant release of protein VP4.","palabras_clave":"Triatoma virus|Native mass spectrometry|Atomic force microscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63885,"idioma_id":4,"titulo":"The capacity for paracellular absorption in the insectivorous bat Tadarida brasiliensis","resumen":"Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular processes. The capacity for paracellular absorption seems greater in fliers than in nonfliers, although that conclusion rests mainly on a comparison of flying birds and nonflying mammals because only two frugivorous bat species have been studied. Furthermore, the bats studied so far were relatively large (>85 g, compared with most bat species which are 80 % was accounted for by paracellular absorption. We conclude that passive paracellular absorption of molecules of the size of amino acids and glucose is extensive in this bat and, generally in bats, significantly higher than that in nonflying mammals, although the exact extent can be somewhat lower or higher depending on molecule size, polarity and charge.","palabras_clave":"Intestine|L-arabinose|Mediated absorption|Passive absorption"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63886,"idioma_id":4,"titulo":"Paracellular absorption in laboratory mice: Molecule size-dependent but low capacity","resumen":"Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular processes. The capacity for paracellular absorption seems lower in nonfliers than in fliers, although that conclusion rests largely on a comparison of relatively larger nonflying mammals (> 155 g) and relatively smaller flying birds ( 80% of glucose is absorbed by mediated process(es) rather than the passive, paracellular route. As in all other vertebrates, absorption of cellobiose (f = 0.13 ± 0.02) was even lower than that for l-arabinose, suggesting an equivalent molecular size cut-off for flying and nonflying animals and thus a comparable effective TJ aperture. An important ecological implication is that smaller water-soluble plant secondary metabolites that have been shown to be absorbed by the paracellular path in cell culture, such as phenolics and alkaloids, might be absorbed in substantial amounts by bats and small birds relative to nonflying mammals such as mice.","palabras_clave":"Intestine L-Arabinose |Mediated absorption| Passive absorption |3-O-methyl-D-glucose"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63887,"idioma_id":4,"titulo":"Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis","resumen":"Flying vertebrates have been hypothesized to have a high capacity for paracellular absorption of nutrients. This could be due to high permeability of the intestines to nutrient-sized molecules (i.e., in the size range of amino acids and glucose, MW 75?180 Da). We performed intestinal luminal perfusions of an insectivorous bat, Tadarida brasiliensis. Using radio-labeled molecules, we measured the uptake of two nutrients absorbed by paracellular and transporter-mediated mechanisms (l-proline, MW 115 Da, and d-glucose, MW 180 Da) and two carbohydrates that have no mediated transport (l-arabinose, MW 150 Da, and lactulose, MW 342 Da). Absorption of lactulose (0.61 ± 0.06 nmol min&#8722; 1 cm&#8722; 1) was significantly lower than that of the smaller arabinose (1.09 ± 0.04 nmol min&#8722; 1 cm&#8722; 1). Glucose absorption was significantly lower than that of proline at both nutrient concentrations (10 mM and 75 mM). Using the absorption of arabinose to estimate the portion of proline absorption that is paracellular, we calculated that 25.1 ± 3.0% to 66.2 ± 7.8% of proline absorption is not transporter-mediated (varying proline from 1 mM to 75 mM). These results confirm our predictions that 1) paracellular absorption is molecule size selective, 2) absorption of proline would be greater than glucose absorption in an insectivore, and 3) paracellular absorption represents a large fraction of total nutrient absorption in bats.","palabras_clave":"Arabinose| Bat; Flight|Nutrient absorption|Paracellular absorption; Perfusion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63888,"idioma_id":4,"titulo":"Geoarchaeological and Paleoenvironmental context of the human settlement  in the Eastern Tandilia Range, Argentina","resumen":".","palabras_clave":"ARQUEOLOGIA|PALEOAMBIENTE|GEOARQUEOLOGIA|ZOOARQUEOLOGIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63889,"idioma_id":4,"titulo":"Short-term impact of olive mill wastewater (OMWW) applications on the physico-chemical and microbiological soil properties of an olive grove in Argentina","resumen":"The purpose of this work was to investigate the effects of spreading olive oil mill wastewater (OMWW) on soil biochemical parameters and olive production in an organically managed olive orchard. The experiment was carried out with three different doses of OMWW (80, 160 and 500 m3 ha-1) and a control (untreated soil). Three samplings were done at 10, 30 and 90 days after the administration of the byproduct. OMWW application differentially modified the biochemical properties of the soil analyzed. Organic matter, organic carbon, total nitrogen and extractable phosphorus soil contents increased proportionally with each increasing dose. The values of these parameters decreased gradually with time. Total microbial activity was altered and the OMWW 500 m3 ha-1 treatment proved to be the most active when compared with the other applied doses. OMWW agricultural application also modified the structure of soil microbial communities, particularly affecting Gram positive and negative bacteria, while fungal biomass did not show consistent changes. Although there was a salinity  increase in the treated soil, especially at the highest dose, the productive parameters analyzed (fruit and oil tree-1) were not affected. In light of the obtained results, we consider that low dose of OMWW could be considered an alternative farming practice for semiarid regions.","palabras_clave":"Olea europaea L|olive oil mill wastewater|land spreading|soil microbiological properties|microbial community structure| phospholipids fatty acid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63890,"idioma_id":4,"titulo":"Towards a New Modality-Independent Interface for a Robotic Wheelchair","resumen":"This work presents the development of a robotic wheelchair that can be commanded by users in a supervised way or by a fully automatic unsupervised navigation system. It provides flexibility to choose different modalities to command the wheelchair, in addition to be suitable for people with different levels of disabilities. Users can command the wheelchair based on their eye blinks, eye movements, head movements, by sip-andpuff and through brain signals. The wheelchair can also operate like an auto-guided vehicle, following metallic tapes, or in an autonomous way. The system is provided with an easy to use and flexible graphical user interface onboard a personal digital assistant, which is used to allow users to choose commands to be sent to the robotic wheelchair. Several experiments were carried out with people with disabilities, and the results validate the developed system as an assistive tool for people with distinct levels of disability.","palabras_clave":"Robotic wheelchair|control|interfaces"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63891,"idioma_id":4,"titulo":"Endosymbiotic and host proteases in the digestive tract of the invasive snail Pomacea canaliculata: Diversity, origin and characterization","resumen":"                    Digestive proteases of the digestive tract of the apple snail Pomacea canaliculata were studied. Luminal protease activity was found in the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Several protease bands and their apparent molecular weights were identified in both tissue extracts and luminal contents by gel zymography: (1) a 125 kDa protease in salivary gland extracts and in the crop content; (2) a 30 kDa protease throughout all studied luminal contents and in extracts of the midgut gland and of the endosymbionts isolated from this gland; (3) two proteases of 145 and 198 kDa in the coiled gut content. All these proteases were inhibited by aprotinin, a serine-protease inhibitor, and showed maximum activity between 30ºC and 35ºC and pH between 8.5 and 9.5. Tissue L-alanine-Naminopeptidase activity was determined in the wall of the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Our findings show that protein digestion in P. canaliculata is carried out through a battery of diverse proteases originated from the salivary glands and the endosymbionts lodged in the midgut gland and by proteases of uncertain origin that occur in the coiled gut lumen.                                                            ","palabras_clave":"symbiosis|ampullariids|proteases|digestion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63892,"idioma_id":4,"titulo":"First occurrence of  stomach stones in pterosaurs.","resumen":"Two nearly complete skeletons of the filter-feeding pterodactyloid Pterodaustro guinazui from the Lower Cretaceous of Argentina exhibit clusters of poorly sorted coarse sand to fine gravel inside the abdominal cavity. These stones are interpreted as ingested gastroliths (geogastroliths), which are commonly found in a variety of archosaurs (including birds)but have never before been reported in a pterosaur. The geogastroliths found in these Pterodaustro specimens are interpreted  as having assisted in the digestion of hard food items such as \u2018shelled\u2019 crustaceans that are abundant in the fossil beds of this pterosaur. One of these specimens with geogastroliths has anteriormandibular teeth that are notably thicker than the posterior teeth and are somewhat procumbent. We suggest that these teeth might have facilitated the apprehension of fine gravel.","palabras_clave":"Gastroliths|Pterosaurs|Palaeontology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63893,"idioma_id":5,"titulo":"Apropiación humana de la producción primaria en el Chaco Seco","resumen":"      El  Chaco Seco experimenta altas tasas de deforestación y transformación  productiva. Estimamos la magnitud de la apropiación humana de la productividad  primaria neta (PPN) aérea de esta región por vías directas (producción  ganadera, forestal y agrícola) e indirectas (fuego), su distribución regional,  su relación con variables climáticas y socioeconómicas y sus contrastes entre  vegetación natural, pasturas y cultivos. Para 69 departamentos se utilizaron  datos censales, climáticos y satelitales (período 2002-2006) a partir de los  que se estimó la PPN aérea con modelos climáticos, la apropiación de forraje  por ganadería con datos de stock animal y modelos metabólicos, la apropiación  de productos agrícolas y forestales con estadísticas nacionales y apropiación indirecta  de biomasa por fuego a partir sensores remotos. La ganadería, desarrollada bajo  vegetación natural y pasturas (90.9% del área), involucró una apropiación de  248 kgMS.ha-1.año-1,  y aumentó en departamentos con mayor fracción de pasturas, ganado agrupado en  rodeos más grandes y menores niveles de pobreza. La apropiación forestal,  desarrollada bajo vegetación natural (88.7%), alcanzó 64 kgMS.ha-1.año-1  representados redominantemente por carbón vegetal y leña, y fue  mayor en departamentos de clima más húmedo. En la fracción agrícola del  territorio (6.3%), la apropiación directa trepó a 2293 kgMS.ha-1.año-1,  representada principalmente por grano de soja y maíz. La apropiación indirecta  por fuego promedió 746 kgMS.ha-1.año-1  para todo el territorio. Este flujo estuvo positivamente  asociado a la tasa de desmonte, ascendiendo de ~450 a &gt;2000 kgMS.ha-1.año-1  al pasar de departamentos con mínimo a máximo desmonte. En la  actualidad, la baja apropiación de la PPN de la vegetación natural del Chaco  Seco constituye un incentivo para su reemplazo por pasturas y cultivos anuales.  Una actividad ganadera y\/o forestal más eficiente en los bosques representa un desafío  tecnológico y político prioritario capaz de reconciliar la producción y la  conservación en esta región.  ","palabras_clave":"BOSQUE|DESMONTE|FUEGO|GANADERÍA|PASTURAS|PRODUCTIVIDAD PRIMARIA NETA|USO FORESTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63894,"idioma_id":4,"titulo":"Effect of experimental diabetic retinopathy on the non-image forming visual system","resumen":"Chronobiol Int. 2013 May;30(4):583-97. doi: 10.3109\/07420528.2012.754453. Epub  2013 Feb 27.  Effect of experimental diabetic retinopathy on the non-image-forming visual system.  Fernandez DC, Sande PH, de Zavalía N, Belforte N, Dorfman D, Casiraghi LP, Golombek D, Rosenstein RE.    Source  Laboratorio   de Neuroquímica Retiniana y Oftalmología Experimental, Departamento de   Bioquímica Humana, Facultad de Medicina\/CEFyBO, Universidad de Buenos   Aires\/CONICET, Buenos Aires, Argentina.    Abstract    Diabetic   retinopathy is a leading cause of blindness. Intrinsically   photosensitive retinal ganglion cells (ipRGCs), which express the   photopigment melanopsin, are involved in non-image-forming visual   responses such as photoentrainment of circadian rhythms and pupillary   light reflex. Since several reports indicate that retinal ganglion cells   are affected by diabetes, we investigated the non-image-forming visual   system in an advanced stage of experimental diabetes in rats induced by   streptozotocin. After 15 wks of diabetes induction, clear alterations in   the visual function were observed and all animals developed mature   cataracts. At this time point, concomitantly with a significant decrease   in the number of Brn3a(+) retinal ganglion cells, no differences in the   number of melanopsin-containing cells, melanopsin levels, and retinal   projections to the suprachiasmatic nuclei and the olivary pretectal   nucleus were observed. At high light intensity, afferent pupil light   reflex appears to be conserved in diabetic animals. After 15 wks of   diabetes induction, a significant decrease in light-induced c-Fos   expression in the suprachiasmatic nuclei was found. In diabetic animals,   the locomotor activity pattern was conserved, although a delay in the   time needed for re-entrainment after a phase delay was observed. In   diabetic animals, lensectomy reversed the alterations in c-Fos   expression and in the locomotor activity rhythm. These results suggest   that the neuronal substrate of the non-image-forming visual system   remained largely unaffected at advanced stages of diabetes, and that   lensectomy, a relatively easy and safe surgery, could partially restore   circadian alterations induced by diabetes.","palabras_clave":"experimental diabetic retinopathy|retinal ganglion cells|melanopsin|pupil light reflex"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63895,"idioma_id":4,"titulo":"- OVARIECTOMY CAUSES OVEREXPRESSION OF RENAL NA+,K+ -ATPASE      AND SODIUM SENSITIVE HYPERTENSION IN ADULT WISTAR RATS","resumen":"We investigated the effect of ovariectomy(oVx) on renal and systemic hemodynamic, electrolyte excretion and total and dephosphorylated Na(+),K(+)-ATPase &#945;1 subunit (t-d-NKA) in normotensive Wistar rats under a normal sodium (NS, 0.24%) or high sodium (HS, 1%) intake versus intact female (IF). On NS intake, t-d-NKA was higher in oVx rats and overexpressed in the thick ascending limbs (P < .01 vs. IF) and renal plasma flow was increased. On HS intake, oVx rats maintained a greater dephosphorylated NKA, excreted less sodium, and developed arterial hypertension (134 ± 4 vs. IF 112 ± 5 mm Hg, P < .05). Sodium load caused salt-sensitive hypertension in oVx Wistar rats.","palabras_clave":"ovariectomy|renal Na+, K+-ATPase|phospho state|sodium sensitive hypertension|mTAL|female hormones|sodium excretion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63896,"idioma_id":4,"titulo":"Water relations, biochemical \u2013 physiological and yield responses ofolive trees (Olea europaea L. cvs. Arbequina and Manzanilla) underdrought stress during the pre-flowering and flowering period","resumen":"In arid and semiarid regions from Argentina, where the main olive production areas are located, evapo-transpiration is high and rainfall is minimal during winter and spring months, as compared with theMediterranean region where winter rainfall precludes the need of irrigation in such period. The aim ofthe work was to study water relations, biochemical\u2013physiological and yield responses of olive trees (Oleaeuropaea L., Arbequina and Manzanilla cultivars) under different drought stress levels applied during thepre-flowering\u2013flowering period. Increasing levels of water deficit affected plant water relations as mea-sured by pronounced drops of stem water potentials (near &#8722;4.0 MPa) in treatments with severe waterdeprivation at the end of the flowering period. Deficit irrigation was associated with some leaf-levelbiochemical-physiological responses (accumulation of osmotically active substances, increased concen-tration of high molecular weight hydrocarbons and cuticle thickening), which can be interpreted asadaptation mechanisms of olive to water deficit. Water stress was also associated with increased lipidperoxidation and decreased levels of photosynthetic pigments, stomatal conductance and photosyntheticrate. During the first crop year analyzed, a significant decrease in fruit set and fruit yield was observedin treatments under water deprivation. Also, all treatments evaluated showed strong drops in fruitingand yield parameters during the second crop year suggesting a marked bearing pattern for both olivecultivars. From a practical standpoint, little irrigation (50% ETc) may be sufficient to maintain adequateplant water potentials for the coldest winter months, but high (75% ETc) or full (100% ETc) irrigation ratescould be needed by mid-August (approximately 2 months before flowering) to avoid detrimental effectsof water stress on biochemical\u2013physiological and yield parameters of olive trees cultivated in areas withdry winter-spring season.","palabras_clave":"Leaf-level responses|Olive|Pre-flowering\u2013flowering period|Water stress|Yield responsesa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63897,"idioma_id":4,"titulo":"An Enrichment Scheme for Solidification Problems","resumen":"A new enriched finite element formulation for solving isothermal phase change problems is presented. We propose a fixed mesh method, where the discontinuity in the temperature gradient is represented by enriching the finite element space through a function whose definition includes a gradient discontinuity. Generally, in these types of formulations, the enrichment location (the location of the solidication front) is determined through a level set auxiliary scheme. In this work, this position is determined implicitly by constraining the temperature at the phase change boundary to be equal to the melting temperature. Several numerical examples are presented to show the application of the method.","palabras_clave":"Enriched Finite Element Method|Solidi\fcation Problems|Stefan Problem|Phase Change Problems"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63898,"idioma_id":4,"titulo":"Degradation of cationic surfactants using Pseudomonas putida A ATCC 12633 immobilized in calcium alginate beads","resumen":"In this study, the degradation of tetradecyltrimethylammonium bromide (TTAB) by freely suspended and alginate-entrapped cells from the bacteria P. putida A ATCC 12633 was investigated in batch cultures. The optimal conditions to prepare beads for achieving a higher TTAB degradation rate were investigated by changing the concentration of sodium alginate, pH, temperature, agitation rate and initial concentration of TTAB. The results show that the optimal embedding conditions of calcium alginate beads are 4% w\/v of sodium alginate content and 2 x 108 cfu ml-1 of P. putida A ATCC cells that had been previously grown in rich medium. The optimal degradation process was carried out in pH 7.4 buffered medium at 30 ºC on a rotary shaker at 100 rpm. After 48 h of incubation, the free cells degraded 26 mg l-1 of TTAB from an initial concentration of 50 mg l-1 TTAB. When the initial TTAB concentration was increased to 100 mg l-1, the free cells lost their degrading activity and were no longer viable. In contrast, when the cells were immobilized on alginate, they degraded 75% of the TTAB after 24 h of incubation from an initial concentration of 330 mg l-1 of TTAB. The immobilized cells can be stored at 4 °C for 25 days without loss of viability and can be reused without losing degrading capacity for 3 cycles.                               ","palabras_clave":"Biodegradation.|Tetradecyltrimethylammonium|Pseudomonas putida|Alginate"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63899,"idioma_id":5,"titulo":"Políticas, ideas y expertos en la cuestión social de la Argentina democrática (1983-2012)","resumen":"Historia de los principales debates sobre orientación de la política social desde 1983 a la actualidad.","palabras_clave":"POLITICA SOCIAL|HISTORIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63900,"idioma_id":4,"titulo":"Optical coherence tomography-based scanning  system for shape determination, wall thickness mapping, and inner  inspection of glass containers","resumen":"In this work we present a method that enables simultaneous measurement of shape and wall parameters of glass containers. The system is based on the optical coherence tomography technique, employing the spectral domain configuration. The data were obtained by measuring spatial coordinates of a sequence of points in a predefined region of a sample that includes points on the surface and in the interior of the material.  Dimensional parameters, thickness mapping, and tomography studies of the interior of the sample walls, can be obtained from these measurements","palabras_clave":"Interferometría óptica|Ensayo no destructivo|Medición de espesores en vidrios"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63901,"idioma_id":4,"titulo":"Polypropylene\/clay nanocomposites produced by SCORIM: deformation and fracture properties","resumen":"The effect of distinct morphologies induced by shear controlled orientation in injection molding (SCORIM) in the mechanical and fracture performance of polypropylene (PP) and PP\/nanoclay mouldings is examined in this work. The effect of high shear conditions applied during processing was assessed. Samples exhibited a range of fracture stability ranging from a modest non-linearity to a quasi-stable regime depending on material type and injection condition. Neat PP showed non-linear brittle behaviour while nanocomposites exhibited quasi-stable behaviour induced by the large deformation capability of the skin layer. Despite the fracture initiating at practically the same loading levels, the propagation energy varied with processing conditions and nanoclay content. The reduction of the core layer achieved by the SCORIM processing along with the differences between skin and core favoured by the presence of nanoclay are responsible of the toughening of SCORIM PP\/nanoclay thick mouldings","palabras_clave":"NANOCOMPOSITES|POLYPROPYLENE|SCORIM|FRACTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63902,"idioma_id":4,"titulo":"INTERSPECIFIC CROSSES BETWEEN JACARANDA MIMOSIFOLIA AND J. CUSPIDIFOLIA (BIGNONIACEAE)","resumen":"Jacaranda mimosifolia, which has beautiful, long-lasting blue flowers, is a subtropical tree native to northern Argentina. It has been widely planted, not only in subtropical, but also in temperate areas where there is no risk of frost. We are conducting a breeding program for jacaranda aiming to select for dwarf and juvenile flowering characteristics that are suitable for potted plants. J. cuspidifolia, which has reddish-purple flowers and larger leaves than J. mimosifolia and is also distributed in northern Argentina, has juvenile flowering characteristics. We attempted interspecific crossing between J. mimosifolia and J. cuspidifolia. A relatively high frequency of fruit set was observed in crosses of J. mimosifolia × J. cuspidifolia, whereas very little fruit set was observed in the reciprocal cross (J. cuspidifolia × J. mimosifolia). Based on microscopic observations, pollen tubes penetrated the style and reached the ovary in crosses of J. mimosifolia × J. cuspidifolia. On the other hand, few pollen tubes reached the ovary in J. cuspidifolia × J. mimosifolia crosses. The germination rate of seeds obtained in crosses between J. mimosifolia × J. cuspidifolia was relatively high, whereas it was quite low in the seeds obtained from the reciprocal cross. All of the seedlings obtained from these interspecific crosses were confirmed as hybrids by isozyme analysis. Several characteristics of these hybrids and their parents were also investigated. Citation ","palabras_clave":"Jacaranda mimosifolia|interspecific crossing|Bignoniaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63903,"idioma_id":4,"titulo":"Hsp90-Binding Immunophilins as a Potential New Platform for Drug Treatment","resumen":"Immunophilins are proteins that contain a peptidyl-prolyl-(cis\/trans)-isomerase domainas a family signature. Low molecular weight immunophilins were first described associated to immunosuppressive action and protein folding. Recent studies of other members of the family have led to the identification of their participation in basic processes such as protein-protein interactions, signal transduction cascades, cell differentiation, cell cycle progression, metabolic activity, apoptosis mechanisms, microorganisms infection, cancer, neurotrophism and neuroprotection, among several other physiological and pathophysiological processes. Due to all these emerging features, the developing of specific ligands for immunophilins appears to have promising perspectives during the following years, in particular in the cancer biology and neuroregeneration fields. In this article we discuss the emerging role of immunophilins in protein transport, transcription regulation, malignancies development, and neurotrophic action, a number of biologicalproperties that transforms these proteins in potential targets for novel therapeutic strategies. peptidyl-prolyl-(cis\/trans)-isomerase domainas a family signature. Low molecular weight immunophilins were first described associated to immunosuppressive action and protein folding. Recent studies of other members of the family have led to the identification of their participation in basic processes such as protein-protein interactions, signal transduction cascades, cell differentiation, cell cycle progression, metabolic activity, apoptosis mechanisms, microorganisms infection, cancer, neurotrophism and neuroprotection, among several other physiological and pathophysiological processes. Due to all these emerging features, the developing of specific ligands for immunophilins appears to have promising perspectives during the following years, in particular in the cancer biology and neuroregeneration fields. In this article we discuss the emerging role of immunophilins in protein transport, transcription regulation, malignancies development, and neurotrophic action, a number of biologicalproperties that transforms these proteins in potential targets for novel therapeutic strategies. ","palabras_clave":"Chaperones|TPR protein|peptidyl-prolyl isomerase|heat-shock proteins|neurodifferentiation|shuttling|tacrolimus|FK506"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63904,"idioma_id":4,"titulo":"Diet of Hyphessobrycon auca (Pisces, Characidae) in Iberá wetland (Northeastern, Argentina)","resumen":"Hyphessobrycon auca...","palabras_clave":"DIET|OMNIVORY|HERVIBORIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63905,"idioma_id":4,"titulo":"ARTERIAL STIFFNESS AND ENDOTHELIAL FUNCTION IN OBESE CHILDREN AND ADOLESCENTS AND ITS RELATIONSHIP WITH CARDIOVASCULAR RISK FACTORS","resumen":"Obesity is related to an increase in the rates of cardiovascular disease. Objective: To establish the impact of obesity on vascular function (endothelial function and arterial stiffness) in children and adolescents and its relation to cardiovascular risk factors.   Methods: In obese children and adolescents, endothelial function and arterial stiffness were evaluated by pulse plesthysmography method (reactive hyperaemia and index of digital volume waveforms, respectively). Data were compared with non-obese group (Body Mass Index (BMI): >10th - <97th percentile). In both groups anthropometric parameters, body fat percentage, fasting glucose, lipid profile, insulinemia, HOMA-IR and hemodynamic parameters were determined.    Results: BMI, weight, waist circumference, body fat, insulinemia and HOMA-IR were significantly higher in obese. Obese showed impaired endothelial function (15.8±.2%; n=37) than non-obese (41.4±5%; n=20; p<0.001) and increased arterial stiffness. Only in obese, endothelial function was negative correlated with waist circumference and HOMA-IR, whereas a positive correlation was found between insulinemia and HOMA-IR.  Conclusions: This study shows that an impaired vascular function is already present in obese children and adolescent. The fact that the obesity is associated with some markers of cardiovascular risk suggests the importance of make early interventions in lifestyle of this population to prevent the occurrence of cardiovascular disease.","palabras_clave":"obesidad|emdotelio |rigidez arterial"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63906,"idioma_id":4,"titulo":"Reactivity of iron (II) - bound nitrosyl hydride (HNO, nitroxyl) in aqueous solution","resumen":"The reactivity of coordinated nitroxyl (HNO) has been explored with the [FeII(CN)5HNO]3&#8722; complex in aqueous medium, pH 6. We discuss essential biorelevant issues as the thermal and photochemical decompositions, the reactivity toward HNO dissociation, the electrochemical behavior, and the reactionswith oxidizing and reducing agents. The spontaneous decomposition in the absence of light yielded a two-electron oxidized species, the nitroprusside anion, [FeII(CN)5NO]2-, and a negligible quantity of N2O, with kobs&#8776;5×10&#8722;7 s&#8722;1, at 25.0 °C. Thevalue of kobs represents an upper limit for HNO release, comparable to values reported for other structurally related L ligands in the [FeII(CN)5L]n- series. These results reveal that the FeN bond is strong, suggesting a significant &#963;?&#960; interaction, as already postulated for other HNO-complexes. The [FeII(CN)5HNO]3- ion showed a quasi-reversible oxidation wave at 0.32 V (vs normal hydrogen electrode), corresponding to the [FeII(CN)5HNO]3-\/[FeII(CN)5NO]3-,H+ redox couple. Hexacyanoferrate(III), methylviologen and the nitroprusside ion have been selected as potential oxidants. Only the first reactant achieved a completeoxidation process, initiated by a proton-coupled electron transfer reaction at the HNO ligand, withnitroprusside as a final oxidation product. Dithionite acted as a reductant of [FeII(CN)5HNO]3-, in a 4-electron process, giving NH3. The high stability of bound HNO may resemble the properties in related Fe(II) centers of redox active enzymes. The very minor release of N2O shows that the redox conversions may evolve without disruption of the FeN bonds, under competitive conditions with the dissociation of HNO.","palabras_clave":"NITROXYL|NITROSYL HYDRIDE|PENTACYANOFERRATE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63907,"idioma_id":4,"titulo":"Study of magnetic state of Sn0.9Fe0.1O2 powders at low temperature","resumen":"We report on magnetic state at low temperatures of oxide diluted magnetic semiconductor powders (Sn0.9Fe0.1O2) prepared by ball milling technique as a function of milling time.  After 2h of mechanical work, the crystallites of SnO2 reach its minimum size (nearly 10 nm) and do not evolve with increasing duration of milling. The magnetic state of the dopant of ground samples by 2, 5 and 10 hours is mainly magnetically ordered at low temperatures in contrast to the mainly paramagnetic behavior at room temperature. The change of the state becomes evident from the magnetization curves of zero field cooling - field cooling, reinforced by the Mössbauer measurements at 4.2 K and AC-susceptibility (16 - 325 K). Combining these results with measurements of magnetization versus applied magnetic field at 5-10 K, it can be concluded that in 5h milled sample all the Fe incorporated into the rutile structure shows superparamagnetic behavior at RT and they are magnetically ordered at low temperature. Milling times less or greater than 5 hours lead to the occurrence of spurious phases.","palabras_clave":"SnO2 rutile|Low temperature magnetic measurements|Mössbauer spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63908,"idioma_id":4,"titulo":"Electric field gradients of CeMIn5 (M = Co, Rh, Ir) heavy-fermion systems studied by perturbed angular correlations and ab initio electronic structure calculations","resumen":"The electric field  gradient (EFG) at the highly dilute nuclear probe 111Cd in the heavy fermion  systems CeMIn5, M = Co, Rh, and Ir and YCoIn5 has been  investigated by perturbed angular correlation (PAC) measurements of the nuclear  electric quadrupole interaction (QI) of 111Cd on In sites. Pure and Sn-doped  single crystals prepared by In-flux synthesis and polycrystalline samples  prepared by arc melting have been studied. The samples were doped with the PAC  probe 111Cd by diffusion of the mother isotope 111In. In all samples, several  fractions of 111Cd probe nuclei subject to different QIs have been observed,  among them a large fraction of 111Cd in unreacted In metal. Detailed  calculations of the EFG at In nuclei and at Cd probes on In sites of pure and  Sn-doped CeMIn5 were preformed, using the full-potential augmented plane  wave+local orbital (APW+lo) formalism and taking into account different  variables such as the electronic structure of the hyperfine probes, probe  induced structural distortions, and impurity doping. The excellent agreement  between the predicted EFG?s and the experimental results allows us to  assign two of the observed EFG components to the lattice sites 1c and 4i  of the CeMIn5 compounds and to explain the pronounced difference of  the EFG at In and Cd probes on the same lattice position. Structural  distortions induced by the Cd probe and Sn-doping were found to have little  effect on the EFG at the Cd probes. We also show that the local spin density  approximation (LSDA) and LDA+U calculations predict very similar  equilibrium structures and EFG?s at the In\/Cd sites. The extension of the  experiments and the calculations from CeMIn5 to YCoIn5 and LaCoIn5 have  established that the influence of the 4f electrons on the EFG?s at impurity  sites is negligibly","palabras_clave":"Ab-initio|Heavy Fermions|Electric Field Gradient"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63909,"idioma_id":4,"titulo":"Production of chimeric embryos by aggregation of bovine egfp eight-cell stage blastomeres with two-cell fused and asynchronic embryos","resumen":"Embryo disaggregation allows the production of two to four identical offspring from a single cow embryo. In addition, embryo complementation has become the technique of choice to demonstrate the totipotency of embryonic stem cells and induced pluripotent stem cells. Therefore, the aim of this study was to generate a new and simple method by aggregation in the well-of-the-well system to direct each single enhanced green fluorescent protein (egfp) eight-cell blastomere derived from bovine in vitro fertilization embryos to the inner cell mass (ICM) of chimeras produced with fused and asynchronic embryos. To this end, the best conditions to generate in vitro fertilization-fused embryos were determined. Then, the fused (F) and nonfused (NF) embryos were aggregated in two distinct conditions: synchronically (S), with both transgenic and F embryos produced on the same day, and asynchronically (AS), with transgenic embryos produced one day before F embryos. The highest fusion and blastocysts rates were obtained with two pulses of 40 V. The 2ASF and 2ASNF groups showed the best number of blastocysts expressing the EGFP protein (48% and 41%, respectively). Furthermore, the 2ASF group induced the highest localization rates of the egfp-expressing blastomere in the ICM (6\/13, 46% of ICM transgene-expressing blastocysts). This technique will have great application for multiplication of embryos of high genetic value or transgenic embryos and also with the generation of truly bovine embryonic stem cells and induced pluripotent stem cells.","palabras_clave":"Aggregation|Bovine|Chimera|Transgene"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63910,"idioma_id":4,"titulo":"Sequence analysis of the internal transcribed spacer 2 (ITS2) from Philornis seguyi (García, 1952) and Philornis torquans (Nielsen, 1913) (Diptera: Muscidae)","resumen":"Philornis Meinert, 1890 (Diptera: Muscidae) is a genus of Neotropical dipterans that parasitise birds. The currently used external morphological characters to distinguish between species within this genus present some limitations. We used the second internal transcribed spacer region (ITS2) of the rRNA gene as a molecular marker to differentiate adult specimens of Philornis identified morphologically as P. torquans and P. seguyi from different localities. Specimens identified as P.seguyi from Magdalena (Buenos Aires Province) showed an ITS2 sequence different from that for P. torquans, whereas all other specimens of P. seguyi had sequences identical to those for P. torquans. These findings do not necessarily confirm that specimens from Magdalena indeed belong to P. seguyi, nor that P. seguyi is a valid species. Instead, they alert us  about the potential for species misidentification when using morphological characters alone. The use of molecular approaches to aid the identification of Philornis spp. will shed light on the systematics of this group. Philornis torquans is reported for the first time in Mendoza Province and Uruguay.","palabras_clave":"AVIAN|ECTOPARASITE|ITS2|PHILORNIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63911,"idioma_id":4,"titulo":"Controlling the interaction between cells and silica nanoparticles","resumen":"In recent years, the application of silica nanoparticles in the biomedical field  experienced a great development, showing a sharp increase in the number of published  articles and patents. The driving forces for these and future developments are the  possibility to design nanoparticles with homogeneous size and structure and amenable  to specific grafting. In this way, it is possible to control the interaction of nanoparticles  with cells. Moreover, it is possible to tune the characteristics of the nanoparticles to  meet the requirements of each specific cell and desired application. Herein, we present  different strategies developed to optimize the size, morphology, surface topography,  elemental ratio, hydrophobic\/hydrophilic balance, and erosion rate, which contribute to  understand the nature of this inherently complicated cell-nanoparticles interactions  mechanism, which will determine the resulting function performance.","palabras_clave":"CELLS|NANOPARTICLES|INTERACTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63912,"idioma_id":4,"titulo":"Lerner's inequality associated to a critical radius function and applications","resumen":"This work deals with weighted inequalities of the type&#8747;Rd|Tf(x)|pw(x)dx&#8804;C&#8747;Rd|Sf(x)|pw(x)dx, Turn MathJax offwhere  S  is some maximal operator and  T  is an operator that comes from the harmonic analysis associated to a critical radius function. The weight  w  belongs to an appropriate family and  0<p<&#8734; . The proofs are based on an adapted Lerner?s inequality and some point-wise estimates. The results can be applied to obtain inequalities for several operators associated to the Schrödinger semigroup.","palabras_clave":"LERNER INEQUALITY|CRITICAL RADIUS|SCHROEDINGER|COMPARAISON INEQUALITIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63913,"idioma_id":4,"titulo":"NMR-SSC Magnetic Field Profiler Applied to Magnetic Field Shimming","resumen":"A new methodology aimed to control the spatial dependence of a magnetic field within a volume of interest is described. The designed hardware uses Nuclear Magnetic Resonance (NMR) to sense the magnetic field. A modified version of the Statistical Signal Characterization (SSC) method was used to analyze the NMR signal, providing a dynamical definition of the characterization parameters. The information is used to control the currents driving a set of coils that introduce corrections in the spatial dependence of the main field generated by an independent unit (magnet or electromagnet). As a practical matter, we deal with the particular case of magnetic field shimming.","palabras_clave":"MAGNETIC FIELD|SHIMMING|HOMOGENEITY|NUCLEAR MAGNETIC RESONANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63914,"idioma_id":4,"titulo":"Agarofuran Sesquiterpenes from Schaefferia argentinensis as Potential Antiproliferative Agents","resumen":"Sixteen dihydro-&#946;-agarofuran sesquiterpenes were isolated from the aerial parts of Schaefferia argentinensis. The in vitro antiproliferative activity of the major natural compounds were examined against T47D, MCF7, and MDA human solid tumor cancer cell lines.","palabras_clave":"SCHAEFFERIA ARGENTINENSIS|CELASTRACEAE|PHYTOCHEMISTRY|ANTIPROLIFERATIVE ACTIVITY|DIHYDRO-&#914;-AGAROFURAN SESQUITERPENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63915,"idioma_id":5,"titulo":"El desarrollo de estrategias de aprendizaje durante las trayectorias estudiantiles universitarias. Comparación de su logro en el tramo inicial y final de la carrera","resumen":"Se da por sentado que los estudiantes universitarios poseen al ingresar al nivel superior estrategias de aprendizaje que le permitirán aprender y estudiar en la universidad. Pero, ¿con  qué estrategias cuentan desde el inicio de la carrera? ¿qué estrategias de aprendizaje se desarrollan a lo largo de la carrera?¿Hay variaciones entre alumnos que se perciben con dificultades y quienes no? Se intentará responder a estas preguntas en el desarrollo de los resultados que se exponen que forman parte de una línea de investigación marco sobre el aprendizaje en Educación Superior. La hipótesis es: los alumnos que se encuentran en el tramo final de la carrera se diferencian de los alumnos del tramo inicial respecto de las estrategias que emplean para aprender. La población son estudiantes universitarios del tramo inicial (primeros años) y del tramo final (cuarto año) de las Facultades de Filosofía y Letras, Medicina y Ciencias Políticas de la UNCuyo- Argentina y de la Facultad de Educación de la Universidad Federal de Río de Janeiro (UFRJ)- Brasil. El relevamiento de la información se hizo a través de un cuestionario semi-estructurado validado para estudios anteriores. Los resultados brindan evidencia sobre avances en el logro de la autorregulación en algunas estrategias de aprendizaje por parte de estudiantes universitarios y permiten identificar perfiles de alumnos universitarios que aprenden con o sin dificultad en orden a proyectar orientaciones a docentes y asesores universitarios.","palabras_clave":"APRENDER|UNIVERSIDAD|ESTRATEGIAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63916,"idioma_id":4,"titulo":"Preparation and antitrypanosomal activity of secochiliolide acid derivatives","resumen":"Secochiliolide acid (1) isolated from the Patagonian shrub Nardophyllum bryoides, was used  as a scaffold for the preparation of a series of nine derivatives. Compound 1 and its derivatives were  tested against Trypanosoma cruzi epimastigotes grown in liquid media. It was first observed that  secochiliolide acid (1) inhibited the proliferation of the parasites, with an IC50 of 2 µg\/ml. Six of the  synthesized derivatives were also active with IC50s between 2 and 7 µg\/ml which are comparable to  that of the commercial drug benznidazole (2.5 µg\/ml). These results indicate that the carboxyl group is  not essential for the bioactivity of 1, while the presence of the tetrasubstituted exocyclic double bond  seems to be important. Moreover, the presence of the furan and spirolactone rings is not essential for  the bioactivity per se, but is important in combination with other structural fragments present in the  molecule.","palabras_clave":"SECOCHILIOLIDE ACID|TRYPANOSOMA CRUZI|NARDOPHYLLUM BRYOIDES|TRYPANOCIDAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63917,"idioma_id":4,"titulo":"Effects of temperature and salinity on the ovarian cycle and the embryonic development of the invasive shrimp Palaemon macrodactylus","resumen":"The oriental shrimp Palaemon macrodactylus Rathbun, 1902 was detected first in Argentina in 2000 in a fully marine environment (Mar del Plata) and more recently, in two estuarine areas. Here we examined combined effects of four salinities (1, 5, 12, 23, 34 psu) and two temperatures (20°, 25°C) on survival, reproductive cycle of females and embryonic development in shrimps from the marine population of Argentina. Survival was higher than 80% in all the treatments; however, at the lowest salinity (1 psu), all females died after 12 days irrespectively of the temperature condition. Most shrimps (&gt;80%) developed their ovaries and produced eggs at all salinity?temperature tested combinations, but all females lost their eggs after 2 days at 5 psu; a complete embryonic development occurred only at 12 and 34 psu. At the lowest temperature, the ovarian development was delayed in different proportions for individual levels of salinity. Therefore, P. macrodactylus from Mar del Plata harbor survived and completed all reproductive events at salinities 5 psu, but embryonic development was only successful at salinities  12 psu. Our results indicate that this non-native species is capable of invading estuarine habitats over the Argentinean coast but not oligohaline or freshwater environments.","palabras_clave":"brackish water|invasive species|Palaemon macrodactylus|reproduction|salinity and temperature tolerance"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63918,"idioma_id":4,"titulo":"Influence of overlapping genes on the evolution of human hepatitis B virus","resumen":"The aim of this work was to analyse the in&#64258;uence of overlapping genes on the evolution of hepatitis B virus (HBV). A differential evolutionary behaviour among genetic regions and clinical status was found. Dissimilar levels of conservation of the different protein regions could derive from alternative mechanisms to maintain functionality. We propose that, in overlapping regions, selective constraints on one of the genes could drive the substitution process. This would allow protein conservation in one gene by synonymous substitutions while mechanisms of tolerance to the change operate in the overlapping gene (e.g. usage of amino acids with high-degeneracy codons, differential codon usage and replacement by physicochemically similar amino acids). In addition, differential selection pressure according to the HBeAg status was found in all genes, suggesting that the immune response could be one of the factors that would constrain viral replication by interacting with different HBV proteins during the HBeAg(&#8722;) stage.","palabras_clave":"Hepatitis B Virus|Evolution|Overlapping genes|Selective pressure|Codon usage bias"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63919,"idioma_id":4,"titulo":"Granulocyte colony-stimulating factor partially repairs the damage provoked by Trypanosoma cruzi in murine myocardium","resumen":"Background: The hallmark of Trypanosoma cruzi infection is cardiomyopathy that leads to end-stage heart failure.We investigated whether G-CSF, known to induce heart tissue repair by bone marrow stem cell mobilization,ameliorates T. cruzi-induced myocarditis.Methods and results: T. cruzi-infected C3H\/He mice were treated with G-CSF and monitored for parasite burden,BMSC mobilization, cytokine profile and cardiac remodeling.G-CSF increased the expression of CXCR4, CD34, and c-Kit, indicatingmobilization and migration of BMSC, some ofwhich differentiated to cardiomyocytes as evidenced by increased levels of GATA4+\/MEF2C+ cells and desmin expressionin chagasic hearts. G-CSF enhanced amixed cytokine response (IL-10 + TGF-&#946; > IFN-&#947; + TNF-&#945; > IL-4)associated with increased heart inflammation and no beneficial effect on parasite control. Further, G-CSF controlledT. cruzi-induced extracellular-matrix alterations of collagens (Col I and Col llI), hydroxyproline, and glycosaminoglycancontents and promoted compensatory cardiac remodeling; however, these responses were not sufficientto control T. cruzi-induced cardiomyocyte atrophy. Benznidazole treatment prior to G-CSF resulted in the controlof parasitism and parasite-induced inflammation, and subsequently, G-CSF was effective in executing the tissue repair,as evidenced by extracellular-matrix homeostasis and normalization of cardiomyocyte size in chagasic hearts.Conclusions: G-CSF treatment after T. cruzi infection enhanced migration and homing of BMSC, some of which differentiatedto cardiomyocytes. Yet, G-CSF promoted a mixed (Treg > Th1 > Th2) immune response that contributedto persistent inflammation and limited improvement in cardiac homeostasis. Combinatorial therapy(BZ &#8594; G-CSF) was beneficial in arresting inflammatory processes and tissue damage in chagasic hearts.","palabras_clave":"Chagas disease|Trypanosoma cruzi|G-CSF|Stem cell"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63920,"idioma_id":4,"titulo":"Magnetic Properties of Zn-Ferrites Obtained From Multilayer Film Deposited by Sputtering","resumen":"Epitaxial ZnFe O thin films were deposited onmonocrystallineMgO substrate by dc-sputtering. Ferrites were grown from Zn-O and Fe-O multilayers starting from metallic targets in oxygen atmosphere. The number and thickness of layers were varied holding constant the total thickness (50 nm) and sample stoichiometry (Fe\/Zn=2) . The samples were structural and magnetically characterized by X-ray diffraction and magnetic measurements, respectively. Thin film fabricated using 20 bilayers of thickness 3 nm present a magnetic behavior similar to bulk ZnFe2O4, with a Néel temperature TN=10.5 K. The magnetic behavior of those films obtained from thicker bilayers (6 and 10 nm) is characterized by the coexistence of antiferro and ferromagnetic regions. This is probably due to the inhomogeneities in Zn and Fe distributions caused by an incomplete interdiffusion process prevented by the relatively large multilayer thickness. These inhomogeneities probably generate zinc-iron position replacements and interrupt the long-range antiferromagnetic order, giving  rise to ferromagnetic clusters coexisting with the antiferromagnetic component.","palabras_clave":"DILUTED ANTIFERROMAGNETISM (DAFF) SYSTEM|DC- SPUTTERING|FERRITE FILM|MAGNETIC ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63921,"idioma_id":4,"titulo":"Isomorphism of graph classes related to the circular-ones property","resumen":"We give a linear-time algorithm that checks for isomorphism between two 0- 1 matrices that obey the circular ones property. Our algorithm is similar to the isomorphism algorithm for interval graphs of Lueker and Booth, but works on PC trees, which are unrooted and have a cyclic nature, rather than with PQ trees, which are rooted. This algorithm leads to linear-time isomorphism algorithms for related graph classes, including Helly circular-arc graphs, $Gamma$ circular-arc graphs, proper circular-arc graphs and convex-round graphs.","palabras_clave":"circular-ones property|matrix isomorphism|circular-arc graphs|graph isomorphism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63922,"idioma_id":4,"titulo":"Normal Helly circular-arc graphs and its subclasses","resumen":"A Helly circular-arc model M=(C,A) is a circle C together with a Helly family A of arcs of C. If no arc is contained in any other, then M is a proper Helly circular-arc model, if every arc has the same length, then M is a unit Helly circular-arc model, and if there are no two arcs covering the circle, then M   is a normal Helly circular-arc model. A Helly (resp.&nbsp;proper Helly, unit   Helly, normal Helly) circular-arc graph is the intersection graph of   the arcs of a Helly (resp.&nbsp;proper Helly, unit Helly, normal Helly)   circular-arc model. In this article we study these subclasses of Helly   circular-arc graphs. We show natural generalizations of several   properties of (proper) interval graphs that hold for some of these Helly   circular-arc subclasses. Next, we describe characterizations for the   subclasses of Helly circular-arc graphs, including forbidden induced   subgraphs characterizations. These characterizations lead to efficient   algorithms for recognizing graphs within these classes. Finally, we show   how these classes of graphs relate with straight and round digraphs.","palabras_clave":"Helly circular-arc graphs|Proper circular-arc graphs|Unit circular-arc graphs|Normal circular-arc graphs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63923,"idioma_id":4,"titulo":"Antihistaminergics and inverse agonism. Potential therapeutic applications","resumen":"The accurate characterization of the molecular mechanisms involved in the action of receptor ligands is important for their appropriate therapeutic use and safety. It is well established that ligands acting at the histamine system currently used in the clinic exert their actions by specifically antagonizing G-protein coupled H1 and H2 receptors. However, most of these ligands, assumed to be neutral antagonists, behave as inverse agonists displaying negative efficacy in experimental systems. This suggests that their therapeutic actions may involve not only receptor blockade, but also the decrease of spontaneous receptor activity. The mechanisms whereby inverse agonists achieve negative efficacy are diverse. Theoretical models predict at least three possible mechanisms, all of which are supported by experimental observations. Depending on the mechanism of action engaged, the inverse agonist could interfere specifically with signaling events triggered by unrelated receptors. This possibility opens up new venues to explain the therapeutic actions of inverse agonists of the histamine receptor and perhaps new therapeutic applications.","palabras_clave":"ANTIHISTAMINERGIC DRUGS|INVERSE AGONIST|HISTAMINE|THERAPEUTIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63924,"idioma_id":4,"titulo":"Knowledge Representation of the Software Architecture Design Process based on Situation Calculus","resumen":"Software architecture design is an interactive, complex, decision-making process. Such a design process involves the exploration, evaluation, and composition of design alternatives. Increasingly, new computer-aided tools are available to help designers in these complex activities. However, these tools do not know how design is actually done, in other words, by means of which design activities the final artefact was obtained. In fact, the architectural design knowledge exclusively rests in the mind of designers, and there is an urgent need to move it, as much as possible, to a computer-supported environment that enables the capture of this type of knowledge. This contribution addresses this need by introducing a model for capturing how products under development are generated and transformed along the software architecture design process. The proposed model follows an operational perspective, where architectural design decisions are modelled by means of sequences of operations that are applied on the design products. Situation calculus is used to formally express the existence of an object in a given state of a design process. In addition, this formalism allows us expressing without ambiguities when an operation can be performed in a specific state of the design process.evaluation, and composition of design alternatives. Increasingly, new computer-aided tools are available to help designers in these complex activities. However, these tools do not know how design is actually done, in other words, by means of which design activities the final artefact was obtained. In fact, the architectural design knowledge exclusively rests in the mind of designers, and there is an urgent need to move it, as much as possible, to a computer-supported environment that enables the capture of this type of knowledge. This contribution addresses this need by introducing a model for capturing how products under development are generated and transformed along the software architecture design process. The proposed model follows an operational perspective, where architectural design decisions are modelled by means of sequences of operations that are applied on the design products. Situation calculus is used to formally express the existence of an object in a given state of a design process. In addition, this formalism allows us expressing without ambiguities when an operation can be performed in a specific state of the design process.","palabras_clave":"DESIGN PROCESS REPRESENTATION|SOFTWARE ARCHITECTURES|SITUATION CALCULUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63925,"idioma_id":4,"titulo":"Photo- radio- and thermoluminescence of Sm3+ doped and Tb3+\/Sm3+ doubly doped K2YF5 single crystals","resumen":"Photoluminescence, radioluminescence and thermoluminescence properties of K2YF5 crystals containingdifferent concentrations of samarium and terbium ions have been investigated for the first time. It has beenfound that the emission peaks observed in ranges from 300 to 550 nm and from 550 to 750 nm can beattributed to the 4f\u20134f transitions of the Tb3+ ions and the Sm3+ ions, respectively, for both photoluminescence and radioluminescence. The maximum of light output for radioluminescence under beta irradiationhas been obtained for K2YF5 crystals doped with 1.0 at % Sm3+ ions and the prospects for using suchdosimeter sin fiber optic dosimetry have been evaluated. On the other hand, it has been found that the glowcurves of doubly doped fluoride crystals are made up of at least four overlapping glow peaks and thatK2YF5:1.0 at % Tb3+; 0.5 at % Sm3+ crystals show the most intense thermoluminescence emission.","palabras_clave":"RARE-EARTH DOPED K2 YF5|PHOTOLUMINESCNENCE|RADIOLUMINESCENCE|THERMOLUMINESCENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63926,"idioma_id":4,"titulo":"Antiparasitic hybrids of Cinchona alkaloids and bile acids","resumen":"A series of 16 hybrids of Cinchona alkaloids and bile acids (4a-h, 5a-h) was prepared  by means of a Barton-Zard decarboxylation reaction. Quinine, quinidine, cinchonine  and cinchonidine were functionalized at position C-2 of the quinoline nucleus by radical  attack of a nor-cholane substituent. The newly synthesized hybrids were evaluated in  vitro for their antitrypanosomal, antileishmanial and antiplasmodial activities, along  with their cytotoxicity against WI38, a normal human fibroblast cell line. Seven  compounds (4d, 4f, 4h, 5b, 5d, 5f, 5h) showed promising trypanocidal activity with  IC50 values in the same range as the commercial drug suramine. Moreover all the 16  hybrids showed antiplasmodial activity (IC50 &#8804; 6 &#956;g\/ml), particularly those containing a  nor-chenodeoxycholane moiety (4b, 4d, 4f, 4h, 5b, 5d, 5f, 5h) with IC50 values  comparable to those of the natural alkaloids, and selectivity indices in the range of 5.6-  15.7.","palabras_clave":"CINCHONA ALKALOIDS|BILE ACIDS|BARTON-ZARD REACTION|ANTIPARASITIC ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63927,"idioma_id":4,"titulo":"Analysis of the main dosimetric peak of Al2O3:C compounds with a model of interacting traps","resumen":"The glow curve of Al2O3:C compounds has been analyzed by employing a model consisting of two active traps, thermally disconnected traps and one recombination centre.The analysis takes into account interaction among traps and the thermal quenching of the thermoluminescent emission.","palabras_clave":"THERMOLUMINESCENCE|GLOW CURVE ANALYSIS|INTERACTING TRAPS|Al2O3:C"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63928,"idioma_id":4,"titulo":"Growth, abundance, morphometric and metabolic parameters of three populations of Diplodon chilensis subject to different levels of natural and anthropogenic organic matter input in a glaciar lake of North Patagonia","resumen":"Three populations of Diplodon chilensis (Hiridae, Bivalvia) from North Patagonia (Lacar lake, Argentina) have been studied to determine how natural and anthropogenic organic matter provided by the forest or urban discharges may influence their growth, density morphometric and metabolic parameters. These populations are exposed to different levels of natural organic matter, due to different annual precipitation rates (sites Yuco and Nonthué), or to increased organic matter associated to urban discharges (San Martín de los Andes Bay, site  SMA). In Nonthué, a dense neighboring forest provides higher quantities of vegetal detritus compared to Yuco, while in SMA the organic matter (OM) input increase is related to anthropogenic impact (sewage discharges). Our results show that size distribution and growth rate are affected by increased OM, independently of its natural or anthropogenic origin. The modal shell length interval for both SMA and Nonthué is two-fold higher (70 mm), in   agreement to the growth rate increase (k=0.079),  compared to Yuco (35 mm, k=0.045), but only SMA bivalves show lower maximum age and density.  No individuals under 7 years old have been found in this population. Additionally, we have detected an increase in lipid peroxidation levels and in the defense\/detoxification enzyme glutathione-S-transferase (GST)   activity in SMA bivalves, respect to the other two populations. The differences in OM quality, reflected  in the DBO\/DQO  ratio, plus the evident eutrophic process taking place in SMA during  the  last decade are reflected in  population and  oxidative  stress parameters. The   absence of young individuals in this site suggests that juvenile mussels are unable to survive under these conditions and\/or recruitment to the population is impaired.","palabras_clave":"FRESHWATER BIVALVES|GROWTH RATES|POPULATION PARAMETERS|ALLOCHTHONOUS ORGANIC MATTER|ANTHROPOGENIC IMPACT|SEWAGE POLLUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63929,"idioma_id":4,"titulo":"Retinoic Acid Promotes Apoptosis and Differentiation in Photoreceptors by Activating the P38 MAP Kinase Pathway","resumen":"PURPOSE. Retinoic ?1 acid (RA) has a critical role during development of the retina. We  investigated RA effects on photoreceptor apoptosis and differentiation, and the intracellular  pathways involved.  METHODS. Rat retinal neuronal cultures were supplemented with RA with or without  docosahexaenoic acid (DHA), a photoreceptor survival factor, and photoreceptor apoptosis  and differentiation were evaluated at different times of development. To investigate the  intracellular pathways activated by RA, the levels of phosphorylated (P) ERK and P-p38 in  cultures with or without RA, and the effect of pretreatment with SB203580, a p38 specific  inhibitor, on apoptosis and differentiation were evaluated.  RESULTS. RA addition at day 0, when cells still were proliferating, selectively increased  apoptosis in photoreceptors, whereas addition at day 2 no longer caused cell death. RA  stimulated opsin and peripherin expression, and neurite outgrowth regardless of the time of  development. Addition of RA at day 0, but not at day 2, rapidly increased P-p38 levels, but did  not affect P-ERK levels. p38 inhibition completely prevented RA-induced apoptosis, and  partially decreased differentiation. DHA prevented apoptosis and additively increased  differentiation, without affecting RA activation of p38.  CONCLUSIONS. Our results show that RA activation of the p38 intracellular pathway was  essential for its early induction of apoptosis and partially responsible for promoting  differentiation. DHA prevention of this ?2 apoptosis suggests that RA effects during early  development must be counterbalanced by survival factors ?3 to prevent photoreceptor death, in  an interplay that might help to establish the final number of photoreceptors. ?4  Keywords: photoreceptors, retinoid, apoptosi","palabras_clave":"photoreceptor|retinoic|apoptosis|retina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63930,"idioma_id":4,"titulo":"Real-time monocular image based path detection","resumen":"In this work we present a new autonomous navigation method.  This method allows a mobile robot equipped with a monocular camera to travel through different naturally delimited paths.  It does not require camera calibration or a priori given knowledge of the environment.  The core of the method is based on segmenting images and classifying each super-pixel to infer a contour of navigable space.  The contour shape is used to calculate the forward and steering speed of the mobile robot.  To achieve real-time response of the robot, the image segmentation is implemented on a low-power embedded GPU.  The validity of our approach has been verified with an image dataset of various outdoor paths as well as with a real mobile robot.","palabras_clave":"Visual navigation|Path detection|Mobile robots"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63931,"idioma_id":4,"titulo":"A new species of Havelockia Pearson, 1903 from the Argentine Sea (Holothuroidea: Dendrochirotida: Sclerodactylidae)","resumen":"Havelockia pegi sp. nov., is here described from shallow waters of the Argentine Sea. This new species is distinctive in the purple colouration of its tentacles, scarcity of body wall ossicles and the presence of rosette-shaped ossicles in both the introvert and the tentacles. It is not closely related to any of its congenors. This is the first record of a true sclerodactylid from Argentina. Thandarum hernandezi Martinez &amp; Brogger, 2012, described in the family Sclerodactylidae, is now classified in the family Sclerothyonidae.","palabras_clave":"NEW SPECIES|HAVELOCKIA|ARGENTINE SEA|ECHINODERMATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63932,"idioma_id":4,"titulo":"Severe phosphorus stress affects sunflower and maize but not soybean root to shoot allometry","resumen":"The predictions of the two models on biomass allocation were compared on P-stressed and non-stressed crop plants. Allometriccoefficients were calculated from paired measurements of root and shoot biomass obtained from field and greenhouse experimentswith soybean [Glycine max (L.) Merr.], sunflower (Helianthus annuus L.), and maize (Zea mays L.) plants. Soybean consistentlyfollowed the allometric model, with the allocation pattern governed by the plant size (common slope K of 0.96 and 0.82 in thefield and greenhouse, respectively). Sunflower and maize showed allometric trajectories in the field but optimal partitioningtrajectories in the greenhouse. Field data for sunflower and maize adjusted to a unique line (K = 0.92 and 1.05, respectively)indicating that the biomass allocation is explained by allometric trajectories irrespective of the P level. In contrast, greenhouse data adjusted to two parallel lines (i.e., different elevation coefficient but similar slope: 0.91 for sunflower, 0.96 for maize). Only under severe P stress sunflower and maize plants modified their allocation pattern. Since the severity of the P stress needed to induce a shift in the allocation pattern would be large, we conclude that the three species follow unique root to shoot allometric trajectories under P levels usually found in the field. Most studies analyze the slope of the allometric relationships irrespective of the intercept. Here, we show that only the joint analysis of the slope and the elevation helps understand the effect of P availability on the biomass allocation pattern of relevant crop species.","palabras_clave":"Phosphorus|soybean|sunflower|maize"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63933,"idioma_id":4,"titulo":"Enterococcus faecalis utilizes maltose by connecting two incompatible metabolic routes via a novel maltose 6\u0002-phosphate phosphatase (MapP)","resumen":"Similar to Bacillus subtilis, Enterococcus faecalis transports and phosphorylates maltose via a phos-phoenolpyruvate (PEP):maltose phosphotransferasesystem (PTS). The maltose-speci&#64257;c PTS permease is encoded by the malT gene. However, E. faecalis lacks a malA gene encoding a 6-phospho-a-glucosidase, which in B. subtilis hydrolyses maltose 6-P into glucose and glucose 6-P. Instead, an operon encoding a maltose phosphorylase (MalP), a phosphogluco-mutase and a mutarotase starts upstream from malT. MalP was suggested to split maltose 6-P into glucose1-P and glucose 6-P. However, puri&#64257;ed MalP phospho-rolyses maltose but not maltose 6-P. We discovered that the gene downstream from malT encodes a novel enzyme (MapP) that dephosphorylates maltose 6-P formed by the PTS. The resulting intracellular maltose is cleaved by MalP into glucose and glucose 1-P. Slow uptake of maltose probably via a maltodextrin ABCtransporter allows poor growth for the mapP but not the malP mutant. Synthesis of MapP in a B. subtilis mutant accumulating maltose 6-P restored growth on maltose. MapP catalyses the dephosphorylation of intracellular maltose 6-P, and the resulting maltose is converted by the B.subtilis maltose phosphorylase into glucose and glucose 1-P. MapP therefore connects PTS-mediated maltose uptake to maltose phosphorylase-catalysed metabolism. Dephosphorylation assays with a wide variety of phospho-substrates revealed that MapP preferably dephosphorylates disaccharides containing an O-a-glycosyl linkage.","palabras_clave":"Maltose Metabolism|Enterococcus faecalis|maltose 6\u0002-phosphate phosphatase (MapP)|lactic acid bacterium"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63934,"idioma_id":4,"titulo":"Improved risk assessment and risk reduction strategies in the Water Safety Plan (WSP) of Salta, Argentina","resumen":"The Water Safety Plan (WSP) for the city of Salta (Argentina) is presented and discussed. The WSP was developed following the methodology proposed by the World Health Organization (WHO) adapted to local circumstances. Changes included a preliminary weighting procedure to assess the relative importance of different parts of the system, and a more systematic estimation of the magnitude of control measures. These modifications allowed the definition of a variety of riskreduction strategies. The risk assessment step was performed during participatory workshops withmembers of the water company. Initial risk for the entire system was 30.2%, with some variationsamong processes, sub-processes and components. More than 60% of the hazardous situationsidentified require some kind of control measure, assuming an acceptable risk threshold of 24%. Ifall control measures were successfully implemented, the final risk could be lowered to 17.7%.Methodological changes introduced allowed a more detailed analysis of the risks and can be animportant improvement of the original assessment method.","palabras_clave":"ARGENTINA|RISK ASSESSMENT|SALTA|WATER SAFETY PLAN (WSP)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63935,"idioma_id":4,"titulo":"Intertwined arbovirus transmission activity: reassessing the transmission cycle paradigm","resumen":"Arbovirusesareemerging\/reemerginginfectiousagentsworldwide.Thefactorswithinthisscenarioincludevectorandhostpopulationfluctuations,climaticchanges,anthropogenicactivitiesthatdisturbecosystems,anincreaseininternationalflights,humanmobility,andgeneticmutationsthatallowspill-overphenomenon.Arbovirusesaremaintainedbybiologictransmissionamongvectorsandhosts.SometimesthisbiologicaltransmissionisspecificandincludesonevectorandhostspeciessuchasChikungunya(CHIKV),Dengue(DENV),andurbanYellowFever(YFV).However,mostofthearbovirusesaregeneralistandtheyusemanyvectorsandhostsspecies.Fromthisperspective,arbovirusesaremaintainedthroughatransmissionnetworkratherthanatransmissioncycle.Thisallowsustounderstandthecomplexityanddynamicsofthetransmissionandmaintenanceofarbovirusesintheecosystems.Theoldperspectivethatarbovirusesaremaintainedincloseandstabletransmissioncyclesshouldbemodifiedbyanewmoreintegrativeanddynamicia,representingtherealscenariowherebiologicalinteractionshaveamuchbroaderrepresentation,indicatingtheconstantadaptabilityofthebiologicalentities.","palabras_clave":"Arbovirus|St.Louisencephalitisvirus|transmissioncycles|WestNilevirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63936,"idioma_id":4,"titulo":"Unsaturated Long Chain Free Fatty Acids Are Input Signals of the Salmonella enterica PhoP\/PhoQ Regulatory System","resumen":"The Salmonella enterica serovar Typhimurium PhoP\/PhoQ system has largely been studied as a paradigmatic two-component regulatory system not only to dissect structural and functional aspects of signal transduction in bacteria, but also to gain knowledge about the versatile devices that have evolved allowing a pathogenic bacterium to adjust to or counteract environmental stressful conditions along its life cycle. Mg2+ limitation, acidic pH and the presence of cationic antimicrobial peptides have been identified as cues that the sensor protein PhoQ can monitor to reprogram Salmonella gene expression to cope with extra- or intra-cellular challenging conditions. In this work, we show for the first time that long chain unsaturated free fatty acids (LCUFAs) present in Salmonella growth medium are signals specifically detected by PhoQ. We demonstrate that LCUFAs inhibit PhoQ autokinase activity, turning off the expression of the PhoP-dependent regulon. We also show that LCUFAs exert their action independently of their cellular uptake and metabolic utilization by means of the ß-oxidative pathway. Our finding puts forth the complexity of input signals that can converge to finely tune the activity of the PhoP\/PhoQ system. Besides, it provides a new potential biochemical platform for the development of antibacterial strategies to fight against Salmonella infections.","palabras_clave":"Salmonella|Sistema PhoP\/PhoQ|Saponificacion|Ácidos grasos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63937,"idioma_id":4,"titulo":"Highly selective copper nanoparticles for the hydrogenation of\u0002,\u0003-unsaturated aldehydes in liquid phase","resumen":"Unsupported copper nanoparticles (CuNPs), 3\u20134 nm in size, are obtained from the reduction of the cor-responding metal chloride through an electron transfer from a lithium arenide. CuNPs are tested for theliquid phase hydrogenation of cinnamaldehyde in batch conditions by using H2. High activity and selec-tivity to cinnamyl alcohol (87%, at high conversion) are reached. When CuNPs are supported on MCM-41and on high surface area CeO2, TEM and XRD characterization shows that the nanometric size of cop-per is retained. For the hydrogenation of crotonaldehyde or of cinnamaldehyde, CuNPs\/MCM-41 andCuNPs\/CeO2are less active and less selective than unsupported nanoparticles. However the selectivitylevels reached by these catalysts are higher than those obtained over noble metal (Au and Pt) based cata-lysts, tested under the same reaction conditions. The catalytic properties of supported and unsupportedCuNPs would be related to the nanometric size of copper","palabras_clave":"copper nanoparticles|ceria|selective hydrogenation|crotonaldehyde"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63938,"idioma_id":4,"titulo":"Na+, K+-ATPase response to neurotensin is altered by streptozotocin administration","resumen":"Neurotensin is a basic tridecapeptide which can act as a neuromodulator or a neurotransmitter, and binds to a group of receptors.Neurotensin is able to inhibit Na\/K-ATPase activity, an effect blocked by the presence of antagonist SR48692, suggesting the involvement of high affinity neurotensin (NTS1) receptor. Diverse evidences suggest a relationship between neurotensinergic system and glycemia levels. For this reason, Na\/K-ATPase regulation by neurotensin in brain membranes obtained from rats turned hyperglycaemic was explored. As a model to produce diabetes mellitus rats were administered with Streptozotocin (STZ), a specific toxic to the pancreatic beta cells. Present findings indicated that Na\/K-ATPase activity in synaptosomal membranes isolated from diabetic rats failed to respond to neurotensin. The treatment decreased the affinity of NTS1 receptor for neurotensin and the expression of Na\/K-ATPase alpha3 isoform in cerebral cortex. Results led us to conclude that STZ administration alters the response of Na\/K-ATPase to neurotensin. Such effect","palabras_clave":"Na+, K+-ATPase |Neurotensin|Streptozotocin|Diabetes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63939,"idioma_id":4,"titulo":"Development and validation of a stability indicating method for seven novel derivatives of lamivudine with activity anti-HIV and anti-HBV in simulated gastric and intestinal fluids","resumen":"A simple micellar liquid chromatography (MLC) method has been developed and validated for use in stability indicating studies of lamivudine and its carbonate derivatives with proved activity against human immunodeficiency and hepatitis B viruses (HIV and HBV, respectively), in simulated gastric (SGF) and intestinal (SIF) fluids samples. The optimized method involves a C18 column thermostated at 30 &#9702;C, UV detection at 272 nm, a flow rate of 1.0 mL min&#8722;1 and a micellar mobile phase composed by 0.15 M sodium dodecyl sulphate (SDS) \u2013 4% (v\/v) 1-butanol \u2013 0.01 M KH2PO4\u2013Na2HPO4 (pH 7), using zidovudine (AZT) as internal standard. Validation under Food and Drug Administration (FDA) guideline of the analytical parameters include: linearity (r2 &gt; 0.9996), LODs (1.6 × 10&#8722;7\u20136.9 × 10&#8722;6 M) and LOQ (1 × 10&#8722;5 M), intra (0.02\u20131.48%) and inter-day precision (0.04\u20131.66%) expressed as relative standard deviation (R.S.D.), and robustness parameters (less than 1.98%). Using this method, recoveries ranging from 92.9 to 119% were obtained for the eight substances. Thus, this method provides a simple, sensitive, accurate and precise assay for the determination of all compounds that can be readily adaptable to routine use by clinical laboratories with standard equipment. In addition, we evaluated the stability of carbonates of lamivudine in buffer pH 1.2 and 6.8; SGF (pH 1.2) and SIF one (pH 6.8), all as indicated in United States Pharmacopeia (USP) 32. Finally, this chromatographic method was applied to stability studies which resulted in all the compounds following a pseudo-first-order kinetics, and in the determination of its kinetic constant and half-life time. &#9702;C, UV detection at 272 nm, a flow rate of 1.0 mL min&#8722;1 and a micellar mobile phase composed by 0.15 M sodium dodecyl sulphate (SDS) \u2013 4% (v\/v) 1-butanol \u2013 0.01 M KH2PO4\u2013Na2HPO4 (pH 7), using zidovudine (AZT) as internal standard. Validation under Food and Drug Administration (FDA) guideline of the analytical parameters include: linearity (r2 &gt; 0.9996), LODs (1.6 × 10&#8722;7\u20136.9 × 10&#8722;6 M) and LOQ (1 × 10&#8722;5 M), intra (0.02\u20131.48%) and inter-day precision (0.04\u20131.66%) expressed as relative standard deviation (R.S.D.), and robustness parameters (less than 1.98%). Using this method, recoveries ranging from 92.9 to 119% were obtained for the eight substances. Thus, this method provides a simple, sensitive, accurate and precise assay for the determination of all compounds that can be readily adaptable to routine use by clinical laboratories with standard equipment. In addition, we evaluated the stability of carbonates of lamivudine in buffer pH 1.2 and 6.8; SGF (pH 1.2) and SIF one (pH 6.8), all as indicated in United States Pharmacopeia (USP) 32. Finally, this chromatographic method was applied to stability studies which resulted in all the compounds following a pseudo-first-order kinetics, and in the determination of its kinetic constant and half-life time. ","palabras_clave":"ESTABILIDAD|CROMATOGRAFÍA LÍQUIDA MICELAR|CARBONATOS DE LAMIVUDINA|ACTIVIDAD ANTI HIV|ACTIVIDAD ANTI  HBV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63940,"idioma_id":4,"titulo":"Galacturonosyltransferase 4 silencing alters pectin composition and carbon  partitioning in tomato","resumen":"-","palabras_clave":"FRUIT METABOLISM|GALACTURONIC ACSI|GALACTURONOSYLTRANSFERASE|PECTIN|SOLANUM PENNELLI|TOMATO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63941,"idioma_id":4,"titulo":"Anticoagulant activity of a unique sulfated pyranosic (1,3)-&#946;-L-Arabinan through direct interaction with thrombin","resumen":"A highly sulfated 3-linked &#946;-arabinan (Ab1) with arabinose in the pyranose form was obtained from green seaweed Codium vermilara (Bryopsidales). It comprised major amounts of units sulfated on C-2 and C-4 and constitutes the first polysaccharide of this type isolated in the pure form and fully characterized. Ab1 showed anticoagulant activity by global coagulation tests. Less sulfated arabinans obtained from the same seaweed have less or no activity. Ab1 exerts its activity through direct and indirect (antithrombin- and heparin cofactor II-mediated) inhibition of thrombin. Direct thrombin inhibition was studied in detail. By native PAGE, it was possible to detect formation of a complex between Ab1 and human thrombin (HT). Ab1 binding to HT was measured by fluorescence spectroscopy. CD spectra of the Ab1 complex suggested that ligand binding induced a small conformational change on HT. Ab1-thrombin interactions were studied by molecular dynamic simulations using the persulfated octasaccharide as model compound. Most carbohydrate-protein contacts would occur by interaction of sulfate groups with basic amino acid residues on the surface of the enzyme, more than 60% of them being performed by the exosite 2-composing residues. In these interactions, the sulfate groups on C-2 were shown to interact more intensely with the thrombin structure. In contrast, the disulfated oligosaccharide does not promote major conformational modifications at the catalytic site when complexed to exosite 1. These results show that this novel pyranosic sulfated arabinan Ab1 exerts its anticoagulant activity by a mechanism different from those found previously for other sulfated polysaccharides and glycosaminoglycans.","palabras_clave":"arabinopyranose|green seaweed|sulfated arabinan|anticoagulant activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63942,"idioma_id":4,"titulo":"Alterations of fibrin network structure mediated by dermatan sulfate","resumen":"Dermatan sulfate (DS) is well-known for its anticoagulant activity through binding to heparin cofactor II (HCII) to enhance thrombin inhibition. It has also been reported that DS has a profibrinolytic effect. We have evaluated the effects of DS solutions (4-20 &#956;g\/mL) on the formation (by kinetic studies), structure (by electron microscopy and compaction assays) and lysis (with urokinase-type plasminogen activator) of plasma fibrin networks. The results showed that DS significantly prolonged the lag phase and decreased the fibrin formation rate and the optical density of the final networks versus control, in a concentration dependent way. DS-associated networks presented a minor network percentage compared with control, composed of lower number of fibers per field, which resulted significantly thinner and longer. Moreover, DS rendered gels more sensible to rupture by centrifugal force and more susceptible to lysis. When fibrin formation kinetic assays were performed with purified fibrinogen instead of plasma, in the absence of HCII, the optical density of final DS-associated networks was statistically lower than control. Therefore, a direct effect of DS on the thickness of fibers was observed. Since in all in vitro assays low DS concentrations were used, it could be postulated that the fibrin features described above are plausible to be found in in vivo thrombi and therefore, DS would contribute to the formation of less thrombogenic clots","palabras_clave":"dermatan sulphate|fibirn networks"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63943,"idioma_id":5,"titulo":"Sistema acosador y subsistemas asociados a la violencia laboral","resumen":"Con este trabajo nos proponemos presentar una red categorial y su proceso de construcción como instrumento para el análisis cualitativo de datos en un proyecto de investigación que tiene como área de problematización las condiciones, formas y estructuración de un sistema acosador en el ámbito laboral.A partir del estudio de los procesos de hostigamiento, se focalizó la indagación en los incidentes críticos como situación puntual y disruptiva en los que se origina el acoso. El análisis para detectar la emergencia del incidente crítico a partir del modelo relacional de redes semánticas permitió visualizar las combinaciones entre distintos subsistemas dentro del sistema acosador, profundizando en la comprensión de los procesos de emergencia del acoso y abriendo posibilidades de intervención institucional temprana para modificar la situación.","palabras_clave":"Incidente crítico|análisis de contenido|-"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63944,"idioma_id":4,"titulo":"Modulation of the immuno-coagulative response in a pneumococcal infection in malnourished mice nasally treated with lactobacillus casei","resumen":"We studied the systemic effects of the intranasal administration of Lactobacillus casei on the immuno-coagulative response in pneumoccocal infection in immunocompromised mice. Weaned mice consumed a protein-free diet (PFD) for 21 days and were therefore malnourished. Malnourished mice were fed a balanced conventional diet (BCD) for 7 days (BCD group) or a BCD for 7 days with nasal administration of viable L. casei on days 6 and 7 (BCD+LcN group). The malnourished control mice (MNC) received a PFD, whereas the well-nourished control mice (WNC) continually consumed a BCD. At the end of the treatment period, the mice were infected with Streptococcus pneumoniae. At different times after infection, we analysed the following parameters: global coagulation system, activation of coagulation, coagulation inhibitors, platelet count, leukocyte count and myeloperoxidase (MPO) activity, total proteins, albumin and acute phase proteins (APPs). The MNC group showed greater impairment in the coagulation tests and an increase in the positive APPs. These parameters were normalized by the L. casei treatment. However, the number of leukocytes, decreased by malnutrition, was improved only by the administration of L. casei. After infection, the BCD+LcN group showed similar results to those of the WNC group for most of the haemostatic parameters. The BCD+LcN group did not show significant variations in the prothrombin time or in the level of anticoagulant protein C, but showed higher levels of fibrinogen, platelets, albumin, leukocytes and MPO activity compared with the different experimental groups. The intranasal administration of L. casei was effective in modulating the pro-inflammatory aspects of coagulation without affecting coagulation itself.","palabras_clave":"Acute-Phase Proteins|Blood Coagulation|Lactobacillus casei|Malnutrition"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63945,"idioma_id":4,"titulo":"Photometric and spectroscopic variations of the Be star HD 112999","resumen":"General Be objects are stars of B spectral type showing lines of the Balmer series in emission. The presence of these lines is attributed to the existence     of an extended envelope, disk type, around them. Some stars are observed in     both the Be and normal B-type spectroscopic states and they are known as  transient Be stars. In this paper we show the analysis carried out on a new possible transient Be star, labelled HD 112999, using spectroscopic optical observations  and  photometric data.","palabras_clave":"Stars: emission-line, Be|Stars: individual: HD 112999|Stars: variables"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63946,"idioma_id":5,"titulo":"Variables socio-organizacionales y sociodemográficas de alto impacto en los procesos de acoso laboral","resumen":"El presente trabajose centra en indagar el papel de la ?variable ambiente? y las condiciones sociodemográficasde los trabajadores en la predicción de los procesos de acoso laboral. Tanto lascondiciones del trabajo como las de su organización y las características delos grupos de trabajadores son ubicadas por algunas investigaciones comoelementos que contribuyen a la emergencia de situaciones de acoso laboral.Éstas se diferencian a su vez de estudios de precursores basados en las fuentesinteraccionales y en las estructuras de personalidad. El objetivo de este trabajo es presentar losresultados vinculados a la exploración e identificación de factoresorganizacionales y sociodemográficos asociados a la emergencia de situacionesde acoso laboral. Se indagaron las siguientes variables y surelación con el acoso laboral: edad, sexo, nivel de estudios, tipo y tamaño dela organización, trayectoria laboral, antigüedad, tipo de contrato, autotipificacióncomo víctima de acoso y tenencia de gente a cargo. Se trabajó con un análisis de regresión múltiple porel método de pasos sucesivos, detectándose relación en tamaño de laorganización, posición jerárquica, tipo de organización, nivel de estudios,trayectoria laboral y edad.Se analizan estasvariables al interior de un modelo que las reagrupa en habilitantes,motivadoras y catalizadoras","palabras_clave":"factores predictores|acoso laboral|variable ambiente|variables sociodemográficas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63947,"idioma_id":5,"titulo":"Percepción de anomia, confianza y bienestar: la mirada desde la psicología social","resumen":"La presente investigación busca conocer la percepción de frustración anómica, confianza institucional y bienestar social de los sujetos así como la relación entre estos indicadores. El estudio es descriptivo correlacional, de diseño no experimental transversal, con una muestra no probabilística intencional compuesta por 585 participantes (34.7% hombres y 65.3% mujeres. Edad: M = 28.50; SD = 11.02) de Buenos Aires y otras ciudades de Argentina. Los resultados arrojan una percepción positiva en el bienestar social con un déficit en la confianza en los otros, bajos niveles de confianza en las instituciones y niveles altos de frustración anómica. Se corrobora la validez convergente y surgen diferencias a partir de variables de agrupación.","palabras_clave":"ANOMIA|ALIENACIÓN|CONFIANZA (COMPORAMIENTO SOCIAL)|BIENESTAR|VALORES SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63948,"idioma_id":4,"titulo":"The main component of an alarm pheromone of kissing bugs plays multiple roles in the cognitive modulation of the escape response","resumen":"Innate responses in animals can be modulated by experience. Disturbed adults of the triatomine bug Triatoma infestans release an alarm pheromone (AP) that elicits an escape response in conspecific larvae. The main component of this AP, the isobutyric acid (IsoAc), alone has already shown to generate an escape response in this species. However, not much is known about the modulation of this behavior by non-associative and associative cognitive processes. We present here evidences of the cognitive capacities of T. infestans larvae in an escape context under different conditioning paradigms, including IsoAc in different roles. We show that: 1) the duration of a pre-exposure to IsoAc plays a main role in determining the type of non-associative learning expressed: short time pre-exposures elicit a sensitization while a longer pre-exposure time triggers a switch from repellence to attractiveness; 2) a simple pre-exposure event is enough to modulate the escape response of larvae to the AP and to its main component: IsoAc; 3) IsoAc and the AP are perceived as different chemical entities; 4) an association between IsoAc and an aversive stimulus can be created under a classical conditioning paradigm; 5) an association between IsoAc and a self-action can be generated under an operant conditioning. These results evince that IsoAc can attain multiple and different cognitive roles in the modulation of the escape response of triatomines and show how cognitive processes can modulate a key behavior for surviving, as it is the escaping response in presence of a potential danger in insects. Aclaración que figura en la publicación: Los 2 primeros autores contribuyeron de igual manera en el trabajo (es decir, primera autoría compartida).","palabras_clave":"APRENDIZAJE|ENTOMOLOGIA|COMPORTAMIENTO|VINCHUCAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63949,"idioma_id":4,"titulo":"Dynamics of thin walled composite beams: Analysis of parametric uncertainties","resumen":"This article is concerned with the stochastic dynamic analysis of structures constructed with composite materials. Depending on many aspects (manufacturing process, material uncertainty, boundary conditions, etc.) real composite structures may have deviations with respect to the calculated response (or deterministic response). These aspects lead to a source of uncertainty in the structural response associated with constituent proportions, geometric parameters or other unexpected agents. Uncertainties should be considered in a structural system in order to improve the predictability of a given modeling scheme. In this study a model of shear deformable composite beams is employed as the mean model. The probabilistic model is constructed by adopting random variables for the uncertain parameters of the model. This strategy is called parametric probabilistic approach. The probability density functions of the random variables are constructed appealing to the Maximum Entropy Principle. The continuous model is discretised by finite elements and the Monte Carlo method is employed to perform the simulations, thereafter a statistical analysis is performed. Numerical studies are carried out to show the main advantages of the modeling strategies employed, as well as to quantify the propagation of the uncertainty in the dynamics of slender composite structures.","palabras_clave":"UNCERTAINTY QUANTIFICATION|THIN-WALLED BEAMS|DYNAMICS|COMPOSITE MATERIALS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63950,"idioma_id":4,"titulo":"Effects of thymol feed supplementation on female Japanese quail (Coturnix coturnix) behavioral fear response","resumen":"Dietary supplementation with thymol has been shown to improve the oxidative stability of eggs and meat during storage. In addition, in vitro studies have shown that this compound can act as a positive allosteric modulator of the GABAA receptor, similarly to its analogue phenolic compound propofol. Hence, it is conceivable that thymol could also present anxyolitic and\/or fear reducing properties, probably also affecting their locomotor activity. Considering that fearinducing\/ stressful situations are practically unavoidable during birds´ rearing, the thymol feed supplementation could therefore present beneficial consequences in terms of animal welfare. This study evaluates potential fear reducing properties of thymol feed supplementation and its potential effects on locomotor activity by assessing female Japanese quail behavioral responses during brief mechanical restraint and openfield tests. Birds were evaluated after 2 and 15 days of supplementation. During the brief mechanical restraints, the latencies to struggle were significantly (P<0.05) shorter and the number of struggling bouts  significantly higher (P<0.05) in the thymol group than in their Control  counterparts suggesting a fear reducing effect. No effects on locomotor  behavior were detected during open-field testing. The results suggest  that dietary supplementation with thymol may help reduce female fear responses when birds are exposed to stressful situation without affecting  the bird´s locomotor activity.","palabras_clave":"dietary supplementation|Japanese quail|thymol|restraint|open field"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63951,"idioma_id":5,"titulo":"Estilos parentales, inestabilidad emocional y agresividad en niños de nivel socioeconómico bajo en Argentina y España","resumen":"El objetivo es estudiar si el patrón de la relación entre el comportamiento de los padres y el desarrollo socioemocional de los niños se mantiene a través de diferentes contextos socioeconómicos y nacionales  (Argentina y España). Se administró la Escala Argentina de Percepción de la Relación con los Padres para Niños, elT TVerbal Aggression ScaleT Ty laT TEmotional InstabilityT TScaleT Ta 583 niños argentinos y españoles de 10 a 12 años. Los resultados indican: 1) la agresión y la inestabilidad emocional están relacionadas; 2) las dimensiones de estilos parentales se relacionan diferentemente con la inestabilidad emocional y la agresividad; 3) el comportamiento de la madre y del padre se relacionan en forma diferente con la regulación emocional de los niños; 4) los modelos psicológicos que asocian determinados comportamientos parentales con el desarrollo socioemocional de los niños, permanecen invariantes a través de los estratos económico-sociales y de las culturas nacionales estudiadas.","palabras_clave":"Estilos parentales -  Agresividad  |Inestabilidad emocional |Vulnerabilidad social-|Cultura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63952,"idioma_id":4,"titulo":"Description of the female of Aysha yacupoi Brescovit 1992 (Araneae: Anyphaenidae)","resumen":"The female of Aysha yacupoi Brescovit, 1992 is described and illustrated for the first time. Males and female specimens were observed together in the same plants. Some data on natural history are presented","palabras_clave":"Argentina|Atlantic rainforest|Ghost spider|Spider Taxonomy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63953,"idioma_id":5,"titulo":"La apropiación juvenil de las TIC. El contraste entre usuarios de cibercafé y usuarios hogareños","resumen":"En este trabajo se analizan los modos en que los jóvenes de sectores populares se apropian de la computadora e Internet, contrastando usuarios hogareños y usuarios de cibercafé. El análisis se basa en datos cuantitativos construidos mediante una encuesta realizada a jóvenes usuarios de TIC residentes en La Matanza en el año 2010.La encuesta indagó diversos temas relativos al vínculo de los jóvenes con las TIC: frecuencia y lugares de acceso, antigüedad de su experiencia con la computadora, habilidades informáticas y tipo de usos más frecuentes de la computadora e Internet.","palabras_clave":"JÓVENES|TIC|DESIGUALDADES|CIBERCAFÉS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63954,"idioma_id":4,"titulo":"Anthraquinones production in Rubia tinctorum cell suspension cultures: down scale of shear effects","resumen":"The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.","palabras_clave":"Anthraquinones|Rubia tinctorum|Shear stress|Down scale|Suspension cultures"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63955,"idioma_id":4,"titulo":"Structure Diversity of Nitric Oxide Synthases (NOS): The Emergence of New Forms in Photosynthetic Organisms","resumen":"Humans have enormously increased the level of nitrogen (N) circulating in the troposphere and the earth surface during the last century, correlating with the population increase. As an undesirable consequence, high levels of reactive N are polluting the environment where humans inhabit. Nitric oxide (NO) is one of the reactive N species with both positive and negative impact on life. NO synthases (NOS) are enzymes that oxidize arginine to citrulline and generate the denitrifying intermediate NO which can be subsequently reduced to N2O and N2. NOS are large modular enzymes present in all kingdoms which through evolution were the result of multiple gene and genome duplication events together with changes in protein architecture (Andreakis et al. 2011). A recently described NOS from the marine unicellular microalgae Ostreococcus tauri, belonging to the picoplankton in oceans, adds new insights to study the evolution of the complex organization of these enzymes. In this opinion we discuss the structure diversity of the emerging new NOS forms described in prokaryotes and eukaryotes. Regarding the controversy about the existence of canonical NOS in higher plants, we propose that the latest findings support the existence of a high diversity of NOS forms in different lineages. Thereby, since higher plant species whose genomes have been fully sequenced, which are scarce, it cannot be discarded that a new form of NOS may have evolved in higher plants.","palabras_clave":"NITRIC OXIDE SYNTHASE|PHOTOSYNTHETIC MICROORGANISM|OSTREOCOCCUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63956,"idioma_id":4,"titulo":"Auxin induces redox regulation of ascorbate peroxidase 1 activity by S-nitrosylation\/denitrosylation balance resulting in changes of root growth pattern in Arabidopsis","resumen":"S-nitrosylation of Cys-residues is one of the molecular mechanisms driven by nitric oxide (NO) for regulating biological functions of key proteins. While the studies on S-nitrosylation of Cys residues have served for identifying SNO-proteomes, the physiological relevance of protein S-nitrosylation\/denitrosylation remains poorly understood. In this study, it is shown that auxin influences the balance of S-nitrosylated\/denitrosylated proteins in roots of Arabidopsis seedlings. 2D-PAGE allowed the identification of ascorbate peroxidase 1 (APX1) as target of auxin-induced denitrosylation in roots. Auxin causes APX1 denitrosylation and partial inhibition of APX1 activity in Arabidopsis roots. In agreement, the S-nitrosylated form of recombinant APX1 expressed in E. coli is more active than the denitrosylated form. Consistently, Arabidopsis apx1 mutants have increased H2O2 accumulation in roots, shorter roots and less sensitivity to auxin than the wild type. It is postulated that an auxin-regulated counterbalance of APX1 S-nitrosylation\/denitrosylation contributes to a fine-tuned control of root development and determination of root architecture.","palabras_clave":"ASCORBATE PEROXIDASE 1|AUXIN|NITROSYLATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63957,"idioma_id":4,"titulo":"Polyphenol SiO2 hybrid biosorbent for heavy metal removal. Yerba mate waste (Ilex paraguariensis) as polyphenol source: Kinetics and isotherm studies","resumen":"  &amp;amp;amp;amp;amp;amp;amp;amp;lt;!--  @page { margin: 0.79in }  P { margin-bottom: 0.08in }  A:link { color: #0000ff }  --&amp;amp;amp;amp;amp;amp;amp;amp;gt;                  A  low cost biosorbent hybrid material ready for application was  obtained in this work. Yerba&nbsp; mate (Ilex  paraguariensis)  milling residual dust was used as a polyphenol source by ethanolic  extraction. Polyphenols were immobilized within a SiO2  matrix to form an interpenetrated polymer after glutaraldehyde  cross-linking. Pb(II), Cr(III) and Cr(VI) were chosen as model metals  for adsorption. The hybrid materials were  characterized by Fourier Transform Infrared Spectroscopy (FTIR),  Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray  Spectroscopy (EDS) and Nitrogen Adsorption Isotherms. Adsorption  experimental data were analysed using Langmuir, Freundlich,  Dubinin-Radushkevich, Temkin, Redlich-Peterson, Sips and Toth  isotherm models along with the evaluation of adsorption energy  and standard free energy (&#916;G0). The adsorption was  observed to be pH dependent. The main mechanism of metal adsorption  was found to be a spontaneous charge associated interaction. Electron  Spin Resonance spectroscopy (ESR) confirmed that Cr(VI) adsorption  was an adsorption-coupled reaction and the adsorbed specie was Cr(V).  The hybrid matrix probed its adsorption capacity of Cr(III) in a  non-treated tannery waste-water","palabras_clave":"Ilex paraguariensis |polyphenols|hybrid material|Cr(V)-Cr(VI)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63958,"idioma_id":4,"titulo":"Fermi surface instabilities at finite Temperature","resumen":" We present a new method to detect Fermi surface instabilities for interacting systems at finitetemperature. We first apply it to a list of cases studied previously, recovering already known results ina very economic way, and obtaining most of the information on the phase diagram analytically. As anexample, in the continuum limit we obtain the critical temperature as an implicit function of the magneticfield and the chemical potential Tc(&#956;, h). By applying the method to a model proposed to describe reentrantbehavior in Sr3Ru2O7 , we reproduce the phase diagram obtained experimentally and show the presence ofa non-Fermi Liquid region at temperatures above the nematic phase.  ","palabras_clave":"Fermi-liquid|Instabilities|Ruthenates|Finite-Temperature"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63959,"idioma_id":4,"titulo":"Two- versus three- dimensional morphometric approaches in macroevolution: insight from the mandible of caviomorph rodents.","resumen":"Morphological variation is one of the most studied dimensions of evolutionary biology, given its close relationship with the ecological aspects of biological diversification. In this work we examine the differences between the use of two- and three-dimensional morphometric techniques for the analysis of macroevolutionary patterns of morphological variation in the mandible of the South American caviomorph rodents, which displaysvarying degrees of hystricognathy. The variation patterns were similar for 2D and 3D coordinate datasets. However, phylogenetic comparative statistical analyses showedwidely different results for mandible variation. Both the phylogenetic signal values and the results of phylogenetic regressions were markedly different between the two macroevolutionary mandible datasets. This suggests that the inclusion of the third coordinate to characterize a hystricognathous mandible may lead to completely differentinterpretations concerning the processes that could be regulating the morphological diversification of the caviomorph mandible. We also compare these results with the2D and 3D macroevolutionary patterns of variation in cranial shape and the intra-specific mandible variation. The variation patterns were similar for 2D and 3D coordinatefor all these comparative datasets. The differing results obtained at different evolutionary scales, give strength to the statement about careful selection of data not only instudies of morphological variation in caviomorphs but also for other groups of organisms.","palabras_clave":"EVOLUTIONARY DIVERSIFICATION|GEOMETRIC MORPHOMETRICS|MANDIBLE MORPHOLOGY|SHAPE DIMENSIONALITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63960,"idioma_id":4,"titulo":"Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes","resumen":"This study demonstrates the capacity of the onestep polymerase chain reaction (PCR) fingerprinting method using the microsatellite primers (GACA)4 or (GTG)5 (MSPPCR) to identify six of the most frequent dermatophyte species causing cutaneous mycosis. PCR with (GACA)4 was a suitable method to recognise Microsporum canis, Microsporum gypseum, Trichophyton rubrum and Trichophyton interdigitale among 82 Argentinian clinical isolates, producing the  most simple and reproducible band profiles. In contrast, the identification of Trichophyton mentagrophytes and Trichophyton tonsurans was achieved using PCR with (GTG)5. In this way, the sequential application of PCR using (GACA)4 and (GTG)5 allowed the successful typification of clinical isolates which had not been determined by mycological standard techniques. In this work, the intraspecies variability among 33 clinical isolates of M. canis was detected using  random amplification of polymorphic DNA (RAPD-PCR) with the primers OPI-07 and OPK-20. The genetic variations in the isolates of M. canis were not associated with clinical features of lesions or pet ownership, but a geographical restriction of one genotype was determined with OPK-20, suggesting a clonal diversity related to different ecological niches in certain geographical areas. The results of this work demonstrate that the detection of intraspecies polymorphisms in M. canis by RAPD-PCR may be applied in future molecular epidemiological studies to identify endemic strains, the route of infection in an outbreak or the coexistence of different strains in a single infection","palabras_clave":"Dermatophytes|PCR fingerprinting|Molecular diagnostic|Molecular epidemiology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63961,"idioma_id":5,"titulo":"Enteroparasitosis en niños de Villaguay, Entre Ríos: Un estudio integrado al estado nutricional y al ambiente","resumen":"El objetivo del presente trabajo fue determinar la prevalencia de enteroparasitosis y su asociación con la desnutrición infantil y los factores socio-ambientales en el Departamento de Villaguay, Entre Ríos. Se realizó un estudio transversal en niños de ambos sexos con edades comprendidas entre 1 y 11 años incluidos en 2 grupos etarios (&#8804; de 5 y &#8805; de 6). Se analizaron muestras fecales y escobillados anales seriados y se relevaron peso y talla. Se estimaron bajo peso para la edad (BP\/E), baja talla para la edad (BT\/E) y bajo peso para la talla (BP\/T), utilizando como punto de corte el percentilo 5 de la referencia NHANES III. Se relevaron las condiciones socio-ambientales mediante encuestas estructuradas. Los datos fueron procesados con los programas Epi Info 2002 y SPSS 12.0. De los 268 niños analizados parasitológicamente, 58,6% resultaron positivos y de ellos el 12,1% presentaron poliparasitismo. Enterobius vermicularis (38,4%), Blastocystis hominis (27,2%) y Giardia lamblia (11,9%) fueron las especies más prevalentes. Las asociaciones más frecuentes entre pares de especies patógenas fueron B. hominis y E. vermicularis; B. hominis y G. lamblia, las que además se asociaron con especies no patógenas (i.e. Entamoeba coli, Endolimax nana). B. hominis y E. nana mostraron una prevalencia superior en las niñas y en los mayores de 6 años. El análisis del estado nutricional indicó mayor desnutrición crónica (7,1%) que global (0,9%) y aguda (0,5%). El 81,3% de los niños desnutridos resultó parasitado. Si bien la presencia de parásitos se asoció a familias con padres y madres sin trabajo estable y bajo nivel educativo (p<0,05), la desnutrición no se asoció con las variables socio-ambientales. Los resultados obtenidos indicarían el impacto de la precarización laboral y educativa de los padres en algunos sectores de la población de Villaguay, que conlleva a la coexistencia de las infecciones parasitarias y la desnutrición.","palabras_clave":"ENTEROPARASITOSIS|NIÑOS|VILLAGUAY, ENTRE RIOS|ESTADO NUTRICIONAL|AMBIENTE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63962,"idioma_id":4,"titulo":"Insights into panicoid inflorescence evolution","resumen":"Inflorescence forms can be described by different combinatorial patterns of meristem fates (indeterminate versus determinate). In theory, the model predicts that any combination is possible. Whether this is true for grasses is unknown. In this paper, the subfamily Panicoideae s.s. (panicoid grasses) was chosen as the model group to investigate this aspect of grass inflorescence evolution. We have studied the inflorescence morphology of 201 species to complement information available in the literature. We have identified the most recurrent inflorescence types and character states among panicoids. Using multivariate approaches, we have indentified correlations among different inflorescence character states. By phylogenetic reconstruction methods we have inferred the patterns of panicoid inflorescence evolution. Our results demonstrate that not all theoretical combinatorial patterns of variation are found in panicoids. The fact that each panicoid lineage has a unique pattern of inflorescence evolution adds an evolutionary component to combinatorial model.","palabras_clave":"Inflorescence|Morphology|Evolution|Panicoideae|Grass family"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63963,"idioma_id":4,"titulo":"Abundance and Spatial Distribution of Commerson's Dolphin (Cephalorhynchus commersonii) at a breeding site: Ría Deseado, Patagonia, Argentina","resumen":"Abundance and spatial distribution of the Commerson?s dolphin (Cephalorhynchus commersonii) during spring and summer 2002-2004 and fall\/winter 2003 were estimated in the estu-ary Ría Deseado, Patagonia, Argentina. To estimate the abundance, photo-identification and mark-recapture (positive method) were combined. Distribution of dolphins within the estuary was studied, and their locations were recorded using a GPS to perform a Kernel Density Analysis. Adult abundance was higher during spring (34 and 35 individuals in 2002 and 2003, respectively) and decreased during fall (7 individuals) and winter (16 individuals). Sightings of 132 adults during winter, spring, and summer (n = 12, n = 76, and n = 44, respectively) and 14 calves during spring and summer (n = 4 and n = 10, respectively) were recorded; mean school size was 2.0 ± 1.2. Distribution and abundance varied seasonally. During spring, dolphins were more equally dis-tributed through the estuary than during summer when calves and adults remained mainly inside the estuary. The presence of dolphins year-round suggested that the inhabitants are residents of the estuary. This study offers new information on the ecology of the Commerson?s dolphin and confirms Ría Deseado as a breeding site for this species.","palabras_clave":"COMERSON DOLPHIN|CEPHALORHYNCHUS COMERSONII|MARK-RECAPTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63964,"idioma_id":5,"titulo":"Empatía: Desde la Percepción Automática hasta los Procesos Controlados","resumen":"Desde la primera utilización del término Empatía en Psicología a principios del siglo XX su uso se ha extendido por las distintas ramas de esta ciencia, dando lugar a la proliferación de teorías y categorías concebidas para explicar su funcionamiento o describir sus componentes. En el presente artículo revisamos algunas discusiones sobre los alcances y los límites del concepto de empatía, intentando aclarar las particularidades y las relaciones entre términos que conviven en la investigación sobre el tema: Contagio Emocional, Empatía Emocional, Toma de Perspectiva, Teoría de la Mente y Mentalización. Proponemos además una integración de este campo conceptual en un esquema amplio, buscando dar cuenta de la complejidad del constructo de Empatía.","palabras_clave":"EMPATÍA|EMPATÍA EMOCIONAL|TEORÍA DE LA MENTE|MENTALIZACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63966,"idioma_id":5,"titulo":"Explotación faunística en el Sureste de Lípez (Potosí, Bolivia) durante el Período Prehispánico Tardío","resumen":"El Sureste de Lípez (Altiplano boliviano) es una región árida y fría donde no es posible la práctica de la agricultura. Las evidencias arqueológicas, sin embargo, muestran una ocupación continua desde el Período Arcaico. Fuentes coloniales del siglo XVI (Capoche, Lozano Machuca) caracterizan a la población de esta región como dedicada a una combinación de pastoreo, caza, recolección y pesca en lagunas y pantanos, con un patrón de residencia disperso y sumamente móvil. La población actual se especializa en el pastoreo, actividad que se complementa mediante el tráfico caravanero de larga distancia. Prospecciones arqueológicas orientadas a caracterizar aspectos generales de la subsistencia y residencia prehispánicas confirmaron la presencia de un patrón de asentamiento disperso, aparentemente asociado con arquitecturas de material perecedero. Este trabajo presenta los resultados del análisis de conjuntos de arqueofaunas recuperados mediante la excavación de nueve pozos de sondeo en dos sitios Prehispánicos Tardíos (ca. 1200-1600 d.C.). Concluimos que el pastoreo de llamas fue la principal actividad de subsistencia en estos sitios, suplementada con la caza de camélidos silvestres.","palabras_clave":"Altiplano Boliviano|Caza|Pastoreo|Estrategias"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63967,"idioma_id":4,"titulo":"Improved Chemical Control of Chagas Disease Vectors in the Dry Chaco Region","resumen":"The effectiveness of two doses of suspension concentrate (SC) pyrethroid insecticides in suppressing peridomestic populations of Triatoma infestans (Klug) was evaluated in 28 rural communities located in Santiago del Estero province, northwestern Argentina, including 388 houses and 1,516 identiÞed sites. Four treatments were randomly assigned to peridomiciles within each community: 5% SC-cypermethrin at standard (S, 50 mg active ingredient [AI]\/m2) and double dose (2S), and 2.5% SC deltamethrin at standard (D, 25 mg [AI]\/m2) and double dose (2D). Simultaneously, we assessed the effects of both pyrethroids applied at standard doses against domestic infestations.Bug infestation at the site level was assessed by timed manual collections with a dislodging agent at baseline, 13 and 21 mo postspraying (MPS). In domiciles, D and S nearly suppressed all T.infestans infestations up to 21 MPS. In peridomestic sites infested before interventions, multiple logisticregression analysis showed that site-level reinfestation at 13 MPS was signiÞcantly lower for treatment2D (1%) than for other treatments, whereas 2S (6%), D (5%), and S (14%) did not differ signiÞcantly between them. The risk of reinfestation after spraying was signiÞcantly greater in goat or pig corrals than in other peridomestic ecotopes (in which treatments did not differ signiÞcantly), and in sites infested before interventions than in uninfested sites. The application of SC deltamethrin at double dose in goat or pig corrals may suppress T. infestans foci and achieve more sustained effects in the dryChaco.","palabras_clave":"vector control|pyrethroids|chaco|chagas disease"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63968,"idioma_id":4,"titulo":"ANTIOXIDANT TREATMENT REVERTS INCREASED ARTERIAL BASAL TONE AND OXIDATIVE STRESS IN NEPHRECTOMIZED (5\/6) HYPERTENSIVE RATS","resumen":"ABSTRACT  Non-ischemic 5\/6 nephrectomized rat (NefR) is a model of chronic kidney disease.  However, little is known about vascular dysfunction and its relation with  hypertension in NefR. Aims: To evaluate possible alterations of endothelial  function, NO-bioability and basal tone in aorta from NefR and the role of oxidative  stress.  Sprague Dawley rats were divided in: sham rats (SR), NefR and NefR treated with  tempol (NefR-T). Mean arterial pressure (MAP) and renal function was determined.  In isolated aortic rings was measured: 1-Endothelial function, 2-Basal tone, 3-NO  levels, 4-Membrane potential (MP) and 5-Oxidative stress.  NefR increased MAP (SR: 119±4 mmHg; n=7; NefR 169±6; n=8; p<0.001). Tempol  did not modified MAP (NefR-T 168±10; n=6; p<0.001). NefR showed endothelial  dysfunction, increased basal tone and decreased NO levels (SR: 32±2 nA; n=7,  NefR: 10±2; n=8; p<0.001). In both in vitro and in vivo tempol improve basal tone,  NO levels and MP. Oxidative stress in NefR was reverted in NefR-T.  We described, for the first time, that aorta from NefR presented increased basal  tone related to endothelial dysfunction and decreased NO-bioability. The fact that  tempol improves NO-contents and basal tone, without decrease MAP, indicates  that oxidative stress could be implicated early and independently to hypertension,  in the vascular alterations.","palabras_clave":"OXIDATIVE STRESS|NITRIC OXIDE|HYPERTENSION|ANTIOXIDANT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63969,"idioma_id":4,"titulo":"Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation","resumen":"The use of native microorganisms is a useful strategy for phenol bioremediation. In the present work, a bacterial strain, named RTE1.4, was isolated from effluents of a chemical industry. The strain was able to grow at high concentrations of phenol and its derivatives, such as guaiacol, 2,4-dichlorophenol and pentachlorophenol, as well as in a medium containing industrial effluents. This bacterium was identified as Acinetobacter sp. using morphological, physiological, biochemical and 16S rRNA gene analysis. Acinetobacter sp. RTE1.4 degraded phenol (200 to 600 mg\/L) at wide pH range and temperature (5?9 and  25?37&#9702;C, respectively) demonstrating high adaptation ability to different conditions. The strain would metabolize phenol by the ortho-pathway since catechol 1,2-dioxygenase activity was detected. When bacteria were grown in medium containing phenol, an altered whole-cell protein pattern was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with the lack of some low-molecular mass polypeptides and an increase in the relative abundance of high molecular mass proteins after treatment. Considering that the use of native strains in bioremediation studies shows several ecological advantages and that the studied bacterium showed high tolerance and biodegradation  apabilities, Acinetobacter sp. RTE1.4 could be an appropriate microorganism for improving bioremediation and biotreatment of areas polluted with phenol and\/or some of its derivatives. Moreover, the establishment of the optimal growth conditions (pH, temperature, concentration of the pollutant) would provide baseline data for bulk production of the strain and its use in bioremediation processes.","palabras_clave":"BIOREMEDIATION|PHENOL|EFFLUENTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63970,"idioma_id":4,"titulo":"Restricted Outbreak of American Tegumentary Leishmaniasis with High Microfocal Transmission","resumen":"Cutaneous leishmaniasis is endemic in Salta, the northwestern province of Argentina. We describe an outbreak involving five recreational hunters whose exposure was limited to several hours in a residual patch of primary forest. All patients presented with typical cutaneous lesions after a mean incubation period of 59 days (range 15-78), and one developed simultaneous mucosal involvement. Polymerase chain reaction analysis of lesions confirmed Leishmania (V.) braziliensis as the etiologic agent in three cases. All patients were cured with anti-Leishmania treatment. Entomologic surveys in the transmission area revealed a predominance of Lutzomyia neivai. This outbreak report confirms a microfocal transmission pattern of tegumentary leishmaniasis in the Americas and based on a well-determined exposure, allows the determination of incubation times for leishmaniasis caused by Leishmania braziliensis.","palabras_clave":"ADULT|AMPHOTERICIN B|ANIMALS|ANTIPROTOZOAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63971,"idioma_id":4,"titulo":"Selecting salt-tolerant clones and evaluating genetic variability to obtain parents of new diploid and tetraploid germplasm in rhodesgrass (Chloris gayana K.)","resumen":"We evaluated survival percentage under salt stress in 46 diploid and tetraploid clones of rhodesgrass (Chloris gayana K.) with the aim of obtaining salt tolerant clones. Fifteen clones were selected at 600 mM NaCl under hydroponic conditions. Survival percentage of the selected clones ranged between 50\u2013100% and 50\u201375% for diploid and tetraploid clones, respectively. Genetic diversity among the 15 salt-tolerant clones was assessed using amplified fragment length polymorphism (AFLP). All tetraploid clones showed genetic diversity, whereas the diploid group included some genetically related clones. Clones tolerant at 600 mM NaCl and showed genetic diversity are proposed as parents for new synthetic varieties of each rhodesgrass ploidy.","palabras_clave":"AFLP|CHLORIS GAYANA K.|GENETIC IMPROVEMENT|SALINITY TOLERANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63972,"idioma_id":5,"titulo":"El tratamiento en varones sintomáticos con hiperplasia suprarrenal no clásica (HSCNC) podría mejorar la talla adulta","resumen":"La hiperplasia suprarrenal congénita no clásica puede ser asintomáticao presentar signos de hiperandrogenismo y requerirtratamiento. Se describen las características clínicas en el diagnóstico,tratamiento y seguimiento hasta alcanzar la talla adultaen cuatro varones. La edad en el momento del diagnósticofue de 9,2 a 11,6 años. Los motivos de consulta fueron pubarcaprecoz (n= 2), edad ósea acelerada (n= 1) y pubertad precoz(n= 1). Todos los pacientes presentaron 17-hidroxiprogesteronaelevada y el estudio molecular confirmó el diagnóstico. Laedad ósea adelantada respecto de la edad cronológica (13,1 ± 0,5contra 10,2 ± 1,1; p = 0,008) motivó el inicio del tratamientocon hidrocortisona. Durante el seguimiento, la media de talladisminuyó 1,4 ± 0,4 desviaciones estándar (DE) con respecto aldiagnóstico (p= 0,007). Sin embargo, la media de talla final nodifirió de la genética (-0,9 ± 0,7 contra -0,04 ± 0,5 DE; p= 0,054).Conclusión: El tratamiento de los cuatro niños con adelantode la edad ósea en el momento del diagnóstico permitió lograruna talla adulta que no difirió de la talla genética.","palabras_clave":"hiperplasia suprarrenal congénita |tratamiento corticoideo|pubertad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63973,"idioma_id":4,"titulo":"Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat","resumen":"Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost understudied in non-neuronal tissues, though previous studies on germline development during oogenesis may suggest a special function of this protein also in ovarian tissue. In addition, the well-documented association of FMR1 premutation state with fragile X-related premature ovarian insufficiency adds interest to the role of FMRP in ovarian physiology. The aim of the present work was to investigate the expression of Fmr1 mRNA and its protein, FMRP, at different stages of rat follicular development. By immunohistochemical studies we demonstrated FMRP expression in granulosa, theca and germ cells in all stages of follicular development. In addition, changes in Fmr1 expression, both at the protein and mRNA levels, were observed. FMRP levels increased upon follicular development while preantral and early antral follicles presented similar levels of Fmr1 transcripts with decreased expression in preovulatory follicles. These observations suggest that Fmr1 expression in the ovary is regulated at different and perhaps independent levels. In addition, our results show expression of at least four different isoforms of FMRP during all stages of follicular growth with expression patterns that differ from those observed in brain and testis. Our study shows a regulated expression of Fmr1, both at mRNA and protein levels, during rat follicular development. © 2013 Society for Reproduction and Fertility.","palabras_clave":"FMR1|FOLICULOGENESIS|FRAGIL X|OVARY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63974,"idioma_id":4,"titulo":"Dynamics of micro-air-vehicles with flapping wings: A multibody system approach","resumen":"This  paper presents the development of a dynamic model to study the flight mechanics  of a micro-air-vehicle with flapping wings. This model is based on Lagrange?s  equations for constrained systems. The micro-air-vehicle is modeled as a collection  of three rigid bodies (a central body and two wings). The wings have prescribed  motions relative to the central body, i.e., they are kinematically driven. The  numerical integration of all the governing equations, which are  differential-algebraic, is performed simultaneously and interactively in the  time domain.              The integration scheme  couples a 4th-order predictor-corrector method, the modified method  of Hamming, with a procedure to stabilize the resulting  differential-algebraic equations.","palabras_clave":"Multibody system|MAV|flapping wings"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63975,"idioma_id":4,"titulo":"Isolated p.h62l mutation in the cyp21a2 gene in a simple virilizing 21- hydroxylase deficient patient","resumen":"Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency accounts for 90?95% of CAH cases. This autosomal recessive disorder, has a broad spectrum of clinicalforms, ranging from severe or classical, which includes the salt-wasting and simplevirilising forms, to the mild late onset or non-classical form. Most of the disease-causingmutations described are likely to be the consequence of non-homologous recombination orgene conversion events between the active CYP21A2 gene and its homologous CYP21A1Ppseudogene. Nevertheless, an increasing number of naturally occurring mutations havebeen found in disease-causing alleles in the last years.The change p.H62L is one of the most frequent rare mutations of the CYP21A2 gene. Todate, thirty-one 21-hydoxylase-deficient patients have been reported carrying the pH62Lmutation. It was suggested that the p.H62L represents a mild mutation that may beresponsible of a more severe enzymatic impairment when presented with another mildmutation on the same allele.In this report, it is described a 20-year-old woman carrying an isolated p.H62L mutation incompound heterocygozity with c.283-13A\/C>G mutation. Although a mildly non-classicalphenotype was expected, clinical signs and hormonal profile of the patient is consistentwith a more severe simple virilizing form of 21-hydroxylase deficiency.The study of genotype-phenotype correlation in additional patients presenting this mutationwould help in defining the role of this variable in disease manifestation","palabras_clave":"Congenital Adrenal Hyperplasia|21-hydroxylase deficiency|Isolated pH62L mutation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63976,"idioma_id":4,"titulo":"Combined VEGF gene transfer and erythropoietin in ovine reperfused myocardial infarction","resumen":"Background: In reperfused acute myocardial infarction (RAMI), cardioprotective treatments may enhance myocardial salvage and hence reduce the area of necrosis. Based on studies showing that plasmid-mediated vascular endothelial growth factor (pVEGF) gene transfer reduces infarct size by combining angio-arteriogenic and cardiomyogenic effects and that erythropoietin (EPO) exerts anti-apoptotic actions in animal models of AMI, we aimed to assess if their association would reduce infarct size to a larger extent than any of them individually in a large mammalian model of RAMI.Methods: Adult sheep subjected to 90-minute coronary artery occlusion received upon reperfusion intramyocardial pVEGF 3.8 mg plus intravenous EPO 1000 IU\/kg (n=8), pVEGF (n=8), EPO (n=8) or  placebo (n=8).Results: Fifteen days after treatment, infarct size was smaller in the 3 treatment groups (pVEGF+EPO: 8±1%;pVEGF: 16±5%; EPO: 13±4%) compared to placebo (25±7%, pb0.001). However, in the EPO+VEGF group infarct size was significantly smaller than in the groups receiving EPO or VEGF individually (pb0.05). DNA fragmentation, a hallmark of late apoptosis, was significantly lower in sheep receiving EPO. The combined  treatment, while not affecting global left ventricular performance, improved regional peri-infarct function and prevented over-time expansion of the post-infarct perfusion defect.Conclusions: Combined pVEGF and EPO treatment might be clinically useful to enhance the benefits of early revascularization in patients with acute myocardial infarction.","palabras_clave":"VEGF|ERYTHROPOIETIN|REPERFUSED MYOCARDIAL INFARCTION|SHEEP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63977,"idioma_id":4,"titulo":"Gyrolayout: A Hyperbolic Level-of-Detail Tree Layout","resumen":"Many large datasets can be represented as hierarchical structures,  introducing not only the necessity of specialized tree visualization techniques, but also the requirements of handling large amounts of data and offering the user a useful insight into them. Many two-dimensional techniques have been developed, but 3-dimensional ones, together with navigational interactions, present a promising appropriate tool to  deal with large trees.  In this paper we present a hyperbolic tree layout extended to support different level-of-detail techniques and suitable for large tree representation and visualization. This layout permits the visualization of large trees with different level of detail in an enclosed 3-dimensional volume. As a significant part of the layout, we also present a Weighted Spherical Centroidal Voronoi Tessellation, an extension of planar Weighted Centroidal Voronoi Tessellations, in order to find an appropriate distribution of nodes on a spherical surface.","palabras_clave":"Hyperbolic Layout|Einstein Gyrovector Space|Centroidal Voronoi Tessellation|Tree Visualization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63978,"idioma_id":5,"titulo":"Comprensión del imaginario colectivo para la protección del patrimonio arqueológico monumental del Sistema Serrano de Ventania","resumen":"Durante los años 2010 y 2011, se llevaron a cabo diversas tareas vinculadas a la protección, difusión e investigación del patrimonio arqueológico monumental del Sistema Serrano de Ventania, en el marco de los proyectos de extensión universitaria acreditados en la Facultad de Ciencias Naturales y Museo de la Universidad Nacional de La Plata y en la Facultad de Humanidades y Artes de la Universidad Nacional de Rosario en colaboración con otras instituciones provinciales (Instituto Cultural de la Provincia de Buenos Aires) y municipales (Coronel Pringles, Saavedra y Tornquist).   A partir del tipo de patrimonio arqueológico de la zona, que incluye estructuras líticas, piedras paradas, cuevas y aleros con arte rupestre y sitios en estratigrafía, se consideró que su alta visibilidad deja a este registro más susceptible de sufrir deterioro por factores antrópicos, por lo cual se diseñaron distintas estrategias de difusión del conocimiento arqueológico, con el fin de prevenirlo. Entre las acciones más relevantes efectuadas, se pueden mencionar el registro del estado de conservación de los sitios arqueológicos con evidencias de monumentalidad, el relevamiento de opinión en las localidades de Tornquist, Saavedra y Pigüé,  los encuentros participativos abiertos a todos los sectores de la población, el dictado de talleres interactivos para escolares entre 10 y 15 años y la realización de la I Feria de Ciencias Arqueológicas, entre otros. A través de las actividades realizadas se propuso la valoración del patrimonio arqueológico como recurso no renovable y la concientización de la comunidad para garantizar la protección del mismo.","palabras_clave":"PATRIMONIO ARQUEOLÓGICO|SISTEMA SERRANO DE VENTANIA|EXTENSIÓN UNIVERSITARIA|RELEVAMIENTO DE OPINIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63979,"idioma_id":5,"titulo":"NUEVOS RELEVAMIENTOS DE ESTRUCTURAS LÍTICAS Y PIEDRAS PARADAS EN LAS NACIENTES DEL RIO SAUCE GRANDE (PARTIDO DE TORNQUIST, PROVINCIA DE BUENOS AIRES)","resumen":"En este trabajo se presentan los hallazgos vinculados a relevamientos de estructuras líticas y piedras paradas realizados en el partido de Tornquist durante el mes de agosto de 2012. El equipo de investigación accedió a diversos predios rurales próximos a la localidad de Villa Ventana, con el fin de prospectar los sitios arqueológicos, en el marco de proyectos de investigación acreditados en las Universidades Nacionales de La Plata y Rosario.","palabras_clave":"ESTRUCTURAS LÍTICAS|PIEDRAS PARADAS|RIO SAUCE GRANDE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63980,"idioma_id":4,"titulo":"Combined effects of cellulose nanocrystals and silver nanoparticles on the barrier and migration properties of PLA nano-biocomposites","resumen":"Poly(lactic acid) (PLA)-based high performance nano-biocomposites were prepared to be used in active food packaging. Pristine (CNC) and surfactant modified cellulose nanocrystals (s-CNC) with silver (Ag)nanoparticles were used as the matrix modifiers. Binary and ternary systems were prepared.Morphological investigations revealed the good distribution of silver nanoparticles in PLA ternary systems.The combination of s-CNC and Ag nanoparticles increased the barrier effect of the produced films while the results of overall migration for the PLA nano-biocomposites revealed that none of the samples exceeded the overall migration limit, since results were well below 60 mg kg1 of simulant.","palabras_clave":"Poly(lactic acid)|Nano-biocomposites|Cellulose nanocrystals |Silver nanoparticles|Barrier properties|Migration kinetics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63981,"idioma_id":4,"titulo":"Binary PVA bio-nanocomposites containing cellulose nanocrystals extracted from different natural sources: Part I","resumen":"PVA bio-nanocomposites reinforced with cellulose nanocrystals (CNC) extracted from commercial microcrystalline cellulose (MCC) and from two types of natural fibres, Phormium tenax and Flax of the Belinka variety, were produced by solvent casting in water. Morphological, thermal, mechanical and transparency properties were studied while the respective efficiency of the extraction process of CNC from the three sources was evaluated. The effect of CNC types and content on PVA properties and water absorption capacity were also evaluated. Natural fibres offered higher levels of extraction efficiency when compared with MCC hydrolysis yield. Thermal analysis proved that CNC promotes the crystallization of the PVA matrix, while improving its plastic response. It was also clarified that all PVA\/CNC systems remain transparent due to CNC dispersion at the nanoscale, while being all saturated after the first 18\u201324&nbsp;h of water absorption.","palabras_clave":"PVA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63982,"idioma_id":4,"titulo":"Analysis of two polyhydroxyalkanoate (PHA) synthases in Bradyrhizobium japonicum USDA 110","resumen":"Bradyrhizobium japonicum USDA 110 has five polyhydroxyalkanoate (PHA) synthases (PhaC) annotated in its genome: bll4360 (phaC1), bll6073 (phaC2), blr3732 (phaC3), blr2885 (phaC4), and bll4548 (phaC5). All these proteins possess the catalytic triad and conserved amino acid residues of polyester synthases, and are distributed into four different PhaC classes. We obtained mutants in each of these paralogs, and analyzed phaC gene expression and PHA production in liquid cultures. Despite the genetic redundancy, only phaC1 and phaC2 were expressed at significant rates, while PHA accumulation in stationary phase cultures was impaired only in &#916;phaC1. Meanwhile, &#916;phaC2 produced more PHA than the wild-type in this condition and surprisingly, phaC3 transcript increased in the &#916;phaC2 background. Consistently, a double mutant &#916;phaC2\/&#916;phaC3 accumulated less PHA than &#916;phaC2. PHA accumulation in nodule bacteroids followed a similar pattern as in liquid cultures, being prevented in &#916;phaC1 and increased in &#916;phaC2 in relation to the wild-type. Therefore we used these mutants, together with a &#916;phaC1\/&#916;phaC2 doublemutant, to study the B. japonicum PHA requirements for survival, competition for&nbsp;nodulation, and plant growth promotion. All mutants, as well as the wild-type, survived for 60 days in a carbon-free medium, regardless of their initial PHA contents. When competing for nodulation against the wild-type in a 1:1 proportion, &#916;phaC1 and &#916;phaC1\/&#916;phaC2occupied only 13-15 % of the nodules, while &#916;phaC2 occupied 81 %, suggesting that thePHA polymer is required for successful competitiveness. However, the bacteroid content of PHA did not affect the shoot dry weight accumulation.","palabras_clave":"phaC|soybean|nodulation|competition"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63983,"idioma_id":4,"titulo":"MAPK Phosphatase-2 (MKP-2) Is Induced by hCG and Plays a Role in the Regulation of CYP11A1 Expression in MA-10 Leydig Cells","resumen":"MAP kinases (MAPKs) such as extracellular regulated kinases (ERK1\/2) are dephosphorylated, and consequently inactivated, by dual specificity phosphatases (MKPs). In Leydig cells, Luteinizing hormone (LH) triggers ERK1\/2 phosphorylation through the action of protein kinase A (PKA). We demonstrate that, in MA-10 Leydig cells, LH receptor (LHR) activation by human chorionic gonadotropin (hCG) upregulates MKP-2, a phosphatase that dephosphorylates ERK1\/2, among other MAPKs. After 2 h, hCG and 8Br-cAMP significantly increased MKP-2 mRNA levels (3-fold), which declined to basal levels after 6 h. MKP-2 protein accumulation exhibited a similar kinetic profile. In cells transiently expressing flag-MKP-2 protein, hCG\/8Br-cAMP stimulation promoted the accumulation of the chimera (2.5-fold after 3 h of stimulation). Pharmacological and biochemical approaches showed that the accumulation of flag-MKP-2 involves a posttranslational modification that increases MKP-2 half life. MKP-2 downregulation by a short hairpin RNA (MKP-2 shRNA) raised the levels of phosphorylated ERK1\/2 reached by 8Br-cAMP stimulation. This effect was evident after 180 min of stimulation, which suggests that MKP-2 downregulates the late phase of cAMP-induced ERK1\/2 activity. Also, MKP-2 shRNA increased the stimulatory effect of 8Br-cAMP on both promoter activity and messenger levels of CYP11A1, which encodes for the steroidogenic enzyme P450scc and is induced by LH\/hCG through PKA and ERK1\/2 activities. Our findings demonstrate, for the first time, that LH\/hCG tightly regulates MKP-2 expression, which modulates the induction of CYP11A1 by 8Br-cAMP. MKP-2 upregulation might control ERK1\/2 activity in a specific temporal frame to modulate the expression of a finite repertory of ERK-dependent genes.","palabras_clave":"CYP450scc|ERK|Leydig|MKP-2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63984,"idioma_id":4,"titulo":"Nutritional and antioxidant properties of Geoffroea decorticans, an Argentinean fruit, and derived products (flour, arrope, decoction and hydroalcoholic beverage","resumen":"Geoffroea decorticans, popularly known as ?Chañar,? is widely distributed throughout Northwestern Argentina. Its fruit is consumed in different forms (flour, arrope, and hydroalcoholic beverage) in several Argentinean communities. The aim of this work is to quantify macronutrients and phytochemicals of G. decorticans fruit and products derived from it as well as to determine its functional properties and toxicity. A high content of fiber and other phytochemicals (carotenoids, ascorbic acid and polyphenols, principally non flavonoids) were detected in Chañar flour. Due to physicochemical changes may occur during processing fruits that affect the functional properties, different traditional food were obtained from them and its properties were studied. The phenolic compounds content in different arrope types (with and without sugar added) was lower (between 3 and 10 fold) than the Chañar flour. Condensed tannins were the dominant phenolics in the flour but in arrope without sugar decreased 21 fold and in arrope with sugar were not detected. The content of ascorbic acid in arrope was similar to flour. All preparations obtained from flour and arrope showed hydrogen or electron donor capacity and were active as inhibitor of lipoperoxidation. On the other hand, extracts did not show any mutagenic effect. Chañar flour, beverage, decoction and arrope consumption could be encouraged for its functional properties and nutritional properties. The explotation of this native natural resource could be very important for regional economy.","palabras_clave":"chañar|arrope|nutritonal properties|antioxidant"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63985,"idioma_id":4,"titulo":"Nutritional, Antioxidant and Anti-Inflammatory Properties of Cyclanthera pedata, an Andinean Fruit and Products Derived from Them","resumen":"There is an increasing interest in food plant with health-giving effects. The aim of this research was to evaluate the phytochemical and nutraceutical profiles of Cyclanthera pedata fruits, a native fruit of the Andean region used by Incas communities called achojcha. Soluble protein (SP), total sugar (TS), free phenolic compounds (FPC), ascorbic acid (AA), total monomeric anthocyanin (TMA), condensed and hydrolizable tannin (CT, HT) were evaluated by sprectro- photometric methods in fresh fruits with and without heating and in flour obtained from fruits. Multielemental composi- tion by ICP-MS was done. Antioxidant activity (AOA) was determined by ABTS and Linoleic acid&#61538; -carotene meth- ods. The inhibitory capacity of LOX, a pro-inflammatory enzyme was also analyzed. The achojcha fresh fruits and achojcha flour showed low amount calories. The flour was high in potassium (7400 mg·K\/100 g) and low in sodium (77 mg Na\/100 g). High levels of FPC (670 mg GAE\/100 g) were found together with elevated levels of AA (123 mg AA\/ 100 g). The TMA (0.6 mg C3-GE\/100 g) as well as HT (3.4 mg PB2E\/100 g) were also detected in flour. All prepa- rations obtained with fresh and dried fruits showed AOA with SC50 values between 1.8 to 14.5 &#956;g GAE\/mL. Inhibitory capacity on LOX was also demonstrated (IC50 values of 40 µg GAE\/mL). The fresh fruits and flour showed nutraceuti- cal characteristics that are demanded by functional food and could be used as dietary supplement","palabras_clave":"achojcha Fruit| achojcha Flour| Cyclanthera pedata|Nutritional Properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63986,"idioma_id":4,"titulo":"Evaluation of an efficient approach for target tracking from acoustic imagery for the perception system of an autonomous underwater vehicle","resumen":"This article describes the core algorithms of the perception system to be included within an autonomous underwater vehicle (AUV). This perception system is based on the acoustic data acquired from a side scan sonar (SSS).  These data should be processed in an efficient time, so as to the perception system could detect and recognize a predefined target. This detection and recognition outcome is then an important piece of knowledge for the AUV?s dynamic mission planner (DMP). Effectively, the DMP should propose different trajectories, navigation depths and other parameters that will change the robot?s behavior, according to the perception system output. Hence, the time to make a decision is critical to assure safe robot operation and to acquire good quality data, and consequently the efficiency of the on-line image processing from acoustic data, is a key issue. Current techniques for acoustic data processing are time and computationally intensive. Hence, it was decided to process data coming from a SSS using a technique that is used for radars, due to its efficiency and its amenability to on-line processing. The engineering problem to solve in this case, is underwater pipeline tracking for routinely inspections in the off-shore industry. Then, an automatic oil pipeline detection system was developed borrowing techniques from processing of radar measurements. The radar technique is known as Cell Average ? Constant False Alarm Rate (CA ? CFAR). With a slight variation of the algorithms underlying this radar technique, consisting of the previous accumulation of partial sums, a great improvement in computing time and effort is achieved. Finally, a comparison with previous approaches over images acquired with a SSS from a vessel in the Salvador de Bahia bay in Brazil, showed the feasibility of using this on-board technique for AUV perception.","palabras_clave":"CA-CFAR|SIDE-SCAN SONAR|AUTOMATIC OBJETIVE DETECTION|SONAR IMAGERY|AUV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63988,"idioma_id":4,"titulo":"Nitric oxide participates at the first steps of Apis mellifera cellular immune activation in response to non-self recognition","resumen":"The honey bee Apis mellifera (Hymenoptera) is being affected by many diseases. The elimination of organisms in the insect hemocoel requires hemocytes recognition and response to the invader. After recognizing a surface as ?foreign,? hemocytes ?spread.? Spreading on glass surfaces by insect hemocytes is used as a measure of immune activation. Nitric oxide (NO) is a signaling and immune effector molecule in response to microbial infection that has been proposed as a key molecule in invertebrate immunity. The participation of NO in the hemocytic response of A. mellifera upon recognition of non-self is herein analyzed. Glass-adherent hemocytes produce large amounts of NO. Treatment with NO donor sodium nitroprusside enhanced hemocyte spreading, while NO scavenger carboxyPTIO reduced hemocyte immune activation. These results are indicative of NO participation at the beginning of A. mellifera immune response to non-self.","palabras_clave":"nitric oxide|Apis mellifera|immune response"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63989,"idioma_id":4,"titulo":"Differentiation of triticale cultivars through FISH karyotyping of their rye chromosomes","resumen":"The aim of this work was to characterize triticale cultivars cytogenetically through In situ hybridization (FISH) analysis of their rye chromosomes. In the present work we studied six cultivars of triticale (?Cayú-UNRC?, ?Cumé-UNRC?, ?Genú-UNRC?, ?Ñinca-UNRC?, ?Quiñé-UNRC? and ?Tizné-UNRC?), released by the Universidad Nacional de Río Cuarto (UNRC), Córdoba, Argentina. All of them were obtained from the CIMMYT and improved for fresh forage, haymaking and feed grain at the UNRC. The distribution and organization of highly repetitive DNA sequences of Secale cereale (pSc74, pSc200, pSc250 and pSc119.2) using FISH analyses, revealed a specific localization of the signals for several rye chromosomes, which allowed us to distinguish the cultivars. Cluster analysis showed a great cytogenetic similarity among the rye cultivars used to originate these hybrids. The knowledge of the variability among triticale cultivars is necessary to propose futures crosses in breeding programs. This study will also be valuable to identify commercial seeds and to analyze the possible association between agronomic characters and the presence of certain rye chromosomes or specific regions in these chromosomes.","palabras_clave":"CYTOGENETIC SIMILARITY|FISH|PSC74|PSC119.2|PSC200|PSC250"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63990,"idioma_id":4,"titulo":"Implications of the kinematical structure of circumnuclear star-forming regions on their derived properties","resumen":"We review the results of high dispersion spectroscopy of 17 circumnuclear starforming regions (CNSFRs) in 3 nearby early spiral galaxies, NGC 2903, NGC 3310 and  NGC 3351.  We &#64257;nd that single Gaussian &#64257;tting to the H&#946; and [Oiii]&#955;5007&#730;A line pro&#64257;les  results in velocity dispersions around 32 km s&#8722;1 and 52 km s&#8722;1  , respectively, while the  IR Caii triplet cross-correlation technique provides stellar velocity dispersion values  close to 50 km s&#8722;1  .  Even though multiple kinematical components are present, the relation between  gas velocity dispersion and Balmer emission line luminosity (L-&#963; relation) reproduces  the correlation for disk giant Hii regions albeit with a larger scatter. The scatter in  the L-&#963; relation is considerably reduced when theoretical evolutionary corrections are  applied suggesting that an age range is present in the sample of CNSFRs.  To analyse the observed complex pro&#64257;les, we performed multiple Gaussian component &#64257;ts to the H&#946; and [Oiii]&#955;5007&#730;A lines obtaining optimal &#64257;ts with two Gaussians  of di&#64256;erent width. These best &#64257;ts indicate that the narrower component has average velocity dispersion close to 23 km s&#8722;1 while the broader component shows average  values in the range 50-60 km s&#8722;1  for both lines, close to the observed stellar velocity  dispersions. The &#64258;uxes of the broad and narrow H&#946; components are similar. This is  not the case for [Oiii]&#955;5007&#730;A for which the broad components have higher &#64258;uxes than  the narrow ones, thus producing a clear segregation in their [Oiii]\/H&#946; ratios.  We suggest a possible scenario for understanding the behaviour of CNSFRs in the  L-&#963; and &#963;gas-&#963;&#8727; diagrams involving an inner gaseous disk responsible for the narrow  component of the emission lines.  Our main conclusion is that the presence of di&#64256;erent kinematical components with  similar total &#64258;uxes in the emission line spectrum of CNSFRs raises important doubts  regarding properties of the ionized gas derived from global line ratios obtained with  low resolution spectroscopy in star-forming regions in the central regions of early type  galaxies. Given the ubiquity of these star-forming systems, ionized gas analyses should  be done preferably from high dispersion spectra with high spatial resolution.","palabras_clave":"HII regions|galaxies: kinematics and dynamics|galaxies: starburst|galaxies: star clusters"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63991,"idioma_id":4,"titulo":"Optical and mid-infrared neon abundance determinations in star-forming regions","resumen":"We have used observational spectroscopic data of star-forming regions compiled from the literature and photoionization models to analyse the neon ionic abundances obtained using both optical and mid-infrared emission lines. Comparing Ne++\/H+ ionic abundances from distinct methods, we have found that, on average, the abundances obtained via infrared emission lines are higher than those obtained via optical lines, by a factor of 4. Photoionization models with abundance variations along the radius of the hypothetical nebula provide a possible explanation for a large part of the difference between ionic abundances via optical and infrared emission lines. The ionization correction factor (ICF) for the neon is obtained from direct determinations of ionic fractions using infrared emission lines. We derive a constant Ne\/O ratio (log Ne\/O &#8776; -0.70) for a large range of metallicities, independently of the ICF used to compute the neon total abundance.","palabras_clave":"galaxies: abundances|galaxies: evolution|galaxies: formation|galaxies: general|galaxies: ISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63992,"idioma_id":4,"titulo":"Does the relationship between prenatal care and birth weight vary by oral clefts? evidence using South American and United States samples","resumen":"Does the Relationship between Prenatal Care and Birth Weight Vary by Oral Clefts? Evidence Using South American and United States Samples.Nyarko KA, Lopez-Camelo J, Castilla EE, Wehby GL.SourceDepartment of Health Management and Policy, University of Iowa, Iowa City, IA.AbstractOBJECTIVE: To evaluate if the association between prenatal care use and birth weight (BW) varies for infants with&nbsp;cleft lip and\/or cleft palate (CL\/P), classified into isolated and non-isolated forms, compared with unaffected infants.STUDY DESIGN: The study employed 2 datasets. The first included a multi-country sample of 2405 infants with CL\/P and 24&#8200;046 infants without CL\/P born in 1996-2007 in South America. The second was a sample of 2122 infants with CL\/P and 297&#8200;415 without CL\/P from the United States 2004 natality dataset. Separate analyses were performed for the South American and United States samples. The association between prenatal care and BW was evaluated separately for isolated CL\/P, non-isolated CL\/P, and unaffected infants using regression models adjusting for several background characteristics.RESULTS: Prenatal care was associated with improved BW for all infant groups, with greater BW increases for infants with CL\/P particularly non-isolated forms. In the South American sample, BW increased by 108, 69, and 40 g on average per prenatal visit for infants with non-isolated CL\/P, infants with isolated CL\/P, and unaffected infants, respectively. In the United States sample, BW increased by 51, 21, and 16 g on average per prenatal visit for these infant groups, respectively.CONCLUSIONS: Prenatal care was associated with larger BW increases for pregnancies complicated with CL\/P, particularly non-isolated forms, compared with unaffected pregnancies. Given that reduced BW is a well-recognized comorbidity of CL\/P, the findings highlight the importance of prenatal care for at-risk pregnancies as a tertiary-prevention intervention to reduce the health burden of CL\/P.Copyright © 2013 Mosby, Inc. All rights reserved.","palabras_clave":"Birth weight, |CL\/P, |Cleft lip and\/or cleft palate, |ECLAMC, Latin American Collaborative Study of Congenital Malformations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63993,"idioma_id":4,"titulo":"Explaining racial disparities in infant health in Brazil","resumen":"OBJECTIVES: We sought to quantify how socioeconomic, health care, demographic, and geographic effects explain racial disparities in low birth weight (LBW) and preterm birth (PTB) rates in Brazil.METHODS: We employed a sample of 8949 infants born between 1995 and 2009 in 15 cities and 7 provinces in Brazil. We focused on disparities in LBW (&lt; 2500 g) and PTB (&lt; 37 gestational weeks) prevalence between infants of African ancestry alone or African mixed with other ancestries, and European ancestry alone. We used a decomposition model to quantify the contributions of conceptually relevant factors to these disparities.RESULTS: The model explained 45% to 94% of LBW and 64% to 94% of PTB disparities between the African ancestry groups and European ancestry. Differences in prenatal care use and geographic location were the most important contributors, followed by socioeconomic differences. The model explained the majority of the disparities for mixed African ancestry and part of the disparity for African ancestry alone.CONCLUSIONS: Public policies to improve children's health should target prenatal care and geographic location differences to reduce health disparities between infants of African and European ancestries in Brazil.","palabras_clave":"racial disparities|Brazil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63994,"idioma_id":4,"titulo":"Preferential associated anomalies in 818 cases of microtia in South america","resumen":"The etiology of microtia remains unknown in most cases. The identification of patterns of associated anomalies (i.e., other anomalies that occur with a given congenital anomaly in a higher than expected frequency), is a methodology that has been used for research into the etiology of birth defects. We conducted a study based on cases of microtia that were diagnosed from more than 5 million live (LB)- and stillbirths (SB) examined in hospitals participating in ECLAMC (Latin American Collaborative Study of Congenital Malformations) between 1967 and 2009. We identified 818 LB and SB with microtia and at least one additional non-related major congenital anomaly (cases) and 15,969 LB and SB with two or more unrelated major congenital anomalies except microtia (controls). A logistic regression analysis was performed to identify the congenital anomalies preferentially associated with microtia. Preferential associations were observed for 10 congenital anomalies, most of them in the craniofacial region, including facial asymmetry, choanal atresia, and eyelid colobomata. The analysis by type of microtia showed that for anomalies such as cleft lip and palate, macrostomia, and limb reduction defects, the frequency increased with the severity of the microtia. In contrast, for other anomalies the frequency tended to be the same across all types of microtia. Based on these results we will integrate data on the developmental pathways related to preferentially associated congenital anomalies for future studies investigating the etiology of microtia.","palabras_clave":"anomalies|microtia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63995,"idioma_id":4,"titulo":"Role of TRAV locus in low caries experience","resumen":"Caries is the most common chronic, multifactorial disease in the world   today; and little is still known about the genetic factors influencing   susceptibility. Our previous genome-wide linkage scan has identified   five loci related to caries susceptibility: 5q13.3, 13q31.1, 14q11.2,   14q 24.3, and Xq27. In the present study, we fine mapped the 14q11.2   locus to identify genetic contributors to caries susceptibility. Four   hundred seventy-seven subjects from 72 pedigrees with similar cultural   and behavioral habits and limited access to dental care living in the   Philippines were studied. An additional 387 DNA samples from unrelated   individuals were used to determine allele frequencies. For replication   purposes, a total of 1,446 independent subjects from four different   populations were analyzed based on their caries experience (low versus   high). Forty-eight markers in 14q11.2 were genotyped using TaqMan   chemistry. Transmission disequilibrium test was used to detect over   transmission of alleles in the Filipino families, and Chi-square,   Fisher´s exact and logistic regression were used to test for association   between low caries experience and variant alleles in the replication   data sets. We finally assessed the mRNA expression of TRAV4 in the   saliva of 143 study subjects. In the Filipino families, statistically   significant associations were found between low caries experience and   markers in TRAV4. We were able to replicate these results in the   populations studied that were characteristically from underserved areas.   Direct sequencing of 22 subjects carrying the associated alleles   detects one missense mutation (Y30R) that is predicted to be probably   damaging. Finally, we observed higher expression in children and   teenagers with low caries experience, correlating with specific alleles   in TRAV4. Our results suggest that TRAV4 may have a role in protecting   against caries.","palabras_clave":"TRAV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63996,"idioma_id":4,"titulo":"Effect of age and diet composition on activity of pancreatic enzymes in birds","resumen":"In birds, digestive enzymes produced by pancreas and intestinal epithelium cooperate closely during food hydrolysis. Therefore, activity of pancreatic and intestinal enzymes processing the same substrate can be hypothesized to change together in unison, as well as to be adjusted to concentration of their substrate in the diet. However, our knowledge of ontogenetic and diet-related variation of digestive enzymes in birds is limited mainly to intestinal enzymes, and very little is known if they are accompanied by changes in activity of enzymes produced by pancreas. Here, we analyzed age- and diet-related changes in activities of pancreatic enzymes in five passerine and galloanserinae species, and compared them with simultaneous changes in activity of intestinal enzymes. Mass-specific activity of pancreatic amylase increased with age in young house sparrows but not in zebra finches, in agreement with typical diet starch content and simultaneous changes in activity of intestinal maltase. However, we found little evidence for the presence of adaptive, diet-related modulation of pancreatic enzymes in both passerine and galloanserinae species, even though in several cases the same birds modulated adaptively activities of their intestinal enzymes. In general, diet-related changes in mass-specific activity of pancreatic and intestinal enzymes were usually not correlated. We conclude that activity of pancreatic enzymes in birds is under strong genetic control, that enables evolutionary adjustment to typical diet composition but is less capable for short term, diet-related flexibility.","palabras_clave":"Digestive enzymes|Pancreas|Ontogeny|Developmental flexibility"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63997,"idioma_id":5,"titulo":"Déficit de testosterona y estrés en pulmón","resumen":"Si se pierde el equilibrio entre los sistemas que generan oxidantes y aquellos que generan antioxidantes se produce un estado denominado estrés oxidativo.&nbsp;Los pulmones están expuestos continuamente a agentes oxidantes generados tanto a nivel endógeno, como a nivel exógeno. Se han identificado diferencias sexuales considerables tanto en la fisiología respiratoria, como en la respuesta del pulmón a agentes ambientales. Estas diferencias estarían moduladas en parte por las hormonas sexuales y sus receptores nucleares. La supresión de andrógenos provocaría alteraciones significativas en el pulmón, que pueden estar relacionadas a alteraciones en la composición química de las membranas celulares y del surfactante pulmonar. Estos cambios pueden ser reversibles si se administran andrógenos luego del déficit de testosterona, aunque la reversibilidad depende del tiempo y la duración de la administración posterior de andrógenos. Es interesante explorar el efecto que causa el estrés oxidativo producto de la falta de andrógenos en la fisiología pulmonar y buscar la posibilidad de revertir los síntomas del cuadro inflamatorio que genera.&nbsp;","palabras_clave":"Estrés oxidativo|Pulmón|Castración|Testosterona"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63998,"idioma_id":4,"titulo":"TACO: Efficient SAT-Based Bounded Verification Using Symmetry Breaking and Tight Bounds","resumen":"SAT-based bounded verification of annotated code consists of translating the code together with the annotations to a propositional formula, and analyzing the formula for specification violations using a SAT-solver. If a violation is found, an execution trace exposing the failure is exhibited. Code involving linked data structures with intricate invariants is particularly hard to analyze using these techniques. In this paper, we present Translation of Annotated COde (TACO), a prototype tool which implements a novel, general, and fully automated technique for the SAT-based analysis of JML-annotated Java sequential programs dealing with complex linked data structures. We instrument code analysis with a symmetry-breaking predicate which, on one hand, reduces the size of the search space by ignoring certain classes of isomorphic models and, on the other hand, allows for the parallel, automated computation of tight bounds for Java fields. Experiments show that the translations to propositional formulas require significantly less propositional variables, leading to an improvement of the efficiency of the analysis of orders of magnitude, compared to the noninstrumented SAT-based analysis. We show that in some cases our tool can uncover bugs that cannot be detected by state-of-the-art tools based on SAT-solving, model checking, or SMT-solving.","palabras_clave":"Static Analysis|SAT-based code analysis|Alloy|KodKod|DynAlloy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":63999,"idioma_id":4,"titulo":"Comparison of the kinetics of electron transfer in the diffusion limit for the singlet and triplet quenching of eosin Y by quinones","resumen":"Electron  transfer (ET) rate constants were determined by means of lifetime &nbsp;measurements for the fluorescence quenching  and by laser flash photolysis for the triplet quenching of the dye eosin Y by  p-benzoquinones in acetonitrile. The results represent a new aspect of the  dependence of the rate constants with the driving force in the diffusion limit region.  That is, the rate constants for singlet quenching in the highly negative region  of deltaGet do not decrease as predicted by Marcus theory, but rather show a  small positive dependence on the driving force. However, it is found that, in  the same free energy range, the triplet rate constants are lower than those for  the singlet process. They also increase with the exergonicity of the reaction,  but the dependence with deltaGet is less marked than that found for the singlet  reaction. Even at a Gibbs energy change of -1.0 eV the triplet quenching rate  constants do not reach the theoretical diffusion limit. The results are  analyzed using the current theories for diffusion-mediated ET reactions.","palabras_clave":"electron transfer|diffusion limit|singlet and triplet quenching"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64000,"idioma_id":4,"titulo":"Redescription of Echinocoleus hydrochoeri (Travassos, 1916) (Nematoda: Trichuridae) from Hydrochoeris hydrochaeris Linnaeus, 1766 (Rodentia: Caviidae) from Argentina","resumen":"Twenty eight Capillariinae species were recorded in rodents; one of these was described from a caviomorph rodent, Hydrochoeris hydrochaeris L. 1766, and placed in genus Echinocoleus by Moravec (1982). However, both original description and subsequent contributions of Echinocoleus hydrochoeri are poor and incomplete. Specimens of this species were recovered during a parasitological survey of capybaras from two localities from Corrientes Province, Argentina. In this paper, E. hydrochoeri is redescribed, reviewing its belonging to the genus Echinocoleus. Moreover, a new geographical distribution is reported. The redescription of the E. hydrochoeri is based on morphologic and morphometrical features, such as intestine ending in cloaca beside ejaculatory duct, caudal bursa composed of 2 large ventrolateral lobes, with an fleshy internal part fleshy and an membranous external part membranous (they are not united dorsally with a membrane), terminal part of cylindrical cirrus ornamented with thin and thick spines (and particular pattern distribution), sclerotized spicule sclerotized, one pair of papillae in male, and vulvar appendage in female, and 3 bacillary bands (1 ventral and 2 lateral). The morphological comparison among Echinocoleus species showed that E. hidrochoeri and E. cyanopicae (type species from birds) are the most different within the genus, and that E. hidrochoeri and E. auritae (both mammal parasites), seem be the most similar. However, the paucity of distinctive morphological characteristics from capillariids results in poor descriptions. Consequently, the difficult taxonomic and nomenclatural situations of the capillariid group suggest that E. hidrochoeri may be changed of genus when more species are redescribed including more characters. In this paper, the generic and specific analyses were performed in order to establish new standards for futures studies on the systematic position of Capillariinae species. This survey presents new relevant morphological information and a new record of a capillariid species from Argentina, being the fourth for this country.","palabras_clave":"Capillaridae|Capybara|Echinocoleus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64001,"idioma_id":4,"titulo":"The organophosphate chlorpyrifos disturbs redox balance and triggers antioxidant defense mechanisms in JEG-3 cells","resumen":"Introduction: Reactive oxygen species (ROS) are produced by a number of physiological and pathological processes which influence the function of a diverse array of cellular events. An imbalance between ROS generation and elimination was reported for different environmental xenobiotics exposure. Here, we analyzed the effect of chlorpyrifos (CPF) on the JEG-3 cell antioxidant defense in conditions where cell viability and morphology were not altered.  Methods: Acetylcholinesterase (AChE) activity, reduced glutathione (GSH) content and catalase (CAT) antioxidant enzyme activity were measured by biochemical studies. ROS production was detected using the fluorogenic probe 20,70-dichlorodihydrofluorescein diacetate. The transcript level of superoxide dismutase enzyme 1 (SOD1), glutathione reductase (GR), heme oxygenase-1 (HO-1), and nuclear factor E2-related factor 2 (Nrf2) as well as Nrf2 protein amount were analyzed by quantitative real time PCR and  Western blot, respectively.  Results: The results showed that CPF inhibited AChE activity, induced ROS production, upregulated CAT activity, and decreased GSH concentration. In response to CPF exposure, GR and HO-1 mRNA levels were increased with no changes in SOD1 mRNA. Furthermore, CPF significantly augmented Nrf2 at both mRNA and protein levels trigging the antioxidant status by increasing nuclear Nrf2 translocation.  Discussion and conclusion: Taken together, these data indicate that JEG-3 cells are able to attenuate the oxidative stress induced by CPF through the adaptive activation of the Nrf2\/ARE pathway.","palabras_clave":"chlorpyrifos;|placenta;|antioxidant response;|Nrf2;|JEG-3 cells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64002,"idioma_id":4,"titulo":"P-glycoprotein in sheep liver and small intestine:  gene expression and transport efflux activity","resumen":"The role of the transporter P-glycoprotein (P-gp) in the disposition kinetics of different drugs therapeutically used in veterinary medicine has been demonstrated. Considering the anatomo-physiological features of the ruminant species, the constitutive expression of P-gp (ABCB1) along the sheep gastrointestinal tract was studied. Additionally, the effect of repeated dexamethasone (DEX) administrations on the ABCB1 gene expression in the liver and small intestine was also assessed. The ABCB1 mRNA expression was determined by real-time quantitative PCR. P-gp activity was evaluated in diffusion chambers to determine the efflux of rhodamine 123 (Rho 123) in the ileum from experimental sheep. Theconstitutive ABCB1 expression was 65-fold higher in the liver than in the intestine (ileum). The highest ABCB1 mRNA expression along the small intestine was observed in the ileum (between 6- and 120-fold higher). The treatment with DEX did not elicit a significant effect on the P-gp gene expression levels in any of the investigated gastrointestinal tissues. Consistently, no significant differences were observed in the intestinal secretion of Rho 123, between untreated control and DEX-treated animals. The understanding of the efflux transporters expression and activity along the digestive tract may help to elucidate clinicalimplications emerging from drug interactions in livestock.","palabras_clave":"P-glycoprotein|liver|small intestine|sheep"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64003,"idioma_id":4,"titulo":"Quantification of Acetylated Tubulin","resumen":"The acetylation\/deacetylation of Lys40 of the &#945;-subunit is an important post-translational modification undergone by tubulin during the life of a cell. Many previous studies have addressed the physiological role of this acetylation process using various approaches based on changes of acetylated tubulin (AcTubulin) content. In most of these studies, however, the actual amounts of AcTubulin were not known and it was difficult to draw conclusions. We present here a simple method to estimate the percentage of AcTubulin relative to total tubulin. The method is based on acetylation of the tubulin sample with acetic anhydride, Western blotting stained by anti-AcTubulin antibody, and comparison of the optical density of the AcTubulin band with that of a corresponding sample that was not chemically acetylated.","palabras_clave":"Acetylated tubulin|Quantification method|microtubules|Chemical acetylation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64004,"idioma_id":4,"titulo":"Origins of house reinfestation with Triatoma infestans after insecticide spraying in the Argentine Chaco using wing geometric morphometry","resumen":"Identifying the origins of insect vectors collected after community-wide residual insecticide applications is a relevant challenge in the Gran Chaco region where the main vector of Chagas disease Triatoma infestans usually reinfests human dwellings. Wing geometric morphometry was used to compare the right wings of 63 males and 54 females collected at 4 months post-spraying (MPS) with those from 165 males and 111 females collected before full-coverage spraying with pyrethroids in a well-defined rural area in northeastern Argentina. Male and female wing centroid size resulted significantly larger at 4 MPS than before interventions, but no significant changes in shape were detected. Metric disparity (variance of shape) varied significantly in males but not in females. Using shape variables, a relatively large fraction of post-spraying males (70%) and females (54%) could not be differentiated from those collected at the same source house or at the nearest infested house before interventions. Bugs collected at 4 and 8 MPS in a persistently infested house were mainly assigned to the source house. These results support the hypothesis of persistent bug populations that survived the insecticide application at local spatial scales, and are consistent with the occurrence of vector control failures most likely related to moderate pyrethroid resistance. Wing geometric morphometry is a useful tool for identifying sources of reinfestation, but it is limited by the spatial structure found in the reference populations. Combined with field and genetic data, this approach may contribute to the understanding of the reinfestation process and improvement of vector control strategies.","palabras_clave":"TRIATOMA INFESTANS|GEOMETRIC MORPHOMETRY|REINFESTATION|PYRETHROID INSECTICIDE RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64005,"idioma_id":4,"titulo":"On the description of surface operators in N=2* super Yang-Mills","resumen":"In Ref. [arXiv:1005.4469], Alday and Tachikawa observed that the Nekrasov partition function of N=2 SU(2) superconformal gauge theories in the presence of fundamental surface operators can be associated to conformal blocks of a 2D CFT with affine sl(2) symmetry. This can be interpreted as the insertion of a fundamental surface operator changing the conformal symmetry from the Virasoro symmetry discovered in [arXiv:0906.3219] to the affine Kac-Moody symmetry. A natural question arises as to how such a 2D CFT description can be extended to the case of non-fundamental surface operators. Motivated by this question, we review the results of Refs. [arXiv:0706.1030] and [arXiv:0803.2099] and put them together to suggest a way to address the problem: It follows from this analysis that the expectation value of a non-fundamental surface operator in the SU(2) N=2* super Yang-Mills theory would be in correspondence with the expectation value of a single vertex operator in a two-dimensional CFT with reduced affine symmetry and whose central charge is parameterized by the integer number that labels the type of singularity of the surface operator.","palabras_clave":"Conformal|Field|Quantum|Non-rational"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64006,"idioma_id":4,"titulo":"Structural control on geothermal circulation in the Cerro Tuzgle Tocomar geothermal volcanic area (Puna plateau, Argentina).","resumen":"The reconstruction of the stratigraphical?structural framework and the hydrogeology of geothermal areas is fundamental for understanding the relationships between cap rocks, reservoir and circulation of geothermal &#64258;uids and for planning the exploitation of the &#64257;eld. The Cerro Tuzgle?Tocomar geothermal volcanic area (Puna plateau, Central Andes, NW Argentina) has a high geothermal potential. It is crossed by the active NW?SE trans-Andean tectonic lineament known as the Calama?Olacapato?Toro (COT) fault system, which favours a high secondary permeability testi&#64257;ed by the presence of numerous springs.This study presents new stratigraphic and hydrogeological data on the geothermal &#64257;eld, together with the analysis from remote sensed image analysis of morphostructural evidences associated with the structural framework and active tectonics. Our data suggest that the main geothermal reservoir is located within or below the Pre-Palaeozoic?Ordovicianbasement units, characterised by unevenly distributed secondary permeability. The reservoir is recharged by in&#64257;ltration in the ridges above 4500 m a.s.l., where basement rocks are in outcrop. Below 4500 m a.s.l., the reservoir is covered by the low permeable Miocene?Quaternary units that allow a poor circulation of shallow groundwater. Geothermal &#64258;uids upwell in areas with more intense fracturing, especially where main regional structures, particularly NW?SE COT-parallel lineaments, intersect with secondary structures, such as at the Tocomar &#64257;eld. Away from the main tectonic features, such as at the Cerro Tuzgle &#64257;eld, the less developed network of faults and fractures allows only a moderate upwelling of geothermal &#64258;uids and a mixing between hot and shallow cold waters. The integration of &#64257;eld-based and remote-sensing analyses at the Cerro Tuzgle?Tocomar area proved to be effective in approaching the prospection of remote geothermal &#64257;elds, and in de&#64257;ning the conceptual model for geothermal circulation.","palabras_clave":"TUZGLE|GEOTERMIA|PUNA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64007,"idioma_id":4,"titulo":"T helper type 2 bias and type 17 suppression in primary dengue virus infection in infants and young children","resumen":"Background:The immune response to dengue virus (DENV) primary infection in infants and young children is not well characterized. In Northern Argentina,.90% of the population was DENV-na&#305;¨ve before the 2009 outbreak,allowing evaluation of age-dependent primary responses to infection.  Methods:We conducted a comparative study of the immune response to DENV in 27 infected infants, young chil-dren and their mothers. Lymphocyte T helper (Th) 1, Th2, Th17 and inflammatory responses were assayed in blood  during the 2009 DENV-1 epidemic.  Results:The immune response to DENV-1 was significantly biased to Th2 in infected infants and young children,compared to infants with other febrile illnesses (for IL-4 p,0.001) and to their infected mothers (for IL-4 p,0.01). In addition, IL-17 suppression was observed in the memory response to DENV-1 in infected infants (p,0.01 vs placebo).  Conclusion:Age-related differences in the primary response to DENV, characterized by an immature Th2 polariza-tion and Th17 suppression in infants, should be studied further in order to expand our understanding of the mechanism of dengue pathogenesis.","palabras_clave":"DENGUE VIRUS|INFANT|IMMUNE IMMATURITY|TH2 POLARIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64008,"idioma_id":4,"titulo":"Demographic expansions in South America: Enlightening a complex scenario with genetic and linguistic data","resumen":"Native Americans are characterized by specific and unique patterns of genetic and cultural\/linguistic diversities, and this information has been used to understand patterns of geographic dispersion, and the relationship between these peoples. Particularly interesting are the Tupi and Je speaker dispersions. At present, a large number of individuals speak languages of these two stocks; for instance, Tupi-Guarani is one of the official languages in Paraguay, Bolivia, and the Mercosul economic block. Although the Tupi expansion can be compared in importance to the Bantu migration in Africa, little is known about this event relative to others. Equal and even deeper gaps exist concerning the Je-speakers´ expansion. This study aims to elucidate some aspects of these successful expansions. To meet this purpose, we analyzed Native American mtDNA complete control region from nine different populations and included HVS-I sequences available in the literature, resulting in a total of 1,176 samples investigated. Evolutionary relationships were explored through median-joining networks and genetic\/geographic\/linguistic correlations with Mantel tests and spatial autocorrelation analyses. Both Tupi and Je showed general traces of ancient or more recent fission?fusion processes, but a very different pattern of demographic expansion. Tupi populations displayed a classical isolation-by-distance pattern, while Je groups presented an intricate and nonlinear mode of dispersion. We suggest that the collective memory and other cultural processes could be important factors influencing the fission?fusion events, which likely contributed to the genetic structure, evolution, and dispersion of Native American populations.","palabras_clave":"MITOCHONDRIAL DNA|NATIVE AMERICANS|BRAZIL|AMAZON|CONTROL REGION|HAPLOTYPES|DEMOGRAPHY|PHYLOGEOGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64009,"idioma_id":4,"titulo":"Theoretical investigation of the Diels-Alder reactions of unsaturated boronates","resumen":"The Diels\u2013Alder reactions of simple unsaturated boronates have been investigated using computational methods and the results were compared with those for the analogue dihalo- and dialkylboranes. Our results indicate that the activating effect of the boronate moiety is small. All the studied reactions are concerted normal electron-demand Diels\u2013Alder reactions with asynchronous transition structures and weak [4 + 3] C\u2013B secondary orbital interactions, which explains the low experimental reactivity. Both electronic and steric effects contribute to give the observed low stereo- and regioselectivities.","palabras_clave":"Theoretical investigation |Diels-Alder reaction |Boronates"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64010,"idioma_id":5,"titulo":"PROPUESTA DE PLANIFICACIÓN AMBIENTAL COSTERA DEL SECTOR BAHÍA  DE LOS VIENTOS HASTA COSTA BONITA, PROVINCIA DE BUENOS AIRES","resumen":"El presente estudio se desarrolla a lo largo de 6 km de costa, al Este del Puerto de Quequén, Partido de Necochea, provincia de Buenos Aires. En este sector históricamente se vinculan varios usos del territorio en relación con las actividades económicas dominantes, la portuaria, la navegación, la pesca y las actividades deportivas, recreativas y turísticas.  La recuperación económica iniciada en los últimos años ha impulsado el desarrollo y la expansión de actividades en el sector de playa comprendido entre la Escollera Noreste y Costa Bonita. Los sistemas implicados corresponden a las áreas marina-litoral y litoral-urbana, las cuales están sometidas a múltiples jurisdicciones derivadas de las acciones de las instituciones encargadas de su preservación, desarrollo y administración.   En este aporte se proponen zonificaciones en función de la vocación del territorio para el establecimiento de diversas actividades favoreciendo la radicación de usos compatibles del área costera desde Bahía de Los Vientos hasta Costa Bonita.   Las propuestas de sectorización se basan en el análisis de datos obtenidos de relevamientos sistemáticos efectuados en el lapso 2005-2010 en 10 perfiles. Se consideró el patrón de variación del ancho de playa (desde la línea de bajamar hasta la base del acantilado o espaldón, según correspondiese), del volumen de material sedimentario y de las características granulométricas del sedimento.","palabras_clave":"ZONIFICACION COSTERA|PLANIFICACION|PUERTO DE QUEQUEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64011,"idioma_id":5,"titulo":"Estudios de comportamiento y ecología de lagartijas de los géneros Liolaemus y Phymaturus (Iguania: Liolaemini)","resumen":"RESUMENPresentamos un resumen de investigaciones realizadas en su mayoría durante la última décadapor nuestro grupo, sobre comportamiento y ecología de lagartijas del género Liolaemus yPhymaturus de Argentina. Llevamos a cabo estudios sobre su comunicación, territorialidad,elección de pareja, función de la coloración nupcial en hembras, relación entre dieta y estadoreproductivo, cuidado parental, y filogenia basada en el comportamiento de inmersión en arena.Describimos brevemente cada uno de estos temas y reportamos los resultados más relevantes.Además del valor intrínseco de este tipo de estudios, ellos pueden proveer información valiosaque puede ser utilizada en la elaboración de estrategias de manejo y conservación de especiesanimales, particularmente en este grupo de Iguania en el que muchas especies son endémicasy a menudo poco conocidas.Palabras clave: Lagartijas; Despliegues; Elección de Pareja; Cuidado Parental; Filogenia.","palabras_clave":"LAGARTIJAS|DESPLIEGUES|ELECCIÓN DE PAREJA|CUIDADO PARENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64012,"idioma_id":4,"titulo":"Use of differential water sources by Prosopis flexuosa DC: a dendroecological study","resumen":"In central-western Argentina, there is a pronounced water deficit   gradient, from semiarid climate conditions with 500-mm rainfall\/year              to arid climate conditions with 80-mm rainfall\/year. This   climatic transition, governed by the rainfall gradient, occurs between              the Arid Chaco and Monte phytogeographic regions and is   evidenced by differences in vegetation type, structure, dynamics and              tree growth. In turn, the availability of soil moisture,   particularly access to the water table, modifies water use strategies              by trees along this gradient. We analyzed how water   availability, expressed as differences in accessibility to the water   table,              influences Prosopis flexuosa tree rings along a precipitation gradient. In this manner, we try to interpret the growth of species according to the use              of differential water sources. P. flexuosa showed highly varying growth reactions (tree-ring width and hydraulic anatomic parameters) with climate, depending on the              ecology of the site. Along the Arid Chaco-Monte gradient, the growth of P. flexuosa is more dependent on variations in rainfall in those areas where water depth is greater than root spread. The climate signal              was hidden in those regions where the water table is accessible to the root system.","palabras_clave":"ARID CHACO|DEDROCLIMATOLOGY|MONTE|P. FLEXUOSA|WATER TABLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64013,"idioma_id":4,"titulo":"North Atlantic Oscillation influences on radial growth  of Pinus pinea on the Italian mid-Tyrrhenian coast","resumen":"The North Atlantic Oscillation (NAO) is the most important source of winter atmospheric variability in the northern hemisphere. NAO inversely re&#64258;ects the precipitation regime, which plays a fundamental role in Mediterranean regions, e.g., by recharging the water table. As no attempt has been made thus far to analyze the relationship between NAO variability and tree radial growth in coastal Mediterranean conifers, this paper identi&#64257;es the monthly, winter, annual, and decadal in&#64258;uence of NAO on tree-ring chronologies of six planted Pinus pinea L. populations distributed along the Tyrrhenian coasts of central Italy. Through multidimensional analyses, we identi&#64257;ed tree-ring chronology associations in two main groups. The in&#64258;uence of NAO on the regional chronologies was identi&#64257;ed with correlation functions for the comparison period between 1949 and 2003 at both annual and decadal timescales. Results indicate that winter NAO in&#64258;uence on radial tree growth at annual and decadal timescales may depend on geographical location, site characteristics, and the age structure of tree-ring chronologies. These results contribute to a better understanding of the P. pinea coastal forest ecology and provide evidence of large-scale climatic forcings that in&#64258;uence forest Mediterranean ecosystems.","palabras_clave":"DENDROCLIMATOLOGY|MEDITERRANEO|TREE-RINGS|NAO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64014,"idioma_id":4,"titulo":"Geoid modelling in the area of Fagnano Lake, Tierra del Fuego (Argentina): Insights from mean lake-level observations and reduced gravity data","resumen":"This paper evaluates a combined geoid model in the area of Fagnano Lake located in Tierra del Fuego, Argentina. The model includes GPS\/levelling points, gravity data and GPS buoy observations on the lake. The GPS buoy information allowed to determine a mean lake level (MLL) surface which was used to extend the geoid model to an area with restricted access by land (Del Cogliano et al., 2007). An approach to optimize the selection and distribution of the MLL data is developed in order to use them as input in the Equivalent Source Technique, and to combine them with different types of observations.  Furthermore, the global geopotential model EGM2008 is validated in the remote lake area. This model does not include observed gravity data in this region. Its behaviour is compared to the results of our observations along the levelling lines. Differences of several decimetres are found when EGM2008 geoid undulations are compared to geoid undulations derived from observations in this region. In the areas where EGM2008 relies on real gravimetric observations, differences between model and observations are only of a few centimetres.   The comparison of the EGM2008 model with our observations reveals the effect of missing or not representative gravity information may have on the estimation of geoid undulations in high mountain regions. The data on which EGM2008 is based in the Fagnano Lake area does not result from gravimetric observations on land. Therefore, we consider them as not representative and we show that their effect can be significant in such areas.                ","palabras_clave":"GEOID|MEAN LAKE LEVEL|FAGNANO LAKE|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64015,"idioma_id":4,"titulo":"Mitochondrial susceptibility in a model of paraquat neurotoxicity","resumen":"            Paraquat is a highly toxic herbicide capable  of generating oxidative stress and producing brain damage after chronic  exposure. The aim of this research was to investigate the contribution of  mitochondria to the molecular mechanism of apoptosis in an in vivo experimental model of paraquat neurotoxicity.  Sprague-Dawley adult female rats received paraquat (10 mg\/kg i.p.) or saline once a week  during a month. Paraquat treatment increased cortical and striatal superoxide  anion levels by 45% and 18%, respectively. As a consequence, mitochondrial  aconitase activity was significantly inhibited in cerebral cortex and striatum.  Paraquat  treatment increased cortical and striatal lipid peroxidation levels by 16% and  28% respectively, as compared with control mitochondria Also, cortical and striatal cardiolipin  levels were decreased by 13% and 49%, respectively. Increased Bax and Bak  association to mitochondrial membranes was observed after paraquat treatment in  cerebral cortex and striatum. Also, paraquat induced cytochrome c and AIF release from mitochondria.             These findings support the conclusion that  a weekly dose of paraquat during four weeks induces oxidative damage that activates  mitochondrial pathways associated with molecular mechanisms of cell death. The  release of apoptogenic proteins from mitochondria to cytosol after paraquat  treatment would be the consequence of an alteration in mitochondrial membrane  permeability due to the presence of high superoxide anion levels. Also, our  results suggest that under chronic exposure, striatal mitochondria were more  sensitive to paraquat oxidative damage than cortical mitochondria. Even in the presence  of a high oxidative stress in striatum, equal levels of apoptosis were attained  in both brain areas.  ","palabras_clave":"PARAQUAT|APOPTOSIS|CARDIOLIPIN|SUPEROXIDE ANION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64016,"idioma_id":4,"titulo":"Effects of single and co-inoculation with native phosphate solubilising strain Pantoea sp J49 and the symbiotic nitrogen fixing bacterium Bradyrhizobium sp SEMIA 6144 on peanut (Arachis hypogaea L.) growth","resumen":"In the present study, attempts were made to analyze the effect of co-inoculation with an efficient phosphate solubilising native isolate Pantoea sp J49 and the symbiotic nitrogen fixing Bradyrhizobium sp SEMIA 6144 strain on Arachis hypogaea L. plants growth. Single and coinoculation of peanut plants growing in plastic pots containing soil with low P content were developed. Plants were harvested at R1 and R4 growth stages and were analyzed in different growth parameters. Survival of strain Pantoea sp J49 was analyzed in soil samples and in root tissues. Plants inoculated only with Pantoea sp J49 showed the highest shoot and root weight in both reproductive growth stages evaluated. Plants co-inoculated with this strain and Bradyrhizobium sp SEMIA 6144 showed increase in aerial dry weight at R1 stage. Survival assays demonstrated that Pantoea sp J49 survives not only in the peanut rhizosphere but also inside plant tissues, including nodules formed when it was co-inoculated with Bradyrhizobium sp SEMIA 6144. Results obtained in this study confirm the great potential of the native Pantoea sp J49 isolate in the promotion of peanut plant growth, probably related with its capacity to solubilise phosphate","palabras_clave":"Peanut  |Co-inoculation|Phosphate solubilising bacteria|Plant growth promotion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64017,"idioma_id":4,"titulo":"Synflorescence analysis in South American species of Andropogon section Leptopogon (Andropogoneae, Poaceae): a tool to identify different ploidy levels","resumen":"In southern South America, Andropogon sect. Leptopogon is represented by both diploid and hexaploid species. In order to compare the synflorescence structures and floral arrays in relation to ploidy levels in species of Andropogon, the section Leptopogon was used as model. In this study, the synflorescence structure was typologically characterized. The structural analysis was based on the typology system developed by Troll and Weberling, which has proved useful in describing inflorescences. A comparative analysis of the variations observed in the structure of the synflorescence and a morphometric analysis using principal component analysis were carried out. These results revealed that diploid and hexaploid species present differences mainly in the following parameters: synflorescence length, number of internodes of   the enrichment zone main axis, number of internodes and length of paracladia of the trophotagma, number of long paracladia per unit of inflorescence, length of hairs on articulated rachis internodes, pedicellate spikelet length, pedicellate spikelet width, pedicel width, length of hairs on pedicel, sessile spikelet length, awn length and floral system arrangements. This study provides a useful tool to distinguish between diploid and hexaploid species of Andropogon sect. Leptopogon. We propose naming the group of diploid species from Central and South America as the Andropogon selloanus complex.","palabras_clave":"grass inflorescence structure| multivariate analysis| taxonomy|Andropogon"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64018,"idioma_id":4,"titulo":"A new fossil from the Jurassic of Patagonia reveals the early basicranial evolution and the origins of Crocodyliformes","resumen":"Extant crocodylians have a  limited taxonomic and ecological diversity but they belong to a lineage  (Crocodylomorpha) that includes basal and rather generalized species and a  highly diverse clade, Crocodyliformes. The latter was among the most successful  groups of Mesozoic tetrapods, both in terms of taxonomic and ecological  diversity. Crocodyliforms thrived in terrestrial, semiaquatic, and marine  environments, and their fossil diversity include carnivorous, piscivorous,  insectivorous, and herbivorous species. This remarkable ecological and trophic  diversity is thought only to occur in forms with a completely akinetic skull, characterized  by a functionally integrated and tightly sutured braincase-quadrate-palate  complex. However, the patterns of evolutionary change that led to the highly  modified skull of crocodyliforms and that likely enabled their diversification remain  poorly understood. A new basal crocodylomorph from the Late Jurassic of  Patagonia is described here, Almadasuchus  figarii gen. et sp. nov.The new taxon is known from a well preserved  posterior region of the skull as well as other craniomandibular and postcranial  remains. Almadasuchus figarii differs  from all other crocodylomorphs in the presence of six autapomorphic features, including  the presence of a large lateral notch on the upper temporal bar, an otic shelf  of the squamosal that is wider than long, a deep subtriangular concavity on the  posterolateral surface of the squamosal, and an elongated pneumatopore on the  ventral surface of the quadrate. The inclusion of Almadasuchus in a phylogenetic analysis focused on the origin of Crocodyliformes  places the new taxon as the closest known sister group of Crocodyliformes based  supported by synapomorphic features of the skull (e.g., subtriangular  basisphenoid, absence of basipterygoid process, absence of a sagittal ridge on  the frontal, and a flat anterior skull roof with an ornamented dorsal surface).  New braincase information provided by Almadasuchus  and other crocodylomorphs indicates that most of the modifications on the  posterior region of the skull of Crocodyliformes, including the strongly  sutured braincase, quadrate, and the extensive secondary palate appeared in a  stepwise manner, and predated the evolutionary changes in the snout, jaws, and  dentition. This indicates that the progressively increased rigidity of the  skull provided the structural framework that allowed the great ecological  diversification of crocodyliforms during the course of the Mesozoic. The  phylogenetic pattern of character acquisition inferred for the strongly sutured  (akinetic) skull and the appearance of more diverse feeding behaviours that create  high mechanical loads on the skull, provides another interesting parallel  between the evolution of Mesozoic crocodyliforms and the evolutionary origins  of mammals.","palabras_clave":"Crocodyliformes|Braincase|Evolution|Jurassic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64019,"idioma_id":4,"titulo":"Decrease in cytotoxicity of copper-based intrauterine devices (IUD) pretreated with 6-Mercaptopurine and Pterin as biocompatible corrosion inhibitors","resumen":"The copper intrauterine device (IUD) based itscontraceptive action on the release of cupric ions from acopper wire. Immediately after the insertion, a burst release ofcopper ions occurs, which may be associated to a variety ofside e&#64256;ects. 6-Mercaptopurine (6-MP) and pterin (PT) havebeen proposed as corrosion inhibitors to reduce this harmfulrelease. Pretreatments with 1 × 10&#8722;4M 6-MP and 1 × 10&#8722;4 M PT solutions with 1h and 3h immersion times were tested. Conventional electrochemical techniques, EDX and XPS analysis, and cytotoxicity assays with HeLa cell line were employed to investigate the corrosion behavior and biocompatibility of copper with and without treatments. Results showed that copper samples treated with PT and 6-MP solutions for 3 and 1 h, respectively, are more biocompatible than those without treatment. Besides, the treatment reduces the burst release e&#64256;ect of copper in simulated uterine solutions during the &#64257;rst week after the insertion. It was concluded that PT and 6-MP treatments are promising strategies able to reduce the side e&#64256;ects related to the \u201Cburst release\u201D of copper-based IUD without altering the contraceptive action.","palabras_clave":"intrauterine device|copper, copper ions, pterin|6-mercaptopurine|cytotoxicity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64020,"idioma_id":4,"titulo":"Electrochemical Desorption of Thiolates and Sulfur from Nanoparticle and Planar Platinum Surfaces","resumen":"Thiolate-protected platinum nanoparticles have become promising for applications in heterogeneous catalysis and the fabrication of new materials for hydrogen storage. Once nanoparticles have been synthesized and conveniently grafted onto a particular support, thiol removal might be required before its use. Here, thiolate and sulfur electrodesorption from nanoparticle and planar platinum surfaces are comparatively studied by combining ex-situ X-ray photoelectron spectroscopy (XPS) and electrochemical techniques. We show that alkanethiolates and sulfur adsorbed on Pt surfaces are more stable against reductive desorption than these species on Au substrates. Furthermore, for short-chain thiol-capped platinum nanoparticles we observe complete removal of sulfur-containing species. Hence, these results make this procedure suitable for its use in electrocatalysis. As an example, we demonstrate that 2 nm thiomalic acid-protected platinum nanoparticles markedly improve the performance of a hydrogen storage alloy material, with no additional steps in the preparation of the electrodes","palabras_clave":"platinum nanoparticles|XPS|hydrogen storage|thiomalic acid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64021,"idioma_id":4,"titulo":"The significance of modern diatoms as paleoenvironmental indicators along an altitudinal gradient in the Andean piedmont of central Argentina","resumen":"The objectives of this study were to (a) analyze the distribution pattern of diatom assemblages in freshwater systems from central-western Argentina, (b) investigate the key climatic and limnological factors affecting the species distributions, and (c) evaluate the extent and limitations of diatoms as Quaternary paleoenvironmental indicators in the area. Diatoms were sampled and physical and chemical variables were quantified in a total of 45 lotic and lentic sites. Altitude, annual precipitation, and summer (January) temperature data were obtained from a world climate database. Previously published data from an adjacent region was incorporated into the modern dataset. Environmental variables significantly influencing diatom assemblage composition were identified through partial canonical correspondence analyses (CCAs). In order to assess the degree of analogy between modern and fossil assemblages, the modern analog technique (MAT) was applied. Diatom taxa showed distinct abundance patterns in relation to altitude, with a shift from communities dominated by periphitic and benthic taxa in lower sites to communities dominated by small benthic?tychoplanktonic  taxa in high elevated waterbodies. The pH was the strongest variable explaining the variance within the diatom dataset, followed by log-conductivity and summer air temperature. The comparison of modern diatom assemblages with fossil sequences allowed reconstruction of the evolution of shallow, lentic and vegetated habitats. A good analogy between modern and Pleistocene diatom assemblages was found. The strong relationship between diatoms and climate-driven environmental variables, such as pH, conductivity and summer temperature, enhances the importance of the diatom floras as paleoclimatic indicators in the area and encourages future development of quantitative inference models for the region.","palabras_clave":"DIATOMS|PALEOENVIRONMENTAL RECONSTRUCTION|HOLOCENE|PLEISTOCENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64022,"idioma_id":4,"titulo":"Antibiotic prescriptions in critically-ill patients: A Latin American experience","resumen":"Background:It is widely acknowledged that the presence of infection is an important outcome determinant for intensive care unit (ICU) patients. In fact, antibiotics are one of the most common therapies administered in the ICU settings.Aim:To evaluate the current usage of antibiotics in Latin American ICUs.Subjects and Methods:A one-day p-oint prevalence study to investigate the patterns of antibiotic was undertaken in 72 Latin American (LA) ICUs. Data was analyzed using the Statistix 8 statistical software, version 2.0 (USA). Results were expressed as proportions. When applicable, two tailed hypothesis testing for difference in proportions was used (Proportion Test); a P value of <0.05 was considered significant.Results:Of 704 patients admitted, 359 received antibiotic treatment on the day of the study (51%), of which 167\/359 cases (46.5%) were due to hospital-acquired infections. The most frequent infection reorted was nosocomial pneumonia (74\/359, 21%). Only in 264\/359 patients (73.5%), cultures before starting antibiotic treatment were performed. Thirty-eight percent of the isolated microorganisms were Enterobacteriaceae extended-spectrum &#946;-lactamase-producing, 11% methicillin-resistant Staphylococcus aureus and 10% carbapenems-resistant non-fermentative Gram-negatives. The antibiotics most frequently prescribed were carbapenems (125\/359, 35%), alone or in combination with vancomycin or other antibiotic. There were no significant differences in the ?restricted? antibiotic prescription (carbapenems, vancomycin, piperacillin?tazobactam, broad-spectrum cephalosporins, fluoroquinolones, tigecycline and linezolid) between patients with APACHE II score at the beginning of the antibiotic treatment <15 [83\/114 (72.5%)] and &#8805;15 [179\/245 (73%)] (P = 0.96). Only 29% of the antibiotic treatments were cultured directed (104\/359).Conclusion:Carbapenems (alone or in combination) were the most frequently prescribed antibiotics in LA ICUs. However, the problem of carbapenem resistance in LA requires that physicians improve the use of this class of antibiotics. Our findings show that our web-based method for collection of one-day point prevalence was implemented successfully. However, based on the limitations of the model used, the results of this study must be taken with caution.","palabras_clave":"Antibiotics|Carbapenems|Intensive care unit|Usage"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64023,"idioma_id":4,"titulo":"Bounded Lazy Initialization","resumen":"Tight field bounds have been successfully used in the con- text of bounded-exhaustive bug finding. They allow one to check the correctness of, or find bugs in, code manipulating data structures whose size made this kind of analyses previously infeasible. In this article we address the question of whether tight field bounds can also contribute to a significant speed-up for symbolic execution when using a system such as Symbolic Pathfinder. Specifically, we propose to change Symbolic Pathfinder\u2019s lazy initialization mechanism to take advantage of tight field bounds. While a straightforward approach that takes into account tight field bounds works well for small scopes, the lack of symmetry-breaking significantly affects its performance. We then introduce a new technique that generates only non-isomorphic structures and consequently is able to consider fewer structures and to execute faster than lazy initialization.","palabras_clave":"Symbolic Execution|Lazy Initialization|Model Checking|Java Pathfinder"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64024,"idioma_id":4,"titulo":"Long-chain acyl-CoA synthetase 4 is regulated by phosphorylation","resumen":"Long chain acyl CoA synthetase 4 (Acsl4) is a key enzyme in steroidogenesis. It participates in steroid synthesis through of arachidonic acid release and Steroidogenic Acute Regulatory protein (StAR) induction.Acsl4 prefers arachidonic acid as substrate and acts probably as a homodimer. In steroidogenic cells, it has been demonstrated that Acsl4 is a high turnover protein located mainly in mitochondrial-associated membrane fraction (MAM) bound to other proteins and that it is newly synthesized by hormone stimulation. The synthesis of Acsl4 constitutes an early step in steroidogenesis.In the steroid synthesis process, activation of kinases plays a very important role. For this reason, the aim of this work was to study Acsl4 as a possible phosphoprotein and try to elucidate the role of its phosphorylation.We have determined for the first time that Acsl4 is a phosphoprotein whose phosphorylation is hormone-dependent. We also demonstrated that Acsl4 acts effectively as a dimer and that phosphorylation occurs after dimer formation.Studies in vitro demonstrated that Acsl4 is a substrate of both PKA and PKC and its phosphorylation by these kinases regulates its activity.","palabras_clave":"ACSL4|PHOSPHORYLATION|STEROIDOGENESIS|ACYL-COA SYNTHETASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64025,"idioma_id":4,"titulo":"Selective charge transfer in donor\/acceptor systems bridged by Tröger base derivatives","resumen":"Recently, unusual charge transfer properties of two triarylamines bridged through an aliphatic bridge (Tröger base) have been reported. The donor\/acceptor couple was shown to have an important electronic coupling between its centers for the hole transfer, although in the absence of a pi linker. In contrast, we here show, by combining first principles calculations with experimental studies, that the bridge has a practically null role in coupling the electron transfer. This sharp contrast might present an interesting potential in the design of molecular devices.","palabras_clave":"donor\/acceptor systems"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64026,"idioma_id":4,"titulo":"Old Players with a Newly Defined Function: Fra-1 and c-Fos Support Growth of Human Malignant Breast Tumors by Activating Membrane Biogenesis at the Cytoplasm","resumen":"    A shared characteristic of tumor cells is their exacerbated growth. Consequently, tumor cells demand high rates of    phospholipid synthesis required for membrane biogenesis to support their growth. c-Fos, in addition to its AP-1    transcription factor activity, is the only protein known up to date that is capable of activating lipid synthesis in normal and    brain tumor tissue. For this latter activity, c-Fos associates to the endoplasmic reticulum (ER) through its N-terminal domain    and activates phospholipid synthesis, an event that requires it Basic Domain (BD) (aa 139?159). Fra-1, another member of    the FOS family of proteins, is over-expressed in human breast cancer cells and its BD is highly homologous to that of c-Fos    with two conservative substitutions in its basic amino acids. Consequently, herein we examined if Fra-1 and\/or c-Fos    participate in growth of breast cancer cells by activating phospholipid synthesis as found previously for c-Fos in brain    tumors. We found both Fra-1 and c-Fos over-expressed in .95% of human ductal breast carcinoma biopsies examined    contrasting with the very low or undetectable levels in normal tissue. Furthermore, both proteins associate to the ER and    activate phospholipid synthesis in cultured MCF7 and MDA-MB231 breast cancer cells and in human breast cancer samples.    Stripping tumor membranes of Fra-1 and c-Fos prior to assaying their lipid synthesis capacity in vitro results in nonactivated    lipid synthesis levels that are restored to their initial activated state by addition of Fra-1 and\/or c-Fos to the assays.    In MDA-MB231 cells primed to proliferate, blocking Fra-1 and c-Fos with neutralizing antibodies blocks lipid-synthesis    activation and cells do not proliferate. Taken together, these results disclose the cytoplasmic activity of Fra-1 and c-Fos as    potential targets for controlling growth of breast carcinomas by decreasing the rate of membrane biogenesis required for    growth.","palabras_clave":"BREAST TUMORS|c-Fos|Fra-1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64027,"idioma_id":4,"titulo":"Seasonal variation in feeding habits and diet selection by wild boars in a semi-arid environment of Argentina","resumen":"The wild boar, Sus scrofa, was first introduced for  hunting purposes in Argentina in 1906 and presently occupies a  wide range of habitats. Understanding the food habits of invasive  species is important for predicting the effects of animal  food consumption on the environment and on human activities,  such as farming. The wild boar is an omnivorous, opportunistic  species whose diet is determined by the relative abundance of  different types of foods. In general, the wild boar?s diet has  been widely studied in the world, both as a native and invasive  species, but little is known regarding food resource selection in  the Monte Desert biome. Our study assessed the seasonal  variation in the diet of wild boars, as well as the nutritional  quality of consumed items. Further, we determined the diet  selection of this species. Diet analyses were based on faecal  samples collected over two seasons (wet and dry) in 1 year.  Herbs were the most frequently consumed food item, with wild  boars showing a selection for them in both seasons. The wild  boar uses food resources according to seasonal availability  (larger trophic niche breadth under higher plant diversity, as  in the wet season). In turn, within each season, it selects items  of high forage quality and high carbohydrate contents. In  conclusion, this foraging strategy enables wild boar to maximize  energy budget through food selection in order to survive  in a semi-arid environment such as the Monte Desert.","palabras_clave":"Sus scrofa |Invasive species |Diet selection| Arid lands"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64028,"idioma_id":4,"titulo":"Multi-scale patterns of habitat use by wild boar in the Monte Desert of Argentina","resumen":"A large number of protected areas worldwide have been impacted by biological invasions, threatening the biodiversity they  aim to protect. The wild boar (Sus scrofa) is one of the most threatening invasive species in Argentina, already occupying  many ecoregions, including the central Monte Desert. However, there are no studies regarding the use that wild boars make of  this invaded biome and what factors (climate or landscape) determine or contribute to the establishment of this species. The  objectives of this study were to assess habitat use of the wild boar at spatial and temporal scales in the central Monte Desert, and  to assess if climatic factors influence its abundance. Our results show that, at habitat-level the wild boar exhibited preferences  for a particular habitat (Larrea shrubland) for feeding. At microhabitat-level, we found a positive association between herb  cover and wild boar presence. In addition, we found a strong and positive association between the number of days with low  temperatures and the number of wild boar signs registered. Therefore, we consider that in the central Monte Desert, habitat  selection by wild boars is most likely determined by a maximization of food intake and a minimization of exposure to high  temperature.","palabras_clave":"Sus scrofa|Arid lands|Ecology|Spatial use"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64029,"idioma_id":4,"titulo":"Outbreak of a Cluster with Epidemic Behavior due to Serratia marcescens after colistin administration in a hospital setting","resumen":"Serratia marcescens causes health care-associated infections with important morbidity and mortality. Particularly, outbreaks produced by multidrug-resistant isolates of this species, which is already naturally resistant to several antibiotics, including colistin, are usually described with high rates of fatal outcomes throughout the world. Thus, it is important to survey factors associated with increasing frequency and\/or emergence of multidrug-resistant S. marcescens nosocomial infections. We report the investigation and control of an outbreak with 40% mortality due to multidrug-resistant S. marcescens infections that happened from November 2007 to April 2008 after treatment with colistin for Acinetobacter baumannii meningitis was started at hospital H1 in 2005. Since that year, the epidemiological pattern of frequently recovered species has changed, with an increase of S. marcescens and Proteus mirabilis infections in 2006 in concordance with a significant decrease of the numbers of P. aeruginosa and A. baumannii isolates. A single pulsed-field gel electrophoresis (PFGE) cluster of S. marcescens isolates was identified during the outbreak. When this cluster was compared with S. marcescens strains (n = 21) from 10 other hospitals (1997 to 2010), it was also identified in both sporadic and outbreak isolates circulating in 4 hospitals in Argentina. In132::ISCR1::blaCTX-M-2 was associated with the multidrug-resistant cluster with epidemic behavior when isolated from outbreaks. Standard infection control interventions interrupted transmission of this cluster even when treatment with colistin continued in several wards of hospital H1 until now. Optimizing use of colistin should be achieved simultaneously with improved infection control to prevent the emergence of species naturally resistant to colistin, such as S. marcescens and P. mirabilis.","palabras_clave":"Serratia marcescens|outbreak|epidemic cluster|blaCTX-M2 gene"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64030,"idioma_id":4,"titulo":"Identification and quantification of the interaction mechanisms  between the cationic surfactant HDTMA-Br and montmorillonite","resumen":"Abstract?Several detailed studies have been done on the characterization of organoclays and the type of structures developed when they interact with alkylammonium molecules. Few published contributions exist, however, on the distribution of surfactant within the organoclays and the mechanism by which they are intercalated. Also, although X-ray photoelectron spectroscopy (XPS) is a suitable technique for the study of the surface characteristics of organoclays, very few such XPS studies have been carried out. With the aim of contributing to a better understanding of the intercalation process, a series of organoclays was synthesized using a montmorillonite and the cationic surfactant hexadecyltrimethylammonium bromide (HDTMABr), with an increasing surfactant load of between 0.2 and 4.0 times the cation exchange capacity of the starting clay. By means of XPS, zeta potential, and thermal analysis techniques, distinguishing the strongly interacting fraction from the weakly interaction fraction of the adsorbed surfactant molecules waspossible. Adsorption isotherms of each of these processes were constructed and then adjusted using the Langmuir and Dubinin-Radusquevich adsorption models. Three types of interaction between the surfactant and the clay were identified and described qualitatively and quantitatively. Two of these interactions, strong and weak, involved the hexadecyltrimethylammonium cation (HDTMA+). The third was a weak interaction involving the ion pair HDTMA+Br. The results of this study may be useful for the comprehensive design of organoclays with specific physicochemical properties according to the application for which they are destined.","palabras_clave":"surfactant HDTMA-Br. | Montmorillonite|mechanism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64031,"idioma_id":5,"titulo":"Desarrollo de un vehículo experimental con sistema híbrido de energía","resumen":"La primera competencia latinoamericana de vehículos solares, en la categoría la Ruta Solar, a través deldesierto de Atacama, Chile (2011) dio origen a un proyecto interdisciplinario para fomentar el uso deenergías sustentables aplicadas al transporte urbano, sin empleo de combustibles fósiles nocivos almedio ambiente. Se apuntó al desarrollo de un vehículo con mínimo consumo de energía para su transporte,liviano, estable, de bajo costo y emisión cero basado en la combinación de energía solar fotovoltaicay energía eléctrica obtenida mediante un generador accionado por tracción humana, ambas almacenadasen una batería. En este trabajo se presentan los aspectos inherentes a las etapas del proyecto yejecución de un vehículo híbrido experimental, denominado Pampa Solar. Ello comprende la concepcióny dimensionado de la estructura resistente, adopción de la configuración de celdas solares, dimensionadoy selección de la batería, generador trifásico y desarrollo de la instrumentación electrónica; respetandolas bases de la competencia y las cargas pertinentes. El análisis de los resultados obtenidos de lossistemas eléctrico, electrónico, mecánico y energético del vehículo, durante la competencia, demostraronun desempeño confiable, obteniendo el premio al uso más eficiente de la energía solar.Palabras claves: energías sustentables, vehículo experimental, energía solar fotovoltaica, celdas solares,competencia La ruta Solar.","palabras_clave":"Vehículo híbrido experimental|energía solar"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64032,"idioma_id":5,"titulo":"Los académicos argentinos y sus perspectivas sobre la autonomía y el co-gobierno universitario","resumen":"RESUMEN La tradición universitaria argentina a partir de la Reforma de 1918, se construyó sobre los pilares de la autonomía y del cogobierno universitario; es decir a salvo de los vaivenes del poder gubernamental de turno y de cualquier otro factor de poder socioeconómico, garantizando mecanismos democráticos para la toma de decisiones. Al igual que el resto de sus homólogas de la región, las universidades argentinas estuvieron sometidas a lo largo del siglo XX, a cambios en las condiciones políticas, sociales y culturales y como consecuencia de ello, los conceptos de cogobierno y autonomía  fueron modificándose, asumiendo diferentes expresiones y forjando experiencias tan variadas como complejas de comprender. En el caso argentino los debates más recientes sobre las restricciones a la autonomía universitaria y a la libertad académica, se inician con las políticas universitarias de los ?90 que legitiman los procesos de evaluación y acreditación universitaria con la creación de la CONEAU y la sanción de la Ley de Educación Superior (LES). Así la confianza pública en las instituciones universitarias será reemplazada por nuevas formas de rendición de cuenta con el consecuente impacto en el principio autonómico. En esta nueva visión, la evaluación vendría a ser un modo de restablecer la confianza en la universidad, sin              que esto signifique aumentar el grado de libertad de las instituciones.Evidencias de ello es el marcado intervencionismo gubernamental de los últimos veinte años en la producción e implementación de las políticas para el sector,  que muestran el abandono por parte del gobierno nacional  de  un rol pasivo y focalizado más bien en lo administrativo y la adopción de un perfil de fuerte liderazgo que condujo a la progresiva devaluación de los órganos de gobierno de las universidades (Consejos Superiores y CIN). Aún cuando el quiebre del año 2001 haya significado el abandono del paradigma neoliberal por parte del gobierno - que para las UUNN implico recomposición salarial y mayor presupuesto- la activa presencia de la Secretaria de Políticas Universitarias se evidencia en la importancia de los recursos que maneja y la  generación  permanente de  programas y proyectos.  Las restricciones presupuestarias y la necesidad de adecuarse a las tendencias reformistas globales, fueron volviendo a las instituciones cada vez más dependientes del poder central limitándose su autonomía a la administración de los escasos recursos, perdiendo estas la iniciativa en la definición de políticas claves de la vida universitaria: políticas de acceso y retención, desarrollo de áreas de investigación, fortalecimiento de la docencia, líneas prioritarias de extensión universitaria, etc.. Esta situación se ve reforzada con la vigencia dentro del gobierno del sistema de educación superior de una multiplicidad de órganos de gobierno (CPRES, Consejo de Universidades, CONEAU, CIN, CRUP) que fueron fragmentando cada vez más los espacios decisionales a favor de la tecno-burocracia del poder central (S.P.U.). Frente a este escenario, nos interrogamos acerca del perfil que adopta el sistema de cogobierno como principio fundante de la democracia universitaria. Algunas evidencias muestran que la tendencia fragmentaria macro se reproduce hacia el interior de la universidad, desplazándose la toma de decisiones colegiadas hacia figuras  ejecutivas tales como Rectores y Decanos. También parece haber perdido substancia la representatividad, funciones y atribuciones de los ?representantes?, cuya actividad se reduce  más a la resolución de temas académico-administrativos puntuales, a tejer alianza y retribuir favores y lealtades, alejado de los espacios en donde se define la agenda y se toman decisiones de política universitaria.De cara a esta situación y pensando en la construcción de proyectos universitarios alternativos se torna imprescindible la producción de conocimiento sobre los principios de autonomía y cogobierno. Con este propósito este trabajo realizará un aporte de las condiciones actuales que presentan el ejercicio de la autonomía y el cogobierno universitario en la argentina. A tal fin la ponencia se estructura en dos partes mostrando, por un lado, las estrategias gubernamentales de reforma de los últimos veinte años y las tendencias que actúan sobre la universidad, forzando cambios y alterando sus condiciones generales de funcionamiento. Por otro, la percepción de académicos argentinos sobre los principios de autonomía y cogobierno, producto de un estudio cualitativo a escala nacional que involucra a 80 profesores de las UUNN. (PICT redes 1890- CAP  The Changing Academic Profession Project?).SUMMARYThe objetive of the article is to analyze the current conditions of university autonomy in Argentina as well as the ways its cogovernment system is carried out. With is purpose, the article will show, on one hand, the governmental strategies of the higher education reform of the last twenty years and the general trends from culture, economy and politics that act over the university, promoting changes and altering its normal functioning. On the other hand, the perception of Argentine academics regarding autonomy and cogoverment, obtained as a result of a national qualitative study involving 80 teachers from National Universities (PICT redes 1890-CAP The Changing Academic Profession Project).","palabras_clave":"UNIVERSIDAD|ACADEMICOS|AUTONOMIA|COGOBIERNO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64033,"idioma_id":4,"titulo":"Influence of hairy root ecotypes on production of tropane alkaloids in Brugmansia candida","resumen":"Hyoscyamine and scopolamine are tropane alkaloids widely applied in medicine. Differences in alkaloids production and growth kinetics were observed for Argentinean and Colombian ecotypes of Brugmansia candida hairy roots. The aim of this work was to analyze the production of key intermediates in both ecotypes in order to determine differences in the biosynthetic pathway of tropane alkaloids. Additionally, rolC gene expression was analyzed to determine its correlation with hairy root growth. The results showed a higher accumulation of polyamines in Colombian hairy roots suggesting that there may be a rate limiting enzyme in the last steps of the hyoscyamine biosynthesis. Additionally, rolC gene expression was correlated with an improvement hairy root growth supporting the involvement of rol genes as growth modulators and suggesting that metabolic engineering approaches by rolC manipulation may be useful for the development of more efficient B. candida hairy root cultures for biotechnological applications.","palabras_clave":"HYOSCYAMINE|SCOPOLAMINE|6B-HYDROXYHYOSCYAMINE|HYOSCYAMINE 6B-HYDROXYLASE|POLYAMINES|ROLC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64034,"idioma_id":4,"titulo":"Simultaneous correlation of Complementary Set of Sequences using a transpose  generation approach","resumen":"Complementary sets of sequences are currently being applied to signal coding, radar, and multi-user systems, among others. Their particular  mathematical properties make them adequate for multi-emission and noisy environments. Nowadays sustained efforts are being devoted to reduce the  calculations involved in the generation and\/or correlation of these signals by means of recursive algorithms. Some authors have proposed efficient algorithms that are based on modular architectures made up of adders, multipliers and delays. This work introduces a new approach to correlation algorithms of complementary sets of sequences, which is based on a transposition of the generation process. This approach allows to notoriously reduce calculations, and enables the simultaneous correlation of $M$ sequences, without adopting time multiplexing schemes or complex parallel implementations. The correlation algorithm is theoretically demonstrated and its calculation performance is evaluated in a hardware reconfigurable platform. A comparison with other algorithms is included, considering the amount of calculations as a function of the length of the sequences.","palabras_clave":"Complementary set of sequences|Correlation|Efficient architectures"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64035,"idioma_id":4,"titulo":"Sorption characteristics of rosehip, apple and tomato pulp formulations as determined by gravimetric and hygrometric methods","resumen":"The sorption characteristics of three formulations based on rosehip, apple and tomato pulp, added with saccharides, and aimed at preparing fruit leathers, were studied. Desorption isotherms were determined at 20 and 40 °C, both by the static gravimetric and the hygrometric method. Experimental isotherms were all J-shaped, as expected for rich-sugar matrices. A model previously developed for apple leather isotherms, the GAB equation and the Halsey model were fitted to the gravimetric data. Hygrometric isotherms exhibited a typical behavior at high moisture contents although, at lower values, the water activity readings were consistently higher than in the gravimetric method, and were never below 0.363. Apparently, the low-moisture, high-sugar samples behave as non-hygroscopic materials in the short times allowed by the hygrometric measurement, possibly due to the presence of crystallized sugars. On these grounds, the fast hygrometric method appears to be unsuitable to measure the water activity of such samples.","palabras_clave":"High-sugar foods| Fruits|Isotherm| Water activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64036,"idioma_id":4,"titulo":"In vitro antistaphyloccocal effects of a novel 45S5 bioglass\/agar-gelatin biocomposite films","resumen":"To assess the antibacterial efficacy of new composite materials developed from microparticles of 45S5 bioactive glass (BG) and agar-gelatin films. METHODS AND RESULTS: In vitro antibacterial activity was evaluated against Staphylococcus spp. because of the importance of this pathogen in damaged tissues and in failures associated with biomaterial implants. To our knowledge, this is the first paper reporting on the suitable combination of BG and agar-gelatin for bioactive and antibacterial films. Bacterial suspensions up or below 10 reflecting situations of wound infection and of non-infection respectively were prepared and then put in contact with the biomaterials at 37°C. After 24 and 48 h of incubation, the pH value was measured and the staphylococci strains viability was determined by counting in Mueller-Hinton agar plates. Moreover, the biomaterials were prepared for observation under scanning electron microscopy (SEM). Biocomposites (BCs) showed a strong antibacterial effect against all staphylococci strains tested. Some differences were found depending on the strain, the inoculum size and the contact time. This effect was correlated with an alkalinization of the media. By SEM analyses, no bacterial presence was observed on the surface of BCs in any of the cell concentrations tested at any time.CONCLUSIONS: Overall, the coating of 45S5 BG on agar-gelatin films promoted BCs with strong antistaphylococcal activity. The effect was efficient under bacterial concentration up or below 10. Additionally, none of the strains were found on BCs surfaces. SIGNIFICANCE AND IMPACT OF STUDY: 45S5 bioglass\/agar-gelatin biocomposite films are reported for the first time. The results suggest a potential application as wound dressing.","palabras_clave":"ANTIBACTERIAL ACTIVITY|BIOACTIVE GLASS|BIOPOLYMERS|BIOCOMPOSITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64037,"idioma_id":4,"titulo":"'Stability and Relaxation Mechanisms of Citric Acid Coated Magnetite Nanoparticles for Magnetic Hyperthermia","resumen":"Magnetite (Fe3O4) nanoparticles are proper materials for Magnetic Fluid   Hyperthermia applications whenever these conjugate stability at   physiological (neutral pH) medium and high specific dissipation power.   Here, magnetite nanoparticles 9 - 12 nm size, electrostatically   stabilized by citric acid coating, with hydrodynamic sizes in the range   17 - 30 nm and well dispersed in aqueous solution were prepared using a   chemical route. The influence of media acidity during the adsorption of   citric acid (CA) on the suspensions long term stability was   systematically investigated. The highest content of nanoparticles in a   stable suspension at neutral pH is obtained for coating performed at pH =   4.58 corresponding to the larger amount of CA molecules adsorbed by one   carboxylate link. Specific absorption rates (SAR) of various magnetite   colloids, determined calorimetrically at a radio frequency field of 265   kHz and field amplitude of 40.1 kA\/m are analyzed in terms of structural   and magnetic colloid properties. Larger dipolar interactions, lead to   larger Néel relaxation times, in some cases larger than Brown relaxation   times, which in the present case enhanced magnetic radio frequency   heating. The improvement of suspension stability results in a decrease   of SAR values, and this decrease is even large in comparison with   uncoated magnetite nanoparticles. This fact is related to interactions   between particles.","palabras_clave":"Nanoparticle|Ferrofluid|Magnetic Hyperthermia|Magnetite|Citric acid coating"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64038,"idioma_id":5,"titulo":"A Robust Model Predictive Control For Grid- Connected Voltage-Source Inverters","resumen":"Model predictive control is a new alternative in the control of power converters. The practical application of this controller demands a correct compensation of the computation delay. This compensation requires an estimation of the load current, usually attained in an open-loop manner, i. e. without error feedback of the model parameters. However, this strategy gives rise to diverse perturbations in the waveform, when there is a certain amount of parameter variation. In this work, the effects of this variation are analyzed and a way to reduce the distortion is proposed, using a Luenberger observer. This new approach is called Robust Model Predictive Control. The proposal is applied to a grid-connected voltage-source inverter. To validate the proposal, simulations are included in the paper","palabras_clave":"Control predictivo|inversor trifásico|marco de referencia estacionario|observadores de estado|variación de parámetros"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64039,"idioma_id":4,"titulo":"Radiation budget changes with dry forest clearing in temperate Argentina","resumen":"Land cover changes may affect climate and the energy balance of the Earth through their influence on the greenhouse gas composition of the atmosphere (biogeochemical effects) but also through shifts in the physical properties of the land surface (biophysical effects). We explored how the radiation budget changes following the replacement of tem-perate dry forests by crops in central semiarid Argentina and quantified the biophysical radiative forcing of this transformation. For this purpose, we computed the albedo and surface temperature for a 7-year period (2003\u20132009) from MODIS imagery at 70 paired sites occupied by native forests and crops and calculated the radiation budget at  the tropopause and surface levels using a columnar radiation model parameterized with satellite data. Mean annual black-sky albedo and diurnal surface temperature were 50% and 2.5°C higher in croplands than in dry forests. These contrasts increased the outgoing shortwave energy flux at the top of the atmosphere in croplands by a quarter (58.4 vs. 45.9 W m2) which, together with a slight increase in the outgoing longwave flux, yielded a net cooling of 14 Wm2. This biophysical cooling effect would be equivalent to a reduction in atmospheric CO2of 22 Mg C  ha1, which involves approximately a quarter to a half of the typical carbon emissions that accompany deforestation in these ecosystems. We showed that the replacement of dry forests by crops in central Argentina has strong biophys-ical effects on the energy budget which could counterbalance the biogeochemical effects of deforestation. Underesti-mating or ignoring these biophysical consequences of land-use changes on climate will certainly curtail the effectiveness of many warming mitigation actions, particularly in semiarid regions where high radiation load and smaller active carbon pools would increase the relative importance of biophysical forcing.","palabras_clave":"albedo change|deforestation|energy budget|NDVI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64040,"idioma_id":4,"titulo":"How many taxa can be recognized within the complex Tillandsia capillaris (Bromeliaceae, Tillandsioideae)? Analysis of the available classifications using a multivariate approach","resumen":"Tillandsia capillarisRuiz & Pav., which belongs to the subgenus Diaphoranthemais distributed in Ec-uador, Peru, Bolivia, northern and central Argentina, and Chile, and includes forms that are difficult   to circumscribe, thus considered to form a complex. The entities of this complex are predominantly small-sized epiphytes, adapted to xeric environments. The most widely used classification defines 5 forms   for this complex based on few morphological reproductive traits: T. capillarisRuiz & Pav. f. capillaris, T. capillarisf. incana(Mez) L.B. Sm., T. capillarisf. cordobensis(Hieron.) L.B. Sm., T. capillarisf. hieronymi (Mez) L.B. Sm. and T. capillarisf. virescens(Ruiz & Pav.) L.B. Sm. In this study, 35 floral and vegetative characters were analyzed with a multivariate approach in order to assess and discuss different proposals for classification of the T. capillariscomplex, which presents morphotypes that co-occur in central and northern Argentina. To accomplish this, data of quantitative and categorical morphological characters of flowers and leaves were collected from herbarium specimens and field collections and were analyzed with statistical multivariate techniques. The results suggest that the last classification for the complex seems more comprehensive and three taxa were delimited: T. capillaris(=T. capillaris f. incana-hieronymi), T. vire-scens s. str.(=T. capillarisf.cordobensis) and T. virescens s. l.(=T. capillaris f. virescens). While T. capillarisand T. virescens s. str.co-occur, T. virescens s. l.is restricted to altitudes above 2000 m in Argentina. Characters previously used for taxa delimitation showed continuous variation and therefore were not useful. New di-agnostic characters are proposed and a key is provided for delimiting these three taxa within the complex.","palabras_clave":"TILLANDSIA CAPILLARIS|BROMELIACEAE|SPECIES COMPLEX|MORPHOMETRY|DISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64041,"idioma_id":4,"titulo":"A Low-Complexity Decision Feedforward Equalizer Architecture for High-Speed Receivers on Highly Dispersive Channels","resumen":"This paper presents an improved decision feedforward equalizer (DFFE) for high speed receivers in the presence of highly dispersive channels. This decision-aided equalizer technique has been recently proposed for multigigabit communication receivers, wherethe use of parallel processing is mandatory. Well-known parallel architectures for the typical decision feedback equalizer (DFE) have a complexity that grows exponentially with the channel memory. Instead, the new DFFE avoids that exponential increase in complexity by using tentative decisions to cancel iteratively the intersymbol interference (ISI). Here, we demostrate that the DFFE not only allows to obtain a similar performance to the typical DFE but it also reduces the compelxity in channels with large memory. Additionally, we propose a theoretical approximation for the error probability in each iteration. In fact, when the number of iteration increases, the error probability in the DFFE tends to approach the DFE. These benefits make the DFFE an excellent choice for the next generation of high-speed receivers.","palabras_clave":"ECUALIZER|LOW COMPLEXITY|PARALLEL|REALIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64042,"idioma_id":4,"titulo":"Efficient Parallel Carrier Recovery for Ultrahigh Speed Coherent QAM Receivers with Application to Optical Channels","resumen":"This work presents a new efficient parallel carrier recovery architecture suitable for ultrahigh speed intradyne coherent optical receivers (e.g., &#8805;100&#8201;Gb\/s) with quadrature amplitude modulation (QAM). The proposed scheme combines a novel low-latency parallel digital phase locked loop (DPLL) with a feedforward carrier phase recovery (CPR) algorithm. The new low-latency parallel DPLL is designed to compensate not only carrier frequency offset but also frequency fluctuations such as those induced by mechanical vibrations or power supply noise. Such carrier frequency fluctuations must be compensated since they lead to higher phase error variance in traditional feedforward CPR techniques, significantly degrading the receiver performance. In order to enable a parallel-processing implementation in multigigabit per second receivers, a new approximation to the DPLL computation is introduced. The proposed technique reduces the latency within the feedback loop of the DPLL introduced by parallel processing, while at the same time it provides a bandwidth and capture range close to those achieved by a serial DPLL. Simulation results demonstrate that the effects caused by frequency deviations can be eliminated with the proposed low latency parallel carrier recovery architecture.","palabras_clave":"Low-latency|Digital|DPLL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64043,"idioma_id":4,"titulo":"Compensation of Laser Frequency Fluctuations and Phase Noise in 16-QAM Coherent Receivers","resumen":"Frequency fluctuations caused by mechanical vibrations, power supply noise, and other mechanisms are detrimental to the phase estimator performance in high speed intradyne coherent optical receivers. In this letter, we propose the use of a low-latency parallel digital phase lock loop in combination with common feed-forward carrier phase recovery algorithms in order to compensate both the phase noise and laser frequency fluctuation effects on 16-quadrature amplitude modulation receivers. Numerical results demonstrate the excellent behavior of the proposed two-stage carrier recovery scheme.","palabras_clave":"Coherent|Frequency|Compensate"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64044,"idioma_id":4,"titulo":"Long-term effects of maternal separation on chronic stress response suppressed by amitriptyline treatment","resumen":"The early-life environment has many long-term effects on mammals. Maternal interaction and early stressful events may affect regulation of the HPA axis during adulthood, leading to differential glucocorticoid secretion in response to stressful situations. These adverse experiences during postnatal development may even sensitize specific neurocircuits to subsequent stressors. Later in life, the overreaction of the HPA axis to stress can constitute a risk factor for metabolic and mental diseases. As tricyclic antidepressants are known to correct glucocorticoid hypersecretion during depression, we treated maternally separated animals with amitriptyline, at a lower dose than habitually used in depression models, to prevent the response to chronic stress during adulthood. Male Wistar rats were separated from the mother for 4.5&#8201;h every day for the first 3 weeks of life. From postnatal day 50, animals were subjected to chronic variable stress during 24&#8201;d (five types of stressors at different times of day). During the stress, protocol rats were orally administered amitriptyline (5&#8201;mg\/kg) daily. We observed that maternal separation caused a reduction in plasma ACTH levels (p&#8201;<&#8201;0.05), but evoked hypersecretion of corticosterone (p&#8201;<&#8201;0.05) when it was combined with stress in adulthood. This rise was completely prevented by antidepressant treatment with amitriptyline.","palabras_clave":"STRESS|AMITRIPTILINE|DEPRESSION|CORTICOSTERONE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64045,"idioma_id":4,"titulo":"Preparation and Characterization of Three Different Derivatized Potato Starches","resumen":"The use of native starch as a thermoplastic polymer is limited by its fragility and high water absorp- tion. Due to the presence of several hydroxyl groups in its structure, water acts as a natural plasticizer of starch, modifying its properties. It is necessary to chemically modify starch molecules by replacing hydroxyl groups with other functional groups to reduce water absorption. Chemical modi&#64257;cation of starch granules also alters its swelling and gelatinization behavior. In this contribution we describe the chemical modi&#64257;cation of starch and its in&#64258;uence on its hydrophilicity and heat resistance. Acetic acid, maleic anhydride and octanoyl chloride were used as derivatizing reagents. The effectiveness of the treatments was evaluated by means of infrared spectroscopy. Different tests were conducted in order to evaluate the in&#64258;uence of the different chemical modi&#64257;cations on starch structure and properties. Results showed that the treatments effectively reduced starch moisture susceptibility, while substantially altering other properties such as amylose content, swelling power, solubility, and heat resistance. Finally, &#64257;lms were prepared from native and derivatized starch and their sur- face polarity was evaluated.","palabras_clave":"BIOPOLYMERS|STARCH|DERIVATIZED STARCH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64046,"idioma_id":4,"titulo":"Lipid storage in High-Altitude Andean Lakes extremophiles and its mobilization under stress conditions in Rhodococcus sp. A5, a UV-resistant actinobacterium","resumen":"The production of triacylglycerols (TAG) or wax esters (WS) seems to be a widespread feature among extremophile bacteria living in high-altitude Andean Lakes (HAAL), Argentina. Twelve out of twenty bacterial strains isolated from HAAL were able to produce TAG or WS (between 2 and 17 % of cellular dry weight) under nitrogen-limiting culture conditions. Among these strains, the extremophile Rhodococcus sp. A5 accumulated significant amounts of TAG during growth on glucose (17 %, CDW) and hexadecane (32 %, CDW) as sole carbon sources. The role of accumulated TAG in the response to carbon starvation, osmotic stress, UV-radiation and desiccation was investigated in Rhodococcus sp. A5 using an inhibitor of TAG degradation. Cells degraded TAG during these stresses in the absence of the inhibitor. The inhibition of TAG mobilization affected cell survival during osmotic stress only during the initial growth stage. Little or no surviving cells were observed after carbon starvation, UV-treatment and desiccation, when TAG mobilization was inhibited. These results suggested that TAG metabolism is relevant for the adaptation and survival of A5 cells under carbon starvation, osmotic stress and UV irradiation, and essential under desiccation conditions, which prevail in HAAL environments.","palabras_clave":"HAAL|EXTREMOPHILES|Triacylglycerol|RHODOCOCCUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64047,"idioma_id":4,"titulo":"Ruthenium polypyridyl phototriggers: from beginnings to perspectives","resumen":"Octahedral Ru(II) polypyridyl complexes constitute a superb platform to devise photoactive triggers capable of delivering entire molecules in a reliable, fast, efficient and clean way. Ruthenium coordination chemistry opens the way to caging a wide range of molecules, such as amino acids, nucleotides, neurotransmitters, fluorescent probes and genetic inducers. Contrary to other phototriggers, these Ru-based caged compounds are active with visible light, and can be photolysed even at 532&#8201;nm (green), enabling the use of simple and inexpensive equipment. These compounds are also active in the two-photon regime, a property that extends their scope to systems where IR light must be used to achieve high precision and penetrability. The state of the art and the future of ruthenium polypyridyl phototriggers are discussed, and several new applications are presented.","palabras_clave":"ruthenium |caged compounds |neurotransmitters |glutamate |gene inducer |lac operon"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64048,"idioma_id":5,"titulo":"Costos Ambientales y Eficiencia Productiva en la Producción Agraria del Partido de Pergamino","resumen":"Mediante un modelo de frontera de producción estocástica se analizó el nivel de eficiencia técnica para la producción de soja, maíz y trigo en empresas agropecuarias del partido de Pergamino, Buenos Aires, Argentina. El costo asociado a tres indicadores ambientales: balances de nutrientes, balance de materia orgánica y erosión hídrica, se consideró como parte de los insumos de producción. En promedio, la soja 1ra es el cultivo con costo ambiental más elevado, con un costo ambiental 53u$s\/ha mayor que en la secuencia trigo\/soja y 76 u$s\/ha mayor que en maíz. Se estimó un nivel de eficiencia promedio del 85%. Si bien este valor implica la posibilidad de aumentar un 15% la producción para un mismo nivel de insumos, es un valor elevado si se lo compara con mediciones realizadas en otros sistemas agrícolas extensivos. Aunque un alto nivel de eficiencia es consistente con un buen uso de los recursos, los resultados sugieren que es posible reducir los costos ambientales de los sistemas de producción mediante cambios en la combinación de cultivos. En la actualidad existe una fuerte predominancia del cultivo de soja en toda la región agrícola de Argentina. Una mayor superficie de cultivos con menores costos ambientales podría conseguirse mediante la implementación de políticas que fomenten estas producciones.","palabras_clave":"economia|produccion|fronteras estocasticas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64049,"idioma_id":4,"titulo":"Calbindin D28k and Calretinin in chicken inner retina during postnatal development and neuroplasticity by dim red light.","resumen":"Members of the family of calciumbinding proteins (CBPs) are involved in the buffering ofcalcium (Ca21) by regulating how Ca21 can operatewithin synapses or more globally in the entire cytoplasmand they are present in a particular arrangement in alltypes of retinal neurons. Calbindin D28k and calretininbelong to the family of CBPs and they are mainly coexpressedwith other CBPs. Calbindin D28k is expressedin doubles cones, bipolar cells and in a subpopulation ofamacrine and ganglion neurons. Calretinin is present inhorizontal cells as well as in a subpopulation of amacrineand ganglion neurons. Both proteins fill the soma at theinner nuclear layer and the neuronal projections at theinner plexiform layer.Moreover, calbindin D28k and calretininhave been associated with neuronal plasticity inthe central nervous system. During pre and early postnatalvisual development, the visual system shows highresponsiveness to environmental influences. In this workwe observed modifications in the pattern of stratificationof calbindin immunoreactive neurons, as well as in thetotal amount of calbindin through the early postnatal development.In order to test whether or not calbindin isinvolved in retinal plasticity we analyzed phosphorylatedp38 MAPK expression, which showed a decrease in pp38MAPK, concomitant to the observed decrease of calbindinD28k. Results showed in this study suggest thatcalbindin is a molecule related with neuroplasticity, andwe suggest that calbindin D28k has significant roles inneuroplastic changes in the retina, when retinas arestimulated with different light conditions.","palabras_clave":"Chick|Retina|Neuroplasticity|Calcium binding proteins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64050,"idioma_id":4,"titulo":"Developmental endpoints of chronic exposure to suspected endocrine-disrupting chemicals on benthic and hyporheic freshwater copepods","resumen":"The aims of this study were: (i) to assess if carbamate pesticides and ammonium, widely detected in European freshwater bodies, can be considered ecologically relevant endocrine-disrupting chemicals (EDCs) for benthic and interstitial freshwater copepods; and (ii) to evaluate the potential of copepods as sentinels for monitoring ecosystem health. In order to achieve these objectives, four species belonging to the harpacticoid copepod genus Bryocamptus, namely B. (E.) echinatus, B. (R.) zschokkei, B. (R.) pygmaeus and B. (B.) minutus, were subjected to chronic exposures to Aldicarb and ammonium. A significantdeviation from the developmental time of unexposed control cultures was observed for all the species in test cultures. Aldicarb caused an increase in generation time over 80% in both B. minutus and B. zschokkei, but less than 35% in B. pygmaeus and B. echinatus. Ammonium increased generation time over 33% in B. minutus, and 14, 12 and 3.5% for B. pygmaeus, B. zschokkei and B. echinatus, respectively. On the basis of these results it can be concluded that chronic exposure to carbamate pesticides and ammonium alters the post-naupliar development of the test-species and propose their potential role as EDCs, leaving open the basis to search what are the mechanism underlying. A prolonged developmental time would probably produce a detrimental effect on population attributes, such as age structure and population size. These deviations from a pristine population condition may be considered suitable biological indicators of ecosystem stress, particularly useful to compare polluted to unpolluted reference sites. Due to their dominance in both benthic and interstitial habitats, and their sensitivity as test organisms, freshwater benthic and hyporheic copepods can fully be used as sentinel species for assessing health condition of aquatic ecosystems as required by world-wide water legislation ","palabras_clave":"Copepod|Endocrine disruptor|Aldicarb|developmental time"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64051,"idioma_id":5,"titulo":"Design and Implementation of a Three-Phase DC-AC Converter for Microgrids Based on Renewable Energy Sources","resumen":"This paper proposes a three-phase DC-AC converter for autonomous microgrids based onrenewable energy sources. The microgrid topology offer simple control characteristics, where the AC link does not need a complex voltage controller strategy. The DC-AC converter (three-phase inverter) generates output voltages with very low THD for any type of load. To obtain the before mentioned characteristics for the inverter, this work propose an internal model principle based controller structure in synchronous dqframe, with a reduced sampling and number of poles. With this structure, is possible to considerably improve the stability and dynamic system response, and mainly, the saturation of the low frequency insulating transformer is avoided. Also in this paper, is proposed a straightforward design methodology for the discrete internal model based controller. Inorder to validate the proposed digital controller structure, the design methodology and to demonstrate the steady state and transient performance, experimental results are presented from a 15kVA SVPWM modulated three-phase inverter, fully controlled by a DSC dsPIC30F4011.","palabras_clave":"DC-AC Converter|down-sampled digital  controller|internal model principle|microgrids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64052,"idioma_id":4,"titulo":"Evaluation of rhizobacterial isolates from Argentina, Uruguay and Chile for plant growth promoting characteristics and antagonistic activity towards Rhizoctonia sp. and Macrophomina sp. in vitro","resumen":"This study was conducted over 3 years in soils of Uruguay and Chile and two years in soils of Argentina. 686 bacterial isolates were phenotypically characterized by testing in relation to the presence of plant growth promoting properties: phosphate solubilization, production of siderophores, starch hydrolysis, production of exopolysaccharides and biological control of Macrophomina phaseolina and Rhizoctonia spp. In all samples analyzed, the number of Gram-positive bacteria exceeded that of Gram negative. Ten bacterial isolates were selected for their plant growth promoting properties and API Test and 16S rRNA gene (rDNA). Six of these isolates belong to the genus Pseudomonas, three to the genus Bacillus and one to Janibacter. This is the first report of a strain from the genus Janibacter with promising plant growthpromoting ttributes. The results obtained allow us to improve the microbial germplasm of plant growth promoting bacteria from soils of Chile, Argentina and Uruguay with a view to their potential use in the formulation of mixed inoculants that promote the growth of alfalfa.","palabras_clave":"ALFALFA|PGPR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64053,"idioma_id":4,"titulo":"Antifungal  activity  in  vitro  and  in  vivo  of  extracts  and  lignans  isolated  from  Larrea divaricata  Cav.  against  phytopathogenic  fungus","resumen":"The aim of this study was to evaluate the effectiveness of organic extracts and isolated compounds of the medicinal plant Larrea divaricata Cav. against phytopathogenic fungi. Chloroformic extract showed the  highest antifungal activity and three compounds were identified in this extract: Apigenine-7-methylether (1), nordihydroguaiaretic acid (2) and 3,4-dihydroxy-3,4-dimethoxy-6,7-cyclolignan (3). Compound 3  is described for the first time in the species and exhibited strong antifungal activity in vitro (Fusarium graminearum MIC = 15.6 g\/mL) and in vivo producing decreasing disease on glasshouse-grown wheat  plants reducing damping-off (14% in pre emergency) and Severity Index from 2.6 upto 1.1. In this study it is clear that the application of botanical products from L. divaricata can be effective in the management  of seedling blight and crown rot of wheat.","palabras_clave":"Antifungal activity|Chemical constituents|Larrea divaricata|Organic extracts|Phytopathogenic fungi"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64054,"idioma_id":4,"titulo":"Accelerating embedded image processing for real time: a case study","resumen":"Many image processing applications need real-time performance, while   having restrictions of size, weight and power consumption. Common   solutions, including hardware\/software co-designs, are based on Field   Programmable Gate Arrays (FPGAs). Their main drawback is long   development time. In this work, a co-design methodology for   processor-centric embedded systems with hardware acceleration using   FPGAs is proposed. The goal of this methodology is to achieve real-time   embedded solutions, using hardware acceleration, but achieving   development time similar to that of software projects. Well established   methodologies, techniques and languages from the software domain\u2014such as   Object-Oriented Paradigm design, Unified Modelling Language, and   multithreading programming\u2014are applied; and semiautomatic C-to-HDL   translation tools and methods are used and compared. The methodology is   applied to achieve an embedded implementation of a global vision   algorithm for the localization of multiple robots in an e-learning   robotic laboratory. The algorithm is specifically developed to work   reliably 24\/7 and to detect the robot\u2019s positions and headings even in   the presence of partial occlusions and varying lighting conditions   expectable in a normal classroom. The co-designed implementation of this   algorithm processes 1,600&nbsp;×&nbsp;1,200 pixel images at a rate of 32 fps with   an estimated energy consumption of 17&nbsp;mJ per frame. It achieves a   16×&nbsp;acceleration and 92&nbsp;% energy saving, which compares favorably with   the most optimized embedded software solutions. This case study shows   the usefulness of the proposed methodology for embedded real-time image   processing applications.","palabras_clave":"Real-time image processing|Methodology for hardware\/software co-design in FPGA|High level synthesis|High level modeling|Multiple robot localization|Multithreaded programming|Hardware acceleration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64055,"idioma_id":4,"titulo":"Improving the glycosyltransferase activity of A grobacterium tumefaciens glycogen synthase by fusion of N-terminal starch binding domains (SBDs)","resumen":"Glycogen and starch, the major storage carbohydrate in most living organisms, result mainly from the action of starch or glycogen synthases (SS or GS, respectively, EC 2.4.1.21).SSIII from Arabidopsis thaliana is an SS isoform with a particular modular organization: the C-terminal highly conserved glycosyltransferase domain is preceded by a unique specific region (SSIII-SD) which contains three in tandem starch binding domains (SBDs, named D1, D2 and D3) characteristic of polysaccharide degrading enzymes. N-terminal SBDs have a probed regulatory role in SSIII activity, showing starch binding ability and modulating the catalytic properties of the enzyme. On the other hand, GS from Agrobacterium tumefaciens has a simple primary structure organization, characterized only by the highly conserved glycosyltransferase domain and lacking SBDs.To further investigate the functional role of A. thaliana SSIII-SD, three chimeric proteins were constructed combining the SBDs from A. thaliana with the GS from A. tumefaciens. Recombinant proteins were expressed in and purified to homogeneity from Escherichia coli cells in order to be kinetically characterized. Furthermore, we tested the ability to restore in vivo glycogen biosynthesis in transformed E. coli glgA&#8722; cells, deficient in GS. Results show that the D3-GS chimeric enzyme showed increased capacity of glycogen synthesis in vivo with minor changes in its kinetics parameters compared to GS.","palabras_clave":"GLYCOSYLTRANSFERASE|STARCH BINDING DOMAINS|AGROBACTERIUM|CBM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64056,"idioma_id":4,"titulo":"The use of alternative polyadenylation sites renders &#946;1- integrin mRNA isoforms with differential stability during mammary gland development.","resumen":"Abstract  Integrins are heterodimeric cell surface adhesion receptors that play a critical role in tissue development. Characterization of the full length mRNA encoding the &#946;1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform, 578bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is privileged during lactation, while the shorter is induced after weaning. In 3D cultures, where expression of Itgb1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species that allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, our data identify a new regulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function.","palabras_clave":"HUR|ITGB1|ALTERNATIVE POLYADENYLATION|GENE EXPRESSION REGULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64057,"idioma_id":4,"titulo":"Circular Dichroism and Electron Microscopy Studies In Vitro of 33-mer Gliadin Peptide Revealed Secondary Structure Transition and Supramolecular Organization","resumen":"Gliadin, a protein present in wheat, rye, and barley,  undergoes incomplete enzymatic degradation during  digestion, producing an immunogenic 33-mer peptide,  LQLQPF(PQPQLPY)3  PQPQPF. The special features of  33-mer that provoke a break in its tolerance leading to  gliadin sensitivity and celiac disease remains elusive.  Herein, it is reported that 33-mer gliadin peptide was not  only able to fold into polyproline II secondary structure  but also depending on concentration resulted in conformational transition and self-assembly under aqueous  condition, pH 7.0. A 33-mer dimer is presented as one  initial possible step in the self-assembling process  obtained by partial electrostatics charge distribution calculation and molecular dynamics. In addition, electron  microscopy experiments revealed supramolecular organization of 33-mer into colloidal nanospheres. In the presence of 1 mMsodium citrate, 1 mMsodium borate, 1  mMsodium phosphate buffer, 15 mMNaCl, the nanospheres were stabilized, whereas in water, a linear organization and formation of fibrils were observed. It is  hypothesized that the self-assembling process could be the  result of the combination of hydrophobic effect, intramolecular hydrogen bonding, and electrostatic complementarity due to 33-mer\u2019s high content of proline and  glutamine amino acids and its calculated nonionic  amphiphilic character. Although, performed in vitro,  these experiments have revealed new features of the  33-mer gliadin peptide that could represent an  important and unprecedented event in the early stage of  33-mer interaction with the gut mucosa prior to onset of  inflammation. Moreover, these findings may open new  perspectives for the understanding and treatment of  gliadin intolerance disorders.","palabras_clave":"33-MER|GLIADIN|PEPTIDE|CIRCULAR-DICHROISM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64058,"idioma_id":4,"titulo":"Critical behavior of self- assembled rigid rods on two- dimensional lattices: Bethe- Peierls approximation and Monte Carlo simulations","resumen":"      The critical behavior of adsorbed monomers that  reversibly polymerize into linear chains with restricted orientations relative  to the substrate has been studied. In the model considered here, which is known  as self-assembled rigid rods (SARRs) model, the surface is represented by a  two-dimensional lattice and a continuous orientational transition occurs as a  function of temperature and coverage. The phase diagrams were obtained for the  square, triangular, and honeycomb lattices by means of Monte Carlo simulations  and finite-size scaling analysis. The numerical results were compared with Bethe-Peierls  analytical predictions about the orientational transition for the square and  triangular lattices. The analysis of the phase diagrams, along  with the behavior of the critical average rod lengths, showed that the critical  properties of the model do not depend on the structure of the lattice at low  temperatures (coverage), revealing a quasi-one-dimensional behavior in this  regime. Finally, the universality class of the SARRs model, which has been  subject of controversy, has been revisited.    ","palabras_clave":"Adsortion|Rigid rods|SARRs model"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64060,"idioma_id":5,"titulo":"La apicultura como estrategia de gestión ambiental en la recuperación de la Cuenca del Embalse Guaremal, municipio Peña, estado Yaracuy, Venezuela","resumen":"El objetivo del presente estudio fue proponer estrategias de gestión ambiental para la recuperación de la cuenca del embalse Guaremal mediante el desarrollo de sistemas apícolas. Se realizó un relevamiento florístico alrededor de la cuenca tomando en consideración las especies visitadas por las abejas. Se determinaron las especies melíferas más abundantes de la zona en un área mínima de 0,10 ha definida en 7 transectos de 4m de ancho x 50m de largo, distribuidos a lo largo de toda la cuenca. La superficie fue dividida en tres zonas: baja (transectos 1 y 2), media (transectos 3, 4 y 5) y alta (transectos 6 y 7). Se estableció la densidad absoluta y relativa de cada especie vegetal dentro de cada transecto y entre todos los transectos. Se determinó la composición de la flora en la cuenca del embalse Guaremal, constituida por 77 especies de interés apícola, representadas en 40 familias, siendo las más predominantes: Compositae, Fabaceae, Mimosaceae, Euphorbiaceae y Urticaceae. La aparición de una especie apibotánica colonizadora, Wedelia caracasana, reveló un fuerte impacto antrópico negativo. Se proponen varias acciones correctivas, entre ellas el desarrollo de la Apicultura como estrategia de gestión ambiental para la recuperación de la cuenca del embalse Guaremal.Palabras clave: Apicultura, Gestión ambiental, embalse Guaremal.","palabras_clave":"Apicultura|Gestión ambiental|embalse Guaremal"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64061,"idioma_id":4,"titulo":"Current Estimation for Pulsed Power Applications","resumen":"This paper presents a current sensing method to estimate the load current in pulsed current applications using a current transformer. The droop effect generated by this kind of sensor when measuring dc currents is compensated by means of signal processing techniques. The method is based on the inclusion of a sensor model in the system model so as to obtain an extended state-space model enabling to estimate the load current. A Kalman observer is employed in order to obtain an estimation with high noise rejection. The implementation of the observer in an FPGA platform allows to incorporate this block as a stage independent of the converter control. Experimental results validate the implementation of the proposed system.","palabras_clave":"PULSED POWER CONVERTER|CURRENT MEASUREMENT|FPGA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64062,"idioma_id":4,"titulo":"The variation of the fine structure constant: an update of statistical analysis with recent data","resumen":"We analyze different astronomical data of the variation in the fine structure constant obtained with KECK and VLT to check their consistency.We test consistency using the Student test and confidence intervals. We split the data sets in smaller intervals and group them by i) redshift and ii) angular position.&nbsp; Another statistical analysis is proposed considering phenomenological models for the variation in $alpha$.Results show consistency for reduced intervals for each pair of data sets considered and suggest that the variation in $alpha$ is important at higher redshifts.Even though the ``dipole model´´ proposed by Webb et al. seems to be the most accurate phenomenological model, the statistical analyses indicates that the variation in $alpha$ might be depending both on redshift and angular position.","palabras_clave":"quasars|absorption lines|cosmology|miscellaneous"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64063,"idioma_id":4,"titulo":"MicroRNA miR396 and RDR6 synergistically regulate leaf development","resumen":"The microRNA (miRNA) miR396 regulates GROWTH-REGULATING FACTORs (GRFs), a plant specific family of transcription factors. Overexpression of miR396 causes a decrease in the GRFs that has been shown to affect cell proliferation in the meristem and developing leaves. To bring further insights into the function of the miR396 regulatory network we per- formed a mutant enhancer screen of a stable Arabidopsis transgenic line expressing 35S:miR396b, which has a reduction in leaf size. From this screen we recovered several mutants enhancing this phenotype and displaying organs with lotus- or needle-like shape. Analysis of these plants revealed mutations in as2 and rdr6. While 35S:miR396b in an as2 context generated organs with lotus-like shape, the overexpression of the miRNA in an rdr6 mutant background caused more important developmental defects, including pin-like organs and lobed leaves. Combination of miR396 overexpressors, and rdr6 and as2 mutants show additional organ defects, suggesting that the three pathways act in concert. Genetic interactions during leaf development were observed in a similar way between miR396 over- expression and mutants in RDR6, SGS3 or AGO7, which are known to participate in trans- acting siRNA (ta-siRNA) biogenesis. Furthermore, we found that miR396 can cause lotus- and pin-like organs per se, once a certain expression threshold is overcome. In good agree- ment, mutants accumulating high levels of TCP4, which induces miR396, interacted with the AS1\/AS2 pathway to generate lotus-like organs. The results indicate that the miR396 regulatory network and the ta-siRNA biogenesis pathway synergistically interact during leaf development and morphogenesis.  &#57346;","palabras_clave":"MicroRNA|MiR396|ta-siRNA|GRF"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64064,"idioma_id":4,"titulo":"miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula","resumen":"The root system is crucial for acquisition of resources from the soil. In legumes, the efficiency of mineraland water uptake by the roots may be reinforced due to establishment of symbiotic relationships withmycorrhizal fungi and interactions with soil rhizobia. Here, we investigated the role of miR396 in regulatingthe architecture of the root system and in symbiotic interactions in the model legume Medicago truncatula.Analyses with promoter\u2013GUS fusions suggested that the mtr-miR396a and miR396b genes are highlyexpressed in root tips, preferentially in the transition zone, and display distinct expression profiles duringlateral root and nodule development. Transgenic roots of composite plants that over-express the miR396bprecursor showed lower expression of six growth-regulating factor genes (MtGRF) and two bHLH79-liketarget genes, as well as reduced growth and mycorrhizal associations. miR396 inactivation by mimicrycaused contrasting tendencies, with increased target expression, higher root biomass and more efficientcolonization by arbuscular mycorrhizal fungi. In contrast to MtbHLH79, repression of three GRF targets byRNA interference severely impaired root growth. Early activation of mtr-miR396b, concomitant with posttranscriptionalrepression of MtGRF5 expression, was also observed in response to exogenous brassinosteroids.Growth limitation in miR396 over-expressing roots correlated with a reduction in cell-cycle geneexpression and the number of dividing cells in the root apical meristem. These results link the miR396network to the regulation of root growth and mycorrhizal associations in plants.","palabras_clave":"microRNA,|Medicago truncatula|growth regulating factor|root apical meristem"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64065,"idioma_id":4,"titulo":"Late Miocene to recent morphotectonic evolution and potential seismic hazard of the northern Lerma valley: clues from Lomas de Medeiros, Cordillera Oriental, NW Argentina","resumen":"The Lomas de Medeiros are lowrelief hills located at the northern extreme of the Lerma valley,where newstructural,morphological and topographic key data have been collected and integrated with the available stratigraphic  and geochronological data in order to elucidate the morphotectonic evolution of the region during the last ~10 Ma. In this area, Pliocene to Lower Pleistocene conglomerates and siltstones (Piquete Formation) are deformed delineating an asymmetrical anticline with eastward vergence, axial plane trending N10°E, and progressive unconformities at the backlimb. Syntectonic sedimentation has been documented as related toN?S trending normal faulting near the fold axis as well. Middle Pleistocene to Holocene thick fluvial conglomerates cover unconformably the Piquete Formation as strath terraces with a few meters of thickness. The higher and oldest terrace has been uplifted more than 160 m respect to the top of Calvimonte Formation conglomerates (~0.33 Ma), its stratigraphic correlate at the undeformed Lerma valley. Detailed topographic profiles of the terraces showevidences of progressive uplifting by folding and the presence of scarps relatedwith reverse faulting. The anticline has been modeled using the fault propagation folding mechanism with a blind ramp dipping 32° to the west and a decollement level located 2.7 km below the surface, at the contact between the Cenozoic cover and the Paleozoic basement. The integration of these observationswith previously published data allowed to establish the morphotectonic evolution of this region during the last 10 Ma. Folded and faultedMiddle Pleistocene  to Holocene terraces at Lomas deMedeiros and Upper Pleistocene deposits uplifted at Mojotoro range are strong evidences of the seismogenic potential of the faults related with these morphostructures. Applying relationships with the subsurface rupture length, earthquakes with maximum moment magnitudes (Mw) of 6.5, 7.0 and 7.1 have been estimated for Medeiros, Lesser and Mojotoro faults respectively.","palabras_clave":"NEOTECTONICS|TECTONIC GEOMORPHOLOGY|PROGRESSIVE UNCONFORMITIES|SYNTECTONIC SEDIMENTATION|FOLDED TERRACES|SEISMIC HAZARD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64066,"idioma_id":4,"titulo":"Smooth null hypersurfaces near the horizon in the presence of tails","resumen":"We show that the power-law decay modes found in linear perturbations of   Schwarzschild black holes, generally called tails, do not produce   caustics on a naturally defined family of null surfaces in the   neighborhood of i+ of a black hole horizon.","palabras_clave":"Relatividad General|Black holes|tails"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64067,"idioma_id":4,"titulo":"Digital Control for a Multiple-Stage Pulsed Current  Source","resumen":"Abstract?Multiple-stage converters are composed of a set of  structures with different voltage, current and switching frequency  capabilities. In particular, in applications like particle accelerator  beam deflection and focusing, the requirement is high-current  high-precision current pulses generation. In order to satisfy the  specifications of this application, a novel multiple-stage converter  topology was proposed and presented in a previous work.  However, said proposal should be complemented with a control  system to perform the control of each structure, manage its global  interconnection and execute the regulation loops. This work  describes the implementation of the digital control developed  for the proposed converter. Experimental results are presented  by applying the proposed control to a prototype.","palabras_clave":"Digital Control|Pulsed Power Converter|Power Electronics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64068,"idioma_id":4,"titulo":"Multiple RNA recognition patterns during microRNA biogenesis in plants","resumen":"MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal miRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable size and shape. Here, we design an approach to systematically analyze miRNA processing intermediates and characterize the biogenesis of most of the evolutionarily conserved miRNAs present in Arabidopsis thaliana. We found that plant miRNAs are processed by four mechanisms, depending on the sequential direction of the processing machinery and the number of cuts required to release the miRNA. Classification of the precursors according to their processing mechanism revealed specific structural determinants for each group. We found that the complexity of the miRNA processing pathways occurs in both ancient and evolutionarily young sequences and that members of the same family can be processed in different ways. We observed that different structural determinants compete for the processing machinery and that alternative miRNAs can be generated from a single precursor. The results provide an explanation for the structural diversity of miRNA precursors in plants and new insights toward the understanding of the biogenesis of small RNAs.","palabras_clave":"microRNA|Biogenesis|plant"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64069,"idioma_id":4,"titulo":"Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors ROR&#947;t and Ahr that leads to IL-17 production by activated B cells","resumen":"  Here we identified B cells as a major source of rapid,   innate-like production of interleukin 17 (IL-17) in vivo in response to   infection with Trypanosoma cruzi. IL-17(+) B cells had a plasmablast   phenotype, outnumbered cells of the TH17 subset of helper T cells and   were required for an optimal response to this pathogen. With both mouse   and human primary B cells, we found that exposure to parasite-derived   trans-sialidase in vitro was sufficient to trigger modification of the   cell-surface mucin CD45, which led to signaling dependent on the kinase   Btk and production of IL-17A or IL-17F via a transcriptional program   independent of the transcription factors ROR&#947;t and Ahr. Our combined   data suggest that the generation of IL-17(+) B cells may be a previously   unappreciated feature of innate immune responses required for pathogen   control or IL-17-mediated autoimmunity.","palabras_clave":"VIrulence factors|Chagas disease|Glycobiology of the immune system|sialic acids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64070,"idioma_id":4,"titulo":"Vitamin D Synthesis Measured With A Multiband Filter Radiometer In Río Gallegos, Argentina","resumen":"Vitamin D plays an important role in human health. Vitamin D production from the sun is affected by UVB solar radiation. This paper presents a simple method for retrieving vitamin D-weighted UV by using a multiband filter radiometer GUV-541 installed at the Atmospheric Observatory of Southern Patagonia (OAPA) (51 ° 33' S, 69° 19' W), Río Gallegos. The methodology used combines irradiance measurements from a multiband filter radiometer with spectral irradiance modeled by the SOS radiative transfer code (developed by Lille University of Science and Technology (USTL)). The spectrum modeled is weighted with vitamin D action spectra published by the International Commission on Illumination (CIE), which describes the relative effectiveness of different wavelengths in the generation of this particular biological response. This method is validated using the vitamin D-weighted UV derived from a Brewer MKIII spectrophotometer (SN 124) belonging to the National Institute for Spatial Research (INPE), Brazil, which is able to measure solar spectra between 290 and 325nm. The method presents a good correlation between the two independent instruments. This procedure increases the instrumental capabilities of the multiband filter radiometer. Moreover, it evaluates the annual variation of vitamin D-weighted UV doses from exposure to ultraviolet radiation. These values are likely to be lower than suitable levels of vitamin D during winter and part of spring and autumn at these latitudes.","palabras_clave":"vitamin D|UV radiation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64071,"idioma_id":4,"titulo":"Study Of Cloud Enhanced Surface UV Radiation At Atmospheric Observatory Of Southern Patagonia, Río Gallegos, Argentina.","resumen":"Ozone and ultraviolet (UV) radiation are two important issues in the study of Earth\u2019s atmosphere. The anthropogenic perturbation of the ozone layer has induced change in the amount of UV radiation that reaches the Earth\u2019s surface, mainly through the Antarctic ozone hole. Also clouds have been identified as the main modulator of UV amount over short time scales. While clouds can decrease direct radiation, they can produce an increase in the diffuse component, and as a consequence the surface UV radiation may be higher than during an equivalent clear sky scenario. Inparticular this situation can be important when a low ozone column and partially cloud coverered skies occur simultaneously. These situations happen frequently in southern Patagonia, where the CEILAP Lidar Division has established the Atmospheric Observatory of Southern Patagonia, an atmospheric remote sensing site near the city of Río Gallegos (51º55\u2019S, 69º14\u2019W). In this paper, the impact of clouds on UV radiation is investigated by the use of ground based measurements from the passive remote sensing instruments operating at this site, mainly broad and moderate narrow band filter radiometers. Cloud modification factors (CMF, ratio between the measured UV radiation in a cloudy sky and the simulated radiation under cloud-free conditions) are evaluated for the study site. CMFs higher than 1 are found during spring and summer time, when lower total ozone columns, higher solar elevations and high cloud coveroccur simultaneously, producing extreme erythemal irradiance at the ground surface. Enhancements as high as 25% were registered. The maximum duration of the enhancement was around 30 minutes. This produces dangerous sunbathing conditions for the Río Gallegos citizen.","palabras_clave":"UVI|CMF|Ozone|Remote sensing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64072,"idioma_id":4,"titulo":"Microstructure and corrosion behaviour of AISI 316L duplex treated by means of ion nitriding and plasma based ion implantation and deposition","resumen":"Austenitic stainless steels are the preferred materials for mechanical components in the food, oil and chemical industries due to their good corrosion resistance but they often suffer severe wear due to their poor tribological properties. Plasma surface engineering has been extended to stainless steel hardening, through the modification of the surface with ion nitriding or the application of hard coatings.    In this work, AISI 316L samples were coated with titanium nitride by means of a cathodic arc (CA) and this technique was combined with plasma immersion ion implantation, in a process called plasma based ion implantation and deposition (PBII&D). The effect on corrosion resistance was analyzed, also considering ion nitriding as a pre-treatment. Microstructure and composition were analyzed by means of glancing angle XRD, SIMS, SEM, SEM-FIB and EDS. Localized corrosion resistance was analyzed in a cyclic potentiodynamic polarization experiment in 3.5% NaCl solution, and the corroded surface was analyzed with AFM, SEM and EDS as well. It was found that nitriding at 400 °C produces a thin nitrided layer with good corrosion resistance. A duplex TiN coating resulted in a further improvement of the corrosion resistance. Comparing cathodic arc and PBII&D coating techniques, the last one gives the thickest film and also the best corrosion behavior with good adhesion.","palabras_clave":"Plasma nitriding|TiN coating|Plasma ion implantation|Corrosion resistance"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64073,"idioma_id":5,"titulo":"Regeneración de plantas de té (Camellia sinensis) por cultivo in vitro de meristemas, yemas axilares y segmentos uninodales","resumen":"Tres tipos de explantes de dos clones( C H 1 4 I N TA y C H 3 1 8 I N TA ) d e t é(Camellia sinensis (L.) O. Kuntze) fueronevaluados para su regeneración in vitro, bajola influencia de dos citocininas (BAP y CIN)y una giberelina (AG3). Previa desinfección,con etanol 70% (1 minuto) e hipoclorito desodio 1,5% (20 minutos) y tres enjuagues conagua destilada estéril, los explantes fueronaislados y cultivados en los distintos medios decultivo. Las mejores respuestas en formaciónde vástagos se registraron con los segmentosuninodales de ambos clones cultivados en elmedio ½ MS + 1 mg\/L de BAP o con el cultivode yemas axilares del clon CH 14 INTA enel medio ½ MS + 1 mg\/L de BAP o del clonCH 318 INTA en el medio ½ MS + 1 mg\/LBAP + 1 mg\/L AG3. Los mejores resultadoscon el empleo de meristemas caulinaresse obtuvieron en el medio ½ MS + 1 mg\/Lde CIN y 1 mg\/L de AG3. Los vástagosobtenidos fueron enraizados mediante sucultivo en ¼ MS + 6 mg\/L de IBA.","palabras_clave":"CAMELLIA SINENSIS|MERISTEMAS|MICROPROPAGACION|REGENERACION DE PLANTAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64074,"idioma_id":4,"titulo":"High Reliability and Performance PWM Inverter for Standalone Microgrids","resumen":"In this paper a high reliability and performance single-phase PWM inverter for standalone microgrids is presented. To obtain the before mentioned characteristics this work propose, two different controller structures and a straightforward design methodology for these controllers, based on the internal model principle. In order to validate the proposed design methodology, digital controllers and to demonstrate the steady state and transient performance, experimental results are presented from a 5kVA PWM modulated single phase inverter, fully controlled by a DSP TMS320F2407.","palabras_clave":"DC AC CONVERTER|DC DC CONVERTER|DIGITAL CONTROL|INTERNAL MODEL PRINCIPLE|MICROGRIDS|PHOTOVOLTAIC INVERTER|STANDLONE PHOTOVOLTAIC SYSTEMS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64075,"idioma_id":4,"titulo":"Competitive adsorption behavior of &#946;-lactoglobulin, &#945;-lactalbumin, bovin serum albumin in presence of             hydroxypropylmethylcellulose. Influence of pH","resumen":"aaa","palabras_clave":"BETA-LACTOGLOBULIN|ALPHA-LACTALBUMIN|BOVIN SERUM ALBUMIN|HYDROXYPROPYLMETHYLCELLULOSE|AIR\/WATER INTERFACE|PH-DEPENDENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64076,"idioma_id":4,"titulo":"SCHEDULING SCHEMES  WITH ADAPTIVE BLIND DETECTION FOR CODE REUSE IN MULTIUSER  MIMO SYSTEMS","resumen":"To improve the spectrum efficiency in wireless MIMO systems, it is&nbsp;&nbsp; essential to mitigate&nbsp;&nbsp;&nbsp;&nbsp; the channel interference. Opportunistic&nbsp;&nbsp; scheduling of multiusers and interference&nbsp;&nbsp;&nbsp;&nbsp; cancellation, such as&nbsp;&nbsp; adaptive multiuser detection, are two commonly used techniques.&nbsp;&nbsp;&nbsp;&nbsp; In&nbsp;&nbsp; this paper we study the performance improvement of using jointly these&nbsp;&nbsp; techniques&nbsp;&nbsp;&nbsp;&nbsp; for enabling the use of the same set of spreading codes&nbsp;&nbsp; to transmit two data streams&nbsp;&nbsp;&nbsp;&nbsp; in closed loop multiuser MIMO systems.&nbsp;&nbsp; We show that with the proposed scheduling schemes,&nbsp;&nbsp;&nbsp;&nbsp; the performance&nbsp;&nbsp; of an Adaptive Blind Receiver (ABR) is not degraded when the scheduled&nbsp; users share the same spreading code.","palabras_clave":"SCHEDULING|BLIND|CODE-REUSE|MIMO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64077,"idioma_id":4,"titulo":"Anti-inflammatory properties of phenolic lactones isolated from Caesalpinia paraguariensis stem bark","resumen":"Ethnopharmacological relevance: Caesalpinia paraguariensis (D. Parodi) Burkart stem bark infusion (CPBI) is traditionally used in Argentina because their ?vulnerary? properties. Aim of the study: CPBI was studied throughout bio-guided purification procedures conducted by in vitro biological assays in order to isolate the main bioactive compounds. Material and methods: Anti-inflammatory activity was assessed by enzyme inhibition assays of Hyaluronidase (Hyal) and inducible Nitric Oxide Synthase (iNOS). The antioxidant properties were evaluated by DPPH free radical scavenging assay, lipid peroxidation inhibition assay on erythrocyte membranes, and a cell-based assay that included the fluorescent probe (DCFH-DA) for indicating reactive oxygen species (ROS) generation. Bioactive compounds were purified by chromatographic methods and their structures elucidated using spectroscopic methods (ESI-MS and 1D\/2D-1H\/13C-NMR).  Results: Four main bioactive compounds were isolated from CPBI: ellagic acid (1), 3-O-methylellagic acid (2), 3,3´-di-O-methylellagic acid (3) and 3,3´-di-O-methylellagic-4-&#946;-D-xylopyranoside (4). These were bioactive at concentrations in which are present in CPBI, being compounds 2 and 3 the best enzyme inhibitors of Hyal and iNOS, reaching the 90% inhibitory concentration (IC90) values ranging from 2.8 to 16.4 µM, that are better than that of the positive controls, aspirin (IC90: no reached) and aminoguanidine (IC90: 20.2 µM) respectively. Compounds 2 and 3 were also better scavengers for lipoperoxides than butylated hydroxytoluene (BHT), reaching the 90 % effective concentration (EC90) at 1.2-4.5 µg\/ml, and for DPPH radical (2.5-7.3 µg\/ml); moreover compounds were able to exert its scavenging action on intracellular ROS. Structural features relevant to the biological activities are discussed.  Conclusions: This work provides scientific validity to the popular usage of CPBI.","palabras_clave":"CEASALPINIA|ELLAGIC|ANTIOXIDANT|ANTIINFLAMMATORY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64078,"idioma_id":4,"titulo":"Dynamic Modeling and Parameter Estimation for Unit Operations in Lignocellulosic Bioethanol Production","resumen":"During the past decades, intensive research has been pursued on the development of kinetic models to predict  process behavior in ethanol production from lignocellulose. These models comprise a large number of parameters which have to  be tuned with appropriate experimental data. Therefore, the parameter estimation problem plays an essential role. This work  addresses the parameter estimation problem in models representing dilute acid hydrolysis, detoxification, and cofermentation  operations in the biochemical production of ethanol from lignocellulosic biomass. The models are represented by sets of  differential-algebraic equations (DAEs). Unlike previous approaches, these models account for the main process variables that  affect the entire process, specially the final production of bioethanol. These detailed kinetic models, systematically tuned with  experimental data, can be used in future studies within a model-based framework that allows performing realistic simulation and  optimization aimed at bioethanol process design. A sensitivity analysis has been performed in order to identify the most sensitive  parameters. The parameter estimation problem is solved with a simultaneous optimization approach in which the system of  dynamic equations is converted into a set of algebraic ones through orthogonal collocation on finite elements. Thus, estimating  the model parameters entails optimizing a weighted least squares objective function subject to the discretized algebraic  constraints, resulting in a large-scale nonlinear programming problem (NLP). A good agreement with available experimental data  has been obtained with estimated kinetic parameters in each model.","palabras_clave":"Bioethanol|Parameter estimation|Kinetic models|Lignocellulosic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64079,"idioma_id":4,"titulo":"Impact of ciprofloxacin and chloramphenicol on the lipid bilayer of Staphylococcus aureus. Changes in membrane potential","resumen":"The present study was undertaken to explore the interaction of ciprofloxacin and chloramphenicol with bacterial membranes ina sensitive and in a resistant strains of Staphylococcus aureus by using 1-anilino-8-naphthalene sulfonate (ANS). The binding ofthis probe to the cell membrane depends on the surface potential, which modulates the binding constant to the membrane. Weobserved that these antibiotics interacted with the bilayer, thus affecting the electrostatic surface potential. Alterations caused byantibiotics on the surface of the bacteria were accompanied by a reduction in the number of binding sites and an increase in theANS dissociation constant in the sensitive strain, whereas in the ciprofloxacin-resistant strain no significant changes were detected.Thechanges seen in the electrostatic surface potential generated in themembrane of S. aureus by the antibiotics provide new aspectsconcerning their action on the bacterial cell.","palabras_clave":"STAPHYLOCOCCUS AUREUS|MEMBRANE POTENTIAL|OXIDATIVE STRESS|ANTIBIOTIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64080,"idioma_id":5,"titulo":"Jardines Maternales: Los primeros pasos en el proceso de separación","resumen":"Frecuentemente los Jardines Maternales comparten con la familia, los cuidados de bebés. Esta realidad nos lleva a preguntarnos ¿Resulta precoz la concurrencia de bebés a instituciones educativas antes de los tres meses? y ¿cuál es su función durante los primeros años de vida?.   Para delinear respuestas presentamos un recorte de resultados de investigación, focalizado en la dimensión relacional del proceso de separación, de bebés menores de tres meses.   La recolección del material se realizó mediante el Método de Observación de Lactantes (Bick, 1948) y la filmación de las observaciones, luego se construyeron categorías de análisis.   En concordancia con los desarrollos teóricos vigentes y el material empírico, consideramos que las intervenciones deben girar en torno a la legislación laboral, los aspectos organizacionales y las modalidades vinculares.","palabras_clave":"JARDINES MATERNALES|CONDICIONES AMBIENTALES|BEBÉS|VÍNCULOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64081,"idioma_id":5,"titulo":"Aspectos biológicos de Acacia longifolia, una especie exótica potencialmente invasora de pastizales psamófilos","resumen":"El sistema de dunas costeras de la Pampa Austral constituye un ambiente de suma importancia por mantener valiosas funciones ecosistémicas y conservar una alta biodiversidad. Acacia longifolia (aromo) es un árbol exótico establecido y\/o invasor en regiones costeras de nuestro país. Este trabajo describe un núcleo poblacional de la especie que crece sobre dunas litorales de la localidad de Monte Hermoso (Buenos Aires) y evalúa aspectos de la biología de sus semillas, incluyendo su peso y tamaño, tasa de germinación con y sin tratamientos de escarificación e intensidad de la remoción por predadores en áreas de pastizal prístinos. El núcleo exhibe una densidad media de 510 (ES=192,8) individuos\/ha, con una cobertura de la canopia promedio de 42,85 % (ES=6,19) y una variación de 6,45-14,83 cm en el diámetro de sus troncos. Las semillas de aromo miden en promedio 3,8±0,07 mm de largo y 2,4±0,04 mm de ancho, con un peso medio de 16,08±0,4 mg. El porcentaje de germinación resultó máximo previa escarificación química o física de las semillas (90±2,98 % y 84±5,62 %, respectivamente). La tasa de remoción de las semillas resultó del 27 % (ES=8,3). Los resultados obtenidos aportan información sobre aspectos ecológicos de una especie leñosa exótica presente en pastizales pampeanos. Sus antecedentes como invasora en otros ambientes similares postulan a la especie como riesgosa desde el punto de vista de la conservación. La especie estaría establecida en las dunas de Monte Hermoso, lo que resulta importante monitorear su estado y evitar que traspase la barrera de dispersión.","palabras_clave":"Dunas costeras|Ecología de semillas|plantas invasoras"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64082,"idioma_id":4,"titulo":"Numerical and experimental comparison of an all-fiber APM laser with two-coupled linear cavities","resumen":"In this work, some properties of a two coupled cavities all-fiber laser as a function of the ratio between their optical lengths are analyzed. The laser is composed of an active primary cavity generated by an erbium-doped fiber and two fiber Bragg gratings (FBG1 and FBG2 ), and a passive cavity made with an appropriate length of standard optical fiber spliced to FBG2 and employing the Fresnel reflection at the other end of the pigtail. The operation of this additive-pulse mode-locking (APM) laser is based on the coherent addition of pulses that interact on a central reflective element which is a common component for both cavities. The developed model considers the propagation of electric fields into the system solving the non-linear Schrödinger equation (NLSE) through the Split-Step Method (SSM). Reflectivity and transmittance characteristics of the FBGs used as feedback elements are simulated by using the Transfer-Matrix Method (TMM). The main system parameters employed on the calculations are: group velocity dispersion (GVD), self-phase modulation (SPM), gain factor that includes the effects of self-saturation, fiber losses and dispersion of the FBGs. Numerical results for the pulse width and the peak power of the output, obtained from the application of the numerical model, are compared with experimental values measured by varying the length of the auxiliary cavity. A good agreement between them is observed. When the auxiliary cavity length is reduced with respect to the main cavity length, both experimental and numerical results show a decrease in the temporal width with a corresponding increase in the repetition frequency of the pulses. If the main cavity length is made two or three times larger than the original one by varying the pigtail length, some of the laser output frequencies previously obtained are also founded. In that cases, similar values of the pulse width were found. This pulsewidth dependence with the repetition frequency was also verified by applying our mathematical model.","palabras_clave":"Fiber lasers|Modulation tunning and mode locking|Propagation scattering and losses|Gratings"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64083,"idioma_id":4,"titulo":"PTP1B promotes focal complex maturation, lamellar persistence and directional migration","resumen":"Previous findings established that ER-bound PTP1B targets peripheral cell-matrix adhesions and regulates positively cell adhesion to fibronectin. Here we show that PTP1B enhances focal complex lifetime at the lamellipodium base, delaying their turnover and facilitating &#61537;-actinin incorporation. We demonstrate the presence of catalytic PTP1BD181A-&#61537;-actinin complexes at focal complexes. Kymograph analysis reveals that PTP1B contributes to lamellar protrusion persistence and directional cell migration. Pull down and FRET analysis also shows that PTP1B is required for efficient integrin-dependent downregulation of RhoA and upregulation of Rac1 during spreading. A substrate trap strategy revealed that FAK\/Src recruitment and Src activity were essential for the generation of PTP1B substrates in adhesions. PTP1B targets the negative regulatory site of Src (phosphotyrosine 529), paxillin and p130Cas at peripheral cell-matrix adhesions. We postulate that PTP1B modulates more than one pathway required for focal complex maturation and membrane protrusion, including &#61537;-actinin-mediated cytoskeletal anchorage, integrin-dependent activation of the FAK\/Src signaling pathway, and RhoA and Rac1 GTPase activity. By doing so, PTP1B contributes to coordinate adhesion turnover, lamellar stability and directional cell migration.","palabras_clave":"ptp 1b|src|fak|migration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64084,"idioma_id":4,"titulo":"Picomolar gradients of progesterone select functional human sperm even in subfertile samples","resumen":"More than 1 million infertility treatments are practiced around the world per year, but only 30% of the couples succeed in taking ababy home. Reproductive technology depends in part on sperm quality, which influences not only fertilization but also embryo development andimplantation. In order to provide a better quality sperm subpopulation, innovative sperm selection techniques based on physiological sperm featuresare needed. Spermatozoa at an optimum state may be selected by following an increasing concentration gradient of picomolar progesterone,a steroid secreted by the cumulus cells at the time of ovulation. In this study we developed a method to recruit spermatozoa at the bestfunctional state, based on sperm guidance toward progesterone. The sperm selection assay (SSA) consists of a device with two wells connectedby a tube.Onewellwas filled with the sperm suspension and the other with picomolar progesterone, which diffused inside the connecting tube as agradient. The sperm quality after the SSA was analyzed in normal and subfertile semen samples. Several sperm parameters indicative of spermphysiological state were determined before and after the SSA: capacitation, DNA integrity and oxidative stress. After the SSA, the mean level ofcapacitated spermatozoa increased three times in normal and in subfertile samples. The level of sperm with intact DNA was significantlyincreased, while sperm oxidative stress was decreased after sperm selection. Interestingly, the exposure to a progesterone gradient stimulatedthe completion of capacitation in some spermatozoa that could not do it by themselves. Thus, the SSA supplies a sperm population enriched withspermatozoa at an optimum physiological state that may improve the assisted reproductive technology outcome.","palabras_clave":"espermtozoides|progesterona|seleccion espermatica|quimiotaxis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64085,"idioma_id":4,"titulo":"Recent patents for detecting the species of origin in animal feedstuff, and raw and processed meat products","resumen":"The value of the traceability and labeling of food is attributable to   two main aspects: health safety and\/or product or process certification.   The identification of the species related to meat production is still a   major concern for economic, religious and health reasons. Many   approaches and technologies have been used for species identification in   animal feedstuff and food. The early methods for meat products   identification include physical, anatomical, histological and chemical.   Since 1970, a variety of methods were developed, these include   electrophoresis (i.e. isoelectrofocusing), chromatography (i.e. HPLC),   immunological techniques (i.e. ELISA), Nuclear Magnetic Resonance, Mass   Spectrometry and PCR (DNA and RNA based methods). The recent patents on   species detection in animal feedstuffs, raw meat and meat processed   products, listed in this work, are mainly based on monoclonal antibodies   and PCR, especially RT-PCR. The new developments under research are   looking for more sensible, specific, less time consuming and   quantitatively detection methods, which can be used in highly processed   or heated treated meat food.","palabras_clave":"Contaminant|fraud|meat|processed|species identification|DNA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64086,"idioma_id":4,"titulo":"Longitudinal analysis of the effects of IGF1-SnaBI genotypes on the growth curve of Angus bull calves","resumen":"The Insulin Growth Factor 1 (IGF1) has been proposed as a candidate gene for growth related traits as it plays a central role in growth and development of mammals. A relationship between serum IGF1 concentration and different growth traits in cattle has been documented. Furthermore, the IGF1-SnaBI SNP in the gene promoter region influences gene expression and IGF1 blood level. The current research was conducted to perform a genetic analysis of longitudinal data for growth curves through the evaluation of the influence of IGF1-SnaBI genotypes on the growth curve of beef cattle during highly anabolic stages. Data were taken on 275 Angus bull calves on two consecutive years, in two commercial cow?calf operations. Calves were weighed at birth, at weaning, and at 3 to 4 times before the year of age. A random regression animal model was employed for the analysis. Fixed effects were age of dam, herd-year, and age of calf (linear and quadratic terms nested within IGF1-SnaBI genotype). Random effects were age at measure (linear and a quadratic terms nested within animal). The analysis showed significant differences (P<0.01) in the growth curves at ages in between 66 and 291 days for the IGF1-SnaBI genotypes. At 210 days of age, the effect of substituting a T by a C was estimated to be 5.21kg. These results suggest that the effect of IGF1-SnaBI over growth in cattle take place mostly before puberty.","palabras_clave":"insulin Growth Factor 1|Growth|Cattle|Bull calves"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64087,"idioma_id":5,"titulo":"Publicaciones especializadas y liberalismo periférico. Notas preliminares sobre la transmisión de ideas económicas en Argentina 1870-1890","resumen":"Este trabajo se inserta en un proyecto mayor que explora la articulación entre la conformación de 'saberes de Estado' -en particular la economía-, la modernización del Estado, la circulación transnacional de ideas, y los momentos definidos socialmente como críticos en la Argentina. El objetivo que nos planteamos en este artículo es analizar una serie de publicaciones que operaron como 'correas de transmisión' del saber económico y, en términos más generales, de concepciones vinculadas al liberalismo durante las últimas décadas del siglo XIX. Lo que pretendemos reconstruir parcialmente a partir de éste y otros trabajos es el doble proceso a partir del cual el saber sobre la economía da lugar a un 'discurso experto' reconocido socialmente como tal y, al mismo tiempo, ciertas ideas vinculadas a la economía y a la sociedad llegaron a conformar lo que podría definirse como un ?siempre cambiante- ?sentido común social?. Por ?sentido común social? entendemos la forma en que ciertos sistemas de ideas y de creencias trascienden los ámbitos académicos y, en general, aquellos vinculados a la 'alta cultura' para conformar aquella porción de la realidad que Peter Berger definió como 'el mundo como dado', es decir lo que está más allá de cuestionamientos inmediatos.   \tEn un trabajo anterior focalizamos en la difusión de ideas sobre economía en el mundo académico . En este texto, que puede ser leído como complementario del anterior, comenzamos a mapear circuitos de recepción y circulación de ideas económicas y sociales que pasaban por fuera de los 'grandes periódicos' y los 'grandes pensadores'. Focalizaremos en tres publicaciones cuyo interés reside no solamente en los grupos a los que representan, sino en el hecho que podrían ser descriptas como 'heterodoxas' en lo que se refiere a sus posturas respecto del debate proteccionismo-librecambio, de la política económica en general, y de la definición de las responsabilidades que atribuían al Estado. Los periódicos son: El Industrial, publicado por el Club Industrial creado en 1875; El Economista, publicado a partir de 1877, y la Revista Económica del Río de la Plata (RERP) que tuvo dos épocas: la primera ?muy breve- a principios de los 70, y una posterior mucho más extensa que comienza en 1892.   Estas tres publicaciones respondían a intereses particulares y formaban parte de una constelación de publicaciones especializadas existente desde las últimas décadas del siglo XIX en la Argentina. Estos periódicos, además, conformaban un verdadero espacio de sociabilidad en el que se establecían diálogos y debates, se traducían y reproducían textos extranjeros, y desde donde se interpelaba al Estado. En este sentido formaban parte de una incipiente aunque muy activa 'esfera pública', es decir, núcleos de presión, crítica y diálogo con los poderes públicos. Así, por ejemplo, en abril de 1876, representantes de 16 diarios y periódicos de todas las tendencias se pusieron de acuerdo (más por consenso que por unanimidad y a pesar de las críticas puntuales de algunos de ellos) para proponer un proyecto de ley provincial sobre cédulas hipotecarias y enajenación de obras de salubridad. Entre los periódicos participantes estaban los 'grandes' como La Nación, pero también los 'pequeños' como El Industrial que sostenían posiciones opuestas sobre muchos temas. En general, la historiografía ha prestado atención a los 'grandes periódicos' o a la prensa partidaria en los análisis de conformación de una esfera pública a partir de la segunda mitad del siglo XIX . Sin embargo, la existencia de 'pequeños periódicos' de frecuencia semanal o quincenal, que respondía a intereses corporativos, profesionales o técnicos, con diverso nivel de sofisticación doctrinaria, también constituyó un elemento importante de debate e intervención en la cosa pública, y sobre algunos de ellos nos detendremos en este trabajo. Estos periódicos se definían a sí mismos por estar ubicados por fuera (y en oposición) a la política partidaria. Cada uno de ellos decía defender posiciones 'concretas' y 'realistas' para el bien del país frente a la prensa partidaria y a la política en general. Todos ellos participaban, cada uno a su manera, del 'consenso liberal' que se estableció en el país durante la segunda mitad del siglo XIX, pero a su vez introducían en él importantes matices que dan cuenta de patrones particulares de recepción de las ideas liberales en Argentina y América Latina durante el siglo XIX. Nos centraremos en un período particular delimitado por dos crisis: la ocurrida en la década de 1870 y la de 1890, partiendo de la hipótesis de que las crisis constituyen 'momentos fuertes' en el proceso de construcción y consolidación de los saberes de Estado, que abrirían las puertas para cambiar el universo de lo decible permitiendo cambios de paradigmas.","palabras_clave":"LIBERALISMO|INDUSTRIALES|PROTECCION ADUANERA|CIRCULACION DE SABERES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64088,"idioma_id":5,"titulo":"Aportes de la dendrocronología al estudio de la evolución del caldenar pampeano durante la ocupación ranquelina","resumen":"El análisis distribucional de las ocupaciones ranquelinas en el norte de la pampa seca indica que la zona de bosques del caldenal pampeano habría sido el ambiente de mayor atractivo para la instalación de los cacicazgos. En tal sentido resulta de interés efectuar un estudio sobre el impacto que habrían tenido las estrategias de subsistencia de los ranqueles sobre el monte de caldén que ellos ocuparon. En este trabajo presentamos algunos aportes efectuados desde la dendrocronología para la identificación de tales cambios, interrelacionando la información obtenida con los datos del registro arqueológico y las fuentes documentales. El caldén (Prosopis caldenia) es potencialmente favorable para efectuar estudios dendroecológicos ya que su crecimiento radial es sensible a eventos tales como cambios en la densidad forestal, el fuego y los parámetros edáficos de los sitios donde se extraen las muestras. Con tal objetivo se analizaron 38 piezas de esta especie extraídas de un sector del caldenar ubicado dentro del territorio que hasta 1879 controlaba el cacique ranquel Baigorrita. Dado que la mayor antigüedad de los anillos de crecimiento se remonta a 1750 -momento en que se consolidan los cacicazgos ranquelinos en la pampa seca-, resultó posible evaluar el impacto que las formas de subsistencia aborigen habrían producido en el monte, antes del frente de avance colonizador iniciado a fines del siglo XIX. Asimismo, el crecimiento heterogéneo de los anillos permite considerar la fluctuación climática entre años lluviosos y secos, aspectos que pueden correlacionarse con registros escritos y fotográficos.","palabras_clave":"RANQUELES|EVOLUCIÓN DEL CALDENAL|IMPACTO ANTRÓPICO|IMPACTO AMBIENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64089,"idioma_id":4,"titulo":"Regional patterns and controls of biomass in semiarid woodlands: lessons from the Northern Argentina Dry Chaco","resumen":"Land use change, particularly in forested ecosystems,has a direct impact on the global carbon cycle. Consequently, the regional assessment of biomass and the understanding of its current spatial controls are research priorities for regional ecology and land use. Field data and  satellite imagery were combined here to map woodlands and estimate their above-ground biomass (AGB) in the Dry Chaco ecoregion of northern Argentina. Allometric equations were used to derive AGB from diameter at breast height data collected at 50 samples during 2007. In order to  generate the AGB regional map, this information was later associated with MODIS-Terra spectral data (NDVI) using the Random Forest (RF) method. Finally, AGB spatial patterns were associated with potential biophysical and human controlling factors through correlation and regression analyses. Results indicate that the use of RF and NDVI of the dry season derived from MODIS-Terra was suitable to map regional AGB, what makes this methodology applicable to other dry woodlands. The RF model used to  map AGB showed a mean deviation of 2.9 % and a precision of 15 % for one prediction. At this regional scale of analysis, biophysical rather than human factors controlled AGB spatial patterns, in part because the region includes a wide range of environmental situations. Warmer conditions  showed a higher biomass, suggesting an energetic limitation for AGB accumulation. However, human controls (distance to towns, cultivation, and roads) also conditioned AGB patterns, suggesting lower AGB values near cultivated areas. The relation between AGB and water availability  was surprisingly weak, but partially obscured by the land use history and degradation due to extensive cattle ranching. We propose that a combination of environmental factor and land use affects the AGB regional patterns and promotes unexpected relationships with environmental factors. This work represents the first spatially explicit AGB (patterns and controls) analysis for an extensive subtropical dry woodland area (113,000 km2) and shows how biophysical and human factors co-control regional patterns.","palabras_clave":"Subtropical dry woodlands|Above-ground biomass|Forest degradation|Land use|Random Forest|Remote sensing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64090,"idioma_id":4,"titulo":"Detecting stationary gain changes in large process systems","resumen":"Stationary process gains are critical model parameters  to determine targets in commercial MPC technologies. Consequently, important  savings can be reached by acceding to an early prevention method capable of  detecting whether the actual process moves away from the modeled dynamics or  not, particularly by indicating when the process gains are not more represented  by those included in the model identified during commissioning stages. In this  first approach, a subspace identification method is used under open loop process  condition to develop a process gain-matrix estimator. The main reason for using  the subspace identification method is that it works directly with raw data and  that the development is intended for monitoring future applications under multivariable  closed-loop optimizing control where the transient regime is a frequent scenario. The objective of this paper is to present a method capable  of detecting those gains of a multivariable model that start moving away from  the original values. The anticipated knowledge of these events could provide a  warning to process engineers and prevent from targeting process conditions with  wrong gain estimations. The regular follow-up of the gain matrix should also help to localize those dynamics  needing an updating identification.","palabras_clave":"STEADY-STATE GAINS|MULTIVARIABLE ESTIMATOR|SUBSPACE IDENTIFICATION|LP-MPC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64091,"idioma_id":4,"titulo":"The influence of diet composition on egg and chick traits in Greater Rhea females under captivity","resumen":"1. This study was conducted to evaluate the influence of diet composition on egg number, physical and chemical characteristics of eggs and weight and survival of chicks throughout a breeding season in a captive-bred population of greater rheas (Rhea americana). 2. From August to December, individuals were offered two diets: processed feed for rheas and processed feed for chicken (which is the feed most commonly offered to farmed rheas in Argentina). Reproductive performance of 15 females was monitored and female body weight was recorded before egg-laying onset. Within each experimental group, the following variables were determined: egg morphometric variables and percentage of components, fatty acid composition, hatching success and initial weight of chicks and mortality during the first week of life. 3. Females that were fed on processed feed for rheas delayed onset of laying and reduced laying period and number of eggs produced. However, females of this group laid larger eggs, with higher percentages of yolk and yolk lipids, and exhibited higher hatching success and chick weight compared with those that received chicken diet. Survivorship of chicks in their first week of life was not affected by composition of the diet offered to parental female. 4. Some reproductive parameters of captive greater rhea females fed on processed feed for rheas were higher than those of individuals receiving processed feed for chicken.","palabras_clave":"ÑANDUES|RHEA|HUEVOS|PICHONES|DIETA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64092,"idioma_id":5,"titulo":"Por qué definir y clasificar las miocardiopatías","resumen":"Resumen no disponible","palabras_clave":"MIOCARDIOPATÍAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64093,"idioma_id":4,"titulo":"Age, growth and mortality in four populations  of the boring bivalve Lithophaga patagonica from Argentina","resumen":"                The boring bivalve Lithophaga patagonica (d´Orbigny, 1842) is a locally abundant inhabitant of hard  substrata in the coastal waters of the Southwestern Atlantic. In this paper we describe the growth, age and  mortality of three intertidal rock-boring populations of L. patagonica and one subtidal oyster shell (Ostrea puelcheana), boring population. An analysis of acetate peel replicas of shell sections showed that L. patagonica slows down its growth  during autumn-winter, which leads to changes in the direction and rate  of shell deposition and the formation of conspicuous annual (low temperature  induced) clefts in the shell margin. Cleft  counts and Von Bertalanffy growth analyses indicates a maximum lifespan of 15  years for L. patagonica, with noticeable  variations in longevity between populations (e.g., 6.5 years in the oyster  boring populations). Maximum asymptotic length (L&#8734;) and Von Bertalanffy growth constant (K) were  also variable between populations (L&#8734; between 14.76 and 36.95 mm and K from  0.20 to 0.90 yr-1 respectively). Mortality rates differed between sites. Type (rock vs. oyster),  composition and hardness of the substrata are likely the main factors controlling  the observed differences between populations.   ","palabras_clave":"AGE|GROWTH|LITHOPHAGA PATAGONICA|MORTALITY|OSTREA PUELCHEANA|ROCK BORING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64094,"idioma_id":5,"titulo":"Perfil cognitivo de niños con bajos rendimientos en comprensión lectora","resumen":"La mayoría de los niños con dificultades en la comprensión lectora poseen rendimientos inferiores en un conjunto de procesos cogniti-vos. El objetivo del presente estudio es establecer un perfil cognitivo asociado al bajo rendimiento en comprensión lectora en relación a la habilidad de monitoreo, a la producción de inferencias, al vocabulario, a la amplitud de memoria de trabajo y a la capacidad inhibitoria, en niños hispanoparlantes. Para ello se administraron tareas correspondientes a dichos procesos en niños con altos y bajos rendimientos en comprensión lectora. Los resultados mostraron que si bien los niños con mala comprensión presentan bajos rendimientos en estas habilidades, no puede establecerse un perfil consistente ya que hay niños con problemas en comprensión que presentan normal o superior rendimiento en algunas de estas habilidades cognitivas","palabras_clave":"Monitoreo|Inferencias|Vocabulario|Memoria de Trabajo|Inhibición"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64095,"idioma_id":5,"titulo":"Procesos gravitacionales ? fluviales y preservación de vegetación boscosa en un área volcánica: Formación Rancahu é (Oligoceno), Andes neuquinos, Argentina","resumen":"Depósitos clásticos de la Formación Rancahué (Oligoceno Superior) que constituye parte del registro volcánico y sedimentario de la cuenca oligocena-neógena de Aluminé (Cordillera de los Andes, 70º W y 39º S) permitieron analizar los fenómenos de interacción sedimentaria en ambientes volcánicos continentales y caracterizar los mecanismos de preservación de restos de troncos asociados a sucesiones aluviales volcano-sedimentarias. En estos depósitos, se ha identificado un bosque petrificado de tierras altas con 33 ejemplares preservados en posición de vida, con Nothofagoxylon como elemento dominante,  desarrollado bajo condiciones climáticas templadas y húmedas. En el área de estudio la Formación Rancahué se caracteriza por la estrecha interrelación lateral y secuencial entre tres asociaciones de facies. La asociación A se compone de  aglomerados y conglomerados gruesos monomícticos (con fenoclastos de basaltos) acumulados en ambientes proximales de altas pendientes (taludes y en el pie de taludes subaéreos) como producto de debris floods o de flujos hiperconcentrados, avalanchas,  deslizamientos y flujos de detritos. Estos depósitos tienen procedencia local y se vinculan con un cuerpo volcánico basáltico que generó un importante relieve intracuencal. La asociación B consiste en conglomerados, sabulitas, areniscas conglomerádicas y areniscas gruesas y muy gruesas con variadas estructuras primarias y se asigna a un sistema fluvial con desarrollo regional,  baja canalización y carga traccional, con áreas de aporte en la Cordillera de los Andes. La asociación C se compone de facies clásticas de textura fina acumuladas esencialmente por decantación en ambientes subaéreos y subácueos, con el periódico aporte de lluvias de cenizas volcánicas, y con niveles en los que se aprecia la formación de suelos. Los depósitos fluviales incluyen restos de troncos transportados que muestran el poder erosivo de las corrientes de flujo rápido que los acumularon. El bosque petrificado arraigó en depósitos de la asociación de facies C y quedó preservado en los suprayacentes aglomerados y conglomerados de la asociación A. Se interpreta que los troncos fueron sepultados por los flujos gravitacionales que operaron en abanicos aluviales y coluviales de la zona riparia hidrogeomórfica y que se caracterizan por una tasa de sedimentación alta y un discreto poder erosivo por su naturaleza laminar o quasi laminar.","palabras_clave":"Depósitos gravitacionales de talud|Depósitos fluviales proximales|Oligoceno|Andes neuquinos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64096,"idioma_id":4,"titulo":"Therapeutic potential of histamine H(4) receptor agonists in triple-negative human breast cancer experimental model.","resumen":"Background and purpose. The presence of the histamine H4 receptor (H4R) was   previously reported in benign and malignant lesions and cell lines derived from   the human mammary gland. The aim of this work was to evaluate the effects of H4R   ligands on the survival, tumour growth rate and metastatic capacity in a breast   cancer experimental model. Experimental approach. Xenograft tumours of the   highly invasive human breast cancer cell line MDA-MB-231 were established in   immune deficient nude mice. The following H4R agonists were employed: histamine   (5 mg?kg-1), clozapine (1 mg?kg-1), and the experimental compound JNJ28610244   (10 mg?kg-1). Results. Data indicate that developed tumours were highly   undifferentiated, expressed H4R and exhibited high levels of histamine content   and proliferation marker (PCNA) while displayed low apoptosis. Mice of the   untreated group displayed a median survival of 60 days, and a tumour doubling   time of 7.4±0.6 days. A significant decrease in tumour growth evidenced by an   augment of the tumour doubling time was observed in the H4R agonist groups   (13.1±1.2, P<0.01 in histamine group; 15.1±1.1, P<0.001 in clozapine   group; 10.8±0.7, P<0.01 in JNJ28610244 group). This effect was associated   with a decrease in the PCNA expression levels, and also a reduced intratumoural   vessels in histamine and clozapine treated mice. Histamine significantly   increased median survival (78 days; Log-rank Mantel-Cox Test, P=0.0025;   Gehan-Breslow-Wilcoxon Test, P=0.0158) and tumoural apoptosis. Conclusions and   Implications. Histamine through the H4R exhibits a crucial role in tumour   progression. Therefore, H4R ligands offer a novel therapeutic potential as   adjuvants for breast cancer treatment.","palabras_clave":"HISTAMINE|BREAST CANCER|H4 RECEPTOR LIGANDS|CELL PROLIFERATION|APOPTOSIS|CLOZAPINE|JNJ28610244"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64097,"idioma_id":4,"titulo":"Protection of Radiation-Induced Damage to the Hematopoietic System, Small Intestine and Salivary Glands in Rats by JNJ7777120 Compound, a Histamine H4 Ligand","resumen":"Based on previous dat on the histamine radioprotective effect on highly radiosensitive tissues, in the present work we aimed at investigating the radioprotective potential of the H4R ligand, JNJ7777120, on ionizing radiation-induced injury and genotoxic damage in small intestine, salivary glands and hematopouietic tissue.","palabras_clave":"JNJ7777120|IONIZING RADIATION|RADIOPROTECTOR|SMALL INTESTINE|BONE MARROW|SALIVARY GLANDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64098,"idioma_id":4,"titulo":"Biochemical approaches to C4 photosynthesis evolution studies: the case of malic enzymes decarboxylases","resumen":"C4 photosynthesis enables the capture ofatmospheric CO2 and its concentration at the site ofRuBisCO, thus counteracting the negative effects of lowatmospheric levels of CO2 and high atmospheric levels ofO2 (21 %) on photosynthesis. The evolution of this complexsyndrome was a multistep process. It did not occur bysimply recruiting pre-exiting components of the pathwayfrom C3 ancestors which were already optimized for C4function. Rather it involved modifications in the kineticsand regulatory properties of pre-existing isoforms of nonphotosyntheticenzymes in C3 plants. Thus, biochemicalstudies aimed at elucidating the functional adaptations ofthese enzymes are central to the development of an integrativeview of the C4 mechanism. In the present review, themost important biochemical approaches that we currentlyuse to understand the evolution of the C4 isoforms of malicenzyme are summarized. It is expected that this informationwill help in the rational design of the best decarboxylationprocesses to provide CO2 for RuBisCO in engineering C3species to perform C4 photosynthesis.","palabras_clave":"C4 ENZYMES|MALIC ENZYMES|KINETIC AND STRUCTURAL PROPERTIES|MOLECULAR AND BIOCHEMICAL TECHNOLOGIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64099,"idioma_id":4,"titulo":"A novel method for purification of polymerizable tubulin with a high content of the acetylated isotype","resumen":"Tubulin can be acetylated\/deacetylated on Lys40 of the &#945;-subunit. Studies of the post-translational acetylation\/deacetylation of tubulin using biochemical techniques require tubulin preparations that are enriched in AcTubulin (acetylated tubulin) and (for comparison) preparations lacking AcTubulin. Assembly-disassembly cycling of microtubules gives tubulin preparations that contain little or no AcTubulin. In the present study we demonstrated that this result is owing to the presence of high deacetylating activity in the extracts. This deacetylating activity in rat brain homogenates was inhibited by TSA (Trichostatin A) and tubacin, but not by nicotinamide, indicating that HDAC6 (histone deacetylase 6) is involved. TSA showed no effect on microtubule polymerization or depolymerization. We utilized these properties of TSA to prevent deacetylation during the assembly-disassembly procedure. The effective inhibitory concentration of TSA was 3 &#956;M in the homogenate and 1 &#956;M in the subsequent cycling steps. By comparison with immunopurified AcTubulin, we estimated that ~64% of the tubulin molecules in the three cycled preparations were acetylated. The protein profiles of these tubulin preparations, as assessed by SDS\/PAGE and Coomassie Blue staining, were identical to that of a preparation completely lacking AcTubulin obtained by assembly-disassembly cycles in the absence of TSA. The tyrosination state and in vitro assembly-disassembly kinetics were the same regardless of the degree of acetylation.","palabras_clave":"Microtubules|Acetylated tubulin|Tubulin Purification"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64100,"idioma_id":4,"titulo":"Estimation of Volume Rendering Efficiency with GPU in a Parallel Distributed Environment","resumen":"Visualization methods of medical imagery based on volumetric data constitute a fundamental tool for medical diagnosis, training and pre-surgical planning. Often, large volume sizes and\/or the complexity of the required computations present se- rious obstacles for reaching higher levels of realism and real-time performance. Performance and efficiency are two critical aspects in traditional algorithms based on complex lighting models. To overcome these problems, a volume rendering algo- rithm, PD-Render intra for individual networked nodes in a parallel distributed architecture with a single GPU per node is presented in this paper. The implemented algorithm is able to achieve photorealistic rendering as well as a high signal-to- noise ratio at interactive frame rates. Experiments show excellent results in terms of efficiency and performance for rendering medical volumes in real time","palabras_clave":"Volume Rendering; |Monte Carlo Method; Ray Tracing; |GPU; Scientific Visualization; |Cluster Computing; Parallel Processing; efficiency"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64101,"idioma_id":4,"titulo":"A New Record of James's Flamingo (Phoenicoparrus jamesi) from Laguna Melincué, a Lowland Wetland in East-Central Argentina","resumen":"James´s Flamingos (Phoenicoparrus jamesi), also known as Puna Flamingos, are distributed primarily in the Andean wetlands of southern South America during the breeding season, and dispersed through the lowland wetlands in Argentina during the non-breeding season. However, the main sites where they concentrate during the non-breeding season still remain unknown. Here, we report the first records of James?s Flamingos observed in two consecutive years (two adults in Aug 2010, one adult and two subadults in Jul 2011) at Laguna Melincue´, a Ramsar site in Santa Fe Province, Argentina (33u 259 S, 61u 289 W, 84 m asl). James?s Flamingos were feeding in a mixed flock of Andean and Chilean flamingos Phoenicoparrus andinus and Phoenicopterus chilensis, respectively). Our observations constitute the southernmost record for presence of this flamingo species at any lowland site and indicate the high dispersal ability of this species and its plasticity in occupying different wetland types during the non-breeding season.","palabras_clave":"argentina |James flamingo|Laguna Melincue|Ramsar site"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64102,"idioma_id":5,"titulo":"Una herramienta de Simulación para la Planificación de Procesos","resumen":"El principal objetivo de los Sistemas Operativos (SOs) enun ambiente de computación es la gestión de los recursosdel sistema. El recurso más importante de todo sistema esla CPU y por lo tanto administrarlo de forma eficienteimplica realizar una cuidadosa planificación de su uso. Espor ello que realizar una correcta planificación constituyeuno de los puntos centrales en el diseño de un buen SO.Comprender la importancia de la administración de losrecursos generalmente se basa en el análisis de conceptosteóricos con prácticas de escritorio. Ayudar a lametodología de enseñanza tradicional mediante laexperimentación permitirá comprender las característicasy funcionalidades del administrador del procesador,objetivo de este trabajo.SPPP es un Simulador del Planificador de Procesos; suobjetivo es realizar una correcta simulación de laplanificación de los procesos bajo distintas cargas delsistema, permitiéndole al usuario la definición delambiente de trabajo a recrear. La utilización de SPPPpermite comparar el desempeño de una política deplanificación con respecto a otra, pudiéndose establecercuál de ellas se comporta mejor bajo una determinadaconfiguración.En este trabajo se presentan las consideracionesprincipales tanto del diseño como de la implementación deSPPP, además de los resultados obtenidos en el dictado dematerias relacionadas a los SOs.","palabras_clave":"Sistema Operativo|Simulación|Planificador de Procesos| CPU y Software Libre."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64103,"idioma_id":4,"titulo":"Survey of toxigenic fungi and natural co-occurrence of mycotoxins in poultry feeds from Entre Ríos, Argentina","resumen":"A total of 120 pelleted poultry feed samples from Entre Ríos Province,  Argentina were evaluated. The aim was to investigate: i) the presence of  significant toxigenic fungi, as well as to determine the ability to produce aflatoxins (AFs) by Aspergillus section Flavi strains isolated, and ii) to evaluate the natural co-occurrence of AFs, fumonisin (FBs), gliotoxin,diacetoxyscirpenol (DAS), HT-2 and T-2 toxin by HPLC-MS\/MS. Total fungal counts are below of the established value (1 x 104 CFU g-1). Aspergillus flavus and A. parasiticus were the only aflatoxigenic species isolated. Cooccurrence of fumonisin B1 (FB1), HT-2 and T-2 toxin were detected in 100% of the feeds in mean levels ranged from 4501.7 to 5813.4; 6.7 to 21.6 and 19.6 to 30.3 ?g kg-1, respectively. A high number of starter samples were co-contaminated with aflatoxin B1 (AFB1), FB1, HT-2 and T-2 toxins. Gliotoxin and DAS were not found in this survey.","palabras_clave":"mycotoxins|fungi|Co-occurrence"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64104,"idioma_id":4,"titulo":"Dams in the last large free-flowing rivers of Patagonia, the Santa Cruz River, environmental features, and macroinvertebrate community","resumen":"Three large rivers have their headwaters in the Patagonian Ice Fields (PIFs) in the Andes Mountains, thelargest mid-latitude ice masses on Earth: Santa Cruz, Baker and Pascua. They are the last large free flowingrivers in Patagonia, but plans are advanced for building dams for hydroelectric power generation. Thethree PIF rivers, with a discharge dominated by ice melt, share a common, unique hydrograph comparedto that of the other eight large rivers in the region: a distinct seasonal cycle, and an extremely stable dis-charge, with much lower variability than other rivers. In this study we present the first extensive surveyof habitats and benthic macroinvertebrates in the least studied system, the Santa Cruz River. We assesshow much of the natural capital provided and sustained by benthic invertebrates are expected to be lostby flooding and discuss how dams would affect riverine habitat and biota. In the Santa Cruz River, weconducted an intensive field survey during September 2010; a total of 52 sites located at regular 6 kmintervals were sampled along the 310 river-km for macroinvertebrates and seventeen habitat variables.Although some habitat structure is apparent at the local scale, the Santa Cruz River could be describedas very homogeneous. Macroinvertebrate density and the richness (38 genera) found in the Santa CruzRiver resulted to be one of the lowest in comparison with 42 other Patagonian rivers. Albeit weak, thestructure of the macroinvertebrates assemblages was successfully described by a reduced set of variables.The reduced flow variation and the lack of bed scouring flows have a direct and negative effect on theheterogeneity of riverbeds and banks. The high turbidity of the Santa Cruz River may also contribute toshorter food webs, by affecting autotrophic production, general trophic structure, and overall macroin-vertebrate productivity and diversity. Dams will obliterate 51% of the lotic environment, including themost productive sections of the river according to our macroinvertebrate data. Since Santa Cruz River hasa naturally homogeneous flow cycle, dams may provide more variable flows and more diverse habitat.Our data provide critically valuable baseline information to understand the effects of dams on the uniqueset of glacial driven large rivers of Patagonia.","palabras_clave":"HYDROLOGY|GLACIER RIVERS|HABITAT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64105,"idioma_id":5,"titulo":"Análisis Multivariante, Conceptos y Aplicaciones en Psicología Educativa y Psicometría","resumen":"El objetivo de este trabajo radica, por una parte, en brindar un panorama introductorio sobre distintos conceptos de Análisis Multivariante (AM) y, por otra, proporcionar información acerca de diferentes aplicaciones realizadas en el área de la Psicología Educativa y de la Psicometría. La disponibilidad de programas informáticos, el progreso de la tecnología y el desarrollo de la teoría multivariante, han producido en las últimas décadas un notable crecimiento en la utilización de métodos y modelos estadísticos en todos los campos de la investigación científica. Las técnicas que conforman el AM permiten abordar distintos problemas de predicción, clasificación y segmentación, habitualmente presentes en las realidades de diversas áreas de conocimiento","palabras_clave":"ANALISIS MULTIVARIANTE|MODELIZACIÓN ESTADÍSTICA|PSICOLOGÍA EDUCATIVA|PSICOMETRÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64106,"idioma_id":4,"titulo":"Pharmacodynamic study of the 7,8-dihydroxy-4-methylcoumarin-induced selective cytotoxicity toward U-937 leukemic cells versus mature monocytes: Cytoplasmic p21(Cip1\/WAF1) as resistance factor.","resumen":"The development of tumor-selective drugs with low systemic toxicity has always been a major challenge in cancer treatment. Our group previously identified the 7,8-dihydroxy-4-methylcoumarin (DHMC) as a potential chemotherapeutic agent due to its potent, selective anti-proliferative and apoptosis-inducing effects on several cancer cell lines over peripheral blood mononuclear cells. However, there are still no published reports that can explain such selectivity of action. Herein, we addressed this question by using the U-937 promonocytic leukemia cell line, which can be forced to differentiate into a monocyte-like phenotype in vitro. U-937 cells differentiation is dependent on the nuclear expression of p21Cip1\/WAF1, a protein that is absent in immature U-937 cells but present in both the nucleus and the cytoplasm of normal DHMC-resistant monocytes. Considering that induction of differentiation rendered U-937 cells resistant to DHMC, we evaluated the possible causal role of cytoplasmic p21Cip1\/WAF1 in the onset of such resistance by employing U-937 cells stably transfected with a ZnCl2-inducible p21Cip1\/WAF1 variantlacking the nuclear localization signal (U-937\/CB6-DNLS-p21 cells). Expression of cytoplasmic p21Cip1\/ WAF1 did not induce differentiation of the cells but turned them resistant to DHMC through inhibition of JNK, a crucial mediator of DHMC-induced apoptosis in U-937 cells. Sub-acute toxicity evaluation of DHMC in Balb\/c mice indicated that DHMC administered intraperitoneally at doses up to 100 mg\/kg induced no systemic damage. Collectively, our results explain for the first time the selective cytotoxicity of DHMC for tumor cells over normal monocytes, and encourage further in vivo studies on this compound as potential anti-leukemic agent.","palabras_clave":"CANCER|COUMARICS|PROLIFERATION|MONOCYTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64107,"idioma_id":4,"titulo":"Hemolysin from Escherichia coli induces oxidative stress in blood","resumen":"Hemolysin (HlyA) produced by some stains of Escherichia coli is considered to be an important virulence factor of those bacteria. On the other hand, reactive oxygen species (ROS) have been reported to be involved in the pathogenesis of different diseases via oxidative stress generation. The purpose of this study was to analyze the capacity of HlyA to induce oxidative stress in whole blood cultures (WBCs). To this end, ROS production, the damage induced in lipids and proteins, and the antioxidant defense system was evaluated in blood cultures exposed to low concentrations of HlyA. We found that HlyA increased the level of free radicals detected by chemiluminescence assay. Moreover, lipid peroxidation and protein damage was significantly increased in cultures treated with HlyA in comparation with those found in control cultures. On the other hand, a decrease in total antioxidant capacity of plasma and in the activity of superoxide dismutase (SOD) was observed in plasma from blood treated with HlyA. Collectively, our data demonstrate that low concentrations of E. coli hemolysin induced oxidative stress in WBCs with the induction of different oxidative damage biomarkers.","palabras_clave":"Hemolysin|E coli"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64108,"idioma_id":4,"titulo":"Knock-down of a member of the Isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris","resumen":"Flavonoids and isoflavonoids participate in the signaling exchange between roots of legumes and nitrogen-fixing rhizobia and can promote division of cortical cells during nodule formation by inhibiting auxin transport. Here, we report the characterization of a member of the common bean isoflavone reductase (EC 1.3.1.45, PvIFR1) gene family, an enzyme that participates in the last steps of the biosynthetic pathway of isoflavonoids. Transcript levels of PvIFR1 were detected preferentially in the susceptible zone of roots, augmented upon nitrogen starvation and in response to Rhizobium etli inoculation at very early stages of the interaction. Knockdown of PvIFR1 mediated by RNA interference (RNAi) in common bean composite plants resulted in a reduction of shoot and root length. Furthermore, reduction of PvIFR1 mRNAs also affected growth of lateral roots after emergence, a stage in which auxins are required to establish a persistent meristem. Upon inoculation, the number of nodules formed by different strains of R. etli was significantly lower in IFR RNAi than in control roots. Transcript levels of two auxin-regulated genes are consistent with lower levels of auxin in PvIFR1 silenced roots. These results suggest a complex role of PvIFR1 during plant growth, root development and symbiosis, all processes in which auxin transport is involved.","palabras_clave":"Isoflavonoids|legume|Rhizobium|Nitrogen fixation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64109,"idioma_id":5,"titulo":"Técnicas experimentales para el estudio de la Angiogénesis en enfermedades Reproductivas Femeninas","resumen":"El desarrollo vascular en el tracto reproductivo femenino es un proceso que se encuentra restringido a la formación del cuerpo lúteo y a la proliferación del endometrio uterino durante los ciclos menstruales. Han sido identificados numerosos inductores de la angiogénesis. Estos incluyen, entre otros, a los miembros del factor de crecimiento fibroblástico (FGF) y a la familia del factor de crecimiento del endotelio vascular (VEGFA), angiopoyetinas (ANGPTs), factor de crecimiento transformante (TGF) y al factor derivado de plaquetas (PDGF). Los defectos en la angiogénesis ovárica contribuyen a una variedad de desórdenes, incluyendo anovulación e infertilidad, pérdida de embarazo, Síndrome de Hiperestimulación Ovárica (OHSS), Síndrome de Poliquistosis Ovárica (PCOS) y neoplasmas ováricos. Es decir, el endotelio es una fuente y un blanco de sustancias vasoactivas que son secretadas debido a las condiciones que se desencadenan durante estos desordenes mencionados. Para evaluar los mecanismos bioquímicos y moleculares que participan en la angiogénesis se han desarrollado métodos in vitro (migración por herida, tubulogénesis, etc) e in vivo (implante de Matrigel, membrana corionalantoide, etc). Los ensayos in vitro pueden ser útiles para validar los primeros pasos de la angiogénesis pero deben ser confirmados por estudios in vivo. En particular, los ensayos in vitro e in vivo que fueron descriptos en este trabajo para evaluar el desarrollo de los vasos, pueden ser aplicados para el estudio de desórdenes reproductivos con alteraciones en la angiogénesis (OHSS, PCOS, cáncer de ovario, etc.) mediante la utilización del suero, líquido peritoneal, fluido folicular o de biopsias de distintos tejidos provenientes de las pacientes.","palabras_clave":"ANGIOGENESIS|REPRODUCCION FEMENINA|ENDOTELIO|PERICITOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64110,"idioma_id":5,"titulo":"'Limitaciones al poder municipal ante la libertad de publicidad interior'","resumen":"El presente artículo se enraíza en un fallo del Superior Tribunal de Mendoza por medio del cual se resolvió anular diversas determinaciones de oficio de derechos de publicidad y propaganda realizadas por la Municipalidad de Guaymallén contra una empresa embotelladora. Tales determinaciones habían hallado su origen en la presunta exhibición de 'publicidad interior' de dicha empresa embotelladora en diversos comercios ubicados en el ejido municipal. Con ocasión del referido precedente, en esta oportunidad se ha examinado: i) cuál es la naturaleza jurídica de los derechos de publicidad y propaganda (si se trata de cánones, tasas o impuestos); ii) si tales derechos son compatibles con el Régimen de Coparticipación Federal; iii) si es legítima su aplicación retroactiva.","palabras_clave":"LEGALIDAD|TRIBUTO|COPARTICIPACIÓN|MUNICIPIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64111,"idioma_id":5,"titulo":"Otra vuelta de tuerca sobre Dennett y la hermenéutica artefactual: tensiones y aporías","resumen":"Este trabajo versa sobre la aplicación a los artefactos técnicos del enfoque filosófico propuesto por Daniel Dennett para elucidar el ámbito de las cosas artificiales. En particular, sugiere dos cosas. Por una parte, que esta aplicación no nos permite entender acabadamente la dimensión normativa que cubre la esfera práctica de nuestra producción y uso de artefactos técnicos. Por otra, que ella promueve un criterio sumamente liberal de la atribución de funciones a los artefactos técnicos que desfigura la idea misma de función técnica. Este trabajo tiene tres partes. En la primera se caracteriza la hermeneútica artefactual promovida por la propuesta dennettiana. Se analiza, especialmente, las tres actitudes dennettianas, a saber, la actitud física, la actitud del diseño y la actitud intencional. Además, se considera el punto de vista de la ingeniería inversa. En la segunda parte se discute la analogía entre textos y artefactos y se reconstruye la estrategia incrustada en esta analogía. En la última parte se presenta un balance crítico de la propuesta filosófica de Dennett en su aplicación al ámbito de los artefactos técnicos.","palabras_clave":"hermenéutica|artefacto técnico|Dennett|normatividad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64112,"idioma_id":4,"titulo":"Performance of different Trypanosoma cruzi antigens in the diagnosis of Chagas disease in patients with American Cutaneous Leishmaniasis from a co-endemic region in Argentina","resumen":"objective To determine the ability of recombinant antigens to detect cases of infection with  Trypanosoma cruzi among cases of infection with Leishmania spp. by serological methods.  methods Sera from 41 patients infected with Leishmania spp. were evaluated with ELISA using  single (FRA, CP1 and TSSAVI) or pooled (commercial Rec-ELISA) recombinant proteins or  homogenate antigens (commercial H-ELISA). As there is no gold standard antigen to discriminate  Chagas disease from leishmaniasis, the correlation of results between de&#64257;ned antigens and the  homogenate was made with Kappa Index (KI), the level of correlation considered being used as a  criterion of speci&#64257;city.  results Single recombinant antigens and Rec-ELISA showed good correlation (KI > 0.8). A low  correlation (KI < 0.66) was observed between the results from single recombinant antigens or the  commercial recombinant kit and H-ELISA.  conclusions The highly correlated results between T. cruzi single or pooled recombinant proteins  are indicative of the usefulness of recombinant antigens for Chagas diagnosis. Our results also  indicate that in the city of Oran in Argentina, between 12% and 17% of patients with leishmaniasis  are also infected with Chagas disease. The high KI values between TSSAVI and the other  recombinant proteins suggest that in these patients, the infection may be caused by T. cruzi II and\/or  V and\/or VI lineages.","palabras_clave":"recombinant agent|Trypanosoma cruzi|leishmaniasis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64113,"idioma_id":4,"titulo":"Computational tools for the simulation  of atmospheric pollution events.","resumen":"The present work briefly details the theoretical aspects of numerical methods for the simulation of turbulent transport and dispersion of gases into the atmospheric boundary layer. Later, we show some laboratory experiments of plume dispersion emitted from contaminant sources and the results of the computational simulation tool in comparison with laboratory simulations. Finally, we present a simulation of a hypothetical delocalisation of an open dump close to the city of Paraná in the province of Entre Ríos, Argentina. The results shown include gas concentrations, as well as their geographical distribution from a downwind source, under the most unfavorable meteorological situation.","palabras_clave":"Atmospheric pollution|Large-eddy Simulation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64114,"idioma_id":4,"titulo":"Unsaturated polyester\/bentonite nanocomposites: influence of clay modification on final performance","resumen":"In this work, unsaturated polyester\/bentonite nanocomposites were obtained and characterized. The bentonite used was unmodi&#64257;ed and with different chemical treatments. The effects of these different  chemical modi&#64257;cations (cation exchange reactions with quaternary ammonium and phosphonium salts) of this clay as well as the effect of clay content on the thermal, barrier (water absorption), mechanical  (&#64258;exural) and dynamic-mechanical properties of unsaturated polyester matrix were analyzed. The results clearly show that the chemical modi&#64257;cations of the clay cause a desired effect on its &#64257;nal properties  improving the performance of the nanocomposites. The enhancements could be directly related to the dispersion of the clay inside the matrix, as shown by transmission electron microscopy.","palabras_clave":"Nanostructures|Polymer-matrix composites|Mechanical properties|Thermosetting resins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64115,"idioma_id":4,"titulo":"Continental phylogeography of an ecologically and morphologically diverse neotropical songbird, Zonotrichia capensis","resumen":"Background: The Neotropics are exceptionally diverse, containing roughly  one third of all extant bird species on Earth. This remarkable species richness  is thought to be a consequence of processes associated with both Andean orogenesis  throughout the Tertiary, and climatic fluctuations during the Quaternary.  Phylogeographic studies allow insights into how such events might have  influenced evolutionary trajectories of species and ultimately contribute to a  better understanding of speciation. Studies on continentally distributed  species are of particular interest because different populations of such taxa  may show genetic signatures of events that impacted the continentwide biota.  Here we evaluate the genealogical history of one of the world´s most  broadly-distributed and polytypic passerines, the rufous-collared sparrow (Zonotrichia  capensis). Results: We obtained control region DNA sequences from 92 Zonotrichia  capensis individuals sampled across the species´ range (Central and South  America). Six additional molecular markers, both nuclear and mitochondrial,  were sequenced for a subset of individuals with divergent control region  haplotypes. Median-joining network analysis, and Bayesian and maximum parsimony  phylogenetic analyses all recovered three lineages: one spanning Middle America,  the Dominican Republic, and north-western South America; one encompassing the  Dominican Republic, Roraima (Venezuela) and La Paz (Bolivia) south to Tierra  del Fuego, Argentina; and a third, including eastern Argentina and Brazil. Phylogenetic  analyses suggest that the Middle American\/north-western South American clade is  sister to the remaining two. Bayesian and maximum likelihood coalescent  simulations used to study lineage demographic history, diversification times,  migration rates and population expansion together suggested that diversification  of the three lineages occurred rapidly during the Pleistocene, with negligible  gene flow, leaving genetic signatures of population expansions. Conclusions: The  Pleistocene history of the rufous-collared sparrow involved extensive range  expansion from a probable Central American origin. Its remarkable morphological  and behavioral diversity probably represents recent responses to local  conditions overlying deeper patterns of lineage diversity, which are themselves  produced by isolation and the history of colonization of South America.","palabras_clave":"COLONIZATION|DEMOGRAPHIC EXPANSION|INTRASPECIFIC DIVERGENCE|DNA SEQUENCES|PLEISTOCENE|RUFOUS-COLLARED SPARROW"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64116,"idioma_id":4,"titulo":"Assesing The Performance of Different S-Metaheuristics To Solve Unrestricted Parallel Identical Machines Scheduling Problem","resumen":"In this paper we present a comparative study of four trajectory or single-solution based metaheuristics (S-metaheuristics): Iterated Local Search (ILS), Greedy Randomized Adaptive Search Procedure (GRASP), Variable Neighborhood Search (VNS), and Simulated Annealing (SA). These metaheuristics were consideredto assess their respective performance to minimize the Maximum Tardiness (Tmax) for the unrestricted parallel identical machine scheduling (Pm) problem, which is considered an NP-Hard problem. The results obtained through experimentation show that SA was the best performing metaheuristic.","palabras_clave":"Iterated Local Search|Greedy Randomized Adaptive Search Procedure|Variable Neighborhood Search|ParallelMachines Scheduling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64117,"idioma_id":4,"titulo":"New footprints into metazoan C-1 family cadherin evolution","resumen":"Specific cell-cell adhesion and intracellularcommunication are key processes in multicellular animals where in cadherin superfamilymembers are essential players. Evolutionary origin of the modern C-1 cadherin family inmetazoans was determined using a Neighbor-Joining (NJ) algorithm and the EC1 first cadherinrepeatsequence ??avery short segment of the full length proteins responsible for adhesiverecognition ?? and their cytoplasmic domain. Searching for insights that reveal organizationalchanges in C-1cadherins, a phylogenetic analysis of full extracellular and cytoplasmic domainsfrom 8 vertebrate species was performed, using Maximum Likelihood phylogenetic algorithm.The amino acid sequence homology between extracellular and cytoplasmic domains wereanalyzed and the protein structural data architecture was reviewed. The analysis of fullextracellular length of C-1 cadherins suggests that the CDH13-solitary cadherin must bereclassified into classical type I cadherins. Specifically, it was demonstrated that EC2 to EC5repeats addition to EC1 have significant evolutive information, changing the previouslyproposed molecular phylogeny. In contrast, the CDH26 solitary cadherin, in agreement with theHulpiau and van Roy (10) proposal, must be placed between desmogleins and desmocollins.Taking together CDs-alignments and the different evolutive models proposed for the EC- andCD-based trees, seem to be clear that EC and CD have evolved separately. Therefore, thedifferences between CD subfamilies could have developed the competence of cadherins tointeract with different partner cytosolic proteins to participate in different signal pathwaycontrolling the cell life.","palabras_clave":"Cadherin C-1|Evolution|Metazoan"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64118,"idioma_id":4,"titulo":"Phytophthora multivora causing leaf spot on rhododendrons in Argentina.","resumen":"Rhododendron is a genus belonging to the family Ericaceae and contains over 1000 species commonly known as rhododendrons and azaleas (Dimitri, 1978). Phytophthora multivora is a species that was described in Western Australia in 2008 but before that it was misidentified as . citricola (Scott et al., 2009). During the early spring of 2011, leaf spot symptoms were observed on rhododendrons in two gardens in Tigre (northern Buenos Aires province) and in containers in a nursery near Buenos Aires city. Leaf spots were dark brown to almost black, visible on both sides near the leaf tips and margins, while tissue death continued down the leaf along the midrib (Fig. 1A). The veins under the diseased area of the leaves presented a reddish tint (Fig. 1B). Some leaves became brown and died, while in others the infection remained as spots on leaves.    A Phytophthora species was consistently isolated from symptom-bearing leaf tissues on PARBH medium (Jeffers & Martin, 1986). Hyphal tips reaching the surface of the medium were transferred onto potato dextrose agar (PDA) without inhibitors for purification and identification. Sporangia were produced abundantly in non-sterile soil extract. The majority of them were semi-papillate and ovoid, limoniform, ellipsoid or obpyriform (Fig. 2). Sporangia with two papillae were occasionally formed (Scott et al., 2009). Chlamydospores were not observed. Isolates were homothallic with plerotic oospores, 22.9 ± 1.9 &#956;m and paragynous antheridia (Fig. 3). The optimum growth temperature was 25 ± 1°C on V8A (Scott et al., 2009) and the maximum growth temperature was 32 ± 1°C. The ITS was amplified and sequenced (GenBank Accession No. JQ812127) and showed that it was identical to P. multivora, ex-type CBS 124.094 (FJ237517) by BLAST analysis (Altschul et al., 1997). The isolate (ARod 110) was deposited in the culture collection of the Phytopathology Chair of the Faculty of Agronomy of Buenos Aires (FAUBA).    Due to the difficulty of performing pathogenicity tests in the field, detached leaves of Rhododendron spp., Camellia sp. Viburnum tinus and Photinia fraseri were inoculated in the laboratory with the ARod 110 isolate. The tests were performed by inoculating five detached leaves with a 5 mm mycelium plug taken from a seven-day-old PDA culture. Controls were inoculated with PDA discs. Leaves were incubated at 20-22ºC under 12h light\/12h dark cycle. All the inoculated leaves, except Camellia sp., developed necrotic lesions seven days after inoculation. . multivora was re-isolated from infected tissue. Symptoms were not detected on the controls. These characteristics conformed to those of Phytophthora multivora Scott & Jung. To our knowledge, this is the first record of P. multivora causing leaf spot on Rhododendron in Argentina and in Latin America, and it may be a potential pathogen for Viburnum tinus and Photinia fraseri.","palabras_clave":"Phytophthora multivora|Mancha Foliar|Azalea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64119,"idioma_id":4,"titulo":"Interespecific Hybridization among Three Species of Glandularia (Verbenaceae) Native to Argentina","resumen":"Glandularia J.F. Gmelin (Verbenaceae) is a genus with high ornamental  and economic value. Breeding system of G.  peruviana, G. platensis and G. glandulifera was studied, and the  results indicated the existence of self-incompatibility. This facilitates interspecific hybridization,  which is one of the most important sources of genetic variation in breeding  ornamentals. The Catholic University of Córdoba has initiated a Glandularia breeding program with the  aim to obtain compact plants adapted to growing conditions of our country. Compactness  is not a common characteristic in native germplasm. With the objective of determine thereproductive efficiency &nbsp;of species and interspecific compatibility  in the genus Glandularia, diallel  crosses were carried out among three species. Reproductive efficiency was  calculated. The pollen tube growth helped identify barriers to interspecific  hybridization. The highest values of reproductive efficiency were for G. glandulifera × G. peruviana and G. peruviana × G. glandulifera. The  lowest values were found when G.  platensis was used as female parent. The cross of G. platensis × G. peruviana did not produce seeds. In those crosses  in which pollen tube was able to growth through the stigma, barriers to  hybridization were found along the style, at the ovary apex and\/or penetrating  the micropyla. G. peruviana and G. glandulifera behave better as female  parent, and the crosses between G. glandulifera  × G. peruviana yielded the largest number of interspecific hybrids and the  number of plants that survive to the adult stage. These results show the  compatibility, the maternal ability to produce seeds, and the place where there  are barriers to hybridization to overcome if necessary.                  &nbsp;","palabras_clave":"breeding|incompatibility|pollen tube growth|ornamentals"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64120,"idioma_id":4,"titulo":"Calibrachoa Pollen Viability over Time at Varying Storage Temperatures","resumen":"Pollen storage strategies are desirable in several crop species for different purposes. Calibrachoa La Llave &amp; Lexarza (Solanaceae) is a South American genus (Stehmann and Semir, 2005) having a high ornamental value resulting in the introduction of many successful commercial varieties for pot and landscaping use. The Institute of Floriculture INTA Castelar (IF) has initiated a Calibrachoa breeding program to obtain compact varieties, with no pruning or plant growth regulator requirements. The ability to store Calibrachoa pollen and maintain viability over extended periods of time offers flexibility to plant breeders to use stored pollen to use in hybridization without parents flowering synchronously. There are no records about the long-term storage of Calibrachoa pollen. The objective of this study was to know the long-term pollen storage of C. humilis, C. caesia and C. ovalifolia pollen which are the most important species in the breeding program of the IF. For this purpose, pollen grains were dried and placed at different storage duration for two (2M), six (6M) and eight (8M) months. A hanging drop pollen germination assay was used to determine the percentage of pollen germination (% PG). The studies allowed detecting a marked decrease in pollen viability after 2M storage in all the treatments for all species. During this time, the decrease in germination percentage in C. caesia, C. humilis, and C. ovalifolia ranged between 23.9 and 38.84%. The percentages of viability at 8M storage were below 10%. Although after two months of storage there were pollen viability percentages of 50% in the three analyzed species, none of the treatments tested showed better performance than the others. The results suggest that the Calibrachoa species have a medium-lived pollen longevity (approximately 1-3 months).","palabras_clave":"mdium-lived pollen longevity|pollen conservation|solanaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64121,"idioma_id":4,"titulo":"Compatibility Study in Intergeneric Crosses between Nierembergia and Calibrachoa","resumen":"In thepresent study, we report three methods to overcome pre-fertilization barriersin intergeneric crosses between Nierembergia and Calibrachoa, as a tool for ornamental plantbreeding programs. Althoughpre-fertilization studiesrevealed the incompatibilityamong Nierembergia and Calibrachoa species, expressed in the pistil, four intergeneric combinations (N. scoparia x C. ovalifolia; N. scopariax C. caesia; N. linariaefolia x C.thymifolia and N. linariaefolia xC. ovalifolia) were detected asputative crosses to obtainhybrids. Pollination was performed by three methods: 1) bud pollination, 2)cut-style pollination and 3) heating the style before pollination (50º C, for 2.5-5min). When N. scopariawas used as the mother plant, the percentage of pollen tubes in the ovaryincreased significantly in the bud-pollination method and to a lesser extentwhen heating the style before pollination. In contrast,when N. linariaefolia was used as themother plant, no pollen tubes were found in the ovary. On the other hand, the cut-stylepollination method was not successful in the combinations evaluated because theabsence of stigma affected pollen germination negatively. The results suggest thatbud-pollination could be the best method to overcome pre-fertilization barriersin N. scoparia x C. ovalifolia and N. scoparia x C. caesia crosses. The results of the present study will serve as abasis for the future research of post-fertilizationbarriers, as a tool to obtain hybridsbetween both genera.","palabras_clave":"Fertilization|Hybridization |Pollen tube growth|Solanaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64122,"idioma_id":4,"titulo":"Chromosome studies and the Implications on Ornamental Characteristics in Passiflora mooreana (Passifloraceae)","resumen":"The genus Passiflora (Passifloraceae) comprises more than 500 species and it is considered an important genetic resource. Among Argentinian native species, P. mooreana have phenotypic characteristics to make it a candidate to participate in the breeding program carried out in the Instituto de Floricultura, (Institute of Floriculture, National Institute of Agronomic Technology, Argentina). The purpose of this program is to develop, by interspecific crosses, new attractive and cold tolerant varieties. The aim of this work was to characterize morphologically and citologically two genotypes of P. mooreana collected in the provinces of Corrientes and Formosa (Argentina). It does not exist previous register of the chromosome number of P. mooreana, so this is especially important. Comparative measures of the width and length of the leaves and the flower diameter show statistical differences between the two genotypes and were higher in the one from Corrientes. No difference were found between the genotypes in the crown diameter and width of the petals. In flow-cytometric analysis, the Corrientes genotype shows twice the value of the Formosa genotype. The chromosomal number was 2n = 2x = 18 (Formosa) and 2n = 4x = 36 (Corrientes). The chromosome number provides to the Formosa genotype with higher probabilities of a successful cross with species from the same subgenus because they have the same chromosome number. The tetraployd genotype would have best chances to be crossed with tetraployd species or artificially poliploydized hybrids. Both genotypes show a percentage of pollen viability elevated enough to be considered as a male parent in crossings.","palabras_clave":"Chromosome number|poliploidy|Argentina native species|breeding ornamental|interspecific hybridization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64123,"idioma_id":4,"titulo":"Reproductive Biology of Habranthus tubispathus","resumen":"Habranthus tubispathus is a bulbous species native to the sub humid and semiarid Pampean regions of Argentina, with a high aesthetic ornamental value for sustainable landscaping. In order to initiate breeding programs, the reproductive biology of the species was investigated. Self- and cross-pollinations were done on plants collected from a natural population. Fruits set were recorded in both self- and cross-pollinated plants, as well as in open-pollinated plants from the natural population. The fructification ratio, the reproductive success (RS) and the compatibility index were obtained. Also, observations of pollen tube growth in pistils of both types of pollination were evaluated. Self- and cross-pollinated plants set fruits. The fruit setting was lower in self-pollinated plants (59.4%) as compared to cross-pollinated ones (91%), which showed similar setting as open-pollinated plants (93%). Accordingly, RS values were 0.26 and 0.37 for self-pollinated and cross-pollinated plants, respectively. The growth of the pollen tubes (more than 100 tubes reaching the ovary) and the quantity of seeds per fruit (53) did not differ among self and cross-pollinated plants. Another evidence of H. tubispathus outbreeding is the presence of herkogamy in flowers, despite that this species exhibits partially self-compatible with the compatibility index value of 0.65.","palabras_clave":"HERKOGAMY|POLLINATION|SEED SET|RAINLILY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64124,"idioma_id":4,"titulo":"Lack of the Matricellular Protein SPARC (Secreted Protein, Acidic and Rich in Cysteine) Attenuates Liver Fibrogenesis in Mice","resumen":"Introduction: Secreted Protein, Acidic and Rich in Cysteine (SPARC) is a matricellular protein involved in many biological processes and found over-expressed in cirrhotic livers. By mean of a genetic approach we herein provide evidence from different in vivo liver disease models suggesting a profibrogenic role for SPARC.  Methods: Two in vivo models of liver fibrosis, based on TAA administration and bile duct ligation, were developed on SPARC wild-type (SPARC+\/+) and knock-out (SPARC2\/2) mice. Hepatic SPARC expression was analyzed by qPCR. Fibrosis was assessed by Sirius Red staining, and the maturation state of collagen fibers was analyzed using polarized light. Necroinflammatory activity was evaluated by applying the Knodell score and liver inflammatory infiltration was characterized by immunohistochemistry. Hepatic stellate cell activation was assessed by a-SMA immunohistochemistry. In addition, pro-fibrogenic genes and inflammatory cytokines were measured by qPCR and\/or ELISA. Liver gene expression profile was analyzed in SPARC2\/2 and SPARC+\/+ mice using Affymetrix Mouse Gene ST 1.0 array.  Results: SPARC expression was found induced in fibrotic livers of mouse and human. SPARC2\/2 mice showed a reduction in the degree of inflammation, mainly CD4+ cells, and fibrosis. Consistently, collagen deposits and mRNA expression levels were decreased in SPARC2\/2 mice when compared to SPARC+\/+ mice; in addition, MMP-2 expression was increased in SPARC2\/2 mice. A reduction in the number of activated myofibroblasts was observed. Moreover, TGF-b1 expression levels were down-regulated in the liver as well as in the serum of TAA-treated knock-out animals. Ingenuity Pathway Analysis (IPA)analysis suggested several gene networks which might involve protective mechanisms of SPARC deficiency against liver fibrogenesis and a better established machinery to repair DNA and detoxify from external chemical stimuli.  Conclusions: Overall our data suggest that SPARC plays a significant role in liver fibrogenesis. Interventions to inhibit SPARC expression are suggested as promising approaches for liver fibrosis treatment.","palabras_clave":"SPARC|LIVER |FIBROGENESIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64125,"idioma_id":4,"titulo":"Lipoteichoic acid from Lactobacillus rhamnosus GG as an oral photoprotective agent against ultraviolet-induced carcinogenesis","resumen":"Probiotics are defined as 'live microorganisms that when administered in   adequate amounts confer a health benefit on the host'. Cell surface   molecules and extracellular components of these microorganisms are being   studied in relation to their ability to interact with the host. The   cell wall of lactobacilli comprises mainly peptidoglican and   lipoteichoic acids (LTA) which are macroamphiphilic molecules with   immunomodulatory properties, consisting of a glycerol-phosphate or   ribitolphosphate chain. Ultraviolet radiation (UVR) has been proposed as   the main cause of skin cancer because of its mutagenic and   immunosuppressive effects. Recently, the prevention of the harmful   effects of UVR has been demonstrated for some nutrients. Within these,   probiotics have emerged in the last few years as interesting candidates.   The aim of our study was to investigate whether the oral administration   of purified LTA from Lactobacillus rhamnosus GG can modulate the immune   suppressive effect of UVR and skin tumor development in mice. We found   that T cells in the inguinal lymph node of LTA treated animals produced   significantly higher levels of IFN-a, and that the number of total,   helper and cytotoxic T cells was increased. A significant delay in tumor   appearance was found in mouse treated with LTA, as well. The finding of   increased IgA+ cell number in small intestine and higher number of   activated dendritic cells in mesenteric lymph nodes may indicate a   direct effect of LTA in the gut, which could be affecting cutaneous   immune system and restoring homeostasis through the gut-skin axis.","palabras_clave":"LTA|LACTOBACILLUS  RHAMNOSUS GG |UVB"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64126,"idioma_id":4,"titulo":"Antibody labeling with Remazol Brilliant Violet 5R, a vinylsulphonic reactive dye","resumen":"Colloidal gold is the first choice for labeling antibodies to be used in Point Of Care Testing. However, there are some recent reports on a family of textile dyes ? named ´reactive dyes´? being suitable for protein labeling. In the present work, protein labeling conditions were optimized for Remazol Brilliant Violet 5R, and the sensitivity of the labeled antibodies was assessed and compared with that of colloidal-gold  labeled antibodies. Also, the accelerated stability was explored. Optimal conditions were pH 10.95, dye:Ab molar ratio of 264 and an incubation time of 132 minutes.  Labeled antibodies were stable, and could be successfully used in a slot blot assay, detecting as low as 400 ng\/ml. Therefore, the present work demonstrates that vinylsulphonic reactive dyes can be successfully used to label antibodies, and are excellent candidates for the construction of a new generation of Point of Care Testing kits.","palabras_clave":"ANTIBODY LABELING|REACTIVE DYES|REMAZOL|COLLOIDAL GOLD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64127,"idioma_id":5,"titulo":"Sequía e inundación en la hiperllanura Pampena: Una mirada desde el lote al municipio","resumen":"La variabilidad de las lluvias genera sequías e inundaciones que perjudican a campos y municipios. A nivel municipal frente al riesgo de inundación se reconoce el efecto crítico de las obras hidráulicas para defender ciudades y pueblos pero también su alcance limitado para evitar daños en la producción. Aún cuando persisten incertidumbres, a escala de un campo, el agua almacenada en el suelo y el nivel de la napa pueden ser usados para ajustar los sistemas de producción en el tiempo y en el espacio, permitiendo reducir los riesgos de sequías e inundaciones y aprovechar mejor las oportunidades. El gran desafío es idear formas de que las medidas de cambios en el uso de la tierra se propaguen a todo el territorio permitiendo moderar las consecuencias de años muy lluviosos, pero también muy secos, sobre un mayor número de productores y así también sobre el municipio.    Frente a la necesidad de innovar para resolver problemas en sistemas sociales complejos, conviene focalizar los esfuerzos en facilitar el trabajo en red y el aprendizaje conjunto, más que en trasferir mensajes o tecnologías (Engel 1995). La propagación diferencial desde el campo al municipio de la sequía y la inundación, por ejemplo, era difícil de prever desde una mirada externa al sistema. Tampoco resultaba claro con anterioridad al taller, que la sociabilización de un recurso asimétricamente distribuido en la comunidad, como el conocimiento universitario, pueda ser parte de la solución. La intensificación de espacios que permitan la interacción de los usuarios del territorio es una estrategia que puede facilitar su utilización más sensata.","palabras_clave":"AGRICULTURA|GANADERIA|INUNDACION|NAPA FREATICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64128,"idioma_id":4,"titulo":"Accelerating the parameters identifiability procedure:  Set by set selection","resumen":"In this paper, a numerical procedure based on the binary search is proposed for accelerating the parameters identifiability procedure. Basically, the parameters are selected set by set using a given criterion for ranking the parameters. Since parameters identifiability procedures are strongly dependent on the initial estimates of parameters values, simultaneous parameters re-estimation step has been proposed in this paper. Two examples were used to evaluate the performance of the proposed criterion. In both cases, a significant reduction of the computational time was observed, and the results regard to the model fit are similar to those criteria based on the selection of parameters one by one, as usually presented in the literature.","palabras_clave":"PARAMETERS IDENTIFIABILITY|BINARY SEARCH|PARAMETER ESTIMATION|SCARCE EXPERIMENTA DATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64129,"idioma_id":4,"titulo":"Latin american experience with Alzheimer's disease cerebrospinal fluid biomarkers","resumen":"A promising advance to complement clinicaldiagnosis at an early stage of Alzheimer\u2019s disease (AD)has been the measurement of biomarkers in cerebrospinalfluid (CSF).1 A decrease in CSF amyloid beta 42 (Ab42) isa marker of AD pathology caused by the accumulation ofamyloid-b in brain parenchyma,2 whereas increased totaltau (t-tau) and hyperphosphorylated tau (p-tau) reflectneuronal degeneration and tangle pathology.3 AD is characterizedby episodic memory loss, cognitive impairment,behavioral disorders, and finally, dementia.4 Individualswith mild cognitive impairment (MCI) have memory deficitsbut, in contrast to those with AD, are functionallyintact and at higher risk of converting to AD than thosewithout MCI.4 It is difficult to predict clinically whichindividuals with MCI possess AD brain pathology and willtherefore progress to clinical AD.The aim of the present study was to evaluate CSF ADbiomarkers in their capacity to discriminate AD fromfrontotemporal dementia (FTD) and to predict progressionfrom MCI to AD. Data are presented on the first experienceto the knowledge of the authors in Latin America ofthe use of CSF biomarkers in a clinical follow\u2013up study.","palabras_clave":"Dementia|Argentina|Alzheimer's|mild cognitive impairment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64130,"idioma_id":4,"titulo":"Trypanosoma cruzi carrying a monoallelic deletion of the calreticulin (TcCRT) gene are susceptible to complement mediated killing and defective in their metacyclogenesis","resumen":"Trypanosoma cruzi Calreticulin (TcCRT) can hijack C1, mannan-binding lectin and ficolins from serum thus inhibiting the classical and lectin complement pathway activation respectively. To understand the in vivo biological functions of TcCRT in T. cruzi we generated a clonal cell line lacking one TcCRT allele (TcCRT+\/-) and another clone overexpressing it (TcCRT+). Both clones were derived from the TCC T. cruzi strain. As expected, TcCRT+\/- epimastigotes showed impairment on TcCRT synthesis, whereas TcCRT+ ones showed increased protein levels. In correlation to this, monoallelic mutant parasites were significantly susceptible to killing by the complement machinery. On the contrary, TcCRT+ parasites showed higher levels of resistance to killing mediate by the classical and lectin but not the alternative pathway. The involvement of surface TcCRT in depleting C1 was demonstrated through restoration of serum killing activity by addition of exogenous C1. In axenic cultures, a reduced propagation rate of TcCRT+\/- parasites was observed. Moreover, TcCRT+\/- parasites presented a reduced rate of differentiation in in vitro assays. As shown by down- or upregulation of TcCRT expression this gene seems to play a major role in providing T. cruzi with the ability to resist complement system.","palabras_clave":"CALRETICULIN|COMPLEMENT|C1|KNOCK-OUT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64131,"idioma_id":4,"titulo":"Divergence times and the evolutionary radiation of New World monkeys (Platyrrhini, Primates): An analysis of fossil and molecular data","resumen":"              The estimation of phylogenetic relationships and divergence  times among a group of organisms is a fundamental first step toward  understanding its biological diversification. The&nbsp; time of the most recent or last common  ancestor (LCA) of extant platyrrhines is one of the most controversial among  scholars of primate evolution. Here we use two molecular based approaches to  date the initial divergence of the platyrrhine clade, Bayesian estimations under a  relaxed-clock model and substitution rate plus generation time and body size,  employing the fossil record and  genome datasets. We also explore the robustness of our estimations with respect  to changes in topology, fossil constraints and substitution rate, and discuss the implications of our findings  for understanding the platyrrhine radiation. Our results suggest that fossil  constraints, topology and substitution rate have an important influence on our divergence time  estimates. Bayesian  estimates using conservative but  realistic fossil constraints suggest that the LCA of extant platyrrhines existed  at ca. 29 Ma, with the 95% confidence limit for the node ranging from  27?31 Ma. The LCA of extant platyrrhine monkeys based on substitution rate  corrected by generation time and body size was established between 21?29 Ma. The estimates based on  the two approaches used in this study recalibrate the ages of the major  platyrrhine clades and corroborate the hypothesis that they constitute very old  lineages. These results can help reconcile several controversial points  concerning the affinities of key early Miocene fossils that have arisen among  paleontologists and molecular systematists. However, they cannot resolve the  controversy of whether these fossil species truly belong to the extant lineages  or to a stem platyrrhine clade. That  question can only be resolved by morphology. Finally, we show that the use of  different approaches and well supported fossil information gives a more robust  divergence time estimate of a clade.  ","palabras_clave":"DIVERGENCE|RADIATION|PLATYRRHINI|PHYLOGENETIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64132,"idioma_id":4,"titulo":"TcTASV-C, a Protein Family in Trypanosoma cruzi that Is Predominantly Trypomastigote-Stage Specific and Secreted to the Medium","resumen":"Among the several multigene families codified by the genome of T. cruzi, the TcTASV family was the latest discovered. The  TcTASV (Trypomastigote, Alanine, Serine, Valine) family is composed of ,40 members, with conserved carboxi- and aminotermini  but with a variable central core. According to the length and sequence of the central region the family is split into 3  subfamilies. The TcTASV family is conserved in the genomes of ? at least ? lineages TcI and TcVI and has no orthologues in  other trypanosomatids. In the present work we focus on the study of the TcTASV-C subfamily, composed by 16 genes in the  CL Brener strain. We determined that TcTASV-C is preferentially expressed in trypomastigotes, but it is not a major  component of the parasite. Both immunoflourescence and flow cytometry experiments indicated that TcTASV-C has a clonal  expression, i.e. it is not expressed by all the parasites of a certain population at the same time. We also determined that  TcTASV-C is phosphorylated and glycosylated. TASV-C is attached to the parasite surface by a GPI anchor and is shed  spontaneously into the medium. About 30% of sera from infected hosts reacted with TcTASV-C, confirming its exposition to  the immune system. Its superficial localization and secretory nature suggest a possible role in host-parasite interactions","palabras_clave":"Trypanosoma cruzi|TRIPOMASTIGOTE|SURFACE PROTEIN|TcTASV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64133,"idioma_id":5,"titulo":"A propósito del neotipo de Panochthus tuberculatus (Owen, 1845) (Mammalia, Xenarthra, Glyptodontia)","resumen":"In 1845, Richard Owen named Glyptodon tuberculatus erected on two carapace fragments. Later, Burmeister gave identity to that species through more detailed descriptions and established that it should pertain to the new genus Panochthus. In this way, the descriptions, figures, and photographs published by Burmeister, mainly in 1870, were consistently followed by other authors when discussing or listing Panochthus tuberculatus. As the holotype of the latter species was lost, Zamorano and collaborators assigned a neotype for it, identified as MLP 16-29. For these authors, the elements included in MLP 16-29, are attributable to a single individual and comprise an exo and endoskeleton from Tapalqué (Buenos Aires Province, Argentina). However, a detailed historical study of these elements incorporated to the neotype and a detailed comparative anatomical study of them, shows that under the collection number MLP 16-29 more than one individual is represented. Finally, some of these same anatomical elements do not match the characteristics traditionally described for P. tuberculatus. Consequently, the neotype proposed by Zamorano and collaborators is not available under the current rules of the International Code of Zoological Nomenclature.","palabras_clave":"Glyptodontia|Panochthus tuberculatus|neotype"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64134,"idioma_id":4,"titulo":"Sodium molybdate prevents hypertension and vascular prostanoid imbalance in fructose overloaded rats","resumen":"Sodium molybdate prevents hypertension and vascular prostanoid imbalance in fructose-overloaded rats.Peredo HA1, Andrade V, Donoso AS, Lee HJ, Puyó AM.Author informationAbstract(1) Fructose (F) overload produces elevated blood pressure (BP), hyperglycaemia, hypertriglyceridemia and insulin resistance, resembling human metabolic syndrome. Previously, we found altered vascular prostanoid (PR) production in this model. (2) Sodium molybdate (Mo), as well as sodium tungstate, causes insulin-like effects and normalizes plasma glucose levels in streptozotocin-treated diabetic rats. We studied the effects of Mo on BP, metabolic parameters and release of PR from the mesenteric vascular bed (MVB) in F-overloaded rats. (3) Four groups of male Sprague-Dawley rats were analysed: Control, tap water to drink; F, F solution 10% W\/V to drink; CMo, Mo 100 mg kg day(-1) and FMo, both treatments. After 9 weeks, the animals were killed and MVBs removed and the released PRs measured. (4) F increased BP, glycemia, triglyceridemia and insulinemia. Mo treatment prevented the increases in BP and glycemia, but did not modify triglyceridemia or insulinemia. In addition, Mo decreased BP in controls. (5) Prostaglandins (PG) F2 alpha and E2, PG 6-ketoF1 alpha and thromboxane (TX) B2 , as well as inactive metabolites of prostacyclin (PGI2 ) and TXA2 were detected. F decreased the production of vasodilator PRs PGI2 and PGE2 in MVB. Mo prevented these alterations and increased PGE2 in controls. Vasoconstrict or PRs PGF2 alpha and TXA2 release was not modified. (6) Mo treatment, beyond its known lowering effect on glycemia, prevents the reduction in the vascular release of vasodilator PR observed in this model. This could be one of the mechanisms by which Mo avoids the increase in BP caused by F overload in the rat.© 2013 John Wiley & Sons Ltd.KEYWORDS:fructose; hypertension; metabolic syndrome; molybdate; prostanoidsPMID: 23906370 [PubMed - indexed for MEDLINE]","palabras_clave":"SODIUM MOLYBDATE|METABOLIC SYNDROME|PROSTANOIDS|FRUCTOSE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64135,"idioma_id":4,"titulo":"Germination, radial growth and virulence to boll weevil of entomopathogenic fungi at different temperatures","resumen":"The effect of temperature on germination, radial growth and virulence of four B. bassiana and M. anisopliae isolates virulent to the boll weevil, Anthonomus grandis, were determined at different temperatures. The optimum temperature for germination and radial growth was 27 ºC for both fungi species. M. anisopliae isolates managed to germinate at higher temperatures, while B. bassiana isolates failed to germinate at 35 ºC. With regard to radial growth rate, the B. bassiana isolates grew faster at lower temperatures, whereas the M. anisopliae ones grew faster at higher temperatures. At 35 ºC only M. anisopliae isolates managed to grow. None of the isolates of both species grew at 38 ºC. The virulence against boll weevil decreased at 35 ºC for B. bassiana. For M. anisopliae isolates the median survival time increased at suboptimum temperatures, but the final mortality percentage remained the same. In conclusion, it is important to evaluate the tolerance at higher temperatures when choosing an isolate for development as a microbial control agent. Based on the temperature tolerance, M. anisopliae may be a possible candidate for a boll weevil control, and therefore it could be included in an Integrated Pest Management program on cotton crops. ","palabras_clave":"Beauveria bassiana|Metarhizium anisopliae|thermotolerance|Anthonomus grandis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64136,"idioma_id":4,"titulo":"Structure and genetic diversity of Three Calibrachoa Caesia Populations by ISSR Markers","resumen":"Calibrachoa caesia is one of thirteen native species of the genus Calibrachoa Cerv. ex La Lave et Lexarza (Solanaceae) growing in Argentina. The genus has a high ornamental value due to the morphological variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from populations of three departments in the Province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by Inter simple sequence repeat (ISSR) markers. Thirteen ISSR primers produced reproducible bands, with 652 amplified and 97.4% polymorphism. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá populations, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The analysis of molecular variance (AMOVA) test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations, showing a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs.","palabras_clave":"ANCHORED|MICROSATELLITES|MOLECULAR|MARKERS|ORNAMENTAL|PLANTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64137,"idioma_id":4,"titulo":"Innate immune responses to Proteus mirabilis flagellin in the urinary tract","resumen":"Flagella are bacterial virulence factors allowing microorganisms to move over surfaces. Flagellin, the structural component of flagella, is sensed by the host via Toll and NOD-like receptors and triggers pro-inflammatory responses. The use of Toll-like receptors agonists to modulate innate immune responses has aroused great interest as an alternative to improve the treatment of diverse infectious diseases. Proteus mirabilis is a Gram negative bacterium that causes urinary tract infections in humans. In the present work we used different approaches to study the ability of P. mirabilis flagellin to induce an innate immune response. We demonstrated that P. mirabilis flagellin has the ability to induce pro-inflammatory chemokines expression in T24 bladder cultures cells and in the mouse bladder after instillation. It was evidenced also that flagellin from different P. mirabilis strains differed in their capacity to induce an innate immune response in the CacoCCL20-Luc system. Also, flagellin elicited inflammation, with recruitment of leukocytes to the bladder epithelium. Flagellin instillation before an experimental P. mirabilis infection showed that the inflammatory response due to flagellin did not help to clear the infection but favored bacterial colonization. Thus, induction of inflammatory response in the bladder did not contribute to P. mirabilis infection neutralization.","palabras_clave":"PROTEUS MIRABILIS| UTI|FLAGELLIN|INNATE IMMUNE RESPONSE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64138,"idioma_id":5,"titulo":"Videojuegos y diversidad: construyendo una comunidad de  práctica en el aula","resumen":"En el presente trabajo mostraremos como los videojuegos comerciales utilizados como recursos educativos, pueden generar en el aula una comunidad de práctica (Lave & Wenger, 1991, Wenger, 1998, Wenger, White & Smith, 2010) modificando las relaciones entre alumnado y profesor y entre alumnos entre sí   Basándonos en una investigación de corte etnográfico y cualitativo (Denzin & Lincoln, 2011; Lacasa & Reina, 2004) analizaremos un taller realizado en un aula de 3º ESO Diversificación donde trabajamos con Sim City Creator. Puntualmente, presentaremos ejemplos de las interacciones generadas entre el alumnado y el profesor, que dan cuenta de cómo el contexto del aula se ha modificado gracias a la introducción del videojuego, generando interacciones diferentes entre sus participantes. Nuestros resultados muestran como el profesor asume el rol de guía, ofreciendo los andamiajes necesarios (Bruner, 1975; Wood, Bruner & Ross, 1976) para avanzar en el juego. Por su parte, los alumnos asumen roles activos en este proceso, ayudándose unos a   otros a adquirir mayor control sobre la tarea y un mayor dominio sobre las reglas del juego. Este tipo de uso de las tecnologías en contextos de atención a la diversidad resulta novedoso y responde a una perspectiva sociocultural de atención a la diversidad (Méndez, Lacasa & Matusov, 2008).","palabras_clave":"VIDEOJUEGOS COMERCIALES|COMUNIDAD DE PRACTICA|DIVERSIDAD|ANDAMIAJE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64139,"idioma_id":4,"titulo":"A strawberry disease caused by Acremonium strictum","resumen":"We report Acremonium strictum as the causal agent of a new disease in strawberry plants (Fragaria x ananassa Duch.) in the Northwest of Argentina. Both the structure of conidiophores and the sequence spanning the internal transcribed spacers 1 and 2 (ITS1 and ITS2) of the nuclear ribosomal DNA (rDNA) allowed confirming the affiliation of the isolate, corresponding to A. strictum. An analysis of symptoms and lesions caused by the strain of A. strictum in susceptible cultivars showed that the typical symptoms are as follows: in an early stage, small necrotic light-brown spots in leaves and petioles increase in number and size as the disease progresses; in a more advanced stage, dark necrotic areas expand over petioles and leaves causing strangulation of petioles and the plant wilt. Crown rot was not observed even at a very advanced stage of the disease.","palabras_clave":"Anthracnose|Fragaria x ananassa|Acremonium|Colletotrichum"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64140,"idioma_id":5,"titulo":"Retos en la enseñanza de la ciencia: Spore y la teoría de la evolución","resumen":"En este artículo analizamos el papel de un videojuego como recurso educativo para aprender y desarrollar el razonamiento científico en un aula de ciencias. El objetivo es generar entornos que permitan conectar el contexto escolar con el cotidiano, y viceversa, a partir de actividades que a su vez, motiven al alumnado. A nivel metodológico hemos trabajado desde una perspectiva etnográfica en las aulas de un Instituto de Educación Secundaria con un grupo de 22 estudiantes de último curso (15-17 años). Utilizamos el videojuego Spore en el marco de la asignatura de Biología, para trabajar contenidos relacionados con la teoría de la evolución. A partir de la experiencia desarrollada, mostramos cómo el videojuego Spore permite desarrollar habilidades de   pensamiento relacionadas con el análisis, evaluación y crítica de los contenidos de la asignatura de   Biología desarrollando su propia perspectiva y alcanzando una madurez crítica, a la vez que desarrollan competencias relacionadas con la capacidad de manejar información de forma activa, extraer conclusiones y resolver problemas de forma creativa. En todo este proceso es clave el papel de la maestra, ya que genera contextos de aprendizaje guiando a los estudiantes en su proceso de reflexión.","palabras_clave":"VIDEOGAME|SPORE|PENSAMIENTO CRITICO|ENSEÑANZA Y APRENDIZAJE DE LAS CIENCIAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64141,"idioma_id":5,"titulo":"El servicio doméstico en Argentina.  Particularidades y desafíos de un sector relegado (2004-2012)","resumen":"Las trabajadoras del servicio doméstico en hogares particulares representan el 25% del total de los asalariados informales en Argentina. La elevada precariedad del vínculo laboral que exhibe el sector junto con el bajo nivel educativo reflejan la vulnerabilidad socioeconómica de este segmento de la población. Tales características persisten aun en un contexto de recuperación económica como el que experimentó Argentina durante el siglo veintiuno. Ello alerta acerca de la existencia de un núcleo duro de informalidad que se mostraría insensible a los avatares del ciclo económico. Con el fin de contribuir a la explicación de tal fenómeno, el presente artículo expone algunas características sociodemográficas y normativas del empleo en servicio doméstico en Argentina durante el período 2004 a 2012.","palabras_clave":"SERVICIO DOMÉSTICO|EMPLEO|PRECARIEDAD LABORAL|ACTIVIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64142,"idioma_id":5,"titulo":"Indigentes urbanos: entre la estigmatización  y la exclusión social en la ciudad de Buenos Aires","resumen":"El estudio aquí planteado ilustra sobre la exclusión social a la que están expuestos los indigentes urbanos en Buenos Aires. El artículo se compone de dos apartados. En el primero de ellos se resumen algunos aspectos relevantes de la literatura especializada sobre la exclusión social y el funcionamiento del mercado de trabajo en Argentina en el pasado reciente. El segundo sintetiza los resultados del trabajo de campo efectuado. Éste se basó en la realización de 190 entrevistas a varones adultos residentes en hogares y paradores de la ciudad de Buenos Aires. La investigación indagó acerca de las percepciones de los entrevistados respecto de su situación de vulnerabilidad laboral y procuró rastrear los factores que determinan el tipo de inserción que logran en la actividad económica, entre ellos, la incidencia de las credenciales educativas. Se pudo constatar que la gran mayoría de los indigentes urbanos ha venido exhibiendo trayectorias laborales caracterizadas por la alternancia entre precariedad ocupacional ?desocupación abierta?, desaliento y retiro de la actividad económica. Tal dinámica parece ser insensible a los avatares del ciclo económico.","palabras_clave":"INDIGENTES|INFORMALIDAD|EXCLUSIÓN SOCIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64143,"idioma_id":5,"titulo":"ENFERMEDADES INFLAMATORIAS NO INFECCIOSAS ENCEFALICAS: REVISIÓN BIBLIOGRÁFICA","resumen":"Las enfermedades inflamatorias del sistemanervioso central (SNC) son un grupo de trastornosinflamatorios que afectan el encéfalo y\/o lamédula espinal en ausencia de causas infecciosas.1 En las últimas décadas, se han identificadovarias patologías inflamatorias de este tipo en diferentesrazas de perros, como el Pug, el Maltésy el Terrier de Yorkshire, entre otros, que presentansíndromes específicos. Todos estos síndromestienen algunos componentes en común, como eltipo de infiltrado celular, y otras característicasque son específicas de cada raza, como la localizaciónprincipal de las lesiones.","palabras_clave":"ENFERMEDADES INFLAMATORIAS|ENFERMEDADES INMUNOMEDIADAS|PERROS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64144,"idioma_id":5,"titulo":"Campus Virtual y Facebook en el ámbito universitario. ¿Enemigos o aliados en los procesos de enseñanza y aprendizaje?","resumen":"ABSTRACT (Virtual Campus and Facebook in the university. Enemies or Allies in theteaching and learning processes?)Our students live in a new era of communication. For many years chemists have used technologyfor teaching and learning at different levels of education. The use of virtual platforms is avaluable educational tool. The aim of this paper is to analyze the use of virtual platforms forstudents enrolled in the Organic Chemistry II course, of the careers of Pharmacy and Biochemistry,University of Buenos Aires. We have compared the use of the Virtual Campus andFacebook groups. A sample of 110 randomly selected students participated in this research.The 100% of students were registered on the campus of Organic Chemistry II and the 80%was a member of a related Facebook group. The 90% of the students used some of these technologicaltools as a means to support their questions. Moreover, 67% of respondents preferFacebook as a means of communication. The 64% of students felt that Facebook was themeans by which they obtained a rapid response. The results indicate that students use virtualplatforms as a support tool in the learning process.","palabras_clave":"UNDERGRADUATE STUDENTS|ORGANIC CHEMISTRY|FACEBOOK|CAMPUS VIRTUAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64145,"idioma_id":4,"titulo":"Protein signatures that promote operator selectivity among paralog MerR monovalent metal-ion regulators","resumen":"Calification SCImago-Q1, IF(2013): 4.6. Two paralog transcriptional regulators of the  MerR family, CueR and GolS, are  responsible for monovalent metal ion sensing and resistance Salmonella enterica. Although similar in  sequence and also in their target binding sites these proteins differ in signal  detection, and in the set of  target genes they control Recently, we demonstrated that selective promoter recognition depends on the presence of specific bases located at positions 3?  and 3 within the operators they interact with. Here, we identify the amino acid residues within the  N-terminal DNA-binding domain of these sensor proteins that are directly  involved in operator discrimination. We demonstrate that a methionine residue  at position 16 of GolS, absolutely conserved among GolS-like proteins, but absent  in all CueR-like xenologs, is key to selectively recognize operators that  harbor the distinctive GolS-operator signature, while residue at position 19 finely  tunes the regulator\/operator interaction. Furthermore, swapping these residues  switches the set of genes recognized by these transcription factors. These  results indicate that co-evolution  of a regulator and its cognate operators within the bacterial cell provides the  conditions to avoid cross-recognition and the proper response to metal injury.","palabras_clave":"REGULACIÓN TRANSCRIPCIONAL|ESPECIFICIDAD DE OPERADOR|GOLS\/CUER|SENSOR DE METALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64146,"idioma_id":4,"titulo":"Inhibition behavior on alkaline phosphatase activity, antibacterial and   antioxidant activities of ternary methimazole-phenanthroline-copper(II)   complex","resumen":"Methimazole (MeimzH), a sulfur containing compound, is an antithyroid drug commonly used to treatGraves? disease acting as an inhibitor of the enzyme thyroid peroxidase. A new ternary [Cu(MeimzH)2(phen)(H2O)2]Cl2 (Cu-Met-phen) complex of this drug containing also phenanthroline as a second ligand(phen) was synthesized and characterized by elemental analysis, dissolution behavior, thermogravimetricanalysis, UV?Vis, diffuse reflectance, FTIR and EPR spectroscopies. As it is previously reported, the binary[Cu(MeimzH)2(H2O)2](NO3)2H2O (Cu-Met) complex can act as an inhibitor of alkaline phosphatase(ALP). In this work, the inhibitory effect of methimazole, phenanthroline, [Cu(phen)2Cl]Cl (Cu-phen)and the new ternary complex has also been investigated and compared with Cu-Met together within vitro tests of susceptibility against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus,Staphylococcus epidermidis and Enterococcus faecalis bacteria. To our knowledge, the antibacterial activityof methimazole is determined for the first time. For the ternary complex, the results show that the coordinationof the drug increased its antibacterial effect especially against E. faecalis demonstrating a strongeffect against S. aureus and S. epidermidis. The assays also indicate that in same strains the new complexshow better activity than their ligands. The ability for the inhibition of alkaline phosphatase (ALP) waslower than that of the binary complex (Cu-Met) probably due to the absence of labile ligands aroundthe metal center. Moreover, the antioxidant activity of the complexes decreases as follows: Cu-Met > Cu-Phen > Cu-Met-Phen.","palabras_clave":"methimazole|phenantrholine|Copper(II)|SOD activity|ALP inhibition|antibacterial activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64147,"idioma_id":4,"titulo":"Lake fluctuations, plant productivity, and long term variability in high elevation tropical Andean ecosystems","resumen":"Despite the large scientific and conservation value of tropical very high elevation  Andean ecosystems (above 4500 masl) little is known about environmental trends and  ranges of variability. We combined satellite image analyses and dendrochronological  methods to quantify changes in water balance and plant productivity of these ecosystems in northern Argentina and southern Bolivia through assessments of lake level fluctuation combined with instrumental climatic records at lower elevation; and to assess the relationships between water balance and two indices of plant productivity: tree ring width of the treelet Polylepis tarapacana and seasonal patterns of vegetation 'greenness' (EVI) derived from satellite images. Between 1986 and 2007, inter-annual lake fluctuations were positively correlated with regional precipitation and de Martonne?s aridity index between, showing a decreasing trend during the period. Changes in lake size were correlated with radial growth of P. tarapacana, and with MODIS-derived annual EVI between 2001-2010; thus indicating that water balance has a significant effect on ecosystem functioning. A long term tree ring chronology indicated that recent trends of decreasing plant productivity and lakes size are still within the range of variability of the past 400 years, but such ranges may be exceeded in the coming decades if the trend persists.","palabras_clave":"Lake Fluctuations|Puna|Plant Productivity|Tree Rings"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64148,"idioma_id":5,"titulo":"SINDROME SELAR Y PARASELAR: UNA NUEVA MONONEUROPATÍA MÚLTIPLE BILATERAL EN PERROS Y GATOS","resumen":"La región selar y paraselar (SPS) contiene formaciones óseas, vasculares, nervios somáticos y autónomos, la hipófisis y las estructuras meníngeas relacionadas que la convierte en asiento de diversas patologías neurológicas, oftalmológicas o endocrinológicas.  El objetivo de este trabajo es describir la anatomía de la región SPS y comunicar un cuadro clínico observado en un perro y en 2 gatos consistente en una mononeuropatía múltiple que involucra diversos NC en su recorrido en relación a esta región que, por sus características, constituye un síndrome particular.  En nuestro conocimiento esta es la primera descripción de un síndrome que afecte a perros y gatos y que pueda comprometer simultáneamente uno o más de los NC II, III, IV, los 3 nervios que forman el V (oftálmico, maxilar y mandibular) y VI, a la glándula hipófisis y sus cubiertas meníngeas, y al tronco encefálico (diencéfalo).","palabras_clave":"REGION SELAR Y PARASELAR|SINDROMES NEUROOFTALMICOS|SINDROME DE SENO CAVERNOSO|HIPOFISIS|NERVIO MANDIBULAR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64149,"idioma_id":4,"titulo":"Synthesis, characterization and magnetic behavior of Co\/MCM-41 nano-composites","resumen":"Synthesis, structure and magnetic properties of Co\/MCM-41 as magnetic nano-composites have been investigated. Mesoporous materials with different degrees of metal loading were prepared by wet impregnation and characterized by ICP, XRD, N2 adsorption, UV?vis DRS, TPR and EPMA-EDS. Cobalt oxide clusters and Co3O4 nano-particles could be confined inside the mesopores of MCM-41, being this fact favored by the Co loading increasing. In addition, larger crystals of Co3O4 detectable by XRD also grow on the surface when the Co loading is enhanced. The magnetic characterization was performed in a SQUID magnetometer using a maximum magnetic applied field µ0Ha=1 T. While the samples with the higher Co loadings showed a behavior typically paramagnetic, a superparamagnetic contribution is more notorious for lower loadings, suggesting high Co species dispersion.","palabras_clave":"MCM-41, |Impregnation, Co species |Co species |Superparamagnetism"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64150,"idioma_id":4,"titulo":"Subfield management class delineation using cluster analysis from spatial principal components of soil variables","resumen":"Understanding spatial variation within a field is essential for site-specific crop management, which requires the delineation of management areas. Several soil and terrain variables are used to classify the field points into classes. Fuzzy k-means cluster analysis is a widely used tool to delineate management classes in the multivariate context. However, this clustering method does not consider the presence of spatial correlations in the data. The MULTISPATI-PCA algorithm is an extension of principal component analysis that considers spatial autocorrelation in the original variables to produce synthetic variables. We propose and illustrate the implementation of a new method (KM-sPC) for subfield management class delineation based on the joint use of MULTISPATI-PCA and fuzzy k-means cluster. To assess the performance of KM-sPC, we performed clustering of the original soil variables and of both spatial and classical principal components on three field data sets. KM-sPC algorithm improved the non-spatial clustering in the formation of within-field management classes. Mapping of KM-sPC classification shows a more contiguous zoning. KM-sPC showed the highest yield differences between delineated classes and the smallest within-class yield variance.","palabras_clave":"MULTISPATI_PCA|precision agriculture|fuzzy k-means"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64151,"idioma_id":5,"titulo":"Emplazamiento del cuerpo subvolcánico laguna Amarga y su relación con las estructuras tectónicas andinas,  sur de la provincia de Mendoza","resumen":"Resumen  El cuerpo sub-volcánico Laguna Amarga se encuentra ubicado en la Cordillera Principal, entre los ríos Diamante y Atuel,  aproximadamente a los 34°45? de latitud sur y 69°32? de longitud oeste, provincia de Mendoza. Este estudio refleja la forma y  modo de emplazamiento del mismo, que se asemeja a un grupo de filones capa conectado con un cuerpo lacolítico emplazado  a través de un sistema de fallas. El cuerpo Laguna Amarga forma parte del magmatismo Neógeno en el sur de Mendoza, y está  relacionado con el ciclo magmático de arco Huincán durante el Mioceno medio a tardío (13 a 5 Ma). A partir de un análisis  petrológico-geoquímico se establece que el cuerpo está constituido, en su mayoría, por rocas de composiciones andesíticas, que  muestran características mineralógicas y texturales similares tanto en la parte central del cuerpo lacolítico como en los filones  capa. Estas rocas son típicas de arco magmático, por ende su emplazamiento está directamente relacionado con la subducción de  la placa pacífica. El cuerpo de Laguna Amarga presenta una geometría que está directamente relacionada con el emplazamiento  del magma a través de sistemas de fallas que son utilizados como canales de alimentación. Estos cuerpos se intruyen durante la  compresión andina contemporáneamente con los sistemas de fallas que se propagan hacia el antepaís.  Palabras clave: Lacolito, ciclo eruptivo Huincán, faja plegada y corrida de Malargüe, Cordillera Principal.","palabras_clave":"LACOLITO|CICLO ERUPTIVO HUINCAN|FAJA CORRIDA Y PLEGADA MALARGUE|CORDILLERA PRINCIPAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64152,"idioma_id":4,"titulo":"Correlation between DAS-28 and neopterin as a biochemical marker of immune system activation in early rheumatoid arthritis","resumen":"Objective: The objective of this study was to evaluate neopterin plasma concentrations in patients with early Rheumatoid Arthritis (RA) and correlate them with disease activity. Methods: This is a 28-month prospective study carried out on 65 individuals. There were 27 patients with early RA and 38 healthy volunteers as control group. Enzyme immunoassay was used to measure concentrations of neopterin and anti-cyclic citrullinated peptide (CCP) antibodies. Rheumatoid factor (RF) and C-reactive protein (CRP) were measured turbidimetrically, and antinuclear antibodies (ANA) were detected by immunofluorescence. Patients with early RA disease activity were divided into 4 groups according to DAS-28 criteria. Neopterin concentrations in RA patients were compared to conventional RA diagnostic serological markers. Results: Healthy volunteers had a mean neopterin concentration of 5.63 ± 0.38 [1.36\u20139.93] nmol. L&#8722; 1. A statistically significant elevation of neopterin mean concentration was found on early RA patients: mean value of 8.92 ± 0.93 [3.94\u201328.3] nmol. L&#8722; 1 (p < 0.001). Pearson product moment correlation suggests a correlation between neopterin concentrations and DAS-28 (r = 0.208, p = 0.065). The analysis of the mean values grouped according with the DAS-28 criteria showed a correspondence between these means, with a Pearson correlation coefficient r = 0.979, p = 0.021. CRP concentrations also showed a similar trend. Anti-CCP antibodies and RF revealed a positive relationship with RA activity. Such a correlation was not found with ANA results. Conclusions: The elevation of plasma neopterin concentrations in early RA patients may indicate stimulation of immune response. Good correlation between neopterin concentrations and DAS-28 may facilitate assessing disease activity.","palabras_clave":"REUMATHOID ARTHRITIS|IMMUNE ACTIVATION|NEOPTERIN|DAS-28|RHEUMATOID FACTOR|C-REACTIVE PROTEIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64153,"idioma_id":5,"titulo":"MIELOPATIA DEGENERATIVA: ESTADO ACTUAL DEL CONOCIMIENTO.","resumen":"La Mielopatía Degenerativa canina (MD) es una enfermedad neurodegenerativa de comienzo tardío, diagnosticada  inicialmente en el Pastor Alemán, pero que afecta muchas otras razas. Se manifiesta como un desorden  medular en los perros adultos, de inicio insidioso y curso lentamente progresivo.  Muchos estudios consideraron la posible etiología de MD sin poder esclarecer sus causas. Investigaciones  recientes identificaron como causa probable una mutación del gen SOD1 que codifica la Superóxido Dismutasa  1, lo que implica que MD es un potencial ortólogo de la Esclerosis Lateral Amiotrófica (ELA) del ser humano.  La distribución de las lesiones y la progresión clínica de MD son similares a los de ciertos tipos de ELA,  con un inicio caracterizado por signos de motoneurona superior, con lesiones predominantes en la médula de  la región torácica, con progresión a signos de motoneurona inferior, que se vuelven evidentes en los estadios  tardíos de la enfermedad.  Antes de establecer una clara equivalencia entre DM y ELA, deben explicarse algunas diferencias entre  ambas enfermedades. Por ejemplo, que en MD la axonopatía es difusa (y no restringida al cordón lateral, como  en ELA) e involucra a los tractos sensitivos y motores y que, salvo comunicaciones aisladas, no hay evidencia  de lesión neuronal en cerebro ni en tronco encefálico.  Mientras tanto, los perros con MD son potenciales modelos animales para ELA, que pueden ser usados  para investigar los procesos subyacentes de la degeneración de la motoneurona, mapear los loci modificadores  e identificar factores ambientales que influyen en la severidad de la enfermedad.","palabras_clave":"MIELOPATIA DEGENERATIVA|PASTOR ALEMAN|MUTACION SOD1|ESCLEROSIS LATERAL AMIOTROFICA|ENFERMEDAD DE MOTONEURONA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64154,"idioma_id":4,"titulo":"Antigenicity and Immunogenicity of Rotavirus VP6 Protein Expressed on the Surface of Lactococcus lactis","resumen":"Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.","palabras_clave":"ROTAVIRUS|L. LACTIS|VP6"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64155,"idioma_id":5,"titulo":"Diseño y operación de tres modalidades de riego por goteo de tomate y sus respuestas productivas","resumen":"Fueron diseñados y operados sistemas de  riego por goteo superficial y subterráneo para  comparar la respuesta productiva de tomate  conducido bajo cubierta. Se realizó un ensayo  trasplantando en camellones dos plantines.m-2.  Los tratamientos fueron: T1) goteo subterráneo a 12,5  cm de profundidad, T2) goteo subterráneo a 25 cm de  profundidad y T3) goteo superficial. Se estimó la  humedad volumétrica del suelo Wv con sensores  Decagon EC-5, instalados a 5, 10, 20 y 30 cm de  profundidad y a 0, 15 y 30 cm desde el centro del  camellón.  Se registró el peso total y el número de tomates por  planta y el peso promedio de frutos. La distribución  de Wv fue diferente entre tratamientos. El T3 alcanzó  un promedio de Wv óptima del 21,6% en el estrato de  0 a 20 cm de profundidad, el T1 del 22,8% a los 15-30  cm y el T2 del 25,3% a los 10-30 cm.  Las áreas con rango óptimo de humedad de T1 y T2  superaron a T3 un 14 y 42 %, respectivamente. T2 y  T3 difirieron significativamente en el rendimiento por  planta, atribuible a un mayor peso promedio de frutos,  pero no hubo diferencias significativas en el número  de frutos por planta.","palabras_clave":"goteo subterráneo y superficial,|distribución de humedad,|tomate bajo cubierta| rendimientos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64156,"idioma_id":5,"titulo":"Respuesta productiva del tomate (Solanum lycopersicum L.) bajo cubierta a la distribución de la humedad generada por riego por goteo subterráneo y superficial","resumen":"Para evaluar la respuesta del tomate cultivado bajo cubierta al riego por goteo superficial y subterráneo, se propusieron 3 tratamientos: T1) goteo subterráneo a 12,5 cm de profundidad, T2) goteo subterráneo a 25 cm de profundidad y T3) goteo superficial, regados por dos cintas Aqua-TraXX espaciadas 20 cm, con emisores cada 0,2 m. Se registró la humedad volumétrica Wv dentro del camellón con sensores instalados a 5; 10; 20 y 30 cm de profundidad y a 0, 15 y 30 cm de distancia al lomo. Se analizó la variancia factorial general de los datos y aplicaron técnicas de comparación múltiple de medias observadas a posteriori, de Tukey y Bonferroni. Bajo un diseño estadístico enteramente al azar con 10 repeticiones, se registraron el peso y número de tomates por planta y el peso medio de frutos, analizando la varianza y comparando los promedios con Tukey para rendimiento y peso medio de fruto, y con prueba no paramétrica de Kruskall Wallis para número de frutos. La distribución de la Wv fue significativamente diferente entre tratamientos, T2 generó el mayor volumen de bulbo húmedo con Wv óptima, superando un 72% a T3 y un 40% a T1. El rendimiento de tomates por planta de T2 se diferenció significativamente de T3, atribuible al incremento del peso medio de frutos, sin observarse diferencias significativas en el número de frutos por planta, aunque clasificados por categorías comerciales, T2 obtuvo un 79% de tomates de primera, seguido por T1 (61,5%) y T3 (56,6%). Resultaron altamente correlacionados los rendimientos totales y pesos medios de tomates con las profundidades de goteo","palabras_clave":"riego localizado, , ,| bulbos húmedos, |tomate|rendimiento|humedad óptima."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64157,"idioma_id":4,"titulo":"Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea","resumen":"Anisakids use invertebrates as paratenic and\/or intermediate hosts as a basic feature of larval transmission. The third-stage larva usually develops in invertebrates which are prey items of finfish paratenic hosts. Contracaecum larvae moult twice inside the egg and hatch as free third-stage larvae ensheathed in the second-stage larval cuticle. Copepods act as paratenic or obligatory hosts, usually ingesting these free L3 larvae, and fish act as intermediate\/paratenic or metaparatenic hosts preying on infected copepods. Fish-eating birds acquire L3 larvae by ingesting infected fish where they develop into the fourth-stage larvae and adults. Objectives of this work were to establish the specific correspondence between C. pelagicum L3 larvae parasitizing the anchovy Engraulis anchoita, and the adults parasitizing the Magellanic penguin Spheniscus magellanicus and the Imperial shag Phalacrocorax atriceps through the use of molecular markers; and, to evaluate the anisakid L3 larval recruitment and infection caused by ingestion of anchovy by S. magellanicus. Sixteen specimens of Contracaecum L3 larvae were analyzed from E. anchoita from Bahía Engaño, Chubut, eight adult nematodes from S. magellanicus and six adult specimens from P. atriceps both from the Valdés Peninsula, Chubut. All nematodes were sequenced for three genes: mitochondrial cytochrome oxidase 2 (mtDNA cox2), mitochondrial ribosomal RNA (rrnS), and the internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA region. Phylogenetic analyses were performed by using Maximum Parsimony (MP) analysis by PAUP. In addition, studies under SEM and LM were carried out on L3 larvae. All L3 individuals from E. anchoita, adults from S. magellanicus, and P. atriceps clustered in the same clade, well supported in the MP tree inferred from the mtDNA cox2, and rrnS gene sequences analyses. Further, the sequence alignments of L3 larvae and adults of C. pelagicum here obtained at the ITS-1 and ITS-2 regions of the rDNA matched the sequences of C. pelagicum previously deposited by us in GenBank. Nematode recruitment (Ro) was equal to 33.07 (7.20 -91.14) L3 larvae for C. pelagicum in each penguin?s meal of anchovy. The MP tree topologies obtained from mtDNA cox2 and rrnS genes demonstrated that specimens of Contracaecum L3 larvae from E. anchoita and C. pelagicum from S. magellanicus as well as from P. atriceps constitute a unique clade, well-distinct and supported from all the others formed by the Contracaecum spp. sequenced so far for these genes. Molecular markers are considered to be an effective tool to elucidate larval transmission. The Contracaecum L3 larval recruitment value showed that many worms fail to establish in the bird digestive tract, probably because they are below a critical size. Further work is needed to elucidate other factors (e.g., physiological, immunological) that control nematode populations in the penguin digestive tract.","palabras_clave":"Anisakidae|Molecular Makers|Engraulis anchoita|Spheniscus magellanicus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64158,"idioma_id":5,"titulo":"Perspectivismo, fenomenología cultural y etnografías poscoloniales: intervenciones en un diálogo sobre las corporalidades","resumen":"Proponemos un diálogo intercultural entre la fenomenología cultural y el perspectivismo amerindio. A partir de diversas escenas, analizamos la utilización de los conceptos de \u201Ccuerpo-espíritu\u201D, \u201Ccarne\u201D y \u201Camerindios\u201D en tanto categorías existenciales, en el perspectivismo amazónico y en nuestra etnografía en el Chaco argentino, planteando similitudes y diferencias. Examinaremos los alcances y limitaciones de estas categorías y, en especial, la dimensión corporal y experiencial y, por ende histórica, que éstas involucran. Discutiremos cómo el perspectivismo reproduce parte del dualismo cuerpo \u2013 espíritu, fundante del pensamiento occidental moderno; asimismo, argumentaremos que las nociones de corporalidad, ser-en-el-mundo y carne, elaboradas en la fenomenología de Merleau-Ponty, desafían este dualismo, mostrándose más adecuadas para comprender no sólo las corporalidades indígenas, sino también las no-indígenas.","palabras_clave":"Perspectivismo|Fenomenología cultural|cuerpo|tobas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64159,"idioma_id":4,"titulo":"BDNF and memory processing","resumen":"Brain-derived neurotrophic factor has emerged as one of the most   important molecules involved in memory. Its wide role in different types   of memories that depend on different structures as well as its   involvement in distinct memory stages points at BDNF as one likely   target to treat cognitive impairments and anxiety-related memory   disorders. However, regulation of BDNF expression is very complex as   well as its modes of action. Here we describe the latest research   carried out on the function of BDNF in memory to illustrate such   complexity.","palabras_clave":"BDNF|MEMORY |CONSOLIDATION|PERSISTENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64160,"idioma_id":4,"titulo":"Pharmacokinetics of Cephalexin after Intravenous and Single and Multiple Intramuscular Administration to Rabbit","resumen":"Cephalexin is a first generation cephalosporin widely used in rabbits. Its spectrum includes  Pasteurella multocida and Staphylococcus aureus. These bacteria, together with Bordetella  bronchiseptica, are the main cause of respiratory infections. Although many textbooks on rabbit  therapeutics report the use of cephalexin, including administration schedules, there are not  published papers on the pharmacokinetics of cephalexin after IV and IM administration to rabbit.  Therefore, the objective of the present study was to describe cephalexin disposition in rabbits after  intravenous and single and multiple intramuscular administrations. Three administration schedules  were studied: single IV administration (10 mg\/kg), single IM administration (10 mg\/kg) and  multiple IM administration (2.5 mg\/kg\/6). Serial blood samples were collected over a 24 h period.  Cephalexin plasma concentrations were determined by microbiological method using Kocuria  rhizophila ATCC 9341 as microorganism test. No statistical differences were observed between  routes of administration for any of the estimated PK parameters. The unique difference was  observed on bioavailability between intramuscular administration schedules. Elimination half-life  was 1.45, 1.09 and 1.91 h for the single IV, single IM and multiple IM administration, respectively.  Bioavailability after single and multiple IM administration was 47 and 97.5%, respectively. After  multiple IM administration maximum and minimum plasma concentration at steady state were 2.77  and 0.34 &#956;g\/ml, while Cmax after single IM administration was 9. 22 &#956;g\/ml.  Considering that for betalactams the PK\/PD breakpoint recommended for efficacy (T > MIC) should  be 50\u201380% and that the reported MIC for most gram-positive organisms and Pasteurella  multocida is &#8804;1.0 &#956;g\/ml, the present study demonstrates that a single IM dose of 10 mg\/kg\/24 h  is enough to maintain therapeutic concentrations for a 24 hours period. When a 2.5mg\/kg dose is  used administration every 6 hours is recommended.","palabras_clave":"Pharmacokinetics|Rabbits|Cephalexin|Ciprofloxacin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64161,"idioma_id":4,"titulo":"Sliding Mode Neuro Adaptive Control in Trajectory Tracking for Mobile Robots","resumen":"In this work a neural indirect sliding&nbsp;mode control method for mobile robots is proposed. Due to the nonholonomic property and restricted mobility, the trajectory tracking of this system has been one of the research topics for the last ten years. The proposed control structure combines a feedback linearization model, based on a kinematics nominal model, and a practical design that combines an indirect neural adaptation technique with sliding mode control to compensate the dynamics of the robot. Using an online adaptation scheme, a neural sliding mode controller is used to approximate the equivalent control in the neighbourhood of the sliding manifold. A sliding control is appended to ensure that the neural sliding mode control can achieve a stable closed-loop system for the trajectory-tracking control of a mobile robot with unknown nonlinear dynamics. The proposed design simultaneously guarantees the stability of the adaptation of the neural nets and obtains suitable equivalent control when the parameters of the robot model are unknown in advance. The robust adaptive scheme is applied to a mobile robot and shown to be able to guarantee that the output tracking error will converge to zero.","palabras_clave":"mobile robot|nonlinear systems|adaptive neural control|sliding mode control"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64162,"idioma_id":4,"titulo":"Multi-Level Determinants of Parasitic Fly Infection in Forest Passerines","resumen":"The study of myiasis is important because they may cause problems to livestock industry, public health, or wildlife conservation. The ecology of parasitic dipterans that cause myiasis is singular, as they actively seek their hosts over relatively long distances. However, studies that address the determinants of myiasis dynamics are very scarce. The genus Philornis include species that may be excellent models to study myiasis ecology, as they exclusively parasitize bird nestlings, which stay in their nests until they are fully fledged, and larvae remain at the point of entry until the parasitic stage is over, thus allowing the collection of sequential individual-level infection data from virtually all the hosts present at a particular area. Here we offer a stratified multi-level analysis of longitudinal data of Philornis torquans parasitism in replicated forest bird communities of central Argentina. Using Generalized Linear Models and Generalized Linear Mixed Models and an information theory approach for model selection, we conducted four groups of analyses, each with a different study unit, the individual, the brood, the community at a given week, and the community at a given year. The response variable was larval abundance per nestling or mean abundance per nestling. At each level, models included the variables of interest of that particular level, and also potential confounders and effect modifiers of higher levels. We found associations of large magnitude at all levels, but only few variables truly governed the dynamics of this parasite. At the individual level, the infection was determined by the species and the age of the host. The main driver of parasite abundance at the microhabitat level was the average height of the forest, and at the community level, the density of hosts and prior rainfall. This multi-level approach contributed to a better understanding of the ecology of myiasis.","palabras_clave":"Myiasis|Philornis|climate change"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64163,"idioma_id":4,"titulo":"The postcranial anatomy of Coloradisaurus brevis (Dinosauria: Sauropodomorpha) from the LAte Triassic of Argentina and its phylogenetic implications","resumen":"Basal sauropodomorphs from the Upper Triassic Los Colorados Formation of north-western Argentina have been known for several decades but most of them are only briefly described. New postrcanial remains of Coloradisaurus brevis, the most gracile sauropodomorph from this unit, are described here and evaluated within a phylogenetic context. These materials belong to a single individual and include ele- ments of the vertebral column, pectoral girdle, incomplete forelimb, pelvis and hindlimb. These elements share an auta- pomorphic feature with the type specimen of Coloradisaurus brevis and provide novel and unique features that diag- nose this taxon, such as pubic apron with semicircular cross- section and with a depression on its anterior surface, distal surface of the tibia deflected and facing posterodistally and well-developed pyramidal dorsal process of the posteromedial corner of the astragalus. Several postcranial characters of Coloradisaurus are exclusively shared with Lufengosaurus, from the Lower Jurassic of China. The inclusion of this information in two recent phylogenetic data sets depicts Coloradisaurus as closely related to Lufengosaurus and well nested within Plateosauria. Both data sets used indicate strong character support for the inclusion of Coloradisaurus and Lufengosaurus within Massospondylidae.","palabras_clave":"Sauropodomorpha|Coloradisaurus|Triassic|Phylogeny"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64164,"idioma_id":4,"titulo":"The Stimulated Innate Resistance Event in Bordetella pertussis Infection Is Dependent on Reactive Oxygen Species Production","resumen":"The exacerbated induction of&nbsp;innate&nbsp;immune responses in airways can abrogate diverse lung infections by a phenomenon known as&nbsp;stimulated&nbsp;innateresistance&nbsp;(StIR). We recently demonstrated that the enhancement of&nbsp;innate&nbsp;response activation can efficiently impair&nbsp;Bordetella&nbsp;pertussiscolonization in a Toll-like receptor 4 (TLR4)-dependent&nbsp;manner. The aim of this work was to further characterize the effect of lipopolysaccharide (LPS) on StIR and to identify the mechanisms that mediate this process. Our results showed that bacterial&nbsp;infection&nbsp;was completely abrogated in treated mice when the LPS of B.&nbsp;pertussis&nbsp;(1 &#956;g) was added before (48 h or 24 h), after (24 h), or simultaneously with the B.&nbsp;pertussis&nbsp;challenge (10(7) CFU). Moreover, we detected that LPS completely cleared bacterial&nbsp;infection&nbsp;as soon as 2 h posttreatment. This timing suggests that the observed StIR phenomenon should be mediated by fast-acting antimicrobial mechanisms. Although neutrophil recruitment was already evident at this time point, depletion assays using an anti-GR1 antibody showed that B.&nbsp;pertussis&nbsp;clearance was achieved even in the absence of neutrophils. To evaluate the possible role of free radicals in StIR, we performed animal assays using the antioxidant N-acetyl cysteine (NAC), which is known to inactivate oxidantspecies. NAC administration blocked the B.&nbsp;pertussis&nbsp;clearance induced by LPS. Nitrite concentrations were also increased in the LPS-treated mice; however, the inhibition of nitric oxide synthetases did not suppress the LPS-induced bacterial clearance. Taken together, our results show thatreactive&nbsp;oxygen&nbsp;species&nbsp;(ROS) play an essential role in the TLR4-dependent&nbsp;innate&nbsp;clearance of B.&nbsp;pertussis.","palabras_clave":"STIR|BORDETELLA PERTUSSIS|TLR4"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64165,"idioma_id":4,"titulo":"Ageing of thermosets based on tung oil\/styrene\/ divinylbenzene","resumen":"New thermoset polymers prepared by cationic co-polymerization of tung oil with styrene and\/or divinylbenzene were evaluated at different times after their preparation.  The changes in the properties were correlated with the composition of the copolymers. The action of atmospheric oxygen on the fatty acid unsaturations produced chemical changes in these polymers, which affected the properties of the cured materials. These changes were analyzed by FTIR, dynamic mechanical properties and mechanical testing. An increase in the modulus with time for all the analyzed samples was observed, as well as a large effect on the glass transition temperature and, consequently, in the shape memory properties.","palabras_clave":"tung oil|ageing|mechanical properties|oxidative polymerization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64166,"idioma_id":4,"titulo":"Variability in Abundance of Temperate Reef Fishes Estimated by Visual Census","resumen":"Identifying sources of sampling variation and quantifying their magnitude is critical to the interpretation of ecological field data. Yet, most monitoring programs of reef fish populations based on underwater visual censuses (UVC) consider only a few of the factors that may influence fish counts, such as the diver or census methodology. Recent studies, however, have drawn attention to a broader range of processes that introduce variability at different temporal scales. This study analyzes the magnitude of different sources of variation in UVCs of temperate reef fishes off Patagonia (Argentina). The variability associated with time-of-day, tidal state, and time elapsed between censuses (minutes, days, weeks and months) was quantified for censuses conducted on the five most conspicuous and common species: Pinguipes brasilianus, Pseudopercis semifasciata, Sebastes oculatus, Acanthistius patachonicus and Nemadactylus bergi. Variance components corresponding to spatial heterogeneity and to the different temporal scales were estimated using nested random models. The levels of variability estimated for the different species were related to their life history attributes and behavior. Neither time-of-day nor tidal state had a significant effect on counts, except for the influence of tide on P. brasilianus. Spatial heterogeneity was the dominant source of variance in all but one species. Among the temporal scales, the intra-annual variation was the highest component for most species due to marked seasonal fluctuations in abundance, followed by the weekly and the instantaneous variation; the daily component was not significant. The variability between censuses conducted at different tidal levels and time-of-day was similar in magnitude to the instantaneous variation, reinforcing the conclusion that stochastic variation at very short time scales is non-negligible and should be taken into account in the design of monitoring programs and experiments. The present study provides baseline information to design and interpret results from visual census programs in temperate reefs.","palabras_clave":"SAMPLING VARIATION|PATAGONIA|VARIANCE COMPONENTS|SAMPLING DESIGN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64167,"idioma_id":4,"titulo":"Enhancement of Th1 immune responses to recombinant influenza nucleoprotein by Ribi adjuvant","resumen":"A broad coverage influenza vaccine against multiple viral strains based on the viral nucleoprotein (NP) is a goal pursuedby many laboratories. If the goal is to formulate the vaccine with recombinant NP it is essential to count on adjuvantscapable of inducing cellular immunity. This work have studied the effect of the monophosphoryl lipid A andtrehalose dimycolate, known as the Ribi Adjuvant System (RAS), in the immune response induced in mice immunizedwith recombinant NP. The NP was formulated with RAS and used to immunize BALB\/c mice. Immunizations withNP-RAS increased the humoral and cellular immune responses compared to unadjuvanted NP. The predominant antibodyisotype was IgG2a, suggesting the development of a Th1 response. Analysis of the cytokines from mice immunizedwith NP-RAS showed a significant increase in the production of IFN-g and a decreased production of IL-10 andIL-4 compared to controls without RAS. These results are similar to those usually obtained using Freund?s adjuvant,known to induce Th1 and CTL responses when co-administered with purified proteins, and suggest that a similar approachmay be possible to enhance the performance of a T-cell vaccine containing NP.","palabras_clave":"INFLUENZA VIRUS|RECOMBINANT NUCLEOPROTEIN|RIBI ADYUVANT SYSTEM|TH1 IMMUNE RESPONSE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64168,"idioma_id":4,"titulo":"Viral etiology of acute respiratory infections in hospitalized and outpatient children in Buenos Aires, Argentina","resumen":"OBJECTIVES:To determine and compare the viral frequency, seasonality and clinical-demographic features in 2 groups of children (hospitalized versus outpatients) with acute respiratory infections.MATERIAL AND METHODS:A cross-sectional, descriptive study was performed from 2008 to 2010 in 620 children <6 years of age with acute respiratory infection. Respiratory samples were studied for classical respiratory viruses by immunofluorescence and for human rhinoviruses (HRV) by real-time reverse transcription polymerase chain reaction. Clinical and demographic data were recorded.RESULTS:Viral detection by immunofluorescence was 48% in 434 inpatients and 37% in 186 outpatients. Viral diagnosis increased to 83% and 62%, respectively, when testing for HRV. HRV (41%) and respiratory syncytial virus (RSV) (27%) were most common viruses identified, followed by metapneumovirus (9%), influenza A and parainfluenza (3%), adenovirus and influenza B (2%). HRV frequency was significantly higher in hospitalized patients (47%) than in outpatients (27%) (P < 0.001). Coinfection was detected in 12% of hospitalized and 4% of outpatients (P < 0.031). HRV and adenovirus circulated throughout the entire year. RSV, influenza A and B predominated in winter, whereas metapneumovirus and parainfluenza predominated in spring. Of 362 patients with bronchiolitis, 84% had a virus identified; HRV (42%) and RSV (38%) were predominant. Of 77 patients with pneumonia, 84% had a virus detected with HRV (43%) and RSV (29%) predominating.CONCLUSIONS:HRV were significant pathogens associated with bronchiolitis and pneumonia, especially in hospitalized patients. Both, HRV and coinfections, were risk factors for hospitalization. These findings support the importance of including HRV detection in children with acute respiratory infection.","palabras_clave":"respiratory viruses|rhinovirus|acute respiratory infections|real-time reverse transcription polymerase chain reaction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64169,"idioma_id":4,"titulo":"NO + NH3 reaction over polycrystalline Pt: Numerical analysis of spatio-temporal data and evidence of non  linear behavior","resumen":"&lt;!-- \/* Style Definitions *\/ p.MsoNormal, li.MsoNormal, div.MsoNormal\t{mso-style-parent:'';\tmargin:0cm;\tmargin-bottom:.0001pt;\tmso-pagination:widow-orphan;\tfont-size:12.0pt;\tmso-bidi-font-size:10.0pt;\tfont-family:'Times New Roman';\tmso-fareast-font-family:'Times New Roman';\tmso-ansi-language:ES-TRAD;}@page Section1\t{size:612.0pt 792.0pt;\tmargin:70.85pt 3.0cm 70.85pt 3.0cm;\tmso-header-margin:36.0pt;\tmso-footer-margin:36.0pt;\tmso-paper-source:0;}div.Section1\t{page:Section1;}--&gt;Surfacecatalytic reactions display under low pressure conditions non-linear featuressuch as non-stationary reaction rates and spatio-temporal patterns known asdissipative structures. We analyze experimental data from ammonia oxidationreaction over polycrystalline platinum catalyst using quantitative andqualitative nonlinearity tests, such as the Grassberger-Procaccia (GP)algorithm and Surrogate Time Series. In order to perform such analysis, localand global time series were extracted from both spatially resolved and globalmeasurements. Additionally, evidence confirming previously, proposed mechanismfor reaction system.","palabras_clave":"NO + NH3 reaction|polycrystalline Pt|non  linear behavior|numerical analysis of spatio-temporal data"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64170,"idioma_id":5,"titulo":"Indicadores de control ciudadano para el goce efectivo del derecho a la ciudad en términos de desarrollo urbano para la ciudad de Córdoba (Argentina)","resumen":"La desigualdad y fragmentación territorial exigen la co-responsabilización de todos los sectores, en un esfuerzo deliberado y organizado para lograr las transformaciones necesarias y favorecer el goce del derecho a la ciudad. Esta ponencia caracteriza el proceso de construcción colectiva de indicadores de control ciudadano, desarrollado por el Grupo de Desarrollo Urbano de la Red Ciudadana Nuestra Córdoba para la ciudad de Córdoba (Argentina). Como objetivo procura la identificación y caracterización integral de las violaciones al derecho a la ciudad, confrontando miradas y acciones que coexisten en la construcción territorial; y en base a su diagnóstico y visibilización, proponer estrategias de superación e incidencia en el ámbito de las políticas públicas. Constituye un aporte reflexivo sobre las condiciones necesarias para el efectivo cumplimiento de este derecho y al análisis político sobre los poderes y mecanismos de control en la reconfiguración de los territorios urbanos, donde el papel ciudadano resulta estratégico.","palabras_clave":"INDICADORES|CONTROL CIUDADANO|DERECHO A LA CIUDAD|DESARROLLO URBANO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64171,"idioma_id":4,"titulo":"Sertraline and its iodine product: Experimental and theoretical vibrational studies Potential in vitro anti-thyroid activity of sertraline and iodine product toxicity with respect to male Wistar rats","resumen":"Mayor depression, obsessive\u2013compulsive panic, social anxiety disorders are common diseases that areusually treated with sertraline hydrochloride which is the active ingredient of the well known drugsas Zoloft and Lustral. In this work, we presented a more complete vibrational characterization of the solidphase FT-IR spectra of Sertraline hydrochloride and its sertraline\u2013iodine product in which the conforma-tional space of the molecules was investigated performing molecular dynamic simulations within an NVTensemble. Geometrical, electronic and vibrational properties were calculated with the density functionaltheory. Comparison of the simulated spectra with the experimental spectra provides important informa-tion about the ability of the computational method to describe the vibrational modes of both molecules.In addition, for the first time we present the evaluation of anti-thyroid activity of sertraline hydrochlorideby using the Lang\u2019s method. Also, with the aim to evaluate the antidepressant effect of its iodine productwe demonstrated for this compound the toxic effect towards the male Wistar rats.","palabras_clave":"Infrared spectroscopy|UV\/Vis spectroscopy|In vitro models|sertraline"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64172,"idioma_id":4,"titulo":"S-model calculations for high-energy-electron-impact double ionization of helium","resumen":"In this paper the double ionization of helium by high-energy electron impact is studied. The corresponding four-body Schrödinger equation is transformed into a set of driven equations containing successive orders in the projectile-target interaction. The transition amplitude obtained from the asymptotic limit of the first-order solution is shown to be equivalent to the familiar first Born approximation. The first-order driven equation is solved within a generalized Sturmian approach for an S-wave (e,3e) model process with high incident energy and small momentum transfer corresponding to published measurements. Two independent numerical implementations, one using spherical and the other hyperspherical coordinates, yield mutual agreement. From our ab initio solution, the transition amplitude is extracted, and single differential cross sections are calculated and could be taken as benchmark values to test other numerical methods in a previously unexplored energy domain.","palabras_clave":"ionization|electron continuum|sturmian expansion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64173,"idioma_id":5,"titulo":"Una política social particular para la infancia en la década del ´30: el patronato y los tribunales de menores en la provincia de Mendoza","resumen":"El presente trabajo se propone analizar los orígenes y la organización  del Patronato de Menores (PM) en la provincia de Mendoza en el marco de los  cambios sociales e ideológicos de los años treinta con relación a los 'menores  delincuentes y desvalidos'. Se pretende, desde una mirada histórica e institucional,  analizar cómo el Estado abordó el problema de la delincuencia y de la pobreza  en el período de entreguerras a partir de una de las primeras instituciones  organizadas a tal fin en la   Argentina. En este sentido, se plantea el marco general en el  cual fue creado el PM, la concepción de la época, los factores que llevaron a  dicha institucionalización y la construcción de un sujeto social particular -el  menor delincuente y desvalido- sobre el cual el Estado quiere actuar.  La hipótesis que sigue este trabajo es que la creación del PM fue el  producto de los cambios ideológicos de la época que intenta colocar al Estado interventor  y dirigista como organizador de la sociedad a través de la acción  control-represiva de los sectores más conflictivos de la sociedad. La  conjunción en el tratamiento estatal de la problemática de la pobreza y la  delincuencia constituyen la representación de una nueva perspectiva dominante  en materia de política social de la época en el país.","palabras_clave":"Patronato de menores|Justicia|provincia de Mendoza|educación"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64174,"idioma_id":4,"titulo":"VISTA's view of the Sagittarius dwarf spheroidal galaxy and southern Galactic Bulge","resumen":"We present the deepest near-infrared (ZJKs) photometry yet obtained of the Sagittarius dwarf spheroidal (Sgr dSph), using Visible and Infrared Survey Telescope for Astronomy (VISTA) to survey 11 square degrees centred on its core. We list locations and ZJKs-band magnitudes for over 2.9 million sources in the field. We discuss the isolation of the Sgr dSph from the foreground and Galactic Bulge populations, identify the Sgr dSph´s horizontal branch in the near-infrared for the first time and map the density of the galaxy´s stars. We present isochrones for the Sgr dSph and Bulge populations. These are consistent with the previously reported properties of the Sgr dSph core: namely that it is dominated by a population between [Fe\/H] &#8776; -1 dex and solar, with a significant [&#945;\/Fe] versus [Fe\/H] gradient. While strong contamination from the Galactic Bulge prevents accurate measurement of the (Galactic) north side of the Sgr dSph, the dwarf galaxy can be well approximated by a roughly ovaloid projection of characteristic size 4° × 2°, beyond which the projected stellar density is less than half that of the region surrounding the core. The galaxy´s major axis is perpendicular to the Galactic Plane, as in previous studies. We find slight evidence to confirm a metallicity gradient in the Sgr dSph and use isochrones to fit a distance of 24.3 ± 2.3 kpc. We were unable to fully constrain the metallicity distribution of the Sgr dSph due to the Bulge contamination and strong correlation of [&#945;\/Fe] with metallicity; however, we find that metal-poor stars ([Fe\/H] &#8818; -1) make up &#8818;29 per cent of the Sgr dSph´s upper red giant branch population. The Bulge population is best fitted by a younger population with [Fe\/H] &#8776; 0 and [&#945;\/Fe] &#8776; 0 or slightly higher. We find no evidence for a split, peanut- or X-shaped Bulge population in this line of sight (l = 5.6° ± ?1°, b = -14.1° ± ?3°).","palabras_clave":"stars: AGB and post-AGB |stars: evolution|stars: fundamental parameters|Bstars: late type|Galaxy: bulge|galaxies: individual: Sgr dSph"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64175,"idioma_id":4,"titulo":"Greenhouse gasses emissions and energy balances of a non- vertically integrated sugar and ethanol supply chain: a case study in Argentina","resumen":"In order to address society\u2019s concerns regarding the sustainability of sugar and ethanol production anduse, this paper calculates the energy and greenhouse gasses (GHG) emissions\u2019 balances of a nonverticallyintegrated sugarcane industry in Tucumán-Argentina. The essential operations involved inthe sugar\/ethanol production cycle were taken into account. Results show that this industry generated anenergy balance of 3.4:1. GHG emissions during sugarcane production were 1824 and 2231 kg CO2eq. ha1\u2019s concerns regarding the sustainability of sugar and ethanol production anduse, this paper calculates the energy and greenhouse gasses (GHG) emissions\u2019 balances of a nonverticallyintegrated sugarcane industry in Tucumán-Argentina. The essential operations involved inthe sugar\/ethanol production cycle were taken into account. Results show that this industry generated anenergy balance of 3.4:1. GHG emissions during sugarcane production were 1824 and 2231 kg CO2eq. ha1\u2019 balances of a nonverticallyintegrated sugarcane industry in Tucumán-Argentina. The essential operations involved inthe sugar\/ethanol production cycle were taken into account. Results show that this industry generated anenergy balance of 3.4:1. GHG emissions during sugarcane production were 1824 and 2231 kg CO2eq. ha12eq. ha1year1 for low and middle to high farms\u2019 technological levels, respectively. The mill process emitted1187 kg CO2eq. ha1 year1. The main factors influencing these balances were gas-oil and nitrogen fertilizersused in the agricultural stage, natural gas consumed by the sugar mill, and sugarcane burning(only for GHG balance). The impact of ethanol use in reducing GHG emissions under the current productionscheme (6.8 Mg ha1 of sugar þ 380.9 kg ha1 of ethanol), in final blends of 95% gasoline and 5%ethanol in vehicles, is negligible. A sensitivity analysis indicates that switching to 100% bagasse used asfuel in mill\u2019s boilers, ethanol being produced directly from sugarcane juice, and a final blend of 90%gasoline and 10% ethanol, an amount of 1746 kg CO2eq. ha1 year1 of GHG emissions could be avoided.1 for low and middle to high farms\u2019 technological levels, respectively. The mill process emitted1187 kg CO2eq. ha1 year1. The main factors influencing these balances were gas-oil and nitrogen fertilizersused in the agricultural stage, natural gas consumed by the sugar mill, and sugarcane burning(only for GHG balance). The impact of ethanol use in reducing GHG emissions under the current productionscheme (6.8 Mg ha1 of sugar þ 380.9 kg ha1 of ethanol), in final blends of 95% gasoline and 5%ethanol in vehicles, is negligible. A sensitivity analysis indicates that switching to 100% bagasse used asfuel in mill\u2019s boilers, ethanol being produced directly from sugarcane juice, and a final blend of 90%gasoline and 10% ethanol, an amount of 1746 kg CO2eq. ha1 year1 of GHG emissions could be avoided.2eq. ha1 year1. The main factors influencing these balances were gas-oil and nitrogen fertilizersused in the agricultural stage, natural gas consumed by the sugar mill, and sugarcane burning(only for GHG balance). The impact of ethanol use in reducing GHG emissions under the current productionscheme (6.8 Mg ha1 of sugar þ 380.9 kg ha1 of ethanol), in final blends of 95% gasoline and 5%ethanol in vehicles, is negligible. A sensitivity analysis indicates that switching to 100% bagasse used asfuel in mill\u2019s boilers, ethanol being produced directly from sugarcane juice, and a final blend of 90%gasoline and 10% ethanol, an amount of 1746 kg CO2eq. ha1 year1 of GHG emissions could be avoided.1 of sugar þ 380.9 kg ha1 of ethanol), in final blends of 95% gasoline and 5%ethanol in vehicles, is negligible. A sensitivity analysis indicates that switching to 100% bagasse used asfuel in mill\u2019s boilers, ethanol being produced directly from sugarcane juice, and a final blend of 90%gasoline and 10% ethanol, an amount of 1746 kg CO2eq. ha1 year1 of GHG emissions could be avoided.\u2019s boilers, ethanol being produced directly from sugarcane juice, and a final blend of 90%gasoline and 10% ethanol, an amount of 1746 kg CO2eq. ha1 year1 of GHG emissions could be avoided.2eq. ha1 year1 of GHG emissions could be avoided.","palabras_clave":"Sugarcane|Bioenergy|Mitigation|Tucumán"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64176,"idioma_id":4,"titulo":"'Anchors for the Cosmic Distance Scale: the Cepheid QZ Normae in the Open Cluster NGC 6067","resumen":"Cepheids are key to establishing the cosmic distance scale. Thereforeit´s important to assess the viability of QZ Nor, V340 Nor, and GU Noras calibrators for Leavitt´s law via their purported membership in theopen cluster NGC 6067. The following suite of evidence confirms that QZNor and V340 Nor are members of NGC 6067, whereas GU Nor likely lies inthe foreground: (i) existing radial velocities for QZ Nor and V340 Noragree with that established for the cluster () to within 1 km\/s, whereasGU Nor exhibits a markedly smaller value; (ii) a steep velocity-distancegradient characterizes the sight-line toward NGC 6067, thus implyingthat objects sharing common velocities are nearly equidistant; (iii) aradial profile constructed for NGC 6067 indicates that QZ Nor is withinthe cluster bounds, despite being 20´ from the cluster center; (iv) newBVJH photometry for NGC 6067 confirms the cluster liesd=1.75±0.10 kpc distant, a result that matches Wesenheitdistances computed for QZ Nor\/V340 Nor using the Benedict et al.(Astron. J. 133:1810, 2007, HST parallaxes) calibration. QZ Nor is acluster Cepheid that should be employed as a calibrator for the cosmicdistance scale.","palabras_clave":"Stars|Variables|Cepheids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64177,"idioma_id":4,"titulo":"The long bar as seen by the VVV Survey. II. Star counts","resumen":"Context. There is still some debate about the presence and the morphological properties of the long bar in the inner Galaxy.   Aims: We investigate the morphological properties of the long Galactic bar using the VVV survey extending star counts at least 3 mag deeper than 2MASS. Our study covers the relatively unexplored negative longitudes of the Galactic bar. We obtain a detailed description of the spatial distribution of star counts towards the long Galactic bar as well as to measure its parameters.   Methods: We performed star counts towards -20° < &#8467; < 0, |b|&#8804; 2° using VVV, 2MASS, and GLIMPSE data. We applied an average interstellar extinction correction. We also adjusted latitudinal profiles to obtain the centroid variation and bar thickness.   Results: We probe the structure of long Galactic bar, as well as its far edge at &#8467; &#8776; -14°. The differences between counts with and without extinction correction allow us to produce a crude extinction map showing regions with high extinction, mainly beyond the end of long Galactic bar. The latitudinal profiles show evidence of the centroid vertical variation with Galactic longitude reaching a minimum at &#8467; &#8776; -13.8°. The bar has an inclination angle &#945; = 43° ± 5° with respect to the line Sun-Galactic center. In addition, we have determined the bar parameters, such as thickness, length, and stellar distribution.","palabras_clave":"ISM: structure, dust, extinction|Galaxy: stellar content|Galaxy: structure|Galaxy: center|Galaxy: general"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64178,"idioma_id":4,"titulo":"Transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease","resumen":"              Citrus canker provoked by Xanthomonas axonopodis pv. citri is  a bacterial disease causing severe losses in all citrus-producing areas around  the world. Xanthomonas infection is  considered endemic disease in Northeast and Northwest Argentina, affecting as  much as 10% of commercial citrus plantations. There is not known natural  resistance neither in orange varieties nor in rootstocks used for grafting of  commercial cultivars. To introduce resistance to this bacterial disease, plants  of Pineapple sweet orange were transformed with a genetic constructs allowing  constitutive accumulation of dermaseptin in the apoplastic space. As compared  to non-transformed controls, the transgenic  plants showed bacterial resistance levels varying between 40-60% in  infection assays conducted with X. axonopodis pv. citri under growth chamber conditions.  ","palabras_clave":"Citricos|Cancrosis|dermaseptina|biotecnologia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64179,"idioma_id":4,"titulo":"A multi-step pathway connecting short sleep duration to daytime somnolence, reduced attention and poor academic performance. An exploratory cross-sectional study in teenagers","resumen":"Background: A multi-step causality pathway connecting short sleep duration to daytime somnolence and sleepiness leading to reduced attention and poor academic performance as the final result, can be envisaged. However this hypothesis has never been explored.  Objective: To explore consecutive correlations between sleep duration, daytime somnolence, attention levels and academic performance in a sample of school-aged teenagers.  Methods: We carried out a survey assessing sleep duration and daytime somnolence using the Pediatric Daytime Sleepiness Scale (PDSS). Sleep duration variables included weekdays? total sleep time, usual bedtimes and absolute weekday-to-weekend sleep time difference. Attention was assessed by d2 test and by Code subtest from the WISC-IV scale. Academic performance was obtained from literature and math grades. Structural Equation Modeling was used to assess the relationships between these variables, while controlling for confounding effects of other variables. Standardized Regression weights (swr) for relationships between these variables are reported.  Results: Study sample included 1194 teenagers (Mean age: 15 years; range: 13 - 17 y). Sleep duration was inversely associated with daytime somnolence (swr=-0.36 p<0.01) while sleepiness was negatively associated with attention (swr=-0.13 p<0.01). Attention scores correlated positively with academic results (swr=0.18 p<0.01). Daytime somnolence correlated negatively with academic achievements (swr=-0.16 p<0.01). The model offered an acceptable fit according to usual measures (RMSEA=0.0548, CFI=0.874, NFI=0.838). A Sobel test confirmed that short sleep duration influenced attention through daytime somnolence (p<0.02) which in turn influenced academic achievements through reduced attention (p<0.002).  Conclusions: Poor academic achievements correlated with reduced attention, which in turn was related to daytime somnolence. Somnolence correlated with short sleep duration.","palabras_clave":"SLEEP|SOMNOLENCE|ACADEMIC PERFORMANCE|TEENAGERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64180,"idioma_id":4,"titulo":"Bacteria as vitamin suppliers to their host: a gut microbiota perspective","resumen":"Food-related lactic acid bacteria (LAB) as well as human gut commensals such as bifidobacteria can de novo synthesize and supply vitamins. This is important since humans lack the biosynthetic capacity for most vitamins and these must thus be provided exogenously. Although vitamins are present in a variety of foods, deficiencies still occur, mainly due to malnutrition as a result of insufficient food intake and because of poor eating habits. Fermented milks with high levels of B-group vitamins (such as folate and riboflavin) can be produced by LAB-promoted and possibly bifidobacteria-promoted biosynthesis. Moreover, certain strains of LAB produce the complex vitamin cobalamin (or vitamin B12). In this review, fermented foods with elevated levels of B-group vitamins produced by LAB used as starter cultures will be covered. In addition, genetic abilities for vitamin biosynthesis by selected human gut commensals will be discussed.","palabras_clave":"MICROBIOTA|VITAMINS|LACTIC ACID BACTERIA|MICROBIOMA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64181,"idioma_id":4,"titulo":"Draft Genome Sequence of Enterococcus faecium Strain CRL 1879 Isolated from a Northwestern Argentinian Artisanal Cheese","resumen":"We report the draft genome sequence of the bacteriocin producer   Enterococcus faecium strain CRL 1879, isolated from a northwestern   Argentinian artisanal cheese. The draft genome sequence is composed of   73 contigs for 2,886,747&nbsp;bp, with 3,140 protein-coding genes. Six   biosynthetic clusters for bacteriocin class II production were found.   Typical virulence determinants, which have relevance in food safety,   were not present.","palabras_clave":"GENOME|ENTEROCOCCUS FAECIUM|BACTERIOCIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64182,"idioma_id":5,"titulo":"Evaluación de la actividad insecticida de tierras de diatomeas de yacimientos argentinos.","resumen":"  El uso de tierras de diatomeas (TDs) como insecticida de bajo riesgo para la salud humana y el ambiente está registrado en numerosos países para la protección de granos almacenados. Se evaluó la capacidad insecticida de TDs obtenidas de yacimientos argentinos mediante bioensayos con coleópteros que son la plaga de granos almacenados. Los resultados de estos ensayos se compararon con parámetros fisicoquímicos de las TDs (densidad aparente compactada, reducción del peso hectolítrico, adherencia al grano y pH), propuestos por Korunic (1997), como parámetros predictivos para la evaluación de la eficacia insecticida. Los resultados obtenidos por ambos métodos fueron concordantes y variaron en función del yacimiento y de la especie de insecto en estudio. La metodología descripta es un procedimiento sencillo y eficaz para efectuar una evaluación preliminar de la capacidad insecticida del material proveniente de diferentes canteras de tierra de diatomeas. Este estudio contribuirá a la evaluación económica de potenciales explotaciones de diversos yacimientos de TDs en territorio argentino ","palabras_clave":"DIATOMEAS|INSECTOS PLAGA|GRANOS ALMACENADOS|CONTROL NATURAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64183,"idioma_id":5,"titulo":"Sistemas de fenotipado para la evaluación de las respuestas al estrés salino en Lotus","resumen":"La tolerancia al estrés salino es un carácter complejo determinado por la sumatoria de los efectos de numerosos genes, cuya  identificación es necesaria para la definición de estrategias de mejoramiento. En este sentido, el uso de plantas modelo  genotipadas y la disponibilidad de métodos de fenotipado masivo son útiles para asistir a los programas de mejoramiento.  Lotus japonicus (Regel) K. Larsen es un modelo de leguminosa con nodulación determinada, que puede ser utilizada en la  identificación de determinantes genéticos en programas de mejoramiento de Lotus y otras leguminosas forrajeras. En este  trabajo, para establecer un sistema de cultivo adecuado para el fenotipado masivo, se compararon cuatro sistemas experimentales  para evaluar respuestas a estrés salino: placas, pouch, hidroponía y macetas. Los resultados obtenidos permitieron  elegir el sistema de hidroponía como el más adecuado para evaluar el estrés salino, dado que se pudieron establecer  diferencias en las respuestas fisiológicas basadas en parámetros de crecimiento y desarrollo en los genotipos Lotus japonicus  Gifu y Lotus burttii. Además, el sistema de hidroponía permitió discriminar respuestas en una población de líneas  recombinantes puras (RILs), producto del cruzamiento de Lotus japonicus Gifu x Lotus burttii. Contar con un sistema de  cultivo que permita realizar de manera precisa el fenotipado masivo de plantas, es necesario para identificar regiones del  genoma implicadas en las respuestas a estrés salino.  Palabras clave: sistemas de cultivo, hidroponía, fenotipado masivo, RILs, NaCl","palabras_clave":"FENOTIPADO|SISTEMAS DE CULTIVO|RILS|ESTRES SALINO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64184,"idioma_id":4,"titulo":"Environmental changes affecting light climate in oligotrophic mountain lakes: the Deep Chlorophyll Maxima as a sensitive variable","resumen":"North Patagonian Andean lakes from Argentina are high light\/low nutrient environments that during summer stratification exhibit the development of a Deep Chlorophyll Maxima (DCM) at the metalimnetic layers, near 1% of surface PAR irradiance.  Here, we analyse if the position of DCM change as a consequence of long time effect (global warming: glacial clay input) and short time effect (eruption: volcanic ashes).  We perform different field studies: 1- an intrannual analysis of six lakes from different basins including the data of the 2011 volcanic eruption that cause an unexpected variation in water transparency, and 2- an intralascustrine analysis in which we compared different stations in a transparency gradient of Lake Mascardi, caused by glacial clay input in one end of the gradient.  In these analyses we documented changes in the DCM depth and their relationship with different parameters. DCM development was not observed in coincidence with thermocline depth nor with nutrient distribution.  In all cases, the only significant variables were Kd 320 nm and Kd PAR. Indeed, our study showed that suspended particles (glacial clay and volcanic ashes) may play a crucial role in transparent lakes impacting lake features as phototrophic biomass distribution along the water column. The increase of suspended solid coming either from glacial clay or from volcanic ashes produced a comparable effect provoking a decrease in light and a consequently upper location in DCM development.  Thus, DCM development was a very sensitive feature to global changes as increase in temperature, that cause glacier recession, or regional changes caused by volcanic eruptions.","palabras_clave":"cambio global|irradiancia|clorofila"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64185,"idioma_id":4,"titulo":"Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: role of TGF-beta1","resumen":"Peripheral inflammation, both during the prenatal period and in adulthood, impairs adult neurogenesis. We hypothesized that, similar to other programming effects of prenatal treatments, only prenatal inflammation causes long-term consequences in adult neurogenesis and its neurogenic niche. To test this, pregnant Wistar rats were subcutaneously injected with lipopolysaccharide (LPS; 0.5mg\/kg) or saline solution every other day from gestational\/embryonic day (GD) 14 to 20. In addition adult animals were injected with a single intraperitoneal saline or LPS injection (1mg\/kg) and the effects on neurogenesis were assessed 7 days later. Alternatively, to evaluate long-term consequences of adult LPS injections, LPS (1mg\/kg) was administered peripherally to adult rats 4 times every other day, and the effects on neurogenesis were assessed 60 days later.  Prenatal and adult LPS treatments reduced adult neurogenesis and provoked specific microglial (but not astroglial) activation in the dentate gyrus (DG). However, only prenatal inflammation-mediated effects were long-lasting (at least 60 days). Moreover, these effects were specific to the DG since the Subventricular Zone (SVZ) and the Rostral Migratory Stream (RMS) were not affected. In addition, these stimuli caused differential effects on the molecular components of the neurogenic niche; only prenatal LPS treatment reduced the local levels of TGF-&#946;1 mRNA in the DG. Finally, TGF-&#946;1 exerted its pro-neurogenic effects via the Smad2\/3 pathway in a neural stem cell culture.  Taken together, these data add evidence to the duration, regional specificity and dramatic consequences of prenatal immune programming on CNS physiology, compared with the limited response observed in the adult brain.","palabras_clave":"PRENATAL PROGRAMMING|INFLAMMATION|ADULT NEUROGENESIS|MICROGLIAL ACTIVATION|NEUROGENIC NICHE|TGF-BETA1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64186,"idioma_id":5,"titulo":"Rescate arqueológico de restos óseos humanos en el sitio Laguna Seca 1 (Pdo. de Gral. Lamadrid, Pcia. de Buenos Aires).","resumen":"RESUMEN. En el presente trabajo se comunican las tareas de rescate arqueológico de un entierro humano descubierto por pobladores locales en un predio ubicado a 15 kilómetros al norte de la ciudad de General Lamadrid, provincia de Buenos Aires. Los restos óseos se encontraban en el borde desmoronado de una pequeña barranca ubicada al costado de un camino que cortaba una lomada. Los objetivos planteados fueron: conocer la integridad del esqueleto, inferir la modalidad de entierro, estimar la cronología del evento inhumatorio y evaluar cuáles fueron los principales procesos postdepositacionales que afectaron a los elementos óseos. La compleja imbricación de los elementos óseos y el estado de fragmentación que presentaban, llevó a extraer el enterratorio en forma de bloque. Las tareas de excavación en el laboratorio permitieron recuperar un total de 121 especímenes óseos correspondientes a un único individuo adulto de sexo femenino datado en 579 ± 42 años AP. El registro de algunos elementos óseos articulados sugiere que se trata de un enterratorio primario que sufrió importantes modificaciones luego del evento de inhumación. Las alteraciones postdepositacionales, como el desplazamiento de las piezas óseas, la fragmentación de los huesos y la pérdida de varias unidades anatómicas, han estado vinculadas, en gran parte, a procesos naturales y antrópicos modernos.","palabras_clave":"rescate arqueológico|entierro humano|Holoceno tardío final|área Interserrana bonaerense"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64187,"idioma_id":4,"titulo":"Preliminary characterization of a bacteriocin-like substance produced by a Bacillus subtilis isolated from Argentinean vegetable food","resumen":"Bacillus spp. produces a large number of antimicrobial peptides and includes a variety of species of industrial importance. The aim of this study was to characterize the antimicrobial activity associated with a Bacillus subtilis strain. The activity of the crude bacteriocin-like substance (CBLS) was dependent on the components of the culture media and was detected in the exponential phase of growth. The CBLS was stable at a variable pH range and at 100 °C, but it was inactivated by sterilization conditions. It was also stable to storage at refrigeration and freezing temperatures for a considerable time. The mode of action of the antimicrobial activity was bacteriostatic and the effect on the cell membrane was determinated by Fourier transform infrared (FTIR) spectroscopy.","palabras_clave":"ANTIMICROBIAL PEPTIDES|BACILLUS CEREUS|BACILLUS SUBTILIS|BACTERIOCINS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64188,"idioma_id":4,"titulo":"The Evolution of the Cenozoic Terrestrial Mammalian Predator Guild in South America: Competition or Replacement?","resumen":"South America was isolated from other continents during most of the Cenozoic, developing a singular mammalian fauna. In contrast to North America, Europe, Asia, and Africa, up to the late Neogene, the carnivore adaptive zone in South America was populated by crocodiles (Sebecidae), large snakes (Madtsoiidae), large birds (Phorusrhacidae), and metatherian mammals (Sparassodonta). Sparassodonta were varied and comprised a wide range of body masses (&#8776; 2?50 kg) and food habits. Their diversity decreased towards the late Miocene (Huayquerian Stage\/Age) and the group became extinct in the ?middle? Pliocene (&#8776; 3 Ma, Chapadmalalan Stage\/Age). Several authors have suggested that the cause of this decline and extinction was the ingression of carnivorans to South America (about 6?7 Ma ago), because they competed with the Sparassodonta; although this hypothesis has been criticized in recent years. With the intention of testing the hypothesis of ?competitive displacement,? we review the fossil record of South American Sparassodonta and Carnivora, collect data about diversity, estimate size and diet, and determine first and last appearances. The diversity of Sparassodonta is low relative to that of Carnivora throughout the Cenozoic with the early Miocene (Santacrucian Stage\/Age) showing the greatest diversity with 11 species. In the late Miocene-middle Pliocene (Huayquerian Stage\/Age), the fossil record shows overlap of groups, and the Sparassodonta?s richness curve begins to decline with the first record of Carnivora. Despite this overlap, carnivorans diversity ranged from four or fewer species in the late Miocene-Pliocene to a peak of around 20 species in the early Pleistocene (Ensenadan Stage\/Age). Carnivora was initially represented by small-sized, omnivorous species, with large omnivores first appearing in the Chapadmalalan Stage\/Age. Over this period, Sparassodonta was represented by large and small hypercarnivores and a single large omnivorous species. From this review of the fossil record, it is suggested that factors other than competitive displacement may have caused the extinction of the Sparassodonta.","palabras_clave":"Sparassodonta|Carnivora|competitive displacement|ecological replacement"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64189,"idioma_id":5,"titulo":"Design of a Portable System for Continuous  Recording of Arterial Parameters during 24h","resumen":"The simultaneous measurement of pressure and diameter in a central artery during 24 hours could bring new information about the variability of the arterial mechanical properties. In order to perform these measurements in an animal, it is essential to count with a device that could acquire the signals from the sensors with a proper preconditioning circuit and then transfer them wirelessly to be stored and processed remotely. In this paper we present the prototype of a new portable and autonomous device, capable of being hosted on the animal's back. Preliminary results obtained from a pilot experience in a sheep using the prototype are detailed and limitations are discussed.","palabras_clave":"arterial stiffness|24-hour elasticity variability|ARM Cortex|conscious animal instrumentation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64190,"idioma_id":4,"titulo":"Histologic Changes After Urethroplasty Using Small Intestinal Submucosa Unseeded With Cells in Rabbits With Injured Urethra","resumen":"OBJECTIVE To determine whether small intestine submucosa has the same regenerative capacity when urethroplasty is performed in injured urethras.  METHODS Our experiment was conducted in 30 New Zealand male rabbits, all of which had urethral injury. One month after the injury, the animals were randomized into a control group or a group with onlay urethroplasty with small intestine submucosa. The animals were euthanized at 2, 4, 12, 24, and 36 weeks after urethroplasty, and their urethras were removed for histologic and immuno- histochemical examination. Before the scheduled euthanasia, urethrography and cystoscopy were performed.  RESULTS After 2 weeks, there was evidence of a continuous monolayer of stratified epithelial cells and absence of smooth muscle fibers. One month later, the epithelium showed no changes from the previously observed features, but some smooth muscle fibers (representing newly formed vessels) became apparent. After 3 months, the graft showed increased concentration of smooth muscle fibers. After 6 and 9 months, the density of smooth muscle cells remained unchanged. Fiber arrangement was irregular, particularly at the anastomosis site. Epithelial and smooth muscle phenotypes were confirmed by immunohistochemistry using anti-pan-citokeratin (AE1\/AE3) antibodies and anti-a-smooth muscle actin, respectively.  CONCLUSION Small intestine submucosa promotes regeneration in traumatized urethras, with slightly delayed epithelialization and abnormal distribution of smooth muscle. Urethral damage caused by trauma interferes with the normal healing process.","palabras_clave":"Uretra|SIS|Regeneraión|Medicina Regenerativa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64191,"idioma_id":4,"titulo":"Mo-based Keggin heteropolyacids as catalysts in the green and selective oxidation of diphenyl sul&#64257;de","resumen":"Heteropolyacids are used as redox catalysts due to their bifunctional properties. Some advantages over conventional catalysts, such as selectivity, easy isolation and reuse, are worth noting. In homogeneous conditions their isolation and recycling can be harder, so thatthey are supported for their use as heterogeneous catalysts.  In this work, we compare the catalytic activity of different heteropolyacids with Keggin structure, supported on pure and functionalized silica (with 3-aminopropyltrimethoxysilane). H6PMo11BO40, H6PMo11BiO40,H6PMo11LaO40,andH6PMo11YO40 weresynthesized, supportedandcharacterizedbyseveral physicochemical techniques. Highly selective results were obtained in the selective oxidation of  diphenyl sul&#64257;de to diphenyl sulfoxide or diphenyl sulfone. Besides, these catalysts can be reused. The  above-mentioned reaction is useful due to the importance of these compounds as intermediates in the  synthesis of biologically signi&#64257;cant molecules","palabras_clave":"GREEN SELECTIVE OXIDATION|DIPHENYL SULFIDE|SUPPORTED HETEROPOLYACID|KEGGIN HETEROPOLYACID"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64192,"idioma_id":4,"titulo":"Optimizing thymic recovery in HIV patients through multidrug therapies","resumen":"An optimal control approach based on an enlarged nonlinear model for the   dynamics of HIV infection and thymic function is composed to simulate   and evaluate antiretroviral therapies. In addition to the relevant   biological agents, an extra state variable is included, associated with   the thymus capacity for healthy cells production. The methodology   contemplates eventual deleterious effects of drugs over children´s   thymus recovery. The intake of \u2018Reverse Transcriptase Inhibitors\u2019 and   \u2018Protease Inhibitors\u2019 are modeled as two independent control variables,   each affecting a different term in the dynamics, so extending the   prevailing pure-HAART-therapy analysis. The objective function designed   here is also more inclusive than usual, accounting for the costs of the   two drug families involved and for the thymus deterioration, in addition   to penalizing eventual virus excess and healthy cells deficits. The   search for the best combined therapy is treated as an optimal control   problem. A hybrid version of Dynamic Programming for continuous and   discrete variables is used to treat the problem numerically. Long   time-horizons are explored, aiming to avoid typical peaks in drug   prescriptions found at the beginning and at the end of the optimization   periods. Results indicate that certain combinations of drugs are more   convenient than pure protocols when the value of thymus functioning is   relevant, specially for children patients.","palabras_clave":"HIV|Antiretroviral drugs|Optimal control|Nonlinear dynamics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64193,"idioma_id":4,"titulo":"Physicochemical properties and photodynamic activity of novel derivatives of Triarlylmethane and Thiazine","resumen":"Triarylmethane and thiazine dyes have attracted attention as anticancer and antimicrobial agents, due to  their structural features and selective localizations. Although these dyes have been initially explored in the  context of photodynamic therapy, some of these such as New Fuchsin and Azure B have still not been  extensively investigated. For this reason, we evaluated the chemical stability, aggregation effect, and  lipophilicity, as well as the photodynamic activity against LM-2 murine mammary carcinoma cells of five  new brominated dyes of triarylmethane and thiazine. These cationic compounds were obtained at high  purities and unequivocally characterized by conventional techniques. The introduction of bromine atoms  into the chromophoric system of New Fuchsin and Azure B dyes gave rise to a moderate bathochromic  shift and increased the lipophilicity, thereby improving their photophysical and photochemical properties  for biomedical applications.Moreover, the in vitro photodynamic activity demonstrated that, as the degree  of bromination increased, the phototoxicity remained unchanged or decreased. The lower efficiency to  inactivate cultured tumor cells may be attributed to the formation of the colorless carbinol pseudobase  and aggregation effects for triarylmethane and thiazine dyes, respectively. A promising strategy to reverse  the biological activity decrease observed might be the design of third-generation photosensitizers.","palabras_clave":"photodynamic activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64194,"idioma_id":4,"titulo":"A Chimeric UDP-glucose Pyrophosphorylase Produced by Protein Engineering Exhibits Sensitivity to Allosteric Regulators","resumen":"*Equally contributed to the workIn bacteria, glycogen or oligosaccharide accumulation involves    glucose-1-phosphate partitioning into either ADP-glucose (ADP-Glc) or   UDP-Glc. Their respective synthesis is catalyzed by allosterically   regulated ADP-Glc pyrophosphorylase (EC 2.7.7.27, ADP-Glc PPase) or   unregulated UDP-Glc PPase (EC 2.7.7.9). In this work, we characterized   the UDP-Glc PPase from Streptococcus mutans. In addition, we constructed a chimeric protein by cutting the C-terminal domain of the ADP-Glc PPase from Escherichia coli and pasting it to the entire S. mutans   UDP-Glc PPase. Both proteins were fully active as UDP-Glc PPases and   their kinetic parameters were measured. The chimeric enzyme had a   slightly higher affinity for substrates than the native S. mutans   UDP-Glc PPase, but the maximal activity was four times lower.   Interestingly, the  chimeric protein was sensitive to regulation by   pyruvate, 3-phosphoglyceric acid and  fructose-1,6-bis-phosphate, which   are known to be effectors of ADP-Glc PPases from different sources. The   three compounds activated the chimeric enzyme up to three-fold, and   increased the affinity for substrates. This chimeric protein is the   first reported  UDP-Glc PPase with allosteric regulatory properties. In   addition, this is a pioneer work dealing with a chimeric enzyme   constructed as a hybrid of two pyrophosphorylases with different   specificity toward nucleoside-diphospho-glucose and our results turn to   be relevant for a deeper understanding of the evolution of allosterism   in this family of enzymes.","palabras_clave":"protein engineering|allosteric regulation|pyrophosphorylases evolution|UDP-glucose|ADP-glucose"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64195,"idioma_id":4,"titulo":"Recombinant Monovalent Llama-Derived Antibody Fragments (VHH) to rotavirus VP6 Protect Neonatal Gnotobiotic Piglets Against Human Rotavirus Induced Diarrhea.","resumen":"Group A Rotavirus (RVA) is the leading cause of severe diarrhea in children. The aims of the present study were to determine the neutralizing activity of VP6-specific llama-derived single domain nanoantibodies (VHH nanoAbs) against different RVA strains in vitro and to evaluate the ability of G6P[1] VP6-specific llama-derived single domain nanoantibodies (VHH) to protect against human rotavirus in gnotobiotic (Gn) piglets experimentally inoculated with virulent Wa G1P[8] rotavirus. Supplementation of the daily milk diet with 3B2 VHH clone produced using a baculovirus vector expression system (final ELISA antibody -Ab- titer of 4096; virus neutralization -VN- titer of 256) for 9 days conferred full protection against rotavirus associated diarrhea and significantly reduced virus shedding. The administration of comparable levels of porcine IgG Abs only protected 4 out of 6 of the animals from human RVA diarrhea but significantly reduced virus shedding. In contrast, G6P[1]-VP6 rotavirus-specific IgY Abs purified from eggs of hyperimmunized hens failed to protect piglets against human RVA-induced diarrhea or virus shedding when administering similar quantities of Abs. The oral administration of VHH nanoAb neither interfered with the host´s isotype profiles of the Ab secreting cell responses to rotavirus, nor induced detectable host Ab responses to the treatment in serum or intestinal contents. This study shows that the oral administration of rotavirus VP6-VHH nanoAb is a broadly reactive and effective treatment against rotavirus-induced diarrhea in neonatal pigs. Our findings highlight the potential value of a broad neutralizing VP6-specific VHH nanoAb as a treatment that can complement or be used as an alternative to the current strain-specific RVA vaccines. Nanobodies could also be scaled-up to develop pediatric medication or functional food like infant milk formulas that might help treat RVA diarrhea.","palabras_clave":"VHH|rotavirus|recombinant monoclonal nanantibody|diarrhea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64196,"idioma_id":4,"titulo":"Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)","resumen":"The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA\/Vicuña-wt\/ARG\/C75\/2010\/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA\/Guanaco-wt\/ARG\/Chubut\/1999\/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation.","palabras_clave":"vicugna|south american camelid|rotavirus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64197,"idioma_id":4,"titulo":"Equine G3P[3] rotavirus strain E3198 related to simian RRV and feline\/canine-like rotaviruses based on complete genome analyses","resumen":"Equine group A rotavirus (RVA) strains are the most important cause of gastroenteritis in equine neonates and foals worldwide, and G3P[12] and G14P[12] are epidemiologically the most important genotypes. The genotype constellation of an unusual Argentinean G3P[3] RVA strain (RVA\/Horse-wt\/E3198\/2008\/G3P[3]) detected in fecal samples of a diarrheic foal in 2008 was shown to be G3-P[3]-I3-R3-C3-M3-A9-N3-T3-E3-H6. Each of these genotypes has been found typically in feline and canine RVA strains, and the genotype constellation is reminiscent to those of Cat97-like RVA strains. However, the phylogenetic analyses revealed only a distant relationship between E3198 and known feline, canine and feline\/canine-like human RVA strains. Surprisingly, a rather close relationship was found between E3198 and simian RVA strains RVA\/Simian-tc\/USA\/RRV\/1975\/G3P[3] for at least 5 gene segments. RRV is believed to be a reassortant between a bovine-like RVA strain and a RVA strains distantly related to feline\/canine RVA strains. These analyses indicate that E3198 is unlikely to be of equine origin, and most likely represents a RVA interspecies transmitted virus, possibly in combination with one or more reassortments, from a feline, canine or related host species to a horse. Further studies are in progress to evaluate if this strain was a single interspecies transmission event, or if this strain started to circulate in the equine population.","palabras_clave":"rotavirus|potrillo|diarrea|typing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64198,"idioma_id":4,"titulo":"TGF-beta specifically enhances the metastatic attributes of murine lung adenocarcinoma: implications for human non-small cell lung cancer","resumen":"Lung cancer is the most frequent and one of the most deadly cancer types and is classified into small cell lung cancer and non-small cell lung cancer (NSCLC). Transforming growth factor beta (TGF&#946;) regulates a wide array of cell functions and plays a major role in lung diseases, including NSCLC. TGF&#946; signals through the complex of TGF&#946; type I and type II receptors, triggering Smad and non-Smad signaling pathways such as PI3K\/Akt and MEK1\/ERK. We investigated the role of TGF&#946;1 on the progression of the murine lung adenocarcinoma cell line LP07. Furthermore, we undertook a retrospective study with tissue samples from stage I and II NSCLC patients to assess the clinical pathologic role and prognostic significance of T&#946;RI expression. We demonstrated that although lung cancer cell monolayers responded to TGF&#946;1 anti-mitogenic effects and TGF&#946;1 pulse (24 h treatment) delayed tumor growth at primary site; a switch towards malignant progression upon TGF&#946;1 treatment was observed at the metastatic site. In our model, TGF&#946;1 modulated in vitro clonogenicity, protected against stress-induced apoptosis and increased adhesion, spreading, lung retention and metastatic outgrowth. PI3K and MEK1 signaling pathways were involved in TGF&#946;1-mediated metastasis stimulation. Several of these TGF&#946; responses were also observed in human NSCLC cell lines. In addition, we found that a higher expression of T&#946;RI in human lung tumors is associated with poor patient´s overall survival by univariate analysis, while multivariate analysis did not reach statistical significance. Although additional detailed analysis of the endogenous signaling in vivo and in vitro is needed, these studies may provide novel molecular targets for the treatment of lung cancer.","palabras_clave":"NON SMALL CELL LUNG CANCER|METASTASIS|TGF BETA|SIGNALING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64199,"idioma_id":5,"titulo":"Uso de Nuevas Tecnologias de la comunicacion en un curso introductorio de quimica para alumnos ingresantes de ingenieria","resumen":"Con el objetivo de acompañar a los alumnos ingresantes a la Facultad de Ingeniería en sus primeras experiencias de estudio en la universidad, se ofreció un curso virtual introductorio de Química (de carácter optativo) denominado Nomenclatura de Compuestos Inorgánicos. Se realizó a través de la plataforma educativa Moodle desde el campus virtual de la UNPSJB.    Si bien el número de alumnos inscriptos (61) fue importante, pocos realizaron todas las actividades propuestas. Solamente un 34% enviaron la primera de las tareas propuestas para la revisión del tutor. No obstante, el 50% de ellos completó el curso, con muy buenos resultados, tanto durante el desarrollo del curso virtual como durante la cursada de Química de primer año.  El aula virtual constituye una herramienta válida para favorecer la articulación con el nivel educativo previo y la integración de los estudiantes a la vida universitaria.","palabras_clave":"AULA VIRTUAL|NOMENCLATURA DE COMPUESTOS INORGANICOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64200,"idioma_id":4,"titulo":"Novel heterozygous mutation in the steroidogenic acute regulatory protein gene in a 46,XY patient with congenital lipoid adrenal hyperplasia","resumen":"StAR facilitates cholesterol entry into the mitochondria as part of the transduceosome complex. Recessive mutations in the gen STAR cause classic and nonclassic congenital lipoid adrenal hyperplasia. The aim of the study was to analyze the molecular consequences of a novel heterozygous STAR mutation in a 46,XY patient with ambiguous genitalia and adrenal insufficiency. We found a de novo heterozygous IVS-2A&gt;G STAR mutation and the reported heterozygous p.G146A SF1 polymorphism with normal CYP11A1, FDXR, FDX1, VDAC1 and TSPO genes. RT-PCR and sequencing from patient´s testicular RNA showed a -exon2 transcript and the wild-type (WT) transcript. Both 37 kDa precursor and 30 kDa mature protein were detected in COS-7 cell transfected with mutant and WT plasmids. Immunofluorescence showed almost no co-localization of mitochondria and mutant protein (delta22-59StAR). Delta22-59StAR activity was 65±13% of WT. Cotransfection with WT and delta22-59StAR plasmids reduced WT activity by 62.0% ± 13.9. Novel splice-junction heterozygous STAR mutation (IVS-2A&gt;G) resulted in the in-frame loss of amino acids 22 to 59 in the N-terminal mitochondrial targeting signal. A misfolded p.G22_L59delStAR might interfere with WT StAR activity by blocking the transduceosome complex, causing an autosomal dominant form of StAR deficiency, explaining the clinical phenotype.","palabras_clave":"STaR|Mutation|Acute regulatory protein"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64201,"idioma_id":4,"titulo":"Presence of Gumprecht shadows (smudge cells) in bovine leukemia virus-positive cattle","resumen":"Enzootic Bovine Leukosis is a chronic disease caused by the bovine leukemia virus (BLV). Smudge cells, also known as Gumprecht shadows, are not simple artifacts of slide preparation, but ragged lymphoid cells found mainly in peripheral blood smears from human patients with chronic lymphocytic leukemia. In this study, we report the presence of Gumprecht shadows in peripheral blood from BLV-positive cattle.","palabras_clave":"Bovine Leukemia|GUMPRECHT SHADOWS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64202,"idioma_id":5,"titulo":"Efecto de las fitohormonas sobre el control de la fructificación en manzanos","resumen":"Con el objetivo de evaluar el efecto de diferentes fitohormonas en  el raleo de frutos en manzanos se realizaron dos experimentos en  la cv. Royal Gala. En uno, la cultivar estaba injertada sobre portainjerto  M9 y en el otro sobre M4. Los tratamientos aplicados en  ambos ensayos fueron: 1: Benciladenina (BA) 100 ppm cuando la  media de diámetro de fruto (DF) era de 8 a 12 mm; 2: Acido Naftalen  Acético (ANA) 10 ppm a 5 mm DF; 3: ANA 10 ppm a 5 mm  DF + BA 50 ppm con DF de 8 a 12 mm; 4: ANA10 ppm a 5 mm  DF + BA 75 ppm con DF de 8 a 12 mm; 5: ANA 10 ppm a 5 mm  FD + BA 100 ppm con DF de 8 a 12 mm; 6: Testigo: raleo manual.  Las variables de respuesta fueron: número de frutos cuajados por  área de la sección transversal de la rama (ASR); número de frutos  cuajados cada 100 ramilletes florales; distribución del cuaje (porcentaje  de dardos donde cuajaron 0, 1 o más de 1 fruto). En cosecha  se evaluó el peso, diámetro mayor, diámetro menor y longitud  de cada fruto y se realizó una clasificación de tamaños comerciales  de acuerdo a una caja Mark IV de 18 kg. Los datos se analizaron  mediante el análisis de la Varianza (ANOVA); prueba Chi cuadrado  y análisis de componentes principales. Todas las combinaciones  ANA + BA fueron efectivas en el raleo de frutos y resultaron  en un cuaje y tamaño de frutos similares. Por lo tanto aplicaciones  de ANA 10 ppm a 5 mm DF + BA 50 ppm son una buena  alternativa para el control de la fructificación en Royal Gala. Los  tamaños comerciales de las manzanas tratadas con BA fueron superiores  a los del testigo.","palabras_clave":"Malus domestica| 6-Benciladenina|Acido Naftalen Acético,|raleo de frutos,"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64203,"idioma_id":5,"titulo":"Regulación de la carga frutal en agroecosistemas de perales mediante programas de extinción de frutos","resumen":"El objetivo del presente trabajo fue regular la carga frutal mediante  extinción de frutos en agroecosistemas de hoja caduca. Para ello,  en dos agroecosistemas de perales cv. Williams se realizaron los  siguientes tratamientos: 1: 6-Benciladenina (BA): 200 ppm cuando  la media de diámetro del fruto (DF) era de 20 mm; 2: BA 150 ppm  a 14 mm DF; 3: BA 75 ppm a 7 mm DF + otra aplicación de BA  75 ppm a 14 mm DF; 4: Urea 7,5 % a 80 % de floración; 5: Urea  7,5 % a 80 % de floración + una aplicación de BA 75 ppm a 14  mm DF; 6: Testigo: raleo manual. Las variables analizadas fueron:  número de frutos cuajados\/Área Seccional de Rama (ASR); número  de frutos cuajados·100 ramilletes florales-1; distribución del  cuaje: porcentaje de dardos donde cuajaron 0, 1 o más de 1 fruto  y número de frutos raleados manualmente·cm-2 ASR. A cosecha  se evaluaron los tamaños comerciales de los frutos en una caja estándar  de 18,2 kg. Los datos se analizaron utilizando el procedimiento  de Análisis de la Varianza; prueba Chi cuadrado y análisis  de componentes principales. En los árboles tratados con BA se observó  una mayor extinción de frutos al utilizar concentraciones de  150 ppm a 14 mm DF o aplicaciones repetidas de 75 ppm a 7 y 14  mm DF. Concentraciones de BA de 200 ppm aplicadas a 20 mm  DF no fueron efectivas ya que su aplicación fue muy tardía. Los  tratamientos con urea no lograron regular la carga frutal en forma  significativa. En los tratamientos con BA se observó un mayor  porcentaje de frutos de tamaños grandes. En uno de los agroecosistemas,  las aplicaciones repetidas de BA a 75 ppm mostraron el  mayor porcentaje de frutos grandes (110, 120 y 135), estos tres tamaños  juntos representaron el 58,12 % de los frutos cosechados  de ese tratamiento y en el otro agroecosistema, el tratamiento combinado  de Urea + BA 75 ppm fue el que obtuvo el mayor porcentaje  de estos tres tamaños.","palabras_clave":"Pyrus communis|RALEO DE FRUTOS|BENCILADENINA UREA|TAMAÑO DEL FRUTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64204,"idioma_id":4,"titulo":"Cellular remodelling by apoptosis during porcine placentation","resumen":"The mechanisms that regulate the apoptosis are essential to the normal development and maintenance of homoeostasis and play an important role in placental development in mammals. During porcine pregnancy, there must be a proper cellular remodelling to achieve a normal gestational development. Knowledge of pig physiology during pregnancy will explore options to increase the productivity of this species of high economical value. The purpose of this work was to study the cell morphology and apoptosis of porcine placentas from early, mid and late pregnancy. For that purpose, high-resolution light microscopy and transmission electron microscopy were performed to the study of cell morphology. TUNEL, the apoptosis index (IAp) and the expression of c-FLIP through immunohistochemistry technique were used to the study of apoptosis. High-resolution light microscopy and transmission electron microscopy confirmed the presence of placental cells with ultrastructural apoptotic features. Apoptotic nuclei were detected by TUNEL in different placental structures and phagocytes containing apoptotic bodies. The IAp in villi was 9.34% at early, 0.82% at mid and 23.85% at late pregnancy. Statistically significant differences were found between periods (p < 0.05). In previous studies, we determined a differential induction of the apoptotic routes in the placental villi in agreement with the gestational period. A co-expression of receptors and mitochondrial proteins in placental connective tissue was detected, but the immunolocalization of c-FLIP would indicate an endogenous inhibition of the extrinsic pathway. In conclusion, in swine there exists differential activation of inducing apoptotic pathways in different placental structures according to the gestational period.","palabras_clave":"Apoptosis|Porcine|Placentation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64205,"idioma_id":4,"titulo":"Strategies to Achieve Sustainability and Quality in Birth Defects Registries: The Experience of the National Registry of Congenital Anomalies of Argentina","resumen":"In many low- and middle-income countries, birth defects are not considered a public health priority and are perceived  by the medical community as rare, unpreventable events. In this context, a registry of birth defects should address  not only the collection, analysis, and dissemination of information but also contribute to local interventions like prevention,  diagnosis, and treatment. We describe the National Registry of Congenital Anomalies of Argentina (RENAC) in terms  of case definition, data collection, quality assurance, and data sending, coding, analysis, and information dissemination  and we present the strategies used to ensure its sustainability. We emphasize strategies for motivating the people collecting  data, such as training activities, participation in research projects, returning the processed data, making useful clinical  information available, giving non-monetary rewards, and linking cases to genetic services.","palabras_clave":"BIRTH DEFECTS|PUBLIC HEALTH SURVEILLANCE|REGISTRY |INFANT MORTALITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64206,"idioma_id":4,"titulo":"Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification","resumen":"Bovine lactoferricin (Lfcin B) belongs to the antimicrobial peptide family, which is the first line of defense against pathogens in many organisms. Lfcin B has important applications due to its antiviral, antifungal, antiparasitic, anticancer\/tumor and antibacterial activity.    In this work, we tested five triazine dyes for Lfcin B affinity interactions using surface plasmon resonance (SPR) technology. Recombinant Lfcin B was expressed as a fusion protein with GST (Lfcin B-GST) by using the baculovirus expression vector system and the dye-Sepharose matrices were assayed for Lfcin B-GST adsorption and subsequent elution.    Red HE-3B and Yellow HE-4R dyes were selected and immobilized on a Sepharose-4B matrix for further purification studies. The Yellow HE-4R-Sepharose matrix was specific for Lfcin B and allowed adsorption of Lfcin B-GST directly from the culture medium even at high salt concentration.    This novel application of SPR to screen possible dye?peptide interactions could be relevant to purify other peptides or proteins by using low-cost dye-affinity chromatography.","palabras_clave":"TRIAZINIC DYE|SURFACE PLASMON RESONANCE|BOVINE LACTOFERRICIN|PURIFICATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64207,"idioma_id":5,"titulo":"Deficits sociales en adolescentes con sobrepeso y obesidad","resumen":"El  objetivo de este trabajo fue identificar las habilidades sociales de  adolescentes con sobrepeso y obesidad de contexto urbano y rural de Tucumán. Se  realizaron mediciones antropométricas a una muestra de 276 adolescentes asistentes  a escuelas públicas, entre 11 y 20 años, administrándoles además la Batería de  Socialización (BAS-3) y una encuesta nutricional como sociodemográfica. Se  encontró un 18% de adolescentes con diagnostico de sobrepeso y obesidad. Este  grupo refería mayor retraimiento y ansiedad social al momento de iniciar sus  relaciones sociales respecto a sus pares normopeso. Se observó un mayor nivel  de ansiedad social y un menor autocontrol en los adolescentes obesos urbanos. Los  resultados encontrados destacan la comorbilidad entre estos trastornos de  alimentación e indicadores psicopatológicos.&nbsp;  Profundizar la investigación de las características de la socialización  en adolescentes con TCA, particularmente obesidad, permitirá diseñar  estrategias de intervención en población urbana y rural.","palabras_clave":"adolescentes|sobrepeso|obesidad|retraimiento social|ansiedad social"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64208,"idioma_id":4,"titulo":"Restructuring Fortran legacy applications for parallel computing in multiprocessors","resumen":"As it is widely known, multi-core computers are broadly used these days, and automatic parallelization of sequential programs is still a challenge. In this context, we propose a set of code transformations to be applied automatically by a tool in order to transform sequential legacy systems into their parallel version. We implement these transformations by applying a lightweight source code analysis based on rewritable AST (Abstract Syntax Tree). Since it is not always possible to automatically parallelize the code, we also implemented some specific analyses in order to report possible changes that would allow specific parallelization. Additionally, we present some examples in which these transformations were conducted and the corresponding performance experiments.","palabras_clave":"Hihg Performance Computing|Legacy Applications"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64209,"idioma_id":5,"titulo":"Globalización, ciencia y biotecnología","resumen":"En la actualidad ?controlar los procesos de reproducción de la vida y de la propia riqueza  es simultáneamente un fin y un mecanismo de construcción de poder? (Ceceña, 2001).  En este sentido, la biotecnología significó la condensación de la lógica del biopoder, a  través de la posibilidad de controlar los organismos vivos desde su génesis, imprimiendo  una lógica mercantil. A través de este control, la naturaleza es abordada bajo una  dinámica intrusiva de seguimiento, apropiación y manipulación. Con el fin de infiltrar  cada vez más la vida y administrarla, la instauración del biopoder, a partir de diversas  estrategias ?como las nuevas tecnologías-, impregna el cuerpo social y la conciencia  de los individuos con el fin de subsumir cada aspecto de la sociedad a la mirada del  capital. La biotecnología ha permitido interpretar, absorber, y rearticular las relaciones  humanas con el ambiente, a partir de las necesidades del mercado capitalista. Así la  naturaleza, en constante apropiación, se ve permanentemente amalgamada a los ritmos  productivos del capital.La necesidad deacumulacióny expansión del capital en la etapa  reciente ha alcanzado la producción y reproducción de la vida como nuevo escenario de  dominación definiendo, en términos de la lógica mercantil, las condiciones de existencia  de los organismos biológicos. Es en este sentido que podemos empezar a pensar en las  lógicas del mercado capitalista como incrustadas en los procesos de la vida. O bien,  visualizar esos procesos de la vida ahora insertos en el mercado capitalista. Es decir,  no sólo comenzamos a hablar de la expansión del control de los mercados capitalistas  sino también de la incorporación de cuestiones que antes no eran plausibles de ser  mercantilizadas y apropiadas, como la naturaleza o el conocimiento.Indizada en Ulrich´s&nbsp;, Latindex, Publindex","palabras_clave":"BIOTECNOLOGIA|CIENCIA|GLOBALIZACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64210,"idioma_id":4,"titulo":"Insights into Xanthomonas axonopodis pv. citri biofilm through proteomics","resumen":"Background: Xanthomonas axonopodis pv. citri (X. a. pv. citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and several lines of evidence suggest that in X. a. pv. citri this process is a requirement to achieve maximal virulence since it has a major role in host interactions. In this study, proteomics was used to gain further insights into the functions of biofilms. Results: In order to identify differentially expressed proteins, a comparative proteomic study using 2D difference gel electrophoresis was carried out on X. a. pv. citri mature biofilm and planktonic cells. The biofilm proteome showed major variations in the composition of outer membrane proteins and receptor or transport proteins. Among them, several porins and TonB-dependent receptor were differentially regulated in the biofilm compared to the planktonic cells, indicating that these proteins may serve in maintaining specific membrane-associated functions including signaling and cellular homeostasis. In biofilms, UDP-glucose dehydrogenase with a major role in exopolysaccharide production and the non-fimbrial adhesin YapH involved in adherence were over-expressed, while a polynucleotide phosphorylase that was demonstrated to negatively control biofilm formation in E. coli was down-regulated. In addition, several proteins involved in protein synthesis, folding and stabilization were up-regulated in biofilms. Interestingly, some proteins related to energy production, such as ATP-synthase were down-regulated in biofilms. Moreover, a number of enzymes of the tricarboxylic acid cycle were differentially expressed. In addition, X. a. pv. citri biofilms also showed down-regulation of several antioxidant enzymes. The respective gene expression patterns of several identified proteins in both X. a. pv. citri mature biofilm and planktonic cells were evaluated by quantitative real-time PCR and shown to consistently correlate with those deduced from the proteomic study.Conclusions: Differentially expressed proteins are enriched in functional categories. Firstly, proteins that are downregulated in X. a. pv. citri biofilms are enriched for the gene ontology (GO) terms ?generation of precursor metabolites and energy? and secondly, the biofilm proteome mainly changes in ?outer membrane and receptor or transport?. We argue that the differentially expressed proteins have a critical role in maintaining a functional external structure as well as enabling appropriate flow of nutrients and signals specific to the biofilm lifestyle.","palabras_clave":"CITRUS|CANKER|BIOFILM|PROTEOMICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64211,"idioma_id":4,"titulo":"NADPH oxidase derived reactive oxygen species are involved in human neutrophil IL-1&beta; secretion but not in inflammasome activation","resumen":"Neutrophils are essential players in acute inflammatory responses. Upon stimulation, neutrophils activate NADPH oxidase, generating an array of reactive oxygen species (ROS). Interleukin-1 beta (IL-1&#946;) is a major proinflammatory cytokine synthesized as a precursor that has to be proteolytically processed to become biologically active. The role of ROS in IL-1&#946; processing is still controversial and has not been previously studied in neutrophils. We report here that IL-1&#946; processing in human neutrophils is dependent on caspase-1 and on the serine proteases elastase and\/or proteinase 3. NADPH oxidase deficient neutrophils activated caspase-1 and did not exhibit differences in NALP3 expression, indicating that ROS are neither required for inflammasome activation nor for its priming, as has been reported for macrophages. Strikingly, ROS exerted opposite effects on the processing and secretion of IL-1&#946;; whereas ROS negatively controlled caspase-1 activity, as reported in mononuclear phagocytes, ROS were found to be necessary for the exportation of mature IL-1&#946; out of the cell, a role never previously described. The complex ROS-mediated regulation of neutrophil IL-1&#946; secretion might constitute a physiological mechanism to control IL-1&#946;-dependent inflammatory processes where neutrophils play a crucial role.","palabras_clave":"NEUTROPHILS|ROS|IL-1BETA|INFLAMMASOME"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64212,"idioma_id":4,"titulo":"Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster","resumen":"Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis  screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in  holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 coisogenic  single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by  strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the  candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster.","palabras_clave":"Drosophila|Morphological traits|Candidate genes|P-element mutagenesis|Temperature|Phenotypic plasticity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64213,"idioma_id":4,"titulo":"The evaluation of innovation processes from the perspective of territorial development","resumen":"The object of the present work is to investigate the degree of   adjustment of the ?evaluation studies and analysis? that are promoted   when validating investment processes which involve ?innovative? actions;   from the contrast between the practises and the instruments applied by   the main public actor of the regional innovation system with the   objectives that such policies and\/or programs hold as justification. In   this analysis, it is verified the ability with which the evaluation   practises held can generate readings of the potential impacts which are   to be known in advance both, by the present evaluation models and by the   unanswered questions which come out from these new technological risks   and uncertainties. From this analysis performed, it arises that the   promoted practises do not adjust completely neither to the traditional   evaluation theoretical standards nor to the emerging demands which   contemplate the new conceptualizations of risk; giving place to mistaken   evaluations in relation to the contributions of such social efforts to   the territorial development; an achievement which is considered the main   objective of them.","palabras_clave":"innovación|desarrollo |territorio"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64214,"idioma_id":4,"titulo":"Effect of concentration on the photophysics of dyes in light scattering materials","resumen":"Photoactive materials based on dye molecules  incorporated into thin films or bulk solids are useful for applications as photosensitization,  photocatalysis, solar cell sensitization and fluorescent labeling, among others.  In most cases, high concentrations of dyes are desirable to maximize light  absorption. Under these circumstances, the proximity of dye molecules leads to  the formation of aggregates and statistical traps, which dissipate the  excitation energy and lower the population of excited states. The search for  enhancement of light collection avoiding energy wasting requires accounting the  photophysical parameters quantitatively, including the determination of quantum  yields, complicated by the presence of light scattering when particulate  materials are considered. In the present work we summarize recent advances on  the photophysics of dyes in light scattering materials, with particular focus  on the effect of dye concentration. We show how experimental reflectance,  fluorescence and laser induced optoacoustic spectroscopy data can be used together  with theoretical models for the quantitative evaluation of inner filter  effects, fluorescence and triplet formation quantum yields and energy transfer  efficiencies.","palabras_clave":"DYE|CONCENTRATION|LIGHT SCATTERING|PHOTOPHYSICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64215,"idioma_id":4,"titulo":"Evidence on dye clustering in the sensitization of TiO2 by aluminum phthalocyanine","resumen":"As  previous studies have shown, the photocatalytical reduction of Cr(VI) to Cr(III)  in the presence of 4-chlorophenol can be carried out efficiently under visible  irradiation using TiO2 modified with hydroxoaluminum-tricarboxymonoamide  phthalocyanine (AlTCPc) in spite of the high aggregation tendency of the dye.  In the present work, photocurrent and absorption spectra of AlTCPc modified TiO2  films are studied together with absorption and fluorescence of the dye in  solution as a function of the concentration of the dye to clarify a) the role  of aggregates and the nature of the species responsible for electron injection  into the semiconductor and b) the reasons why, as reported earlier, the  photocatalytical activity is nearly independent on dye loading at constant TiO2  mass. Results are consistent with the presence of AlTCPc clusters with similar  properties both on the TiO2 surface and in H2O\/DMSO  solution. The actual photoactive species is the monomeric dye electronically  coupled to the semiconductor. Monomer concentration depends only slightly on AlTCPc  analytical concentration, in a way similar to surfactant monomers in micellar  equilibrium, thus explaining the independence of photocatalytic activity on dye  concentration.","palabras_clave":"DYE|SEMICONDUCTOR|SENSITIZATION|CLUSTER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64216,"idioma_id":5,"titulo":"Capitalismo y Estado. Reflexiones desde la Teoría de la dependencia en América Latina","resumen":"En el presente trabajo se sistematizará la llamada corriente de la dependencia o dependentismo, siempre en diálogo con el problema del desarrollo, haciendo eje en sus principales autores e indagando en la concepción de capitalismo, articulado en relación con la estructura de clases y el rol del Estado, para revisar sus principales propuestas académico-políticas. Paralelamente, pretendemos reflexionar acerca de los aportes de estos autores para comprender los procesos de mundialización capitalista. El capitalismo ha ido mutando en su necesidad de garantizar la acumulación. La apropiación del excedente se realiza hoy permeando las fronteras nacionales y por ello el imperialismo, el subdesarrollo y la dependencia se han profundizado. Esto nos lleva a pensar, como hicieron los representantes de esta teoría, más allá de los discursos que circulan desde gobiernos o desde los medios de comunicación, sobre la posibilidad\/imposibilidad de América Latina de alcanzar un desarrollo autónomo así como sobre la validez de las categorías analíticas de la Teoría de la dependencia.","palabras_clave":"TEORÍA DE LA DEPENDENCIA|CAPITALISMO|ESTADO|ESTRUCTURA DE CLASES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64217,"idioma_id":4,"titulo":"'Natural AChE inhibitors from plants and their contribution to Alzheimer?s disease therapy'","resumen":"As acetylcholinesterase (AChE) inhibitors are an important therapeutic strategy in Alzheimer?s disease, efforts are being made in search of new molecules with anti-AChE activity. The fact that naturally-occurring compounds from plants are considered to be a potential source of new inhibitors has led to the discovery of an important number of secondary metabolites and plant extracts with the ability of inhibiting the enzyme AChE, which, according to the cholinergic hypothesis, increases the levels of the neurotransmitter acetylcholine in the brain, thus improving cholinergic functions in patients with Alzheimer?s disease and alleviating the symptoms of this neurological disorder. This review summarizes a total of 128 studies which correspond to the most relevant research work published during 2006-2012 (1st semester) on plant-derived compounds, plant extracts and essential oils found to elicit AChE inhibition.","palabras_clave":"ALZHEIMER'S DISEASE|ACETYLCHOLINESTERASE INHIBITORS|SECONDARY METABOLITES|PLANT EXTRACTS|ESENTIAL OILS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64218,"idioma_id":4,"titulo":"Synthesis and acetylcholinesterase inhibitory activity of polyhydroxylated sulfated steroids:  structure\/activity studies","resumen":"Disulfated and trisulfated steroids have been synthesized from cholesterol and  their acetylcholinesterase inhibitory activity has been  evaluated. In our  studies we have found that the activity was not only  dependent on the location of the sulfate groups but on their configurations. 2b,3a,6a-trihydroxy-5a-cholestan-6-one  trisulfate (18)  was the most active steroid with an IC50 value of 15.48 mM comparable to  that of 2b,3a-dihydroxy-5a-cholestan-6-one  disulfate (1). Both compounds were found to be less active than the reference  compound eserine. The butyrylcholinesterase activity of 1 and 18 was one  magnitude lower than that against acetylcholinesterase revealing a selective  inhibitor profile.","palabras_clave":"SULFATED STEROIDS|SYNTHESIS|ACETYLCHOLINESTERASE ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64219,"idioma_id":5,"titulo":"Extracción  y caracterización físico-química del aceite de semilla de ?chañar? (Geoffroea decorticans) (fabaceae) de la región central de la provincia de Formosa  (Argentina)","resumen":"El objetivo del presente trabajo fue evaluar las características físico-químicas  del aceite contenido en las semillas de los frutos de chañar. Además, se evaluaron el rendimiento y las condiciones de extracción por prensado en frío.  Se trabajó con dos clases de harina: harina de semillas obtenida manualmente (HSM) y harina de semillas obtenidas por método mecánico, la cual contiene fracciones de endocarpio leñoso (HSL). El aceite extraído fue de un 50 % para la HSM y 22 % para la HSL, todos estos valores expresados en base seca. El prensado de HSL llevado a cabo mediante el uso de  una prensa a tornillo, produjo un rendimiento del 10 % de aceite crudo. Tanto para el aceite obtenido a partir de HSM como el del obtenido a partir de HSL se realizaron las siguientes determinaciones analíticas: índice de acidez (2,49; 2,43), índice de saponificación (164), índice de refracción a 25 °C (1,472; 1,468), índice de iodo (114; 114,5) y densidad (0,8979; 0,8788). El perfil de ácidos grasos, muestra la presencia de ácidos grasos insaturados, principalmente ácido oleico (35,9%) y ácido linoleico (41,6%) con una relación oleico\/linoleico de 0,86; mientras que la relación insaturados\/saturados fue de 3,5. El análisis de resonancia magnética nuclear no indica presencia de ácidos ciclopropenoides (estercúlico y malválico), considerados nocivos para el organismo, para el límite de detección del método (< 1%).","palabras_clave":"GEOFFROEA DECORTICANS|SEMILLA|ÁCIDOS GRASOS|RESONANCIA MAGNÉTICA NUCLEAR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64220,"idioma_id":4,"titulo":"Estrogen receptor beta growth inhibitory effects are repressed through activation of MAPK and PI3K signalling in mammary epithelial and breast cancer cells.","resumen":"Two thirds of breast cancers express estrogen receptors (ER). ER alpha (ER&#945;) mediates breast cancer cell proliferation, and expression of ER&#945; is the standard choice to indicate adjuvant endocrine therapy. ERbeta (ER&#946;) inhibits growth in vitro; its effects in vivo have been incompletely investigated and its role in breast cancer and potential as alternative target in endocrine therapy needs further study. In this work, mammary epithelial (EpH4 and HC11) and breast cancer (MC4-L2) cells with endogenous ER&#945; and ER&#946; expression and T47-D human breast cancer cells with recombinant ER&#946; (T47-DER&#946;) were used to explore effects exerted in vitro and in vivo by the ER&#946; agonists 2,3-bis (4-hydroxy-phenyl)-propionitrile (DPN) and 7-bromo-2-(4-hydroxyphenyl)-1,3-benzoxazol-5-ol (WAY). In vivo, ER&#946; agonists induced mammary gland hyperplasia and MC4-L2 tumour growth to a similar extent as the ER&#945; agonist 4,4´,4´´-(4-propyl-(1H)-pyrazole-1,3,5-triyl) trisphenol (PPT) or 17&#946;-estradiol (E2) and correlated with higher number of mitotic and lower number of apoptotic features. In vitro, in MC4-L2, EpH4 or HC11 cells incubated under basal conditions, ER&#946; agonists induced apoptosis measured as upregulation of p53 and apoptosis-inducible factor protein levels and increased caspase 3 activity, whereas PPT and E2 stimulated proliferation. However, when extracellular signal-regulated kinase 1 and 2 (ERK ½) were activated by co-incubation with basement membrane extract or epidermal growth factor, induction of apoptosis by ER&#946; agonists was repressed and DPN induced proliferation in a similar way as E2 or PPT. In a context of active ERK ½, phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)\/RAC-alpha serine\/threonine-protein kinase (AKT) signalling was necessary to allow proliferation stimulated by ER agonists. Inhibition of MEK ½ with UO126 completely restored ER&#946; growth-inhibitory effects, whereas inhibition of PI3K by LY294002 inhibited ER&#946;-induced proliferation. These results show that the cellular context modulates ER&#946; growth-inhibitory effects and should be taken into consideration upon assessment of ER&#946; as target for endocrine treatment.","palabras_clave":"estrogen receptors|estrogen receptor beta antagonists|breast cancer"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64221,"idioma_id":4,"titulo":"Novel, low cost, highly effective, handmade steroid pellets for experimental studies","resumen":"The basic component of SilasticH glue (Dow Corning) used to prepare SilasticH pellets is polydimethylsiloxane. This  compound is also present in other commercial adhesives such as FASTIXH (Akapol SA) that are available in any store for that  category. In the present study we developed low cost, easy to prepare handmade steroid pellets (HMSP) by mixing 17bestradiol,  progesterone or other synthetic steroids with FASTIXH adhesive. We assessed serum levels of 17b-estradiol,  progesterone, prolactin and luteinizing hormone in ovariectomized mice treated for 24 and 48 h or 7, 14 and 28 days with  20 mg or 5 mg of 17b-estradiol or 5 mg progesterone HMSP. We found a time dependent and significant increase in the  levels of both natural hormones, and a downregulation of serum luteinizing hormone levels, while both 17b-estradiol doses  increased serum prolactin. Uterine weights at sacrifice and histological examination of the uteri and the mammary glands  correlated with estrogen or progestin action. Finally, we evaluated the biological effects of HMSP compared to commercial  pellets or daily injections in the stimulation or inhibition of hormone dependent mammary tumor growth, and found that  HMSP were as effective as the other methods of hormone administration. These data show that HMSP represent a useful,  low cost, easily accessible method for administering steroids to mice.","palabras_clave":"pellets|esteroides|prpogestetrona"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64222,"idioma_id":4,"titulo":"Capillary lactic acid validation in an ED.","resumen":"Am J Emerg Med. 2013 Sep;31(9):1365-7. doi: 10.1016\/j.ajem.2013.06.015. Epub 2013 Jul 29.Capillary lactic acid validation in an ED.Seoane L1, Papasidero M, De Sanctis P, Posadas-Martínez LM, Soler S, Rodríguez M.Author informationAbstractINTRODUCTION:One of the most used methods to evaluate patients with a high risk not responding to clinical treatment is the measurement of blood levels of lactic acid (LA). The objective of this study was to compare the sensitivity and specificity of an LA test for capillary and venous blood with LA test for arterial blood in a population of patients with tissue hypoperfusion and to evaluate the time needed for each test.MATERIALS AND METHODS:The following factors were evaluated: the performance of venous and capillary LA in relation to arterial LA, and the time needed to elicit each method from patient admission to mortality according to initial LA.RESULTS:Seventy-nine patients with a median age of 58 years were admitted. The area under the curve for capillary LA was 82% (95% confidence interval [CI], 73-91). The best cutoff point was 2.35, with a sensitivity of 81% (95% CI, 65-90) and a specificity of 70% (95% CI, 53-83). The average time from patient admission until arterial, venous, and capillary LA values were obtained was 112, 117, and 77 minutes, respectively. The patients who died within 3, 30, and 60 days showed an average arterial LA of 5.9, 1.9, and 2.2, respectively.CONCLUSION:The utilization of capillary and venous LA is an effective method of evaluation and risk stratification for patients with different degrees of tissue hypoperfusion. The time needed to elicit capillary LA proved much faster with respect to arterial and venous LA.© 2013 Elsevier Inc. All rights reserved.PMID: 23906620 [PubMed - indexed for MEDLINE]","palabras_clave":"acido lactico|emergencias"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64223,"idioma_id":4,"titulo":"Action of Hexachlorobenzene on tumor growth and metastasis in different experimental models","resumen":"Hexachlorobenzene (HCB) is a widespread organochlorine pesticide, considered a possible human carcinogen. It is a dioxin-like compound and a weak ligand of the aryl hydrocarbon receptor (AhR). We have found that HCB activates c-Src\/HER1\/STAT5b and HER1\/ERK1\/2 signaling pathways and cell migration, in an AhR-dependent manner in MDA-MB-231 breast cancer cells. The aim of this study was to investigate in vitro the effect of HCB (0.005, 0.05, 0.5, 5&#956;M) on cell invasion and metalloproteases (MMPs) 2 and 9 activation in MDA-MB-231 cells. Furthermore, we examined in vivo the effect of HCB (0.3, 3, 30mg\/kg b.w.) on tumor growth, MMP2 and MMP9 expression, and metastasis using MDA-MB-231 xenografts and two syngeneic mouse breast cancer models (spontaneous metastasis using C4-HI and lung experimental metastasis using LM3). Our results show that HCB (5&#956;M) enhances MMP2 expression, as well as cell invasion, through AhR, c-Src\/HER1 pathway and MMPs. Moreover, HCB increases MMP9 expression, secretion and activity through a HER1 and AhR-dependent mechanism, in MDA-MB-231 cells. HCB (0.3 and 3mg\/kg b.w.) enhances subcutaneous tumor growth in MDA-MB-231 and C4-HI in vivo models. In vivo, using MDA-MB-231 model, the pesticide (0.3, 3 and 30mg\/kg b.w.) activated c-Src, HER1, STAT5b, and ERK1\/2 signaling pathways and increased MMP2 and MMP9 protein levels. Furthermore, we observed that HCB stimulated lung metastasis regardless the tumor hormone-receptor status. Our findings suggest that HCB may be a risk factor for human breast cancer progression.","palabras_clave":"hexaclorobenceno|cáncer de mama|crecimiento tumoral|metástasis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64224,"idioma_id":4,"titulo":"Innovation and choice of appropriation mechanisms: evidence from Argentine microdata","resumen":"This paper analyzes the use of mechanisms to appropriate innovation profits in the Argentine industry. Data on around 200 firms belonging to five industrial sectors are used. The appropriability concept is theoretically discussed and contrary to what is widely accepted in innovation literature about the influence of appropriability on innovation efforts here it is argued that characteristics of the innovation process (innovative effort, type of results obtained, accumulated capabilities, cooperation and public support) and structural characteristics (technological intensity, firm size and age) are determinants of appropriation strategy. Probit models are specified and estimated in order to identify the main determinants of the use of each mechanism. Results show that the characteristics of the innovation process indeed influence the appropriation strategy and the use of each mechanism responds to different aspects of innovation process. Descriptive statistics show that the use of strategic mechanisms is higher than the use of legal ones and indicate the existence of different sectoral patterns of appropriation.","palabras_clave":"INNOVATION|APPROPRIABILITY|STRATEGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64227,"idioma_id":5,"titulo":"Desarrollo de un Sellador Post-Ordeño con  Goma Espina Corona","resumen":"Con   la finalidad de evitar infecciones por mastitis en el ganado vacuno, es   recomendable utilizar sellador de pezones post ordeño. El objetivo del   trabajo fue desarrollar un sellador post ordeño antiséptico y con   estabilidad fisicoquímica, para evitar pérdidas del producto por   escurrimiento. El sellador se formuló con la goma espina corona, que   cumple la función de incrementar la viscosidad del producto. Se aplicó   el diseño central compuesto rotable para obtener la formulación óptima.   Se evaluó la viscosidad aparente, como variable de respuesta con un   viscosímetro Brookfield DVII+LV2. Se determinó la fórmula óptima: espina   corona (1,7%), agua desmineralizada (88.60%), glicerina (4,70%),   solución de yodo y tensioactivo (3,00%) y solución buffer pH=5    (2,00%). El sellador mostró un comportamiento reológico del tipo   pseudoplástico. Se concluye que la goma espina corona es apta para   formular un sellador post ordeño, tiene estabilidad fisicoquímica   adecuada y mantiene su principio activo como bactericida.","palabras_clave":"Sellador post-ordeño|goma espina Corona|mastitis|diseño central compuesto rotable"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64228,"idioma_id":4,"titulo":"Sources and distribution of aliphatic and polyaromatic hydrocarbons in sediments from the Neuquen River, Argentine Patagonia","resumen":"Spatial distribution and probable sources of aliphatic and polyaromatic hydrocarbons (AHs, PAHs) were investigated in surfacesediments collected along the bank of the Neuquen River, Argentina. Total concentrations of aliphatic hydrocarbons ranged between0.41 and 125 &#956;g\/g dw. Six stations presented low values of resolved aliphatic hydrocarbons and the n\u2013alkane distribution indexesapplied suggested a clear biogenic source. These values can be considered the baseline levels of aliphatic hydrocarbons for the riversediments. This constitutes important information for the assessment of future impacts since a strong impulse in the exploitation of shale gas and shale oil in these zones is nowadays undergoing. For the other 11 stations, a mixture of aliphatic hydrocarbons of petrogenic and biogenic origin was observed. The spatial distribution reflects local inputs of these pollutants with a significant increase in concentrations in the lower course, where two major cities are located. The highest values of total aliphatic hydrocarbons were found in this sector which, in turn, was the only one where individual PAHs were detected.","palabras_clave":"Neuquen river|Sediments|Hydrocarbon pollution|Biogenic source"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64229,"idioma_id":4,"titulo":"Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination","resumen":"In willow seeds, photooxidative damage is mainly restricted to the outer  cotyledonary tissues, significantly reducing normal germination. Here we  analyzed the damage generated in cotyledonary tissues and investigated  whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons","palabras_clave":"willow seedling|photooxidative damage|cell death|ROS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64230,"idioma_id":5,"titulo":"Comparación de índices de estrés hídrico, a partir de información captada por el sensor MODIS, en la Región Pampeana Argentina.Indexaciones: DIALNET, CIRC, REDALYC, BDDOC CSIC: Sumarios ISOC]","resumen":"La Región Pampeana Argentina manifiesta una tenencia a la expansión agrícola que aumenta los riesgos económicos por su elevada dependencia de las condiciones meteorológicas. A fin de mejorar las predicciones sobre el comportamiento de los cultivos, en este trabajo se estudia la sensibilidad de diferentes índices de estrés hídrico a la disponibilidad de agua en el suelo (DAS), mediante el uso de imágenes satelitales en la mencionada región. Se analizaron tres índices a partir de datos de campañas de terreno en diferentes cultivos, conjuntamente con información satelital. Dos de ellos, Crop Water Stress Index (CWSI) y Water Deficit Index (WDI), combinan información de satélite con datos de estaciones meteorológicas. El tercero, Temperature?Vegetation Dryness Index (TDVI), utiliza solamente información de satélite. Se observó que el TDVI es el que mejor resultados arroja (r2 = 0,92). Se concluye que este índice es el que permite generar diagnósticos adecuados por ser el más sensible a cambios en la DAS.","palabras_clave":"NUEVAS TECNOLOGÍAS AGRICOLAS|SENSORES REMOTOS|INFRARROJO TERMICO|DISPONIBILIDAD DE AGUA EN EL SUELO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64231,"idioma_id":4,"titulo":"Sonochemical synthesis of bis(tri-n-butylstannyl) aromatic compounds via Barbier-like reactions","resumen":"    Abstract  This paper reports a study of the synthesis of  aryltri-n-butylstannanes via a sonochemical Barbier reaction of  aryl- and heteroaryl bromides with bis(tri-n-butyltin)  oxide (2) in THF. Our results  demonstrate that, despite previous reports on contrary, the  aryltributylstannanes can also be obtained under the same reaction conditions via sonicated reactions between aryl  monobromides and tri-n-butyltin chloride (3). A  comparative study of the reactions of electrophiles 2 and 3 with  bromobenzene, 2-bromopyridine, o- and  m-bromoanisole, m-bromotoluene, 9-bromophenanthrene, and 9-bromoanthracene,  indicates that the corresponding aryl- and heteroaryl-tri-n-butylstannyl derivatives are obtained in about the same yields.  Best reaction conditions and the results obtained in the investigation of the  sonicated reactions between several aromatic and heteroaromatic dibromides are  also reported. It was found that the reactions using 1,2-, 1,3-, and  1,4-dibromobenzenes, 4,4?-dibromobiphenyl, 1,4-dibromonaphthalene, 2,5- and  3,5-dibromopyridines, and 2,5-dibromothiophene lead to the corresponding  bis(tri-n-butylstannylated)derivatives  in many cases in very high yields. Also the results obtained in the sonicated  reactions of 1,3,5-tribromobenzene with 2  and 3 are reported.","palabras_clave":"bis(tri-nbutylstannylated)aromatic compounds|sonochemistry|synthesis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64232,"idioma_id":4,"titulo":"Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2012-31 January 2013. Microsatellite marker characterization for the pejerrey, Odontesthes bonariensis, using 454-Roche pyrosequencing technology","resumen":"Pejerrey (Odontesthes bonariensis) is one of the most popular inland fish in South America and has a long history of introductions in many countries. However there are very few genetics tools and information available. Using high-throughput genomic sequencing technology and bioinformatics tools, 3,920 sequences containing microsatellites motifs we identified. A sample of 13 polymorphic microsatellites were isolated and characterized. Wild and hatchery populations were analyzed. Our results suggested that the genetic variability in these populations were significantly different. Monitoring hatchery strains and population genetics studies should be performed for an appropriate genetic resource management.","palabras_clave":"ODONTESTHES BONARIENSIS|PYROSEQUENCING|MOLECULAR MARKERS|WILD AND HATCHERY POPULATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64233,"idioma_id":4,"titulo":"Physico-chemical studies and emulsifying properties of N-propyl-N-methylene  phosphonic chitosan","resumen":"Chitosan is a modified, natural carbohydrate polymer derived by deacetylation of chitin. Due to the presenceof two functional groups can undergo many chemical modifications. In a previous work we describedthe synthetic strategy and characterization of a novel soluble derivative: N-propyl-N-methylene phosphonicchitosan (PNMPC). In the study of some physicochemical properties, results showed that thismodified chitosan aggregates in several steps when the concentration is increased. By addition of NaOHthe initially coiled molecules stretch exposing more phosphonic acid groups to neutralization and finallygive a cooperative reaction with OH(. PNMPC has emulsifying properties and gives O\/W emulsions withquasi-monodisperse small droplets. Emulsions with 0.18% PNMPC and 30:70 o:w ratio exhibited thebest emulsifying properties within the test range. This emulsion ratio showed high stability to long timestorage and several successive freeze\/thaw and heating\/cooling cycles.","palabras_clave":"N-propyl-N-methylene phosphonic chitosan|o\/w emulsions|emulsions stability|chitosan derivative"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64234,"idioma_id":4,"titulo":"Sequences detection of an unbalanced sinusoidal voltage of unknown frequency using a reduced order observer","resumen":"This paper presents a globally convergent nonlinear observer capable of recovering the positive and negative sequences of an unbalanced grid voltage. The proposed algorithm has frequency adaptation, and is therefore, in steady state, immune to grid frequency variations. Another feature is that, since it is a reduced order observer, only three states are needed to implement it. The validity of the proposed scheme is verified through mathematical analysis, simulation and experimental results.","palabras_clave":"Sequences Detection|Adaptive Observer|Reduced Order Observer|Frequency Estimation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64235,"idioma_id":4,"titulo":"Sequence variability in p6 gag protein and gag\/pol co-evolution in Human Immunodeficiency Type-1 subtype F genomes..","resumen":"Polymorphisms occurring at the p6gag protein of HIV-1   have been previously found to have an impact on viral fitness and   antiretroviral (ARV) resistance, mainly on subtype B genomes. We   compared p6gag variability in a large group of 165 subtype F gag-pol sequences, with 36 subtype B sequences from the same study source, and identified sites of gag-pol   coevolution under ARV selection pressure. Subtype-specific differences   in the frequency of point mutations, insertions, and deletions   previously associated with ARV resistance were found. Also, in our   dataset of subtype F genomes a strong association between mutation P5L   in the p1\/p6 cleavage region of gag and the nelfinavir (NFV) resistance   mutation N88DPR was found with no impact on the preference for any of the NFV resistance pathways.","palabras_clave":"HIV-1|GAG|P6"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64236,"idioma_id":4,"titulo":"One-step chemical vapor deposition synthesis of magnetic CNT-hercynite (FeAl2O4) hybrids with good aqueous colloidal stability","resumen":"A multiwall carbon nanotube (MWCNT)\/hercynite (FeAl2O4) hybrid nanomaterial was synthesized  by one-step chemical vapor deposition (CVD) using acetylene as precursor and  FeOx?AlOOH xerogel as catalyst. The hybrid material was composing by hercynite nanoparticles  (diameter 10?50 nm) intimately attached to the walls of the MWCNTs. The diameter  of the MWCNTs was related to particle size of the catalyst. The hybrid nanomaterial exhibited  a characteristic magnetic behavior that can be considered as a combination of superparamagnetism  and ferromagnetism, with a saturation magnetization of 5.7 emu\/g at an  applied field of 18 kOe and a coercivity of 520 Oe. The hybrid displayed a relatively low  pHZPC (approx. 3.2) and formed very stable aqueous suspensions at pH 5.5. Controlled oxidation  of the hybrid generated oxidized functional groups, as ?OH and ?COOH, and promoted  the transformation of hercynite to hematite. Due to the high dispersibility of the  hybrid in water, it presents an interesting potential as nanofiller for hydrophilic polymers","palabras_clave":"chemical vapor deposition|CNT?hercynite hybrids|magnetic CNT  hybrids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64237,"idioma_id":4,"titulo":"HEPATITIS B VIRUS (HBV) AND HEPATITIS D VIRUS (HDV) AMONG BLOOD DONORS FROM ARGENTINA:  CIRCULATION OF VARIANTS WITH NATURAL ANTIVIRAL RESISTANCE","resumen":"        Background . In Argentina, current procedures to ensure safety  of the blood supply for transfusion include the serologic detection of specific  blood borne infections. Objectives.  The aim of this study was to evaluate the prevalence and the genetic diversity  of HBV and HDV among blood donor populations from two distantly located Argentine  regions. Methods. Data from 56,983 blood  donations were analyzed from  Favaloro Foundation of Buenos Aires city (Central region) and from the Central  Blood Bank of Misiones province (Northeast region). Samples reactive for HBsAg  were analyzed for HBV-DNA characterization and HDV serological and molecular  analysis. Results. The HBV prevalence was 0.12% for HBsAg and 1.68% for anti-HBc antibodies  from Buenos Aires, and 0.73%  and  8.55% from Misiones, respectively. Seventy-seven HBsAg reactive samples were analyzed by PCR for  HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires), and F1b and D3  (Misiones) were recorded. Several mutants within the major hydrophilic region  of HBsAg, the reverse transcriptase (associated to natural antiviral  resistance), the basal core promoter and the precore\/core were detected. HDV genotype 1 was identified in Buenos Aires. Conclusions.  This study confirms the circulation of several HBV subgenotypes, as well  as known and newly identified variants, and the presence of HDV1 in this  population. A thorough research has to be carried out to evaluate the clinical  importance of some of the documented mutations as well as those detected in the  HDV1 case.  ","palabras_clave":"HEPATITIS B|HEPATITS D|MISIONES|BUENOS AIRES|VARIANTES |BLOOD DONORS|RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64238,"idioma_id":4,"titulo":"Galectins as New Prognostic Markers and Potential Therapeutic Targets for Advanced Prostate Cancers","resumen":"A better understanding of multi-molecular interactions involved in tumor dissemination is required to  identify new effective therapies for advanced prostate cancer (PCa). Several groups investigated proteinglycan  interactions as critical factors for cross-talk between prostate tumors and their microenvironment.  This review both discusses whether the \u2018galectin-signature\u2019 might serve as a reliable biomarker for the  identification of patients with high risk of metastasis, and assesses the galectin-glycan lattices as potential  novel targets for anti-cancer therapies. The ultimate goal of this review is to convey how basic findings  related to galectins could be in turn translated into clinical settings for patients with advanced PCa.","palabras_clave":"Prostate cancer|Galectins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64239,"idioma_id":5,"titulo":"Modelado para la predicción de enfermedades en cultivos de alto valor comercial","resumen":"Para predecir el comportamiento de enfermedades de plantas mediante la construcción de modelos matemáticos, se evaluó la severidad de manchas foliares ocasionadas por el hongo Alternaria tenuisima en plantaciones de arándano cv. O´Neal en tres localidades: San Pedro, Concordia y Gualeguaychú durante los ciclos epidémicos primavero otoño estivales 2008\/2009 y 2009\/2010. Los mejores modelos simples de regresión logística de respuesta binaria integraron a Snc (grado de senescencia foliar) y a DTxnP (días con temperaturas entre 16 y 36*C) con precisiones de predicción de 93,8 y 78,5% respectivamente. El mejor modelo de respuesta ordinal integró a la interaccón FPr*DTxnP (días con precipitación mayor a 0,2 mm\/día con temperaturas entre 16 y 36*C y a Snc, con una precisión de predicción de 86,2%.","palabras_clave":"epidemiología|modelos predictivos|manejo de enfermedades|arándano alto|Alternaria tenuissima"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64240,"idioma_id":4,"titulo":"Real-time speckle image processing","resumen":"The laser dynamic speckle is an optical phenomenon produced when a laser light is reflected from an illuminated surface undergoing some kind of activity. It allows a non-destructive process for the detection of activities that are not easily observable, such as seed viability, paint drying, bacterial activities, corrosion processes, food decomposition, fruit bruising, etc. The analysis of these processes in real time makes it possible to develop important practical applications of commercial, biological and technological interest. This paper presents a new digital system based on granular computing algorithms to characterize speckle dynamics within the time domain. The selected platform to evaluate the system is Field Programmable Gate Array (FPGA) technology. The obtained minimum clock periods and latencies enable speckle image processing with real-time constraints with a maximum throughput of about thousand 512&nbsp;×&nbsp;512&nbsp;fps.","palabras_clave":"Dynamic speckle|Granular computing|Programmable logic|Real-time processing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64241,"idioma_id":4,"titulo":"Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection","resumen":"Bordetella parapertussis, a close related species of B. pertussis, can also cause the disease named pertussis or whooping cough. The number of cases caused by this related pathogen has risen sustained in the last years. The widely used cellular (wP) or acellular (aP) pertussis vaccines have little or no efficacy against B. parapertussis. In an effort to devise an effective acellular vaccine against B. parapertussis infection, outer membrane vesicles (OMVs) were obtained from B. parapertussis. Proteomic analysis of the resulting OMVs, designated OMVsBpp, evidenced the presence of several surface immunogens including pertactin. The characterized OMVsBpp were used in murine B. parapertussis intranasal challenge model to examine their protective capacity when administered by systemic route. Immunized BALB\/c mice were challenged with sublethal doses of B. parapertussis. Significant differences between immunized animals and the negative control group were observed (p<0.001). OMVsBpp protected against B. parapertussis infection, whereas current commercial aP vaccine showed little protection against such pathogen. More interestingly, protection induced by OMVsBpp against B. pertussis was comparable to our previously designed vaccine consisting in OMVs derived from B. pertussis (OMVsBp). For these experiments we used as a positive control the current commercial aP vaccine in high dose. As expected aP offered protection against B. pertussis in mice. Altogether the results presented here showed that the OMVs from B. parapertussis are an attractive vaccine candidate to protect against whooping cough induced by B. parapertussis but also by B. pertussis.","palabras_clave":"OMV|PARAPERTUSSIS|VACCINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64242,"idioma_id":4,"titulo":"Mechanisms regulating neutrophil survival and cell death","resumen":"Neutrophils not only play a critical role as a firstline of defense against bacteria and fungi infections but alsocontribute to tissue injury associated with autoimmune andinflammatory diseases. Neutrophils are rapidly and massivelyrecruited from the circulation into injured tissues displayingan impressive arsenal of toxic weapons. Althougheffective in their ability to kill pathogens, these weaponswere equally effective to induce tissue damage. Therefore,the inflammatory activity of neutrophils must be regulatedwith exquisite precision and timing, a task mainly achievedthrough a complex network of mechanisms, which regulateneutrophil survival. Neutrophils have the shortest lifespanamong leukocytes and usually die via apoptosis althoughnew forms of cell death have been characterized over thelast few years. The lifespan of neutrophils can be dramaticallymodulated by a large variety of agents such as cytokines,pathogens, danger-associated molecular patterns aswell as by pharmacological manipulation. Recent findingsshed light about the complex mechanisms responsible forthe regulation of neutrophil survival in different physiological,pathological, and pharmacological scenarios. Here, weprovide an updated review on the current knowledge and newfindings in this field and discuss novel strategies that couldbe used to drive the resolution of neutrophil-mediated inflammatorydiseases.","palabras_clave":"NEUTROPHIL|APOPTOSIS|NETOSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64243,"idioma_id":4,"titulo":"Excess fructose intake-induced hypertrophic visceral adipose tissue results from unbalanced precursor cell adipogenic signals","resumen":"We studied the effect of feeding normal adult male rats with a commercial diet (CD) supplemented with fructose added to the drinking water (10% wt\/vol; fructose-rich diet, FRD) on the adipogenic capacity of stromal-vascular fraction (SVF) cells isolated from visceral adipose tissue (VAT) pads. Animals received either the CD or FRD ad libitum for 3 weeks; thereafter, we evaluated the in vitro proliferative and adipogenic capacities of their VAT SVF cells. FRD significantly increased plasma insulin, triglyceride and leptin levels, VAT mass\/cell size, and the in vitro adipogenic capacity of SVF cells. Flow cytometry studies indicated that VAT precursor cell population number did not differ between groups; however, the accelerated adipogenic process could result from an imbalance between endogenous pro- and anti-adipogenic SVF cell signals, clearly shifted towards the former. The increased insulin milieu and its intracellular mediator (insulin receptor substrate-1) in VAT pads, and the enhanced SVF cell expression of Zpf423 and PPAR-&#947;2 (all pro-adipogenic modulators), together with a decreased SVF cell concentration of anti-adipogenic factors (preadipocyte factor-1 and wingless-type MMTV-10b) strongly support this assumption. We hypothesize that the VAT mass expansion recorded in FRD rats results from the combination of initial accelerated adipogenesis and final cell hypertrophy. It remains to be determined whether FRD administration over longer periods of time could perpetuate both processes, or whether cell hypertrophy itself remains responsible for a further VAT mass expansion, as observed in advanced\/morbid obesity. This article is protected by copyright. All rights reserved.","palabras_clave":"abdominal adipose tissue|adipogenic factors|leptin|SVF cells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64244,"idioma_id":4,"titulo":"Impact of Neonatal Manipulation of Androgen Receptor Function on Endocrine-Metabolic  Programming in the Juvenile Female Rat","resumen":"The impact of neonatal androgen receptor (AR) stimulation\/blockage, due to testosterone propionate (TP)\/AR antagonist treatment, on individual anthropometry and neuroendocrine-metabolic function was evaluated in the juvenile female rat. Pups (age 5 days) were s.c. injected with TP (1.25&#8201;mg), flutamide (F; 1.75&#8201;mg), and TP&#8201;+&#8201;F or vehicle (control, CT) and studied on day 30 of age. Body weight (BW), parametrial adipose tissue (PMAT) mass, food intake, adipoinsular axis, and steroidogenic functions were examined. Opposite to TP-rats, F-treated rats developed hypophagia, grew slowly (BW and PMAT), and displayed heightened peripheral insulin sensitivity. These F effects were abrogated in TP&#8201;+&#8201;F animals. Accordingly, TP rats displayed hyperleptinemia, an effect fully prevented by F cotreatment. Finally, androgen-treated animals bore an irreversible ovarian dysfunction (reduced circulating levels of 17HOP4 and ovary 17HOP4 content and P450c17 mRNA abundance). These data indicate that early stimulation of AR enhanced energy store, blockage of AR activity resulted in some beneficial metabolic effects, and neonatally androgenized rats developed a severe ovarian dysfunction. Our study highlights the important role of AR in the early organizational programming of metabolic and neuroendocrine functions.","palabras_clave":"testosterone|ovary"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64245,"idioma_id":5,"titulo":"Recuperacion de Valores Ambientales y Comunitarios en el Parque Provincial Copahue","resumen":"Este artículo expone una experiencia educativa y de extensión en la que  participan alumnos de la carrera de  Geografía y Turismo de la Universidad Nacional del Comahue (Neuquén), quienes impulsaron la realización de tareas de remediación ambiental en un sector de interés turístico del bosque de  Pehuén (Araucaria araucana) dentro un área protegida de la Provincia del Neuquén (Argentina). La situación de riesgo ambiental que afecta a este área es consecuencia de la alteración del delicado equilibrio existente entre los elementos de la naturaleza  por actividades humanas y la falta de control adecuado para proteger el lugar. Además de la intervención sobre el espacio afectado, las actividades incluyeron talleres de concientización, capacitación y difusión a cargo de  alumnos y docentes involucrados.","palabras_clave":"Caviahue|Restauración ambiental|Capacitación|Concientización ambiental"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64246,"idioma_id":4,"titulo":"Modulation of Biotransformation Systems and ABC Transporters by Benznidazole in Rats","resumen":"The effect of antichagasic benznidazole (BZL; 100mg\/kg body weight\/day, 3 consecutive days, intraperitoneally) on biotransfor-mation systems and ABC transporters was evaluated in rats. Expression of cytochrome P-450 (CYP3A), UDP-glucuronosyltrans-ferase (UGT1A), glutathioneS-transferases (alpha glutathioneS-transferase [GST-], GST-, andGST-), multidrug-resis-tance-associated protein 2 (Mrp2), and P glycoprotein (P-gp) in liver, small intestine, and kidney was estimated by Western  blotting. Increases in hepatic CYP3A (30%) andGST-(40%) and in intestinal GST-(72% in jejunumand 136% in ileum) were  detected. Significant increases in Mrp2 (300%) and P-gp (500%) proteins in liver from BZL-treated rats were observedwithout  changes in kidney. P-gp and Mrp2 were also increased by BZL in jejunum (170%and 120%, respectively). In ileum, only P-gp  was increased by BZL (50%). The activities of GST, P-gp, and Mrp2 correlatedwell with the upregulation of proteins in liver and  jejunum. Plasma decay of a test dose of BZL (5mg\/kg body weight) administered intraduodenally was faster (295%) and the area  under the concentration-time curve (AUC) was lower (41%) for BZL-pretreated rats than for controls. The biliary excretion of  BZLwas higher (60%) in the BZL group, and urinary excretion of BZL did not showdifferences between groups. The amount of  absorbed BZL in intestinal sacs was lower (25%) in pretreated rats than in controls. In conclusion, induction of biotransforma-tion enzymes and\/or transporters by BZL could increase the clearance and\/or decrease the intestinal absorption of coadminis-tered drugs that are substrates of these systems, including BZL itself.","palabras_clave":"BENZNIDAZOL|BIOTRANSFORMATION|ABC TRANSPORTERS|RATS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64247,"idioma_id":4,"titulo":"ACETAMINOPHEN INHIBITS INTESTINAL P-GLYCOPROTEIN TRANSPORT ACTIVITY","resumen":"Repeated acetaminophen (AP) administration modulates intestinal P-glycoprotein (P-gp) expression. Whether AP can modulate P-gp activity in a short-term fashion is unknown. We investigated the acute effect of AP on rat intestinal P-gp activity in vivo and in vitro. In everted intestinal sacs, AP inhibited serosal-mucosal transport of rhodamine 123 (R123), a prototypical P-gp substrate. R123 efflux plotted against R123 concentration adjusted well to a sigmoidal curve. Vmax decreased 50% in the presence of AP, with no modification in EC50, or slope, ruling out the possibility of inhibition to be competitive. Inhibition by AP was absent at 0°C, consistent with interference of the active transport of R123 by AP. Additionally, AP showed no effect on normal localization of P-gp at the apical membrane of the enterocyte and neither affected paracellular permeability. Consistent with absence of a competitive inhibition, two further strategies strongly suggested that AP is not a P-gp substrate. First, serosal-mucosal transport of AP was not affected by the classical P-gp inhibitors verapamil or Psc 833. Second, AP accumulation was not different between P-gp knock-down and wild-type HepG2 cells. In vivo intestinal absorption of digoxin, another substrate of P-gp, was assessed in the presence or absence of AP (100 &#956;M). Portal digoxin concentration was increased by 214%, in average, by AP, as compared with digoxin alone. In conclusion, AP inhibited P-gp activity, increasing intestinal absorption of digoxin, a prototypical substrate. These results suggest that therapeutic efficacy of P-gp substrates can be altered if coadministered with AP.","palabras_clave":"Acetaminophen|P-glycoprotein|Interaction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64248,"idioma_id":4,"titulo":"Financialisation vs. Development Finance: The case of the post-crisis Ar- gentine Banking System","resumen":"The end of the Bretton Woods era and the emergence of neoliberal economics resulted in profound transformations in domestic economies and international economic relations. The keystone of these transformations was the liberalisation of domestic and international financial markets. Faulty theoretical foundations and the resulting catastrophic policy failures prompted a vast literature by critical economists on the international financial architecture and domestic financial structures best suited for economic development. In recent decades, debate has also centred on what has been labelled \u201Cfinancialisation\u201D, i.e. the increasingly central role played by financial markets and transactions in economic activity. In this paper we try to ascertain whether Argentina experienced a process of financialisation between 1990 and 2009 and what impact, if any, it has had on Argentine banking. We conclude that there has been a strong process of financialisation in Argentina in the post 2001-2002 crisis period. We also explore in a preliminary fashion potential reasons behind Argentine post-crisis financialisation. The paper concludes by suggesting that it is time to consider alternative banking arrangements if development finance is to become a policy priority.","palabras_clave":"ECONOMIA|NEOLIBERAL|MERCADOS FINANCIEROS|POLITICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64249,"idioma_id":4,"titulo":"Changes of shape memory properties in CuAlNi single crystals subjected to isothermal treatments","resumen":"CuAlNi shape memory alloys are candidates for high temperature applications, mainly for their low diffusion at temperatures higher than room temperature. Ageing thermal treatments made to the &#946;-CuAlNi single crystal phase at 423K were conducted in order to study how the main properties of these alloys change. Ageing of the metastable &#946;-phase above room temperature but well below the eutectoid point can favor a further ordering process started during quenching and trigger the decomposition of part of the alloy into more stable phases. Changes on the thermally and stress induced martensitic transformations were observed after these ageing treatments, modifying the thermal and mechanical response of the material. Modifications of the metastable phase transformation diagrams are considered as well as the pseudoelastic fatigue behavior of the alloys.","palabras_clave":"SHAPE MEMORY ALLOYS|MARTENSITIC TRANSFORMATIONS|PRECIPITATION|ORDERING PROCESSES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64250,"idioma_id":4,"titulo":"Broad MICA\/B expression in the small bowel mucosa: a link between cellular stress and celiac disease","resumen":"The MICA\/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA\/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA\/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA\/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA\/B was mainly intracellularly located in enterocytes. In addition, we identified MICA\/B+ T cells in both the intraepithelial and lamina propria compartments. We also found MICA\/B+ B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA\/B staining in mucosal tissue in severe enteropathy was similar to that found in in vitro models of cellular stress. In such models, MICA\/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA\/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.","palabras_clave":"celiac disease|MICA|mucosal immunology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64251,"idioma_id":4,"titulo":"Structural and vibrational properties of a 1,3,4-oxadiazole derivative with diverse biological activities","resumen":"The 1,3,4-oxadiazole derivatives exhibit  a wide range of biological properties such as antihypertensive, antihelminthic, hipoglysemic, anti-inflammatory and analgesic activies, for these reasons, their study is of biochemical, pharmaceutical and chemical interest. In this work, we studied the structural and vibrational properties of 2-(5-sulfanyl-1,3,4-oxadiazole-2-yl) phenol by infrared spectroscopy and density functional theory (DFT) calculations together with the 6-31 G* and 6-311++G** basis sets. Four stable conformations of the compound were theoretically determined in the gas phase and probably these conformations are present in the solid phase. The harmonic vibrational wavenumbers for the optimized geometries were calculated at the same theory levels. For a complete assignment of the observed bands in the experimental available infrared spectra, the DFT calculations were combined with Pulay\u2019s scaled quantum mechanical force (SQMFF) methodology in orden to fit the theoretical wave numbers values to the experimental ones. Also, the forces constants were calculated in order to observe the changes occurring in the values when they are compared with those obtained for the 5-phenyl-1,3,4-oxadiazole-2-thiol. The presence of the phenol ring linked to the 1,3,4-oxadiazole ring generates low chemical hardness because the HOMO-LUMO gap energy diminished as suggested by the calculated frontier molecular orbitals. Besides, the nature of the of the phenol and 1,3,4-oxadiazole rings and their  topological properties were investigated by means of natural bond orbital (NBO) and by employing Bader\u2019s atoms in molecules theory (AIM), respectively.","palabras_clave":"2-(5-SULFANYL-1,3,4-OXADIAZOLE-2-YL)PHENOL|DFT CALCULATIONS|FORCE FIELD|INFRARED SPECTRURE|VIBRATIONAL SPECTRA|VIBRATIONAL NORMAL MODES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64252,"idioma_id":4,"titulo":"Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity","resumen":"We have previously shown that plasma membrane calcium ATPase (PMCA) pump activity is affected by the membrane protein concentration (Vanagas et al., Biochim Biophys Acta 1768:1641?1644, 2007). The results of this study provided evidence for the involvement of the actin cytoskeleton. In this study, we explored the relationship between the polymerization state of actin and its effects on purified PMCA activity. Our results show that PMCA associates with the actin cytoskeleton and this interaction causes a modulation of the catalytic activity involving the phosphorylated intermediate of the pump. The state of actin polymerization determines whether it acts as an activator or an inhibitor of the pump: G-actin and\/or short oligomers activate the pump, while F-actin inhibits it. The effects of actin on PMCA are the consequence of direct interaction as demonstrated by immunoblotting and cosedimentation experiments. Taken together, these findings suggest that interactions with actin play a dynamic role in the regulation of PMCA-mediated Ca2+ extrusion through the membrane. Our results provide further evidence of the activation?inhibition phenomenon as a property of many cytoskeleton associated membrane proteins where the cytoskeleton is no longer restricted to a mechanical function but is dynamically involved in modulating the activity of integral proteins with which it interacts.","palabras_clave":"PMCA|CYTOSKELETON|ACTIN|REGULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64253,"idioma_id":4,"titulo":"Conformational changes produced by ATP binding to the plasma membrane calcium pump","resumen":"The aim of this work was to study the plasma membrane calcium pump   (PMCA) reaction cycle by characterising conformational changes   associated with calcium, ATP and vanadate binding to purified PMCA. This   was accomplished by studying the exposure of PMCA to surrounding   phospholipids by measuring the incorporation of the photoactivatable   phosphatidylcholine analog [125I]TID-PC\/16 to the protein. ATP could   bind to the different vanadate-bound states of the enzyme either in the   presence or in the absence of Ca2+ with high apparent affinity.Conformational   movements of the ATP binding domain were determined using the   fluorescent analog TNP-ATP. To assess the conformational behaviour of   the Ca2+ binding domain we also studied the occlusion of Ca2+, both in   the presence and in the absence of ATP and with or without vanadate.   Results show the existence of occluded species in the presence of   vanadate and\/or ATP. This allowed the development of a model that   describes the transport of Ca2+ and its relation with ATP hydrolysis.This is the first approach which uses a conformational study to describe thePMCA   P-type ATPase reaction cycle, adding important features to the   classical E1-E2 model devised using kinetics methodology only.","palabras_clave":"P-ATPSE|ATP|REGULATORY FORM|PMCA CYCLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64254,"idioma_id":4,"titulo":"Reference values for volumetric capnography-derived non-invasive parameters in healthy individuals","resumen":"The aim of this study was to determine typical values for non-invasive volumetric capnography (VCap) parameters for healthy volunteers and anesthetized individuals. VCap was obtained by a capnograph connected to the airway opening. We prospectively studied 33 healthy volunteers 32 ± 6 years of age weighing 70 ± 13 kg at a height of 171 ± 11 cm in the supine position. Data from these volunteers were compared with a cohort of similar healthy anesthetized patients ventilated with the following settings: tidal volume (VT) of 6-8 mL\/kg, respiratory rate 10-15 bpm, PEEP of 5-6 cmH&#8322;O and FiO&#8322; of 0.5. Volunteers showed better clearance of CO&#8322; compared to anesthetized patients as indicated by (median and interquartile range): (1) an increased elimination of CO&#8322; per mL of VT of 0.028 (0.005) in volunteers versus 0.023 (0.003) in anesthetized patients, p < 0.05; (2) a lower normalized slope of phase III of 0.26 (0.17) in volunteers versus 0.39 (0.38) in anesthetized patients, p < 0.05; and (3) a lower Bohr dead space ratio of 0.23 (0.05) in volunteers versus 0.28 (0.05) in anesthetized patients, p < 0.05. This study presents reference values for non-invasive volumetric capnography-derived parameters in healthy individuals. Mechanical ventilation and anesthesia altered these values significantly.","palabras_clave":"VOLUMTRIC CAPNOGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64255,"idioma_id":5,"titulo":"ESTUDIO EXPLORATORIO DE LA INGESTA Y PREVALENCIA DE DEFICIENCIA DE VITAMINA D EN MUJERES  DE 65 AÑOS QUE VIVEN EN SU HOGAR FAMILIAR O EN RESIDENCIAS PARA AUTOVALIDOS DE LA CIUDAD DE BUENOS AIRES, ARGENTINA","resumen":"El estado nutricional y factores socioambientales influyen sobre la salud y calidad de vida del adulto mayor.  Ingestas inadecuadas de proteínas, calcio y vitamina D  afectan la salud ósea.   Objetivos: 1) Evaluar el aporte de energía, proteínas,  calcio y vitamina D en mujeres &#8805; 65 años; 2) Analizar  según el lugar de residencia: hogar familiar (HF) o residencias semicautivas (RSC); 3) Evaluar la relación entre  ingesta y parámetros bioquímicos.   Población: 44 mujeres ambulatorias y clínicamente  sanas de (X ± DE) 75 ± 7 años, índice de masa corporal 28 ±  4 kg\/m2  .  Métodos:1) Cuestionarios de frecuencia de consumo de  alimentos, exposición solar y nivel socioeconómico. 2)  Laboratorio: En suero: 25-hidroxivitamina D (25OHD),  crosslaps (CTX), calcio (Cas), fósforo y fosfatasa alcalina  ósea e Índice calcio\/creatinina (Cau\/Cru) en orina de 2 h.   Resultados: El grupo total presentó ingestas inferiores  a las recomendadas excepto en proteínas, con déficit  mayor en RSC. El 88 % presentó deficiencia de vitamina  D (25OHD < 20 ng\/ml). Se halló correlación positiva entre  25OHD e ingesta de vitamina D (r = 0,46; p < 0,007) y  correlación negativa entre 25OHD y CTX en aquellas con  niveles < 15 ng\/ml (r = -0,51; p < 0,03). Los niveles de  25OHD, Cas y Cau\/Cru fueron mayores en HF que RSC.  Conclusión: La alta prevalencia de déficit de vitamina  D, ingesta inadecuada de calcio y vitamina D en mujeres  añosas constituye un factor de riesgo para la salud ósea.  Se requieren programas de educación alimentaria y eventual suplementación con vitamina D enfatizados en grupos de mayor riesgo como RSC.","palabras_clave":"INGESTA DE CALCIO|DEFICIENCIA VITAMINA D|ADULTOS MAYORES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64256,"idioma_id":5,"titulo":"Osteomalacia Tumoral","resumen":"La osteomalacia tumoral (OT) es un síndrome paraneoplásico en el cual el paciente presenta dolor óseo, fracturas y debilidad muscular. Es producido principalmente por una elevación de los niveles en sangre del factor de crecimiento fibroblástico 23 (FGF-23). En la OT, el FGF-23 es secretado por tumores mesenquimales, los cuales se caracterizan por ser de tamaño pequeño y de lento crecimiento. El FGF-23 actúa a nivel de los túbulos renales afectando la reabsorción de fósforo, lo cual conduce a hipofosfatemia y a bajos niveles de 1,25-dihidroxivitamina D [1,25(OH)2D3]. El tratamiento de la OT es la resección del tumor. Debido a las características de estos tumores muchas veces no pueden ser localizados o son quirúrgicamente irresecables y requieren tratamiento médico a largo plazo, consistente en sales de fósforo y calcitriol. La adherencia al tratamiento muchas veces resulta difícil debido a las reacciones secundarias, así como también a las complicaciones asociadas.","palabras_clave":"hipofosfatemia|osteomalacia tumoral|factor de crecimiento fibroblástico 23"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64257,"idioma_id":4,"titulo":"Plasmid vectors and molecular building blocks for the development of genetic manipulation tools for Trypanosoma cruzi","resumen":"The post genomic era revealed the need for developing better performing, easier to use and more sophisticated genetic manipulation tools for the study of Trypanosoma cruzi, the etiological agent of Chagas disease. In this work a series of plasmids that allow genetic manipulation of this protozoan parasite were developed. First of all we focused on useful tools to establish selection strategies for different strains and which can be employed as expression vectors. On the other hand molecular building blocks in the form of diverse selectable markers, modifiable fluorescent protein and epitope-tag coding sequences were produced. Both types of modules were harboured in backbone molecules conceived to offer multiple construction and sub-cloning strategies. These can be used to confer new properties to already available genetic manipulation tools or as starting points for whole novel designs. The performance of each plasmid and building block was determined independently. For illustration purposes, some simple direct practical applications were conducted.","palabras_clave":"Trypanosoma cruzi|Chagas Disease|Genetic manipulation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64258,"idioma_id":5,"titulo":"Actualización sobre irrigantes y  nuevas técnicas de irrigación utilizados para la eliminación del smear layer o barro dentinario","resumen":"La terapia endodóntica consiste en la eliminación completa de la pulpa que ha sufrido un daño irreversible y de todo el tejido remanente, limpieza, conformación y obturación del sistema del conducto radicular, de manera que se pueda conservar el diente como una unidad funcional dentro del arco dental. El éxito de la terapia endodóntica depende, en primer término, de la limpieza y conformación del sistema de conductos radiculares, y esto se lleva a cabo mediante el procedimiento conocido como Preparación Biomecánica,  denominado también preparación químico-quirúrgica, de limpieza y conformación, o simplemente instrumentación.En este proceso, se utilizan instrumentos endodónticos y soluciones químicas auxiliares para la irrigación, lo cual hace  necesario seleccionar sustancias que tengan la capacidad de eliminar tanto las sustancias orgánicas como las inorgánicas. Durante la limpieza y conformación del conducto, se generan restos orgánicos (pulpa y predentina) y restos inorgánicos (limallas dentinarias), que se depositan sobre las paredes del conducto y forman una capa de barro dentinario (BD) o smear layer. En conductos infectados pueden encontrarse también microorganismos.   Esta capa está químicamente formada por dos fases: la primera orgánica, compuesta por residuos de colágenos dentinarios y glicosaminoglicanos y que sirve de base la segunda fase que es predominantemente inorgánica y que está compuesta por dos capas distintas y superpuestas; la primera delgada no adherente y fácil de remover que cubre las paredes de los conductos, y la segunda que ocluye los túbulos y se adhiere fuertemente a las paredes del conducto. Hasta el momento hay diferentes opiniones respecto a su conservación o eliminación. Los que están a favor de mantenerla se basan en que éste actuaría de barrera mecánica obstruyendo los túbulos e impidiendo el pasaje de microorganismos Sin embargo, la tendencia actual considera remover el barrillo dentinario, con el fin de  lograr reducir el número de microorganismos que puedan estar presentes en él,  eliminar los restos orgánicos que sirven de sustrato para el crecimiento y desarrollo bacteriano, aumentar la permeabilidad dentinaria optimizando la desinfección del conducto al permitir una mejor acción del irrigante y de la medicación tópica. Se ha demostrado que las sustancias irrigadoras no remueven el contenido inorgánico adosado a las paredes, por lo que se ha visto la necesidad de utilizar sustancias quelantes que tengan la capacidad de atrapar los iones inorgánicos, con el fin de obtener un sistema de conductos adecuadamente limpio que pueda estar en contacto íntimo con el material de obturación. El hipoclorito de sodio es la sustancia proteolítica más comúnmente utilizada para la irrigación de los conductos radiculares, debido a sus excelentes propiedades. A pesar de esto es incapaz de disolver la sustancia inorgánica que se encuentra en el barro dentinario,  dificultando de esta manera su eliminación. Es por ello que se recomienda su utilización en combinación con alguna sustancia quelante, de las cuales una de la más utilizadas es el ácido etilendiaminotetraacético (EDTA) al 17 %. El objetivo de este trabajo es realizar una revisión en la literatura científica de los últimos años acerca de los diferentes irrigantes disponibles para la terapia endodóntica; sus características, propiedades y mecanismos de acción, con el fin de establecer cuál de ellos es el más apropiado para la eliminación del barro dentinario, y el que proporciona mejor desinfección durante la instrumentación del conducto radicular.","palabras_clave":"AGENTES IRRIGANTES|TERAPIA PULPAR|BARRO DENTINARIO|SMEAR LAYER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64259,"idioma_id":4,"titulo":"Toll- like receptor 4 orchestrates neutrophil recruitment into airways during  the first hours of Bordetella pertussis infection","resumen":"The lipo-oligosaccharide (LOS) of Bordetella pertussis is a key modulator of the host immune response, mainly due to the role of its lipid A moiety in Toll-like receptor 4 (TLR4)-mediated signaling. Most of previous results on B. pertussis LOS were obtained when the bacteria had already been established within the host. Here, we found that this molecule participates in host-bacterial interaction as major determinant, very early in the course of bacterial infectious process. Within the first 2 hours of the infection, genes encoding the pro-inflammatory mediators TNF-alpha, IL-6, and IL-1&#61538; and the chemokines CXCL1, CXCL2 and CXCL5 known to participate in neutrophil-mediated mucosal defense were induced through TLR4 signaling. Moreover we observed that TLR4 signaling is absolutely needed to promote a rapid recruitment of neutrophils into the airways. Interestingly, the depletion of those neutrophils abolished the clearance of B. pertussis, thereby exacerbating lung infection. In summary, we determined that TLR4 is a central player in the orchestration of very early neutrophil-dependent innate defense against B. pertussis.","palabras_clave":"TLR4|Bordetella|neutrophil|innate response"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64260,"idioma_id":4,"titulo":"Sclerosing peritonitis, a rare complication after intestinal transplant. Report of one case successfully treated with adjustment of immunosuppression.","resumen":"Sclerosing peritonitis is a complication described in different clinical situations, such as patients that underwent prolonged peritoneal dialysis or renal transplantation with previous history of peritoneal dialysis. The origin of this entity is unclear so far and it is believed that several mechanisms may contribute to its development. The hallmark of sclerosing peritonitis is the continuous accumulation of fibrocollagenous deposits in the intestinal wall and mesenteries causing progressive adhesion of the intestinal loops and mesenteric retraction resulting in intestinal obstruction. Also, it has been described as a rare complication after intestinal transplant that might lead to graft failure. In this report, we describe a case of sclerosing peritonitis after intestinal transplantation that was successfully treated with modifications in the immunosuppressive regime allowing restitution of gastrointestinal transit and intestinal autonomy.","palabras_clave":"intestinal transplant|sclerosing peritonitis|chronic rejection|eosinophils"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64261,"idioma_id":4,"titulo":"Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response","resumen":"The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.","palabras_clave":"CCL20|luciferase reporter|transgenic mice|liver"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64262,"idioma_id":5,"titulo":"La familia como espacio de realizacion de los derechos del niño","resumen":"La primera infancia es un momento crítico. Por un lado, se encuentra atravesada por la mayor vulnerabilidad que resulta inherente a la condición de niños pequeños, y al mismo tiempo constituye una etapa de la vida en que el acceso a una nutrición adecuada, un entorno saludable y seguro, la educación y el juego se tornan aspectos claves para el desarrollo futuro.En la provisión de cuidados físicos, atención emocional y orientación cuidadosa, los padres u otros cuidadores, con quienes los niños establecen los vínculos más fuertes, son los principales actores. De esta manera, la familia se torna el espacio por excelencia para la realización de los derechos del niño.Esto da lugar a una serie de reflexiones centradas en la familia y en la inscripción de la familia en el entramado institucional de la sociedad.","palabras_clave":"DERECHOS DEL NIÑO|PRIMERA INFANCIA|RESPONSABILIDAD PARENTAL|RESPONSABILIDAD ESTATAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64263,"idioma_id":4,"titulo":"Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen","resumen":"Here, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+rNbHsp90.3 group) and the fusion of MBP to rNbHsp90.3 (MBP-rNbHsp90.3 group). The covalent linkage between NbHsp90.3 and MBP to bring a fusion protein was essential to induce the strong specific antibody response with predominance of IgG2a. Eighty-four days after the first immunization, splenocyte proliferation from MBP-rNbHsp90.3-immunized mice was consistently higher than that from MBP and MBP+rNbHsp90.3 groups. In addition, splenocytes from MBP-rNbHsp90.3 immunized mice produced higher levels of IFN-&#61543;&#61472;than controls. Finally, both formulations with rNbHsp90.3 significantly enhanced the MHC class I expression levels, but only rNbHsp90.3 covalent bound to MBP induced a specific cellular immune response against MBP measured as increased percentage of CD8+ T cells. Taken together, these results suggest that plant HSP90s could be incorporated as adjuvants in vaccines that require the generation of a Th1 response along with a CD8 cytotoxic cell response to confer immunity.","palabras_clave":"plant Hsp90|Immunity|Adjuvant|Vaccine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64264,"idioma_id":4,"titulo":"Exploration for Triatoma virus (TrV) infection in laboratory-reared triatomines  of Latin America: a collaborative study","resumen":"Triatoma virus (TrV) is a small, non-enveloped virus that has a þssRNA genomeand is currently classified under the Cripavirus genus of the Dicistroviridae family. TrVinfects haematophagous triatomine insects (Hemiptera: Reduviidae), which are vectors ofAmerican trypanosomosis (Chagas disease). TrV can be transmitted through thehorizontal faecal?oral route, and causes either deleterious sublethal effects or even thedeath of laboratory insect colonies. Various species of triatomines from different regions ofLatin America are currently being reared in research laboratories, with little or noawareness of the presence of TrV; therefore, any biological conclusion drawn fromexperiments on insects infected with this virus is inherently affected by the side effects ofits infection. In this study, we developed a mathematical model to estimate the sample sizerequired for detecting a TrV infection.We applied this model to screen the infection in thefaeces of triatomines belonging to insectaries from 13 Latin American countries, carryingout the identification of TrV by using RT-PCR. TrV was detected in samples coming fromArgentina, which is where the virus was first isolated from Triatoma infestans (Hemiptera:Reduviidae) several years ago. Interestingly, several colonies from Brazil were also found infected with the virus. This positive result widens the TrV?s host range to a total of14 triatomine species. Our findings suggest that many triatomine species distributed overa large region of South America may be naturally infected with TrV.","palabras_clave":"DICISTROVIRIDAE|TRIATOMA VIRUS|SAMPLING MODEL|INSECTARY CONTAMINATION|PATHOGEN TRANSMISSION|CHAGAS DISEASE|RED TRV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64265,"idioma_id":5,"titulo":"La chía (Salvia hispanica L.) como fuente de alimentos bioactivos para la elaboración de alimentos","resumen":"En el presente trabajo se estudió el proceso de extracción de aceite de chía mediante CO2 supercrítico como una alternativa a los procesos convencionales, así como también se evaluaron las principales características fisicoquímicas de los aceites obtenidos. En virtud de las características del aceite de chía, el mismo podría ser utilizado para mejorar la relación de ´cidos grasos, omega-6:omega-3 -cuyo desbalance se relaciona actualmente con las dietas occidentales-, aportando además, otros compuestos de interés nutricional. Si bien la composición acídica y el contenido de sus compuestos minoritarios le confieren al aceite de chía cualidades benéficas desde el punto de vista nutricional, el alto tenor de ácidos grasos poliinsaturados determina su baja estabilidad oxidativa por lo cual, es muy importante el desarrollo de estrategias que contribuyan a lograr su adecuada conservación. Se investigó la influencia de diferentes parámetros sobre la conservación del aceite de chía (temperatura y tiempo de almacenamiento, adición de antioxidantes). Además, se obtuvieron harinas de chía y fracciones con alto contenido de fibra dietética, evaluando sus propiedades fisicoquímicas y funcionales. La información obtenida sugiere que los subproductos de chía (aceites, harinas residuales, fracciones ricas en fibra dietaria) podrían constituir ingredientes de potencial relevancia para ser aplicados en la elaboración de alimentos funcionales debido a la presencia de compuestos bioactivos con efectos potenciales en la prevención de algunas enfermedades crónicas no transmisibles.","palabras_clave":"SEMILLA DE CHIA|ACEITE DE CHIA|ACIDOS GRASOS OMEGA 3 Y OMEGA 6|COMPUESTOS BIOACTIVOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64266,"idioma_id":4,"titulo":"Microestructure, chemical composition and mucilage exudation of chia (Salvia hispanica L.) nutlets from Argentina","resumen":"BACKGROUND: The micromorphology and anatomy of nutlets, myxocarpy (mucilage exudation) and mucilage structure of Argentinean chia were described using scanning electron microscopy (SEM). The proximal composition of nutlets and mucilage was also studied.RESULTS: Chia nutlets are made up of a true seed and a pericarp enclosing the seed; they are small, glabrous, elliptic and apically rounded. The pericarp has cuticle, exocarp, mesocarp and bone cells vertically arranged and endocarp. The myxocarpy was carefully recorded by SEM. After 5 min in contact with water, the cuticle of nutlets is broken and the exocarp cell content gradually surrounds the rest of the nutlet. The proximal composition of chia nutlets was studied; fat is the major component (327 ±8.0 g kg&#8722;1) followed by protein (293 ±4.0 g kg&#8722;1) and &#64257;ber (276 ±1.0 g kg&#8722;1). Extractions of chia nutlets with water at room temperature yielded 38 ±1.0 g kg&#8722;1 (dry basis) of mucilage. The fresh mucilage structure was similar to a network of open pores. The freeze-dried crude mucilage contained more ash, residual fat and protein than commercial guar and locust bean gum. The solubility of 10.0 g L&#8722;1 w\/v solution of chia freeze-dried crude mucilage in water increased with temperature, being maximal at 60 &#9702;C (870 g kg&#8722;1).CONCLUSION: The results obtained show a fast exudation of chia mucilage when nutlets are in contact with water. The freeze-dried crude mucilage hydrates easily in water, even at low temperatures. Chia nutlets have mucilaginous substances, with interesting functional properties from a technological and physiological point of view.","palabras_clave":"SALVIA HISPANICA L.|MUCILAGE|MORPHOLOGY|SEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64267,"idioma_id":4,"titulo":"High Prevalence of Substance Use Among Men Who have Sex with Men in Buenos Aires, Argentina: Implications for HIV Risk Behavior","resumen":"Abstract: Five hundred gay and other men who have sexwith men (G&amp;MSM) from Buenos Aires, Argentina completedan assessment regarding substance use and sexualbehavior. During the past 2 months, 78 % of participantsconsumed alcohol and 61 %drugs.Over 20 %of participantsreporting alcohol, marijuana, cocaine sulfate, or tranquilizeruse, did so daily. Heavy alcohol use was more likely amongparticipants with greater mood reactivity (AOR = 1.64) andless likely among those who identified as gay (AOR = 0.38).Weekly drug use was less likely among older (AOR = 0.98),and gay-identified participants (AOR = 0.50), but morelikely among participants with greater mood reactivity(AOR = 1.49). Drug use was correlated with unprotectedanal and vaginal intercourse with men, women, and transvestitesamong non-gay identified participants (r = 0.22).Findings highlight the need to reduce substance use and sexualrisk behavior in this population.","palabras_clave":"Gay men|Drug use|Alcohol use|Latin America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64268,"idioma_id":4,"titulo":"Involvement of KLF14 and egr-1 in the TGF-beta1 action on Leydig cell proliferation","resumen":"Involvement of KLF14 and egr-1 in the TGF-beta1 action on Leydig cell proliferation     1Gonzalez CR, 2Vallcaneras SS, 3Calandra RS, 3,4Gonzalez Calvar SI      Transforming growth factor beta1 (TGF-beta1) is a pleiotropic cytokine that modulates cell homeostasis. In Leydig cells, TGF-beta1 exerts stimulatory and inhibitory effect depending on the type I receptor involved in the signaling pathway. The aim of the present work was to study the signaling mechanisms and the intermediates involved in the action of TGF-beta1 on TM3 Leydig cell proliferation in the presence or absence of progesterone.   The MTT assay showed that the presence of progesterone in the culture Media lead to a proliferative effect that was blocked by Ru486, an inhibitor of progesterone receptor; and ALK-5 did not participate in this effect.   TGF-beta1 (1ng\/ml) increased the expression of p15 (an inhibitor of cell cycle) in TM3 Leydig cells, and this effect was blocked by progesterone (1 µ M). The expression of PCNA presented a higher increase in the cell cultured with TGF-beta1 plus progesterone than in cells cultured only with TGF-beta1.   Progesterone induced the gene expression of endoglin, a cofactor of TGF-beta1 receptor that leads to a stimulatory signaling pathway, despite of the absence of progesterone response element in endoglin gene. In addition, the presence of progesterone induced the gene expression of egr-1 and also KLF14, indicating that this steroid channels the signaling pathway into a non-canonical mechanism. In conclusion, these findings suggest that the proliferative action of TGF-beta1 involves endoglin. This co-receptor might be induced by KLF14 which is probably activated by progesterone.","palabras_clave":"TGF-beta1|LEYDIG|PROLIFERATION|KLF-14"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64269,"idioma_id":5,"titulo":"Preámbulo para las lecturas y reflexiones acerca del espacio. Lo construido en el relato","resumen":"En este artículo reflexionamos en torno a la manera en que algunos espacios son construidos en los relatos de los actores y actoras sociales que los habitan y transitan. Nos interesa especialmente recorrer, junto con las descripciones e impresiones que estos testimonios nos brindan acerca de esos espacios, algunas pistas sobre el modo en que estos relatos dialogan, por un lado, con construcciones de sentidos previas de los espacios -redes de preconceptos hegemónicos acerca de un lugar que marcan de antemano las condiciones de inteligibilidad- y, por otro lado, las concepciones que se van elaborando a partir del habitar mismo de esos espacios.  Este ejercicio tiene el fin de observar de manera cuidadosa nuestro proceder etnográfico y crítico a la hora de aproximarnos al campo e intentar establecer un diálogo con las significaciones espaciales construidas por los sujetos y sujetas como parte importante de sus experiencias.","palabras_clave":"Espacialidad|Relatos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64270,"idioma_id":4,"titulo":"On the use of crystal vibrational modes in the estimation of the anisotropic displacement parameters of hydrogen atoms in molecular crystals: para-Nitroaniline as a test case","resumen":"The use of crystal vibrational modes and frequencies calculated for the periodic system to complement a Translation Libration Screw (TLS) fit in the estimation of anisotropic displacement parameters (ADPs) of hydrogen atoms in molecular crystals is proposed. As a test case we have used the para-nitroaniline crystal, for which a reference set of ADPs has been obtained by performing a single crystal neutron diffraction study at 100 K. Although the largest difference between estimated and experimental reference values amounts to 0.06 Å^2, this value being about six times the experimental uncertainty, the agreement is better than three experimental uncertainties for 33 out of the total of 36 ADPs. The performance of the suggested method, particularly for the amino atoms, is thoroughly analyzed.","palabras_clave":"ANISOTROPIC DISPLACEMENT PARAMETERS|PERIODIC CALCULATION|MOLECULAR CRYSTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64271,"idioma_id":4,"titulo":"MARCKS protein is phosphorylated and regulates calcium mobilization during human acrosomal exocytosis","resumen":"      Acrosomal  exocytosis is a calcium-regulated exocytosis that can be triggered by PKC  activators. The involvement of PKC in acrosomal exocytosis has not been fully  elucidated, and it is unknown if MARCKS, the major substrate for PKC,  participates in this exocytosis. Here, we report that MARCKS is expressed in  human spermatozoa and localizes to the sperm head and the tail. Calcium- and phorbol ester-triggered acrosomal  exocytosis in permeabilized sperm was abrogated by different anti-MARCKS  antibodies raised against two different domains, indicating that the protein participates  in acrosomal exocytosis. Interestingly, an anti-phosphorylated MARCKS antibody  was not able to inhibit secretion. Similar results were obtained using recombinant  proteins and phospho-mutants of MARCKS effector domain (ED), indicating that  phosphorylation regulates MARCKS function in acrosomal exocytosis. It is known  that unphosphorylated MARCKS sequesters PIP2. This phospholipid is the  precursor for IP3, which in turn triggers release of calcium from  the acrosome during acrosomal exocytosis. We found that PIP2 and adenophostin,  a potent IP3-receptor agonist, rescued MARCKS inhibition in  permeabilized sperm, suggesting that MARCKS inhibits acrosomal exocytosis by sequestering  PIP2 and, indirectly, MARCKS regulates the intracellular calcium  mobilization. In non-permeabilized sperm, a permeable peptide of MARCKS ED also  inhibited acrosomal exocytosis when stimulated by a natural agonist such as  progesterone, and pharmacological inducers such as calcium ionophore and phorbol  ester. The preincubation of human sperm with the permeable MARCKS ED abolished  the increase in calcium levels caused by progesterone, demonstrating that  MARCKS regulates calcium mobilization. In addition, the phosphorylation  of MARCKS increased during acrosomal  exocytosis activation by the same activators. Altogether, these  results show that MARCKS is a negative modulator in acrosomal exocytosis, probably by  sequestering PIP2, and it is phosphorylated during acrosomal  exocytosis.  ","palabras_clave":"MARCKS|HUMAN SPERM|ACROSOMAL EXOCYTOSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64272,"idioma_id":4,"titulo":"Native trees of the Northeast Argentine. Natural hosts of the Cryptococcus neoformans -Cryptococcus gattii species complex","resumen":"Background: In Argentina, information about epidemiology and environmental distribution of  Cryptococcus is scarce. The city of Resistencia borders with Brazil and Paraguay where this fungus is  endemic. All this supported the need to investigate the ecology of the genus and the epidemiology of  cryptococcosis in this area.  Aims: The aim was to investigate the presence of species of Cryptococcus neoformans - Cryptococcus  gattii complex and their genotypes in trees of the city of Resistencia.  Methods: One hundred and five trees were sampled by swabbing technique. The isolates were  identified using conventional and commercial methods and genotyped by PCR-RFLP (Restriction  Fragment Length Polymorphism).  Results: Cryptococcus was found in 7 out of the total trees. 6 out of 7 Cryptococcus isolates were  identified as C. neoformans and one as C. gattii. C. gattii was isolated from Grevillea robusta. C.  neoformans strains were isolated from Tabebuia avellanedae and Peltophorum dubium. Genotyping  showed that all C. neoformans belonged to the VNI type and C. gattii belonged to the VGI type.  Conclusions: This represents the first study on the ecology of Cryptococcus spp. associated to trees  from northeastern Argentina, and the first report describing Grevillea robusta as a host of members of  this fungal genus. Another finding is the isolation of C. neoformans from Tabebuia avellanedae and  Peltophorum dubium, both tree species native to northeastern Argentina.","palabras_clave":"C. gattii|ecological niche|genotype|Grevillea robusta|Peltophorum dubium|Tabebuia avellanedae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64273,"idioma_id":5,"titulo":"Prevalencia de especies de Malassezia asociadas a lesiones de dermatitis seborreica en pacientes de Argentina","resumen":"Antecedentes La dermatitis seborreica (DS) es considerada la segunda dermatosis más frecuentemente asociada al género Malassezia, pero poco es el conocimiento que se tiene sobre la epidemiología de esta asociación. Objetivos Conocer la prevalencia de las especies de Malassezia asociadas a DS y analizar su distribución según la localización de las lesiones. Materiales y métodos Se estudiaron 226 muestras de piel de pacientes con lesiones compatibles con DS. Edad, sexo y sitio anatómico de las lesiones fueron registrados. Las cepas fueron identificadas por PCR-RFLP. Resultados Se obtuvieron 131 cultivos positivos, de los cuales se aislaron 141 cepas ya que en 10 cultivos se detectó asociación de 2 especies. M. globosa (43,3%) fue la especie más frecuente, seguida por M. furfur (20,6%), M. sympodialis (17,0%) y M. restricta (16,3%). Se obtuvieron 3 aislamientos de M. slooffiae (2,1%) y uno de M. pachydermatis (0,7%). M. globosa presentó relación significativa (p &lt; 0,05) con cuero cabelludo y M. restricta se aisló solo en zonas de la cabeza. Conclusiones Nuestros resultados muestran a M. globosa como la especie más relacionada con DS. La prevalencia de las otras especies es diferente a la informada por otros autores. Solo M. globosa y M. restricta presentaron un patrón de relación con la localización de la lesión. Es destacable el aislamiento de M. pachydermatis por ser primariamente zoofílica. La ecología del género Malassezia y el rol patogénico de sus especies se encuentran todavía en estudio. Este trabajo es una contribución a este conocimiento","palabras_clave":"DERMATITIS SEBORREICA|MALASSEZIA|EPIDEMIOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64274,"idioma_id":4,"titulo":"Promoter sequence of Shiga Toxin II (Stx2) is recognized in vivo leading to the production of biologically active Stx2","resumen":"Shiga toxins (Stxs) are the main agent responsible for the development of hemolytic uremic syndrome (HUS), the most severe and life-threatening systemic complication of infection with enterohemorrhagic Escherichia coli (EHEC) strains. We previously reported Stx2 expression by eukaryotic cells after they were transfected in vitro with the stx2 gene cloned into a prokaryotic plasmid (pStx2). The aim of this study was to evaluate whether mammalian cells were also able to express Stx2 in vivo after pStx2 injection. Mice were inoculated by hydrodynamic based transfection (HBT) with pStx2. We studied the survival, the percentage of polymorphonuclear leukocytes in plasma, plasma urea levels and histology of the kidney and the brain of mice. Mice displayed a lethal dose-response to pStx2. Stx2-mRNA was recovered from the liver and Stx2 cytotoxic activity was observed in plasma of mice injected with pStx2. Stx2 was detected by immunofluorescence in the brains of mice inoculated with pStx2, and markers of central nervous system (CNS) damage were observed, including increased expression of glial fibrillary acidic protein (GFAP) and fragmentation of NeuN in neurons. Moreover, anti-Stx2B immunized mice were protected against pStx2 inoculation. Our results show that Stx2 is expressed in vivo from the wild stx2 gene, reproducing pathogenic damage induced by purified Stx2 or secondary to EHEC-infection.","palabras_clave":"SUH|stx2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64275,"idioma_id":5,"titulo":"Relevamiento sanitario en caprinos en una zona de peri-valle de la provincia de Río Negro, Argentina","resumen":"de Río Negro. Tras la revisación clínica, se tomaron muestras de materia fecal para análisis coproparasitológicos y muestras de sangre para la detección de anticuerpos  contra Brucella sp, Leptospira sp, Toxoplasma gondii, Virus de Artritis Encefalitis Caprina, Virus de Parainfluenza-3 Bovino, Herpesvirus Bovino 1, Pestivirus Bovino y Virus Respiratorio Sincicial Bovino. Cada productor fue encuestado para determinar sus conocimientos, actitudes y prácticas (CAP) sobre el manejo sanitario. Al examen clínico se observó un 40% de animales con condición corporal baja, un 48,3% con pediculosis y un 11,6% con secuelas de ectima contagioso. Los hpg fueron negativos o muy bajos y en 5 de los 6 establecimientos se detectó Fasciola hepatica. Dos animales (3,3%) resultaron positivos a Brucella sp, 7 (11,3%) a Leptospira Pomona, 12 (20%) a T.gondii, 53 (88,3%) presentaron títulos de anticuerpos neutralizantes para Virus Respiratorio Sincicial bovino, 46 (76,6%) a Virus Parainfluenza-3 bovino, 8 (13,3%) a Herpesvirus bovino 1 y todos resultaron negativos al Virus de Artritis Encefalitis y Pestivirus. Del estudio CAP se desprende que los productores conocen en promedio un 42% (rango 26-56%) de las enfermedades más frecuentes en la región, ninguno aplica vacunas y desparasitan en forma sintomática.","palabras_clave":"PATAGONIA|CABRAS|ENFERMEDADES|ESTUDIO CAP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64276,"idioma_id":4,"titulo":"Fabry disease peripheral blood immune cells release inflammatory cytokines: role of globotriaosylceramide","resumen":"Fabry disease is an X-linked lysosomal disorder (LD) due to deficiency   of the enzyme &#945;-galactosidase A (&#945;Gal), which leads to the accumulation   of neutral glycosphingolipids, mainly globotriaosylceramide (Gb3).   Several mechanisms contribute to the diverse physiopathological   alterations observed in this disease, and it has been suggested that an   underlying proinflammatory state could play a significant role. The aim   of this study is to investigate the presence of a proinflammatory state   in the different subsets of peripheral blood mononuclear cells (PBMC)   and to understand the mechanisms that contribute to its onset and   perpetuation. We have shown that cultured PBMC from Fabry patients   present a higher proinflammatory cytokine expression and production.   Moreover, we determined that among PBMC, dendritic cells and monocytes   present a basal proinflammatory cytokine production profile, which is   further exacerbated with an inflammatory stimulus. Finally we   established that normal, monocyte-derived dendritic cells and   macrophages display the same proinflammatory profile when cultured in   the presence of Gb3 and an inhibitor of &#945;Gal. Furthermore, this effect   can be abolished using a TLR4 blocking antibody, indicating that TLR4 is   necessary in the process. In summary, our results demonstrate the   presence of a proinflammatory state involving two key subsets of innate   immunity, and provide direct evidence of Gb3 having a proinflammatory   role, likely mediated by TLR4, a finding that could help in the   understanding of the underlying causes of the inflammatory pathogenesis   of Fabry disease.","palabras_clave":"FABRY DISEASE|PROINFLAMMATORY CYTOKINE|GLOBOTRIAOSYLCERAMIDE|MACROPHAGE|DENDRITIC CELL|TLR4"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64277,"idioma_id":4,"titulo":"Molecular phylogenetic relationships of the LIolaemus rothi complex and a new species of lizard from auca mahuida volcano (squamata liolaemini)","resumen":"A new species of lizard of the genus Liolaemus from Neuquén Province, western Argentina, is described. The new species is a member of the Liolaemus rothi species complex, and mitochondrial and nuclear molecular data show it as sister taxon of the clade composed of (L. hermannunezi (L. tromen + L. loboi)), differing in size, squamation, coloration, and sexual dimorphism from the other species of this group. Liolaemus sitesi sp. nov. has a dark body coloration with series of notched blotches on the dorsum, with bright spots, and a very iridescent yellow-green coloration in natural light.Liolaemus sitesi sp. nov. is found only in the Auca Mahuida volcano and is terrestrial, dwelling on the stony slopes with sandy soil between 1300 m and the volcano summit.","palabras_clave":"Patagonia|Liolaemus sitesi|rothi complex|phylogeny|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64278,"idioma_id":4,"titulo":"Comparison of transient and stable expression of foot-and-mouth disease virus capsid proteins in mammalian cells","resumen":"Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production. A plasmid containing the capsid precursor P12A and protease 3C sequences of foot-and-mouth disease virus (FMDV) was constructed and used to compare transient and stable expression in mammalian cells. When BHK-21 cells were transfected with the re-combinant vector, protease 3C cleaved the capsid precursor P12A into the structural proteins VP0, VP1 and VP3. A sucrose gradient demonstrated that the structural proteins assembled into different subviral particles. Attempts to generate a stable cell line only allowed isolating low-level-expressing clones, probably due to the effect of protease 3C on the cells. Moreover, the recombinant protein yield achieved in transient expression assays was much higher than the one achieved in stable expression assays. Results in-dicate that mammalian cells are a good strategy to produce recombinant FMDV subviral particles. However, the alternative approach of transient gene expression in scalable systems should be used instead of the standard method that involves the generation of a stable cell line.","palabras_clave":"VIRUS DE LA FIEBRE AFTOSA|PROTEÍNAS RECOMBINANTES|CÉLULAS DE MAMÍFERO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64279,"idioma_id":5,"titulo":"Una experiencia de articulación entre el colegio secundario y la universidad. Taller de Matemática: Un puente entre el colegio Secundario y la Universidad","resumen":"En esta comunicación se relata una experiencia de articulación entre el nivel secundario y la universidad, a través de un Taller de Matemática denominado Un puente entre el colegio secundario y la Universidad. Dicho taller se llevó a cabo en el Centro Regional Bariloche de la Universidad Nacional del Comahue, y estuvo destinado a los alumnos de 5to y 6to año de los diferentes colegios secundarios de la ciudad de Bariloche. El Curso estuvo organizado en tres ejes: Eje 1: Lenguaje, pensamiento y método matemático, Eje 2: Funciones, Eje 3: Pensamiento Numérico. Se optó por la modalidad de Taller como metodología de trabajo tendiente a favorecer la actividad creadora y reflexiva de los estudiantes. El objetivo principal de la propuesta es suministrar a los estudiantes de nivel secundario una primera instancia de acercamiento a la universidad tanto a su espacio físico como al abordaje de contenidos matemáticos.","palabras_clave":"ARTICULACION|SECUNDARIA|UNIVERSIDAD |MATEMATICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64280,"idioma_id":4,"titulo":"Relationship of cortisol and life events to the metabolic syndrome in men","resumen":"Psychological factors and stressful life events (LE) are considered to play a role in the onset of the metabolic syndrome (MS). We tested the association between LE and cortisol, a marker of chronic stress, with the risk of developing MS and their interaction. From a total number of 2906 men who completed a screening for the early detection of prostate cancer, 149 healthy men (mean&nbsp;¡À&nbsp;SD age, 58.6&nbsp;¡À&nbsp;7.7 years) were included in this study. Participants were assessed by the Holmes and Rahe questionnaire about their experience of LE during the previous 1¨C5 years. MS was diagnosed according to National Cholesterol Education Program-Adult Treatment Panel III (ATP-III) and International Diabetes Federation (IDF) criteria. Serum cortisol was measured at 08:00¨C09:00&nbsp;h. Participants with MS (IDF criteria) reported significantly more past LE (p&nbsp;=&nbsp;0.009) and greater summed weight of LE (p&nbsp;=&nbsp;0.049) than those without MS. Furthermore, LE interacted with cortisol in relation to MS: in men with increased serum cortisol levels (&nbsp;¡Ý&nbsp;13.7&nbsp;¦Ìg\/dl), number of LE significantly predicted MS-status (relative risk (RR) =&nbsp;1.16, p&nbsp;=&nbsp;0.03), whereas in men with low cortisol, LE were unrelated to MS (p&nbsp;=&nbsp;0.52). We conclude that LE were significantly more prevalent in men with the MS than without the MS, according to IDF criteria, independent of the effects of age and body mass index, especially in men with increased serum cortisol levels","palabras_clave":"ABDOMINAL OBESITY|CORTISOL|LIFE EVENTS|STRESS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64281,"idioma_id":4,"titulo":"Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field","resumen":"A sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples was designed. This drug was originally developed for the treatment of respiratory pathologies. However, in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growth and lipid degradation. The drug residues accumulated in animal tissues represent a potential risk for the human health. Subsequently, the development of a fast and simple method for the CL quantification employed to detect its abuse in livestock production is an attractive analytical challenge.    The proposed method was based on the use of a screen printed carbon electrode (SPCE) modified with multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs) previously incorporated by electrodeposition process. The MWCNTs-CH\/AuNPs\/SPCE was characterized by cyclic voltammetry, X-ray diffraction and scan electron microscopy.    With the aim to carry out the CL determination, a pre-concentration procedure was required. For this purpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinity support to capture the drug present in the sample. This support was exposed to a desorption process. Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensor exhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL&#8722;1. The detection and quantification limits obtained were 0.003 and 0.01 ng mL&#8722;1, respectively with an analysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained by the proposed sensor were successful.","palabras_clave":"Voltammetric sensor|Nanostructured platform|Anabolic drug|Food safety"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64282,"idioma_id":4,"titulo":"Risk Analysis in renewable Energy: Assessment of The Vulnerability of the Environment  and Community","resumen":"As a result of recent accidents, society is becoming more conscious and concerned about the risk that are under to theenvironment and communities. A proposal of assessment of the vulnerability of the environment and community is presented hereunder a holistic perspective using fuzzy logic as a formal tool. An internal factor of the risk of a system under a given threat isassessed. Risk is considered as the union between the threat and the vulnerability of the environment and community. This approachshows that the reduction in either or both of them makes a reduction in the risk content as well. The main purpose of this proposal isnot to obtain a single value as output; in fact the result is a map which can contribute to the different areas of the community indecision making.","palabras_clave":"RISK|THREAT|VULNERABILITY|SOFT AND HARD SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64283,"idioma_id":5,"titulo":"Plantas utilizadas en cercos vivos de ciudades patagónicas: aportes de la etnobotánica para la conservación","resumen":"Los cercos vivos son parte significativa del paisaje de las ciudades de la Patagonia y pueden ser muy importantes no sólo como reservorios de biodiversidad sino también como posibles fuentes de invasiones. En este trabajo se estimó la riqueza de especies, sus familias botánicas y el origen biogeográfico de las plantas seleccionadas como cercos vivos en ocho ciudades turísticas de la Patagonia. Si se considera la influencia que los colonos europeos han tenido sobre el desarrollo cultural y paisajístico de la región, es esperable unaproporción elevada de recursos de origen Holártico. Se identificaron las especies en 100 casas elegidas al azar por localidad (San Martín de los Andes, Villa la Angostura, Dina Huapi, El Bolsón, Lago Puelo, Esquel y Trevelin) y 300 para Bariloche dado el mayor tamaño de la ciudad. La riqueza total correspondió a 137 especies, y las más comunes fueron: Cytisus scoparius (L.) Link (9%), Rosa sp. (8%), Pseudotsuga menziesii (Mirb.)Franco (7%), Cupressus macrocarpa Hartw. ex Gordon (5%) y Ligustrum sinense Lour. (3%). De las 48 familias botánicas representadas, las más frecuentes fueron: Rosaceae, Fabaceae, Cupressaceae y Pinaceae (67% del total del registro). Las especies provienen de la región Holártica (72%), de la Antártica (23%), Paleotropical (19%), Neotropical (5%) y Australiana (2%). Se concluye que los cercos estudiados muestran en términos de riqueza de especies, similitudes con las principales especies ornamentales a nivel global. Sin embargo, la presencia de elementos antárticos (32 especies) indicaría la incorporación de especies nativas de los bosques andino-patagónicos en los criterios locales, por ende, con un valioso papel a nivel de la conservación. Por otra parte, el relevamiento muestra que ciertas especies pueden representar riesgos de invasiones biológicas (e.g., Sorbus aucuparia L. Crataegus monogyna Jacq., Ulex europaeus L.), por lo que se propone la difusión de sus efectos negativos en la población.","palabras_clave":"leñosas|invasiones|exóticas|nativas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64284,"idioma_id":5,"titulo":"El rol de los musgos en la germinación de especies leñosas: Implicancias de la heterogeneidad de micro-sitios para la restauración","resumen":"Los musgos cumplen un rol importante en los ecosistemas. Son pioneros en suelos inestables, controlan la erosión, colonizan sitios alterados, y actúan como reservorio de agua, esto último es sustancial para las plantas vasculares de los bosques templados en la época estival de mayor déficit hídrico. El objetivo de este trabajo fue analizar la riqueza de musgos que se desarrollan sobre el suelo en sectores de bosque nativo con diferente grado de alteración, su capacidad de retención de agua y su papel como facilitadores de la germinación de especies leñosas. El estudio se realizó en un área bajo restauración en la Reserva Nacional Lago Puelo. Se muestrearon tres sectores distintos: a) área remanente de bosque maduro de Nothofagus dombeyi, b) bosque secundario de Austrocedrus chilensis y c) matorral dominado por especies exóticas. Se registraron las especies de musgos sobre el suelo y sus formas de vida. Se analizó la capacidad de retención de agua de los musgos y la facilitación en la germinación de Maytenus boaria durante la primavera. Los resultados evidencian una reducción de especies de musgos presentes a mayor grado de alteración. Se encontraron diferencias significativas entre los valores de retención de agua de los musgos (515%) con respecto del suelo sin musgos (46%). La germinación de semillas de Maytenus boaria se vio facilitada por la presencia de musgos en el sustrato. Se resalta el papel de los musgos como reservorio de agua a microescala, facilitando el establecimiento de plántulas. Estos resultados demuestran la factibilidad de utilizar los musgos del bosque nativo como material en actividades de restauración","palabras_clave":"BIODIVERSIDAD|BRIÓFITAS|CONSERVACIÓN|HETEROGENEIDAD DE MICRO-SITIOS|MANEJO|PROPAGACIÓN|RESTAURACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64285,"idioma_id":4,"titulo":"MYH9-related disease: five novel mutations expanding the spectrum of causative mutations and confirming genotype\/phenotype correlations","resumen":"MYH9-related disease (MYH9-RD) is a rare autosomal dominant syndromic disorder caused by mutations in MYH9, the gene encoding for the heavy chain of non-muscle myosin IIA (myosin-9). MYH9-RD is characterized by congenital macrothrombocytopenia and typical inclusion bodies in neutrophils associated with a variable risk of developing sensorineural deafness, presenile cataract, and\/or progressive nephropathy. The spectrum of mutations responsible for MYH9-RD is limited. We report five families, each with a novel MYH9 mutation. Two mutations, p.Val34Gly and p.Arg702Ser, affect the motor domain of myosin-9, whereas the other three, p.Met847_Glu853dup, p.Lys1048_Glu1054del, and p.Asp1447Tyr, hit the coiled-coil tail domain of the protein. The motor domain mutations were associated with more severe clinical phenotypes than those in the tail domain","palabras_clave":"MYH9 mutation|inherited thrombocytopenias|platelets"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64286,"idioma_id":5,"titulo":"Las competencias éticas en los estudiantes universitarios","resumen":"A lo largo de este escrito hemos intentado visibilizar diversas problemáticas del modelo universitario actual que se convierten en obstáculos a sortear teniendo como horizonte un avance en materia de formación ética de los futuros profesionales que devenga en una mejor atención de las demandas sociales vigentes al momento del ejercicio profesional. Sin profesionales comprometidos social y éticamente en los contextos de trabajo no es posible hablar de verdadera formación universitaria.","palabras_clave":"educacion|competencias|universidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64287,"idioma_id":4,"titulo":"TRANSLATIONAL PROGRAM ON NEURONAL CEROID LIPOFUSCINOSES (NCLs) IN LATIN AMERICA AS A MODEL EXPERIENCE ON RARE DISEASE STUDIES","resumen":"INTRODUCTION: Rare Diseases (RDs) are more than 6,900 pathologies that affect fewer than 200,000 people in US, or less than 5 \/10,000 in the EU. The overall prevalence in Latin-America remains unknown. A Translational Program for the study of Neuronal Ceroid Lipofuscinoses (NCLs) in Latin-America runs along the last 10 years at the Children´s Hospital in Córdoba-Argentina. METHODOLOGY: The key traits for the integrated study of NCLs were implemented, including diagnoses, phenotypes\/genotypes, care\/palliative medicine, education\/social aspects, promoting at the same time families advocacy. High technology inputs were enabled through international collaboration. RESULTS: A unified study algorithm allowed effective clinical\/morphological\/biochemical and molecular diagnostic studies with CLN1\/PPT1 and CLN2\/TPP1 enzyme testing in Dried Blood Spots\/saliva\/leukocytes, morphological studies, and mutational analysis. In 33 families from 4 countries 20 novel and 11 known mutations were described. Forty two individuals were diagnosed for 7\/14 forms of NCLs. CLN2 was stated as the most frequent form, showing two markedly differentiated subtypes, classic Late Infantile and variant Juvenile. CONCLUSIONS: The Program-approach should be considered as an effective model in Latin-America for other RDs. It is conduced to coordinate and support diagnoses and research, with the potentiality to facilitate drug and biologic products development for treatments. Capacity building and implementation of educational programs is still needed in the region. The 14º NCL International Congress will be held in Córdoba-Argentina, October 22-26th 2014 as an opportunity to spread out the up to date scientific knowledge on NCLs in our region.","palabras_clave":"TRANSLATIONAL PROGRAM |NEURONAL CEROID LIPOFUSCINOSES |RARE DISEASE |LATIN AMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64288,"idioma_id":4,"titulo":"Phenotypic subgroups of Neuronal Ceroid lipofuscinosis type CLN2 in Latin America","resumen":"The Neuronal Ceroid Lipofuscinoses (NCLs) are inherited neurodegenerative lysosomal pathologies in childhood,characterized by the pathological accumulation of ceroid-lipofuscin in the brain and extra-cerebral tissues. The CLN2\/TPP! gene encodes the lysosomal enzime Tripeptidyl-Peptidase 1 (TPPI). The high phenotypic variability of CLN2 mutated genotypes was not explained yet. Aims: To establish phenotype\/genotype correlations in classic late infantile (LI) and variant juvenile (J) forms.  Methods: 1) Clinical studies; 2) Evaluation of TPP1 activity; 3) Transmission electron microscopy; 4) Screening of mutations and polymorphims; 5) 'In silica' mutation studies.  Results: 26 CLN2 individuals showed LI (65,4%), or J (34,6%) phenotypes. TPP1 deficiencies: n=11, null activity; n=7, unknown. Morphology: pure curvilinear bodies (CBs), n=16; mixed CBs, n=2; fingerprints, n=1; not performed, n=7. CLN2 mutations, n=15; n=7 novel; n=8 worldwide known. The mutations c.827A>T(p.Asp276Val-exon7) in homozigosis was associated to the LI phenotype. The intronic mutations c.887-10A>G(intron 7) and c.89+5G>C(intron2), in heterozygosis, were associated to the J phenotype.  Discussin and Conclusions: The J form, rarely mentioned in the literature, appeared in more than the 30% patiens, usually associated with residual TPP1 activity. The most frecuent mutations in South America were described, and those frequently associated with each CLN2 phenotype were recognized.","palabras_clave":"CLN2                               |NEURONAL CEROID  LIPOFUSCINOSES|PHENOTYPIC      |LATIN AMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64289,"idioma_id":4,"titulo":"A NOVEL CLN8 MISSENSE MUTATION UNDERLIES VARIANT LATE INFANTILE NEURONAL CEROID LIPOFUSCINOSIS IN SOUTH AMERICA","resumen":"Neuronal Ceroid Lipofuscinosis (NCL) is a group of inherited neurodegenerative diseases that may affect individuals of any age, characterized by accumulation of lipofuscin-like lipopigments in neurons and peripheral tissues. CLN8 is one of the 13 classified forms, caused by mutations in CLN8 gene and may present with 2 possible phenotypes: Epilepsy Progressive with Mental Retardation (EPMR) or variant Late Infantile (vLI). To date, neither both variants nor CLN8 mutations were described in Latin America. The change c.685C&gt;G, p.Pro229Ala, was found in 2 individuals from Argentina and Mexico, however, it had not yet been validated as a mutation. In this work, we aim to analyze by molecular approaches the CLN8 gene in individuals suspected of CLN8 and validate the change c.685C&gt;G and all eventually found DNA variations.","palabras_clave":"CLN8|MUTATION|NEURONAL CEROID LIPOFUSCINOSIS |SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64290,"idioma_id":5,"titulo":"Uso de hábitat por aves en rellenos sanitarios del noreste de la provincia de 1 Buenos Aires, Argentina.","resumen":"Los basurales a cielo abierto y los rellenos sanitarios (RS) son sitios de atracción para diferentes especies 19 de aves oportunistas que pueden aumentar sus poblaciones debido al suplemento alimentario brindado por los residuos orgánicos. Estas aves pueden actuar como vectores de enfermedades o contaminantes, causar daños a la agricultura, aumentar el riesgo de accidentes aéreos y tener efectos negativos sobre otras especies por competencia o predación. Los objetivos de este trabajo son identificar y cuantificar las especies de aves y el uso de hábitat en tres RS del noreste de la provincia de Buenos Aires, y determinar la importancia de la cantidad de residuos depositados sobre dichas variables durante dos períodos invernales consecutivos. La abundancia total de aves fue diferente entre los RS en ambos períodos de estudio, aunque el de las gaviotas fue siempre el grupo más abundante en los tres RS. El RS Norte 3 (RSN3) tuvo la mayor abundancia de gaviotas y palomas. Las aves zancudas fueron significativamente más abundantes en el RS González Catán, y las rapaces tuvieron abundancias similares en los tres RS en ambos períodos. En ambos períodos las gaviotas de los tres RS fueron abundantes en todos los ambientes, mientras que sólo la abundancia de las palomas en el RSN3 estuvo relacionada con las celdas de disposición final y el borde cercano. Se encontró relación entre la superficie de residuos expuestos y la abundancia total de aves y la abundancia de palomas, que a su vez está relacionado con la cantidad diaria de residuos depositados. La reducción de la porción orgánica y el manejo de los residuos durante la disposición final, el manejo de los ambientes que rodean a los sitios de disposición final, junto a métodos directos pasivos y activos de control, reduciría las abundancias de aves en los RS y la potencialidad de los efectos negativos para el hombre y el ambiente.","palabras_clave":"aves y residuos|gaviotas|palomas|ambientes disturbados|antropización y avifauna|rellenos sanitarios"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64291,"idioma_id":4,"titulo":"Age-dependent effects of prenatal stress on the corticolimbic dopaminergic system development in the rat male offspring","resumen":"      We have previously demonstrated that prenatal stress (PS) exerts an  impairment of midbrain dopaminergic (DA) system metabolism especially after puberty, suggesting a particular  sensitivity of DA development to variations in gonadal hormonal peaks.  Furthermore we demonstrated that PS alters the long term androgens profile of  the rat male offspring from prepubertal to adult stages. In this work we evaluated  the sexual hormones activational effects on the DA system by analysing PS  effects on the dopaminergic D2-like (D2R) and on the gonadal hormones receptor levels on cortical and  hippocampal areas of prepubertal and adult male offspring. We further evaluated  the dendritic arborization in the same areas by quantifying MAP2 immunoexpresion.  Our results show that PS affected oestrogen receptor alpha (ERa) expression:  mRNAer1s and ER a protein levels were decreased on prefrontal cortex and  hippocampus of adult offspring. Moreover, PS reduced D2R protein levels in hippocampus  of prepubertal rats. Morphological studies revealed that prepubertal PS rats presented decreased MAP2 immunoexpression  in both areas suggesting that PS reduces the number of dendritic arborizations.  Our findings suggest that PS exerts long-term effects on the DA system by  altering the normal connectivity in the areas, and by modulating the expression  of D2R and ERa in an age-related pattern. Since the developing forebrain DA  system was shown to be influenced by androgen exposure, and PS was shown to disrupt  perinatal testosterone surges, our results suggest that prenatal insults might be affecting the organizational role of androgens  and differentially modulating their activational role on the DA development.  ","palabras_clave":"Prenatal restraint stress|D2R|MAP2|Oestrogen receptor alpha"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64292,"idioma_id":4,"titulo":"The dentition of Manidens condorensis (Ornithischia; Heterodontosauridae) from the Jurassic Can?ado´n Asfalto Formation of Patagonia: morphology, heterodonty and the use of statistical methods for identifying isolated teeth","resumen":"The recently described Manidens condorensis is one of the most completely known taxa of the family Heterodontosauridae from the southern landmasses. However, some dental aspects are not well known due to preservational problems in the type material. This contribution reports new isolated teeth found in the Can &#771;ado &#769;n Asfalto Formation (Early-Middle Jurassic). These teeth are referred to Manidens condorensis based on the presence of autapomorphic characters of the unusual dentition of this taxon, such as the highly asymmetric tooth crowns and small crenulations on each denticles. The isolated crowns are well preserved and reveal the presence of undescribed and new autapomorphical features, including apical and basal wear facets on the occlusal surface of isolated crowns and a wear surface also in the caniniform tooth. We carried out statistical analyses (including morphogeometrical and discriminant analyses), using the holotype crowns as a morphological starting point, for characterising shape variation of the crowns along the toothrow and for identifying the position of isolated crowns. These analyses allow defining morphological regions within the postcaniniform toothrow and produce a metrically based discriminant function to predict the hypothetical position of future discoveries, providing a methodological framework that could be applied to other extinct heterodont dinosaurs.","palabras_clave":"Dinosauria|Ornithischia|Jurásico|dentición"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64293,"idioma_id":4,"titulo":"Antifungal and antiaflatoxigenic activity by vapour contact of three essential oils and effects of environmental factors on their efficacy","resumen":"The present investigation reports the antifungal and antiaflatoxigenic properties of essential oils (EOs)  from boldo (Pëumus boldus Mol.), poleo (Lippia turbinata var. integrifolia Griseb., clove (Syzygium aromaticum  L.) and from boldo and poleo mixtures present in the headspace of peanut extract medium at  three water activity levels (aW) (0.98, 0.95 and 0.93). Moreover, the ability of boldo and poleo oils to  maintain their antifungal activity was evaluated after subjecting them to environmental variations. Boldo  EO at doses 1500 mL\/L showed a highly significant effect on Aspergillus section Flavi lag phase (>300 h),  growth rate (93e100% of inhibition) and aflatoxin B1 (AFB1) accumulation (100% of inhibition) at all aW  levels assayed. The antifungal and antiaflatoxigenic effects of poleo and clove EOs were highly dependent  on aW. In general boldoepoleo oil mixtures showed less inhibitory activity on Aspergillus strains than the  boldo pure oil. The antifungal ability of volatile components released by boldo and poleo EOs was stable  against temperature changes; while it was reduced when poleo was stored during six months and when  boldo was exposed to sunlight and UV.  Boldo and poleo EO volatile fractions can be used as effective non-toxic biopreservatives in stored  peanut industry against AF contamination.","palabras_clave":"Essential oils|Aflatoxins|Fumigant activity|Aspergillus flavus|A. parasiticus|Peanut|Environmental factors"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64294,"idioma_id":5,"titulo":"Consumo de Alimentos Fuera del Hogar en Argentina. Relevancia de la Composición Demográfica y tipología de los Hogares\u201D","resumen":"  Con el crecimiento economico experimentado por Argentina en los ultimos anos se produjeron cambios en los patrones de consumo, incluyendo el de alimentos fuera del hogar (AFH). Si bien el poder adquisitivo ha sido indicado como un factor determinante, otras caracteristicas de los hogares y sus interrelaciones han sido tambien propuestas como relevantes sin que se haya profundizado su estudio. Para cubrir esa laguna, el objetivo del presente trabajo es conocer la relacion entre el tamano, la composicion y el tipo de hogar sobre el consumo de AFH para el periodo 2004-2005, con datos de la Encuesta Nacional de Gastos de Hogares 2004\/05. El analisis se aplica a la provincia de Santa Fe, en Argentina, una de las de mayor importancia economica y demografica del pais. Metodologicamente se aplica un modelo Logit para utilizar sus predichos y los efectos de cada variable sobre la probabilidad de compra. Los resultados indican la relevancia que tiene el tamano, medido en terminos de cantidad de miembros; la composicion en funcion de grupos etarios y genero, asi como el tipo de hogares. Tambien denotan diferencias en los patrones de consumo segun las caracteristicas analizadas. Se concluye sobre la relevancia de tomar en cuenta los resultados obtenidos para el diseno de programas y politicas especificas, tales como las de prevencion de la salud, control de la obesidad y nutricion infantil.","palabras_clave":"Calidad de Vida|Género|Políticas Públicas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64295,"idioma_id":4,"titulo":"Functional traits, the phylogeny of function, and ecosystem service vulnerability.","resumen":"People depend on benefits provided by ecological systems. Understanding how these ecosystem services ? and the ecosystem properties underpinning them ? respond to drivers of change is therefore an urgent priority. We address this challenge through developing a novel risk-assessment framework that integrates ecological and evolutionary perspectives on functional traits to determine species? effects on ecosystems and their tolerance of environmental changes. We define Specific Effect Function (SEF) as the per-gram or per capita capacity of a species to affect an ecosystem property, and Specific Response Function (SRF) as the ability of a species to maintain or enhance its population as the environment changes. Our risk assessment is based on the idea that the security of ecosystem services depends on how effects (SEFs) and tolerances (SRFs) of organisms ? which both depend on combinations of functional traits ? correlate across species and how they are arranged on the species? phylogeny. Four extreme situations are theoretically possible, from minimum concern when SEF and SRF are neither correlated nor show a phylogenetic signal, to maximum concern when they are negatively correlated (i.e., the most important species are the least tolerant) and phylogenetically patterned (lacking independent backup). We illustrate the assessment with five case studies, involving both plant and animal examples. However, the extent to which the frequency of the four plausible outcomes, or their intermediates, apply more widely in real-world ecological systems is an open question that needs empirical evidence, and suggests a research agenda at the interface of evolutionary biology and ecosystem ecology.","palabras_clave":"Functional traits|Ecosystem services|phylogeny|exttinction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64296,"idioma_id":4,"titulo":"Parasitism and physiological trade-offs in stressed capybaras","resumen":"Parasites play a key role in regulating wildlife population dynamics, but their impact on the host appears to be contextdependent. Evidence indicates that a synergistic interaction between stress, host condition and parasites is implicated in this phenomenon, but more studies are needed to better understand this context-dependency. With the goal to assess the net effect of two types of chronic stress on various host-parasite interactions, we conducted an experiment in capybaras to evaluate the impact of food restriction and physical restraint on the infection intensity of specific gastrointestinal nematodes and coccidia, and how these stressors affected the growth, body condition, and some immuno-physiological parameters. Our hypothesis was that both forms of stress would result in an alteration in the host-parasite interactions, with deteriorated condition and reduced immunological investment leading to high parasite burdens and vice versa. Stressed capybaras had significantly higher coccidia infection intensities; but among individuals that were smaller, those stressed consistently showed lower helminth burdens than controls. Both stress treatments had a marked negative impact on growth and body condition, but concomitantly they had a significant positive effect on some components of the immune system. Our results suggest, on the one hand, that during prolonged periods of stress capybaras preventatively invest in some components of their immunity, such as innate humoural defenses and cells that combat helminths, which could be considered a stress-dependent prophylaxis. On the other hand, stress was found to cause greater infection intensities of protozoans but lower burdens of nematodes, indicating that the relationship between stress, physiological trade-offs and infection depends on the type of parasite in question. Moreover, both findings might be related in a causal way, as one of the immunological parameters enhanced in stressed capybaras is associated with the immune response to control helminths.","palabras_clave":"vicious circle|host-parasite relationship|stress-dependent prophylaxis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64297,"idioma_id":4,"titulo":"Breeding Potential of Elite Maize Landraces to Improve Forage Yield and Quality of Two Heterotic Patterns","resumen":"Breeders recognize that the maize (Zea mays L.) germplasm base is  narrow and consequently, it should be extended by the introgression of exotic  germplasm. Several studies have explored the potential of open-pollinated  populations to improve grain yield, disease resistances, and other traits, but  few have considered traits related to forage yield and quality. In the present  study, a group of elite landraces were evaluated as sources of favorable  alleles to improve two target hybrids representing two heterotic patterns,  Stiff Stalk Synthetic × Lancaster Sure Crop (B73 × Mo17) and Stiff Stalk  Synthetic × Argentine Orange Flint (B73 × LP122-2). Inbred lines B73, Mo17, and  LP122-2, single crosses B73 × Mo17 and B73 × LP122-2, and 24 inbred × landrace  crosses were evaluated in four environments for stover dry matter yield (SY),  ear dry matter yield (EY), in vitro digestibility of stover dry matter (iDS),  and in vitro digestibility of ear dry matter (iDE). Several landraces are a  potential source of favorable alleles not present in the parent inbred lines of  both target hybrids for SY, EY, and iDE. ARZM17035 is the most promising  landrace since it has favorable alleles for all traits not present in both  target hybrids. The results indicate that landraces represent a potential  source of useful genetic variability to improve forage maize.","palabras_clave":"maize|forage|breeding|genetic variability"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64298,"idioma_id":4,"titulo":"Transport Properties of hydrogenated ZnO microwires","resumen":"We have studied the magnetoresistance (MR) of hydrogen plasma-treated pure ZnO wires of tensof micrometer diameter at different temperatures. A negative MR of 1% at 8 T applied field ismeasured for all wires at 4 K, independent of the temperature (300 K . . . 773 K) used during thehydrogen treatment. However, a positive MR develops, the higher the treatment temperature.The MR can be explained with a semiempirical model taking into account local magnetic momentsand the s?d exchange interaction. These results together with field anisotropy in the MR indicate theappearance of magnetic order due to the hydrogen treatment in agreement with recently publishedreports on the influence of hydrogen in bulk ZnO single crystals. Hydrogen doping may providea way to trigger defect-induced magnetism in small oxide structures.","palabras_clave":"MAGNETORESISTANCE|ZINC OXIDE|DEFECT-INDUCED|WIRES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64299,"idioma_id":4,"titulo":"'Isolation and identification of entomopathogenic fungi and their evaluation against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in stored maize'.","resumen":"The objective of this study was to isolate and  identify native entomopathogenic fungi from different  components of maize agroecosystem, and evaluate their  virulence against Tribolium confusum, Rhyzopertha dominica,  and Sitophilus zeamais, three insect pest vectors of  aflatoxigenic fungi. Paecilomyces and Metarhizium were  the most abundant genera isolated from the soil. Identification  of fungal cultures by DNA extraction, amplification,  and sequencing showed that all isolates macro- and  micromorphological identified as P. lilacinus were Purpureocillium  lilacinum. The isolate JQ926223 showed the  lowest LT50 for T. confusum (4, 66 days) and R. dominica  (9, 38 days), and the isolate JQ926212 demonstrated similar  LT50 for the three insects evaluated between the range  of 11, 7 to 14, 95 days. Maximum mortality rate was  observed for the isolate JQ926223. The isolates of Purpureocillium  lilacinum JQ926223 and JQ926212 may be  considered good candidates for biologic control in the  ecosystem of stored maize.","palabras_clave":"Entomopathogenic fungi|Maize agroecosystem|Stored pest|LT50|Mortality"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64300,"idioma_id":4,"titulo":"Low Computational Burden Grid Voltage Sensorless Current Controller","resumen":"This paper proposes a grid voltage sensorless ROGI  based current controller. The proposal takes advantage of the  controller structure to avoid the grid voltage measurement,  avoiding the need of additional states and requiring only a few  multiply and add operations for its computation. This allows  to keep the controller?s computational burden to a minimum,  making it ideal for low cost DSP implementation.","palabras_clave":"Current controller|DPGS|ROGI|sensorless|VSC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64301,"idioma_id":4,"titulo":"Promoter sequence of Shiga toxin 2 (Stx2) is recognized in vivo, leading to production of biologically active Stx2","resumen":"ABSTRACT Shiga toxins (Stx) are the main agent responsible for the development of hemolytic-uremic syndrome (HUS), the most  severe and life-threatening systemic complication of infection with enterohemorrhagic Escherichia coli (EHEC) strains. We previously  described Stx2 expression by eukaryotic cells after they were transfected in vitro with the stx2 gene cloned into a prokaryotic  plasmid (pStx2). The aim of this study was to evaluate whether mammalian cells were also able to express Stx2 in vivo after  pStx2 injection. Mice were inoculated by hydrodynamics-based transfection (HBT) with pStx2. We studied the survival, percentage  of polymorphonuclear leukocytes in plasma, plasma urea levels, and histology of the kidneys and the brains of mice. Mice  displayed a lethal dose-related response to pStx2. Stx2 mRNA was recovered from the liver, and Stx2 cytotoxic activity was observed  in plasma of mice injected with pStx2. Stx2 was detected by immunofluorescence in the brains of mice inoculated with  pStx2, and markers of central nervous system (CNS) damage were observed, including increased expression of glial fibrillary  acidic protein (GFAP) and fragmentation of NeuN in neurons. Moreover, anti-Stx2B-immunized mice were protected against  pStx2 inoculation. Our results show that Stx2 is expressed in vivo from the wild stx2 gene, reproducing pathogenic damage induced  by purified Stx2 or secondary to EHEC infection.","palabras_clave":"HUS|PAGHE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64302,"idioma_id":4,"titulo":"Gerstmann-Sträussler-Scheinker syndrome with variable phenotype in a new kindred with PRNP-P102L mutation","resumen":"Gerstmann-Sträussler-Scheinker syndrome (GSS) is a dominantly inherited disorder belonging to the group of transmissible human spongiform encephalopathies or prion diseases. Several families affected by GSS with patients carrying mutations in the prion protein gene have been described worldwide. We report clinical, genealogical, neuropathology and molecular study results from two members of the first Argentine kindred affected by GSS. Both family members presented a frontotemporal-like syndrome, one with and the other without ataxia, with different lesions on neuropathology. A Pro to Leu point mutation at codon 102 (P102L) of the prion protein gene was detected in one of the subjects studied. The pathogenic basis of phenotypic variability observed in this family remains unclear, but resembles that observed in other P102L GSS patients from the same family.","palabras_clave":"Argentina|ataxia|Gerstmann-Sträussler-Scheinker|P102L|variable phenotype"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64303,"idioma_id":4,"titulo":"The VLT LBG Redshift Survey - III. The clustering and dynamics of Lyman-break galaxies at z ˜ 3","resumen":"We present a catalogue of 2135 galaxy redshifts from the VLT LBG Redshift Survey (VLRS), a spectroscopic survey of z &#8776; 3 galaxies in wide fields centred on background quasi-stellar objects. We have used deep optical imaging to select galaxies via the Lyman-break technique. Spectroscopy of the Lyman-break galaxies (LBGs) was then made using the Very Large Telescope (VLT) Visible Multi-Object Spectrograph (VIMOS) instrument, giving a mean redshift of z = 2.79. We analyse the clustering properties of the VLRS sample and also of the VLRS sample combined with the smaller area Keck-based survey of Steidel et al. From the semiprojected correlation function, wp(&#963;), for the VLRS and combined surveys, we find that the results are well fit with a single power-law model, with clustering scale lengths of r0 = 3.46 ± 0.41 and 3.83 ± 0.24 h-1 Mpc, respectively. We note that the corresponding combined &#958;(r) slope is flatter than for local galaxies at &#947; = 1.5-1.6 rather than &#947; = 1.8. This flat slope is confirmed by the z-space correlation function, &#958;(s), and in the range 10 < s < 100 h-1 Mpc the VLRS shows an &#8776;2.5&#963; excess over the &#923; cold dark matter (&#923;CDM) linear prediction. This excess may be consistent with recent evidence for non-Gaussianity in clustering results at z &#8776; 1. We then analyse the LBG z-space distortions using the 2D correlation function, &#958;(&#963;, &#960;), finding for the combined sample a large-scale infall parameter of &#946; = 0.38 ± 0.19 and a velocity dispersion of sqrt{< w_z^2rangle }=420^{+140}_{-160} km s^{-1}. Based on our measured &#946;, we are able to determine the gravitational growth rate, finding a value of f(z = 3) = 0.99 ± 0.50 (or f&#963;8 = 0.26 ± 0.13), which is the highest redshift measurement of the growth rate via galaxy clustering and is consistent with &#923;CDM. Finally, we constrain the mean halo mass for the LBG population, finding that the VLRS and combined sample suggest mean halo masses of log(MDM\/M&#8857;) = 11.57 ± 0.15 and 11.73 ± 0.07, respectively.","palabras_clave":"galaxies: kinematics and dynamics|cosmology: observations|large-scale structure of Universe"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64304,"idioma_id":4,"titulo":"Effect of bars in AGN host galaxies and black hole activity","resumen":"Aims: With the aim of assessing the effects of bars on active  galactic nuclei (AGN), we present an analysis of host characteristics  and nuclear activity of active galaxies with and without bars.   Methods: We selected AGN host galaxies from the Sloan Digital Sky Survey  Data Release 7 (SDSS-DR7), using the available emission-line fluxes. By  visual inspection of SDSS images we classified the face-on spiral hosts  of AGNs brighter than g-mag &lt; 16.5 into barred or unbarred. With the  purpose of providing an appropriate quantification of the effects of  bars, we also constructed a suitable control sample of unbarred active  galaxies with similar redshift, magnitude, morphology, bulge sizes, and  local environment distributions.  Results: We find that the bar  fraction, with respect to the full sample of spiral face-on AGN host  galaxies, is 28.5%, in good agreement with previous works. barred AGN  host galaxies show an excess of young stellar populations (as derived  with the Dn(4000) spectral index), dominated by red u - r and  g - r colors, with respect to the control sample, suggesting that bars  produce an important effect on galaxy properties of AGN hosts. Regarding  the nuclear activity distribution, we find that barred active galaxies  show a shift toward higher Lum [OIII] values with respect to AGN without  bars. In addition, we also find that this trend is more significant in  less massive, younger stellar population and bluer AGN host galaxies. We  found that the fraction of powerful AGN increases toward more massive  hosts with bluer colors and younger stellar populations residing in  denser environments. However, barred host AGN systematically show a  higher fraction of powerful active nuclei galaxies with respect to the  control sample. We also explored the accretion rate onto the central  black holes, finding that barred AGN host show an excess of objects with  high accretion rate values with respect to unbarred active gala","palabras_clave":"GALAXIES|ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64305,"idioma_id":5,"titulo":"Características bio-ópticas y morfometría de una laguna de zona templada","resumen":"El objetivo de este trabajo fue evaluar la correlación entre sensores de campo e imágenes satelitales en la caracterización bio-óptica de la laguna Chasicó (Argentina) en el período febrero-abril de 2010. Los parámetros morfométricos del cuerpo de agua se obtuvieron a partir de mediciones in situ y datos satelitales. Los sensores empleados permitieron la adquisición de firmas espectrales en las que se detectaron algunas características del agua superficial: turbidez, clorofila-a y variaciones de profundidad. Como resultado del análisis morfométrico, se obtuvo que la laguna superó los 50 km2 de extensión y presentó una forma subrectangular elongada. A partir del procesamiento de imágenes satelitales, datos de clorofila-a y el cálculo del Índice de Vegetación de Diferencia Normalizada (en inglés, NDVI), se modeló la distribución espacial del pigmento en la laguna. El 53,2 % de su área presentó entre 5 y 10 &#956;g\/l de clorofila-a, por lo que fue caracterizada como un lago mesotrófico para el período estudiado.","palabras_clave":"LAGUNA CHASICO|BIO-OPTICA|CLORFILA|GEOMORFOLOGIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64306,"idioma_id":5,"titulo":"Experiencias paranormales y su relación con el sentido de la vida","resumen":"asa","palabras_clave":"parapsicologia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64307,"idioma_id":4,"titulo":"Bis(oxalato)dioxovanadate(V) and bis(oxalato)oxoperoxo- vanadate(V) complexes: Spectroscopic characterization and biological activity","resumen":"Abstract Two structurally related vanadium(V) complexes,K3[VO2(C2O4)2]·3H2O and K3[VO(O2)(C2O4)2]·1\/2H2O,were thoroughly characterized by infrared, Raman, and electronicspectroscopies. The effect of both complexes on theviability of the human MG-63 osteosarcoma cells was testedusing the MTTassay. The monoperoxo complex shows a verystrong antiproliferative activity (at 100-&#956;Mconcentration, thiscomplex diminished the cell viability ca. 80 %), whereas thedioxo complex was inactive.","palabras_clave":"Vanadium(V)|Bis(oxalato) complexes|Peroxo ligand|Vibrational spectra|Electronic spectra|Osteosarcoma cells in culture"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64308,"idioma_id":4,"titulo":"Spectroscopic Characterization of an Oxovanadium(IV) Complex of Oxodiacetic Acid and 2,2'-Bipyridine. Bioactivity on Osteoblast-Like Cells in Culture","resumen":"                      The  oxovanadium(IV) complex of oxodiacetic acid (H2ODA) and  2,2?-bipyridine (bipy) of stoichiometry [VO(ODA)(bipy)]×H2O, was  thoroughly characterized by infrared, Raman and electronic spectroscopies. The  biological activity of the complex on the cell proliferation was tested on  osteoblast-like cells (MC3T3E1 osteoblastic mouse calvaria-derived cells and  UMR106 rat osteosarcoma-derived cells) in culture. The complex caused  inhibition of cellular proliferation in both osteoblast-like cells in culture, but  its action was statistically stronger in the tumoral cells. This effect was  specially marked with increasing concentrations of the complex. Based on these  preliminary biological results, [VO(ODA)(bipy)]×H2O can  be considered as a good candidate to be further investigated in relation to  cancer treatment.   ","palabras_clave":"Oxovanadium(IV)|Oxodiacetic acid|2,2'-Bipyridine|Vibrational spectra|Electronic spectra|Osteoblast cells in culture|Antitumoral action"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64309,"idioma_id":5,"titulo":"Respuesta de células hipofisarias al estrés oxidativo producido por riboflavina en terapia fotodinámica","resumen":"Hipótesis: La riboflavina, debido al buen rendimiento cuántico de O2 (1&#61508;g)  podría utilizarse como fotosensibilizador (FS) en terapia fotodinámica (TFD). Objetivos: Analizar la respuesta de células epiteliales a la TFD mediada por riboflavina (RF), estudiando la ultraestructura de las mismas. Identificar las especies reactivas de oxígeno (ERO) involucradas en este mecanismo y determinar el tipo de muerte celular. Materiales y Métodos: Para mejorar la penetración a través de la membrana plasmática, se acetiló la RF obteniendo RF liposoluble (RFL). Con la finalidad de constatar la generación de ERO por ambas RF a longitud de onda (&#955;) de 460 nm se utilizaron 3-hidroxipiridina y 9-10 dimetilantraceno, drogas que se descomponen en presencia de O2 (1&#8710;g). Se verificó el daño celular generado por la acción de ambos FS, RF y RFL activados por luz en células dispersas de tejido hipofisario de ratas. Para comprobar las ERO involucradas se inhibió la citotoxicidad utilizando antioxidantes: catalasa (75 U\/ml), superóxido dismutasa (100 U\/ml) y L-Histidina (10 mM). Se midió la viabilidad celular con azul de tripán y se analizó la respuesta por microscopía electrónica de transmisión y microscopía fotónica de alta resolución. Resultados: Se demostró que ambas RF iluminadas con el prototipo de lámpara  de 10 mW\/cm2 y 460 nm de &#955;, generan ERO que conducen a la muerte celular por dos mecanismos morfológicamente bien diferenciados, necrosis y apoptosis. Conclusión: La citotoxicidad generada por la activación de ambas RF, nos permite sugerir su utilización como FS en la aplicación de TFD.","palabras_clave":"riboflavina|terapia fotodinámica|estrés oxidativo |muerte celular"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64310,"idioma_id":4,"titulo":"Bottom-up and top-down COBOL system migration to Web Services: An experience report (Indexed SCI, IF JCR2012=2.039)","resumen":"Moving from mainframe systems to a service-oriented architecture (SOA) using Web services is an attractive but daunting task. The bottom-up or direct-migration approach enables the effective modernization of legacy systems to Web services. Conversely, bringing migration into fruition with the top-down or indirect-migration approach is more difficult, but it achieves better migration results. Employing both approaches on the same large enterprise system is uncommon, which leaves no room for comparison. This article describes the migration processes, costs, and outcomes of applying both approaches on a real Cobol system.","palabras_clave":"Web Services Modeling|Services Architectures"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64311,"idioma_id":5,"titulo":"¿Qué sujeto de aprendizaje? Recuperar el pasado para pensar la intervención psicopedagógica","resumen":"El presente trabajo se desprende del análisis documental realizado en el marco de un Seminario de Maestría en Educación que permitió acceder a información sobre el origen de las prácticas Psicopedagógicas. Si bien la asignatura \u201CPsicopedagogía\u201D integraba los planes de estudio de la carrera de magisterio en la década de los 50, -tal vez por la influencia de las ideas de Víctor Mercante-, la primera intervención específica la constituyen \u201CLas misiones Psicopedagógicas\u201D que especialistas de la Universidad Nacional del Sur realizaron &nbsp;en toda la región, destinada a brindar asesoramiento y orientación psicopedagógica a profesores&nbsp; y relevar el nivel socio-económico y mental de la población. Otro hecho significativo lo constituye la creación en el año 1960 del \u201CInstituto de Capacitación Docente para Escuelas Hogares\u201D cuyo plan de estudios de dos años tenía como principales materias: Psicometría, Psicopedagogía, Pedagogía Diferenciada, Psicología clínica entre otras. Dependiendo de este Instituto se crean en 1962 los \u201CGabinetes Psico Médico Pedagógico\u201D para docentes de las Escuelas Hogares, a los efectos de realizar estudios, diagnósticos y recomendar tratamientos que demandaran dichas escuelas. Nos preguntamos ¿Qué dificultades entrañaba la enseñanza en estas instituciones que requería de estos dispositivos? ¿Qué características particulares poseían estos grupos culturales que requerían de estudios específicos y de profesionales especializados en virtud de que no eran permeables a la acción de la escuela? Estar advertidos de estos orígenes que \u2013con otros escenarios y ropajes diferentes-, pueden hacernos perder el sentido de nuestra intervención.","palabras_clave":"PSICOPEDAGOGIA|SUJETO DE APRENDIZAJE|EDUCACION|PROVINCIA DE RIO NEGRO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64312,"idioma_id":4,"titulo":"GEOLOGICAL EVIDENCE FOR A DROUGHT EPISODE IN THE WESTERN PAMPAS (ARGENTINA, SOUTH AMERICA) DURING THE EARLY- MID 20TH CENTURY","resumen":"Drought episodes during the early-mid 20th century were recognized and described in several places around the world, with extreme dry conditions and widespread landscape denudation, like during the famous ¨Dust Bowl¨ in North America. However, there is scant documentation of droughts in southern South America, particularly from the Pampas, and none based on the geological record. In this article we provide clear evidence of aeolian reactivation and sand 0deposition in some areas of La Pampa and San Luis provinces, western Pampas (Argentina), during early-mid 20th century in response to drier conditions, probably amplified, like historic droughts in North America, by anthropogenic factors (e.g. significant population increase and agriculture expansion into a fragile environment). Evidence includes widespread bare sand blowouts, extensive surfaces with active sand migration, steep dune lee slopes and sharp crests covered by weak soil development (A\/C profile), accompanied by historical documents. OSL ages on aeolian beds confirm mobilization and sedimentation by wind processes ca. 95-60 yr BP. Considering the dominant (over 70%) austral spring-summer precipitation it is possible the rainfall deficit in western Pampas was linked to positive SST anomalies in the western subtropical South Atlantic Ocean (20º-30º S and 30º-50º W), according to significant canonical correlation between the precipitation field in subtropical South America and the Atlantic Ocean SST anomalies.","palabras_clave":"aeolian record|OSL dating|Pampas dust bowl|20th century|western Pampas|South America"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64313,"idioma_id":5,"titulo":"'Incidencia de políticas de empleo y transferencias de ingreso sobre la desigualdad económica: La Argentina del post neoliberalismo (2003-2010)'","resumen":"En un contexto nacional e internacional en el cual el Estado se reinstala en un lugar protagónico de la esfera social, el trabajo se interroga acerca de la capacidad de los programas de empleo y transferencias de ingreso para alterar el patrón de distribución de los ingresos en la Argentina durante el período post neoliberal.    Para desarrollar este análisis se aplica un ejercicio de descomposición del coeficiente de desigualdad de Gini. Se utilizan las bases de la Encuesta Permanente de Hogares (EPH) del Instituto Nacional de Estadísticas y Censos (INDEC), correspondientes a la Región del Gran Buenos Aires.   La evidencia empírica presentada dará cuenta de que los procesos que explicaron en mayor medida la distribución más progresiva de los ingresos entre los años 2003 y 2010 están asociados a las políticas sociales y de intervención estatal.","palabras_clave":"DESIGUALDAD |TRANSFERENCIAS DE INGRESO|PROGRAMAS DE EMPLEO|DESCOMPOSICIÓN DEL COEFICIENTE DE GINI"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64314,"idioma_id":4,"titulo":"Therapeutic improvement of a stroma-targeted CRAd by incorporating motives responsive to melanoma microenvironment","resumen":"We have previously designed a conditionally replicative oncolytic adenovirus (CRAd) named Ad-F512 that can target both the stromal and the malignant melanoma cell compartments. The replication capacity of this CRAd is driven by a 0.5&#8201;Kb SPARC promoter fragment (named F512). To improve CRAd´s efficacy we cloned into F512, motives responsive to hypoxia (HRE) and inflammation (NF-&#954;B) to obtain a chimeric promoter named &#954;BF512HRE. Using luciferase as a reporter gene we observed 10?15 fold increased activity under hypoxia and 10-80-fold induction upon TNF-&#945; addition. We next constructed a CRAd (Ad-&#954;BF512HRE) where E1A activity was under &#954;BF512HRE regulation. Treatment of nude mice harboring established tumors made of a mix of SB2 melanoma cells and WI-38 fibroblasts with Ad-&#954;BF512HRE led to the complete elimination of tumors in 100% of mice (8\/8). Moreover, Ad-5\/3-&#954;BF512HRE, a viral variant pseudotyped with a chimeric 5\/3 fiber exerted a strong lytic effect on CAR-negative melanoma cells and was highly effective in vivo on established tumors made of melanoma cells and WI-38 fibroblasts leading to the complete elimination of 4\/5 tumors. These results indicate that this improved stroma-targeted oncolytic adenovirus can override the resistance of melanoma tumors and might become of significant importance for melanoma therapeutics.","palabras_clave":"ADENOVIRUS ONCOLITICOS|MELANOMA|CANCER|SPARC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64315,"idioma_id":5,"titulo":"LA ACTUACIÓN DEL MINISTERIO PÚBLICO FISCAL EN PROCESOS AMBIENTALES NO PENALES: DEFENSA DEL ORDEN PÚBLICO AMBIENTAL O INTERVENCIÓN MERAMENTE DECORATIVA?","resumen":"El trabajo aborda el alcance del precedente e incidencia refleja de los criterios  hermenéuticos aplicados por el TSJ en la fijación de los límites de la actuación del MPF en los procesos ambientales que se tramiten en sede civil. La inexistencia de juzgados con competencias especiales en la materia, implica que buena parte de las causas ambientales no penales, se tramiten en el fuero civil. Algunas de ellas, se encuentran actualmente a resolución del mismo Tribunal, habiendo ejercido el MPF conforme su unidad orgánica, el impulso de la acción de oficio \u2013atento el carácter indisponible del proceso-, mediante una actuación recursiva en cada instancia procesal .En ese marco, el propósito del artículo es realizar algunas inferencias acerca de la aplicabilidad de los parámetros hermenéuticos fijados por el Tribunal, para la actuación del MPF en los procesos ambientales que se tramiten en juzgados civiles y si el mismo tiene facultades impugnativas  para recurrir las resoluciones que afectan los intereses colectivos que protege.La legitimación del Ministerio Público para accionar el proceso de protección de los derechos de incidencia colectiva, es derivación directa de las normas constitucionales que lo habilitan para promover e intervenir en los procesos en que se hayan podido producir lesiones de los derechos difusos o de incidencia colectiva. El criterio restrictivo introducido por la regla del Tribunal Superior de Justicia de la provincia de Córdoba, por la que \u201Cen materia civil el Ministerio Público Fiscal carece de legitimación recursiva autónoma\u201D, no resulta de aplicación analógica ni extensiva a los procesos colectivos ambientales, se trate de amparo ambiental colectivo -art. 43 CN- o acciones declarativas de inconstitucionalidad, u otros procesos especiales y autónomos como las acciones de cese, prevención y recomposición de daño ambiental previstas en la Ley General del Ambiente, que tramiten en el fuero civil.El Ministerio Público Fiscal no es un mero participante que ejerce las funciones de contralor del proceso, actuando por vía de dictámenes, pero sin tener legitimación para recurrir. Por el contrario, resulta parte obligada y debe necesariamente dársele intervención recursiva en los procesos colectivos ambientales. Si la propia Constitución califica como esencial y legítima la calidad de parte del Ministerio Público, mal pueden fundarse criterios hermenéuticos que aniquilen su legitimación y capacidad de actuación, en un preexistente reconocimiento de normas adjetivas de rango inferior.","palabras_clave":"MINISTERIO PÚBLICO FISCAL|LEGITIMACIÓN |PROCESO AMBIENTAL|ORDEN PÚBLICO AMBIENTAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64316,"idioma_id":4,"titulo":"The Origin and Construction of Pre-Hispanic Mounds in the Upper Delta of the Paraná River (Argentina)","resumen":"Pre-Hispanic mounds, known as \u201Ccerritos,\u201D \u201Ccerri-tos de indios,\u201D or \u201Caterros\u201D across southeastern South Amer-ica, are one of the most conspicuous and well-studied culturalmanifestations in lowlands archaeology. Nevertheless, in theUpper Delta of the Paraná River, mounds are rarely studied,and even their anthropic origin is under debate. This could berelated to the fact that anthropogenic mounds are located on a floodplain where other \u201Cmound-like\u201D natural geoforms(generated by fluvial processes) are also present. In additionto this, the natural geoforms also contain evidence of Holo-cene human occupation (sherds, bones, charcoal, humans burials, etc.), which can lead to interpretive errors of their origin and formation. Thus, this project set out to determinethe genesis and evolution of these moundsand also toidentifythe cultural occupation and transformation of natural land-forms found in the area. In this article, natural and anthropo-genic systems and processes wereidentifiedand characterizedthrough the application of proxy record analysis (i.e., sedi-ment composition, stratigraphy, micromorphology, silica bod-ies and chronological analysis) at the Los Tres Cerros archaeological locality in the Upper Delta of the Paraná River of Victoria County, Entre Ríos Province, Argentina. This anal-ysis allowed forthe recognitionofnatural anthropogenic inter-faces, such as the \u201Cpre-mound\u201D occupation as well as evidenceof cultural activities such as mound construction, between1,000 and 500 14C years BP. These findings were integratedinto current research on the variability of mound constructionduring the Late Holocene in the lowlands of South America","palabras_clave":"MOUNDS|'CERRITOS'|UPPER DELTA OF THE PARANA RIVER|HOLOCENE|DEPOSITIONAL SYSTEMS|WETLANDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64317,"idioma_id":4,"titulo":"Palaeo-environmental reconstruction of La Olla, a Holocene archaeological site in the Pampean coast (Argentina).","resumen":"The evolution of littoral palaeoenvironments in the southern Pampas region of Argentina towards the end of the early Holocene and the beginning of the mid Holocene are discussed, and the formation  processes of the archaeological site of La Olla are analyzed. This site is located in the intertidal zone of the present beach and is remarkably well-preserved.The human occupation at the site has been dated at  between 7400 and 6480 yrs BP, and is interpreted as a place for the processing and consumption of the southern fur seal (Arctocephalus australis) and southern sea-lion (Otaria flavescens). It is concluded  that biostabilization by microbial mats of the sedimentary structures and archaeological remains that developed and are present on the tidal flat prevented their erosion by wind, turbulence currents and waves. This is what has permitted the exceptional preservation of the site. On the basis of the analyses of one of the sectors of the site it can be concluded that the sedimentary succession of the La Olla site is the result of the coastal environmental changes during the early-mid Holocene in the pre-maximum transgressive episode. During this interval a tidal flat developed on the Pleistocene abrasion platform.  Human occupation occurred during the formation of mixohaline marsh, an upper intertidal-lower supratidal zone ecosystem","palabras_clave":"COASTAL HUNTER-GATHERERS|MICROBIAL MATS|COASTAL ADAPTATION|SITE FORMATION PROCESSES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64318,"idioma_id":4,"titulo":"Radiocarbon Chronology of the Early Human Occupation of Argentina","resumen":"Se presentan y discuten las dataciones radiocarbónicas de Argentina  proveniente de sitios arqueológicos en el lapso 7.000 - 13.000 años AP. Se analizan a la luz de criterios de aceptación y se evalúan estadísticamente. Se ponen a prueba los modelos de poblamiento con las dataciones mas antiguas de Argentina.","palabras_clave":"RADIOCARBON CHRONOLOGY|ARGENTINA|PEOPLING OF THE AMERICAS|LATE PLEISTOCENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64319,"idioma_id":4,"titulo":"Optimization of the hydrolysis of lignocellulosic residues by using radial basis functions modeling and particle swarm optimization","resumen":"The concentrations of glucose and total reducing sugars obtained by chemical hydrolysis of three different lignocellulosic feedstocks were maximized. Two response surface methodologies were applied to model the amount of sugars produced: (1) classical quadratic least-squares fit (QLS), and (2) artificial neural networks based on radial basis functions (RBF). The results obtained by applying RBF were more reliable and better statistical parameters were obtained. Depending on the type of biomass, different results wereobtained. Improvements in fit between 35% and 55% were obtained when comparing the coefficients of determination (R2) computed for both QLS and RBF methods. Coupling the obtained RBF models with particle swarm optimization to calculate the global desirability function, allowed to perform multiple response optimization. The predicted optimal conditions were confirmed by carrying out independent experiments.","palabras_clave":"GLUCOSE|MODELING|OPTIMIZATION|ARTIFICIAL INTELLIGENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64320,"idioma_id":4,"titulo":"Sucrose signaling in plants A world yet to be explored","resumen":"      The role of sucrose as a signaling  molecule in plants was originally proposed several decades ago. However,  recognition of sucrose as a true signal has been largely debated and only recently  this role has been fully accepted. The best-studied cases of sucrose signaling  involve metabolic processes, such as the induction of fructan or anthocyanin  synthesis, but a large volume of scattered information suggests that sucrose signals  may control a vast array of developmental processes along the whole life cycle  of the plant. Also, wide gaps exist in our current understanding of the  intracellular steps that mediate sucrose action. Sucrose concentration in plant  tissues tends to be directly related to light intensity, and inversely related  to temperature, and accordingly, exogenous sucrose supply often mimics the  effect of high light and cold. However, many exceptions to this rule seem to  occur due to interactions with other signaling pathways. In conclusion, the  sucrose role as a signal molecule in plants is starting to be unveiled and much  research is still needed to have a complete map of its significance in plant function.  ","palabras_clave":"SUCROSE SIGNALING|LOW TEMPERATURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64321,"idioma_id":4,"titulo":"Water State and Carbonyl Distribution Populations in Confined Regions of Lipid Bilayers Observed by FTIR Spectroscopy","resumen":"It has been suggested that water in confined regions presents different properties than bulk water, mainly due to the changes in water population species that may be induced by the adjacent walls of different polarities, in terms of hydrogen bonding formation.   In this context, it would be expected that lipids in the gel and the fluid state should offer different templates for water organization. The presence of water pockets or defects in lipid bilayers has been proposed to explain the insertion of charged and polar peptides and aminoacids in membranes. In this work we provide direct evidence by means of FTIR spectroscopy that water band profiles are changed whether lipids are either in the solid state, in the gel state after heating and cooling across the phase transition or in the fluid state. The different bands found in each case were assigned to different H bonded water populations in correspondence to the exposure of carbonyl groups.","palabras_clave":"WATER|CARBONYL GROUPS|REGIONS|FTIR SPECTROSCOPY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64322,"idioma_id":4,"titulo":"Connected and isolated CH2 populations in acyl chains and its relation to pockets of confined water in lipid membranes as observed by FTIR spectrometry","resumen":"Analysis of the band corresponding to the frequency of vibrational symmetric stretching mode of methylene  groups in the lipid acyl chains and the bands of water below and above the phase transition of different lipids  by Fourier transform infrared spectroscopy gives strong support to the formation of confined water pockets  in between the lipid acyl chains. Our measures and analysis consolidate the mechanism early proposed by  Traüble, in the sense that water is present in kinks formed by trans-gauche isomers along the hydrocarbon  tails. The formation of these regions depends on the acyl lipid composition, which determines the presence  of different populations of water species, characterized by its degree of H bond coordination in fluid saturated  or unsaturated lipids. The free energy excess due to the reinforcement of the water structure along few water  molecules in the adjacencies of exposed membrane residues near the phase transition is a reasonable base to  explain the insertion and translocation of polar peptides and aminoacid residues through the biomembrane  on thermodynamic and structural grounds.","palabras_clave":"CH2 POPULATIONS|SYMMETRIC STRETCHING MODE|MEMBRANE|ENERGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64323,"idioma_id":4,"titulo":"Evaluation of the Defay-Prigogine model for the membrane interphase in relation to biological response in membrane-protein interactions","resumen":"Surface water activity appears as a common factor when the interaction of several aqueous soluble and surface active proteins with lipid membranes of different compositions is measured by the changes in surface pressure of a lipid monolayer. The perturbation of the lipid surface caused by aqueous soluble proteins depends on the composition of the hydrocarbon phases, either modified by unsaturated bonds in the acyl chains or by inclusion of cholesterol. The cut-off (critical) surface pressure in monolayers, at which no effect of the proteins is found, is related to the composition of the head group region. The perturbation of surface pressure is produced by proteins when the area per lipid is above just 4% larger than that corresponding to the hydration shell of the phospholipid head groups found in the cut-off. This area excess gives place to regions in which the chemical potential of water changes with respect to bulk water. According to the Defay?Prigogine relation this interfacial water activity is the reason of the surface pressure increase induced by aqueous soluble proteins injected in the subphase. As predicted by solution chemistry, the increase of surface pressure is independent of the protein nature but depends on the water surface state determined by the lipid composition.","palabras_clave":"Lipid membranes|Watermembrane-interphases|Monolayer surface pressure|Interfacial water activity|Protein interaction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64324,"idioma_id":4,"titulo":"BIOLOGICAL ACTIVITY OF ANTIBACTERIAL PEPTIDES MATCHES SYNERGISM BETWEEN ELECTROSTATIC AND NON ELECTROSTATIC FORCES","resumen":"available online: 30-OCT-2013  Substitution of Ala 108 and Ala 111 in the 107-115 human lysozyme (hLz) fragment results in a 20-fold increased anti-staphylococcal activity while its hemolytic activity becomes significant (30%) at very high concentrations. This analog displays an additional positive charge near the Nterminus (108) and an extra Trp residue at the center of the molecule (111), indicating that this particular amino acid sequence improves its interaction with the bacterial plasma membrane. In order to understand the role of this arrangement in the membrane interaction, studies with model lipid membranes were carried out.The interactions of peptides, 107-115 hLz and the novel analog ([K108W111]107-115 hLz) with liposomes and lipid monolayers were evaluated by monitoring the changes in the fluorescence of the Trp residues and the variation of the monolayers surface pressure, respectively. Results obtained with both techniques revealed a significant affinity increase of [K108W111]107-115 hLz for lipids, especially when the membranes containing negatively charged lipids, such as phosphatidylglycerol. However, there is also a significant interaction with zwitterionic lipids, suggesting that other forces in addition to electrostatic interactions are involved in the binding.The analysis of adsorption isotherms and the insertion kinetics suggest that relaxation processes of the membrane structure are involved in the insertion process of novel peptide [K108W111]107-115 hLz but not in 107-115 hLz, probably by imposing a reorganization of water at theinterphases. In this regard, the enhanced activity of peptide [K108W111]107-115 hLz may be explained by a synergistic effect between the increased electrostatic forces as well as the increased hydrophobic interactions.","palabras_clave":"HEMOLYTIC ACTIVITY|ELECTROSTATIC INTERACTIONS|RELAXATION|PEPTIDE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64325,"idioma_id":5,"titulo":"Tributos e inclusión social","resumen":"  Este trabajo tiene como objetivo indagar sobre aspectos impositivos en el sistema tributario argentino, específicamente el Régimen Simplificado para Pequeños Contribuyentes que ha sido estructurado de manera tal que representen una respuesta inmediata al reclamo político-social de igualdad, inclusión social y distribución del ingreso.","palabras_clave":"IMPUESTOS|FINANZAS|MONOTRIBUTO|SOCIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64326,"idioma_id":5,"titulo":"Estado, sociedad y medios de comunicación Contribuciones para pensar la política","resumen":"El trabajo tiene el propósito de indagar, en la Teoría Social Latinoamericana, la relación Estado, Sociedad y Medios de comunicación. Aunque esta intención expresa un carácter eminentemente teórico se sustenta en la intrincada e ineludible relación entre teoría y realidad social. Relación que lleva a preguntarnos por la concreción de las prácticas políticas hoy vigentes. Esto implica re-pensar, no solamente los modelos de Estado en pugna, que van desde la intervención pro-cíclica y distributiva a los que lo definen como mero reproductor de la explotación y la desigualdad, sino también por las distintas concepciones de individuo-sociedad que trasvasan dichos modelos y el rol que dichos modelos le asignan a los medios de comunicación.Se trata, entonces, de rescatar los aportes que se construyeron desde los enfoques del Desarrollismo y la Dependencia, por entenderlos válidos y pertinentes para analizar la realidad actual de nuestro continente. Ciertamente, muchos de los problemas estructurales actuales de las sociedades capitalistas latinoamericanas se pueden pensar a la luz de esos aportes que comenzaron a construirse en la década de los ´60. La noción de desarrollo, con distintas variantes, no sólo es una constante en el pensamiento latinoamericano sino que atraviesa la política contemporánea.","palabras_clave":"TEORIA SOCIAL|ESTADO|SOCIEDAD|MEDIOS DE COMUNICACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64327,"idioma_id":4,"titulo":"Structural glance into a novel anti-staphylococcal peptide","resumen":"Novel antimicrobial peptides are valuable molecules for developing anti-infective drugs to counteract the contemporary spread of microbial drug-resistance. Here we focus on a novel peptide (RKWVWWRNR-NH2) derived from the fragment 107-115 of the human lysozyme that displays a 20-fold increase in anti-staphylococcal activity. The conformational analysis of this peptide and its interaction with model lipidic phases ?as assayed by circular dichroism and fluorescence spectroscopy?show a noteworthy spectral change, which might be related to its anti-staphylococcal activity. The secondary structure of peptide [K108W111] 107-115 hLz was dramatically affected through a single substitution at position 111 (Ala by Trp). Therefore this conformational change might improve the interaction of the novel peptide with the bacterial plasma membrane. These results highlight the role of peptide secondary structure and the distribution of polar\/non-polar residues for the effective interaction of this peptide with the bacterial plasma membrane, a key step for triggering its lethal effect. This knowledge may contribute to the rational design of a new generation of antimicrobial peptides with increased efficacy developed from natural sources by simple screening tools.","palabras_clave":"HUMAN LYSOZYME|ANTIMICROBIAL PEPTIDES|CIRCULA DICHROISM|FLUORESCENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64328,"idioma_id":4,"titulo":"Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria","resumen":"The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed.We present here the construction and functional validation of a new set of miniTn5 promoter traps to generatetnpR-based RIVET libraries. The ability to generate tnpRtranscriptional fusions by transposition will signi&#64257;cantlyfacilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.","palabras_clave":"MiniTn5|RIVET|tnpR|promotor probe"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64329,"idioma_id":4,"titulo":"Policies for the development of digital terrestrial television in Argentina","resumen":"Following the ups-and-downs of the selection of the digital TV standard, in the second half of 2009 Argentina decided to take the same path as Brazil, and adopted the Japanese-Brazilian standard. Since then concrete policies and actions have been introduced to implement Digital Terrestrial Television (DTT). Three years into the process, it is possible to put this into perspective and analyse the virtues and deficiencies of DTT deployment in Argentina. This article is intended to focus on the public policies introduced by the Argentine State both in the deployment of the necessary infrastructure and in the stimulus to content production. What is special about the Argentine case is the leading role of the State.","palabras_clave":"TDT|Argentina|Media policy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64330,"idioma_id":5,"titulo":"'El Framework CIRA, un aporte a las técnicas de File Carving'","resumen":"File Carving es el proceso de extraer archivos de un medio de almacenamiento en ausencia de metadatos del sistema de archivo, es decir, directamente a partir del análisis del contenido de los bloques de disco. Pese a la existencia de varias técnicas y algoritmos de file carving, no hay definido aún un proceso que sea aplicable a todos. En este trabajo se presenta una solución de file carving ? CIRA ?, su implementación, su Framework, su arquitectura y algunos resultados obtenidos.","palabras_clave":"CIRA|FILE|CARVING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64331,"idioma_id":5,"titulo":"Transformaciones y fragilidades ambientales en la pampa argentina","resumen":"La historia agroproductiva ambiental pampeana se adscribe a la explotación de los recursos naturales que han articulado los ciclos de producción dominantes desde fines del siglo XIX. La acción productiva sobre los territorios rurales pampeanos introdujo efectos ambientales que trastocaron la dinámica de los agroecosistemas de la región. Con el incremento de la demanda que profundiza la homogeneización productiva, se despliegan técnicas cada vez más agresivas originando efectos ambientales en el manejo del suelo, la contaminación por agroquímicos y la deforestación.  En un contexto de creciente competitividad que continúa sostenida en las ventajas comparativas y en la artificialización de sistemas de producción capitalizados, el abordaje de las relaciones sociedad\/naturaleza a través de estudios de historia ambiental, se posiciona como un elemento clave en la agenda de investigación para comprender el devenir de la región pampeana argentina.","palabras_clave":"trasnformaciones|ambiente|rural|pampa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64332,"idioma_id":4,"titulo":"EBV primary infection in childhood and its relation to B-cell lymphoma development: a mini-review from a developing region","resumen":"In most underdeveloped countries the initial contact with Epstein Barr virus (EBV) usually happens in the first decade of life and results in an asymptomatic infection, while in developed areas primary infection in adolescence or adulthood is accompanied by infectious mononucleosis in 50% cases.  Although it is generally a harmless passenger, in some individuals, it is associated with B-cell lymphoma. In Argentina, EBV primary infection shows the classical pattern observed in developing populations, given that nearly 70% of patients are seropositive by the age of 2 years. However, EBV association with paediatric Hodgkin and Burkitt lymphoma resembles that observed in developed regions. Concerning diffuse large B-cell lymphoma our series demonstrated higher EBV association than other adult ones from either developed or underdeveloped countries. Interestingly, the early EBV primary infection observed, characteristic of an underdeveloped population, together with the statistically significant EBV association with patients <10 years old demonstrated in all types of lymphoma studied, suggest a relationship between low age of EBV seroconversion and B-cell lymphoma development risk.","palabras_clave":"Epstein Barr|Hodgkin lymphoma|B-cell Non Hodgkin lymphoma|childhood"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64333,"idioma_id":4,"titulo":"Simulations of photocurrent improvement through combined geometric\/diffracting light trapping in organic small molecule solar cells","resumen":"Although organic solar cells have recently shown remarkable high power   conversion efficiencies approaching 12%, further improvements are needed   to become a low cost alternative to current inorganic photovoltaic   technologies. Optical losses due to insufficient light trapping,   parasitic absorption in the contact layers and reflectance limit   drastically the photocurrent delivered by these solar cells. In this   work, we simulated two- (2D) and three-dimensional (3D) surface textures   in the micro- and submicroscale to improve light trapping in optimized   organic solar cells based on copper phtalocyanine (CuPc) and fullerene   (C60). The analysis was carried out with the aid of the   finite element method in 2D and 3D, taking into account interference as   well as reflection and diffraction of the incident AM1.5 spectrum. At   normal incidence, up to 23% improvement in the photocurrent over the   planar cell was obtained. To investigate the texture performance under   practical circumstances, we simulated 2D microstructures during a   typical summer day, taking the change of incidence angle and radiation   intensity into account. Results clearly show that all textured cells   deliver more photocurrent than the planar cell, even at oblique angles.","palabras_clave":"FINITE ELEMENTS METHOD|LIGHT TRAPPING|ORGANIC SOLAR CELLS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64334,"idioma_id":4,"titulo":"Change Operations and Their Consequences in AOP Evolution","resumen":"Simple change operations to source code can cause unexpected behavior. In particular, two well-known problematic situations, fragile pointcuts and aspect interactions, pose serious challenges for the evolution of software with aspects. We believe that if developers are informed about the consequences of the change operations they are considering, they will be able to avoid various errors. This work analyzes the effects of change operations in AO applications. It proposes the Identification, Qualitative, and Quantity (IQQ) model as a conceptual approach to anticipating the consequences of change operations, along with BaLaLu, a tool that supports the IQQ model.","palabras_clave":"Aspect-oriented Programming|Separation of Concerns|Change Operations|Software Evolution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64335,"idioma_id":5,"titulo":"Directrices para la construcción de un repositorio temático","resumen":"La creación de repositorios de información institucionales y\/o temáticos, en nuestros días es la clave para el acceso a producción científica de manera libre y abierta.Por lo tanto, el acceso a la información a través de repositorios de información se ve favorecido por las iniciativas que propone el Acceso Abierto (AA).Para la creación de un repositorio de información en el marco de la filosofía de AA es importante contar con directrices, éstas pretenden ser una guía general de aspectos básicos dónde se detallan los pasos necesarios para la creación de un repositorio.El presente trabajo recoge las directrices para la construcción de repositorios temáticos a partir de los siguientes puntos de referencia: finalidad, funciones, políticas y actividades.Aunque existe un consenso sobre que los repositorios temáticos fueron los primeros en aparecer, existe poca información sobre desarrollo y gestión de repositorios temáticos comparado con la amplitud de información alcanzada por repositorios institucionales","palabras_clave":"repositorios temáticos|directrices|acceso abierto|thematic repositories"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64336,"idioma_id":4,"titulo":"Phage Biocontrol of Enteropathogenic and Shiga Toxin-Producing Escherichia coli during milk fermentation","resumen":"Two bacteriophages, isolated from feces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5&#945;, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10 C, the strain used as lactic starter, reached 109 cfu ml-1 after 4 h, while pH values fell to 4.5 after 8 h, regardless of the presence of E. coli strains and\/or phages. In absence of phages, E. coli strains reached 4-6 log cfu ml-1 at 5-6 h. E. coli DH5&#945; and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (&lt;10 cfu ml-1) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log cfu ml-1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.","palabras_clave":"ESCHERICHIA COLI O157:H7 STEC|BACTERIOPHAGE|PHAGE BIOCONTROL|MILK FERMENTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64337,"idioma_id":4,"titulo":"Osteology and phylogenetic relationships of Tyrannotitan chubutensis Novas, de Valais, Vickers- Rich and Rich, 2005 (Theropoda: Carcharodontosauridae) from the Lower Cretaceous of Patagonia, Argentina.","resumen":"The theropod clade Carcharodontosauridae is a broadly distributed group of large allosauroid theropods. The carcharodontosaurids recorded in the Albian-Cenomanian of Gondwana are the youngest and most derived members of this clade. Tyrannotitan chubutensis, from the Cerro Castaño Member of Cerro Barcino Formation (Albian; Chubut Group), Central Patagonia, Argentina is of prime interest among Gondwanan carcharodontosaurids as it represents the oldest record of the group. Here we offer a detailed osteological comparative description of the holotype and paratype of Tyrannotitan chubutensis together with a new diagnosis of the species. The new information results in a better understanding of this taxon and Carcharodontosauridae. Furthermore, a comparative study of the anatomy of the pectoral girdle of Giganotosaurus is reinterpreted as very similar to that of Tyrannotitan and Mapusaurus. We also present a phylogenetic analysis of Carcharodontosauridae that recovers Tyrannotitan as a derived carcharodontosaurid, being the sister group of the clade formed by Giganotosaurus and Mapusaurus, all nested in the clade Giganotosaurini.","palabras_clave":"Theropoda|Cretaceous|Carcharodontosauridae|Phylogeny"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64338,"idioma_id":4,"titulo":"Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 158C storage","resumen":"To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum(20C). In this work, Micro-Tom tomatoes stored at 20C (normal ripening) were compared with those stored at 15Cor 4C(chilling injury inducer) for 7 days. In contrast to 4C, storage at 15C delayed ripening with the benefit of not enhancingoxidative metabolism and of enabling ripening upon being transferred to 20C. The transcriptional expression profile ofenzymes related to cell wall metabolism was compared at the three temperatures. Although endo-b-1,4-glucanase (Cel1),which is associated with fruit decay, was largely increased after removal from 4Cstorage, its expression was not modified infruits stored at 15C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase\/hydrolases (XTHs)XTH1, ?2,?10 and ?11, and of two b-xylosidases (Xyl1?2) was detected in fruits stored at 15Cwith respect to those at 20C. Following2 days at 20C, these transcripts remained higher in fruits stored at 15Cand XHT3 and ?9 also increased. Ethylene evolutionwas similar in fruits kept at 15C and 20C; thus, the changes in the transcript profile and fruit properties between thesetreatments may be under the control of factors other than ethylene.","palabras_clave":"CELL WALL METABOLISM|CHILLING INJURY|COLD-STORAGE|ENDOTRANSGYLCOSYLASE\/HYDROLASE|RIPENING| XYLOGLUCAN &#914;-XYLOSIDASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64339,"idioma_id":4,"titulo":"Circadian oscillation and development-dependent expression of Glycine Rich RNA Binding Proteins in tomato fruits","resumen":"Glycine-rich RNA-binding proteins (GRPs) are involved in the modulation of the posttranscriptional processing oftranscripts and participate as an output signal of the circadian clock. However,neither GRPs nor the circadian rhythmic have been studied in detail in fleshy fruits yet. In the present work, GRP1 gene familywas analyzed in Micro-Tom tomato fruit. Three highly homologous LeGRP1 genes (LeGRP1a-c) wereidentified. For each gene, three products were found, corresponding to theun-spliced pre-RNA, the mature mRNA and the alternatively spliced mRNA (preLegrp1a-c, mLegrp1a-c and asLegrp1a-c,respectively). LeGRPs show the classical RNArecognition motif and glycine-rich region; and werefound in the nucleus and in the cytosol of tomato fruit. By using different Escherichia coli mutants, it was found that LeGRP1s harbored in vivo RNA-melting abilities and were able to complement thecold-sensitive phenotype of BX04 cells. Particularcircadian profiles of expression, dependent on the fruit developmental stage,were found for each Legrp1 form.During ripening off the vine of fruits harvested at the mature green stage, thelevels of all Legrp1a-c forms drasticallyincreased; however, incubation at 4ºC prevented such increases. Analysisof the expression of all Legrp1a-c formssuggests a positive regulation of expression in tomato fruit. Overall, the results obtained in this work reveal a complex pattern ofexpression of GRPs in tomato fruit, suggesting they might be involved in posttranscriptionalmodulation of circadian processes of this fleshy fruit.&nbsp;","palabras_clave":"tomato fruit|circadian clock|Glicine-rich protein|alternative spliced products"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64340,"idioma_id":5,"titulo":"Método Estadístico-Evolutivo para la Reducción de Incertidumbre en Procesos de Predicción","resumen":"Existen diversos factores que dificultan y limitan el modelado y la implementación de fenómenosambientales, y que impactan en la calidad de la predicción arrojada por la evaluación del modelo en función de los parámetros de entrada. Por su parte, existen diferentes métodos para la reducción de incertidumbre, los cuales permiten reducir los efectos negativos provocados por los aspectos no representables o cuantificables ya sea matemática o computacionalmente. En este trabajo se describe un método de reducción de incertidumbre basado en la estadística, la computación evolutiva y el paralelismo, denominado Evolutionary-Statistical System o ESS. El método es iterativo y dirigido por datos con solución múltiple solapada. Su potencia se basa en aumentar la predicción realizada por el modelo implementado en el simulador con la orientación de la búsqueda y la calibración de los&nbsp; resultados.","palabras_clave":"ESTADÍSTICA|INCERTIDUMBRE|ALGORITMOS EVOLUTIVOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64341,"idioma_id":4,"titulo":"Hybrid hard- and soft- modeling of spectrophotometric data for monitoring of ciprofloxacin and its main photodegradation products at different pH values","resumen":"A simple and fast on line spectrophotometric method combined with a hybrid hard-soft modelling multivariate curve resolution (HS-MCR) was proposed for the monitoring of photodegradation reaction of ciprofloxacin under UV radiation. The studied conditions attempt to emulate the effect of sunlight on these antibiotics that could be eventually present in the environment. The continuous flow system made it possible to study the ciprofloxacin degradation at different pH values almost at real time, avoiding errors that could arise from typical batch monitoring of the reaction. On the base of a concentration profiles obtained by previous pure soft-modelling approach, reaction pathways have been proposed for the parent compound and its photoproducts at different pH values. These kinetic models were used as a constraint in the HS-MCR analysis. The kinetic profiles and the corresponding pure response profile (UV-Vis spectra) of ciprofloxacin and its main degradation products were recovered after the application of HS-MCR analysis to the spectra recorded throughout the reaction. The observed behavior showed a good agreement with the photodegradation studies reported in the bibliography. Accordingly, the photodegradation reaction was studied by high performance liquid chromatography coupled with UV-Vis diode array detector (HPLC-DAD). The spectra recorded during the chromatographic analysis present a good correlation with the ones recovered by UV-Vis\/HS-MCR method.","palabras_clave":"MCR-ALS|KINETCS|CIPROFLOXACIN|PHOTODEGRADATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64342,"idioma_id":4,"titulo":"Total synchronous fluorescence spectroscopic data modeled with first- and second-order algorithms for the determination of doxorubicin in human plasma","resumen":"In this work, we present the development of a method for the determination of doxorubicin in plasma samples in the presence of an unexpected component (riboflavin) by using total synchronous fluorescence spectroscopic data matrices. To the best of our knowledge, this is the first time that the second-order advantage is obtained with this kind of  data. Two strategies including unfolding the data and: (a) processing with multivariate curve resolution coupled to alternating least-squares as first-order data or (b) processing with unfolded partial least-squares and exploiting the second order advantage by the residual bilinearization procedure were considered. The calibration set was built with human plasma samples spiked with doxorubicin, while the validation set was  prepared with human plasma samples spiked with both doxorubicin and riboflavin, a drug whose spectrum highly overlaps with the one corresponding to doxorubicin. Both methodologies reached good indicators of accuracy: recoveries of ca. 100 ± 8 % and REP of ca. 5 %; and precision: coefficient of variations between 7 and 9 %.","palabras_clave":"TOTAL SYNCHRONOUS FLUORESCENCE SPECTROSCOPY|MCR-ALS|U-PLS\/RBL|DOXORUBICIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64343,"idioma_id":4,"titulo":"Photoinduced electron transfer fluorometric Hg(II) chemosensor based on a BODIPY armed with a tetrapod receptor","resumen":"From the great variety of BODIPY based-chemosensors able to determine Hg2+, only a small portion has been applied to its determination in environmental and\/or biological samples. The lack of studies on  the analytical performance of the latter sensors makes interesting the development of investigations oriented to their possible analytical applications. The synthesis of a BODIPY derivative armed with a  tetrapod receptor is described. The procedure is based on a previous publication, and the modifications performed to improve the synthesis include alternative procedures with different objectives, as the  consecution of a multigram synthesis, improving the low yields of some of the previously proposed procedure steps, simplifying the experimental steps, achieving the desired purity requirements for use with analytical purposes, and enriching the characterization of the implied structures. The characteristics of its selectivity towards Hg2+ have been investigated, and the OFF-ON fluorometric response, based on a photo-electron transfer (PET) mechanism, served as the base for the development of a method able to determine Hg2+ in environmental waters at ng mL-1 levels.  The intrinsic fluorescence of the BODIPY core is inhibited and the probe exhibits a weak fluorescence (i.e. 'OFF' state due to the deactivating PET effect). Upon complexation, Hg2+ interacts with the lone-pair  electrons on the nitrogen atoms of the receptor moiety so that the electronic transfer from the receptor to the photo-excited fluorophore is slowed down or switched off (i.e. 'ON' state due to the suppression  of the deactivating PET effect by coordination of the analyte to the probe).  Regarding the complex photostability in aqueous solution, it is mandatory to conduct the experiments at darkness due to its photodegradation. The stoichiometry studies indicated a 1:2 relationship for the BODIPY-Hg2+ complex. The high selectivity towards mercuric ions is considerably influenced by pH, being necessary to conduct the experiments in a pH value higher than 6. Calibration samples were prepared by adding appropriate amounts of Hg2+ between 20.0-120.0 ng mL-1, at a constant BODIPY concentration of 1 mmol L-1. After agitating for 5 min at darkness, phosphate buffer (pH 7.50) was added, and it was diluted to the mark with water. Fluorescence measurements were carried out at 18 C,  exciting at 515 nm, and obtaining fluorescence emission at 538 nm. The method has been satisfactory applied to Hg2+ determination in environmental water samples.","palabras_clave":"BODIPY DERIVATIVE|MERCURY CHEMOSENSOR|FLUORIMETRIC ASSAY|AQUEOUS SOLUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64344,"idioma_id":5,"titulo":"Interferencia del Herpes virus equino 1 (EHV-1) en la apoptosis inducida","resumen":"La apoptosis es un tipo de muerte celular programada que puede ser desencadenada por múltiples factores, tanto internos como externos; dentro de estos últimos se encuentran las infecciones virales. Algunos alphaherpesvirus han desarrollado diversas estrategias para retardar o inhibir la muerte celular obteniendo, de esta manera, su propio beneficio al poder permanecer durante más tiempo en la célula. Hasta el momento no se ha identificado ningún mecanismo relacionado con la modulación de la muerte celular durante la infección con Herpesvirus equino tipo 1 (EHV-1). El objetivo del presente trabajo fue describir el efecto producido por la infección con EHV-1 sobre cultivos celulares inducidos a la muerte por apoptosis. La evaluación de la apoptosis se realizó mediante el reconocimiento de la fragmentación en escalera del ADN, la evaluación de la relación Anexina V\/ioduro de propidio (IP) y la determinación del clivaje de la citoqueratina 18, utilizando técnicas de inmunofluorescencia. Los resultados indican una posible interferencia del EHV-1 con la muerte por apoptosis hacia la mitad de su ciclo de replicación, que se incrementa hacia el final del mismo.","palabras_clave":"ANEXINA|APOPTOSIS|CITOQUERATINA 18|FRAGMENTACIÓN DEL ADN|HERPESVIRUS EQUINO 1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64345,"idioma_id":4,"titulo":"Accidental poisoning with Wedelia glauca ('sunchillo') in a bull confirmed by analysis of rumen content","resumen":"Wedelia glauca is an invasive, perennial plant of the Asteraceae family native to South America. Its toxicity is attributed to the presence of a hepatotoxic terpenoid known as atractyloside, a powerful inhibitor of cellular respiration and ATP synthesis. Cattle are the most frequently poisoned species, and the course of this poisoning is hyperacute or acute. Occasionally, it is possible to find fragments of plants in the rumen contents and indentify the dermis structure of the plants, as they do not undergo significant changes in spite of the mechanic and enzymatic activities occurring in the rumen. The macroscopic and microscopic anatomopathologic findings of a natural Wedelia glauca poisoning case in a Hereford bull are reported. It was confirmed by micrographic analysis of plant fragments found in the rumen contents and also in bales used to feed those animals.","palabras_clave":"bull|poisoning |Wedelia glauca|micrographic analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64347,"idioma_id":4,"titulo":"Advanced Glycation Endproducts and Alendronate differentially inhibit early and late osteoclastogenesis in vitro","resumen":"Advanced Glycation Endproducts (AGEs) are greatly elevated in bone extracellular matrix of patients withDiabetes mellitus, and this has been associated with the increased incidence of fractures observed in these patients.AGEs affect the homeostasis of bone cells such as osteoblasts and osteoclasts. Bisphosphonates are first-lineanti-osteoporotic drugs that principally exert their effects by inhibiting osteoclastic activity. However, the effect ofbisphosphonate treatment on bone quality in patients with Diabetes is uncertain. In the present work we haveevaluated the action of AGEs (50-200 &#956;g\/ml), with or without Alendronate (10-8-10-4M), on osteoclastogenesisinduced by co-cultures of Raw 264.7 macrophages and UMR106 osteoblasts. We determined the effects of differentculture conditions on osteoclastic recruitment, tartrate-resistant acid phosphatase (TRAP) activity and expression ofRAGE (receptor for AGEs); and on the osteoblastic expression of RANK ligand (RANKL). AGEs and Alendronateinhibited the recruitment and TRAP activity of osteoclasts, with an additive effect of both agents at high concentrationsof Alendronate. While AGEs prevented the early and late stages of osteoclastogenesis, Alendronate (alone or inco-incubation with AGEs) only inhibited its later stages. In addition, both AGEs and Alendronate increased theosteoclastic expression of RAGE and decreased the osteoblastic expression of RANKL, correlating closely withtheir inhibition of osteoclastogenesis. If these in vitro results can be extrapolated to a clinical setting, they may beindicating a potentiation of the anti-resorptive effects of Alendronate in the context of bone extracellular matrix withexcess accumulation of AGEs, as might occur in a patient with Diabetes mellitus.","palabras_clave":"Advanced glycation endproducts|Osteoclasts|Alendronate|RAGE|RANKL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64348,"idioma_id":4,"titulo":"Post-weaning cranial ontogeny in two bandicoots (Mammalia, Peramelomorphia, Peramelidae) and comparison with carnivorous marsupials","resumen":"The ontogeny of the skull has been studied in several marsupial groups such as didelphids, microbiotheriids, and dasyurids. Here, we describe and compare the post-weaning ontogeny of the skull in two species of bandicoots, Echymipera kalubu (Echymiperinae) and Isoodon macrourus (Peramelinae), analyzing specific allometric trends in both groups, describing common (and specific) patterns, and discussing them on functional and phylogenetic grounds. Growth patterns were analyzed both qualitatively and quantitatively, including bivariate and multivariate analyses of allometry. We also evaluated character transformation and phylogenetic signals of the allometric patterns in several groups of marsupials and some placentals. We identified morphological changes between juvenile and adult stages in bothspecies of peramelids, many related to the development of the trophic apparatus. Notable differences were detected in the patterns of growth, suggesting divergences in ontogenetic trajectories between both species. Both bivariate and multivariate methods indicate that positive allometries in E. kalubu apply to longitudinal dimensions, whereas in I. macrourus, positive allometries are restricted to vertical dimensions of the skull. The comparison of the allometric trends of two bandicoots with previously studied taxa reveals that although peramelids exhibit a particularly short gestation period and divergent morphology compared to other marsupials, their pattern does not show any particular trend. Some allometric trendsseem to be highly conserved among the species studied, showing weak phylogenetic signal. Marsupials in general do not show particular patterns of post-weaning skull growth compared with placentals.","palabras_clave":"Australidelphians |Marsupials |Post-weaning ontogeny |Skull morphology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64349,"idioma_id":4,"titulo":"Pleistocene Extinctions and the Perceived Morphofunctional Structure of the Neotropical Felid Ensemble","resumen":"The felid Neotropical ensemble has experienced important changes from the Pleistocene to the present, the extinction of sabertooth cats being the most significant. Assuming that the morphofunctional structure of the Neotropical felid ensemble was maximally expressed when machairodontines were present, we added specimens of Smilodon fatalis and S. populator to a morphological dataset inclusive of all extant species of Neotropical felids to explore the hypothetical effect of extinction of machairodontines on the perceived morphofunctional structure of the ensemble. We studied 321 specimens of 12 extant species of Neotropical felids plus S. populator and S. fatalis by measuring 123 skull and postcranial morphofunctional variables. We used Principal Component Analysis to find morphofunctional patterns of the skull, postcranium, and both combined (with and without correction for size) of both ensembles, past (with Smilodon) and present (with Smilodon lost to extinction). Canonical Phylogenetic Ordination was performed to assess the degree of phylogenetic influence on this morphospace. We also explored the effect of including homotherines in the Pleistocene guild by including specimens of North American Homotherium serum. Size was the principal factor structuring ensembles. Important morphological characters were associated to ecological performance of species. A pattern of bodily proportions was apparent: more stout species with larger skull, longer rostrum, stronger bite force, and longer pectoral crests, versus gracile, jumping species with opposing characters. The felid morphospace shrank after the extinction of Smilodon and Homotherium, but the configuration of the portion of space containing extant felids remained stable. This pattern is associated with deep phylogenetic roots.","palabras_clave":"South America|Felidae|Neotropic|Pleistocene|Ecomorphology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64350,"idioma_id":4,"titulo":"Response to Comment on ?The Placental Mammal Ancestor and the Post?K-Pg Radiation Of Placentals","resumen":"Tree-building with diverse data maximizes explanatory power. Application   of molecular clock models to ancient speciation events                          risks a bias against detection of fast   radiations subsequent to the Cretaceous-Paleogene (K-Pg) event. Contrary   to Springer                          et al., post?K-Pg placental diversification does not require ?virus-like? substitution rates. Even constraining clade ages to their                          model, the explosive model best explains placental evolution.","palabras_clave":"placental origins"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64351,"idioma_id":4,"titulo":"Hydrophilic and hydrophobic interactions in cross-linked chitosan membranes","resumen":"Chitosan membranes with different cross-linking density were prepared by modifying cross-linkingtime. Sodium tripolyphosphate was the cross-linking agent. A pulsed nuclear magnetic resonancestudy was performed on uncross-linked and cross-linked membranes. Different fraction of water moleculeswere identified in different zones within the membranes. The ratio of water molecules per chitosanrepeating unit were calculated. A maximum of twelve water molecules were tightly coordinated tothe chitosan repeating unit. Also, a very small water molecule fraction was identified but it was mobileenough as not to contribute to the dipolar interactions. The cross-linking reaction could lead to theformation of hydrophilic and hydrophobic interactions. These two types of interactions could result inthe coexistence of a network formed by hydrophilic and hydrophobic micropores. This knowledge couldbe useful for the interpretation of results of hydrophobic drugs permeation across hydrophilic membranes.For example, the increment of estradiol fluxes across chitosan membranes with an increase incross-linking density.","palabras_clave":"BIOMATERIALS|POLYMERS|NMR|DIFFUSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64352,"idioma_id":4,"titulo":"De novo assembly and characterization of leaf transcriptome for the development of functional molecular markers of the extremophile multipurpose tree species Prosopis alba","resumen":"BackgroundProsopis alba (Fabaceae) is an important native tree adapted to arid and semiarid regions of north-western Argentina which is of great value as multipurpose species. Despite its importance, the genomic resources currently available for the entire Prosopis genus are still limited. Here we describe the development of a leaf transcriptome and the identification of new molecular markers that could support functional genetic studies in natural and domesticated populations of this genus.ResultsNext generation DNA pyrosequencing technology applied to P. alba transcripts produced a total of 1,103,231 raw reads with an average length of 421 bp. De novo assembling generated a set of 15,814 isotigs and 71,101 non-assembled sequences (singletons) with an average of 991 bp and 288 bp respectively. A total of 39,000 unique singletons were identified after clustering natural and artificial duplicates from pyrosequencing reads.Regarding the non-redundant sequences or unigenes, 22,095 out of 54,814 were successfully annotated with Gene Ontology terms. Moreover, simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were searched, resulting in 5,992 and 6,236 markers, respectively, throughout the genome. For the validation of the the predicted SSR markers, a subset of 87 SSRs selected through functional annotation evidence was successfully amplified from six DNA samples of seedlings. From this analysis, 11 of these 87 SSRs were identified as polymorphic. Additionally, another set of 123 nuclear polymorphic SSRs were determined in silico, of which 50% have the probability of being effectively polymorphic.ConclusionsThis study generated a successful global analysis of the P. alba leaf transcriptome after bioinformatic and wet laboratory validations of RNA-Seq data.The limited set of molecular markers currently available will be significantly increased with the thousands of new markers that were identified in this study. This information will strongly contribute to genomics resources for P. alba functional analysis and genetics. Finally, it will also potentially contribute to the development of population-based genome studies in the genera.","palabras_clave":"Transcriptomics|Bioinformatics|Molecular markers|Prosopis alba"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64353,"idioma_id":4,"titulo":"Self-assembly of 2,3-dihydroxycholestane steroids into supramolecular organogels as a soft template for the in-situ generation of silicate nanomaterials","resumen":"Supramolecular gels are an important and interesting class of soft materials that show great potential for many applications. Most of  them have been discovered serendipitously, and understanding the supramolecular self-assembly that leads to the formation of the  gel superstructure is the key to the directed design of new organogels. We report herein the organogelating property of four  stereoisomers of the simple steroid 2,3-dihydroxycholestane. Only the isomer with the trans-diaxial hydroxy groups had the ability  to gelate a broad variety of liquids and, thus, to be a super-organogelator for hydrocarbons. The scope of solvent gelation was  analysed with regard to two solvent parameters, namely the Kamlet?Taft and the Hansen solubility parameters. The best correlation  was observed with the Hansen approach that revealed the existence of two clear gelation zones. We propose a general model of  self-assembly through multiple intermolecular hydrogen bonds between the 1,2-dihydroxy system, which is based on experimental  data and computational simulations revealing the importance of the di-axial orientation of the hydroxy groups for the one-dimensional  self-assembly. Under controlled conditions, the fibrillar superstructure of the organogel was successfully used as a template  for the in-situ sol?gel polymerization of tetraethoxysilane and the further preparation of silica nanotubes. We propose that the  driving forces for templating are hydrogen bonding and electrostatic interactions between the anionic silicate intermediate species  and the self-assembled fibrillar network.","palabras_clave":"Hansen parameters|nanotube|self-assembly|supramolecular gel"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64354,"idioma_id":4,"titulo":"MALT lymphoma in labial salivary gland biopsy from Sjögren","resumen":"Mucosa-associated lymphoid tissue (MALT) lymphomas are known to occur in Sjögren syndrome (SS) patients, butreported cases in labial salivary glands (LSG) are rare. We report a case of 60-year-old female patient with SS who developedMALT lymphoma in the labial salivary glands during a 2-year time interval when she was participating in the Sjögren\u2019sInternational Clinical Collaborative Alliance, an ongoing longitudinal multisite observational study funded by the NationalInstitutes of Health of the United States. At follow-up exam, LSG biopsy showed atypical diffuse infiltration by mononuclearcells of variable size and atypical nuclei affecting the whole specimen with destruction of glandular architecture, leading to adiagnosis of B-cell MALT lymphoma. Computerized tomography and bone marrow biopsy failed to show additional evidenceof disease. Clinical, serologic, ocular, histologic and immunohistochemical findings are presented. A \u201Cwatch and wait\u201D policywas adopted with regular examinations.","palabras_clave":"SJOGREN SYNDROME|SALIVARY GLAND|DIAGNOSIS|BIOPSY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64355,"idioma_id":4,"titulo":"Late Quaternary environmental changes in southernmost South America reflected in marine calcareous macro-and-microfossils","resumen":"Abstract  Late Quaternary environmental changes, especially sea-level fluctuations and glacial advances and retreats, affected the distribution of the benthic marine fauna which inhabited the Beagle Channel in southernmost South America. To evaluate these changes, calcareous macro-and microfossils obtained from Holocene marine sediments along a sector of the western side of the Beagle Channel have been used for a multi-proxy approach. In total, 119 species were recovered:65 mollusks (24 bivalves, 35 gastropods and 6 chitons), 33 foraminifera and 21 ostracods. Other  preserved calcareous groups, which appeared in minor proportions, were brachiopods and cirripeds. The calcareous fossil assemblages from the early Holocene represent an ecosystem transition which started with vacant niches first occupied by opportunistic species, and\/or  eurytopic taxa. These were then replaced by more diverse fauna assemblages, associated with the proliferation of habitats which occurred under truly marine conditions during the Mid Holocene.  When Holocene calcareous fauna is compared with its Pleistocene counterparts from the same region, some differences in the proportion of taxonomic groups and taxa composition are noted.  These partly follow the heterogeneity of habitats in the Beagle Channel and water masses of different origin which have either greater or less influence throughout the Late Quaternary, but  they can also be interpreted in association with environmental changes after the Last Glacial Maximum. In conclusion, the data provides insights into both spatial and temporal changes, and shows evidence of a gradual transformation from a brackish to a marine environment caused by  the Holocene sea transgression. It also documents Late Quaternary faunistic changes associated with topographic and oceanographic local conditions in this microbasin.","palabras_clave":"HOLOCENE|LATE PLEISTOCENE|TIERRA DEL FUEGO|MOLLUSKS|FORAMINIFERA|OSTACODS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64356,"idioma_id":4,"titulo":"Preyssler catalyst-promoted rapid, clean and efficient condensation reactions for 3H-1,5-benzodiazepine synthesis in solvent free conditions","resumen":"A simple, convenient, and environmentally benign synthesis of 1,5-benzodiazepines is developed bycondensing different o-phenylenediamines and 1,3-aryl-1,3-propanodiones. The reaction is catalyzedby a Preyssler (NaH14P5W30O110, HPA) heteropolyacid as a safe, clean, and recyclable catalyst. The methodis operationally simple and provides access to a variety of 1,5-benzodiazepines in good to excellentyields.","palabras_clave":"3H-1,5-Benzodiazepines|o-Phenylenediamine|1,3-Diaryl-1,3-propanediones|Preyssler catalyst"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64357,"idioma_id":5,"titulo":"Trayectorias personales e identidades profesionales. 'Ingenieros y Licenciados copan el nivel medio\u201C","resumen":"Este estudio de carácter preliminar, y desde el escenario inicial que brindan datos tratados estadísticamente, expresiones de los medios de comunicación y percepciones sociales que se manifiestan en la cotidianeidad de las instituciones educativas, se interesa por saber   quiénes son y qué trayectorias han desarrollado sujetos concretos, profesionales, que no tienen título de profesores y dan clases de matemática, biología, física y química en el nivel medio de la provincia de Río Negro. Adoptamos una perspectiva que se apoya en el entrecruzamiento de la historia de los sujetos concretos, la historia de la dinámica y las características del trabajo docente en el nivel medio y también del contexto social general, ya que si se construyeran esos elementos se advertiría la complejidad de la realidad socioeducativa y se comprenderían cómo, a pesar de ciertas homogeneidades estructurales, pueden reconocerse recorridos diferentes y también diferentes significaciones otorgadas a las mismas oportunidades disponibles (Cragnolino, 2006). Se llevaron a cabo entrevistas en profundidad a profesionales, maestros y profesores que enseñan ciencias en el nivel medio, centrando la atención para este avance preliminar en seis profesionales que trabajan en el nivel medio de Río Negro.","palabras_clave":"TRAYECTORIAS|IDENTIDAD PROFESIONAL|EDUCACION|NIVEL MEDIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64358,"idioma_id":4,"titulo":"Salicylic acid determines differential senescence produced by two Turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes","resumen":"Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus(TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae,including&nbsp; Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica junceaplants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway","palabras_clave":"TUMV|SENESCENCE|SALICYLIC ACID|ROX"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64359,"idioma_id":4,"titulo":"Transcriptional regulation of fatty acid biosynthesis in mycobacteria","resumen":"The main purpose of our study is to understand howmycobacteria exert control over the biosynthesis oftheir membrane lipids and find out the key componentsof the regulatory network that control fatty acidbiosynthesis at the transcriptional level. In this articlewe describe the identification and purification of FasR,a transcriptional regulator from Mycobacterium sp.that controls the expression of the fatty acid synthase(fas) and the 4-phosphopantetheinyl transferase(acpS) encoding genes, whose products are involvedin the fatty acid and mycolic acid biosynthesis pathways.In vitro studies demonstrated that fas and acpSgenes are part of the same transcriptional unit and thatFasR specifically binds to three conserved operatorsequences present in the fas-acpS promoter region(Pfas). The construction and further characterizationof a fasR conditional mutant confirmed that FasR is atranscriptional activator of the fas-acpS operon andthat this protein is essential for mycobacteria viability.Furthermore, the combined used of Pfas?lacZ fusionsin different fasR backgrounds and electrophoreticmobility shift assays experiments, strongly suggestedthat long-chain acyl-CoAs are the effector moleculesthat modulate the affinity of FasR for its DNA bindingsequences and therefore the expression of the essentialfas-acpS operon.","palabras_clave":"transcriptional regulatio|regulation lipid metabolism|mycobacterium|tuberculosis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64360,"idioma_id":4,"titulo":"ASCORBYL PALMITATE INTERACTION WITH PHOSPHOLIPID MONOLAYERS: ELECTROSTATIC AND RHEOLOGICAL PREPONDERANCY","resumen":"Ascorbyl palmitate (ASC16) is an anionic amphiphilic molecule of pharmacological interest due to its antioxidant properties. We found that ASC16 strongly interacted with model membranes. ASC16 penetrated phospholipid monolayers, with a cut off near the theoretical surface pressure limit. The presence of a lipid film at the interface favored ASC16 insertion compared with a bare air\/water surface. The adsorption and penetration time curves showed a biphasic behavior: the first rapid peak evidenced a fast adsorption of charged ASC16 molecules to the interface that promoted a lowering of surface pH, thus partially neutralizing and compacting the film. The second rise represented an approach to the equilibrium between the ASC16 molecules in the subphase and the surface monolayer, whose kinetics depended on the ionization state of the film. Based on the Langmuir DMPC+ASC16 monolayer data, we estimated an ASC16 partition coefficient to DMPC monolayers of 1.5x10  rheological properties of the host membrane were determinant for ASC16 penetration kinetics: a fluid membrane, as provided by Chol, disrupted the liquid-condensed ASC16-enriched domains and favored ASC16 penetration. Subphase pH conditions affected ASC16 aggregation in bulk: the smaller structures at acidic pHs showed a faster equilibrium with the surface film than large lamellar ones. Our results revealed ASC16 interaction with model membranes has a highly complex regulation. The polymorphism in the ASC16 bulk aggregation added complexity to the equilibrium between the surface and subphase form of ASC16, whose understanding may shed light on the pharmacological function of this drug.","palabras_clave":"GIBBS MONOLAYERS|BREWSTER ANGLE MICROSCOPY|LIQUID-CONDENSED DOMAINS|VITAMIN C DERIVATIVES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64361,"idioma_id":4,"titulo":"DOPAMINE INTERFERES  WITH APPETITIVE LONG-TERM MEMORY FORMATION IN HONEY BEES","resumen":"        Studies  in vertebrates and invertebrates have  proved the instructive role that different biogenic amines play in  the neural representation of rewards and punishments during  associative learning.  Results from diverse arthropods and using different learning paradigms  initially agreed that dopamine (DA) is needed for aversive learning and octopamine (OA) is needed for appetitive learning. However,the notion that both amines constitute separate pathways for  appetitive and aversive learning is changing. Here, we asked whether DA, so  far only involved in aversive memory formation in honey bees, does also modulate appetitive memory. Using the well characterized appetitive olfactory conditioning of the proboscis extension reflex (PER), we show that DA impairs appetitive memory consolidation. In addition, we found that blocking DA  receptors  enhances appetitive memory. These  results are consistent with the view that aversive and appetitive components interact during learning and memory formation to ensure adaptive behavior.  ","palabras_clave":"Aprendizaje|Memoria|Invertebrados|Dopamina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64362,"idioma_id":4,"titulo":"AlN thin films deposited by DC reactive magnetron sputtering: effect of oxygen on film growth","resumen":"Aluminum nitride is a ceramic compound with many technological applications in many fields, for example optics, electronics and resonators. Contaminants play a crucial role in the AlN performance. This paper focuses mainly in the effect of oxygen when AlN, with O impurities in its structure, is grown on oxidized layers. In this study, AlN thin films have been deposited at room temperature and low residual vacuum on SiO2\/Si (100) substrates. AlN films were grown by DC reactive magnetron sputtering (aluminum target) and atmosphere composed by an argon\/nitrogen mixture. Working pressure was 3 mTorr. Film characterization was performed by AES, XRD, SEM, EDS, FTIR, HRTEM, SAED and band-bending method. Our results show that oxidized interlayer imposes compressive stresses to AlN layer, developing a polycrystalline deposition. Indeed, when film thickness is over 900 nm, influence of oxidized interlayer diminishes and crystallographic orientation changes to the (0002) one, i.e., columnar structure, and stress relief is induced (there is a transition from compressive to tensile stress). Also, we propose a growth scenario to explain this behaviour.","palabras_clave":"SURFACE OXIDES|ALUMINUM NITRIDE|RESIDUAL STRESS|SPUTTERING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64363,"idioma_id":4,"titulo":"Regulatory and effector T-cells are  differentially modulated by Dexamethasone","resumen":"It is assumed that the ratio between effector T cells (Teff) and regulatory T cells (Tregs) controls the immune reactivity within the T-cell compartment. The purpose of this study was to investigate if Dexamethasone (Dex) affects Teff and Tregs subsets. Dex induced on Tregs a dose and time-dependent apoptosis which resulted in a relative increase of Teff. After TCR activation, Dex induced a strong proliferative inhibition of Teff, but a weaker proliferative inhibition on Tregs. These effects were modulated by IL-2, which not only restored the proliferative response, but also prevented Dex-induced apoptosis. The highest dose of IL-2 prevented apoptosis on all FOXP3+CD4+ T cells. Meanwhile, the lowest dose only rescued activated Tregs (aTregs), probably related to their CD25 higher expression. Because Dex did not affect the suppressor capacity of aTregs either, our results support the notion that under Dex treatment, the regulatory T-cell compartment maintains its homeostasis.© 2013.","palabras_clave":"TREGS|DEXAMETHASONE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64364,"idioma_id":5,"titulo":"Dissemination of blaIMP-8 among Enterobacteriaceae isolates from a Buenos Aires Hospital","resumen":"Entre agosto de 2008 y diciembre de 2011 se detectaron en el Hospital Interzonal General de Agudos «Evita» de Lanús 6 aislamientos de enterobacterias productoras de metalo-&#946;-lactamasas, distribuidos en tres especies: Enterobacter cloacae (4), Klebsiella oxytoca(1) y Citrobacter freundii (1). Los seis aislamientos presentaron un perfi l de multirresis-tencia y se confi rmó la presencia del gen blaIMP-8. Cinco aislamientos además expresaron la &#946;-lactamasa de espectro extendido PER-2. El gen blaIMP-8 fue hallado como primer case-te de un integrón de clase 1. Sin embargo, la secuencia 3 &#769; conservada no pudo detectarse en tres aislamientos. En todos los casos, el gen blaIMP-8 fue transferido por conjugación a Escherichia coli resistente a azida. Mediante PFGE se observó que los cuatro aislamientos de E. cloacae no estuvieron genéticamente relacionados. Estos son los primeros hallazgos de metalo-&#946;-lactamasas en la institución, que sugieren una posible diseminación horizon-tal del gen blaIMP-8 intra e interespecies.","palabras_clave":"CARBAPENEMASA|ENTEROBACTERIAS|Metalo- &#946; -lactamasas|BLAIMP-8"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64365,"idioma_id":4,"titulo":"Incipient loss of flagella in the genus Geolegnia: the emergence of a new clade within Leptolegnia?","resumen":"The genus Geolegnia represents a poorly documented group of saprolegnialean oomycetesisolated from soils as free-living organisms. Although it is morphologically similar to the facultativeparasitic genus Leptolegnia, Geolegnia presents the uncommon property of having lost a flagellateGeolegnia represents a poorly documented group of saprolegnialean oomycetesisolated from soils as free-living organisms. Although it is morphologically similar to the facultativeparasitic genus Leptolegnia, Geolegnia presents the uncommon property of having lost a flagellateLeptolegnia, Geolegnia presents the uncommon property of having lost a flagellatestage in its lifecycle. Based on ITS and large subunit (LSU) rRNA sequence data, we show GeolegniaGeolegniato be basal to Leptolegnia, and also introduce Geolegnia helicoides sp. nov. Using sequence data ofLeptolegnia, and also introduce Geolegnia helicoides sp. nov. Using sequence data ofLeptolegnia available in GenBank, supplemented by data derived from culture collections, we showthat Geolegnia is nested within Leptolegnia, a genus characterised by its ?conventional? biflagellateavailable in GenBank, supplemented by data derived from culture collections, we showthat Geolegnia is nested within Leptolegnia, a genus characterised by its ?conventional? biflagellateGeolegnia is nested within Leptolegnia, a genus characterised by its ?conventional? biflagellatelife cycle. The emergence of Geolegnia is therefore seen as a recent event, and we suggest here anevolutionary context where this loss might have been advantageous. Based on this study, LeptolegniaGeolegnia is therefore seen as a recent event, and we suggest here anevolutionary context where this loss might have been advantageous. Based on this study, LeptolegniaLeptolegniaremains paraphyletic, awaiting the redefinition of genera in this complex.","palabras_clave":"Geolegnia|Saprolegniales|Oomycetes|New species"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64366,"idioma_id":4,"titulo":"SPATIAL DISTRIBUTION OF PHLEBOTOMINAE IN PUERTO IGUAZÚ-MISIONES,  ARGENTINA-BRAZIL-PARAGUAY BORDER AREA","resumen":"The first Argentinean autochthonous human case of visceral leishmaniasis (VL) was confirmed in Posadas (Misiones) in 2006. Since then the disease has increased its incidence and geographical distribution. In the 2006- 2012 period, 107 human cases were detected (11deaths). The presence of Lutzomyia longipalpis was detected in peridomiciles in Puerto Iguazú urban area in 2010; some of these findings were associated with households where cases of canine VL had already been reported. The objective of this study was to ascertain the abundance and spatial distribution of Lu. longipalpis in Puerto Iguazú city, on the Argentina-Brazil-Paraguay border. Lu. longipalpis proved to be exclusively urban and was found in 31% of the households sampled (n=53), 67% of which belonged to areas of low abundance, 20% to areas of moderate abundance and 13% to areas of high abundance. Nyssomyia whitmani was the only species found both in urban and periurban environments, and Migonemyia migonei was registered only on the outskirts of the city.   Due to the fact that Puerto Iguazú is considered to be at moderate risk at the moment, it is necessary to intensify human and canine case controls, as well as take integrated prevention and control measures regarding the environment, vectors and reservoirs on the Argentina-Brazil-Paraguay border area.","palabras_clave":"PHLEBOTOMINAE FAUNA|LEISHMANIASIS|ARGENTINA-BRAZIL-PARAGUAY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64367,"idioma_id":4,"titulo":"Numerical Simulation of Finite Strain Viscoplastic Problems","resumen":"A large strain elastoplastic constitutive model based on hyperelasticity and multiplicative decomposition of deformation gradient tensor is extended to viscous case, in a framework similar to the one that has been proposed by Ponthot in an hypoelastic context. In this way a very useful framework can be obtained, able to deal with both rate dependent and rate independent problems.  In this work a review of theoretical details and numerical implementation of the model are discussed. Similarly to what is done in rate independent plasticity, a Newton\u2013Raphson scheme has been used to solve the non linear consistency condition in order to compute the viscoplastic multiplier.  A plane strain plate with a central circular hole under tension is simulated in order to test the proposed model. Large deformation effects are considered in all the simulations carried out. Different parameters of the constitutive model are varied in order to study the sensitivity of the proposed algorithm.","palabras_clave":"Viscoplasticity|Perzyna\u2019s model|Hyperelastic approach|Large strain|Finite elements"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64368,"idioma_id":4,"titulo":"Watching Every Step of the Way: Junín Virus Attenuation Markers in the Vaccine Lineage","resumen":"The Arenaviridae family includes several hemorrhagic fever viruses which are important emerging pathogens. Junín virus, a member of this family, is the etiological agent of Argentine Hemorrhagic Fever (AHF). A collaboration between the Governments of Argentina and the USA rendered the attenuated Junín virus vaccine strain Candid#1. Arenaviruses are enveloped viruses with genomes consisting of two single-stranded RNA species (L and S), each carrying two coding regions separated by a stably structured, non-coding intergenic region. Molecular characterization of the vaccine strain and of its more virulent ancestors, XJ13 (prototype) and XJ#44, allows a systematic approach for the discovery of key elements in virulence attenuation. We show comparisons of sequence information for the S RNA of the strains XJ13, XJ#44 and Candid#1 of Junín virus, along with other strains from the vaccine lineage and a set of Junín virus field strains collected at the AHF endemic area. Comparisons of nucleotide and amino acid sequences revealed different point mutations which might be linked to the attenuated phenotype. The majority of changes are consistent with a progressive attenuation of virulence between XJ13, XJ#44 and Candid#1. We propose that changes found in genomic regions with low natural variation frequencies are more likely to be associated with the virulence attenuation process. We partially sequenced field strains to analyze the genomic variability naturally occurring for Junín virus. This information, together with the sequence analysis of strains with intermediate virulence, will serve as a starting point to study the molecular bases for viral attenuation","palabras_clave":"Junin Virus|Vaccine|Virulence attenuation|molecular markers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64369,"idioma_id":4,"titulo":"Biocontrol of fungi isolated from sour rot infected table grapes by Saccharomyces and other yeast species","resumen":"Abstract: Sour rot is an important disease of grapes caused by an  etiologic complex of microorganisms in which filamentous fungi play a key role. Yeasts are used as biocontrol of pathogenic filamentous fungi in fruits. The major objective of the present work was to assess the in vivo effect of viticultural yeasts on phytopathogenic fungi involved in grape sour rot. Yeasts that were found to be effective in vivo against the fungi were assayed for their possible pathogenicity in humans: growth at 42 °C, pseudohyphal formation, adhesion, and phospholipase and protease activity. A total of 234 yeasts belonging to 14 genera were assayed against the following pathogens: Aspergillus caelatus, Aspergillus carbonarius, Aspergillus terreus, Aspergillus versicolor, Fusarium oxysporum, Penicillium comune, Rhizopus stolonifer and Ulocladium sp. Forty-three (16 Saccharomyces and 27 non-Saccharomyces) showed antagonistic properties against some of the fungi assayed in grapes at 25 °C. Yeast isolates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts (35), viticultural soils (6) and grape berries (2). Twenty biocontrol agents did not show phenotypical characteristics associated with pathogenicity in humans. This is the first study reporting on the antagonistic property of viticultural yeasts against 8 hytopathogenic fungi isolated from sour rot-damaged grapes. This fact makes our results even more relevant.","palabras_clave":"YEAST|BIOCONTROL|GRAPE|SOUR ROT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64370,"idioma_id":4,"titulo":"Synthesis and Characterization of a Zinc(II) Complex of Bispicen","resumen":"&nbsp;&nbsp;&nbsp;&nbsp; The synthesis of a Zn(II) complex of bispicen, thetetradentate ligand N,N\u2019-bis(2-pyridylmethyl)-ethylenediamine, of composition[Zn(bispicen)Cl(H2O)]2 (ZnCl4) is reported.Its crystal and molecular structure was determined by single- crystal X-raydiffraction methods. It crystallizes in the monoclinic space group C2\/c with Z = 4 molecules per unit cell. The Zn(II) cation in the[Zn(bispicen)Cl(H2O)]+ complex is in a distortedoctahedral environment, coordinated to a neutral bispicen molecule acting as atetra-dentate ligand through its two amine nitrogen atoms, at cis positions with each other, and itstwo pyridyl N-atoms, at transpositions. The six-fold coordination is completed by a chloride ion and a watermolecule. The tetrahedral [ZnCl4]2- counter-ion lies on acrystallographic two-fold axis. The complex was further characterized by FTIRspectroscopy, and its vibrational behavior compared with that of the dihydratedtetrahydrochloride of free bispicen.&nbsp; ","palabras_clave":"Zn(II)-bispicen complex|Crystal structure|FTIR-spectra"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64371,"idioma_id":4,"titulo":"Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate  insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases","resumen":"In the Upper Valley of Río Negro and Río Neuquén in Argentina, agriculture represents the second most important economic activity. Azinphos-methyl has been found in water from this region throughout the year at a maximum concentration of 22.48 µg L-1 during the application period. Toxicological studies on local non-target species have been performed mostly on vertebrates, while mollusks, which could be more sensitive, have not been studied so far. This work aims to characterize cholinesterase (ChE) and carboxilesterase (CE) activities of Chilina gibbosa, a freshwater gastropod native to southern Argentina and Chile. These enzymes, together with neurotoxicity signals, are evaluated herein after as sensitive biomarkers of exposure to azinphos-methyl at environmentally relevant concentrations. Effects of azinphos-methyl on antioxidant defenses: glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) are also studied in order to complete a set of biomarkers with different sensitivity and specificity, to propose C. gibbosa as a sentinel species. The highest specific activity was obtained with acetylthiocholine as substrate, followed by propionylthiocholine (83 % respect to acetylthiocholine ) and butyrylthiocholine (19 %).The lowest Km and the highest efficiency for ChE were obtained with acetylthiocholine. Regarding CEs activities, a higher efficiency was obtained with p-nitrophenyl butyrate than with p-nitrophenyl acetate. Eserine produced significant inhibition of ChE activity (81 % with 0.001 mM and 98 % with 1 mM) while iso-OMPA did not produce any significant effect on ChE. Our results show that C. gibbosa ChE is very sensitive to azinphos-methyl (CI50 0.02 µg L-1) while CEs are inhibited at higher concentrations (CI50 1000 µg L-1). CEs have been reported to be more sensitive to OPs than ChEs in most of the aquatic invertebrates protecting the organisms from neurotoxic effects. In contrast, C. gibbosa, has ChE which are much more sensitive to azinphos-methyl than CEs and shows marked signs of neurotoxicity. Regarding antioxidant defenses, GSH levels were significantly increased by 0.02 and 20 µg L-1 azinphos-methyl (80 and 103 %, respectively), CAT activity was increased 85% only at 0.02 µg L-1 and SOD and GST did not show any significant response. Since ChE activity, neurotoxicity signs, GSH and CAT are sensitive biomarkers of acute exposure to azinphos-methyl at environmental concentrations C. gibbosa could be included as sentinel species in monitoring programs of pesticide hazard in regions of Argentina and Chile.","palabras_clave":"AZINPHOS-METHYL|B-ESTERASES|INVERTEBRATES|ANTIOXIDANT DEFENSES|BIOMARKERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64372,"idioma_id":4,"titulo":"A new sphenodontian (Lepidosauria: Rhynchocephalia) from the Late Triassic of Argentina and the early origin of the herbivore opisthodontians","resumen":"Sphenodontians were a successful group of rhynchocephalian reptiles that dominated the fossil record of Lepidosauria during the Triassic and Jurassic. Although evidence of extinction is seen at the end of the Laurasian Early Cretaceous, they appeared to remain numerically abundant in South America until the end of the period. Most of the known Late Cretaceous record in South America is composed of opisthodontians, the herbivorous branch of Sphenodontia, whose oldest members were until recently reported to be from the Kimmeridgian?Tithonian (Late Jurassic). Here, we report a new sphenodontian, Sphenotitan leyesi gen. et sp. nov., collected from the Upper Triassic Quebrada del Barro Formation of northwestern Argentina. Phylogenetic analysis identifies Sphenotitan as a basal member of Opisthodontia, extending the known record of opisthodontians and the origin of herbivory in this group by 50 Myr.","palabras_clave":"LEPIDOSAURIA|SPHENODONTIA|OPISTHODONTIA|TRIASSIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64373,"idioma_id":4,"titulo":"Immune response and protection provided by live tachyzoites and native antigens from the NC-6 Argentina strain of Neospora caninum in pregnant heifers","resumen":"The aim of the present study was to compare the immunogenicity and protective efficacy of live tachyzoites and native antigen extract obtained from the NC-6 Argentina strain against vertical transmission of Neospora caninum, following experimental challenge in pregnant heifers with the NC-1 strain. Sixteen pregnant heifers were divided in 4 groups of 4 animals, each receiving different inoculation before mating: group A animals were intravenously (iv) inoculated with 6.25×10(7) live tachyzoites of the NC-6 strain, group B heifers were inoculated twice subcutaneously (sc) with N. caninum native antigen extract formulated with ISCOMs, group C heifers were sc injected with sterile phosphate-buffered saline (PBS) and group D heifers received sc ISCOM-matrix (ISCOMs without antigen). All groups were iv challenged with the NC-1 strain at 70 days of gestation. Serum and heparinized blood samples were collected eight times on weeks 0, 2, 3, 5, 9, 13, 16 and 17 post-inoculation. Dams were slaughtered at the 17th week of experiment (104 days of pregnancy) and placental and fetal tissue samples were collected. Specific antibody responses in heifers were tested by indirect enzyme linked immunosorbent assay (iELISA). The cellular immune response in dams was assessed by quantifying IFN-&#947; production and the percentages of T-cells (CD4(+), CD8(+) and &#947;&#948;(+)) and monocytes in peripheral blood mononuclear cells (PBMC). Fetal fluids and tissue samples were tested using the indirect fluorescence antibody test, western blot, histopathology, immunohistochemistry and nested-PCR. A significant increase in N. caninum antibody response was detected in heifers of groups A and B from week 3 after inoculation (P0.05). All fetuses were viable at necropsy. Specific IgG against N. caninum was detected in 1\/4 fetal fluids recovered from groups A, C and D heifers and 3\/4 fetal fluids from group B. Transplacental transmission could be determined in one fetus from group A and three fetuses from group B by nPCR. All fetuses from groups C and D were positive by nPCR. It is noteworthy that dams with higher CD4(+)\/CD8(+) ratios in PBMC, regardless of the experimental group, had lower pathology scores. The results of this study confirm that inoculation with live parasites pre-mating may provide at least partial protection against vertical transmission of N. caninum following challenge in heifers at early gestation.","palabras_clave":"Bovine|ISCOMs| Immunogens| Neospora caninum"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64374,"idioma_id":4,"titulo":"Manipulating the bioactivity of hydroxyapatite nano-rods structured networks: Effects on mineral coating morphology and growth kinetic","resumen":"Background: Nano-hydroxyapatite particles have better bioactivity than the coarse crystals. So, they can be utilized for engineered tissue implants with improved efficiency over other materials. The development of materials with specific bioactive characteristics is still under investigation.  Methods: The surface properties of four hydroxyapatitematerials templated by differentmicelle-polymer structured network are studied. The synergistic interaction of each block copolymer in contact with CTAB rod-like micelles results in crystalline HAp nano-rods of 25-50 nm length organized in hierarchical structures with different micro-rough characteristics.  Results: It was observed that the material in vitro bioactivity strongly depends on the surface structure while in a minor extent on their Ca\/P ratio. So, MIII and MIV materials with Skewness parameter Rsk N 2.62 favored the formation on their surfaces of net-like phase with a high growth kinetic constant; while MI and MII (Rsk = 2.62) induced the appearance of spherulitic-like structures and a growth rate 1.75 times inferior. Material biocompatibility was confirmed by interaction with rat calvarial osteoblasts.  Conclusions: The different structures growth is attributed to a dissimilar matching of crystal planes in the material and the apatite layer formed. In specific synthesis conditions, a biocompatible material with a Ca\/P ratio close to that for the trabecular bone and a morphology that are considered essential for bone-bonding was obtained.  General significance: The creation of implantable devices with a specific bioactive characteristic may be useful to manipulate the attachment of cells on mineral coating directly affecting the stability and life of the implant. This article is part of a Special Issue entitled: Protein trafficking & Secretion.","palabras_clave":"HYDROXYAPATITE|BIOACTIVITY|NANO-RODS|OSTEOBLAST-VIABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64375,"idioma_id":4,"titulo":"EFFECTS OF PREPARATION METHODS OF ORGANOCLAYS WITH POLYVINYL ALCOHOL IN THEIR COMPATIBILITY WITH HDPE. THERMAL STABILITY","resumen":"In this paper, the effect of polyvinyl alcohol addition on organoclay obtained by in situ polymerization and subsequent alcoholysis of polyvinyl acetate is studied. The incorporation of the monomer is carried out following two procedures. The first is the incorporation of the monomer by direct contact between the monomer and the organoclay. The second procedure involves a first step of impregnating the organoclay with propyl alcohol and the subsequent incorporation of the monomer. Both procedures consist of the polymerization in situ via a free radical mechanism. PVA modified clays showed substantial improvements in thermal properties with respect to the organoclay (MMTHDTMA), corresponding to the greater stability to clay obtained by direct contact with the monomer, PVAMMT\/HDTMA (VA). The clays prepared by both methods were incorporated into a HDPE matrix by melt blending, to study the effect of the method of preparing the organoclay with PVA in the polymer\/clay compatibility and the effect on their thermal properties. The material characterization techniques were performed using X-ray diffraction (XRD), infrared spectroscopy (FTIR), thermal analysis (TG, DTG, DSC) and transmission electron microscopy (TEM). The results show a clear improvement in the HDPE thermal properties with the addition of organoclays containing PVA and allow a discussion of effect of preparation on compatibility and improved thermal properties.","palabras_clave":"POLYVINYL ALCOHOL|MONTMORILLONITE|ORGANOCLAY|HDPE|NANOCOMPOSITES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64376,"idioma_id":4,"titulo":"IMPROVING BARRIER PROPERTIES OF HDPE USING PVA AS CLAY MODIFIER","resumen":"Abstract: In this work it was prepared nanocomposites of high density polyethylene (HDPE) with different loads of modified organoclay with polyvinylalcohol (PVA). Modified organoclay was obtained by ?in situ? polymerization of vinyl acetate with an organoclay and the nanocomposites were prepared by melt blending. The structure and morphology of nanocomposites were studied by XRD, SEM and TEM. The heat stability was measured by TGA and DSC. The barrier properties were evaluated by testing of cyclohexane pervaporation and the surface properties were obtained by determination from the contact angle using three solvents at ambient atmosphere. TEM results showed the different types of nanocomposite structures that were obtained with clay layers in the polymer matrix depending on the load of clay incorporated into the polymer matrix. The permeation experiments confirmed that the barrier properties evaluated by cyclohexane pervaporation were remarkably improved and increased in the thermal resistance for HDPE\/ organoclay modified with PVA materials compared with pristine HDPE. Results from all studies showed that the addition of modified organoclay has changed the macroscopic properties of nanocomposites, as compared to that pure HDPE. This can be attributed to the different interaction of PVA with HDPE\/filler.","palabras_clave":"nanocomposites.|pervaporation|HDPE|PVA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64377,"idioma_id":4,"titulo":"Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4","resumen":"Purpose Serum supplementation has shown to have beneficialeffects on in vitro bovine embryo development. However,it is often assumed that serum supplementation mayproduce mitochondrial damage and this damage would generatelipid accumulation, a major obstacle for cryopreservation.The aim of the present study is to investigate theprevious assumptions in early embryonic stages.Methods We considered in vitro produced bovine embryosfrom day 1 to 4 of development, which were grown inpresence of serum from days 1, 2 or 3 or in absence of it.Electron transmission micrographs allowed us to quantify thearea occupied by lipid droplets and by the different mitochondrialtypes to evaluate serum effect. Using confocal microscopywe analyzed mitochondrial activity and location.Results We found no evidence of lipid droplets accumulationor mitochondrial degeneration or reduction of mitochondrialarea in serum supplemented media. Further, our resultssuggest that events of mitochondrial proliferation are takingplace even in serum supplemented media.Conclusions Serum does not produce lipid accumulation ormitochondrial damage in bovine embryos from 2 to 16 cells.When serum was added to embryo culture medium on day 3of development, there were ultrastructural signs of a beneficialeffect for embryo development. The lack of serum untilday 3 may also avoid the unnecessary exposure to potentiallyinhibitory factors present on it.","palabras_clave":"Embryo|Serum|Lipid droplet|Mitochondria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64378,"idioma_id":5,"titulo":"Modelo dinámico del comportamiento eléctrico del corazon","resumen":"El objetivo de este trabajo es brindar conceptos y técnicas empleadas para analizar, modelar y simular la actividad eléctrica del corazón, a través de un estímulo eléctrico mediante la carga y descarga de un condensador, con el fenómeno implícito de almacenamiento de energía eléctrica asistido por un osciloscopio en el procesamiento de datos, permitiéndole afianzar los conceptos físicos en un aprendizaje significativo para facilitar y transferir los conocimientos adquiridos a otros contextos de su quehacer profesional.Se desarrolló un modelo matemático empleando la Dinámica de Sistemas parademostrar que el comportamiento del marcapaso artificial puede describirse como la carga y descarga de un condensador dependiente de factores dinámicos del sistema.","palabras_clave":"ELECTRICIDAD|ELECTRONICA|DINAMICA DE SISTEMAS|MODELO MATEMATICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64379,"idioma_id":5,"titulo":"Ser pobre, ir a la escuela... voces que resisten desde una narración singular","resumen":"Actualmente se reconoce a la pobreza como un problema multicausal, quedando pendiente superar cualquier  enfoque reduccionista.  Esto se vincula a los debates sobre la exclusión\/inclusión, vulnerabilidad, como situaciones que generan imágenes negativas en los espacios donde se desenvuelve y desarrolla una persona pobre. Partimos de la perspectiva de que la significación de una práctica y representación que de ella tienen los sujetos involucrados no es natural o inmanente, ni opcional, sino que incluye un conjunto de marcos interpretativos compartidos por determinados grupos sociales, supuestos que diseñan en relación con lo social. Particulares modos de experimentar la pobreza, la educación de sus hijos, el cómo vivir. En este contexto, para las familias el que sus hijos accedan a la escuela puede tener diferentes valoraciones. Esta investigación busca indagar sobre los significados y sentidos construidos alrededor de la pobreza, haciendo foco en las experiencias educativas vivenciadas por los sujetos pobres. Los principales resultados evidencian la persistencia de determinadas representaciones respecto al problema. Las representaciones identificadas estructuradoras de las estrategias de intervención, dan cuenta de los sentidos que adquiere la educación para los agentes de la comunidad educativa (directivos, maestros, padres y niños), el lugar de los actores en el espacio social y la interacción entre escuela y familia. Proponemos examinar la pobreza mediante una metodología cualitativa recogida por pautas flexibles difícilmente cuantificado, como lo es el discurso. Esto nos permite estructurar las visiones de la pobreza y la educación, desde una narración singular tal como es pensada y vivida por los sujetos.","palabras_clave":"POBRES|VOCES|RESISTEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64380,"idioma_id":5,"titulo":"Modelo Matemático con Retardo de un Fenómeno Físico","resumen":"Aquí pretendemos dar un paso inicial en la aplicación de las ecuaciones diferenciales con retardo, a través de resultados obtenidos en el desarrollo de un laboratorio en Física, relacionado al fenómeno de transferencia de calor.","palabras_clave":"MODELO MATEMATICO|ECUACION DIFERENCIAL CON RETARDO|DINAMICA DE SISTEMAS|EXPERIMENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64381,"idioma_id":4,"titulo":"Glycation of heat-treated Beta-lactoglobulin: Effects on foaming properties","resumen":"In this paper, the effect of glucose-glycation of beta-lactoglobulin (monomer 88%, dimer 12%) on foaming properties has been studied in 20mM phosphate buffer at pH 6.8. The results obtained by electrophoresis, circular dichroism and fluorescence quenching showed the presence of small amounts of aggregates of higher molecular weight, while only slight changes in the tertiary structure flexibility of the glycated protein were detected. The improvement on foaming properties  (foamability and foam stability) after glycation was promoted by heat treatment in the dry state (96 h at 50 °C) rather than by sugar conjugation. On the other hand, treated betalactoglobulin was obtained by heating a protein solution (55 mg mL-1 in 20 mM phosphate buffer at pH 6.8) at 85 °C for 3 min. This heated protein sample (monomer 51%, dimer 27%, trimer 19%) formed foams with good stability. After glycation, spectroscopic measurements demonstrated that no significant changes were introduced in protein conformation, since the substrate used was composed by unfolded species. However, the additional presence of oligomers of protein found after sugar conjugation, decreased the volume foam stability, probably due to steric impediment that diminished the viscoelastic stiffness of the interfacial film. The variation of volume foam stability caused by the different treatments assayed in this work, resulted from the action of these treatments on disproportionation rather than on liquid drainage from the foam.","palabras_clave":"BETA.LACTOGLOBULIN|GLUCOSE-GLYCATION|HEAT TREATMENT|FOAMING PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64382,"idioma_id":5,"titulo":"Automatic Detection of Team Roles in Computer Supported Collaborative Work","resumen":"Computer systems designed to support teamwork   are environments that enable and enhance collaboration among   users. These systems, named groupware, incorporate techniques   that facilitate communication and coordination among team   members to improve group performance. However, as not all   team members are identical, it is important to study users?   characteristics to build more productive teams.   Team Roles Theory allows obtaining the best possible   performance taking into account individual skills, combining the   weaknesses of each role with the strengths of other team   members. Therefore, it is essential in group formation to   consider mind the role each member is capable of playing.   Currently, each team member has to complete lengthy   questionnaires to determine his team role.   We propose an approach for automatic detection of team roles   from the observation of user interactions in CSCW systems.   Particularly, we apply Artificial Intelligence to obtain the team   role that best suits a user?s characteristics and behaviours. To   achieve this, the performance of various classification algorithms   was analyzed. The results show that our approach can detect   team roles with high degrees of precision.","palabras_clave":"TEAM ROLES|TEAMWORK|GROUPWARE|CSCW"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64383,"idioma_id":5,"titulo":"Actualidad  de  la  capacitación  en  Inteligencia  de Negocios  brindada  por organizaciones  latinoamericanas","resumen":"P { margin-bottom: 0.21cm; text-align: justify; }P.western { font-size: 11pt; }P.cjk { font-size: 11pt; }P.ctl { font-family: 'Times New Roman',serif; font-size: 11pt; }A:link { color: rgb(0, 0, 255); }A.ctl:link { font-family: 'Times New Roman',serif; }            En la actualidad no se ha  identificado un programa de capacitación que defina con claridad las  estrategias didácticas y pedagógicas adecuadas para la formación  de usuarios finales de soluciones de Inteligencia de Negocios  (BI)  en el ámbito de organizaciones de la provincia de Misiones  (Argentina). Este trabajo presenta un relevamiento de las ofertas de  capacitación en BI en Latinoamérica, e identifica un conjunto de  características de las alternativas de capacitación ofrecidas, con  el objetivo de permitir la generación de planes de capacitación  adecuados para la realidad de las organizaciones regionales.","palabras_clave":"educación|capacitación|inteligencia de negocios|usuarios finales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64384,"idioma_id":4,"titulo":"The Language of Evaluation: Paralinguistic features as a phonological domain for appraisal","resumen":"In this paper we explore the relationship between the language of evaluation and the use of paralinguistic features in reading aloud, relating Eggins & Slade´s (1997) categories of appraisal with Brown´s (1990) taxonomy of paralinguistic features. We mainly concentrate on two categories of appraisal: 1- affect, that is the expression of emotional states, and 2- amplification, that is the reader?s aloud grading of attitudes and emotions. We present an analysis of part of The Story of Tilly, a story for children beautifully read aloud by Jackie Torrence. It seems to us that this association between paralinguistic features and appraisal may constitute a phonological domain for the description of the language of evaluation beyond the lexico-grammatical level.","palabras_clave":"LANGUAGE|EVALUATION|APPRAISAL|PARALINGUISTIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64385,"idioma_id":5,"titulo":"Efecto del calor aportado en recargues nanoestructurados base hierro","resumen":"En los últimos años se han desarrollado consumibles de soldadura que depositan recubrimientos duros de aleaciones base hierronanoestructuradas de gran resistencia al desgaste abrasivo. Las resistencias al desgaste erosivo y abrasivo están controladasprincipalmente por la composición química y la microestructura. A su vez, la microestructura del metal depositado puede presentarvariaciones con el procedimiento de soldadura empleado, especialmente en relación al aporte térmico. Los parámetros operativosque definen el aporte térmico (tensión, corriente y velocidad de soldadura) afectan aspectos como la geometría del cordón (ancho,penetración y sobremonta) y la dilución con el material base. El propósito de este trabajo fue estudiar el efecto del calor aportadosobre las características geométricas del cordón, la dilución y la evolución microestructural de una aleación nanoestructurada basehierro, depositada por FCAW. Se soldaron muestras con aportes térmicos de entre 0,5 y 3,5 kJ\/mm. Sobre cada cupón soldado se realizóun relevamiento dimensional, se analizó la composición química y se caracterizó la microestructura usando microscopías óptica yelectrónica de barrido y difracción de rayos X. También se midieron la microdureza del depósito, el tamaño de cristalita y el grado dedilución. Se observó una gran influencia de las condiciones de proceso sobre la geometría del cordón. La dilución varió entre un 30 yun 40%, la microdureza del depósito se encontró entre 800 y 870 HV1 y el tamaño de cristalita osciló entre 105 y 130 nm, en funciónde las variables de proceso empleadas. Las mayores durezas y los menores tamaños de cristalita se obtuvieron con el menor aportetérmico, asociado a una menor dilución.","palabras_clave":"RECUBRIMIENTOS DUROS|NANOMATERIALES|CALOR APORTADO|MICROESTRUCTURA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64386,"idioma_id":4,"titulo":"Assessment of cellular effect in the vicinity of Mg, AZ31 and ZEK100 alloys","resumen":"The degradation process of Mg based resorbable implant materials induces biocompatibility problems detected at the implantation site. Cellular effects assessment showed that adverse effects may be associated to Mg and alloying ions when the limit of tolerance is surpassed1. It is worth noting that the concentration of released ions is not uniform in space and that toxic effect may be dependent on the distance from the source of ions.  Metal salts and extracts obtained after the immersion of metal samples in culture media are frequently used to assess toxicity of the released metal ions in cell lines. However, the effect of the local environment close to the metal cannot be evaluated in this way. The aim of this work is to assess the change of the viability and the capacity of forming colonies of the cells in presence of Mg-based metal disks of different compositions with the distance from the source of ions.   Results showed a strong cytotoxic effect in case of pure Mg, probably due to local changes related to the higher corrosion rate (pH, hydrogen burbles, high Mg ions concentration)1. In case of ZEK100 the decrease in the colony diameters suggests that its alloying elements may produce a more significant effect on the proliferation capacity of cells than AZ31.","palabras_clave":"BIODEGRADABLE|BIOADSORBIBLE|MG ALLOY|CELL CULTURES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64387,"idioma_id":4,"titulo":"Double-differential cross sections for ionization of H2O by fast bare O ions: Comparison with continuum-distorted-wave eikonal-initial-state calculations in prior and post forms","resumen":"We have measured the double-differential cross sections (DDCS) for electron emission in ionization of H2O molecules under the impact of 4.5-MeV\/u O8+ ions. The data were collected between 1 and 600 eV, in an angular range of 20&#8728;\u2013150&#8728; by using an electrostatic hemispherical analyzer. In the experiment we used the H2O vapor in a static gas condition which allowed us to deduce the absolute value of the cross sections. The single-differential cross sections (SDCS) and the total cross sections have also been obtained. The DDCS as well as the SDCS spectra are compared with the continuum-distorted-wave eikonal-initial-state (CDW-EIS) calculations for both the prior as well as the post forms of the scattering matrix. The initial state is represented within the complete neglect of differential overlap approximation, where the molecular orbitals are expressed in terms of atomic orbitals of the atomic constituents. The overall agreement with the CDW-EIS model is quite good as far as the energy dependence is concerned. The prior form of the model is found to provide a better understanding of the data compared to the post version. In particular, excellent agreement between the theory and experiment has been observed for the angular distribution data at forward angles.","palabras_clave":"IONIZATION|EXPERIMENTAL|DISTORTED WAVE|WATER MOLECULE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64388,"idioma_id":4,"titulo":"Electrochemical assessment of biomaterial surfaces using mini-cell systems","resumen":"A review of the electrochemical measurements made using a mini-cell electrochemical system is presented.","palabras_clave":"CORROSION|TECHNIQUE|ELECTROCHEMISTRY|ELECTROCHEMICAL SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64389,"idioma_id":4,"titulo":"Comparison of Frequency Bands Using Spectral Entropy for Epileptic Seizure Prediction","resumen":"Introduction. Under the hypothesis that the uncontrolled neuronal synchronization propagates recruiting more and more neurons, the aim is to detect its onset as early as possible by signal analysis. This synchronization is not noticeable just by looking at the EEG, so mathematical tools are needed for its identification. Objective. The aim of this study is to compare the results of spectral entropies calculated in different frequency bands of the EEG signals to decide which band may be a better tool to predict an epileptic seizure. Materials and Methods. Invasive ictal records were used. We measured the Fourier spectrum entropy of the electroencephalographic signals 4 to 32 minutes before the attack in low, medium and high frequencies. Results. The high-frequency band shows a markedly rate of increase of the entropy, with positive slopes and low correlation coefficient. The entropy rate of growth in the low-frequency band is practically zero, with a correlation around 0.2 and mostly positive slopes. The mid-frequency band showed both positive and negative slopes with low correlation. Conclusions. The entropy in the high frequencies could be predictor, because it shows changes in the previous moments of the attack. Its main problem is the variability, which makes it difficult to set the threshold that ensures an adequate prediction.","palabras_clave":"spectral entropies |high-frequency band |Seizure Prediction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64390,"idioma_id":4,"titulo":"Response of grass species to different fire frequencies in semi-arid rangelands of central Argentina","resumen":"The study was undertaken to quantify the effect of different controlled fire frequencies on foliar cover, density,  individual basal area, and mortality of the most common perennial grass species in the semi-arid rangelands of the southern  Caldenal in central Argentina over a 20-year period. Cover of bare soil was also assessed. The study comprised three fire  treatments: (i) high fire frequency (controlled burns every 3?5 years; HFF); (ii) low fire frequency (controlled burns every  8 years; LFF); and (iii) unburned control (C). Fire treatments, regardless of frequency, induced an increase in foliar cover and  density in desirable grasses, no changes in intermediate grasses, and a decrease in undesirable grasses. Individual basal area  tended to be higher for desirable grasses and lower for intermediate and undesirable grasses when subject to fire. Most of the  species under study exhibited higher mortality rates in the HFF treatment than in the LFF andCtreatments. The results of the  study suggest that recurrent controlled burns of moderate intensity may favour the herbage production of desirable perennial  grasses. This, in turn, assuming appropriate grazing management, may have a beneficial impact on livestock production.  Nevertheless, given the effects of fire on the cover of bare soil and mortality of grasses, further research is needed in order to  determine the appropriate fire frequency in terms of rangeland sustainability.","palabras_clave":"fire|frequency|grasses|rangeland"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64391,"idioma_id":5,"titulo":"La Investigación Etnomatemática en Catamarca","resumen":"La Etnomatemáticas surge como una inquietud de reconocer el desarrollo de las diversas técnicas matemáticas en diferentes ambientes. Si bien consideramos una matemática universal, existen variadas técnicas abordadas desde las diversas culturas, como consecuencia de los conocimientos existentes en tales grupos étnicos, los conocimientos luego incorporados y la adaptación de estos a ese ambiente. Las experiencias en investigación en nuestro ambiente son muy recientes, como lo es la Etnomatemática dentro de la Matemática misma. Es nuestra intención aquí observar y analizar este punto de partida y la expectativa que puede surgir en las distintas ciencias afines.","palabras_clave":"ETNOMATEMATICA|RESEÑA HISTORICA |MATEMATIZACION|EXPERIMENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64392,"idioma_id":4,"titulo":"Including species interactions in resource selection of guanacos and livestock in Northern Patagonia","resumen":"                      Species occurrence depends on both environmental and  biotic factors (species interactions). Consideration of species interactions  when estimating functions of population distribution is unusual, and may be crucial  to understand and predict how species use space and resources. In this study, we  combine resource selection probability functions (RSPFs) with a model selection  approach based on information theory to evaluate how biotic (interspecific  interactions) and abiotic (environmental) factors affect resource selection of guanacos Lama  guanicoe and livestock (goats, sheep, cattle and horses) in two  seasonal periods. We first test different a priori hypotheses of the  environmental effects on guanacos and livestock occurrence (i.e. foraging, predation\/topography and  human effect hypotheses), then we assess model performance with independent  data, and finally we use validated models of each species as predictors of the  interaction between them. In all seasons, L. guanicoe occurrence was  influenced by both environment and livestock interactions, especially small livestock (goats and  sheep). Guanacos selected for habitats characterized by high temporal  variability in plant productivity and away from potential human contact. In all seasons, L.  guanicoe was negatively related to the RSPF of small livestock, but the  reverse was not the case, suggesting that L. guanicoe avoids sites used by goats and  sheep. In contrast, livestock was mainly affected by environmental variables related to  human presence and was not affected by the interactions with herbivores.  Contrary to our predictions, goats and sheep were also associated with less  productive sites, probably indicating strong degradation of the sites to which they are  restricted. Our results suggest a spatial segregation between L. guanicoe and  domestic herbivores throughout the year, which is explained by competitive interactions  of L. guanicoe with small livestock but also in response to vegetation  productivity and human pressure. This study shows the importance of including species interaction  effects in habitat modeling.  ","palabras_clave":"INTERACCIONES ENTRE ESPECIES|SELECCIÓN DE RECURSOS|LAMA GUANICOE|ZONAS ARIDAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64393,"idioma_id":4,"titulo":"Spatial and seasonal dynamic of abundance and distribution of guanaco and livestock: insights from using density surface and null models","resumen":"Monitoring species abundance and distribution is a prerequisite when assessing species status and population viability, a difficult task to achieve for large herbivores at ecologically meaningful scales. Co-occurrence patterns can be used to infer mechanisms of community organization (such as biotic interactions), although it has been traditionally applied to binary presence\/absence data. Here, we combine density surface and null models of abundance data as a novel approach to analyze the spatial and seasonal dynamics of abundance and distribution of guanacos (Lama guanicoe) and domestic herbivores in northern Patagonia, in order to visually and analytically compare the dispersion and co-occurrence pattern of ungulates. We found a marked seasonal pattern in abundance and spatial distribution of L. guanicoe. The guanaco  population reached its maximum annual size and spatial dispersion in spring-summer, decreasing up to 6.5 times in size and occupying few sites of the study area in fallwinter. These results are evidence of the seasonal migration process of guanaco populations, an increasingly rare event for terrestrial mammals worldwide. The maximum number of guanacos estimated for spring (25951) is higher than the total population size (10000) 20 years ago, probably due to both counting methodology and  population growth. Livestock were mostly distributed near human settlements, as expected by the sedentary management practiced by local people. Herbivore distribution was non-random; i.e., guanaco and livestock abundances co-varied negatively in all seasons, more than expected by chance. Segregation degree of guanaco and small-livestock (goats and sheep) was comparatively stronger than that of guanaco and large-livestock, suggesting a competition mechanism between ecologically similar herbivores, although various environmental factors could also  contribute to habitat segregation. The new and compelling combination of methods used here is highly useful for researchers who conduct counts of animals to simultaneously estimate population sizes, distributions, assess temporal trends and characterize multi-species spatial interactions.","palabras_clave":"Density surface model|Null models|Abundance|Spatial distribution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64394,"idioma_id":4,"titulo":"?Magnetic and Elastic Anisotropy in Magnetorheological Elastomers using Nickel-based Nanoparticles and Nanochains?.","resumen":"Nickel (Ni) based nanoparticles and nanochains were incorporated as fillers in polydimethylsiloxane (PDMS) elastomers and then these mixtures were thermally cured in the presence of a uniform magnetic field. In this way, macroscopically structured-anisotropic PDMS-Ni based magnetorheological composites were obtained with the formation of pseudo-chains-like structures (referred as needles) oriented in the direction of the applied magnetic field when curing. Nanoparticles were synthesized at room temperature, under air ambient atmosphere (open air, atmospheric pressure) and then calcined at 400&#8201;°C (in air atmosphere also). The size distribution was obtained by fitting Small Angle X-ray Scattering (SAXS) experiments with a polydisperse hard spheres model and a Schulz-Zimm distribution, obtaining a size distribution centered at (10.0&#8201;±&#8201;0.6) nm with polydispersivity given by &#963;&#8201;=&#8201;(8.0&#8201;±&#8201;0.2) nm. The SAXS, X-ray powder diffraction, and Transmission Electron Microscope (TEM) experiments are consistent with single crystal nanoparticles of spherical shape (average particle diameter obtained by TEM: (12&#8201;±&#8201;1) nm). Nickel-based nanochains (average diameter: 360&#8201;nm; average length: 3&#8201;&#956;m, obtained by Scanning Electron Microscopy; aspect ratio&#8201;=&#8201;length\/diameter &#8764; 10) were obtained at 85&#8201;°C and ambient atmosphere (open air, atmospheric pressure). The magnetic properties of Ni-based nanoparticles and nanochains at room temperature are compared and discussed in terms of surface and size effects. Both Ni-based nanoparticles and nanochains were used as fillers for obtaining the PDMS structured magnetorheological composites, observing the presence of oriented needles. Magnetization curves, ferromagnetic resonance (FMR) spectra, and strain-stress curves of low filler´s loading composites (2% w\/w of fillers) were determined as functions of the relative orientation with respect to the needles. The results indicate that even at low loadings it is possible to obtain magnetorheological composites with anisotropic properties, with larger anisotropy when using nanochains. For instance, the magnetic remanence, the FMR field, and the elastic response to compression are higher when measured parallel to the needles (about 30% with nanochains as fillers). Analogously, the elastic response is also anisotropic, with larger anisotropy when using nanochains as fillers. Therefore, all experiments performed confirm the high potential of nickel nanochains to induce anisotropic effects in magnetorheological materials.","palabras_clave":"MAGNETIC|ANISOTROPY|ELASTOMER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64395,"idioma_id":4,"titulo":"Aberrant DNA methylation in breast cancer cells","resumen":"The epigenome is regulated by a large number of macromolecular machines that are dynamically  involved in various processes, including DNA methylation, histone modification and  non-coding RNA signals, all of them working together to regulate the proper expression of  the genome. Thus, in contrast with the genome, whose sequence is carefully conserved during  cell life, the epigenome is highly dynamic. The epigenomic modifications are acquired  during normal cell differentiation, replicated during mitosis and passed to daughter cells. A  fundamental epigenetic attribute is that this plasticity occurs in response to environmental  signals. It is therefore now accepted that the environment influences modifications in the cellular  transcriptome through the epigenome. In developmental and evolutionary terms, the  regulation of gene expression through epigenomic modifications is an advantageous shortcut and a highly conserved mechanism. However, it implies an increased risk for misregulation, as, for example, aberrant epigenomic modifications associate with the development of different  human diseases, i.e. lupus, asthma, neurological diseases and cancer. Although epigenetic alterations in breast cancer have been deeply studied and discussed in the last decades, apparently contradictory results are yet often observed. Consequently, in this review, we will briefly discuss the latest findings of aberrant DNA methylation in breast tumorigenesis. Emphasis will be given to the discussion of the idea that different environments could explain paradoxical biological and pathobiological behaviors in individual patients and thus should be taken into consideration for the design and implementation of diagnosis, prognosis and predictive biomarkers.","palabras_clave":"BREAST CANCER|PROMOTER METHYLATION|EPIGENETICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64396,"idioma_id":5,"titulo":"Metodología de evaluación del nivel de competitividad de las PyMEs","resumen":"Desde el grupo de investigación, en el cual los autores son articipantes, se pretende aportar al fortalecimiento de la competitividad de las PyMES de Olavarría, a través del estudio de sus cadenas de valor. El mismo se aborda por medio de dos enfoques complementarios, uno basado en actividades y otro basado en recursos.  Como paso previo a posteriores investigaciones, se selecciona una muestra no probabilística de empresas metalmecánicas. Para comprender y analizar la cadena de valor de las PyMEs de referencia se desarrolla un cuestionario que indaga sobre los recursos asociados a cada actividad componente de la cadena de valor. En pos de profundizar el análisis y definir el nivel de madurez alcanzado por la empresa en términos de competitividad, el presente estudio propone una metodología para evaluar todas las actividades de la cadena de valor ponderando los recursos que la empresa dispone para cada una de ellas.","palabras_clave":"Cadena de valor|enfoque basado en actividades y recursos|competitividad|PyMEs"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64397,"idioma_id":4,"titulo":"Ab initio LSDA and LSDA+U study of pure and Cd-doped cubic lanthanide  sesquioxides","resumen":"The electronic, structural, and hyperfine properties of pure and Cd-doped lanthanide (Ln) sesquioxides with the cubic bixbyite structure (Ln2 O3 , Ln ranging from La to Lu) have been studied using the full-potential augmented plane wave plus local orbital (APW + lo) method within the local spin density approximation (LSDA) and the Coulomb-corrected LSDA + U . In the case of the pure systems, our calculations show that LSDA + U gives a better representation of the band structure compared to LSDA. The predicted equilibrium structures and the electric field gradient (EFG) tensor at Ln sites were calculated and compared with those obtained by means of hyperfine techniques and with theoretical results obtained in In2 O3 , Sc2 O3 , and Lu2 O3 reported in the literature. The origin of the EFG at Ln sites and the role played by the 4f electrons on this quantity are discussed. In the case of the Cd-doped systems, the APW + lo method (also within LSDA and LSDA + U ) was applied to treat the electronic structure of the doped system. The role of the Ln 4f electrons on the EFG at Cd impurity sites, and other variables like structural distortions induced by the Cd impurity, were investigated in detail and are discussed and compared with available experimental results. An excellent agreement between the experimental and calculated EFGs was found for all Cd-doped systems.","palabras_clave":"LANTHANIDE|PAC|APW+LO|SEMICONDUCTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64398,"idioma_id":4,"titulo":"Prognostic Implications of Cytogenetic Features in Myelodysplastic Syndromes","resumen":"Myelodysplastic Syndromes (MDS) are a heterogeneous group of hematological diseases characterized by refractory cytopenia(s) and variable risk of leukemic progression. Cytogenetic analysis is important in day-to-day clinical practice helping to define subgroups of MDS patients who share similarities in the course of the disease. There are recurring aberrations affecting chromosomes 5, 7, 8, and 20. While all of them do not suggest a therapeutic approach, their presence has been considered as a risk indicator since the original international prognostic scoring system (IPSS) was published. The most recent cytogenetic stratifications tried to find the prognostic significance of less frequent alterations which have been longer included in the intermediate group. Moreover, monitoring of karyotype changes is suggested to evaluate cytogenetic response to treatments and the acquisition of new aberrations associated to an unfavorable outcome. This review focuses on different cytogenetic risk stratifications that have been published during the past twenty years and the molecular background of the most relevant chromosomal findings.","palabras_clave":"Síndromes Mielodiasplasicos|Citogenética|Pronostico|Cariotipo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64399,"idioma_id":4,"titulo":"Effects of coypu (Myocastor coypus) abundances and diet selection on a wetland of the Patagonian steppe","resumen":"We assessed diet selection, impact on vegetation, and explored habitat relationships with marsh birds of coypus(Myocastor coypus) in a steppe lagoon in Argentinean Patagonia. In two consecutive springs, abundance and spatial use of the coypus and nesting marsh birds were estimated by direct counts. The coypu was a selective consumer with seasonal variations in food items, and Myriophyllum sp. and Schoenoplectus californicus dominated its diet. Coypus and marsh birds showed a differential spatial use when rushes cover was high. However, when rushes cover decreased by coypu browsing, there was a similar use of space, and marsh birds were displaced to nest on the open water and other poorly protected areas of the rushes. Our results suggest that high abundances of coypu can have a detrimental effect on wetland ecosystems. Systematic monitoring and evaluation of their effects on wetlands in recently colonized areas is recommended.","palabras_clave":"Diet selection|Coypu impact|Invasive species|Pest"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64400,"idioma_id":4,"titulo":"Foraging behavior and spatial use of a rock specialist: the southern vizcacha (Lagidium viscacia), and the exotic European hare (Lepus europaeus) in rocky outcrops of northwestern Patagonia, Argentina","resumen":"The southern vizcacha (Lagidium viscacia) and  the exotic European hare (Lepus europaeus) are two  medium-sized herbivores that inhabit rocky outcrops in  Patagonian steppe. These species overlap in diet and spatial  use at medium distances from rocky outcrops in summer.  We evaluated the spatial use through feces distribution in  winter and determined seasonal foraging intensity in relation  to the distances from rocky outcrops in order to elucidate  how these herbivores use food and spatial resources in food  scarcity periods. The vizcacha utilized the habitat close to  rocky outcrops (40 m. However, in winter, at medium distances from rocky  outcrops, there was partial spatial overlap because hares´  activities were closer to rocky outcrops. Foraging intensity  increased significantly in areas used by the vizcacha closer  to rocky outcrops when food availability decreased, and the  grasses Stipa speciosa, Poa sp., and Festuca pallescens  were strongly foraged. In contrast, foraging intensity  showed no changes in further distances to rocky outcrops  and more use by the hare. The spatial and feeding behavior  of the vizcacha, restricted to vicinity of rocky outcrops,  showed high vulnerability to food availability changes. In  resource scarcity situations, the spatial opportunistic behavior  of the hare and the overlap in diet with the vizcacha  constitutes a threat to this native herbivore. It is necessary to  monitor populations of hare, since high densities could lead  to food competition, impacting the small colonies of the  southern vizcacha.","palabras_clave":"Alien species|Browsing|Feeding distances|Spatial relations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64401,"idioma_id":4,"titulo":"CONTINUOUS APPLICATION OF DIRECT SOWING: TRAFFIC EFFECT ON SUBSOIL COMPACTION AND MAIZE (Zea mays L.) YIELDS IN ARGENTINEAN PAMPAS","resumen":"Our main objective was to evaluate the effect of subsoil compaction on maize (Zea mays L.) yields. This work was performed on clayey soil and on loamy Entic Haplustoll soil. Three types of direct sowing (DS) equipment (heavy load (HL), medium load (ML) and light load (LL)) were utilised. We hypothesised that the application of DS over five continuous years would cause subsoil compaction and that maize yields would be affected. The cone index (CI), root dry matter per plant (RDM), dry matter per plant (DMP) and maize yields (MY) were measured. The results show that after five years, the CI values produced by the HL treatment were higher in clayey soil than in loamy soil, which decreased maize yields. At site 1, the highest average RDM values were found in 2005; the measurements for the LL and ML treatments were 45.9 and 43.1 g plant-1, respectively, whereas the highest value in the HL treatment was 35.6 g plant-1. For site 2, the highest RDM values were found in 2006; the measurements for the LL and ML treatments were 42.6 and 38.2 g plant-1, respectively, whereas the highest value in the HL treatment was 30.2 g plant -1. The average DMP measurement (site 1) was 150 g plant-1 in the LL treatment, followed by 147 g plant-1 in ML and 137 g plant-1 in HL. Site 2 values were 143, 137 and 130 g plant -1 in LL, ML and HL, respectively. At both sites, the HL treatment resulted in a significantly lower maize yield (14.3 % and 11 % for sites 1 and 2, respectively) compared to the ML and LL treatments. The main conclusions were as follows: when clayey and loamy soil was trafficked with between loads of 144 - 177.9 kN for five years, the cone index was increased in the subsoil. These high CI values caused by the combination of a high axle load and ground pressure would have affected the maize yields.","palabras_clave":"CONE INDEX|HEAVY AXLE LOAD|TRACTOR TRAFFIC|PLANTER MACHINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64402,"idioma_id":4,"titulo":"Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn","resumen":"After alcohol exposure through a standard Lieber andDeCarli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells.Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced.The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH\/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.","palabras_clave":"Alcohol|Acetaldehyde|Uterine Horn|Oxidative Stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64403,"idioma_id":5,"titulo":"Actividades predominantes a nivel nacional, regional y local de las pymes industriales argentinas","resumen":"ResumenLa creciente importancia e interés que han ido adquiriendo las Pequeñas y Medianas empresas (PyMEs) en la actividad económica del país, ha originado la realización de diversos estudios, tanto de instituciones públicas como privadas, que a través de encuestas y registros han caracterizado diferentes temáticas para este tipo de empresas.En este contexto, el Departamento de Ingeniería Industrial de la Facultad de Ingeniería perteneciente a la Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), ubicado en la ciudad de Olavarría, se encuentra actualmente desarrollando un proyecto de investigación denominado ¨Cadena de valor e impacto de las actividades clave en la conducta competitiva de las PyMEs¨ con el objeto de efectuar un diagnóstico de sus cadenas de valor y generar propuestas de mejora que impacten positivamente en la competitividad y sustentabilidad de tales empresas.Consecuentemente, la relevancia del presente estudio se focaliza en determinar las actividades predominantes de las PyMEs argentinas, a nivel nacional, regional y local, de forma tal de fundamentar la elección de la población objeto de estudio del citado proyecto.","palabras_clave":"PyMEs| Industria Manufacturera|Estudio sectorial"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64404,"idioma_id":5,"titulo":"LEISHMANIASIS VISCERAL EN LA ARGENTINA NOTIFICACIÓN Y SITUACIÓN VECTORIAL (2006-2012)","resumen":"La leishmaniasis visceral (LV) en la Argentina se notifica obligatoriamente a través del Sistema Nacional de Vigilancia de la Salud (SNVS). El análisis de la notificación muestra un total de 103 casos distribuidos en cuatro provincias, siendo Misiones la de mayor casuística con un total de 80 casos en 15 localidades, desde mayo 2006 hasta julio de 2012. Los niños de 0 a 15 años de edad son el grupo con mayor  incidencia. En el año 2004 fue registrado por primera vez el riesgo de LV urbana endémica en la provincia de Formosa tras el hallazgo del vector Lutzomyia longipalpis. En el año 2006 la provincia de Misiones registra casos de LV humana, LV canina, y la provincia de Corrientes en el verano 2008-2009. En Santiago del Estero los casos de LV humana y LV canina en el año 2008 estuvieron asociados posiblemente a vectores secundarios. En el año 2010 Lu. longipalpis fue hallado en Chajarí, Entre Ríos, mostrando su dispersión hacia el sur. Por ello, para conocer la distribución del riesgo, entre enero y abril del 2011 se realizó búsqueda sistemática del vector de LV en 13 localidades de las provincias de Entre Ríos, Santa Fe y Santiago del Estero, totalizando 275 trampas\/noche. Se comprobó la colonización de Lu. longipalpis, en la localidad de Chajarí. En Santiago del Estero no se encontró Lu. longipalpis por lo que Migonemyia migonei sigue incriminada como vector putativo de LV en esta región.","palabras_clave":"leishmaniasis visceral|notificación|situación\tvectorial"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64405,"idioma_id":5,"titulo":"Actualidad en Leishmaniasis canina","resumen":"ResumenLos autores presentan actualizan la situación epidemiológica, los problemasatinentes al diagnóstico y las herramientas de control y prevención de la grave zoonosis en la población canina de la Argentina, y presentan una crónica del 5º Congreso Mundial de Leishmaniasis realizada en mayo 2013 en Brasil.","palabras_clave":"LEISHMANIA|EPIDEMIOLOGIA|WORLDLEISH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64406,"idioma_id":4,"titulo":"Raman spectroscopy of GeSe and AgGeSe thin films","resumen":"The structural properties of Agx(Ge0.25Se0.75)100-x thin films (where x=0, 7, 10, 15, 20 and 25 at. %) were studied. The films were prepared by pulsed laser deposition using bulk glass targets of the studied ternary system and deposited onto microscope slides. Their amorphous structures were confirmed by XRD (X-ray Diffraction). The effect of the silver content on the films structures was analysed by Raman spectroscopy. Typical Raman vibration modes were observed in the Ge0.25Se0.75 binary film: Ge-Se corner-sharing tetrahedra mode (CS) at 199 cm-1, edge sharing tetrahedra mode (ES) at 217 cm-1, and Se-Se mode at 255 cm-1. In the Agx(Ge0.25Se0.75)100-x ternary thin films, the same modes were observed but with a red shift. An intensity reduction of the ES and Se-Se bands with the increase of silver content was also observed. In a previous work [1], morphology and optical properties of the films had been studied. Refractive index and the optical band gap energy had been determined. A decrease in the optical band gap and an increase in the refractive index had been observed with the increase of Ag content. Structural studies were applied with the aim of explaining the optical behavior.","palabras_clave":"RAMAN SPECTROSCOPI|CHALCOGENIDE GLASSES|THIN FILMS|AG GE SE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64407,"idioma_id":4,"titulo":"Boar seminal plasma exosomes: effect on sperm function and protein identification by sequencing","resumen":"Plasma seminal de mamíferos contiene vesículas membranosas (exosomas), con un alto contenido de colesterol y esfingomielina y una composición de proteína compleja. Su función fisiológica es incierto debido a la estabilización de los espermatozoides y los efectos de activación han sido reportados. Para analizar un papel modulador putativo de exosomas semen sobre la actividad de los espermatozoides en el jabalí, se examinaron los efectos de estas vesículas en varios parámetros funcionales espermáticas. Además, las proteínas exosome jabalíes fueron secuenciados y se exploró su incorporación a los espermatozoides. Los espermatozoides de cerdo se incubaron bajo condiciones que inducen la capacitación, que se manifiesta como un aumento de la fosforilación de tirosina, la pérdida de colesterol y una mayor fluidez en las membranas apicales, y la capacidad de someterse a la reacción del acrosoma lisofosfatidilcolina inducida. Después de establecer este conjunto de capacitación que dependen de los parámetros funcionales, el efecto producido por exosomas cuando está presente durante o después se analizó capacitación del esperma. Los exosomas inhibieron el aumento capacitación dependiente de eflujo de colesterol y la fluidez en las membranas apicales, y la desaparición de un polipéptido fosforilado 14-kD. En contraste, la reacción acrosómica (espontánea y lisofosfatidilcolina inducida) no se vio afectada, y la unión a la zona pelúcida de ovocitos esperma se redujo sólo cuando las vesículas estuvieron presentes durante la coincubación de gametos. Los liposomas con una composición de lípidos similar a la presente en exosomas imitaban estos efectos, excepto el de zona pelúcida de unión. Interacción entre exosomas y el esperma se confirmó por transferencia de la actividad aminopeptidasa. Además, la principal proteína de exosoma, identificado como actina, apareció a asociar con el esperma después de coincubación. Exosome composición tenía un predominio de las proteínas estructurales (actina, plastina, ezrina, y Condensina), enzimas, y varios polipéptidos de plasma específicas seminales porcinas (por ejemplo, spermadhesins). La transferencia de proteínas a partir de exosomas a los espermatozoides y su capacidad para bloquear el flujo de salida de colesterol apoya una interacción directa entre estas vesículas y el esperma, mientras que la inhibición de algunas de las características que dependen de capacitación sugiere una función estabilizadora para exosomas en semen porcino.","palabras_clave":"Exosome|Prostasome|Sperm capacitation|Boar"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64408,"idioma_id":4,"titulo":"Evaluation of Stretcher Alignment in  Radiotherapy using Computed Tomography","resumen":"Currently, a wider and more frequent use of Computed Tomography (CT) volumes are used for planning the radiotherapy treatment of oncological patients. These image volumes are instrumental in positioning the patient and the precise incidence of radiation fields. Any error in such a process will adversely affect the result of the treatment and, consequently, the health of the patient. Therefore, quality control is mandatory throughout this process. As regards image acquisition, the controls are typically executed manually, implying a tedious repetitive task. This problem spurred the need to develop an algorithm that allows eliminating this issue while it evaluates and corrects the main concern as well. Particularly, an algorithm for automatic control of the entire positioning process is proposed here which prevents errors from deviation on both the longitudinal and transversal axes of the arrangement. The alignment will vary according to the anatomical part to be treated and the type of stretcher support, among other aspects. The application was developed by resorting to free software tools (GLP) and the libraries for processing, segmenting and recording of medical images (ITK). The algorithm detects these deviations on both axes by applying threshold methods, morphologic operations and Hough´s Transform. This algorithm is currently operative in the Medical Image Processing Server of the Nuclear Medicine School Foundation (FUESMEN) of Mendoza, Argentina","palabras_clave":"Quality Control|Medical Image Processing|Radiotherapy"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64409,"idioma_id":4,"titulo":"Evaluation of Cytochrome P450 Activity in Field Populations of Cydia pomonella (Lepidoptera: Tortricidae) Resistant to Azinphos Methyl, Acetamiprid, and Thiacloprid","resumen":"The Rio Negro and Neuquén Valley is the most important apple and pear growing region in Argentina. Cydia pomonella L. (Lepidoptera: Tortricidae), the main fruit-tree pest is being controlled by azinphosmethyl (AzMe), acetamiprid (Acet), and thiacloprid (Thia) among other insecticides. The objective of this study was to evaluate the response of neonate larvae of codling moth to these three insecticides and on the role of cytochrome P450 monooxygenase in their toxicity. All  Þeld populations presented signiÞcantly lower mortality to a discriminating concentration (DC) of Acet and AzMe. In addition, 13 of the 14 populations showed signiÞcantly lower mortality to DC of Thia. Most of the Þeld populations (71%) showed signiÞcantly higher 7-ethoxycoumarine O-deethylase activity compared with the laboratory-susceptible strain. While positive signiÞcant correlation (r = 0.59) was found between Thia and AzMe mortalities at the DC level, no signiÞcant correlations were detected between Acet and Thia (r = 0.35) or Acet and AzMe (r = 0.12). However, Acet and Thia mortalities were signiÞcantly correlated to the percentage of individuals exhibiting 7-ethoxycoumarine  O-deethylase activity activities higher than the mean upper 95% conÞdence limit of the susceptible strain (r = 0.52 and r = 0.63, respectively).","palabras_clave":"CODLING MOTH|NEONATE|INSECTICIDE RESISTANCE|DIAGNOSTIC CONCENTRATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64410,"idioma_id":4,"titulo":"SIMULTANEOUS DETERMINATION OF QUALITY PARAMETERS IN BIODIESEL\/DIESEL BLENDS USING SYNCHRONOUS FLUORESCENCE AND MULTIVARIATE ANALYSIS","resumen":"An analytical method was developed to determine four quality parameters (Biodiesel percentage, CetaneNumber, Heat of Combustion Gross and Color) in biodiesel\/diesel blends through a simple synchronous fluorescencespectrum of the samples.For this purpose, chemometrics models based on fluorescence spectra and PetroSpect data obtained frommixtures of biodiesel\/diesel were built. A variable selection by the successive projections algorithm (SPA)was used in order to obtain simple multiple linear regression (MLR) models based on a small subset of wavelengths.The SPA-MLR results were compared with a partial least squares (PLS) full spectrum regression.The best values found for the root mean square error of prediction using external validation were 0.37% (w\/w)for the biodiesel in diesel, 0.5 for cetane number, 0.013 MJ\/kg for heat of combustion and 0.1 for color.","palabras_clave":"Biodiesel \/Diesel Blends|Synchronous fluorescence|Gross heat of combustion|Cetane number"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64411,"idioma_id":4,"titulo":"Quantification of uncertainty in mercury waste water analysis at different concentration levels and using information from proficiency testwith a limited number of participants","resumen":"Measurement uncertainty is one of the most required parameter sofanalyticalquality in environmental decision-making.Severalapproacheshavebeenreportedforestimatinguncertaintyinanalytical measurements.Theuseofproficiencytest(PT)isanalternative,notonlyforassessingtheperformance of individualanalyticallaboratories,butalsoforestimatingtheconcordanceofananalyticalmethod and theirmeasurementuncertainties.HerewedevelopaPTschemeforalimitednumberofanalytical laboratoriesthattookpartintheanalysisoftotalmercuryinsampleswithcomplexmatrix(chlor-alkali process wastewatereffluent)inabsenceofreferencesofthehighestmetrologicalhierarchy(e.g. primarymethod,primaryCRM).Twoin-housereferencematerials(IHRMs)werepreparedatdifferent levelsofanalyteconcentrationandthehomogeneityrequiredwasverifiedfortheintendeduse.Asetof parametricandrobuststatisticaltestswereappliedtoevaluatetheassignedvaluesofeachIHRM. MetrologicalcompatibilityassessmentofPTresults,evaluation of individual and global performance of the laboratories,anddeterminationofuncertaintyoftheanalyticalmeasurementswereevaluatedin this restrictedstudyscenario.Between-laboratorydifferenceswerefoundnotsignificant(a¼0.05). For both concentrationlevelswecomputedarelativestandarduncertaintyof30%forthetotalHgcontent. a¼0.05). For both concentration levels we computed are lativestandard uncertainty of 30% for the total Hg content.","palabras_clave":"Proficiency testing|Wastewater analysis|Mercury wastes|Measurement uncertainty"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64412,"idioma_id":4,"titulo":"Arbuscular mycorrhizal fungi are directly and indirectly affected by glyphosate application","resumen":"Glyphosate is a systemic non-selective herbicide, the most widely used in the world. Alongside with itsuse in agricultural and forestry systems, this herbicide is used in grasslands in late summer with theaim of promoting winter species with the consequent increase in stocking rate. However, its effects onnon-target organisms, such as arbuscular mycorrhizal fungi (AMF), are unclear. Arbuscular mycorrhizalfungi (AMF) colonize the root of more than 80% of terrestrial plants, improving their growth and survival,and therefore playing a key role in ecosystem structure and function. The aim of this work was to inves-tigate the possible pathways through which glyphosate application affects AMF spores viability and rootcolonization in grassland communities. Our hypothesis is that glyphosate application can damage AMFdirectly (through contact with spores and external hyphae) or indirectly through the changes it generateson host plants. The experiment had a factorial array with three factors: (1) plant species, at two levels(Paspalum dilatatum and Lotus tenuis), (2) doses of glyphosate, at three levels (0 l ha&#8722;1, 0.8 l ha&#8722;1and 3 l ha&#8722;1), and (3) application site, at two levels: soil (direct pathway) and plant foliage (indirect pathway).Spore viability was reduced even under the lowest glyphosate rate, but only when it was applied on thesoil. Total root colonization for both species was similarly decreased when glyphosate was applied toplant foliage or on soil, with no difference between 0.8 and 3 l ha&#8722;1. The number of arbuscules was 20%lower when glyphosate was applied on plant foliage, than when it was applied on the soil. Our findingsillustrate that glyphosate application negatively affects AMF functionality in grasslands, due to differentcauses depending on the herbicide application site. While, under field conditions, the occurrence of directand\/or indirect pathways will depend on the plant cover at the time of glyphosate application, the con-sequences of this practice on the plant community structure will vary with the mycorrhizal dependenceof the species composition regardless of the pathway involved.","palabras_clave":"AMF PROPAGULES |NO TARGET ORGANISMS |ROOT COLONIZATION|SPORE VIABILITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64413,"idioma_id":5,"titulo":"Hongos arbusculares (Glomeromycota) en la rizosfera de Atriplex lampa en dos ambientes salinos de Córdoba:  influencia de la profundidad en la colonización radical y presencia de morfoespecies.","resumen":"Atriplex lampa es un arbusto halófito de la provincia fitogeográfica Chaqueña que constituyeun recurso forrajero a lo largo de todo el año. Las plantas halófitas se pueden beneficiar al asociarse con los hongos micorrícico arbusculares (HMA), ya que le proveen resistencia contra la salinidad y la sequía. En general, los estudios acerca de los HMA están restringidos a los primeros centímetros del suelo y existe poca información sobre su presencia a mayores profundidades. El objetivo de este trabajo fue estudiar la colonización, la concentración radical e identificar las morfoespecies de HMA en la rizosfera de A. lampa en dos ambientes salinos del norte de la provincia de Córdoba, durante dos estaciones del año, y en cinco profundidades del suelo. Aunque no se encontraron arbúsculos, A. lampa presentó colonización radical por HMA en todas las profundidades. El mayor valor de colonización micorrícica y de concentración radical se observó en las Salinas Grandes. Se identificaron 20 morfoespecies de HMA pertenecientes a los géneros: Acaulospora, Ambispora, Claroideoglomus, Diversispora, Funneliformis, Glomus, Septoglomus y Scutellospora. Este es el segundo registro en Argentina de la presencia de HMA en raíces de A. lampa. Es necesario realizar estudios que evalúen la respuesta de A. lampa frente a la inoculación con HMA para confirmar la funcionalidad de la simbiosis.","palabras_clave":"hongos micorrícico arbusculares|suelos salinos|Atriplex lampa|profundidad del suelo| estacionalidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64414,"idioma_id":5,"titulo":"Tolerancia In vitro de Fusarium solani  y Fusarium semitectum (Anamorfos, Ascomycota) a la exposición a desinfectantes de superficie utilizados en acuicultura.","resumen":"In vitro tolerance of Fusarium semitectum and Fusarium solani (anamorphic, Ascomycota) exposed to surface disinfectant commonly used in aquaculture. An in vitro evaluation of the antifungal effect of acetic acid (AcH), buffered acetic acid (AcH+AcNa), potassium iodide (KI)  and Iodopovidone (IP); on the growth rate (GR) of Fusarium solani (Mart.) Sacc. 1881 and Fusarium semitectum Berk. & Ravenel, 1875 was performed. Both fungal species were isolated from eggs of the Argentinian silverside Odontesthes bonariensis (Cuvier & Valenciennes, 1835). The studied substances are commonly used in fish farming as surface disinfectants. The tested concentrations of this substances exhibited antifungal activity for both strains at concentrations higher than their 96h median lethal concentrations (LC50-96h) for the treated eggs; concentrations below the (LC50-96h) exhibited little or no effect.","palabras_clave":"Ácido acético|inhibición del crecimiento in-vitro|Iodóforos|ovas de Odontesthes bonariensis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64415,"idioma_id":5,"titulo":"Propiedades físicas de maíz colorado duro influenciadas por el secado artificial","resumen":"El objetivo del presente trabajo fue comparar el secado natural (a temperatura ambiente) con el artificial (a 70 y 90°C), que es el utilizado en el tratamiento convencional que se realiza en plantas de acopio, y su efecto en el índice de fisurado, las propiedades físicas asociadas a la  dureza y el ángulo de reposo. Este experimento se realizó en dos híbridos ´flint´ de distinta dureza (Cóndor y Mill 522), en comparación con un híbrido dentado (Aw 190).","palabras_clave":"PROPIEDADES FISICAS|SECADO ARTIFICIAL|GRANOS|MAIZ"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64416,"idioma_id":5,"titulo":"Triquinosis en la provincia de Buenos Aires: alimentos involucrados en brotes de la enfermedad","resumen":"  La triquinosis es una zoonosis endémica en la provincia de Buenos Aires. En el periodo 2004-2012  se registraron 74 brotes de la enfermedad con 1546 casos humanos confirmados, los alimentos involucrados  en la transmisión de la enfermedad fueron principalmente elaborados con carne de cerdo; la ingesta de  embutidos secos (chorizo seco y longaniza), sin control sanitario, ocasionó la mayoría de los casos (87%).  La prueba de digestión artificial demostró ser una técnica útil para el diagnóstico de la infección en carne  fresca y productos procesados infectados aún con muy bajas cargas parasitarias. No obstante su utilización  ha sido limitada, pues solamente se recibieron muestras para estudio en el 33.8%, de los brotes registrados.","palabras_clave":"TRICHINELLOSIS|PROVINCIA DE BUENOS AIRES|PRUEBA DE DIGESTION PEPTICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64417,"idioma_id":4,"titulo":"Oxidative Stress and Antimicrobial Activity of Chromium(III) and Ruthenium(II) Complexes on Staphylococcus aureus and Escherichia coli","resumen":"The prevalence of antibiotic resistance has resulted in the need for new approaches to be developed to combat previously easily treatable infections. The main aim of this work was to establish the potential of the synthetic alfa-diimine chromium(III) and Ruthenium(II) complexes (where the alfa-diimine ligands are bpy = 2,2-bipyridine; phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2´,3´-c]-phenazine) like [Cr(phen)3]3+, [Cr(phen)2(dppz)]3+, [Ru(phen)3]2+ and [Ru(bpy)3]2+ as antibacterial agents by generating oxidative stress. The [Cr(phen)3]3+ and [Cr(phen)2(dppz)]3+ complexes showed activity against Gram positive and Gram negative bacteria with minimum inhibitory concentrations (MICs) ranging from 0.125 µg\/mL to 1 µg\/mL, while [Ru(phen)3]2+ and [Ru(bpy)3]2+ does not exhibit antimicrobial activity against the two bacterial genera studied at the concentration range used. When ciprofloxacin was combined with [Cr(phen)3]3+ for the inhibition of Staphylococcus aureus and Escherichia coli, an important synergistic effect was observed, FIC 0.066 for S. aureus and FIC 0.064 for E. coli. The work described here shows that chromium(III) complexes are bactericidal for S. aureus and E. coli. Our results indicate that alfa-diimine chromium(III) complexes may be interesting to open new paths for metallodrug chemotherapy against different bacterial genera since some of these complexes have been found to exhibit remarkable antibacterial activities.","palabras_clave":"Chromium(III) complexes|oxidative stress|antimicrobial activity|ciprofloxacin|Staphylococcus aureus|Escherichia coli"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64418,"idioma_id":5,"titulo":"Caracterización de yogur firme elaborado con leche ovina","resumen":"En Argentina, el destino principal de la leche ovina es la elaboración de quesos. Sin embargo, su alto contenido en extracto seco la convierte en una materia prima de excelente calidad para la elaboración de yogur. Se estudió la elaboración de yogur con leche ovina conservada por un periodo de dos meses a -18°C. Luego de ser descongelada (baño termostático a 40 ºC), se analizó microbiológicamente y se procedió con la elaboración de yogur firme por método tradicional, se evaluaron sus características microbiológicas, físico-químicas y composicionales. Finalmente, se realizó un análisis sensorial (juez afectivo). Los análisis de la leche ovina descongelada no mostraron variación en sus características físico-químicas al compararlos con los de leche ovina fresca; sin embargo, se observó un alto recuento de mesófilos viables, debido al proceso de descongelación. El yogur obtenido presentó un 7,58% de proteínas y 10,03% de grasa. En cuanto a sus características microbiológicas, se obtuvieron valores menores a 1 UFC\/g para coliformes, hongos y levaduras, pudiéndose considerarlo como un producto microbiológicamente inocuo. En cuanto a los datos del análisis sensorial, se obtuvo una aceptabilidad alta (94%) del producto. La leche ovina congelada sería adecuada para la elaboración de un yogur muy aceptable. ","palabras_clave":"YOGURT|LECHE OVINA|TECNOLOGIA LECHERA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64419,"idioma_id":4,"titulo":"A theoretical and empirical framework for constructing culture-specific stigma instruments for Chile","resumen":"-","palabras_clave":"ESTIGMA SOCIAL|CULTURA|SALUD MENTAL|AMÉRICA LATINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64420,"idioma_id":5,"titulo":"Transmisión transgeneracional del trauma psicosocial en comunidades indígenas de Argentina: percepción del daño en el pasado, el presente y acciones autoreparatorias","resumen":"-","palabras_clave":"SALUD INDÍGENA|POBLACIÓN INDÍGENA|TRASTORNOS POR ESTRES POSTRAUMATICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64421,"idioma_id":4,"titulo":"Effect of hydrothermal treatment on Cr-SiO2 mesoporous materials","resumen":"Cr\/SiO2 (MCM-41) materials were prepared by the sol-gel method by hydrolysis of tetraethylortosilicate (TEOS) using cetyltrimethylammonium bromide (CTAB) as a template. The effect of hydrothermal treatment between 120-220°C on the structural properties was studied. Well ordered Cr\/MCM-41 with a very narrow pore size distribution was obtained after hydrothermal treatment from RT to 150°C. Materials synthesized at higher temperatures (200 or 220°C) showed the collapse of the ordered mesoestructure to give mesoporous Cr\/SiO2 with an open surface. Superficial Cr3+\/Cr6+ ratio was determined by XPS over materials hydrothermically synthesized at different temperatures, and calcined in air at 300°C.The hydrothermal treatment improved the chromium to silica interaction, promoting a higher resistance to form Chromium (VI) sites. The chromium (III) ions incorporated to the silica structure developed acid sites responsible for the 2-PrOH dehydration.","palabras_clave":"mesoporous|Chromium|sol-gel|catalysts"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64422,"idioma_id":4,"titulo":"Degree of fetal growth restriction associated with schizophrenia risk in a national cohort","resumen":"BACKGROUND:Accumulating evidence suggests that fetal growth restriction may increase risk of later schizophrenia but this issue has not been addressed directly in previous studies. We examined whether the degree of fetal growth restriction was linearly related to risk of schizophrenia, and also whether maternal pre-eclampsia, associated with both placental dysfunction and poor fetal growth, was related to risk of schizophrenia.METHOD:A population-based cohort of single live births in the Medical Birth Registry of Norway (MBRN) between 1967 and 1982 was followed to adulthood (n=873 612). The outcome was schizophrenia (n=2207) registered in the National Insurance Scheme (NIS). The degree of growth restriction was assessed by computing sex-specific z scores (standard deviation units) of \u2018 birth weight for gestational age\u2019 and \u2018 birth length for gestational age\u2019. Analyses were adjusted for potential confounders. Maternal pre-eclampsia was recorded in the Medical Birth Registry by midwives or obstetricians using strictly defined criteria.RESULTS:The odds ratio (OR) for schizophrenia increased linearly with decreasing birth weight for gestational age z scores (p value for trend=0.005). Compared with the reference group (z scores 0.01\u20131.00), the adjusted OR [95% confidence interval (CI)] for the lowest z-score category (<x3.00) was 2.0 (95% CI 1.2\u20133.5). A similar pattern was observed for birth length for gestational age z scores. Forty-nine individuals with schizophrenia were identified among 15 622 births with pre-eclampsia. The adjusted OR for schizophrenia following maternal pre-eclampsia was 1.3 (95% CI 1.0\u20131.8).CONCLUSIONS:Associations of schizophrenia risk with degree of fetal growth restriction and pre-eclampsia suggest future research into schizophrenia etiology focusing on mechanisms that influence fetal growth, including placental function.","palabras_clave":"DEGREE|FETAL|GROWTH|SCHIZOPHRENIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64423,"idioma_id":4,"titulo":"Range extension of Tympanoctomys barrerae (Lawrence 1941) (Rodentia: Octodontidae) in Patagonia and southernmost record","resumen":"We report the southernmost record for the octodontid rodent T. barrerae, which is also the second recording locality for this species in Patagonia. &nbsp;The new locality is placed in S Neuquén province, extending the known range of the species about 150 km SW from the nearest previous record.&nbsp; Although T. barrerae is considered a highly specialized rodent of salar flats in Monte desert, the record reported here came from an arbustive rocky environment belonging to the Monte-Patagonia ecotone. ","palabras_clave":"N\/C"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64424,"idioma_id":4,"titulo":"Quark-hybrid matter in the cores of massive neutron stars","resumen":"Using a nonlocal extension of the SU(3) Nambu-Jona Lasinio model, which reproduces several of the key features of Quantum Chromodynamics, we show that mixed phases of deconfined quarks and confined hadrons (quark-hybrid matter) may exist in the cores of neutron stars as massive as around 2.1M_sun. The radii of these objects are found to be in the canonical range of &#8764; 12 &#8722;13 km. According to our study, the transition to pure quark matter does not occur in stable neutron stars, but is shifted to neutron stars which are unstable against radial oscillations. The implications of our study for the recently discovered, massive neutron star PSR J1614\u20132230, whose gravitational mass is 1.97±0.04 M_sun, are that this neutron star may contain an extended region of quark-hybridmatter at it center, but no pure quark matter  ","palabras_clave":"quark matter|neutron stars"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64425,"idioma_id":5,"titulo":"'Vida cotidiana y participación ciudadana:la sociedad comodorense durante la Guerra de Malvinas'","resumen":"EL trabajo se propone indagar sobre las maneras en las que los habitantes de la ciudad de Comodoro Rivadavia vivieron la Guerra de Malvinas, haciendo hincapié en la vida cotidiana, las voces que se expresaron y la participación ciudadana, tratando de vislumbrar cómo los comodorenses elaboraron la experiencia de la guerra y la capitalizaron.","palabras_clave":"MALVINAS|VIDA COTIDIANA|COMODORO RIVADAVIA|PARTICIPACIÓN CIUDADANA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64426,"idioma_id":5,"titulo":"Consumo de Sustancias y Escuela: Un estudio en adolescentes  Escolarizados de Córdoba","resumen":"En esta investigación se propuso identificar el consumo de sustancias psicoactivas según tipo de colegio y describir el consumo según jornada de estudio, sexo, exigencia académica y disciplina escolar. Es un estudio es observacional y analítico.Se trabajó a partir de la Segunda Encuesta Nacional a Estudiantes de Enseñanza Media. El análisis descriptivo se efectuó a partir de las medidas de resumen, el exploratorio a través del procesamiento estadístico con análisis bivariado (p<0,05) y análisis factorial de correspondencia múltiple.Se identificó que fuman más cigarrillos los estudiantes que asisten a escuelas públicas que a escuelas privadas, que mayor es el riesgo de consumo de marihuana y cocaína en los alumnos que asisten a escuelas públicas que en privadas, que menor es el consumo de sustancias en el turno matutino y que el consumo de alguna sustancia ilícita se asocia a problemas de comportamiento y repitencia escolar.","palabras_clave":"TRASTORNOS RELACIONADOS CON EL USO DE SUSTANCIAS PSICOACTIVAS|ADOLESCENTES|EDUCACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64427,"idioma_id":5,"titulo":"Formación Docente e integración interdisciplinaria","resumen":"-","palabras_clave":"FORMACIÓN DOCENTE|INTEGRACIÓN|INTERDISCIPLINARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64428,"idioma_id":5,"titulo":"Leer en áreas de conocimiento: la experiencia en la Facultad de Ciencias Médicas, UNC.","resumen":"En el contexto universitario los alumnos ingresantes se confrontan con géneros discursivos que suponen prácticas de lectura específicas, no siempre explícitas (Carlino, 2005). De ahí que interpretar correctamentelos contenidos de los textos de especialidad requiera competencias estratégicas, léxicogramaticales y discursivas, de las que los estudiantes podrán apropiarse si son acompañados por docentes que modelen e incentiven los modos de lectura esperados en el nivel superior y, particularmente, del objeto de estudio. Este ensayo presenta y describe el proyecto llevado a cabo durante el Ciclo de Nivelación en la Facultad de Cs. Médicas (Universidad Nacional de Córdoba) para implementar modos de ayudar a los aspirantes a leer en el contexto universitarioen forma comprensiva y reflexiva. La experiencia contempló cuatro momentos: capacitación docente, clase a los aspirantes, diseño de actividades de lectocomprensión e integración de contenidos curriculares con actividades que propiciaron modos de lectura propios del contexto académico. Este artículo de reflexión basado en investigación argumenta en favor de que los profesores expliciten los saberes que la cultura académica presupone, para lo que precisan, a su vez, el apoyo de lasinstituciones.","palabras_clave":"ENSEÑANZA DE LECTURA|EDUCACIÓN SUPERIOR|PROYECTO INTEGRADOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64429,"idioma_id":5,"titulo":"Análisis de la mortalidad por suicidio en niños adolescentes y jóvenes. Argentina 2005-2007.","resumen":"Objetivos. Analizar la mortalidad por suicidio  en niños, adolescentes y jóvenes de 5 a 24 años,  según sexo, método utilizado, lugar de ocurrencia  y jurisdicción en la Argentina, en el período  2005-2007.  Método. Se utilizaron las Bases de Datos de los Informes  Estadísticos de Defunción del Ministerio  de Salud de la Nación. Se calcularon frecuencias  y tasas de mortalidad por suicidio especí&#64257;cas por  100 000 habitantes, con empleo de la clasi&#64257;cación  CIE-10-(X60-X84). El análisis estadístico se  realizó mediante datos categorizados.  Resultados. El total absoluto de muertes por  suicidio en el grupo de 5 a 24 años fue de 861  en 2005, 897 en 2006 y 815 en 2007. En el período  2005-2007 se produjeron 6 óbitos en menores de  10 años. En el grupo de 10 a 24 años las muertes  por suicidio fueron más frecuentes en varones  (p <0,01). El análisis por grupo de edad y sexo  mostró que, en varones, la mortalidad incrementa  con la edad (p <0,01) y, en mujeres, el grupo  de 15 a 19 años fue el más prevalente (p <0,05).  El método utilizado con mayor frecuencia fue  el ahorcamiento (p <0,01) y el lugar de ocurrencia  más prevalente el hogar (p <0,01). Las tasas  de suicidio más elevadas del país fueron en las  jurisdicciones de Santa Cruz, Salta y Jujuy, y la  tasa más baja en Santiago del Estero.  Conclusión. El suicidio en la población de adolescentes  y jóvenes de la Argentina constituye  un problema de salud más frecuente en los varones.  Las jurisdicciones del norte y sur del país  muestran las tasas más elevadas.","palabras_clave":"MORTALIDAD|SUICIDIOS|NIÑOS|ADOLESCENTES|JOVENES|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64430,"idioma_id":10,"titulo":"Consumo de alcohol según características sociodemográficas en jóvenes de 18 a 24 años","resumen":"Objetivo: identificar o consumo de álcool, de acordo com as características sociodemográficas em   jovens de 18 a 24 anos, provenientes da província de Córdoba, Argentina, e que participaram   do Levantamento Nacional de Fatores de Risco. Método: foi realizado um estudo quantitativo,   analítico e transversal com uma amostra de 240 jovens. O consumo de álcool e as características   sociodemográficas foram analisados mediante a aplicação da análise bivariada, razão de risco e   intervalos de confiança. Resultados: a prevalência do consumo de álcool na vida foi de 79,17%, a   prevalência-ano 72,91% e a prevalência-mês 57,08%, o consumo excessivo episódico e o consumo   regular de risco correspondem a 25,55 e 24,19%, respectivamente. O consumo no último mês foi   maior entre homens (p=0,0028), jovens solteiros (p=0,0001), com educação secundária completa   (p=0,0306). Conclusão: na província de Córdoba, o uso de substâncias psicoativas, entre elas o   álcool, não tem sido foco de interesse nas investigações em anos recentes, porém, o abuso do   álcool está associado a outros fatores de risco, tais como acidentes e violência, e como fator de   comorbidade e mortalidade precoce, portanto, o consumo de álcool entre os jovens é um tema   preocupante que, quando caracterizado, deve ser tratado com ações preventivas.","palabras_clave":"Consumo de Bebidas Alcoólicas|Estudos Transversais|Adolescente|Adulto Jovem"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64431,"idioma_id":4,"titulo":"Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis","resumen":"The difficulties in preventing and treating infectious bovin keratoconjunctivitis (IBK) and the consequent impact on the cattle industry worldwide emphasize the need to better understand this infectious process along with the biology of Moraxella bovis, its primary causative agent. Although there is increasing evidence that bacterial biofilms participate in a variety of ocular infections by direct biofilm formation on the surfaces of the eye, IBK has not been considered as a biofilm-based disease so far, and even more, no information is currently available regarding the ability of M. bovis to adopt a biofilm lifestyle. In the present research, we demonstrated the capacity of M. bovis clinical isolates and reference strains to form biofilms on different abiotic surfaces and culture conditions, and provided qualitative and quantitative information on the biofilm growth and architecture of mature biofilms. In addition, our data indicated that the type IV pili play a critical role in the biofilm formation in vitro. Most significantly, we proved that through exposure to MgCl2 type IV pili are removed from the cell surface, not only preventing M. bovis biofilm formation but also disassembling preformed biofilms. These results could constitute a new approach in the understanding of M. bovis colonization process in cattle eye and\/or nasal cavity, and may aid in the development of future antimicrobial strategies for the control of IBK.","palabras_clave":"MORAXELLA|PILI|BIOFILM|VACCINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64432,"idioma_id":4,"titulo":"Cephalic morphological variation in freshwater silversides Odontesthes hatcheri and Odontesthes bonariensis in Patagonia: introgression and ecological relationships","resumen":"Formerly, Odontesthes hatcheri (Eigenmann) was present in Cuyo and Patagonia. The area was repeatedly stocked with Odontesthes bonariensis (Valenciennes) during the 1930s. In a recent survey, O. bonariensis individuals were captured mostly in northern localities whereas O. hatcheri ones were captured in the south. Based on Geometric Morphometrics and Discriminant Analysis, the percentage of O. hatcheri and O. bonariensis individuals indentified by a taxonomic key and misclassified by Discriminant Analysis was obtained and a negative correlation between the percentage of misclassified individuals of O. hatcheri and the distance to the nearest hatchery stocking silversides was found, suggesting a genetic introgression. Morphological analyses between species, between populations, and within populations pointed to the same anatomical structures, suggesting a nested variation related with environmental cues like availability of littoral shelter. The dependence between the cephalic morphology of O. hatcheri and body size would be in agreement with the trophic niche shifts of the species. Introgression adds a new threat to the already observed decline of populations of O. hatcheri and suggests that this species deserves particular consideration in terms of conservation guidelines.","palabras_clave":"Atherinopsidae|Cephalic morphology|Fish stocking|Geometric Morphometrics|Hybrids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64433,"idioma_id":4,"titulo":"The age-metallicity relationship of the Large Magellanic Cloud field star population from wide-field Washington photometry","resumen":"We analyze age and metallicity estimates for an unprecedented database of some 5.5 millionstars distributed throughout the Large Magellanic Cloud (LMC) main body, obtained from CCD Washington $CT_1$ photometry, reported on in Piatti et al. 2012. We produce a comprehensive field star Age-Metallicity Relationship(AMR) from the earliest epoch until $sim$ 1 Gyr ago.This AMR reveals that the LMC has not evolved chemically&nbsp; as either a closed-box or bursting system, exclusively, but as a combination of both scenarios that have varied in relative strength over the lifetime of the galaxy, although the bursting model falls closer to the data in general.Furthermore, while old and metal-poor field stars have been preferentially formed in the outer disk,&nbsp; younger and more metal-rich stars have mostly been formed in the inner disk, confirming an outside-in formation. We provide&nbsp; evidence for the formation of stars between 5 and 12 Gyr, during the cluster age gap, although chemical enrichment during thisperiod was minimal. We find no significant metallicity gradient in the LMC. We also find that the range in the metallicity of an LMC field &nbsp;has varied during the lifetime of the LMC. In particular, we find only a small range&nbsp;&nbsp;&nbsp;&nbsp; of the metal abundance in the outer disk fields, whereas an average range of $Delta$[Fe\/H] = +0.3 $pm$ 0.1 dexappears&nbsp; in the inner disk fields.Finally, the cluster and field AMRs show a satisfactory match only for the last 3 Gyr,&nbsp; while for the oldest ages ($&gt;$ 11 Gyr) the cluster AMR is a remarkable lower envelope to the field AMR. Such a difference may be due to the very rapid early chemical evolution and lack of observed field stars in this regime, whereas the globular clusters are easily studied.This large difference is not easy to explain as coming fromstripped ancient Small Magellanic Cloud (SMC) clusters, although the field SMC AMR is on average $sim$ 0.4 dex more metal-poor at all ages than that of the LMC but otherwise very similar.","palabras_clave":"cluster"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64434,"idioma_id":4,"titulo":"First report of Tomato Rugose Yellow Leaf Curl Virus infecting tomato in Argentina","resumen":"Tomato plants exhibiting typical symptoms of begomovirus infection, including leaf deformation, curling, and yellowing, were collected from cultivated fields in Lavalle Department, Corrientes, Argentina, in 2010. Although the number of affected plants was only 2% within a farm, the finding is of considerable importance since the white fly Bemisia tabaci is widely spread within the country, even in other southernmost areas such as the cinturón hortícola de Buenos Aires (horticultural belt around Buenos Aires). DNA isolated from infected tomato leaves collected from three symptomatic tomato plants was amplified by PCR with specific primers designed to amplify a region of component A and B of the Begomovirus genome (3). The amplified DNA fragment was sequenced and a new set of primers were designed based on the obtained sequences. A DNA fragment of about 1,300 bp was amplified and later the complete genome, which was 2,683 bp long. No fragments were obtained when template DNA was from non-infected leaf samples. The 2,683-bp fragment was annotated at the NCBI under Accession No. KC132844. Analysis by NCBI BLAST showed that it was highly homologous to DNA-A component of Begomovirus. Furthermore, the genome organization was typical of DNA-A component of bipartite New World begomovirus. The sequence had one open reading frame (ORF) on the viral-sense strand (AV1\/CP) and four ORFs on the complementary-sense strand (AC1\/Rep, AC2\/TrAp, AC3\/REn, and AC4). In order to confirm this finding, the viral genome was amplified by rolling circle amplification (RCA, TempliPhi 100 Amplification Kit, Amersham Biosciences) as described by the manufacturer instructions. The RCA full-length product was digested with XhoI generating a 2,700-bp DNA fragment, suggesting the presence of only one restriction site, in agreement with the bioinformatics analysis of the KC132844 sequence. This PCR product was used as template in PCR reactions with specific primers to DNA-A or DNA-B components. While the DNA-A primers generated the expected 1,300-bp fragment, those homologous to the DNA-B component did not generate amplifications. These results confirmed the identity of the DNA-A component of the isolate MT8. The full sequence of the DNA-A component was 94% homologous to the DNA-A sequence of the Uruguayan begomovirus Tomato Rugose Yellow Leaf Curl Virus-[U4.1] (JN381823.1). Therefore, considering our results and the criteria proposed by Fauquet (1), isolate MT8 is a new species of begomovirus described recently (2). This is the first report of TRYLCV in one of the main areas of tomato production in Argentina. This virus might be accompanying another begomovirus TYVSV that provoked yellow veins symptoms in tomato plants cultivated in the same area of Corrientes. These viruses appeared recently and concomitantly with the introduction of the white fly Bemisia spp. in the area, which is one of the main production areas of tomato and provides fresh tomatoes to the whole country, and in wintertime to the city of Buenos Aires, when the horticultural belt around Buenos Aires is not under production.","palabras_clave":"Geminivirus|Tomato|TRYLC|Virus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64435,"idioma_id":4,"titulo":"Sensory analysis of broccoli over time: Consumer defined critical attributes and evaluation of digital photographs in comparison to real product appearance","resumen":"The objectives of the present work were: (a) define the critical attribute of broccoli from a consumers?perspective and (b) for broccoli compare the trained panel sensory evaluation of real products versusthe sensory evaluation of the corresponding digital photographs. A panel of broccoli consumers evaluatedappearance, aroma and flavor acceptability of broccoli with different storage times. Appearance acceptabilitywas the only attribute where there were significant differences between storage times. Thusappearance was the critical attribute as evaluated by consumers. A panel of assessors measured theappearance of broccoli samples with different storage times using the quality scoring method (QSM). Amonth after having measured the real product the same assessors measured the appearance of digitalphotographs of the same samples using the QSM. Analysis of variance showed that there were no significantdifferences between evaluations of the real broccoli and the corresponding photograph.","palabras_clave":"BROCCOLI|CRITICAL ATRIBUTE|QUALITY SCORING METHOD|DIGITAL PHOTOGRAPHS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64436,"idioma_id":4,"titulo":"Intra-phylum and inter-phyla associations among gastrointestinal parasites in two  wild mammal species.","resumen":"A growing body of literature reveals that the interactions among the parasite community may be strong and significant for parasite dynamics. There may be inter-specific antagonistic interactions as a result of competition and cross-effective immune response, or synergistic interactions where infection by one parasite is facilitated by another one, either by an impoverishment of the host\u2019s defenses, parasite-induced selective immunosuppression, or trade-offs within the immune system. The nature of these interactions may depend on how related are the parasite species involved. Here we explored the presence of associations among gastrointestinal parasites (coccidia and helminths) in natural populations of two wild mammal species, the capybara (Hydrochoerus hydrochaeris) and the guanaco (Lama guanicoe). The associations explored were between the oocyst outputs of a selected Eimeria species and the other coccidia of that parasite community, and between Eimeria spp. and the predominant nematodes. The statistical analysis included adjustment for potential confounders or effect modifiers. In guanacos, the prevailing interactions were synergistic among the coccidia and between coccidia and nematodes (Nematodirus spp.). However, in capybaras, the interaction between nematodes (Viannaiidae) and Eimeria spp. depended on environmental and host factors. The relationship was positive in some circumstances (depending on season, year, sex, or animal size), but it appeared to become antagonistic under different scenarios. These antagonist interactions did not follow a particular seasonal pattern (they occurred in autumn, spring, and summer), but they were predominantly found in females (when they depended on sex) or in 2010 and 2011 (when they depended on the sampling year). These results suggest that the relationship between coccidia and nematodes in capybaras may be context dependent. We propose that the context-dependent immune investment documented in capybaras may be the cause of these varying interactions.","palabras_clave":"PARASITE INTERACTIONS|DISEASE ECOLOGY|INFECTION DYNAMICS|CO-INFECTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64437,"idioma_id":4,"titulo":"Evaluation of terrain corrections through FFT and classical integration in some areas of the Andes and their impact on geoidal heights","resumen":"As part of the regional geoid modeling, terrain corrections were computed in Tierra del Fuego island and in the west side of the province of Mendoza. The first place is located in the southernmost region of Argentina and Mendoza is in the center-west of this country. Considering both study areas, elevations range from 0 m to 6500 m. The classical integration of prism contribution and the 2-D FFT technique were usedto estimate terrain corrections. This study aims at discussing the results obtained by both approaches and their applicability considering their advantages and disadvantages according to the regions under investigation. The analysis allowed us to conclude that classical integration has a better performance than FFT methods, especially in the highest regions where terrain corrections can be overestimated in more than 20 mGals by FFT. Both techniques described show similar results in flat areas. Finally, the effect that the error of terrain corrections computation has on geoidal heights is also discussed and numerically tested. It is proved that an error in gravity anomalies of 20 mGals may cause up to 2 m geoid height error.","palabras_clave":"TERRAIN CORRECTIONS|CLASICAL INTEGRATION|TIERRA DEL FUEGO|MENDOZA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64438,"idioma_id":4,"titulo":"Ultrasound leaching - dispersive liquid-liquid microextraction based on solidification of floating organic droplet for determination of polybrominated diphenyl ethers in sediment samples by gas chromatography-tandem mass spectrometry","resumen":"Ultrasound leaching-dispersive liquid-liquid microextraction using   solidification of floating organic droplet (USL-DLLME-SFO) technique is   proposed for extraction and isolation of polybrominated diphenyl ethers   (PBDEs) from sediment and further determination by gas   chromatography\/tandem mass spectrometry (GC?MS\/MS). Parameters that   affect the efficiency of the procedure were investigated by a full   factorial (2k) screening design. Variables showing   significant effects on the analytical responses were considered within a   further central composite design (CCD). The optimization assays have   led to following protocol: ultrasound assisted lixiviation of 1&nbsp;g   sediment was carried out by using 1.2&nbsp;mL MeOH. Further, the analytes   were isolated from 0.4&nbsp;mL of the extract using the DLLME-SFO technique.   The microextraction was performed using 0.1&nbsp;mL MeOH, 22&nbsp;mg 1-dodecanol,   1&nbsp;mL NaCl solution 6.15&nbsp;M and 4.4&nbsp;mL ultrapure water as dispersive and   extracting solvents, medium ionic strength and dispersant bulk,   respectively. Under optimum conditions, the method exhibits good   performance in terms of linearity and precision (RSD&nbsp;&lt;&nbsp;9.2%), with   recoveries above 71% and limits of detection (LODs) within the range   0.5?1.8&nbsp;pg&nbsp;g&#8722;1 dry weight (d.w.). Method validation was   demonstrated through the analysis of environmental sediment samples in   which PBDEs were detected and quantified. The presence of BDE-47, -100,   -99 and -153 was reported within the concentration range of &lt;LOD to   29&nbsp;pg&nbsp;g&#8722;1 d.w. The proposed methodology constitutes a   suitable approach for the analysis of PBDEs in complex solid samples   requires minimum organic solvents consumption, sample manipulation, and   increases sample throughput.","palabras_clave":"POLIBROMINATED DIPHENYL ETHERS|DISPERSIVE LIQUID-LIQUID MICROEXTRACTION|SOLIDIFICATION OF FLOATING ORGANIC DROPLET|SEDIMENT|GAS CHROMATOGRAPHY - MASS SPECTROMETRY|EXPERIMENTAL DESIGN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64439,"idioma_id":5,"titulo":"Apuntes para pensar los jóvenes de hoy. Narrativas sobre los medios y subjetividades docentes?.","resumen":"Las culturas y las estéticas  contemporáneas se caracterizan  por la centralidad y el impacto de  los medios masivos de comunicación y de las nuevas tecnologías en el conjunto de procesos socioculturales e  históricos y, particularmente, en las instituciones educativas. En este trabajo  pretendemos poner en diálogo dos dimensiones de la comunicación y la educación. Una referida al lugar  de los medios y de las tecnologías en la escuela. La otra que atraviesa las percepciones y discursos de los docentes,  con la finalidad de dar cuenta, de los modos oscilantes e imprecisos en que la  educación configura las subjetividades de los jóvenes de hoy. Para abordar  estas tensiones, comenzamos haciendo referencia a las significaciones  culturales en torno a los medios de comunicación masivos como contenidos y como  estrategias pedagógicas en las instituciones educativas. Incluimos, a su vez, a  las nuevas tecnologías devenidas en  tecnologías educativas. Luego, reflexionamos sobre las formas en que las  distintas subjetividades de los docentes se tornan visibles en el ámbito  escolar, buscando poner en evidencia los procedimientos narrativos utilizados  en este intento de constituir la subjetividad del estudiante","palabras_clave":"ENSEÑANZA|MEDIOS DE COMUNICACION|NARRATIVAS|NUEVAS TECNOLOGÍAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64440,"idioma_id":4,"titulo":"Factor VIII genotype characterization of haemophilia A affected patients with transient and permanent inhibitors: a comprehensive Argentine study of inhibitor risks","resumen":"Inhibitor development against exogenous factor VIII is a severe   impairment of replacement therapy affecting 18% of Argentine patients   with severe haemophilia A (HA). To study the molecular predisposition   for inhibitor development, we genotyped 260 HA patients with and without   inhibitors, countrywide. The inhibitor-positive population (19   transients, 15 low responders, LR and 70 high responders, HR) of 104   severe-HA patients showed 59 Inv22 (intron 22 inversions), 18 small   ins\/del-frameshifts, 12 gross deletions, 12 nonsense, one splicing   defect and two missense, p.Arg531Pro and p.Leu575Pro, both LR and   thought to impair FVIII A2 domain secondary structure. In addition, a   patient with mild HA and HR showed the missense p.Glu1704Lys associated   with two neutral intronic substitutions potentially affecting the A3   domain. A case\/control study (84\/143) permitted estimation of F8   genotype-specific inhibitor risks [OR; prevalence (CI)] in severe-HA   patients classifying a high-risk group including multi-exon deletions   [3.66; 55% (19-100)], Inv22 [1.8; 24% (19-100)] and nonsense in   FVIII-LCh [1.2; 21% (7-59)]; an average risk group including single-exon   deletions, indel frameshifts and nonsense-HCh; and a low-risk group   represented by missense defects [0.14; 3% (0.6-11)]. Analysis of   inhibitor concordance\/discordance in related patients indicated   additional genetic factors other than F8 genotype for inhibitor   formation. No significant inhibitor-predisposing factors related to   FVIII product exposure were found in age- and F8 genotype-stratified   populations of severe-HA patients. In conclusion, the Argentine HA   patient series presents similar global and mutation-specific inhibitor   risks than the HA database and other published series. This   case-specific information will help in designing fitted therapies and   follow-up protocols in Argentina.","palabras_clave":"FVIII genotype|Inhibitor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64441,"idioma_id":4,"titulo":"Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo  exposure to endocrine disrupting chemicals.","resumen":"Caiman latirostris is a reptilian species that exhibits temperature-dependent sex determination (TSD). Male-to-female sex reversal can be achieved after in ovo estrogen\/xenoestrogen exposure. This is known as hormone-dependent sex determination (HSD). The amh, sox9 and sf-1 genes are involved in sex determination, sex differentiation, and steroidogenesis. The aims of this study were: (a) to establish the expression patterns of amh, sox9 and sf-1 mRNA in the gonad-adrenal-mesonephros (GAM) complexes of neonatal TSD-male and TSD-female caimans, (b) to compare the expression of these genes between TSD-females and HSD-females (born from E2-exposed eggs incubated at the male-producing temperature) and (c) to evaluate whether in ovo exposure to a low dose of E2 or bisphenol A (BPA) or to a high dose of endosulfan (END) modifies amh, sox9 or sf-1 mRNA expressions in neonatal males. The mRNA expressions of amh, sox9 and sf-1 in GAM complexes from TSD-males and TSD-females and from HSD-females were quantitatively compared by RT-PCR. A sexually dimorphic pattern of amh and sox9 mRNA expression was found, with a higher expression in TSD-males than in TSD-females. sf-1 mRNA did not differ between TSD-males and TSD-females. HSD-females exhibited a higher expression of sox9 than TSD-females. In males, increased mRNA expression of sex-determining genes was observed after in ovo exposure to END. E2 decreased sox9 but increased sf-1 mRNA expression. Changes induced by BPA were evident although not significant. These results provide new insights into the potential mechanisms that lead to the gonadal histo-functional alterations observed in caimans exposed to contaminated environments.","palabras_clave":"Bisphenol A|Endosulfan|Sex-determining genes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64442,"idioma_id":4,"titulo":"Macrolide resistance in Streptococcus pneumoniae isolated from Argentinian pediatric patients suffering from acute otitis media","resumen":"Macrolide-resistant Streptococcus pneumoniae emerged in Argentina in 1995, representing 26% of invasive infection isolates in children under 5 years old. The objectives of this study were to describe the prevalence of ermB and mefA genes in macrolide-resistant S. pneumoniae isolates from acute otitis media (AOM) and to determine their genetic relatedness. Between May 2009 and August 2010, 126 S. pneumoniae isolates from 324 otherwise healthy children with a fi rst episode of AOM were included. Twenty six of these isolates (20.6%) were resistant to erythromycin. Most frequent serotypes were: 14 (46.2%), 6A (23.1%), 19F (7.7%) and 9V (7.7%). Twenty (76.9%) carried the mefA gene, 5 (19.2%) have the ermB gene, and 1 (3.9%) both ermB + mefA. Ten clonal types were identifi ed, mostly related to Sweden15A-25\/ST782 (SLV63), CloneB6A\/ST473 and England14-9\/ST9. This is the fi rst study assessing the mechanisms of macrolide resistance in pneumococci isolates from pediatric AOM in Argentina and their genetic relatedness.","palabras_clave":"STREPTOCOCCUS|PNEUMONIAE|ACUTE OTITIS MEDIA|MACROLIDE RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64443,"idioma_id":4,"titulo":"CA-MRSA as a Cause of Lemierres Syndrome: First Report of Two Cases in Argentina","resumen":"Background: Lemierre´s syndrome (LS) is currently an uncommon disease and Fusobacterium necrophorumis responsible for two-thirds of the cases. From 1965 to the present, a total of 11 cases were reported in the literature caused by Staphylococcus aureus, one of them was recently associated to USA300 clone. Here we report two LS cases in Argentina associated with CA-MRSA. Case reports: Case 1: a 17-year-old man, without any clinically relevant data, was hospitalized in September 2011 due to right internal jugular vein and right cavernous sinus thrombosis, brain abscess and septic pulmonary emboli. MRSA was isolated in 3\/4 blood cultures. Antibiotic treatment and anticoagulation were administered with complete clinical resolution. Case 2: a 34-year-old man, without any clinically relevant data, was hospitalized in November 2011 with bilateral internal jugular vein, transverse sinus thrombosis, and septic pulmonary emboli. MRSA was isolated in 2\/2 blood cultures and BAL. The patient died after multiple complications in spite of antibiotic treatment and anticoagulation. Strains were not epidemiologically related. The type of staphylococcal chromosomal cassette (SCCmec) and PVL were determined by PCR as previously described. Genetic relationship among isolates and reference strains was established by SmaI-PFGE. Both strains were characterized as CA-MRSA, carried SCCmecIV, were PVL(+) and were not genetically related by PFGE. One of the CA-MRSA (case 2) was genetically indistinguishable by PFGE from CA-MRSA ST5-SCCmecIV PVL+, the dominantCA-MRSA clone detected in Argentina since 2000. None of these strains was related to the USA300 clone (ST8) or the Southwest Paci&#64257;c (ST30) clone. To the best of our knowledge, these represent the &#64257;rsts two cases of LS associated with CA-MRSA reported in Argentina. Comments: CA-MRSA could be an emerging cause of LS in addition to typically recognized organisms. Awareness of CA-MRSA will guide empirical antibiotic choice and may positively impact patient outcomes.","palabras_clave":"LEMIERRE|CAMRSA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64444,"idioma_id":5,"titulo":"Efectos de la ola de calor sobre la vaca Holando Argentino en el Módulo Tambo de la Facultad de Ciencias Agrarias-UNR","resumen":"Es un artículo de divulgación científica donde se exponen resultados preliminares del Proyecto de Investigación que se desarrolla en el Módulo Tambo de la F.Cs Agrarias-UNR (Zavalla)cuyo objetivo principal es evaluar el nivel de estrés en las vacas en ordeño y sus consecuencias sobre el bienestar y la salud. Para ello se analizan durante las estaciones de verano 2011-2012 y 2012-2013: parámetros fisiológicos: frecuencias cardíaca y respiratoria, temperatura corporal;  análisis de composición de la leche realizados en el Laboratorio Regional de Servicios Analíticos a través de un convenio con la Asociación del Litoral de Entidades de Control Lechero (ALECoL); registros climáticos de la Estación Agrometeorológica de Zavalla perteneciente a la red del Servicio Meteorológico Nacional y del INTA; análisis de sangre y suero realizados en el Laboratorio Centralizado del Hospital Escuela y de Inmunología de la Facultad de Ciencias Veterinarias-UNR; y datos de producción obtenidos de la Dirección del Módulo Tambo: sistema de gestión del rodeo ALPRO®. Algunos resultados preliminares corroboran que con valores de ITH superior a 72 se alteran los parámetros fisiológicos. Por ejemplo, durante una ola de calor ocurrida en el mes de febrero del 2012, donde el ITH alcanzó un valor de 80,08, los animales sufrieron estrés calórico moderado manifestando un aumento de la temperatura corporal y de la frecuencia respiratoria. Algo similar se produjo durante las olas de calor ocurridas durante enero del 2013, donde con valores de ITH entre 72 y 76 se registraron aumentos significativos de la frecuencia cardíaca.  Otro indicador de estrés calórico es la composición de la leche: grasa, proteínas,lactosa, sólidos no grasos y células somáticas. Al respecto, no se han evidenciado alteraciones que demuestren un efecto negativo a excepción del recuento de células somáticas. Sin embargo, debemos considerar por un lado, que durante el verano siempre hay un ligero aumento en los recuentos celulares somáticos, y por otro, que las cantidades halladas no son lo suficientemente elevadas como para corroborar la existencia de inflamación o infección de la glándula mamaria.  En síntesis, los resultados hallados al momento indican que los animales no han sufrido episodios de estrés calórico capaces de afectar negativamente la calidad de la leche. Probablemente debido a las características del sistema productivo, en especial al manejo nutricional que es el que mayormente determina la composición láctea; así como también, al suministro de sombra, la disponibilidad de agua limpia y fresca, y los horarios de ordeño que fueron adecuados a las condiciones climáticas imperantes. Otro factor que pudo haber resultado ventajoso fue la amplitud térmica que les permitió recuperarse durante la noche cuando el ITH disminuyó sensiblemente debido a un importante descenso de la temperatura.","palabras_clave":"ESTRÉS TÉRMICO|BOVINO LECHERO|PRODUCCIÓN LECHERA|ITH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64445,"idioma_id":4,"titulo":"Controlling Carboxyl Deprotonation on Cu(001) by Surface Sn Alloying","resumen":"We find that for adsorbed terephthalic acid (TPA) molecules surface Sn alloying deactivates the Cu(001) surface by decoupling the adsorbed molecules from the substrate. This effect is investigated for the case of the 0.5 ML phase of the Sn\/Cu(001) surface alloy by applying fast X-ray photoemission spectroscopy, scanning tunneling microscopy, near-edge X-ray absorption fine structure spectroscopy, and density functional theory calculations. The experimental results conclusively show that the deprotonation reaction of the carboxyl groups occurring in the clean Cu(001) is fully inhibited on this Sn\/Cu(001) surface alloy, which allows the molecules to form two-dimensional arrays stabilized by [OH···O] hydrogen bonds. The formed arrays exhibit a crystal structure that is practically indistinguishable from that theoretically obtained for unsupported TPA sheets, suggesting an extremely weak molecule\/substrate interaction. This is supported by DFT calculations of the adsorption energy landscape of the TPA sheets formed on the Sn\/Cu(001) template: the lateral variation of the adsorption energy (corrugation) is estimated to be less than 0.2 eV, with an adsorption energy per molecule in the range 1.6?1.8 eV and a contribution of each double [OH···O] bond of 1 eV. Finally, the performed thermal desorption experiments show that the TPA sheets remain stable on the surface alloy until their desorption. From these experiments, a value of 1.5 eV was determined for the desorption energy barrier, which is consistent with the important contribution of the [OH···O] bonds to the stability of the sheets as theoretically predicted. The results reported in this study suggest that a gradual activation of the interaction between the TPA molecules and the Cu(001) surface will also be obtained for decreasing Sn coverage.","palabras_clave":"SURFACE ALLOYING|MOLECULAR ADSORPTION|REACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64446,"idioma_id":4,"titulo":"Formation of non-substitutional beta-Sn defects in Ge1-x Snx alloys","resumen":"  p, li { white-space: pre-wrap; }          Although group IV semiconductor alloys are expected to form          substitutionally, in Ge$_{1-x}$Sn$_x$ this is true only for low          concentrations ($x&lt;0.13$).  The use of these alloys as a narrow gap          semiconductor depends on the ability to produce samples with the          high quality required for optoelectronic device applications.  In a          previous paper we proposed the existence of a non-substitutional          complex defect ($beta$-Sn), consisting of a single Sn atom in the          center of a Ge divacancy, that may account for the segregation of Sn          at large $x$. Afterwards, the existence of this defect was confirmed          experimentally. In this paper we study the local environment and the          interactions of the substitutional defect ($alpha$-Sn), the vacancy          in Ge, and the $beta$-Sn defect by performing extensive numerical          {it ab initio} calculations. Our results confirm that a $beta$-Sn          defect can be formed by natural diffusion of a vacancy around the          substitutional $alpha$-Sn defect, since the energy barrier for the          process is very small.","palabras_clave":"SEMICONDUCTOR ALLOYS|OPTOELECTRONICS|DEFECTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64447,"idioma_id":4,"titulo":"Robust Human Machine Interface Based on Head Movements Applied to Assistive Robotics","resumen":"This paper presents an interface that uses two different sensing techniques  and combines both results through a fusion process to obtain the minimumvariance  estimator of the orientation of the user?s head. Sensing  techniques of the interface are based on an inertial sensor and artificial  vision. The orientation of the user?s head is used to steer the navigation  of a robotic wheelchair. Also, a control algorithm for assistive technology  system is presented. The system is evaluated by four individuals with  severe motors disability and a quantitative index was developed, in order  to objectively evaluate the performance. The results obtained are promising  since most users could perform the proposed tasks with the robotic  wheelchair.","palabras_clave":"robotic wheelchair|sensor fusion|vision based interface|inertial sensor"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64448,"idioma_id":5,"titulo":"Diseño de un sistema de rehabilitación para miembro superior en entorno de realidad virtual","resumen":"Este trabajo describe el diseño y desarrollo de un sistema capaz de adquirir señales electromiográficas de superficie, digitalizarlas y procesarlas en una computadora personal, para ser usadas como control de un objeto de realidad virtual que representa al miembro superior. La clasificación de la intencionalidad del usuario se realiza mediante la configuración y entrenamiento de una red neuronal artificial. Luego se presenta en tiempo real la animación en realidad virtual de los movimientos realizados por el miembro superior. Los resultados para los cuatro voluntarios estudiados, indican una tasa de clasificación positiva en promedio del 75% para cada uno de ellos.","palabras_clave":"Miembro superior|Rehabilitación| Electromiografía"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64449,"idioma_id":5,"titulo":"Condiciones productivas y exigencias de calidad en la fruticultura de la Patagonia Argentina","resumen":"La  producción alimentaria está regida, a partir de los años noventa, por  requerimientos de calidad e inocuidad  que  modifican sustancialmente las condiciones productivas del eslabón primario, en  el marco de una reestructuración del sistema agroalimentario mundial. El caso  en estudio aborda la conformación social de la cadena frutícola localizada en  el norte de la Patagonia argentina, donde estos requerimientos se formalizan,  entre otros, en las buenas prácticas agrícolas, abordadas como dispositivos de control  que interpelan y vuelven complejas las condiciones de reproducción social de  los chacareros, como productores independientes en la cadena, con impactos en  sus trayectorias vitales y productivas. Se consultaron fuentes secundarias, y  se realizó el relevamiento primario de casos.","palabras_clave":"PRODUCTORES FAMILIARES|BUENAS PRÁCTICAS AGRÍCOLAS|TRAYECTORIAS PRODUCTIVAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64450,"idioma_id":4,"titulo":"Moult cycle and growth of the crab Halicarcinus planatus (Brachyura, Hymenosomatidae) in the Beagle Channel, southern tip of South America","resumen":"The crab Halicarcinus planatus is the only hymenosomatid crab that inhabits thesouthern tip of South America and is the only decapod species that reproduces twice ayear in the Beagle Channel. In this article, we study the moult cycle in the field (moultfrequency, analysis of size frequency distribution) and linked it with growth studied inthe laboratory (absolute and percent growth increment, Hiatt function). Hiatt functionswere similar for males and females. Moult frequency was seasonal: in early australspring and in austral summer. In females, the pubertal moult is the terminal moult,whereas males continue moulting after attaining the size of morphometric maturity.Moult increment was highly variable. The relationship between absolute moultincrement and crab size was described by a quadratic function. Percent growthincrement decreased with size and relationships were different for each sex: linear forfemales and quadratic for males. Seven and eight modal groups explained the sizefrequency distributions for females and males from the field, respectively, and revealedthe existence of two cohorts of recruits per year. Further modal analysis was mainlyhampered by the high variability of size increment that could make any moultingindividual fall in its own or one of two following modal groups. The antagonismbetween growth and reproduction was evident in small males. We hypothesize that theterminal pubertal moult is an advantageous feature that allows females to maximizetheir investment in reproduction after their terminal moult, which allows this species tohave two spawnings per year.","palabras_clave":"ECDYSIS|DECAPODA|SUB-ANTARCTIC|TERMINAL MOULT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64451,"idioma_id":5,"titulo":"Bienestar animal en ovinos en establecimientos agropecuarios durante el transporte y en frigoríficos","resumen":"La tendencia mundial del mercado de carnes está orientada a poner mayor énfasis en satisfacer los requerimientos de los consumidores en términos de la calidad del producto. Por lo tanto, hay que revalorizar nuestros productos a través de procesos que aseguren calidad, seguridad y trazabilidad certificada, sustentabilidad de los sistemas y bienestar animal. Se define como bienestar animal el estado de salud mental y físico de un animal en armonía con su medio ambiente. En la presente revisión nos abocaremos a la importancia del bienestar animal sobre la producción primaria, transporte y frigoríficos y los efectos que pueden tener en la cantidad y calidad de productos. Para tal fin se realizó una búsqueda bibliográfica, nacional e internacional y consultas a productores, empresarios de la carne y profesionales. Se concluye que son múltiples y complejos los factores que pueden afectar al sector ovino y que para atenuar sus efectos se debe disponer de conocimientos sobre   historia de la evolución del ovino, características zootécnicas, tología, buenas prácticas de manejo, infraestructura, equipos y personal capacitado en los establecimientos rurales, transporte y frigoríficos.","palabras_clave":"OVINOS|BIENESTAR|TRANSPORTE|FRIGORÍFICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64452,"idioma_id":4,"titulo":"Exploration of the nutritional profile of Trichocereus terscheckii (Parmentier) Britton & Rose stems","resumen":"Columnar cacti Trichocereus terscheckii is an important source of food and water for insects, birds and wild cattle in dry areas of North West Argentina. Their fruits have been suggested as a plausible source of food, but are not directly included in the human diet yet. Stem chemistry has long been of interest because of its hallucinogenic properties, but little is known about the nutritional value. In this work, basic nutritional composition of a mature stem was analyzed establishing moisture, total fat, ash, fiber, protein, lipidic and sugar profile. The main constituents fatty acid found in T. terscheckii were linolenic, linoleic, palmitic and oleic acids, but also a rare isomer of oleic acid was relatively abundant. This pattern did not agree with the profiles found in other species of columnar cacti. Current exploration of nutritional profiles of T. terscheckii stem provides novel information that contributes to a better understanding of this species whose slow development rate, high water storage capacity and hallucinogenic properties are features that make it especially vulnerable to an increased rate of extraction, habitat fragmentation and extensive cattle ranching.","palabras_clave":"ALKALOIDS|CARDON|ECHINOPSIS|FATTY ACIDS|NUTRITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64453,"idioma_id":4,"titulo":"Coupling between proton binding and redox potential in electrochemically active macromolecules. The example of Polyaniline","resumen":"In this work it is investigated the coupling between the redox potential and the extent of proton binding in electrochemically active polymers, for the particular case of Polyaniline (Pani). To this purpose, the degree of oxidation of the polymer was measured by spectrophotometry changing the external potential applied to Pani films, in solutions of different pH values. The knowledge of the oxidation degree for the different applied potentials allows determining the apparent formal redox potential. For Pani, in the hypothetical case of absence of interactions between the redox centres, the apparent formal redox potential should be independent of the oxidation degree and it should decrease linearly with the pH with a slope of 0.059 V, at room temperature. The values of the apparent formal redox potential, experimentally obtained, show that the pH dependence is not as expected from the redox equation. This fact implies interactions between the redox centres and some sort of coupling between the redox potential and the state of binding of the polymer. By employing a simple statistical mechanic model is possible to evaluate the interaction energy between the redox centres and also the acid dissociation constants of both, the oxidized and reduced forms. The values obtained for both dissociation constants agree with some reported in the literature. The application of the model also allows explaining the observed relationship between the apparent formal redox potential and the electrolyte pH.","palabras_clave":"Proton Binding|Conducting Polymers| Redox Potential Distribution|Polyaniline"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64454,"idioma_id":5,"titulo":"Diversidad forestal de un hábitat altamente amenazado en bosques del Chaco Semiárido","resumen":"El bosque chaqueño argentino sufre un acelerado proceso de degradación por dos causas 31 principales: la extracción de maderas duras y la ganadería extensiva, produciendo el reemplazo de la Diversidad forestal de un hábitat altamente amenazado la vegetación original por arbustales de bajo potencial. El objetivo del trabajo es analizar la diversidad, 33 composición y estructura de bosque en 4 sitios en el área del Río Salado, localidad Santos Lugares, Departamneto Alberdi, Santiago del Estero, Argentina, con diferentes historias de aprovechamiento. El estudio se  realizó en: Parque Los Quebrachos (bosque con una clausura de 20 años); Maravilla (bosque 36 secundario degradado con suelos salinos); Ahí Veremos (la masa ha sido intervenida en forma muy 37 intensa, hace 40 años), Buen Lugar (bosque secundario sometido a corta intensiva hace 30 años). Se 38 seleccionaron áreas de interés, mediante imágenes satelitales LANDSAT TM 5, delimitándose 5 has. 39 La muestra es de 500 m, con parcelas de 4* 25 m. Se censaron las especies leñosas y crasas presentes, y el conteo de individuos. Se midieron las siguientes variables: DAP, altura de fuste (H F) y Nº 41 árboles\/ha. Fue aplicado análisis exploratorio multivariado por clúster y componentes principales. Se 42 calculó diversidad alfa, beta y gama. Se contrastaron datos de diversidad y vol. de la masa forestal, 43 presentando correlación positiva.","palabras_clave":"BIODIVERSIDAD|USOS|BOSQUES|CHACO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64455,"idioma_id":5,"titulo":"Primeros fechados radiocarbónicos para el Sector B del sitio Alero Deodoro Roca (Ongamira, Córdoba, Argentina)","resumen":"La arqueología de las Sierras Centrales argentinas fue construida a través de una serie de caracterizaciones cronológicas y contextos arqueológicos asociados construida por el Dr. Alberto Rex González entre las décadas de los años 50 y 60 (González 1960). Para llevar adelante dicha construcción utilizó sitios claves, con secuencias maestras, siendo uno de los principales el Alero Deodoro Roca, situado en el valle de Ongamira, Depto. Ischilín, en el norte de la provincia de Córdoba (Figura 1).   El Alero Deodoro Roca es conocido desde principios del siglo XX, pero no fue hasta la década de 1930 que se centró el interés en las ocupaciones prehispánicas del valle. Aníbal Montes (1943) fue quien comenzó con una serie de excavaciones no sistemáticas donde definió dos sectores dentro del sitio: A y B (Figura 2). La descripción de las primeras excavaciones que comenzaron en el sector A del Alero Deodoro Roca y alcanzaron algunas porciones del sector B, fue publicada en las actas del Congreso de Historia Argentina del Norte y Centro llevado a cabo en la ciudad de Córdoba en el año 1941, evento en el cual también se creó el Instituto de Arqueología, Lingüística y Folklore de la UNC (Bonnín 2008). En ese mismo volumen González (1943) hace una descripción del material hallado en estas excavaciones. De estos trabajos de campo no se cuenta con registros sistemáticos, aunque sí con restos de colecciones que se encuentran en el Museo de Antropología de la FFyH, UNC, y probablemente en otros museos de Argentina.   El interés de Montes en encontrar el hombre fósil que habría habitado este alero, orientó el trabajo de campo a la búsqueda de restos humanos de gran antigüedad. Este interés, sin duda, marcó la metodología de excavación centrada en la remoción de grandes volúmenes de sedimento con el fin de llegar a las partes más profundas de la estratigrafía del sitio y el sondeo de áreas intermedias desde el sector A hasta llegar al sector B. En este sentido durante sus trabajos fueron hallados nueve esqueletos, muchos de los cuales por cuestiones tafonómicas y la metodología de la época no fueron preservados. Sólo algunos cráneos sobrevivieron y fueron llevados por González al American Museum of Natural History (AMNH), lugar en donde se encuentran en la actualidad (Aricanli comunicación personal, González comunicación personal).   En el año 1950, Alberto Rex González junto con Osvaldo Menghin (acompañados por Aníbal Montes), vuelven a Ongamira y plantean una excavación en el Sector B de igual magnitud que la llevada a cabo en el sector A (Bianciotti 2005, González 2008) (Figura 2, Figura 3a).   En esta oportunidad realizan un grillado del sector noreste (B) del sitio de unos 44 metros de ancho (en sentido E-O) y unos 20 metros de profundidad (en sentido N-S). Este grillado se compuso de 220 cuadrículas de 2 metros de lado (Figura 3a). De este reticulado se seleccionaron 31 cuadrículas que fueron excavadas desde la superficie hasta los 2,8 metros de profundidad, aunque algunas superaron esa marca (ver Menghin y González 1954: Figura 5). Entre las cuadriculas seleccionadas se realizó una trinchera siguiendo la línea de goteo del alero lo que les permitió obtener un perfil E-O y otra trinchera para obtener un perfil N-S. Estas dos se cruzaban en la zona central de la acumulación de restos materiales (sector central del cuadriculado). Esto coincidió con la zona de mayor altura de abundancia de restos arqueológicos, que según la descripción de Menghin y González, poseía forma monticular (Menghin y González 1954).  Luego de estas excavaciones que se resumen en la publicación de 1954, Aníbal Montes vuelve a excavar el sector A en los años 1957 y 1958, ampliando las excavaciones de las décadas de 1930 y 1940 (Figura 2, FDAM Caja 1, Folio 6). Allí, excava un área de unos 4 x 4m y unos 6 m de profundidad y una trinchera de unos 25 metros de largo que saliendo del sector anteriormente nombrado avanza en dirección noreste hacia los sectores más bajos del terreno . Algunas falanges de camélidos y otros restos de cultura material que se encuentran en la Reserva Patrimonial del Museo de Antropología (FFyH, UNC) nos ha permitido hacer algunas interpretaciones acerca de las ocupaciones del sector A (Izeta y Bonnin 2009, Cattáneo e Izeta 2011, Costa et al. 2011).","palabras_clave":"CRONOLOGIA|CORDOBA|ARQUEOLOGIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64456,"idioma_id":4,"titulo":"Polyfunctional T Cell Responses in Children in Early Stages of Chronic Trypanosoma cruzi Infection Contrast with Monofunctional Responses of Long-term Infected Adults","resumen":"Abstract      BACKGROUND:       Adults with   chronic Trypanosoma cruzi exhibit a poorly functional T cell   compartment, characterized by monofunctional (IFN-&#947;-only secreting)   parasite-specific T cells and increased levels of terminally   differentiated T cells. It is possible that persistent infection and\/or   sustained exposure to parasites antigens may lead to a progressive loss   of function of the immune T cells.      METHODOLOGY\/PRINCIPAL FINDINGS:       To   test this hypothesis, the quality and magnitude of T. cruzi-specific T   cell responses were evaluated in T. cruzi-infected children and compared   with long-term T. cruzi-infected adults with no evidence of heart   failure. The phenotype of CD4(+) T cells was also assessed in T.   cruzi-infected children and uninfected controls. Simultaneous secretion   of IFN-&#947; and IL-2 measured by ELISPOT assays in response to T. cruzi   antigens was prevalent among T. cruzi-infected children. Flow cytometric   analysis of co-expression profiles of CD4(+) T cells with the ability   to produce IFN-&#947;, TNF-&#945;, or to express the co-stimulatory molecule CD154   in response to T. cruzi showed polyfunctional T cell responses in most   T. cruzi-infected children. Monofunctional T cell responses and an   absence of CD4(+)TNF-&#945;(+)-secreting T cells were observed in T.   cruzi-infected adults. A relatively high degree of activation and   differentiation of CD4(+) T cells was evident in T. cruzi-infected   children.      CONCLUSIONS\/SIGNIFICANCE: Our observations are   compatible with our initial hypothesis that persistent T. cruzi   infection promotes eventual exhaustion of immune system, which might   contribute to disease progression in long-term infected subjects","palabras_clave":"CHAGAS|T. CRUZI|T CELL RESPONSES|PARASITOLOGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64457,"idioma_id":4,"titulo":"Bivalve distribution in hydrographic regions in South America: historical overview and conservation","resumen":"Abstract Based on literature review and malacological collections, 168 native freshwater bivalve and  five invasive species have been recorded for 52  hydrographic regions in South America. The higher  species richness has been detected in the South  Atlantic, Uruguay, Paraguay, and Amazon Brazilian  hydrographic regions. Presence or absence data were  analysed by Principal Coordinate for PhylogenyWeighted. The lineage Veneroida was more representative in hydrographic regions that are poorer in  species and located West of South America. The  Mycetopodidae and Hyriidae lineages were predominant in regions that are richest in species toward the  East of the continent. The distribution of invasive  speciesLimnoperna fortuneiis not related to species  richness in different hydrographic regions there. The  species richness and its distribution patterns are  closely associated with the geological history of the  continent. The hydrographic regions present distinct  phylogenetic and species composition regardless of  the level of richness. Therefore, not only should the  richness be considered to be a criterion for prioritizing  areas for conservation, but also the phylogenetic  diversity of communities engaged in services and  functional aspects relevant to ecosystem maintenance.  A plan to the management of this fauna according to particular ecological characteristics and human uses of  hydrographic regions is needed","palabras_clave":"Bivalve|South America|Literature veview|Phylogenetic composition"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64458,"idioma_id":4,"titulo":"Sedimentary patterns in the Vinchina Basin: interplay between compressional and transcurrent tectonism during the Andean orogeny","resumen":"    The Nevados  del Famatina, located in northwestern Argentina  (Fig. 1), is one of the higher non-volcanic mountains belts in South America reaching up to 6200 m in high. Despite the  fact that the uplift of the Nevados del Famatina was (and is) closely related to  the Andean Orogeny, these mountains are located about 400 km from the Chile trench and up to 100 km from the main Andean Cordillera (Cobbold et al., 2007). The origin of the high  and isolated Nevados del Famatina is related to a Neogene tectonic syntaxis due  to the relationship between the left-lateral Valle Fértil wrenching and the  right-lateral Tucuman wrenching (Rossello et  al., 2011). The Vinchina Basin, a highly subsiding basin that &nbsp;accumulated more than 10.000 meters of  continental sediments during the Neogene developed between the magmatic arc,  located in the Andean Cordillera, and the Famatina mountain. The study of the  sedimentary fill of the Vinchina   Basin allows  reconstructing not only the evolution of the magmatic arc (and its associated deformation)  but also the timing and effect of the uplift of the Nevados del Famatina. The stratigraphy of the VinchinaBasin, which very probably represents  the northern culmination of the Bermejo   Basin, was studied by several  authors. Recently, Ciccioli et al.  (2011) divided the Vinchina   Basin fill in five stages  named: 1. retroarc, 2. early transpressive retroarc, 3. late transpressive  retroarc, 4. transpressional foreland and 5. cannibalized foreland. New  radiometric ages and stratigraphic evidences allow improving the timing of these  stages and relate them with the uplift of the Nevados del Famatina and the  basement blocks of Sierras Pampeanas Noroccidentales.  ","palabras_clave":"Vinchina|Andes|Famatina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64459,"idioma_id":4,"titulo":"Interaction between Estuarine Microphytobenthos and  Physical Forcings: The Role of Atmospheric and  Sedimentary Factors","resumen":"The goal of this study was to analyze microbial mats and biofilms from the lower supratidal area of the Bahía Blanca estuary (Argentina), and explore their relationship with sediments and other physical forcings. Thirteen monthly sediment samples (uppermost 10 mm) were taken and their composition and abundance in microorganisms was determined by microscopy. Physical parameters (solar radiation and sediment temperature at &#8722;5 cm) were recorded with a frequency of 5 minutes by a coastal environmental monitoring station. Additionally, sediment grain size and moisture content were determined for distinct layers in the uppermost 20 mm, and the rate of inundation of the supratidal area was estimated from tidal gauge measurements. There were significant seasonal differences in the biomass of the microphytobenthic groups considered (filamentous cyanobacteria and epipelic diatoms), with the former consistently making up >70% of the total biomass. The relationships between microphytobenthos and sediment temperature and solar radiation fitted to linear regressions, and consistently showed an inverse relationship between microphytobenthic abundance and either one of the physical parameters. The granulometric analysis revealed a unimodal composition of muddy sediments, which were vertically and spatially homogeneous; additionally, there were significant seasonal differences in water content loss with drying conditions prevailing in the summer. Several Microbially-Induced Sedimentary Structures (MISS) were identified in the supratidal zone such as shrinkage cracks, erosional pockets, gas domes, photosynthetic domes, mat chips and sieve-like surfaces. In contrast to studies from analogous environments in the Northern Hemisphere, we found reduced microphytobenthic biomass in summer, which were explained by increased evaporation\/desiccation rates as a consequence of increased radiation, despite frequent tidal inundation. In conclusion, the observed density shifts in the benthic microbial communities are attributable to physical forcings dependent upon seasonal variations in interplaying factors such as sediment temperature, solar radiation and tidal inundation.-","palabras_clave":"BIOFILMS|MICROBIAL MATS|TIDAL FLAT|SOLAR RADIATION|SEDIMENT GRAIN SIZE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64460,"idioma_id":5,"titulo":"Componentes del balance de calor estival en la playa de Pehuén Co (Argentina)","resumen":"El objetivo del presente trabajo fue el cálculo del balance de calor de la  zona costera de Pehuén-Co y el análisis de la importancia del viento y la marea sobre los flujos calóricos. Los resultados se comparan con estudios similares de playas con el objeto de establecer patrones de intercambio energético en estos ambientes. Este estudio forma parte de las primeras etapas de una investigación más amplia de la playa de Pehuén-Co, tendiente a establecer relaciones entre la temperatura y biodiversidad del ecosistema costero.","palabras_clave":"balance de calor|Pehuen Co|playas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64461,"idioma_id":5,"titulo":"Evaluación de estrategias de vacunación contra la tos convulsa  mediante un modelo matemático de transmisión de la enfermedad","resumen":"La tos convulsa o  pertussis es una enfermedad respiratoria inmunoprevenible que ha resurgido en  las últimas décadas. La mayor morbi-mortalidad se registra en los lactantes  aunque también se detectan casos en adolescentes y adultos. La situación  epidemiológica de la enfermedad ha obligado a revisar e implementar nuevas  estrategias para mejorar su control. Muchas de estas estrategias, sin embargo,  aún no cuentan con sustento experimental que permita su universalización. En  este contexto los modelos matemáticos de transmisión de enfermedades resultan  herramientas útiles en la toma de decisiones. En este trabajo evaluamos,  mediante un modelo matemático para pertussis, el impacto que tendrían distintas  medidas de control en la población más vulnerable (0-1 año). En particular se  analizó el impacto de la inclusión de un refuerzo a los 11 años, el efecto de la  mejora en las coberturas de las dosis primarias y la disminución del retraso en  la aplicación de mismas. También se estimó el efecto de la vacunación a  embarazadas. Nuestros resultados muestran que la inclusión del refuerzo de los  11 años disminuye en un 3%la incidencia  en los menores de 1 año. Por su parte la aplicación de las dosis primarias a  tiempo calendario (sin retrasos) la reduce en 16%. Al aumentar la cobertura del  80% al 95%, la incidencia en la población vulnerable se reduce  significativamente (38%). Cuando el porcentaje de las embarazadas inmunizadas  alcanza el 50%, la reducción de los casos más graves en infantes superaría el  33%.El artículo también está en idioma inglés en http:\/\/www.sap.org.ar\/docs\/publicaciones\/archivosarg\/2013\/v111n5a03e.pdf&nbsp;&nbsp; ","palabras_clave":"TOS CONVULSA|BORDETELLA PERTUSSIS|VACUNA ANTI PERTUSSIS|INMUNIZACION|REFUERZO|MODELO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64462,"idioma_id":4,"titulo":"Dispersal of Aedes aegypti: field study in temperate areas using a novel method","resumen":"Background &amp; objectives: Since Aedes aegypti was identified as vector of yellow fever and dengue, its dispersalis relevant for disease control. We studied the dispersal of Ae. aegypti in temperate areas of Argentina during egglaying,using the existing population and egg traps.Methods: Two independent replicas of a unique experimental design involving mosquitoes dispersing from anurbanized area to adjacent non-urbanized locations were carried out and analyzed in statistical terms.Results: We found relationship between stochastic variables related to the egg-laying mosquito activity (ELMA),useful to assess dispersal probabilities, despite the lack of knowledge of the total number of ovipositions in thezone. We propose to evaluate the egg-laying activity as minus the logarithm of the fraction of negative ovitraps atdifferent distances from the buildings.Interpretation &amp; conclusion: Three zones with different oviposition activity were determined, a corridor surroundingthe urbanization, a second region between 10 and 25 m and the third region extending from 30 to 45 m from theurbanization. The landscape (plant cover) and the human activity in the area appear to have an influence in thedispersal of Ae. aegypti. The proposed method worked consistently in two different replicas.","palabras_clave":"Aedes aegypti|dengue|dispersal|multinomial analysis|oviposition|yellow fever"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64463,"idioma_id":4,"titulo":"A novel method of rejection of brood parasitic eggs reduces parasitism intensity in a cowbird host","resumen":"The hosts of brood parasitic birds are under strong selection pressure to recognize and remove foreign eggs from their nests, but parasite eggs may be too large to be grasped whole and too strong to be readily pierced by the host´s bill. Such operating constraints on egg removal are proposed to force some hosts to accept parasite eggs, as the costs of deserting parasitized clutches can outweigh the cost of rearing parasites. By fitting microcameras inside nests, we reveal that the Neotropical baywing (Agelaioides badius), a host of the screaming cowbird (Molothrus rufoaxillaris) and shiny cowbird (Molothrus bonariensis), instead circumvents such constraints by kicking parasite eggs out of the nest. To our knowledge, this is the first report of a passerine bird using its feet to remove objects from the nest. Kick-ejection was an all-or-nothing response. Baywings kick-ejected parasite eggs laid before their own first egg and, if heavily parasitized, they ejected entire clutches and began again in the same nest. Few baywings were able to rid their nests of every parasite egg, but their novel ejection method allowed them to reduce the median parasitism intensity by 75 per cent (from four to one cowbird eggs per nest), providing an effective anti-parasite defence.","palabras_clave":"Brood parasitism|Antiparasitic defense|Agelaioides badius|Baywing"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64464,"idioma_id":4,"titulo":"High rates of Shiny Cowbird parasitism on Brown-and-yellow Marshbird select for complementary host defenses","resumen":"Hosts of brood parasites may have not developed antiparasitic defenses either because they are recently sympatric or because costs of potential defenses outweigh their benefits. We studied antiparasitic defenses of Brown-and-yellow Marshbird (Pseudoleistes virescens) against Shiny Cowbird (Molothrus bonariensis). We evaluated recognition and aggression toward female and male Shiny Cowbirds, estimated rejection rates of cowbird eggs of different colors, and tested the effect of parasite egg size on host rejection. We also observed and video-recorded host nests to estimate host nest attentiveness, frequency of cowbird visits and interactions between marshbirds and cowbirds. When marshbirds were faced with dummy models, they attacked first and more intensively those of female and male cowbirds than those of a control species. Frequency of egg ejection increased as differences between cowbird and spotted host eggs increased (immaculate>intermediate>spotted)  and spotted eggs were ejected more frequently when laid before rather than during or after host laying. Marshbirds ejected artificially added immaculate eggs independently of their size. Shiny Cowbirds only visited marshbird nests at the egg stage. Host nest attention was low during egg laying and increased during incubation and after hatching, but aggressiveness against cowbirds was always high. However, nest defense was inefficient as losses due to cowbird egg pecking were high. Ejection of cowbird eggs prevented the cost of lower  survival of marshbird nestlings in highly-parasitized nests. As this defense is cost-free, this small benefit would be sufficient to select for the evolutionary maintenance of egg ejection.","palabras_clave":"Brood parasitism|antiparasitic defenses|Pseudoleistes virescens|Molothrus bonariensis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64465,"idioma_id":4,"titulo":"Multilevel Current Source Inverter with FPGA Control","resumen":"In this article a multilevel current source inverter (MCSI) topology is analyzed. The issue of constructing a novel modular single-rating inductor MCSI is explored, taking advantage of the features of Field Programmable Gate Array devices (FPGA) for control and gate signals generation. The proposed topology is built with identical modules where all inductors carry the same amount of current, simplifying the construction and operation of industrial applications with higher efficiency. A new state machine approach, easy to implement in a FPGA, and a proper implementation of the Phase-Shifted Carrier Sinusoidal Pulse Width Modulation (PSC-SPWM) allow both current balance in all modules and effective switching frequency minimization. The performance of the MSCI proposed is simulated with Matlab and verified by constructing a prototype.","palabras_clave":"Field-programmable gate array (FPGA)|multilevel current-source inverter (MCSI)|phase-shifted carrier SPWM (PSC-SPWM)"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64466,"idioma_id":4,"titulo":"Anisotropic halo model: implementation and numerical results","resumen":"In the present work, we extend the classic halo model for the  large-scale matter distribution including a triaxial model for the halo  profiles and their alignments. In particular, we derive general  expressions for the halo-matter cross-correlation function. In addition,  by numerical integration, we obtain instances of the cross-correlation  function depending on the directions given by halo shape axes. These  functions are called anisotropic cross-correlations. With the aim of  comparing our theoretical results with the simulations, we compute  averaged anisotropic correlations in cones with their symmetry axis  along each shape direction of the centre halo. From these comparisons we  characterize and quantify the alignment of dark matter haloes on the  &#923;cold dark matter context by means of the presented anisotropic  halo model. Since our model requires multidimensional integral  computation we implement a Monte Carlo method on GPU hardware which  allows us to increase the precision of the results and it improves the  performance of the computation.","palabras_clave":"methods: numerical|methods: statistical,|dark matter|large-scale structure of Universe"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64467,"idioma_id":4,"titulo":"The unknown larva of the minute diving beetle genus Brachyvatus Zimmermann, 1919 (Coleoptera: Dytiscidae: Bidessini)","resumen":"Thethree larval instars of Brachyvatusacuminatus (Steinheil, 1869) (the type species of the bidessine genus Brachyvatus Zimmermann, 1919) aredescribed and illustrated for the first time including detailed morphometricand chaetotaxic analyses of the cephalic capsule, head appendages, legs, lastabdominal segment and urogomphi. Larvae of this genus are characterized by theabsence of the primary pore ABc, which is a synapomorphy of the tribeBidessini. Brachyvatus larvae can beseparated from those of all other known Bidessini genera by the followingcombination of characters: very small size; lateral branches of nasale wellvisible in dorsal view; ventroapical spinula on third antennomere absent; meso-and metatergite of instar I with anterotransverse carina; siphon short; longurogomphi with secondary setae; seta PA3 absent; setae UR2 and UR4 insertedcontiguously; natatory setae present on tibiae in instars II-III.","palabras_clave":"HYDROPORINAE|BRACHYVATUS ACUMINATUS|LARVAE|MORPHOMETRY|CHAETOTAXY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64468,"idioma_id":4,"titulo":"Aquatic Coleoptera from two protected areas of the Humid Chaco eco-region (Chaco Province, Argentina)","resumen":"Alist of the species of aquatic Coleoptera inhabiting Chaco National Park and ElCachapé Wildlife Refuge, two protected areas located in the Humid Chacoeco-region, is presented for the first time. One hundred and twenty-two speciesincluded in 45 genera and 10 families were identified. Two species are new forArgentina: Oraatroapicalis Pic and Ora semibrunnea Pic (Scirtidae). Tengenera (Dytiscidae: Anodocheilus Babington, Bidessonotus Régimbart, HemibidessusZimmermann; Noteridae: Mesonoterus Sharp, Notomicrus Sharp;Hydraenidae: Hydraena Kugelann, Gymnochthebius Orchymont; Hydrophilidae: Chasmogenus Sharp, Chaetarthria Stephens; Scirtidae: Ora Clark, ScirtesIlliger)and 41 species are first cited from Chaco Province. The high diversity ofaquatic Coleoptera recorded highlights the importance of these protected areas.","palabras_clave":"AQUATIC COLEOPTERA|INVENTORY|CHACO NATIONAL PARK|EL CACHAPÉ REFUGE|NEOTROPICAL REGION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64469,"idioma_id":4,"titulo":"Land use effects on soil carbon in the Argentine Pampas","resumen":"Our objective was to establish the pattern of variation of soil organic (SOC) and inorganic (SIC) carbon stored in surface and deep soil layers of the Argentine Pampas as affected by environmental conditions and land use. Eighty two farms, widespread over the region, were used for the study. At each farm paired treatments were sampled representing common land uses: trees, uncropped controls, seeded pastures, cropped fields and periodically flooded areas. Bulk density, SOC, SIC, texture, pH and electrical conductivity were determined to1 m depth. Rainfall and temperature were obtained from climatic records. Significant differences were detected between treatments in SOC contents. Average SOC stocks to 1 m were: 131 t ha&#8722;1 under trees&gt;101 t ha&#8722;1 in uncropped control&gt;90 t ha&#8722;1 in pastures=86 t ha&#8722;1 in cropped field&gt;and 70 t ha&#8722;1 in flooded sites. Compared with uncropped controls, SOC was significantly different in all soil layers under trees, to 75 cm depth in flooded sites and to 50 cm in pastures and cropped soils. Agriculture determined a reduction of 16% of SOC to 50 cm in sampled sites. In the 50\u2013100 cmdepth a decrease of 9%was observed, though not significant. The stratification pattern of SOC in depth was not affected by the treatments; implying that land use impacted the SOC sequestered in soil, but not its allocation in depth. SIC accounted for one third of total soil carbon, average SICstockwas 50 t C ha&#8722;1 to 1 m. Both, its stock and distribution in the profile were not affected by the treatments; with greater SIC stocks founded in deep soil layers. An artificial neural network model was developed that allowed the estimation of SOC (R2=0.64) based on climate, soil properties and land use. The model, linked to information from satellite image classification, was used for the estimation of present SOC stock of pampean soils, which accounted for 4.22±0.14 Gt in an area of 48.2 Mha. Using soil surveys performed during the1960\u20131980 period we estimated a SOC stock of 3.96±0.22 Gt. Consequently, no change of total SOC stock seems to be produced in the last decades in the region. At smaller scale, counties with SOC content greater than 95 t ha&#8722;1 to 1 m depth lost carbon; increases prevailed below this threshold. Apparently, SIC reservoirs seem have not change during the last decades.","palabras_clave":"Carbon sequestration|Soil organic carbon|Soil inorganic carbon |Empirical modeling"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64470,"idioma_id":4,"titulo":"Emulsion Polymerization of MMA employing a Chain Transfer Agent of Low Environmental Impact: Iso-Octyl-3- Mercaptopropionate","resumen":"The unseeded batch-emulsion polymerisation of methyl methacrylate (MMA) was investigated using the chain transfer agent iso-octyl-3-mercaptopropionate (iOMP), which exhibits a low environmental impact. The commercial iOMP is a mixture of over 10 isomers that proved adequate to control the molecular weights of poly(methyl methacrylate) with a decreasing evolution of both the number- and weight-average molecular weights along the reactions. The iOMP also affects the polymerisation kinetics as a consequence of the lower reactivity of the iOMP radicals with respect to the MMA-ended radicals. Experimental results were interpreted with the help of a first principles mathematical model.","palabras_clave":"POLY(METHYL METHACRYLATE)|MOLECULAR WEIGHT DISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64471,"idioma_id":4,"titulo":"Synthesis of Latex-Antigen Complexes from Single and Multiepitope Recombinant Proteins. Application in Immunoagglutination Assays for the Diagnosis of Trypanosoma cruzi Infection","resumen":"This article investigates the physical adsorption and the chemical coupling of three different recombinant proteins of Trypanosoma cruzi, not previously investigated for developing agglutination tests, onto sub-micrometric core-shell carboxylated particles to be used in an immunoassay for detecting the Chagas disease. The coupled recombinant proteins were the multiepitope protein CP2 (derived from three antigenic peptides) and two single proteins (RP1 and RP5). In all cases, high fractions of proteins chemically-bound to carboxyl groups were observed and the highest surface densities of linked proteins were attained at pH values close to the proteins isoelectric point. The latex-CP2 complex obtained by covalent coupling produced better results than both the latex-RP1 and the latex-RP5 complexes in the immunoagglutination assays, i.e., higher discrimination between sera from a panel of 16 infected and 16 non-infected patients.","palabras_clave":"LATEX PROTEIN COMPLEX|IMMUNOASSAY|CHAGAS DESEASE|MULTIEPITOPE PROTEIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64472,"idioma_id":4,"titulo":"Mass dependence of the deconfinement and chiral restoration critical temperatures in nonlocal SU(2) PNJL models","resumen":"In the framework of nonlocal SU(2) chiral quark models with Polyakov loop, we analyze thedependence of the deconfinement and chiral restoration critical temperatures on the explicit chiralsymmetry breaking driven by the current quark mass. Our results are compared with those obtained within the standard local Polyakov-Nambu-Jona-Lasinio model and with lattice QCD calculations. For a wide range of pion masses, it is found that both deconfinement and chiral restoration critical temperatures turn out to be strongly entangled, in contrast with the corresponding results within the Polyakov-Nambu-Jona-Lasinio model. In addition, it is seen that the growth of the critical temperatures with the pion mass above the physical point is basically linear, with a slope parameter that is close to the existing lattice QCD estimates. On the other hand, we find a tendency to favor an early onset of the first order transition expected in the large quark mass limit.","palabras_clave":"non-local models|deconfinement|chiral restoration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64473,"idioma_id":4,"titulo":"Predictive Contribution of Personality Traits in a Socio-Cognitive Model of Academic Performance in Mathematics","resumen":"Social Cognitive Career Theory (SCCT) explains academic performance as a function of conceptually distinct and interrelated cognitive variables. We aimed at extending SCCT?s performance model by examining the direct and indirect ? through socio-cognitive variables - contribution of personality traits on mathematics academic performance. Argentine youths (N = 543) were assessed in mathematics self-efficacy, outcome expectations, performance goals and skills. Personality traits were assessed through the Big Five Questionnaire for Children. Path analyses indicated that the contribution of personality on academic performance was mediated by socio-cognitive mechanisms. The results suggested that (a) students who exhibited higher Conscientiousness scores had higher self-efficacy beliefs and more positive outcome expectations and set more demanding performance goals, and (b) students who had higher Openness\/Intellect scores exhibited higher self-efficacy beliefs. Gender did not moderate the relations among cognitive variables but influenced the association between personality traits and cognitive variables. Implications for teachers and guidance counselors are discussed.","palabras_clave":"Self-efficacy|Personality Traits|Performance Goals|Social Cognitive Career Theory"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64474,"idioma_id":4,"titulo":"Bottom-Up Analysis of Energy Consumption and Carbon Emissions, with Particular Emphasis on Human Capital Investment","resumen":"Short-term and mid-term projections of energy consumption and carbon emissions raise significant concern about the availability of the necessary energy resources to meet the growing demand and about the impact of emissions on global change. Different macroeconomic models address this issue through global variables, such as gross domestic product, production of goods and services, total population and natural resources extraction. However, the relations among these variables are neither linear nor simple. In an attempt to base said relations on a ?bottom-up? perspective, the individual behavior of representative agents of economy, in terms of energy consumption and related carbon emissions, was stud-ied, with particular emphasis on their investment in human capital. It was found that a higher investment in human capital (e.g., education, research) was translated into a better distribution of consumption, with a higher level of energy efficiency and a slight improvement in carbon emissions intensity","palabras_clave":"CARBON EMISSIONS|ECONOMIC GROWTH|HUMAN CAPITAL|ENERGY INTENSITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64475,"idioma_id":5,"titulo":"MODELADO DE LA ÚLTIMA ERUPCIÓN VOLCÁNICA DEL COMPLEJO VOLCÁNICO PUYEHUE-CORDÓN CAULLE","resumen":"El presente trabajo está centrado en la utilización y validación del modelo Euleriano FALL3D para la simulación de la dispersión de aerosoles, durante la erupción del complejo volcánico Puyehue-Cordón Caulle, ocurrida el 4 de junio de 2011 a las 14:45 LT (18:45 UTC). Este modelo fue configurado para modelar la pluma de cenizas durante un período de 10 días a lo largo de 1200 km de extensión territorial. FALL3D obtuvo sus condiciones meteorológicas del modelo de mesoescala Weather Research and Forecasting (WRF). El empleo de un algoritmo para la detección de ceniza volcánica sobre imágenes satelitales, verificó la evolución de la pluma señalada por el modelo de dispersión y transporte. El buen acuerdo entre las comparaciones cuantitativas entre las simulaciones con las mediciones sugiere una adecuada aproximación de modelado y permite la aplicación futura de estas herramientas para el estudio de los efectos radiativos de aerosoles volcánicos","palabras_clave":"MODELO FALL-3D|DETECIÓN DE CENIZA|MOELO DE MESOESCALA WRF|COMPLEJO VOLCÁNICO PUYEHUE-CORDÓN CAULLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64476,"idioma_id":5,"titulo":"Categorías urbanísticas y climáticas integradas para estudios microclimáticos del  confort térmico urbano","resumen":"El objeto de este estudio es adoptar y proponer una clasificación en la microescala más detallada de las condiciones urbanas intra-ciudad, en vistas a una optimización del confort higrotérmico, teniendo en cuenta la influencia que pudiera ejercer el tipo de uso del suelo, la forestación y las estructuras urbanas construidas. Se aplican para ello las Áreas Muestras de Estudio para el Área Metropolitana de San Juan, en el marco del sistema de clasificación internacional de Zonas Climáticas Locales. Se actualizaron las áreas seleccionadas en trabajos anteriores usando las Bandas Urbanas Características. Esta nueva selección de puntos de muestreo y clasificación climática urbana, será en posterior trabajo objeto de mediciones y estudios meteorológicos y de calidad del aire especifícos, que permitan evaluar la influencia de la forestación de los Canales Viales Urbanos (CVU) en la mitigación de algunos problemas ambientales producidos por la Isla de Calor. El trabajo logra integrar conceptos propios de la espacialización urbanística de la ciudad de San Juan con criterios de clasificación climática normalizados. Se espera que esta clasificación sea suficientemente general como para permitir comparaciones con áreas similares de distintas ciudades del mundo","palabras_clave":"CLIMA URBANO|ZONAS CLIMÁTICAS URBANAS|CONFORT CLIMÁTICO|CANAL VIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64477,"idioma_id":5,"titulo":"Estimación de las emisiones de carbono a la atmósfera a partir de modelado basado en agentes","resumen":"A nivel global, los niveles crecientes de consumo de energía proveniente de fuentes fósiles y sus consecuentes emisiones de carbono hacia la atmósfera, están incrementando el efecto invernadero adicional, trayendo consecuencias observables sobre el sistema climático. La dinámica poblacional y el crecimiento económico influyen directamente sobre el consumo de bienes, energía primaria, y por consiguiente, sobre dichas emisiones. A partir del Modelado Basado en Agentes (MBA), se construyó un modelo que simula la evolución de las emisiones de carbono teniendo en cuenta el comportamiento individual de los agentes constitutivos de la economía. La utilización de MBA permitió describir el sistema como el resultado de la interacción de individuos, incluyendo variabilidad en los parámetros y el concepto de elección de comportamiento. Se obtuvieron las curvas correspondientes a la evolución temporal del PBI\/cápita, del crecimiento demográfico, del consumo de energía primaria y de las emisiones de carbono, como así también, de la acumulación de este último en la atmósfera. Los resultados obtenidos están en buen acuerdo con los datos históricos y con las proyecciones realizadas por diversas agencias internacionales","palabras_clave":"MODELO BASADO EN AGENTES|EMISIONES DE CO2|DINÁMICA POBLACIONAL|CARBONO ATMOSFÉRICO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64478,"idioma_id":4,"titulo":"Simulating silver iodine dispersion with wrf: hail suppression system analysis","resumen":"The aim of this article is to study silver iodine (AgI) dispersion in Mendoza using the Weather Research and Forcasting model (WRF). AgI is used to seed clouds in hail suppression systems. In particular, in Mendoza, cloud seeding is done through ground generators located in the western part of the province, were most storm cells form. The efficiency of this hail suppression system has not been scientifically proven. This paper provides a methodology to include AgI sources in WRF in order to study its dispersion in the atmosphere. Because of the physical characteristics of ground generators, they are modeled as area sources using the Fire module of WRF. This module has been design to simulate biomass burning. We show how to simulate AgI generators through definition of a special fuel behavior model to use with WRF-Fire. Through dispersion modeling it is possible to see if AgI reaches storm cells. Furthermore, with simulation results it is possible to choose the best location for ground AgI generators. This paper shows a first approach to simulation of hail suppression system currently working in Mendoza","palabras_clave":"HAIL STORM|WRF-FIRE MODEL|SILVER IODIDE|FLARE DISPERSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64479,"idioma_id":4,"titulo":"Epigenetic patterns newly established after interspecific hybridization in natural populations of Solanum","resumen":"Interspecific hybridization is known for triggering genetic and epigenetic  changes, such as modifications on DNA methylation patterns and impact on  phenotypic plasticity and ecological adaptation. Wild potatoes (Solanum,  section Petota) are adapted to multiple habitats along the Andes, and natural  hybridizations have proven to be a common feature among species of this  group. Solanum 9 rechei, a recently formed hybrid that grows sympatrically  with the parental species S. kurtzianum and S. microdontum, represents an ideal  model for studying the ecologically and evolutionary importance of hybridization  in generating of epigenetic variability. Genetic and epigenetic variability  and their correlation with morphological variation were investigated in wild  and ex situ conserved populations of these three wild potato species using  amplified fragment length polymorphism (AFLP) and methylation-sensitive  amplified polymorphism (MSAP) techniques. We observed that novel methylation  patterns doubled the number of novel genetic patterns in the hybrid and  that the morphological variability measured on 30 characters had a higher correlation  with the epigenetic than with the genetic variability. Statistical comparison  of methylation levels suggested that the interspecific hybridization induces  genome demethylation in the hybrids. A Bayesian analysis of the genetic data  reveled the hybrid nature of S. 9 rechei, with genotypes displaying high levels  of admixture with the parental species, while the epigenetic information  assigned S. 9 rechei to its own cluster with low admixture. These findings suggested  that after the hybridization event, a novel epigenetic pattern was rapidly  established, which might influence the phenotypic plasticity and adaptation of  the hybrid to new environments.","palabras_clave":"DNA METHYLATION|HYBRIDIZATION|WILD POTATOES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64480,"idioma_id":4,"titulo":"Embryology of the spider crabs Leurocyclus tuberculosus (H. Milne-Edwards & Lucas 1842) and Libinia spinosa (H. Milne-Edwards 1834) (Brachyura, Majoidea)","resumen":"The embryonic development of the spider crabs Leurocyclus tuberculosus and Libinia spinosa was divided into five periods  based on the differentiation of: I) cleavage, II) embryonic primordium, III) optic lobes, IV) optic lobes pigmented and  V) chromatophores presence. Different traits such as spines, setae and telson morphology distinguish the two species from  period III until hatching. Egg volume was greater in Leurocyclus tuberculosus than in Libinia spinosa. The duration of  each period was different during development. Whereas in Leurocyclus tuberculosus period II (morphogenesis) is the longest,  in Libinia spinosa the period IV is the longest. Complete embryonic development at 14°C lasted 36.7 ± 3.1 days in  Leurocyclus tuberculosus and 57.4 ± 4.4 days in Libinia spinosa.","palabras_clave":"Embryonic development,| Leurocyclus, |Libinia, |Majoidea"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64481,"idioma_id":4,"titulo":"Numerical solution of the dip coating process with insoluble surfactants: Langmuir or a linear approximation for surface tension?","resumen":"In this  work  the  dip  coating  process when  an  insoluble  surfactant  is  adsorbed  at  the  interface  is  numerically  studied. The  work  centers  in the applicability of the linear approximation usually employed  to  model  the  relationship  between  surface tension  and  the  concentration  of  the  surface  active agent.  Reported solutions show that film thicknesses predicted with a linear model can differ significantly from  those  computed  with  a  more  reliable  equation of state.","palabras_clave":"Dip coating|Surfactant effects|Numerical analysis|Finite Element Methods"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64482,"idioma_id":4,"titulo":"Development of an ionic liquid based dispersive liquid-liquid microextraction method for the determination of nifurtimox and benznidazole in human plasma.","resumen":"  Dispersiveionicliquid?liquidmicroextractioncombinedwithliquidchromatographyandUVdetection was usedforthedeterminationoftwoantichagasicdrugsinhumanplasma:nifurtimoxand benznidazole.Theeffectsofexperimentalparametersonextractionefficiency?the typeandvolume of ionicliquidanddispersersolvent,pH,natureandconcentrationofsalt,andthetimefor centrifugationandextraction?were investigatedandoptimized.Matrixeffectsweredetectedand thus thestandardadditionmethodwasusedforquantification.Thismicroextractionprocedureyielded significantimprovementsoverthosepreviouslyreportedintheliteratureandhasseveraladvantages, includinghighinter-dayreproducibility(relativestandarddeviation¼1.02%and3.66%fornifurtimox and benznidazole,respectively),extremelylowdetectionlimits(15.7ngmL1 and 26.5ngmL1 for nifurtimoxandbenznidazole,respectively),andminimalamountsofsampleandextractionsolvent required.Recoverieswerehigh(98.0%and79.8%fornifurtimoxandbenznidazole,respectively). The proposedmethodologyofferstheadvantageofhighlysatisfactoryperformanceinadditiontobeing inexpensive,simple,andfastintheextractionandpreconcentrationoftheseantichagasicdrugsfrom human-plasmasamples,withthesecharacteristicsbeingconsistentwiththepracticabilityrequire- ments incurrentclinicalresearchorwithinthecontextoftherapeuticmonitoring. ?the typeandvolume of ionicliquidanddispersersolvent,pH,natureandconcentrationofsalt,andthetimefor centrifugationandextraction?were investigatedandoptimized.Matrixeffectsweredetectedand thus thestandardadditionmethodwasusedforquantification.Thismicroextractionprocedureyielded significantimprovementsoverthosepreviouslyreportedintheliteratureandhasseveraladvantages, includinghighinter-dayreproducibility(relativestandarddeviation¼1.02%and3.66%fornifurtimox and benznidazole,respectively),extremelylowdetectionlimits(15.7ngmL1 and 26.5ngmL1 for nifurtimoxandbenznidazole,respectively),andminimalamountsofsampleandextractionsolvent required.Recoverieswerehigh(98.0%and79.8%fornifurtimoxandbenznidazole,respectively). The proposedmethodologyofferstheadvantageofhighlysatisfactoryperformanceinadditiontobeing inexpensive,simple,andfastintheextractionandpreconcentrationoftheseantichagasicdrugsfrom human-plasmasamples,withthesecharacteristicsbeingconsistentwiththepracticabilityrequire- ments incurrentclinicalresearchorwithinthecontextoftherapeuticmonitoring. ","palabras_clave":"nifurtimox|benznidazol|extraction|ionic liquids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64483,"idioma_id":4,"titulo":"Angular tricyclic benzofurans and related natural products of fungal origin. Isolation, biological activity and synthesis","resumen":"Naturally-occurring angular tricyclic benzofuran\/isobenzofuran derivatives of fungal origin and related compounds, in which two heterocyclic rings are fused to a central benzenoid moiety, are covered. Emphasis is placed on the structure of the compounds, together with their relevant biological activities, source microorganisms, country or region of origin and environmental conditions. In addition, proposed biosynthetic pathways, as well as the total syntheses of some of the compounds, including those that lead to structural revision or to correct stereochemical assignments, and related synthetic efforts, are discussed in detail.","palabras_clave":"ISOLATION|SYNTHESIS|NATURAL PRODUCTS|TRICYCLIC BENZOFURANS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64484,"idioma_id":4,"titulo":"Male hypogonadism: an extended classification based on a developmental, endocrine physiology-based approach","resumen":"Normal testicular physiology results from the integrated function of the tubular and interstitial compartments. Serum markers of interstitial tissue function are testosterone and insulin-like factor 3 (INSL3), whereas tubular function can be assessed by sperm count, morphology and motility, and serum anti-Mu¨llerian hormone (AMH) and inhibin B. The classical definition of male hypogonadism refers to testicular failure associated with androgen deficiency, without considering potential deficiencies in germ and Sertoli cells. Furthermore, the classical definition does not consider the fact that low basal serum testosterone cannot be equated to hypogonadism in childhood, because Leydig cells are normally quiescent. A broader clinical definition of hypogonadism that could be applied to male patients in different periods of life requires a comprehensive consideration of the physiology of the hypothalamic-  pituitary-testicular axis and its disturbances along development. Here we propose an extended classification of male hypogonadism based on the pathophysiology of the hypothalamic-pituitary-testicular axis in  different periods of life. The clinical and  biochemical features of male hypogonadism vary according to the following: (i) the level of the hypothalamic-pituitary-testicular axis  primarily affected: central, primary or combined; (ii) the testicular cell population initially impaired: whole testis dysfunction or dissociated testicular dysfunction, and: (iii) the period of life when the gonadal function begins to fail: foetal-onset or postnatal-onset.  The evaluation of basal testicular function in infancy and childhood relies mainly on the assessment of Sertoli cell markers (AMH  and inhibin B). Hypergonadotropism should not be considered a sine qua non condition for the diagnosis of primary hypogonadism  in childhood. Finally, the lack of elevation of gonadotropins in adolescents or adults with primary gonadal failure is indicative of a  combined hypogonadism involving the gonads and the hypothalamic-pituitary axis.","palabras_clave":"cryptorchidism|gonadotropins|sex differentiation|puberty"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64485,"idioma_id":4,"titulo":"Heterozygous IGFALS Gene Variants in Idiopathic Short Stature and Normal Children: Impact on Height and the IGF System","resumen":"AbstractBackground: In acid-labile subunit (ALS)-deficient families,heterozygous carriers of IGFALS gene mutations are frequentlyshorter than their wild-type relatives, suggestingthat IGFALS haploinsufficiency could result in short stature.We have characterized IGFALS gene variants in idiopathicshort stature (ISS) and in normal children, determining theirimpact on height and the IGF system. Patients and Methods:In 188 normal and 79 ISS children levels of IGF-1, IGFBP-3,ALS, ternary complex formation (TCF) and IGFALS gene sequencewere determined. Results: In sum, 9 nonsynonymousor frameshift IGFALS variants (E35Gfs * 17, G83S, L97F,R277H, P287L, A330D, R493H, A546V and R548W) werefound in 10 ISS children and 6 variants (G170S, V239M,N276S, R277H, G506R and R548W) were found in 7 normalchildren. If ISS children were classified according to the abilityfor TCF enhanced by the addition of rhIGFBP-3 (TCF+), carriers of pathogenic IGFALS gene variants were shorter andpresented lower levels of IGF-1, IGFBP-3 and ALS in comparisonto carriers of benign variants. In ISS families, subjectscarrying pathogenic variants were shorter and presentedlower IGF-1, IGFBP-3 and ALS levels than noncarriers. Conclusions:These findings suggest that heterozygous IGFALSgene variants could be responsible for short stature in a subsetof ISS children with diminished levels of IGF-1, IGFBP-3and ALS.","palabras_clave":"Acid-labile subunit    |IGFALS gene|Genetic endocrine disorders|Idiopathic short stature|IGFBP-3|IGF-I"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64486,"idioma_id":4,"titulo":"Male Central Precocious Puberty: Serum Profile of Anti-Müllerian Hormone and Inhibin B before, during, and after Treatment with GnRH Analogue","resumen":"We aimed to describe the functional changes of Sertoli cells, based on the measurement of serum anti-Müllerian hormone (AMH) and inhibin B during treatment with GnRHa and after its withdrawal in boys with central precocious puberty. Six boys aged 0.8 to 5.5&#8201;yr were included. AMH was low at diagnosis in patients >1&#8201;yr but within the normal range in younger patients. AMH increased to normal prepubertal levels during treatment. After GnRHa withdrawal, AMH declined concomitantly with the rise in serum testosterone. At diagnosis, inhibin B was elevated and decreased throughout therapy, remaining in the upper normal prepubertal range. In patients with testicular volume above 4&#8201;mL AMH remained higher in spite of suppressed FSH. After treatment withdrawal, inhibin B rose towards normal pubertal levels. In conclusion, AMH did not decrease in patients <1 yr reflecting the lack of androgen receptor expression in Sertoli cells in early infancy. Serum inhibin B might result from the contribution of two sources: the mass of Sertoli cells and the stimulation exerted by FSH. Sertoli cell markers might provide additional tools for the diagnosis and treatment followup of boys with central precocious puberty.","palabras_clave":"PUBERTY|AMH|INHIBIN B|MALE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64487,"idioma_id":5,"titulo":"Hormona anti-Mülleriana (AMH) como herramienta diagnóstica en la mujer.","resumen":"La hormona antimülleriana (AMH) es producida por el  testículo fetal e infantil en altas cantidades y por el testículo adulto y el  ovario, en cantidades más moderadas. En el ovario, la expresión de la AMH se limita a las células de  la granulosa de los folículos primarios, secundarios y antrales; los folículos  preantrales y antrales pequeños son los principales productores. Los niveles de  AMH persisten relativamente estables durante la infancia y la pubertad, y disminuyen  progresivamente en la cuarta y quinta décadas de la vida hasta hacerse  indetectables aproximadamente 5 años antes del último ciclo menstrual. Así, la  determinación de AMH sérica es una herramienta útil para valorar la masa de  células foliculares: los niveles bajos o indetectables de AMH reflejan una  escasa o nula reserva folicular (por ejemplo: insuficiencia ovárica primaria  congénita o adquirida), en tanto que los niveles elevados indican un exceso de  folículos pequeños (por ejemplo: síndrome de ovario poliquístico y tumores de  la granulosa). La determinación de la   AMH se ha transformado en un marcador esencial en la  evaluación de pacientes que recurren a técnicas de reproducción asistida, ya  que permite estimar las probabilidades de éxito y también decidir el protocolo  de estimulación por utilizar de modo de evitar el riesgo de una  hiperestimulación ovárica.","palabras_clave":"Ovario poliquístico|Fertlización asistida|Reserva ovárica|Reproducción"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64488,"idioma_id":4,"titulo":"Light and soil moisture effects on biomass and its allocation in Osmorhiza depauperata Philippi (Apiaceae)","resumen":"Changes in forest openings affect light qualityand quantity, and the magnitude of rainfall that reachesthe soil surface. Osmorhiza depauperata, a geophyte,acclimates to changes imposed because of forest openings.We studied which changes in biomass allocationallow acclimation of O. depauperata to the variousenvironments that this species inhabits, and where itdevelops better. Three light intensities (I4 = 4 %,I26 = 26 %, I64 = 64 % of ambient sunlight) and twomoisture levels (M40 = 40\u201360 %, M80 = 80\u2013100 %field capacity) were evaluated on O. depauperata undergreenhouse conditions. Plant biomasses per pot were0.81, 0.56 and 0.48 g at I26, I4 and I64 light intensities,respectively, after one growing season. The biomassallocation to aboveground tissues and leaf area decreasedas light intensity increased. Soil moisture modifiedonly belowground biomass and weight of fine roots.The interaction between soil moisture content and lightintensity was consistent. This was because of a significantreduction in total plant biomass under high bothsoil moisture content and high light intensity. Osmorhizadepauperata growth was favored most at medium lightintensities. Changes in biomass allocation among variousorgans allow this species to inhabit forest habitatswith different light intensities.","palabras_clave":"Light availability|forest canopy gaps|Osmorhiza depauperata|water availability"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64489,"idioma_id":4,"titulo":"'Seasonal diet of Lama guanicoe (Camelidae: Artiodactyla) in a heterogeneous landscape of South Patagonia","resumen":"Large diversity of habitat types, plant species assemblages, and silvopastoral activities in Nothofagus spp. forests condition the foodavailability for native herbivores. This work evaluates the seasonal diet of Lama guanicoe linked to plant life forms and habitat typesin sub-Antarctic forests. The study evaluated vegetation availability and habitat types by floristic surveys (n = 206) in Tierra delFuego, and seasonal diet of L. guanicoe by a micro-histological analysis of feces (n = 4 in four areas, during four season). Open landsshowed the highest plant richness, while lowest values corresponded to primary Nothofagus pumilio forests. Nothofagus pumilio(21.6 %), Carex spp. (17.2 %), Misodendrum spp. (10.6 %) and Deschampsia spp. (8.6 %) were the most frequent items found infeces. Significant differences were seasonally found in diet composition and trophic niche breadth: grasses were all consumed alongthe year, while tree browsing decreased and the proportion of shrubs increased in winter. This study highlights the widespread useof different habitats by guanaco, including harvested forests. In summer and spring Nothofagus spp. forests were the main source offood for guanacos, depending more on open lands during winter. The knowledge of plant-native herbivores interactions in productivelandscapes could improve the management plans towards an ecologically sustainable strategy.","palabras_clave":"GRASSES|GUANACO|LANDSCAPE|PLANT ASSEMBLAGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64490,"idioma_id":4,"titulo":"Natural dynamics and thinning response of young lenga (Nothofagus pumilio) trees in secondary forests of Southern Patagonia","resumen":"Se exponen los resultados de raleos efectuados en el año 2006 en un bosque de lenga en fase de crecimiento optimo inicial.","palabras_clave":"raleo|copas|retención|mortalidad"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64491,"idioma_id":4,"titulo":"Are silvopastoral systems compatible with forest regeneration? An integrative approach in southern Patagonia","resumen":"We used an integrated approach to analyze the influence of silvopastoral management on the biotic and abiotic factors influencing on the natural recruitment of native forests in Tierra del Fuego (Argentina). The probabilities of transition from flowers to seeds to seedlings were estimated in forests with silvopastoral management (i.e. stands that had been both grazed and thinned), secondary growth stands, and unmanaged old-growth areas. Pre-dispersal losses were caused by microclimate and insect predation acting on flowers and immature fruits. Post-dispersal losses resulted from the impact of reduced litterfall and microclimate. But the most critical stage of the overall cycle was seedling establishment. Silvopastoral practices and stand age modified the main drivers of regeneration. While flowering was unaffected by management, fruiting and seed production were more successful in unmanaged forests. Seedling establishment and survival were favored by canopy cover reduction in silvopastoral stands. The increase of solar radiation and soil moisture in managed forests positively influenced the seedling establishment and survival, while in second-growth forests it was limited by suitable micro-site availability. Thinning practices aimed at increasing the understory heterogeneity in mature forests and restoring canopy complexity of second-growth stands could be suitable for sustainable management of these temperate forests.","palabras_clave":"ciclo de reproduccion|flores|semillas|regeneracion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64492,"idioma_id":4,"titulo":"Survival and growth of Nothofagus pumilio seedlings under several microenvironments after variable retention harvesting in southern Patagonian forests.","resumen":"Context Variable retention prescriptions for Nothofagus pumilio forests provide for biodiversity conservation and natural regeneration by controlled opening of the canopy. Harvesting generates different microenvironments which present dissimilar conditions for seedling establishment, due to positive or negative influences over biotic and abiotic factors.Aims This study evaluated seedling survival and performance in different microenvironments within the harvested stands. Tested hypotheses stated that seedling stress and performance were influenced by harvesting due to changes in Handling forest structure, microclimate, soil properties, and nutrient availability.Methods In the stands harvested by variable retention, five contrasting microenvironments were selected as treatments for the experiments and sampling. Environmental variables were related to ecophysiological, seedling survival, and performance.Results The modification of forest structure (crown cover and tree density) and the presence of coarse woody debris greatly affect the effective rainfall and global radiation reaching understorey level, influencing seedling stress and consequently survival and performance. Harvesting also modifies soil properties (e.g., soil bulk density) and coarse woody debris accumulation which in turn influences soil moisture and\/or solar radiation levels. Analyses showed that seedlings received benefits of microenvironment variation after harvesting. Areas covered with middle or fine woody debris presented regeneration with better ecophysiological response and seedling performance, although dispersed retention areas (far away from remnant trees) and roads could also present suitable conditions for seedling survival and performance.Conclusions The proportion of different microenvironments in the harvested forests will determine the amount of natural recruitment of regeneration and consequently the success of proposed silvicultural management. Forest practices must be manipulated to increase the proportion of favorable microenvironments (e.g., woody debris), allowing greater natural regeneration success during the first years afterharvesting.","palabras_clave":"Aggregated retention|Dispersed retention|Microenvironments|Light availability"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64493,"idioma_id":5,"titulo":"Buenos Aires, ¿una ciudad sin techo?","resumen":"Actualmente en la Ciudad de Buenos Aires 142.924 hogares desarrollan su vida cotidiana en condiciones habitacionales deficitarias. En esos hogares conviven 470.966 habitantes que representan al  16,7% de la población de la Ciudad. Una mirada  retrospectiva sobre el problema habitacional en Buenos Aires, pone en evidencia  que la situación se ha agravado durante las últimas décadas. En la Ciudad,  entre 1991 y 2001, de acuerdo al Instituto de Vivienda de la Ciudad, la  incidencia del déficit habitacional aumentó tanto en términos relativos como  absolutos. El déficit habitacional afectaba al final de ese periodo a unos  86.185 hogares (que representaban el 8,4% del total de los hogares residentes  en la CABA): 5.690 hogares más que en 1991. En 1991 el déficit habitacional  afectaba a 80.495 hogares que representaban el 7,9% del total de hogares  residentes en la Ciudad, según datos de la Dirección Nacional de Políticas  Habitacionales de la Subsecretaria de Vivienda y Desarrollo Urbano. En ese marco,  229.306 porteños estaban afectados por situaciones habitacionales deficitarias.  En 2010, a los 86.185 hogares, se suman 56.739 nuevos.","palabras_clave":"DEFICIT HABITACIONAL|HÁBITAT POPULAR|DERECHO A LA CIUDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64494,"idioma_id":4,"titulo":"Thyroid hormones-membrane interaction: reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential.","resumen":"Abstract      Differential scanning calorimetry (DSC), mixed monomolecular layers and fluorescence spectroscopy techniques were applied to investigate the effect of thyroid hormones (THs) on the biophysical properties of model membranes. We found that both 3,3¡ä,5-triiodo-l-thyronine (T3) and 3,5,3¡ä,5¡ä-tetraiodo-l-thyronine (T4) induce a broadening of the calorimetric main phase transition profile and reduce the transition enthalpy in liquid-crystalline state of dipalmitoylphosphatylcholine (DPPC) multilamellar vesicles. Tm changes from 41&nbsp;¡ãC to 40&nbsp;¡ãC compared to pure DPPC. When the experiments were done by adding THs to preformed multilamellar vesicles a second broader component in the DSC scan also appears at 20&nbsp;min of incubation and becomes gradually more prominent with time, indicating a progressive alteration of lipid phase induced by THs. Analysis of surface pressure-molecular area isotherms in mixed monolayers of THs with either DPPC or 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) at air¨Cwater interface indicated a reduction in molecular area for THs\/lipid mixtures at all surface pressures. A substantial decrease in surface potential in mixed lipid\/THs monolayers at all surface pressures were observed for both phospholipids without affecting the mixed monolayer integrity. The data of mixed lipid\/THs behavior support the establishment of lateral miscibility. Alterations of bidimensional liquid expanded&nbsp;¡ú&nbsp;liquid condensed phase transition observed for DPPC\/THs mixed monolayers are compatible with the changes observed in DSC. The transverse movement of THs and the decrease of dipole potential were also observed in single unilamellar vesicles by using appropriate fluorescent probes.","palabras_clave":"Thyroid hormones|Differential scanning calorimetric|Mixed monomolecular layer|Membrane potential"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64495,"idioma_id":4,"titulo":"Chymotrypsin - Eudragit Complex Formation","resumen":"Abstract            Eudragit® L100 (EuL) and Eudragit® S100 (EuS) are synthetic polyanions differing on their electric charge density. They interact with chymotrypsin (ChTRP), a basic protein forming soluble and non-soluble complexes. The complex formation was studied by dynamic light scattering, isothermal titration calorimetry, native fluorescence emission, circular dichroism and thermodynamical thermal stability of the enzyme. EuS was able to bind 33 ChTRP molecules while EuL, 60. The binding of ChTRP to both Eu was slightly endothermic and the entropic factor was responsible for the soluble complexes formation. The ChTRP-Eu size increases with pH and the binding of ChTRP to Eu modifies the Eu hydrodynamic radium. The interaction of ChTRP with Eu did not modify its secondary or tertiary structure. The thermal stability of ChTRP was increased when it interacted with both Eu.","palabras_clave":"eudragit|chymotrypsin|polyelectrolytes|calorimetry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64496,"idioma_id":4,"titulo":"Environment-driven reactivity of H2 on PdRu surface alloys","resumen":"The dissociative adsorption of molecular hydrogen on PdxRu1-x\/  Ru(0001) (0 < x < 1) has been investigated by means of He atom  scattering, Density Functional Theory and quasi-classical trajectory  calculations. Regardless of their surroundings, Pd atoms in the  alloy are always less reactive than Ru ones. However, the reactivity  of Ru atoms is enhanced by the presence of nearest neighbor Pd  atoms. This environment-dependent reactivity of the Ru atoms in  the alloy provides a sound explanation for the striking step-like  dependence of the initial reactive sticking probability as a function  of the Pd concentration observed in experiments. Moreover, we  show that these environment-dependent effects on the reactivity  of H2 on single atoms allow one to get around the usual constraint  imposed by the Brønsted?Evans?Polanyi relationship between the  reaction barrier and chemisorption energy.","palabras_clave":"SURFACES|ALLOYS|HYDROGEN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64497,"idioma_id":4,"titulo":"Quantum phase transition between one-channel and two-channel Kondo po- larons","resumen":"For a mobile spin-1\/2 impurity, coupled antiferromagnetically to a one-dimensional gas of fermions, perturbative ideas have been used to argue in favor of two-channel Kondo behavior of the impurity spin. Here we combine general considerations and extensive numerical simulations to show that the problem displays a novel quantum phase transition between two-channel and one-channel Kondo screening upon increasing the Kondo coupling. We construct a ground-state phase diagram and discuss the various nontrivial crossovers as well as possible experimental realizations.","palabras_clave":"quantum impurities"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64498,"idioma_id":4,"titulo":"Immune response to Streptococcus pneumoniae in asthma patients. Comparison between stable situation and exacerbation.","resumen":"In Argentina, more than 3 million people suffer from asthma, with numbers rising. When asthma patients acquire viral infections which, in turn, trigger the asthmatic response, they may develop subsequent bacterial infections, mainly by Streptococcus (S.) pneumoniae. This encapsulated Gram+ bacterium has been considered historically a T cell-independent antigen. Nevertheless, several papers describe the role of T cells in the immune response to S. pneumoniae.We evaluated the response to S. pneumoniae and compared it to the response to Mycobacterium (M.) tuberculosis, a different type of bacterium that requires a T helper type 1 (Th1) response, in cells from atopic asthmatic children, to compare parameters for the same individual under exacerbation and in a stable situation whenever possible.We studied asthma patients and a control group of age-matched children, evaluating cell populations, activation markers and cytokine production by flow cytometry, and cytokine concentration in serum and cell culture supernatants by enzymelinked immunosorbent assay (ELISA). No differences were observed in gd T cells for the same patient in either situation, and a tendency to lower percentages of CD4+CD25hi T cells was observed under stability. A significantly lower production of tumour necrosis factor (TNF)-a and a significantly higher production of interleukin (IL)-5 was observed in asthma patients compared to healthy individuals, but no differences could be observed for IL-4, IL-13 or IL-10. A greater early activation response against M. tuberculosis, compared to S. pneumoniae, was observed in the asthmatic patients? cells. This may contribute to explaining why these patients frequently acquire infections caused by the latter bacterium and not the former. ","palabras_clave":"activation|asthma|atopy|infections|T cells"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64499,"idioma_id":4,"titulo":"Study of biochemical biomarkers in freshwater prawn Macrobrachium borellii (Crustacea: Palaemonidae) exposed to organophosphate fenitrothion","resumen":"Several agrochemicals like organophosphates are extensively used to control pests in agricultural practices but they also adversely affect non-target fauna. The effect of organophosphorous fenitrothion on the prawn Macrobrachium borellii was evaluated. The 96-h LC50 was determined. Activity of superoxide dismutase, catalase, glutathione-S-transferase and lipid oxidation levels, were evaluated in the hepatopancreas from adults exposed to sublethal fenitrothion concentrations for 1, 2, 4 and 7 days. In addition, superoxide dismutase mRNA expression, acetylcholinesterase inhibition and haemocyte DNA damage were determined. The 96-h LC50 was 4.24 &#956;g\/l of fenitrothion. Prawn exposed to sublethal FS concentrations showed an increase of both catalase and superoxide dismutase activities, mainly after 2 and 4 days exposure and an increase of glutathione-S-transferase activity from day 2 to day 7 while lipid oxidation levels increased mainly on day 1. Superoxide dismutase transcripts were significantly higher in fenitrothion -treated prawns, indicating an induction mechanism. Hemolymph analysis showed that while acetylcholinesterase activity decreased after 2 days, haemocytes displayed most DNA damage after 7-day exposure to fenitrothion.   \tThese results indicate that prawn enzymes are highly sensitive to fenitrothion exposure, and these biological responses in M. borellii could be valuable biomarkers to monitor organophosphorous contamination in estuarine environments.","palabras_clave":"Biomarker|Fenitrothion|Organophosphorous|Oxidative stress|Prawn|Toxicity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64500,"idioma_id":4,"titulo":"Lipases as efficient catalysts in the synthesis of monomers and polymers with biomedical applications","resumen":"Enzymatic polymerization shows to be an advantageous approach in polymer chemistry, particularly in the case of obtaining polymers required for biomedical applications, which are intented for human consumption. Enzymes apply procedures that are environmentally friendly involving waste minimization, hazard reduction, the efficient use of energy and the use of renewable sources. This paper reviews selected examples of the recent progress in lipase-catalyzed polymerization. The application of this hydrolase in the synthesis of polyesters, polycarbonates and polyamides following traditional mechanisms is covered. Moreover, lipase-catalyzed synthesis of novel vinyl copolymers and monomers is described.","palabras_clave":"Lipase|Polymerization|Monomers|Biomedical applications"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64501,"idioma_id":4,"titulo":"New metabolites of drospirenone obtained in Mucorales fungi culture","resumen":"Microbial transformation of 6,7,15,16-dimethylene-3-oxo-17-pregn-4-ene-2,17-carbo-lactone,the well known contraceptive drospirenone (1), using fungal cells was carried out. Six fungal strains of different species of Order Mucorales were evaluated in this study, namely Absidia corymbifera BAFC 1072, Absidia corymbifera BAFC 1080, A. coerulea, Mucor plumbeus BAFC 2314, Rhizopus oryzae and Syncephalastrum racemosum. Four products were obtained by hydroxylation at C-11 and C-2 and epimerization at C-17 of drospirenone by A. corymbifera BAFC 1072, A. coerulea and S. racemosum. The structures were elucidated as 6,7,15,16-dimethylene-11-hydroxy-3-oxo-l7-pregn-4-en-21,17-carbolactone (2), 6,7,15,16-dimethylene-11-hydroxy-3-oxo-l7-pregn-4-en-21,17-carbolactone (3), 6,7,15,16-dimethylene-11-hydroxy-3-oxo-l7-pregn-4-en-21,17-carbolactone (4) and 6,7,15,16-dimethylene-2-hydroxy-3-oxo-l7-pregn-4-en-21,17-carbolactone (5), on the basis of extensive spectral data including 2D NMR spectroscopy and MS.  Products 3, 4 and 5 were found to be new compounds. Several biotransformation parameters such as the employment of growing or resting cells,inoculumsize, agitationspeed, drospirenone concentration, temperature,pH and presenc of co-solvent were seen to be important to the optimization of the process.","palabras_clave":"Esteroides|Oxigenasas|Mucorales|hongos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64502,"idioma_id":4,"titulo":"The effect of introduced species on raptors","resumen":"Biological invasions are considered one of the major threats to the Earth?s biota and their prevention and control are widely suggested. A key step within this is to gather knowledge and data on the impacts of introduced species on native species. In such analysis it is important to consider both the negative effects and the fact that many ?non-natives? have grown to become key components of existing ecosystems. The effects of non-natives are particularly worrisome for raptors because they hold high trophic positions and their ecological role can structure native communities. In this paper we look at the impacts of introduced species on raptors, as well as the interest in studying these effects, through a review of the literature. The numbers of studies on raptors and introduced species as individual topics are rapidly increasing, but despite this we found few articles relating the two topics and fewer still with a clear aim of studying this relationship. Although the number of articles found was low, articles showing negative effects outnumbered articles showing positive ones. Negative effects identified included native prey decrease and direct or indirect poisoning through poisons aiming to protect productive introduced species from predators or to control introduced pests. Positive effects identified included distribution area extension facilitated by introduced species or non-native species as a food source. Very importantly, native species can become dependent on introduced species, and any control merits careful evaluation. In conclusion, the implications of non-native species for this key group of top predators and scavengers have been poorly considered, but merits special attention and specific design in future studies.","palabras_clave":"BIOLOGICAL INVASIONS|RAPTORS|ALIEN SPECIES|INTERACTIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64503,"idioma_id":4,"titulo":"Population Sex Ratios: Another Consideration in the Reintroduction - Reinforcement Debate?","resumen":"Reintroduction or reinforcement (RorR) of wild populations is a common conservation strategy. Many conservation projects involve the release of individuals of poorly studied species. This may lead to inefficient results or negative impacts on the conservation efforts. Here, we provide new insights into the conservation implications and potential consequences of a skew in the sex ratio of released birds and of the number of birds supplemented for the demography of a long-lived dimorphic bird species, the Andean condor (Vultur gryphus). We demonstrate that a RorR conservation program may be less effective in conserving a species if the sex ratios of the releases and the recipient populations are not considered. We also show that releases can reduce population declines but only if carried out over long periods (i.e., several decades). This can mean high costs for release programs and the added challenge of maintaining programs over time. If RorR programs are to be implemented, bearing in mind the importance of properly assessing their effectiveness, we urge conservation researchers and managers to consider the implications of sex ratio biases for wild populations, and particularly for dimorphic species with sexually despotic behaviour.","palabras_clave":"REINTRODUCTION|REINFORCEMENT|SEX RATIO|RELEASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64504,"idioma_id":4,"titulo":"Partial molecular characterization of different proviral strains of bovine leukemia virus","resumen":"Bovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2*0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.","palabras_clave":"BOVINE LEUKEMIA VIRUS|STRAINS|ENV SEQUENCE|LOW PROVIRAL LOAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64505,"idioma_id":4,"titulo":"Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR","resumen":"Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene and Bovine Leukemia Virus (BLV) infection profile. The presence of allele *1501 has been associated with high proviral load in peripheral blood while allele *0902 has been associated with low proviral load. The purpose of this study was to develop allele-specific real-time PCRs to identify cattle carrying alleles associated with resistance (BoLA DRB3*0902) or susceptibility (BoLA DRB3*1501) to the BLV progression. Specific primers were designed and differential amplification was carried out by real-time PCR and monitored by SYBR® Green dye in DNA samples from peripheral blood. Conditions were also adjusted for traditional PCR amplification (end point amplification). These methods are rapid, simple and suitable for high throughput screening, and could aid in marker-assisted selection of BLV-resistant and susceptible cattle.","palabras_clave":"BoLA DRB3|Bovine Leukemia Virus|Resistance|Susceptibility|PCR|Real-Time PCR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64506,"idioma_id":4,"titulo":"Statistical modelling of higher-order correlations in pools of neural activity","resumen":"Simultaneous recordings from multiple neural units allow us to investigate the activity of  very large neural ensembles. To understand how large ensembles of neurons process sensory information, it is necessary to develop suitable statistical models to describe the response variability of the recorded spike trains. Using the information geometry framework, it is possible to estimate higher-order correlations by assigning one interaction parameter  to each degree of correlation, leading to a (2^N &#8722; 1)-dimensional model for a population with N neurons. However, this model suffers greatly from a combinatorial explosion, and the number of parameters to be estimated from the available sample size constitutes the main intractability reason of this approach. To quantify the extent of higher than pairwise  spike correlations in pools of multiunit activity, we use an information-geometric approach within the framework of the extended central limit theorem considering all possible contributions from higher-order spike correlations. The identification of a deformation parameter  allows us to provide a statistical characterisation of the amount of higher-order correlations in the case of a very large neural ensemble, significantly reducing the number of parameters, avoiding the sampling problem, and inferring the underlying dynamical  properties of the network within pools of multiunit neural activity.","palabras_clave":"Extended central limit theorem|Large neural ensemble|Multiunit neural activity|Information geometry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64507,"idioma_id":4,"titulo":"Diel patterns in space use, food and metabolic activity of Galaxias maculatus (Pisces: Galaxiidae) in the littoral zone of a shallow Patagonian lake","resumen":"We detected that Galaxias maculatus exhibits a pattern where metabolic activity increases after sunrise and peaks between noon and sunset, but this species feeds in the afternoon, until several hours after sunset. Moreover, we showed that G. maculatus is observed in the littoral zone during the day, disappears completely from this zone after sunset and returns at sunrise. Littoral prey species are common in the diet of G.  maculatus, but this study showed that pelagic prey are also present during twilight and night hours in smaller individuals (<50 mm), which is related to habitat use. These behavioural rhythms are especially important for G. maculatus, which runs a high predation risk when consuming prey that is widely available outside the littoral zone.  This risk is ameliorated under the protection of low light intensity. Thus, G. maculatus is a key species linking lower trophic levels, such as the plankton community, to higher levels of native and exotic piscivores. These displacements of G. maculatus generate an active flow of energy and matter between habitats, with a potentially profound effect on the entire food network and energy dynamics of the lake.","palabras_clave":"METABOLIC RATE|FEEDING|HABITAT USE|GALAXIAS MACULATUS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64508,"idioma_id":5,"titulo":"Intercambio de mecanismos de resistencia entre bacterias gram negativas.","resumen":"La magnitud global de la resistencia a los antimicrobianos de uso clínico es alarmante adquiriendo una dimensión destacada en países de desarrollo intermedio quienes suelen ser cuantitativamente los más afectados por la emergencia de mecanismos de resistencia dado el acceso a tratamientos con antibióticos de reserva pero una pobre vigilancia o empleo no riguroso de medidas de contención de la resistencia.   En este trabajo se realizará una revisión sobre las estructuras genéticas movilizables, que si bien no son esenciales para las bacterias, aportan genes adicionales que le permiten una mejor adaptación a ambientes hostiles.   El intercambio de genes de resistencia entre las bacterias permite equipar a un microorganismo sensible a antibióticos con un verdadero arsenal de mecanismos de resistencia, incluso en un único evento de intercambio. La transferencia horizontal de genes de resistencia entre bacilos gram negativos es debida en gran parte a plásmidos (más o menos promiscuos) y a los elementos transponibles e integrones que pueden formar parte del o de los replicones presentes en estos microorganismos.   Los integrones son plataformas genéticas que han despertado gran interés desde el punto de vista clínico ya que algunos de ellos vehiculizan genes de resistencia a los antimicrobianos. Están formados por un fragmento que codifica una integrasa (intI) seguido por una secuencia attI, sistema que permite la captura de los genes en casetes (que codifican para diferentes mecanismos de resistencia). Se habla, en general, de integrones ?móviles? a aquellos asociados a secuencias de inserción, transposones y\/o plásmidos conjugativos, que en su mayoría median mecanismos de resistencia, y ?super? integrones, de localización cromosómica con grandes arreglos de genes en casetes.","palabras_clave":"integrones|transposones|plásmidos|gen en cassette"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64509,"idioma_id":4,"titulo":"Effect of radiation intensity on the outcome of postharvest UV-C treatments","resumen":"tStudies on the use of UV-C radiation of fresh produce have focused on the selection of appropriate doses(energy per unit area) for different commodities, but little attention has been placed on the effect ofradiation intensity (dose per unit time). In this study, tomatoes (Solanum lycopersicum cv. Elpida) andstrawberries (Fragaria × ananassa cv. Camarosa), were harvested (breaker and 100% of surface red colorrespectively) and treated with 4 kJ m&#8722;2of UV-C, at low (3 W m&#8722;2) or high (33 W m&#8722;2) radiation intensities.Untreated fruits were used as controls. After the treatments and at different storage times the incidence ofpostharvest rots and the changes in fruit physical and chemical properties were determined. UV-C treat-ments reduced decay, with the effects being were more marked in fruit exposed to high intensities. Moldcounts were unaffected by the treatments, suggesting that improved disease control did not result fromgreater germicide effect. In both fruit species exposure to UV-C radiation delayed ripening, evidencedas lower color development, pigment accumulation and softening. UV-C-treated fruit maintained betterquality than the control. In strawberry, high intensity treatments were more effective to prevent dete-rioration than in tomato where the differences between UV-C treatments were subtler. Soluble solids,titratable acidity and ethanol soluble antioxidants were not affected regardless of the UV-C intensity.Consumer tests showed higher preference of fruit treated at high UV-C intensity. Results show that inaddition to the applied dose, radiation intensity is a main factor determining the effectiveness of UV-Ctreatments and should not be over-sighted. For a given dose, increasing radiation intensity may in somecases maximize the benefits of UV-C on fruit quality, while significantly reducing the treatments time.© 2013 Elsevier B.V. All rights reserved.","palabras_clave":"fluency|irradiation|quality|tomato|strawberry"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64510,"idioma_id":4,"titulo":"Stable isoperimetric surfaces in super-extreme Reissner-Nordström","resumen":"We study isoperimetric surfaces in the Reissner?Nordström spacetime,   with emphasis on the cuasilocal inequality between area and charge. We   analyze the stability of the isoperimetric spheres and we found that   there is a lower bound on the area in terms of the charge, and that the   inequality is saturated in the transition from the superextremal to the   subextremal case. We also derive a general inequality between area and   charge for stable isoperimetric surfaces in maximal electro-vacuum   initial data.","palabras_clave":"ISOPERIMETRIC SURFACES|REISSNER-NORDSTROM|GEOMETRIC INEQUALITIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64511,"idioma_id":4,"titulo":"Fluvial architecture variations linked to changes in accommodation space: Río Chico Formation (Late Paleocene), Golfo San Jorge basin, Argentina","resumen":"The Upper Paleocene Río Chico Formation is a 50\u2013180 m thick &#64258;uvial succession developed in a passive-margin  setting, Golfo San Jorge basin, Central Patagonia, Argentina. A detailed description and interpretation of outcrops  was carried out, analyzing exposures from the northern basin margin to the most complete successions at the  southern depocenter. The unit is characterized by a regional &#64258;uvial system that &#64258;owed to the south-east. Five  main lithofacies associationswere de&#64257;ned: (I) active &#64258;uvial channels,with three sub-types: braided,meandering  and low-sinuosity, (II) sheet-&#64258;ood deposits, (III) proximal &#64258;oodplain (natural levee and crevasse-splay),  (IV) distal &#64258;oodplain, and (V) abandoned channels.  Lateral\/vertical changes in &#64258;uvial architecture of the Río Chico Formation were recognized by variations  in preserved thickness, &#64258;uvial styles, geometry of &#64258;uvial channels, regional paleo&#64258;ow directions, and  channel\/&#64258;oodplain ratios. Close to the northern basin margin, the &#64258;uvial succession is 50\u201360 m thick,  composed of braided channels, sheet-&#64258;ow deposits, and high channel\/&#64258;oodplain ratio. In a basinward direction,  the alluvial succession increases to 180 m in thickness, the dominant &#64258;uvial styles change to low-sinuosity and  meandering channels and channel\/&#64258;oodplain ratio reduces.  The &#64258;uvial architecture of the Río Chico Formation shows two main depositional trends that resulted from  changes in accommodation space across the basin. The interpreted break-point coincides with the underlying  Cretaceous basin-boundary, thus the synsedimentary extensional reactivation of the pre-existing tectonic  lineament generated differential subsidence, delimiting two different accommodation settings.","palabras_clave":"FLUVIAL ARCHITECTURE|ACCOMMODATION SPACE|RIO CHICO FORMATION|GOLFO SAN JORGE BASIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64512,"idioma_id":5,"titulo":"Implementación y evaluación de múltiples estrategias de enseñanza en cursos de probabilidad y estadística para Ingeniería","resumen":"Las dificultades en la asignatura Probabilidad y  Estadística que presentaron los alumnos del ciclo básico  de carreras de Ingeniería de la Universidad Nacional de  La Matanza, motivaron a los docentes a la  implementación de múltiples estrategias de enseñanza,  con el fin de mejorar el rendimiento académico de los  alumnos. A partir de 2009 se implementaron cambios  que fueron evaluados a través de la perspectiva de  docentes y alumnos. Los cambios incluyen múltiples  estrategias didácticas, tales como clases teórico prácticas  con soporte tecnológico y papel,  autoevaluaciones, talleres de resolución de problemas,  utilización de foros virtuales, análisis de datos con  software Excel y utilización de simulaciones. Los  resultados muestran que ha mejorado el aprendizaje de  los alumnos y han aumentado los porcentajes de  aprobación. Además se evidencian en los análisis que  los alumnos no utilizan todos los recursos disponibles,  mostrando preferencia por los talleres de resolución de  problemas y la utilización de foros virtuales para la  discusión de la guía de problemas. Las encuestas a  alumnos muestran un cambio progresivo y positivo de la  percepción sobre la dificultad de la asignatura y la  posibilidad de aprobarla","palabras_clave":"PROBABILIDAD Y ESTADISTICA|FOROS VIRTUALES|INGENIERIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64513,"idioma_id":5,"titulo":"Discapacidad e inclusión en el ámbito de la educación superior universitaria a partir del modelo social de la discapacidad","resumen":"El artículo aborda desde el reconocimiento de las representaciones sociales, la   identificación, en los discursos de los docentes, de dos modelos sobre la   discapacidad, el Modelo Médico y el Modelo Social. En la actualidad, si bien el   Modelo Social, se constituye como el paradigma actual, superador al modelo   Médico, presentando como una de los rasgos centrales, el reconocimiento de la   inclusión y la necesidad del planteo de interrelación de factores del entorno   social, conviven en las formas de comprender la discapacidad, el Modelo   Médico, que ubica principalmente a la discapacidad, como un problema   individual, de la persona que presenta una discapacidad. En este marco se   presenta el análisis de entrevistas realizadas a sujetos, que como docentes en el ámbito universitario. En el discurso de los docentes se   analizaron las Representaciones Sociales, en cuanto a proximidades al Modelo   Social o al Modelo Médico. Como metodología se utilizaron dos estrategias   diferenciadas, el análisis de contenido y análisis lexicométrico. Los resultados   fueron convergentes y los discursos muestran rasgos de ambos modelos.","palabras_clave":"enseñanza superior|discapacidad|representación social"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64514,"idioma_id":5,"titulo":"Sobre la resurrección de los sentidos: alteridad, ética y estética en la propuesta bajtiniana (reseña)","resumen":"Sobre la resurrección de los sentidos: alteridad, ética y estética en la propuesta bajtiniana.  Reseña sobre el texto:  Mijaíl Bajtín; Yo también soy. (Fragmentos sobre el otro). Selección, traducción, comentarios de Tatiana Bubnova. México: Taurus. 2000. 172 pág.  En el dossier 'Cuidar y ser cuidado en nuestra cultura. Otra ética, otra estética para la vida', Silvia Gattino (comp.)","palabras_clave":"ETICA|LENGUAJE|SENTIDOS SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64515,"idioma_id":4,"titulo":"PERSONALIZED WEB ACCESSIBILITY USING CLIENT-SIDE REFACTORING","resumen":"According to W3C accessibility standards, most Web applications are neither accessible nor usable for people with disabilities. Developers often solve this problem by building separate accessible applications, but these are seldom usable and typically offer less functionality than the original. Another common solution is to maintain a single application, but create an accessible view by applying on-the-fly transformations to each requested page ? a solution that rarely suits all audiences. A third solution is described here: let users improve Web accessibility in their client browsers through interface refactorings, which offer many customized, accessible views of a single application.","palabras_clave":"Web|Accesibilidad|Usabilidad|Refactoring"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64516,"idioma_id":4,"titulo":"Detecting Web requirements conflicts and inconsistencies under a model-based perspective","resumen":"Web requirements engineering is an essential phase in the software   project life cycle for the project results. This phase covers different   activities and tasks that in many situations, depending on the analyst´s   experience or intuition, help getting accurate specifications. One of   these tasks is the conciliation of requirements in projects with   different groups of users. This article presents an approach for the   systematic conciliation of requirements in big projects dealing with a   model-based approach. The article presents a possible implementation of   the approach in the context of the NDT (Navigational Development   Techniques) Methodology and shows the empirical evaluation in a real   project by analysing the improvements obtained with our approach. The   paper presents interesting results that demonstrate that we can get a   reduction in the time required to find conflicts between requirements,   which implies a reduction in the global development cost","palabras_clave":"Requerimientos|Inconsistencias|WebSpec|NDT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64517,"idioma_id":4,"titulo":"Geoarchaeological review of the agricultural Pre-Hispanic period knowledge at the lowlands of Northwest Argentina","resumen":"The objective of this paper is to present in a synthetic and organized way the advances in the geoarchaelogical field at the Selvas Occidentales region (Northwest Argentina lowlands), during agricultural Pre-Hispanic Period. We understand by geoarchaeology the use of methods and techniques of geosciences to solve archaeological problems, where the main objective is to reconstruct man behavior and culture. Bibliographic review was difficult because the material is scarce and disorganized with a lack of specific antecedents on the subject. Nevertheless the information is enough to initiate a new approach to the archaeology of the region within a comprehensive framework which could result into the integration of important variables for the region and culturally related sectors.","palabras_clave":"Geoarqueologia|Selvas Occidentales|Tierras Bajas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64518,"idioma_id":4,"titulo":"Reading and note taking in monological and dialogical classes in the Social Sciences","resumen":"This qualitative study explores the uses of reading and note-taking in two pre-service teacher training Social Sciences courses. Data analysis of in-depth interviews with professors and students, class observations and course materials suggested two polar teaching styles according to how bibliography was included in the course and the presence or absence of dialogicality. In one course, the professor assumed that students should read texts on their own prerogative. As monological lectures were given, they mostly studied from their class-notes. In the other course, the professor held class discussions based on readings that took place in and outside the classroom. According to students, this prompted them to use their class-notes to re-signify and consider the relevance of the information read, with talking, reading, and note-taking contextualizing each other. The dialogical teaching style merged literacy practices and interwove them with disciplinary contents, promoting students´ active approach to meaning construction when learning.&nbsp;","palabras_clave":"lectura|enseñanza|historia|formación docente"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64519,"idioma_id":5,"titulo":"Intervenciones docentes para leer en ciencias sociales: exploración en un quinto año de secundaria con alumnos de sectores socioeconómicos desfavorecidos","resumen":"El presente trabajo se enmarca dentro de una tesis doctoral en curso, acerca de leer y escribir para aprender Ciencias Sociales en una escuela secundaria a la que asisten alumnos de sectores socioeconómicos desfavorecidos. Según las corrientes 'escribir a través del currículo' y 'alfabetizaciones académicas', entendemos que la lectura y la escritura han de ser guiadas y retroalimentadas por los profesores de las distintas asignaturas, dado que estas herramientas -esenciales para aprender los contenidos disciplinares- solo se adquieren situadamente. En este artículo, nos centramos en las intervenciones docentes que facilitan el leer para aprender. Para ello, observamos una secuencia de cuatro clases en quinto año y entrevistamos al docente de la materia Estudios Sociales Argentinos. El análisis de este material muestra que se proponen lecturas colectivas y se destina tiempo didáctico para enseñar diversas formas de abordar un texto para comprenderlo. Especialmente, lo que parece sostener estas propuestas es el acompañamiento que realiza el profesor al intervenir durante los momentos de lectura en clase para fortalecer la autoestima de los estudiantes y explicitar confianza en su capacidad de aprendizaje. DISPONIBLE EN: http:\/\/erevistas.saber.ula.ve\/index.php\/legenda\/article\/view\/4578","palabras_clave":"escuela secundaria|enseñanza|lectura|escritura"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64520,"idioma_id":4,"titulo":"An Aspect-Oriented Approach for  Spatial Concerns in Web Applications","resumen":"The growing availability of on-line geographical   information, since the advent of open map servers in the 2000s,   originated a new generation of Web applications, those which combine   'conventional' Web functionality with typical features of   traditional Geographic Application System (GIS). The rapid growth in   number and complexity of Web applications with geo-referenced data   together with the need to support fast requirements change, demands   for increased modularity. The volatility of some of these changing   requirements, both in the scope of their geographic nature or in the   period of time in which they are valid, stresses the importance of   the applications' modularity. A solution is to take into   consideration the crosscutting nature of these requirements and   decouple their realization from 'conventional' requirements in   separate software modules. This paper proposes an   end-to-end Aspect-Oriented approach to deal with spatial   requirements from the early stages of applications development   throughout to implementation. A significant contribution of this   approach is the characterization of the most common spatial   requirements in Web-GIS applications","palabras_clave":"GIS|Aspectos|Concerns|Spatial"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64521,"idioma_id":4,"titulo":"Epigenetics of Insulin Resistance: An Emerging Field in Translational Medicine","resumen":"Abstract In this article, we review the current knowledgeof and recent insights into the role of epigenetic factors inthe development of insulin resistance (IR), with emphasis onperoxisome proliferator-activated receptor gamma coactivator1&#945; (PPARGC1A or PGC1&#945;) methylation on fetal programmingand liver modulation of glucose-related phenotypes.Wediscuss the pathogenesis of IR beyond the integrity of &#946;-cellfunction and illustrate the novel concept of mitochondrialepigenetics to explain the pathobiology of metabolicsyndrome-related phenotypes. Moreover, we discuss whetherepigenetic marks in genes of the circadian rhythm system areable to modulate insulin\/glucose-related metabolic functionsand place hypoxia inducible factor 1 &#945; (HIF1&#945;) as a part ofthe master CLOCK gene\/protein interaction network thatmight modulate IR. Finally, we highlight relevant informationabout epigenetic marks and IR so that clinicians practicing inthe community may envision future areas of medical interventionand predict putative biomarkers for early diseasedetection.","palabras_clave":"EPIGENETICS|INSULIN RESISTANCE|DNA METHYLATION|HISTONE MODIFICATIONS|PGC1A|CLOCK"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64522,"idioma_id":4,"titulo":"Systems Biology Elucidates Common Pathogenic Mechanisms between Nonalcoholic and Alcoholic-Fatty Liver Disease","resumen":"The abnormal accumulation of fat in the liver is often related either to metabolic risk factors associated with metabolic  syndrome in the absence of alcohol consumption (nonalcoholic fatty liver disease, NAFLD) or to chronic alcohol  consumption (alcoholic fatty liver disease, AFLD). Clinical and histological studies suggest that NAFLD and AFLD share  pathogenic mechanisms. Nevertheless, current data are still inconclusive as to whether the underlying biological process  and disease pathways of NAFLD and AFLD are alike. Our primary aim was to integrate omics and physiological data to  answer the question of whether NAFLD and AFLD share molecular processes that lead to disease development. We also  explored the extent to which insulin resistance (IR) is a distinctive feature of NAFLD. To answer these questions, we used  systems biology approaches, such as gene enrichment analysis, protein\u2013protein interaction networks, and gene  prioritization, based on multi-level data extracted by computational data mining. We observed that the leading disease  pathways associated with NAFLD did not significantly differ from those of AFLD. However, systems biology revealed the  importance of each molecular process behind each of the two diseases, and dissected distinctive molecular NAFLD and  AFLD-signatures. Comparative co-analysis of NAFLD and AFLD clarified the participation of NAFLD, but not AFLD, in  cardiovascular disease, and showed that insulin signaling is impaired in fatty liver regardless of the noxa, but the putative  regulatory mechanisms associated with NAFLD seem to encompass a complex network of genes and proteins, plausible of  epigenetic modifications. Gene prioritization showed a cancer-related functional map that suggests that the fatty  transformation of the liver tissue is regardless of the cause, an emerging mechanism of ubiquitous oncogenic activation. In  conclusion, similar underlying disease mechanisms lead to NAFLD and AFLD, but specific ones depict a particular disease  signature that has a different impact on the systemic context.","palabras_clave":"SYSTEMS BIOLOGY|AFLD|NFLD|GENES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64523,"idioma_id":4,"titulo":"Fetal metabolic programming and epigenetic modifications: a systems biology approach","resumen":"  A growing body of evidence supports the notion that epigenetic  changes such as DNA methylation and histone modifications,  both involving chromatin remodeling, contribute to fetal  metabolic programming. We use a combination of gene\u2013protein  enrichment analysis resources along with functional annotations  and protein interaction networks for an integrative approach to  understanding the mechanisms underlying fetal metabolic programming.  Systems biology approaches suggested that fetal  adaptation to an impaired nutritional environment presumes  profound changes in gene expression that involve regulation of  tissue-specific patterns of methylated cytosine residues, modulation  of the histone acetylation\u2013deacetylation switch, cell differentiation,  and stem cell pluripotency. The hypothalamus and  the liver seem to be differently involved. In addition, new putative  explanations have emerged about the question of whether  in utero overnutrition modulates fetal metabolic programming in  the same fashion as that of a maternal environment of undernutrition,  suggesting that the mechanisms behind these two fetal  nutritional imbalances are different. In conclusion, intrauterine  growth restriction is most likely to be associated with the induction  of persistent changes in tissue structure and functionality.  Conversely, a maternal obesogenic environment is most probably  associated with metabolic reprogramming of glucose and  lipid metabolism, as well as future risk of metabolic syndrome  (MS), fatty liver, and insulin (INS) resistance.overnutrition modulates fetal metabolic programming in  the same fashion as that of a maternal environment of undernutrition,  suggesting that the mechanisms behind these two fetal  nutritional imbalances are different. In conclusion, intrauterine  growth restriction is most likely to be associated with the induction  of persistent changes in tissue structure and functionality.  Conversely, a maternal obesogenic environment is most probably  associated with metabolic reprogramming of glucose and  lipid metabolism, as well as future risk of metabolic syndrome  (MS), fatty liver, and insulin (INS) resistance.","palabras_clave":"metabolic syndrome|fetal programming|epigenetics|DNA methylation|histone modifications"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64524,"idioma_id":4,"titulo":"Obstructive Sleep Apnea Is Associated with Fatty Liver and Abnormal Liver Enzymes: a Meta-analysis.","resumen":"AbstractBackground Obstructive sleep apnea (OSA) is associatedwith the cluster of clinical conditions that comprise themetabolic syndrome, including nonalcoholic fatty liver disease(NAFLD). Our primary purpose was to estimate theeffect of OSA on serum alanine aminotransferase (ALT) andaspartate aminotransferase (AST). Our secondary purposewas to investigate the potential influence of OSA on histologicalseverity of NAFLD to explore whether chronic intermittenthypoxia is associated with inflammation and fibrosis.Methods Our literature search identified 11 studies, fromwhich we extracted information about numbers of controlsubjects and OSA patients, and ALT, AST, and NAFLD.Results From a total of 668 OSA patients and 404 controls, wefound that the standardized difference in mean values of ALTand AST levels in patients with OSA was significantly differentfrom that in the controls. Meta-regression showed that theassociation was independent of body mass index and type 2diabetes. Fatty liver was associated with OSA in five studieswith 400 subjects. OSAwas significantly associated with liverfibrosis in 208 subjects, but not with lobular inflammation.Conclusions Routine assessment of liver enzymes and liverdamage should be implemented in OSA patients because theyhave an increase of 13.3 % of ALT and 4.4 % of AST levels,and a 2.6-fold higher risk of liver fibrosis when they haveNAFLD, which is 2.6 times more frequent in OSA patients.","palabras_clave":"OBSTRUCTIVE SLEEP APNEA|NAFLD|META-ANALYSIS|HYPOXIA|METABOLIC SYNDROME|ALT|AST"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64525,"idioma_id":4,"titulo":"Fatty Liver Is Associated with Transcriptional Downregulation of Stearoyl-CoA Desaturase and Impaired Protein Dimerization","resumen":"Aims and Methods: We evaluated the modulation of liver stearoyl-CoA desaturase-1 (Scd1) by dietary factors and insulin resistance (IR) in two experimental models of high-fat diet (HFD)-induced nonalcoholic fatty liver disease(NAFLD). The first model included Sprague Dawley (SD) rats that developed NAFLD without IR, and the second oneincluded a rat model of genetic IR and cardiovascular disease, the spontaneously hypertensive rats (SHR) and itsnormotensive, insulin-sensitive control Wistar-Kyoto (WKY). The adult rats were given standard chow diet (CD) orHFD for 10 weeks. In all the animals, we explored the hepatic Scd1 transcriptional activity and protein levels.insulin resistance (IR) in two experimental models of high-fat diet (HFD)-induced nonalcoholic fatty liver disease(NAFLD). The first model included Sprague Dawley (SD) rats that developed NAFLD without IR, and the second oneincluded a rat model of genetic IR and cardiovascular disease, the spontaneously hypertensive rats (SHR) and itsnormotensive, insulin-sensitive control Wistar-Kyoto (WKY). The adult rats were given standard chow diet (CD) orHFD for 10 weeks. In all the animals, we explored the hepatic Scd1 transcriptional activity and protein levels.We evaluated the modulation of liver stearoyl-CoA desaturase-1 (Scd1) by dietary factors and insulin resistance (IR) in two experimental models of high-fat diet (HFD)-induced nonalcoholic fatty liver disease(NAFLD). The first model included Sprague Dawley (SD) rats that developed NAFLD without IR, and the second oneincluded a rat model of genetic IR and cardiovascular disease, the spontaneously hypertensive rats (SHR) and itsnormotensive, insulin-sensitive control Wistar-Kyoto (WKY). The adult rats were given standard chow diet (CD) orHFD for 10 weeks. In all the animals, we explored the hepatic Scd1 transcriptional activity and protein levels.Results: HFD-fed rats of both strains developed severe NAFLD. Liver abundance of Scd1 mRNA was significantly decreased in HFD-fed rats regardless of the strain; SD-CD: 235±195 vs. SD-HFD 4.5±2.9, p&lt;0.0004, and SHR-CD:75.6±10.8 vs. SHR-HFD: 4.48±17.4, and WKY-CD: 168.7±17.4 vs. WKY-HFD: 12.9±17.4, p&lt;0.000001 (mean±SE,ANCOVA adjusted by HOMA). Analysis of liver Scd1 protein expression showed a particular pattern in the HFDgroups, characterized by the presence of high levels of a monomeric protein band (32.2?6.6 Kda, p&lt;0.003) anddecreased levels of a dimeric protein band (61.9?6.1 Kda, p&lt;0.02) regardless of the rat strain. Pharmacologicintervention with the peroxisome proliferator-activated receptor á agonist clofibrate reverted the liver phenotype andsignificantly modified the hepatic Scd1 transcriptional activity and protein expression.decreased in HFD-fed rats regardless of the strain; SD-CD: 235±195 vs. SD-HFD 4.5±2.9, p&lt;0.0004, and SHR-CD:75.6±10.8 vs. SHR-HFD: 4.48±17.4, and WKY-CD: 168.7±17.4 vs. WKY-HFD: 12.9±17.4, p&lt;0.000001 (mean±SE,ANCOVA adjusted by HOMA). Analysis of liver Scd1 protein expression showed a particular pattern in the HFDgroups, characterized by the presence of high levels of a monomeric protein band (32.2?6.6 Kda, p&lt;0.003) anddecreased levels of a dimeric protein band (61.9?6.1 Kda, p&lt;0.02) regardless of the rat strain. Pharmacologicintervention with the peroxisome proliferator-activated receptor á agonist clofibrate reverted the liver phenotype andsignificantly modified the hepatic Scd1 transcriptional activity and protein expression.HFD-fed rats of both strains developed severe NAFLD. Liver abundance of Scd1 mRNA was significantly decreased in HFD-fed rats regardless of the strain; SD-CD: 235±195 vs. SD-HFD 4.5±2.9, p&lt;0.0004, and SHR-CD:75.6±10.8 vs. SHR-HFD: 4.48±17.4, and WKY-CD: 168.7±17.4 vs. WKY-HFD: 12.9±17.4, p&lt;0.000001 (mean±SE,ANCOVA adjusted by HOMA). Analysis of liver Scd1 protein expression showed a particular pattern in the HFDgroups, characterized by the presence of high levels of a monomeric protein band (32.2?6.6 Kda, p&lt;0.003) anddecreased levels of a dimeric protein band (61.9?6.1 Kda, p&lt;0.02) regardless of the rat strain. Pharmacologicintervention with the peroxisome proliferator-activated receptor á agonist clofibrate reverted the liver phenotype andsignificantly modified the hepatic Scd1 transcriptional activity and protein expression.Conclusion: Diet-induced fatty liver is associated with the downregulation of hepatic Scd1 transcript and dedimerization of the protein, and these changes were not much affected by the status of peripheral IR.of the protein, and these changes were not much affected by the status of peripheral IR.Diet-induced fatty liver is associated with the downregulation of hepatic Scd1 transcript and dedimerization of the protein, and these changes were not much affected by the status of peripheral IR.","palabras_clave":"SCD1|NAFLD|SHR|PROTEIN DIMERIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64526,"idioma_id":4,"titulo":"Glycosylation of glycolipids in cancer: basis for development of novel therapeutic approaches","resumen":"Altered networks of gene regulation underlie many pathologies, including cancer. There are several proteins in cancer cells that are turned either on or off, which dramatically alters the metabolism and the overall activity of the cell, with the complex machinery of enzymes involved in the metabolism of glycolipids not being an exception. The aberrant glycosylation of glycolipids on the surface of the majority of cancer cells, associated with increasing evidence about the functional role of these molecules in a number of cellular physiological pathways, has received considerable attention as a convenient immunotherapeutic  target for cancer treatment. This has resulted in the development of a substantial number of passive and active immunotherapies, which have shown promising results in clinical trials. More recently, antibodies to glycolipids have also emerged as an attractive tool for the targeted delivery of cytotoxic agents, thereby providing a rationale for future  therapeutic interventions in cancer. This review first summarizes the cellular and molecular bases involved in the metabolic pathway and expression of glycolipids, both in normal and tumor cells, paying particular attention to sialosylated glycolipids (gangliosides). The  current strategies in the battle against cancer in which glycolipids are key players are then described.","palabras_clave":"gangliosides|glycolipids|glycosylation|cancer|immunotherapy|antibodies|immunotoxin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64527,"idioma_id":4,"titulo":"Structural mosaicity and electrical properties of pyrolytic SnO2:F thin films","resumen":"In this work we present structural and electrical properties of SnO2:F thin films deposited on glass substrate by the spray pyrolysis technique. The precursors of SnO2:F were synthesized by a sol?gel method, starting from a SnCl4·5H2O\/ethanol mixture and using NH4F\/H2O as a dopant. Different properties were observed depending on the deposition time of the films. The structural characteristics of the layers, like crystal size, preferential growth orientation and mosaicity, were studied by X-ray diffraction. These results were compared and correlated with those obtained from electrical measurements, such as carrier density, Hall mobility and resistivity. We have found that for longer deposition times, the mosaicity of the crystallites increases. This process is accompanied by a decrease in the resistivity.","palabras_clave":"Mosaicidad|Silicio|Película delgada"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64528,"idioma_id":4,"titulo":"Real-time study of protein adsorption kinetics in porous silicon","resumen":"This paper presents an optical method for real-time monitoring of protein adsorption using porous siliconself-supported microcavities as a label-free detection platform. The study combines an experimentalapproach with a physical model for the adsorption process. The proposed model agrees well with experimentalobservations, and provides information about the kinetics of diffusion and adsorption of proteinswithin the pores, which will be useful for future experimental designs.","palabras_clave":"Silicio Macroporoso|Cinética|Absorción de Proteinas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64529,"idioma_id":4,"titulo":"Influence of the crystalline orientations on microcrack initiation in low-cycle fatigue","resumen":"The present study aims at analyzing the crack initiation in an austenitic stainless steel in low-cycle fatigue. A fatigue test was carried out using a polished specimen. The surface of the specimen was observed in situ during the fatigue test, in order to establish the time of slip activity or crack initiation. After a number of cycles sufficient to initiate small cracks, the test was stopped and the surface observed by Scanning Electron Microscopy. The electron backscattered diffraction technique (EBSD) was used to identify the orientations of surface grains in the central zone of the fatigue specimen. Crack-initiation sites and slip systems associated to the initiated microcracks were identified. The criterion of the maximum Schmid Factor explains two thirds of the cracks initiated in slip systems, however if the favourably oriented slip band with respect to this criterion makes an angle of around 45° to the loading direction, a crack may initiate in another slip system.","palabras_clave":"Low -cycle Fatigue|Austenitic Stainless steels|In situ observations|EBSD|Crack initiation|Crystaline orientation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64530,"idioma_id":4,"titulo":"Epigenetic Modification Of Liver Mitochondrial Dna Is Associated With Histological Severity Of Nonalcoholic Fatty Liver Disease","resumen":"Objective & Design: Nonalcoholic fatty liver disease (NAFLD) is a clinical condition that refers to progressive histological changes ranging from simple steatosis (SS) to nonalcoholic steatohepatitis (NASH). We evaluated the status of cytosine methylation (5mC) of liver mitochondrial DNA (mtDNA) in selected regions of the mtDNA genome, such as D-loop control region, and mitochondrially encoded NADH dehydrogenase 6 (MT-ND6) and cytochrome C oxidase I (MT-CO1), to contrast the hypothesis that epigenetic modifications play a role in the phenotypic switching from SS to NASH. Methods: we studied liver biopsies obtained from patients with NAFLD in a case-control design; 45 patients and 18 controls.                         Results: MT-ND6 methylation was higher in the liver of NASH than SS patients (pMT-ND6 methylated DNA\/unmethylated DNA ratio was significantly associated with NAFLD activity score (pMT-ND6 mRNA expression was significantly decreased in NASH patients (0.26±0.30) vs. SS (0.74±0.48), pMT-ND6 methylation in NASH group was inversely correlated with the level of regular physical activity (R=-0.54, pMT-CO1 were not associated with the disease severity. DNA (cytosine-5) methyltransferase 1 was significantly upregulated in NASH patients (pUltrastructural evaluation showed that NASH is associated with mitochondrial defects and abnormal peroxisome proliferation.                         Conclusion: Hepatic methylation and transcriptional activity of the MT-ND6 are associated with the histological severity of NAFLD. Epigenetic changes of mtDNA are potentially reversible by interventional programs, as physical activity could modulate the methylation status of MT-ND6.                       ","palabras_clave":"EPIGENETICS|MITOCHONDRIAL DYSFUNCTION|MT-ND6|NAFLD|DNA METHYLATION|NASH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64531,"idioma_id":4,"titulo":"Exploiting phase separation in monolithic La0.6Ca0.4MnO3 devices","resumen":"Devices based on mesas were fabricated from thin films of magnetically phase-separated  La0.6Ca0.4MnO3. Low-field magnetoresistance arises because the volume fraction of the  ferromagnetic metallic phase is large enough for percolation but small enough to permit magnetic  decoupling between each mesa and the underlying track. Magnetic domain walls in the antiparallel  mesa-track configuration possess a giant resistance-area product of (3?7)108Xm2. This figure  represents an 11 order-of-magnitude improvement with respect to the figure for cobalt. VC 2013 AIP  Publishing LLC. [http:\/\/dx.doi.org\/10.1063\/1.4818314]","palabras_clave":"devices|magnetoresistance|manganites|phase separation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64532,"idioma_id":4,"titulo":"Seasonal pattern of the coastal fish assemblage in Anegada Bay, Argentina","resumen":"We describe here the seasonal variation of the inshore fish assemblage of Anegada Bay, North Patagonia, Argentina. Three  areas were seasonally sampled from 2007 to 2009 by means of a gang of bottom gill nets. We found 21 coastal fish species, but  species richness and fish number and weight changed throughout the year. The six species classified as dominant have  national and regional value for artisanal and recreational fishing and were responsible for the seasonal variation in the  fish assemblage. Both cluster and non-metric multidimensional scaling analyses based on fish number and fish weight indicated  two major sample groups encompassing spring and summer (the warmer seasons) and autumn and winter (the colder  seasons). The fish assemblage had higher species richness, dominance and abundance during the warmer seasons than during  the colder seasons in the same years and at the same sites. Water temperature was the main environmental factor structuring  the fish assemblage in Anegada Bay. We suggest that partial breeding migration toward the bay during warmer months could  explain the seasonal pattern observed. Nevertheless, variation in temperature conditions agreed well with the pattern of seasonal  changes, leading to an interaction between abiotic and biotic influences in determining the variability in this seasonal  fish assemblage. We conclude that an understanding of species temporal and spatial patterns in areas of high ecologic and  economic value, as exemplified by Anegada Bay, are essential for the implementation of a management approach oriented  toward ecosystem sustainability.","palabras_clave":"SEASONAL VARIATION|COASTAL SPECIES|SPATIO-TEMPORAL DYNAMICS|FISH ASSEMBLAGES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64533,"idioma_id":4,"titulo":"Effects of stocking density and natural food availability on the extensive cage culture of pejerrey (Odontesthes bonariensis) in a shallow Pampean lake in Argentina","resumen":"An experiment was conducted for 80 days at La Salada de Monasterio Lake (Buenos Aires, Argentina) to assess the effect of stocking density  and natural food availability on the growth and production of zooplanktivorous juveniles of pejerrey (Odontesthes bonariensis) in extensive cage culture. Ten cages were installed and stocked with 33-day-old fish, at three density treatments: 25, 50 and 75 ind. m3. Zooplankton was analysed in terms of abundance, biomass and diversity considering three size classes. Caged pejerrey diet was assessed regularly. The pejerrey gut contents composition was clearly different from cage zoo-  plankton, showing a trend to contain bigger components. Reared fish exhibited a tendency to diversification of the diet and to change the  mean prey size depending on fish length and stocking density. Regression models showed a positive and direct effect of the bigger zooplankton biomass on fish growth rates, which were also inversely affected by the availability of smaller zooplankton. The results demonstrate that  stocking density and available zooplankton, in both quantity and quality, are key factors in regulating extensive cage culture of pejerrey.  Handling stocking densities in a dynamic way it is possible to maximize fish growth, biomass harvest or fish number according to the goals of production","palabras_clave":"cage culture|pejerrey|stocking density|zooplankton quality"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64534,"idioma_id":4,"titulo":"Nectar and Flower Traits of Different Onion Male Sterile Lines Related to Pollination Efficiency and Seed Yield of F1 Hybrids","resumen":"Honey bees are the main pollinators of onion crops for seed production, but owing to low attractiveness of flowers, pollination is often inadequate. Pollination problems result in low seed yields. This problem is accentuated when male sterile lines (MSL) are used to produce hybrid onion seeds. In this study, the effect of floral attributes and nectar composition on the preference of honey bees of four MSLs and one onion open pollinated cultivar were assessed. The chemical composition of nectar was described through the analysis of sugars, trace elements, volatile organic compounds, and phenol compounds. The samples studied showed qualitative and quantitative differences in the analyzed traits of flowers and nectar among the different lines. Furthermore, field observations showed a great difference on the number of bee visits and seed yield among the onion lines analyzed. For the first time, this study demonstrates that there are marked differences in the chemical composition of nectar and floral morphology between open pollinated and MSLs and also within MSLs. In addition, these differences were correlated with the number of visits and seed yield. Therefore, it would be possible to select indirectly the most promising productive MSL using simple determinations of chemical compounds or floral morphological characters. ","palabras_clave":"Onion|Male Sterility|Nectar|seed production"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64535,"idioma_id":4,"titulo":"Molecular mapping of vernalization requirement and fertility restoration genes in carrot","resumen":"Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar riolla INTA and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot &nbsp;genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding. ","palabras_clave":"CARROT|VERNALIZATION|FERTILITY RESTORATION|MAPPING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64536,"idioma_id":4,"titulo":"Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size","resumen":"Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It is necessary to adjust the concentration and the length of treatment of the doubling agent. In this case, the effect of 250 and 500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L-1 amiprophos-methyl during 24 and 48 h was assessed over the rate of onion haploid plantlets chromosome doubling. The best duplication treatment was 250 mg.L-1 colchicine for 48 h, which yielded 100% of doubled haploid plants. On the other hand, a positive correlation resulted from the ploidy level and stomatal size, and a negative correlation between the level of ploidy and stomatal density. Significant differences between the stomatal length, width and density in haploid and doubled haploid plantlets were observed. An economical and quick method to test ploidy level in onion plantlets is proposed through the measurement of stomatal size and density.","palabras_clave":"Allium cepa L|Duplication; |Gynogenesis|Antimitotic agents"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64537,"idioma_id":4,"titulo":"A Petri Net approach for representing Orthogonal Variability Models","resumen":"The software product line (SPL) paradigm is used for developing software system products from a set of reusable artifacts, known as platform. The Orthogonal Variability Modeling (OVM) is a technique for representing and managing the variability and composition of those artifacts for deriving products in the SPL. Nevertheless, OVM does not support the formal analysis of the models. For example, the detection of dead artifacts (i.e., artifcats that cannot be included in any product) is an exhaustive activity which implies the verification of relationships between artifacs, artifacts parents, and so on. In this work, we introduce a Petri nets approach for representing and analyzing OVM models. The proposed net is built from elemental topologies that represents OVM concepts and relationships. Finally, we simulate the net and study their properties in order to avoid the product feasibility problems. ","palabras_clave":"SOFTWARE PRODUCT LINE|ORTHOGONAL VARIABILITY MODEL|PETRI NETS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64538,"idioma_id":4,"titulo":"Reasoning and Reuse in Software Architecture Design: Practices in the Argentine Industry","resumen":"Over the last years, software architecture design has gained significant importance in both, industrial and research areas due to its relevance in the software system development process. In this context, special attention has been given to the documentation of architects? reasoning during an architectural design, highlighting the advantages and disadvantages of this activity. This work intends to present a view of architects? practices in the Argentine industry regarding reasoning documentation and its subsequent use and access.","palabras_clave":"SURVEY|ARCHITECTURE|SOFTWARE|REASONING|REUSE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64540,"idioma_id":4,"titulo":"Experimental evidence and modeling of two types of electron traps in Al2O3 for nonvolatile memory  applications.","resumen":"Al2O3-based dielectrics are currently considered as promising materials to use in nonvolatile memories. The electron trap density in this material is much higher than in conventional SiO2, being their characteristics critical for the application. Conventional capacitance-voltage (C-V) techniques were used to study the main effects of the electron traps on the electrical characteristics of MOS capacitors with atomic layer deposited Al2O3 as insulating layer. More detailed information about the trapping kinetics was obtained through the study of the constant capacitance voltage transient. Two different types of traps were found. One is responsible for the instabilities observed in C-V measurements, the other has characteristic trapping times three orders longer. A physical model is presented to explain the observed trapping kinetics exhibiting good agreement between experiments and simulations. The energy levels of the studied traps were determined at 2.2 and 2.6&#8201;eV below the Al2O3 conduction band, with densities of 2.9&#8201;×&#8201;1018&#8201;cm&#8722;3 and 1.6&#8201;×&#8201;1018&#8201;cm&#8722;3, respectively.","palabras_clave":"ALUMINIUM COMPOUNDS|ATOMIC LAYER DEPOSITION|CONDUCTION BANDS|ELECTRON DENSITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64541,"idioma_id":4,"titulo":"The microbial food web structure of a hypertrophic warm temperate shallow lake, as affected by contrasting zooplankton assemblages","resumen":"The zooplankton of the hypertrophic Laguna Chascomús (Argentina) is generally  dominated by small rotifers and highly evasive cyclopoids. An unusual dominance by  small cladocerans was observed after a massive winter fish kill in 2007. The aim of this  study was to investigate the structure of the microbial food web under two contrasting  zooplankton assemblages. The lake was sampled every other week between 2007 and  2009. The abundances of heterotrophic bacteria (HB) and picocianobacteria (Pcy) rank  among the highest values reported for aquatic systems (up to 1.1 x 108 and 2.7 x 107  cells mL-1, respectively). Pcy averaged 53% of the total picoplanktonic biomass. The  abundance of ciliates displayed little varibility. HF were very abundant (>105 cells mL-  1) when small-cladocerans dominated, and decreased when rotifers dominated.  Picoplankton showed the opposite pattern, while the proportion of grazing resistant  morphologies (i.e. microaggregates of Pcy) was higher during the first summer. Our  data are consistent with empirical models describing the relationship between HF and  HB abundances. The HF-HB and HF-Pcy relationship was affected by changes in  zooplankton dominance. Contrarily to our hypothesis, the present results suggest that  large numbers of rotifers (>5000 ind. L-1) are more efficient than small-cladocerans at  controlling HF populations.","palabras_clave":"HYPERTROPHIC|SHALLOW LAKES|TROPHIC COUPLING|MICROBIAL FOOD WEB"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64542,"idioma_id":4,"titulo":"The plankton communities from peat bog pools:  Structure, temporal variation and environmental factors.","resumen":"This is the first characterization of the structure and temporal variation of the plankton communities comprising the complete food web in five peat bog pools related to environmental factors over two consecutive ice-free periods in Tierra del Fuego (548S). Remarkably, picophytoplankton was composed solely of eukaryotic cells, surpassing the dominance expectations for these acidic water bodies, whereas testaceans were virtually absent, even as tychoplankters. Abundances of the different planktonic communities were slightly higher than those reported for Northern Hemisphere peat bogs and humic lakes. Mixotrophic nutrition prevailed among nano- and microphytoplankters, a strategy also common in humic lakes. The structures in spring of the planktonic communities were similar. In contrast, in late summer there were differences in the abundance and biomass of the different trophic compartments among small, shallow water bodies and large ones. These seem to be dictated by distinct pool size-driven patterns of water temperature variation. A general shift in the control of heterotrophic flagellates abundance in the pools occurred, changing from bottom-up regulation in spring to top-down control in late summer related to temperature-dependant variations in zooplankton abundance and composition. We hypothesize that changes in the trophic interactions affecting the entire food web occur over the open water period in these aquatic ecosystems, and that these are dictated by pool morphometry and related abiotic features.","palabras_clave":"PEAT BOGS|FOOD WEB|POOLS|PLANKTON|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64543,"idioma_id":4,"titulo":"Regulation of the Cardiac Sodium\/Bicarbonate Cotransporter by Angiotensin II: Potential Contribution to Structural, Ionic and Electrophysiological Myocardial Remodelling","resumen":"The sodium\/ bicarbonate cotransporter (NBC) is, with the Na+\/H+ exchanger (NHE), an important alkalinizingmechanism that maintains cellular intracellular pH (pHi). In the heart exists at least three isoforms of NBC, one that promotesthe co-influx of 1 molecule of Na+ per 1molecule of HCO3-(electroneutral isoform; nNBC) and two others that generatesthe co-influx of 1 molecule of Na+ per 2 molecules of HCO3- (electrogenic isoforms; eNBC). In addition, the eNBCgenerates an anionic repolarizing current that modulate the cardiac action potential (CAP), adding to such isoforms therelevance to modulate the electrophysiological function of the heart. Angiotensin II (Ang II) is one of the main hormonesthat regulate cardiac physiology. The alkalinizing mechanisms (NHE and NBC) are stimulated by Ang II, increasing pHiand intracellular Na+ concentration, which indirectly, due to the stimulation of the Na+\/Ca2+ exchanger (NCX) operating inthe reverse form, leads to an increase in the intracellular Ca2+ concentration. Interestingly, it has been shown that Ang IIexhibits an opposite effect on NBC isoforms: it activates the nNBC and inhibits the eNBC. This inhibition generates aCAP prolongation, which could directly increase the intracellular Ca2+ concentration. The regulation of the intracellularNa+ and Ca2+ concentrations is crucial for the cardiac cellular physiology, but these ions are also involved in the developmentof cardiac hypertrophy and the damage produced by ischemia-reperfusion, suggesting a potential role of NBC incardiac diseases.","palabras_clave":"NBC|angiotensin|hypertrophy|heart"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64544,"idioma_id":4,"titulo":"Selective Binding of Albumin to GM1 Ganglioside Micelles Containing Paclitaxel","resumen":"In our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel(Dtx)) can be spontaneously loaded into monosialoganglioside (GM1) nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer. Here, we describe the hydrophobic interaction of Human Serum Albumin (HSA) with GM1 micelles loaded with Ptx, as a strategy that could improve tumour drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1\/Ptx\/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays showed that the nano-structures developed are taken up by cell cultures with an antimitotic activity of Ptx on tumoral and nontumoral cell lines that was similar to that observed with the free drug in DMSO solution.","palabras_clave":"Gangliosides|Paclitaxel|Albumin|Micelles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64545,"idioma_id":5,"titulo":"Los Estudios de Público Escolar y la Comunicación de la Arqueología","resumen":"En este trabajo se analizan los intereses, hábitos y valoraciones del público escolar asistente a la exposición ?Olavarría antes de Olavarría? realizada por un grupo de investigación. Esta clase de estudios, en general utilizada por museos, permite evaluar y (re)formular las prácticas de comunicación de la arqueología. Para ello se empleó una metodología cuali-cuantitativa y las variables seleccionadas fueron de tipo social, de opinión y valorativas, para lo que se requirió de encuestas, entrevistas, observaciones y registros durante las visitas guiadas de las escuelas. Entre los resultados más importantes del estudio se puede mencionar la buena recepción de la propuesta por parte de ambos grupos, con la diferencia que el público adolescente es más crítico y exigente. Por su parte los\/as docentes señalan dos aspectos positivos de la exhibición, primero su utilización como estrategia didáctica disparadora para continuar en el trabajo áulico y en segundo lugar, la posibilidad de interiorizarse sobre conocimientos que no estaban accesibles.   Por otro lado, esta exposición, al ser entendida en su rol educativo, social y comunicacional, también fue analizada dentro del marco de la llamada evaluación de exposiciones y este análisis permitió remarcar sus aspectos positivos y algunas cuestiones que tuvieron que modificarse durante su marcha. Finalmente, se considera importante generar propuestas al amplio público como compromiso universitario de devolución social, sin embargo, no dejan de ser activaciones patrimoniales dirigidas que no implican necesariamente apropiación y\/o valoración comunitaria.","palabras_clave":"Comunicación de la arqueología|Estudios de Público|Exposición|Docentes-estudiantes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64546,"idioma_id":4,"titulo":"Late Cretaceous dinosaurs from the James Ross Basin, West Antarctica","resumen":"The fossil record of terrestrial vertebrates from the Late Cretaceous of Antarctica is currently composed of non-avian and avian dinosaurs from the marine sediments of the James RossBasin, Antarctic Peninsula (West Antarctica). Although two dinosaurian formational assemblages (Late Campanian\/Early Maastrichtian and Late Maastrichtian) are known, the record is still scattered,and evolutionary scenarios are tentative. Ten non-avian dinosaurs have been reported from Coniacian to Maastrichtian deposits, along with possible sauropod footprints of Early Maastrichtianage from Snow Hill Island. Five avian dinosaurs have been recorded or described exclusively from the Maastrichtian. The presence of an advanced titanosaur with characteristic procoelous mid-caudal vertebrae in Snow Hill Island Formation at Santa Marta Cove implies that the groupachieved a global distribution by the Late Campanian. The Late Campanian\/Early Maastrichtian non-avian dinosaur (ankylosaurs, ornithopods and dromaeosaurid theropods) clades probably attained a near-cosmopolitan distribution before the Late Cretaceous, and some aspects of this hallmark \u2018Gondwanan\u2019 fauna may therefore reflect climate-driven provinciality, not vicariant evolution driven by continental fragmentation. Antarctic Late Cretaceous avian dinosaurs are rare.They are restricted to the Maastrichtian and consist of a cariamid?, gaviids, a charadriiform and the basal Anseriformes Vegavis, and provide the first strong evidence for a basal radiation of birds known to exist in the Cretaceous.","palabras_clave":"West Antarctica.  |Late Cretaceous|Non-avian and avian dinosaurs. |Palaeobiogeography|James Ross Basin."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64547,"idioma_id":5,"titulo":"Colección osteológica de aves actuales de la División Paleontología Vertebrados del Museo de La Plata","resumen":"En el Museo de La Plata, la Colección Osteológica de Aves actuales de la  División Paleontología Vertebrados surgió para dar respuesta a las demandas de los paleontólogos que requieren material osteológico Desarticulado para los estudios de comparación anatómica. De esta forma, el material óseo podía ser analizado por piezas independientes, siendo factible su estudio y comparación con diversas metodologías comparativas. Una colección de este tipo no presenta las limitaciones de manejo de los  esqueletos articulados y montados que habitualmente se conservan para la exhibición. A noviembre de 2012, posee un total de 743 ejemplares, 225 son esqueletos completos, 355 poseen procedencia geográfica, 79 son ejemplares provenientes del Jardín Zoológico de La Plata, Estación Biológica de Aves Silvestres (EBAS), Estación de Cría de Animales  Silvestres (ECAS) y criaderos de la zona, y 25 son el producto de intercambio con instituciones extranjeras. Para facilitar la comparación de materiales, particularmente huesos aislados o fragmentos con los Cuales comúnmente trabajan paleontólogos, zooarqueólogos y  ornitólogos interesados en aspectos osteológicos, se conformó una colección de huesos homólogos compuesta por el cráneo, cinturas y elementos derechos de cada miembro de una especie representativa de cada familia. Colecciones de esta naturaleza son importantes en la  resolución de aspectos sistemáticos primarios, anatómicos, evolutivos y morfométricos de las aves, tanto en el campo neontológico como en el palentológico. Asimismo, los inventarios son de utilidad en tanto revelan deficiencias y fortalezas de las colecciones que permiten la planificación de su crecimiento para futuras investigaciones.","palabras_clave":"AVES|OSTEOLOGIA|COLECCION|MUSEO DE LA PLATA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64548,"idioma_id":4,"titulo":"Food web structure in a double-basin ultra-oligotrophic lake in Northwest Patagonia, Argentina, using carbon and nitrogen stable isotopes","resumen":"Fish, benthic macroinvertebrates, macrophytes, biofilm, plankton, and terrestrial material were analyzed for stable nitrogen (15N) and carbon isotopes (13C) to study the food web structure of a double-basined, ultra-oligotrophic lake in northwest Patagonia. The 15N values of all biota were consistently higher in Moreno East basin, probably caused by a higher anthropogenic impact; however, the estimated trophic positions of fish showed no differences between basins, indicating that fish are feeding from food chains with different 15N baselines. Fish 13C values were higher in Moreno West basin but no pattern was observed for the rest of the organisms analyzed. The different hydrogeomorphic characteristics between the basins could lead to these differences in the C signatures of fish. SIAR (Stable Isotope Analysis in R) mixing model was used to study possible food sources for adult fish and the results were contrasted with published gut content analysis. According to the model, in both basins small size fish such as Small Puyen (Galaxias maculatus) and juvenile fish, had the highest relative isotopic contribution to introduced RainbowTrout (Oncorhynchus mykiss) and Brook Trout (Salvelinus fontinalis), which had lower contributions from other sources such as insect larvae, decapods, and snails. On the contrary, decapods and insect larvae were isotopically more important for the native Creole Perch (Percichthys trucha) than small fish in both basins. An ontogenetic shift in the carbon source and trophic level was observed in Puyen Chico and Rainbow Trout from the West basin.","palabras_clave":"FOOD WEBS|CARBON STABLE ISOTOPES|NITROGEN STABLE ISOTOPES|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64549,"idioma_id":4,"titulo":"Feeding strategy of the exotic Rainbow trout (Oncorhynchus mykiss) at low order Patagonian streams","resumen":"During 2005-2006 we studied the diet composition and the feeding strategy of the Rainbowtrout (Oncorhynchus mykiss) at two low order Patagonian streams. Benthic Macroinvertebrate&nbsp; availability was estimated at both riffles and pools. Fish stomach contents were examined to&nbsp; determine prey richness and diversity, prey selectivity, food-niche width, and the feeding&nbsp; strategy employed by trouts along the year. Benthos availability varied seasonally, Ephemeroptera, Trichoptera, Plecoptera and Diptera species dominated in samples. Rainbow trout diet was composed mainly by benthic macroinvertebrates, followed by terrestrial insects, fish, algae and plants. Different trout size-classes segregated the use of food resources to reduce the predation pressure. Selected preys included organisms displaying none to high mobility. A high feeding plasticity allows trout to buffer changes in food availability by switching from a specialized to a generalized feeding behavior. Consequently, trout may opportunistically exploit abundant but temporary food resources, which would explain their marked expansion in Patagonia environments.","palabras_clave":"BENTHOS AVAILABILITY|DIET COMPOSITION|EXOTIC TROUTS|FEEDING STRATEGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64550,"idioma_id":4,"titulo":"MODULATION OF BIOTRANSFORMATION SYSTEMS AND ABC TRANSPORTERS BY BENZNIDAZOLE IN THE RAT","resumen":"The effect of antichagasic benznidazole (BZL; 100 mg\/kg body weight\/day, 3 consecutive days, intraperitoneally) on biotransformation systems and ABC transporters was evaluated in rats. Expression of cytochrome P-450 (CYP3A), UDP-glucuronosyltransferase (UGT1A), glutathione S-transferases (glutathione S-transferase alpha, mu and pi), multidrug-resistance- associated protein 2 (Mrp2), and P glycoprotein (P-gp) in liver, small intestine, and kidney was estimated by Western blotting. Increases in hepatic CYP3A (30%) and GSTmu (40%) and in intestinal GSTalpha (72% in jejunum and 136% in ileum) were detected. Significant increases in Mrp2 (300%) and P-gp (500%) proteins in liver from BZL-treated rats were observed without changes in kidney. P-gp and Mrp2 were also increased by BZL in jejunum (170% and 120%, respectively). In ileum, only P-gp was increased by BZL (50%). The activities of GST, P-gp, and Mrp2 correlated well with the upregulation of proteins in liver and jejunum. Plasma decay of a test dose of BZL (5 mg\/kg body weight) administered intraduodenally was faster (295%) and the area under the concentration-time curve (AUC) was lower (41%) for BZL-pretreated rats than for controls. The biliary excretion of BZL was higher (60%) in the BZL group, and urinary excretion of BZL did not show differences between groups. The amount of absorbed BZL in intestinal sacs was lower (25%) in pretreated rats than in controls. In conclusion, induction of biotransformation enzymes and\/or transporters by BZL could increase the clearance and\/or decrease the intestinal absorption of coadministered drugs that are substrates of these systems, including BZL itself.","palabras_clave":"BENZNIDAZOLE|BIOTRANSFORMATION SYSTEMS|ABC TRANSPORTERS|DISPOSITION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64551,"idioma_id":4,"titulo":"The role of pH fronts in tissue electroporation based treatments","resumen":"Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss.","palabras_clave":"TUMOR TREATMENT|REVERSIBLE ELECTROPORATION|PH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64552,"idioma_id":4,"titulo":". Epibiotic relationships on Zygochlamys patagonica (Mollusca, Bivalvia, Pectinidae) increase biodiversity in a submarine canyon in Argentina.","resumen":"The continentalslopeofthesouthernSWAtlanticOceanhasmanydistinguishabledeepsubmarine canyons,varyingindepthandextension.Thebenthicfaunawithinoneofthem,detectedinApril2005 by meansofamultibeamSIMRADEM1002sonar,andlocatedat43135??S to59133??W,325mdepth,was studied todiscussfaunalaffinities withtheneighbouringPatagonianscallop fishing groundslocatedat upper slopedepths.Inordertoaddfaunalinformationtothepreviousgeneralstudy,westudiedthe epibiotic speciessettledonPatagonianscallops(thedominantspeciesinthearea)collectedinthe referencesamplingsiteusinga2.5-mmouth-openingdredge,10mmmeshsize.Wesampled 103scallopswithshellheightsbetween22and69mm;epibiontswererecordedonbothvalves.We found 53epibiotictaxa,whichweremostconspicuousontheuppervalve.Bryozoawasthemostdiverse group (34species)whilePolychaetawasthemostabundantgroup,recordedon94%ofthescallops. Stylasteridae(2species)andClavulariidae(Cnidaria)conformnewlyrecordedepibiontson Z. patagonica and thesponge Tedania (Tedaniopsis) infundibuliformis also representsanewrecordfortheSW Atlantic Ocean. ","palabras_clave":"SUBMARINE CANYON|EPIBIOTIC ASSOCIATIONS|BIODIVERSITY|ZYGOCHLAMYS PATAGONICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64553,"idioma_id":4,"titulo":"In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp","resumen":"tPaenibacillus larvae, a sporulating Gram-positive bacterium, is the etiological agent of American foulbrooddisease in Apis mellifera L. Plant extracts could be a natural alternative to control this pathology. Thecurrent study assessed the anti-P. larvae effect of extracts and pure principal products from the Flourensiagenus: F. riparia, F. fiebrigii and F. tortuosa. Their inhibitory effect was assayed against different P. larvaestrains according to the disk diffusion technique and subsequently, the minimal inhibitory concentrations(MIC) of extracts by the agar dilution method was determined. Furthermore, toxicity of the most effectiveextracts against P. larvae was tested in bees. All extracts inhibited growth of the different P. larvae strainsassayed. However, the magnitude of the antagonistic effect depended on the chemical nature of theextract and the P. larvae strain. Chloroform extracts (CE) and ethyl ether extracts (ETE) from F. ripariaand ETE from F. fiebrigii were most active against P. larvae Azul, the most sensitive indicator strain withMIC values of 250 ppm (CE) and 2000 ppm (ETE) for F. riparia, and 2500 ppm (ETE) for F. fiebrigii. Hexaneextracts from the three species did not present any significant inhibitory effect. These results wouldindicate that one or some of the more polar compounds would cause inhibition of this pathogen. Toxicityassays demonstrated that even the highest concentrations assayed (125,000 ppm) did not show lethaleffects on exposed bees during in vitro conditions.Paenibacillus larvae, a sporulating Gram-positive bacterium, is the etiological agent of American foulbrooddisease in Apis mellifera L. Plant extracts could be a natural alternative to control this pathology. Thecurrent study assessed the anti-P. larvae effect of extracts and pure principal products from the Flourensiagenus: F. riparia, F. fiebrigii and F. tortuosa. Their inhibitory effect was assayed against different P. larvaestrains according to the disk diffusion technique and subsequently, the minimal inhibitory concentrations(MIC) of extracts by the agar dilution method was determined. Furthermore, toxicity of the most effectiveextracts against P. larvae was tested in bees. All extracts inhibited growth of the different P. larvae strainsassayed. However, the magnitude of the antagonistic effect depended on the chemical nature of theextract and the P. larvae strain. Chloroform extracts (CE) and ethyl ether extracts (ETE) from F. ripariaand ETE from F. fiebrigii were most active against P. larvae Azul, the most sensitive indicator strain withMIC values of 250 ppm (CE) and 2000 ppm (ETE) for F. riparia, and 2500 ppm (ETE) for F. fiebrigii. Hexaneextracts from the three species did not present any significant inhibitory effect. These results wouldindicate that one or some of the more polar compounds would cause inhibition of this pathogen. Toxicityassays demonstrated that even the highest concentrations assayed (125,000 ppm) did not show lethaleffects on exposed bees during in vitro conditions.","palabras_clave":"PAENIBACILLUS LARVAE|HONEYBEE|FLOURENCIA RIPARIA|FLOURENCIA FIEBRIGII|PLANT EXTRACTS|ANTIMICROBIAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64554,"idioma_id":4,"titulo":"Late Holocene armadillos (Mammalia, Dasypodidae) of the Sierras of Córdoba, Argentina: Zooarchaeology, diagnostic characters and their paleozoological relevance","resumen":"The results of zooarchaeological study carried out on the bone remains of armadillos (Mammalia,Dasypodidae) from four Sierras of Córdoba (Argentina) archaeological sites were presented. The radiocarbondates place chronologically their occupation at the last millennium of the Late Holocene (ca.970  110 and 290  37 14C BP). Four taxa were identified: Chaetophractus villosus, Chaetophractus vellerosus,Euphractus sp. and Dasypus sp. The diagnostic characters of their bony dermal scutes or osteodermswere described in order to facilitate their taxonomical identification in other modern collectionand\/or fossil assemblages. C. villosus and C. vellerosus show a current distribution in the study area, whileEuphractus sp. and Dasypus sp. are absent in the Sierras of Córdoba. Euphractus was determined on thebasis of a fragmented movable osteoderm in C.Pun.39. Their presence may be a response to an increase ofprecipitation and temperature associated with the ?Medieval Warm Period?. The record of Dasypus sp.during the ?Little Ice Age? contrasts negatively with its potential as an indicator of warm and humidcondition, showing either their adaptive plasticity or the biological fragmentation of the mountainenvironment under paleoclimatic conditions. Finally, the possibility that the presence of Euphractus andDasypus is due to anthropogenic transport was considered.","palabras_clave":"ARCHAEOFAUNA|LITTLE ICE AGE|MEDIEVAL WARM PERIOD|XENARTHRA|OSTEODERM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64555,"idioma_id":5,"titulo":"Prácticas Resistentes, Elusión y Reproducción Social en un Contexto Histórico Adverso. Una Mirada a los Indígenas de Córdoba (Argentina) en Tiempos Coloniales Tempranos (siglos XVI y XVII)","resumen":"        El presente artículo trata sobre los indígenas de Córdoba (Argentina) y sus estrategias de reproducción social en un contexto histórico adverso, tras la implantación del régimen colonial español a fines del siglo XVI. La investigación se desarrolla en el marco de la arqueología histórica, concebida como el estudio del proceso de modernización que siguió a la expansión europea a escala global y sus efectos sobre innumerables sociedades locales. Se analiza el caso de un sitio arqueológico concreto, el Alero Tala Huasi, ocupado desde momentos previos a la conquista española hasta el Período Colonial Temprano, entre fines del siglo XVI y la primera mitad del XVII. La persistencia de prácticas económicas, tecnológicas y rituales de raíces prehispánicas, así como la incorporación selectiva de elementos foráneos, como el ganado euroasiático consumido en el lugar, son interpretadas en clave de resistencia y elusión de las relaciones impuestas de dominación, en un intento por mantener y reproducir la integración grupal. Se destaca la potencialidad de la arqueología histórica para indagar sobre procesos sociales al margen de los registros escritos, al entender aspectos de la vida cotidiana y de la agencia de colectivos sojuzgados e invisibles en el discurso oficial de la sociedad colonial.                           ","palabras_clave":"RESISTENCIA INDIGENA|CONTEXTOS RITUALES|PRACTICAS TRADICIONALES|ROBO DE GANADO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64556,"idioma_id":5,"titulo":"Geología de la Sierra de Chávez en el sector centro occidental de la Sierra de Valle Fértil, San Juan","resumen":"La geología de la Sierra de Chávez está constituida por rocas ígneas y metamórficas intercaladas complejamente a todas las escalas, y corresponderían a la unidad máfica definida en la sierra de Valle Fértil. Cinco asociaciones litológicas han sido identificadas en éste complejo: diorítica, gábrica, metasedimentaria, milonítica y granítica-pegmatítica. Las estimaciones termobarométricas realizadas en las migmatitas indican condiciones de equilibrio para el pico metamórfico (M) 1de 876 °C y 8,3 Kbar y reflejarían el efecto del magmatismo durante el Ordovícico Temprano (489 - 470 Ma). Las presiones aquí estimadas corresponden a las mayores paleo-profundidades reconocidas en todo el arco magmático Famatiniano. Las relaciones de campo y el análisis estructural permitieron establecer la presencia de varias fábricas magmáticas y tectónicas, las cuales son: i) estructuras magmáticas (S0) interpretadas como una foliación magmática primaria, ii) foliación submagmática a subsólida de alta temperatura y foliación metamórfica (S1), iii) foliación milonítica estabilizada en facies de anfibolita (S2a) y esquistos verdes (S2b) y iv) foliación frágil (S3) definida como un clivaje de fractura centimétrico. (S0) y (S1) están asociadas al primer estadío de deformación (D1) reconocido en la zona, desarrollado en condiciones de alta temperatura (facies de granulita) y coetáneo con el magmatismo y el pico metamórfico (M1). (S2a,b) y (S3) transponen a las estructuras ígneas y metamórficas generadas durante el evento D1, y se asocian a un segundo y tercer estadío de deformación (D2-D3), representando una evolución progresiva de la deformación desde dominios dúctiles a frágiles, vinculadas a las etapas de cierre y exhumación del arco Famatiniano.","palabras_clave":"Sierra de Chávez|Arco Famatiniano|Complejo máfico|termobarometría"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64557,"idioma_id":4,"titulo":"Malignancy Risk Models  for  Oral Lesions","resumen":"OBJECTIVES:The aim of this work was to assess risk habits, clinical and cellular phenotypes and TP53 DNA changes in oral mucosa samples from patients with Oral Potentially Malignant Disorders (OPMD), in order to create models that enable genotypic and phenotypic patterns to be obtained that determine the risk of lesions becoming malignant.STUDY DESIGN:Clinical phenotypes, family history of cancer and risk habits were collected in clinical histories. TP53 gene mutation and morphometric-morphological features were studied, and multivariate models were applied. Three groups were estabished: a) oral cancer (OC) group (n=10), b) oral potentially malignant disorders group (n=10), and c) control group (n=8).RESULTS:An average of 50% of patients with malignancy were found to have smoking and drinking habits. A high percentage of TP53 mutations were observed in OC (30%) and OPMD (average 20%) lesions (p=0.000). The majority of these mutations were GC TA transversion mutations (60%). However, patients with OC presented mutations in all the exons and introns studied. Highest diagnostic accuracy (p=0.0001) was observed when incorporating alcohol and tobacco habits variables with TP3 mutations.CONCLUSIONS:Our results prove to be statistically reliable, with parameter estimates that are nearly unbiased even for small sample sizes. Models 2 and 3 were the most accurate for assessing the risk of an OPMD becoming cancerous. However, in a public health context, model 3 is the most recommended because the characteristics considered are easier and less costly to evaluate.","palabras_clave":"TP53|OPMD|ORAL CANCER|public health"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64558,"idioma_id":4,"titulo":"Analysis of the Interaction Interfaces of the N-Terminal Domain from Pseudomonas aeruginosa MutL","resumen":"Mismatch Repair System corrects mutations arising from DNA replication that escape from DNA polymerase proofreading activity. This system consists of three main proteins, MutS-L-H, responsible for lesion recognition and repair. MutL is a member of GHKL ATPase family and its ATPase cycle has been proposed to modulate MutL activity during the repair process. Pseudomonas aeruginosa MutL (PaMutL) contains an N-terminal (NTD) ATPase domain connected by a linker to a Cterminal  (CTD) dimerization domain that possesses metal ion-dependent endonuclease activity. With the aim to identify characteristics that allow the PaMutL NTD allosteric control of CTD endonuclease activity, we used an in silico and experimental approach to determine the interaction surfaces of P. aeruginosa NTD (PaNTD), and compared it with the well characterized Escherichia coli MutL NTD (EcNTD). Molecular dynamics simulations of PaNTD and EcNTD bound to or free of adenosine nucleotides showed that a significant difference exists between the behavior of the EcNTD and PaNTD  dimerization interface, particularly in the ATP lid. Structure based simulations of MutL homologues with endonuclease activity were performed that allowed an insight of the dimerization interface behavior in this family of proteins. Our experimental results show that, unlike EcNTD, PaNTD is dimeric in presence of ADP. Simulations in mixed solvent allowed us to identify the PaNTD putative DNA binding patch and a putative interaction patch located opposite to the dimerization face. Structure based simulations of PaNTD dimer in presence of ADP or ATP suggest that nucleotide binding could differentially modulate PaNTD protein-protein interactions. Far western assays performed in presence of ADP or ATP are in agreement with our in silico analysis.","palabras_clave":"P. aeruginosa|MutL|protein-protein interaction|Bioinformatic"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64559,"idioma_id":4,"titulo":"Analysis of DNA structure and sequence requirements for Pseudomonas aeruginosa MutL endonuclease activity","resumen":"The hallmark of the mismatch repair system in bacterialand eukaryotic organisms devoid of MutH is thepresence of a MutL homologue with endonucleaseactivity. The aim of this study was to analyse whetherdifferent DNA structures affect Pseudomonas aeruginosaPseudomonas aeruginosa MutL (PaMutL) endonuclease activity and todetermine if a specific nucleotide sequence is requiredfor this activity. Our results showed that PaMutL wasable to nick covalently closed circular plasmids but notlinear DNA at high ionic strengths, while the activityon linear DNA was only found below 60mM salt. Inaddition, single strand DNA, ss\/ds DNA boundariesand negatively supercoiling degree were not requiredfor PaMutL nicking activity. Finally, the analysis ofthe incision sites revealed that PaMutL, as well asBacillus thuringiensis MutL homologue, did not show DNA sequence specificity.DNA sequence specificity.MutL homologue, did not show DNA sequence specificity.","palabras_clave":"DNA sequence or structure|endonuclease activity|mismatch repair|MutL|Pseudomonas aeruginosa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64560,"idioma_id":4,"titulo":"Modeling  the  complex  hatching  and  development  of  Aedes  aegypti  in temperate  climates","resumen":"Here, we present and discuss a compartmental stochastic model for Aedes aegypti conceived as a math-ematical structure able to interpolate and extrapolate (predict) biological phenomena, and direct theattention to biological matters that need experimental elucidation. The model incorporates weatherinformation in the form of daily temperatures and rain and pays particular attention to determiningfactors in temperate climates. Sufficiently large rains trigger egg hatching, which in turn leads to peaksin larval densities. Hatching is inhibited by the absence of bacteria (Gillett effect), a mechanism of rel-evance during the winter season and in seasons with isolated rains. The model also incorporates egghatching independent of rains. Both egg hatching and larval development depend on the availability offood, which is modeled as bacteria produced at rates that depend on the temperature. Larval mortalityand pupation rates depend on the larvae to bacteria ratio. The results of the model for egg laying activ-ity were compared with field records during a normal season and a drought. Both the model and therecords indicate that the egg laying activity of Ae. aegypti is not zero during the drought and recoversquickly when normal weather is reestablished. We studied the sensitivity of the model to different setsof physiological parameters published for a few different local populations of this species, and found thatthere is an important sensitivity to local characteristics that will affect some predictions of the model.We emphasize that if the information is going to be used to evaluate control methods, the life cycle of themosquito must be studied for the local strain under the local environmental conditions (including food).We showed that the adult populations produced by the model are insensitive to certain combinations ofparameters and that this insensitivity is related to the variability reported for different strains obtainedfrom closely located places. When the model is considered in a larger biological context, it indicates thatsome standard procedures performed to measure the life cycle of Ae. aegypti in the laboratory might havea determining influence in the results.","palabras_clave":"STOCHASTIC MODEL|FOOD|BACTERIA|LOCAL STRAINS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64561,"idioma_id":4,"titulo":"A mathematically assisted reconstruction of the initial focus of the yellow fever outbreak in Buenos Aires (1871)","resumen":"We discuss the historic mortality record corresponding to the initial focus of the yellowfever epidemic outbreak registered in Buenos Aires during the year 1871 as comparedto simulations of a stochastic population dynamics model. This model incorporates thebiology of the urban vector of yellow fever, the mosquito Aedes aegypti, the stages ofthe disease in the human being as well as the spatial extension of the epidemic outbreak.After introducing the historical context and the restrictions it puts on initial conditions andecological parameters, we discuss the general features of the simulation and the dependenceon initial conditions and available sites for breeding the vector. We discuss the sensitivity,to the free parameters, of statistical estimators such as: nal death toll, day of the yearwhen the outbreak reached half the total mortality and the normalized daily mortality,showing some striking regularities. The model is precise and accurate enough to discuss thetruthfulness of the presently accepted historic discussions of the epidemic causes, showingthat there are more likely scenarios for the historic facts.","palabras_clave":"EPIDEMIOLOGÍA|FIEBRE AMARILLA|MODELO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64562,"idioma_id":4,"titulo":"Effects of Thermal Heterogeneity and Egg Mortality on Differences in the Population Dynamics of Aedes aegypti (Diptera: Culicidae) Over Short Distances in Temperate Argentina","resumen":"In temperate regions, the seasonal dynamics of Aedes aegypti (L.) (Diptera: Culicidae) is mainly inßuenced by temperature, whereas the probability of local extinction depends on the egg mortality during the cold season. The aim of the current study was to assess the importance of temperature and egg mortality in determining the differences in the oviposition dynamics of Ae. aegypti between favorable and less favorable areas in Buenos Aires City (Argentina). Year-round temperature dynamics were monitored, and oviposition dynamics were experimentally studied with ovitraps at two sites. Daily egg mortality values were calculated from a previous study performed at the same sites. The relative contribution of the differences in temperature and egg mortality between sites to the oviposition dynamics was assessed by means of a mathematical stochastic population dynamics model for Ae. aegypti. The results showed higher temperature and lower daily egg mortality at the site where higher oviposition activity was recorded. A larger inßuence of temperature than of egg mortality on population abundance during most of the activity season was detected in the results of the simulations. Our results showed a temperature gradient that relates to the distance to the Rio de la Plata river and contributes to explaining the spatial heterogeneity in Ae. aegypti populationabundances previously reported. The hypothesis of local extinctions because of egg mortality during the winter was not supported by the present analysis. The differences between Þeld oviposition dynamics and simulation results suggest that rainfall might also be an important variable under extremely dry conditions.","palabras_clave":"SPATIAL DISTRIBUTION|OVIPOSITION|SEASONAL DYNAMICS|STOCHASTIC MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64563,"idioma_id":4,"titulo":"Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow's milk proteins.","resumen":"The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA- specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow´s Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the established Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.      ","palabras_clave":"BRUCELLA|ALLERGY|ADJUVANT|MODULATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64564,"idioma_id":4,"titulo":"Computational Chemical Analysis of Unconjugated Bilirubin Anions and Insights into pKa Values Clarification","resumen":"The pKa, the negative logarithm of the acid dissociation equilibrium constant, of the carboxylic acid groups of unconjugated bilirubin in water is a discussed issue because there are quite different experimental values reported. Using quantum mechanical calculations we have studied the conformational behavior of unconjugated bilirubin species (in gas phase and in solution modeled implicitly and explicitly) to provide evidence that may clarify pKa values because of its pathophysiological relevance. Our results show that rotation of carboxylate group, which is not restricted, settles it in a suitable place to establish stronger interactions that stabilizes the monoanion and the dianion to be properly solvated, demonstrating that the rationalization used to justify the high pKa values of unconjugated bilirubin is inappropriate. Furthermore, low unconjugated bilirubin (UCB) pKa values were estimated from a linear regression analysis.","palabras_clave":"BILIRUBIN|PKA|HIDROGEN BOND|CALCULATIONS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64565,"idioma_id":4,"titulo":"Sequence of Lactobacillus rhamnosus CRL1505, an Inmunobiotic Strain Used in Social Food Programs in Argentina","resumen":"We report the draft genome sequence of theprobiotic Lactobacillus rhamnosus strainCRL1505. This new probiotic strain has been included into official NutritionalPrograms in Argentina. The draft genome sequence is composed of 3,417,633 bpwith 3,327 coding sequences","palabras_clave":"BACTERIAS LACTICAS|GENOMA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64566,"idioma_id":4,"titulo":"Genome sequence of the cheese-starter strain Lactobacillus delbrueckii subsp. lactis CRL 581","resumen":"Lactobacillus delbrueckii subsp. lactis is a thermophilic lactic acid bacteria widely used as astarter culture for the manufacture of a variety of fermented dairy products.Here, we report the genome sequence of Lactobacillus delbrueckiisubsp. lactis CRL 581 (1,911,137bp, GC 49.7%) , a proteolytic strain isolated from ahome-made Argentinian hard cheese whichhas a key role in bacterial nutrition as well as it can release bioactivehealth-beneficial peptides from milk proteins","palabras_clave":"LACTOBACILLUS|GENOMA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64567,"idioma_id":4,"titulo":"Trypanosoma cruzi TcSMUG L surface mucins promote development and infectivity in the triatomine vector Rhodnius prolixus","resumen":"Background: TcSMUG L products were recently identified as novel mucin-type glycoconjugates restricted to the surface of      insect-dwelling epimastigote forms of Trypanosoma cruzi, the etiological agent of Chagas disease. The remarkable      conservation of their predicted mature N-terminal region, which is exposed to the extracellular milieu, suggests that      TcSMUG L products may be involved in structural and\/or functional aspects of the interaction with the insect vector.      Methodology and Principal Findings: Here, we investigated the putative roles of TcSMUG L mucins in both in vivo      development and ex vivo attachment of epimastigotes to the luminal surface of the digestive tract of Rhodnius prolixus. Our      results indicate that the exogenous addition of TcSMUG L N-terminal peptide, but not control T. cruzi mucin peptides, to the      infected bloodmeal inhibited the development of parasites in R. prolixus in a dose-dependent manner. Pre-incubation of      insect midguts with the TcSMUG L peptide impaired the ex vivo attachment of epimastigotes to the luminal surface      epithelium, likely by competing out TcSMUG L binding sites on the luminal surface of the posterior midgut, as revealed by      fluorescence microscopy.      Conclusion and Significance: Together, these observations indicate that TcSMUG L mucins are a determinant of both      adhesion of T. cruzi epimastigotes to the posterior midgut epithelial cells of the triatomine, and the infection of the insect      vector, R. prolixus.","palabras_clave":"Trypanosoma cruzi|Rhodnius prolixus|mucin|attachment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64568,"idioma_id":4,"titulo":"Cold-set gelation of high pressure-treated soybean proteins","resumen":"The ability of soybean proteins to form cold-set gels, using high pressure (HP) processing as denaturing agent and calcium incorporation was evaluated. Different protein preparations were assayed: soybean protein isolate (SPI), a b-conglycinin enriched fraction (7SEF) and a glycinin enriched fraction (11SEF). 7SEF formed aggregated gels with low water holding capacity whereas 11SEF did not form self standing gels. SPI formed the better gels: ordered and with high water holding capacity. SPI gels were relatively soft and their visual aspect, rheological and texture properties, and water holding capacity depended on HP level (400e600 MPa), CaCl2 concentration (0.015e0.020 mol L_1) and protein concentration (85e95 g L_1), thus gels with different characteristics may be obtained. The gels comprised a three dimensional network stabilized by non-covalent interactions with spaces filled of proteins in aqueous solution. The results indicate that it is possible to use HP and subsequent calcium incorporation to form self-standing cold-set gels from soybean proteins. These gels may be of interest to incorporate heat-labile compounds or probiotics during the gelation step.","palabras_clave":"Soybean proteins |Cold-set gelation |High pressure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64569,"idioma_id":4,"titulo":"Nutritional and anti-nutritional characterization of four cowpea varieties under thermal treatments: Canonical discriminant analysis","resumen":"In the Northeast of Argentina, cowpeas are frequently produced by small- and medium-sized farmers for  either personal consumption or trade. The objective of this work was to study, using a principal  component analysis (PCA), the effect of thermal and non-thermal treatments on the nutritional and antinutritional  properties of the four different varieties of Vigna unguiculata.  Anti-nutrients contents allowed treatment differentiation for each variety because they contributed  mainly in the PC1 axis construction (PCA). In contrast, nutrient (proteins and carbohydrates) contribution  to treatment differentiation was not significant. Colorado variety presented the highest mineral in vitro  availability, with the exception of iron. Thermal treatment increased the protein digestibility of all flours.  The typical thermograms of native flours presented a two overlapping endotherm with denaturing  temperatures (Td) of 83e84 _C and 91e93 _C respectively and a total enthalpy (DH) 7e9 J\/g. Heat  treatments produced a shift of Td (67e73 _C) with increasing treatment time.  Cowpea seeds varieties, especially Colorado and Cuarenton, because of their nutritional and antinutritional  components under thermal treatments, could be used as an alternative low cost protein  source for food formulas.","palabras_clave":"CHEMICAL COMPOSITION|TANNINS|PHYTIC ACID|THERMAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64570,"idioma_id":4,"titulo":"Amaranth protein films from thermally treated proteins","resumen":"ver adjunto","palabras_clave":"Protein films|Amaranth proteins|Thermal treatment|Protein cross-linking|Mechanical properties"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64571,"idioma_id":4,"titulo":"Interaction of modified celluloses and pectins with gluten proteins","resumen":"Physical and chemical techniques were applied to characterize the type of interaction between hydrocolloids  and the gluten network in wheat dough, with and without NaCl. Modified celluloses (microcrystalline  cellulose, MCC; carboxymethylcellulose, CMC, hydroxypropylmethylcelluloses, HPMC) and  pectins of low (LMP) and high (HMP) degree of methylation were utilized as hydrocolloids to interact  with gluten proteins. Modified celluloses were employed at 1.5% (flour basis) and pectins at 2.0% (flour  basis). By microscopy (SEM and CLSM), it could be observed that NaCl induced a more marked crosslinking  and orientation of gluten network. On the other hand, the addition of hydrocolloids led to more  open matrices. Molecular mobility was evaluated by 1H-NMR assays and significant effects of NaCl  addition and hydrocolloid type were found on relaxation times (T2). In presence of salt, significantly  higher relaxation times were observed when modified celluloses were added. Hydrocolloid addition  strongly affected the secondary conformation of proteins as studied by FT-Raman. In absence of NaCl,  control and MCC samples exhibited the higher a-helix conformation percentage (indicating a more ordered  and compact structure), followed by dough with HMP, HPMCs, LMP and CMC. In general, doughs  with modified celluloses and NaCl showed a decrease of a-helix conformation. CMC dough showed the  smallest percentage of a-helix conformation, and the highest contributions of more unfolded structures.  Doughs with pectins and NaCl showed similar percentages of a-helix to control one but an increase of  random coil structure was observed. Electrophoresis assays confirmed that the presence of certain hydrocolloids  (CMC) during gluten formation could affect protein interaction promoting subunits lability  from the matrix.","palabras_clave":"Wheat dough|Modified celluloses|Pectins|Gluten network"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64572,"idioma_id":4,"titulo":"Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications","resumen":"Current problems of filamentous  fungi fermentations and their further successful developments as microbial cell  factories are dependent on control fungal morphology. In this connection, this  work explored new experimental procedures in order to quantitatively check the  potential of some culture conditions to induce a determined fungal morphology  by altering both hyphal morphology and conidia adhesion capacity. The capacity  of environmental conditions to modify hyphal morphology was evaluated by  examining the influence of some culture conditions on the cell wall lytic  potential of Aspergillus niger MYA 135. The relative value of the cell  wall lytic potential was determined by measuring a cell wall lytic enzyme  activity such as the mycelium-bound â-N-acetyl-D-glucosaminidase (Mb-NAGase).  On the other hand, the quantitative value of conidia adhesion was considered as  an index of its aggregation capacity. Concerning microscopic morphology, a  highly negative correlation between the hyphal growth unit length (lHGU)  and the specific Mb-NAGase activity was found (r=&#8722;0.915, P&lt;0.001). In fact,  the environment was able to induce highly branched mycelia only under those culture  conditions compatible with specific Mb-NAGase values equal to or higher than  190 U gdry.wt-1. Concerning macroscopic  morphology, a low conidia adhesion capacity was followed by a dispersed  mycelial growth. In fact, this study showed that conidia adhesion units per ml  equal to or higher than 0.50 was necessary to afford pellets formation. In  addition, it was also observed that once the pellet was formed the lHGU  had an important influence on its final diameter. Finally, the biotechnological  significance of such results was discussed as well.","palabras_clave":"Aspergillus niger|Culture conditions|â-N-Acetyl-D-Glucosaminidase|Hyphal morphology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64573,"idioma_id":5,"titulo":"Los aniversarios del 24 de marzo en Clarín: de la celebración al amargo recuerdo","resumen":"Los medios de comunicación masiva ocupan un lugar destacado en nuestras sociedades. Como productores y difusores de determinadas representaciones acerca del presente y del pasado, intervienen en el espacio público, en tanto actores centrales en las dinámicas de poder. En los conflictos entre interpretaciones del pasado que emergen de los procesos de construcción social de la memoria colectiva, consideramos que los medios de comunicación, lejos de ser un mero registro neutral de memorias en pugna, constituyen productores de primer orden que participan en la elaboración, legitimación y puesta en circulación de algunos relatos sobre otros.  Partiendo de dicha premisa, el presente trabajo se propone revisar algunos aspectos de la relación entre medios de comunicación y memoria, a partir del análisis del tratamiento otorgado por el diario Clarín en sus editoriales a los aniversarios del golpe de Estado del 24 de marzo de 1976, y de los cambios en el modo en que el diario ?conmemoró? esa fecha entre 1977 y 1983. Se considera que la acción narradora de este medio de prensa le permitió elaborar interpretaciones del acontecimiento que se modificaron en función de la coyuntura en la que se insertaba el aniversario, las relaciones entre el matutino y las autoridades castrenses, y la necesidad de mantener el contrato de lectura con sus lectores.","palabras_clave":"Clarín|dictadura|conmemoraciones|24 de marzo"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64574,"idioma_id":4,"titulo":"Pretreatment of an induced mycelium-bound lipase from Arpergillus niger MYA 135 improves its hydrolytic and synthetic activity","resumen":"Whole-cell enzymes have been used as biocatalysts in a variety of reactions, such as free fatty acid production and the synthesis of fatty acid esters. In the present study, enzyme pretreatments with PEG, MES, Tween 80, Saponine, MgCl2.H2O, CaCl2 and different pH values were evaluated by using the Plackett?Burman statistical design to improve both the hydrolytic and synthetic activity of an induced mycelium-bound lipase from Aspergillus niger MYA 135. Interestingly, the preincubation at pH 4 had a significant effect on both the hydrolytic and transesterification activity, demonstrating the influence of the correct ionisation state on these activities. Meanwhile, the enzyme pretreatment with MgCl2 for in situ water activity control positively affected the esterification catalyst. Thus, compared with the control without pretreatment, the hydrolytic and the transesterification activities increased to 60.1 and 60.8 %, respectively, and with respect to the esterification reaction, the conversion was improved 2.33 times. Based on these results, by applying a simple pretreatment to the biocatalyst, the catalyst?s activity toward hydrolysis and synthesis was enhanced.","palabras_clave":"PLACKETT-BURMAN|MYCELIUM-BOUND LIPASE|HYDROLYSIS|SYNTHESIS|ASPERGILLUS NIGER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64575,"idioma_id":4,"titulo":"Xanthan chain length is modulated by increasing the availability of the polysaccharide copolymerase protein GumC and the outer membrane polysaccharide export protein GumB","resumen":"Xanthan is a polysaccharide secreted by Xanthomonas campestris that contains pentamericrepeat units. The biosynthesis of xanthan involves an operon composed of 12 genes (gumB to gumM). In this study, we analyzed the proteins encoded by gumB and gumC. Membranefractionation showed that GumB was mainly associated with the outer membrane, whereasGumC was an inner membrane protein. By specific globomycin inhibition, GumB was characterized as a lipoprotein. By reporter enzyme assays, GumC was shown to contain twotransmembrane segments flanking a large periplasmic domain. We confirmed that gumB and gumC mutant strains uncoupled the synthesis of the lipid-linked repeat unit from thepolymerization process. We studied the effects of gumB and gumC gene amplification on the production, composition, and viscosity of xanthan. Overexpression of GumB, GumC, or GumB and GumC simultaneously did not affect the total amount or the chemical composition of the polymer. GumB overexpression did not affect xanthan viscosity; however, a moderate increase in xanthan viscosity was achieved when GumC protein levels were increased five-fold. Partial degradation of GumC was observed when only that protein was overexpressed; but coexpression of GumB and GumC diminished GumC degradation and resulted in higher xanthan viscosity than individual GumB or GumC overexpression. Compared with xanthan from the wildtype strain, longer polymer chains from the strain that simultaneously overexpressed GumB and GumC were observed by atomic force microscopy. Our results suggest that GumB-GumC protein levels modulate xanthan chain length, which results in altered polymer viscosity.","palabras_clave":"membrane protein|polysaccharide chain length|xanthan|Xanthomonas campestris"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64576,"idioma_id":5,"titulo":"La secularización de la asistencia al moribundo: del cuidado cristiano en hospices a la expertise técnica de la medicina paliativa","resumen":"El objetivo del artículo es analizar el proceso de secularización de la atención a los moribundos, tomando como eje el devenir del movimiento de los hospices y los cuidados paliativos.&nbsp; El artículo repasa el surgimiento del movimiento de los hospices, de marcada inspiración religiosa, y el posterior desarrollo de la medicina paliativa como disciplina médica especializada en la atención de pacientes en el final de la vida.&nbsp; A partir de una revisión de fuentes bibliográficas y de investigaciones en curso, el artículo analiza el proceso de secularización de este movimiento, sosteniendo que este proceso no remite meramente a un cambio de contextos y de especialistas en la atención de los pacientes moribundos (del hospice al hospital, de los voluntarios al equipo profesional multidisciplinario) sino a la reapropiación de las esferas y temas propuestos en la filosofía del cuidado de los hospices &nbsp;por parte de un dispositivo médico y científico. &nbsp;","palabras_clave":"secularización|fin de vida|cuidados paliativos|historia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64577,"idioma_id":4,"titulo":"Validation of a Stability indicating RP-HPLC Method for the Determination of Gatifloxacin in eye drops","resumen":"A reversed-phase liquid chromatography (RP-LC) method was validated for the determination of gatifloxacin in eye drops. The LC method was carried out on a Phenomenex LiChrosphere 5 µm RP-18 100 &#506; 125 x 4.6 mm maintained at room temperature. The mobile phase consisted of water:acetonitrile:triethylamine (80:20:3 v\/v\/v), pH adjusted to 3.3 using phosphoric acid, run at a flow rate of 1 mL\/min and using ultraviolet detection at 293 nm. The chromatographic separation was obtained with a retention time of 3.6 min and was linear in the range of 30-70 µg\/mL (r2 = 0.9991). The specificity and stability indicating the capability of the method was proven through forced degradation studies, which also showed that there was no interference of the excipients. The accuracy was 100.63% with RSD = 1.65. Method validation demonstrates satisfactory results for precision and robustness. The proposed method was applied for the analysis of marketed eye drops, for improving the quality control and to assure the therapeutic efficacy.","palabras_clave":"Gatifloxacin|assay|HPLC|stability-indicating method"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64578,"idioma_id":4,"titulo":"Improving accessibility of Web interfaces: refactoring to the rescue","resumen":"Universal access should be a target for all public Web sites. However, it is very hard to achieve, and even Web applications that comply with accessibility standards may still lack usability for disabled users. This paper proposes refactoring as an essencial technique to incrementally improve the accessibility and usability of a Web interface. Some accessibility refactorings are described and classified by the problems that each refactoring addresses. The way mainstream Web sites struggle with accessibility is illustrated, and two evaluations of email clients are presented as empirical evidence of the significance of accessibility refactorings at a low implementation cost.","palabras_clave":"Accessibility|Web Applications|Refactoring|Reengineering"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64579,"idioma_id":4,"titulo":"Development of typing methods based on pyrosequencing technology for the analysis of six bovine genes related to marbling","resumen":"Several methods (PCR-RFLP, OLA, DNA sequencing, PCR SSCP and ARMS-PCR) have been developed to detect allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and at the transition in the thyroglobulin 5´ leader sequence in two bovine breeds. All these mutations were associated with marbling. Most of the methodologies mentioned above are manual processes and therefore increases the time spent on the assay and limit the number of analyzed animals. We describe the development of pyrosequencing-based methods applicable to the mentioned mutations. These methods were validated analyzing control DNA samples previously typed by PCR-SSCP, PCR-RFLP and DNA sequencing belonging to Aberdeen Angus and Hereford breeds. These results demonstrated that this sequence-based method would be useful in high throughput genotyping SNPs of candidate genes in bovine breeds, making this method interesting as a rapid screening method for association or population studies.","palabras_clave":"BOVINE MOLECULAR MARKERS|POLYMORPHISM|PYROSEQUENCING|MARBLING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64580,"idioma_id":4,"titulo":"First Comparative Transcriptomic Analysis of Wild Adult Male and Female Lutzomyia longipalpis, Vector of Visceral Leishmaniasis","resumen":"Leishmaniasis is a vector-borne disease with a complex epidemiology and ecology. Visceral leishmaniasis (VL) is its most severe clinical form as it results in death if not treated. In Latin America VL is caused by the protist parasite Leishmania infantum (syn. chagasi) and transmitted by Lutzomyia longipalpis. This phlebotomine sand fly is only found in the New World, from Mexico to Argentina. However, due to deforestation, migration and urbanisation, among others, VL in Latin America is undergoing an evident geographic expansion as well as dramatic changes in its transmission patterns. In this context, the first VL outbreak was recently reported in Argentina, which has already caused 7 deaths and 83 reported cases.    Insect vector transcriptomic analyses enable the identification of molecules involved in the insect´s biology and vector-parasite interaction. Previous studies on laboratory reared Lu. longipalpis have provided a descriptive repertoire of gene expression in the whole insect, midgut, salivary gland and male reproductive organs. Nevertheless, the study of wild specimens would contribute a unique insight into the development of novel bioinsecticides. Given the recent VL outbreak in Argentina and the compelling need to develop appropriate control strategies, this study focused on wild male and female Lu. longipalpis from an Argentine endemic (Posadas, Misiones) and a Brazilian non-endemic (Lapinha Cave, Minas Gerais) VL location. In this study, total RNA was extracted from the sand flies, submitted to sequence independent amplification and high-throughput pyrosequencing. This is the first time an unbiased and comprehensive transcriptomic approach has been used to analyse an infectious disease vector in its natural environment. Transcripts identified in the sand flies showed characteristic profiles which correlated with the environment of origin and with taxa previously identified in these same specimens. Among these, various genes represented putative targets for vector control via RNA interference (RNAi).","palabras_clave":"Transcriptomics|Leishmaniasis|Lutzomyia longipalpis|Bioinformatics"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64581,"idioma_id":4,"titulo":"ALLATOTROPIN: AN ANCESTRAL MYOTROPIC NEUROPEPTIDE INVOLVED IN FEEDING","resumen":"Background: Cell-cell interactions are a basic principle for the organization of tissues and organs allowing them to perform integrated functions and to organize themselves spatially and temporally. Peptidic molecules secreted by neurons and epithelial cells play fundamental roles in cell-cell interactions, acting as local neuromodulators,neurohormones, as well as endocrine and paracrine messengers. Allatotropin (AT) is a neuropeptide originally described as a regulator of Juvenile Hormone synthesis, which plays multiple neural, endocrine and myoactive rolesin insects and other organisms.Methods: A combination of immunohistochemistry using AT-antibodies and AT-Qdot nanocrystal conjugates wasused to identify immunoreactive nerve cells containing the peptide and epithelial-muscular cells targeted by AT inHydra plagiodesmica. Physiological assays using AT and AT- antibodies revealed that while AT stimulated theextrusion of the hypostome in a dose-response fashion in starved hydroids, the activity of hypostome in hydroids challenged with food was blocked by treatments with different doses of AT-antibodies.Conclusions: AT antibodies immunolabeled nerve cells in the stalk, pedal disc, tentacles and hypostome. AT-Qdot conjugates recognized epithelial-muscular cell in the same tissues, suggesting the existence of anatomical and functional relationships between these two cell populations. Physiological assays indicated that the AT-like peptide is facilitating food ingestion.Significance: Immunochemical, physiological and bioinformatics evidence advocates that AT is an ancestral neuropeptide involved in myoregulatory activities associated with meal ingestion and digestion.","palabras_clave":"HORMONE|ALLATOTROPINE|HYDRA|Q-DOTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64582,"idioma_id":4,"titulo":"Noise Minimisation in Gene Expression Switches","resumen":"Gene expression is subject to stochastic variation which leads to fluctuations in the rate of protein production. Recently, a study in yeast at a genomic scale showed that, in some cases, gene expression variability alters phenotypes while, in other cases, these remain unchanged despite fluctuations in the expression of other genes. These studies suggested that noise in gene expression is a physiologically relevant trait and, to prevent harmful stochastic variation in the expression levels of some genes, it can be subject to minimisation. However, the mechanisms for noise minimisation are still unclear. In the present work, we analysed how noise expression depends on the architecture of the cis-regulatory system, in particular on the number of regulatory binding sites. Using analytical calculations and stochastic simulations, we found that the fluctuation level in noise expression decreased with the number of regulatory sites when regulatory transcription factors interacted with only one other bound transcription factor. In contrast, we observed that there was an optimal number of binding sites when transcription factors interacted with many bound transcription factors. This finding suggested a new mechanism for preventing large fluctuations in the expression of genes which are sensitive to the concentration of regulators.","palabras_clave":"GENE EXPRESSION|FLUCTUATIONS|COOPERATIVITY|MASTER EQUATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64583,"idioma_id":5,"titulo":"Comparación de técnicas para el aislamiento y recuento de levaduras y hongos dimórficos del filoplano de Nothofagus pumilio","resumen":"El filoplano es un importante hábitat de microorganismos. Este trabajo tuvo como objetivo probar distintos métodos para el aislamiento de levaduras y hongos dimórficos del filoplano de Nothofagus pumilio, la especie arbórea dominante de Patagonia. Los métodos probados fueron la impresión sobre medio sólido, agitación vertical con agua durante distintos tiempos, con la adición de surfactante (Tween 80) o abrasivos (arena); macerado y ultrasonido. Se estableció un método que combina la agitación vertical con la aplicación de ultrasonido como metodología apropiada para el aislamiento y recuento. Con este método se determinó que la densidad de levaduras y hongos dimórficos en el filoplano de N.pumilio osciló entre las 10 y 103 unidades formadoras de colonias sobre cm2, valores entre 10 y 100 veces inferiores a los observados para otras especies arbóreas. También se estableció al hongo dimórfico Aureobasidium pullulans como la especie dominante de este ambiente.","palabras_clave":"Aureobasidium|Alta montaña|Lenga|Levaduras|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64584,"idioma_id":5,"titulo":"Hacia una gestión cultural de los espacios comunes","resumen":"La política cultural de los espacios públicos de la ciudad que presentamos a continuación no puede ser escindida del conjunto de intervenciones sobre la reproducción ampliada de la vida implicado en la política social, en tanto no solo busca intervenir en el entramado de relaciones socio-culturales de la ciudad, sino remover ciertas dinámicas y mecanismos profundos de la desigualdad social. Pensamos la política cultural de los espacios comunes como una intervención que, articulada con adecuadas políticas sociales y urbanas,  constituye un instrumento para modificar la configuración cultural de la ciudad: los usos y las apropiaciones de los espacios públicos, las posibilidades de desplazamientos y de encuentros entre distintos actores sociales, las formas predominantes de interacción social cotidiana en la ciudad.","palabras_clave":"GESTION CULTURAL|ESPACIOS COMUNES|LUGARES|MOVILIDADES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64585,"idioma_id":4,"titulo":"Mechanisms of DNA damage by photoexcited 9-methyl-b-carbolines","resumen":"It is known that â-carbolines are able to produce  photosensitized damage in cell-free DNA, but there is little information on  their effects on cellular DNA. Therefore, we have analyzed the DNA damage produced  by harmine and 9-methyl-harmine under UVA irradiation in V79 cells, together  with the associated generation of micronuclei and photocytotoxicity. The  results indicate that the most frequent photoproducts generated in the cellular  DNA are oxidized purines such as 8-oxo-7,8-dihydroguanine. Only relatively few  single-strand breaks were observed. Cyclobutane pyrimidine dimers were absent,  although they were generated in cell-free DNA irradiated under the same  conditions. The overall extent of DNA damage in the cells was considerably  smaller than the one observed in cell free DNA. The generation of cellular DNA  damage was associated with a significant generation of micronuclei and  decreased cell proliferation. The data indicate that â-carbolines act as  photosensitizers in mammalian cells. The spectrum of DNA modification, and  therefore the mechanism of DNA damage generation, differs considerably from  that observed with cell-free DNA.","palabras_clave":"Photochemistry|Carbolines|9-Me-carbolines|DNA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64586,"idioma_id":4,"titulo":"Assessment of long-range correlation in animal behavior time series: the temporal pattern of locomotor activity of Japanese quail (Coturnix coturnix) and mosquito larva (Culex quinquefasciatus)","resumen":"The aim of this study was to evaluate the performance of a classical method of fractal analysis, Detrended Fluctuation Analysis (DFA), in the analysis of the dynamics of animal behavior time series. In order to correctly use DFA to assess the presence of long-range correlation, previous authors using statistical model systems have stated that different aspects should be taken into account such as: 1) the establishment by hypothesis testing of the absence of short term correlation, 2) an accurate estimation of a straight line in the log-log plot of the fluctuation function, 3) the elimination of artificial crossovers in the fluctuation function, and 4) the length of the time series. Taking into consideration these factors, herein we evaluated the presence of long-range correlation in the temporal pattern of locomotor activity of Japanese quail (Coturnix coturnix) and mosquito larva (Culex quinquefasciatus). In our study, modeling the data with the general ARFIMA model, we rejected the hypothesis of short range correlations (d=0) in all cases. We also observed that DFA was able to distinguish between the artificial crossover observed in the temporal pattern of locomotion of Japanese quail, and the crossovers in the correlation behavior observed in mosquito larvae locomotion. Although the test duration can slightly influence the parameter estimation, no qualitative differences were observed between different test durations","palabras_clave":"Detrended Fluctuation Analysis|Fractal|Animal behavior|Crossovers"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64587,"idioma_id":4,"titulo":"Geotourism attractions in intermediate destinations of peripheral regions","resumen":"This study discusses the development of palaeontologic geosites, with and without fossils remains in situ, as spaces of leisure in peripheral regions. A factor analysis was carried out on 115 geosites at   natural surroundings, in eleven palaeontologic areas of North Patagonia (Provinces of Neuquen and Rio Negro, Argentina). The major research findings were four factors. 1) The exterior linkage with other   attractions that makes a reference to a space of flows, 2) The distance to a populated centre that is related with the emerge of specialized segments of demand, 3) The management capacity where the role   of the local community is fundamental, 4) The state-support for the setting in value of natural heritage attractions without being based on a profitability criteria. These factors contributed to understand the   location of a new tourist product and the role of the secondary attractions in the incorporation of peripheral regions to the territory of tourism","palabras_clave":"Paleontología|Clasificación multidimensional"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64588,"idioma_id":5,"titulo":"NUEVA METODOLOGÍA PARA EL CÁLCULO DEL EFECTO TOPOGRÁFICO PARA LA CORRECCIÓN DE DATOS SATELITALES","resumen":"Presentamos el desarrollo de un programa denominado DEM2tess, que permite convertir un modelo digital de elevaciones en un conjunto de prismas esféricos denominados teseroides. Estos últimos se utilizan como datos de entrada en el programa Tesseroids-V2, para la obtención del potencial anómalo y las componentes del tensor del gradiente gravimétrico. Esta nueva metodología permite realizar el cálculo del efecto topográfico que será aplicado a datos gravimétricos y gradiométricos satelitales. El programa Tesseroids (Uieda et al. 2010), fue modificado respecto de su versión original la cual solo permitía el cálculo con modelos sintéticos. El uso del programa DEM2tess en conjunto con el Tesseroid-V2, permiten realizar cálculos utilizando la topografía real, transformando los datos en un sistema de coordenadas geocéntricos, y de ésta manera comparar los resultados directamente con los datos satelitales. Esta nueva propuesta mejora hasta 50 veces el tiempo de proceso respecto del programa Tesseroids, lo cual es fundamental para el cálculo con grillas de datos topográficos a escala regional. ","palabras_clave":"Efecto topográfico|Modelos globales de gravedad|GOCE|Teseroides|Modelo digital de elevaciones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64589,"idioma_id":5,"titulo":"Calidad ambiental y nivel socioeconómico: Su articulación en la Región Metropolitana de Buenos Aires","resumen":"En los estudios de Calidad de vida, la Calidad ambiental y el Nivel socioeconómico constituyen dimensiones del bienestar de la población y aparecen integrados en índices complejos. Sin embargo, cabe preguntarse en qué medida y de qué modo se articulan ambas dimensiones en medios urbanos, donde los patrones de asentamiento de la población responden a un complejo entramado pluricausal. En este artículo se intenta dar respuesta a este interrogante a partir del caso de la Región Metropolitana de Buenos Aires. La Calidad ambiental es estudiada mediante un índice especialmente diseñado a tal fin y el nivel socioeconómico a través de un indicador indirecto (el clima educativo de los hogares). El análisis se divide en dos partes: la primera refiere al estudio de las dimensiones en las jurisdicciones que componen la Región Metropolitana de Buenos Aires, mientras que la segunda indaga la relación espacial entre ellas.","palabras_clave":"Calidad ambiental|Nivel socioeconómico|RMBA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64590,"idioma_id":5,"titulo":"Fragmentacion socioterritorial y condiciones de vida en la Argentina en los albores del siglo XXI.","resumen":"El presente estudio aborda la problemática de la fragmentación social en la Argentina. La noción de fragmentación asocia componentes espaciales ydimensiones económico-sociales y políticas. Esta problemática se expresa en el marco territorial,entendiendo como tal la relación entre naturalezay sociedad a lo largo del tiempo. En este contexto, se propone el uso de una herramienta que permita detectar la distribución espacial de las Condiciones de vida como una aproximación al conocimiento de dicha fragmentación socioterritorial. Al mismo tiempo, a través del concepto de brecha, nos aproximamos también a su proceso histórico.La fragmentación se expresa en lo que llamamos, al menos provisoriamente, las dos Argentinas, definidas sobre la base del Índice Sintético de Condiciones de Vida (iscv) aplicado en el año 2001. Estos territorios albergarían en su interior distintas sociedades y diferentes problemas que requerirían un marco de análisis concreto que permita generar soluciones específicas.","palabras_clave":"FRAGMENTACION SOCIOTERRITORIAL|CONDICIONES DE VIDA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64591,"idioma_id":5,"titulo":"ESTUDIOS SOBRE GEOGRAFÍA Y CALIDAD DE VIDA EN LA ARGENTINA.  CINCO DÉCADAS DE APORTES BIBLIOGRÁFICOS","resumen":"El estudio de la calidad de vida puede ser abordado desde diferentes disciplinas,produciendo gran variedad de contenidos. El enfoque geográfico parte de una base espacialy territorial que le permite establecer diferencias con respecto a otras perspectivasinvolucradas en el estudio de la calidad de vida. La capacidad que posee para realizaranálisis sobre la configuración espacial de diferentes variables le permite tener unaaproximación mayoritariamente empírica. Una de las características por las que se destacala ciencia geográfica es la elaboración de cartografía que permite llevar el análisis alterritorio, recurriendo a diferentes escalas que consienten desde el estudio a escala nacional hasta otras más analíticas en las que se puede observar el espacio cotidiano en el que sedesenvuelven las personas. El trabajo que presentamos propone un recorrido bibliográficodesde los inicios hasta la actualidad del fructífero vínculo existente entre el estudio de lacalidad de vida y la Geografía. Esperamos que las referencias mencionadas permitanconocer mejor los aportes que realiza nuestra ciencia y, a la vez, sirvan para enriquecer losanálisis que efectuados por otras disciplinas.hasta otras más analíticas en las que se puede observar el espacio cotidiano en el que sedesenvuelven las personas. El trabajo que presentamos propone un recorrido bibliográficodesde los inicios hasta la actualidad del fructífero vínculo existente entre el estudio de lacalidad de vida y la Geografía. Esperamos que las referencias mencionadas permitanconocer mejor los aportes que realiza nuestra ciencia y, a la vez, sirvan para enriquecer losanálisis que efectuados por otras disciplinas.","palabras_clave":"Geografía|Calidad de Vida|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64592,"idioma_id":4,"titulo":"Crosstalk among dietary polyunsaturated fatty acids, urolithiasis, chronic inflammation, and urinary tract tumor risk.","resumen":"Abstract: Based on a consistent bulk of experimental and epidemiological works, we proposed thatabnormal metabolism and\/or dietary deprivation of essential PUFAs by inducing a chronic andsubclinical EFA deficiency in urothelial cell membranes may enhance the risk for urinary tract tumor(UTT) development. This threat may be enhanced by the unusual fact that the fatty acid profile of thenormal urothelium is similar to that reported in EFA deficiency. The risk for UTT may be worsenedwhen co-existing with a low grade chronic inflammation (LGCI) state induced by urolithiasis ordisbalance management of peroxides, free radicals molecules and their quenchers. There is cumulativeevidence linking the LGCI of the UT mucosa, calculi and UTT, due to the long-standing release ofpromitotic, promutagen and pro-inflammatory antiapoptotic cytokines in these conditions. The dualrole played by pro- and anti-inflammatory eicosanoids and bioactive lipids (BALs), cytokines and thedisbalance of lipid peroxidation is discussed, concluding that the moderate, long-standingconsumption or dietary supplementation of w3 PUFAs may improve the chances of avoiding UTTdevelopment.","palabras_clave":"urinary tract tumor|Bladder cancer|dietary PUFAs|inflammation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64593,"idioma_id":4,"titulo":"The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells","resumen":"The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH) glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on steroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.","palabras_clave":"FSH|Glycosylation variant|Steroid|Gene expression"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64594,"idioma_id":4,"titulo":"RDAVIDWebService: a versatile R interface to DAVID","resumen":"Summary: the RDAVIDWebService package provides a class-based interface from R programs\/scripts to fully access\/control the Database for Annotation, Visualization and Integrated Discovery (DAVID), without the need for human interaction on its website (http:\/\/david.abcc.ncifcrf.gov). The library enhances DAVID capabili-ties for Gene Ontology analysis by means of GOstats-based direct acyclic graph conversion methods, in addition to the usual many-genes-to-many-terms visualization.  Availability: RDAVIDWebService is available as an R package from the Bioconductor project (www.bioconductor.org) and on the au-thors? website (www.bdmg.com.ar) under GPL-2 license, subjected to the terms of use of DAVID  (http:\/\/david.abcc.ncifcrf.gov\/content.jsp?file=WS.html).  Contact: cfresno@bdmg.com.ar or efernandez@bdmg.com.ar","palabras_clave":"Gene expression|Functional genomics|Gene Ontology|Visualization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64595,"idioma_id":4,"titulo":"Molecular pathology of acute kidney injury in a choline- deficient model and fish oil protective effect","resumen":"Purpose  The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model.  Methods  Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed.  Results  All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats.  Conclusions  This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.","palabras_clave":"Acute kidney injury | Choline-deficient|Gene expression | Menhaden oil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64596,"idioma_id":4,"titulo":"Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L.","resumen":"Four somaclonal mutants (S1, S4, S6 and M10) and their parental Cenchrus ciliaris L. cultivar Biloela were characterized under salinity conditions at germination and vegetative growth stages. Seeds of all somaclonal mutants had higher germination percentages than cv. Biloela seeds in the control and salt treatments. At 150 mM, germination was significantly higher in M10, S6 and S4 (72.3%, 66.3% and 61.8%, respectively) than in cv. Biloela (35.5%). Mutants grown under salinity along with cv. Biloela for 35 days had a different relative growth rate. S6 had the highest growth rate, indicating its potential tolerance to salt stress, whereas M10 was the most sensitive, with Bi, S4 and S1 being intermediate tolerant genotypes. Catalase enzyme activity (CAT) in M10 decreased in response to salt stress and was significantly associated with malondialdehide content, suggesting salt injury, whereas higher levels of CAT activity in S6 during salt stress were associated with increased salinity tolerance. The present results indicate that somaclonal variation and in vitro mutagenesis offer an effective tool for improvement of C. ciliaris because the somaclonal mutants showed differential tolerance to salt stress with respect to their parental and could be a better choice for use in a breeding program.","palabras_clave":"SOMACLONAL MUTANTS|GERMINATION|VEGETATIVE GROWTH|SALT STRESS|CENCHRUS CILIARIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64597,"idioma_id":5,"titulo":"Políticas para evaluar la calidad de la educación en Argentina después de los 90","resumen":"En este trabajo presentamos una aproximación a las políticas de evaluación de la calidad en el nivel secundario en el contexto de la reforma educativa de los `90 y a partir de la legislación posterior, sancionada en los últimos años. El artículo  surge de los primeros avances en el desarrollo del Programa de Investigación \u201CLa evaluación de la calidad como política para el mejoramiento de la enseñanza secundaria\u201D  cuyo principal objetivo es caracterizar las políticas de evaluación de la calidad adoptadas a partir de la Ley Federal de Educación (1993) y la Ley de Educación Nacional (2006) para el último año del nivel secundario. Esta decisión se fundamenta en los problemas de cobertura y retención reconocidos por las instancias gubernamentales, así como en la evaluación de las medidas adoptadas a partir del establecimiento de la obligatoriedad del nivel secundario con la sanción de la Ley de Educación Nacional.","palabras_clave":"ESCUELA SECUNDARIA|CALIDAD DE LA EDUCACION|NORMATIVA EDUCACIONAL|EDUCACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64598,"idioma_id":4,"titulo":"Visible light polymerization of epoxy monomers using an iodonium salt with camphorquinone\/ethyl-4-dimethyl aminobenzoate","resumen":"A visible light initiator system for the photoinduced cationic polymerizationof epoxy monomers is reported.The system consists of camphorquinone (CQ) in combination with ethyl-4-dimethyl aminobenzoate (EDMAB) and a diaryliodonium salt (Ph2ISbF6). The three-component system efficiently photoinitiates the polymerization of monomers containing an epoxycyclohexane group, 3,4-epoxycyclohexylmethyl 3',4'-epoxycyclohexane carboxylate (UVR) and 1,3-bis(3,4-epoxycyclohexyl-2-ethyl),1,1,3,3-tetramethyldisiloxane (SIB), under irradiation with blue light (467 nm). Very rapid photopolymerization resulted from irradiation of SIB containing Ph2ISbF6 in combination with CQ and better results were obtained in the presence of EDMAB. On the other hand, no polymerization was detected after irradiation of UVR photoactivated with Ph2ISbF6 and CQ. However, this monomer polymerized readily and to high conversion when EDMAB was present. Moreover, almost complete conversion of UVR occurs in the absence of external heating. The polymer resulting from UVR displayed higher values of compressive and flexural properties than the polymer prepared from SIB. This is explained in terms of a higher density of crosslinking points in UVR which is accompanied by a lower content of non-reacted monomer; this has a plasticizing effect on the hardened material.","palabras_clave":"CATIONIC POLYMERIZATION|VISIBLE LIGHT|EPOXY|CAMPHORQUINONE|IODONIUM SALT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64599,"idioma_id":4,"titulo":"Encapsulants for light-emitting diodes from visible light-cured epoxy monomers","resumen":"During the last decade, light-emitting diodes (LEDs) have replaced incandescent, fluorescent, and neon lamps due to their ability to produce high luminosity at low currents and voltages. LEDs are currently encapsulated by thermally curable epoxy resins. However, long periods of curing at high temperature result in high consumption of energy and require stringent process control to avoid failure of the devices. In addition, the thermal cure results in yellowing of the encapsulant, which decreases the efficiency of the LED. In recent years, photoinitiated polymerization has received much interest as it congregates a wide range of economic and ecological benefits. Cationic photoinitiators, such as diaryliodonium salts, generate Brønsted acid in situ, which initiates polymerization. The process can be triggered on demand by irradiating the mixture with light. Results from the present research reveal that cycloaliphatic epoxy monomers, photoactivated with an iodonium salt and Camphorquinone, polymerize readily under visible light irradiation (470 nm) in the absence of external heating. The partial replacement of cycloaliphatic epoxy with aromatic diglycidyl ether of bisphenol-A (DGEBA) is an effective means of improving the refractive indexof the material and consequently the efficiency of the photoemission. Visible light polymerization of DGEBA pure proceeds at a slow rate; however, it is enhanced by the increase in temperature during the polymerization of the highly reactive cycloaliphatic monomer. From results obtained in the present research, it may be concluded that visible light polymerization of epoxy monomers is a promising route for the processing of LED encapsulants.","palabras_clave":"PHOTOPOLYMERIZATION|VISIBLE LIGHT|LIGHT EMITTING DIODES|EPOXY|CATIONIC POLYMERIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64600,"idioma_id":4,"titulo":"Hybrid organic-inorganic nanocomposites based on poly (hydroxyethyl methacrylate) \/ silica (PHEMA\/SiO2)","resumen":"Hybrid organic-inorganic nanocomposites were prepared through the simultaneous polymerization of 2-hydroxyethyl methacrylate and tetraethoxysilane (TEOS) by the sol-gel process. The resulting PHEMA\/SiO2 films (~ 1.5 mm thick) were rigid and optically transparent. The films were crushed into fine particles which were used as filler for light-cured dental composite resins based on methacrylate monomers. The presence of methacrylate groups in the PHEMA\/SiO2 particles improves their compatibility with the methacrylate monomer used as matrix of the composite resins. This facilitates the incorporation of high amount of filler into the resins. The composites containing particles based on PHEMA\/SiO2 offer the possibility of reduced polymerization shrinkage without severe reductions in flow characteristics of the prepolymerized resin. The amount of reinforcing filler in the final composite will be determined as a compromise between optimizing both the rheological properties and the volumetric shrinkage without jeopardizing the mechanical properties required by a particular application","palabras_clave":"Solgel| hybrid organic-inorganic|photopolymerization"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64601,"idioma_id":4,"titulo":"Monitoring of visible light photopolymerization of an epoxy\/ dimethacrylate hybrid system by Raman and near-infrared spectroscopies","resumen":"Hybrid systems formulated with epoxy and methacrylate monomers at mass fraction either 50:50 or 75:25 were studied. The individual monomer conversions during photopolymerization with visible light were monitored by Raman and near-infrared spectroscopies. The rate of polymerization and final degree of conversion of the methacrylate groups were raised when the epoxy monomer was present. This is attributed to enhancement of the mobility of the reactive species caused by the presence of the epoxy monomer. Conversely, the earlier vitrification of the system due to the faster polymerizing methacrylate network resulted in reduced conversion of epoxy rings.","palabras_clave":"PHOTOPOLYMERIZATION|HYBRID |METHACRYLATE|EPOXY|RAMAN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64602,"idioma_id":5,"titulo":"Dynamic Characterization and Equalization of a Power Line Communication Channel","resumen":"Power Line Communication (PLC) systems need signal processing algorithms in order to recover the transmitted signals from the high degrading medium. In the literature exist some techniques that work on the basis of a constant, known channel, but it was demonstrated that the PLC channel is time variant, therefore these techniques lose efficiency. This work presents a dynamic equalization algorithm computed in the time domain, where the channel characterization is done by a recursive least squares algorithm using the transmitted data as reference. The proposal was tested with simulations and was statistically validated using in time variant PLC channel models, obtaining a lower error rate than other methods.","palabras_clave":"Power Line Communication|Equalization|Recursive Least Squares|Binary Error Rate"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64603,"idioma_id":5,"titulo":"DISEÑO DE ESTÉREO ESTRUCTURAS BASADO EN SU COMPORTAMIENTO MECÁNICO","resumen":"Es  notable  el  incremento  que  se  ha  dado  en  la  construcción  de  cubiertas  realizadas  con           estéreo  estructuras  metálicas.  Por  ello,  resulta  importante  establecer  parámetros  de  diseño,con  lafinalidad de obtener estructuras más económicas y con una adecuada seguridad.          En este trabajo se presenta un análisis, realizado con una técnica de optimización, para el diseño de lageometría  de  estéreo  estructuras  deacero  de  base  rectangular.  Se  analiza  mecánicamente  estatipología estructural para diferentes condiciones geométricas y vinculación, a los efectos de reducir elpeso  total  de  la  misma  y  las  deformaciones  en  sus  nudos,  dos  parámetros  que  varían  en  forma antagónica.  La  metodología  empleada,  para  efectuar  este  proceso  de  optimización,  consiste  en minimizar   separadamente   dos   funciones   objetivo:   Desplazamientos   Volumétricos   y   TensionesVolumétricas.  El  Desplazamiento  Volumétrico  está  definido  como  la  sumatoriadel  producto  del           desplazamiento de cada nodo, por el volumen de influencia de las barras que concurren a dicho nudo.           Un procedimiento similar se aplica para la función objetivo denominada Tensiones Volumétricas. Para realizar  los  análisis  mecánicos  de  lasdistintas  estructuras,  se  recurre  al  empleo  del  Método  de  los Elementos Finitos.          Específicamente, la variable que se optimiza es la altura (también denominada, espesor)           medida entrelas capas superior e inferior de las estéreo estructuras y el ancho, en pl          anta, de los módulos.","palabras_clave":"ESTEREO ESTRUCTURAS|OPTIMIZACION ESTRUCTURAL|DESPLAZAMIENTOS VOLUMETRICOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64604,"idioma_id":5,"titulo":"Modelos de cálculo de las Betas a aplicar en el CAPM: El caso de Argentina","resumen":"                    Producto de una revisión  de la literatura, en el presente trabajo se aplican seis métodos para el  cálculo de las betas de una muestra de once compañías que entre 2010 y 2012  cotizaron en el Mercado de Valores de Argentina. Empleando cada método, se  identifica aquel que puede ser tomado como referencia para determinar las betas  de empresas PyMEs que no cotizan en la Bolsa de Valores. Se concluye que para  calcular los valores de las betas e interpretar el riesgo de cada empresa,  resulta necesario analizar técnicamente el método utilizado y la variabilidad  de las series temporales empleadas, además de conocer las perspectivas futuras  tanto de la empresa analizada como del sector al cual pertenece  ","palabras_clave":"BETA|RIESGO |CAPM|PYMES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64605,"idioma_id":5,"titulo":"Particularidades del Modelo de Fijación de Precios de Activos de Capital (CAPM) en Mercados Emergentes","resumen":"El trabajo analiza el estado del arte del desarrollo de los aspectos a tener en cuenta para aplicar el ModeloCAPM en mercardos emergentes, como medio para calcular la rentabilidad de activos de capital y a su vez elcosto de capital propio de los inversores, utilizado a su vez en el modelo de Costo Promedio Ponderado de Capital,para el cálculo de la tasa de descuento de flujos de fondos de alternativas de inversión. Se presentan propuestastanto de ajustes de variables propias del Modelo CAPM (Tasa libre de riesgo y beta), como otras alternativas queincorporan al modelo tradicional primas como las de país y mercado, estableciendo así un marco de referenciapara nuevos desarrollos que permitan disminuir la brecha existente en el nivel de aplicación del Modelo CAPMpara inversores en mercados eficientes y aquellas analizadas para mercados emergentes.","palabras_clave":"CAPM|MERCADOS EMERGENTES|TASA LIBRE DE RIESGO|RIESGO PAÍS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64606,"idioma_id":5,"titulo":"EVALUACIÓN FINANCIERA DE PROYECTOS DE INVERSIÓN EL CASO DE ARGENTINA","resumen":"            La evaluación financiera de los  proyectos de inversión suele ser el instrumento principal para la decisión de  invertir. En este trabajo se analizan las condiciones necesarias para promover  esas decisiones a la luz de las experiencias locales e internacionales. La evaluación  financiera suele hacerse por medio de una o más herramientas: el cálculo de la  TIR, del VPN o del Período de Repago, analizamos las mismas y seleccionamos las  más relevantes para el contexto local.  ","palabras_clave":"TASA DE DESCUENTO|PAÍSES EMERGENTES|PERÍODO DE REPAGO|EVALUACIÓN DE PROYECTOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64607,"idioma_id":5,"titulo":"Crecimiento y supervivencia de plántulas de cinco especies de Acacia (Fabaceae) que coexisten en bosques secos neotropicales de Argentina, en distintas condiciones de disponibilidad de luz y agua","resumen":"El establecimiento de la plántula es una de las etapas más riesgosas para las plantas, especialmente en zonas áridas y semiáridas donde la sequía y alta radiación solar influyen sobre su emergencia, desarrollo y supervivencia. Se evaluó en invernadero la supervivencia y variables de crecimiento en plántulas sometidas a estrés hídrico y a distintas condiciones de luz, en cinco especies de Acacia (A. aroma, A. caven, A. atramentaria, A. gilliesii y A. praecox) que coexisten en los bosques xerófilos de Córdoba. Aunque se encontraron diferencias entre las especies (F = 5.66, p = 0.001), todas tuvieron altos porcentajes de supervivencia en las distintas condiciones de luz y agua, sugiriendo que serían tolerantes al estrés hídrico y podrían establecerse bajo luz o sombra. Si bien todas las especies mejoraron el crecimiento con luz y sin estrés hídrico, A. aroma, A. caven y A. atramentaria mostraron una tendencia hacia un mayor crecimiento en la mayoría de las variables consideradas (F = 41.9, p < 0.0001; F = 7.06, p < 0.0001; F = 53.59, p < 0.0001). Estos resultados sumados a otros ya reportados indicarían una diferenciación de nichos regenerativos favoreciendo la coexistencia regional de estas especies en los bosques xerófilos de Córdoba.","palabras_clave":"ACACIA|PLÁNTULA|SOMBRA|ESTRÉS HÍDRICO|COEXISTENCIA|BOSQUE SEMIÁRIDO|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64608,"idioma_id":5,"titulo":"La pobreza y el ritual de la reciprocidad y sacrificio popular de familias que viven en la localidad de Tinogasta","resumen":"El presente trabajo pretende reflejar las relaciones de reciprocidad y sacrificio popular que  teje y consolida  un vecindario en un contexto urbano para lograr subsistir y\/o superar una situación de pobreza o necesidad urgente. Se indaga sobre la existencia de lazos de reciprocidad incluidas en formas de trasmitir contención, amistad, igualdad social y económica, independencia jerárquica en las relaciones familiares y vecinales, las cuales se traducen en estrategias de subsistencia que permiten acceder a recursos para su reproducción cotidiana. Nuestra investigación pretende, relatar y analizar las relaciones de reciprocidad cotidianas, que han sido observadas de manera participativa y registradas en 4 familias de la localidad de Tinogasta-Catamarca. Nos surgen los siguientes interrogantes ¿Cuáles son los significados que los sujetos desde la materialidad corporal  y  simbólica le dan a las relaciones de reciprocidad y sacrificio popular para lograr su subsistencia cotidiana en una situación de pobreza? y ¿Cómo operan las relaciones familiares de reciprocidad y sacrificio popular en el  interior de un contexto urbano?Desde una perspectiva cuantitativa y cualitativa a través de un enfoque etnográfico se realizaron los registros de las acciones de los sujetos. Esto nos permitió detectar la multiplicidad y diversidad de las relaciones. Que permiten que los sujetos, se benefician por la institución aleatoria de redes de apoyo sociales más justas, ya que todos tienen la opción de dar y recibir","palabras_clave":"Familia - pobreza \u2013 reciprocidad - sacrificio popular."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64609,"idioma_id":5,"titulo":"Resumen, análisis y recomendaciones del reporte semestral VIHDA","resumen":"x","palabras_clave":"reporte semestral |VIHDA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64610,"idioma_id":10,"titulo":"La Tecnología de la Información en la Prevención y Control de Infecciones","resumen":"x","palabras_clave":"Tecnología de la Información |Prevención y Control de Infecciones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64611,"idioma_id":4,"titulo":"Human Papillomavirus (HPV) in oral cavity lesions: Comparison with other oral cancer risk factors","resumen":"Abstract Human papillomavirus (HPV) is considered a necessary factor for the development of cervical cancer;   however, its relationship with oral cancer is controversial. The aim of this study was detect the presence of HPV in lesions   of the oral cavity and its correlation with other risk factors. Presence of HPV was studied by polymerase chain reaction in   samples from benign lesions, potentially malignant lesions (PML), neoplasias and healthy mucosae. The results from the   different groups were compared; in addition to their histopathological variables with tobacco smoking, alcohol   consumption, so on. HPV was detected in 88.89% of benign lesions, 41.38% of PML and 56.25% of neoplasias. The most   prevalent genotypes were 16 and 6. Together, reached 55% of the total number of cases. A significant association was   observed between HPV and male gender, tobacco smokers, alcohol drinkers and benign lesions. Tobacco smoking and   alcohol intake were associated to neoplasias. Our results showed that factors like tobacco smoking and alcohol drinking,   have more influence than HPV in the development of oral neoplasias; however, 56.2% of the neoplasias tested positive for   HPV; the percentage of HR-HPV detection increased with the severity of the lesions, suggesting its possible involvement in   malignant processes","palabras_clave":"HPV|LESION|ORAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64612,"idioma_id":5,"titulo":"Modelo de Sistema Basado en Conocimiento en el Dominio de la Seguridad de Aplicaciones","resumen":"Resumen?El objetivo es proponer un modelo de un sistemabasado en conocimiento (SBC) aplicado al análisis de seguridadde aplicaciones de gestión. El modelo se fundamenta en unsistema basado en conocimiento (SBC) que cuenta con uncomponente cognitivo que le permite incorporar conocimiento.En virtud de que las amenazas y los ataques informáticosrepresentan un problema constante y creciente se puede suponerque el SBC, a través del aprendizaje dinámico que lo mantendráactualizado, podrá asistir a los especialistas en Seguridad de laInformación, en el área de competencia, a la elaboración deEspecificación de Requerimientos.","palabras_clave":"Seguridad Aplicaciones Informaticas|Sistemas Basados en Conocimiento|Ingenieria de Requerimientos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64613,"idioma_id":4,"titulo":"An Efficient Spectral Shaping Method for OFDM Systems using Correlated Interpolation of Symbols","resumen":"The generation of suitable orthogonalfrequency division multiplexing (OFDM) signals onthe grounds of fully digital signal processing is con-sidered. The main objective is to obtain a discrete-time signal with adequate allocation of power emis-sions in both, in-band portion (i.e., the allocatedband for communication) and out-of-band portion(i.e., the band allocated to adjacent channels) of thespectrum. The proposed method prevents the trans-mitter from using traditional filtering techniques, tokeep under control power emissions in the system. Inaddition, the adaptability feature of our proposalmakes its implementation attractive within cognitiveradio (CR) and software defined radio (SDR) OFDM-based systems. Our spectral shaping approach isbased on an optimum interpolation, obtained fromthe combination of an inverse fast Fourier transform(IFFT) and a spectral precoding operation, both ofthem transparent from the perspective of a conven-tional (legacy) OFDM receiver.","palabras_clave":"correlated interpolation|ofdm |spectral shaping|double length"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64614,"idioma_id":5,"titulo":"Distribución y caracterización del daño ocasionado por Pissodes castaneus en plantaciones de Pinus spp. de la región Andino Patagónica Argentina","resumen":"El primer registro de Pissodes castaneus (Col.: Curculionidae) en la región Andino Patagónica fue realizado en 2005 en la provinciade Neuquén. Este insecto ataca coníferas del género Pinus, generalmente debilitadas por factores bióticos o abióticos. En algunasplantaciones constituye el único problema sanitario, sin embargo, se desconoce su área de distribución y los niveles de daño en laregión. Los objetivos del trabajo fueron: 1) determinar la distribución actual de la especie en la región Andino Patagónica, 2) tipificary cuantificar el daño ocasionado por Pissodes castaneus en plantaciones de la provincia de Chubut, y 3) evaluar la asociación entreintensidad de ataque y características silvícolas y ambientales de las plantaciones en la provincia de Chubut. La especie fue registradaen las provincias de Neuquén, Río Negro y Chubut, siendo proporcionalmente mayor el número de plantaciones infestadas en las dosúltimas. Los porcentajes de infestación variaron entre 1,4 y 45,7 %. Pinus contorta var. murrayana resultó más afectado y, en ordende importancia, le siguieron Pinus radiata y Pinus ponderosa. La mayor mortalidad se registró en Pinus contorta var. murrayana.En plantaciones jóvenes de Pinus ponderosa fue registrado como el único agente de daño, ocasionando la muerte por anillamiento.El grado de infestación estuvo positivamente asociado con la presencia de residuos forestales y con el ataque de Sirex noctilio. Lascaracterísticas del ataque observado en la región, sumadas a la distribución que ha alcanzado la especie, exigen que Pissodes castaneussea considerado en la definición de pautas de manejo.","palabras_clave":"INFESTATION LEVEL|DISTRIBUTION|DAMAGES|SILVIVULTURAL MANAGEMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64615,"idioma_id":4,"titulo":"Crowdsourcing Mobile Web Applications","resumen":"Building Mobile Web or Hypermedia Applications is usually difficult   since there is a myriad of issues to take into account. Moreover adding   support for personalized or context-aware behaviors goes far beyond the   possibilities of many kinds of organizations that intend to build this   kind of software (museums, city halls, etc). In this article we present a   novel approach to delegate part of the effort in building mobile Web   software to developers outside those organizations or even to final   users. We show that this approach is feasible, light and practical and   present a set of experiments we developed to verify our claims.","palabras_clave":"Mobile Web Applications|Client-Side Adaptation|Crowdsoursing|Mobile Hypermedia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64616,"idioma_id":4,"titulo":"Hydrolysis of Lactose: estimation of kinetic parameters using Artificial Neural Networks","resumen":"      The analysis of any kinetic process  involves the development of a mathematical model with predictive purposes.  Generally, those models have characteristic parameters that should be estimated  experimentally. A typical example is Michaelis-Menten model for enzymatic  hydrolysis. Even though conventional kinetic models are very useful, they are  only valid under certain experimental conditions. Besides, frequently large  standard errors of estimated parameters are found due to the error of  experimental determinations and\/or insufficient number of assays. In this work  we developed an artificial neural network (ANN) to predict the performance of  enzyme reactors at various operational conditions. The net was trained with  experimental data obtained under different hydrolysis conditions of lactose solutions  or cheese whey, and different initial concentrations of enzymes or substrates.  In all the experiments commercial &#946;-galactosidase either free or immobilized in  a chitosan support was used. The neural network developed in this study had an  average absolute relative error of less than 5% even using few experimental  data, which suggests that this tool provides an accurate prediction method for  lactose hydrolysis.  ","palabras_clave":"CHESSE WHEY|BETA-GALACTOSIDASE|LACTOSE HYDROLYSIS|ARTIFICIAL NEURAL NETWORK"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64617,"idioma_id":5,"titulo":"Patrones espacio temporales de incendios en el Complejo Litoral del Río Paraná","resumen":"    Se ha estudiado la ocurrencia  de incendios en el Complejo Litoral del Río Paraná, período 2004-2008), mediante el análisis de focos de calor. Se evaluó información espacial de diferentes sensores remotos (MODIS, ERS, Goes, NOAA) descargados de diferentes fuentes de consulta (CONAE, ESA, INPE). Los datos del sensor MODIS-AQUA (descargados y procesados por&nbsp; CONAE), presentaron la mayor eficiencia en la detección de focos de calor. Para este sensor, se desarrolló un algoritmo que permitió agrupar focos de calor e interpretarlos como incendios, integrando conocimiento previo de la región y del comportamiento del fuego en el área de estudio. Como resultados se obtuvieron mapas, tablas y figuras. Se realizaron estadísticas descriptivas, test de normalidad (Shapiro willks), varianza (Kruskal wallis). No se observaron diferencias estadísticamente significativas (SSD) entre los focos de calor e incendios ocurridos en los diferentes meses o estaciones estudiadas. Se encontraron SSD entre los años 2004, 2006 y 2008, y los años 2005 y 2007. Tomando en cuenta los resultados obtenidos, los incendios del Complejo Litoral del Río Paraná, en general son de corta duración y pequeño tamaño, con un patrón primavero-estival de ocurrencia, y su número varía significativamente entre años. Períodos excepcionales, como el año 2008, pueden ser identificados por la variación de estos parámetros. Esta información se presenta como una herramienta apropiada para el manejo del fuego en la región.  ","palabras_clave":"MODIS|Humedal|Río Paraná|CONAE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64618,"idioma_id":4,"titulo":"TLR7 Triggering with Polyuridylic Acid Promotes Cross-Presentation in CD8a+ Conventional Dendritic Cells by Enhancing Antigen Preservation and MHC Class I Antigen Permanence on the Dendritic Cell Surface.","resumen":"ssRNA can interact with dendritic cells (DCs) through binding to TLR7, inducing secretion of proinflammatory cytokines and type I  IFN. Triggering TLR7 enhances cross-priming of CD8+ T cells, which requires cross-presentation of exogenous Ag to DCs.  However, how TLR triggering can affect Ag cross-presentation is still not clear. Using OVA as an Ag model, we observed that  stimulation of TLR7 in DCs by polyuridylic acid (polyU), a synthetic ssRNA analog, generates a strong specific cytotoxic response  in C57BL\/6 mice. PolyU stimulate CD8a+ DCs to cross-prime naive CD8+ T cells in a type I IFN?dependent fashion. This  enhanced cross-priming is accompanied by a higher density of OVA256-264\/H-2Kb complexes on CD8a+ DCs treated with polyU,  as well as by upregulation of costimulatory molecules and increased secretion of proinflammatory cytokines by DCs. Crosspriming  of CD8+ T cells by DCs treated with polyU requires proteasome and Ag translocation to cytosol through the Sec61  channel in DCs. The observed enhancement in OVA cross-presentation with polyU in DCs could be mediated by a limited Ag  degradation in endophagosomal compartments and a higher permanence of OVA peptide\/MHC class I complexes on DCs.  These observations clearly reveal that key steps of Ag processing for cross-presentation can be modulated by TLR ligands,  opening new avenues for understanding their mechanisms as adjuvants of the immune response","palabras_clave":"TLR-7|DENDRITIC CELLS|CROSS-PRESENTATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64619,"idioma_id":4,"titulo":"Characterization and expression analysis of SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes in sexual and apomictic Paspalum notatum","resumen":"The SOMATIC EMBRYOGENESIS RECEPTOR LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression patterns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indicated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apomictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.","palabras_clave":"Paspalum notatum|SERK|apomixis|apospory"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64620,"idioma_id":4,"titulo":"Spectroscopic behavior of loratadine and desloratadine in different aqueous media conditions studied by means of TD-DFT calculations","resumen":"In this study, we explained the influence of the stepwise protonation of two antihistaminic drugs on their experimental absorption spectra. We demonstrated the capability of the TD-CAM-B3LYP method, combined with a polarizable continuum model, to produce good performance for the calculated spectra. The lowest energy transitions and the molecular orbital plots were analyzed in detail. The calculated UV spectra are proposed as potential alternatives to initialize the well-known MCR?ALS algorithm, especially when the spectra of the pure analytes are not available. Moreover, it can be a useful strategy for planning an experimental methodology oriented to multiway analysis when the drug species exhibit acid?base properties.","palabras_clave":"Loratadine|Desloratadine|UV Spectroscopy|Time-dependent density functional calculations|Protonation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64621,"idioma_id":4,"titulo":"Harnessing apomictic reproduction in grasses: what we have learned from Paspalum","resumen":"Background: Apomixis is an alternative route of plant reproduction that produces individuals genetically identical to the mother plant through seeds. Apomixis is desirable in agriculture, because it guarantees the perpetuation of superior genotypes (i.e. heterotic hybrid seeds) by self-seeding without loss of hybrid vigour. The Paspalum genus, an archetypal model system for mining apomixis gene(s), is composed of about 370 species that have extremely diverse reproductive systems, including self-incompatibility, self-fertility, full sexual reproduction, and facultative or obligate apomixis. Barriers to interspecific hybridization are relaxed in this genus, allowing the production of new hybrids from many different parental combinations. Paspalum is also tolerant to various parental genome contributions to the endosperm, allowing analyses of howsexually reproducing crop species might escape fromdosage effectsin the endosperm.    Scope: In this article, the available literature characterizing apomixis in Paspalum spp. and its use in breeding iscritically reviewed. In particular, a comparison is made across species of the structure and function of the genomic region controlling apomixis in order to identify a common core region shared by all apomictic Paspalum species and where apomixis genes are likely to be localized. Candidate genes are discussed, either as possible genetic determinants (including homologs to signal transduction and RNA methylation genes) or as downstream factors (such as cell-to-cell signalling and auxin response genes) depending, respectively, on their co-segregation with apomixis or less. Strategies to validate the role of candidate genes in apomictic process are also discussed, with special emphasis on plant transformation in natural apomictic species.","palabras_clave":"apomixis|comparative mapping|Paspalum|transcriptomic analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64622,"idioma_id":4,"titulo":"A melissopalynological study of artisanal honey produced in Catamarca (Argentina)","resumen":"Honey samples from apiaries located in the province of Catamarca, Argentina, were analysed. Most of the territory is arid and covered by mountains (80%). Beekeeping is situated in the arid Chaco and Yunga regions. A total of 86 pollen types, belonging to 39 families, were identified from the honey samples and 68 (79%) were native flora. Ten types were classified as predominant pollen types and were present in the monofloral honeys (n=18), Prosopis spp., Cercidium praecox, Larrea divaricata and Tournefortia lilloi are those with the highest frequency. Pollen from Fabaceae and Asteraceae has the greatest representation in honey, with 14 and 11 types, respectively. Most of the honey samples correspond to groups II (normal pollen grain content, 39%) and III (rich in pollen grain content, 30%). The honeys analysed had a typical pollen array ofarid Chaco and southern Andean Yungas flora. The present research has ontributed to describe new types of monofloral honeys from native plants. This finding points to the potential commercial worth of ethnobotanical studies in the honey market and may help future investigations, which need to establish conservation categories for endemic species.","palabras_clave":"pollen analysis|monofloral honeys|Chaco region|Yunga region"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64623,"idioma_id":5,"titulo":"La ética y algunos aspectos de la producción científica","resumen":"El progreso en la ciencia significa acumulación del conocimiento; las generaciones sucesivas de investigadores construyen su trabajo sobre la base de los resultados alcanzados por científicos anteriores. El conocimiento resultante es de este modo de uso colectivo, lo que exige de ciertas normas internas de las comunidades científicas que garanticen una conducta responsable en la investigación. Por lo que es muy importante que cada disciplina elabore su código de ética, el cual indique los parámetros mínimos que debe cumplir todo trabajo de investigación. El objetivo de este trabajo es determinar cuáles son los aspectos más frecuentes que se dan en las producciones científicas en el campo de las ciencias agropecuarias y que no están de acuerdo a la ética de una investigación científica. Se realizó una revisión crítica de numerosos artículos de investigación, de revistas científicas y de libros de actas de distintas reuniones científicas. Se detectaron diversos aspectos, tales como autoplagios, multiautoría, criterios inadecuados para la aceptación de trabajos de investigación en reuniones científicas, entre otros. Los cuales intervienen e influyen enla generación y producción de conocimiento científico; y dependen de la conducta del investigador, donde algunos de estos desaciertos pueden cometerse por desconocimiento, y otros son ajenos a la tarea del investigador pero que si le atañen como parte integrante de la comunidad científica. El investigador tiene la obligación y la responsabilidad moral de buscar la verdad científica por sí misma y que no influya en su producción intereses personales o institucionales. La ética y la moral deben ser las instancias constitutivas e indeclinables de la formación de los sujetos que intervienen en el proceso de generación de conocimiento independientemente de la tarea que realicen.","palabras_clave":"PRODUCCION|CIENTIFICA|ETICA|PUBLICACIONES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64624,"idioma_id":5,"titulo":"Reliability and validity of the KIDSCREEN-52 questionnaire to measure health related quality of life in the 8 to 18 year-old Argentinean population","resumen":"Introducción. El cuestionario KIDSCREEN es una medida de la calidad de vida relacionada con la salud (CVRS). Permite discriminar niveles de salud o evaluar la efectividad de intervenciones. El objetivo fue evaluar la fiabilidad y la validez de la versión argentina del cuestionario en población de 8 a 18 años de edad de la ciudad de Bahía Blanca.Población y métodos. Estudio transversal en escuelas públicas y privadas durante el año 2008 mediante un muestreo estratificado por delegación. El cuestionario fue autoadministrado e incluyó el KIDSCREEN (52 ítems) y preguntas sobre edad, sexo y el nivel socioeconómico (NSE) de la familia. Se analizó la fiabilidad aplicando técnicas de análisis psicométrico clásicas, así como la teoría de respuesta al ítem. La estructura factorial fue analizada mediante un análisis factorial confirmatorio (AFC) y la validez de constructo, comparando las puntuaciones medias de las dimensiones según edad, sexo y NSE.Resultados. La mayoría de las dimensiones presentaron un alfa de Cronbach &gt;0,7 y el 80% de los ítems, valores de infit o de outfit suficientes (&lt;0,8). El AFC mostró un adecuado ajuste de los datos a la estructura de diez dimensiones. Las medias de las dimensiones confirmaron puntuaciones menores en adolescentes que en niños\/as y menores en mujeres que en varones, como se esperaba en varias dimensiones de la CVRS.Conclusiones. La versión argentina del KIDSCREEN-52 presentó una aceptable fiabilidad y validez por lo que puede recomendarse su utilización en población argentina de 8 a 18 años.","palabras_clave":"Niños |Calidad de vida|Cuestionarios|Validez de las pruebas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64625,"idioma_id":5,"titulo":"La experiencia de continuidad con el proveedor de atención primaria en escolares","resumen":"Objetivo. Conocer la experiencia de continuidad de escolares según sus cuidadores y analizar la asociación según tipo de proveedor de Atención Primaria de la Salud (APS) (médico o centro) y su financiamiento (público versus de obra social o privado). Métodos. Estudio transversal en cuatro escuelas primarias de la ciudad de Córdoba (Argentina) en 2011. Los cuidadores de los niños de 9 a 13 años, respondieron un cuestionario autoadministrado que incluyó el instrumento PCAT (Primary Care Assesment Tool) para medir la función de continuidad (subdimensiones afiliación y continuidad interpersonal) y preguntas sobre características sociodemográficas y del proveedor. Se obtuvieron puntuaciones medias entre los ítems que componen la dimensión con un valor óptimo de &#8805;3. Resultados. El 89% de quienes respondieron fueron las madres de los niños. El proveedor de APS fue un centro de salud, hospital o clínica (79,9%), de financiamiento público (60,5%). Las puntuaciones medias del grado de afiliación al proveedor, fueron de 3,30 y 3,27 (p=0,86) cuando se referían a un médico y a un centro respectivamente; y de la continuidad interpersonal fue de 3,47 y 3,09 (p= 0,01). Entre quienes identificaron un centro, la media del grado de afiliación fue 3,18 paracentros públicos y 3,51 (p=0,04) para centros de obra social o privados.Conclusiones. Los cuidadores declaran mayor grado de afiliación cuando su proveedor es un centro privado o de obra social; y mejor experiencia de continuidad interpersonal cuando identifican a un médico en vez de un centro.","palabras_clave":"ATENCIÓN PRIMARIA|NIÑOS|CALIDAD|CONTINUIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64626,"idioma_id":4,"titulo":"Predictive systems ecology","resumen":"Human societies, and their well-being, depend to a significant extent on the  state of the ecosystems that surround them. These ecosystems are changing  rapidly usually in response to anthropogenic changes in the environment.  To determine the likely impact of environmental change on ecosystems  and the best ways to manage them, it would be desirable to be able to predict  their future states. We present a proposal to develop the paradigm of  predictive systems ecology, explicitly to understand and predict the properties  and behaviour of ecological systems. We discuss the necessary and  desirable features of predictive systems ecology models. There are places  where predictive systems ecology is already being practised and we summarize  a range of terrestrial and marine examples. Significant challenges  remain but we suggest that ecology would benefit both as a scientific discipline  and increase its impact in society if it were to embrace the need to  become more predictive.","palabras_clave":"Modelling  |Systems ecology|Climate change|Ecosystem assessment"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64627,"idioma_id":4,"titulo":"Is there any information on micro-structure in microwave tomography of bone tissue?","resumen":"In this work, two-dimensional simulations of the microwave dielectric properties of models with ellipses and realistic models of trabecular bone tissue are performed. In these simulations, finite difference time  domain methodology has been applied to simulate two-phase structures containing inclusions. The results presented here show that the micro-structure is an important factor in the effective dielectric properties of trabecular bone. We consider the feasibility of using the dielectric behaviour of bone tissue to be an indicator of bone health. The frequency used was 950 MHz. It was found that the dielectric properties can be used as an estimate of the degree of anisotropy of the micro-structure of the trabecular tissue.  Conductivity appears to be the most sensitive parameter in this respect. Models with ellipse shaped-inclusions are also tested to study their application to modelling bone tissue. Models with ellipses that  had an aspect ratio of a\/b = 1.5 showed relatively good agreement when compared with realistic models of bone tissue. According to the results presented here, the anisotropy of trabecular bone must be accounted  for when measuring its dielectric properties using microwave imaging.","palabras_clave":"FDTD simulation|Bone dielectric properties|Microwave tomography|Effective permittivity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64628,"idioma_id":4,"titulo":"Physiological concentrations of unconjugated bilirubin prevent oxidative stress-induced hepatocanalicular dysfunction and cholestasis","resumen":"We demonstrated that oxidative stress (OS), a key feature in most hepatopathies, induces cholestasis by actin cytoskeleton disarrangement and further endocytic internalization of key canalicular transporters, via Ca2+-dependent protein kinase C (cPKC)activation. Here, we evaluated the capability of physiological concentrations of unconjugated bilirubin (UB) to limit OS and the impairment in biliary secretory function induced by the model pro-oxidant agent, tert-butylhydroperoxide (tBuOOH).  RESULTS: UB fully prevented the formation of reactive oxygen species (ROS) and membrane lipid peroxidation induced by tBuOOH in isolated rat hepatocytes. In the isolated rat hepatocyte couplet model, physiological concentrations of UB (17.1 µM) prevented the endocytic internalization of Bsep and Mrp2 and the impairment in their secretory function induced by tBuOOH. UB also prevented actin disarrangement, as evaluated by both plasma membrane bleb formation and actin fluorescent staining. Finally, UB prevented tBuOOH-induced cPKC activation. Experiments in isolated perfused rat livers showed that physiological concentrations of UB prevent tBuOOH-induced increase in oxidized glutathione excretion and the drop in bile flow and the biliary excretion of Bsep and Mrp2 substrates.  INNOVATION: We revealed for the first time a physiological role for unconjugated bilirubin in preventing OS-induced cholestasis by a mechanism that involve bilirubin antioxidant properties.  CONCLUSION: Physiological concentrations of UB are sufficient to prevent the biliary secretory failure induced by OS, by counteracting actin ROS formation and the further actin disarrangement and internalization of canalicular transporters relevant to normal bile formation. This reveals an important role for UB in preserving biliary secretory function under OS conditions.","palabras_clave":"UNCONJUGATED BILIRUBIN |OXIDATIVE STRESS|CHOLESTASIS |TBOOH"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64629,"idioma_id":5,"titulo":"A Practical Approach for Segmentation of Twigs on Samples of Yerba Mate by Digital Image Processing","resumen":"This paper discusses the implementation of the digital image processing techniques as a method for estimating the contents of twigs in samples of yerba mate. The hardware used in this paper, to obtain the analyzed images, consists of a desktop scanner and a personal computer. Then, using the designed and developed software for this purpose, the digital images obtained were processed and analyzed using a basic segmentation technique such as binarization with histogram thresholding. This paper compares two methods for estimating weight ratios from the binarized image. Preliminary experimental results using the proposed techniques, suggest that it may be estimated with an error less than 4% the percentage of twigs in a given batch of images. The speediness, simpleness and efficiency of the test, compared with current techniques, as well as, the reduced cost of the associated hardware, presents a viable, inexpensive and straightforward","palabras_clave":"DIGITAL IMAGE PROCESSING|YERBA MATE|QUALITY CONTROL|IRAM 20514|SCANNER|SEGMENTATION|HISTOGRAM THRESHOLDING|BINARIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64630,"idioma_id":4,"titulo":"Combined treatment with benznidazole and allopurinol in mice infected with a virulent Trypanosoma cruzi isolate from Nicaragua.","resumen":"We evaluated the effect of chemotherapy with a sequential combined treatment of a low dose of benznidazole and allopurinol, in different schedules of administration, in experimental models of acute and chronic Trypanosoma cruzi infection. Mice were infected with Nicaragua T. cruzi isolate, a virulent parasite from an endemic area of Nicaragua, genotyped as TcI (Grosso et al. 2010). We assessed survival rate, IgG levels, histopathological studies and quantified parasitaemia. A 15% survival rate was recorded in untreated mice during the acute phase of T. cruzi infection. Allopurinol administered immediately after benznidazole treatment was able to reduce parasitaemia and attenuate tissue damage by reducing inflammation. Trypanosoma cruzi-specific antibodies also decreased in 40-50% of the treated mice. The addition of allopurinol during the chronic phase showed the highest beneficial effect, not only by reducing parasitaemia but also by lowering the degree of inflammation and fibrosis.","palabras_clave":"Treatment|Benznidazol|Allopurinol|mice"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64631,"idioma_id":4,"titulo":"URSODEOXYCHOLIC AND DEOXYCHOLIC ACIDS: A GOOD AND A BAD BILE ACID FOR INTESTINAL CALCIUM ABSORPTION","resumen":"The aim of this study was to investigate the effect of ursodeoxycholic acid (UDCA) on intestinal Ca2+  absorption and to find out whether the inhibition of this process caused by NaDOC could be prevented  by UDCA. Chicks were employed and divided into four groups: (a) controls, (b) treated with 10 mM  NaDOC, (c) treated with 60 lg UDCA\/100 g of b.w., and (d) treated with 10 mM NaDOC and 60 lg  UDCA\/100 g of b.w. UDCA enhanced intestinal Ca2+ absorption, which was time and dose-dependent.  UDCA avoided the inhibition of intestinal Ca2+ absorption caused by NaDOC. Both bile acids altered protein  and gene expression of molecules involved in the transcellular pathway of intestinal Ca2+ absorption,  but in the opposite way. UDCA aborted the oxidative stress produced by NaDOC in the intestine. UDCA  and UDCA plus NaDOC increased vitamin D receptor protein expression. In conclusion, UDCA is a beneficial  bile acid for intestinal Ca2+ absorption. Contrarily, NaDOC inhibits the intestinal cation absorption  through triggering oxidative stress. The use of UDCA in patients with cholestasis would be benefited  because of the protective effect on the intestinal Ca2+ absorption, avoiding the inhibition caused by  hydrophobic bile acids and neutralizing the oxidative stress.","palabras_clave":"UDCA|NADOC|INTESTINAL CA2+ ABSORPTION|CA2+- ATP ASE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64632,"idioma_id":5,"titulo":"La vesión en inglés de nuestra revista","resumen":"Editorial que trata sobre que la RFCM-UNC comenzará a ser editada en idioma inglés y castellano gracias a un convenio realizado con la Facultad de Lenguas de la UNC.","palabras_clave":"Versión|Inglés"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64633,"idioma_id":5,"titulo":"Gastroenteropatía perdedora de proteínas y posible relación con una infección por citomegalovirus. Presentación de un caso de enfermedad de Ménétrier infantil","resumen":"La enfermedad de Ménétrier infantil es una gastroenteropatía perdedora de proteínas caracterizada por hipertrofia de la mucosa gástrica, de causa desconocida, aunque la mayoría de los casos informados se han asociado a infecciones virales. Se manifiesta clínicamente por edema y bioquímicamente por hipoproteinemia e hipoalbuminemia. Es poco común en los niños, en quienes tiene un carácter benigno y autolimitado, a diferencia de los adultos, en los que tiende a cronificarse y, en ocasiones, a malignizarse. Se presenta un caso de enfermedad de Ménétrier infantil con síndrome ascítico edematoso posiblemente asociado a una infección por citomegalovirus.","palabras_clave":"enfermedad de Ménétrier, | gastroenteropatía perdedora de proteínas, |síndrome ascítico edematoso,|citomegalovirus."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64634,"idioma_id":5,"titulo":"¿Se contagian las conductas suicidas en los jóvenes?","resumen":"Este estudio aporta suficiente evidencia de que la exposición al suicidio entre los jóvenes, por sí sola, es un factor de riesgo de conductas y comportamientos suicidas en el futuro.","palabras_clave":"Suicidio|Adolescentes"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64635,"idioma_id":4,"titulo":"Wingless-type family member 3A triggers neuronal polarization via cross-activation of the insulin-like growth factor-1 receptor pathway","resumen":"Initial axonal elongation is one of the hallmarks of neuronal polarization. This phenomenon requires polarized activation of IGF-1 receptors (IGF-1r) and subsequent activation of the phosphatidylinositol 3 kinase (PI3k) pathway. Growth factors belonging to the wingeless-type family (Wnts) have been also implied in the regulation of axonal development. It is not known, however, if Wnts have any participation in the regulation of initial axonal outgrowth and the establishment of neuronal polarity. Here, we used cultured hippocampal pyramidal neurons and growth cone particles (GCPs) isolated from fetal rat brain to show that stimulation with the wingless family factor 3A (Wnt3a) is sufficient to promote neuronal polarization in the absence of IGF-1 or high levels of insulin. We also show that Wnt3a triggers the activation of IGF-1r and PI3k (polarized to one minor neurite) in neurons in stage 2 of development (i.e., not polarized). In addition, we show that the presence of activatable IGF-1 receptors and PI3k activation are necessary for Wnt3a polarizing effects. Surface plasmon resonance (SPR) experiments showed that Wnt3a does not bind specifically to the IGF-1r. Using crosslinking and immunoprecipitation experiments, we show that, in GCPs, stimulation with Wnt3a triggers the formation of a complex including IGF1r (active)-Wnt3a-Frizzled-7. We conclude that Wnt3a triggers polarization of hippocampal neurons via cross-activation of the IGF-1 receptor\/PI3k pathway.","palabras_clave":"Wnt3a|IGF-1 receptors|neuron differentiation|polarity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64636,"idioma_id":4,"titulo":"Friendly Strategy to Prepare Encoded One bead-One Compound Cyclic Peptide Library","resumen":"One-bead-one-peptide libraries allow the screening of suitable ligands for any target protein. Short cyclic peptides are ideal ligands for affinity chromatography due to their high affinity and selectivity for the target protein and stability against proteases. We designed a library synthesis strategy to facilitate the identification of cyclic peptides by MS consisting in: a) sequential incorporation of a mixture of Fmoc-Ala-OH and Fmoc-Asp[2-phenylisopropyl (OPp)]- OH (15:85) to Gly-hydroxymethylbenzoyl-ChemMatrix resin, b) synthesis of the combinatorial library on the resin by the divide-couple-recombine method, c) removal of OPp with 4% TFA d) peptide cyclization on solid phase through side-chain Asp and amino terminus, e) removal of side chain protecting groups with a 95% TFA cocktail. Peptides were cleaved from the beads with ammonia and the linear code was sequenced by MALDI-TOF MS\/MS. The high capacity of ChemMatrix resin together with the sensitivity of MS allows code sequencing from a single bead.    Small peptides consisting of a few amino acids represent promising affinity ligands candidates for industrial separations by affinity chromatography. Peptide ligands are much more physically and chemically stable and resistant to proteolytic cleavage than antibodies. They can be readily synthesized by standard chemistry in bulk amounts at a lower cost under good manufacturing practices. Furthermore, peptides allow site-directed immobilization and high ligand density and the matrices are more robust during elution and regeneration than protein-based affinity matrices such as monoclonal antibodies. Particularly, cyclic peptides have higher selectivity and resistance to enzymatic degradation than linear peptides due to their structural constraint which reduces the conformational flexibility, thus potentiating their use as affinity ligands for protein purification.  The application of combinatorial peptide synthesis strategies greatly facilitates the discovery of suitable ligands for any given protein of interest. Among the solid-phase strategies available for peptide library preparation, the divide-couple-recombine (DCR), also known as the split-and-mix method, is the most advantageous. This approach entails the following: (i) dividing the solid support (resin beads) into equal portions; (ii) coupling each portion individually with a different amino acid (building block); and (iii), mixing the portions. This procedure assures a theoretically even representation of the library members and a ?one-bead-one-peptide? distribution. To screen these combinatorial libraries, tens of thousands to millions of compound beads are first mixed with the probe molecule. The beads that interact with it will be identified and isolated for peptide structure determination.  The high sensitivity and mass accuracy of mass spectrometry (MS), together with the speed of the analyses and the large amount of information generated in each experiment made it a technique of choice for peptide sequencing. Although linear peptides may be readily sequenced by MS, cyclic peptides typically yield complex fragment ion mass spectra, arising from ring opening at multiple positions to afford mass degenerate ions, which undergo subsequent loss of residues. One-bead-two-peptide libraries in which each bead contains both the cyclic peptide and its linear counterpart as a coding tag facilitate MS analysis. For peptide identification by MS a cleavage site to release the peptides from the beads is required. The cyclic peptide as well as its linear counterpart anchored on each bead are released and analyzed together by MS. In current approaches, methionine is commonly used as linker.8-11 The main drawback is that the selective cleavage of the peptides is performed with CNBr which is a very hazardous reactive, especially when working with hundreds of individual beads selected from the screening process.12 We have previously proposed the use of 4-hydroxymethylbenzoic acid (HMBA) linker13 coupled to ChemMatrix (CM) resin for one-bead-one-peptide library synthesis14 and we have developed a suitable cleavage method to release the peptide from the linker HMBA by vapor phase ammonolysis using NH4OH 30%, which is much more economic and safer than CNBr. The method allows MS analysis from each single bead isolated after one-bead-one-peptide library screening.   The aim of this work was to design a new strategy to prepare beads with homodetic cyclic peptides together with a minimum proportion of its linear peptide code suitable for one-bead-one-peptide libraries. The method was based on the proper combination of the solid support, the linker for resin-peptide anchorage and protecting groups for N-alfa and side-chain protection.   CM was used as solid support and HMBA as linker. CM is a highly crosslinked, amphiphilic resin composed entirely of polyethyleneglycol monomers that contain exclusively primary ether bonds. These bonds facilitate high resin loading, as compared to polystyrene resins, and high chemical and mechanical stability. CM is compatible with both organic and aqueous solvents, which are used for the peptide synthesis and for the screening step respectively, and therefore it is highly suitable for the whole process. The benzyl ester, which forms the HMBA linker with the first amino acid, is stable to piperidine used for Fmoc group removal making the linkage applicable for chemical elongation of a peptide sequence with Fmoc strategies. The HMBA inertness to trifluoroacetic acid (TFA) allows side-chain deprotection without releasing the peptide from the resin. The removal of protecting groups is required since screening is normally carried out on fully unprotected peptides. At the C-terminal, Gly was incorporated with the aim of increasing the molecular weight of the final peptide to facilitate its identification by MS and overcoming the poor cleavage efficiency of esters of Ile and Val.  For solid phase ring formation Fmoc-Asp[2-phenylisopropyl (OPp)]-OH was added to the peptide sequence. The semi-permanent group OPp was cleaved with 4% TFA and an amide linkage was formed between the amino terminus and carboxylic acid function to give homodetic cyclic peptides. Most permanent protecting groups commonly used in solid phase peptide synthesis are stable to 4% TFA. However, these conditions are sufficient to remove the side-chain trityl (Trt) protection of His residue. Thereafter, in the case of using His in the peptide library, Fmoc-His(Boc)-OH must be used instead of Fmoc-His(Trt)-OH. 4-[N-(l-(4,4-dimethyl-2,6-dioxocyclohexylidene)-3-methylbutyl)amino]-benzyl (ODmab), commonly used as a semi-permanent protecting group for Asp in solid phase cyclization, cannot be used in the present approach because its cleavage is achieved with hydrazine, reactive which also removes the peptides from the HMBA-CM resin.   In order to prepare a peptide code together with the cyclic peptide, a minor proportion of Fmoc-Ala-OH was incorporated instead of Fmoc-Asp(OPp)-OH. For the purpose of reducing interference during the screening step due to the interaction of the linear peptides with the target, the minimum proportion of the linear code necessary for its MS analysis was synthesized. When adding 0.2 eq of Fmoc-Ala-OH to the resin previous to Fmoc-Asp(OPp)-OH incorporation, only 0.15 eq of Fmoc-Ala-OH was incorporated as was measured by Fmoc analysis. Then, Fmoc-Asp(OPp)-OH was coupled to the remaining resin equivalents.   Peptides bounded through HMBA to the resin beads can be released with nucleophiles. CM contains exclusively primary ether bonds and so is stable to nucleophilic conditions. The ammonia vapor has the advantage over other nucleophiles such as NaOH that it is easily removed by evaporation, thus avoiding the presence of contaminants that could interfere with the MS peptide analysis.   The small proportion of the code (non-cycled Ala containing peptide) was enough for MS analysis due to the high sensitivity of the MS technique together with the high loadings of HMBA-CM (0.67 mmol\/g substitution). High quality mass spectra were obtained. Peptide signals were high and the cyclic peptide sequence could be deduced from the tandem mass spectra of the linear peptide. Figure 1 shows the resulting MALDI-mass spectrum and MALDI-tandem mass spectrum performed, obtained by adding 1 &#956;L of the eluted peptides ARYKSRVAG-NH2 and ciclocyclo-(1,8)-ARYKSRVDG-NH2 from a single bead.   To prepare the linear code Fmoc-Asp(tBu)-OH together with Fmoc-Asp(OPp)-OH could have been used instead of Fmoc-Ala-OH but the second amino acid was cheaper. Moreover, when preparing a cyclic peptide library of tens of thousands to millions of compounds, the yield of cyclization of some minor sequences may be incomplete but undetected by the Kaiser test. If Fmoc-Asp(tBu)-OH together with Asp(OPp)-OH is used, it is not possible to be aware of incomplete cyclization of the peptide-bead analyzed specially because MS is not quantitative. Besides, when using Fmoc-Ala-OH together with Fmoc-Asp(OPp)-OH, incomplete cyclization can be clearly visualized with MS because there will be three different signals corresponding to the cyclic peptide, its linear counterpart and its linear code clearly identified by MS.   The strategy developed is suitable for the synthesis of one-bead-one-peptide libraries in which each bead has a cyclic peptide together with a minimum proportion of its linear code for MS identification","palabras_clave":"COMBINATORIAL CHEMISTRY|MALDI-TOF MS\/MS|PEPTIDE SEQUENCING|2-PHENYLISOPROPYL|AFFINITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64637,"idioma_id":4,"titulo":"Landau level transitions in doped graphene in a time dependent magnetic field","resumen":"The aim of this work is to describe the Landau level transitions of Bloch electrons in doped graphene with an arbitrary time dependent magnetic field in the long wavelength approximation. In particular, transitions from the m Landau level to the m±1 and m±2 Landau levels are studied using the time dependent perturbation theory. Time intervals are computed in which transition probabilities tend to zero at a low order in the coupling constant. In particular, Landau level transitions are studied in the case of Bloch electrons traveling in the direction of the applied magnetic force and the results are compared with classical and revival periods of electrical current in graphene. Finally, current probabilities are computed for the n=0 and n=1 Landau levels showing expected oscillating behavior with modified cyclotron frequency.","palabras_clave":"Doped graphene | Landau levels|Time dependent magnetic field|Transition probabilities"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64638,"idioma_id":4,"titulo":"Benzene adsorption on PtCo(1 1 1): A DFT study","resumen":"Adsorption benzene on PtCo(1 1 1) surface atlow coverage is investigated using density functional theory calculations. We have investigated the PtCo FCT alloy surface with a uniform distribution and two- benzene orientation on the surface. It was found that the most favorable site is a Co Co bridge with an adsorption energy of &#8722;0.37 eV. A large buckling for the first Co and Pt atoms on the surface layers and a 17?30&#9702; H-tilt angles are found. The bonding analysis indicates that C C and C H bonding increase while Pt Pt, Co Co and Pt Co decrease 13.2, 73 and 33%, respectively, after adsorption benzene. The vibrational frequencies of adsorbed benzene were also calculated.","palabras_clave":"DFT| PtCo|BENZENE ADSORPTION|ELECTRONIC STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64639,"idioma_id":4,"titulo":"Targeting of arenavirus RNA synthesis by a carboxamide-derivatized aromatic disulfide with virucidal activity","resumen":"Several arenaviruses can cause severe hemorrhagic fever (HF) in humans,   representing a public health threat in endemic areas of Africa and South   America. The present study characterizes the potent virucidal activity   of the carboxamide-derivatized aromatic disulfide NSC4492,   an antiretroviral zinc finger-reactive compound, against Junín virus   (JUNV), the causative agent of Argentine HF. The compound was able to   inactivate JUNV in a time and temperature-dependent manner, producing   more than 99 % reduction in virus titer upon incubation with virions at   37°C for 90 min. The ability of NSC4492-treated JUNV to   go through different steps of the multiplication cycle was then   evaluated. Inactivated virions were able to bind and enter into the host   cell with similar efficiency as control infectious particles. In   contrast, treatment with NSC4492 impaired the capacity   of JUNV to drive viral RNA synthesis, as measured by quantitative   RT-PCR, and blocked viral protein expression, as determined by indirect   immunofluorescence. These results suggest that the disulfide NSC4492   targets on the arenavirus replication complex leading to impairment in   viral RNA synthesis. Additionally, analysis of VLP produced in NSC4492-treated   cells expressing JUNV matrix Z protein revealed that the compound may   interact with Z resulting in an altered aggregation behavior of this   protein, but without affecting its intrinsic self-budding properties.   The potential perspectives of NSC4492 as an inactivating vaccinal compound for pathogenic arenaviruses are discussed.","palabras_clave":"ARENAVIRUS|AROMATIC DISULFIDES|VIRUCIDAL|JUNV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64640,"idioma_id":4,"titulo":"Free Vibrations of Beam System Structures with Elastic Boundary Conditions and an Internal Elastic Hinge","resumen":"The study of the dynamic properties of beam structures is extremely important for proper structural design. This present paper deals with the free in-plane vibrations of a system of two orthogonal beam members with an internal elastic hinge. The system is clamped at one end and is elastically connected at the other. Vibrations are analyzed for different boundary conditions at the elastically connected end, including classical conditions such as clamped, simply supported, and free. The beam system is assumed to behave according to the Bernoulli-Euler theory. The governing equations of motion of the structural system in free bending vibration are derived using Hamilton´s principle. The exact expression for natural frequencies is obtained using the calculus of variations technique and the method of separation of variables. In the frequency analysis, special attention is paid to the influence of the flexibility and location of the elastic hinge. Results are very similar with those obtained using the finite element method, with values of particular cases of the model available in the literature, and with measurements in an experimental device.","palabras_clave":"Beams|Structures|Elastic Hinge|Free Vibrations|Elastic Boundary Conditions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64641,"idioma_id":5,"titulo":"¿Cuáles son los derechos humanos de las personas migrantes vigentes en Argentina?","resumen":"Los derechos humanos constituyen la ruta de navegación de muchos Estados en la actualidad. Argentina es un Estado alta parte contratante de esos instrumentos internacionales de protección. En el país coexisten dos sistemas de protección de derechos humanos (el Sistema Interamericano y el Sistema Universal) que contienen tratados internacionales con valores generales. Este escrito pretende hacer un breve recuento de las normas internacionales ratificadas por la Argentina en materia de protección de los derechos de las personas migrantes, documentando las posibles incoherencias entre los documentos de derechos humanos. Se entenderá la norma como creadora de realidades y de situaciones que perpetúan los sistemas capitalistas de producción donde las personas migrantes terminan por ser ?trabajo vivo?.","palabras_clave":"DERECHOS|HUMANOS|MIGRACIÓN|TRABAJO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64642,"idioma_id":4,"titulo":"Evaluation of the cytotoxicity, genotoxicity and apoptotic induction of an aqueous extract of Achyrocline satureioides (Lam.) DC","resumen":"Achyrocline satureioides is widely consumed as infusion or aperitif and shows important therapeutic properties. Previously, we reported absence of genotoxicity of cold aqueous extract (CAE) of A. satureioides by Allium test. However, one test cannot predict the genotoxic effects of a substance. Thus, the aim of this work was to investigate cytotoxicity, genotoxicity and apoptotic ability of CAE of A. satureioides. In addition, CAE was chemically characterized. The cytotoxicity was evaluated by Trypan blue and MTT assays. The apoptotic capacity was evaluated by Hoechst staining and DNA fragmentation-analysis. The genotoxicity was studied by comet assay (CA) and micronucleus test. The identification and quantification of flavonoids were performed by HPLC?ESI?MS\/MS. The cytotoxicity studies indicated low toxicity of CAE. In addition, CAE did not induce apoptotic effects on human PBMCs. CAE did not show genotoxicity in vitro against Vero cells, at 10?50 lg\/mL. CAE did not induce in vivo genotoxic effects, but it showed at high concentrations cytotoxicity by micronucleus assay. CAE presented flavonoids such as quercetin, 3-Omethylquercetin and luteolin. In conclusion, A. satureioides at popularly concentrations used, in aperitif or infusion, can be consumed safely because did not show any cytotoxic or genotoxic effects.is widely consumed as infusion or aperitif and shows important therapeutic properties. Previously, we reported absence of genotoxicity of cold aqueous extract (CAE) of A. satureioides by Allium test. However, one test cannot predict the genotoxic effects of a substance. Thus, the aim of this work was to investigate cytotoxicity, genotoxicity and apoptotic ability of CAE of A. satureioides. In addition, CAE was chemically characterized. The cytotoxicity was evaluated by Trypan blue and MTT assays. The apoptotic capacity was evaluated by Hoechst staining and DNA fragmentation-analysis. The genotoxicity was studied by comet assay (CA) and micronucleus test. The identification and quantification of flavonoids were performed by HPLC?ESI?MS\/MS. The cytotoxicity studies indicated low toxicity of CAE. In addition, CAE did not induce apoptotic effects on human PBMCs. CAE did not show genotoxicity in vitro against Vero cells, at 10?50 lg\/mL. CAE did not induce in vivo genotoxic effects, but it showed at high concentrations cytotoxicity by micronucleus assay. CAE presented flavonoids such as quercetin, 3-Omethylquercetin and luteolin. In conclusion, A. satureioides at popularly concentrations used, in aperitif or infusion, can be consumed safely because did not show any cytotoxic or genotoxic effects.","palabras_clave":"ACHYROCLINE SATUREIOIDES (LAM.) DC|COLD AQUEOUS EXTRACT|CYTOTOXICITY|GENOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64643,"idioma_id":4,"titulo":"Optimal design of passive viscous damping systems for buildings under seismic excitation","resumen":"Nowadays, it is known that through the use of energy dissipation devices, the seismic performance of buildings can be improved. However, for efficiency and structural safety, the locations and sizes of these devices need to be properly defined. In this work, a procedure to optimally define the damping coefficients of added linear viscous dampers tomeet an expected level of performance on buildings under seismic excitation is proposed. The performance criterion is expressed in terms of a maximum interstory drift, which is one of themost important limitations provided by the seismic design codes. For a given level of performance, the effectiveness of the damper distribution obtained by means of different objective functions is also assessed. Knowing that themain contribution to the total uncertainty is due to the excitation andwith the aimof achieving robust results, the most appropriate approach to model the excitation is through a stationary stochastic process characterized by a power spectral density compatible with the response spectrum defined by the seismic design code. Accordingly, the structural response is obtained in the frequency domain. Through numerical examples, on planar and threedimensional steel buildings with coupled lateral and torsional vibrations, the proposed procedure is verified.","palabras_clave":"Optimal damper placement|Passive control|Viscous damper|Stochastic analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64644,"idioma_id":4,"titulo":"Evaluation of biochemical markers in the golden mussel Limnoperna fortunei exposed to glyphosate acid in outdoor microcosms","resumen":"In this study, the impact of technical grade glyphosate acid on Limnoperna fortunei was assessed employing outdoor microcosms treated with nominal glyphosate concentrations of 1, 3 and 6 mg L-1. At the end of the experiment (26 days), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), acetylcholinesterase (AChE), carboxylesterases (CES) and alkaline phosphatase (ALP) activities, and lipid peroxidation levels were analyzed. GST and ALP activities and lipid peroxidation levels showed a significant increase with respect to controls in the mussels exposed to glyphosate (up to 90%, 500% and 69%, respectively). CES and SOD activities showed a significant decrease in glyphosate exposed bivalves with respect to controls (up to 48% and 37%, respectively). CAT and AChE did not show differences between exposed and no exposed bivalves. The increase in lipid peroxidation levels and the decrease in SOD and CES activities observed in L. fortunei indicate that glyphosate had adverse effects on the metabolism of this bivalve. The results of the present study also indicate that a \u201Cmultibiomarker approach\u201D provides a more precise knowledge of the impact of glyphosate on L. fortunei.","palabras_clave":"GLYPHOSATE|LIMNOPERNA FORTUNEI|GOLDEN MUSSEL|BIOMARKERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64645,"idioma_id":4,"titulo":"Effect of seasonality on oxidative stress responses and metal accumulation in soft tissues of Aulacomya atra, a mussel from the South Atlantic Patagonian coast","resumen":"This study investigated the effects of pollution and its interaction with temperature on the oxidative status of the ribbed mussel Aulacomya atra in the southern Atlantic Patagonian coast. Animals were collected from four sites with different degree and type of human activity impact, during the summer and winter of 2011. Seawater chromium, copper, manganese, nickel and zinc concentrations were measured, as well as metal accumulation, lipid peroxidation, protein oxidation, reduced glutathione levels, and enzymatic activities of superoxide dismutase and glutathione-S- transferase in gills and digestive glands.   Metal bioacumulation and oxidative stress responses in both tissues were generally higher in mussels from harbor areas. Water temperature had a remarkable effect on gill SOD activity and protein oxidation during winter in mussels from all locations.  Methodologically, we conclude that measuring both metal bioaccumulation and oxidative stress responses allowed for a more accurate assessment of the biological effects of metal present in seawater.","palabras_clave":"Aulacomya atra |Trace metals |Metal bioaccumulation |Temperature effects|Oxidative stress |Antioxidant defenses"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64646,"idioma_id":4,"titulo":"Hydrostatic-pressure dependence of Raman-active optical phonons in Nd:Mg:LiNbO3","resumen":"In this work, we report the frequency variation of Raman phonon modes of a doped Nd:Mg:LiNbO3 crystal under high hydrostatic pressure up to 6.5 GPa. Whereas most of the phonon modes are not affected by doping, showing the same pressure dependence of their frequency as for undoped LiNbO3, there are three exceptions: The A1(TO)2, the A1(TO)3 and the E(TO)4 phonons. These modes exhibit a considerably different pressure coefficient, which is mainly attributed to the influence of Mg ions occupying Nb-sites in the lithium niobate matrix.","palabras_clave":"Espectroscopia Raman|Presion Hidrostatica|Niobato de Litio|Magnesio"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64647,"idioma_id":4,"titulo":"Hydrogen embrittlement of a Fe-Cr-Ni alloy: analysis of the physical   and chemical processes in the       early stage of stress corrosion cracking initiation","resumen":"En este capítulo se estudió mediante el método de superposición atómica y deslocalización electrónica de orbitales moleculares (ASED-MO) la interacción de dos átomos de hidrógeno en una zona de una vacancia (V) de la aleación &#947;-Fe55Cr25Ni20. Se determinó que las impurezas se sitúan y alinean a lo largo de la dirección [1 0] y con la vacancia, en el plano (111). Este comportamiento podría estar relacionado con una clusterización de vacancias lineales de hidrógeno, como un paso previo al inicio de la grieta. La estructura electrónica de los átomos de Fe, Cr y Ni cerca de la vacancia, se modifica  después de la ubicación del hidrógeno. Las interacciones involucran esencialmente a los orbitales atómicos Fe 4px y Cr 4py. Así los orbitales metálicos 3dx2-y2, 3dz2, 3dz2 y 3dxz tienen también participación en las interacciones aleación-hidrógeno. La ubicación del hidrógeno produjo una transferencia de electrones a los átomos de H desde los átomos vecinos más cercanos Fe, Cr y Ni, la que contribuye a formar los nuevos enlaces metálicos H-metal. Se determinó que los enlaces metal-metal se debilitaron para dar lugar a la formación de los nuevos pares de H-Fe, Cr-H, y H-Ni. De esta forma los átomos de Cr tienen un papel importante en el proceso de fragilización; los enlaces fuertes Cr-Fe, Cr-Cr y Cr-Ni son los más afectados, concluyendo  que la interacción H-Cr tiene la mayor población de solapamiento. Una interacción H-H  podría estar asociada con el precursor de la burbuja de hidrógeno, pero está lejos de ser un enlace típico de la molécula de H. Los procesos físicos y químicos citados en este capítulo  juegan un papel clave en la posterior nucleación de la corrosión localizada tales como la iniciación de la corrosión bajo tensión.","palabras_clave":"Superaleaciones|Hornos|Carburos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64648,"idioma_id":5,"titulo":"El aeropolen en la ciudad de Bahía Blanca (Argentina): Aportes para la gestión del arbolado público desde la selección de especies","resumen":"La localidad de Bahía Blanca, ubicada en la provincia de Buenos Aires, se halla inserta en un espacio ecotonal entre el monte, la pradera y el espinal. En esta ciudad la mayor parte del arbolado urbano de alineación se encuentra compuesto por especies arbóreas exóticas que generan una influencia positiva en algunos casos y negativa en otros sobre la calidad de vida de la población. Por ello, el estudio del paisaje vegetal urbano contribuye al ordenamiento espacial en cuanto a la toma de decisiones sobre la selección de especies.Según distintos estudios existe una relación estrecha entre la especie arbórea, el clima local y el aeropolen, combinación que puede constituirse en una desventaja en ciertas épocas del año. El objetivo del presente trabajo es reconocer qué especies arbóreas no son recomendables para el arbolado urbano en la ciudad de Bahía Blanca. En principio se identificaron y contabilizaron las especies arbóreas de alineación aplicando el censo de arbolado urbano para definir su patrón de distribución.&nbsp; Además se realizó un recuento de pólenes utilizando el método de tipo volumétrico mediante el muestreador&nbsp; Rotorod desde el año 2006 al 2012. Estos últimos datos fueron recolectados diariamente por el Instituto de Alergia e Inmunología del Sur de Bahía Blanca. También se identificaron y localizaron espacialmente en la cartografía a los pacientes de dicho Instituto que sufren de alergia por el polen de los árboles. A través de la información obtenida se elaboró un mapa de vulnerabilidad. Este estudio busca contribuir al ordenamiento urbano mediante la correcta elección de las especies arbóreas que formarán parte del arbolado de alineación.","palabras_clave":"PAISAJE VEGETAL URBANO|ORDENAMIENTO URBANO|MAPA DE VULNERABILIDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64649,"idioma_id":4,"titulo":"Comparing galaxy populations in compact and loose groups of galaxies II:  brightest group galaxies","resumen":"The properties of the brightest galaxies (BCGs) are studied in both compact and loose   groups of galaxies in order to better understand the physical mechanisms influencing   galaxy evolution in different environments.  Samples of BCGs are selected in the compact groups identified by McConnachie et al. (2009), and in loose groups taken from Zandivarez & Martínez  (2011). The following physical properties of the BCGs in compact groups and in subsamples  of loose groups are compared, defined by their mass and total luminosity: absolute   magnitude, colour, size, surface brightness, stellar mass, concentration and   morphological information from the Galaxy Zoo. The fraction of BCGs classified as red   and\/or early-type as a function of galaxy luminosity are studied.   The fraction of the group´s total luminosity contained in the  BCG and the difference in luminosity between the BCG and the second-ranked galaxy, are  also analysed.  Some properties of BCGs in compact and loose groups are comparable.   However, BCGs in compact groups are systematically more concentrated and have larger   surface brightness than their counterparts in both, high- and low-mass loose groups.  The fractions of red and early-type BCGs in compact groups are consistent with those of  high-mass loose groups. Comparing BCGs in subsamples of compact and loose groups   selected for their similar luminosities, BCGs in compact groups are found to be, on   average, brighter, more massive, larger, redder and more frequently classified as   elliptical. In compact groups, the BCG contains a larger fraction of the system´s total   luminosity and differs more in absolute magnitude from the second-ranked galaxy.  Using a simple model, which dry-merges the BCG in loose groups with a random choice  among the 2nd, 3rd and 4th-ranked galaxies in the group, and allowing for some star loss  in the process, we show that the absolute magnitude distributions of BCGs in compact   and loose groups of similar luminosities can be made more alike.  BCGs in compact and loose groups are found to be different. Some mechanisms   responsible for transforming late-type galaxies into early types, such as mergers, may   be more effective within compact groups due to their high densities and small velocity   dispersion, which would lead their BCGs along somewhat different evolutionary paths.","palabras_clave":"Galaxies|Groups|Paremeters|Evolution"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64650,"idioma_id":5,"titulo":"MODELOS ANIMALES DE RETINOPATÍA DIABÉTICA: COMPRENSIÓN DE LA PATOGÉNESIS  Y POTENCIAL UTILIZACIÓN EN EL DESARROLLO DE NUEVOS AGENTES TERAPEUTICOS","resumen":"La retinopatía diabética (RD) es la complicación microvascular más común  de la diabetes y una de las principales causas de ceguera en todo el mundo. Varios  modelos animales, generados por inducción farmacológica, dietas hipocalóricas,  y de forma espontánea por endogamia selectiva o modificación genética, han sido  utilizados para investigar la patogénesis de la RD. Entre ellos, los roedores han sido los más estudiados debido a  su corto tiempo de generación, a la hiperglucemia heredada y\/o a la obesidad que afecta a ciertas cepas. En particular, los ratones han demostrado ser útiles para el estudio de las etapas  iniciales de la RD. Sin embargo, actualmente ningún modelo animal es capaz de  reproducir las complicaciones vasculares y no vasculares asociadas con las  etapas más avanzadas, encontradas en humanos. En esta revisión, intentamos  destacar las ventajas y desventajas de estos modelos y brindar información para  la planificación de estudios que apunten al mejor entendimiento de la RD así  como al desarrollo de nuevas estrategias farmacológicas útiles para su  prevención y tratamiento.      &nbsp;","palabras_clave":"retinopatia diabetica|modelos animales|patogenesis|terapeutica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64651,"idioma_id":4,"titulo":"Seasonal variation in agar composition and properties from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonian coast of Argentina","resumen":"Seasonal variation of agar from specimens of a commercially exploited population of Gracilaria gracilis (Stackhouse) Steentoft, Irvine & Farnham in the Patagonian coast of Argentina was studied. For each seasonal harvest, random samples of plants were pooled for subsequent polysaccharide extraction at different water temperatures and agar physico-chemical properties and composition were determined. Both spring and summer plants yielded 30% and 41% of agar, respectively, which differed slightly in their at rest rheological behavior. Spring and summer plants produced strong gels (238 and 218 g cm-2, respectively), but the latter gels had nil adhesiveness. In autumn plants, agar yield decayed to 19%, though the product still maintained similar gel strength (210 g cm-2). Adhesiveness in this product was at least an order of magnitude higher than in the others, concomitant with a peak in the formation of tetraspores and carpospores. This suggests a biological role for the galactan in the initial attachment of spores to the substrate. But since fall corresponds to the settling of reproductive structures, caution should be taken to harvest the algae once spores have been shed from mother plants.","palabras_clave":"GRACILARIA GRACILIS|RHEOLOGICAL BEHAVIOR|SEASONAL VARIATION|UTRASTRUCTURAL CHANGES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64652,"idioma_id":4,"titulo":"Macroevolution of panicoid inflorescences: a history of contingency and order of trait acquisition","resumen":"Inflorescence forms of panicoid grasses (Panicoideae s.s.) are remarkably diverse and they look very labile to human eyes; however, when performing a close inspection one can identify                        just a small subset of inflorescence types among a huge morphospace of possibilities. Consequently, some evolutionary constraints                        have restricted, to some extent, the diversification of their inflorescence. Developmental and genetic mechanisms, the photosynthetic                        type and plant longevity have been postulated as candidate constraints for angiosperms and panicoids in particular; however,                        it is not clear how these factors operate and which of these have played a key role during the grass inflorescence evolution.                        To gain insight into this matter the macroevolutionary aspects of panicoid inflorescences are investigated.","palabras_clave":"Inflorescence|Morphology|Panicoids|Panicoideae|Poaceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64653,"idioma_id":5,"titulo":"Plantas medicinales de los bajos alcalinos de la cuenca del arroyo del Azul (provincia de Buenos Aires, Argentina)","resumen":"El presente trabajo se llevó a cabo en 7 bajos alcalinos ubicados en el sector más llano de la cuenca del arroyo del Azul (centro de la provincia de Buenos Aires, Argentina). Dichos ambientes, debido a sus limitaciones edáficas, son destinados a la cría de ganado bovino. El objetivo del presente trabajo es analizar estas unidades del paisaje desde una perspectiva que permita valorizarlos aún más, a partir de la consideración de la gran diversidad de especies botánicas de valor medicinal que se desarrolla en ellos. Se identificaron 150 especies, de las cuales 81 (54%) resultaron con propiedades medicinales. La familia con mayor cantidad de especies con uso medicinal fue Asteraceae, seguida por Poaceae y, en menor medida, por Apiaceae y Plantaginaceae. El uso medicinal potencial más representado fue el diurético (30 especies), seguido por el digestivo (17 especies), el hepático (15 especies) y el vulnerario (13 especies). Por otro lado, más de la mitad de las especies encontradas (60,5%) presenta alguna acción vinculada con el sistema digestivo. Los ambientes analizados debieran ser considerados como proveedores de servicios ecosistémicos ya que es significativa la riqueza de su flora y la potencialidad de sus aplicaciones medicinales.","palabras_clave":"bajos alcalinos|plantas medicinales|pampa argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64654,"idioma_id":5,"titulo":"Representatividad espacial de las principales comunidades vegetales en la cuenca baja del arroyo del Azul (Buenos Aires, Argentina)","resumen":"La cuenca del arroyo del Azul (6.237 km2) se ubica en el centro de la provincia de Buenos Aires, entre 58º51´-60º10´ LO y 36º09´-37º19´ LS. La zona más deprimida de dicha cuenca  (4.500 km2) corresponde a un subambiente típico de llanura, con suelos alcalinos y\/o hidromórficos con tosca a poca profundidad, en el que se presentan diversos ambientes: pajonales de Paspalum quadrifarium, duraznillares de Solanum glaucophyllum, flechillares, pasturas degradadas, sectores cultivados, cursos de agua y sitios destinados al uso antrópico. Con el objetivo de caracterizar las principales comunidades vegetales del sector y su relevancia espacial en el mosaico paisajístico, en primer lugar se llevó a cabo el trazado de una transecta de aproximadamente 18 km en sentido perpendicular al eje principal de escurrimiento de la cuenca, en la cual se identificaron 5 comunidades vegetales principales, a partir de especies indicadoras. Se calculó la representatividad de las mismas en el espacio analizado y, posteriormente, se llevaron a cabo muestreos de vegetación durante tres primaveras consecutivas (2010-2012) que permitieron la caracterización botánico-ecológica de estos ambientes. La comunidad más representada en la transecta resultó ser la correspondiente a los llamados flechillares, ocupando un 35% del espacio analizado, los cuales conforman la matriz principal del paisaje en la cuenca baja, alternando con pajonales y pasturas degradadas. Los índices más altos de riqueza se registraron en los pajonales, mientras que los sitios más diversos resultaron ser los flechillares. La aproximación al conocimiento de la estructura del paisaje en este área típica de llanura mediante la caracterización botánico-ecológica de sus principales comunidades y su representación en el espacio, permitirá avanzar luego en el análisis de su funcionamiento, teniendo en cuenta las fuertes relaciones horizontales entre los diferentes ambientes provistas, sobre todo, por el régimen hídrico predominante.","palabras_clave":"Representatividad espacial |comunidad vegetal|Pampa deprimida"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64655,"idioma_id":5,"titulo":"Precisión y replicabilidad de una aproximación no discreta al estudio de las modificaciones artificiales del cráneo","resumen":"El objetivo principal de este trabajo es explorar la precisión y replicabilidad de las técnicas de morfometría geométrica para la descripción del patrón de variación en las modificaciones artificiales del cráneo. Nuestra aproximación exhibe su gran capacidad descriptiva y la posibilidad de transformar la variación morfológica cualitativa en información cuantitativa continua. La variación descripta es continua en el morfoespacio de las modificaciones craneanas, demostrando que los casos estudiados no conforman grupos naturalmente discretos.","palabras_clave":"BIOARQUEOLOGIA|TECNICAS CUANTITATIVAS|ERROR INTER E ITRAOBSERVADOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64656,"idioma_id":4,"titulo":"Insulin-deficient Diabetes-induced bone microarchitecture alterations are associated with a decrease in the osteogenic potential of bone marrow progenitor cells. Preventive effects of metformin","resumen":"A b s t r a c tAims: Diabetes mellitus is associated with metabolic bone disease and increased lowimpactfractures. The insulin-sensitizer metformin possesses in vitro, in vivo and ex vivoosteogenic effects, although this has not been adequately studied in the context of diabetes.We evaluated the effect of insulin-deficient diabetes and\/or metformin on bone microarchitecture,on osteogenic potential of bone marrow progenitor cells (BMPC) and possiblemechanisms involved.Methods: Partially insulin-deficient diabetes was induced in rats by nicotinamide\/strepto-zotocin-injection, with or without oral metformin treatment. Femoral metaphysis microarchitecture,ex vivo osteogenic potential of BMPC, and BMPC expression of Runx-2, PPARgand receptor for advanced glycation endproducts (RAGE) were investigated.Results: Histomorphometric analysis of diabetic femoral metaphysis demonstrated a slightdecrease in trabecular area and a significant reduction in osteocyte density, growth plateheight and TRAP (tartrate-resistant acid phosphatase) activity in the primary spongiosa.BMPC obtained from diabetic animals showed a reduction in Runx-2\/PPARg ratio and in theirosteogenic potential, and an increase in RAGE expression. Metformin treatment preventedthe diabetes-induced alterations in bone micro-architecture and BMPC osteogenic potential.Conclusion: Partially insulin-deficient diabetes induces deleterious effects on long-bonemicro-architecture that are associated with a decrease in BMPC osteogenic potential, whichcould be mediated by a decrease in their Runx-2\/PPARg ratio and up-regulation of RAGE.These diabetes-induced alterations can be totally or partially prevented by oral adminis-tration of metformin.","palabras_clave":"DIABETES MELLITUS|METFORMIN|BONE MICROARCHITECTURE|BONE MARROW PROGENITOR CELLS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64657,"idioma_id":4,"titulo":"Tilting process with humidity: DEM modelling and comparison with experiments","resumen":"Granular matter is present everywhere in ourpractical lives and mostly used in natural environment (i.e.classical atmospheric conditions). This fact implies thatthe humidity rate which controls the water content canbe involved in their behaviors. Especially adhesion forcesbetween grains are linked to this humidity rate. Here westudy a well known experiment defined as a laboratory testfor avalanches. We continuously tilt a box filled with grainsup to the appearance of precursors and full avalanches. Theseavalanches are directly proportional to the forces acting at thecontact level between grains. We use a numerical approachbased on the classical discrete element methods ?spring-Dashpot? with soft model. Firstly, we check the ability of ourcode to handle tilting experiments, then, an extra adhesionterm linked to the humidity rate is added. Full comparisonbetween ?dry? case and humid case is done.","palabras_clave":"granular instabilities|humidity|DEM code|inclination"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64658,"idioma_id":4,"titulo":"Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba","resumen":"The aim of this work was to investigate the genetic diversity, symbiotic effectiveness, drought tolerance, and indole acetic acid production of indigenous rhizobial populations in the Parque Chaqueño of Argentina able to nodulate Prosopis alba, the dominant forest tree of this region. The populations were sampled at five locations from the Arid, Semi-arid, and Humid Chaco in the Parque Chaqueño region. A set of rhizobial strains able to nodulate P. alba was obtained and selected based on their molecular diversity. Data obtained by BOX-PCR indicated that the highest molecular variability was observed in rhizobial isolates from Semi-arid Chaco. High level of indolic compound production and tolerance to osmotic treatment were significantly (p&#8201;&#8804;&#8201;0.05) correlated with water restrictions of the environments where the strains belonged. A small set of rhizobial strains that stimulate P. alba growth was selected from a large group of strains. The strains were identified by 16S rDNA sequencing as belonging to the genera Mesorhizobium, Bradyrhizobium, and Ensifer. To our knowledge, this is the first report of P. alba nodulation by strains other than Mesorhizobium chacoense, which was already described for the Parque Chaqueño.","palabras_clave":"RHIZOBIA|PROSOPIS|STRESS|DIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64659,"idioma_id":5,"titulo":"RELATOS PÚBLICOS SEDIMENTADOS EN SUJETOS LOCALES ENCUBIERTOS POR LA MEGA-MINERÍA Y EL RESURGIR DE LA RAZÓN HISTÓRICA, EN ANDALGALÁ, CATAMARCA (ARGENTINA)","resumen":"        Resumen        En la presente contribución queremos analizar la manera en  que relatos públicos vinculados a la mega-minería en Andalgalá, Catamarca  (Argentina), son sedimentados en los sujetos locales, quienes quedan  temporalmente encubiertos, borrados o escondidos ante los mismos. Desde esta  premisa describiremos cómo esos relatos o narrativas sistemáticamente  han encubierto otros puntos de vista, en este caso, el de los sujetos locales,  quienes no fueron reconocidos ni por la diferencia,  diversidad o desigualdad.         Por lo expuesto, el objetivo de este artículo consistirá en  poner en tensión la manera en que un estilo de pensamiento y conocimiento de la  realidad vinculado a la mega-minería que reconoce su origen en la modernidad, ha  contrarrestado por un período de tiempo un pensamiento diferente, limitando el  surgimiento de otras verdades.         Esta situación, que remite a las últimas dos décadas,  evidencia un cruce de racionalidades. Por un lado aparece la racionalidad  instrumental vinculada a los sectores hegemónicos, racionalidad vehiculizada en sujetos portadores de los  relatos públicos mencionados; pero, por otro, emerge otro  tipo de racionalidad, la histórica, en aquellos  sujetos que permanecieron encubiertos hasta que pudieron activar puntos  de vista diferentes.         Realizaremos este estudio desde los aportes de los  pensadores críticos latinoamericanos asociados al ?giro decolonial?, y desde lo  metodológico, nuestro análisis se apoya en la perspectiva de borde.   Palabras Claves: relatos, narrativas, racionalidad, sujetos locales, perspectiva de  borde.","palabras_clave":"relatos|narrativas|racionalidad|sujetos locales"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64660,"idioma_id":4,"titulo":"Simulations on the flow segregation problem in bidimensional piles","resumen":"We report a numerical study of the problem of flow segregation in quasi bidimensional piles built up by pouring a  mix of grains of two different sizes from a constant height. To simulate the pile formation, we employ a pseudo-dynamics  method (PSDM). The concentration of small and big grains is varied. We also try with different size ratios between the beads.  The segregation patterns arising after the pile is finished are studied by analyzing the spatial distribution of the grains. Two  different segregation indices are defined and evaluated over the piles. The conditions under which the different segregation  patterns show up are discussed. A comparison of all the results obtained with our own experimental results, is performed. The  patterns and the different conditions for the setting up of segregation are discussed. The advantages and limitations of the  simulation model are discussed.","palabras_clave":"particulate materials|segregation|heap|simulations"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64661,"idioma_id":4,"titulo":"The Migratory Capacity of Human Trophoblastic BeWo Cells: Effects of Aldosterone and the Epithelial Sodium Channel","resumen":"Aldosterone is a key regulator of ENaC channel and stimulates protein methylation on the &#946;-subunit of ENaC. We found that aldosterone (100 nM) promotes cellular migration in a wound healing model in trophoblastic BeWo cells. Here we tested if the positive influence of aldosterone on wound healing is related to the methylation reactions. Cell migration and proliferation were measured in BeWo cells at 6 h, when mitosis is still scarce. Cell migration covered 12.4, 25.3, 19.6 and 45.1 % of the wound when cultivated under control, aldosterone (12 h), 8Br-cAMP and aldosterone plus 8Br-cAMP, respectively.  Amiloride blocked the effects of aldosterone alone or in the presence of 8Br-cAMP on wound healing. Wound healing decreased in aldosterone (plus 8Br-cAMP) coexposed with the methylation inhibitor 3-deazaadenosine (3-DZA) (12.9 % reinvasion of the wound). There was an increase in wound healing  in aldosterone, 8Br-cAMP and 3-DZA- treated cells, in the presence of AdoMet, a methyl donor, compared to cells in the absence of AdoMet (27.3  and 12.9 %  reinvasion of the wound). Cell proliferation assessed with the reagent MTT was not changed in any of these treatments, suggesting that cellular migration is the main factor for the reinvasion of the wound healing. Electrophysiological studies showed an increase in ENaC current in presence of aldosterone. This effect was higher with 8Br-cAMP and there was a decrease when 3-DZA was present. AdoMet treatment partially reversed this phenomenon. We suggest that aldosterone positively influences wound healing in BeWo cells, at least in part through methylation of ENaC.","palabras_clave":"BeWo cells| wound healing|migration|ENaC|aldosterone|methylation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64662,"idioma_id":4,"titulo":"Modeling prey consumption by native and non- native piscivorous fishes: implications for competition and impacts on shared `prey in an ultraoligotrophic lake in Patagonia.","resumen":"We examined trophic  interactions of the non-native salmonids rainbow trout (Oncorhynchus mykiss), brown trout (Salmo trutta) and brook trout (Salvelinus  fontinalis) and the main native predator Creole perch (Percichthys trucha) in Lake Nahuel Huapi (Patagonia, Argentina), to  determine the relative impact of each predator on their forage base, and to  evaluate the potential vulnerability of each predator to competitive impacts by  the others. Using bioenergetics simulations we demonstrated the overall  importance of galaxiids and decapods to the energy budgets of non-native  salmonids and Creole perch. Introduced salmonids, especially rainbow trout, exerted  considerably heavier predatory demands on shared resources than native Creole  perch on both a per capita basis and in terms of relative population impacts. Rainbow  trout consumed higher quantities and a wider size range of small puyen Galaxias maculatus including early  pelagic life stages of this prey; therefore representing an additional and novel  source of mortality for the vulnerable early life stages of small puyen before and during their transition from pelagic to  benthic habitats. All  predators were generally  feeding at high feeding rates (above 40% of their maximum physiological rates) suggesting  that competition for prey does not currently limit either Creole perch or the  salmonids in this lake. This  study highlights the importance of keystone prey for the coexistence of native  species with non-native top predator. It provides new quantitative and  qualitative evidence of the high predation pressure exerted on the keystone  prey species G. maculatus during  larval to juvenile transition from pelagic to littoral-benthic habitat in  Patagonian lakes, and emphasizes the importance of monitoring salmonids and  Creole perch population dynamics in order to detect signs of potential impacts  through competition and to carefully consider the rationale behind any  additional trout stocking. ","palabras_clave":"Peces|Patagonia|Bioenergética"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64663,"idioma_id":4,"titulo":"Rotational Velocities of Southern B Stars and a Statistical Discussion of the Rotational Properties","resumen":"We have measured the projected rotational velocities of 1023 southern B stars listed in the Bright  Star Catalog (BSC) and calibrated them against the 1975 Slettebak et al. system.We joined the sample with the 1091  mostly northern stars of the BSC whose projected rotational velocities were determined in the same way. There were  387 stars in common and with the sample of 1727 B type stars we found that the published values of V sin i for B  dwarfs in the BSC average 30% higher than those derived using Slettebak et al. standards. Only 1.8% of the stars  have projected rotational velocities larger than 300 km s1 and 0.2% have V sin i larger than 350 km s1. The mean  projected rotational velocity is only 25% of break-up, implying that impending break-up is not a significant factor in  reducing rotational velocities.We have found with the largest sample used up to now that the distribution in V for all  B-type stars on the main sequence is bimodal","palabras_clave":"stars"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64664,"idioma_id":4,"titulo":"Is Gravidity 4+ a Risk Factor for Oral Clefts? A Case-Control Study in Eight South American Countries Using Structural Equation Modeling","resumen":"Background: There is disagreement about the association between cleft lip with or without cleft palate and multigravidity, which could be explained by differences of adjusting for maternal age, Amerindian ancestry, and socioeconomic status.    Objective: The aim was to evaluate gravidity 4+ (four or more gestations) as a risk factor for cleft lip with or without cleft palate in South America.    Design: We used a matched (1:1) case-control study with structural equation modeling for related causes. Data were obtained from 1,371,575 consecutive newborn infants weighing &#8805;500 g who were born in the hospitals of the Estudio Colaborativo Latinoamericano de Malformaciones Congénitas (ECLAMC) network between 1982 and 1999. There were a total of 1,271 cases with cleft lip with or without cleft palate (excluding midline and atypical cleft lip with or without cleft palate). A total of 1,227 case-control pairs were obtained, matched by maternal age, newborn gender, and year and place of birth. Potential confounders and intermediary variables were analyzed with structural equation modeling.    Results: The crude risk of gravidity 4+ was 1.41 and the 95% confidence interval was 1.14 to 1.61. When applying structural equation modeling, the effect of multigravidity on the risk of cleft lip with or without cleft palate was 1.22 and the 95% confidence interval was 0.91 to 1.39.    Conclusions: Multigravid mothers (more than four gestations) showed no greater risk of bearing children who had cleft lip with or without cleft palate than mothers with two or three births. Therefore, the often observed and reported association between multigravidity and oral clefts likely reflects the effect of other risk factors related to low socioeconomic status in South American populations.","palabras_clave":"CLEFT LIP WITH OR WITHOUT CLEFT PALATE|ECLAMC|GRAVIDITY 4+|MATCHED CASE-CONTROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64665,"idioma_id":4,"titulo":"Gastroschisis is a defect of the Umbilical ring: Evidence from Morphological evaluation of  stillborn fetuses.","resumen":"BACKGROUND: Gastroschisis (GS) is usually described as an abdominal wall defect,  to the right of a normally inserted umbilical cord, without membraneous covering  of the extruded organs. However, precise anatomical descriptions are lacking in  the literature. Our aims were to provide evidence that allows reconsideration of  its current definition, as well as an explanation for prenatal death, based on  detailed observation of stillborn fetuses with GS and a review of the literature.  METHODS: Prenatal studies, clinical examinations, and histological findings of  five stillborn fetuses with isolated GS are described and photographic evidence  is provided.  RESULTS: In all five cases, the umbilical cord was only attached to the left side  of the umbilical ring, while the right side remained uncovered, allowing  evisceration of abdominal organs. Histological evidence of mucoid-like tissue at  the free border of the ring suggests that at that site the cord was initially  inserted and later detached. Characteristics of the umbilical ring, bowel  dilatation, and autopsy findings of acute asphyxia strongly support compression  of umbilical vessels as the cause of fetal death.  CONCLUSIONS: Based on these findings, on the lack of evidence in the literature  demonstrating full-thickness abdominal wall separating the defect from the  umbilical cord, and on a critical review of the proposed mechanisms favoring the  hypothesis of a defect separate from the umbilical ring, we propose that GS  represents a failure in the normal attachment between umbilical cord and  umbilical ring. The consistent clinical course of fetuses with prenatal demise  suggests careful targeted monitoring during late gestation.","palabras_clave":"GASTROSCHISIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64666,"idioma_id":5,"titulo":"Herramientas para aprender Física, Química y Tecnología","resumen":"Enel marco del Programa Conectar Igualdad consideramos necesario que los docentesse familiaricen con los programas disponibles en las netbooks y los utilicenpara favorecer el aprendizaje de Física, Química y Tecnología. Este artículopresenta las acciones realizadas en un taller para docentes de nivel secundarioa fin de introducirlos en el uso del programa CMapTools, como complemento de la lectura de textos disciplinares, aplicándolocomo recurso didáctico en el marco del escenario 1:1.","palabras_clave":"RECURSOS TICS|FÍSICA|QUÍMICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64667,"idioma_id":5,"titulo":"La resolución de problemas en la formación del profesorado en Ciencias: análisis de las opiniones y estrategias de los estudiantes","resumen":"En la formación inicial de docentes en Ciencias confluyen aspectos que se relacionan con la disciplina específica y con la formación didáctica. La resolución de problemas conjugaría ambos aspectos, constituyendo un recurso didáctico que influye en el proceso de formación inicial y en el futuro desempeño docente. El objetivo de este estudio es conocer las opiniones y las estrategias que mencionan los estudiantes, futuros docentes de Ciencias, en relación con la resolución de problemas. Para favorecer la explicitación de las ideas de los estudiantes utilizamos una técnica de frases incompletas partiendo del tronco verbal:&nbsp; «Cuando resuelvo problemas intento?». La muestra está constituida por estudiantes de los&nbsp; primeros años de los profesorados en Física y en Química de la UNSJ (Argentina). Las respuestas se vinculan especialmente con las habilidades cognitivas implicadas en la tarea,con la necesidad de consultar diferentes fuentes y se refieren en todos los casos a problemas cerrados en cuya resolución priorizan la etapa de identificación de datos. De los resultados obtenidos, referidos al concepto de problema que se infiere y a las estrategias que utilizan en su resolución, surgen cuestionamientos sobre la forma en que se está abordando la resolución de problemas en la formación inicial docente","palabras_clave":"RESOLUCIÓN DE PROBLEMAS|ESTUDIANTES|FORMACIÓN INICIAL DOCENTE|CIENCIAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64668,"idioma_id":5,"titulo":"Relaciones entre las representaciones de los alumnos acerca de la enseñanza y el aprendizaje de la Física y de la Química y la práctica docente","resumen":"En este trabajo  presentamos los resultados de una investigación realizada con alumnos y  docentes de educación secundaria de una escuela de la provincia de San Juan  (Argentina). El estudio realizado se enmarca en la teoría de las  representaciones sociales y tiene por objetivo identificar algunos aspectos que  pudieran influenciar el aprendizaje de la Física y de la Química. Durante esta  experiencia realizamos diferentes actividades: observaciones de clases, entrevistas  y encuestas, encuentros de reflexión, entre otras. Los resultados permiten  entender mejor la disposición de los alumnos hacia el aprendizaje de la Física  y de la Química y su desempeño en las mismas. Detectamos que los alumnos  manifiestan una actitud más positiva hacia el aprendizaje de la Química por  resultarles más fácil, si bien reconocen que, aunque el aprendizaje de la  Física les resulta difícil, contribuye al desarrollo de sus capacidades  intelectuales. Además, de esta experiencia inferimos una estrecha relación de  las representaciones que los alumnos construyen y expresan acerca de enseñar y aprender Física y Química con lo que  el profesor dice y hace en su práctica docente.","palabras_clave":"REPRESENTACIONES SOCIALES|ENSEÑANZAY Y APRENDIZAJE|FÍSICA Y QUÍMICA|PRÁCTICA DOCENTE|EDUCACIÓN SECUNDARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64669,"idioma_id":5,"titulo":"Representaciones sociales y práctica docente: una experiencia con profesores de Física y de Química","resumen":"En este artículo presentamos algunos de los resultados obtenidos en  una investigación que se desarrolla durante dos meses en un curso de nivel  secundario de una escuela urbana de la provincia de San Juan (Argentina), en  los espacios curriculares de Física y de Química. Este estudio se llevó a cabo  con el objetivo de detectar y analizar la vinculación entre las  representaciones sociales de los profesores y su práctica en el aula. Para esto  realizamos diferentes actividades tales como, observaciones de las clases,  entrevistas y encuestas a docentes y alumnos, encuentros de reflexión sobre  algunos resultados preliminares, entre otras. El análisis de los resultados  obtenidos sugiere una interrelación entre las representaciones sociales de los  profesores y su práctica docente. Así, según la representación de cada profesor  su práctica puede favorecer u obstaculizar el aprendizaje de contenidos científicos  y el interés de los alumnos por las ciencias. Por tal motivo, consideramos que  es necesario que los docentes puedan reflexionar sobre su práctica en el aula a  partir de la confrontación con sus representaciones sociales y con la  incidencia que éstas tienen en su quehacer, en los intereses y en el desempeño  de sus estudiantes.","palabras_clave":"REPRESENTACIONES SOCIALES|PRÁCTICA PEDAGÓGICA|FÍSICA|QUÍMICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64670,"idioma_id":5,"titulo":"Las evaluaciones en Ciencias Naturales: ¿qué aprendizaje se favorece desde la enseñanza en la educación secundaria?","resumen":"La enseñanza y  el aprendizaje de las Ciencias Naturales presentan en la actualidad dificultades  que se evidencian, entre otras cosas, en los bajos resultados de los  estudiantes en las evaluaciones. A fin de profundizar en esta problemática para  desde allí proponer acciones que permitan contribuir con la calidad del sistema  educativo, llevamos adelante una experiencia en la que estudiamos los procesos  de enseñanza y de aprendizaje en Física y en Química. La experiencia se  desarrolló con alumnos de nivel secundario y con docentes de Física y de  Química. Consideramos que la evaluación forma parte de los procesos de  enseñanza y de aprendizaje y es un elemento clave de la dimensión curricular.  Por esto analizamos las evaluaciones aplicadas a los alumnos y, en ellas, las  vinculaciones que se establecen entre los procesos de enseñanza y de aprendizaje.  Tanto las estrategias implementadas como el proceso de evaluación que los  docentes realizan nos permiten inferir una postura sobre qué es aprender  Ciencias. Los resultados obtenidos nos muestran que los alumnos tienen mejor  desempeño cuando lo que se pretende alcanzar es un aprendizaje memorístico, lo  que puede relacionarse con el bajo rendimiento en tareas que demanden capacidades  cognitivas más complejas. Favorecer sólo un aprendizaje memorístico puede  generar representaciones erróneas acerca de cómo construir el conocimiento  científico y, además, no contribuir al desarrollo de habilidades necesarias  para lograr un aprendizaje significativo y autónomo.","palabras_clave":"EVALUACIÓN|ENSEÑANZA SECUNDARIA|PRÁCTICA PEDAGÓGICA|APRENDIZAJE|FÍSICA|QUÍMICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64671,"idioma_id":5,"titulo":"Dificultades estratégicas en la comprensión lectora de estudiantes de Ciencias Naturales","resumen":"En esta investigación hemos estudiado, en alumnos y alumnas de nivel secundario, la comprensión lectora de un texto sobre el calor y su propagación. Diseñamos una prueba que incluye dos tareas en las que los estudiantes debieron: elaborar un organizador gráfico y redactar la idea principal. En la primera tarea evaluamos cómo el lector representa las ideas del texto y las conexiones que realiza en relación con la superestructura textual. Mediante la idea principal indagamos si logra identificar la macroestructura. Los resultados indican que a partir de la lectura del texto utilizado las dificultades trascenderían los ámbitos léxico, de estructura interna y de superestructura textual para situarse en un plano estratégico donde las principales dificultades son seleccionar, relacionar, reformular y resumir para obtener una producción coherente.","palabras_clave":"COMPRENSIÓN DE TEXTOS|LIBROS DE TEXTOS|DIAGRAMA|ANÁLISIS DE TEXTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64672,"idioma_id":5,"titulo":"En los márgenes de los márgenes. Procesos de individuación y experiencias migratorias de jóvenes en barrios populares del Área Metropolitana de Buenos Aires","resumen":"En este trabajo presentamos resultados de dos proyectos de investigación financiados, culminados recientemente, y un estudio doctoral en proceso, en el marco de los cuales analizamos los procesos de individuación en jóvenes en diversos barrios vulnerabilizados del Área Metropolitana de Buenos Aires. Para la construcción de datos empíricos realizamos entrevistas en profundidad y relatos biográficos, para cuyo análisis seguimos los lineamientos generales de la teoría fundamentada, utilizando como auxiliar el software Atlas.ti. Aquí nos centramos en el estudio de las experiencias migratorias de cinco jóvenes, de entre 16 y 23 años, provenientes de Paraguay. En primer lugar, presentamos algunas herramientas conceptuales utilizadas para el despliegue del problema. Luego abordamos las tensiones presentes en dichas experiencias entre los proyectos individuales, los vínculos afectivos y las condiciones estructurales de los países de origen y destino. En tercer lugar, analizamos las vivencias y las tácticas de los jóvenes frente a situaciones de discriminación y estigmatización. Finalmente, articulamos las categorías y proposiciones presentadas reflexionando sobre los posibles aportes de la sociología de la individuación y el enfoque biográfico para la visibilización y abordaje ?desde la investigación social y las políticas públicas? de los procesos de vulnerabilidad de jóvenes migrantes en barrios populares.","palabras_clave":"PROCESOS DE INDIVIDUACIÓN|JUVENTUDES|MIGRACIONES|BIOGRAFÍAS|VULNERABILIDADES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64673,"idioma_id":5,"titulo":"?Prevención del consumo problemático de drogas en la escuela: estrategia de formación docente en Argentina utilizando TIC?","resumen":"Desde 2010 nuestro equipo diseña  y coordina un curso virtual sobre Prevención del Consumo  Problemático de Drogas dictado desde el Ministerio de  Educación de la Nación Argentina. Al momento cuenta con tres ediciones en las que  participaron alrededor de 6000 docentes de todo el país. En el presente artículo  buscamos: a) analizar los discursos de los docentes participantes para identificar  sus posiciones en referencia al consumo de drogas; y b) reflexionar sobre las implicancias  de estas posiciones discursivas para el desarrollo en la escuela de estrategias  de promoción de la salud. Para el análisis del corpus seguimos los lineamientos  de la teoría fundamentada, identificando dos grandes posiciones discursivas, a las  que denominamos: modelo negativizante-unidimensional y modelo reflexivo-multidimensional.  Mientras que el primero conduce a la no implicación de los docentes y la institución  educativa en el abordaje de estos temas, el segundo propicia la generación de espacios  escolares de diálogo y de reconocimiento entre adultos y jóvenes.","palabras_clave":"ESCUELA|DOCENTES|JOVENES|CAPACITACION VIRTUAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64674,"idioma_id":5,"titulo":"Circulación de leptospiras patógenas en áreas periurbanas y rurales  del centro de la provincia de Buenos Aires, Argentina.","resumen":"Con el objetivo de identificar portadores de L. interrogans en Argentina, seatraparon animales silvestres en la provincia de Buenos Aires, capturando 95 Rattus norvegicus, 45 Didelphisalbiventris (comadrejas), 25 Chaetophractus villosus (armadillos), 12 Lycalopex griseus(zorro gris), 7 Conepatus chinga (zorrinos), 3 Galactis cuja (hurones), 3 Myocastor coypus (nutrias),3 Felis geoffroyi (gato montés), 1 felis spp. (gato salvaje) y 1 Dasypus septemcinctus (mulitas).Todos fueron testeados mediante el test de microaglutinación,y 47 (25,8%) fueron positivos paraLeptospira Ballum, Canicola, Grippotyphosa,Icterohaemorrhagiae, Hardjo, Hebdomadis, Pomona y Pyrogenes a títulos de1:50 y 1:200. Tejido renal de 133 animales fueron cultivados y 28 aislamientosde L. interrogans fueronobtenidos a partir de R. norvegicus (25\/28), de L. griseus(1\/28), de C. chinga(1\/28) y de D. albiventris (1\/28).Los hámsteres inoculados con los aislamientos murieron entre los 4-6 días,observándose lesiones macro y microscópicas en pulmones, riñón e hígado. Através de múltiple-locus variable-number tandem repeat analysis (MLVA), 9aislamientos presentaron un perfil genético similar a L. interrogans Icterohaemorrhagiae RGA, 12 a L. interrogansIcterohaemorrhagiae Copenhageni, yuna&nbsp; a L. interrogans Canicola HondUtrecht IV.","palabras_clave":"LEPTOSPIRA INTERROGANS ICTEROHAEMORRAGIAE|LEPTOSPIRA INTERROGANS  CANICOLA|MULTIPLE LOCUS VARIABLE NUMBER TANDEM ANALYSIS|LEPTOSPIROSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64675,"idioma_id":4,"titulo":"Electrogenerated Conductive Polymers from Triphenylamine End- Capped Dendrimers","resumen":"Electroactive end-capped dendritic macromolecules were designed and synthesized. Their structures contain triphenyl-amine moieties as part of the core or dendrons. The electro-generated &#64257;lms produced with these monomers behaved as conductive dendritic polymers that can be reversible charged, both in the core and in the peripheral units. The design of dendrimer structures with the introduction of systematic changes allows to establish relationships between their electro-optical properties with  molecular structural parameters. The &#64257;lms of polymeric material hold good electrical conductivity, reversible electrochemical processes and chemical stability. The results indicate that the use of conjugated and rigid structures in dendritic macromolecules is an important factor in order to obtain electropolymeric &#64257;lms. This work provides a model to design starburst dendrimers capable to form electrochemically active polymers with potential applications in electronic and optoelectronic devices.","palabras_clave":"DENDRITIC POLYMERS|ELECTRODEPOSITION|OPTOELECTRONIC"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64676,"idioma_id":5,"titulo":"Isotermas de adsorción de agua en champiñones","resumen":"Se han estudiado las  isotermas de adsorción de agua en champiñones en el rango de 30 a 70°C. Se ha  utilizado para ello el método estático usando soluciones salinas saturadas para  proveer actividades da agua definidas. Se han utilizado nueve ecuaciones  diferentes para el ajuste de los datos experimentales, utilizándose el coeficiente de regresión como criterio de bondad del  ajuste. Las ecuaciones biparamétricas de  Bradley, Chung y Pfost, y Henderson&nbsp; son  las que permiten los mejores ajustes. Se ha determinado el&nbsp; calor isostérico neto de adsorción utilizando  la ecuación de Clausius-Clayperon. Se observa un incremento significativo de  este calor al disminuir la humedad del champiñon.","palabras_clave":"isotermas |adsorción |humedad|champiñones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64677,"idioma_id":5,"titulo":"Determinación de reducción del crecimiento radical (CE50) por una formulación de glifosato utilizando lechuga y trigo como especies bioindicadoras","resumen":"    El objetivo fue  determinar la concentración efectiva media de reducción del crecimiento radical  (CE50), de una formulación del herbicida glifosato mediante bioensayos de  germinación con semillas de lechuga y de trigo. Para lechuga se probaron 9  dosis\/tratamientos decrecientes entre 1.215 y 0,01215 g i.a.ha-1  (formulado: Sal amónica de la   N-Fosfonometil glicina, 40,5 [g e.a. glifosato 36,9% p\/v]) y  para trigo 5 dosis decrecientes (entre 12,15 y 1,215 g i.a.ha-1) y su  respectivo control negativo (agua destilada). Se utilizaron cajas de Petri, con  papel de filtro en la base humedecido con 3 ml de la solución correspondiente.  Se sembraron 20 semillas por caja, distribuyéndose los tratamientos en bloques  al azar con 4 repeticiones, en cámara de crecimiento a 20°C con alternancia de luz y  oscuridad. Se midió la longitud radicular de todas las semillas de cada  tratamiento con calibre digital. Se determinó el porcentaje de germinación y se  calculó el índice de germinación. Las CE50, es decir, las dosis que redujeron  en un 50% el crecimiento radical para lechuga y trigo, fueron 6,682 y 9,416 g i.a.ha-1,  respectivamente. Los materiales probados resultaron sensibles a distintas dosis  de glifosato y por lo tanto pueden utilizarse como indicadores biológicos de  toxicidad específica.   ","palabras_clave":"GERMINACION|TOXICIDAD|PRUEBAS BIOLOGICAS|GLIFOSATO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64678,"idioma_id":5,"titulo":"CARACTERÍSTICAS DE PERSONALIDAD EN EX COMBATIENTES Y VETERANOS DE LA GUERRA DE MALVINAS","resumen":"El  trabajo analiza las características de personalidad de excombatientes y  veteranos de la guerra de Malvinas con el fin de obtener datos de utilidad para  la intervención terapéutica. Se analizaron los resultados del MMPI-2 (adaptación  argentina, Casullo, 1999) correspondientes a 44 veteranos y 96 ex combatientes consultantes  del Centro Salud de las Fuerzas Armadas ?Veteranos de  Malvinas?, dependiente del Ministerio de Defensa. Los resultados indican que&nbsp; ambos  grupos tienden a la exageración de malestar, refiriendo fuertes sentimientos de  ansiedad, sensaciones de descontrol ego-cognitivo y emocional, problemas  interpersonales, síntomas de depresión y una fuerte orientación paranoide. Presentan  perfiles comunes con la clínica, señalando dificultades severas en el ajuste  psicológico. Las diferencias entre grupos indican que los excombatientes  tienden a expresar más abiertamente &nbsp;que  los veteranos&nbsp; los sentimientos de  tensión, evidenciando también mayor propensión a los conflictos con la  autoridad y con el entorno. ","palabras_clave":"PERSONALIDAD|VETERANOS|MALVINAS|MMPI-2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64679,"idioma_id":5,"titulo":"Estudio epidemiológico de los casos de triquinelosis registrados en la provincia de Santa Fe, Argentina, 1998?2009","resumen":"Objetivo. Caracterizar desde los puntos de vista epidemiológico, clínico y de laboratorio  los casos y brotes de triquinelosis que se registraron en la provincia de Santa Fe, Argentina,  entre 1998 y 2009.  Métodos. Se analizaron 1 519 fichas epidemiológicas de personas que presentaron síntomas  y signos compatibles con triquinelosis en la provincia de Santa Fe en el período 1998\u20132009.  Se realizó el análisis estadístico descriptivo de la información contenida en las fichas  epidemiológicas y los resultados clínicos y de laboratorio; se aplicó el análisis bifactorial para  determinar las posibles asociaciones con factores epidemiológicos y ambientales.  Resultados. Se encontraron síntomas de triquinelosis en 1 276 casos; 372 cumplían la  definición de caso clínico y 224 muestras resultaron positivas por inmunofluorescencia  indirecta, independientemente de su clasificación como caso clínico. Se identificaron 27 brotes  que involucraron a 1 157 casos en ocho departamentos, con mayor frecuencia en el centro y el  sur de la provincia. La distribución anual de los casos asociados epidemiológicamente entre sí  fue heterogénea, con una mayor frecuencia en el período 2000\u20132003 y en los meses de agosto  a octubre. Hubo mayor probabilidad de enfermar cuando se consumieron productos de fuentes  informales (OR = 3,69; P = 0,014) y en las áreas rurales (OR = 1,799; P = 0,011). El período de  incubación de la enfermedad (mediana) fue de 12 días. Las personas que consumieron carne de  cerdo o sus derivados tuvieron 2,06 veces más riesgo de enfermar que las que no la consumieron.  Conclusiones. El mayor número de brotes ocurrió en los departamentos más poblados y con  mayor producción porcina. Se deben diseñar acciones específicas de prevención y control de la  enfermedad en esos departamentos. Se debe considerar el análisis epidemiológico de riesgos al  evaluar, gestionar y comunicar los riesgos sanitarios de triquinelosis.","palabras_clave":"Triquinelosis|Porcinos|Epidemiologia|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64680,"idioma_id":4,"titulo":"Occurrence of thermotolerant Campylobacter spp. at different stages of the poultry meat supply chain in Argentina","resumen":"AIMS: The objectives of this study were to investigate the occurrence and concentration of thermotolerant Campylobacter spp. at different stages of the poultrymeat supply chain in Argentina. METHODS: Three integrated poultry companies were sampled. Each supply chain was considered at different stages from the reproductive farm to chicken meat at a retail market. The stages sampled were: (a) hens from breeder flocks, (b) eggs in the incubator, (c) broiler chickens in flocks (aged 5 weeks), (d) chickens at a slaughterhouse, and (e) chicken meat at a retail market. The chickens sampled along each supply chain were in the same batch. Samples collected were: (a) cloacal samples from hens and chickens on the farms, (b) fertile eggs, (c) feed, water and litter from flocks, (d) chicken carcasses from the slaughterhouse and retail market, and (e) caeca and livers from the slaughterhouse. Samples obtained were examined for Campylobacter spp. The isolates were biotyped and the genus and species identified by PCR. Campylobacter spp. on chicken carcasses at slaughterhouse and retail market were enumerated. RESULTS: The highest proportions of Campylobacter positive samples were observed in carcasses at retail (25\/30, 83.3%) and faecal samples from breeding hens (27\/45, 60.0%). Only 3.3% (3\/90) samples collected from broiler chickens aged <1 week were positive, but the percentage of positive samples had risen to 28.9% (26\/90) by the end of the rearing period. The proportions of Campylobacter positive carcasses and caecal contents at the slaughterhouse were both 33.3% (10 of 30 samples each). The concentration of Campylobacter contamination observed on carcasses at retail markets ranged from no bacteria\/carcass to 3.71 log10 cfu\/carcass.","palabras_clave":"Campylobacter spp.|Chickens|Zoonosis|farm-to-fork"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64681,"idioma_id":4,"titulo":"Effect of electrolyte concentration on the electrochemical properties of an AB5 .type alloy for Ni\/MH batteries","resumen":"This publication is the first of a series of three that we have undertaken to study the effect of electrolyte concentration on electrode performance. Here, the electrochemical properties of an AB5-type alloy, namely LaNi3.6Co0.7Mn0.4Al0.3, are investigated using different KOH&nbsp; electrolyte concentrations (i.e. 2 M, 4 M, 6 M and 8 M). The next two publications will be concerned with an AB2-type alloy and a Mg-based alloy, respectively. From the present study, the 6 M and 8 M electrodes were those that exhibited faster activation, maximum discharge capacity values (w325 mAh\/g) and best performance under high-rate dischargeability. Nevertheless, the capacity loss rate increased as KOH concentration augmented.The electrochemical behavior is analyzed in terms of a physicochemical model based on the theory of porous electrodes. The fitting of electrochemical impedance spectroscopy&nbsp; data in terms of the model allowed determining kinetic parameters of the hydriding reaction, transport of hydrogen in the alloys and structural electrode parameters.","palabras_clave":"BATTERY|ALLOY|HYDRIDE|ENERGY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64682,"idioma_id":4,"titulo":"The perception of Chemistry of first-year undergraduate students at the University of Buenos Aires","resumen":"At the University of Buenos Aires, Argentina, the first undergraduate Chemistry course is taught within the Ciclo Básico Común (CBC), aimed at students of Technology, Health and Basic Sciences. Its massive attendance (30000 students per year) gives ? along with the development of  fundamental topics of the discipline? the chance to discuss the importance of Chemistry for the Society as a whole to a broad spectrum of  teenagers, coming from many different backgrounds. Moreover, it shows the relevance of these topics in relation to the different careers chosen by the students. In this work, we analyzed how a group of young students perceive the Chemical Sciences by means of instruments such as questionnaires, interviews and workshops. We gathered around  600 opinions. Our results suggest that Chemistry is perceived by these students neither as a ?polluting? nor a ?dangerous? discipline, but rather as a body of knowledge with both vague and diffuse interests.","palabras_clave":"chemistry|perception|introductory course|undergraduate students"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64683,"idioma_id":4,"titulo":"On a possible origin for the lack of old star clusters in the Small Magellanic Cloud","resumen":"We model the dynamical interaction between the Small and LargeMagellanic Clouds and their corresponding stellar cluster populations. Our goal is to explore whether the lack of old clusters (>7 Gyr) in the Small Magellanic Cloud (SMC) can be the result of the capture of clusters  by the Large Magellanic Cloud (LMC) as well as their ejection due to the tidal interaction between the two galaxies. For this purpose, we perform a suite of numerical simulations probing a wide range of parameters for the orbit of the SMC about the LMC. We find that, for orbital eccentricities e &#8805; 0.4, approximately 15 per cent of the SMC clusters are captured by the LMC. In addition, another 20?50 per cent of its clusters are ejected into the intergalactic medium. In general, the clusters lost by the SMC are the less tightly bound cluster population. The final LMC cluster distribution shows a spatial segregation between clusters that originally belonged to the LMC and those that were captured from the SMC. Clusters that originally belonged to the SMC are more likely to be found in the outskirts of the LMC. Within this scenario, it is possible to interpret the difference observed between the star field and cluster  SMC age?metallicity relationships for ages 7 Gyr.","palabras_clave":"methods: numerical|galaxies: individual: SMC|Magellanic Clouds|galaxies: star clusters: general"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64684,"idioma_id":5,"titulo":"UNA ALTERNATIVA DE PRODUCCION EN VIVIENDA: EL SISTEMA BENUMA","resumen":"Desde su visión institucional y   entendiendo a la cuestión habitacional   como una problemática compleja, es   decir como un hecho no sólo espacial   sino también económico, social,   político-institucional y cultural, AVE-  CEVE sostienen que junto a la creación   y transferencia de tecnologías   constructivas y de gestión apropiadas   y apropiables, orientadas al desarrollo   integral de los sectores de bajos   recursos, se adiciona la necesidad   imperante de que las tecnologías   transferidas tiendan a  fortalecer las   estructuras institucionales y sistemas   productivos locales (micro y pequeños   emprendimientos, cooperativas de   trabajo, asociaciones de usuarios,   etc.) contribuyendo en alguna medida   a la generación de empleo en   aquellos sectores más postergados en   situaciones de crisis.  La concepción de tecnología   constructiva de AVE-CEVE supone que   la misma necesariamente se transforme   en un  método de construir , y por lo   tanto, una forma de producir el hábitat   popular, una forma de generar trabajo,   una manera de satisfacer necesidades   y por ende una forma diferente de   concebir a nuestras sociedades y de   procurar otro tipo de desarrollo, más   humano y equitativo.  Al incorporar el concepto de   integralidad a los procesos de   producción del hábitat, también   se considera que este método   para construir deberá  favorecer la   organización de grupos sociales. Por   lo cual, la tecnología debe ser flexible   y permitir la participación efectiva de   la población en el proceso de gestión   habitacional como en el de ejecución,   y también  favorecer la incorporación   de mano de obra no calificada y el uso   de materiales tradicionales disponibles   en el mercado.   También se intenta que los sistemas   constructivos desarrollados, permitan   en la ejecución de las viviendas, la   incorporación de una multiplicidad de   formas productivas entre las que se   pueden mencionar el auto consumo,   la ayuda mutua, los planes de estado,   la ayuda mutua rentada y las micro y   pequeñas empresas de la construcción;   que son características del sector social   destinatario de la institución.  Bajo este enfoque, se concibe el   sistema constructivo BENUMA, cuyo   nombre deriva de la combinación   de dos sistemas constructivos ya   patentados por CEVE hace muchos   años: el UMA (Patente Nº322688\/92)","palabras_clave":"Hábitat|Vivienda económica|Sistemas constructivos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64685,"idioma_id":4,"titulo":"Fingerprint Database Variations for WiFi Positioning","resumen":"Indoor positioning systems calculate the position of a mobile device (MD) in an enclosed environment with relative precision. Most systems use WiFi infrastructure and several positioning techniques, where the most commonly used parameter is RSSI (Received Signal Strength Indicator). In this paper, we analyze the fingerprinting technique to calculate the error window obtained with the Euclidian distance as main metric. Build variations are presented for the Fingerprint database analyzing various statistical values to compare the precision achieved with different indicators.","palabras_clave":"INDOOR POSITIONING|RSSI|FINGERPRINT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64686,"idioma_id":4,"titulo":"Indoor Positioning Using the Modified Fingerprint Technique","resumen":"The Wi-Fi positioning systems available for enclosed spaces use the existing network infrastructure to calculate the position of the mobile device (MD). The most commonly used parameter is RSSI (Received Signal   Strength Indicator). In this paper, we analyze the Fingerprint technique considering some variations aimed at improving the accuracy of the technique and minimizing calculation time. Significant field work is carried out, analyzing the accuracy achieved with each technique.","palabras_clave":"INDOOR POSITIONING|WIRELESS LAN|RSSI|FINGERPRINT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64687,"idioma_id":4,"titulo":"WiFiPos: An In\/Out-Door Positioning Tool","resumen":"Geographical location solutions have a wide diversity of applications, ranging from emergency services to access to tourist and entertainment services. GPS (Global Positioning System) is the most widely used system for outdoor areas. Since it requires a direct line of sight with the satellites, it cannot be used indoors. For indoor positioning, the most commonly used technology to calculate the position of mobile devices is Wi-Fi. In this article, we present a positioning tool called WiFiPos based on Wi-Fi signal processing. This tool uses different variations of the Fingerprint technique to analyze the performance and accuracy of the system both indoors and outdoors.","palabras_clave":"INDOOR POSITIONING|OUTDOOR POSITIONING|RSSI|FINGERPRINT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64688,"idioma_id":4,"titulo":"Phenotypic flexibility in response to environmental salinity in the euryhaline  crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon","resumen":"This study constitutes a first attempt to investigate  intraspecific differences in osmoregulatory capacity  and digestive and metabolic responses at the biochemical  level in relation to hyper- and hypo-regulation in a single  species of estuarine crab inhabiting contrasting habitats  within a same intertidal area. We compared hemolymph  osmolality, key digestive enzymes, glycemia and energy  reserves in Neohelice granulata (Dana in Proc Acad Nat  Sci Philadelphia 5:247?254, 1851) from the mudflat and  saltmarsh of Mar Chiquita coastal lagoon (37320\/37450S-  57190\/57260W) under a wide range of salinities  (6?60 psu). Individuals from both sites exhibited high and  similar osmoregulatory capacity, but while in individuals  from mudflat low and high salinities affected lipase activity  in hepatopancreas and triglycerides in muscle, in crabs  from saltmarsh, high salinities affected glycogen in anterior  gills. Low salinity differentially affected free glucose in  anterior gills. The results suggest the occurrence of intraspecific  distinct digestive and metabolic adjustments in  relation to osmoregulatory responses and habitat.","palabras_clave":"CRABS|NEOHELICE GRANULATA|OSMORREGULATION|DIGESTIVE ENZYMES|RESERVES|Phenotypic flexibility"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64689,"idioma_id":4,"titulo":"Study and determination of As, Cr and Pb in amaranth seeds","resumen":"The concentration of As, Cr and Pb toxic elements was determined in three species of amaranth seeds: A. hypochondriacus, A. cruentus and A. dubius. The determinations were carried out by inductively coupled plasma optic atomic spectroscopy (ICP-OES). The result shows that Cr and As were found in minor concentrations than allowed by the World Health Organization (WHO; 120 and 4 mg kg-1 respectively); As (mg kg-1): A. dubius (0.76) A. cruentus (<0.50) and A. hypochondriacus (< 0.50); Cr (mg kg-1):  A. dubius (9.79) A. cruentus (1.55) and A. hypochondriacus (1.52). However, the concentration of Pb was high (A. dubius (19.04) A. cruentus (30.20) and A. hypochondriacus (35.56) more than the maximum of WHO (10 mg kg-1). To avoid systematic error, recovery and validation studies were performed: recovery test: 102.3%; validation (by standard addition): 96.0 \u2013 103.1%. Due to the fact that amaranth had been proposed as new food due to its nutraceutical properties, the high concentration of Pb found in this study indicates that Pb should be evaluated in these amaranth species to avoid the intake of toxic element by human beings.","palabras_clave":"FOOD |TOXIC|ELEMENTS|AMARANTH|SEEDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64690,"idioma_id":5,"titulo":"Guidelines for diagnosis, monitoring and treatment of Fabry disease","resumen":"La enfermedad de Fabry es un trastorno de almacenamiento lisosomal hereditario ligado al cromosoma X, ocasionado por el déficit de la enzima alfa galactosidasa A. El conocimiento sobre estapatología, y en particular su manejo médico, ha progresado notablemente en la última década, incluyendo eldesarrollo de su tratamiento específico. La presente guía fue desarrollada por profesionales médicos de diversas especialidades involucrados en la atención de pacientes con enfermedad de Fabry. La discusión y análisis de las evidencias científicas disponibles, sumado a la experiencia de cada uno de los participantes, ha permitido desarrollar los conceptos vertidos en esta guía con el objetivo de brindar una herramienta útil para todos los profesionales que asisten a pacientes con enfermedad de Fabry.","palabras_clave":"ENFERMEDAD DE FABRY|GUIA|DIAGNOSTICO|SEGUIMIENTO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64691,"idioma_id":4,"titulo":"Predatory insects in lentic freshwater habitats from Northwest Patagonia: richness and phenology","resumen":"The main purpose of this work was to study the richness, phenology and abundance of predatory insects throughout the hydroperiod and how they relate to the environmental parameters in lentic bodies of water in northwest Patagonia. Nineteen fishless wetlands and three wetlands with fish were studied, which are located in the surroundings of Nahuel Huapi National Park. Biotic and abiotic variables were measured during the hydroperiod of each wetland. The dip-netting sampling technique was used to determine the richness of predatory insects and to study the phenology of the dominant species in four wetlands. Species richness in temporary wetlands ranged from 1 to 9 species per wetland and does not differ from that observed in the permanent environments with or without fish predators. Maximum insect richness was recorded in late spring and was associated to the maximum depth of the wetland and its structural complexity as well as to the duration of the hydroperiod.","palabras_clave":"aquatic insects|richness|hydroperiod wetland|phenology|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64692,"idioma_id":4,"titulo":"Do protostellar fountains shape the regional core mass function?","resumen":"The emerging massive binary system associated  with AFGL 961 signifies the latest generation of massive star and cluster  formation in the Rosette Molecular Complex. We present the detection of a  compact cluster of dusty cores toward the AFGL 961 region based on  continuumimaging at 1.3mm by the Submillimeter Array. The binary components of  AFGL 961 are associated with the most intensive millimeter emission cores or  envelopes, confirming that they are indeed in an early stage of evolution. The  other massive cores, however, are found to congregate in the close vicinity of  the central high-mass protostellar binary. They have no apparent infrared counterparts  and are, in particular, well aligned transverse to the bipolar molecular outflows  originating from AFGL 961. This provides evidence for a likely triggered origin  of the massive cores. All 40 individual cores with masses ranging between 0.6 and  15M¡Ñ were detected above a 3 _ level of 3.6 mJy beam&#8722;1 (or 0.4M¡Ñ), based on which  we derive a total core mass of 107 M¡Ñ in the AFGL 961 region. As  compared to the stellar initial mass function, a shallow slope of 1.8 is,  however, derived from the best fit to the mass spectrum of the millimeter cores  with a prestellar and\/or protostellar origin. The flatter core mass  distribution in the AFGL 961 region is attributed here to dynamic perturbations  from the massive molecular outflows that originated from the massive  protostellar binary, which may have altered the otherwise more quiescent conditions  of core or star formation, enhanced the formation of more massive cores and, as  a result, influenced the core mass distribution in its close vicinity.","palabras_clave":"star formation|matter|mass function"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64693,"idioma_id":5,"titulo":"El tercer gobierno de Perón y la fractura de la clase dominante","resumen":"En los años que transcurrieron entre el golpe de estado de 1955 y el retorno de Perón en 1973, la Argentina atravesó una de sus más profundas crisis. La acumulación de capital se encontraba estancada, la clase dominante dividida y ninguno de los muchos gobiernos (y regímenes políticos) que se sucedieron consiguió revertir la situación. A su vez, en 1969, se inició un proceso de movilización social y radicalización política que puso en peligro el sistema en su conjunto. Uno de los aspectos menos estudiados de este período es la intervención política de la clase dominante. En este artículo nos proponemos avanzar sobre este problema, abordando las estrategias de las diferentes capas de la burguesía industrial y agropecuaria frente a la apertura democrática y el retorno de Perón. Tomaremos como observables a las corporaciones empresarias, sus posicionamientos, sus alianzas y sus disputas internas.","palabras_clave":"PERONISMO|TRANSICIÓN DEMOCRÁTICA|BURGUESÍA INDUSTRIAL|BURGUESÍA AGRARIA|CORPORACIONES EMPRESARIAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64694,"idioma_id":5,"titulo":"Corporaciones empresarias, alianzas sociales y disputas políticas en el retorno del peronismo (1969-1974)","resumen":"El período de la historia argentina que se extiende entre el Cordobazo y el retorno de Perón ha sido particularmente turbulento. A la crisis de acumulación que se arrastraba desde la década de 1950, se sumó una activación popular que derivó en una crisis de régimen y forzó la apertura democrática. En este terreno convulsionado se gestó el retorno de Perón, proscrito durante 18 años. Además de los apoyos que su retorno suscitó en la clase obrera y la pequeña burguesía, el Peronismo contó con el respaldo de distintos sectores de la clase dominante. En muchos casos, se trató de los mismos sectores que habían avalado el golpe de 1955 y la proscripción de los años subsiguientes. En este artículo nos proponemos analizar, a partir de la reconstrucción de la intervención política de las principales corporaciones empresarias, las disputas en el seno de la clase dominante en torno al retorno del Peronismo. Observaremos el resurgimiento de la alianza reformista, sus enfrentamientos con el polo 'liberal' y la atracción que despertó el Peronismo sobre distintos núcleos empresarios. Esperamos contribuir al conocimiento de uno de los aspectos menos estudiados de este proceso.","palabras_clave":"CORPORACIONES EMPRESARIAS|PERONISMO|BURGUESÍA AGRARIA|BURGUESÍA INDUSTRIAL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64695,"idioma_id":5,"titulo":"Modelo matemático y simulación de un motor de ciclo Stirling empleando el software Simusol","resumen":"El desarrollo e investigación de motores Stirling han despertado la atención de muchos científicos institutos y empresas comerciales. El motor Stirling es tanto práctica como teóricamente un dispositivo fiable y seguro, reconocido dos siglos después de su invención por Robert Stirling en 1816. Funciona en un ciclo termodinámico regenerativo cerrado y reversible. El objetivo de este documento es proporcionar información y presentar una revisión para el desarrollo de la máquina Stirling, el ciclo del motor y una técnica de simulación utilizada para el análisis y optimización del mismo. El modelo matemático empleado es el modelo adiabático de cinco volúmenes. La simulación se desarrolla empleando el software SIMUSOL, de libre circulación. Se obtienen las curvas del ciclo Stirling y las de temperatura en diferentes puntos de interés y para diferentes presiones de trabajo. La precisión global de la simulación es satisfactoria.","palabras_clave":"STIRLING|SIMULACIÓN|SIMUSOL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64696,"idioma_id":4,"titulo":"Intramolecular charge separation in spirobifluorene-based donor-acceptor compounds adsorbed on Au and Indium Tin Oxide electrodes","resumen":"Surface photovoltage (SPV) measurements were performed with a Kelvin-probe in spirobifluorene-based donor (diphenylamine)?acceptor (dicyano or cyanoacrylic acid moieties) compounds adsorbed from highly diluted solutions onto Au and indium tin oxide electrode surfaces. Strong intramolecular charge separation (negative SPV signals up to more than 0.1 V) due to directed molecule adsorption was observed only for spirobifluorene donor?acceptor compounds with carboxylic acid moiety. SPV signals and onset energies of electronic transitions depended on ambience conditions.","palabras_clave":"Surface photovoltage  |Spirobifluorene|Charge separation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64697,"idioma_id":4,"titulo":"Charge Separation in Donor-Acceptor Spiro Compounds at Metal and Metal Oxide Surfaces Investigated by Surface  Photovoltage","resumen":"Molecules with donor (diphenylamine) and acceptor moieties (dicyano or cyanoacrylic acid moieties) were linked by fluorene or spirobisfluorene cores and the chain length has been changed by introducing a thiophene group between fluorene and diphenylamine. Four different kinds of fluorene and spirobisfluorene compounds were adsorbed from highly diluted solutions at ultra-thin nanoporous TiO2 (np-TiO2), Au and ITO surfaces. Charge separation has been investigated by surface photovoltage  spectroscopy in the fixed capacitor and Kelvin probe arrangements in vacuum. Striking differences between the interaction of linking(dicyano or cyanoacrylic moieties) and different substrates were observed. Intra-molecular charge separation and electron injection have been distinguished and the directed adsorption of spiro compounds was deduced.","palabras_clave":"SPIRO|SURFACE PHOTOVOLTAGE|CHARGE SEPARATION|METAL SURFACE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64698,"idioma_id":4,"titulo":"Synthesis and Photovoltaic Applications of a 4,4 -Spirobi[cyclopenta-[2,1;b;3,4 b]dithiophene]-Bridged Donor\/Acceptor Dye","resumen":"A new donor\/acceptor (D A) spiro dye (SCPDT1) featuring two bithiophene units, connected through an sp3 -hybridized carbon atom, was prepared by a multistep synthetic sequence involving the convenient assembly of the spiro system under mild catalytic conditions. The photocurrent spectrum of dye-sensitized solar cells incorporating SCPDT1 covers the spectral region ranging from 350 to 700 nm and reaches a wide maximum of &#8764;80% in the 420 560 nm range. Power conversion efficiencies of up to 6.02% were obtained.","palabras_clave":"Solar Energy Conversion|Dye Solar Cells|Spirodithiophene"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64699,"idioma_id":4,"titulo":"Calculation of the Effective Diffusion Coefficients in Drying of Chemical and Mechanical Pretreated  Rosehip Fruits (Rosa eglanteria L.) with Selected Mass Transfer Models","resumen":"The aim of this work was to select models of mass transfer to estimate and compare effective mass diffusion coefficients (Deff) during the dehydration of rosehip (Rosa eglanteria) fruits with air at 70ºC. Fruits were pre-treated both chemically and mechanically, which include dipping the fruits in NaHO and ethyl oleate solutions; and cutting or perforating the fruit cuticle, respectively. Simple models, which minimize the time of calculation during the simulation of deep-bed driers, were adopted. Chosen models used to fit the drying data were the diffusive equation developed by Becker and the analytical solution of Fick´s second law. In both case, fruit shrinkage during drying was considered. Deff values were evaluated maximizing the multiple correlation coefficients (R2) and minimizing the mean percentage error (%). Results show that evaluated models satisfactorily predict the drying of rosehip fruit, and obtained (Deff) deferred less than 2 %. It was also found that the implementation of different types of pre-treatments on the skin of the fruits, to total or partially remove this waxen cuticle, noticeably improved water diffusion, reducing the time of processing. Mechanical pretreatment resulted the more effective as rosehip fruit drying speed-up method.","palabras_clave":"DIFFUSION COEFFICIENT|DRYING|ROSE HIP|MODEL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64700,"idioma_id":4,"titulo":"Role of hydrophobicity in adhesion of wild yeast isolated from ultrafiltration membranes of an apple juice processing plant","resumen":"The role of cell surface hydrophobicity in the adhesion to stainless steel of eleven wild yeast strains isolated from ultrafiltration membranes of an apple juice processing plant was investigated. The isolated yeast belonged to four species: Candida krusei (5 isolates), Candida tropicalis (2 isolates), Kluyveromyces marxianus (3 isolates) and Rhodotorula mucilaginosa (one isolate).Surface hydrophobicity was measured by Microbial Adhesion To Solvents (MATS) method. Yeast cells and surfaces were incubated in apple juice and temporal measurements of the numbers of adherent cells were made. Ten isolates showed moderate to high hydrophobicity and one strain was hydrophilic. &nbsp;The hydrophobicity expressed by the yeast surfaces correlated positively with the rate of adhesion of each strain. These results indicated that cell surface hydrophobicity governs the initial attachment of the studied yeast strains to stainless steel surfaces common to the apple juice processing plants.","palabras_clave":"ULTRAFILTRATION MEMBRANES|YEAST|APPLE JUICE|HYDROPHOBICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64701,"idioma_id":4,"titulo":"The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis","resumen":"Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm development. We found that the anr mutant was reduced in its ability to attach, to form aggregates and to display twitching motility but presented higher swimming motility than the wild type. In addition, microscopy revealed that the wild type biofilm contained more biomass and was thicker, but were less rough than that of the anr mutant. In silico analysis of the P. extremaustralis genome for Anr-like binding sites led to the identification of two biofilm-related genes as potential targets of this regulator. When measured using  Quantitative Real Time PCR, we found that the anr mutant expressed lower levels of  pilG , which encodes a component of Type IV pili and has been previously implicated in cellular adhesion. Levels of morA, involved in signal transduction and flagella development, were also lower in the mutant.  Our data suggest that under low oxygen conditions, such as those encountered in biofilms, Anr differentially regulates aggregation and motility thus affecting the first stages of biofilm formation.","palabras_clave":"BIOFILM|PSEUDOMONAS|ANR|CELL ATTACHMENT"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64702,"idioma_id":4,"titulo":"PAINTING RUSTED STEEL. THE ROLE OF ALUMINUM PHOSPHOSILICATE","resumen":"Surface preparation is a key factor for the adequate performance of a paint system. The aim of this investigation is to employ a wash-primer to accomplish the chemical conversion of rusted surface when current cleaning operations are difficult to carry out. The active component of the wash-primer was aluminum phosphosilicate whose electrochemical behavior and the composition of the generated protective layer, both, were studied by electrochemical techniques and scanning electron microscopy (SEM), respectively. Primed rusted steel panels were coated with an alkyd system to perform accelerated tests in the salt spray chamber and electrochemical impedance measurements (EIS). These tests were conducted in parallel with a chromate wash primer and the same alkyd system. Results showed that the wash-primer containing aluminum phosphosilicate could be used satisfactorily to paint rusted steel exhibiting a similar performance to the chromate primer.","palabras_clave":"ORGANIC COATINGS|STEEL|EIS|SEM"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64704,"idioma_id":4,"titulo":"EVALUATION OF PROTECTIVE DUPLEX SYSTEMS DEPOSITED ON STEEL AND EXPOSED TO NATURAL OR ARTIFICIAL ATMOSPHERIC CONDITIONS","resumen":"The corrosion  performance of steel\/55%Al-Zn alloy sheets pre-treated with 5% Fe (NO3)3  + 15% H3PO4 solution, and then coated with acrylic (AC),  alkyd (AQ), vinyl (VL), solvent-based epoxy (ES) or waterborne epoxy (EA)  corrosion-inhibiting primers + a barrier topcoat alkyd paint was studied.  Exposure conditions included weathering chambers and natural atmospheres (La Plata-Argentina  and San Luis-Potosí, México). The panels? behavior was evaluated through visual  inspections and EIS measurements. Their results allowed inferring that: 1) most  of the painting systems have a satisfactory performance during their exposure  to the accelerated weathering chamber or the natural atmospheric stations; and 2)  the correlation between them allowed explaining some problems arising in service.  This information will allow extending the useful life by adjusting the painting  system formulation.","palabras_clave":"STEEL\/55%Al-Zn|PAINTNG SYSTEMS|ADHESION|WEATHERING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64705,"idioma_id":4,"titulo":"Major lake level fluctuations and climate changes for the past 16,000 years as reflected by diatoms and chironomids preserved in the sediment of Laguna Potrok Aike, southern Patagonia","resumen":"Laguna Potrok Aike is a maar lake located in southernmost Argentina and is one of the few permanent lakes preserving a continuous climatic record from the semiarid Patagonian steppe. Furthermore, its location close to Antarctica provides a unique opportunity to compare paleoclimate from continental South America with the polar records. The analysis of subfossil chironomids and diatoms throughout a 16-m sedimentary record retrieved from this lake, combined with a well-constrained chronology of the last ca 16 ka BP, provided a high resolution paleoenvironmental reconstruction of the limnology of the lake and regional climate conditions. The combination of both bioproxies showed humid conditions  during the Lateglacial, followed by drier conditions during the Holocene, resulting in large variations in lake level. Despite not showing a clear evidence of a cold reversal similar to the Antarctic Cold Reversal  and\/or the Younger Dryas, both records suggest high water levels and oligotrophic conditions between 16.4 and 11.5 cal. ka BP. The lake level drop that occurred at ca 8.7 ka BP is well documented by both bioproxies.","palabras_clave":"DIATOMS|CHIRONOMIDS|PALEOCLIMATIC RECONSTRUCTION|LATE GLACIAL|HOLOCENE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64706,"idioma_id":5,"titulo":"Caracterización de la deserción universitaria en la UNRN utilizando Minería de Datos. Un caso de estudio","resumen":"En el ámbito dela Universidad Nacional de Río Negro (UNRN), y en particular en la SedeAtlántica desde la Licenciatura en Sistemas, es una creciente preocupación elfenómeno de deserción y desgranamiento que se ha podido apreciar en los cuatroprimeros años de vida de la Institución. El presente trabajo describe el proceso deidentificación de las características más relevantes del problema a través delas cuales, utilizando técnicas de Minería de Datos (DM), puede obtenerse unmodelo de la deserción universitaria en la unidad académica mencionada. Paraidentificar las características más relevantes se propone analizar, luego delpreprocesamiento de los datos,&nbsp; lasproyecciones de los atributos en las clases o respuestas esperadas. Suaplicación a los datos de los alumnos de las carreras de grado de la UNRN haofrecido resultados satisfactoriospermitiendo efectuar recomendaciones tendientes a reducir el porcentaje dealumnos que abandona la carrera.","palabras_clave":"SELECCION DE ATRIBUTOS|PROYECCION DE ATRIBUTOS|MINERIA DE DATOS|DESERCION UNIVERSITARIA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64707,"idioma_id":5,"titulo":"El test de diatomeas en el diagnóstico de muerte por sumersión.","resumen":"Las diatomeas son conocidas en el ámbito científico como valiososindicadores ambientales y paleoambientales. En los últimos años, varios paísesde Europa, Asia y América del Norte las han adoptado como herramienta diagnósticade rutina en casos de muerte por sumersión. En Sudamérica esto no ha ocurrido,fundamentalmente por la poca difusión que se le ha dado a esta práctica y estoa su vez es consecuencia directa de la escasez de especialistas. En estacontribución se aportan datos y herramientas para la utilización del conocimientode las diatomeas en el diagnóstico de muerte por sumersión y se presenta unprotocolo para la toma de muestras de tejidos cadavéricos que debe sercomunicado a los profesionales o técnicos encargados de hacerlo a fin de evitarcontaminaciones que enmascaren los resultados del análisis.","palabras_clave":"AHOGAMIENTO|CIENCIAS FORENSES|DIATOMEAS|SUMERSION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64708,"idioma_id":4,"titulo":"Activated ALPHA 2-macroglobulin induces Müller glial cell migration by regulating MT1-MMP activity through LRP1","resumen":"In retinal proliferative diseases, Müller  glial cells (MGCs) acquire migratory abilities. However,  the mechanisms that regulate this migration remain  poorly understood. In addition, proliferative disorders  associated with enhanced activities of matrix metalloprotease  2 (MMP-2) and MMP-9 also present increased  levels of the protease inhibitor 2-macroglobulin (2M)  and its receptor, the low-density lipoprotein receptorrelated  protein 1 (LRP1). In the present work, we  investigated whether the protease activated form of  2M, 2M*, and LRP1 are involved with the MGC  migratory process. By performing wound-scratch migration  and zymography assays, we demonstrated that  2M* induced cell migration and proMMP-2 activation  in the human Müller glial cell line, MIO-M1. This  induction was blocked when LRP1 and MT1-MMP were  knocked down with siRNA techniques. Using fluorescence  microscopy and biochemical procedures, we  found that 2M* induced an increase in LRP1 and  MT1-MMP accumulation in early endosomes, followed  by endocytic recycling and intracellular distribution of  MT1-MMP toward cellular protrusions. Moreover,  Rab11-dominant negative mutant abrogated MT1-MMP  recycling pathway, cell migration, and proMMP-2 activation  induced by 2M*. In conclusion, 2M*, through  its receptor LRP1, induces cellular migration of Müller  glial cells by a mechanism that involves MT1-MMP  intracellular traffic to the plasma membrane by a Rab11-dependent recycling pathway.?Barcelona, P. F.,  Jaldín-Fincati, J. R., Sánchez, M. C., Chiabrando, G. A.  Activated 2-macroglobulin induces Müller glial cell  migration by regulating MT1-MMP activity through  LRP1. FASEB J. 27, 000?000 (2013). www.fasebj.org","palabras_clave":"ENDOCYTOSIS|TRAFFIC|PROTEASES|RETINA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64709,"idioma_id":4,"titulo":"Sleep and quality of life in urban poverty: the impact of a slum-housing upgrading program","resumen":"Study Objectives: To evaluate the impact of a housing transition on sleep quality and quality of life in slum dwellers, participating in a slum-housing upgrading program.  Design: Observational before-and-after study with a convergent-parallel mixed method design.  Setting: Five slums located in the Metropolitan Area of Buenos Aires, Argentina.  Participants: A total of 150 slum dwellers benefited by a housing program of a non-profit organization (?TECHO?).  Interventions: Participants moved from their very low quality house to a basic prefabricated 18m2 modular house provided by ?TECHO?.  Measurements and Results: The Pittsburgh sleep quality index (PSQI) and WHO quality of life (QOL) brief scale were administered before and after housing upgrading. Data about housing conditions, income, education, sleeping conditions, and cardiovascular risk were also collected. Semi-structured interviews were used to expand and nuance quantitative  data obtained from a poorly educated sample. Results showed that sleep quality significantly increased after the housing program (z=-6.57, p<0.001). Overall QOL (z=-6.85, p<0.001), physical health domain (z=-4.35, p<0.001), psychological well-being domain (z=-3.72, p<0.001) and environmental domain (-7.10, p<0.001) of QOL were also improved. Interviews demonstrated the importance of serenity for improving QOL.  Conclusions: A minimal improvement of basic housing can significantly increase sleep quality and quality of life among slum dwellers. Understanding sleep and daily life conditions in informal urban settlements could help to define what kind of low cost intervention may  improve sleep quality, quality of life, and reduce existent sleep disparity.","palabras_clave":"SLEEP|QUALITY OF LIFE|URBAN POVERTY|HOUSING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64710,"idioma_id":4,"titulo":"Circadian rhythm of autonomic cardiovascular control during Mars500 simulated mission to Mars","resumen":"Introduction: The Mars500 project was conceived to gather knowledge about the psychological and physiological effects of living in an enclosed environment during the 520 days as would be required for a real mission to Mars. Our objective was to investigate the circadian profile of heart rate variability in the context of the Mars500 study. Methods: 24-h  EKG records were obtained before, during and after confinement from the six crew members that participated in the mission. Autonomic activity was evaluated through time and frequency domain indexes of heart rate variability (HRV) analysis. Circadian rhythmicity was assessed both by averaging hourly HRV along wake and sleep scheduled periods and  by fitting a 24-hour harmonic to the hourly means. Results: During confinement, wake HRV showed (mean ± SE) a progressive increase in mean RR interval (from 778 ± 24 ms to 916 ± 42 ms), and in the amplitude (values are wavelet power coefficients) of very low (from 13.3 ± 0.3 to 14.1 ± 0.2) and high (from 7.8 ± 0.4 to 8.3 ± 0.3) frequency components.  During sleep, the relative amplitude of the high frequency component of HRV decreased (from 11.8 ± 1.6 nu to 9.4 ± 1.8 nu). Overall, sleep-wake differences of HRV showed a progressive decrease of the relative amplitude of the high frequency component. Also, circadian HRV rhythms were dampened during confinement. Discussion: Data revealed diminished amplitude of the rest-activity pattern of autonomic nervous system  parasympathetic function. Reduced daylight exposure and mood changes could account for this observation.","palabras_clave":"SPACE PHYSIOLOGY|CONFINEMENT|SLEEP|HEART RATE VARIABILITY|WAVELETS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64711,"idioma_id":4,"titulo":"Stiffness of lipid monolayers with phase coexistence","resumen":"The surface dilational modulus -or compressibility modulus- has been previously studied for monolayers composed of pure materials, where a jump in this modulus was related with the onset of percolation as a result of the establishment of a connected structure at the molecular level.   In this work, we focused on monolayers composed of two components of low lateral miscibility. Our aim was to investigate the compressibility of mixed monolayers at pressures and compositions in the two-phase region of the phase diagram, in order to analyze the effect of the mechanical properties of each phase on the stiffness of the composite. In nine different systems with distinct molecular dipoles and charges, the stiffness of each phase and the texture at the plane of the monolayer were studied. In this way, we were able to analyze the general compressibility of two-phase lipid monolayers, regardless of the properties of their constituent parts.  The results are discussed in the light of the following two hypotheses: first, the stiffness of the composite could be dominated by the stiffness of each phase as a weighted sum according to the percentage of each phase area, regardless of the distribution of the phases in the plane of the monolayer. Alternatively, the stiffness of the composite could be dominated by the mechanical properties of the continuous phase. Our results were better explained by this latter proposal as in all the analyzed mixtures it was found that the mechanical properties of the percolating phase were the determining factors. The value of the compression modulus was closer to the value of the connected phase than to that of the dispersed phase, indicating that the bidimensional composites displayed mechanical properties that were related to the properties of each phases in a rather complex manner.","palabras_clave":"Monolayer reology|Composites stiffnes|Compressibility modulus"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64712,"idioma_id":5,"titulo":"Velocidades Sísmicas de las Unidades Aflorantes en el Flanco Occidental de la Sierra de la Invernada, Precordillera de San Juan","resumen":"En este trabajo se muestran determinaciones de velocidades de ondas sísmicas P (Vp) en un sector (Reserva Don Carmelo) del  flanco occidental de la sierra de la Invernada, Departamento Ullúm, Provincia de San Juan. Las velocidades obtenidas con el  método de refracción sísmica indican valores de Vp de 550; 1.670 y 4.134 m\/s y espesores de 1 m y 7 m aproximadamente, para  un modelo de dos capas apoyadas sobre un substrato correspondiente a la mayor velocidad. Las unidades aflorantes reconocidas  abarcan grauvacas y areniscas muy bien compactadas que se correlacionan con los valores más altos de Vp determinados en el experimento  geofísico para los niveles interpretados a mayor profundidad. Asimismo los valores más bajos de Vp son consistentes  con material de tipo aluvional situado en las capas sísmicas más superficiales (< 7m de profundidad). Otras unidades relevadas  en el terreno indican afloramientos de diabasas y gabros, los cuales no han sido explorados geofísicamente. Sin embargo, a partir  de la comparación de las litologías y velocidades sísmicas Vp observadas con bases de datos globales, es posible predecir la  continuación en profundidad de las litologías máficas con velocidades sísmicas de Vp aún mayores que aquellas determinadas  en superficie. Estos resultados son de interés debido a que ayudan a identificar una posible faja ofiolítica en Precordillera, en un  sector donde el espesor cortical actual estimado en 64 km aproximadamente, estaría engrosado.","palabras_clave":"REFRACCION SISMICA|BASAMENTO|ONDAS P|OFIOLITA|PRECORDILLERA|PETROLOGIA|GEOFISICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64713,"idioma_id":5,"titulo":"'Cuantificación e (in)suficiencia argumentativa: construcciones con 'para' de contraexpectativa'","resumen":"Como señala Kovacci (1997), el período con si condicional resulta un esquema sintáctico adecuado para destacar el paralelismo entre prótasis y apódosis, ya sea como correspondencia comparativa, ya sea como relación de contraposición que permita justificar la aserción, de naturaleza inferencial deductiva, que el locutor realiza en el condicionado. Según la autora, en el primer caso, predomina la interpretación condicional; pero en el segundo, se suma un matiz concesivo. En lo que concierne a la expresión de la finalidad con para, específicamente cuando se manifiesta la noción de \u2018suficiencia\u2019, también el esquema oracional se vuelve adecuado para desencadenar interpretaciones más ligadas a la mera correspondencia entre lo enunciado en cada constituyente, o más vinculadas con algún grado de hipótesis, propio de las condicionales, o un cierto nivel de contraexpectativa, algo típico de las concesivas. Este último grupo de construcciones con para, en ocasiones con infinitivo simple pero más frecuentemente con infinitivo compuesto, se convierte en un mecanismo que le permite al locutor incorporar la voz de otro ego en su discurso, al mismo tiempo que reorienta la dirección argumentativa que adopta, con la que se distancia de opiniones divergentes.","palabras_clave":"Construcciones con para + infinitivo|Cuantificación|Contraexpectativa|(In)suficiencia  argumentativa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64714,"idioma_id":4,"titulo":"Cross-Sensitization Between Cocaine and Acute Restraint Stress is Associated with Sensitized Dopamine but not Glutamate Release in the Nucleus Accumbens","resumen":"Repeated administration of psychostimulant drugs or stress can elicit a sensitized response to the stimulating and reinforcingproperties of the drug. Here we explore the mechanisms in the nucleus accumbens (NAc) whereby an acute restraint stressaugments the acute locomotor response to cocaine. This was accomplished by a combination of behavioral pharmacology, microdialysismeasures of extracellular dopamine and glutamate, and Western blotting for GluR1 subunit of the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) glutamate receptor (AMPAR). A single exposure to restraint stress 3 weeks beforetesting revealed that enduring locomotor sensitization to cocaine was paralleled by an increase in extracellular dopamine in thecore, but not the shell subcompartment, of the NAc. Wistar rats pre-exposed to acute stress showed increased basal levels ofglutamate in the core, but the increase in glutamate by acute cocaine was blunted. The alterations in extracellular glutamateseem to be relevant, as blocking AMPAR by 6-cyano-7-nitroquinoxaline-2,3-dione microinjection into the core prevented both thebehavioral cross-sensitization and the augmented increase in cocaine-induced extracellular dopamine. Further implicating glutamate,the locomotor response to AMPAR stimulation in the core was potentiated, but not in the shell of pre-stressed animals, andthis was accompanied by an increase in NAc GluR1 surface expression. This study provides evidence that the long-term expressionof restraint stress-induced behavioral cross-sensitization to cocaine recapitulates some mechanisms thought to underpin thesensitization induced by daily cocaine administration, and shows that long-term neurobiological changes induced in the NAc byacute stress are consequential in the expression of cross-sensitization to cocaine.","palabras_clave":"AMPA receptor surface expression|locomotor activity|microdialysis|nucleus accumbens core and shell|psychostimulant|stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64715,"idioma_id":5,"titulo":"La Biblioteca de la Asociación del Consejo de Mujeres.","resumen":"REVISTA INDEXADA EN LATINDEX, BINPAR-CAYCIT, RED DE INFORMACION DE LA ALADI. El artículo se refiere a la primera Biblioteca Popular de Rosario y su evolución en el siglo XX a través del la filial Rosario de la Asociación de la Biblioteca del Consejo de Mujeres, fundada en 1925. Sobre fuentes inéditas de época se estudia la conformación de una red cultural y social en el marco de las relaciones la elite dirigente de la ciudad puerto de Rosario. ","palabras_clave":"ROSARIO|CULTURA|BIBLIOTECA|SOCIEDAD"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64716,"idioma_id":5,"titulo":"Servicios de regulación y prácticas de manejo: aportes para una horticultura de base agroecológica","resumen":"La importancia de las prácticas de manejo de base agroecológica ha sido ampliamente tratada en la literatura. La relación entre éstas y los servicios de regulación en los agroecosistemas es un aporte esencial en el desarrollo de sistemas productivos sustentables. Este trabajo analiza la relación entre las prácticas de manejo agroecológico en horticultura y los diferentes servicios de regulación a escala predial. Se pretende establecer un marco conceptual que facilite a técnicos, investigadores y gestores, avanzar en alternativas tecnológicas que promuevan los procesos de transición agroecológica. El conocimiento de las interacciones entre prácticas y servicios es necesario para la toma de decisiones relativa a la gestión de los agroecosistemas y la conservación de la biodiversidad, en particular en los sistemas agrarios.","palabras_clave":"Agroecosistemas|Agrobiodiversidad|Suelos|Transición Agroecológica"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64717,"idioma_id":5,"titulo":"Universidad y política exterior. La formación de expertos y diplomáticos en la Facultad de Ciencias Económicas, Comerciales y Políticas de Rosario, 1920-1968","resumen":"Publicación indizada en LATINDEX CATALOGO. NIVEL 1 DE EXCELENCIA. y CLASIFICACIÓN INTEGRADA DE REVISTAS CIENTÍFICAS, GRUPO C. La Facultad de Ciencias Económicas, Comerciales y Políticas de Rosario, dependiente de la Universidad Nacional del Litoral fue, a partir de 1928, la única casa de estudios universitarios en Sudamérica (y una de las pocas en el mundo) donde además de cursarse la carrera de grado en Diplomacia y Servicio Consular  podía obtenerse  el título de doctor en Diplomacia. Este trabajo aborda la conjunción de factores que así lo posibilitaron; la incidencia de las sucesivas etapas en las que se puede dividir la historia de  la Facultad en las preferencias temáticas, impronta e inquietudes observables en las más de ochenta tesis doctorales en diplomacia aprobadas entre 1932 y 1968, y en la evolución del prestigioso Instituto de Derecho Internacional. Por otra parte, el análisis de los considerandos y argumentaciones de las diversas peticiones y propuestas efectuadas por estudiantes y docentes de dicha Facultad solicitando a los gobiernos y al Congreso de la Nación la reglamentación de la carrera diplomática y la incorporación de graduados universitarios en el servicio exterior de la Nación, es entendido en este artículo como un recurso para identificar las suscripciones formales a determinados proyectos de país.","palabras_clave":"HISTORIA|DIPLOMACIA|UNIVERSIDAD|ROSARIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64718,"idioma_id":4,"titulo":"Comprehensive Feature Extraction for Objective Dynamic Gait Index Assessment of Risk of Falls in the Elderly","resumen":"The evaluation and understanding of human balance and risk of falls, particularly in the elderly, is a growing field of study. Complementeing classic clinical tests (relying on comprehensive, although mostly qualitative assessments) with new devices that acquire precise patterns associated to the balance process, appears as a great oportunity in this area. Objective features can be obtained from these patterns, summarizing relevant data about balance and gait processes. This work presents results from the Dynamic Gait Index assessment over 42 subjects, while wearing a specially designed 3D-linear acceleration sensing device. Withknowledge about the test, features related to step frequency, energy expenditure and changes in signal shape were defined and obtained from the three acceleration signals. The evolution of these features over time is of interest, so a moving-window strategy was implemented. Signalprocessing strategies were tested and improved, and results were correlated with the clinical scores given by the physicians to better understand them, initially test their reliability and select the most suitable ones. Results showed promise for this strategy in providing uniform, meaningful and new interpretations about different processes involved in the stages of the test, opening the possibility to develop custom user interfaces for clinical use.","palabras_clave":"Dynamic Gait Index|Risk of Falls"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64719,"idioma_id":5,"titulo":"DIAGNÓSTICO DE INFECCIÓN POR Trypanosoma cruzi EN CENTROS DE ATENCIÓN PRIMARIA DE SALTA, ARGENTINA","resumen":"    La enfermedad de Chagas causada por el hemoflagelado Trypanosoma cruzi, constituye un grave problema de Salud Pública. En Argentina, se controla sistemáticamente la sangre a transfundir, la donación de órganos y se ha disminuido notablemente la transmisión vectorial. El objetivo de este proyecto fue implementar el uso del equipo para recolección de sangre capilar y conservación en glicerina (Serokit) en los servicios de enfermería de los Centros de Atención Primaria de la Salud en la ciudad de Salta a fin de conocer la seroprevalencia de infección por Trypanosoma cruzi en pacientes que concurren a los mismos. Para ello se realizó el par serológico HAI y ELISA en las muestras conservadas en Serokit y luego en muestras de sangre tomadas por punción venosa para confirmación. Durante dos años de trabajo se analizaron 1647 pacientes que concurrieron a 28 Centros de Salud, resultando 1,7% (29\/1647) seropositivos. El Valor Predictivo Positivo fue 93,50% y el Valor Predictivo Negativo fue 99,8%. Todos los niños seropositivos fueron tratados con Benznidazol. Se concluye que el uso de SEROKIT para la toma y conservación de muestras para posterior diagnóstico de infección por Trypanosoma cruzi es recomendable en Centros de Atención Primaria de la Salud que no cuentan con laboratorio.     ","palabras_clave":"ATENCIÓN PRIMARIA DE LA SALUD|ENFERMEDAD DE CHAGAS|SEROLOGIA|NIÑOS|TRYPANOSOMA CRUZI|PREVENCIÓN Y CONTROL"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64720,"idioma_id":4,"titulo":"Memory expression is independent of memory labilization\/reconsolidation","resumen":"There is growing evidence that certain reactivation conditions restrict the onset of both the destabilization  phase and the restabilization process or reconsolidation. However, it is not yet clear how changes in  memory expression during the retrieval experience can influence the emergence of the labilization\/  reconsolidation process. To address this issue, we used the context-signal memory model of Chasmagnathus.  In this paradigm a short reminder that does not include reinforcement allows us to evaluate memory  labilization and reconsolidation, whereas a short but reinforced reminder restricts the onset of such a  process. The current study investigated the effects of the glutamate antagonists, APV (0.6 or 1.5 lg\/g) and  CNQX (1 lg\/g), prior to the reminder session on both behavioral expression and the reconsolidation process.  Under conditions where the reminder does not initiate the labilization\/reconsolidation process, APV  prevented memory expression without affecting long-term memory retention. In contrast, APV induced  amnesic effects in the long-term when administered before a reminder session that triggers reconsolidation.  Under the present parametric conditions, the administration of CNQX prior to the reminder that  allows memory to enter reconsolidation impairs this process without disrupting memory expression.  Overall, the present findings suggest that memory reactivation ? but not memory expression- is necessary  for labilization and reconsolidation. Retrieval and memory expression therefore appear not to be  interchangeable concepts.","palabras_clave":"memory|retrieval|reconsolidation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64721,"idioma_id":5,"titulo":"Revista Electrónica de Psicología Política: 10 años colaborando a la definición de la disciplina","resumen":"El presente artículo pretende hacer una reseña descriptiva de las características más relevantes de la Revista Electrónica de Psicología Política (Argentina), a lo largo de sus 10 años de publicación en línea. Para tal descripción, fueron tenidas en cuenta las temáticas abordadas por los artículos publicados en la revista, los autores según su origen y filiación institucional, y las características de difusión de la publicación en cuanto a diversidad idiomática y gratuidad de acceso a sus artículos.","palabras_clave":"Revista Electrónica de Psicología Política |Psicología política |Latinoamérica|Temas"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64722,"idioma_id":4,"titulo":"Involvement of nNOS\/NO\/sGC\/cGMP signaling pathway in cocaine sensitization and in the associated hippocampal alterations: does phosphodiesterase 5 inhibition help to drug vulnerability?","resumen":"Repeated cocaine administration induces behavioral sensitization in about 50 % of treated animals. Nitric oxide could be involved in the acquisition and maintenance of behavioral cocaine effects, probably by activation of nNOS\/NO\/sGC\/cGMP signaling pathway, since inhibition of the nNOS enzyme attenuates development of sensitization in rats. On the other hand, increased cGMP availability by Phosphodiesterase 5 inhibitors has been correlated to the misuse and recreational use of these agents, and also to the concomitant use with illicit drugs in humans. Hippocampus is an important brain region for conditioning to general context previously associated to drug availability, influencing drug-seeking behavior and sensitization. Moreover, cocaine and other drugs of abuse can affect the strength of glutamate synapses in this structure, lastly modifying neuronal activity in main regions of the reward circuitry. In the present work we attempted to determine whether the pharmacological manipulation of nNOS\/NO\/sGC\/cGMP signaling pathway altered changes induced by repeated cocaine exposure. We demonstrate a key role of the nNOS activity and NO\/sGC\/cGMP signaling pathway in the development of cocaine sensitization and in the associated enhancement of hippocampal synaptic transmission.","palabras_clave":"Oxido Nitrico|Cocaina|Hipocampo|Sildenafil"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64723,"idioma_id":4,"titulo":"Monte Carlo simulation of gold and silver nanoparticles in interaction with strongly surfactant media","resumen":"The melting and subsequent freezing of Ag and Au nanoclusters consisting of 55 and 147 atoms and interacting with a surfactant medium is studied by Monte Carlo simulations using the embedded- atom method. In our model, the interaction with the surfactant medium is represented by an additional energy term (Q) associated to a surface atom. We observe a decrease in the transition temperature (melting point) with the increase of the value of Q. In some of the systems, before the melting, a solid?solid structural transition takes place as the temperature increases. For large values of the parameter Q, the structures obtained from the freezing tend to have morphologies that have a larger fraction of atoms at the surface and which would be otherwise unstable.","palabras_clave":"Metal Nanoparticle|Melting|Monte Carlo Simulation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64724,"idioma_id":4,"titulo":"New  technologies for big multimedia data treatment","resumen":"With the technology advance and the growth of Internet, the information that can be found in this net, as wellas the number of users that access to look for specific data is bigger. Therefore, it is desirable to have a search system that allows to retrieve information at a reasonable time and in an efficient way. In this paper we show two computing paradigms appropriate to apply in the treatment of large amounts of data consisting of objects such as images, text, sound and video, using hybrid computing over MPI+OpenMP and GPGPU.The proposal is developed through experience gained in the construction of various indexes and the subsequent search, through them, of multimedia objects.","palabras_clave":"METRIC SPACE|HYBRID COMPUTATION|GPU|PARALLEL SEARCHING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64725,"idioma_id":4,"titulo":"Suffix Array Performance Analysis for Multi-core Platforms","resumen":"Performance analysis helps to understand how a particular invocation of an algorithm executes. Using the information provided by specific tools like the profiler tool Perf or the Performance Application Programming Interface (PAPI), the performance analysis process provides a bridging relationship between the algorithm execution and processor events according to the metrics defined by the developer. It is also useful to find performance limitations which depend exclusively on the code.  Furthermore, to change an algorithm in order to optimize the code requires more than understanding of the obtained performance. It requires understanding the problem being solved. In this work we evaluate the performance achieved by a suffix array over a 32-core platform. Suffix arrays are efficient data structures for solving complex queries in a number of applications related to text databases, for instance, biological databases. We perform experiments to evaluate hardware features directly aimed to parallelize computation. Moreover, according to the results obtained by the performance evaluation tools, we propose an optimization technique to improve the use of the cache memory. In particular, we aim to reduce the number of cache memory replacement performed each time a new query is processed.","palabras_clave":"Multicore.|Suffix array."},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64726,"idioma_id":4,"titulo":"short transmembrane domains with high volume exoplasmic halves determine retention of type II   membrane proteins in the Golgi complex","resumen":"It is still unclear why some proteins that travel along the secretory pathway are retained in the Golgi complex while others follow their way to the plasma membrane (PM). Recent bioinformatic analyses on a large number of single spanning membrane proteins support the hypothesis that specific features of the Trans-Membrane Domain (TMD) are relevant to the sorting of these proteins to particular organelles. Here we experimentally test this hypothesis  for Golgi and PM proteins. Using the Golgi SNARE Sft1 and the PM SNARE Sso1 from Saccharomyces cerevisiae as model proteins, we modified the length of their TMDs and the volume of their ectoplasmic hemi-TMD, and determined their subcellular localization both in yeast and mammalian cells. We found that short TMDs with voluminous ectoplasmic hemi- TMDs confer Golgi membrane residence, while TMDs having less voluminous ectoplasmic hemi-TMDs, being either short or long, confer PM residence to these proteins. Results indicate that the shape of the exoplasmic hemi-TMD, in addition to the length of the entire  TMD, determine retention in the Golgi or exit to the PM of Type II membrane proteins.","palabras_clave":"TRANSMEMBRANE DOMAIN|GOLGI|TRAFFIC|YEAST"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64727,"idioma_id":4,"titulo":"Comparative morpho biochemical responses of sunflower lines sensitive and tolerant to water stress","resumen":"Water stress is likely the most important factor that adversely affects plant growth and development. In our study two inbred lines of sunflower (Helianthus annuus L.) with contrasting behavior to water deficit, the inbred lines B59, sensitive, and B71, tolerant, were subjected to water stress and compared in terms of growth parameters, relative water content, photosynthetic pigments and osmo-compatibles contents. Mannitol treatment generated a moderate water stress which resulted in a relative water content decline in both lines. In aerial part of B59 and B71 stressed-seedlings, a dramatic reduction of osmotic potential was observed, although in B71 the osmotic potential was 1.8-fold lower than in B59. The B71 line recovered the osmotic potential to control values after 8 d of re-watering. Several morphological parameters were altered by the water stress. Shoot and root relative fresh weight decreased in both lines under water stress, although B71 showed a minor drop. However, the root was less affected than the shoot in stress condition and recovery period. Water stress was affected in a greater proportion shoot dry weight (DW) than root DW in B59 and B71 lines. Nevertheless, a significant increase in root DW\/shoot DW ratio was observed in stressed seedlings. A higher reduction in the individual leaf area was observed in B71 line under stress. The basal chlorophyll content was higher in the tolerant line B71 than in the sensitive B59, and carotenoids showed a high content in B71 control and stressed seedlings. Under water stress, the aerial and root proline content increased in the tolerant B71 line. Glycinebetaine decreased significantly in the aerial part of both lines in response to water stress indicating that this compound is not the main osmo-regulator. In conclusion, B71 has suitable mechanisms to enable it to respond more effectively to moderate water stress.","palabras_clave":"Growth Parameters|Osmo-compatibles Solutes|Photosynthetic Pigments Sunflower|Water Stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64728,"idioma_id":5,"titulo":"La eficacia atencional. Estudio normativo en niños escolarizados de Mendoza","resumen":"El propósito de este trabajo fue evaluar la eficacia atencional en alumnos que asisten aestablecimientos escolares públicos de gestión estatal de ámbito urbano y urbanomarginado,de nivel primario del Gran Mendoza y, a su vez, elaborar las normas localesde la Escala Magallanes de Atención Visual (EMAV). La EMAV es de gran interéspara la evaluación neuropsicológica de las funciones atencionales. La muestra deestandarización se obtuvo por muestreo aleatorio estratificado con procedimientos dedistribución proporcional y estuvo conformada por 5779 escolares entre 6 y 14 años deedad, de primero a séptimo grado, de diecisiete establecimientos educativos. Se utilizóla siguiente fórmula para evaluar la eficacia atencional: [(aciertos+omisiones) \/ aciertosposibles]. Finalmente, se presentan los baremos de la escala EMAV, discriminados poredad, puntaje parcial y total de ejecución.","palabras_clave":"MECANISMO ATENCIONAL|EFICACIA ATENCIONAL|NIÑOS ESCOLARIZADOS|INSTRUMENTOS DE EVALUACION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64729,"idioma_id":5,"titulo":"?Grupos sociales rurales del Chaco austral argentino. Una descripción de sus condiciones de vida, costumbres de esparcimiento y problemáticas, durante las primeras décadas del siglo XX?.","resumen":"Este trabajo aborda algunos aspectos de la vida cotidiana de los habitantes del mundo rural del Chaco, en el norte de Argentina, durante el período en que fue un Territorio Nacional, particularmente en las cuatro primeras décadas del siglo XX.&nbsp;Se examinan especialmente las costumbres y hábitos de esparcimiento de sectores sociales que, por la propia naturaleza de sus labores u oficios, y por residir en ámbitos alejados de los centros poblados, utilizaron espontáneas maneras de entretenimiento que a veces tuvieron consecuencias negativas para la sanidad, la seguridad, y la productividad laboral. Se analizan así mismo las percepciones y las medidas tomadas por la clase dirigente respecto a esta problemática.","palabras_clave":"RURALIDAD|CHACO|PRODUCTIVIDAD|TERRITORIOS NACIONALES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64730,"idioma_id":5,"titulo":"Estudio descriptivo sobre la utilización de plantas como alternativas terapeuticas.","resumen":"Objetivo: Conocer el grado de utilización, Prevalencia (P) de uso, de Plantas Medicinales (PM) como alternativa terapéutica en la ciudad de Córdoba, Argentina.  Métodos: Se realizó un estudio Transversal aplicando una encuesta autoadministrada en 12 barrios de la ciudad de Córdoba elegidos aleatoriamente, 4 de cada clase social. El estudio se llevó a cabo entre mayo y agosto de 2009. Los datos fueron procesados utilizando el programa Epi info 3.5.1 y cuando fue factible se aplicó el test Chi cuadrado (&#967;2) para determinar asociación entre variables.  Resultados: La P de uso de PM como alternativa terapéutica fue de 53,30% (IC95%= 49,10% - 57,40%). Luego del análisis del &#967;2 se determinó que las PM son más usadas por el sexo femenino (54,60%), las personas de clase media (56,85%) y en forma similar por los grupos etarios correspondientes a Senectud (67,59%) y Adultez Media (62,50%). El 69,36% de los consumidores fueron inducidos por familiares o conocidos y sólo el 23,12%, por un profesional del equipo de salud. El 86,07% (IC95%= 83,57% - 88,57%) de las personas que consumieron PM refieren haber obtenido un buen resultado; sólo el 4,9% (IC95%= 2,87% - 6,87%) de los encuestados respondieron haber padecido efectos adversos luego de la utilización de diversas PM.  Conclusiones: Si se suma al 53,30% de personas que usan PM un 29,17% que aunque respondió que no usa PM afirma que consume diversas especies para ?ayudar a la digestión?, la P asciende a 81,90%, resultado coherente con el mencionado por la OMS.","palabras_clave":"Plantas Medicinales|Prevalencia|Argentina"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64731,"idioma_id":5,"titulo":"Efecto del seguimiento farmacoterapéutico a pacientes con diabetes mellitus tipo 2 en un servicio de atención primaria de Córdoba (Argentina)","resumen":"Introducción: El farmacéutico puede desempeñar un importante papel en el seguimiento farmacoterapéutico (SFT) de los pacientes con diabetes mellitus tipo 2 (DM2), y para conseguir este objetivo es fundamental la coordinación entre médicos y farmacéuticos.Objetivos: Determinar el efecto del SFT en pacientes que se encuentran en el Programa Municipal de Detección Precoz de DM2 en la ciudad de Alta Gracia (Argentina).Material y métodos: Estudio de seguimiento longitudinal, prospectivo, durante 6 meses (desde julio hasta diciembre de 2011). Se empleó la metodología Dáder para efectuar el SFT y la clasificación MEDAFAR para registrar las derivaciones farmacoterapéuticas. La adherencia al tratamiento farmacológico se determinó comparando los retiros mensuales de medicación.Resultados: Participaron 101 pacientes, 74 en el grupo de intervención (GI) y 27 en el grupo de comparación (GC). Se realizaron 198 intervenciones farmacéuticas en 74 pacientes, de las cuales 63 fueron del tipo farmacéutico-paciente, resolviéndose un 77,8%, y 135 fueron del tipo farmacéutico-paciente-médico, resolviéndose un 80,7%.Se observó una disminución del 29,9% de la glucemia en ayunas en el GI y un aumento del 28,9% en el GC. Las derivaciones farmacoterapéuticas fueron en su mayoría de información (58,6%). La adherencia al tratamiento farmacológico aumentó en el GI un promedio de un 14,8%.Conclusiones: El SFT en pacientes con DM2 permitió una resolución elevada de resultados negativos asociados a la medicación, mejoras en los valores de glucemia en ayunas, un aumento de la adherencia al tratamiento farmacológico de los pacientes y una adecuada coordinación entre los profesionales a través del uso de la clasificación MEDAFAR.","palabras_clave":"DIABETES MELLITUS TIPO 2|ATENCIÓN PRIMARIA DE SALUD|SERVICIOS FARMACÉUTICOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64732,"idioma_id":5,"titulo":"Pacientes diabéticos sin cobertura de salud: utilización de medicamentos, adherencia y complicaciones derivadas de su patología de base","resumen":"Objetivo: realizar un Estudio de Utilización deMedicamentos de los pacientes diabéticos atendidos en elHospital Josefina Prieur (HJP) de la ciudad de Villa Allende(Córdoba, Argentina), determinar consumo de medicamentosantidiabéticos, grado de adherencia al tratamiento y pacientesafectados por complicaciones derivadas de la diabetes.Método: Estudio observacional, descriptivo y transversal,sobre pacientes diabéticos atendidos en el HJP que pertenecenal Programa Córdoba Diabetes (ProCorDia) durante los mesesde Mayo a Diciembre de 2011. El consumo de medicamentosse determinó en DDD por 1000 habitantes por día (DHD).La adherencia se estableció del número de dispensacionesrealizadas a cada paciente durante los ocho meses. Lapresencia de complicaciones se obtuvo observando en elregistro de entrega de medicamentos a pacientes que retiranotros medicamentos además de antidiabéticos.Resultados: Sobre 98 pacientes, 73 presentaron algún tipode complicación. El consumo de medicamentos antidiabéticosen DHD fue: Insulina Corriente 1,990; Insulina NPH 6,244;Glibenclamida 2,826; Glicazida 0,281; Metformina 2,497.Conclusiones: Del total de medicamentos antidiabéticosdispensados, el 40,50% correspondió al subgrupo A10B(hipoglucemiantes orales) y el 59,50% al subgrupo A10A(Insulinas). Se observó una buena adherencia al tratamiento (media=81.63%, moda=100%). El 74,49% de los pacientes presentó complicaciones.","palabras_clave":"DIABETES MELLITUS (DM)|ESTUDIOS DE UTILIZACIÓN DE MEDICAMENTOS (EUM)|DOSIS DIARIA DEFINIDA (DDD)|PROGRAMA CÓRDOBA DIABETES (PROCORDIA)|COMPLICACIONES DE LA DIABETES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64733,"idioma_id":4,"titulo":"Mean loads on vaulted canopy roofs","resumen":"The vaulted canopy roof (VCR) is a widespread structure used in vast areas of South America, especially in parts of Argentina, Brazil and Paraguay. Information about the aerodynamics of VCRs is scarce in the literature, and it is restricted to mean load coef&#64257;cients, which must be used in combination with the quasi-steady approach. Most of the existing data have been produced during the last decade in South America, but these data are not readily accessible because they were published in Spanish. This paper presents the results of mean wind load coef&#64257;cients on VCRs obtained in boundary layer tunnel tests, and the aerodynamics of VCRs are discussed by taking into account the data produced during the last decade.","palabras_clave":"WINDS LOADS|VAULTED ROOFS|CANOPY ROOFS|WIND TUNNEL|MEAN PRESSURES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64734,"idioma_id":4,"titulo":"Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine- sensitized adult rats.","resumen":"In previous studies we described that perinatal protein deprivation facilitates the development and expression of behavioral sensitization to cocaine. In this research, we explored whether the increased  reactivity observed in deprived (D) versus control (C) rats is also evident during drug-free withdrawal periods. Considering that activation of the extracellular signal-regulated protein kinase (ERK) is suggested  to be involved in cocaine-induced behavioral sensitization, we study the effects of perinatal protein deprivation on phosphorylated ERK2 (pERK2) protein levels in the NAc (core and shell) during different drug-free withdrawal periods. To induce behavioral sensitization, C- and D-rats received a daily injection of cocaine (5?10 mg\/kg, i.p.) for 7 days and locomotor activity was performed on days 1 and 7. Cocainesensitized  animals were left drug-free and pERK2 was assessed on withdrawal days (WD) 1, 4, 7 and 21. In the NAc core, cocaine induced ERK signaling pathway activation in a dose-dependent manner,and only D-rats showed a significant increase in pERK2 protein levels with the lowest dose of cocaine(5 mg\/kg). Moreover, sensitized C-rats with 10 mg\/kg showed an increase in pERK2 levels from WD7 while D-rats showed this activation on WD4, which remained increased on WD7 and 21. In contrast,in the NAc shell, only sensitized D-rats with cocaine 10 mg\/kg showed ERK2 activation on WD21. These results suggest that perinatal protein deprivation facilitates the molecular processes involved in neuronal plasticity occurring during withdrawal.","palabras_clave":"PERINATAL UNDERNUTRITION|BEHAVIORAL SENSITIZATION|EXTRACELLULAR SIGNAL-REGULATED KINASE|COCAINE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64735,"idioma_id":4,"titulo":"Boosting HIV treatment options: good news, new challenges.","resumen":"Editorial about the advantages and limitations of cobicistat as booster of protease inhibitors","palabras_clave":"COBICISTAT|ANTIRETROVIRAL|HIV"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64736,"idioma_id":4,"titulo":"Sexual risk-taking behaviour among HIV outpatients in Argentina.","resumen":"AbstractHIV prevention strategies must be based on evidence of risk behaviours among people with HIV infection. This study aimed to determine the demographic, behavioural and self-reported disease\/treatment variables that predict sexual risk behaviour, defined as unprotected intercourse with a partner of unknown or negative HIV status, among HIV-infected outpatients in Buenos Aires, Argentina. Two hundred consecutive outpatients (response rate 76.5%) participated in a self-complete cross sectional survey. The majority (49.5%) identified as heterosexual, and most were on antiretroviral therapy (ART) (75.5%). Undetectable viral load was currently achieved by 63%. Unprotected intercourse with a person of unknown or discordant status in the previous three months was reported by 20%. In multivariate analysis, risk was associated with lower distress from physical symptoms (p=0.012), greater distress from psychological symptoms (p=0.038) and being on treatment had borderline association with risk (p=0.058). The data reveal an important link between self-reported experience of disease, and treatment, with risk. Secondary prevention, care and treatment should not be conducted in isolation from each other if outcomes are to be optimised.","palabras_clave":"Sexual risk|outpatients|hiv"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64737,"idioma_id":4,"titulo":"Estimation of HIV-Testing Rates to Maximize Early Diagnosis-Derived Benefits at the Individual and Population Level","resumen":"Background: In HIV infection, initiation of treatment is associated with improved clinical outcom and reduced rate of sexualtransmission. However, difficulty in detecting infection in early stages impairs those benefits. We determined the minimumtesting rate that maximizes benefits derived from early diagnosis.Methods: We developed a mathematical model of HIV infection, diagnosis and treatment that allows studying bothdiagnosed and undiagnosed populations, as well as determining the impact of modifying time to diagnosis and testingrates. The model?s external consistency was assessed by estimating time to AIDS and death in absence of treatment as wellas by estimating age-dependent mortality rates during treatment, and comparing them with data previously reported fromCASCADE and DHCS cohorts.Results: In our model, life expectancy of patients diagnosed before 8 years post infection is the same as HIV-negativepopulation. After this time point, age at death is significantly dependent on diagnosis delay but initiation of treatmentincreases life expectancy to similar levels as HIV-negative population. Early mortality during HAART is dependent ontreatment CD4 threshold until 6 years post infection and becomes dependent on diagnosis delay after 6 years postinfection. By modifying testing rates, we estimate that an annual testing rate of 20% leads to diagnosis of 90% of infectedindividuals within the first 8.2 years of infection and that current testing rate in middle-high income settings stands close to10%. In addition, many differences between low-income and middle-high incomes can be predicted by solely modifying thediagnosis delay.Conclusions: To increase testing rate of undiagnosed HIV population by two-fold in middle-high income settings willminimize early mortality during initiation of treatment and global mortality rate as well as maximize life expectancy. Ourresults highlight the impact of achieving early diagnosis and the importance of strongly work on improving HIV testingrates.","palabras_clave":"HIV testing rates|early diagnosis|population level|estimation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64738,"idioma_id":4,"titulo":"Dissimilar expression of multidrug resistance mdr1 and bcrp by the replication of hepatitis C virus: role of the non structural 5A protein.","resumen":"Abstract    Multidrug resistance associated with the overexpression of ATP-dependent binding cassette (ABC) proteins is widely accepted as an important cause of treatment failure in patients with neoplastic or infectious diseases. Some of them play also a pivotal role in detoxification processes. Herein, we investigated the effect of hepatitis C virus (HCV) replication and nonstructural 5A (NS5A) protein on the expression and functional activity of two ABC transport proteins: MDR1 and BCRP. RT-quantitative real-time polymerase chain reaction (qPCR) was carried out for mdr1 and bcrp mRNAs in both Huh7 cells expressing NS5A and Huh7.5 cells containing either full-length- or subgenomic-HCV replicon systems. The functional activity of these pumps was studied by performing a dye efflux assay with DiOC2 and Rhodamine 123. A dose-dependent down-regulation of mdr1 expression was documented in Huh7 cells expressing the NS5A protein, as well as in both replicon systems. In contrast, a significant increase of bcrp expression in both systems was recorded, which were in full agreement with the dye efflux assay results. These results warrant further in vivo studies in HCV patients with cholestasis and\/or patients that are refractive to the pharmacotherapy due to the activity of these pumps.","palabras_clave":"HEPATITIS C VIRUS|NS5A|MDR1|BCRP"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64739,"idioma_id":4,"titulo":"Allergy training and immunotherapy in Latin America: results of a regional overview.","resumen":"One main practice gap in allergology that has been detected in several regions of the world is the application of specific immunotherapy (SIT). The prescription and practice of SIT should characterize allergologic specialists, but there are regional discrepancies in such practice. A detailed knowledge of the regulatory and legislation aspects and drawbacks would help improve and harmonize SIT practice.  OBJECTIVE:  To describe in Latin America the level of allergy training and the characteristics of the use of SIT, including the medical and legal aspects.  METHODS:  Three sources were used: a 24-item questionnaire sent to 22 allergologic leaders in 11 Latin American countries, 2 face-to-face meetings, and information from health authorities involved in the approval of medical substances.  RESULTS:  In 56% of countries, the specialty of allergology is a third-level care specialty and\/or a subspecialty. Two countries have a special training program for pediatric allergists. Passing a board examination is mandatory in 3 countries, and recertification every 2 to 5 years occurs without examination. Sublingual and subcutaneous SITs are available in all Latin American countries. No legislation restricts SIT prescription and it can be performed by nonspecialists in 7 of 11 countries. In 90% of countries, allergists use allergen extracts from the United States (subcutaneous immunotherapy) and Europe (sublingual and subcutaneous immunotherapies), and 50% also manufacture extracts locally. Only 1 country has legal requirements for the quality of raw materials.  CONCLUSION:  The present analysis helps to identify gaps in the field of allergologic training and SIT in Latin America, many of them amendable.","palabras_clave":"INMUNOTERAPIA|EPIDEMIOLOGÍA|EDUCACIÓN|LATINOAMERICA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64740,"idioma_id":4,"titulo":"Angiotensin II AT1 receptors are involved in neuronal activation induced by amphetamine in a two-injection protocol","resumen":"It was already found that Ang II AT1 receptors are involved in the neuroadaptative changes induced by a single exposure to amphetamine, and such changes are related to the development of behavioral and neurochemical sensitization. The induction of the immediately early gene c-fos has been used to define brain activated areas by amphetamine. Our aim was to evaluate the participation of AT1 receptors in the neuronal activation induced by amphetamine sensitization. The study examined the c-fos expression in mesocorticolimbic areas induced by amphetamine challenge (0.5mg\/kg i.p) in animals pretreated with candesartan, a selective AT1 receptor blocker (3mg\/kg p.o × 5 days), and amphetamine (5mg\/kg i.p) 3 weeks before the challenge. Increased c-fos immunoreactivity was found in response to the amphetamine challenge in the dorsomedial caudate-putamen and nucleus accumbens, and both responses were blunted by the AT1 receptor blocker pretreatment. In the infralimbic prefrontal cortex, increased c-fos immunoreactivity was found in response to amphetamine and saline challenge, and both were prevented by the AT1 receptor blocker.No differences were found neither in ventral tegmental area nor prelimbic cortex between groups.Our results indicate an important role for brain Ang II in the behavioral and neuronal sensitization induced by amphetamine.","palabras_clave":"ANGIOTENSIN|AMPHETAMINE|SENSITIZATION|C-FOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64741,"idioma_id":4,"titulo":"The oxidative stress induced in vivo by Shiga toxin-2 contributes to the pathogenicity of haemolytic uraemic syndrome","resumen":"Typical haemolytic uraemic syndrome (HUS) is caused by Shiga toxin (Stx)-  producing Escherichia coli infections and is characterized by thrombotic  microangiopathy that leads to haemolytic anaemia, thrombocytopenia and  acute renal failure. Renal or neurological sequelae are consequences of irreversible tissue damage during the acute phase. Stx toxicity and the acute inflammatory response raised by the host determine the development of HUS. At present there is no specific therapy to control Stx damage. The pathogenic role of reactive oxygen species (ROS) on endothelial injury has been largely documented. In this study, we investigated the in-vivo effects of Stx on the oxidative balance and its contribution to the development of HUS in mice. In addition, we analysed the effect of anti-oxidant agents as therapeutic tools to counteract Stx toxicity.We demonstrated that Stx induced an oxidative imbalance, evidenced by renal glutathione depletion and increased lipid membrane peroxidation. The increased ROS production by neutrophils may be one of the major sources of oxidative stress during Stx intoxication. All these parameters were ameliorated by anti-oxidants reducing platelet activation, renal damage and increasing survival. To conclude, Stx generates a pro-oxidative state that contributes to kidney failure, and exogenous antioxidants could be beneficial to counteract this pathogenic pathway.","palabras_clave":"hus|Stx|Antioxidantes|raton"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64742,"idioma_id":4,"titulo":"Immunogenicity of Hypothetical Highly Conserved Proteins as Novel Antigens in Anaplasma marginale","resumen":"Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of &#946;-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-&#945; and IFN-&#947; transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.","palabras_clave":"ANAPLASMA|MARGINALE|PROTEINS|IMMUNOGENICITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64743,"idioma_id":4,"titulo":"The Notch Signaling Pathway: its Role in Focal CNS Demyelination and Apotransferrin-Induced Remyelination.","resumen":"ABSTRACT   Oligodendroglial damage and demyelination are common pathological features characterizing white matter and neurodegenerative disorders. Identifying the signaling pathways involved in myelin repair through oligodendroglial progenitor maturation is essential for the development of new therapies. This paper investigated the role of the Notch signaling pathway in CNS demyelination and apotransferrin-induced remyelination in a focal lysolecithin-induced demyelination model in rats. Notch was found activated in Nestin-expressing neural progenitor cells and in NG2-expressing oligodendroglial precursor cells in the subventricular zone and corpus callosum of lysolecithin-demyelinated rats. Notch activation seemed to be driven by Jagged1, which led to a high expression of downstream gene Hes5 in the subventricular zone of demyelinated rats.  Apotransferrin injection induced remyelination, while the injection of the &#947;-secretase inhibitor reversed this effect.  In addition, 24 h after apotransferrin injection, evidence showed Notch activation concomitantly with an increase in F3\/contactin levels and the upregulation of the myelin-associated glycoprotein gene in the subventricular zone and corpus callosum of demyelinated rats. Collected evidence supports the participation of both canonical and non-canonical Notch signaling pathways in demyelination\/remyelination. Notch activation was found to trigger Hes5 expression as a consequence of focal demyelination, which might promote oligodendroglial precursor cell proliferation.  During apotransferrin-induced remyelination, Notch activation seemed to be mediated by the expression of F3\/contactin, which might induce apotransferrin-mediated oligodendroglial maturation.","palabras_clave":"APOTRANSFERRIN|DEMYELINATION|NOTCH SIGNALING|REMYELINATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64744,"idioma_id":4,"titulo":"Notch signaling in the pathologic adult brain","resumen":"Along the entire lifetime, Notch is actively involved in dynamic changes in the cellular architecture and function of the nervous system. It controls neurogenesis, the growth of axons and dendrites, synaptic plasticity, and ultimately neuronal death. The specific roles of Notch in adult brain plasticity and neurological disorders have begun to be unraveled in recent years, and pieces of experimental evidence suggest that Notch is operative in diverse brain pathologies including tumorigenesis, stroke, and neurological disorders such as Alzheimer?s disease, Down syndrome, and multiple sclerosis. In this review, we will cover the recent findings of Notch signaling and neural dysfunction in adult human brain and discuss its relevance in the pathogenesis of diseases of the central nervous system.","palabras_clave":"alzheimer|brain tumors|down symdrome|neurodegeneration"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64745,"idioma_id":4,"titulo":"The most complete and detailed X-ray view of the SNR Puppis A","resumen":"Aims. With the purpose of producing the first detailed full view of Puppis A in X-rays, we carried out new XMM-Newton observations covering the missing regions in the southern half of the supernova remnant (SNR) and combined them with existing XMM-Newton and Chandra data.             Methods. Two pointings toward the south and southwest of Puppis A were observed with XMM-Newton. We combined these data with archival XMM-Newton and Chandra data and produced images in the 0.3-0.7, 0.7-1.0, and 1.0-8.0 energy bands.             Results. We present the first sensitive complete X-ray   image of Puppis A. We investigated its morphology in detail, carried   out a multiwavelength analysis, and estimated the flux density and   luminosity of the whole SNR. The complex structure observed across the   remnant confirms that Puppis A evolves in an inhomogeneous, probably   knotty interstellar medium. The southwestern corner includes filaments   that perfectly correlate with radio features suggested to be associated   with shock\/cloud interaction. In the northern half of Puppis A the   comparison with Spitzer infrared images shows an excellent correspondence between X-rays and 24 and 70 &#956;m emission features, while to the south there are some matched and other unmatched features. X-ray flux densities of 12.6&nbsp;×&nbsp;10-9,6.2&nbsp;×&nbsp;10-9, and 2.8&nbsp;×&nbsp;10-9 erg cm-2 s-1   were derived for the 0.3&#8722;0.7, 0.7&#8722;1.0, and 1.0&#8722;8.0 keV bands,   respectively. At the assumed distance of 2.2 kpc, the total X-ray   luminosity between 0.3 and 8.0 keV is 1.2 ×&nbsp;1037 erg s-1. We also collected and updated the broad-band data of Puppis A between radio and GeV &#947;-ray   range, producing its spectral energy distribution. To provide   constraints to the high-energy emission models, we re-analyzed radio   data, estimating the energy content in accelerated particles to be Umin&nbsp;=&nbsp;4.8&nbsp;×&nbsp;1049 erg and the magnetic field strength B&nbsp;~&nbsp;26 &#956;G. ","palabras_clave":"SUPERNOVA REMNANTS|X-RAYS|PUPPIS A"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64746,"idioma_id":4,"titulo":"How important are internal temperature gradients in French straws during freezing of bovine sperm in nitrogen vapor?","resumen":"The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen\/extender packaged in straw was extended to predict internal temperature gradients during the cooling\/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen\/extender liquid column was 1.5 ºC for an external heat transfer coefficient, h = 15 W\/(m2 K), and only 0.5 ºC for h = 5 W\/(m2 K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoa","palabras_clave":"Freezing |bovine sperm  |heat transfer coefficient  temperature gradients  |nitrogen vapor | finite element method |French plastic straws"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64747,"idioma_id":4,"titulo":"The role of the N-terminal tail for the oligomerization, folding and stability of human frataxin","resumen":"The N-terminal stretch of human frataxin (hFXN) intermediate (residues 42-80) is not conserved throughout evolution and, under defined experimental conditions it behaves as a random-coil. However, overexpression of hFXN56-210 in E. coli yields a multimer, whereas hFXN81-210, the mature form of hFXN, is monomeric. Thus, cumulative experimental evidence points to the N-terminal moiety as an essential element for the assembly of a high molecular weight oligomer. In this context, we explore the conformation and stability of hFXN56-210. The biophysical characterization by fluorescence, CD and SEC FPLC shows that subunits are well-folded, sharing similar stability to hFXN90-210. On the other hand, controlled proteolysis indicates that the N-terminal stretch is labile in the context of the multimer, whereas the FXN domain remains strongly resistant (residues 81-210). In addition, GdmCl at low concentration disrupts intermolecular interactions, shifting the ensemble toward the monomeric form. Furthermore, peptide 56-81, putatively responsible for promoting protein-protein interactions, was studied in its propensity to acquire ordered secondary structure by virtue of TFE or SDS addition. Depending on the environment, this peptide adopts &#945;-helical or &#946;-strand structure. Complementary, the analysis of mutual information along hFXN1-210 reveals three independent information networks. Interestingly, one of them involves residues in the 50-70 region, suggesting a common co-evolutionary relationship. The conformational plasticity of the N-terminal tail might impart hFXN the ability to act as a recognition signal as well as an oligomerization trigger. Understanding the fine tuning of these activities and the resulting balance will bear direct relevance for ultimately comprehending hFXN function.","palabras_clave":"Frataxin|stability|folding| intermolecular interactions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64748,"idioma_id":4,"titulo":"Characterization and emulsifying properties of different sunflower phosphatidylcholine enriched fractions","resumen":"Fractions enriched in specific phospholipids are desirable for different industrial purposes because of their characteristic physicochemical and functional properties. We studied the fractionation process of &nbsp;sunflower lecithin using different absolute?]ethanol:water ratios and pHs and then evaluated the &nbsp;emulsifying properties of phosphatidyl?]choline?](PC?]) enriched fractions in oil:water 30:70 v\/v mixtures. We observed a high recovery of PC and a low content of phosphatidyl inositol in all the PC?]enriched &nbsp;fractions thus obtained along with the highest yields of this phospholipid after extraction with an absolute?] ethanol:water mixture of 96:4 v\/v. The use of different pH values for the different solvent extraction media &nbsp;did not markedly modify the yield of the enriched fractions. The extraction coefficients for PC and phosphatidyl ethanolamine (PE) evidenced an increase in both these phospholipids in the PC?]enriched &nbsp;fractions upon extraction with the higher concentration of absolute ethanol. Emulsions containing the PC?]enriched fractions obtained with the absolute?]ethanol:water mixture of 96:4 v\/v exhibited the highest stability at pH 7.5 because of the high PC\/PE ratio compared to that of the PC?]enriched fractions &nbsp;extracted with the absolute?]ethanol:water mixture of 90:10 v\/v. A high emulsifier concentration resulted &nbsp;in a low mean D [4,3] diameter of the particles and a high stability. ","palabras_clave":"emulsifying properties |sunflower phosphatidylcholine enriched fractions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64749,"idioma_id":4,"titulo":"Emulsifier and antioxidant properties of by-products obtained by enzymatic degumming of soybean oil","resumen":"The enzymes used in degumming, called phospholipases, specifically act on phospholipids without degrading the oil itself. Degumming using a phospholipase C enzyme allows to meet all market specifications while it increases the oil yield. The aim of this study was to evaluate antioxidant and emulsifier properties of the recovered gum (RG) obtained by enzymatic oil degumming process of crude soybean oil subjected to modifications as deoiling (RG deoiled) or ethanol fractionation (RG soluble and insoluble). RG soluble allowed obtaining more stable O\/W emulsions (30:70 w\/w) in comparison with those by-products assayed at different concentrations (0.1?1.0%). Also, deoiled soybean lecithin (DSL) andRG deoiled had a similar behavior in relation to the kinetic destabilization (%BS profiles), despite the different degumming processes used to obtain these samples. The study on induction times (Metrohm Rancimat) showed a significant antioxidant effect (p<0.05) against a refined sunflower oil associated with all the by-products analyzed. However, RG soluble and DSL showed a strong effect on the oil stability at high concentrations (1000?2000 ppm). These results showed that the deoiled recovered gum and its derivates obtained by ethanol fractionation are a potential alternative for industrial application as additive.","palabras_clave":"31P NMR|Enzymatic oil degumming|Fractionation process|Phospholipids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64750,"idioma_id":4,"titulo":"Tolerance induction at the early maternal-placental interface through selective cell recruitment and targeting by immune polypeptides","resumen":"Pregnancy challenges immune cells and immunomodulatory circuits of the mother and the developing fetus to dynamically adapt to each other in an homeostatic and tolerant environment for fetal growth. This entails the coordination of multiple cellular processes all devoted to accommodate and nourish the fetus while protecting the mother from endogenous and exogenous threatens. From the earliest stages of pregnancy, several strategies to efficiently communicate immune and trophoblast cells within the interface or at a distance were identified and chemokines might act at on different targets through direct or indirect mechanisms. Here, we briefly review some mechanisms of T regulatory cell recruitment to the early maternal-placental interfaces to accomplish immunotolerance and homeostatic control and we discuss evidence on two locally released polypeptides, RANTES (regulated on activation, normal, T-cell expressed, and secreted) and vasoactive intestinal peptide (VIP), as novel contributors to the multiplicity of immune tolerant responses and uterine quiescence requirements.","palabras_clave":"VIP|TOLERANCE|MATERNAL-PLACENTAL INTERFACE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64751,"idioma_id":4,"titulo":"Latitudinal variation in starvation resistance is explained by lipid content in natural populations of Drosophila melanogaster","resumen":"One of the most common environmental stressors is a shortage or   suboptimal quality of food, thus all animals deal with periods of   starvation. In the present study we examine variation in starvation   resistance, longevity and body lipid content and the correlations   between traits along an environmental gradient using isofemale lines   recently derived from natural populations of Drosophila melanogaster   from South America. The use of isofemale lines and controlled rearing   laboratory conditions allows us to investigate within and among   population components of genetic variation and the potential   associations among starvation resistance, longevity and body lipid   content. All these traits were analyzed separately in females and males,   improving our understanding of sexual dimorphism. Our results revealed   significant differences among populations in starvation resistance and   longevity. Actually, the opposing latitudinal cline detected for   starvation resistance suggests that natural selection played an   essential role in shaping the pattern of geographic variation in this   trait. Moreover, we also detected a positive relationship between   starvation resistance and body lipid content in both sexes, providing   evidence for a physiological and\/or evolutionary association between   these traits. Conversely, starvation resistance was not correlated with   longevity indicating that these traits might be enabled to evolve   independently. Finally, our study reveals that there is abundant within   population genetic variation for all traits that may be maintained by   sex-specific effects.","palabras_clave":"GENETIC CORRELATION|LATITUDINAL CLINE|GENOTYPE BY SEX INTERACTION|DROSOPHILA MELANOGASTER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64752,"idioma_id":4,"titulo":"New insights into thyroglobulin gene: Molecular analysis of seven novel mutations associated with goiter and hypothyroidism","resumen":"New  insights  into  thyroglobulin  gene:  molecular  analysis  of  seven  novel  mutations  ssociated  with  goiter  and  hypothyroidism","palabras_clave":"THYROGLOBULIN|MOLECULAR ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64753,"idioma_id":4,"titulo":"Production of Pectinolytic Enzymes by the Yeast Wickerhanomyces anomalus Isolated from Citrus Fruits Peels","resumen":"Wickerhamomyces anomalus is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the  present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were  determined in order to evaluate the application of the supernatant in the maceration of potato tissues. W. anomalus was able to  produce PGin liquid mediumcontaining glucose and citrus pectin,whosemode of actionwas mainly of endo type.The supernatant  did not exhibit esterase or lyase activity. No others enzymes, capable of hydrolyzing cell wall polymers, such as cellulases and  xylanases,were detected. PGshowedmaximal activity atpH4.5 and at temperature range between 40&#8728;Cand50&#8728;C. It was stable in the  pH range from3.0 to 6.0 and up to 50&#8728;Cat optimumpH.The enzymatic extractmacerated potato tissues efficiently.Volume of single  cells increased with the agitation speed.The results observed make the enzymatic extract produced by W. anomalus appropriate for  future application in food industry, mainly for the production of fruit nectars or mashed of vegetables such as potato or cassava, of  regional interest in the province of Misiones, Argentine.","palabras_clave":"Pectinolytic Enzymes|Wickerhanomyces anomalus|Citrus Fruits Peels"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64754,"idioma_id":5,"titulo":"REVISION DE VELOCIDADES DE VIENTO EXTREMAS EN TRES LOCALIDADES DEL SUR DE ARGENTINA","resumen":"La valoración de la acción del viento sobre un edificio enla etapa de diseño requiere conocer la magnitud de las máximas velocidades deviento a las que estará expuesto el edificio durante su vida útil. En lapráctica profesional, esta información se extrae del llamado mapa de vientos,que es parte de los reglamentos nacionales de acción del viento sobreestructuras civiles. En Argentina, el reglamento pertinente es el CIRSOC 102.Si bien fue editado en 2005, el mapa de vientos que trae este reglamento sebasa principalmente en información meteorológica del período entre 1970 y 1990.En este trabajo se discute la metodología usada para construir este mapa, a laluz de procedimientos más actualizados desarrollados en los últimos 20 años. Seilustra la discusión aplicando algunos de estos nuevos procedimientos aregistros de tres estaciones meteorológicas de zonas donde el clima estádominado por depresiones y se muestra la conveniencia de actualizar el mapa.","palabras_clave":"viento|velocidades extremas|codificación"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64755,"idioma_id":4,"titulo":"Nectar ecology of Datura ferox (Solanaceae): an invasive weed with nocturnal flowers in agro-ecosystems from central Argentina.","resumen":"Abstract Plant\u2013pollinator interactions provide highlyimportant ecological functions, and are influenced by floralnectar characteristics. The night blooming Datura ferox isan excellent model to test general hypotheses on the relationshipbetween nectar traits (e.g., nectar secretion patterns,nectar chemical composition), pollinators andreproductive success for invasive, weedy species in highlymodified ecosystems as crop fields. We hypothesized anadjustment between nectar composition and secretiondynamics through flower anthesis and the activity andrequirements of nocturnal pollinators. Nectar chemicalanalyses showed low quantities of amino acids and lipids,phenolics, and alkaloids were not detected. D. feroxshowed sucrose-dominant nectar with comparable amountof hexoses. Sugar proportions did not vary between populationsor during flowering season. Most nectar is secretedbefore flower opening. Nectar resorption was detected atthe end of anthesis. Experimentally drained flowers of bothpopulations increased nectar production up to 50 % in thetotal amount of sugar per flower compared to controlflowers. Nectar standing crop was relatively constant duringthe flowering season, but differences were detectedbetween populations. Nectar traits of D. ferox would befavoring cross-pollination and maintaining seed productionof this weed, since recently open flowers display a higheramount of nectar and they can renew nectar after a pollinatorvisit or reabsorb it at the end of anthesis. This nectarsource may be important for native pollinators consideringthat human-induced forest fragmentation is related with theimpoverishment of native flora from agro-ecosystems.","palabras_clave":"NECTAR PRODUCTION|NECTAR SUGAR COMPOSITION|HAWKMOTH POLLINATION|WEED REPRODUCTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64756,"idioma_id":4,"titulo":"Changes in forest structure and tree recruitment in Argentinean Chaco: Effects of fragment size and landscape forest cover","resumen":"Subtropical Argentinean Chaco forests have been severely deforested and fragmented due to agricultureduring the last six decades. The most affected forests are located in areas that are most favorable forcrops. This is the case of the semi-deciduous ??bosque de tres quebrachos?? (??three quebrachos forest??)in southwest of Chaco Province (Argentina), where this study was carried out. The aim of this workwas to analyze the fragmentation process, considering spatial (fragments and landscape levels) and temporal(land use changes) scales, studying the effects on the forest structure (density and basal area) andtree recruitment. All trees (adults and saplings) were recorded and measured in 112 plots (400 m2) distributedin 28 forest fragments (0.9?160 ha) located at four different landscape samples (4500 ha) in oneenvironmentally homogeneous zone (72,804 ha). The four landscape samples have different current forestcover (low\/high) and different land use history (low\/high). Using linear mixed models, we evaluatedthe effects of current and historic fragment size, landscape forest cover and land use changes on the densityand basal area at stand level, and considering the two different strata (upper and middle). Resultsshowed that fragmentation did not severely affect the structure of the older age classes of trees in theremaining fragments but affect the tree recruitment, which could influence over the ability of the forestto perpetuate itself. We found positive relationships between sapling density and fragment size for thetree species of middle stratum, and between sapling density and forest cover at landscape level for thespecies of upper stratum. Regarding adults, we found a negative relationship between density and historicfragment size, probably related to the past timber harvesting. We concluded that even small fragmentsof three quebrachos forest and those located in highly deforested landscapes have highconservation value since their structure does not differ from that of the larger fragments or landscapeswith higher forest cover. Our results reveal the importance of considering landscape and fragment scalessimultaneously to better understand the fragmentation process and improve the recommendations forthe management of fragmented landscapes.","palabras_clave":"fragmnetación|agroecosistemas|bosque chaqueño|regeneración"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64757,"idioma_id":4,"titulo":"Linking mycorrhizal fungi and soil nutrients to vegetative and reproductive ruderal plant development in a fragmented forest at central Argentina","resumen":"We studied the actual links between mycorrhizal fungi, nutrient availability and plant development inseveral sites of fragmented Chaco forests. Specifically, we evaluated whether arbuscular mycorrhizalfungi (AMF) and dark septate endophytes (DSE) colonization and availability of soil nutrients are relatedto growth (biomass and plant height) and reproductive traits (pollen size and pollen and fruit production)of two ruderal, annual, congeneric Euphorbia species along a gradient of forest fragmentation. Weassessed relationships between forest fragment size and isolation, nutrient availability, mycorrhizal fungalvariables and plant development at eleven Chaquean forest fragments (0.5?1000 ha) immersed in anagricultural landscape in central Argentina. Mycorrhizal colonization was not related to forest fragmentisolation, positively related to forest fragment size and negatively related to soil nutrient availability inboth hosts along the fragmentation gradient. Plant reproductive and vegetative traits were related tomycorrhizal colonization. In general, plant height, biomass, and fruit production were negatively relatedto AMF, and pollen production was positively related to DSE, in both ruderal hosts (native and exotic). Wefound remarkable relationships along a forest fragmentation gradient that reliably link forest fragmentsize to lower nutrient availability and increased mycorrhizal colonization in native\/exotic ruderal hosts,while negatively linking AMF colonization to plant development and reproductive success.","palabras_clave":"plant reproduction|fungi|soil nutrients|ruderal plants"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64759,"idioma_id":4,"titulo":"Pollination biology of Pseudostifftia kingii H.Rob. (Asteraceae), a rare endemic Brazilian species with uniflorous capitula.","resumen":"Abstract (The pollination biology of Pseudostifftia kingiiH.Rob. (Asteraceae), a rare endemic Brazilian species withuniflorous capitula). P. kingii is a monotypic genus withinthe Asteraceae family that grows under extreme environmentalconditions and possesses an unusual single-floweredcapitulum. It is endemic to Chapada Diamantina,Bahia, Brazil, a region dominated by a mosaic of rocky,sandy flats and bogs in the upper elevational zone (above900?1,000 m). To gain a better understanding of the pollinationbiology of P. kingii, we examined its floraldevelopment, floral rewards, reproductive strategies, andfloral visitors. The flowers exhibit asynchronous diurnalanthesis with three distinct floral stages, each differing withregard to male?female maturation and nectar availability;these floral stages appear to influence the activity of theflower visitors. Although several floral characteristics of P.kingii suggest that it is an entomophilous species, observationsrevealed it as a generalist species for which hummingbirdsand bees are the main floral visitors, differingslightly in the diurnal period of their visits. P. kingii canreproduce through xenogamy, geitonogamy, and whatappears to be allogamy, but with different sizes of fruits(achenes) produced depending on the reproductive strategyemployed. This study revealed some important features ofpollination and floral biology within Asteraceae and providesclues for pollen dispersion in a harsh environment.","palabras_clave":"biología reproductiva|Asteraceae"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64760,"idioma_id":5,"titulo":"Enseñanza de la Diversidad Vegetal en la Universidad: evaluación de los alumnos a una nueva propuesta didáctica basada en la problematización del conocimiento","resumen":"Se presenta la evaluación de una innovación didáctica implementada en la asignatura Diversidad Vegetal II (Carrera de Ciencias Biológicas). En la nueva propuesta se modificó la metodología de enseñanza, así como la selección y organización de los contenidos, cambiando de un sistema de transmisión-recepción de conocimiento a uno de problematización del mismo. Aquí se evaluó cada propuesta en general y el logro de los distintos objetivos en particular, a través de la opinión de los alumnos que cursaron la asignatura con ambas modalidades pedagógicas. La mayoría de los estudiantes consideró que la propuesta basada en la resolución de problemas fue mejor, puesto que adquirieron las capacidades esperadas, les permitió la discusión de conceptos y el análisis crítico de la información e ideas teóricas y metodológicas, poder participar en la búsqueda y síntesis de la información, y adquirir mayor juicio crítico. Los alumnos reconocen que esta nueva metodología les permitió gestionar por sí mismos el conocimiento sobre la diversidad vegetal.","palabras_clave":"diversidad vegetal|evaluación de la metodología de enseñanza|aprendizaje basado en la resolución de problemas|enseñanza y aprendizaje por investigación"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64761,"idioma_id":5,"titulo":"En defensa de la causa aliada. La militancia de Alberto Gerchunoff durante la Primera Guerra Mundial","resumen":"Este artículo se propone abordar las actividades desplegadas por Alberto Gerchunoff en favor de la causa aliada, en el marco de las polémicas suscitadas por la posición del gobierno argentino frente a esa primera conflagración mundial. Tras reseñar brevemente las peculiaridades de esa coyuntura política, nos adentraremos en el análisis de las intervenciones públicas del autor y de los postulados ideológicos en los que estas se sustentaron.&nbsp;&nbsp; &nbsp;","palabras_clave":"PRIMERA GUERRA MUNDIAL|ALBERTO GERCHUNOFF|INTELECTUALES|ALIADOFILOS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64762,"idioma_id":5,"titulo":"RASGOS ESTRUCTURALES EN LA SIERRA PAMPEANA DE VELASCO Y ALREDEDORES A PARTIR DE LOS CAMPOS GRAVIMÉTRICOS Y MAGNETOMÉTRICOS","resumen":"La región que comprende la sierra de Velasco y el Sistema de Famatina, en el centro-oeste de la Provincia de La Rioja - Argentina, es un área clave para el estudio de la amalgamación de terrenos y tectónica vinculada con el levantamiento andino. En éste trabajo, se definen algunos rasgos de la estructura cortical de ésta región. Para ello, mediante la aplicación de métodos potenciales(gravedad y magnetismo), adecuadamente procesados e interpretados a través de técnicas semiautomáticas y de resalto de anomalías, se logró determinar: a) una región que respondería a la zona de sutura entre los terrenos Famatina y Pampia y b) fallas relacionadas al levantamiento de las sierras de Famatina y de Velasco. Los resultados anteriores se representan en una sección este - oeste en un modelo estructural de bloques de basamento entre Famatina y Velasco, hasta 12 km de profundidad. En él señalamos además la distribución de las fallas y sus buzamientos.","palabras_clave":"Gravity|magnetism|Werner deconvolution|suture|analytic signal"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64763,"idioma_id":4,"titulo":"OXYGEN TENSION MODULATES AQP9 EXPRESSION IN HUMAN PLACENTA","resumen":"Placental hypoxia has been implicated in pregnancy pathologies such as preeclampsia. We have previously reported that AQP9 is highly expressed in syncyciotrophoblast from normal placentas and shows an overexpression in preeclamptic placentas, with a lack of functionality for water transport. Up to now, the response of AQP9 to changes in the oxygen tension in trophoblast cells is still unknown.        Objective: Our aim was to establish whether alterations in oxygen levels may modulate AQP9 expression in human placenta.        Methods: A theoretical analysis of the human AQP9 gene to find conserved DNA regions that could serve as putative HIF-1 binding sites. Then, explants from normal placentas were cultured at different concentrations of oxygen or with 250 &#956;M CoCl2. AQP9 molecular expression and water uptake was determined.        Results: Fourteen consensus HIF-1 binding sites were found in the human AQP9 gene, but none of them in the promoter region. However, placental AQP9 decreased abruptly when HIF-1&#945; is expressed by deprivation of oxygen or CoCl2 stabilization. In contrast, after reoxygenation, HIF-1&#945; was  undetectable while AQP9 increased significantly and changed its cellular distribution, showing the same pattern as that previously described in preeclamptic placentas. Accordingly with the decrease in AQP9 expression, water uptake decreased in explants exposed to hypoxia or treated with CoCl2. Conversely as we expected, after reoxygenation, water uptake  decreased dramatically compared to the control and was not sensitive to HgCl2.         Conclusion: Our findings suggest that oxygen tension may modulate AQP9 expression in human placenta. However, the role of AQP9 still remains uncertain.","palabras_clave":"hypoxia|AQP9|placenta|preeclampsia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64764,"idioma_id":4,"titulo":"Impaired mammary gland T cell population during early lactation in hypoprolactinemic lactation deficient rats","resumen":"*J P Mackern-Oberti and S R Valdez contributed equally to the experimental design, analysis and writing of this work.Mammary stroma is composed of various cell types, among them migratory leukocytes. Although mammary antibody secreting cells have been extensively studied, reports focusing on mammary T cells are scarce. It is thought that the recruitment mechanism of leukocytes to the mammary gland (MG) are controlled by pregnancy- and lactation-specific stimuli. But whether PRL modulates the T cell population in MG is still unknown. Our aim was to study the relationship between prolactin (PRL) levels and T and B cells in early lactation (L2, day 2 postpartum) and mid lactation (L12). In order to investigate if PRL is associated to homing events to MG, female Sprague Dawley (SD) and SD derived desmoglein 4-\/- hairless (phenotype with lactation deficit, OFA hr\/hr) rats were sacrificed at estrus, pregnancy and postpartum, and blood, MG and corpora lutea were obtained to perform FACS, Real Time PCR, histological and RIA studies. Serum PRL levels were lower in OFA hr\/hr than in SD at early lactation. MG of OFA hr\/hr rats showed less secretory material compared to SD. FACS analysis showed lower percentage of MG CD3+ cells in OFA hr\/hr compared to SD rats on L2 and L12. OFA hr\/hr rats showed higher absolute numbers of circulating CD3+ cells compared to SD on L2 but not on L12. These results show that MG T cell population is affected in early lactating OFA hr\/hr rats and strongly suggest that serum PRL levels may be involved in mammary homing T cell events, probably helping antibody secreting cells and protecting the gland during lactation development.","palabras_clave":"PROLACTIN|T-CELLS|MAMMARY-GLAND"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64765,"idioma_id":4,"titulo":"Effects of thyroid status on NEI concentration in specific brain areas related to reproduction during the estrous cycle","resumen":"Abstract: We previously showed that short-term hypo- and hyperthyroidism induce changes in neuropeptide glutamic-acid-isoleucine amide (NEI) concentrations in discrete brain areas in male rats. To investigate the possible effects of hypo- and hyperthyroidism on NEI concentrations mainly in hypothalamic areas related to reproduction and behavior, femalerats were sacrificed at different days of the estrous cycle. Circulating luteinizing hormone (LH), estradiol and progesterone concentrations were measured in control, hypothyroid (hypoT, treated with PTU during 7-9 days) and hyperthyroid (hyperT, L-T4 during 4-7 days) animals. Both treatments blunted the LH surge. Hypo- and hyperthyroidism increased estradiol concentrations during proestrus afternoon (P-PM), although hypoT rats showed lower values compared to control during proestrus morning (P-AM). Progesterone levels were higher in all groups at P-PM and in the hyperT during diestrus morning (D2). NEI concentrations were lower in hypoT rats during the estrous cycle except in estrus (E) in thepeduncular part of the lateral hypothalamus (PLH). They were also reduced by both treatments in the perifornical part of the lateral hypothalamus(PeFLH) during P-PM. Hypothyroidism led to higher NEI concentrations during P-PM in the organum vasculosum of the lamina terminalis and anteroventral periventricular nucleus (OVLT + AVPV). The present results indicate that NEI concentration is regulated in a complex manner by hypoand hyperthyroidism in the different areas studied, suggesting a correlation between NEI values and the variations of gonadal steroid levels during estrous cycle. These changes could be, in part, responsible for the alterations observed in the hypothalamic-pituitary-gonadal axis in these pathologies.","palabras_clave":"Neuropeptide glutamic-acid-isoleucine-amide (NEI)|hypothalamus|hypothyroidism   |hyperthyroidism|estrous cycle|reproduction"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64766,"idioma_id":4,"titulo":"Lipoprotein lipase expression in unmutated CLL patients is the consequence of a demethylation process induced by the microenvironment","resumen":"The role that abnormal LPL expression could have in disease evolution, has been also addressed by previous work demonstrating that lipase-associated genes and triglyceride-specific lipase activity were increased when comparing CLL B cells to normal CD5+ B cells. Our results showing proliferation of the tumoral clone associated with demethylationand subsequent LPL expression support these results and highlight the idea that LPL gene could constitute a potential therapeutic target in Um CLL cases. By comparing methylation changes in the LPL-CpG island between Um and Mut CLL patients, we demonstrate a clearassociation between LPL expression and a demethylation process in the CpG island of the LPL gene. This process can be induced in the leukemic clone by specific microenvironment signals, delivered by CD40L\/IL-4 and anti-IgM, but not by T-independent related signalsdelivered through Toll-like receptors. Overall, these results suggest that an epigenetic mechanism, triggered by the microenvironment, regulates LPL expression in CLL B cells.","palabras_clave":"LEUKEMIA|LPL|MICROENVIRONMENT|PROGNOSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64767,"idioma_id":4,"titulo":"Milk molars or extra premolars in Mesotheriinae (Mesotheriidae, Notoungulata): new insights into an old controversy","resumen":"A recently recovered specimen of Mesotheriinae (Mesotheriidae, Notoungulata) from the late Miocene?early Pliocene of La Rioja Province (Argentina), CRILAR Pv 433, corresponds to an individual with three upper premolars, which appears to be an ?anomaly? among mesotheriines. The detailed study of this specimen, however, brings up an old controversy on the interpretation of different mesotheriine specimens with three upper or two lower premolars. After being described as different taxa, these were later considered to be juvenile representatives of other known species; the three upper or two lower teeth were interpreted as the milk molars DP2?4 and dp3?4, respectively, which would be replaced in adult life just for two upper (P3?4) and one lower (p4) permanent premolars. The new material leads us to set up a different interpretation. In our opinion, all these specimens actually preserve the permanent dentition, corresponding to different ontogenetic stages of more or less young individuals. This consideration implies the necessity of a deep systematic revision of the whole subfamily keeping in mind this new point of view and the ontogenetic variation within a species. Therefore, the presence of P2\/p3 is not enough to define a different taxon at this moment. Pending this taxonomic revision, the dental morphology of CRILAR Pv 433 resembles both Typotheriopsis (e.g., upper premolars with one labial sulcus) and Pseudotypotherium (e.g., P4 with lingual groove, wide median lobe of M3) as these two late Miocene genera are characterized at present. Furthermore, P2\/p3 could be expelled soon in the life time of individuals, and even the presence of P2\/p3 could be a variable character within a same taxon; if so, this might reflect an evolutionary trend to the loss of a dental element within mesotheriines, but the revision of a large sample is necessary to support or reject these hypotheses. ","palabras_clave":"MESOTHERIIDAE|NOTOUNGULATA|DENTITION|ONTOGENY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64768,"idioma_id":4,"titulo":"Identification, cloning and expression of an insecticide cry8 gene from Bacillus thuringiensis INTA Fr7-4","resumen":"Insecticidal activity of B. thuringiensis is attributed largely to the crystal proteins.  These were found with specific toxic activity against insects in different  orders. A novel cry8 gene from Bacillus thuringiensis strain INTA Fr7-4 from Argentina was  characterized. The encoded protein, Cry8Qa2, is 1184 amino acids long and its  sequence is more similar to those of Cry8F class. We cloned and expressed the  protein in an acrystalliferous strain of B.  thuringiensis using two differentially regulated promoters. The recombinant  strains produced ovoid crystals with low toxicity against larvae of Anticarsia gemmatalis (Lepidoptera: Noctuidae).  The morphology and insecticidal properties of these crystals resembled those produced  by the native strain INTA Fr7-4.","palabras_clave":"Bacillus thuringiensis|cry8|expression"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64769,"idioma_id":4,"titulo":"A CsrA\/RsmA translational regulator gene encoded in the replication region of a Sinorhizobium meliloti cryptic plasmid complements Pseudomonas fluorescens rsmA\/E mutants","resumen":"Members of the CsrA\/RsmA family are global regulatory proteins that bind to mRNAs, usually atthe ribosome-binding site, to control mRNA translation and stability. Their activity is counteractedby small non-coding RNAs (sRNAs), which offer several binding sites to compete with mRNAbinding. The csrA\/rsmA genes are widespread in prokaryotic chromosomes, although certainphylogenetic groups such as Alphaproteobacteria lack this type of global regulator. Interestingly, acsrA\/rsmA-like sequence was identified in the replication region of plasmid pMBA19a from thealphaproteobacterium Sinorhizobium meliloti. This rsmA-like allele (rsmASm) is 58% identical toXanthomonas axonopodis pv. citri chromosomal rsmA and bears an unusual C-terminal extensionthat may fold into an extra a-helix. Homology-based modelling of RsmASm suggests that all keymRNA-binding residues are conserved and correctly positioned in the RNA-binding pocket. Infact, a 1.6 kb fragment from pMBA19a encompassing the rsmASm locus restored rsmA\/Edependentphenotypes of rsmA\/E gacS Pseudomonas fluorescens mutants. The functionality ofRsmASm was confirmed by the gain of control over target aprA9?9lacZ and hcnA9?9lacZtranslational fusions in the same mutant background. The RsmASm activity correlated withWestern blot detection of the polypeptide. Phenotype and translational fusion data from rsmA\/EP. fluorescens mutants expressing RsmX\/Y\/Z RNAs indicated that RsmASm is able to bind theseantagonistic sRNAs. In agreement with the latter observation, it was also found that the sRNARsmY was stabilized by RsmASm. Deletion of the C-terminal extra a-helix of RsmASm affected itscellular concentration, but increased its relative RNA-binding activity. This is believed to be thefirst report of the presence and characterization of a functional csrA\/rsmA homologue in a mobilegenetic element.","palabras_clave":"RsmA|Sinorhizobium meliloti|riboregulation|cryptic plasmid"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64770,"idioma_id":4,"titulo":"Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions","resumen":"The use of antagonistic biological agents, such as fungi and bacteria, offers an economical and safe strategy to manage plant-parasitic nematodes in infested fields. The false root-knot nematode, Nacobbus aberrans, is a damaging parasite of many agronomic and horticultural crops in South and North America. The management of this nematode is challenging and often not profitable for farmers. In greenhouse tests, conducted in Argentina, applications of the strain Pseudomonas protegens CHA0 and its isogenic derivative ARQ1 (used as control) at a rate of 108 cfu ml-1 suppressed infection and reproduction of N. aberrans on tomato roots. However, neither of the strains promoted plant root or shoot growth of the treated plants. Root colonization by the bacteria was assessed by specific PCR-RFLP protocols and fluorescence microscopy. The results obtained in this preliminary study were encouraging and showed the potential of P. protegens CHA0 to be used for the management of the false root-knot nematode on tomato.","palabras_clave":"biological control|Nacobbus aberrans|plant-parasitic nematode|Pseudomonas protegens"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64771,"idioma_id":4,"titulo":"Effects of fire severity on early recovery of Patagonian steppes","resumen":"Fire severity influences the recovery and biodiversity of plant communities. We compared the early post-fire recovery of plant functional groups in two sites (one burnt with high severity and one with low severity) in the northwestern Patagonian steppe.We sampled the post-fire soil seed banks, as well as the composition and cover of aboveground plant species, and calculated richness, diversity and the percentage of exotic species. Low fire severity increased the  diversity of aboveground vegetation and seed banks, whereas high fire severity reduced the cover of all functional groups, with the exception of fugitive species. Tiller production of perennial grasses decreased due to bud bank mortality. In the seed bank, high severity fire reduced fugitive species and increased exotic species. Fugitive species need fire to recruit, and the combination of fire cues and post-fire environmental conditions can explain the high abundance of these species after fire. In Patagonia, the use of fire is not considered a management option because fires are believed to be dangerous to humans, goods and services. However, north-western Patagonia is sparsely populated and the frequent wildfires pose no danger. It is necessary to increase the knowledge of the ecological processes of Patagonian ecosystems related to fire regime and land use, to achieve a reasonable balance between sustainable use and conservation in this region.","palabras_clave":"FUGITIVE SPECIES|FUNCTIONAL GROUPS|SEED BANK|SEMI-ARID GRASSLANDS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64772,"idioma_id":4,"titulo":"Directional convergence of spectral regularization method associated to families of closed operators","resumen":"We consider regularized solutions of linear inverse ill-posed problems obtained withgeneralized Tikhonov-Phillips functionals with penalizers given by linear combinationsof seminorms induced by closed operators. Convergence of the regularized solutions isproved when the vector regularization rule approaches the origin through appropriateradial and diferentiable paths. Characterizations of the limiting solutions are given.Finally, a examples of image restoration using generalized Tikhonov-Phillips methodswith convex combinations of seminorms are shown.","palabras_clave":"INVERSE PROBLEM|REGULARIZATION|TIKHONOV-PHILLIPS|CLOSED OPERATORS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64773,"idioma_id":4,"titulo":"Seasonal variation in genetic population structure of sábalo (Prochilodus lineatus) in the Lower Uruguay River","resumen":"Abstract Prochilodus lineatus is a highly migratory fish  species that sustains the most important commercial fishery  of Parana´-Paraguay basin. Migratory patterns are poorly  known and only few population genetic studies are available  for this species in the Upper Parana´. To assess genetic  population structure, we genotyped a sample of 93 individuals  from the Lower Uruguay River close to Gualeguaychu  ´ city (Entre R&#305;´os, Argentina) at three different  times, July 2008 (Winter), September 2008 (Spring) and  May 2009 (Fall). All individuals were genotyped for 12  microsatellite loci previously found to be informative to  assess populations of P. lineatus. Our results show seasonal  variation of the genetic sub-structuring at this locality that  may be related to the presence of different migratory stocks  throughout the year. The Fall sample includes an additional  genetic cluster of individuals not detected in Winter and  Spring, suggesting that this species should be considered a  mixed stock fishery.","palabras_clave":"microsatellites|Prochilodus|population structure|migratory fish"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64774,"idioma_id":4,"titulo":"Centrosomal AKAP350 modulates   the G  1\/S transition","resumen":"AKAP350 (AKAP450\/AKAP9\/CG-NaP) is an A-kinase anchoring protein, which recruits multiple signaling proteins to the Golgi apparatus and the centrosomes. Several proteins recruited to the centrosomes by this scaffold participate in the regulation of the cell cycle. Previous studies indicated that AKAP350 participates in centrosome duplication. In   the present study we specifically assessed the role of aKaP350 in the progression of the cell cycle. Our results showed that interference with aKaP350 expression inhibits G1\/s transition, decreasing the initiation of both DNa synthesis and centrosome duplication. We identified an AKAP350 carboxyl-terminal domain (aKaP350CTD), which contained the centrosomal targeting domain of aKaP350 and induced the initiation of DNa synthesis. Nevertheless, aKaP350cTD expression did not induce centrosomal duplication. aKaP350cTD partially delocalized endogenous aKaP350 from the   centrosomes, but increased the centrosomal levels of the cyclin-dependent kinase 2 (cdk2). Accordingly, the expression of this aKaP350 domain increased the endogenous phosphorylation of nucleophosmin by cdk2, which occurs at the G1\/s transition and is a marker of the centrosomal activity of the cyclin e-cdk2 complex. cdk2 recruitment to the centrosomes   is a necessary event for the development of the G1\/s transition. Altogether, our results indicate that aKaP350 facilitates the initiation of DNa synthesis by scaffolding cdk2 to the centrosomes, and enabling its specific activity at this organelle. Although this mechanism could also be involved in AKAP350-dependent modulation of centrosomal duplication, it is not sufficient to account for this process.","palabras_clave":"AKAP350|CELL CYCLE|CENTROSOMES|CDK2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64776,"idioma_id":4,"titulo":"Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome","resumen":"In this study, the characterization of insulin (auto)antibodies has been             described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes             by Surface Plasmon Resonance (SPR) in two particular clinical cases of             individuals with severe episodes of impaired glycemia. Subject 1 suffers from             brittle diabetes associated with circulating insulin antibodies (IA) due to insulin             treatment. Subject 2 has insulin autoantibodies (IAA) associated with             hypoglycemia in spite of not being diabetic and not having ever received             exogenous insulin therapy. After conventional screening for IA\/IAA by             radioligand binding assay (RBA), we further characterized IA\/IAA in sera of both             patients in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by             means of SPR technology. In both cases, q values were higher and Ka values             were lower than those obtained in type 1 diabetic patients, suggesting that             IA\/IAA:insulin immunocomplexes could be responsible for the uncontrolled             glycemia. Moreover, subject 1 had a predominat IgG1 response and subject 2             had an IgG3 response.             In conclusion, SPR technology is useful for the complete characterization of             IA\/IAA which can be used in special cases where the simple positive\/negative             determination is not enough to achieve a detailed description of the disease           fisiopathology.","palabras_clave":"INSULIN (AUTO)ANTIBODIES|HYPO AND HYPERGLYCEMIA|SURFACE PLASMON RESONANCE|CONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64777,"idioma_id":4,"titulo":"Progesterone protective effects in Neurodegeneration and Neuroinflammation","resumen":"Progesterone is a neuroprotective, promyelinating and anti-inflammatory factor for the nervoussystem. Here, we review the effects of progesterone in models of motoneurone degenerationand neuroinflammation. In neurodegeneration of the Wobbler mouse, a subset of spinal cordmotoneurones showed increased activity of nitric oxide synthase (NOS), increased intramitochondrialNOS, decreased activity of respiratory chain complexes, and decreased activity andprotein expression of Mn-superoxide dismutase type 2 (MnSOD2). Clinically, Wobblers sufferedseveral degrees of motor impairment. Progesterone treatment restored the expression of neuronalmarkers, decreased the activity of NOS and enhanced complex I respiratory activity andMnSOD2. Long-term treatment with progesterone increased muscle strength, biceps weight andsurvival. Collectively, these data suggest that progesterone prevented neurodegeneration. Tostudy the effects of progesterone in neuroinflammation, we employed mice with experimentalautoimmune encephalomyelitis (EAE). EAE mice spinal cord showed increased mRNA levels ofthe inflammatory mediators tumour necrosis factor (TNF)a and its receptor TNFR1, the microglialmarker CD11b, inducible NOS and the toll-like receptor 4. Progesterone pretreatment of EAEmice blocked the proinflammatory mediators, decreased Iba1+ microglial cells and attenuatedclinical signs of EAE. Therefore, reactive glial cells became targets of progesterone anti-inflammatoryeffects. These results represent a starting point for testing the usefulness of neuroactivesteroids in neurological disorders.","palabras_clave":"PROGESTERONA|NEUROPROTECCION|NEUROINFLAMACION|WOBBLER"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64778,"idioma_id":4,"titulo":"LATEST PLEISTOCENE AND HOLOCENE GLACIER FLUCTUATIONS IN SOUTHERNMOST TIERRA DEL FUEGO, ARGENTINA","resumen":"Some researchers propose that summer insolation controls long-term changes in glacier extent during the Holocene. If this hypothesis is correct, the record of glacier fluctuations at high latitudes in the Southern Hemisphere should differ from that in the Northern Hemisphere. Although the chronology of Holocene glacier fluctuations in the Northern Hemisphere is well established, much uncertainty remains in the ages of Holocene glacier fluctuations in the Southern Hemisphere, especially South America. Here we report on latest Pleistocene and Holocene glacier fluctuations at the southern end of the Andes north and west of Ushuaia, Argentina. Surface exposure ages (10Be) from glaciated bedrock beyond cirque moraines indicate that alpine areas were free of ice by ca. 16.9 ka. One, and in some cases two, closely spaced moraines extend up to 2 km beyond Little Ice Age moraines within many of the cirques in the region. The mean age of five 10Be ages from two pre-Little Ice Age moraines is 14.27 ? 12.67 ka, whereas a minimum limiting radiocarbon age for a smaller, recessional moraine in one cirque is 12.38-12.01 ka. Our ages imply that, following glacier retreat beginning about 18.52-17.17 ka, cirque glaciers first advanced during the Antarctic Cold Reversal (14.5-12.9 ka) and may have later advanced or stabilized in the Younger Dryas Chronozone (12.9-11.7 ka). Based on the distribution of thick, geochemically distinct, and well-dated Hudson tephra, no Holocene moraines appear to be older than 7.96-7.34 ka. At some sites, there is evidence for one or more advances of glaciers sometime between 7.96-7.34 ka and 5.29-5.05 ka to limits only tens of meters beyond Little Ice Age maximum positions. Taken together, the data: 1) do not support the summer insolation hypothesis to explain Holocene glacier fluctuations in southernmost Patagonia; 2) confirm paleobotanical evidence for a warm, dry early Holocene; and 3) suggest that some glaciers in the region reached extents comparable to those of the Little Ice Age shortly before 5.29-5.05 ka.","palabras_clave":"CIRQUE GLACIERS|NEOGLACIATIONS|HOLOCENE|FUEGIAN ANDES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64780,"idioma_id":5,"titulo":"Articulando comunidades de aprendizajes. La transición entre el aula y el territorio en la formación en Economía Social y Solidaria","resumen":".","palabras_clave":"Economía Social y Solidaria|Universidad|Educación Popular|Comunidad de Aprendizaje"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64781,"idioma_id":4,"titulo":"Design, Synthesis and Biological Evaluation of Sulfur-Containing 1,1-Bisphosphonic Acids as Antiparasitic Agents","resumen":"  As part of our efforts aimed at searching for new antiparasitic agents, 2-alkylmercaptoethyl-1,1- bisphosphonate derivatives were synthesized and evaluated against Trypanosoma cruzi, the etiologic agent of Chagas disease, and Toxoplasma gondii, the responsible agent for toxoplasmosis. Many of these sulfur-containing bisphosphonates were potent inhibitors against the intracellular form of T. cruzi, the clinically more relevant replicative form of this parasite, and tachyzoites of T. gondii targeting T. cruzi or T. gondii farnesyl diphosphate synthases (FPPSs), which constitute valid targets for the chemotherapy of these parasitic diseases. Interestingly, long chain length sulfur-containing bisphosphonates emerged as relevant antiparasitic agents. Taking compounds 37, 38, and 39 as representative members of this class of drugs, they exhibited ED50 values of 15.8 mM, 12.8 mM, and 22.4 mM, respectively, against amastigotes of T. cruzi. These cellular activities matched the inhibition of the enzymatic activity of the target enzyme (TcFPPS) having IC50 values of 6.4 mM, 1.7 mM, and 0.097 mM, respectively. In addition, these compounds were potent anti-Toxoplasma agents. They had ED50 values of 2.6 mM, 1.2 mM, and 1.8 mM, respectively, against T. gondii tachyzoites, while they exhibited a very potent inhibitory action against the target enzyme (TgFPPS) showing IC50 values of 0.024 mM, 0.025 mM, and 0.021 mM, respectively. Bisphosphonates bearing a sulfoxide unit at C-3 were also potent anti-Toxoplasma agents, particularly those bearing long aliphatic chains such as 43!45, which were also potent antiproliferative drugs against tachyzoites of T. gondii. These compounds inhibited the enzymatic activity of the target enzyme (TgFPPS) at the very low nanomolar range. These bisphosphonic acids have very good prospective not only as lead drugs but also as potential chemotherapeutic agents.Trypanosoma cruzi, the etiologic agent of Chagas disease, and Toxoplasma gondii, the responsible agent for toxoplasmosis. Many of these sulfur-containing bisphosphonates were potent inhibitors against the intracellular form of T. cruzi, the clinically more relevant replicative form of this parasite, and tachyzoites of T. gondii targeting T. cruzi or T. gondii farnesyl diphosphate synthases (FPPSs), which constitute valid targets for the chemotherapy of these parasitic diseases. Interestingly, long chain length sulfur-containing bisphosphonates emerged as relevant antiparasitic agents. Taking compounds 37, 38, and 39 as representative members of this class of drugs, they exhibited ED50 values of 15.8 mM, 12.8 mM, and 22.4 mM, respectively, against amastigotes of T. cruzi. These cellular activities matched the inhibition of the enzymatic activity of the target enzyme (TcFPPS) having IC50 values of 6.4 mM, 1.7 mM, and 0.097 mM, respectively. In addition, these compounds were potent anti-Toxoplasma agents. They had ED50 values of 2.6 mM, 1.2 mM, and 1.8 mM, respectively, against T. gondii tachyzoites, while they exhibited a very potent inhibitory action against the target enzyme (TgFPPS) showing IC50 values of 0.024 mM, 0.025 mM, and 0.021 mM, respectively. Bisphosphonates bearing a sulfoxide unit at C-3 were also potent anti-Toxoplasma agents, particularly those bearing long aliphatic chains such as 43!45, which were also potent antiproliferative drugs against tachyzoites of T. gondii. These compounds inhibited the enzymatic activity of the target enzyme (TgFPPS) at the very low nanomolar range. These bisphosphonic acids have very good prospective not only as lead drugs but also as potential chemotherapeutic agents.","palabras_clave":"Bisphosphonates|Farnesyl pyrophosphate synthase|Trypanosoma cruzi|Toxoplasma gondii"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64782,"idioma_id":5,"titulo":"Nanosuspensiones: Disminución del tamaño de partícula como herramienta para mejorar la biodisponibilidad de fármacos liposolubles","resumen":"En los últimos años, las nanosuspensiones han sido objeto de numerosos estudios con el fin de evaluar sus propiedades biofarmacéuticas, demostrando en muchos casos valiosas ventajas frente a las formulaciones utilizadas convencionalmente. El uso de esta novedosa tecnología ha permitido el desarrollo de formas farmacéuticas para ser administradas por diferentes vías y también su aplicación ha demostrado ventajas en el desarrollo de estudios toxicológicos.  En el presente trabajo se plantea una revisión de los aportes que brinda la obtención de partículas nanométricas de principios activos a la tecnología farmacéutica, los procesos de obtención de las mismas, así como su caracterización y principales aplicaciones en diferentes vías de administración de fármacos.","palabras_clave":"AUMENTO DE LA BIODISPONIBILIDAD|FÁRMACOS LIPOSOLUBLES|NANOSUSPENSIONES|VÍAS DE ADMINISTRACIÓN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64783,"idioma_id":4,"titulo":"Mitochondrial dysfunction in brain cortex mitochondria of STZ-diabetic rats. Effect of L-arginine","resumen":"Abstract            Mitochondrial dysfunction has been implicated in many diseases, including diabetes. It is well known that oxygen free radical species are produced endogenously by mitochondria, and also nitric oxide (NO) by nitric oxide synthases (NOS) associated to mitochondrial membranes, in consequence these organelles constitute main targets for oxidative damage. The aim of this study was to analyze mitochondrial physiology and NO production in brain cortex mitochondria of streptozotocin (STZ) diabetic rats in an early stage of diabetes and the potential effect of L-arginine administration. The diabetic condition was characterized by a clear hyperglycaemic state with loose of body weight after 4&nbsp;days of STZ injection. This hyperglycaemic state was associated with mitochondrial dysfunction that was evident by an impairment of the respiratory activity, increased production of superoxide anion and a clear mitochondrial depolarization. In addition, the alteration in mitochondrial physiology was associated with a significant decrease in both NO production and nitric oxide synthase type I (NOS&nbsp;I) expression associated to the mitochondrial membranes. An increased level of thiobarbituric acid-reactive substances (TBARS) in brain cortex homogenates from STZ-diabetic rats indicated the presence of lipid peroxidation. L-arginine treatment to diabetic rats did not change blood glucose levels but significantly ameliorated the oxidative stress evidenced by lower TBARS and a lower level of superoxide anion. This effect was paralleled by improvement of mitochondrial respiratory function and a partial mitochondrial repolarization.In addition, the administration of L-arginine to diabetic rats prevented the decrease in NO production and NOSI expression. These results could indicate that exogenously administered L-arginine may have beneficial effects on mitochondrial function, oxidative stress and NO production in brain cortex mitochondria of STZ-diabetic rats.","palabras_clave":"Brain cortex mitochondria|Diabetes|Nitric oxide|L-Arginine"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64784,"idioma_id":4,"titulo":"Bionanoparticles, a green nanochemistry approach","resumen":"During the last decades the synthesis of  nanoparticles has been reported expansively because new applications on  research and industry were found. Different types of mechanistic synthesis of nanoparticles  from metallic, polymeric, and hybrid materials are currently on development.  The use of diverse biological materials such as plants, and many microbes to  synthesize nanoparticles has been described. However, two main avenues for  nanoparticles synthesis: cell-free extract and cell cultivation have been  reported. The state of art of both biotechnological approaches for different  type nanoparticles are reviewed in the present work(*).(*) Special issue on Process Biochemistry dedicated to Dr. Rodolfo Ertola","palabras_clave":"nanoparticulas|fermentacion|microorganismos"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64785,"idioma_id":4,"titulo":"Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment","resumen":"A new formulation is described based on biopolymeric microspheres containing alginate lyase(AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) andhigh-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix.ALG microspheres coated with HMP and ALG-HMP blend are gelled in water\/organic solventsmixtures, obtaining Cip encapsulations from 46.0 to100.0%. ALG-HMP shows a Cip sustainable releaseprofile and is able to encapsulate 90.0% of AL, showing76.0% enzyme activity after release under simulatedintestinal conditions. The developed system is apromising delivery carrier to treat chronic infectionof Pseudomonas aeruginosa and to reduce theviscoelasticity of the mucus accumulated into intestineof CF patients.","palabras_clave":"biopolimeros|liberacion controlada|fibrosis quisitica|alginato liasa"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64786,"idioma_id":4,"titulo":"Molecular characterization of Cryptosporidium isolates from calves in Argentina","resumen":"Cryptosporidiosis is responsible for significant fatalities of neonatal calves, resulting in substantial economic loss in dairy farming in several countries. Additionally, the high shedding of environmentally resistant oocysts by calves promotes contamination of drinking water and facilitates outbreaks of cryptosporidiosis in humans. Here we report on the Cryptosporidium species and GP60 subtypes of 45 calves originating from the Humid Pampa, the main productive dairy farming area of Argentina. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene was done to determine the infecting Cryptosporidium species and only Cryptosporidium parvum was detected. Subtyping by sequence analysis of the GP60 gene revealed 6 different alleles all pertaining to the zoonotic IIa family. Of these, IIaA23G1R1 represents a novel IIa subtype. Other identified subtypes, IIa18G1R1, IIaA20G1R1, IIaA21G1R1, and IIaA22G1R1 have been recognized in very few studies and\/or with low frequencies. Interestingly, different alleles prevailed in the provinces of Buenos Aires (IIaA17G1R1 and IIaA21G1R1), Santa Fe (IIaA23G1R1), and Cordoba (IIaA20G1R1 and IIaA21G1R1), and different allele distribution patterns were observed. Subtypes IIaA18G1R1 and IIaA17G1R1, the latter often found in this study, are strongly implicated in zoonotic transmission, suggesting that calves may represent a potential source for human cryptosporidiosis in this region. This is the first published report of a molecular analysis of Cryptosporidium infection in dairy and beef calves from Argentina.","palabras_clave":"CRYPTOSPORIDIOSIS|CALVES|18S rRNA|GP60"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64787,"idioma_id":4,"titulo":"'Temperature Induced Syllable Breaking Unveils Nonlinearly Interacting Timescales in Birdsong Motor Pathway.'","resumen":"The nature of telencephalic control over premotor and motor circuits is   debated. Hypotheses range from complete usurping of downstream circuitry   to highly interactive mechanisms of control. We show theoretically and   experimentally, that telencephalic song motor control in canaries is   consistent with a highly interactive strategy. As predicted from a   theoretical model of respiratory control, mild cooling of a forebrain   nucleus (HVC) led to song stretching, but further cooling caused   progressive restructuring of song, consistent with the hypothesis that   respiratory gestures are subharmonic responses to a timescale present in   the output of HVC. This interaction between a life-sustaining motor   function (respiration) and telencephalic song motor control suggests a   more general mechanism of how nonlinear integration of evolutionarily   new brain structures into existing circuitry gives rise to diverse, new   behavior.","palabras_clave":"birdsong  |temperature|respiration|motor control|subharmonics|timescales|locking"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64788,"idioma_id":4,"titulo":"Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations","resumen":"  A simple and efficient liquid chromatographic method has been developed and validated for  the simultaneous determination of bromhexine, chlorpheniramine, paracetamol, and pseudoephedrine  in common cold medications (tablets and syrups). The separation of the analytes was  achieved within 10 min, employing a mixture of 10mM triethylamine-phosphoric acid buffer  (pH 4.0) and MeOH (35:65, v=v) as isocratic mobile phase, pumped at 1.0 mL min1 through  a cyano column (5 lm particle size). The analytes were detected at 215 nm. Statistical experimental  designs and graphic representations (response surface methodologies, Pareto charts) were used for  selecting the proper detection wavelength, optimizing the mobile phase composition, and assessing  method robustness.  The linearity of the calibration (r&gt;0.99, n¼21) in the relevant ranges (up to 130% of the  expected concentrations of the analytes in the formulations), method accuracy (bias&lt;2.0%),  repeatability (RSD&lt;2.0%) and intermediate precision, were verified. In addition, specificity (peak  purities with photodiode array detector &gt;0.9997) and method robustness were evaluated, and  system suitability parameters were determined.  The validated method was successfully employed for the routine analysis of various commercial  tablet and syrup pharmaceutical preparations against the common cold, showing satisfactory  analyte recoveries and RSD values.","palabras_clave":"bromhexine|chlorpheniramine|experimental design|paracetamol"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64789,"idioma_id":4,"titulo":"Lysosomal and mitochondrial permeabilization mediates Zinc (II) cationic phthalocyanine phototoxicity","resumen":"In order to find a novel photosensitizer to be used in photodynamic therapy for cancer treatment, wehave previously showed that the cationic zinc(II) phthalocyanine named Pc13, the sulfur-linked dye2,9(10),16(17),23(24)-tetrakis[(2-trimethylammonium) ethylsulfanyl]phthalocyaninatozinc(II) tetraio-dide, exerts a selective phototoxic effect on human nasopharynx KB carcinoma cells and induces anapoptotic response characterized by an increase in the activity of caspase-3. Since the activation of anapoptotic pathway by chemotherapeutic agents contributes to the elimination of malignant cells, in thisstudy we investigated the molecular mechanisms underlying the antitumor action of Pc13. We found thatafter light exposure, Pc13 induced the production of reactive oxygen species (ROS), which are mediatingthe resultant cytotoxic action on KB cells. ROS led to an early permeabilization of lysosomal membranes asdemonstrated by the reduction of lysosome fluorescence with acridine orange and the release of lysoso-mal proteases to cytosol. Treatment with antioxidants inhibited ROS generation, preserved the integrityof lysosomal membrane and increased cell proliferation in a concentration-dependent manner. Lysosomedisruption was followed by mitochondrial depolarization, cytosolic release of cytochrome C and caspasesactivation. Although no change in the total amount of Bax was observed, the translocation of Bax fromcytosol to mitochondria, the cleavage of the pro-apoptotic protein Bid, together with the decrease ofthe anti-apoptotic proteins Bcl-XLand Bcl-2 indicated the involvement of Bcl-2 family proteins in theinduction of the mitochondrial pathway. It was also demonstrated that cathepsin D, but not caspase-8,contributed to Bid cleavage. In conclusion, Pc13-induced cell photodamage is triggered by ROS genera-tion and activation of the mitochondrial apoptotic pathway through the release of lysosomal proteases.In addition, our results also indicated that Pc13 induced a caspase-dependent apoptotic response, beingactivation of caspase-8, -9 and -3 the result of a post-mitochondrial event.","palabras_clave":"ZINC(II)CATIONIC PHTHALOCYANINE |PHOTOTOXICITY|LYSOSOMAL PERMEABLIZATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64790,"idioma_id":4,"titulo":"Jet energy resolution in proton-proton collisions at s&#8730;=7 TeV recorded in 2010 with the ATLAS detector","resumen":"The measurement of the jet energy resolution is presented using data recorded with the ATLAS detector in proton-proton collisions at s&#8730;=7 TeV . The sample corresponds to an integrated luminosity of 35 pb&#8722;1. Jets are reconstructed from energy deposits measured by the calorimeters and calibrated using different jet calibration schemes. The jet energy resolution is measured with two different in situ methods which are found to be in agreement within uncertainties. The total uncertainties on these measurements range from 20 % to 10 % for jets within |y|<2.8 and with transverse momenta increasing from 30 GeV to 500 GeV. Overall, the Monte Carlo simulation of the jet energy resolution agrees with the data within 10 %.","palabras_clave":"ATLAS|JET|RESOLUTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64791,"idioma_id":4,"titulo":"'Photoreduction of aliphatic and aromatic thioketals. New access to the reduction of carbonyl groups by a desulfurization chain process'","resumen":"Aromatic and aliphatic ketones can be converted to methylene groups by desulfurization of the corresponding dithioketals in moderate to good yields. The reaction proceeds by photoinduced electron transfer from  tert-BuOK in the presence of 1,4-dicyclohexadiene as hydrogen atom donor. The diene is able to trigger and keep a chain process by hydrogen transfer-proton transfer reactions.","palabras_clave":"Photoreduction|Dithianes|Carbonyl Group|Radical Anion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64792,"idioma_id":4,"titulo":"Expression of the FUT2 gene and CD44 marker in patients with oral lesions","resumen":"The aim of this work was to investigate the FUT 2 gene, the secretor status and the expression of CD44 protein in epithelial cells obtain from saliva samples from patients with oral lesions (benign, pre-cancerous and cancerous lesions, n = 94). We analyzed polymorphisms ofthe FUT2 gene by allele specific oligonucleotide?polymerase chain reaction. The FUT2 gene encodes the (1,2) fucosyltransferase (Se enzyme) that regulates the expression of ABH antigens in secretions. Generally speaking, being a non-secretor has several disadvantages with regard to metabolism and immune function. In this study, we found that oral pre-cancerous and cancerous lesions were increased among individuals with non-secretor status and nonsense mutation 428G&#8594;A. Fifty-one percent of the patients with oral pre-malignant and malignant lesions were non-secretors, in contrast with the healthy population (OR = 3.44). We observed a marginal association between secretor status and these lesions. Our study suggests that the lack of wild type FUT2 gene and a non-secretor status appear to be an associated risk marker for the development of oral cancer in patients with oral lesions. One of the genes involved in tumour processes is CD44, which appears to be one of the most promising candidates as a cancer diagnosis marker. We investigated, using confocal microscopy, the expression of CD44 protein in epithelial cells obtained from saliva samples from patients with oral lesions. The results obtained showed fluorescence corresponding to the presence of CD44 protein in samples from patients diagnosed with cancer and precancer. These findings indicate that overexpression of CD44 molecule analyzed could be considered as a marker of risk in individuals with oral lesions. © 2013 Sociedad Española de Inmunología. Published by Elsevie","palabras_clave":"GEN FUT 2|CD44|LESIONS CANCEROSAS|BLOOD GROUP ANTIGENS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64793,"idioma_id":4,"titulo":"In situ polymerization of styrene in AAO nanocavities","resumen":"One of the most promising aspects of the alumina oxide (AAO) template is the ability to generate a variety of different hierarchical one-dimensional (1D) polymer morphologies with structural definition on the nanometric scale. In-situ polymerization of monomers in reduced spaces of porous aluminum oxide (AAO) nanocavities can give rise to the direct production of versatile polymer nanostructures. In this work, porous anodic aluminum oxide (AAO) devices of 35 nm of diameter have been obtained by a two-step electrochemical anodization process and used as a nanoreactor to study the radical polymerization kinetics of styrene (St) in confinement and the results compared to those of polymerization in bulk.  SEM morphological study has been conducted to establish the final structure of obtained polymer nanostructures.  Confocal Raman microscopy has been performed to study the formation of the polymer through the AAO cavities as a function of time and with this methodology it has been possible to establish the monomer conversion for styrenic polymerization in AAO devices. Polystyrene obtained in the nanoreactor was characterized by SEC, NMR, TGA and DSC and the properties compared with those of bulk polymer. It was found that both the average molecular weights and polydispersity index of nanostructured polymer are lower than those obtained for bulk polymer.  NMR studies have shown that the use of a reactor with nanometric size dimensions gave the obtained polystyrene greater stereospecificity than that obtained in bulk. Thermal stability and Tg values are higher for nanostructured than bulk polymers. Moreover, the methodology proposed in this work, using AAO nanocavities as nanoreactors for polymerization reaction, can be generalized and applied to obtain polymer nanostructures of very different chemical nature and morphology by choosing the appropriate monomer or monomer reactants and by tailoring the dimension of AAO cylindrical nanocavities, that is, diameter from 20 to 400 nm and length from a few to hundreds of microns.","palabras_clave":"Nanofabrication|Nanoconfinement|Microstructure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64794,"idioma_id":4,"titulo":"Self-assembly of poly(benzyl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate) diblock copolymer films at the air\/water interface and deposition on solid substrates via Langmuir-Blodgett transfer: aggregation behavior, morphological characteri","resumen":"The aggregation behavior and morphological characteristics of amphiphilic block copolymer polybenzyl methacrylate-block-poly(dimethylamino)ethyl methacrylate (PBzMA-b-PDMAEMA) at the air?water interface were investigated through surface pressure measurements, atomic force microscopy (AFM)imaging, electrochemical measurements and X-ray reflectivity. Ionization of PDMAEMA blocks significantly affects the isotherms of the surface films at the air?water interface and, consequently, originates different morphologies of Langmuir?Blodgett films obtained under different experimental conditions. At low pH the PDMAEMA blocks are fully protonated and therefore dissolved in the  aqueous subphase. Under this condition, the effects of the solubility and electrostatic repulsion of  submerged PDMAEMA chains prevail over hydrophobic interactions and the Langmuir film exhibits low surface pressure at large molecular area values. Upon compression, the isotherm shows a pseudoplateau  corresponding to the ?pancake-to-brush? transition followed by an increase in surface pressure at smaller MMA values. These results were correlated with the morphological features of Langmuir?Blodgett films transferred onto silicon substrates, where dispersed dot-like domains that gradually transformed into an island-like structure, followed by further percolation into a continent-like morphology, were observed through AFM imaging. On the other hand, at high pH the isotherm is more  expanded and the film exhibits higher surface pressure at relatively high MMA values due to the strong repulsive interactions between interface-confined hydrophobic aggregates constituted of PBzMA cores and neutral PDMAEMA shells. In this case, the AFM results show a structural evolution from circular and quasi-hexagonally packed micelles, followed by a worm-like structure that collapses into a homogeneous film. Furthermore, the study of the copolymer behavior under different subphase ionic strength conditions confirmed the critical role of electrostatic interactions in determining the characteristics of the isotherm. We could also demonstrate that our system follows accurately the scaling relationship for surface pressure of annealed brushes. Complementary studies performed by means of X-ray reflectivity were carried out to probe the buried interfacial structure of the diblock copolymer film, corroborating that the organization of both blocks on the silicon substrates is strongly  dependent on the pH conditions of the subphase during the LB transfer.","palabras_clave":"BLOCK COPOLYMER|FILM|LANGMUIR-BLODGETT|AGGREGATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64795,"idioma_id":4,"titulo":"Hyaluronan Degrading Silica Nanoparticles for Skin Cancer Therapy","resumen":"Wereport the first nanoformulation of Hyaluronidase (Hyal) and its enhancedadjuvant effect over the free enzyme. Hyaluronic acid (HA)- degrading enzyme Hyalwas immobilized on 250nm Silica Nanoparticles (SiNp) maintaining specificactivity of the enzyme, via the layer-by-layer self-assembly technique. This processwas characterized by Dynamic light scattering (DLS), Zeta Potential, Infrared andUV-Vis spectroscopy, Transmission electron microscopy (TEM) and enzymatic activitymeasurements. The nanoparticles were tested invivo as adjuvants of Carboplatin (CP), peritumorally injected in A375 humanmelanoma bearing mice and compared with the non-inmobilized enzyme, on thebasis of equal enzymatic activity. Alcian Blue staining of A375 tumorsindicated large overexpression of Hyaluronan. At the end of the experiment, tumorvolume reduction with SiNp-immobilized Hyal was significantly enhanced comparedto non-immobilized Hyal. Field emission scanning electron microscopy images (FE-SEM)together with energy dispersive X-Ray (EDS) confirmed the presence of SiNp onthe tumor. We mean a proof of concept: this extracellular matrix (ECM) degradingenzyme, immobilized on SiNp, is a more effective local adjuvant of cancer drugsthan the non-immobilized enzyme. This could prove useful in future therapiesusing other or a combination of ECM degrading enzymes.","palabras_clave":"Silica Nanoparticles|Layer by Layer Self assembly|Hyaluronidase|Adjuvant|Anticancer drug|Melanoma"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64796,"idioma_id":4,"titulo":"Lack of Functional GABA B Receptors Alters Kiss1 , Gnrh1 and Gad1 mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4","resumen":"                    Background\/Aims: Adult  mice lacking functional GABAB receptors (GABAB1KO) show  altered Gnrh1 and Gad1 expressions in the preoptic  area-anterior hypothalamus (POA-AH) and females display disruption of cyclicity  and fertility. Here we addressed whether sexual differentiation of the brain  and the proper wiring of the GnRH and kisspeptin systems were already disturbed  in postnatal day 4 (PND4) GABAB1KO mice.                    Methods: PND4  wild type (WT) and GABAB1KO mice of both sexes were sacrificed;  tissues were collected to determine mRNA expression (qPCR), amino acids (HPLC),  and hormones (RIA and\/or IHC).                     Results: &nbsp;GnRH neuron number (IHC) did not differ among groups  in olfactory bulbs or OVLT-POA. Gnrh1  mRNA (qPCR) in POA-AH was similar among groups. Gnrh1 mRNA in medial basal hypothalamus (MBH) was similar in WTs  but was increased in GABAB1KO females compared to GABAB1KO  males. Hypothalamic GnRH (RIA) was sexually different in WTs (males &gt; females) but this sex difference was  lost in GABAB1KOs; the same pattern was observed when analyzing only  the MBH, but not in the POA-AH. Arcuate nucleus Kiss1  mRNA (micropunch-qPCR) was higher in WT females than in WT males and GABAB1KO  females. Gad1  mRNA in MBH was increased in GABAB1KO females compared to GABAB1KO  males. Serum  LH and gonadal estradiol content were also increased in GABAB1KOs.   Conclusion:  We demonstrate  that GABABRs participate in the sexual differentiation of the ARC\/MBH, because sex  differences in several reproductive genes, such as Gad1, Kiss1 and Gnrh1, are critically disturbed in GABAB1KO  mice at PND4, probably altering the organization and development of neural  circuits governing the reproductive axis.","palabras_clave":"GABA B receptors|GnRH|GABA|Kiss1|Gonadotropins|Sex Steroids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64798,"idioma_id":4,"titulo":"Electronic Paramagnetic Resonance (EPR) for the Study of Ascorbyl Radical and Lipid Radicals in Marine Organisms","resumen":"&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Electron paramagnetic resonance (EPR) spectroscopy detects the presence of radicals of biological interest, such as ascorbyl radical (A?) and lipid radicals. A? is easily detectable by EPR even in aqueous solution at room-temperature. Under oxidative conditions leading to changes in total ascorbate (AH¡¥) content, the A?\/AH¡¥ ratio could be used to estimate early oxidative stress in the hydrophilic milieu. This methodology was applied to a wide range of aquatic systems including algae, sea urchin, limpets, bivalves and fish, under physiological and oxidative stress conditions as well. The A?\/AH¡¥ ratio reflected the state of one part of the oxidative defense system and provided an early and simple diagnosis of environmental stressing conditions. Oxidative damage to lipids was assessed by the EPR-sensitive adduct formation that correlates well with cell membrane damage with no interference from other biological compounds. Probe instability, tissue metabolism, lack of spin specificity are drawback factors for employing EPR for in vivo determination of free radicals. However, the dependability of this technique, mostly by combining it with other biochemical strategies, enhance the value of these procedures as contributors to the knowledge of oxidative condition in aquatic organisms.                       ","palabras_clave":"EPR|invertebrates|ascorbyl radical|lipid radicals"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64799,"idioma_id":4,"titulo":"Nitric oxide and membrane lipid peroxidation in photosynthetic and non-photosynthetic organisms under several stress conditions","resumen":"The results summarized here implied the existence of a very complex regulatory interplay between NO and ROS. The multiple effects of NO on the process of lipid peroxidation imply that the net result will depend on the balance of competing factors. The rate and location of NO formation,and also the rate of formation of superoxide anion, or other mitigating factors,will all contribute to the degree and the nature of the effect on lipidoxidation in aparticular system. Detailed analys is of the molecular mechanisms in each condition is required.","palabras_clave":"NITRIC OXIDE|LIPID RADICALS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64800,"idioma_id":4,"titulo":"A hadronic scenario for  HESS J1818-154","resumen":"Aims. G15.4+0.1 is a faint supernova remnant (SNR) which has been recently associated with the&nbsp;  gamma-ray source HESS J1818-154.We investigate a hadronic scenario for the production of the gamma  -ray emission.Methods. Molecular 13CO (J=1-0) taken from the Galactic Ring Survey (GRS), and neutral hydrogen (HI) data from the SouthernGalactic Plane Survey (SGPS) have been used in combination with new 1420 MHz radio continuum observations carried out with theGiant Metrewave Radio Telescope (GMRT).Results. From the new observations and analysis of archival data we provided for the first time a reliable estimate for the distance tothe SNR G15.4+0.1 and discovered molecular clouds located at the same distance. On the basis of HI absorption features we estimatethe distance to G15.4+0.1 in 4.8+\/-1.0 kpc. The 13CO observations clearly show a molecular cloud about 5 arcmin in size with two brightclumps, labeled A and B, clump A positionally associated with the location of HESS J1818-154 and clump B in coincidence withthe brightest northern border of the radio SNR shell. The HI absorption and the 13CO emission study indicates a possible interactionbetween the molecular material and the remnant. We estimate the masses and densities of the molecular gas in (1.2+\/-0.5)×10^3 solar masses and (1.5 +\/- 0.4)×10^3 cm^&#8722;3 for clump A and (3.0+\/-0.7)×10^3 solar masses and (1.1+\/-0.3)×10^3 cm^-3 for clump B. Calculations show that the average density of the molecular clump A is sufficient to produce the detected gamma-ray flux, thus favouring a hadronic origin for the high-energy emission.","palabras_clave":"SNRS|ISM|HESS J1818-154|G15.4+0.1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64801,"idioma_id":4,"titulo":"Fluorescence of berberine in microheterogeneous systems","resumen":"Spectral properties of the alkaloid berberine were studied in micellar solution and microemulsions based on anionic sodium dodecyl sulfate, cationic cetyltrimethylammonium bromide and nonionic Triton X-100 surfactants. Absorption and fluorescence emission spectra were determined. For screening the influence of type and concentration of micelles on the fluorescence of berberine a 3 2 full factorial design was used. Higher responses were obtained when berberine was dissolved in sodium dodecyl sulfate micelles 0.01 M. Comparative results of fluorescence quantum yields (&#934; f ) reveal that the highest values (&#934; f &#8805;0.01) were observed in microemulsions. In the microheterogeneous systems investigated the most probable location of berberine is the micellar interfacial region.&nbsp;","palabras_clave":"Berberine|Fluorescence|Full factorial design|Micelles"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64802,"idioma_id":4,"titulo":"EFFECT OF MINERAL MATTER REMOVAL ON PYROLYSIS OF WOOD SAWDUST FROM AN INVASIVE SPECIES","resumen":"Kinetics of the pyrolysis of wood sawdust from the invasive species Parkinsonia aculeata, untreated and demineralized by a mild acid treatment, is comparatively investigated in order to examine the effect of the removal of minerals naturally present in the biomass. Non-isothermal thermogravimetric analysis from room temperature up to 500 °C is applied for this purpose. Demineralization shifts the process onset and the maximum degradation rate to higher temperatures, and leads to enhance the activation energy from 56 kJ\/mol to 60 kJ\/mol, pointing to a catalytic role of alkaline and alkaline earth metals in the biomass. Likewise, the three kinds of pyrolysis products (gas, bio-char and bio-oil) are obtained from experiments performed in a bench-scale installation at 500 °C. Yields and physicochemical characteristics of the pyrolysis products are determined. The pronounced reduction in the content of metals in the sawdust leads to increase bio-oil yield in around 10%, the specific surface area of the bio-char, from 2 m2\/g to 74 m2\/g, and the higher heating value of all the pyrolysis products","palabras_clave":"INVASIVE WOOD BIOMASS |PYROLYSIS|BIOENERGY|BIOFUELS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64803,"idioma_id":4,"titulo":"Incorporating phylogenetic uncertainty on phylogeny-based palaeontological dating and the timing of turtle diversification","resumen":"Methods improving the performance of molecular dating of divergence time of clades have improved dramatically in recent years.  The calibration of molecular dating using the first appearance of a clade in the fossil record is a crucial step towards inferring the  minimal diversification time of various groups and the choice of extinct taxa can strongly influence the molecular dates. Here, we  evaluate the uncertainty on the phylogenetic position of extinct taxa through non-parametric bootstrapping. The recognition of  phylogenetic uncertainty resulted in the definition of the Bootstrap Uncertainty Range (BUR) for the age of first appearance of a  given clade. The BUR is calculated as the interval of geological time in which the diversification of a given clade can be inferred to  have occurred, based on the temporal information of the fossil record and the topologies of the bootstrap trees. Divergence times  based on BUR analyses were calculated for three clades of turtles: Testudines, Pleurodira and Cryptodira. This resulted in extensive  uncertainty ranges of topology-dependent minimal divergence dates for these clades.","palabras_clave":"Tree calibration|phylogeny|phylogenetic uncertainty|paleontological dating"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64804,"idioma_id":4,"titulo":"New evidence from the Palaeocene of Patagonia (Argentina) on the evolution and palaeo-biogeography of Meiolaniformes (Testudinata, new taxon name)","resumen":"Peligrochelys walshae gen. et sp. nov. is a new stem turtle closely related to the clade  Meiolaniidae found in the Palaeocene levels of the Salamanca Formation  (Maastrichtian-Danian) of central Patagonia, Chubut Province, Argentina. A  phylogenetic analysis shows that Peligrochelys walshae gen. et sp. nov. forms a  monophyletic group with Mongolochelys efremovi, a clade in return that is the sister  group of a clade formed by Patagoniaemys gasparinae, Otwayemys cunicularius,  Kallokibotion bajazidi and Meiolaniidae. Meiolaniidae is recovered deeply nested in a  monophyletic group (Meiolaniformes, new taxon name) dominated by Gondwanan taxa  (e.g., Chubutemys copelloi, Pa. gasparinae, O. cunicularius, and Pe. walshae gen. et sp.  nov.), that also includes some notables Laurasian representatives (Mo. efremovi and K.  bajazidi). The biogeographic scenario presented here supports the hypothesis that this  group of turtles may have originated as early as the Early Cretaceous in the South  American part of Gondwana. The long ghost lineage that extends below this clade  suggests that it may have originated any time between the Early Jurassic and the Early  Cretaceous, implying a Pangaean or Gondwanan origin for the clade, respectively. The  phylogenetic scenario presented here shows that at least two lineages (Pe. walshae and  Meiolaniidae) of Meiolaniformes (new taxon name) survived the K-P boundary  extinction. Another interesting characteristic of meiolaniform turtles in the fossil record  is their frequent association with another clade of strictly Gondwanan distribution, the  pleurodiran clade Chelidae. This association is typically found in outcrops from the  Early Cretaceous to the Eocene in central Patagonia and in the Early Cretaceous and  from the Miocene to the Pleistocene in Australia.","palabras_clave":"Peligrochelys walshae|phylogeny|K-P extinction|Chelidae and Meiolaniidae association"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64805,"idioma_id":4,"titulo":"The sterol-C7 desaturase from the ciliate Tetrahymena thermophila is a Rieske oxygenase which is highl y conserved in animals","resumen":"The ciliate Tetrahymena thermophila incorporates sterols from its environment that desaturates at positions C5(6), C7(8) and C22(23). Phytosterols are additionally modified by removal of the ethyl group at C24. The enzymes involved are oxygen-, NAD(P)H- and cytochrome b5-dependent, reason why they were classified as members of the hydroxylases\/desaturases superfamily. The ciliate´s genome revealed the presence of seven putative sterol desaturases belonging to this family, two of which we have previously characterized as the C24-de-ethylase and C5(6)-desaturase. A Rieske oxygenase was also identified; this type of enzyme, with sterol C7(8)-desaturase activity, was observed only in animals, called Neverland in insects and DAF-36 in nematodes. They perform the conversion of cholesterol into 7-dehydrocholesterol, first step in the synthesis of the essential hormones ecdysteroids and dafachronic acids. By adapting an RNA interference-by-feeding protocol, we easily screened six of the eight genes described above, allowing the characterization of the Rieske-like oxygenase as the ciliate´s C7(8)-desaturase (Des7p). This characterization was confirmed by obtaining the corresponding knockout mutant, making Des7p the first non-animal Rieske-sterol desaturase described. To our knowledge, this is the first time that the feeding-RNAi technique was successfully applied in T. thermophila, enabling to consider such methodology for future reverse genetics-high throughput screenings in this ciliate. Bioinformatics analyses revealed the presence of Des7p orthologs in other Oligohymenophorean ciliates as well as in non-animal Opisthokonts, like the protists Salpingoeca rosetta and Capsaspora owczarzaki. A horizontal gene transfer event from a unicellular Opisthokont to an ancient phagotrophic Oligohymenophorean could explain the acquisition of the Rieske oxygenase by Tetrahymena.","palabras_clave":"RIESKE OXYGENASE|CILIATES|STEROL DESATURASE|PHYLOGENY|RNA INTERFERENCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64806,"idioma_id":4,"titulo":"Blue-light dependent inhibition of twitching motility in Acinetobacter baylyi ADP1: Additive involvement of three BLUF domain-containing proteins.","resumen":"Twitching motility in Acinetobacter baylyi ADP1 is inhibited by moderate intensities of blue light in a temperature-dependent manner (maximally at 20 °C). We analysed                     the involvement of four predicted blue-light sensing using flavin (BLUF)-domain-containing proteins encoded in the genome                     of this strain in the twitching motility phenotype. All four genes were expressed both in light and in darkness. A phylogenetic                     tree showed that one BLUF domain, ACIAD2110, grouped separately from the other three (ACIAD1499, ACIAD2125 and ACIAD2129).                     Individual knockout mutants of the latter three, but not of ACIAD2110, fully abolished the light dependency of the twitching                     motility response. Quantitative analysis of transcript level of the three genes showed a decreased expression in the light,                     with dark\/light ratios of 1.65±0.28, 1.79±0.21 and 2.69±0.39, for ACIAD2125, ACIAD2129 and ACIAD1499, respectively. Double                     and triple knockouts of ACIAD1499, ACIAD2125 and ACIAD2129 confirmed the same phenotype as the corresponding single knockouts.                     Complementation of all the single knockouts and the triple knockout mutants with any of the three BLUF-domain-encoding genes                     fully restored the inhibition of twitching motility by blue light that is observed in the wild-type strain. A. baylyi ADP1 therefore shows a high degree of redundancy in the genes that encode BLUF-containing photoreceptors. Moreover, all plasmid-complemented                     strains, expressing any of the BLUF proteins irrespective of the specific set of deleted photoreceptors, displayed increased                     light-dependent inhibition of twitching motility, as compared to the wild-type (P&lt;0.001). We conclude that the three genes ACIAD1499, ACIAD2125 and ACIAD2129 are jointly required to inhibit twitching motility                     under moderate blue-light illumination.","palabras_clave":"ACINETOBACTER|BLUE-LIGHT RECEPTORS|BLUF|TWITCHING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64807,"idioma_id":4,"titulo":"Morphological and functional effects on cardiac tissue induced by moderate zinc deficiency during prenatal and postnatal life in male and female rats","resumen":"&nbsp;The aim of this study was to evaluate whether moderate zinc  restriction in rats throughout fetal life, lactation and\/or post-weaning growth results in early changes in cardiac morphology predisposing  the onset of cardiac dysfunction in adult life, and sex-related differences in the  adaptation to this nutritional injury. Female Wistar rats received low or  control zinc diets from the beginning of pregnancy up to weaning. After  weaning, offspring were fed either a low or control zinc diet until 81 days. Systolic  blood pressure was measured. Echocardiographic and electrocardiographic examinations,  morphological studies and apoptosis by TUNEL assay were performed in left  ventricle. In early stages, zinc-deficient male and female offspring showed an  increase in cardiomyocyte diameter, probably associated with an increase in cardiac  apoptotic cells, but smaller myocyte diameters in adulthood. In adult males, this nutritional injury induced  decreased contractility and dilatation of the left ventricle, not allowing the  heart to compensate the higher levels of blood pressure, and hypertrophic  remodeling of coronary arteries associated with increased blood pressure. Adequate zinc intake during post-weaning life did not  overcome blood pressure levels but reversed some of the detrimental effects of  earlier zinc deficiency in cardiac morphology and function. Females were  less sensitive to this deficiency, exhibiting normal levels of blood pressure and  no structural or functional heart alterations in adult life. The present study demonstrate that the effects of  zinc deficiency on blood pressure, cardiac morphology and function differ  between sexes, with males more predisposed to develop cardiovascular diseases  in adulthood.            ","palabras_clave":"PRENATAL AND POSTNATAL ZINC DEFICIENCY|SEX DIFFERENCES|HEART|MORPHOLOGY AND FUNCTION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64808,"idioma_id":4,"titulo":"Hydrogen desorption from a hydride container under different heat exchange conditions","resumen":"bstract    The desorption behavior of a hydrogen storage prototype loaded with AB5H6   hydride, whose equilibrium pressure makes it suitable for both feeding a   PEM fuel cell and being charged directly from a low pressure water   electrolyzer without need of additional compression, was studied. The   nominal 70 L hydrogen storage capacity of the container (T = 20 C, P =   101.3 kPa) suffices for ca. 2.5 h operation of a 50 W hydrogen\/oxygen   fuel cell stack. The hydride container is provided with aluminum   extended surfaces to enhance heat exchange with the surrounding medium.   These surfaces consist of internal disk-shaped metal foils and external   axial fins. The characterization of the storage prototype at different   hydrogen discharge flow rates was made by monitoring the internal   pressure and the temperatures of the external wall and at the center   inside the container. The response of the storage device was tested at   room temperature under different conditions such as natural convection   in air and forced air ventilation, and at different temperatures in a   thermostated water bath, representing possible real situations to feed   hydrogen\/oxygen fuel cells. Current results are discussed and correlated   with each particular environmental condition and hydrogen flow rate. It   is found that for some environmental conditions the flow rate discharge   behavior improves significantly","palabras_clave":"HYDROGEN STORAGE|METAL HYDRIDE|HEAT EXCHANGE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64809,"idioma_id":4,"titulo":"Performance improvement on LiFePO4\/C composite cathode for lithium-ion batteries","resumen":"Temperature glycine assisted solid-state synthesis was used to prepare LiFePO4\/C composite samples with two types of material improvements. It will be shown how can addition of a high conductive support as well as doping with supervalent metal ions improve the electrochemical performance of Li-ion cathode. Three samples with different properties were prepared and investigated e pure LiFePO4\/ C with no material improvements, LiFePO4\/C prepared with multi walled carbon nanotubes (MWCNT) conductive support and LiFePO4\/C doped by 1% of cobalt. Glycine was used as inorganic carbon coating precursor during the synthesis of all samples. XRD measurements confirmed production of highly crystalline LiFePO4 cathode material with diameter varying between 40 nm and 200 nm. Electrochemical measurements confirmed increasing the intra-particle conductivity by MWCNT or Co doping. Galvanostatic battery testing shows that LiFePO4\/MWCNT\/C composite delivers highest capacity 130 mA h g1 at C\/5. LiFePO4\/MWCNT\/C cathode material prepared by solid state synthesis exhibit excellent electrochemical performances, improved conductivity, and good rate capability compared to the LiFePO4\/C composite material.","palabras_clave":"Li ion Battery|Cathode|Battery"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64810,"idioma_id":4,"titulo":"Antioxidant defenses and trace metal bioaccumulation capacity of Cymbul a nigra (gastropoda: patellidae","resumen":"The present study deals with the effect of trace  metals on the endangered limpet Cymbula nigra. The  Bay of Algeciras (Strait of Gibraltar) was used as the  study site. Important industrial activity takes place in the  area, including frequent oil spills. However, it is home to  important populations of C. nigra. The objective of this  work was to determine if these animals were being affected  at a subcellular level by the pollutants present in  their environment and to analyze the trace metal  concentrations in the animal\u2019s soft tissues. To determine  the effects of water quality on the antioxidant activity and  concentrations through field experimentation, a total of  six sites were selected in Algeciras Bay, three located in  the inner areas (environmentally degraded sites with  higher levels of pollutants) and three in the outermost  areas of the Bay. Stress associated to reactive oxygen  species formation was assessed on digestive glands and  gills as the enzymatic antioxidant activity of catalase  (CAT), superoxide dismutase (SOD), and glutathione Stransferase  (GST) and as the concentrations of lipidsoluble  (&#945;-tocopherol and &#946;-carotene) and the watersoluble  antioxidants (reduced and oxidized glutathione  (GSH and GSSG)). Gills and digestive glands of those  animals located in the inner areas of Algeciras Bay  showed higher CAT activity values than those located  in the outer areas. As a general pattern, we observed  higher antioxidant activities and concentrations in digestive  glands that in gills, suggesting the possibility that  pollutants are mainly being incorporated by limpets  through the food. As a general rule, larger animals  showed greater concentrations of these compounds.  Iron, zinc, and manganese, in this order, were present in  the tissues at the highest concentrations. Chromium and  manganese were found in significantly higher concentrations  in those animals collected from the inner areas of  the Bay. Through the present study, we provide the first  data regarding the antioxidant defense levels and metal  accumulation capacity of this species, and we reinforce  the idea that this endangered species may be, in fact,  relatively tolerant to degraded environments.","palabras_clave":"Cymbula |Trace metals |Limpet| ROS . |Strait of Gibraltar|Subcellular stress"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64812,"idioma_id":4,"titulo":"Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons","resumen":"The sub-cellular localization of NO generation in soybean cotyledons, and the relationship between NO synthesis and in vivo chloroplast performance were studied. Fluorescence microscopy, employing 4-aminomethyl-2´,7´-difluorofluorescein diacetate (DAF-FM DA), showed a strongly punctuated fluorescence in mesophyll cells. The co-localization of DAF-FM and chlorophyll fluorescence, in confocal laser microscopy images, indicated the presence of NO in the chloroplasts. NO visualization was dependent on light irradiance, seedling age, and chloroplast function throughout cotyledons lifespan cycle. The addition of herbicides with action in chloroplasts (DCMU and paraquat) dramatically affected the quantum yield of photosystem II (fPSII), and lead to images with absence of punctuated green fluorescence. Moreover, electronic paramagnetic resonance signals corresponding to NO-spin trap adduct observed in cotyledon homogenates decreased significantly by the treatment with herbicides, as compared to controls.Neither chloroplast function nor NO content were significantly different in cotyledons from plants growing in the presence of ammonium or nitrate as the nitrogen source. These findings suggest that chloroplasts are organelles that contribute to NO synthesis in vivo, and that their proper functionality is essential for maintaining NO levels in soybean cotyledons. ","palabras_clave":"chloroplasts|nitric oxide|cotyledons|soybean"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64813,"idioma_id":4,"titulo":"High Purity cis-Cinnamic Acid Preparation for Studying Physiological Role of trans-Cinnamic and cis-Cinnamic Acids in Higher Plants","resumen":"Cinnamic acids are present in all kinds of  plant tissues and as consequence in herbs and derived medicines, cosmetics,  foods, etc. The interest in study their role in nature and possible health  effect on consumers has grown exponentially. Because of their molecular  structure they can exist in both trans-  and cis-forms, having both found in  plants. However, as only the trans-forms  are commercially available very few studies in  vitro and in vivo of the cis-role are available. In the present  review the current knowledge in this field of studies is summarized including  the brief description of &nbsp;a new tool for  easy and friendly synthesis of cis-cinnamic  acids by researchers in order to be able to conduct new experiments. ","palabras_clave":"cinnamic acids|cis-cinnamics|photochemistry|ionic liquids"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64814,"idioma_id":4,"titulo":"Photophysical behavior of new acridine(1,8)dione dyes","resumen":"The photophysical behaviorof five acridine(1,8)dione dyes of biological interest was studied by using absorption and fluorescence spectroscopy, photoacousticsand time resolved phosphorescence techniques. Results obtained in ethanol and acetonitrile solutions show that the main spectroscopic andphotophysical parameters of these compounds depend strongly on both the solvent and the oxygen concentration. Oxygencompletely quenched the triplet state of all dyes. In nitrogen-saturatedsolutions quantum efficiencies of triplet formation were higher in ethanol thanin acetonitrile. We propose that Octahydro-ã-piridyl-acridine-1,8-dione inethanol can be used as a calorimetric reference","palabras_clave":"Photochemistry|Dyes|Quinones|Acridines"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64815,"idioma_id":4,"titulo":"Z-Sinapinic Acid. The Change of the Stereochemistry of Cinnamic Acids as Rational Synthesis of a New Matrix for Carbohydrate MALDI-MS analysis,","resumen":"Successful application  of matrix assisted laser desorption\/ionization mass spectrometry (MALDI-MS)  started with the introduction of efficient matrices such as cinnamic acid  derivatives (i.e., 3,5-dimethoxy-4-hydroxycinnamic acid, SA;  á-cyano-4-hydroxycinnamic acid, CHCA). Since the empirical founding of these  matrices other commercial available cinnamic acids with different nature and  location of substituents at benzene ring were attempted. Rational design and  synthesis of new cinnamic acids have been recently described too. Because the  presence of a rigid double bond in its molecule structure cinnamic acids can  exist as two different geometric isomers, the E- and Z-forms.  Commercial available cinnamic acids currently used as matrices are the trans- or E- isomer (E-cinnamic acids or trans-cinnamic acids). As a new rational design of MALDI matrices,  Z-cinnamic acids were synthesized and their properties as matrices  studied.&nbsp; Their performance was compared  with that of the corresponding E-isomer  and classical crystalline matrices (3,5-dihydroxybenzoic acid; norharmane) in  the analysis of neutral\/sulfated carbohydrates. Herein we demonstrate the  outstanding performance for Z-SA.  Sulfated oligosaccharides were detected in negative ion mode and the  dissociation of sulfate groups was almost suppressed. Additionally, to better  understand the quite different performance of each geometric isomer as  matrix&nbsp; the physical and morphological  properties as well as the photochemical stability in solid state were studied.  The influence of the E\/Z photoisomerization  of the matrix during MALDI was evaluated. Finally, molecular modeling (density  functional theory study) of the optimized geometry and stereochemistry of E- and Z-cinnamic acids revealed some factors governing the analyte-matrix interaction. ","palabras_clave":"UV-MALDI-MS|UV-MALDI matrix|cis-SA|trans-SA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64816,"idioma_id":4,"titulo":"Photochemical and Thermal Stability of Some Dihydroxyacetophenones Used as UV-MALDI-MS Matrices","resumen":"&nbsp;    2,4-, 2,5-, 2,6- and 3,5-dihydroxyacetophenone (DHA) used as matrices in  matrix-assisted ultraviolet laser desorption\/ionization mass spectrometry  (UV-MALDI-MS), were studied by steady-state and transient  absorption spectroscopy, together with DFT calculations at the B3LYP level of  theory. All compounds have low fluorescence quantum  yields, possibly due to an efficient excited state intramolecular proton  transfer (ESIPT). Laser flash photolysis (LFP) results  showed that only  for 2,4-dihydroxyacetophenone a phototautomer could be detected at l = 400 nm. Their photochemical  stability in solution at different wavelengths and conditions was analyzed by  UV-vis and 1H nuclear  magnetic resonance spectroscopy (1H-NMR), together with  thin layer chromatography (TLC) and ultraviolet laser  desorption\/ionization mass spectrometry (UV-LDI-MS).  Only 3,5-DHA showed decomposition when irradiated, probably because  phototautomerization is not possible. Thermal stability studies of these compounds in  solid state were also conducted. ","palabras_clave":"UV-MALDI-MS|fenilketone matrix|photochemistry|2-hidroxy-ketophenones"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64817,"idioma_id":4,"titulo":"OXIDATIVE STRESS ASSESSED IN SALIVA FROM PATIENTS WITH","resumen":"  There is evidence that acute myocardial infarction (AMI) is associated  with increasing production of reactive oxygen species and  tissue injury. The aim of this study was to assess the presence of  oxidative stress indices in saliva 24 and 48h after AMI. Materials  and methods: We designed a prospective study comparing salivary  levels of biomarkers of oxidative stress in patients with AMI  with elevation of the ST segment in electrocardiogram versus clinically  healthy subjects. Oxidative stress indices including the rate  of oxidation of 2?7? dichlorohydrofluorescein diacetate (DCFHDA)  and the activity of the antioxidant enzyme catalase (CAT)  were evaluated in saliva from patients with AMI at 24 and 48  hours. At each sampling time, blood was drawn for serum markers  of myocardial infarction. Results: This study included ten  patients with acute ST-segment elevation myocardial infarction  and ten clinically healthy controls. Mean age was 67.8 ± 11.1 vs.  48.7 ± 4.1 years (p&lt;0.001) and gender was 60% male vs. 50%  (p&gt;0.05) for AMI vs. controls, respectively. Our results demonstrated  an increase in the rate of oxidation of DCFH-DA in the  myocardial infarction group as compared with controls (p=0.004),  which remained unchanged at 48h. There was no difference in  salivary catalase activity between controls and AMI subjects at  24h or at 48h post-diagnosis (p=0.157). The relationship between  CAT48 and DCFH-DA48 was fairly significant (r=0.39; p=0.053).  Conclusion: This preliminary study showed that biomarkers of  oxidative stress are detectable in saliva of patients with acute  myocardial infarction. Clinical Relevance: Future studies using a  larger population are needed to confirm these observations and to  explore the possibility of using the saliva to monitor evolving diagnosis  and prognosis in acute coronary syndrome.","palabras_clave":"Estrés oxidativo|saliva|infarto agudo miocardio"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64818,"idioma_id":4,"titulo":"Genetic variants in a lipid regulatory pathway as potential tools for improving the nutritional quality of grass-fed beef","resumen":"The aim of this study was to evaluate the effect of genetic variants on candidate genes  corresponding to the sterol recognition element?binding protein-1 (SREBP-1) signaling  pathway and stearoyl-CoA desaturases (SCD1 and SCD5) on muscle fatty acid (FA)  composition of Brangus steers fattened on grass. FA profiles were measured on Longissimus  lumborum muscle samples using a gas chromatography?flame ionization detection  technique. A total of 43 tag single-nucleotide polymorphisms on the SCD1, SCD5,  SREBP-1, SCAP, INSIG1, INSIG2, MBTPS1, MBTPS2, and SRPR genes were genotyped on  246 steers to perform a marker?trait association study. To evaluate the influence of the  Indicine breed in the composite breed, additional groups of 48 Angus, 18 Hereford, 75  Hereford x Angus, and 36 Limousin x Hereford?Angus steers were also genotyped. To  perform the association analysis, FA data were grouped according to the number of carbon  atoms and\/or number of double bonds (i.e. SFA, MUFA, PUFA, etc.). In addition, different  indexes that reflect the activity of FA desaturase and elongase enzymes were calculated.  SCD1 markers significantly affected C14:1\/(C14:0 + C14:1) and C18:1\/(C18:0 + C18:1)  indexes, whereas one SNP in SCD5 was correlated with the C16:1\/(C16:0 + C16:1) index.  Polymorphisms in the signal recognition particle receptor (SRPR) gene were associated with all  the estimated desaturase indexes. Because the evaluated markers showed no effect on total  lipid content of beef, this work supports the potential utilization of these markers for the  improvement of grass-fed beef without undesirable side effects.","palabras_clave":"beef quality|genetic markers|desaturases|SRBP1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64819,"idioma_id":4,"titulo":"Effect of NDP-alpha-MSH on PPAR-gamma and beta Expression and Anti-Inflammatory Cytokine Release in Rat Astrocytes and Microglia.","resumen":"Brain inflammation plays a central role in numerous brain pathologies. Microglia and astrocytes are the main effector cells that become activated when an inflammatory process takes place within the central nervous system. alpha-melanocytestimulating hormone (alpha-MSH) is a neuropeptide with proven anti-inflammatory properties. It binds with highest affinity to the melanocortin receptor 4 (MC4R), which is present in astrocytes and upon activation triggers anti-inflammatory pathways. The aim of this research was to identify anti-inflammatory mediators that may participate in theimmunomodulatory effects of melanocortins in glial cells. Since peroxisome proliferator-activated receptors (PPARs) have recently been implicated in the modulation of inflammation, we investigated the effect of an alpha-MSH analog, [Nle4, D-Phe7]-alpha-MSH (NDP-alpha-MSH), on PPAR-b and PPAR-g gene and protein expression in rat primary astrocytes and microglia. Weinitially demonstrated that rat primary microglia express MC4R and showed that treatment with NDP-alpha-MSH increases PPAR-g protein levels and strongly decreases PPAR-b levels in both astrocytes and microglia. We also showed that extracellular signal-regulated kinase 1\/2 (ERK1\/2)-mediated signaling is partially involved in these effects in a cell-specific fashion. Finally, we showed that NDP-alpha-MSH stimulates the release of the anti-inflammatory cytokines IL-10 and TGF-b from microglia and astrocytes, respectively. The presented data suggest a role for IL-10 and TGF-b in the protective action of melanocortins and a connection between MC4R pathway and that of the nuclear receptor PPAR-g. This is the first report providing evidence that MC4R is expressed in rat primary microglia and that melanocortins modulate PPAR levels in glialcells. Our findings provide new insights into the mechanisms underlying the activation of glial MC4R and open perspectives for new therapeutic strategies for the treatment of inflammation-mediated brain diseases.","palabras_clave":"MELANOCORTINS|ASTROCYTES|PPARs|MC4 RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64820,"idioma_id":4,"titulo":"Ontogeny and tissue differentiation of the pelvic girdle and hind limbs of anurans","resumen":"We present the ontogeny of the integrated musculoskeletal complex that com-  prises the pelvic girdle and hind limbs of anurans. Our histological data show  that the pelvic girdle originates from a singlemesenchymatic condensation. The  tissue differentiation sequence is cartilage, muscle and tendon. The intrusion of  the ischiadic nerve into the limb bud is produced very early in ontogeny. The  pre-cartilage appears in the pre-motile stage. Therefore, the nerve produces a  movement analogous to the ?embryonic motility? that would induce the emer-  gence of the pre-cartilage. The acetabulum is the first of all cavitation processes  to form, the second one being the knee. The acetabulum appears before the  muscles are mature, although it has been stressed that the muscle contraction  maintains joint progenitors committed to their fate. Our data indicate an explo-  sive differentiation of all 11 muscular masses together. We provide three new  characters that support the monophyly of Hyloides, Acosmanura and Neobatra-  chia.","palabras_clave":"esquelotegenesis|amphibia|sistema apendicular|histogenesis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64821,"idioma_id":4,"titulo":"Activation of H2O2 and superoxide production using a novel cobalt complex based on a polyampholyte","resumen":"A new catalyst based on Co(II) and a hydrogel with property of polyampholyte was characterized by equilibrium studies of Co(II) uptake, solid-state NMR and energy dispersive X-ray analysis. The matrix derived from methacrylic acid and 2-methylimidazole is easily synthesized in one-spot strategy, and combines coordination properties with chemical resistance. The catalytic activity of this material on H2O2 activation was studied by electron spin resonance, which confirmed the release of superoxide radical. A possible mechanism of interaction involves the simultaneous production of dioxygen, protons and water. The catalytic performance was assessed in the activation of H2O2 for the oxidation of two representative organic compounds of environmental concern. About 70% of methyl orange, a model azo dye, was removed from distilled water in 2 h by oxidation with H2O2 60mM and by adsorption on the catalyst. The amount of adsorbed dye was minimized in the presence of 0.1M Na2SO4. The kinetics of the processes followed a pseudo-first-order empirical model, and the catalyst was recovered and reused.Epinephrine was chosen as a pharmaceutical model susceptible to superoxide attack. About 80% of conversion to adrenochrome was reached in less than 6 min, following a pseudo-first-order kinetic model.","palabras_clave":"HETEROGENEOUS CATALYSIS|COBALT COMPLEXES|POLYAMPHOLYTES|SOLID STATE NMR"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64822,"idioma_id":5,"titulo":"Partición diferencial de nutrientes en árboles de Nothofagus antarctica creciendo en un gradiente de calidades de sitio en Patagonia Sur","resumen":"Nothofagus antarctica is a native species that grows in the South of Argentina and Chile. Information of tree nutrient partitioning is essential for evaluating the impacts of silvicultural practices on site productivity, mineral fertility and bioelement recycling. We determined nitrogen, phosphorous, potassium, calcium, sulphur and magnesium partition in plant organs (i.e. root, stem, foliage) by allometric relationships of nutrient partitioning among aerial and below ground N. antarctica components. We used information previously reported of nutrient contents of individual trees across different ages (5-220 years) and crown classes (dominant, codominant, intermediate and suppressed) growing on different site classes. Allometric regressions were fitted for nitrogen, phosphorus, potassium, calcium, sulphur and magnesium aerial-belowground, leaves-roots, stems-roots and leaves-stems. Slopes were compared by ANOVA to detect differences according to site classes. Allometric regression analyses for nutrient partitioning showed good fitness (R2 between 0.77 and 0.99) and allocation varied depending on each particular nutrient and site class. For example, in the best site class, magnesium was the most allocated to aerial components; meanwhile in the worst site the gradient calcium &gt; nitrogen &gt; magnesium &gt; phosphorus = potassium = sulphur was found. Furthermore, all allometric relationships that included roots had lower slopes for worse site classes, indicating higher nutrient allocation to belowground components. Roots were an important sink for most nutrients, and its relative importance increased as site quality decreased. This characteristic to differently allocate resources according to site could be the explanation for the broad distribution of N. antarctica in Patagonia, from wet to drier sites.","palabras_clave":"Patagonian Forest|forest ecology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64823,"idioma_id":4,"titulo":"Biogeographic and geological contexts: New viewpoints on paleoenvironments during the populating of Latin America","resumen":"Se describe, a modo de introducción, el contenido de un volumen especial de la revista, a propósito de la realización de una sesión temática en el V Simposio sobre el poblamiento temprano en América Latina, que tuvo lugar en La Plata en  noviembre 2010.","palabras_clave":"PALEOENVIRONMENTS|LATE PLEISTOCENE|POPULATING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64824,"idioma_id":4,"titulo":"First insights of the electrocatalytical properties of stepped silver electrodes for the hydrogen evolution reaction","resumen":"The electrocatalytic properties of vicinal Ag(11n) surfaces for the hydrogen evolution reaction are investigated. An analysis of the reactivity as a function of the step density is performed. The rate constants for Volmer and Heyrovsky steps are evaluated from potentiostatic transients. A correlation between the anisotropy of hydrogen adsorption energy for different sites with the non-linear dependence on the step density is established","palabras_clave":"VICINAL SURFACES|AG(11N)|HYDROGEN EVOLUTION|STEPS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64825,"idioma_id":4,"titulo":"Current transients for the hydrogen evolution reaction at high overpotentials on silver electrodes in acid solutions: Experiments and Modelling","resumen":"\t\t\t\t\t\t\t\t\t\t\t\tThe hydrogen evolution reaction has been investigated on Ag(1 0 0) by potentiostatic current transientsat high overpotentials (&#1113105; &gt; 0.7 V) in acidic solutions. The reaction proceeds via the Volmer?Heyrovskymechanism, simultaneously coupled with desorption of sulphate\/disulphate anions. Volmer is the ratedetermined step, although the Heyrovsky reaction is only slightly faster. The kinetic parameters havebeen obtained under various conditions of pH and concentration of anions. A catalytic effect due to thepresence of anions has been observed.\t\t\t\t\t\t\t\t\t","palabras_clave":"Hydrogen Evolution Reaction|Catalysis|Volmer?Heyrovsky mechanism|Anions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64826,"idioma_id":4,"titulo":"The Unique Nucleotide Specificity of the  Sucrose Synthase from Thermosynechococcus elongatus","resumen":"        Sucrose synthase catalyzes the  reversible conversion of sucrose and UDP into fructose and UDP-glucose. In  filamentous cyanobacteria, the sucrose cleavage direction plays a key physiological function in carbon metabolism, nitrogen fixation, and stress tolerance. In unicellular strains, the function of sucrose synthase has not been elucidated.  Wereporta detailed biochemical characterization of sucrose synthase from Thermosynechococcus elongatus after the&nbsp; gene was artificially synthesized for optimal expression in Escherichia coli. The  homogeneous recombinant sucrose synthase was highly specific for ADP as substrate, constituting the first one with this unique characteristic,  and strongly suggesting an interaction between sucrose  and glycogen metabolism.  ","palabras_clave":"Carbohydrate metabolism|Glycogen synthesis|Sucrose synthase|Cyanobacteria"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64827,"idioma_id":4,"titulo":"Heterogeneous Catalytic Activity of Platinum Nanoparticles Hosted in Mesoporous Silica Thin Films Modified with Polyelectrolyte Brushes","resumen":"Platinum nanoparticles of 3 nm diameter were included in mesoporous silica thin films by controlling the mesopore surface charge with a short polymer brush. This metal-polymer-mesopore nanocomposite presents high catalytic activity toward ammonia oxidation at low temperatures with 4.5% weight platinum loading. An anomalous partial selectivity toward nitrous oxide is observed for the first time, which can be traced back to the synergy of the particles and modified surface. This effect opens a path toward the design of nanocomposite catalysts with highly controlled environments, in which the size- and function-controlled cavities can be tuned in order to lower the reaction barriers.","palabras_clave":"MESOPORUS FILMS| METAL-POLYMER NANOCOMPOSITES|POLYMER BRUSHES|GOLD CATALYSIS|AMMONIA OXIDATION|NANOCATALYSIS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64828,"idioma_id":4,"titulo":"Electrochemical Determination of the Glass Transition Temperature of Thin Polyelectrolyte Brushes at Solid&#8722;Liquid Interfaces by Impedance Spectroscopy","resumen":"Devising strategies to assess the glass transition temperature (Tg) of polyelectrolyte assemblies at solid?electrolyte interfaces is very important to understand and rationalize the temperature-dependent behavior of polyelectrolyte films in a wide range of settings. Despite the evolving perception of the importance of measuring Tg under aqueous conditions in thin film configurations, its straightforward measurement poses a challenging situation that still remains elusive in polymer and materials science. Here, we describe a new method based on electrochemical impedance spectroscopy (EIS) to estimate the glass transition temperature of planar polyelectrolyte brushes at solid?liquid interfaces. To measure Tg, the charge transfer resistance (Rct) of a redox probe diffusing through the polyelectrolyte brush was measured, and the temperature corresponding to the discontinuous change in Rct was identified as Tg. Furthermore, we demonstrate that impedance measurements not only facilitate the estimation of Tg but also enable a reliable evaluation of the transport properties of the polymeric interface, i.e., determination of diffusion coefficients, close to the thermal transition. We consider that this approach bridges the gap between electrochemistry and the traditional tools used in polymer science and offers new opportunities to characterize the thermal behavior of complex polymeric interfaces and macromolecular assemblies.","palabras_clave":"no posee"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64829,"idioma_id":4,"titulo":"Methicillin-resistant Staphylococcus aureus ST30-SCCmec IVc clone as the major cause of community-acquired invasive infections in Argentina","resumen":"Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)   infections have become a major concern worldwide. We conducted a   prospective multicenter study of invasive CA-MRSA to evaluate clinical   features and genotype of strains causing invasive infections in   Argentina. A total of 55 patients with invasive CA-MRSA infections were   included. Most patients (60%) had bloodstream infections, 42% required   admission to intensive care unit and 16% died. No CA-MRSA isolates were   multiresistant (resistant &#10878;3 classes of antibiotics). All isolates   carried Panton-Valentine leukocidin (PVL) genes and staphylococcal   cassette chromosome (SCCmec) type IV. The majority CA-MRSA strains   belonged to ST30   and had identical pulsed-field gel electrophoresis (PFGE) patterns,   qualifying as a clonal dissemination of a highly transmissible strain.   The main clone recovered from patients with CA-MRSA invasive infections   was genotyped as pulsed-field gel electrophoresis type C-ST30,   SCCmec type IVc-spa type 019, PVL positive. It has become predominant   and replaced the previously described CA-MRSA clone (PFGE type A, ST5,   SCCmec type IV, spa type 311).","palabras_clave":"Methicillin-resistance Community-associated Staphylococcus aureus|MRSA invasive infection|Molecular epidemiology"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64830,"idioma_id":4,"titulo":"Prospective Multicenter Study of Community-Associated Skin and Skin Structure Infections due to Methicillin-Resistant Staphylococcus aureus in Buenos Aires, Argentina","resumen":"Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is now the most common cause of skin and skin structure infections (SSSI) in several world regions. In Argentina prospective, multicenter clinical studies have only been conducted in pediatric populations.","palabras_clave":"Staphylococcus|infections"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64831,"idioma_id":5,"titulo":"Margen bruto de diferentes híbridos de espárrago verde (Asparagus officinalis var. altilis L.), en la provincia de Buenos Aires, Argentina","resumen":"Resumen:   El espárrago representa una alternativa productiva para los agricultores que está creciendo a nivel mundial. Su demanda se centra en el hemisferio norte. Por lo tanto, los países del hemisferio sur que producen este cultivo en la temporada opuesta pueden comercializar su producción en su mercado interno como a nivel internacional. Dado que este vegetal tiene una alta interacción genotipo-ambiente, es necesario evaluar su productividad y el impacto en los resultados económicos. En este sentido, el objetivo de este trabajo fue estudiar la incidencia uso de diferentes híbridos de espárragos verdes en los resultados económicos de la cosecha por medio del cálculo del margen bruto como herramienta en los procesos de adopción de la gestión empresarial en el cuarto año de la productividad. La productividad obtenida con siete híbridos totalmente masculinos de origen italiano: Italo, Zenón, Eros, Ercole, H668, Marte y Giove, en comparación con el control estadounidense UC-157, se evaluó en Azul, Buenos Aires, Argentina. El costo total de producción para el año de evaluación fue $ 5,208 USD. Como el espárrago es un cultivo perenne, una cuota de amortización de $ 473 se consideró USD. Se obtuvo una productividad comercial promedio de 9 t ha-1, en &#8203;&#8203;la que se calculó el margen bruto (EE.UU. $ \/ ha). El GM fue 10.225,7 EE.UU. $ \/ ha con un rango entre 8.903,8 EE.UU. $ \/ ha (Marte híbrido) y 11.746,3 EE.UU. $ \/ ha (Zeno híbrido). Los rendimientos obtenidos con todos los híbridos estudiados permitieron un margen bruto positivo, lo que demuestra que se trata de una alternativa productiva prometedor para la región.","palabras_clave":"PRODUCCION|RENTABILIDAD|TURIONES|MERCADO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64832,"idioma_id":4,"titulo":"New Findings about the Composition and Structure of Ni Nanoparticles Protected with Organomercaptan Molecules","resumen":"Here we explore the synthesis of alkanethiol-coated Ni NPs following the one-phase reaction method by Brust et al.(1) The reduction of NiCl2 with NaBH4 in the presence of dodecanethiol (C12SH) yields a complex product that is difficult to identify as illustrated in the figure of merit. We synthesized Ni(II) dodecanethiolate (C12S) (without the addition of NaBH4) for comparison and performed an exhaustive characterization with TEM, HR-TEM, AFM, MFM, XPS, XRD, UV-vis, magnetism, and FT-IR. It is found that the organic coating is not quite a well-organized self-assembled monolayer (SAM) surrounding the Ni cluster as previously reported.(2, 3) XPS and XRD data show slight differences between both syntheses; however, Ni(II) thiolate appears to be more stable than reduced Ni when exposed to ambient air, indicating the propensity of metallic Ni to oxidize. It has been shown that irradiating with TEM electrons over various metal thiolates leads to nanoparticle formation.(4) We irradiated over Ni(II) thiolate and observed no evidence of NP formation whereas irradiating a reduced Ni sample exhibited an &#8764;3.0 nm nanoparticle diameter. Magnetism studies showed a difference between both samples, indicating ferromagnetic character for the reduced Ni sample. According to our results, the product of the synthesis is comprised of ultrasmall metallic clusters embedded in some form of Ni(II) C 12S. In this work, we open a discussion of the chemical nature of the core and the shell in the synthesis of Ni NPs protected with organomercaptan molecules","palabras_clave":"Nickel|Nanoparticles|Thiols|Synthesis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64833,"idioma_id":4,"titulo":"Understanding the surface chemistry of thiolate protected metallic nanoparticles","resumen":"Metallic nanoparticles (NPs) appear as promising materials to be used in biomedicine, as efficient catalysts and electrocatalysts, and as active elements in electronic and sensing devices. The most common strategy to protect these NPs is by using thiolate self-assembled monolayers (SAMs), a strategy that has proved to be useful to control the physical and chemical properties of extended solid surfaces. However, the knowledge of the structure and chemistry of thiol&#8722;metal interfaces yet remains elusive, although it is crucial for understanding how NPs interact with molecules, biomolecules, and living cells and also for a better design of NP-based devices. This Perspective strives to show the complexity of the thiol&#8722;metal NP interface chemistry and how this changes with the nature of themetallic core.","palabras_clave":"Nanoparticles|Metals|chemistry|structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64834,"idioma_id":4,"titulo":"Surface Chemistry of 4-Mercaptobenzoic Acid Self-Assembled on Ag(111) and Ag Nanoparticles","resumen":"The adsorption of 4-mercaptobenzoic acid (MBA)  on Ag(111) and Ag nanoparticles (AgNPs) has been studied by Xray  photoelectron spectroscopy (XPS), electrochemical techniques,  high-resolution transmission electron microscopy (HRTEM), and  density functional theory (DFT) calculations. Results show that  MBA molecules adsorb intact on the Ag (111) surface via a thiolate  bond, arranged in a (sqrt(3)× 4) lattice with coverage theta= 0.25. The  phase diagram built using DFT data shows that this lattice is more  stable than the dense (sqrt(7) × sqrt(7)) R19.1° MBA lattices on  reconstructed Ag(111) surfaces. No significant amounts of sulfide  were found neither on the Ag(111) surfaces nor on MBA-protected  AgNPs, suggesting that the Ag core@Ag-sulfide shell structure  where thiolates could be grafted cannot be applied to the MBAprotected  AgNPs. Slow degradation of the MBA protective layer in  ambient conditions on the AgNPs results in disulfide formation and AgNP sintering.","palabras_clave":"Silver|Nanoparticles|Chemsitry|Structure"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64835,"idioma_id":4,"titulo":"Surface-diffusion-driven decay of high-aspect-ratio gratings: Existence of morphologically related classes","resumen":"We present numerical and theoretical results concerning the technologically important process of evolution of high-aspect-ratio profiles due to surface diffusion under thermal treatment. We show how a broad class of initial gratings adopt, after a short transient stage, a typical shape that can be accurately described as a curve whose curvature has only two single Fourier modes as a function of the arc-length  parameter. Moreover, we introduce a set of evolution equations for the relevant parameters that accounts very accurately for both morphological and kinetic aspects of the transformation processes for these curves in a wide region in parameter space. Regarding the decay of rectangular gratings, our numerical results show the existence of geometrically related classes that asymptotically approach to the same trajectory in parameter space. Gratings belonging to the same class pass through the same sequence of morphologies before reaching the final equilibrium state.","palabras_clave":"SURFACE|DIFFUSION|DECAY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64836,"idioma_id":4,"titulo":". Hepatic Enzymes Activity in the Fish Prochilodus Lineatus  (Valenciennes, 1836) After Sublethal Cypermethrin Exposure","resumen":"Abstract Prochilodus lineatus, a fish, was exposed to sublethal concentrations of cypermethrin: 0.075, 0.150, and 0.300 lg L-1 and a control group (without cypermethrin) for 96 h. Five specimens were exposed in each concentration for triplicate (n = 60). Hepatic biochemical values and behavioral changes were studied. The results revealed a significantly higher level of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in hepatic tissue at different concentrations of cypermethrin tested compared to control (p0.05). A decrease in total protein was observed in exposed groups but not significantly (p[0.05). This study provides information to know toxic mechanisms of cypermethrin on hepatic enzymes of P. lineatus that are poorly understood.0.300 lg L-1 and a control group (without cypermethrin) for 96 h. Five specimens were exposed in each concentration for triplicate (n = 60). Hepatic biochemical values and behavioral changes were studied. The results revealed a significantly higher level of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in hepatic tissue at different concentrations of cypermethrin tested compared to control (p0.05). A decrease in total protein was observed in exposed groups but not significantly (p[0.05). This study provides information to know toxic mechanisms of cypermethrin on hepatic enzymes of P. lineatus that are poorly understood.Prochilodus lineatus, a fish, was exposed to sublethal concentrations of cypermethrin: 0.075, 0.150, and 0.300 lg L-1 and a control group (without cypermethrin) for 96 h. Five specimens were exposed in each concentration for triplicate (n = 60). Hepatic biochemical values and behavioral changes were studied. The results revealed a significantly higher level of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in hepatic tissue at different concentrations of cypermethrin tested compared to control (p0.05). A decrease in total protein was observed in exposed groups but not significantly (p[0.05). This study provides information to know toxic mechanisms of cypermethrin on hepatic enzymes of P. lineatus that are poorly understood.lg L-1 and a control group (without cypermethrin) for 96 h. Five specimens were exposed in each concentration for triplicate (n = 60). Hepatic biochemical values and behavioral changes were studied. The results revealed a significantly higher level of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in hepatic tissue at different concentrations of cypermethrin tested compared to control (p0.05). A decrease in total protein was observed in exposed groups but not significantly (p[0.05). This study provides information to know toxic mechanisms of cypermethrin on hepatic enzymes of P. lineatus that are poorly understood.","palabras_clave":"PROCHILODUS LINEATUS|HEPATIC ENZYMES|CYPERMETRIN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64837,"idioma_id":5,"titulo":"Estudio experimental de la doble pérdida en un perfil aerodinámico","resumen":"En el presente trabajo se analiza de manera experimental el efecto conocido como ?doble pérdida? sobre un perfil aerodinámico bajo condiciones de flujos turbulentos incidentes. En particular, el perfil utilizado es un Wortmann FX 63-137, utilizado ampliamente en palas de aerogeneradores. El objetivo del trabajo consiste en determinar la configuración fluidodinámica general en la pérdida dinámica del modelo.En primer lugar se caracterizó el perfil mediante un ensayo de cargas (a través de una balanza aerodinámica de dos componentes) en el túnel de viento de capa límite del LaCLyFA. A partir del ensayo se obtuvieron las curvas características del perfil para diferentes números de Reynolds, obteniéndose parámetros significativos como: Clmáx, ángulo de pérdida, etc. Luego, se implementó un mecanismo neumático para generar cambios bruscos de ángulo de ataque del perfil. Una vez realizado, se procedió a los diferentes ensayos de visualizaciones del flujo para los diferentes Reynolds y diferentes velocidades de cambio de ángulo de ataque. Las técnicas de visualización de flujo utilizadas son: catavientos sobre el perfil y en la estela del mismo, pintura sobre el perfil con querosene y oxido de magnesio y, por último, inyección de humo en la corriente libre.Una vez determinada cualitativamente la configuración fluidodinámica, se seleccionaron las zonas donde se midieron velocidades a través de anemometría de hilo caliente con tres sensores de dos componentes en simultáneo (aguas arriba, sobre el perfil y en la estela del mismo).","palabras_clave":"DOBLE PERDIDA|PERDIDA DINAMICA|FX 63137|VISUALIZACION DOBLE PERDIDA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64838,"idioma_id":5,"titulo":"Aplicación de un estimador estadístico de punto de cambio para la detección de eventos turbulentos","resumen":"        El trabajo tiene como objeto presentar la aplicación de un estimador estadístico de punto de cambio para el        análisis y posible detección de eventos turbulentos en el seno de un flujo. Se realiza un estudio a partir de datos        de una señal obtenida de mediciones experimentales. Basados en la metodología de medición de la anemometría        de hilo caliente, se consideran mediciones de las componentes fluctuantes de la velocidad en túnel de viento en        un punto específico. A la muestra de datos obtenidos se le aplican metodologías del estimador estadístico de        punto de cambio, con el fin de detectar los posibles cambios de la fluctuación de la velocidad, estableciendo una        correlación entre las variaciones detectadas y las determinadas mediante las metodologías tradicionales para el        análisis de turbulencia, definiendo escalas y frecuencias características del flujo en el punto de medición. Se        investiga la factibilidad de implementar un código de cálculo que analice la señal permitiendo la detección de los        eventos generados por una estructura vorticosa especifica generada por un dispositivo de control de flujo.","palabras_clave":"TURBULENCIA|FRECUENCIA|ESCALAS|PUNTOS DE CAMBIO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64839,"idioma_id":4,"titulo":"Hybrid hard - and soft - modeling of spectrophotometric data for monitoring  of ciprofloxacin, oxacin and its main photodegradation products at different pH values","resumen":"A simple and fast online spectrophotometric method combined with a   hybrid hard?soft modeling mul-tivariate curve resolution(HS MCR) was   proposed for the monitoring of photodegradation reaction of cipro&#64258;oxacin   under UV radiation. The studied conditions attempt to emulate the   effect of sun light on these antibiotics that could be eventually   present in the environment. The continuous &#64258;ow system made it possible   to study the cipro&#64258;oxacin degradation at different pH values almost at   real time, avoiding errors that could arise from typical batch   monitoring of there action. On the base of a concentration pro&#64257;les   obtained by previous pure soft-modeling approach, reaction pathways have   been proposed for the parent compound and its photo products at   different pH values. These kinetic models were use dasa constraint in   the HS-MCR analysis. The kinetic pro&#64257;les and the corresponding pure   response pro&#64257;le (UV?Vis spectra) of cipro&#64258;oxacin and its main   degradation products were recovered after the application of HS-MCR   analysis to the spectra recorded through out the reaction. The observed   behavior showed a good agreement with the photodegradation studies   reported in the bibliography. Accordingly, the photodegradation reaction   was studied by high performance liquid chromatography coupled with   UV?Vis diode array detector (HPLC-DAD). The spectra recorded during the   chromatographic analysis present a good correlation with the ones   recovered by UV?Vis\/HS-MCR method.","palabras_clave":"CIPROFLOXACIN|PHOTODEGRADATION|MULTIVARIATE CURVE RESOLUTION|HARD-SOFT MODELING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64840,"idioma_id":4,"titulo":"ASF1 Proteins are Involved in UV-induced DNA Damage Repair and are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis thaliana","resumen":"ASF1 is a key histone H3\/H4 chaperone that participates in a variety of DNA and chromatin-related processes, including DNA repair, where chromatin assembly and disassembly is of primaryrelevance. Information concerning the role of ASF1 proteins in post-UV response in higher plants is currently limited. In Arabidopsis thaliana, an initial analysis of in vivo localization of ASF1A andASF1B indicates that both proteins are mainly expressed in proliferative tissues. In silico promoteranalysis identified ASF1A and ASF1B as potential targets of E2F transcription factors. Theseobservations were experimentally validated, both in vitro by electrophoretic mobility shift assays, and in vivo by chromatin immunoprecipitation assays and expression analysis using transgenic plants with altered levels of different E2F transcription factors. These data suggest that ASF1A and ASF1B are regulated during cell cycle progression through E2F transcription factors. In addition, we found that ASF1A and ASF1B are associated with the UV-B induced DNA damage response in A. thaliana. Transcript levels of ASF1A and ASF1B were increased following a UV-B-treatment. Consistent with a potential role in ultraviolet-B (UV-B) response, RNAi silenced plants of both genes showed increased sensitivity to UV-B compared to wild type plants. Finally, by coimmunoprecipitation analysis, we found that ASF1 physically interacts with N-terminal acetylated histones H3 and H4, and with acetyltransferases of the HAM subfamily, which are known to be involved in cell cycle control and DNA repair, among other functions. Together, here we provide evidence that ASF1A and ASF1B are regulated by cell cycle progression and are involved in DNA repair after UV-B irradiation.","palabras_clave":"DNA DAMAGE REPAIR|CHROMATIN REMODELING|CELL CYCLE|PLANTS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64841,"idioma_id":4,"titulo":"DDM1 and ROS1 have a role in UV-B induced- and oxidative DNA damage in A. thaliana","resumen":"Absorption of UV-B by DNA induces the formation of covalent bonds between adjacent pyrimidines. In maize and arabidopsis, plants de&#64257;cient in chromatin remodeling show increased DNA damage compared to WT plants after a UV-B treatment. However, the role of enzymes that participate in DNA methylation in DNA repair after UV-B damage was not previously investigated. In this work, we analyzed how chromatin remodeling activities that have an effect on DNA methylation affects the repair of UV-B damaged DNA using plants de&#64257;cient in the expression of DDM1 and ROS1. First, we analyzed their regulation by UV-B radiation in arabidopsis plants. Then, we demonstrated that ddm1 mutants accumulated more DNA damage after UV-B exposure compared to Col0 plants. Surprisingly,ros1 mutants show less CPDs and 6-4PPs than WT plants after the treatment under light conditions, while the repair under dark conditions is impaired. Transcripts for two photolyases are highly induced by UV-B in ros1 mutants, suggesting that the lower accumulation of photoproducts by UV-B is due to increased photorepair in these mutants. Finally, we demonstrate that oxidative DNA damage does not occur after UV-B exposure in arabidopsis plants; however, ros1 plants accumulate high levels of oxoproducts, while ddm1 mutants have less oxoproducts than Col0 plants, suggesting that both ROS1 and DDM1 have a role in the repair of oxidative DNA damage. Together, our data provide  evidence that both DDM1 andROS1, directly or indirectly, participate in UV-B induced- and oxidative DNA damage repair.","palabras_clave":"UV-B RADIATION|DDM1|ROS1|DNA METHYLATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64842,"idioma_id":4,"titulo":"Identification of a Bifunctional Maize C- and O-Glucosyltransferase","resumen":"Flavonoids accumulate in plant vacuoles usually asO-glycosylated derivatives, but several species can also synthesize flavonoid C-glycosides. Recently, we demonstrated that a flavanone 2-hydroxylase  (ZmF2H1, CYP93G5) converts flavanones to the corresponding 2-hydroxy derivatives, which are expected to serve as substrates for C-glycosylation. Here,weisolated acDNAencoding aUDP-dependent glycosyltransferase (UGT708A6), and its activity was characterized by in vitro and in vivo bioconversion assays. In vitro assays using 2-hydroxyflavanones as substrates and in vivo activity assays in yeast co-expressing ZmF2H1 and UGT708A6 show the formation of the flavones C-glycosides. UGT708A6 can also O-glycosylate flavanones in bioconversion assays in Escherichia coli as well as by in vitro assays with the purified recombinant protein. Thus, UGT708A6 is a bifunctional glycosyltransferase that can produce both C- and O-glycosidated flavonoids, a property not previously described for any other glycosyltransferase.","palabras_clave":"Flavonoids|Glycosyltransferase|Maize"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64843,"idioma_id":4,"titulo":"Nanocellulose patents trends: a comprehensive review on patents on cellulose nanocrystals, microfibrillated and bacterial cellulose","resumen":"Cellulose nanoparticles (i.e. cellulose elements having at least one dimension in the 1-100nm range) have received  increasing attention during the last decade. This is not only evident in academic articles, but it is also manifested  by the increasing number of nanocellulose patents that are published every year. In the current review, nanocellulose patents  are reviewed using specific software which provides valuable information on the annual number of patents that have  been published throughout the years, main patent owners, most prolific inventors, and patents on the field that have received  more citations. Patent statistics on rod-like cellulose nanoparticles extracted from plants by acid hydrolysis (nanocrystals),  mechanical treatment leading to microfibrillated cellulose (MFC), and microbially produced nanofibrils (bacterial  cellulose, BC) are analyzed in detail. The aim of the current review is to provide researchers with patent information  which may help them in visualizing the evolution of nanocellulose technology, both as a whole and also divided among  the different nanosized particles that are currently the subject of outstanding scientific attention. Then, patents are not only  analyzed by their content, but also by global statistics which will reveal the moment at which different cellulose nanoparticles  technologies achieved a breakthrough, the relative interest received by different nanocellulose particles throughout  the years, the companies that have been most interested in this technology, the most prolific inventors, and the patents that  have had more influence in further developments. It is expected that the results showing the explosion that nanocellulose  technology is experiencing in current days will still bring more research on the topic and contribute to the expansion of  nanocellulosics applications.","palabras_clave":"Nanocelllulose|Microcellulose|Bacterial Cellulose"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64844,"idioma_id":4,"titulo":"Effect of different parameters on the hydrolytic activity of cross-linked enzyme aggregates (CLEAs) of lipase from Thermomyces lanuginosa","resumen":"Cross-linked enzyme aggregates (CLEAs) of lipase from Thermomyces lanuginosa (TLL) were synthesized using (NH4)2SO4 as precipitant and glutaraldehyde as cross-linking agent. CLEAs were assayed for their hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.using (NH4)2SO4 as precipitant and glutaraldehyde as cross-linking agent. CLEAs were assayed for their hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.Thermomyces lanuginosa (TLL) were synthesized using (NH4)2SO4 as precipitant and glutaraldehyde as cross-linking agent. CLEAs were assayed for their hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.4)2SO4 as precipitant and glutaraldehyde as cross-linking agent. CLEAs were assayed for their hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant,cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecylsulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respectto TLL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% whencompared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successivelayers of target lipase showed an important increase in their retained activity. The highest recoveredactivity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activityof 75%.","palabras_clave":"CLEAS|LIPASE|LAYERED CLEAS|RECOVERED ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64845,"idioma_id":4,"titulo":"Cross-linked enzyme aggregates (CLEAs) of selected lipases:  a procedure for the proper calculation of their recovered activity","resumen":"In the last few years, synthesis of carrier-free immobilized biocatalysts by cross-linking of enzyme aggregates has appeared as a promising technique. Cross-linked enzyme aggregates (CLEAs) present several  interesting advantages over carrier-bound immobilized enzymes, such as  highly concentrated enzymatic activity, high stability of the produced superstructure, important production costs savings by the absence of a  support, and the fact that no previous purification of the enzyme is  needed. However, the published literature evidences that a) much specific non-systematic exploratory work is being done and, b) recovered activity  calculations in CLEAs still need to be optimized. In this context, this  contribution presents results of an optimized procedure for the  calculation of the activity retained by CLEAs, based on the comparison of their specific activity relative to their free enzyme counterparts. The protocol implies determination of precipitable protein content in  commercial enzyme preparations through precipitation with ammonium  sulphate and a protein co-feeder. The identification of linear ranges of activity versus concentration\/amount of protein in the test reaction is  also required for proper specific activity determinations. By use of mass balances that involve the protein initially added to the synthesis  medium, and the protein remaining in the supernatant and washing  solutions (these last derived from activity measurements), the precipitable protein present in CLEAs is obtained, and their specific  activity can be calculated. In the current contribution the described protocol was applied to CLEAs of Thermomyces lanuginosa lipase, which showed a recovered specific activity of 11.1% relative to native lipase.  The approach described is simple and can easily be extended to other  CLEAs and also to carrier-bound immobilized enzymes for accurate  determination of their retained activity.","palabras_clave":"CLEAs|Lipase|Precipitable protein|Specific actitvity|Recovered activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64846,"idioma_id":4,"titulo":"Dietary fish oil normalized glucose-stimulated insulin secretion in isolated pancreatic islets of dyslipemic  rats through mechanisms involving glucose phosphorylation ,peroxisome proliferator-activated receptor gamma and uncoupling protein 2.","resumen":"This studyevaluatessomepossiblemechanismsbehindthebeneficial effectsofdietary fish oil(FO)on &#946;cell dysfunctioninratsfedasucrose-richdiet(SRD).RatswerefedaSRDfor6months.Thereafter,halfthe ratsreceivedaSRDinwhichcornoilwaspartiallyreplacedbyFOupto8months.Theotherhalfcontinued consumingtheSRDupto8months.Acontrolgroupwasfedacontroldietthroughouttheexperimentalperiod.InisolatedisletsofSRD-fedratsdietaryFOnormalizedthereducedglucosephosphorylation, thealteredglucoseoxidation,thetriglyceridecontent,theincreasedproteinmasslevels ofperoxisomeproliferator-activatedreceptor &#947; (PPAR&#947;) anduncouplingprotein2withoutchangesin GLUT2andPPAR&#945;. These finding suggestthatthechangesmentionedabovecouldbeinvolvedinthenormalization ofthealteredglucose-stimulatedinsulinsecretionpatterninthisnutritionalmodelofdyslipidemia andinsulinresistance.&amp; 2013ElsevierLtd.Allrightsreserved","palabras_clave":"FISH OIL|PANCREATIC ISLETS|PPARS|UCP2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64847,"idioma_id":4,"titulo":"DIETARY CHIA SEED INDUCED CHANGES IN HEPATIC TRANSCRIPTION FACTORS AND THEIR TARGET LIPOGENIC AND OXIDATIVE ENZYME ACTIVITIES IN DYSLIPIDAEMIC INSULIN RESISTANT RATS.","resumen":"The present study analyses the effect of dietary chia seed rich in n-3 alpha-linolenic acid on the mechanisms underlying dyslipidaemia and liver steatosis developed in rats fed a sucrose-rich diet (SRD) for either 3 weeks or 5 months. The key hepatic enzyme activities such as fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G-6-PDH), carnitine palmitoyltransferase-1 (CPT-1) and fatty acid oxidase (FAO) involved in lipid metabolism and the protein mass levels of sterol regulatory element-binding protein-1 (SREBP-1) and PPARalpha were studied. (1) For 3 weeks, Wistar rats were fed either a SRD with 11 % of maize oil (MO) as dietary fat or a SRD in which chia seed replaced MO (SRD+Chia). (2) A second group of rats were fed a SRD for 3 months. Afterwards, half the rats continued with the SRD while for the other half, MO was replaced by chia for 2 months (SRD+Chia). In a control group, maize starch replaced sucrose. Liver TAG and the aforementioned parameters were analysed in all groups. The replacement of MO by chia in the SRD prevented (3 weeks) or improved\/normalised (5 months) increases in dyslipidaemia, liver TAG, FAS, ACC and G-6-PDH activities, and increased FAO and CPT-1 activities. Protein levels of PPARalpha increased, and the increased mature form of SREBP-1 protein levels in the SRD was normalised by chia in both protocols (1 and 2). The present study provides new data regarding some key mechanisms related to the fate of hepatic fatty acid metabolism that seem to be involved in the effect of dietary chia seed in preventing and normalising\/improving dyslipidaemia and liver steatosis in an insulin-resistant rat model. (British J. of Nutr.: Journal of citation Reports Thomson Reuters: IF 3,013-5 años:3,342)","palabras_clave":"DIETARY CHIA SEEDS|DYSLIPIDAEMIA|PPARalpha|STEROL REGULATORY ELEMENT-BINDING PROTEIN-1"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64848,"idioma_id":4,"titulo":"Changes in hepatic lipogenic and oxidative enzymes and glucose homeostasis induced by an acetyl-L-carnitine and nicotinamide treatment in dyslipidaemic insulin resistant rats.","resumen":"1. Normal rats fed a sucrose-rich diet (SRD) develop dyslipidaemia and insulin resistance. The present study examined whether administration of the mitochondrial nutrients nicotinamide and acetyl-l-carnitine reversed or improved these metabolic abnormalities.  2. Male Wistar rats were fed an SRD for 90 days. Half the rats then received daily injections of nicotinamide (25 mg\/kg, i.p.) and acetyl- l-carnitine (50 mg\/kg, i.p.) for a further 90 days. The remaining rats in the SRD-fed group and those in a normal chow-fed control group were injected with an equal volume of saline solution for the same period. The following parameters were determined in all groups: (i) liver activity of fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC) and carnitine-palmitoyl transferase-1 (CPT-1); (ii) hepatic and skeletal muscle triacylglycerol content, plasma glucose, insulin, free fatty acid (FFA) and triacylglycerol levels and pancreatic insulin content; and (iii) glucose tolerance.  3. Administration of nicotinamide and acetyl-l-carnitine to the SRD-fed rats reduced dyslipidaemia, liver steatosis, muscle triacylglycerol content and hepatic FAS and ACC activities and increased CPT-1 activity. In addition nicotinamide and acetyl-l-carnitine improved the glucose disappearance rate (Kg), normalized plasma glucose levels and moderately increased insulinaemia without altering pancreatic insulin content. Finally, nicotinamide and acetyl-l-carnitine administration reduced bodyweight gain and visceral adiposity.  4. The results of the present study suggest that altering key hepatic lipogenic and fatty acid oxidative enzymatic activity could improve dyslipidaemia, liver steatosis and visceral adiposity. Indeed, administration of nicotinamide and acetyl-l-carnitine improved glucose intolerance and normalized plasma glucose levels.(CEPP: Journal of citation Reports Thomson Reuters: IF 2,16-5 años:2,196)","palabras_clave":"ACETYL-L-CARNITINE|NICOTINAMIDE|GLUCOSE HOMEOSTASIS|INSULIN RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64849,"idioma_id":5,"titulo":"REGISTRO FAUNÍSTICO Y PALEOAMBIENTES DEL CUATERNARIO TARDÍO, PROVINCIA DE LA PAMPA, ARGENTINA","resumen":"Resumen. Los mamíferos fósiles del este de la Región Pampeana de Argentina constituyeron la base del esquema cronológico y biostratigráficocontinental del Cenozoico tardío de América del Sur. Se presenta una nueva asociación faunística para el sector occidental de la región,proveniente de Santa Rosa (provincia de La Pampa), se evalúa su contexto sedimentario y se propone un esquema paleoambiental parael área. Esta asociación de mamíferos pleistocenos es la más numerosa y diversa registrada en una misma localidad en la provincia. Está integradapor los siguientes taxones: Glyptodon clavipes Owen, Doedicurus sp. (Xenarthra, Cingulata), Megatherium americanum Cuvier, Glossotheriumrobustum (Owen), Scelidotherium sp. (Xenarthra, Folivora), Toxodon sp., (Notoungulata, Toxodontidae), Lama sp. (Artiodactyla,Camelidae), Equus (Amerhippus) sp. e Hippidion sp. (Perissodactyla, Equidae). Los niveles portadores corresponden a un ambiente canalizadode tipo fluvial que habría funcionado como una zona de escurrimiento que drenaba el exceso de agua pluvial hacia una depresión topográfica(Laguna Don Tomás). Sobre la base de la posición estratigráfica relativa en la secuencia y la correlación con otras localidades de la región,se infiere que los niveles portadores se habrían acumulado durante el Pleistoceno tardío. En el Sitio 1 se registran Glyptodon clavipes, Megatheriumamericanum y Glossotherium robustum, taxones característicos de los Pisos-Edades Bonaerense y Lujanense, y Equus (Amerhippus), taxónexclusivo del Lujanense, por lo que esta nueva asociación es asignable al Piso-Edad Lujanense (Pleistoceno tardío&#8722;Holoceno temprano). Supresencia en estos depósitos corrobora la asignación temporal propuesta a partir de los sedimentos portadores.","palabras_clave":"PLEISTOCENO|LA PAMPA|MEGAMAMIFEROS|PALEOAMBIENTES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64850,"idioma_id":4,"titulo":"On dyadic nonlocal Schrödinger equations with Besov initial data","resumen":"In this paper we consider the pointwise convergence to the initial data for the Schrödinger-Dirac equation $i\tfrac{partial u}{partial t}=D^{eta}u$ with $u(x,0)=u^0$ in a dyadic Besov space. Here $D^{eta}$ denotes the fractional derivative of order $eta$ associated to the dyadic distance $delta$ on $mathbb{R}^+$. The main tools are a summability formula for the kernel of $D^{eta}$ and pointwise estimates of the corresponding maximal operator in terms of the dyadic Hardy-Littlewood function and the Calderón sharp maximal operator.","palabras_clave":"SCHRODINGER EQUATION|BESOV SPACES|HAAR BASIS|NONLOCAL DERIVATIVES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64851,"idioma_id":4,"titulo":"Boundedness of the Hardy-Littlewood maximal operator along the orbits of contractive similitudes","resumen":"In this note we obtain results regarding the preservation of homogeneity   properties along the whole orbit of a given iterated function system   (IFS). We have essentially two types of results. The first class of them   contains negative results: it is possible for a classical IFS to have a   complete non-homogeneous sequence of spaces along the orbit, starting   from very classical homogeneous spaces such as those defined by   Muckenhoupt weights. The second class contains positive results which   can be summarized here by saying that the sequence of spaces defined by   the orbit of contractive similitudes starting at a normal space in the   sense of Ahlfors, Macías, and Segovia, preserves doubling. As a   consequence of these results we conclude boundedness properties of the   Hardy?Littlewood maximal operator along the orbits.","palabras_clave":"Iterated function systems|Spaces of homogeneous nature|Hutchinson orbits|Maximal functions"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64852,"idioma_id":4,"titulo":"Fish oil reverses the altered glucose transporter , phosphorylation, insulin receptor substrate-1 protein level and lipid contents in the skeletal muscle of sucrose-rich diet fed rats.","resumen":"The role and underlying mechanisms by which n3 polyunsaturated fatty acids (PUFA) prevent\/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, weexamined: triglyceride, diacylglycerol, PKC TITA, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids.&nbsp;Rats were fed a SRD during 6 months.Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in whichCO (8% wt\/wt) was replaced by FO (7%þ1% CO) for 2 months. Results were compared with thoseobtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized\/improved lipid storage andPKC TITA protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversedimpaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FOnormalized insulin resistance and increased n3 PUFAs in muscle phospholipids.&amp; 2012 Published by Elsevier Ltd.","palabras_clave":"INSULIN ACTION|DYSLIPIDEMIA|POLYUNSATURATED FATTY ACIDS|SKELETAL MUSCLE"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64853,"idioma_id":4,"titulo":"Lactobacillus rhamnosus CRL1505 beneficially modulates the immuno-coagulative response after pneumococcal infection in immunocompromised malnourished mice","resumen":"Abstract: This work evaluated the effect of orally or nasally administered Lactobacillus rhamnosus CRL1505 on the resistance of immunocompromised protein-malnourished mice to pneumococcal infection. In particular, we aimed to gain knowledge of the mechanism involved in the immunomodulatory effect of L. rhamnosus CRL1505 in malnourished hosts by evaluating its impact on the immuno-coagulative response. Malnutrition significantly increased lung tissue damage caused by Streptococcus pneumoniae infection. Lung damage was associated with a deregulated activation of coagulation and an altered inflammatory response. Pneumococcal colonization of lung and bacteremia were significantly reduced (p < 0.05) in malnourished mice receiving the CRL1505 strain. Moreover, mice repleted with supplemental L. rhamnosus CRL1505 showed the least alteration of the alveolar? capillary barrier and cell damage in lungs after the infectious challenge, especially when the CRL1505 strain was administered by nasal route. Besides, mice treated with L. rhamnosus CRL1505 showed an improved respiratory innate immune response and a lower activation of coagulation. The results of this work indicate that L rhamnosus CRL1505 is able to beneficially modulate the inflammation?coagulation interaction after respiratory infections in malnourished hosts.","palabras_clave":"Lactobacillus rhamnosus CRL1505|malnutrition|respiratory infection|coagulation"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64854,"idioma_id":4,"titulo":"Production of bioemulsifiers by Amycolatopsis tucumanensis DSM 45259 and their potential application in remediation technologies for soils contaminated with hexavalent chromium","resumen":"In recent years, increasing interest has been shown in the use of bioemulsifiers as washing agents thatcan enhance desorption of soil-bound metals. However, high production costs derived from the useof expensive substrates for formulation of the fermentation media represent the main challenge forfull, large-scale implementation of bioemulsifiers. This work reports on a first study of bioemulsifierproduction by the actinobacterium Amycolatopsis tucumanensis DSM 45259 using different carbon andnitrogen sources. Preliminary results on the potential use of these compounds as washing agents for soilscontaminated with Cu(II) and Cr(VI) are also presented. The best specific production was detected usingglycerol and urea as carbon and nitrogen substrates, respectively. However, with all of the substratesused during the batch assay, the bioemulsifiers showed high levels of stability at extreme conditions ofpH, temperature, and salt concentration. Under the current assay conditions, the bioemulsifiers werenot effective in removing Cu(II) from soil. However, they were able to mediate Cr(VI) recovery, with theremoval percentage doubled compared to that seen when using deionized water. These findings appearpromising for the development of remediation technologies for hexavalent chromium compounds basedupon direct use of these microbial emulsifiers.","palabras_clave":"BIOEMULSIFIERS|ACTINOBACTERIUM|HEAVY METALS|SOIL WASHING"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64855,"idioma_id":4,"titulo":"Onset of deep drainage and salt mobilization following forest clearing and cultivation in the Chaco plains (Argentina)","resumen":"Semiarid sedimentary plains occupied by dry forest ecosystems often display low groundwater recharge rates and accumulation of salts in the soil profile. The transformation of these natural systems to rain-fed agriculture has led to raising water tables and a slow, but steady, process of groundwater and soil salinization in vast areas of Australia. In the semiarid plains of Chaco (central South America), unprecedented deforestation rates are taken place. Based on deep soil sampling (0?6 m) in seven paired stands under natural dry forest, rain-fed agriculture and pasture, with different age of clearance (>30 years, 20 and 3 years) in Salta, Argentina, we provide evidence of groundwater recharge increase and onset of salt mobilization in areas where forests were replaced by annual croplands. Soils with higher water and lower chloride content are evidence of deep percolation and salt leaching. In Salta, stands subject to 30 years of rain-fed cultivation had profiles with 30?46% higher moisture content and 94% lower chloride stocks compared to dry forest (0.05&#8201;±&#8201;0.04 kg\/m2 versus 0.77&#8201;±&#8201;0.4 kg\/m2). Estimates of groundwater recharge based on the displacement of chloride peaks suggested values of 27?87 mm yr&#8722;1 for agricultural soybean stands, and 10.4 mm yr&#8722;1 for pastures. While hydrological shifts in the regional groundwater system are poorly monitored and understood, our findings show that it is potentially sensitive to land use changes and to salinization processes.","palabras_clave":"recarga|salinizacion|suelos|deforestacion"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64856,"idioma_id":4,"titulo":"Toll-like receptor 4 D299G polymorphism in metabolic disorders: a meta-analysis","resumen":"The toll-like receptor 4 (TLR4) plays a key role in the activation of innate immune response participating in the recognition of lipopolysaccharides. Changes in the innate immune response are involved in the pathogenesis of some metabolic disorders such as metabolic syndrome and type 2 diabetes mellitus (Met-S and T2DM). It has been recently shown the role of gut microbiota in the perpetuation of both insulin resistance and low-grade chronic inflammation. Some studies have reported that TLR4 D299G polymorphism is associated with metabolic disorders, however results have been inconsistent. Two recent meta-analyses showed that D299G is associated with inflammatory bowel disease and gastrointestinal cancers risk, two pathological states in which the luminal microbial flora-host cells interaction may be implicated. We conducted a systemic review of the published data considering all eligible published studies (six studies with 1696 cases and 3388 controls for D299G) and a meta-analysis was performed to evaluate the association between TLR4 D299G polymorphism and the risk for metabolic disorders. Five studies were identified for T2DM: three corresponding to Caucasian populations and two to mixed populations. The remaining study analyzed Met-S in a Caucasian population. We observed a significant association between D299G polymorphism and metabolic disorders (T2DM and Met-S) risk (OR = 0.566, 95 % CI: 0.347\u20130.925, p = 0.023) particularly in Caucasians. No association was found in mixed population subgroup. Our meta-analysis identified that the AG\/GG genotypes of D299G are associated with decreased metabolic disorders risk.","palabras_clave":"TLR4|D299G|Diabetes|Meta-analysis"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64857,"idioma_id":4,"titulo":"Manganese Induces Mitochondrial Dynamics Impairment and Apoptotic Cell Death. A Study in Human Gli36 Cells","resumen":"The Manganese (Mn) is an essential trace element due to its participation in many physiological processes.However, overexposure to this metal leads to a neurological disorder known as Manganism whose clin-ical manifestations and molecular mechanisms resemble Parkinson?s disease. Several lines of evidenceimplicate astrocytes as an early target of Mn neurotoxicity being the mitochondria the most affectedorganelles. The aim of this study was to investigate the possible mitochondrial dynamics alterations inMn-exposed human astrocytes. Therefore, we employed Gli36 cells which express the astrocytic mark-ers GFAP and S100B. We demonstrated that Mn triggers the mitochondrial apoptotic pathway revealedby increased Bax\/Bcl-2 ratio, by the loss of mitochondrial membrane potential and by caspase-9 acti-vation. This apoptotic program may be in turn responsible of caspase-3\/7 activation, PARP-1 cleavage,chromatin condensation and fragmentation. In addition, we determined that Mn induces deregulationin mitochondria-shaping proteins (Opa-1, Mfn-2 and Drp-1) expression levels in parallel with the dis-ruption of the mitochondrial network toward to an exacerbated fragmentation. Since mitochondrialdynamics is altered in several neurodegenerative diseases, these proteins could become future targets tobe considered in Manganism treatment.","palabras_clave":"APOPTOSIS|ASTROCYTES|MANGANESE|MITOCHONDRIAL DYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64858,"idioma_id":4,"titulo":"Practical and regulatory considerations for stability-indicating methods for the assay of bulk drugs and drug formulations","resumen":"As part of the overall analytical control strategy, current regulations require stability-indicating methods (SIMs) to demonstrate product integrity until the re-test period of drug substances or throughout the shelf life of the drug products. Accordingly, relevant topics related to SIMs (mainly for drug substances but also for drug products) are critically reviewed and some recommendations are given.The development of a SIM is a process that embraces three stages; these entail obtaining suitable samples, selecting the separation technique and choosing the right detection, which also comprises method development and optimization and, finally, validating the method. The first stage yields proper knowledge of the required physicochemical properties of the DS and a deep understanding of its intrinsic stability; these are acquired through stress and accelerated testing, an approach that provides the most appropriate samples for developing SIMs. For small organic molecules, HPLC is the first choice for undertaking the second stage, which entails developing powerful separations with stability-indicating properties. In case of biologics and certain combined products, achieving analytical methods with stability-indicating properties demands a series of methods based on different, orthogonal approaches. Adequate separation of the relevant degradation products from the main analytes requires optimization of a number of chromatographic factors, including column packing, mobile phase composition, the elution mode and other variables. Evaluation of all the stability-related issues demands proper detection systems. Sophisticated hyphenated chromatographic methods are extremely useful for developing powerful SIMs; however, similar chromatographic methodologies with simpler detections are enough for routine use of the SIMs as analytical tools. Full method validation according to official guides, and demonstration of suitability of the SIM for monitoring those products actually formed, finally proves its true stability-indicating power. ","palabras_clave":"STABILITY INDICATING METHODS|DRUG QUALITY AND SAFETY|PHARMACEUTICAL REGULATION|HPLC AND VALIDATION"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64859,"idioma_id":4,"titulo":"Rensch's rule is not verified in melanopline grasshoppers (Acrididae)","resumen":"Sexual size dimorphism (SSD) is almost universal in animals. Rensch?s rule proposes that SSD increases with increasing average body size in taxa where males are larger than females (male- biased SSD; MBSSD) and decreases when females are larger (female biased SSD; FBSSD). Although it was proposed that both patterns are part of the same evolutionary trend, there is more evidence for Rensch?s rule in the first case. We analyzed SSD in the acridid subfamily Melanoplinae in a sample of 718 species and subspecies covering all tribes and representative genera. As in all Orthopera, SSD is female-biased. Body length was used as a proxy for body size. Mean body size within the subfamily varied between 9 and 34.5 mm in males (N= 812) and 12.75 and 44.0 mm in females (N= 735). Except for five species (0.7%) all taxa (from subfamily to subspecies) showed moderate to strong FBSSD (mean= 1.27). The lowest SSD was observed in Melanoplus chumash (SSD= 1.01), and the highest in Phaedrotettix aptera coquinae (SSD= 1.83). To test Rensch?s rule we performed reduced major axis (RMA) regressions between log10 (male body length) and log10 (female body length). In no case RMA slopes were significantly higher than 1.0 which would signal Rensch?s rule. Thus, Melanoplinae represents a new case of FBSSD where Rensch?s rule is not verified. The proximate causes of FBSSD and the non-occurrence of Rensch?s rule in the Orthoptera are discussed as well as the relationship between SSD patterns at the intra- and supraspecific levels.","palabras_clave":"BODY SIZE|MODEL II REGRESSION|SEXUAL SIZE DIMORPHISM|SPORTHROATED GRASSHOPPERS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64860,"idioma_id":5,"titulo":"Murciélagos de la provincia de Córdoba, Argentina: riqueza y distribución","resumen":"Los objetivos del trabajo fueron identificar vacios en la información distribucional existente referida a los quirópteros de la provincia de Córdoba, para determinar el patrón de riqueza de especies e individualizar las variables que afectan la distribución de cada especie en particular. Se realizaron búsquedas bibliográficas de registros publicados, revisiones de ejemplares depositados en colecciones sistemáticas y relevamientos de localidades puntuales. Con base en los datos distribucionales obtenidos se realizaron modelos predictivos utilizando el programa Maxent. El mapa de riqueza se obtuvo mediante la superposición de 10 mapas individuales de presencia-ausencia, más las celdas de presencia de las cinco especies que no pudieron ser modeladas por presentar menos de cinco registros. Se obtuvieron 204 registros de 15 especies en 191 localidades. La mayor parte de las localidades se concentran en la zona serrana. Las 15 especies registradas fueron citadas para el bosque serrano. Desmodus rotundus, Tadarida brasiliensis y Myotis dinellii presentaron el mayor número de registros. En tres de los 26 departamentos de la provincia de Córdoba (General Roca, Roque Sáenz Peña y Totoral) no se hallaron registros. El rango anual de la temperatura fue la variable más importante para el conjunto de especies, seguida por dos variables topográficas, la pendiente y la elevación. El área serrana de la provincia constituyó la zona de mayor riqueza, con celdas con hasta diez especies.","palabras_clave":"Chiroptera|Distribución|Córdoba|Modelos predictivos de nicho"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64861,"idioma_id":5,"titulo":"Descripción morfológica del esqueleto axial de filotinos  (Rodentia: Sigmodontinae: Phyllotini): aspectos morfofuncionales y filogenéticos","resumen":"Los estudios de taxonomía y filogenia en roedores filotinos en particular, y en sigmodontinos en general, se han enfocado en el análisis de las características externas, craneales y dentarias, otorgándole menor importancia al esqueleto postcraneal. Es por esto que se planteó un estudio descriptivo y comparativo de los elementos óseos del esqueleto axial de los representantes de la tribu Phyllotini sobre la base de 140 ejemplares pertenecientes a 10 géneros y 26 especies. Los objetivos del estudio fueron contribuir al conocimiento de la anatomía postcraneal de los filotinos, determinar los morfotipos de las estructuras vertebrales e identificar patrones morfológicos con un probable significado funcional y filogenético. Nuestros resultados indican que el esqueleto axial está formado por 7 vértebras cervicales, 12 ó 13 torácicas, 6 ó 7 lumbares, 4 sacras y 25 a 35 caudales. Cada región presenta distintos patrones morfológicos entre las especies, que podrían vincularse con aspectos tales como la postura, el tipo de locomoción, el uso del hábitat, el tamaño corporal o la historia evolutiva del grupo. El análisis realizado demuestra que el postcráneo en roedores filotinos constituye una valiosa fuente de evidencia para estudios taxonómicos, morfofuncionales y filogenéticos; como en otros grupos de mamíferos, presenta patrones morfológicos que revelan ciertos aspectos de las capacidades locomotoras y permite indicar relaciones entre géneros y especies en concordancia con hipótesis previas sobre la base de otros datos.","palabras_clave":"Anatomía|Phyllotini|Morfología funcional|Filogenia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64862,"idioma_id":4,"titulo":"Electrochemical comparison of two methods for polythionine electropolymerization and its relevance as amperometric detector","resumen":"The electrochemical behavior of polythionine on different electrodes has been investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Polymeric films were obtained by two electropolymerization methods: M1 using cyclic voltammetry or M2 in two steps, a constant potential oxidation and cycle potential sweeps. Those methodologies were compared by analyzing the electrochemical behavior of the films using two redox probes. The modified surfaces were also analyzed as electroanalytical sensors so phenol response was tested and a simple and reliable method for phenol quantification was proposed and validated, corresponding to an M2 glassy carbon modified electrode,using a 0.7 V working potential with a 4 × 10&#8722;6 to 2.7 × 10&#8722;5M linear range, with those conditions a 3.6 × 10&#8722;6 mol L&#8722;1 LOD, 1.09 × 10&#8722;5 mol L&#8722;1 LOQ and a (2.9 ± 0.2) × 107 CPhenol (mol L&#8722;1) sensitivity are obtained.","palabras_clave":"Polythionine|Electropolymerization methods|Phenol"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64863,"idioma_id":4,"titulo":"Determination of Azithromycin by an Alternative FIA Amperometric Method for Different Pharmaceutical Applications","resumen":"This work presents a fast and reliable electroanalytical procedure for the quantification of azithromycin (AZ) in different systems that couples flow injection analysis and amperometric detection (FIA-AM). The quantification was performed at a working potential of 1.20 V (vs Ag\/AgCl\/NaCl(3M)) in 0.05 M phosphate buffer at a flow rate of 1.0 mL min-1. Under these conditions a 1.00 x 10-6 \u2013 1.50 x 10-5 M linear range, (5.8 ± 0.4) x 104 CAZ (µA M -1) sensitivity and 4.44 x 10-7 M limit of detection are obtained. Also, this technique can be used without sample pretreatment and presents good reproducibility. The procedure was applied to the determination of the content of AZ in commercial tablets, to study the intrinsic dissolution rate of compacts of AZ dihydrate, and to determine the AZ release from a AZ hydrogel and carbomer. The method was statistically analyzed and proves to be reliable and robust but more remarkable is that FIA-AM technique presents better sensitivity and high sampling rate.","palabras_clave":"AZITROMICINA|ANALISI POR INYECCION EN FLUJO|AMPEROMETRIA|MUESTRAS FARMACEUTICAS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64864,"idioma_id":4,"titulo":"TRYPANOCIDAL ACTIVITY OF SMALLANTHUS SONCHIFOLIUS. IDENTIFICATION OF ACTIVE SESQUITERPENE LACTONES BY BIOASSAY-GUIDED FRACTIONATION","resumen":"ver adjunto","palabras_clave":"Smallanthus sonchifolius|Lactonas sesquiterpenes|Leishmania"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64865,"idioma_id":4,"titulo":"Natural terpenoids from Ambrosia species are active in vitro and in vivo against human pathogenic trypanosomatids","resumen":"Among the natural compounds, terpenoids play an important role in the drug discovery process for tropical diseases. The  aim of the present work was to isolate antiprotozoal compounds from Ambrosia elatior and A. scabra. The sesquiterpene  lactone (STL) cumanin was isolated from A. elatior whereas two other STLs, psilostachyin and cordilin, and one sterol  glycoside, daucosterol, were isolated from A. scabra. Cumanin and cordilin were active against Trypanosoma cruzi  epimastigotes showing 50% inhibition concentrations (IC50) values of 12 mM and 26 mM, respectively. Moreover, these  compounds are active against bloodstrean trypomastigotes, regardless of the T. cruzi strain tested. Psilostachyin and  cumanin were also active against amastigote forms with IC50 values of 21 mM and 8 mM, respectively. By contrast,  daucosterol showed moderate activity on epimastigotes and trypomastigotes and was inactive against amastigote forms.  We also found that cumanin and psilostachyin exhibited an additive effect in their trypanocidal activity when these two  drugs were tested together. Cumanin has leishmanicidal activity with growth inhibition values greater than 80% at a  concentration of 5 mg\/ml (19 mM), against both L. braziliensis and L. amazonensis promastigotes. In an in vivo model of T.  cruzi infection, cumanin was more active than benznidazole, producing an 8-fold reduction in parasitemia levels during the  acute phase of the infection compared with the control group, and more importantly, a reduction in mortality with 66% of  the animals surviving, in comparison with 100% mortality in the control group. Cumanin also showed nontoxic effects at the  doses assayed in vivo, as determined using markers of hepatic damage.","palabras_clave":"Natural Terpenoids |Ambrosia |Trypanosoma cruzi|Leishmania spp"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64866,"idioma_id":5,"titulo":"Modelo mecanicista empírico para pavimentos flexibles","resumen":"Se presenta un modelo mecanicista empírico, desarrollado en el Laboratorio Vial de la Universidad Nacional de Rosario (Argentina). En este artículo, se comentan las aptitudes del Modelo y se muestra una primera calibración de los sub modelos que lo integran.Dentro de sus características, puede citarse:? Dispone de un Sistema Experto para la modelización por retroajuste.? Simula la evolución de la estructura a lo largo de la vida en servicio, considerando variaciones de tránsito, clima y comportamiento de los materiales.? Considera la simulación de los fenómenos de fisuración por fatiga bajo carga, acumulación de deformaciones plásticas por los distintos materiales y fallas por fisuración térmica.? Permite expresar el comportamiento de la estructura analizada en función de parámetros de superficie, rugosidad, porcentaje de fisuras, porcentaje de baches y ahuellamiento.? Incorpora la opción de análisis con las metodologías mecanicistas clásicas.","palabras_clave":"modelo|diseño mecanicista|comportamiento"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64867,"idioma_id":4,"titulo":"Changes in synaptic transmission and protein expression  in the brains of adult offspring after prenatal inhibition  of the kynurenine pathway","resumen":"During early brain development, N-methyl-D-aspartate (NMDA) receptors are involved in cell migration, neuritogenesis, axon guidance and synapse formation, but the mechanisms which regulate NMDA receptor density and function remain unclear. The kynurenine pathway of tryptophan metabolism includes an agonist (quinolinic acid) and an antagonist (kynurenic acid) at NMDA receptors and we have previously shown that inhibition of the pathway using the kynurenine-3-monoxygenase inhibitor Ro61-8048 in late gestation produces rapid changes in protein expression in the embryos and effects on synaptic transmission lasting until postnatal day 21 (P21). The present study sought to determine whether any of these effects are maintained into adulthood. After prenatal injections of Ro61-8048 the litter was allowed to develop to P60 when some offspring were euthanized and the brains removed for examination. Analysis of protein expression by Western blotting revealed significantly reduced expression of the GluN2A subunit (32%) and the morphogenetic protein sonic hedgehog (31%), with a 29% increase in the expression of doublecortin, a protein associated with neurogenesis. No changes were seen in mRNA abundance using quantitative real-time polymerase chain reaction. Neuronal excitability was normal in the CA1 region of hippocampal slices but paired-pulse stimulation revealed less inhibition at short interpulse intervals. The amount of long-term potentiation was decreased by 49% in treated pups and recovery after low-frequency stimulation was delayed. The results not only strengthen the view that basal, constitutive kynurenine metabolism is involved in normal brain development, but also show that changes induced prenatally can affect the brains of adult offspring and those changes are quite different from those seen previously at weaning (P21). Those changes may be mediated by altered expression of NMDAR subunits and sonic hedgehog.","palabras_clave":"KYNURENINES|NEURODEVELOPMENT|DOUBLECORTIN|HEDGEHOG|D-ASPARTATE|TRYPTOPHAN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64868,"idioma_id":4,"titulo":"High performance collection of cerebrospinal fluid in rats: evaluation of erythropoietin penetration after osmotic opening of the blood-brain barrier","resumen":"An important issue to be considered when studying a new drug for treatment of central nervous system (CNS) diseases is its ability to cross the blood?brain barrier (BBB) and distribute throughout the brain. As cerebrospinal fluid (CSF) has demonstrated to be an invaluable reservoir to study CNS availability of therapeutic proteins, we have developed an improved method for CSF sampling from the cisterna magna of rats. The technique enables the simple and rapid collection of adequate quantities (50?75 &#956;l) of blood-free CSF, rendering a high percentage of animal survival (99%) without clinic or neurological consequences. Its success in avoiding blood contamination of CSF lays in the use of a mixture of lidocaine\/ephinephrine topically injected in the rat´s suboccipital area and neck. Another relevant feature of the methodology is its low cost, since the puncture device can be easily assembled with cheap and available materials and, more importantly, neither expensive stereotaxic equipment nor frame is required. The present method is demonstrated by studying the CSF pharmacokinetics of recombinant human erythropoietin (rhEPO), a well-studied therapeutic candidate for neurological diseases. Moreover, we applied this technique to evaluate a strategy of osmotic disruption of the BBB to achieve a faster delivery of rhEPO into the CNS.","palabras_clave":"CEREBROSPINAL FLUID|BLOOD-BRAIN BARRIER|SAMPLING METHOD|OSMOTIC DISRUPTION|rhEPO"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64869,"idioma_id":4,"titulo":"A versatile ionic strength sensitive tag from a human GM CSF derived linear epitope","resumen":"A 7-mer hGM-CSF-derived linear epitope (APARSPS) is herein described as a novel and small tag taking into account its particular binding affinity in native conditions that could be easily modified by increasing or lowering the ionic strength. Thus, a 3.4 or 3.8-fold binding increment was observed in high NaCl concentration when the tag was fused to IFN-&#945;2b or when the peptide was in its native environment, respectively. The high salt concentration increased the affinity of the binding interaction and improved the APARSPS epitope binding to the paratope allowing one to design an immunoaffinity chromatography purification step in which the high ionic strength was useful to adsorb the fusion protein to the immunoaffinity matrix and the low ionic strength at pH 9 was valuable to desorb it (a 470-fold purification with a 94%-purity was attained in only one step). Also, this short tag did not affect the functionality of the fusion protein and it was able to be detected both in the natural molecule (hGM-CSF) as in the tagged one with the same high detection limit: 273 pg of each protein.","palabras_clave":"EPITOPE TAG|SHORT TAG|hGM-CSF|AFFINITY CHROMATOGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64870,"idioma_id":5,"titulo":"LA ECONOMIA RANQUELINA FRENTE AL REPLIEGUE TERRITORIAL Y POLITICO (1870-1880)","resumen":"A&nbsp; lo&nbsp; largo&nbsp; del&nbsp; siglo&nbsp; XIX&nbsp; los&nbsp; indígenas&nbsp; que&nbsp; habitaban&nbsp; las&nbsp; tierras&nbsp; pampeanas&nbsp; y cordilleranas&nbsp; emplearon,&nbsp; en&nbsp; forma&nbsp; complementaria,&nbsp; estrategias&nbsp; diplomáticas&nbsp; y&nbsp; bélicas en pos de sostener un modelo económico basado en la convivencia de bienes de origen tradicional&nbsp; con&nbsp; otros&nbsp; de&nbsp; procedencia&nbsp; cristiana.&nbsp; Aún&nbsp; cuando&nbsp; gran&nbsp; parte&nbsp; de&nbsp; los&nbsp; últimos fueron reapropiados según&nbsp; las pautas culturales de&nbsp; las tolderías,&nbsp; la economía&nbsp; indígena fue quedando estructurada por la economía nacional. El trabajo se centra en la dinámica de&nbsp; la&nbsp; Frontera&nbsp; Sur&nbsp; cordobesa&nbsp; y&nbsp; trata&nbsp; de&nbsp; explicar&nbsp; de&nbsp; qué&nbsp; manera&nbsp; los&nbsp; procesos&nbsp; de arrinconamiento territorial y político ocurridos en la década de 1870 (avance de la línea militar&nbsp; y&nbsp; repliegue&nbsp; de&nbsp; los&nbsp; malones)&nbsp; impactaron&nbsp; sobre&nbsp; los&nbsp; ranqueles.&nbsp; Su&nbsp; consecuencia más visible fue la modificación de las formas tradicionales de distribución de bienes y la dependencia respecto de las raciones asignadas por los tratados de paz.","palabras_clave":"FRONTERA SUR|ESTADO NACIONAL|RANQUELES|ECONOMIA INDIGENA"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64871,"idioma_id":4,"titulo":"Phylogeography and population genetic structure of the Talas's tuco-tuco (Ctenomys talarum): integrating demographic and habitat histories","resumen":"                                We examined the  phylogeography of the South American subterranean rodent Ctenomys  talarum (Talas?s  tuco-tucos) using mitochondrial DNA (mtDNA) control region (D-loop) sequences.  This species is an herbivorous rodent endemic to the Province of Buenos Aires,  Argentina, that lives in natural grasslands in coastal sand dune habitats and  in some fragmented inland populations. In this study, we 1) assessed the  genetic relationship among populations of C. talarum across the entire  distributional range of this species and 2) analyzed how the geological history  of the habitat has affected the genetic structure and demographic history of  these populations. A complex network of haplotypes in conjunction with the  AMOVA results showed high genetic subdivision and a strong phylogeographic  pattern among populations of C. talarum. Pairwise FST values showed significant  differentiation among all populations studied. The overall pattern was similar  to that expected under the isolation-by-distance model, suggesting equilibrium  between gene flow and local genetic drift. Major geographic barriers (e.g.  rivers and unsuitable habitat) in the area, in conjunction with population isolation,  appeared to be associated with strong genetic differentiation among the  different geographical groups. Local mismatch distributions and tests of  neutrality suggest contrasting histories for different groups of populations;  while some populations appeared to be characterized by demographic stability  and no significant departures from neutrality, others showed departures from  strict neutrality consistent with a recent demographic expansion. Finally, a  close association seems to exist between the major climatic changes that  occurred during the Late Pleistocene and Holocene in the central region of  Argentina and the main historical demographic changes inferred from C.  talarum. Current  populations of C. talarum appear to be relicts of a more extended  historical distribution along the Argentinean pampas. These historical  extinctions, however, have not erased the signature of long-term stability and  geographic structure of this species along the coastal and inland distribution  ranges.","palabras_clave":"CTENOMYS TALARUM|HISTORICAL DEMOGRAPHY|PHYLOGEOGRAPHY|SAND DUNE HABITATS"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64872,"idioma_id":5,"titulo":"Diferenciación geográfica en caracteres de la morfología craneana en el roedor subterráneo Ctenomys australis (Rodentia: Ctenomyidae). Geographic differentiation in skull morphological characters in the subterranean rodent Ctenomys australis (Rodentia: Ct","resumen":"RESUMEN. En este trabajo se evalúa la existencia de diferenciación morfológica asociada a variación geográfica  en el roedor subterráneo Ctenomys australis, el cual se distribuye en la primera franja de dunas costeras  entre las localidades de Necochea y Punta Alta, provincia de Buenos Aires, Argentina. Utilizando Análisis de  Función Discriminante (AFD), se evalúa si la variación geográfica es congruente con un modelo de aislamiento  por distancia y se lo compara con el patrón de diferenciación reportado a partir de marcadores moleculares  como el ADNmt (supuestamente neutral) y las aloenzimas. Se espera que la variación morfológica a nivel geográfico  sea explicada, en gran medida, por los efectos de la deriva, contribuida por tasas de migración bajas  y el propio aislamiento entre localidades. Esta predicción general de aislamiento por distancia no descarta la  presencia de otras fuentes de variación morfológica, como aquella de origen adaptativo (adaptación local) y la  inducida ambientalmente. En principio, se observó un patrón de aislamiento por distancia tanto en las distancias  cuadradas de Mahalanobis y las alozímicas, que estuvieron correlacionadas con las distancias geográficas entre  localidades. Además, se observó que gran parte de la variación fenotípica, como la variación relacionada a la  morfología del cráneo o la variación aloenzimática, podría presentar una asociación con diferentes componentes  de adaptación local y\/o variación ambientalmente inducida, en forma paralela con la señal de expansión  demográfica reciente detectada previamente en el ADNmt. Esto abre la posibilidad a que parte de la variación  morfológica se encuentre sujeta a selección y guarde relación con procesos de adaptación local, manteniendo  tasas evolutivas relativamente rápidas en comparación al genoma mitocondrial, que retiene una fuerte impronta  de la historia demográfica.","palabras_clave":"Rodentia|morfología|cráneo|diferenciación"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64873,"idioma_id":4,"titulo":"Developmental changes in cell wall polysaccharides from sweet cherry (Prunus avium L.) cultivars with contrasting firmness","resumen":"Firmness is a major quality attribute of fresh cherries, and is also a main factor affecting susceptibility to bruising and postharvest rots. In order to identify the factors determining the textural differences between genotypes, we evaluated the solubilization, depolymerization and monosaccharide composition of pectin and hemicelluloses from two cultivars with contrasting firmness (?Sweetheart?, firm and ?Newstar?, soft) at four different developmental stages. Firm ?Sweetheart? cherries had higher contents of cell wall material than soft ?Newstar? fruit. Moderate depolymerization of hemicellulose and tightly bound pectins was detected irrespective of cultivar firmness. The general pattern and extent of uronic acid solubilization was quite similar in both cultivars. Rhamnogalacturonan I (RG-I) seemed to be preferentially solubilized in firm ?Sweetheart? fruit as opposed to tightly bound homogalacturonans (HG) in soft cherries. Pectic polymers with higher neutral sugar to uronic acids ratio were found from early development in soft ?Newstar? fruit. Overall, soft ?Newstar? fruit had reduced wall content and higher branching of tightly bound pectins than firm ?Sweetheart? fruit. These factors may be associated with the varietal differences in cherry firmness.","palabras_clave":"Ontogeny|Pectin|Hemicellulose|Softening"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64874,"idioma_id":4,"titulo":"Temporal Variability of Waves at the Proton Cyclotron Frequency Upstream from Mars:  Implications for Mars Distant Hydrogen Exosphere","resumen":"We report on the temporal variability of the occurrence of waves at thelocal proton cyclotron frequency upstream from the Martian bow shockfrom Mars Global Surveyor observations during the first aerobraking andscience phasing orbit periods. Observations at high southern latitudesduring minimum-to-mean solar activity show that the wave occurrence rateis significantly higher around perihelion\/southern summer solstice thanaround the spring and autumn equinoxes. A similar trend is observed inthe hydrogen (H) exospheric density profiles over the Martian daysideand South Pole obtained from a model including UV thermospheric heatingeffects. In spite of the complexity in the ion pickup and plasma wavegeneration and evolution processes, these results support the idea thatvariations in the occurrence of waves could be used to study thetemporal evolution of the distant Martian H corona and its coupling withthe thermosphere at altitudes currently inaccessible to directmeasurements.","palabras_clave":"SPACE|MAGNETOSPHERES|WAVES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64875,"idioma_id":4,"titulo":"Proton cyclotron waves upstream from Mars: Observations from Mars Global Surveyor","resumen":"We present a study on the properties of electromagnetic plasma waves in the region upstream of the Martian bow shock, detected by the magnetometer and electron reflectometer (MAG \/ ER) onboard the&nbsp;Mars Global Surveyor (MGS) spacecraft during the period known as Science Phasing Orbits (SPO). The frequency of these waves, measured in the MGS reference frame (SC), is close to the local proton cyclotron frequency. Minimum variance analysis (MVA) shows that these ?proton cyclotron frequency&nbsp; waves (PCWs) are characterized ? in the SC frame ? by a left-hand, elliptical polarization and propagate almost parallel to the background magnetic field. They also have a small degree of compressibility and an amplitude that decreases with the increase of the interplanetary magnetic field (IMF) cone angle and radial distance from the planet. The latter result supports the idea that the source of these waves is Mars. In addition, we find that these waves are not associated with the foreshock and their properties (ellipticity, degree of polarization, direction of propagation) do not depend on the IMF cone angle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Empirical evidence and theoretical approaches suggest that most of these observations correspond to the ion?ion right hand (RH) mode originating from the pick-up of ionized exospheric hydrogen. The left-hand (LH) mode might be present in cases where the IMF is almost perpendicular to the Solar Wind direction. PCWs occur in 62% of the time during SPO1 subphase, whereas occurrence drops to 8% during SPO2. Also, SPO1 PCWs preserve their characteristics for longer time periods and have greater degree of polarization and coherence than those in SPO2. We discuss these results in the context of possible changes in the pick-up conditions from SPO1 to SPO2, or steady, spatial inhomogeneities in the wave distribution. The lack of influence from the Solar Wind?s convective electric field upon the location of PCWs indicates that, as suggested by recent theoretical results, there is no clear relation between the spatial distribution of PCWs and that of pick-up ions.","palabras_clave":"SPACE|MAGNETOSPHERES|WAVES"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64876,"idioma_id":5,"titulo":"Imaginarios, biomedicina  y  normatividad:  una  respuesta a los procesos  de  estigmatización  y  discriminación  por  VIH","resumen":"El tratamiento del VIH\/sida, como problemática que ocupa a la salud colectiva, requiere nuevas perspectivas teóricas para su análisis. Objetivo: reconstruir algunas matrices imaginarias, configu- radoras de la infección de VIH, vinculadas con prácticas normativas y con la formación del saber clínico, visibilizar consecuencias de estos procesos y articular estas matrices con las problemáticas de discriminación y estigmatización que pesan sobre el diagnóstico. Materiales y métodos: se efectuaron entrevistas en profundidad a jóvenes entre 18 y 35 años y a profesionales de la salud dedicados al tratamiento del VIH\/sida de hospitales públicos de las ciudades de Santa Fe y Paraná (Argentina). Resultados: el discurso biomédico ?nodal en tanto exigencia de la norma y garante del ejercicio de normalización de los individuos y de las poblaciones a partir del binomio normal\/ patológico? incide en la apropiación de la diagnosis y la convivencia con la infección. Conclusión: a partir de los resultados, es posible comprender los procesos de estigmatización y discrimina- ción como mecanismos privilegiados de estas instancias normativas que median la configuración discursiva del VIH. A su vez, se advierte que el rechazo prevalente en jóvenes a realizarse las pruebas diagnósticas, como las dificultades de acceso a los servicios de salud, es consecuencia de los procesos indicados.","palabras_clave":"VIH\/SIDA|Norma|Imaginario social|Estigmatización|Discriminación|Discurso biomédico"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64877,"idioma_id":4,"titulo":"Magnetohydrodynamics in solar and space physics","resumen":"Because of its proximity, our Sun provides a unique opportunity to perform high resolution observations of its outer layers throughout the whole electromagnetic spectrum. We can also theoretically model most of the fascinating physical phenomena taking place on the Sun, as well as their impact on the solar system.","palabras_clave":"magnetohydrodynamic|turbulence|space physics|Hall effect"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64878,"idioma_id":4,"titulo":"Underage drinking: prevalence and risk factors associated to drinking experiences among Argentinean children","resumen":"The aim of this study was to describe the prevalence and predictors of   alcohol drinking behavior in children. Data were obtained from 367   children, aged 8?12 years (M&nbsp;=&nbsp;10.44 years, SD&nbsp;=&nbsp;1.21 years;   61.9% female) from the city of Córdoba, Argentina. Several scales were   used to assess risk factors, including personality traits, alcohol   expectancy (i.e., beliefs about the consequences of using alcohol), and   perceived peer alcohol use, for alcohol drinking and alcohol drinking   experiences. Hierarchical regression analysis was used to determine the   contribution of multiple risk factors to the quantity of alcohol   consumed. The results showed that 58% of the children had tasted   alcohol, and approximately one-third drank alcohol again after the first   drinking experience. Twelve-year-old children had a significantly   higher prevalence of tasting and drinking alcohol and a significantly   greater frequency and quantity of alcohol consumed than younger   children. Eighty percent of the children who liked alcohol during their   first drinking experience reported that they drank alcohol again. Among   the children who did not like alcohol during their first drinking   experience, only 31% drank alcohol again. Underage drinking usually   occurred under adult supervision in family settings when parents or   other relatives allowed them to drink or were aware of their children´s   drinking. The hierarchical regression analysis showed that being older   and male, having more peers that drink alcohol, having higher levels of   extroversion, and having alcohol expectancy for social facilitation   increased the risk for greater alcohol use. The final model explained   33% of the total variance","palabras_clave":"ALCOHOL USE|PREVALENCE|RISK FACTORS|CHILDREN"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64879,"idioma_id":4,"titulo":"mGlu3 receptor and astrocytes: Partners in neuroprotection","resumen":"Astrocytes are currently studied intensively because of their now highlighted relevance as key players with neurons that modulate a wide range of central functions, from synaptic plasticity and synaptogenesis to regulation of metabolic and neuroinflammatory processes.Since the discovery of mGlu3 receptors on astrocytes, accumulating evidence supports a role of these receptors not only in maintaining synaptic homeostasis and treating psychiatric disorders but also in promoting astrocyte survival in several pathologic conditions. This review focuses on providing up-to-date knowledge regarding effects of activating astroglial mGlu3 receptors on psychiatric disorders, astrocyte and neuronal survival, and neurodegenerative diseases.This article is part of a Special Issue entitled Metabotropic Glutamate Receptors.","palabras_clave":"glutamate metabotropic receptors|astrocytes|neuroprotection"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64880,"idioma_id":4,"titulo":"Identification of a field isolate of Fasciola hepatica resistant to albendazole and susceptible to triclabendazole","resumen":"The experiments described here were designed to characterize the status of susceptibility\/resistance to albendazole (ABZ) and triclabendazole (TCBZ) of a Fasciola hepatica isolate (named CEDIVE isolate) recovered from infected sheep (Gualeguay, Argentina) and maintained under laboratory conditions. Two separate clinical efficacy experiments were performed on sheep artificially infected with the CEDIVE isolate. Experiment 1: Sheep were randomly distributed either in an untreated control group or an ABZ (7.5 mg\/kg) treated group (n= 4 each). Additionally, the systemic exposure of ABZ metabolites was assessed in those ABZ-treated infected animals. In Experiment 2, an untreated control group and a TCBZ (10 mg\/kg) treated group was included (n = 4 each). The fluckicidal efficacy of ABZ and TCBZ was assessed by comparison of the number of flukes recovered from untreated and treated sheep at 15 days post-treatment. The efficacy against the CEDIVE isolate of F. hepatica was 29% (ABZ) and 100% (TCBZ). The plasma drug exposure (expressed as AUC and Cmax) observed in the ABZ treated animals (Experiment 1), was in agreement with data obtained in previous studies, which indicate that the low ABZ efficacy was not related to the quality of the pharmaceutical product and\/or to a low systemic availability of the active drug\/metabolite. The results reported here, clearly show that the CEDIVE isolate of F. hepatica behaves as resistant to ABZ and susceptible to TCBZ.","palabras_clave":"Fasciola hepatica|resistance|albendazole|triclabendazole"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64881,"idioma_id":4,"titulo":"Fasciola hepatica: variations in redial development and cercarial production in relation to the geographic origin of the parasite","resumen":"Abstract ? Two hundred Galba truncatula, originating from a French population, were subjected to singlemiracidium infections with an allopatric isolate (origin, Argentina) of Fasciola hepatica. The control group was constituted by 100 snails coming from the same population and exposed to sympatric miracidia of F. hepatica according to  the same protocol. Snail samples were collected bimonthly from both groups between day 14 and day 112 p.e. (at  20 C) and snail dissections were performed to count free rediae, intraredial morulae and free cercariae. Third and  fourth generation rediae were signi&#64257;cantly more numerous in the allopatric group, while the number of &#64257;rst generation  rediae was signi&#64257;cantly lower. In the sympatric group, the decrease in the number of intraredial morulae in the &#64257;rst,  second and third redial generations was signi&#64257;cantly faster. Free cercariae within the snail body were signi&#64257;cantly more  numerous in the sympatric than in the allopatric groups, whatever the date of snail dissection. The changes in redial  development and cercarial production noted in the Argentinean group might be due to the evolution of South American  &#64258;ukes in a divergent way after the introduction of foreign infected ruminants in this continent from the 15th century.","palabras_clave":"Argentina|Cercaria|Fasciola hepatica|France|Galba truncatula|Miracidium|Redia"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64882,"idioma_id":4,"titulo":"Effects of 'in vivo' administration of baclofen on rat renal tubular function","resumen":"The effects of the in vivo administration of baclofen on renal tubular   transport and aquaporin-2 (AQP2) expression were evaluated. In conscious   animals kept in metabolic cages, baclofen (0.01-1mg\/kg, s.c.) induced a   dose-dependent increment in the urine flow rate (UFR) and in sodium and   potassium excretion, associated with an increased osmolal clearance   (Closm), a diminished urine to plasma osmolality ratio (Uosm\/Posm) and a   decrease in AQP2 expression. The above mentioned baclofen effects on   functional parameters were corroborated by using conventional renal   clearance techniques. Additionally, this model allowed the detection of a   diminution in glucose reabsorption. Some experiments were performed   with water-deprived or desmopressin-treated rats kept in metabolic   cages. Either water deprivation or desmopressin treatment decreased the   UFR and increased the Uosm\/Posm. Baclofen did not change the Uosm\/Posm   or AQP2 expression in desmopressin-treated rats; but it increased the   UFR and diminished the Uosm\/Posm and AQP2 expression in water-deprived   animals. These results indicate that in vivo administration of baclofen   promotes alterations in proximal tubular transport, since glucose   reabsorption was decreased. The distal tubular function was also   affected. The increased Closm indicates an alteration in solute   reabsorption at the ascending limb of the Henle´s loop. The decreased   Uosm\/Posm and AQP2 expression in controls and in water-deprived, but not   in desmopressin-treated rats, lead us to speculate that some effect of   baclofen on endogenous vasopressin availability could be responsible for   the impaired urine concentrating ability, more than any disturbance in   the responsiveness of the renal cells to the hormone.","palabras_clave":"GABAB AGONIS|BACLOFEN|RENAL TUBULAR FUNCTION|AQUAPORIN-2"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64883,"idioma_id":4,"titulo":"Elemental and Isotopic Fingerprint of Argentinean Wheat, Matching Soil, Water and Crop Composition to Differentiate Provenance?.","resumen":"The aim of this study was to investigate if elemental and isotopic signatures of Argentinean wheat can be used to develop a reliable fingerprint to assess its geographical provenance. For this pilot study we used wheat cultivated at three different regions (Buenos Aires, Córdoba and Entre Ríos), together with matching soil and water. Elemental composition was determined by ICP-MS. &#948;13C and &#948;15N were measured by isotopic ratio mass spectrometry (IRMS), while 87Sr\/86Sr ratio was determined using thermal ionization mass spectrometry (TIMS). Wheat samples from three sampling sites were differentiated by the combination of 11 key variables (K\/Rb, Ca\/Sr, Ba, 87Sr\/86Sr, Co, Mo, Zn, Mn, Eu, &#948;13C and Na), demonstrating differences among the three studied regions. The application of Generalized Procrustes Analysis (GPA) showed 99.2 % consensus between cultivation soil, irrigation water and wheat samples, in addition to clear differences between studied areas. Furthermore, canonical correlation analysis (CCA) showed significant correlation between the elemental and isotopic profiles of wheat with those corresponding to both soil and water (r2 = 0.97, p < 0.001; and r2 = 0.96, p < 0.001, respectively). To our knowledge, this is the first report demonstrating the correspondence between soil, water, and wheat samples using different statistical methods, showing that wheat elemental and isotopic compositions are mainly related to soil and irrigation water characteristics of the site of growth.","palabras_clave":"wheat|multielement composition|isotopes|geographical origin"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64884,"idioma_id":4,"titulo":"Accumulation and detoxification dynamic of cyanotoxins in the freshwater shrimp Palaemonetes argentines","resumen":"          The uptake and accumulation of Microcystin-LR  (MC-LR) in the shrimp Palaemonetes  argentinus was investigated using both laboratory and field assays. Shrimps  were exposed in aquarium during 1, 2, 3 and 7 days to 1, 10 and 50 µg L-1  MCLR. Accumulation (0.7 ± 0.2 µg MC-LR g-1)  was observed after three days exposures to 50 µg L-1toxin.  Then, shrimps were relocated in fresh water (free of MCLR) to verify the  detoxification dynamic, showing a drop to 0.18  ± 0.01 µg MCLR g-1 after three days. The  activity of Glutathione-S-Transferase, measured in the microsomal fraction (mGST),  was significantly increased during the exposure period, with further increment during  the detoxification period. Furthermore, cytosolic GST (sGST) and Glutathione  Reductase (GR) increased their activities during detoxification, while inhibition  was observed for Catalase (CAT) with no significant changes for Glutathione  Peroxidase (GPx). Current results suggest that GSH conjugation could be an  important MC detoxification mechanism in P.argentinus  and that MCLR induce oxidative stress in this shrimp.           Field exposures were carried out in San  Roque Reservoir (Córdoba, Argentina) after a cyanobacteria bloom. Nodularin  (Nod) presence was measured for the first time in this waterbody (0.24 ± 0.04 µg L-1),  being the first report of Nod in South America freshwaters. Nod was also  detected in P.argentinus (0.16 ± 0.03  µg g-1) after three weeks of exposure in this reservoir, with the  concomitant activation of mGST, sGST and CAT.           Although internal doses of Nod were low  throughout the exposure, they were enough to cause biochemical disturbances in P. argentinus.           Further laboratory studies on Nod  accumulation and antioxidant responses in P.  argentinus are necessary to fully understand these field results. P. argentinus should be considered a  potential vector for transferring cyanotoxins to higher trophic levels in  aquatic environments.  ","palabras_clave":"Cyanotoxins|LC-MS\/MS|Invertebrates|Bioaccumulation|Enzymatic activity"},{"tipo_produccion_id":1,"anio_publica":2013,"producto_id":64885,"idioma_id":4,"titulo":"The Anrep effect: 100 years later","resumen":"Myocardial stretch elicits a rapid increase in developed force, which is  mainly caused by an increase in myofilament calcium sensitivity (Frank Starling  mechanism). Over the following 10-15 minutes, a second gradual increase in  force takes place. This slow force response to stretch is known to be the  result of an increase in the